MYH1G cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYH1G
Entrez Gene ID	427789
Full Name	myosin, heavy chain 1G, skeletal muscle
Synonyms	N101
General protein information	Preferred Names myosin, heavy chain 1G, skeletal muscle Names myosin heavy chain, skeletal muscle, adult myosin, heavy chain 1G, skeletal muscle myosin, heavy chain 1G, skeletal muscle (similar to human myosin, heavy chain 1, skeletal muscle, adult)
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025141728.1	XP_024997496.1	myosin, heavy chain 1G, skeletal muscle isoform X2
XM_015295221.1	XP_015150707.1	myosin heavy chain, skeletal muscle, adult isoform X1
XM_025141726.1	XP_024997494.1	myosin, heavy chain 1G, skeletal muscle isoform X1
XM_025141727.1	XP_024997495.1	myosin, heavy chain 1G, skeletal muscle isoform X1
NM_001319016.1	NP_001305945.1	myosin, heavy chain 1G, skeletal muscle (similar to human myosin, heavy chain 1, skeletal muscle, adult)
XM_025141729.1	XP_024997497.1	myosin, heavy chain 1G, skeletal muscle isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	MYH1C	NP_001107181.1
Mus musculus (house mouse)	Myh2	NP_001034634.2
Gallus gallus (chicken)	MYH1D	XP_415578.4
Gallus gallus (chicken)	MYH1E	NP_001013415.1
Bos taurus (cattle)	MYH2	NP_001159699.1
	MYH2	XP_002800336.1
Canis lupus familiaris (dog)	MYH2	NP_001070263.1
Gallus gallus (chicken)	MYH1A	NP_001013414.1
Gallus gallus (chicken)	MYH1F	XP_001231409.1
Homo sapiens (human)	MYH2	NP_001093582.1
Rattus norvegicus (Norway rat)	Myh2	NP_001128629.1
Gallus gallus (chicken)	MYH1B	NP_989559.1
Gallus gallus (chicken)	MYH1G	XP_003642358.1

Gene Ontology
Bibliography

Related articles in PubMed

A canonical sequence organization at the 5'-end of the myosin heavy chain genes.
Kropp K, Gulick J, Robbins J
The Journal of biological chemistry261(14)6613-8(1986 May)

The chicken myosin heavy chain family.
Robbins J, Horan T, Gulick J, Kropp K
The Journal of biological chemistry261(14)6606-12(1986 May)

The proteolytic substructure of light meromyosin. Localization of a region responsible for the low ionic strength insolubility of myosin.
Nyitray L, Mocz G, Szilagyi L, Balint M, Lu RC, Wong A, Gergely J
The Journal of biological chemistry258(21)13213-20(1983 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MYH1G gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYH1G cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa86399	NM_001319016.1 Latest version!	Gallus gallus myosin, heavy chain 1G, skeletal muscle (similar to human myosin, heavy chain 1, skeletal muscle, adult) (MYH1G), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa16452	XM_025141729.1 Latest version!	Gallus gallus myosin, heavy chain 1G, skeletal muscle (MYH1G), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa16452	XM_025141728.1 Latest version!	Gallus gallus myosin, heavy chain 1G, skeletal muscle (MYH1G), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa43849	XM_025141727.1 Latest version!	Gallus gallus myosin, heavy chain 1G, skeletal muscle (MYH1G), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa43849	XM_015295221.1 Latest version!	Gallus gallus myosin, heavy chain 1G, skeletal muscle (similar to human myosin, heavy chain 1, skeletal muscle, adult) (MYH1G), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa43849	XM_025141726.1 Latest version!	Gallus gallus myosin, heavy chain 1G, skeletal muscle (MYH1G), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYH1G ( NM_001319016.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001305945.1 Gallus gallus myosin, heavy chain 1G, skeletal muscle (similar to human myosin, heavy chain 1, skeletal muscle, adult) (MYH1G), mRNA.

Price & Availability↑

CloneID	OGa86399
Clone ID Related Accession (Same CDS sequence)	NM_001319016.1
Accession Version	NM_001319016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-11
Organism	Gallus gallus(chicken)
Product	myosin, heavy chain 1G, skeletal muscle (similar to human myosin, heavy chain 1, skeletal muscle, adult)
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY116217.1. On Jan 20, 2016 this sequence version replaced XM_003642310.3. ##Evidence-Data-START## Transcript exon combination :: AY116217.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCTTCTT CAGATGCTGA GATGGCTGTC TTTGGAGAGG CAGCTCCCTA CCTCCGAAAG 
TCAGAGAAGG AAAGAATCGA GGCCCAGAAC AAGCCTTTTG ATGCCAAGTC ATCAGTCTTT 
GTGGCCCATC CAAAGGAATC CTTTGTGAAA GGGACAATCC AGAGCAGGGA AACAGGGAAG 
GTCACTGTCA AGACTGAAGG TGGAGAGACT CTGACTGTGA AGGAAGATCA GGTCTTCTCC 
ATGAACCCTC CCAAGTACGA TAAAATTGAG GACATGGCTA TGATGACCCA CCTCCATGAA 
CCCGCTGTGC CGTACAACCT CAAAGAGCGT TATGCAGCCT GGATGATCTA CACCTACTCG 
GGCCTCTTCT GTGTCACTGT CAACCCCTAC AAGTGGCTGC CGGTGTACAA CCCGGAGGTG 
GGGTTGGCCT ACCGAGGCAA AAAGCGCCAG GAGGCCCCTC CACACATCTT CTCCATCTCT 
GACAACGCCT ATCAGTTCAT GCTGACTGAC CGTGAGAATC AGTCGATCCT GATCACCGGA 
GAATCCGGTG CAGGGAAGAC TGTGAACACG AAACGTGTCA TCCAGTACTT TGCAACAATT 
GCAGCTAGCG GGGAGAAGAA GAAGGAGGAG CAGTCCGGCA AAATGCAGGG AACACTTGAG 
GATCAAATCA TCAGTGCCAA CCCCCTGCTG GAGGCCTTTG GAAATGCCGA GACTGTGAGG 
AATGACAACT CCTCACGCTT TGGTAAATTC ATCAGAATCC ATTTTGGGGC CACAGGCAAA 
CTGGCTTCTG CTGACATTGA AACATATCTG CTGGAAAAGT CCAGGGTTAC TTTCCAGCTC 
AAGGCAGAAA GAAGCTACCA CATCTTTTAT CAGGTCACCT CCAACAAGAA GCCAGAGCTA 
ATAGAAATGC TCCTTATCAC CACCAACCCA TATGACTACC CTTTTGTGAG TCAAGGTGAG 
ATCACTGTCC CCAGCATTGA TGACAAAGAG GAGCTTATGG CTACAGATAG TGCCATTGAC 
ATCCTGGGCT TCACTGCTGA TGAAAAGACT GCCATCTACA AGCTGACAGG GGCTGTCATG 
CACTACGGGA ATTTGAAGTT CAAGCAGAAA CAGCGAGAGG AGCAGGCAGA GCCAGATGGC 
ACAGAAGTGG CTGACAAGGC TGCCTACCTG ATGGGCCTGA ATTCAGCTGA CATGCTCAAG 
GCACTATGCT ACCCTCGAGT CAAAGTTGGG AATGAATATG TGACCAAGGG TCAGACTGTA 
CAACAGGTGC ACAATGCTGT AGGTGCTCTG GCAAAGGCTG TCTATGAGAG GATGTTCTTG 
TGGATGGTTG TTCGCATCAA CCAGCAGCTG GATACCAAGC AGCCCAGGCA GTACTTCATT 
GGTGTCCTGG ACATCGCTGG CTTTGAGATC TTTGATTTCA ACAGCTTCGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT TGTGCTGGAG 
CAAGAGGAGT ACAAAAAGGA AGGCATTGAA TGGACATTCA TTGACTTTGG GATGGACCTG 
GCTGCCTGCA TTGAGCTCAT TGAGAAGCCC ATGGGCATCT TCTCCATCCT GGAGGAGGAG 
TGCATGTTCC CCAAGGCAAC TGACACCTCT TTCAAAAACA AGCTCTATGA CCAGCATCTG 
GGCAAGTCCA GCAACTTCCA GAAGCCCAAG CCTGCCAAAG GCAAGGCTGA GGCCCACTTC 
TCTCTGGTGC ACTATGCTGG CACAGTAGAC TACAACATCA CTGGCTGGCT GGAGAAGAAC 
AAGGACCCCC TGAACGAAAC TGTCATTGGG CTGTACCAGA AATCATCTGT GAAGACACTG 
GCCTTACTGT TTGCCAACTA TGGTGGAGCA GAAGCAGAGG CTAGTGGTGG TGGTGGTGGT 
GGCAAGAAAG GTGGCAAGAA GAAGGGTTCT TCTTTCCAGA CTGTCTCAGC CCTTTTCCGG 
GAGAACCTAA ACAAACTGAT GACCAACCTA CGGAGTACAC ACCCTCATTT TGTCCGCTGC 
ATCATCCCAA ATGAAACAAA AACACCTGGT GCCATGGAGC ATGAACTGGT GCTGCACCAG 
CTGCGCTGTA ACGGCGTGCT GGAGGGGATC AGGATTTGCA GGAAAGGCTT CCCCAGCAGA 
GTCCTCTATG CAGACTTTAA ACAGAGGTAC AAGGTGCTTA ATGCCAGTGC CATCCCTGAG 
GGACAGTTCA TTGATAGCAA GAAAGCTTCT GAGAAGCTTC TTGGGTCAAT TGATGTGGAC 
CACACCCAGT ACAAATTTGG TCACACCAAG GTGTTCTTCA AAGCTGGGCT GCTGGGACTC 
CTGGAGGAGA TGAGAGATGA GAAGCTTGCA CAGATCATCA CTCGCACACA GGCCAGATGC 
AGGGGCTTCC TGATGAGAGT GGAATACCAG AGAATGGTGG AAGGGAGGGA GTCCATCTTC 
TGCATCCAGT ACAATGTTCG TGCATTCATG AATGTCAAGC ACTGGCCCTG GATGAAGCTG 
TTCTTTAAGA TCAAGCCCCT GCTGAAGAGC GCAGAGTCTG AGAAGGAGAT GGCCAACATG 
AAGGAAGAAT TTGAGAAGAC CAAGGAGGAG CTTGCAAAGT CTGAGGCAAA GAGGAAGGAG 
CTGGAGGAGA AAATGGTGAA GCTGGTGCAG GAGAAAAATG ATCTGCAGCT CCAAGTGCAG 
GCTGAAGCTG ATGCTTTGGC TGATGCTGAG GAAAGGTGTG ACCAGCTCAT CAAAACCAAA 
ATCCAGCTGG AAGCCAAAAT TAAGGAGGTG ACAGAACGGG CTGAGGATGA GGAGGAAATT 
AATGCTGAAC TGACAGCCAA GAAGAGGAAA CTGGAGGATG AATGCTCAGA GCTGAAGAAA 
GATATAGATG ACCTAGAGTT AACACTGGCC AAAGTTGAGA AGGAAAAGCA TGCCACTGAA 
AACAAGGTGA AAAACCTCAC AGAAGAGATG GCAGCCCTGG ACGAGACAAT TGTCAAGCTG 
ACAAAAGAGA AGAAAGCCCT CCAAGAGGCC CATCAGCAGA CACTGGATGA CCTGCAGGCT 
GAAGAGGACA AAGTCAATAC GCTGACCAAA GCTAAAACCA AGCTGGAACA GCAAGTGGAC 
GATCTGGAAG GGTCCCTGGA GCAAGAGAAG AAGCTGCGCA TGGACCTCGA GAGAGCTAAG 
AGGAAACTTG AAGGAGACCT GAAGATGTCC CAGGATACCA TAATGGATTT GGAAAATGAT 
AAACAGCAGC TGGATGAGAA ACTGAAGAAG AAAGACTTTG AAATCAGCCA GATCCAGAGC 
AAAATTGAGG ATGAGCAAGC CCTGGGCATG CAGTTACAGA AGAAGATCAA GGAGCTGCAG 
GCCCGTATTG AGGAACTGGA GGAGGAAATC GAGGCAGAGC GAACCTCTCG GGCAAAAGCA 
GAGAAGCACC GAGCTGACCT CTCCAGGGAG CTGGAGGAGA TCAGTGAGCG CCTGGAGGAA 
GCAGGAGGGG CTACAGCGGC TCAGATTGAT ATGAACGAGA AGCGTGAGGC AGAATTTCAG 
AAGATGCGCC GTGACCTCGA AGAGGCCACG CTGCAGCACG AAGCCACGGC TGCTGCCCTG 
CGGAAGAAGC ATGCGGACAG CACAGCAGAG CTTGGGGAGC AGATCGACAA CCTGCAACGA 
GTCAAGCAGA AGCTGGAGAA GGAGAAGAGT GAGCTGAAGA TGGAGATTGA TGACTTGGCC 
AGCAACATGG AGTCTGTCTC CAAAGCTAAG GCAAACCTGG AAAAGATGTG TCGTACTTTA 
GAAGACCAAC TGAGTGAGAT TAAGTCAAAG GAGGAGGAGC ATCAACGCAT GATAAACGAT 
CTCAGCACTC AAAGAGCTCG TCTGCAGACA GAATCTGGTG AATACGCGCG CCAGGCGGAC 
AAAAAAGATG GTTTAATTTC TCAGCTGTCC AGAGGGAAAC AGGCCTTCAC GCAACAGATT 
GAAGAACTAA AGCGGCAACT AGAAGAAGAG ATAAAGGCCA AGAATGCCCT GGCCCACAGC 
TTGCAGTCAG CCCGCCATGA CTGTGATTTG CTCCGGGAAC AATATGAGGA GGAGCTTGAA 
GCCAAGGGTG AGCTGCAGCG TGCCCTGTCC AAGGCCAACA GCGAAGTGGC CCAGTGGAGA 
ACCAAATATG AGACGGATGC TATTCAGCGC ACAGAGGAGC TGGAGGAGGC CAAGAAGAAG 
CTGGCACAGC GCCTGCAGGA TGCCGAGGAA CATGTTGAAG CTTGTGAATG CCAAATGCGC 
TTCCTGGAAA AGACAAAGCA AAGGCTGCAG AATGAGGTGG AGGACCTGAT GATTGACGTG 
GAGCGAGCAA ATGCTGCCTG TGCAGCTCTG GACAAGAAGC AGAAGAACTT TGACAAGATC 
CTGGCAGAGT GGAAGCAGAA GTACGAGGAA ACGCAGGCTG AGCTGGAGGC CTCCCAGAAG 
GAGTCTCGCT CCCTCAGCAC AGAGCTGTTT AAGATGAAGA ATGCCTATGA GGAGTCCCTG 
GACCACCTGG AAACGTTGAA GCGTGAGAAC AAGAACTTGC AGCGGGAGAT TTCTGACCTC 
ACAGAGCAGA TTGCAGAGGG AGGAAAGGCG ATTCATGAGT TGGAGAAAGT CAAGAAGCAG 
ATTGAGCAGG AGAAGTCTGA AATCCAGGCA GCACTGGAGG AAGCTGAGGC CTCCCTGGAG 
CATGAAGAGG GGAAGATCCT GCGCCTCCAA CTGGAGCTCA ACCAGGTCAA GTCTGAGATT 
GACAGGAAGA TAGCAGAGAA AGATGAGGAA ATTGACCAGC TGAAGAGAAA TCACCTCAGA 
ATTGTGGAGT CCTTGCAGAG CAGCCTGGAT GCTGAGATCA GGAGCAGGAA TGAGGCCCTG 
AGGCTGAAGA AGAAGATGGA GGGAGACCTG AATGAAATGG AGATCCAGCT GAGCCACGCT 
AACCGTGTTG CTGCAGAGGC ACAAAAGAAC CTGAGAAACA CACAGGCGGT GTTGAAGGAT 
ACCCAGATAC ATTTGGACGA CGCTCTCAGG ACACAGGAGG ACCTGAAGGA GCAGGTGGCC 
ATGGTGGAGC GCAGAGCAAA CCTGTTGCAG GCTGAGATTG AGGAGCTACG GGCAGCCCTG 
GAGCAGACAG AGCGCTCGAG AAAAGTGGCT GAGCAGGAAC TGATGGATGC AAGTGAGCGT 
GTGCAGCTCC TCCATACCCA GAACACCAGC TTGATCAACA CCAAGAAGAA ACTAGAAACG 
GACATTGCCC AAATTCAGAG TGAAATGGAG GATACCATCC AGGAAGCCCG CAATGCTGAA 
GAGAAGGCCA AGAAGGCCAT CACCGATGCA GCCATGATGG CAGAAGAGCT GAAGAAGGAG 
CAGGACACCA GTGCCCACCT GGAGAGGATG AAGAAGAACC TGGACCAGAC GGTGAAGGAC 
CTGCAGCACC GCCTGGATGA GGCAGAGCAG CTGGCCCTGA AGGGAGGCAA GAAGCAAATT 
CAGAAGCTGG AGGCCAGGGT GCGGGAGCTG GAAGGGGAGG TTGATGCTGA GCAGAAGCGC 
AGCGCTGAAG CCGTGAAGGG TGTGCGCAAG TACGAGAGGA GGGTGAAGGA GCTGACCTAC 
CTGTCTGAGG AAGATCGCAA GAACATTCTT AGACTCCAGG ATCTGGTGGA CAAACTACAG 
ATGAAGGTGA AATCCTACAA GAGACAATCT GAGGAGGCTG AGGAGCTGTC CAATGTCAAC 
CTTTCCAAGT TCCGCAAGAT CCAGCACGAG CTGGAGGAGG CTGAGGAGCG GGCTGACATT 
GCAGAGTCAC AGGTCAACAA GCTCCGAGCA AAGAGCCGGG AATTCCATAG GAGGATTGAA 
GAGGAAGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305945.1
CDS	106..5937
Translation	MASSDAEMAVFGEAAPYLRKSEKERIEAQNKPFDAKSSVFVAHPKESFVKGTIQSRETGKVTVKTEGGETLTVKEDQVFSMNPPKYDKIEDMAMMTHLHEPAVPYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVGLAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAASGEKKKEEQSGKMQGTLEDQIISANPLLEAFGNAETVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLKAERSYHIFYQVTSNKKPELIEMLLITTNPYDYPFVSQGEITVPSIDDKEELMATDSAIDILGFTADEKTAIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLMGLNSADMLKALCYPRVKVGNEYVTKGQTVQQVHNAVGALAKAVYERMFLWMVVRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSSNFQKPKPAKGKAEAHFSLVHYAGTVDYNITGWLEKNKDPLNETVIGLYQKSSVKTLALLFANYGGAEAEASGGGGGGKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRVLYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIDVDHTQYKFGHTKVFFKAGLLGLLEEMRDEKLAQIITRTQARCRGFLMRVEYQRMVEGRESIFCIQYNVRAFMNVKHWPWMKLFFKIKPLLKSAESEKEMANMKEEFEKTKEELAKSEAKRKELEEKMVKLVQEKNDLQLQVQAEADALADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAALDETIVKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKMSQDTIMDLENDKQQLDEKLKKKDFEISQIQSKIEDEQALGMQLQKKIKELQARIEELEEEIEAERTSRAKAEKHRADLSRELEEISERLEEAGGATAAQIDMNEKREAEFQKMRRDLEEATLQHEATAAALRKKHADSTAELGEQIDNLQRVKQKLEKEKSELKMEIDDLASNMESVSKAKANLEKMCRTLEDQLSEIKSKEEEHQRMINDLSTQRARLQTESGEYARQADKKDGLISQLSRGKQAFTQQIEELKRQLEEEIKAKNALAHSLQSARHDCDLLREQYEEELEAKGELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEACECQMRFLEKTKQRLQNEVEDLMIDVERANAACAALDKKQKNFDKILAEWKQKYEETQAELEASQKESRSLSTELFKMKNAYEESLDHLETLKRENKNLQREISDLTEQIAEGGKAIHELEKVKKQIEQEKSEIQAALEEAEASLEHEEGKILRLQLELNQVKSEIDRKIAEKDEEIDQLKRNHLRIVESLQSSLDAEIRSRNEALRLKKKMEGDLNEMEIQLSHANRVAAEAQKNLRNTQAVLKDTQIHLDDALRTQEDLKEQVAMVERRANLLQAEIEELRAALEQTERSRKVAEQELMDASERVQLLHTQNTSLINTKKKLETDIAQIQSEMEDTIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLDQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVDAEQKRSAEAVKGVRKYERRVKELTYLSEEDRKNILRLQDLVDKLQMKVKSYKRQSEEAEELSNVNLSKFRKIQHELEEAEERADIAESQVNKLRAKSREFHRRIEEEE

Target ORF information:

RefSeq Version	NM_001319016.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus myosin, heavy chain 1G, skeletal muscle (similar to human myosin, heavy chain 1, skeletal muscle, adult) (MYH1G), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001319016.1

ORF Insert Sequence:

ATGGCTTCTT CAGATGCTGA GATGGCTGTC TTTGGAGAGG CAGCTCCCTA CCTCCGAAAG 
TCAGAGAAGG AAAGAATCGA GGCCCAGAAC AAGCCTTTTG ATGCCAAGTC ATCAGTCTTT 
GTGGCCCATC CAAAGGAATC CTTTGTGAAA GGGACAATCC AGAGCAGGGA AACAGGGAAG 
GTCACTGTCA AGACTGAAGG TGGAGAGACT CTGACTGTGA AGGAAGATCA GGTCTTCTCC 
ATGAACCCTC CCAAGTACGA TAAAATTGAG GACATGGCTA TGATGACCCA CCTCCATGAA 
CCCGCTGTGC CGTACAACCT CAAAGAGCGT TATGCAGCCT GGATGATCTA CACCTACTCG 
GGCCTCTTCT GTGTCACTGT CAACCCCTAC AAGTGGCTGC CGGTGTACAA CCCGGAGGTG 
GGGTTGGCCT ACCGAGGCAA AAAGCGCCAG GAGGCCCCTC CACACATCTT CTCCATCTCT 
GACAACGCCT ATCAGTTCAT GCTGACTGAC CGTGAGAATC AGTCGATCCT GATCACCGGA 
GAATCCGGTG CAGGGAAGAC TGTGAACACG AAACGTGTCA TCCAGTACTT TGCAACAATT 
GCAGCTAGCG GGGAGAAGAA GAAGGAGGAG CAGTCCGGCA AAATGCAGGG AACACTTGAG 
GATCAAATCA TCAGTGCCAA CCCCCTGCTG GAGGCCTTTG GAAATGCCGA GACTGTGAGG 
AATGACAACT CCTCACGCTT TGGTAAATTC ATCAGAATCC ATTTTGGGGC CACAGGCAAA 
CTGGCTTCTG CTGACATTGA AACATATCTG CTGGAAAAGT CCAGGGTTAC TTTCCAGCTC 
AAGGCAGAAA GAAGCTACCA CATCTTTTAT CAGGTCACCT CCAACAAGAA GCCAGAGCTA 
ATAGAAATGC TCCTTATCAC CACCAACCCA TATGACTACC CTTTTGTGAG TCAAGGTGAG 
ATCACTGTCC CCAGCATTGA TGACAAAGAG GAGCTTATGG CTACAGATAG TGCCATTGAC 
ATCCTGGGCT TCACTGCTGA TGAAAAGACT GCCATCTACA AGCTGACAGG GGCTGTCATG 
CACTACGGGA ATTTGAAGTT CAAGCAGAAA CAGCGAGAGG AGCAGGCAGA GCCAGATGGC 
ACAGAAGTGG CTGACAAGGC TGCCTACCTG ATGGGCCTGA ATTCAGCTGA CATGCTCAAG 
GCACTATGCT ACCCTCGAGT CAAAGTTGGG AATGAATATG TGACCAAGGG TCAGACTGTA 
CAACAGGTGC ACAATGCTGT AGGTGCTCTG GCAAAGGCTG TCTATGAGAG GATGTTCTTG 
TGGATGGTTG TTCGCATCAA CCAGCAGCTG GATACCAAGC AGCCCAGGCA GTACTTCATT 
GGTGTCCTGG ACATCGCTGG CTTTGAGATC TTTGATTTCA ACAGCTTCGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT TGTGCTGGAG 
CAAGAGGAGT ACAAAAAGGA AGGCATTGAA TGGACATTCA TTGACTTTGG GATGGACCTG 
GCTGCCTGCA TTGAGCTCAT TGAGAAGCCC ATGGGCATCT TCTCCATCCT GGAGGAGGAG 
TGCATGTTCC CCAAGGCAAC TGACACCTCT TTCAAAAACA AGCTCTATGA CCAGCATCTG 
GGCAAGTCCA GCAACTTCCA GAAGCCCAAG CCTGCCAAAG GCAAGGCTGA GGCCCACTTC 
TCTCTGGTGC ACTATGCTGG CACAGTAGAC TACAACATCA CTGGCTGGCT GGAGAAGAAC 
AAGGACCCCC TGAACGAAAC TGTCATTGGG CTGTACCAGA AATCATCTGT GAAGACACTG 
GCCTTACTGT TTGCCAACTA TGGTGGAGCA GAAGCAGAGG CTAGTGGTGG TGGTGGTGGT 
GGCAAGAAAG GTGGCAAGAA GAAGGGTTCT TCTTTCCAGA CTGTCTCAGC CCTTTTCCGG 
GAGAACCTAA ACAAACTGAT GACCAACCTA CGGAGTACAC ACCCTCATTT TGTCCGCTGC 
ATCATCCCAA ATGAAACAAA AACACCTGGT GCCATGGAGC ATGAACTGGT GCTGCACCAG 
CTGCGCTGTA ACGGCGTGCT GGAGGGGATC AGGATTTGCA GGAAAGGCTT CCCCAGCAGA 
GTCCTCTATG CAGACTTTAA ACAGAGGTAC AAGGTGCTTA ATGCCAGTGC CATCCCTGAG 
GGACAGTTCA TTGATAGCAA GAAAGCTTCT GAGAAGCTTC TTGGGTCAAT TGATGTGGAC 
CACACCCAGT ACAAATTTGG TCACACCAAG GTGTTCTTCA AAGCTGGGCT GCTGGGACTC 
CTGGAGGAGA TGAGAGATGA GAAGCTTGCA CAGATCATCA CTCGCACACA GGCCAGATGC 
AGGGGCTTCC TGATGAGAGT GGAATACCAG AGAATGGTGG AAGGGAGGGA GTCCATCTTC 
TGCATCCAGT ACAATGTTCG TGCATTCATG AATGTCAAGC ACTGGCCCTG GATGAAGCTG 
TTCTTTAAGA TCAAGCCCCT GCTGAAGAGC GCAGAGTCTG AGAAGGAGAT GGCCAACATG 
AAGGAAGAAT TTGAGAAGAC CAAGGAGGAG CTTGCAAAGT CTGAGGCAAA GAGGAAGGAG 
CTGGAGGAGA AAATGGTGAA GCTGGTGCAG GAGAAAAATG ATCTGCAGCT CCAAGTGCAG 
GCTGAAGCTG ATGCTTTGGC TGATGCTGAG GAAAGGTGTG ACCAGCTCAT CAAAACCAAA 
ATCCAGCTGG AAGCCAAAAT TAAGGAGGTG ACAGAACGGG CTGAGGATGA GGAGGAAATT 
AATGCTGAAC TGACAGCCAA GAAGAGGAAA CTGGAGGATG AATGCTCAGA GCTGAAGAAA 
GATATAGATG ACCTAGAGTT AACACTGGCC AAAGTTGAGA AGGAAAAGCA TGCCACTGAA 
AACAAGGTGA AAAACCTCAC AGAAGAGATG GCAGCCCTGG ACGAGACAAT TGTCAAGCTG 
ACAAAAGAGA AGAAAGCCCT CCAAGAGGCC CATCAGCAGA CACTGGATGA CCTGCAGGCT 
GAAGAGGACA AAGTCAATAC GCTGACCAAA GCTAAAACCA AGCTGGAACA GCAAGTGGAC 
GATCTGGAAG GGTCCCTGGA GCAAGAGAAG AAGCTGCGCA TGGACCTCGA GAGAGCTAAG 
AGGAAACTTG AAGGAGACCT GAAGATGTCC CAGGATACCA TAATGGATTT GGAAAATGAT 
AAACAGCAGC TGGATGAGAA ACTGAAGAAG AAAGACTTTG AAATCAGCCA GATCCAGAGC 
AAAATTGAGG ATGAGCAAGC CCTGGGCATG CAGTTACAGA AGAAGATCAA GGAGCTGCAG 
GCCCGTATTG AGGAACTGGA GGAGGAAATC GAGGCAGAGC GAACCTCTCG GGCAAAAGCA 
GAGAAGCACC GAGCTGACCT CTCCAGGGAG CTGGAGGAGA TCAGTGAGCG CCTGGAGGAA 
GCAGGAGGGG CTACAGCGGC TCAGATTGAT ATGAACGAGA AGCGTGAGGC AGAATTTCAG 
AAGATGCGCC GTGACCTCGA AGAGGCCACG CTGCAGCACG AAGCCACGGC TGCTGCCCTG 
CGGAAGAAGC ATGCGGACAG CACAGCAGAG CTTGGGGAGC AGATCGACAA CCTGCAACGA 
GTCAAGCAGA AGCTGGAGAA GGAGAAGAGT GAGCTGAAGA TGGAGATTGA TGACTTGGCC 
AGCAACATGG AGTCTGTCTC CAAAGCTAAG GCAAACCTGG AAAAGATGTG TCGTACTTTA 
GAAGACCAAC TGAGTGAGAT TAAGTCAAAG GAGGAGGAGC ATCAACGCAT GATAAACGAT 
CTCAGCACTC AAAGAGCTCG TCTGCAGACA GAATCTGGTG AATACGCGCG CCAGGCGGAC 
AAAAAAGATG GTTTAATTTC TCAGCTGTCC AGAGGGAAAC AGGCCTTCAC GCAACAGATT 
GAAGAACTAA AGCGGCAACT AGAAGAAGAG ATAAAGGCCA AGAATGCCCT GGCCCACAGC 
TTGCAGTCAG CCCGCCATGA CTGTGATTTG CTCCGGGAAC AATATGAGGA GGAGCTTGAA 
GCCAAGGGTG AGCTGCAGCG TGCCCTGTCC AAGGCCAACA GCGAAGTGGC CCAGTGGAGA 
ACCAAATATG AGACGGATGC TATTCAGCGC ACAGAGGAGC TGGAGGAGGC CAAGAAGAAG 
CTGGCACAGC GCCTGCAGGA TGCCGAGGAA CATGTTGAAG CTTGTGAATG CCAAATGCGC 
TTCCTGGAAA AGACAAAGCA AAGGCTGCAG AATGAGGTGG AGGACCTGAT GATTGACGTG 
GAGCGAGCAA ATGCTGCCTG TGCAGCTCTG GACAAGAAGC AGAAGAACTT TGACAAGATC 
CTGGCAGAGT GGAAGCAGAA GTACGAGGAA ACGCAGGCTG AGCTGGAGGC CTCCCAGAAG 
GAGTCTCGCT CCCTCAGCAC AGAGCTGTTT AAGATGAAGA ATGCCTATGA GGAGTCCCTG 
GACCACCTGG AAACGTTGAA GCGTGAGAAC AAGAACTTGC AGCGGGAGAT TTCTGACCTC 
ACAGAGCAGA TTGCAGAGGG AGGAAAGGCG ATTCATGAGT TGGAGAAAGT CAAGAAGCAG 
ATTGAGCAGG AGAAGTCTGA AATCCAGGCA GCACTGGAGG AAGCTGAGGC CTCCCTGGAG 
CATGAAGAGG GGAAGATCCT GCGCCTCCAA CTGGAGCTCA ACCAGGTCAA GTCTGAGATT 
GACAGGAAGA TAGCAGAGAA AGATGAGGAA ATTGACCAGC TGAAGAGAAA TCACCTCAGA 
ATTGTGGAGT CCTTGCAGAG CAGCCTGGAT GCTGAGATCA GGAGCAGGAA TGAGGCCCTG 
AGGCTGAAGA AGAAGATGGA GGGAGACCTG AATGAAATGG AGATCCAGCT GAGCCACGCT 
AACCGTGTTG CTGCAGAGGC ACAAAAGAAC CTGAGAAACA CACAGGCGGT GTTGAAGGAT 
ACCCAGATAC ATTTGGACGA CGCTCTCAGG ACACAGGAGG ACCTGAAGGA GCAGGTGGCC 
ATGGTGGAGC GCAGAGCAAA CCTGTTGCAG GCTGAGATTG AGGAGCTACG GGCAGCCCTG 
GAGCAGACAG AGCGCTCGAG AAAAGTGGCT GAGCAGGAAC TGATGGATGC AAGTGAGCGT 
GTGCAGCTCC TCCATACCCA GAACACCAGC TTGATCAACA CCAAGAAGAA ACTAGAAACG 
GACATTGCCC AAATTCAGAG TGAAATGGAG GATACCATCC AGGAAGCCCG CAATGCTGAA 
GAGAAGGCCA AGAAGGCCAT CACCGATGCA GCCATGATGG CAGAAGAGCT GAAGAAGGAG 
CAGGACACCA GTGCCCACCT GGAGAGGATG AAGAAGAACC TGGACCAGAC GGTGAAGGAC 
CTGCAGCACC GCCTGGATGA GGCAGAGCAG CTGGCCCTGA AGGGAGGCAA GAAGCAAATT 
CAGAAGCTGG AGGCCAGGGT GCGGGAGCTG GAAGGGGAGG TTGATGCTGA GCAGAAGCGC 
AGCGCTGAAG CCGTGAAGGG TGTGCGCAAG TACGAGAGGA GGGTGAAGGA GCTGACCTAC 
CTGTCTGAGG AAGATCGCAA GAACATTCTT AGACTCCAGG ATCTGGTGGA CAAACTACAG 
ATGAAGGTGA AATCCTACAA GAGACAATCT GAGGAGGCTG AGGAGCTGTC CAATGTCAAC 
CTTTCCAAGT TCCGCAAGAT CCAGCACGAG CTGGAGGAGG CTGAGGAGCG GGCTGACATT 
GCAGAGTCAC AGGTCAACAA GCTCCGAGCA AAGAGCCGGG AATTCCATAG GAGGATTGAA 
GAGGAAGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH1G ( XM_025141728.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024997496.1 Gallus gallus myosin, heavy chain 1G, skeletal muscle (MYH1G), transcript variant X3, mRNA.

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CloneID	OGa16452
Clone ID Related Accession (Same CDS sequence)	XM_025141728.1 , XM_025141729.1
Accession Version	XM_025141728.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	myosin, heavy chain 1G, skeletal muscle isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006105.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTT CAGATGCTGA GATGGCTGTC TTTGGAGAGG CAGCTCCCTA CCTCCGAAAG 
TCAGAGAAGG AAAGAATCGA GGCCCAGAAC AAGCCTTTTG ATGCCAAGTC ATCAGTCTTT 
GTGGCCCATC CAAAGGAATC CTTTGTGAAA GGGACAATCC AGAGCAGGGA AACAGGGAAG 
GTCACTGTCA AGACTGAAGG TGGAGAGACT CTGACTGTGA AGGAAGATCA GGTCTTCTCC 
ATGAACCCTC CCAAGTACGA TAAAATTGAG GACATGGCCA TGATGACCCA CCTCCATGAA 
CCCGCTGTGC TGTACAACCT CAAAGAGCGT TATGCAGCCT GGATGATCTA CACCTACTCG 
GGCCTCTTCT GTGTCACTGT CAACCCCTAC AAGTGGCTGC CGGTGTACAA CCCGGAGGTG 
GTGTTGGCCT ACCGAGGCAA AAAGCGCCAG GAGGCCCCTC CACACATCTT CTCCATCTCT 
GACAACGCCT ATCAGTTCAT GCTGACTGAC CGTGAGAATC AGTCGATCCT GATCACCGGA 
GAATCCGGTG CAGGGAAGAC TGTGAACACG AAACGTGTCA TCCAGTACTT TGCAACAATT 
GCAGCTAGTG GGGAGAAGAA GAAGGAGGAG CAGTCTGGCA AAATGCAGGG AACACTTGAG 
GATCAAATCA TCAGTGCCAA CCCCCTGCTG GAGGCCTTTG GAAATGCCAA GACTGTGAGG 
AATGACAACT CCTCACGCTT TGGTAAATTC ATCAGAATCC ATTTTGGGGC CACAGGCAAA 
CTGGCTTCTG CTGACATTGA AACATATCTG CTGGAAAAGT CCAGGGTTAC TTTCCAGCTC 
AAGGCAGAAA GAAGCTACCA CATCTTTTAT CAGGTCACCT CCAACAAGAA GCCAGAGCTA 
ATAGAAATGC TCCTTATCAC CACCAACCCA TATGACTACC CTTTTGTGAG TCAAGGTGAG 
ATCACTGTCC CCAGCATTGA TGACAAAGAG GAGCTTATGG CTACAGATAG TGCCATTGAC 
ATCCTGGGCT TCACTGCTGA TGAAAAGACT GCCATCTACA AGCTGACAGG GGCTGTCATG 
CACTACGGGA ATTTGAAGTT CAAGCAGAAA CAGCGAGAGG AGCAGGCAGA GCCAGATGGC 
ACAGAAGTGG CTGACAAGGC TGCCTACCTG ATGGGCCTGA ATTCAGCTGA CATGCTCAAG 
GCACTATGCT ACCCTCGAGT CAAAGTTGGG AATGAATATG TGACCAAGGG TCAGACTGTA 
CAACAGGTGC ACAATGCTGT AGGTGCTCTG GCAAAGGCTG TCTATGAGAG GATGTTCTTG 
TGGATGGTTG TTCGCATCAA CCAGCAGCTG GATACCAAGC AGCCCAGGCA GTACTTCATT 
GGTGTCCTGG ACATCGCTGG CTTTGAGATC TTTGATTTCA ACAGCTTCGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT TGTGCTGGAG 
CAAGAGGAGT ACAAAAAGGA AGGCATTGAA TGGACATTCA TTGACTTTGG GATGGACCTG 
GCTGCCTGCA TTGAGCTCAT TGAGAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCAAC CGACACCTCT TTCAAGAACA AGCTCTATGA CCAGCATCTG 
GGCAAGTCCA GCAACTTCCA GAAGCCCAAG CCTACCAAAG GAAAGGTTGA GGCCCACTTC 
TCCCTAATAC ACTATGCTGG CACAGTGGAC TACAATATCA CTGGCTGGCT TGAGAAGAAC 
AAGGACCCCC TGAATGAAAC TGTCATTGGG CTGTACCAGA AATCTTCACT GAAAACTTTG 
GCCTTACTGT TTGCCAACTA TGGTGGAGCA GAAGCAGAGG CTAGTGCTGG TGCTGGCAAG 
AAAGGTGGCA AGAAGAAAGG TTCTTCTTTC CAGACTGTCT CAGCTCTTTT CCGGGAGAAC 
TTAAACAAGC TGATGACCAA TCTGAGGAGC ACCCACCCCC ATTTTGTACG CTGTATCATC 
CCAAATGAAA CAAAAACACC TGGTGCCATG GAGCATGAGC TGGTTCTGCA CCAACTGAGG 
TGTAATGGTG TGCTGGAAGG GATCAGAATT TGCAGGAAAG GATTTCCCAA CAGAGTCCTG 
TATGCAGATT TTAAACAGAG ATACAAAGTA CTAAATGCAA GCGCTATTCC AGAGGGACAG 
TTCATTGACA GCAAGAAGGC TTGTGAGAAA CTGCTTGGAT CAATTGATAT AGACCACACT 
CAGTATAAAT TTGGTCACAC CAAGGTGTTC TTCAAAGCTG GGCTGGTTGG CCTTTTGGAG 
GAGATGAGGG ATGAGAAGCT GGCACAGCTC ATCACTCGCA CACAGGCCAG GTGCAGAGGC 
TTCCTGATGA GAGTGGAATA CCAGAAAATG GTGGAGAGGA GAGAGTCTGT CTTCTGCATC 
CAGTACAACA TTCGTGCATT CATGAATGTC AAGCACTGGC CCTGGATGAA GCTGTTCTTT 
AAGATCAAGC CGCTGCTGAA GAGCGCAGAG TCTGAGAAGG AGATGGCCAA CATGAAACAA 
GAGTTTGAGA AGACCAAGGA AGAGCTTGCA AAGTCTGAGG CAAAGAGGAA GGAGCTGGAG 
GAGAAAATGG TGAAGCTGGT GCAGGAGAAA AATGATCTGC AGCTCCAAGT GCAGGCTGAA 
GCTGATGCAT TGGCTGATGC TGAGGAAAGA TGTGACCAGC TCATCAAAAC CAAAATCCAG 
CTGGAAGCCA AAGTAAAGGA GGTGACAGAA CGGGCTGAGG ATGAGGAGGA AATTAATGCT 
GAACTGACAG CCAAGAAGAG AAAACTGGAG GATGAATGTT CAGAACTGAA GAAAGACATA 
GATGACCTGG AGTTAACATT GGCCAAAGTT GAGAAGGAAA AGCATGCCAC TGAAAACAAG 
GTGAAAAACC TCACAGAGGA GATGGCAGCC CTGGATGAGA CCATTGTGAA GCTGACAAAA 
GAGAAGAAAG CCCTCCAAGA GGCCCATCAG CAGACACTGG ATGACCTGCA GGCTGAAGAG 
GACAAAGTCA ATACGCTGAC CAAAGCTAAA ACCAAGCTGG AACAGCAAGT GGATGATCTG 
GAAGGGTCCC TGGAGCAAGA GAAGAAGCTG CGCATGGACC TCGAGAGAGC CAAGAGGAAA 
CTTGAAGGAG ACCTGAAGTT GGCCCATGAT AGCATAATGG ACTTGGAAAA TGATAAACAG 
CAGCTGGATG AGAAACTGAA GAAGAAAGAC TTTGAAATCA GCCAGATCCA GAGCAAAATT 
GAGGATGAAC AACTTCTGGG CATGCAGTTA CAGAAGAAGA TCAAGGAGCT GCAGGCCCGT 
ATTGAAGAAC TGGAGGAGGA AATTGAGGCA GAGCGAACCT CTCGGGCAAA AGCAGAGAAG 
CACCGAGCTG ACCTCTCCAG GGAGCTGGAG GAGATCAGTG GGCGCCTGGA GGAAGCAGGA 
GGGGCTACAG CAGCTCAGAT CGAGATGAAC AAGAAGCGTG AGGCAGAATT TCAGAAGATG 
CGCCGTGACC TCGAAGAGGC CACGCTGCAG CACGAAGCCA CGGCTGCTGC CCTGCGGAAG 
AAGCATGCGG ACAGCACAGC GGAGCTTGGG GAGCAGATTG ACAACCTGCA ACGAGTCAAG 
CAGAAGCTGG AGAAGGAGAA GAGTGAGCTG AAGATGGAGA TTGATGACTT GGCCAGCAAC 
ATGGAGTCTG TCTCCAAAGC TAAGGCAAAC CTGGAGAAGA TGTGTCGCAC TTTGGAAGAC 
CAACTAAGTG AATTCAAAAC GAAAGATGAG CAGAACCAGC GCATGATCAG TGATCTCAGT 
GCTCAAAGAG CTCGCCTGCA AACAGAATCT GGTGAATACG CACGCCAGGC GGACGAAAAA 
GATGGTTTAA TTTCTCAGCT GTCCAGAGGG AAACAGGCCT TCACGCAACA GATTGAAGAA 
CTAAAGCGGC AACTAGAAGA AGAGATAAAG GCCAAGAATG CCCTGGCCCA CAGCTTGCAG 
TCAGCCCGCC ATGACTGTGA TTTGCTCCGG GAACAATATG AGGAGGAGCT TGAAGCCAAG 
GGTGAGCTGC AGCGTGCCCT GTCCAAGGCC AACAGCGAAG TGGCCCAGTG GAGAACCAAA 
TATGAGACGG ATGCTATTCA GCGCACAGAG GAGCTGGAGG AGGCCAAGAA GAAGCTGGCA 
CAGCGCCTGC AGGATGCCGA GGAACATGTT GAAGCTGTGA ATGCCAAATG CGCTTCCCTG 
GAAAAGACAA AGCAGAGGCT GCAGAATGAG GTGGAGGACC TGATGATTGA CGTGGAGCGA 
GCAAATGCTG CCTGTGCAGC TCTGGACAAG AAGCAGAAGA ACTTTGACAA GATCCTGGCA 
GAGTGGAAGC AGAAGTACGA GGAAACGCAG GCTGAGCTGG AGGCCTCCCA GAAGGAGTCT 
CGCTCCCTCA GCACAGAGCT GTTTAAGATG AAGAATGCCT ATGAGGAGTC CCTGGACCAC 
CTGGAAACGC TGAAGCGTGA GAACAAGAAC TTGCAGCAGG AGATTTCTGA CCTCACAGAG 
CAGATTGCAG AGGGAGGAAA GGCGATTCAT GAGTTGGAGA AAGTCAAGAA GCAGATTGAG 
CAGGAGAAGT CTGAAATCCA GGCAGCACTG GAGGAAGCTG AGGCCTCCCT GGAACATGAA 
GAGGGGAAGA TCCTGCGCCT CCAACTGGAG CTCAACCAGG TCAAGTCTGA GATCGACAGG 
AAGATAGCAG AGAAAGATGA GGAAATTGAC CAGCTGAAGA GAAATCACCT CAGAATTGTG 
GAGTCCTTGC AGAGCAGCCT GGATGCTGAG ATCAGGAGCA GGAATGAGGC CCTGAGGCTG 
AAGAAGAAGA TGGAGGGAGA CCTGAATGAA ATGGAGATCC AGCTGAGCCA CGCTAACCGT 
GTTGCTGCAG AGGCACAAAA GAACCTGAGA AACACACAGG CAGTGTTGAA GGATACCCAG 
ATACATTTGG ACGACGCTCT CAGGACACAG GAGGACCTGA AGGAGCAGGT GGCCATGGTG 
GAGCGCAGAG CAAACCTGTT GCAGGCTGAG ATTGAGGAGC TACGGGCAGC CCTGGAGCAG 
ACAGAGCGCT CGAGAAAAGT GGCTGAGCAG GAACTGATGG ATGCAAGTGA GCGTGTGCAG 
CTCCTCCATA CCCAGAACAC CAGCTTGATC AACACCAAGA AGAAACTAGA AACGGACATT 
GCCCAAATTC AGAGTGAAAT GGAGGATACC ATCCAGGAAG CCCGCAATGC TGAAGAGAAG 
GCCAAGAAGG CCATCACCGA TGCAGCCATG ATGGCAGAAG AGCTGAAGAA GGAGCAGGAC 
ACCAGTGCCC ACCTGGAGAG GATGAAGAAG AACCTGGACC AGACGGTGAA GGACCTGCAG 
CACCGCCTGG ATGAGGCAGA GCAGCTGGCC CTGAAGGGAG GCAAGAAGCA AATTCAGAAG 
CTGGAGGCCA GGGTGCGGGA GCTGGAAGGG GAGGTTGATG CTGAGCAGAA GCGCAGCGCT 
GAAGCCGTGA AGGGTGTGCG CAAGTACGAG AGGAGGGTGA AGGAGCTGAC CTACCAGTCT 
GAGGAAGATC GCAAGAACAT TCTTAGACTC CAGGATCTGG TGGACAAACT ACAGATGAAG 
GTGAAATCCT ACAAGAGACA ATCTGAGGAG GCTGAGGAGC TGTCCAATGT CAACCTTTCC 
AAGTTCCGCA AGATCCAGCA CGAGCTGGAG GAGGCTGAGG AGCGGGCTGA CATTGCAGAG 
TCACAGGTCA ACAAGCTCCG AGCAAAGAGC CGGGAATTCC ATAGGAGGAT TGAAGAGGAA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024997496.1
CDS	6268..12093
Translation	MASSDAEMAVFGEAAPYLRKSEKERIEAQNKPFDAKSSVFVAHPKESFVKGTIQSRETGKVTVKTEGGETLTVKEDQVFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVLAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAASGEKKKEEQSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLKAERSYHIFYQVTSNKKPELIEMLLITTNPYDYPFVSQGEITVPSIDDKEELMATDSAIDILGFTADEKTAIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLMGLNSADMLKALCYPRVKVGNEYVTKGQTVQQVHNAVGALAKAVYERMFLWMVVRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSSNFQKPKPTKGKVEAHFSLIHYAGTVDYNITGWLEKNKDPLNETVIGLYQKSSLKTLALLFANYGGAEAEASAGAGKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPNRVLYADFKQRYKVLNASAIPEGQFIDSKKACEKLLGSIDIDHTQYKFGHTKVFFKAGLVGLLEEMRDEKLAQLITRTQARCRGFLMRVEYQKMVERRESVFCIQYNIRAFMNVKHWPWMKLFFKIKPLLKSAESEKEMANMKQEFEKTKEELAKSEAKRKELEEKMVKLVQEKNDLQLQVQAEADALADAEERCDQLIKTKIQLEAKVKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAALDETIVKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAHDSIMDLENDKQQLDEKLKKKDFEISQIQSKIEDEQLLGMQLQKKIKELQARIEELEEEIEAERTSRAKAEKHRADLSRELEEISGRLEEAGGATAAQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSTAELGEQIDNLQRVKQKLEKEKSELKMEIDDLASNMESVSKAKANLEKMCRTLEDQLSEFKTKDEQNQRMISDLSAQRARLQTESGEYARQADEKDGLISQLSRGKQAFTQQIEELKRQLEEEIKAKNALAHSLQSARHDCDLLREQYEEELEAKGELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERANAACAALDKKQKNFDKILAEWKQKYEETQAELEASQKESRSLSTELFKMKNAYEESLDHLETLKRENKNLQQEISDLTEQIAEGGKAIHELEKVKKQIEQEKSEIQAALEEAEASLEHEEGKILRLQLELNQVKSEIDRKIAEKDEEIDQLKRNHLRIVESLQSSLDAEIRSRNEALRLKKKMEGDLNEMEIQLSHANRVAAEAQKNLRNTQAVLKDTQIHLDDALRTQEDLKEQVAMVERRANLLQAEIEELRAALEQTERSRKVAEQELMDASERVQLLHTQNTSLINTKKKLETDIAQIQSEMEDTIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLDQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVDAEQKRSAEAVKGVRKYERRVKELTYQSEEDRKNILRLQDLVDKLQMKVKSYKRQSEEAEELSNVNLSKFRKIQHELEEAEERADIAESQVNKLRAKSREFHRRIEEEE

Target ORF information:

RefSeq Version	XM_025141728.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus myosin, heavy chain 1G, skeletal muscle (MYH1G), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025141728.1

ORF Insert Sequence:

ATGGCTTCTT CAGATGCTGA GATGGCTGTC TTTGGAGAGG CAGCTCCCTA CCTCCGAAAG 
TCAGAGAAGG AAAGAATCGA GGCCCAGAAC AAGCCTTTTG ATGCCAAGTC ATCAGTCTTT 
GTGGCCCATC CAAAGGAATC CTTTGTGAAA GGGACAATCC AGAGCAGGGA AACAGGGAAG 
GTCACTGTCA AGACTGAAGG TGGAGAGACT CTGACTGTGA AGGAAGATCA GGTCTTCTCC 
ATGAACCCTC CCAAGTACGA TAAAATTGAG GACATGGCCA TGATGACCCA CCTCCATGAA 
CCCGCTGTGC TGTACAACCT CAAAGAGCGT TATGCAGCCT GGATGATCTA CACCTACTCG 
GGCCTCTTCT GTGTCACTGT CAACCCCTAC AAGTGGCTGC CGGTGTACAA CCCGGAGGTG 
GTGTTGGCCT ACCGAGGCAA AAAGCGCCAG GAGGCCCCTC CACACATCTT CTCCATCTCT 
GACAACGCCT ATCAGTTCAT GCTGACTGAC CGTGAGAATC AGTCGATCCT GATCACCGGA 
GAATCCGGTG CAGGGAAGAC TGTGAACACG AAACGTGTCA TCCAGTACTT TGCAACAATT 
GCAGCTAGTG GGGAGAAGAA GAAGGAGGAG CAGTCTGGCA AAATGCAGGG AACACTTGAG 
GATCAAATCA TCAGTGCCAA CCCCCTGCTG GAGGCCTTTG GAAATGCCAA GACTGTGAGG 
AATGACAACT CCTCACGCTT TGGTAAATTC ATCAGAATCC ATTTTGGGGC CACAGGCAAA 
CTGGCTTCTG CTGACATTGA AACATATCTG CTGGAAAAGT CCAGGGTTAC TTTCCAGCTC 
AAGGCAGAAA GAAGCTACCA CATCTTTTAT CAGGTCACCT CCAACAAGAA GCCAGAGCTA 
ATAGAAATGC TCCTTATCAC CACCAACCCA TATGACTACC CTTTTGTGAG TCAAGGTGAG 
ATCACTGTCC CCAGCATTGA TGACAAAGAG GAGCTTATGG CTACAGATAG TGCCATTGAC 
ATCCTGGGCT TCACTGCTGA TGAAAAGACT GCCATCTACA AGCTGACAGG GGCTGTCATG 
CACTACGGGA ATTTGAAGTT CAAGCAGAAA CAGCGAGAGG AGCAGGCAGA GCCAGATGGC 
ACAGAAGTGG CTGACAAGGC TGCCTACCTG ATGGGCCTGA ATTCAGCTGA CATGCTCAAG 
GCACTATGCT ACCCTCGAGT CAAAGTTGGG AATGAATATG TGACCAAGGG TCAGACTGTA 
CAACAGGTGC ACAATGCTGT AGGTGCTCTG GCAAAGGCTG TCTATGAGAG GATGTTCTTG 
TGGATGGTTG TTCGCATCAA CCAGCAGCTG GATACCAAGC AGCCCAGGCA GTACTTCATT 
GGTGTCCTGG ACATCGCTGG CTTTGAGATC TTTGATTTCA ACAGCTTCGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT TGTGCTGGAG 
CAAGAGGAGT ACAAAAAGGA AGGCATTGAA TGGACATTCA TTGACTTTGG GATGGACCTG 
GCTGCCTGCA TTGAGCTCAT TGAGAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCAAC CGACACCTCT TTCAAGAACA AGCTCTATGA CCAGCATCTG 
GGCAAGTCCA GCAACTTCCA GAAGCCCAAG CCTACCAAAG GAAAGGTTGA GGCCCACTTC 
TCCCTAATAC ACTATGCTGG CACAGTGGAC TACAATATCA CTGGCTGGCT TGAGAAGAAC 
AAGGACCCCC TGAATGAAAC TGTCATTGGG CTGTACCAGA AATCTTCACT GAAAACTTTG 
GCCTTACTGT TTGCCAACTA TGGTGGAGCA GAAGCAGAGG CTAGTGCTGG TGCTGGCAAG 
AAAGGTGGCA AGAAGAAAGG TTCTTCTTTC CAGACTGTCT CAGCTCTTTT CCGGGAGAAC 
TTAAACAAGC TGATGACCAA TCTGAGGAGC ACCCACCCCC ATTTTGTACG CTGTATCATC 
CCAAATGAAA CAAAAACACC TGGTGCCATG GAGCATGAGC TGGTTCTGCA CCAACTGAGG 
TGTAATGGTG TGCTGGAAGG GATCAGAATT TGCAGGAAAG GATTTCCCAA CAGAGTCCTG 
TATGCAGATT TTAAACAGAG ATACAAAGTA CTAAATGCAA GCGCTATTCC AGAGGGACAG 
TTCATTGACA GCAAGAAGGC TTGTGAGAAA CTGCTTGGAT CAATTGATAT AGACCACACT 
CAGTATAAAT TTGGTCACAC CAAGGTGTTC TTCAAAGCTG GGCTGGTTGG CCTTTTGGAG 
GAGATGAGGG ATGAGAAGCT GGCACAGCTC ATCACTCGCA CACAGGCCAG GTGCAGAGGC 
TTCCTGATGA GAGTGGAATA CCAGAAAATG GTGGAGAGGA GAGAGTCTGT CTTCTGCATC 
CAGTACAACA TTCGTGCATT CATGAATGTC AAGCACTGGC CCTGGATGAA GCTGTTCTTT 
AAGATCAAGC CGCTGCTGAA GAGCGCAGAG TCTGAGAAGG AGATGGCCAA CATGAAACAA 
GAGTTTGAGA AGACCAAGGA AGAGCTTGCA AAGTCTGAGG CAAAGAGGAA GGAGCTGGAG 
GAGAAAATGG TGAAGCTGGT GCAGGAGAAA AATGATCTGC AGCTCCAAGT GCAGGCTGAA 
GCTGATGCAT TGGCTGATGC TGAGGAAAGA TGTGACCAGC TCATCAAAAC CAAAATCCAG 
CTGGAAGCCA AAGTAAAGGA GGTGACAGAA CGGGCTGAGG ATGAGGAGGA AATTAATGCT 
GAACTGACAG CCAAGAAGAG AAAACTGGAG GATGAATGTT CAGAACTGAA GAAAGACATA 
GATGACCTGG AGTTAACATT GGCCAAAGTT GAGAAGGAAA AGCATGCCAC TGAAAACAAG 
GTGAAAAACC TCACAGAGGA GATGGCAGCC CTGGATGAGA CCATTGTGAA GCTGACAAAA 
GAGAAGAAAG CCCTCCAAGA GGCCCATCAG CAGACACTGG ATGACCTGCA GGCTGAAGAG 
GACAAAGTCA ATACGCTGAC CAAAGCTAAA ACCAAGCTGG AACAGCAAGT GGATGATCTG 
GAAGGGTCCC TGGAGCAAGA GAAGAAGCTG CGCATGGACC TCGAGAGAGC CAAGAGGAAA 
CTTGAAGGAG ACCTGAAGTT GGCCCATGAT AGCATAATGG ACTTGGAAAA TGATAAACAG 
CAGCTGGATG AGAAACTGAA GAAGAAAGAC TTTGAAATCA GCCAGATCCA GAGCAAAATT 
GAGGATGAAC AACTTCTGGG CATGCAGTTA CAGAAGAAGA TCAAGGAGCT GCAGGCCCGT 
ATTGAAGAAC TGGAGGAGGA AATTGAGGCA GAGCGAACCT CTCGGGCAAA AGCAGAGAAG 
CACCGAGCTG ACCTCTCCAG GGAGCTGGAG GAGATCAGTG GGCGCCTGGA GGAAGCAGGA 
GGGGCTACAG CAGCTCAGAT CGAGATGAAC AAGAAGCGTG AGGCAGAATT TCAGAAGATG 
CGCCGTGACC TCGAAGAGGC CACGCTGCAG CACGAAGCCA CGGCTGCTGC CCTGCGGAAG 
AAGCATGCGG ACAGCACAGC GGAGCTTGGG GAGCAGATTG ACAACCTGCA ACGAGTCAAG 
CAGAAGCTGG AGAAGGAGAA GAGTGAGCTG AAGATGGAGA TTGATGACTT GGCCAGCAAC 
ATGGAGTCTG TCTCCAAAGC TAAGGCAAAC CTGGAGAAGA TGTGTCGCAC TTTGGAAGAC 
CAACTAAGTG AATTCAAAAC GAAAGATGAG CAGAACCAGC GCATGATCAG TGATCTCAGT 
GCTCAAAGAG CTCGCCTGCA AACAGAATCT GGTGAATACG CACGCCAGGC GGACGAAAAA 
GATGGTTTAA TTTCTCAGCT GTCCAGAGGG AAACAGGCCT TCACGCAACA GATTGAAGAA 
CTAAAGCGGC AACTAGAAGA AGAGATAAAG GCCAAGAATG CCCTGGCCCA CAGCTTGCAG 
TCAGCCCGCC ATGACTGTGA TTTGCTCCGG GAACAATATG AGGAGGAGCT TGAAGCCAAG 
GGTGAGCTGC AGCGTGCCCT GTCCAAGGCC AACAGCGAAG TGGCCCAGTG GAGAACCAAA 
TATGAGACGG ATGCTATTCA GCGCACAGAG GAGCTGGAGG AGGCCAAGAA GAAGCTGGCA 
CAGCGCCTGC AGGATGCCGA GGAACATGTT GAAGCTGTGA ATGCCAAATG CGCTTCCCTG 
GAAAAGACAA AGCAGAGGCT GCAGAATGAG GTGGAGGACC TGATGATTGA CGTGGAGCGA 
GCAAATGCTG CCTGTGCAGC TCTGGACAAG AAGCAGAAGA ACTTTGACAA GATCCTGGCA 
GAGTGGAAGC AGAAGTACGA GGAAACGCAG GCTGAGCTGG AGGCCTCCCA GAAGGAGTCT 
CGCTCCCTCA GCACAGAGCT GTTTAAGATG AAGAATGCCT ATGAGGAGTC CCTGGACCAC 
CTGGAAACGC TGAAGCGTGA GAACAAGAAC TTGCAGCAGG AGATTTCTGA CCTCACAGAG 
CAGATTGCAG AGGGAGGAAA GGCGATTCAT GAGTTGGAGA AAGTCAAGAA GCAGATTGAG 
CAGGAGAAGT CTGAAATCCA GGCAGCACTG GAGGAAGCTG AGGCCTCCCT GGAACATGAA 
GAGGGGAAGA TCCTGCGCCT CCAACTGGAG CTCAACCAGG TCAAGTCTGA GATCGACAGG 
AAGATAGCAG AGAAAGATGA GGAAATTGAC CAGCTGAAGA GAAATCACCT CAGAATTGTG 
GAGTCCTTGC AGAGCAGCCT GGATGCTGAG ATCAGGAGCA GGAATGAGGC CCTGAGGCTG 
AAGAAGAAGA TGGAGGGAGA CCTGAATGAA ATGGAGATCC AGCTGAGCCA CGCTAACCGT 
GTTGCTGCAG AGGCACAAAA GAACCTGAGA AACACACAGG CAGTGTTGAA GGATACCCAG 
ATACATTTGG ACGACGCTCT CAGGACACAG GAGGACCTGA AGGAGCAGGT GGCCATGGTG 
GAGCGCAGAG CAAACCTGTT GCAGGCTGAG ATTGAGGAGC TACGGGCAGC CCTGGAGCAG 
ACAGAGCGCT CGAGAAAAGT GGCTGAGCAG GAACTGATGG ATGCAAGTGA GCGTGTGCAG 
CTCCTCCATA CCCAGAACAC CAGCTTGATC AACACCAAGA AGAAACTAGA AACGGACATT 
GCCCAAATTC AGAGTGAAAT GGAGGATACC ATCCAGGAAG CCCGCAATGC TGAAGAGAAG 
GCCAAGAAGG CCATCACCGA TGCAGCCATG ATGGCAGAAG AGCTGAAGAA GGAGCAGGAC 
ACCAGTGCCC ACCTGGAGAG GATGAAGAAG AACCTGGACC AGACGGTGAA GGACCTGCAG 
CACCGCCTGG ATGAGGCAGA GCAGCTGGCC CTGAAGGGAG GCAAGAAGCA AATTCAGAAG 
CTGGAGGCCA GGGTGCGGGA GCTGGAAGGG GAGGTTGATG CTGAGCAGAA GCGCAGCGCT 
GAAGCCGTGA AGGGTGTGCG CAAGTACGAG AGGAGGGTGA AGGAGCTGAC CTACCAGTCT 
GAGGAAGATC GCAAGAACAT TCTTAGACTC CAGGATCTGG TGGACAAACT ACAGATGAAG 
GTGAAATCCT ACAAGAGACA ATCTGAGGAG GCTGAGGAGC TGTCCAATGT CAACCTTTCC 
AAGTTCCGCA AGATCCAGCA CGAGCTGGAG GAGGCTGAGG AGCGGGCTGA CATTGCAGAG 
TCACAGGTCA ACAAGCTCCG AGCAAAGAGC CGGGAATTCC ATAGGAGGAT TGAAGAGGAA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH1G ( XM_025141729.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024997497.1 Gallus gallus myosin, heavy chain 1G, skeletal muscle (MYH1G), transcript variant X4, mRNA.

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CloneID	OGa16452
Clone ID Related Accession (Same CDS sequence)	XM_025141728.1 , XM_025141729.1
Accession Version	XM_025141729.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	myosin, heavy chain 1G, skeletal muscle isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006105.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTT CAGATGCTGA GATGGCTGTC TTTGGAGAGG CAGCTCCCTA CCTCCGAAAG 
TCAGAGAAGG AAAGAATCGA GGCCCAGAAC AAGCCTTTTG ATGCCAAGTC ATCAGTCTTT 
GTGGCCCATC CAAAGGAATC CTTTGTGAAA GGGACAATCC AGAGCAGGGA AACAGGGAAG 
GTCACTGTCA AGACTGAAGG TGGAGAGACT CTGACTGTGA AGGAAGATCA GGTCTTCTCC 
ATGAACCCTC CCAAGTACGA TAAAATTGAG GACATGGCCA TGATGACCCA CCTCCATGAA 
CCCGCTGTGC TGTACAACCT CAAAGAGCGT TATGCAGCCT GGATGATCTA CACCTACTCG 
GGCCTCTTCT GTGTCACTGT CAACCCCTAC AAGTGGCTGC CGGTGTACAA CCCGGAGGTG 
GTGTTGGCCT ACCGAGGCAA AAAGCGCCAG GAGGCCCCTC CACACATCTT CTCCATCTCT 
GACAACGCCT ATCAGTTCAT GCTGACTGAC CGTGAGAATC AGTCGATCCT GATCACCGGA 
GAATCCGGTG CAGGGAAGAC TGTGAACACG AAACGTGTCA TCCAGTACTT TGCAACAATT 
GCAGCTAGTG GGGAGAAGAA GAAGGAGGAG CAGTCTGGCA AAATGCAGGG AACACTTGAG 
GATCAAATCA TCAGTGCCAA CCCCCTGCTG GAGGCCTTTG GAAATGCCAA GACTGTGAGG 
AATGACAACT CCTCACGCTT TGGTAAATTC ATCAGAATCC ATTTTGGGGC CACAGGCAAA 
CTGGCTTCTG CTGACATTGA AACATATCTG CTGGAAAAGT CCAGGGTTAC TTTCCAGCTC 
AAGGCAGAAA GAAGCTACCA CATCTTTTAT CAGGTCACCT CCAACAAGAA GCCAGAGCTA 
ATAGAAATGC TCCTTATCAC CACCAACCCA TATGACTACC CTTTTGTGAG TCAAGGTGAG 
ATCACTGTCC CCAGCATTGA TGACAAAGAG GAGCTTATGG CTACAGATAG TGCCATTGAC 
ATCCTGGGCT TCACTGCTGA TGAAAAGACT GCCATCTACA AGCTGACAGG GGCTGTCATG 
CACTACGGGA ATTTGAAGTT CAAGCAGAAA CAGCGAGAGG AGCAGGCAGA GCCAGATGGC 
ACAGAAGTGG CTGACAAGGC TGCCTACCTG ATGGGCCTGA ATTCAGCTGA CATGCTCAAG 
GCACTATGCT ACCCTCGAGT CAAAGTTGGG AATGAATATG TGACCAAGGG TCAGACTGTA 
CAACAGGTGC ACAATGCTGT AGGTGCTCTG GCAAAGGCTG TCTATGAGAG GATGTTCTTG 
TGGATGGTTG TTCGCATCAA CCAGCAGCTG GATACCAAGC AGCCCAGGCA GTACTTCATT 
GGTGTCCTGG ACATCGCTGG CTTTGAGATC TTTGATTTCA ACAGCTTCGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT TGTGCTGGAG 
CAAGAGGAGT ACAAAAAGGA AGGCATTGAA TGGACATTCA TTGACTTTGG GATGGACCTG 
GCTGCCTGCA TTGAGCTCAT TGAGAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCAAC CGACACCTCT TTCAAGAACA AGCTCTATGA CCAGCATCTG 
GGCAAGTCCA GCAACTTCCA GAAGCCCAAG CCTACCAAAG GAAAGGTTGA GGCCCACTTC 
TCCCTAATAC ACTATGCTGG CACAGTGGAC TACAATATCA CTGGCTGGCT TGAGAAGAAC 
AAGGACCCCC TGAATGAAAC TGTCATTGGG CTGTACCAGA AATCTTCACT GAAAACTTTG 
GCCTTACTGT TTGCCAACTA TGGTGGAGCA GAAGCAGAGG CTAGTGCTGG TGCTGGCAAG 
AAAGGTGGCA AGAAGAAAGG TTCTTCTTTC CAGACTGTCT CAGCTCTTTT CCGGGAGAAC 
TTAAACAAGC TGATGACCAA TCTGAGGAGC ACCCACCCCC ATTTTGTACG CTGTATCATC 
CCAAATGAAA CAAAAACACC TGGTGCCATG GAGCATGAGC TGGTTCTGCA CCAACTGAGG 
TGTAATGGTG TGCTGGAAGG GATCAGAATT TGCAGGAAAG GATTTCCCAA CAGAGTCCTG 
TATGCAGATT TTAAACAGAG ATACAAAGTA CTAAATGCAA GCGCTATTCC AGAGGGACAG 
TTCATTGACA GCAAGAAGGC TTGTGAGAAA CTGCTTGGAT CAATTGATAT AGACCACACT 
CAGTATAAAT TTGGTCACAC CAAGGTGTTC TTCAAAGCTG GGCTGGTTGG CCTTTTGGAG 
GAGATGAGGG ATGAGAAGCT GGCACAGCTC ATCACTCGCA CACAGGCCAG GTGCAGAGGC 
TTCCTGATGA GAGTGGAATA CCAGAAAATG GTGGAGAGGA GAGAGTCTGT CTTCTGCATC 
CAGTACAACA TTCGTGCATT CATGAATGTC AAGCACTGGC CCTGGATGAA GCTGTTCTTT 
AAGATCAAGC CGCTGCTGAA GAGCGCAGAG TCTGAGAAGG AGATGGCCAA CATGAAACAA 
GAGTTTGAGA AGACCAAGGA AGAGCTTGCA AAGTCTGAGG CAAAGAGGAA GGAGCTGGAG 
GAGAAAATGG TGAAGCTGGT GCAGGAGAAA AATGATCTGC AGCTCCAAGT GCAGGCTGAA 
GCTGATGCAT TGGCTGATGC TGAGGAAAGA TGTGACCAGC TCATCAAAAC CAAAATCCAG 
CTGGAAGCCA AAGTAAAGGA GGTGACAGAA CGGGCTGAGG ATGAGGAGGA AATTAATGCT 
GAACTGACAG CCAAGAAGAG AAAACTGGAG GATGAATGTT CAGAACTGAA GAAAGACATA 
GATGACCTGG AGTTAACATT GGCCAAAGTT GAGAAGGAAA AGCATGCCAC TGAAAACAAG 
GTGAAAAACC TCACAGAGGA GATGGCAGCC CTGGATGAGA CCATTGTGAA GCTGACAAAA 
GAGAAGAAAG CCCTCCAAGA GGCCCATCAG CAGACACTGG ATGACCTGCA GGCTGAAGAG 
GACAAAGTCA ATACGCTGAC CAAAGCTAAA ACCAAGCTGG AACAGCAAGT GGATGATCTG 
GAAGGGTCCC TGGAGCAAGA GAAGAAGCTG CGCATGGACC TCGAGAGAGC CAAGAGGAAA 
CTTGAAGGAG ACCTGAAGTT GGCCCATGAT AGCATAATGG ACTTGGAAAA TGATAAACAG 
CAGCTGGATG AGAAACTGAA GAAGAAAGAC TTTGAAATCA GCCAGATCCA GAGCAAAATT 
GAGGATGAAC AACTTCTGGG CATGCAGTTA CAGAAGAAGA TCAAGGAGCT GCAGGCCCGT 
ATTGAAGAAC TGGAGGAGGA AATTGAGGCA GAGCGAACCT CTCGGGCAAA AGCAGAGAAG 
CACCGAGCTG ACCTCTCCAG GGAGCTGGAG GAGATCAGTG GGCGCCTGGA GGAAGCAGGA 
GGGGCTACAG CAGCTCAGAT CGAGATGAAC AAGAAGCGTG AGGCAGAATT TCAGAAGATG 
CGCCGTGACC TCGAAGAGGC CACGCTGCAG CACGAAGCCA CGGCTGCTGC CCTGCGGAAG 
AAGCATGCGG ACAGCACAGC GGAGCTTGGG GAGCAGATTG ACAACCTGCA ACGAGTCAAG 
CAGAAGCTGG AGAAGGAGAA GAGTGAGCTG AAGATGGAGA TTGATGACTT GGCCAGCAAC 
ATGGAGTCTG TCTCCAAAGC TAAGGCAAAC CTGGAGAAGA TGTGTCGCAC TTTGGAAGAC 
CAACTAAGTG AATTCAAAAC GAAAGATGAG CAGAACCAGC GCATGATCAG TGATCTCAGT 
GCTCAAAGAG CTCGCCTGCA AACAGAATCT GGTGAATACG CACGCCAGGC GGACGAAAAA 
GATGGTTTAA TTTCTCAGCT GTCCAGAGGG AAACAGGCCT TCACGCAACA GATTGAAGAA 
CTAAAGCGGC AACTAGAAGA AGAGATAAAG GCCAAGAATG CCCTGGCCCA CAGCTTGCAG 
TCAGCCCGCC ATGACTGTGA TTTGCTCCGG GAACAATATG AGGAGGAGCT TGAAGCCAAG 
GGTGAGCTGC AGCGTGCCCT GTCCAAGGCC AACAGCGAAG TGGCCCAGTG GAGAACCAAA 
TATGAGACGG ATGCTATTCA GCGCACAGAG GAGCTGGAGG AGGCCAAGAA GAAGCTGGCA 
CAGCGCCTGC AGGATGCCGA GGAACATGTT GAAGCTGTGA ATGCCAAATG CGCTTCCCTG 
GAAAAGACAA AGCAGAGGCT GCAGAATGAG GTGGAGGACC TGATGATTGA CGTGGAGCGA 
GCAAATGCTG CCTGTGCAGC TCTGGACAAG AAGCAGAAGA ACTTTGACAA GATCCTGGCA 
GAGTGGAAGC AGAAGTACGA GGAAACGCAG GCTGAGCTGG AGGCCTCCCA GAAGGAGTCT 
CGCTCCCTCA GCACAGAGCT GTTTAAGATG AAGAATGCCT ATGAGGAGTC CCTGGACCAC 
CTGGAAACGC TGAAGCGTGA GAACAAGAAC TTGCAGCAGG AGATTTCTGA CCTCACAGAG 
CAGATTGCAG AGGGAGGAAA GGCGATTCAT GAGTTGGAGA AAGTCAAGAA GCAGATTGAG 
CAGGAGAAGT CTGAAATCCA GGCAGCACTG GAGGAAGCTG AGGCCTCCCT GGAACATGAA 
GAGGGGAAGA TCCTGCGCCT CCAACTGGAG CTCAACCAGG TCAAGTCTGA GATCGACAGG 
AAGATAGCAG AGAAAGATGA GGAAATTGAC CAGCTGAAGA GAAATCACCT CAGAATTGTG 
GAGTCCTTGC AGAGCAGCCT GGATGCTGAG ATCAGGAGCA GGAATGAGGC CCTGAGGCTG 
AAGAAGAAGA TGGAGGGAGA CCTGAATGAA ATGGAGATCC AGCTGAGCCA CGCTAACCGT 
GTTGCTGCAG AGGCACAAAA GAACCTGAGA AACACACAGG CAGTGTTGAA GGATACCCAG 
ATACATTTGG ACGACGCTCT CAGGACACAG GAGGACCTGA AGGAGCAGGT GGCCATGGTG 
GAGCGCAGAG CAAACCTGTT GCAGGCTGAG ATTGAGGAGC TACGGGCAGC CCTGGAGCAG 
ACAGAGCGCT CGAGAAAAGT GGCTGAGCAG GAACTGATGG ATGCAAGTGA GCGTGTGCAG 
CTCCTCCATA CCCAGAACAC CAGCTTGATC AACACCAAGA AGAAACTAGA AACGGACATT 
GCCCAAATTC AGAGTGAAAT GGAGGATACC ATCCAGGAAG CCCGCAATGC TGAAGAGAAG 
GCCAAGAAGG CCATCACCGA TGCAGCCATG ATGGCAGAAG AGCTGAAGAA GGAGCAGGAC 
ACCAGTGCCC ACCTGGAGAG GATGAAGAAG AACCTGGACC AGACGGTGAA GGACCTGCAG 
CACCGCCTGG ATGAGGCAGA GCAGCTGGCC CTGAAGGGAG GCAAGAAGCA AATTCAGAAG 
CTGGAGGCCA GGGTGCGGGA GCTGGAAGGG GAGGTTGATG CTGAGCAGAA GCGCAGCGCT 
GAAGCCGTGA AGGGTGTGCG CAAGTACGAG AGGAGGGTGA AGGAGCTGAC CTACCAGTCT 
GAGGAAGATC GCAAGAACAT TCTTAGACTC CAGGATCTGG TGGACAAACT ACAGATGAAG 
GTGAAATCCT ACAAGAGACA ATCTGAGGAG GCTGAGGAGC TGTCCAATGT CAACCTTTCC 
AAGTTCCGCA AGATCCAGCA CGAGCTGGAG GAGGCTGAGG AGCGGGCTGA CATTGCAGAG 
TCACAGGTCA ACAAGCTCCG AGCAAAGAGC CGGGAATTCC ATAGGAGGAT TGAAGAGGAA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024997497.1
CDS	6076..11901
Translation	MASSDAEMAVFGEAAPYLRKSEKERIEAQNKPFDAKSSVFVAHPKESFVKGTIQSRETGKVTVKTEGGETLTVKEDQVFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVLAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAASGEKKKEEQSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLKAERSYHIFYQVTSNKKPELIEMLLITTNPYDYPFVSQGEITVPSIDDKEELMATDSAIDILGFTADEKTAIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLMGLNSADMLKALCYPRVKVGNEYVTKGQTVQQVHNAVGALAKAVYERMFLWMVVRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSSNFQKPKPTKGKVEAHFSLIHYAGTVDYNITGWLEKNKDPLNETVIGLYQKSSLKTLALLFANYGGAEAEASAGAGKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPNRVLYADFKQRYKVLNASAIPEGQFIDSKKACEKLLGSIDIDHTQYKFGHTKVFFKAGLVGLLEEMRDEKLAQLITRTQARCRGFLMRVEYQKMVERRESVFCIQYNIRAFMNVKHWPWMKLFFKIKPLLKSAESEKEMANMKQEFEKTKEELAKSEAKRKELEEKMVKLVQEKNDLQLQVQAEADALADAEERCDQLIKTKIQLEAKVKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAALDETIVKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAHDSIMDLENDKQQLDEKLKKKDFEISQIQSKIEDEQLLGMQLQKKIKELQARIEELEEEIEAERTSRAKAEKHRADLSRELEEISGRLEEAGGATAAQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSTAELGEQIDNLQRVKQKLEKEKSELKMEIDDLASNMESVSKAKANLEKMCRTLEDQLSEFKTKDEQNQRMISDLSAQRARLQTESGEYARQADEKDGLISQLSRGKQAFTQQIEELKRQLEEEIKAKNALAHSLQSARHDCDLLREQYEEELEAKGELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERANAACAALDKKQKNFDKILAEWKQKYEETQAELEASQKESRSLSTELFKMKNAYEESLDHLETLKRENKNLQQEISDLTEQIAEGGKAIHELEKVKKQIEQEKSEIQAALEEAEASLEHEEGKILRLQLELNQVKSEIDRKIAEKDEEIDQLKRNHLRIVESLQSSLDAEIRSRNEALRLKKKMEGDLNEMEIQLSHANRVAAEAQKNLRNTQAVLKDTQIHLDDALRTQEDLKEQVAMVERRANLLQAEIEELRAALEQTERSRKVAEQELMDASERVQLLHTQNTSLINTKKKLETDIAQIQSEMEDTIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLDQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVDAEQKRSAEAVKGVRKYERRVKELTYQSEEDRKNILRLQDLVDKLQMKVKSYKRQSEEAEELSNVNLSKFRKIQHELEEAEERADIAESQVNKLRAKSREFHRRIEEEE

Target ORF information:

RefSeq Version	XM_025141729.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus myosin, heavy chain 1G, skeletal muscle (MYH1G), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025141729.1

ORF Insert Sequence:

ATGGCTTCTT CAGATGCTGA GATGGCTGTC TTTGGAGAGG CAGCTCCCTA CCTCCGAAAG 
TCAGAGAAGG AAAGAATCGA GGCCCAGAAC AAGCCTTTTG ATGCCAAGTC ATCAGTCTTT 
GTGGCCCATC CAAAGGAATC CTTTGTGAAA GGGACAATCC AGAGCAGGGA AACAGGGAAG 
GTCACTGTCA AGACTGAAGG TGGAGAGACT CTGACTGTGA AGGAAGATCA GGTCTTCTCC 
ATGAACCCTC CCAAGTACGA TAAAATTGAG GACATGGCCA TGATGACCCA CCTCCATGAA 
CCCGCTGTGC TGTACAACCT CAAAGAGCGT TATGCAGCCT GGATGATCTA CACCTACTCG 
GGCCTCTTCT GTGTCACTGT CAACCCCTAC AAGTGGCTGC CGGTGTACAA CCCGGAGGTG 
GTGTTGGCCT ACCGAGGCAA AAAGCGCCAG GAGGCCCCTC CACACATCTT CTCCATCTCT 
GACAACGCCT ATCAGTTCAT GCTGACTGAC CGTGAGAATC AGTCGATCCT GATCACCGGA 
GAATCCGGTG CAGGGAAGAC TGTGAACACG AAACGTGTCA TCCAGTACTT TGCAACAATT 
GCAGCTAGTG GGGAGAAGAA GAAGGAGGAG CAGTCTGGCA AAATGCAGGG AACACTTGAG 
GATCAAATCA TCAGTGCCAA CCCCCTGCTG GAGGCCTTTG GAAATGCCAA GACTGTGAGG 
AATGACAACT CCTCACGCTT TGGTAAATTC ATCAGAATCC ATTTTGGGGC CACAGGCAAA 
CTGGCTTCTG CTGACATTGA AACATATCTG CTGGAAAAGT CCAGGGTTAC TTTCCAGCTC 
AAGGCAGAAA GAAGCTACCA CATCTTTTAT CAGGTCACCT CCAACAAGAA GCCAGAGCTA 
ATAGAAATGC TCCTTATCAC CACCAACCCA TATGACTACC CTTTTGTGAG TCAAGGTGAG 
ATCACTGTCC CCAGCATTGA TGACAAAGAG GAGCTTATGG CTACAGATAG TGCCATTGAC 
ATCCTGGGCT TCACTGCTGA TGAAAAGACT GCCATCTACA AGCTGACAGG GGCTGTCATG 
CACTACGGGA ATTTGAAGTT CAAGCAGAAA CAGCGAGAGG AGCAGGCAGA GCCAGATGGC 
ACAGAAGTGG CTGACAAGGC TGCCTACCTG ATGGGCCTGA ATTCAGCTGA CATGCTCAAG 
GCACTATGCT ACCCTCGAGT CAAAGTTGGG AATGAATATG TGACCAAGGG TCAGACTGTA 
CAACAGGTGC ACAATGCTGT AGGTGCTCTG GCAAAGGCTG TCTATGAGAG GATGTTCTTG 
TGGATGGTTG TTCGCATCAA CCAGCAGCTG GATACCAAGC AGCCCAGGCA GTACTTCATT 
GGTGTCCTGG ACATCGCTGG CTTTGAGATC TTTGATTTCA ACAGCTTCGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT TGTGCTGGAG 
CAAGAGGAGT ACAAAAAGGA AGGCATTGAA TGGACATTCA TTGACTTTGG GATGGACCTG 
GCTGCCTGCA TTGAGCTCAT TGAGAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCAAC CGACACCTCT TTCAAGAACA AGCTCTATGA CCAGCATCTG 
GGCAAGTCCA GCAACTTCCA GAAGCCCAAG CCTACCAAAG GAAAGGTTGA GGCCCACTTC 
TCCCTAATAC ACTATGCTGG CACAGTGGAC TACAATATCA CTGGCTGGCT TGAGAAGAAC 
AAGGACCCCC TGAATGAAAC TGTCATTGGG CTGTACCAGA AATCTTCACT GAAAACTTTG 
GCCTTACTGT TTGCCAACTA TGGTGGAGCA GAAGCAGAGG CTAGTGCTGG TGCTGGCAAG 
AAAGGTGGCA AGAAGAAAGG TTCTTCTTTC CAGACTGTCT CAGCTCTTTT CCGGGAGAAC 
TTAAACAAGC TGATGACCAA TCTGAGGAGC ACCCACCCCC ATTTTGTACG CTGTATCATC 
CCAAATGAAA CAAAAACACC TGGTGCCATG GAGCATGAGC TGGTTCTGCA CCAACTGAGG 
TGTAATGGTG TGCTGGAAGG GATCAGAATT TGCAGGAAAG GATTTCCCAA CAGAGTCCTG 
TATGCAGATT TTAAACAGAG ATACAAAGTA CTAAATGCAA GCGCTATTCC AGAGGGACAG 
TTCATTGACA GCAAGAAGGC TTGTGAGAAA CTGCTTGGAT CAATTGATAT AGACCACACT 
CAGTATAAAT TTGGTCACAC CAAGGTGTTC TTCAAAGCTG GGCTGGTTGG CCTTTTGGAG 
GAGATGAGGG ATGAGAAGCT GGCACAGCTC ATCACTCGCA CACAGGCCAG GTGCAGAGGC 
TTCCTGATGA GAGTGGAATA CCAGAAAATG GTGGAGAGGA GAGAGTCTGT CTTCTGCATC 
CAGTACAACA TTCGTGCATT CATGAATGTC AAGCACTGGC CCTGGATGAA GCTGTTCTTT 
AAGATCAAGC CGCTGCTGAA GAGCGCAGAG TCTGAGAAGG AGATGGCCAA CATGAAACAA 
GAGTTTGAGA AGACCAAGGA AGAGCTTGCA AAGTCTGAGG CAAAGAGGAA GGAGCTGGAG 
GAGAAAATGG TGAAGCTGGT GCAGGAGAAA AATGATCTGC AGCTCCAAGT GCAGGCTGAA 
GCTGATGCAT TGGCTGATGC TGAGGAAAGA TGTGACCAGC TCATCAAAAC CAAAATCCAG 
CTGGAAGCCA AAGTAAAGGA GGTGACAGAA CGGGCTGAGG ATGAGGAGGA AATTAATGCT 
GAACTGACAG CCAAGAAGAG AAAACTGGAG GATGAATGTT CAGAACTGAA GAAAGACATA 
GATGACCTGG AGTTAACATT GGCCAAAGTT GAGAAGGAAA AGCATGCCAC TGAAAACAAG 
GTGAAAAACC TCACAGAGGA GATGGCAGCC CTGGATGAGA CCATTGTGAA GCTGACAAAA 
GAGAAGAAAG CCCTCCAAGA GGCCCATCAG CAGACACTGG ATGACCTGCA GGCTGAAGAG 
GACAAAGTCA ATACGCTGAC CAAAGCTAAA ACCAAGCTGG AACAGCAAGT GGATGATCTG 
GAAGGGTCCC TGGAGCAAGA GAAGAAGCTG CGCATGGACC TCGAGAGAGC CAAGAGGAAA 
CTTGAAGGAG ACCTGAAGTT GGCCCATGAT AGCATAATGG ACTTGGAAAA TGATAAACAG 
CAGCTGGATG AGAAACTGAA GAAGAAAGAC TTTGAAATCA GCCAGATCCA GAGCAAAATT 
GAGGATGAAC AACTTCTGGG CATGCAGTTA CAGAAGAAGA TCAAGGAGCT GCAGGCCCGT 
ATTGAAGAAC TGGAGGAGGA AATTGAGGCA GAGCGAACCT CTCGGGCAAA AGCAGAGAAG 
CACCGAGCTG ACCTCTCCAG GGAGCTGGAG GAGATCAGTG GGCGCCTGGA GGAAGCAGGA 
GGGGCTACAG CAGCTCAGAT CGAGATGAAC AAGAAGCGTG AGGCAGAATT TCAGAAGATG 
CGCCGTGACC TCGAAGAGGC CACGCTGCAG CACGAAGCCA CGGCTGCTGC CCTGCGGAAG 
AAGCATGCGG ACAGCACAGC GGAGCTTGGG GAGCAGATTG ACAACCTGCA ACGAGTCAAG 
CAGAAGCTGG AGAAGGAGAA GAGTGAGCTG AAGATGGAGA TTGATGACTT GGCCAGCAAC 
ATGGAGTCTG TCTCCAAAGC TAAGGCAAAC CTGGAGAAGA TGTGTCGCAC TTTGGAAGAC 
CAACTAAGTG AATTCAAAAC GAAAGATGAG CAGAACCAGC GCATGATCAG TGATCTCAGT 
GCTCAAAGAG CTCGCCTGCA AACAGAATCT GGTGAATACG CACGCCAGGC GGACGAAAAA 
GATGGTTTAA TTTCTCAGCT GTCCAGAGGG AAACAGGCCT TCACGCAACA GATTGAAGAA 
CTAAAGCGGC AACTAGAAGA AGAGATAAAG GCCAAGAATG CCCTGGCCCA CAGCTTGCAG 
TCAGCCCGCC ATGACTGTGA TTTGCTCCGG GAACAATATG AGGAGGAGCT TGAAGCCAAG 
GGTGAGCTGC AGCGTGCCCT GTCCAAGGCC AACAGCGAAG TGGCCCAGTG GAGAACCAAA 
TATGAGACGG ATGCTATTCA GCGCACAGAG GAGCTGGAGG AGGCCAAGAA GAAGCTGGCA 
CAGCGCCTGC AGGATGCCGA GGAACATGTT GAAGCTGTGA ATGCCAAATG CGCTTCCCTG 
GAAAAGACAA AGCAGAGGCT GCAGAATGAG GTGGAGGACC TGATGATTGA CGTGGAGCGA 
GCAAATGCTG CCTGTGCAGC TCTGGACAAG AAGCAGAAGA ACTTTGACAA GATCCTGGCA 
GAGTGGAAGC AGAAGTACGA GGAAACGCAG GCTGAGCTGG AGGCCTCCCA GAAGGAGTCT 
CGCTCCCTCA GCACAGAGCT GTTTAAGATG AAGAATGCCT ATGAGGAGTC CCTGGACCAC 
CTGGAAACGC TGAAGCGTGA GAACAAGAAC TTGCAGCAGG AGATTTCTGA CCTCACAGAG 
CAGATTGCAG AGGGAGGAAA GGCGATTCAT GAGTTGGAGA AAGTCAAGAA GCAGATTGAG 
CAGGAGAAGT CTGAAATCCA GGCAGCACTG GAGGAAGCTG AGGCCTCCCT GGAACATGAA 
GAGGGGAAGA TCCTGCGCCT CCAACTGGAG CTCAACCAGG TCAAGTCTGA GATCGACAGG 
AAGATAGCAG AGAAAGATGA GGAAATTGAC CAGCTGAAGA GAAATCACCT CAGAATTGTG 
GAGTCCTTGC AGAGCAGCCT GGATGCTGAG ATCAGGAGCA GGAATGAGGC CCTGAGGCTG 
AAGAAGAAGA TGGAGGGAGA CCTGAATGAA ATGGAGATCC AGCTGAGCCA CGCTAACCGT 
GTTGCTGCAG AGGCACAAAA GAACCTGAGA AACACACAGG CAGTGTTGAA GGATACCCAG 
ATACATTTGG ACGACGCTCT CAGGACACAG GAGGACCTGA AGGAGCAGGT GGCCATGGTG 
GAGCGCAGAG CAAACCTGTT GCAGGCTGAG ATTGAGGAGC TACGGGCAGC CCTGGAGCAG 
ACAGAGCGCT CGAGAAAAGT GGCTGAGCAG GAACTGATGG ATGCAAGTGA GCGTGTGCAG 
CTCCTCCATA CCCAGAACAC CAGCTTGATC AACACCAAGA AGAAACTAGA AACGGACATT 
GCCCAAATTC AGAGTGAAAT GGAGGATACC ATCCAGGAAG CCCGCAATGC TGAAGAGAAG 
GCCAAGAAGG CCATCACCGA TGCAGCCATG ATGGCAGAAG AGCTGAAGAA GGAGCAGGAC 
ACCAGTGCCC ACCTGGAGAG GATGAAGAAG AACCTGGACC AGACGGTGAA GGACCTGCAG 
CACCGCCTGG ATGAGGCAGA GCAGCTGGCC CTGAAGGGAG GCAAGAAGCA AATTCAGAAG 
CTGGAGGCCA GGGTGCGGGA GCTGGAAGGG GAGGTTGATG CTGAGCAGAA GCGCAGCGCT 
GAAGCCGTGA AGGGTGTGCG CAAGTACGAG AGGAGGGTGA AGGAGCTGAC CTACCAGTCT 
GAGGAAGATC GCAAGAACAT TCTTAGACTC CAGGATCTGG TGGACAAACT ACAGATGAAG 
GTGAAATCCT ACAAGAGACA ATCTGAGGAG GCTGAGGAGC TGTCCAATGT CAACCTTTCC 
AAGTTCCGCA AGATCCAGCA CGAGCTGGAG GAGGCTGAGG AGCGGGCTGA CATTGCAGAG 
TCACAGGTCA ACAAGCTCCG AGCAAAGAGC CGGGAATTCC ATAGGAGGAT TGAAGAGGAA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH1G ( XM_015295221.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015150707.1 Gallus gallus myosin, heavy chain 1G, skeletal muscle (similar to human myosin, heavy chain 1, skeletal muscle, adult) (MYH1G), transcript variant X1, mRNA.

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CloneID	OGa43849
Clone ID Related Accession (Same CDS sequence)	XM_015295221.1 , XM_025141726.1 , XM_025141727.1
Accession Version	XM_015295221.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	myosin heavy chain, skeletal muscle, adult isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455986.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTT CAGATGCTGA GATGGCTGTC TTTGGAGAGG CAGCTCCCTA CCTCCGAAAG 
TCAGAGAAGG AAAGAATCGA GGCCCAGAAC AAGCCTTTTG ATGCCAAGTC ATCAGTCTTT 
GTGGCCCATC CAAAGGAATC CTTTGTGAAA GGGACAATCC AGAGCAGGGA AACAGGGAAG 
GTCACTGTCA AGACTGAAGG TGGAGAGACT CTGACTGTGA AGGAAGATCA GGTCTTCTCC 
ATGAACCCTC CCAAGTACGA TAAAATTGAG GACATGGCCA TGATGACCCA CCTCCATGAA 
CCCGCTGTGC TGTACAACCT CAAAGAGCGT TATGCAGCCT GGATGATCTA CACCTACTCG 
GGCCTCTTCT GTGTCACTGT CAACCCCTAC AAGTGGCTGC CGGTGTACAA CCCGGAGGTG 
GTGTTGGCCT ACCGAGGCAA AAAGCGCCAG GAGGCCCCTC CACACATCTT CTCCATCTCT 
GACAACGCCT ATCAGTTCAT GCTGACTGAC CGTGAGAATC AGTCGATCCT GATCACCGGA 
GAATCCGGTG CAGGGAAGAC TGTGAACACG AAACGTGTCA TCCAGTACTT TGCAACAATT 
GCAGCTAGTG GGGAGAAGAA GAAGGAGGAG CAGTCTGGCA AAATGCAGGG AACACTTGAG 
GATCAAATCA TCAGTGCCAA CCCCCTGCTG GAGGCCTTTG GAAATGCCAA GACTGTGAGG 
AATGACAACT CCTCACGCTT TGGTAAATTC ATCAGAATCC ATTTTGGGGC CACAGGCAAA 
CTGGCTTCTG CTGACATTGA AACATATCTG CTGGAAAAGT CCAGGGTTAC TTTCCAGCTC 
AAGGCAGAAA GAAGCTACCA CATCTTTTAT CAGGTCACCT CCAACAAGAA GCCAGAGCTA 
ATAGAAATGC TCCTTATCAC CACCAACCCA TATGACTACC CTTTTGTGAG TCAAGGTGAG 
ATCACTGTCC CCAGCATTGA TGACAAAGAG GAGCTTATGG CTACAGATAG TGCCATTGAC 
ATCCTGGGCT TCACTGCTGA TGAAAAGACT GCCATCTACA AGCTGACAGG GGCTGTCATG 
CACTACGGGA ATTTGAAGTT CAAGCAGAAA CAGCGAGAGG AGCAGGCAGA GCCAGATGGC 
ACAGAAGTGG CTGACAAGGC TGCCTACCTG ATGGGCCTGA ATTCAGCTGA CATGCTCAAG 
GCACTATGCT ACCCTCGAGT CAAAGTTGGG AATGAATATG TGACCAAGGG TCAGACTGTA 
CAACAGGTGC ACAATGCTGT AGGTGCTCTG GCAAAGGCTG TCTATGAGAG GATGTTCTTG 
TGGATGGTTG TTCGCATCAA CCAGCAGCTG GATACCAAGC AGCCCAGGCA GTACTTCATT 
GGTGTCCTGG ACATCGCTGG CTTTGAGATC TTTGATTTCA ACAGCTTCGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT TGTGCTGGAG 
CAAGAGGAGT ACAAAAAGGA AGGCATTGAA TGGACATTCA TTGACTTTGG GATGGACCTG 
GCTGCCTGCA TTGAGCTCAT TGAGAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCAAC CGACACCTCT TTCAAGAACA AGCTCTATGA CCAGCATCTG 
GGCAAGTCCA GCAACTTCCA GAAGCCCAAG CCTACCAAAG GAAAGGTTGA GGCCCACTTC 
TCCCTAATAC ACTATGCTGG CACAGTGGAC TACAATATCA CTGGCTGGCT TGAGAAGAAC 
AAGGACCCCC TGAATGAAAC TGTCATTGGG CTGTACCAGA AATCTTCACT GAAAACTTTG 
GCCTTACTGT TTGCCAACTA TGGTGGAGCA GAAGCAGAGG CTAGTGCTGG TGCTGGCAAG 
AAAGGTGGCA AGAAGAAAGG TTCTTCTTTC CAGACTGTCT CAGCTCTTTT CCGGGAGAAC 
TTAAACAAGC TGATGACCAA TCTGAGGAGC ACCCACCCCC ATTTTGTACG CTGTATCATC 
CCAAATGAAA CAAAAACACC TGGTAAGAGA CTCACCCACA GACAACCTTC AGTATCTCTT 
CTCTGCTGCG CTACAGAATA CAGCACTCAT TTATACTCTG AATTGCTAGG TGCCATGGAG 
CATGAGCTGG TTCTGCACCA ACTGAGGTGT AATGGTGTGC TGGAAGGGAT CAGAATTTGC 
AGGAAAGGAT TTCCCAACAG AGTCCTGTAT GCAGATTTTA AACAGAGATA CAAAGTACTA 
AATGCAAGCG CTATTCCAGA GGGACAGTTC ATTGACAGCA AGAAGGCTTG TGAGAAACTG 
CTTGGATCAA TTGATATAGA CCACACTCAG TATAAATTTG GTCACACCAA GGTGTTCTTC 
AAAGCTGGGC TGGTTGGCCT TTTGGAGGAG ATGAGGGATG AGAAGCTGGC ACAGCTCATC 
ACTCGCACAC AGGCCAGGTG CAGAGGCTTC CTGATGAGAG TGGAATACCA GAAAATGGTG 
GAGAGGAGAG AGTCTGTCTT CTGCATCCAG TACAACATTC GTGCATTCAT GAATGTCAAG 
CACTGGCCCT GGATGAAGCT GTTCTTTAAG ATCAAGCCGC TGCTGAAGAG CGCAGAGTCT 
GAGAAGGAGA TGGCCAACAT GAAACAAGAG TTTGAGAAGA CCAAGGAAGA GCTTGCAAAG 
TCTGAGGCAA AGAGGAAGGA GCTGGAGGAG AAAATGGTGA AGCTGGTGCA GGAGAAAAAT 
GATCTGCAGC TCCAAGTGCA GGCTGAAGCT GATGCATTGG CTGATGCTGA GGAAAGATGT 
GACCAGCTCA TCAAAACCAA AATCCAGCTG GAAGCCAAAG TAAAGGAGGT GACAGAACGG 
GCTGAGGATG AGGAGGAAAT TAATGCTGAA CTGACAGCCA AGAAGAGAAA ACTGGAGGAT 
GAATGTTCAG AACTGAAGAA AGACATAGAT GACCTGGAGT TAACATTGGC CAAAGTTGAG 
AAGGAAAAGC ATGCCACTGA AAACAAGGTG AAAAACCTCA CAGAGGAGAT GGCAGCCCTG 
GATGAGACCA TTGTGAAGCT GACAAAAGAG AAGAAAGCCC TCCAAGAGGC CCATCAGCAG 
ACACTGGATG ACCTGCAGGC TGAAGAGGAC AAAGTCAATA CGCTGACCAA AGCTAAAACC 
AAGCTGGAAC AGCAAGTGGA TGATCTGGAA GGGTCCCTGG AGCAAGAGAA GAAGCTGCGC 
ATGGACCTCG AGAGAGCCAA GAGGAAACTT GAAGGAGACC TGAAGTTGGC CCATGATAGC 
ATAATGGACT TGGAAAATGA TAAACAGCAG CTGGATGAGA AACTGAAGAA GAAAGACTTT 
GAAATCAGCC AGATCCAGAG CAAAATTGAG GATGAACAAC TTCTGGGCAT GCAGTTACAG 
AAGAAGATCA AGGAGCTGCA GGCCCGTATT GAAGAACTGG AGGAGGAAAT TGAGGCAGAG 
CGAACCTCTC GGGCAAAAGC AGAGAAGCAC CGAGCTGACC TCTCCAGGGA GCTGGAGGAG 
ATCAGTGGGC GCCTGGAGGA AGCAGGAGGG GCTACAGCAG CTCAGATCGA GATGAACAAG 
AAGCGTGAGG CAGAATTTCA GAAGATGCGC CGTGACCTCG AAGAGGCCAC GCTGCAGCAC 
GAAGCCACGG CTGCTGCCCT GCGGAAGAAG CATGCGGACA GCACAGCGGA GCTTGGGGAG 
CAGATTGACA ACCTGCAACG AGTCAAGCAG AAGCTGGAGA AGGAGAAGAG TGAGCTGAAG 
ATGGAGATTG ATGACTTGGC CAGCAACATG GAGTCTGTCT CCAAAGCTAA GGCAAACCTG 
GAGAAGATGT GTCGCACTTT GGAAGACCAA CTAAGTGAAT TCAAAACGAA AGATGAGCAG 
AACCAGCGCA TGATCAGTGA TCTCAGTGCT CAAAGAGCTC GCCTGCAAAC AGAATCTGGT 
GAATACGCAC GCCAGGCGGA CGAAAAAGAT GGTTTAATTT CTCAGCTGTC CAGAGGGAAA 
CAGGCCTTCA CGCAACAGAT TGAAGAACTA AAGCGGCAAC TAGAAGAAGA GATAAAGGCC 
AAGAATGCCC TGGCCCACAG CTTGCAGTCA GCCCGCCATG ACTGTGATTT GCTCCGGGAA 
CAATATGAGG AGGAGCTTGA AGCCAAGGGT GAGCTGCAGC GTGCCCTGTC CAAGGCCAAC 
AGCGAAGTGG CCCAGTGGAG AACCAAATAT GAGACGGATG CTATTCAGCG CACAGAGGAG 
CTGGAGGAGG CCAAGAAGAA GCTGGCACAG CGCCTGCAGG ATGCCGAGGA ACATGTTGAA 
GCTGTGAATG CCAAATGCGC TTCCCTGGAA AAGACAAAGC AGAGGCTGCA GAATGAGGTG 
GAGGACCTGA TGATTGACGT GGAGCGAGCA AATGCTGCCT GTGCAGCTCT GGACAAGAAG 
CAGAAGAACT TTGACAAGAT CCTGGCAGAG TGGAAGCAGA AGTACGAGGA AACGCAGGCT 
GAGCTGGAGG CCTCCCAGAA GGAGTCTCGC TCCCTCAGCA CAGAGCTGTT TAAGATGAAG 
AATGCCTATG AGGAGTCCCT GGACCACCTG GAAACGCTGA AGCGTGAGAA CAAGAACTTG 
CAGCAGGAGA TTTCTGACCT CACAGAGCAG ATTGCAGAGG GAGGAAAGGC GATTCATGAG 
TTGGAGAAAG TCAAGAAGCA GATTGAGCAG GAGAAGTCTG AAATCCAGGC AGCACTGGAG 
GAAGCTGAGG CCTCCCTGGA ACATGAAGAG GGGAAGATCC TGCGCCTCCA ACTGGAGCTC 
AACCAGGTCA AGTCTGAGAT CGACAGGAAG ATAGCAGAGA AAGATGAGGA AATTGACCAG 
CTGAAGAGAA ATCACCTCAG AATTGTGGAG TCCTTGCAGA GCAGCCTGGA TGCTGAGATC 
AGGAGCAGGA ATGAGGCCCT GAGGCTGAAG AAGAAGATGG AGGGAGACCT GAATGAAATG 
GAGATCCAGC TGAGCCACGC TAACCGTGTT GCTGCAGAGG CACAAAAGAA CCTGAGAAAC 
ACACAGGCAG TGTTGAAGGA TACCCAGATA CATTTGGACG ACGCTCTCAG GACACAGGAG 
GACCTGAAGG AGCAGGTGGC CATGGTGGAG CGCAGAGCAA ACCTGTTGCA GGCTGAGATT 
GAGGAGCTAC GGGCAGCCCT GGAGCAGACA GAGCGCTCGA GAAAAGTGGC TGAGCAGGAA 
CTGATGGATG CAAGTGAGCG TGTGCAGCTC CTCCATACCC AGAACACCAG CTTGATCAAC 
ACCAAGAAGA AACTAGAAAC GGACATTGCC CAAATTCAGA GTGAAATGGA GGATACCATC 
CAGGAAGCCC GCAATGCTGA AGAGAAGGCC AAGAAGGCCA TCACCGATGC AGCCATGATG 
GCAGAAGAGC TGAAGAAGGA GCAGGACACC AGTGCCCACC TGGAGAGGAT GAAGAAGAAC 
CTGGACCAGA CGGTGAAGGA CCTGCAGCAC CGCCTGGATG AGGCAGAGCA GCTGGCCCTG 
AAGGGAGGCA AGAAGCAAAT TCAGAAGCTG GAGGCCAGGG TGCGGGAGCT GGAAGGGGAG 
GTTGATGCTG AGCAGAAGCG CAGCGCTGAA GCCGTGAAGG GTGTGCGCAA GTACGAGAGG 
AGGGTGAAGG AGCTGACCTA CCAGTCTGAG GAAGATCGCA AGAACATTCT TAGACTCCAG 
GATCTGGTGG ACAAACTACA GATGAAGGTG AAATCCTACA AGAGACAATC TGAGGAGGCT 
GAGGAGCTGT CCAATGTCAA CCTTTCCAAG TTCCGCAAGA TCCAGCACGA GCTGGAGGAG 
GCTGAGGAGC GGGCTGACAT TGCAGAGTCA CAGGTCAACA AGCTCCGAGC AAAGAGCCGG 
GAATTCCATA GGAGGATTGA AGAGGAAGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015150707.1
CDS	16..5928
Translation	MASSDAEMAVFGEAAPYLRKSEKERIEAQNKPFDAKSSVFVAHPKESFVKGTIQSRETGKVTVKTEGGETLTVKEDQVFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVLAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAASGEKKKEEQSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLKAERSYHIFYQVTSNKKPELIEMLLITTNPYDYPFVSQGEITVPSIDDKEELMATDSAIDILGFTADEKTAIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLMGLNSADMLKALCYPRVKVGNEYVTKGQTVQQVHNAVGALAKAVYERMFLWMVVRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSSNFQKPKPTKGKVEAHFSLIHYAGTVDYNITGWLEKNKDPLNETVIGLYQKSSLKTLALLFANYGGAEAEASAGAGKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGKRLTHRQPSVSLLCCATEYSTHLYSELLGAMEHELVLHQLRCNGVLEGIRICRKGFPNRVLYADFKQRYKVLNASAIPEGQFIDSKKACEKLLGSIDIDHTQYKFGHTKVFFKAGLVGLLEEMRDEKLAQLITRTQARCRGFLMRVEYQKMVERRESVFCIQYNIRAFMNVKHWPWMKLFFKIKPLLKSAESEKEMANMKQEFEKTKEELAKSEAKRKELEEKMVKLVQEKNDLQLQVQAEADALADAEERCDQLIKTKIQLEAKVKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAALDETIVKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAHDSIMDLENDKQQLDEKLKKKDFEISQIQSKIEDEQLLGMQLQKKIKELQARIEELEEEIEAERTSRAKAEKHRADLSRELEEISGRLEEAGGATAAQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSTAELGEQIDNLQRVKQKLEKEKSELKMEIDDLASNMESVSKAKANLEKMCRTLEDQLSEFKTKDEQNQRMISDLSAQRARLQTESGEYARQADEKDGLISQLSRGKQAFTQQIEELKRQLEEEIKAKNALAHSLQSARHDCDLLREQYEEELEAKGELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERANAACAALDKKQKNFDKILAEWKQKYEETQAELEASQKESRSLSTELFKMKNAYEESLDHLETLKRENKNLQQEISDLTEQIAEGGKAIHELEKVKKQIEQEKSEIQAALEEAEASLEHEEGKILRLQLELNQVKSEIDRKIAEKDEEIDQLKRNHLRIVESLQSSLDAEIRSRNEALRLKKKMEGDLNEMEIQLSHANRVAAEAQKNLRNTQAVLKDTQIHLDDALRTQEDLKEQVAMVERRANLLQAEIEELRAALEQTERSRKVAEQELMDASERVQLLHTQNTSLINTKKKLETDIAQIQSEMEDTIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLDQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVDAEQKRSAEAVKGVRKYERRVKELTYQSEEDRKNILRLQDLVDKLQMKVKSYKRQSEEAEELSNVNLSKFRKIQHELEEAEERADIAESQVNKLRAKSREFHRRIEEEE

Target ORF information:

RefSeq Version	XM_015295221.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus myosin, heavy chain 1G, skeletal muscle (similar to human myosin, heavy chain 1, skeletal muscle, adult) (MYH1G), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015295221.1

ORF Insert Sequence:

ATGGCTTCTT CAGATGCTGA GATGGCTGTC TTTGGAGAGG CAGCTCCCTA CCTCCGAAAG 
TCAGAGAAGG AAAGAATCGA GGCCCAGAAC AAGCCTTTTG ATGCCAAGTC ATCAGTCTTT 
GTGGCCCATC CAAAGGAATC CTTTGTGAAA GGGACAATCC AGAGCAGGGA AACAGGGAAG 
GTCACTGTCA AGACTGAAGG TGGAGAGACT CTGACTGTGA AGGAAGATCA GGTCTTCTCC 
ATGAACCCTC CCAAGTACGA TAAAATTGAG GACATGGCCA TGATGACCCA CCTCCATGAA 
CCCGCTGTGC TGTACAACCT CAAAGAGCGT TATGCAGCCT GGATGATCTA CACCTACTCG 
GGCCTCTTCT GTGTCACTGT CAACCCCTAC AAGTGGCTGC CGGTGTACAA CCCGGAGGTG 
GTGTTGGCCT ACCGAGGCAA AAAGCGCCAG GAGGCCCCTC CACACATCTT CTCCATCTCT 
GACAACGCCT ATCAGTTCAT GCTGACTGAC CGTGAGAATC AGTCGATCCT GATCACCGGA 
GAATCCGGTG CAGGGAAGAC TGTGAACACG AAACGTGTCA TCCAGTACTT TGCAACAATT 
GCAGCTAGTG GGGAGAAGAA GAAGGAGGAG CAGTCTGGCA AAATGCAGGG AACACTTGAG 
GATCAAATCA TCAGTGCCAA CCCCCTGCTG GAGGCCTTTG GAAATGCCAA GACTGTGAGG 
AATGACAACT CCTCACGCTT TGGTAAATTC ATCAGAATCC ATTTTGGGGC CACAGGCAAA 
CTGGCTTCTG CTGACATTGA AACATATCTG CTGGAAAAGT CCAGGGTTAC TTTCCAGCTC 
AAGGCAGAAA GAAGCTACCA CATCTTTTAT CAGGTCACCT CCAACAAGAA GCCAGAGCTA 
ATAGAAATGC TCCTTATCAC CACCAACCCA TATGACTACC CTTTTGTGAG TCAAGGTGAG 
ATCACTGTCC CCAGCATTGA TGACAAAGAG GAGCTTATGG CTACAGATAG TGCCATTGAC 
ATCCTGGGCT TCACTGCTGA TGAAAAGACT GCCATCTACA AGCTGACAGG GGCTGTCATG 
CACTACGGGA ATTTGAAGTT CAAGCAGAAA CAGCGAGAGG AGCAGGCAGA GCCAGATGGC 
ACAGAAGTGG CTGACAAGGC TGCCTACCTG ATGGGCCTGA ATTCAGCTGA CATGCTCAAG 
GCACTATGCT ACCCTCGAGT CAAAGTTGGG AATGAATATG TGACCAAGGG TCAGACTGTA 
CAACAGGTGC ACAATGCTGT AGGTGCTCTG GCAAAGGCTG TCTATGAGAG GATGTTCTTG 
TGGATGGTTG TTCGCATCAA CCAGCAGCTG GATACCAAGC AGCCCAGGCA GTACTTCATT 
GGTGTCCTGG ACATCGCTGG CTTTGAGATC TTTGATTTCA ACAGCTTCGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT TGTGCTGGAG 
CAAGAGGAGT ACAAAAAGGA AGGCATTGAA TGGACATTCA TTGACTTTGG GATGGACCTG 
GCTGCCTGCA TTGAGCTCAT TGAGAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCAAC CGACACCTCT TTCAAGAACA AGCTCTATGA CCAGCATCTG 
GGCAAGTCCA GCAACTTCCA GAAGCCCAAG CCTACCAAAG GAAAGGTTGA GGCCCACTTC 
TCCCTAATAC ACTATGCTGG CACAGTGGAC TACAATATCA CTGGCTGGCT TGAGAAGAAC 
AAGGACCCCC TGAATGAAAC TGTCATTGGG CTGTACCAGA AATCTTCACT GAAAACTTTG 
GCCTTACTGT TTGCCAACTA TGGTGGAGCA GAAGCAGAGG CTAGTGCTGG TGCTGGCAAG 
AAAGGTGGCA AGAAGAAAGG TTCTTCTTTC CAGACTGTCT CAGCTCTTTT CCGGGAGAAC 
TTAAACAAGC TGATGACCAA TCTGAGGAGC ACCCACCCCC ATTTTGTACG CTGTATCATC 
CCAAATGAAA CAAAAACACC TGGTAAGAGA CTCACCCACA GACAACCTTC AGTATCTCTT 
CTCTGCTGCG CTACAGAATA CAGCACTCAT TTATACTCTG AATTGCTAGG TGCCATGGAG 
CATGAGCTGG TTCTGCACCA ACTGAGGTGT AATGGTGTGC TGGAAGGGAT CAGAATTTGC 
AGGAAAGGAT TTCCCAACAG AGTCCTGTAT GCAGATTTTA AACAGAGATA CAAAGTACTA 
AATGCAAGCG CTATTCCAGA GGGACAGTTC ATTGACAGCA AGAAGGCTTG TGAGAAACTG 
CTTGGATCAA TTGATATAGA CCACACTCAG TATAAATTTG GTCACACCAA GGTGTTCTTC 
AAAGCTGGGC TGGTTGGCCT TTTGGAGGAG ATGAGGGATG AGAAGCTGGC ACAGCTCATC 
ACTCGCACAC AGGCCAGGTG CAGAGGCTTC CTGATGAGAG TGGAATACCA GAAAATGGTG 
GAGAGGAGAG AGTCTGTCTT CTGCATCCAG TACAACATTC GTGCATTCAT GAATGTCAAG 
CACTGGCCCT GGATGAAGCT GTTCTTTAAG ATCAAGCCGC TGCTGAAGAG CGCAGAGTCT 
GAGAAGGAGA TGGCCAACAT GAAACAAGAG TTTGAGAAGA CCAAGGAAGA GCTTGCAAAG 
TCTGAGGCAA AGAGGAAGGA GCTGGAGGAG AAAATGGTGA AGCTGGTGCA GGAGAAAAAT 
GATCTGCAGC TCCAAGTGCA GGCTGAAGCT GATGCATTGG CTGATGCTGA GGAAAGATGT 
GACCAGCTCA TCAAAACCAA AATCCAGCTG GAAGCCAAAG TAAAGGAGGT GACAGAACGG 
GCTGAGGATG AGGAGGAAAT TAATGCTGAA CTGACAGCCA AGAAGAGAAA ACTGGAGGAT 
GAATGTTCAG AACTGAAGAA AGACATAGAT GACCTGGAGT TAACATTGGC CAAAGTTGAG 
AAGGAAAAGC ATGCCACTGA AAACAAGGTG AAAAACCTCA CAGAGGAGAT GGCAGCCCTG 
GATGAGACCA TTGTGAAGCT GACAAAAGAG AAGAAAGCCC TCCAAGAGGC CCATCAGCAG 
ACACTGGATG ACCTGCAGGC TGAAGAGGAC AAAGTCAATA CGCTGACCAA AGCTAAAACC 
AAGCTGGAAC AGCAAGTGGA TGATCTGGAA GGGTCCCTGG AGCAAGAGAA GAAGCTGCGC 
ATGGACCTCG AGAGAGCCAA GAGGAAACTT GAAGGAGACC TGAAGTTGGC CCATGATAGC 
ATAATGGACT TGGAAAATGA TAAACAGCAG CTGGATGAGA AACTGAAGAA GAAAGACTTT 
GAAATCAGCC AGATCCAGAG CAAAATTGAG GATGAACAAC TTCTGGGCAT GCAGTTACAG 
AAGAAGATCA AGGAGCTGCA GGCCCGTATT GAAGAACTGG AGGAGGAAAT TGAGGCAGAG 
CGAACCTCTC GGGCAAAAGC AGAGAAGCAC CGAGCTGACC TCTCCAGGGA GCTGGAGGAG 
ATCAGTGGGC GCCTGGAGGA AGCAGGAGGG GCTACAGCAG CTCAGATCGA GATGAACAAG 
AAGCGTGAGG CAGAATTTCA GAAGATGCGC CGTGACCTCG AAGAGGCCAC GCTGCAGCAC 
GAAGCCACGG CTGCTGCCCT GCGGAAGAAG CATGCGGACA GCACAGCGGA GCTTGGGGAG 
CAGATTGACA ACCTGCAACG AGTCAAGCAG AAGCTGGAGA AGGAGAAGAG TGAGCTGAAG 
ATGGAGATTG ATGACTTGGC CAGCAACATG GAGTCTGTCT CCAAAGCTAA GGCAAACCTG 
GAGAAGATGT GTCGCACTTT GGAAGACCAA CTAAGTGAAT TCAAAACGAA AGATGAGCAG 
AACCAGCGCA TGATCAGTGA TCTCAGTGCT CAAAGAGCTC GCCTGCAAAC AGAATCTGGT 
GAATACGCAC GCCAGGCGGA CGAAAAAGAT GGTTTAATTT CTCAGCTGTC CAGAGGGAAA 
CAGGCCTTCA CGCAACAGAT TGAAGAACTA AAGCGGCAAC TAGAAGAAGA GATAAAGGCC 
AAGAATGCCC TGGCCCACAG CTTGCAGTCA GCCCGCCATG ACTGTGATTT GCTCCGGGAA 
CAATATGAGG AGGAGCTTGA AGCCAAGGGT GAGCTGCAGC GTGCCCTGTC CAAGGCCAAC 
AGCGAAGTGG CCCAGTGGAG AACCAAATAT GAGACGGATG CTATTCAGCG CACAGAGGAG 
CTGGAGGAGG CCAAGAAGAA GCTGGCACAG CGCCTGCAGG ATGCCGAGGA ACATGTTGAA 
GCTGTGAATG CCAAATGCGC TTCCCTGGAA AAGACAAAGC AGAGGCTGCA GAATGAGGTG 
GAGGACCTGA TGATTGACGT GGAGCGAGCA AATGCTGCCT GTGCAGCTCT GGACAAGAAG 
CAGAAGAACT TTGACAAGAT CCTGGCAGAG TGGAAGCAGA AGTACGAGGA AACGCAGGCT 
GAGCTGGAGG CCTCCCAGAA GGAGTCTCGC TCCCTCAGCA CAGAGCTGTT TAAGATGAAG 
AATGCCTATG AGGAGTCCCT GGACCACCTG GAAACGCTGA AGCGTGAGAA CAAGAACTTG 
CAGCAGGAGA TTTCTGACCT CACAGAGCAG ATTGCAGAGG GAGGAAAGGC GATTCATGAG 
TTGGAGAAAG TCAAGAAGCA GATTGAGCAG GAGAAGTCTG AAATCCAGGC AGCACTGGAG 
GAAGCTGAGG CCTCCCTGGA ACATGAAGAG GGGAAGATCC TGCGCCTCCA ACTGGAGCTC 
AACCAGGTCA AGTCTGAGAT CGACAGGAAG ATAGCAGAGA AAGATGAGGA AATTGACCAG 
CTGAAGAGAA ATCACCTCAG AATTGTGGAG TCCTTGCAGA GCAGCCTGGA TGCTGAGATC 
AGGAGCAGGA ATGAGGCCCT GAGGCTGAAG AAGAAGATGG AGGGAGACCT GAATGAAATG 
GAGATCCAGC TGAGCCACGC TAACCGTGTT GCTGCAGAGG CACAAAAGAA CCTGAGAAAC 
ACACAGGCAG TGTTGAAGGA TACCCAGATA CATTTGGACG ACGCTCTCAG GACACAGGAG 
GACCTGAAGG AGCAGGTGGC CATGGTGGAG CGCAGAGCAA ACCTGTTGCA GGCTGAGATT 
GAGGAGCTAC GGGCAGCCCT GGAGCAGACA GAGCGCTCGA GAAAAGTGGC TGAGCAGGAA 
CTGATGGATG CAAGTGAGCG TGTGCAGCTC CTCCATACCC AGAACACCAG CTTGATCAAC 
ACCAAGAAGA AACTAGAAAC GGACATTGCC CAAATTCAGA GTGAAATGGA GGATACCATC 
CAGGAAGCCC GCAATGCTGA AGAGAAGGCC AAGAAGGCCA TCACCGATGC AGCCATGATG 
GCAGAAGAGC TGAAGAAGGA GCAGGACACC AGTGCCCACC TGGAGAGGAT GAAGAAGAAC 
CTGGACCAGA CGGTGAAGGA CCTGCAGCAC CGCCTGGATG AGGCAGAGCA GCTGGCCCTG 
AAGGGAGGCA AGAAGCAAAT TCAGAAGCTG GAGGCCAGGG TGCGGGAGCT GGAAGGGGAG 
GTTGATGCTG AGCAGAAGCG CAGCGCTGAA GCCGTGAAGG GTGTGCGCAA GTACGAGAGG 
AGGGTGAAGG AGCTGACCTA CCAGTCTGAG GAAGATCGCA AGAACATTCT TAGACTCCAG 
GATCTGGTGG ACAAACTACA GATGAAGGTG AAATCCTACA AGAGACAATC TGAGGAGGCT 
GAGGAGCTGT CCAATGTCAA CCTTTCCAAG TTCCGCAAGA TCCAGCACGA GCTGGAGGAG 
GCTGAGGAGC GGGCTGACAT TGCAGAGTCA CAGGTCAACA AGCTCCGAGC AAAGAGCCGG 
GAATTCCATA GGAGGATTGA AGAGGAAGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH1G ( XM_025141726.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024997494.1 Gallus gallus myosin, heavy chain 1G, skeletal muscle (MYH1G), transcript variant X1, mRNA.

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CloneID	OGa43849
Clone ID Related Accession (Same CDS sequence)	XM_015295221.1 , XM_025141726.1 , XM_025141727.1
Accession Version	XM_025141726.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	myosin, heavy chain 1G, skeletal muscle isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006105.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTT CAGATGCTGA GATGGCTGTC TTTGGAGAGG CAGCTCCCTA CCTCCGAAAG 
TCAGAGAAGG AAAGAATCGA GGCCCAGAAC AAGCCTTTTG ATGCCAAGTC ATCAGTCTTT 
GTGGCCCATC CAAAGGAATC CTTTGTGAAA GGGACAATCC AGAGCAGGGA AACAGGGAAG 
GTCACTGTCA AGACTGAAGG TGGAGAGACT CTGACTGTGA AGGAAGATCA GGTCTTCTCC 
ATGAACCCTC CCAAGTACGA TAAAATTGAG GACATGGCCA TGATGACCCA CCTCCATGAA 
CCCGCTGTGC TGTACAACCT CAAAGAGCGT TATGCAGCCT GGATGATCTA CACCTACTCG 
GGCCTCTTCT GTGTCACTGT CAACCCCTAC AAGTGGCTGC CGGTGTACAA CCCGGAGGTG 
GTGTTGGCCT ACCGAGGCAA AAAGCGCCAG GAGGCCCCTC CACACATCTT CTCCATCTCT 
GACAACGCCT ATCAGTTCAT GCTGACTGAC CGTGAGAATC AGTCGATCCT GATCACCGGA 
GAATCCGGTG CAGGGAAGAC TGTGAACACG AAACGTGTCA TCCAGTACTT TGCAACAATT 
GCAGCTAGTG GGGAGAAGAA GAAGGAGGAG CAGTCTGGCA AAATGCAGGG AACACTTGAG 
GATCAAATCA TCAGTGCCAA CCCCCTGCTG GAGGCCTTTG GAAATGCCAA GACTGTGAGG 
AATGACAACT CCTCACGCTT TGGTAAATTC ATCAGAATCC ATTTTGGGGC CACAGGCAAA 
CTGGCTTCTG CTGACATTGA AACATATCTG CTGGAAAAGT CCAGGGTTAC TTTCCAGCTC 
AAGGCAGAAA GAAGCTACCA CATCTTTTAT CAGGTCACCT CCAACAAGAA GCCAGAGCTA 
ATAGAAATGC TCCTTATCAC CACCAACCCA TATGACTACC CTTTTGTGAG TCAAGGTGAG 
ATCACTGTCC CCAGCATTGA TGACAAAGAG GAGCTTATGG CTACAGATAG TGCCATTGAC 
ATCCTGGGCT TCACTGCTGA TGAAAAGACT GCCATCTACA AGCTGACAGG GGCTGTCATG 
CACTACGGGA ATTTGAAGTT CAAGCAGAAA CAGCGAGAGG AGCAGGCAGA GCCAGATGGC 
ACAGAAGTGG CTGACAAGGC TGCCTACCTG ATGGGCCTGA ATTCAGCTGA CATGCTCAAG 
GCACTATGCT ACCCTCGAGT CAAAGTTGGG AATGAATATG TGACCAAGGG TCAGACTGTA 
CAACAGGTGC ACAATGCTGT AGGTGCTCTG GCAAAGGCTG TCTATGAGAG GATGTTCTTG 
TGGATGGTTG TTCGCATCAA CCAGCAGCTG GATACCAAGC AGCCCAGGCA GTACTTCATT 
GGTGTCCTGG ACATCGCTGG CTTTGAGATC TTTGATTTCA ACAGCTTCGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT TGTGCTGGAG 
CAAGAGGAGT ACAAAAAGGA AGGCATTGAA TGGACATTCA TTGACTTTGG GATGGACCTG 
GCTGCCTGCA TTGAGCTCAT TGAGAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCAAC CGACACCTCT TTCAAGAACA AGCTCTATGA CCAGCATCTG 
GGCAAGTCCA GCAACTTCCA GAAGCCCAAG CCTACCAAAG GAAAGGTTGA GGCCCACTTC 
TCCCTAATAC ACTATGCTGG CACAGTGGAC TACAATATCA CTGGCTGGCT TGAGAAGAAC 
AAGGACCCCC TGAATGAAAC TGTCATTGGG CTGTACCAGA AATCTTCACT GAAAACTTTG 
GCCTTACTGT TTGCCAACTA TGGTGGAGCA GAAGCAGAGG CTAGTGCTGG TGCTGGCAAG 
AAAGGTGGCA AGAAGAAAGG TTCTTCTTTC CAGACTGTCT CAGCTCTTTT CCGGGAGAAC 
TTAAACAAGC TGATGACCAA TCTGAGGAGC ACCCACCCCC ATTTTGTACG CTGTATCATC 
CCAAATGAAA CAAAAACACC TGGTAAGAGA CTCACCCACA GACAACCTTC AGTATCTCTT 
CTCTGCTGCG CTACAGAATA CAGCACTCAT TTATACTCTG AATTGCTAGG TGCCATGGAG 
CATGAGCTGG TTCTGCACCA ACTGAGGTGT AATGGTGTGC TGGAAGGGAT CAGAATTTGC 
AGGAAAGGAT TTCCCAACAG AGTCCTGTAT GCAGATTTTA AACAGAGATA CAAAGTACTA 
AATGCAAGCG CTATTCCAGA GGGACAGTTC ATTGACAGCA AGAAGGCTTG TGAGAAACTG 
CTTGGATCAA TTGATATAGA CCACACTCAG TATAAATTTG GTCACACCAA GGTGTTCTTC 
AAAGCTGGGC TGGTTGGCCT TTTGGAGGAG ATGAGGGATG AGAAGCTGGC ACAGCTCATC 
ACTCGCACAC AGGCCAGGTG CAGAGGCTTC CTGATGAGAG TGGAATACCA GAAAATGGTG 
GAGAGGAGAG AGTCTGTCTT CTGCATCCAG TACAACATTC GTGCATTCAT GAATGTCAAG 
CACTGGCCCT GGATGAAGCT GTTCTTTAAG ATCAAGCCGC TGCTGAAGAG CGCAGAGTCT 
GAGAAGGAGA TGGCCAACAT GAAACAAGAG TTTGAGAAGA CCAAGGAAGA GCTTGCAAAG 
TCTGAGGCAA AGAGGAAGGA GCTGGAGGAG AAAATGGTGA AGCTGGTGCA GGAGAAAAAT 
GATCTGCAGC TCCAAGTGCA GGCTGAAGCT GATGCATTGG CTGATGCTGA GGAAAGATGT 
GACCAGCTCA TCAAAACCAA AATCCAGCTG GAAGCCAAAG TAAAGGAGGT GACAGAACGG 
GCTGAGGATG AGGAGGAAAT TAATGCTGAA CTGACAGCCA AGAAGAGAAA ACTGGAGGAT 
GAATGTTCAG AACTGAAGAA AGACATAGAT GACCTGGAGT TAACATTGGC CAAAGTTGAG 
AAGGAAAAGC ATGCCACTGA AAACAAGGTG AAAAACCTCA CAGAGGAGAT GGCAGCCCTG 
GATGAGACCA TTGTGAAGCT GACAAAAGAG AAGAAAGCCC TCCAAGAGGC CCATCAGCAG 
ACACTGGATG ACCTGCAGGC TGAAGAGGAC AAAGTCAATA CGCTGACCAA AGCTAAAACC 
AAGCTGGAAC AGCAAGTGGA TGATCTGGAA GGGTCCCTGG AGCAAGAGAA GAAGCTGCGC 
ATGGACCTCG AGAGAGCCAA GAGGAAACTT GAAGGAGACC TGAAGTTGGC CCATGATAGC 
ATAATGGACT TGGAAAATGA TAAACAGCAG CTGGATGAGA AACTGAAGAA GAAAGACTTT 
GAAATCAGCC AGATCCAGAG CAAAATTGAG GATGAACAAC TTCTGGGCAT GCAGTTACAG 
AAGAAGATCA AGGAGCTGCA GGCCCGTATT GAAGAACTGG AGGAGGAAAT TGAGGCAGAG 
CGAACCTCTC GGGCAAAAGC AGAGAAGCAC CGAGCTGACC TCTCCAGGGA GCTGGAGGAG 
ATCAGTGGGC GCCTGGAGGA AGCAGGAGGG GCTACAGCAG CTCAGATCGA GATGAACAAG 
AAGCGTGAGG CAGAATTTCA GAAGATGCGC CGTGACCTCG AAGAGGCCAC GCTGCAGCAC 
GAAGCCACGG CTGCTGCCCT GCGGAAGAAG CATGCGGACA GCACAGCGGA GCTTGGGGAG 
CAGATTGACA ACCTGCAACG AGTCAAGCAG AAGCTGGAGA AGGAGAAGAG TGAGCTGAAG 
ATGGAGATTG ATGACTTGGC CAGCAACATG GAGTCTGTCT CCAAAGCTAA GGCAAACCTG 
GAGAAGATGT GTCGCACTTT GGAAGACCAA CTAAGTGAAT TCAAAACGAA AGATGAGCAG 
AACCAGCGCA TGATCAGTGA TCTCAGTGCT CAAAGAGCTC GCCTGCAAAC AGAATCTGGT 
GAATACGCAC GCCAGGCGGA CGAAAAAGAT GGTTTAATTT CTCAGCTGTC CAGAGGGAAA 
CAGGCCTTCA CGCAACAGAT TGAAGAACTA AAGCGGCAAC TAGAAGAAGA GATAAAGGCC 
AAGAATGCCC TGGCCCACAG CTTGCAGTCA GCCCGCCATG ACTGTGATTT GCTCCGGGAA 
CAATATGAGG AGGAGCTTGA AGCCAAGGGT GAGCTGCAGC GTGCCCTGTC CAAGGCCAAC 
AGCGAAGTGG CCCAGTGGAG AACCAAATAT GAGACGGATG CTATTCAGCG CACAGAGGAG 
CTGGAGGAGG CCAAGAAGAA GCTGGCACAG CGCCTGCAGG ATGCCGAGGA ACATGTTGAA 
GCTGTGAATG CCAAATGCGC TTCCCTGGAA AAGACAAAGC AGAGGCTGCA GAATGAGGTG 
GAGGACCTGA TGATTGACGT GGAGCGAGCA AATGCTGCCT GTGCAGCTCT GGACAAGAAG 
CAGAAGAACT TTGACAAGAT CCTGGCAGAG TGGAAGCAGA AGTACGAGGA AACGCAGGCT 
GAGCTGGAGG CCTCCCAGAA GGAGTCTCGC TCCCTCAGCA CAGAGCTGTT TAAGATGAAG 
AATGCCTATG AGGAGTCCCT GGACCACCTG GAAACGCTGA AGCGTGAGAA CAAGAACTTG 
CAGCAGGAGA TTTCTGACCT CACAGAGCAG ATTGCAGAGG GAGGAAAGGC GATTCATGAG 
TTGGAGAAAG TCAAGAAGCA GATTGAGCAG GAGAAGTCTG AAATCCAGGC AGCACTGGAG 
GAAGCTGAGG CCTCCCTGGA ACATGAAGAG GGGAAGATCC TGCGCCTCCA ACTGGAGCTC 
AACCAGGTCA AGTCTGAGAT CGACAGGAAG ATAGCAGAGA AAGATGAGGA AATTGACCAG 
CTGAAGAGAA ATCACCTCAG AATTGTGGAG TCCTTGCAGA GCAGCCTGGA TGCTGAGATC 
AGGAGCAGGA ATGAGGCCCT GAGGCTGAAG AAGAAGATGG AGGGAGACCT GAATGAAATG 
GAGATCCAGC TGAGCCACGC TAACCGTGTT GCTGCAGAGG CACAAAAGAA CCTGAGAAAC 
ACACAGGCAG TGTTGAAGGA TACCCAGATA CATTTGGACG ACGCTCTCAG GACACAGGAG 
GACCTGAAGG AGCAGGTGGC CATGGTGGAG CGCAGAGCAA ACCTGTTGCA GGCTGAGATT 
GAGGAGCTAC GGGCAGCCCT GGAGCAGACA GAGCGCTCGA GAAAAGTGGC TGAGCAGGAA 
CTGATGGATG CAAGTGAGCG TGTGCAGCTC CTCCATACCC AGAACACCAG CTTGATCAAC 
ACCAAGAAGA AACTAGAAAC GGACATTGCC CAAATTCAGA GTGAAATGGA GGATACCATC 
CAGGAAGCCC GCAATGCTGA AGAGAAGGCC AAGAAGGCCA TCACCGATGC AGCCATGATG 
GCAGAAGAGC TGAAGAAGGA GCAGGACACC AGTGCCCACC TGGAGAGGAT GAAGAAGAAC 
CTGGACCAGA CGGTGAAGGA CCTGCAGCAC CGCCTGGATG AGGCAGAGCA GCTGGCCCTG 
AAGGGAGGCA AGAAGCAAAT TCAGAAGCTG GAGGCCAGGG TGCGGGAGCT GGAAGGGGAG 
GTTGATGCTG AGCAGAAGCG CAGCGCTGAA GCCGTGAAGG GTGTGCGCAA GTACGAGAGG 
AGGGTGAAGG AGCTGACCTA CCAGTCTGAG GAAGATCGCA AGAACATTCT TAGACTCCAG 
GATCTGGTGG ACAAACTACA GATGAAGGTG AAATCCTACA AGAGACAATC TGAGGAGGCT 
GAGGAGCTGT CCAATGTCAA CCTTTCCAAG TTCCGCAAGA TCCAGCACGA GCTGGAGGAG 
GCTGAGGAGC GGGCTGACAT TGCAGAGTCA CAGGTCAACA AGCTCCGAGC AAAGAGCCGG 
GAATTCCATA GGAGGATTGA AGAGGAAGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024997494.1
CDS	6268..12180
Translation	MASSDAEMAVFGEAAPYLRKSEKERIEAQNKPFDAKSSVFVAHPKESFVKGTIQSRETGKVTVKTEGGETLTVKEDQVFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVLAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAASGEKKKEEQSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLKAERSYHIFYQVTSNKKPELIEMLLITTNPYDYPFVSQGEITVPSIDDKEELMATDSAIDILGFTADEKTAIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLMGLNSADMLKALCYPRVKVGNEYVTKGQTVQQVHNAVGALAKAVYERMFLWMVVRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSSNFQKPKPTKGKVEAHFSLIHYAGTVDYNITGWLEKNKDPLNETVIGLYQKSSLKTLALLFANYGGAEAEASAGAGKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGKRLTHRQPSVSLLCCATEYSTHLYSELLGAMEHELVLHQLRCNGVLEGIRICRKGFPNRVLYADFKQRYKVLNASAIPEGQFIDSKKACEKLLGSIDIDHTQYKFGHTKVFFKAGLVGLLEEMRDEKLAQLITRTQARCRGFLMRVEYQKMVERRESVFCIQYNIRAFMNVKHWPWMKLFFKIKPLLKSAESEKEMANMKQEFEKTKEELAKSEAKRKELEEKMVKLVQEKNDLQLQVQAEADALADAEERCDQLIKTKIQLEAKVKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAALDETIVKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAHDSIMDLENDKQQLDEKLKKKDFEISQIQSKIEDEQLLGMQLQKKIKELQARIEELEEEIEAERTSRAKAEKHRADLSRELEEISGRLEEAGGATAAQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSTAELGEQIDNLQRVKQKLEKEKSELKMEIDDLASNMESVSKAKANLEKMCRTLEDQLSEFKTKDEQNQRMISDLSAQRARLQTESGEYARQADEKDGLISQLSRGKQAFTQQIEELKRQLEEEIKAKNALAHSLQSARHDCDLLREQYEEELEAKGELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERANAACAALDKKQKNFDKILAEWKQKYEETQAELEASQKESRSLSTELFKMKNAYEESLDHLETLKRENKNLQQEISDLTEQIAEGGKAIHELEKVKKQIEQEKSEIQAALEEAEASLEHEEGKILRLQLELNQVKSEIDRKIAEKDEEIDQLKRNHLRIVESLQSSLDAEIRSRNEALRLKKKMEGDLNEMEIQLSHANRVAAEAQKNLRNTQAVLKDTQIHLDDALRTQEDLKEQVAMVERRANLLQAEIEELRAALEQTERSRKVAEQELMDASERVQLLHTQNTSLINTKKKLETDIAQIQSEMEDTIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLDQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVDAEQKRSAEAVKGVRKYERRVKELTYQSEEDRKNILRLQDLVDKLQMKVKSYKRQSEEAEELSNVNLSKFRKIQHELEEAEERADIAESQVNKLRAKSREFHRRIEEEE

Target ORF information:

RefSeq Version	XM_025141726.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus myosin, heavy chain 1G, skeletal muscle (MYH1G), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025141726.1

ORF Insert Sequence:

ATGGCTTCTT CAGATGCTGA GATGGCTGTC TTTGGAGAGG CAGCTCCCTA CCTCCGAAAG 
TCAGAGAAGG AAAGAATCGA GGCCCAGAAC AAGCCTTTTG ATGCCAAGTC ATCAGTCTTT 
GTGGCCCATC CAAAGGAATC CTTTGTGAAA GGGACAATCC AGAGCAGGGA AACAGGGAAG 
GTCACTGTCA AGACTGAAGG TGGAGAGACT CTGACTGTGA AGGAAGATCA GGTCTTCTCC 
ATGAACCCTC CCAAGTACGA TAAAATTGAG GACATGGCCA TGATGACCCA CCTCCATGAA 
CCCGCTGTGC TGTACAACCT CAAAGAGCGT TATGCAGCCT GGATGATCTA CACCTACTCG 
GGCCTCTTCT GTGTCACTGT CAACCCCTAC AAGTGGCTGC CGGTGTACAA CCCGGAGGTG 
GTGTTGGCCT ACCGAGGCAA AAAGCGCCAG GAGGCCCCTC CACACATCTT CTCCATCTCT 
GACAACGCCT ATCAGTTCAT GCTGACTGAC CGTGAGAATC AGTCGATCCT GATCACCGGA 
GAATCCGGTG CAGGGAAGAC TGTGAACACG AAACGTGTCA TCCAGTACTT TGCAACAATT 
GCAGCTAGTG GGGAGAAGAA GAAGGAGGAG CAGTCTGGCA AAATGCAGGG AACACTTGAG 
GATCAAATCA TCAGTGCCAA CCCCCTGCTG GAGGCCTTTG GAAATGCCAA GACTGTGAGG 
AATGACAACT CCTCACGCTT TGGTAAATTC ATCAGAATCC ATTTTGGGGC CACAGGCAAA 
CTGGCTTCTG CTGACATTGA AACATATCTG CTGGAAAAGT CCAGGGTTAC TTTCCAGCTC 
AAGGCAGAAA GAAGCTACCA CATCTTTTAT CAGGTCACCT CCAACAAGAA GCCAGAGCTA 
ATAGAAATGC TCCTTATCAC CACCAACCCA TATGACTACC CTTTTGTGAG TCAAGGTGAG 
ATCACTGTCC CCAGCATTGA TGACAAAGAG GAGCTTATGG CTACAGATAG TGCCATTGAC 
ATCCTGGGCT TCACTGCTGA TGAAAAGACT GCCATCTACA AGCTGACAGG GGCTGTCATG 
CACTACGGGA ATTTGAAGTT CAAGCAGAAA CAGCGAGAGG AGCAGGCAGA GCCAGATGGC 
ACAGAAGTGG CTGACAAGGC TGCCTACCTG ATGGGCCTGA ATTCAGCTGA CATGCTCAAG 
GCACTATGCT ACCCTCGAGT CAAAGTTGGG AATGAATATG TGACCAAGGG TCAGACTGTA 
CAACAGGTGC ACAATGCTGT AGGTGCTCTG GCAAAGGCTG TCTATGAGAG GATGTTCTTG 
TGGATGGTTG TTCGCATCAA CCAGCAGCTG GATACCAAGC AGCCCAGGCA GTACTTCATT 
GGTGTCCTGG ACATCGCTGG CTTTGAGATC TTTGATTTCA ACAGCTTCGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT TGTGCTGGAG 
CAAGAGGAGT ACAAAAAGGA AGGCATTGAA TGGACATTCA TTGACTTTGG GATGGACCTG 
GCTGCCTGCA TTGAGCTCAT TGAGAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCAAC CGACACCTCT TTCAAGAACA AGCTCTATGA CCAGCATCTG 
GGCAAGTCCA GCAACTTCCA GAAGCCCAAG CCTACCAAAG GAAAGGTTGA GGCCCACTTC 
TCCCTAATAC ACTATGCTGG CACAGTGGAC TACAATATCA CTGGCTGGCT TGAGAAGAAC 
AAGGACCCCC TGAATGAAAC TGTCATTGGG CTGTACCAGA AATCTTCACT GAAAACTTTG 
GCCTTACTGT TTGCCAACTA TGGTGGAGCA GAAGCAGAGG CTAGTGCTGG TGCTGGCAAG 
AAAGGTGGCA AGAAGAAAGG TTCTTCTTTC CAGACTGTCT CAGCTCTTTT CCGGGAGAAC 
TTAAACAAGC TGATGACCAA TCTGAGGAGC ACCCACCCCC ATTTTGTACG CTGTATCATC 
CCAAATGAAA CAAAAACACC TGGTAAGAGA CTCACCCACA GACAACCTTC AGTATCTCTT 
CTCTGCTGCG CTACAGAATA CAGCACTCAT TTATACTCTG AATTGCTAGG TGCCATGGAG 
CATGAGCTGG TTCTGCACCA ACTGAGGTGT AATGGTGTGC TGGAAGGGAT CAGAATTTGC 
AGGAAAGGAT TTCCCAACAG AGTCCTGTAT GCAGATTTTA AACAGAGATA CAAAGTACTA 
AATGCAAGCG CTATTCCAGA GGGACAGTTC ATTGACAGCA AGAAGGCTTG TGAGAAACTG 
CTTGGATCAA TTGATATAGA CCACACTCAG TATAAATTTG GTCACACCAA GGTGTTCTTC 
AAAGCTGGGC TGGTTGGCCT TTTGGAGGAG ATGAGGGATG AGAAGCTGGC ACAGCTCATC 
ACTCGCACAC AGGCCAGGTG CAGAGGCTTC CTGATGAGAG TGGAATACCA GAAAATGGTG 
GAGAGGAGAG AGTCTGTCTT CTGCATCCAG TACAACATTC GTGCATTCAT GAATGTCAAG 
CACTGGCCCT GGATGAAGCT GTTCTTTAAG ATCAAGCCGC TGCTGAAGAG CGCAGAGTCT 
GAGAAGGAGA TGGCCAACAT GAAACAAGAG TTTGAGAAGA CCAAGGAAGA GCTTGCAAAG 
TCTGAGGCAA AGAGGAAGGA GCTGGAGGAG AAAATGGTGA AGCTGGTGCA GGAGAAAAAT 
GATCTGCAGC TCCAAGTGCA GGCTGAAGCT GATGCATTGG CTGATGCTGA GGAAAGATGT 
GACCAGCTCA TCAAAACCAA AATCCAGCTG GAAGCCAAAG TAAAGGAGGT GACAGAACGG 
GCTGAGGATG AGGAGGAAAT TAATGCTGAA CTGACAGCCA AGAAGAGAAA ACTGGAGGAT 
GAATGTTCAG AACTGAAGAA AGACATAGAT GACCTGGAGT TAACATTGGC CAAAGTTGAG 
AAGGAAAAGC ATGCCACTGA AAACAAGGTG AAAAACCTCA CAGAGGAGAT GGCAGCCCTG 
GATGAGACCA TTGTGAAGCT GACAAAAGAG AAGAAAGCCC TCCAAGAGGC CCATCAGCAG 
ACACTGGATG ACCTGCAGGC TGAAGAGGAC AAAGTCAATA CGCTGACCAA AGCTAAAACC 
AAGCTGGAAC AGCAAGTGGA TGATCTGGAA GGGTCCCTGG AGCAAGAGAA GAAGCTGCGC 
ATGGACCTCG AGAGAGCCAA GAGGAAACTT GAAGGAGACC TGAAGTTGGC CCATGATAGC 
ATAATGGACT TGGAAAATGA TAAACAGCAG CTGGATGAGA AACTGAAGAA GAAAGACTTT 
GAAATCAGCC AGATCCAGAG CAAAATTGAG GATGAACAAC TTCTGGGCAT GCAGTTACAG 
AAGAAGATCA AGGAGCTGCA GGCCCGTATT GAAGAACTGG AGGAGGAAAT TGAGGCAGAG 
CGAACCTCTC GGGCAAAAGC AGAGAAGCAC CGAGCTGACC TCTCCAGGGA GCTGGAGGAG 
ATCAGTGGGC GCCTGGAGGA AGCAGGAGGG GCTACAGCAG CTCAGATCGA GATGAACAAG 
AAGCGTGAGG CAGAATTTCA GAAGATGCGC CGTGACCTCG AAGAGGCCAC GCTGCAGCAC 
GAAGCCACGG CTGCTGCCCT GCGGAAGAAG CATGCGGACA GCACAGCGGA GCTTGGGGAG 
CAGATTGACA ACCTGCAACG AGTCAAGCAG AAGCTGGAGA AGGAGAAGAG TGAGCTGAAG 
ATGGAGATTG ATGACTTGGC CAGCAACATG GAGTCTGTCT CCAAAGCTAA GGCAAACCTG 
GAGAAGATGT GTCGCACTTT GGAAGACCAA CTAAGTGAAT TCAAAACGAA AGATGAGCAG 
AACCAGCGCA TGATCAGTGA TCTCAGTGCT CAAAGAGCTC GCCTGCAAAC AGAATCTGGT 
GAATACGCAC GCCAGGCGGA CGAAAAAGAT GGTTTAATTT CTCAGCTGTC CAGAGGGAAA 
CAGGCCTTCA CGCAACAGAT TGAAGAACTA AAGCGGCAAC TAGAAGAAGA GATAAAGGCC 
AAGAATGCCC TGGCCCACAG CTTGCAGTCA GCCCGCCATG ACTGTGATTT GCTCCGGGAA 
CAATATGAGG AGGAGCTTGA AGCCAAGGGT GAGCTGCAGC GTGCCCTGTC CAAGGCCAAC 
AGCGAAGTGG CCCAGTGGAG AACCAAATAT GAGACGGATG CTATTCAGCG CACAGAGGAG 
CTGGAGGAGG CCAAGAAGAA GCTGGCACAG CGCCTGCAGG ATGCCGAGGA ACATGTTGAA 
GCTGTGAATG CCAAATGCGC TTCCCTGGAA AAGACAAAGC AGAGGCTGCA GAATGAGGTG 
GAGGACCTGA TGATTGACGT GGAGCGAGCA AATGCTGCCT GTGCAGCTCT GGACAAGAAG 
CAGAAGAACT TTGACAAGAT CCTGGCAGAG TGGAAGCAGA AGTACGAGGA AACGCAGGCT 
GAGCTGGAGG CCTCCCAGAA GGAGTCTCGC TCCCTCAGCA CAGAGCTGTT TAAGATGAAG 
AATGCCTATG AGGAGTCCCT GGACCACCTG GAAACGCTGA AGCGTGAGAA CAAGAACTTG 
CAGCAGGAGA TTTCTGACCT CACAGAGCAG ATTGCAGAGG GAGGAAAGGC GATTCATGAG 
TTGGAGAAAG TCAAGAAGCA GATTGAGCAG GAGAAGTCTG AAATCCAGGC AGCACTGGAG 
GAAGCTGAGG CCTCCCTGGA ACATGAAGAG GGGAAGATCC TGCGCCTCCA ACTGGAGCTC 
AACCAGGTCA AGTCTGAGAT CGACAGGAAG ATAGCAGAGA AAGATGAGGA AATTGACCAG 
CTGAAGAGAA ATCACCTCAG AATTGTGGAG TCCTTGCAGA GCAGCCTGGA TGCTGAGATC 
AGGAGCAGGA ATGAGGCCCT GAGGCTGAAG AAGAAGATGG AGGGAGACCT GAATGAAATG 
GAGATCCAGC TGAGCCACGC TAACCGTGTT GCTGCAGAGG CACAAAAGAA CCTGAGAAAC 
ACACAGGCAG TGTTGAAGGA TACCCAGATA CATTTGGACG ACGCTCTCAG GACACAGGAG 
GACCTGAAGG AGCAGGTGGC CATGGTGGAG CGCAGAGCAA ACCTGTTGCA GGCTGAGATT 
GAGGAGCTAC GGGCAGCCCT GGAGCAGACA GAGCGCTCGA GAAAAGTGGC TGAGCAGGAA 
CTGATGGATG CAAGTGAGCG TGTGCAGCTC CTCCATACCC AGAACACCAG CTTGATCAAC 
ACCAAGAAGA AACTAGAAAC GGACATTGCC CAAATTCAGA GTGAAATGGA GGATACCATC 
CAGGAAGCCC GCAATGCTGA AGAGAAGGCC AAGAAGGCCA TCACCGATGC AGCCATGATG 
GCAGAAGAGC TGAAGAAGGA GCAGGACACC AGTGCCCACC TGGAGAGGAT GAAGAAGAAC 
CTGGACCAGA CGGTGAAGGA CCTGCAGCAC CGCCTGGATG AGGCAGAGCA GCTGGCCCTG 
AAGGGAGGCA AGAAGCAAAT TCAGAAGCTG GAGGCCAGGG TGCGGGAGCT GGAAGGGGAG 
GTTGATGCTG AGCAGAAGCG CAGCGCTGAA GCCGTGAAGG GTGTGCGCAA GTACGAGAGG 
AGGGTGAAGG AGCTGACCTA CCAGTCTGAG GAAGATCGCA AGAACATTCT TAGACTCCAG 
GATCTGGTGG ACAAACTACA GATGAAGGTG AAATCCTACA AGAGACAATC TGAGGAGGCT 
GAGGAGCTGT CCAATGTCAA CCTTTCCAAG TTCCGCAAGA TCCAGCACGA GCTGGAGGAG 
GCTGAGGAGC GGGCTGACAT TGCAGAGTCA CAGGTCAACA AGCTCCGAGC AAAGAGCCGG 
GAATTCCATA GGAGGATTGA AGAGGAAGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH1G ( XM_025141727.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024997495.1 Gallus gallus myosin, heavy chain 1G, skeletal muscle (MYH1G), transcript variant X2, mRNA.

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CloneID	OGa43849
Clone ID Related Accession (Same CDS sequence)	XM_015295221.1 , XM_025141726.1 , XM_025141727.1
Accession Version	XM_025141727.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	myosin, heavy chain 1G, skeletal muscle isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006105.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTT CAGATGCTGA GATGGCTGTC TTTGGAGAGG CAGCTCCCTA CCTCCGAAAG 
TCAGAGAAGG AAAGAATCGA GGCCCAGAAC AAGCCTTTTG ATGCCAAGTC ATCAGTCTTT 
GTGGCCCATC CAAAGGAATC CTTTGTGAAA GGGACAATCC AGAGCAGGGA AACAGGGAAG 
GTCACTGTCA AGACTGAAGG TGGAGAGACT CTGACTGTGA AGGAAGATCA GGTCTTCTCC 
ATGAACCCTC CCAAGTACGA TAAAATTGAG GACATGGCCA TGATGACCCA CCTCCATGAA 
CCCGCTGTGC TGTACAACCT CAAAGAGCGT TATGCAGCCT GGATGATCTA CACCTACTCG 
GGCCTCTTCT GTGTCACTGT CAACCCCTAC AAGTGGCTGC CGGTGTACAA CCCGGAGGTG 
GTGTTGGCCT ACCGAGGCAA AAAGCGCCAG GAGGCCCCTC CACACATCTT CTCCATCTCT 
GACAACGCCT ATCAGTTCAT GCTGACTGAC CGTGAGAATC AGTCGATCCT GATCACCGGA 
GAATCCGGTG CAGGGAAGAC TGTGAACACG AAACGTGTCA TCCAGTACTT TGCAACAATT 
GCAGCTAGTG GGGAGAAGAA GAAGGAGGAG CAGTCTGGCA AAATGCAGGG AACACTTGAG 
GATCAAATCA TCAGTGCCAA CCCCCTGCTG GAGGCCTTTG GAAATGCCAA GACTGTGAGG 
AATGACAACT CCTCACGCTT TGGTAAATTC ATCAGAATCC ATTTTGGGGC CACAGGCAAA 
CTGGCTTCTG CTGACATTGA AACATATCTG CTGGAAAAGT CCAGGGTTAC TTTCCAGCTC 
AAGGCAGAAA GAAGCTACCA CATCTTTTAT CAGGTCACCT CCAACAAGAA GCCAGAGCTA 
ATAGAAATGC TCCTTATCAC CACCAACCCA TATGACTACC CTTTTGTGAG TCAAGGTGAG 
ATCACTGTCC CCAGCATTGA TGACAAAGAG GAGCTTATGG CTACAGATAG TGCCATTGAC 
ATCCTGGGCT TCACTGCTGA TGAAAAGACT GCCATCTACA AGCTGACAGG GGCTGTCATG 
CACTACGGGA ATTTGAAGTT CAAGCAGAAA CAGCGAGAGG AGCAGGCAGA GCCAGATGGC 
ACAGAAGTGG CTGACAAGGC TGCCTACCTG ATGGGCCTGA ATTCAGCTGA CATGCTCAAG 
GCACTATGCT ACCCTCGAGT CAAAGTTGGG AATGAATATG TGACCAAGGG TCAGACTGTA 
CAACAGGTGC ACAATGCTGT AGGTGCTCTG GCAAAGGCTG TCTATGAGAG GATGTTCTTG 
TGGATGGTTG TTCGCATCAA CCAGCAGCTG GATACCAAGC AGCCCAGGCA GTACTTCATT 
GGTGTCCTGG ACATCGCTGG CTTTGAGATC TTTGATTTCA ACAGCTTCGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT TGTGCTGGAG 
CAAGAGGAGT ACAAAAAGGA AGGCATTGAA TGGACATTCA TTGACTTTGG GATGGACCTG 
GCTGCCTGCA TTGAGCTCAT TGAGAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCAAC CGACACCTCT TTCAAGAACA AGCTCTATGA CCAGCATCTG 
GGCAAGTCCA GCAACTTCCA GAAGCCCAAG CCTACCAAAG GAAAGGTTGA GGCCCACTTC 
TCCCTAATAC ACTATGCTGG CACAGTGGAC TACAATATCA CTGGCTGGCT TGAGAAGAAC 
AAGGACCCCC TGAATGAAAC TGTCATTGGG CTGTACCAGA AATCTTCACT GAAAACTTTG 
GCCTTACTGT TTGCCAACTA TGGTGGAGCA GAAGCAGAGG CTAGTGCTGG TGCTGGCAAG 
AAAGGTGGCA AGAAGAAAGG TTCTTCTTTC CAGACTGTCT CAGCTCTTTT CCGGGAGAAC 
TTAAACAAGC TGATGACCAA TCTGAGGAGC ACCCACCCCC ATTTTGTACG CTGTATCATC 
CCAAATGAAA CAAAAACACC TGGTAAGAGA CTCACCCACA GACAACCTTC AGTATCTCTT 
CTCTGCTGCG CTACAGAATA CAGCACTCAT TTATACTCTG AATTGCTAGG TGCCATGGAG 
CATGAGCTGG TTCTGCACCA ACTGAGGTGT AATGGTGTGC TGGAAGGGAT CAGAATTTGC 
AGGAAAGGAT TTCCCAACAG AGTCCTGTAT GCAGATTTTA AACAGAGATA CAAAGTACTA 
AATGCAAGCG CTATTCCAGA GGGACAGTTC ATTGACAGCA AGAAGGCTTG TGAGAAACTG 
CTTGGATCAA TTGATATAGA CCACACTCAG TATAAATTTG GTCACACCAA GGTGTTCTTC 
AAAGCTGGGC TGGTTGGCCT TTTGGAGGAG ATGAGGGATG AGAAGCTGGC ACAGCTCATC 
ACTCGCACAC AGGCCAGGTG CAGAGGCTTC CTGATGAGAG TGGAATACCA GAAAATGGTG 
GAGAGGAGAG AGTCTGTCTT CTGCATCCAG TACAACATTC GTGCATTCAT GAATGTCAAG 
CACTGGCCCT GGATGAAGCT GTTCTTTAAG ATCAAGCCGC TGCTGAAGAG CGCAGAGTCT 
GAGAAGGAGA TGGCCAACAT GAAACAAGAG TTTGAGAAGA CCAAGGAAGA GCTTGCAAAG 
TCTGAGGCAA AGAGGAAGGA GCTGGAGGAG AAAATGGTGA AGCTGGTGCA GGAGAAAAAT 
GATCTGCAGC TCCAAGTGCA GGCTGAAGCT GATGCATTGG CTGATGCTGA GGAAAGATGT 
GACCAGCTCA TCAAAACCAA AATCCAGCTG GAAGCCAAAG TAAAGGAGGT GACAGAACGG 
GCTGAGGATG AGGAGGAAAT TAATGCTGAA CTGACAGCCA AGAAGAGAAA ACTGGAGGAT 
GAATGTTCAG AACTGAAGAA AGACATAGAT GACCTGGAGT TAACATTGGC CAAAGTTGAG 
AAGGAAAAGC ATGCCACTGA AAACAAGGTG AAAAACCTCA CAGAGGAGAT GGCAGCCCTG 
GATGAGACCA TTGTGAAGCT GACAAAAGAG AAGAAAGCCC TCCAAGAGGC CCATCAGCAG 
ACACTGGATG ACCTGCAGGC TGAAGAGGAC AAAGTCAATA CGCTGACCAA AGCTAAAACC 
AAGCTGGAAC AGCAAGTGGA TGATCTGGAA GGGTCCCTGG AGCAAGAGAA GAAGCTGCGC 
ATGGACCTCG AGAGAGCCAA GAGGAAACTT GAAGGAGACC TGAAGTTGGC CCATGATAGC 
ATAATGGACT TGGAAAATGA TAAACAGCAG CTGGATGAGA AACTGAAGAA GAAAGACTTT 
GAAATCAGCC AGATCCAGAG CAAAATTGAG GATGAACAAC TTCTGGGCAT GCAGTTACAG 
AAGAAGATCA AGGAGCTGCA GGCCCGTATT GAAGAACTGG AGGAGGAAAT TGAGGCAGAG 
CGAACCTCTC GGGCAAAAGC AGAGAAGCAC CGAGCTGACC TCTCCAGGGA GCTGGAGGAG 
ATCAGTGGGC GCCTGGAGGA AGCAGGAGGG GCTACAGCAG CTCAGATCGA GATGAACAAG 
AAGCGTGAGG CAGAATTTCA GAAGATGCGC CGTGACCTCG AAGAGGCCAC GCTGCAGCAC 
GAAGCCACGG CTGCTGCCCT GCGGAAGAAG CATGCGGACA GCACAGCGGA GCTTGGGGAG 
CAGATTGACA ACCTGCAACG AGTCAAGCAG AAGCTGGAGA AGGAGAAGAG TGAGCTGAAG 
ATGGAGATTG ATGACTTGGC CAGCAACATG GAGTCTGTCT CCAAAGCTAA GGCAAACCTG 
GAGAAGATGT GTCGCACTTT GGAAGACCAA CTAAGTGAAT TCAAAACGAA AGATGAGCAG 
AACCAGCGCA TGATCAGTGA TCTCAGTGCT CAAAGAGCTC GCCTGCAAAC AGAATCTGGT 
GAATACGCAC GCCAGGCGGA CGAAAAAGAT GGTTTAATTT CTCAGCTGTC CAGAGGGAAA 
CAGGCCTTCA CGCAACAGAT TGAAGAACTA AAGCGGCAAC TAGAAGAAGA GATAAAGGCC 
AAGAATGCCC TGGCCCACAG CTTGCAGTCA GCCCGCCATG ACTGTGATTT GCTCCGGGAA 
CAATATGAGG AGGAGCTTGA AGCCAAGGGT GAGCTGCAGC GTGCCCTGTC CAAGGCCAAC 
AGCGAAGTGG CCCAGTGGAG AACCAAATAT GAGACGGATG CTATTCAGCG CACAGAGGAG 
CTGGAGGAGG CCAAGAAGAA GCTGGCACAG CGCCTGCAGG ATGCCGAGGA ACATGTTGAA 
GCTGTGAATG CCAAATGCGC TTCCCTGGAA AAGACAAAGC AGAGGCTGCA GAATGAGGTG 
GAGGACCTGA TGATTGACGT GGAGCGAGCA AATGCTGCCT GTGCAGCTCT GGACAAGAAG 
CAGAAGAACT TTGACAAGAT CCTGGCAGAG TGGAAGCAGA AGTACGAGGA AACGCAGGCT 
GAGCTGGAGG CCTCCCAGAA GGAGTCTCGC TCCCTCAGCA CAGAGCTGTT TAAGATGAAG 
AATGCCTATG AGGAGTCCCT GGACCACCTG GAAACGCTGA AGCGTGAGAA CAAGAACTTG 
CAGCAGGAGA TTTCTGACCT CACAGAGCAG ATTGCAGAGG GAGGAAAGGC GATTCATGAG 
TTGGAGAAAG TCAAGAAGCA GATTGAGCAG GAGAAGTCTG AAATCCAGGC AGCACTGGAG 
GAAGCTGAGG CCTCCCTGGA ACATGAAGAG GGGAAGATCC TGCGCCTCCA ACTGGAGCTC 
AACCAGGTCA AGTCTGAGAT CGACAGGAAG ATAGCAGAGA AAGATGAGGA AATTGACCAG 
CTGAAGAGAA ATCACCTCAG AATTGTGGAG TCCTTGCAGA GCAGCCTGGA TGCTGAGATC 
AGGAGCAGGA ATGAGGCCCT GAGGCTGAAG AAGAAGATGG AGGGAGACCT GAATGAAATG 
GAGATCCAGC TGAGCCACGC TAACCGTGTT GCTGCAGAGG CACAAAAGAA CCTGAGAAAC 
ACACAGGCAG TGTTGAAGGA TACCCAGATA CATTTGGACG ACGCTCTCAG GACACAGGAG 
GACCTGAAGG AGCAGGTGGC CATGGTGGAG CGCAGAGCAA ACCTGTTGCA GGCTGAGATT 
GAGGAGCTAC GGGCAGCCCT GGAGCAGACA GAGCGCTCGA GAAAAGTGGC TGAGCAGGAA 
CTGATGGATG CAAGTGAGCG TGTGCAGCTC CTCCATACCC AGAACACCAG CTTGATCAAC 
ACCAAGAAGA AACTAGAAAC GGACATTGCC CAAATTCAGA GTGAAATGGA GGATACCATC 
CAGGAAGCCC GCAATGCTGA AGAGAAGGCC AAGAAGGCCA TCACCGATGC AGCCATGATG 
GCAGAAGAGC TGAAGAAGGA GCAGGACACC AGTGCCCACC TGGAGAGGAT GAAGAAGAAC 
CTGGACCAGA CGGTGAAGGA CCTGCAGCAC CGCCTGGATG AGGCAGAGCA GCTGGCCCTG 
AAGGGAGGCA AGAAGCAAAT TCAGAAGCTG GAGGCCAGGG TGCGGGAGCT GGAAGGGGAG 
GTTGATGCTG AGCAGAAGCG CAGCGCTGAA GCCGTGAAGG GTGTGCGCAA GTACGAGAGG 
AGGGTGAAGG AGCTGACCTA CCAGTCTGAG GAAGATCGCA AGAACATTCT TAGACTCCAG 
GATCTGGTGG ACAAACTACA GATGAAGGTG AAATCCTACA AGAGACAATC TGAGGAGGCT 
GAGGAGCTGT CCAATGTCAA CCTTTCCAAG TTCCGCAAGA TCCAGCACGA GCTGGAGGAG 
GCTGAGGAGC GGGCTGACAT TGCAGAGTCA CAGGTCAACA AGCTCCGAGC AAAGAGCCGG 
GAATTCCATA GGAGGATTGA AGAGGAAGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024997495.1
CDS	6076..11988
Translation	MASSDAEMAVFGEAAPYLRKSEKERIEAQNKPFDAKSSVFVAHPKESFVKGTIQSRETGKVTVKTEGGETLTVKEDQVFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVLAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAASGEKKKEEQSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLKAERSYHIFYQVTSNKKPELIEMLLITTNPYDYPFVSQGEITVPSIDDKEELMATDSAIDILGFTADEKTAIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLMGLNSADMLKALCYPRVKVGNEYVTKGQTVQQVHNAVGALAKAVYERMFLWMVVRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSSNFQKPKPTKGKVEAHFSLIHYAGTVDYNITGWLEKNKDPLNETVIGLYQKSSLKTLALLFANYGGAEAEASAGAGKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGKRLTHRQPSVSLLCCATEYSTHLYSELLGAMEHELVLHQLRCNGVLEGIRICRKGFPNRVLYADFKQRYKVLNASAIPEGQFIDSKKACEKLLGSIDIDHTQYKFGHTKVFFKAGLVGLLEEMRDEKLAQLITRTQARCRGFLMRVEYQKMVERRESVFCIQYNIRAFMNVKHWPWMKLFFKIKPLLKSAESEKEMANMKQEFEKTKEELAKSEAKRKELEEKMVKLVQEKNDLQLQVQAEADALADAEERCDQLIKTKIQLEAKVKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAALDETIVKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAHDSIMDLENDKQQLDEKLKKKDFEISQIQSKIEDEQLLGMQLQKKIKELQARIEELEEEIEAERTSRAKAEKHRADLSRELEEISGRLEEAGGATAAQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSTAELGEQIDNLQRVKQKLEKEKSELKMEIDDLASNMESVSKAKANLEKMCRTLEDQLSEFKTKDEQNQRMISDLSAQRARLQTESGEYARQADEKDGLISQLSRGKQAFTQQIEELKRQLEEEIKAKNALAHSLQSARHDCDLLREQYEEELEAKGELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERANAACAALDKKQKNFDKILAEWKQKYEETQAELEASQKESRSLSTELFKMKNAYEESLDHLETLKRENKNLQQEISDLTEQIAEGGKAIHELEKVKKQIEQEKSEIQAALEEAEASLEHEEGKILRLQLELNQVKSEIDRKIAEKDEEIDQLKRNHLRIVESLQSSLDAEIRSRNEALRLKKKMEGDLNEMEIQLSHANRVAAEAQKNLRNTQAVLKDTQIHLDDALRTQEDLKEQVAMVERRANLLQAEIEELRAALEQTERSRKVAEQELMDASERVQLLHTQNTSLINTKKKLETDIAQIQSEMEDTIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLDQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVDAEQKRSAEAVKGVRKYERRVKELTYQSEEDRKNILRLQDLVDKLQMKVKSYKRQSEEAEELSNVNLSKFRKIQHELEEAEERADIAESQVNKLRAKSREFHRRIEEEE

Target ORF information:

RefSeq Version	XM_025141727.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus myosin, heavy chain 1G, skeletal muscle (MYH1G), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025141727.1

ORF Insert Sequence:

ATGGCTTCTT CAGATGCTGA GATGGCTGTC TTTGGAGAGG CAGCTCCCTA CCTCCGAAAG 
TCAGAGAAGG AAAGAATCGA GGCCCAGAAC AAGCCTTTTG ATGCCAAGTC ATCAGTCTTT 
GTGGCCCATC CAAAGGAATC CTTTGTGAAA GGGACAATCC AGAGCAGGGA AACAGGGAAG 
GTCACTGTCA AGACTGAAGG TGGAGAGACT CTGACTGTGA AGGAAGATCA GGTCTTCTCC 
ATGAACCCTC CCAAGTACGA TAAAATTGAG GACATGGCCA TGATGACCCA CCTCCATGAA 
CCCGCTGTGC TGTACAACCT CAAAGAGCGT TATGCAGCCT GGATGATCTA CACCTACTCG 
GGCCTCTTCT GTGTCACTGT CAACCCCTAC AAGTGGCTGC CGGTGTACAA CCCGGAGGTG 
GTGTTGGCCT ACCGAGGCAA AAAGCGCCAG GAGGCCCCTC CACACATCTT CTCCATCTCT 
GACAACGCCT ATCAGTTCAT GCTGACTGAC CGTGAGAATC AGTCGATCCT GATCACCGGA 
GAATCCGGTG CAGGGAAGAC TGTGAACACG AAACGTGTCA TCCAGTACTT TGCAACAATT 
GCAGCTAGTG GGGAGAAGAA GAAGGAGGAG CAGTCTGGCA AAATGCAGGG AACACTTGAG 
GATCAAATCA TCAGTGCCAA CCCCCTGCTG GAGGCCTTTG GAAATGCCAA GACTGTGAGG 
AATGACAACT CCTCACGCTT TGGTAAATTC ATCAGAATCC ATTTTGGGGC CACAGGCAAA 
CTGGCTTCTG CTGACATTGA AACATATCTG CTGGAAAAGT CCAGGGTTAC TTTCCAGCTC 
AAGGCAGAAA GAAGCTACCA CATCTTTTAT CAGGTCACCT CCAACAAGAA GCCAGAGCTA 
ATAGAAATGC TCCTTATCAC CACCAACCCA TATGACTACC CTTTTGTGAG TCAAGGTGAG 
ATCACTGTCC CCAGCATTGA TGACAAAGAG GAGCTTATGG CTACAGATAG TGCCATTGAC 
ATCCTGGGCT TCACTGCTGA TGAAAAGACT GCCATCTACA AGCTGACAGG GGCTGTCATG 
CACTACGGGA ATTTGAAGTT CAAGCAGAAA CAGCGAGAGG AGCAGGCAGA GCCAGATGGC 
ACAGAAGTGG CTGACAAGGC TGCCTACCTG ATGGGCCTGA ATTCAGCTGA CATGCTCAAG 
GCACTATGCT ACCCTCGAGT CAAAGTTGGG AATGAATATG TGACCAAGGG TCAGACTGTA 
CAACAGGTGC ACAATGCTGT AGGTGCTCTG GCAAAGGCTG TCTATGAGAG GATGTTCTTG 
TGGATGGTTG TTCGCATCAA CCAGCAGCTG GATACCAAGC AGCCCAGGCA GTACTTCATT 
GGTGTCCTGG ACATCGCTGG CTTTGAGATC TTTGATTTCA ACAGCTTCGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT TGTGCTGGAG 
CAAGAGGAGT ACAAAAAGGA AGGCATTGAA TGGACATTCA TTGACTTTGG GATGGACCTG 
GCTGCCTGCA TTGAGCTCAT TGAGAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCAAC CGACACCTCT TTCAAGAACA AGCTCTATGA CCAGCATCTG 
GGCAAGTCCA GCAACTTCCA GAAGCCCAAG CCTACCAAAG GAAAGGTTGA GGCCCACTTC 
TCCCTAATAC ACTATGCTGG CACAGTGGAC TACAATATCA CTGGCTGGCT TGAGAAGAAC 
AAGGACCCCC TGAATGAAAC TGTCATTGGG CTGTACCAGA AATCTTCACT GAAAACTTTG 
GCCTTACTGT TTGCCAACTA TGGTGGAGCA GAAGCAGAGG CTAGTGCTGG TGCTGGCAAG 
AAAGGTGGCA AGAAGAAAGG TTCTTCTTTC CAGACTGTCT CAGCTCTTTT CCGGGAGAAC 
TTAAACAAGC TGATGACCAA TCTGAGGAGC ACCCACCCCC ATTTTGTACG CTGTATCATC 
CCAAATGAAA CAAAAACACC TGGTAAGAGA CTCACCCACA GACAACCTTC AGTATCTCTT 
CTCTGCTGCG CTACAGAATA CAGCACTCAT TTATACTCTG AATTGCTAGG TGCCATGGAG 
CATGAGCTGG TTCTGCACCA ACTGAGGTGT AATGGTGTGC TGGAAGGGAT CAGAATTTGC 
AGGAAAGGAT TTCCCAACAG AGTCCTGTAT GCAGATTTTA AACAGAGATA CAAAGTACTA 
AATGCAAGCG CTATTCCAGA GGGACAGTTC ATTGACAGCA AGAAGGCTTG TGAGAAACTG 
CTTGGATCAA TTGATATAGA CCACACTCAG TATAAATTTG GTCACACCAA GGTGTTCTTC 
AAAGCTGGGC TGGTTGGCCT TTTGGAGGAG ATGAGGGATG AGAAGCTGGC ACAGCTCATC 
ACTCGCACAC AGGCCAGGTG CAGAGGCTTC CTGATGAGAG TGGAATACCA GAAAATGGTG 
GAGAGGAGAG AGTCTGTCTT CTGCATCCAG TACAACATTC GTGCATTCAT GAATGTCAAG 
CACTGGCCCT GGATGAAGCT GTTCTTTAAG ATCAAGCCGC TGCTGAAGAG CGCAGAGTCT 
GAGAAGGAGA TGGCCAACAT GAAACAAGAG TTTGAGAAGA CCAAGGAAGA GCTTGCAAAG 
TCTGAGGCAA AGAGGAAGGA GCTGGAGGAG AAAATGGTGA AGCTGGTGCA GGAGAAAAAT 
GATCTGCAGC TCCAAGTGCA GGCTGAAGCT GATGCATTGG CTGATGCTGA GGAAAGATGT 
GACCAGCTCA TCAAAACCAA AATCCAGCTG GAAGCCAAAG TAAAGGAGGT GACAGAACGG 
GCTGAGGATG AGGAGGAAAT TAATGCTGAA CTGACAGCCA AGAAGAGAAA ACTGGAGGAT 
GAATGTTCAG AACTGAAGAA AGACATAGAT GACCTGGAGT TAACATTGGC CAAAGTTGAG 
AAGGAAAAGC ATGCCACTGA AAACAAGGTG AAAAACCTCA CAGAGGAGAT GGCAGCCCTG 
GATGAGACCA TTGTGAAGCT GACAAAAGAG AAGAAAGCCC TCCAAGAGGC CCATCAGCAG 
ACACTGGATG ACCTGCAGGC TGAAGAGGAC AAAGTCAATA CGCTGACCAA AGCTAAAACC 
AAGCTGGAAC AGCAAGTGGA TGATCTGGAA GGGTCCCTGG AGCAAGAGAA GAAGCTGCGC 
ATGGACCTCG AGAGAGCCAA GAGGAAACTT GAAGGAGACC TGAAGTTGGC CCATGATAGC 
ATAATGGACT TGGAAAATGA TAAACAGCAG CTGGATGAGA AACTGAAGAA GAAAGACTTT 
GAAATCAGCC AGATCCAGAG CAAAATTGAG GATGAACAAC TTCTGGGCAT GCAGTTACAG 
AAGAAGATCA AGGAGCTGCA GGCCCGTATT GAAGAACTGG AGGAGGAAAT TGAGGCAGAG 
CGAACCTCTC GGGCAAAAGC AGAGAAGCAC CGAGCTGACC TCTCCAGGGA GCTGGAGGAG 
ATCAGTGGGC GCCTGGAGGA AGCAGGAGGG GCTACAGCAG CTCAGATCGA GATGAACAAG 
AAGCGTGAGG CAGAATTTCA GAAGATGCGC CGTGACCTCG AAGAGGCCAC GCTGCAGCAC 
GAAGCCACGG CTGCTGCCCT GCGGAAGAAG CATGCGGACA GCACAGCGGA GCTTGGGGAG 
CAGATTGACA ACCTGCAACG AGTCAAGCAG AAGCTGGAGA AGGAGAAGAG TGAGCTGAAG 
ATGGAGATTG ATGACTTGGC CAGCAACATG GAGTCTGTCT CCAAAGCTAA GGCAAACCTG 
GAGAAGATGT GTCGCACTTT GGAAGACCAA CTAAGTGAAT TCAAAACGAA AGATGAGCAG 
AACCAGCGCA TGATCAGTGA TCTCAGTGCT CAAAGAGCTC GCCTGCAAAC AGAATCTGGT 
GAATACGCAC GCCAGGCGGA CGAAAAAGAT GGTTTAATTT CTCAGCTGTC CAGAGGGAAA 
CAGGCCTTCA CGCAACAGAT TGAAGAACTA AAGCGGCAAC TAGAAGAAGA GATAAAGGCC 
AAGAATGCCC TGGCCCACAG CTTGCAGTCA GCCCGCCATG ACTGTGATTT GCTCCGGGAA 
CAATATGAGG AGGAGCTTGA AGCCAAGGGT GAGCTGCAGC GTGCCCTGTC CAAGGCCAAC 
AGCGAAGTGG CCCAGTGGAG AACCAAATAT GAGACGGATG CTATTCAGCG CACAGAGGAG 
CTGGAGGAGG CCAAGAAGAA GCTGGCACAG CGCCTGCAGG ATGCCGAGGA ACATGTTGAA 
GCTGTGAATG CCAAATGCGC TTCCCTGGAA AAGACAAAGC AGAGGCTGCA GAATGAGGTG 
GAGGACCTGA TGATTGACGT GGAGCGAGCA AATGCTGCCT GTGCAGCTCT GGACAAGAAG 
CAGAAGAACT TTGACAAGAT CCTGGCAGAG TGGAAGCAGA AGTACGAGGA AACGCAGGCT 
GAGCTGGAGG CCTCCCAGAA GGAGTCTCGC TCCCTCAGCA CAGAGCTGTT TAAGATGAAG 
AATGCCTATG AGGAGTCCCT GGACCACCTG GAAACGCTGA AGCGTGAGAA CAAGAACTTG 
CAGCAGGAGA TTTCTGACCT CACAGAGCAG ATTGCAGAGG GAGGAAAGGC GATTCATGAG 
TTGGAGAAAG TCAAGAAGCA GATTGAGCAG GAGAAGTCTG AAATCCAGGC AGCACTGGAG 
GAAGCTGAGG CCTCCCTGGA ACATGAAGAG GGGAAGATCC TGCGCCTCCA ACTGGAGCTC 
AACCAGGTCA AGTCTGAGAT CGACAGGAAG ATAGCAGAGA AAGATGAGGA AATTGACCAG 
CTGAAGAGAA ATCACCTCAG AATTGTGGAG TCCTTGCAGA GCAGCCTGGA TGCTGAGATC 
AGGAGCAGGA ATGAGGCCCT GAGGCTGAAG AAGAAGATGG AGGGAGACCT GAATGAAATG 
GAGATCCAGC TGAGCCACGC TAACCGTGTT GCTGCAGAGG CACAAAAGAA CCTGAGAAAC 
ACACAGGCAG TGTTGAAGGA TACCCAGATA CATTTGGACG ACGCTCTCAG GACACAGGAG 
GACCTGAAGG AGCAGGTGGC CATGGTGGAG CGCAGAGCAA ACCTGTTGCA GGCTGAGATT 
GAGGAGCTAC GGGCAGCCCT GGAGCAGACA GAGCGCTCGA GAAAAGTGGC TGAGCAGGAA 
CTGATGGATG CAAGTGAGCG TGTGCAGCTC CTCCATACCC AGAACACCAG CTTGATCAAC 
ACCAAGAAGA AACTAGAAAC GGACATTGCC CAAATTCAGA GTGAAATGGA GGATACCATC 
CAGGAAGCCC GCAATGCTGA AGAGAAGGCC AAGAAGGCCA TCACCGATGC AGCCATGATG 
GCAGAAGAGC TGAAGAAGGA GCAGGACACC AGTGCCCACC TGGAGAGGAT GAAGAAGAAC 
CTGGACCAGA CGGTGAAGGA CCTGCAGCAC CGCCTGGATG AGGCAGAGCA GCTGGCCCTG 
AAGGGAGGCA AGAAGCAAAT TCAGAAGCTG GAGGCCAGGG TGCGGGAGCT GGAAGGGGAG 
GTTGATGCTG AGCAGAAGCG CAGCGCTGAA GCCGTGAAGG GTGTGCGCAA GTACGAGAGG 
AGGGTGAAGG AGCTGACCTA CCAGTCTGAG GAAGATCGCA AGAACATTCT TAGACTCCAG 
GATCTGGTGG ACAAACTACA GATGAAGGTG AAATCCTACA AGAGACAATC TGAGGAGGCT 
GAGGAGCTGT CCAATGTCAA CCTTTCCAAG TTCCGCAAGA TCCAGCACGA GCTGGAGGAG 
GCTGAGGAGC GGGCTGACAT TGCAGAGTCA CAGGTCAACA AGCTCCGAGC AAAGAGCCGG 
GAATTCCATA GGAGGATTGA AGAGGAAGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A canonical sequence organization at the 5'-end of the myosin heavy chain genes.
The Journal of biological chemistry261(14)6613-8(1986 May)
Kropp K,Gulick J,Robbins J

The chicken myosin heavy chain family.
The Journal of biological chemistry261(14)6606-12(1986 May)
Robbins J,Horan T,Gulick J,Kropp K

The proteolytic substructure of light meromyosin. Localization of a region responsible for the low ionic strength insolubility of myosin.
The Journal of biological chemistry258(21)13213-20(1983 Nov)
Nyitray L,Mocz G,Szilagyi L,Balint M,Lu RC,Wong A,Gergely J

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MYH1G cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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