CEBPA cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	CEBPA
Entrez Gene ID	427549
Full Name	CCAAT/enhancer binding protein alpha
Synonyms	cC/EBP
General protein information	Preferred Names CCAAT/enhancer binding protein alpha Names CCAAT/enhancer-binding protein alpha CCAAT/enhancer binding protein (C/EBP), alpha
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001031459.1	NP_001026630.1	CCAAT/enhancer-binding protein alpha

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CEBPA	NP_789741.2
Homo sapiens (human)	CEBPA	NP_001274353.1
Pan troglodytes (chimpanzee)	CEBPA	XP_001154519.2
Mus musculus (house mouse)	Cebpa	NP_031704.2
Danio rerio (zebrafish)	cebpa	NP_571960.1
Xenopus tropicalis (tropical clawed frog)	cebpa	NP_001011044.1
	LOC717153	XP_001108401.1
Gallus gallus (chicken)	CEBPA	NP_001026630.1
Rattus norvegicus (Norway rat)	Cebpa	NP_036656.1

Gene Ontology
Bibliography

Related articles in PubMed

CpG site DNA methylation of the CCAAT/enhancer-binding protein, alpha promoter in chicken lines divergently selected for fatness.
Gao Y, Sun Y, Duan K, Shi H, Wang S, Li H, Wang N
Animal genetics46(4)410-7(2015 Aug)

Folate supplementation modifies CCAAT/enhancer-binding protein ? methylation to mediate differentiation of preadipocytes in chickens.
Yu X, Liu R, Zhao G, Zheng M, Chen J, Wen J
Poultry science93(10)2596-603(2014 Oct)

Transcriptional regulation of chicken cytochrome P450 2D49 basal expression by CCAAT/enhancer-binding protein ? and hepatocyte nuclear factor 4?.
Yang Q, Tang S, Dong L, Chen Q, Liu X, Jiang J, Deng Y
The FEBS journal281(5)1379-92(2014 Mar)

Expression profiles of key transcription factors involved in lipid metabolism in Beijing-You chickens.
Fu RQ, Liu RR, Zhao GP, Zheng MQ, Chen JL, Wen J
Gene537(1)120-5(2014 Mar)

Transdifferentiation of fibroblasts into adipocyte-like cells by chicken adipogenic transcription factors.
Liu S, Wang Y, Wang L, Wang N, Li Y, Li H
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology156(4)502-8(2010 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CEBPA promoter is methylated in adipose tissue and may regulate chicken early adipose development
Title: CpG site DNA methylation of the CCAAT/enhancer-binding protein, alpha promoter in chicken lines divergently selected for fatness.

Folate caused a dose-dependent decrease in transcript abundance of peroxisome proliferator-activated receptor gamma (PPARgamma), CCAAT/enhancer-binding protein alpha (C/EBPalpha) gene expression, and the downstream enzyme fatty acid synthase.
Title: Folate supplementation modifies CCAAT/enhancer-binding protein ? methylation to mediate differentiation of preadipocytes in chickens.

The results of overexpressing C/EBPalpha and HNF4alpha in cultured cells confirmed that both C/EBPalpha and HNF4alpha contribute significantly to sustaining a high level of CYP2D49 transcription.
Title: Transcriptional regulation of chicken cytochrome P450 2D49 basal expression by CCAAT/enhancer-binding protein ? and hepatocyte nuclear factor 4?.

CEBPA involved in lipid metabolism in Beijing-You chickens.
Title: Expression profiles of key transcription factors involved in lipid metabolism in Beijing-You chickens.

The result showed C-EBP alpha was sufficient to trigger the adipogenic program in chicken embryo fibroblasts, as demonstrated by accumulation of cytoplasmic lipid droplets and expression of the adipocyte marker gene.
Title: Transdifferentiation of fibroblasts into adipocyte-like cells by chicken adipogenic transcription factors.

The following CEBPA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CEBPA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa00408	NM_001031459.1 Latest version!	Gallus gallus CCAAT/enhancer binding protein alpha (CEBPA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CEBPA ( NM_001031459.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001026630.1 Gallus gallus CCAAT/enhancer binding protein alpha (CEBPA), mRNA.

Price & Availability↑

CloneID	OGa00408
Clone ID Related Accession (Same CDS sequence)	NM_001031459.1
Accession Version	NM_001031459.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-11
Organism	Gallus gallus(chicken)
Product	CCAAT/enhancer-binding protein alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X66844.1. On Aug 8, 2005 this sequence version replaced XM_425121.1.

ATGGAGCAAG CCAACTTCTA CGAGGTCGAT TCCCGGCCCC CGATGAGCAG CGGCCAGCAC 
CACCAGCTCC AGACTCCCCT GCCCGGCAGC GCCTACGGCT ACAGAGAGGC TCCCTCGGCG 
GCGGCACCTG CTGCGGGCGG CGCGGAGCTC GGCGACATCT GCGAGAACGA GCACTCCATC 
GACATCAGCG CCTACATCGA CCCCGCCGCC TTCAACGACG AGTTCCTGGC CGACCTCTTC 
CAGCACAGCA AGCAGCAGGA GAAGCACAAG GCCGTCCTGG CCGGGGATTT CGATTTCCAC 
GGCATGCACG GGGCCGGCGC GGCCGCCTCG GCGCCGGGGC ACCACCCGCA GCACCAGCAG 
CAGCCGCTGT TCGGCTGCGC GGCCGGCTAC ATGGACGGCA AGCTCGACCC GCTGTACGAG 
CGCATCGCCG CGCCGGGGTT GCGGCCGCTG GTCATCAAGC AGGAGCCCCG CGAGGAGGAG 
GAGGTGAAGG CGGCGGCCCT GGCGGCCCTC TACCCGCACC CGCAGCAGCA CCCGTCCCAC 
CTGCAGTACC AGATCGCCCA CTGCGCGCAG ACCACCGTGC ACCTGCAGCC CGGGCACCCC 
ACGCCGCCCC CCACGCCCGT GCCCAGCCCG CACCACCCGC ACCACCCGCA CCCCCCGGGC 
GCGCTGCCCG CCGCTCCCGG CGCCCTCAAG ATGATGCCCG CCGACCACCG CGGCAAATCC 
AAAAAGACAG TGGACAAGAA CAGCAACGAG TACCGCGTGC GCCGGGAGCG CAACAACATC 
GCGGTGCGCA AGAGCCGGGA CAAGGCCAAG CAGCGCAACG TGGAGACGCA GCAGAAGGTG 
CTGGAGCTCA CCACCGACAA CGAGCGGCTG CGGAAGCGGG TGGAGCAGCT CAGCCGGGAG 
CTGGAGACTC TGCGGGGCAT CTTCAGGCAG CTGCCCGAAA GCTCGCTGGT GAAGGCCATG 
GGCAGCTGCG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001026630.1
CDS	1..975
Translation	MEQANFYEVDSRPPMSSGQHHQLQTPLPGSAYGYREAPSAAAPAAGGAELGDICENEHSIDISAYIDPAAFNDEFLADLFQHSKQQEKHKAVLAGDFDFHGMHGAGAAASAPGHHPQHQQQPLFGCAAGYMDGKLDPLYERIAAPGLRPLVIKQEPREEEEVKAAALAALYPHPQQHPSHLQYQIAHCAQTTVHLQPGHPTPPPTPVPSPHHPHHPHPPGALPAAPGALKMMPADHRGKSKKTVDKNSNEYRVRRERNNIAVRKSRDKAKQRNVETQQKVLELTTDNERLRKRVEQLSRELETLRGIFRQLPESSLVKAMGSCA

Target ORF information:

RefSeq Version	NM_001031459.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus CCAAT/enhancer binding protein alpha (CEBPA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001031459.1

ORF Insert Sequence:

ATGGAGCAAG CCAACTTCTA CGAGGTCGAT TCCCGGCCCC CGATGAGCAG CGGCCAGCAC 
CACCAGCTCC AGACTCCCCT GCCCGGCAGC GCCTACGGCT ACAGAGAGGC TCCCTCGGCG 
GCGGCACCTG CTGCGGGCGG CGCGGAGCTC GGCGACATCT GCGAGAACGA GCACTCCATC 
GACATCAGCG CCTACATCGA CCCCGCCGCC TTCAACGACG AGTTCCTGGC CGACCTCTTC 
CAGCACAGCA AGCAGCAGGA GAAGCACAAG GCCGTCCTGG CCGGGGATTT CGATTTCCAC 
GGCATGCACG GGGCCGGCGC GGCCGCCTCG GCGCCGGGGC ACCACCCGCA GCACCAGCAG 
CAGCCGCTGT TCGGCTGCGC GGCCGGCTAC ATGGACGGCA AGCTCGACCC GCTGTACGAG 
CGCATCGCCG CGCCGGGGTT GCGGCCGCTG GTCATCAAGC AGGAGCCCCG CGAGGAGGAG 
GAGGTGAAGG CGGCGGCCCT GGCGGCCCTC TACCCGCACC CGCAGCAGCA CCCGTCCCAC 
CTGCAGTACC AGATCGCCCA CTGCGCGCAG ACCACCGTGC ACCTGCAGCC CGGGCACCCC 
ACGCCGCCCC CCACGCCCGT GCCCAGCCCG CACCACCCGC ACCACCCGCA CCCCCCGGGC 
GCGCTGCCCG CCGCTCCCGG CGCCCTCAAG ATGATGCCCG CCGACCACCG CGGCAAATCC 
AAAAAGACAG TGGACAAGAA CAGCAACGAG TACCGCGTGC GCCGGGAGCG CAACAACATC 
GCGGTGCGCA AGAGCCGGGA CAAGGCCAAG CAGCGCAACG TGGAGACGCA GCAGAAGGTG 
CTGGAGCTCA CCACCGACAA CGAGCGGCTG CGGAAGCGGG TGGAGCAGCT CAGCCGGGAG 
CTGGAGACTC TGCGGGGCAT CTTCAGGCAG CTGCCCGAAA GCTCGCTGGT GAAGGCCATG 
GGCAGCTGCG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CpG site DNA methylation of the CCAAT/enhancer-binding protein, alpha promoter in chicken lines divergently selected for fatness.
Animal genetics46(4)410-7(2015 Aug)
Gao Y,Sun Y,Duan K,Shi H,Wang S,Li H,Wang N

Folate supplementation modifies CCAAT/enhancer-binding protein ? methylation to mediate differentiation of preadipocytes in chickens.
Poultry science93(10)2596-603(2014 Oct)
Yu X,Liu R,Zhao G,Zheng M,Chen J,Wen J

Transcriptional regulation of chicken cytochrome P450 2D49 basal expression by CCAAT/enhancer-binding protein ? and hepatocyte nuclear factor 4?.
The FEBS journal281(5)1379-92(2014 Mar)
Yang Q,Tang S,Dong L,Chen Q,Liu X,Jiang J,Deng Y

Expression profiles of key transcription factors involved in lipid metabolism in Beijing-You chickens.
Gene537(1)120-5(2014 Mar)
Fu RQ,Liu RR,Zhao GP,Zheng MQ,Chen JL,Wen J

Transdifferentiation of fibroblasts into adipocyte-like cells by chicken adipogenic transcription factors.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology156(4)502-8(2010 Aug)
Liu S,Wang Y,Wang L,Wang N,Li Y,Li H

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CEBPA cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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