ESPL1 cDNA ORF clone, Gallus gallus(chicken)

The following ESPL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ESPL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa50164 XM_015273047.2
Latest version!
Gallus gallus extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OGa50165 XM_025145487.1
Latest version!
Gallus gallus extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OGa50165 XM_015273048.1
Latest version!
Gallus gallus extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
View Old Accession Versions >>
OGa50164 XM_015273047.1 Gallus gallus extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa50164
    Clone ID Related Accession (Same CDS sequence) XM_015273047.2 , XM_015273047.1
    Accession Version XM_015273047.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product separin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008465.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015273047.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGAATCGCC TCAAAGGCAC CGACTTTGTG TCCCGCACTG CCAGCCCGGA GGGCACAGAG 
    GAGCTGCTCA GGGAGCTGTT GCAATGCCTC TCCAGCAGCA CGGTGGTGCC GGGCAGTGTG
    CAGAGCACGG TGTGCGACCG CGTCCTGCGG GCCTGCGTGC AGCGCCTGGC GGAGCCCGGC
    TGCGGCCTCA CGCACACCGA CGCGGTGCTG GCGTTGGCCG AGGCGGCCTG CAGGGGCTTC
    GTCACCGCCG CCACCGCCCC GCCGAGCCCT CTCTACCTGG AGAAGATCCT CTACTACCTG
    CTGAAGAACG CGGCTGCCCG CGGCCATCGC AGTGCCTGCA GGCGGGCGGC GGAGCTCCTC
    CACGCTCAGC TCTGCGCCCA CCAGCAGAGC CACGCGCCGT CCGCCGACTT CGGTGCTGTT
    GTCCATAGCA GCTTCTGCAC GCTGTGGGGC GCCGCGGGGT CGCTGGGGGA CCGCCTGCAG
    GCCCTGCGCT TCCTGCTGCT GCTGGAGGAC GAGGAGGGGG CCGTGCTGCC CCTGGAGCCG
    CCCTTCTTCA CCTCGCAGAC CGCCCGGCAG GCGGTGTTGG CCGCCACTCT CTCCAAAGCC
    CACCACGCTC CATCCTCGGC TTGCTTTGGC CGGGAGCTGA GGGGTATCCT GACCTCCCTG
    CGCGAGGGCG CGGCGCGGCC CCCCTCCCTG CCCCACGCCC TCTGCTTCTT GGAGCTGGCG
    TTGGAGCAGT GCCGGCAGCT CTGCCAGAGC GGCTGCCACG GGGAGGCGGA GGCGGCGGTG
    AGGGATGCGC GAGGATTCGT GGGGGGCTCT GAGGCGTTCG GCGAGGCCCT GGCCCTGCTG
    GAGGCCGGCG TGCAGCTGAG CCGGGCGCTG GCCCCGAACG GGGGCTCAGC AGGGCCGGCC
    CTGGCGCAGG CGGCGGCAGC GCTGGGCGGC GCAGCGAAGG CCTCGGGGCG CTTCCTCAGG
    GTGCTGGCCG AGGGCTGCCA GTTCGTCACC GCGTCCCTCC ACCAGCGTGC CAGGAGCAGC
    CAGCAGCAGC CGATGCCTCG GGACGACGTG CTCCAGCTCT GCGCCTTTGT GCGGGGGCAC
    TGCCGTGTCC TCGGCCTGCT GCTGCGCGTG GCGCCTCCTG ACGGCGTGAA GCAGCGGCTC
    GTGCTGAAGC AGCTGCTGTA CCACAGCCTC CAGCTCTTCA CCAGCGTGGC CTGTGACACC
    TTCCGCTCCT CCCAGGTGGC CGGGTGGTCA GGGCAGGAAC GGCTGACATT GGGCTGCCAG
    GACACCGTGA TGCAGATGCT GGAGGCTCTG GAGGGACTTC CCCAGAGGGA GCAAGCCAAG
    TACCTCGACA TTACCGCATC GTGCACGTTT AAGCTGGCAT ACATCTTCTA CAGCCAGAAC
    CTTTACAAGG AAGCCAGCTC CGTCTCCGAG CTCTTCTGCA AGAGGCTGAA GCAAATAGAT
    GCCTTCAGAT TTCCAGAGAT ACCTCCCGAA AAGCTGCACA GGTGCTTCAG GCTGCAGGTG
    GAAAGCTACC AAAAGCTGGG CCTGCTGGAG AGCGCGCTGG CGTCCTGCGT GCAGTGGCTG
    ACGTCGCTGC GGGGCCGCGT TGGGGAGCTG CTGGCGGAGC CCATCGGCCT CTGGGCCCGA
    GTGAAAATGG ACAGCGTGAA GCAGGGAGCG GAGGACTTAC GGCTGAGAAC CCTGAAGGAA
    GAACTGTCTG CTCACCACCT GGACGCGGAC ACCTTGGTGG CTGTGCTCTT TGCCGAGCTG
    AAGGCTTACA AGAGCATCCG TGCCGACACA GGGCAGGAAC GCTACAACGT GCTCTGCGAC
    CTGCTGGAGA TCTGCTCCGA GGACAGCGGC CGCCTGCATG AGCGCGCTGC CTGCTTGGTG
    GAGCTGGCCC AGGTCCTTTG CTACCACAAC TATGCCCAGC AAACCGACTG CTCCGCGTTG
    GATTCTGTCC ACGAAGCACT GCGGCTGTTG GATTCGGTGC CCCGGAGTGC TGAGAACCGG
    GAGCAGCTCC TCGATGACCG GGCGCAGGCT CTGCTCTGGC TCTACATCTG CACCCTGGAG
    TCCAAGCTGG AGGAGGGCAT AGCAAGGGAG CAGAGAGTCA AAGCTCTGGG ACAGAAGAGC
    CTGGAAGACT TTGAGCCCAA TGACCTCAAC TATGAAAGCA GGCTGCAGGA GGATGCATTC
    CTGAACAGCG GCATCTCCTT CAACCTGCTG ACAGAATTGG TGGTGGTGCT GGTGGTGGTT
    CAGTGCTGTG GCGAAGCTGC TCAGCCCCGT GCTGTGCCCA CAGCTCAGTC CAAGAGCCTG
    GACGATGCCT TAGCCCTATG GAAGCAGCTG CTGGCATCTC AGGGCATCCC AGCTGTGCGC
    AGTGTGGAGC AGACCACGGC CTCTCTTCGT GTTACAGCAG CTCTCTACAG GCTGATGGAC
    AAGCCTCTGC AAGCCATGGA AAGCTACCTG CTGGTGCGAG CCCTGTGCAG CGCACTTGGG
    GACAATTTGG GCACGGCCGG AGCTCTCTGC CAGGTCACCA AACTGCTCCT CCAGCTGGAA
    TGCCCCAGCT ACGCCAAGCT CTTCCTGGAA GAGGCAGAGT CCTGCTTGCA GAGAACAGAC
    GGGGGCAGCG ACTCCTACCT GCTGCTGCAG CAGACCTGCC TCGTGCTCCG CAGCCAGCTG
    TACTGCGCCA GCCACCGCAT TAAAGATGGC ACTGCCCTCT TGCTGGAGGT GCTCCAGAAC
    CCGGCTCTGC AGAAGACTGC CAAGGTCTGG TACATGCTCC AAGCACAAGT CCTGCAACTG
    ACCGCCACCT ACCTGAGCCT GCCGCCCAGC CACCTCTCGC CAGAGTTCAG GCAGTACATC
    TTCACGCAGG GGTGGAAGAC TCCCGAGACA GCTCTGAGCG AAGCCCACAG GTTGTTACGT
    GGCATCGTCC TCCTCCTGAT GGGCAGCAAC GTGCTGGGCT CCCACAAGTC AGCAGCAGAT
    ATCCGGTTCA TAGATTGTGG GGACAACGTA CTGCAGAAGT GGCAAGTGCT GGCCGACTTG
    CTGGGCTGCT CAGAGCACCT GGTGGTCCTC CTCAGCAGGG TGGAGGTGAT GTGCAAAGCC
    AAAGCCTTCT GCTTGGAGGC CATCAAGCTG GCTGTGAAAC TGCAGGCGGT CCGATGGTGT
    GCATCCTTCC TGGTGCTGAA GGCTCAGCTG GAGCTGCAGC AGAGTGAGCT GGAGCTGAGC
    CACTGGAACC TGCAGCAGGC TCTGTTCCTC CTGGAGTCCG GCACCGAGTT TGAGGCTGAT
    GAGAAGCAGC GAGCCCCCCT GAAGATCTTG CCCAGGAAAG GCAAACCAGA GGGCAGGAAG
    CAGCGGGATG AGGGCTCTGA GCTCCCGGGA GAAGATGAGG GCTTTCTGAA GGGCCCTGCG
    CTGGAGTTTG TGGCCATGGT GAGCGGCCTG GAGAAAGCAG ATGACCTCAG CTCCTCGCCA
    CAGCTGAAAC CCAAGAGGAA GAGGAGGCTC GCCTTCCTCG CCCACCCCGC TGCCTGCCCC
    TGCTGCCTCT GCTCCGACCT GACCCTGTCG TCCCTCTGCC TCCGCTGGCT GCTCTGCTGT
    GCGCGGGGTG AGCTGGCGGC CGGCAGTGCA GCCGAAGGGC TGGGGCTGAT CCAAGCCACC
    CTGCAGCGCT GCGGCACCGT GGGCCCTCGC TTCACCGTCC TGCTGCAGGA CAAGCTGGGC
    AGTGGTCACA AGGCACCTGC ACTGCGGCTG TTAGATGATT TGGTGGCCAC TGGCTATGCC
    GCACTGGCCC TTCACAGCCT GGCTGGTCTC TGTCCTGAGG AGAAGCTGGA GGAGCACCTG
    AAAGCCGGTC TGTCTTTCCT GGCATCCTGC AGCCTCCACC TGCCCAGCCT GGGGGTCTCC
    AAGGCCAGCC TGCTGCTTGC CAAAGCCACG GCTGCTGTTT GCCGCTTGGC CGCCAAGCAC
    GGAGGCTCTG TGGACGCTGT CTTTGCCTCT GCTTGGGCTT GGCAGCCAGC CCCACTGAGC
    CCCACCGAGC CCAAAGCAGC AGCGGCTGCG CCCAGGATGG ACAAAGCTCA ACTAAAGAGG
    CTTAAAAACA AAGCGGCAAC CACAGCAGCC CCTGTGCTCA AACCCAAAGC CAAGGAGACC
    CAGAGAGCAA AGCCCCCCTC GGTGGGAGAT ACCAACGATG CCTTTCTCGT GACCGATTCC
    GACACAGAGC CGCCCGCTGC TGTTGGTCAG ACTGCGACAA AGTCCTGCAC CACACGCCCC
    AATGCCCGTC CCTGCAGCAA AGCACGTGCA CAGGGCTCTG TGGCATCCCC TGCACGGAAA
    CCCAGAGGGA AGAAGAAGCA GCAAGCCAAG AGCACAGACA CCGACGATGC CTTCGCTTTG
    GGTGACTCGG ACAGCAAGGT GCCTCCCATT GTGGTCCGGC CGGCAGCAGT GCCCTGCACT
    CCGCGCCAGA AGCCGTCCTG TCCTCTAGCC AAGACATACA CCCGGGGGGC TGCGATGTCC
    CTACGGCCCA GGGCTCCCTT CACCGTGTTC AGCGAGGCCT CGCCACCACT CAGCAAGTCA
    CAGCTCCTGA AAGCACCCAA AGCCTGTGGG AAAACCAGAT CCCGGCTGCC GGTGACATTC
    AGTGATGACA GCGACACCGA GGGTCTCAAA TCAGTGCTGA GCCCCATTGC TGGCAAAGCA
    CCCATTGCCG GCTGCAAGAC GCCGAAGTCA GTGTGCTGCT CAAAGAGAGC TCTGCTCCCC
    CGGTCCTTGG AGATGGGGGG CTCCCAGCTG AGCTCCTCCT CCGTGGGCAG AGGCTGTAGG
    AGCAGCACCC GGCCCCGGCG GGGGCGCACA GCTGGGGGCA CCGAGAAGAA GGAGCGGGCA
    GCACGCAGGG CTCCGAGCTG GAAAGGGGAT GAGGAACGGG AGCTGCTGTG TGCCATCGAA
    GAGAAGGAAA AGGTGGAGGA GCAGGTCAAC GTCAGCTTGG AGCTACTCCA AGCCTCTGAG
    GATGAAGCCC AGGGTGGAAT CCAACGCAGG CTGGAGGGCG CGGACATGGA GCTCGAGGGG
    CTGCGGCAGG AGGTTGGTGG AGAGGTCCTG GCACGGCAGA GGCTGGGCAG TACGGAGCCC
    CTGTGCCTCA GCGCCCTGCA CAACGTGCTG CCAGCTGTGG ATGACGCCTC CTTGCTGGAC
    ACTGCCTGCG GGCTCCTGAA ATCTGCCTTC AGCTGCATCA GCCACTGCCC ACCCGGCACG
    CTGTACAGCC AGCTCTGTCA GCTCCTGGCT CTGGCCACGG GTGATCAGGA CCCCATCACC
    ACTGCCTACC TGCTGGCTGA GTCTGTCTCG GTCACCACGC GCCATCAGCT GCTCAGTATC
    ATCCACAAGA AGCTTCATAA GGAGAAGAAA GCAGCAGCAG ATGTGGCTGA GCAGCTCCAA
    GGGCTGACCC TGCAGGATAA GGATGCTCCT TCACACCACT GCCACCTCAC TGAGCTCCAG
    GACCTCTTCA GCTTCAGCCC CACGGGTGTG GAGCCCCAGG AGTGCACCAG CTTCAGTGAG
    CAGCTGCAGC AGATCCCAGC TGGGGTGACT GTGTGCGTGC TGACCCTGGT GAGCATCCAG
    CCCACCTCCG TGGGGGACAC ACTGCTGCTG ACACGGTTGG AGAGGGGCAG CGCTCCCATC
    ACTGTCCGCA TCCCAACTGC GCACACCAAG ACCCCGCTGA GCTCAGTGCT GAGTGACTTC
    GACTCCATCC AGAAGGAGCA GAAGGATGCC AACGGCTACA CTGAAAAGAG AGCCTGGTGG
    CTGTGCCGCT TCGAGCTGGA CCAGAGGATG AAGAACCTCA TTGAGACGCT GGAAATGCAG
    GTGCTGGGTT GCTGGAAGGC AGCGCTGCTC CCTGCAGCCC AGGATCCCCT GCTGGCTGAG
    GAAGCTGCCC GTTTATGTGC TCAGCTGCAG CAGTGTGGCT GGTGGGGCAA AGATCCGACC
    CTACTCAAGG TGCTGCTGAA CGCCGCTGCC CTCCTCAGCC CTGCTGATGT GCAAGCCCTG
    GCCGTGGGGC TGTGCCCAGC TCAGCCCAGC AGAGCCCGGC TCCTCTTGCA GGAGGCTCTG
    GACAAGCAGA AAAACAGCAC ACGGGAGCCG GGCGGTTCCG TCATCCTGGT GCTGGATAAG
    CACCTGCAGA AGCTGCCCTG GGAGAGCATG GCGTGCCTGA GGGACCTGCC CGTCACCCGG
    CTCCCATCCC TGCGCTTCCT CCTCAGCTAC ACCCTGACAC AGAGGCAGAC AAACTCCGTG
    CTGTGCCGCG GCATTGACCC CAGCAGGACC TTCTATGTCC TCAACCCTCA GAACAACCTC
    TCGAGCACTG AGGAGTGTTT CCGCAGCTGG TTTGAGAGTG AACCAGGCTG GAGAGGGGTG
    ACAGGATCAG TCCCCAGCCA GGAGCAGATG CAGGCAGCGC TGCTGGAGCA CGATCTCTAC
    ATCTACGCGG GACACGGAGC CGGTGCCCAC CTGCTGGACG GGCAGACCAT CTCCCAGCTG
    CAGTGCCGTG CTGTCGCTCT GCTCTTCGGC TGCAGCAGCG CCGCCCTCGC CGTGCGTGGC
    AGCCTGGAGG GTACGGGCAT TGTGCTGAAG TACATCATGG CTGGCTGCCC CCTCGTCTTG
    GGCAACCTGT GGGACGTCAC CGACCGCGAC ATTGACCGCT ATGCTGAGGC ACTGCTGCGT
    GCCTGGCTGG GGGCTCCTCG GCCCGGAGCC CCCCTTCTCC ACTACATGGC CGAGGCCCGG
    CGTGCCCCCC GCCTCCAGCA CCTCATCGGG GCCGCCCCCG TGGCCTATGG GCTCCCTGTG
    GCCCTGCAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015128533.1
    CDS567..7298
    Translation

    Target ORF information:

    RefSeq Version XM_015273047.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015273047.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGAATCGCC TCAAAGGCAC CGACTTTGTG TCCCGCACTG CCAGCCCGGA GGGCACAGAG 
    GAGCTGCTCA GGGAGCTGTT GCAATGCCTC TCCAGCAGCA CGGTGGTGCC GGGCAGTGTG
    CAGAGCACGG TGTGCGACCG CGTCCTGCGG GCCTGCGTGC AGCGCCTGGC GGAGCCCGGC
    TGCGGCCTCA CGCACACCGA CGCGGTGCTG GCGTTGGCCG AGGCGGCCTG CAGGGGCTTC
    GTCACCGCCG CCACCGCCCC GCCGAGCCCT CTCTACCTGG AGAAGATCCT CTACTACCTG
    CTGAAGAACG CGGCTGCCCG CGGCCATCGC AGTGCCTGCA GGCGGGCGGC GGAGCTCCTC
    CACGCTCAGC TCTGCGCCCA CCAGCAGAGC CACGCGCCGT CCGCCGACTT CGGTGCTGTT
    GTCCATAGCA GCTTCTGCAC GCTGTGGGGC GCCGCGGGGT CGCTGGGGGA CCGCCTGCAG
    GCCCTGCGCT TCCTGCTGCT GCTGGAGGAC GAGGAGGGGG CCGTGCTGCC CCTGGAGCCG
    CCCTTCTTCA CCTCGCAGAC CGCCCGGCAG GCGGTGTTGG CCGCCACTCT CTCCAAAGCC
    CACCACGCTC CATCCTCGGC TTGCTTTGGC CGGGAGCTGA GGGGTATCCT GACCTCCCTG
    CGCGAGGGCG CGGCGCGGCC CCCCTCCCTG CCCCACGCCC TCTGCTTCTT GGAGCTGGCG
    TTGGAGCAGT GCCGGCAGCT CTGCCAGAGC GGCTGCCACG GGGAGGCGGA GGCGGCGGTG
    AGGGATGCGC GAGGATTCGT GGGGGGCTCT GAGGCGTTCG GCGAGGCCCT GGCCCTGCTG
    GAGGCCGGCG TGCAGCTGAG CCGGGCGCTG GCCCCGAACG GGGGCTCAGC AGGGCCGGCC
    CTGGCGCAGG CGGCGGCAGC GCTGGGCGGC GCAGCGAAGG CCTCGGGGCG CTTCCTCAGG
    GTGCTGGCCG AGGGCTGCCA GTTCGTCACC GCGTCCCTCC ACCAGCGTGC CAGGAGCAGC
    CAGCAGCAGC CGATGCCTCG GGACGACGTG CTCCAGCTCT GCGCCTTTGT GCGGGGGCAC
    TGCCGTGTCC TCGGCCTGCT GCTGCGCGTG GCGCCTCCTG ACGGCGTGAA GCAGCGGCTC
    GTGCTGAAGC AGCTGCTGTA CCACAGCCTC CAGCTCTTCA CCAGCGTGGC CTGTGACACC
    TTCCGCTCCT CCCAGGTGGC CGGGTGGTCA GGGCAGGAAC GGCTGACATT GGGCTGCCAG
    GACACCGTGA TGCAGATGCT GGAGGCTCTG GAGGGACTTC CCCAGAGGGA GCAAGCCAAG
    TACCTCGACA TTACCGCATC GTGCACGTTT AAGCTGGCAT ACATCTTCTA CAGCCAGAAC
    CTTTACAAGG AAGCCAGCTC CGTCTCCGAG CTCTTCTGCA AGAGGCTGAA GCAAATAGAT
    GCCTTCAGAT TTCCAGAGAT ACCTCCCGAA AAGCTGCACA GGTGCTTCAG GCTGCAGGTG
    GAAAGCTACC AAAAGCTGGG CCTGCTGGAG AGCGCGCTGG CGTCCTGCGT GCAGTGGCTG
    ACGTCGCTGC GGGGCCGCGT TGGGGAGCTG CTGGCGGAGC CCATCGGCCT CTGGGCCCGA
    GTGAAAATGG ACAGCGTGAA GCAGGGAGCG GAGGACTTAC GGCTGAGAAC CCTGAAGGAA
    GAACTGTCTG CTCACCACCT GGACGCGGAC ACCTTGGTGG CTGTGCTCTT TGCCGAGCTG
    AAGGCTTACA AGAGCATCCG TGCCGACACA GGGCAGGAAC GCTACAACGT GCTCTGCGAC
    CTGCTGGAGA TCTGCTCCGA GGACAGCGGC CGCCTGCATG AGCGCGCTGC CTGCTTGGTG
    GAGCTGGCCC AGGTCCTTTG CTACCACAAC TATGCCCAGC AAACCGACTG CTCCGCGTTG
    GATTCTGTCC ACGAAGCACT GCGGCTGTTG GATTCGGTGC CCCGGAGTGC TGAGAACCGG
    GAGCAGCTCC TCGATGACCG GGCGCAGGCT CTGCTCTGGC TCTACATCTG CACCCTGGAG
    TCCAAGCTGG AGGAGGGCAT AGCAAGGGAG CAGAGAGTCA AAGCTCTGGG ACAGAAGAGC
    CTGGAAGACT TTGAGCCCAA TGACCTCAAC TATGAAAGCA GGCTGCAGGA GGATGCATTC
    CTGAACAGCG GCATCTCCTT CAACCTGCTG ACAGAATTGG TGGTGGTGCT GGTGGTGGTT
    CAGTGCTGTG GCGAAGCTGC TCAGCCCCGT GCTGTGCCCA CAGCTCAGTC CAAGAGCCTG
    GACGATGCCT TAGCCCTATG GAAGCAGCTG CTGGCATCTC AGGGCATCCC AGCTGTGCGC
    AGTGTGGAGC AGACCACGGC CTCTCTTCGT GTTACAGCAG CTCTCTACAG GCTGATGGAC
    AAGCCTCTGC AAGCCATGGA AAGCTACCTG CTGGTGCGAG CCCTGTGCAG CGCACTTGGG
    GACAATTTGG GCACGGCCGG AGCTCTCTGC CAGGTCACCA AACTGCTCCT CCAGCTGGAA
    TGCCCCAGCT ACGCCAAGCT CTTCCTGGAA GAGGCAGAGT CCTGCTTGCA GAGAACAGAC
    GGGGGCAGCG ACTCCTACCT GCTGCTGCAG CAGACCTGCC TCGTGCTCCG CAGCCAGCTG
    TACTGCGCCA GCCACCGCAT TAAAGATGGC ACTGCCCTCT TGCTGGAGGT GCTCCAGAAC
    CCGGCTCTGC AGAAGACTGC CAAGGTCTGG TACATGCTCC AAGCACAAGT CCTGCAACTG
    ACCGCCACCT ACCTGAGCCT GCCGCCCAGC CACCTCTCGC CAGAGTTCAG GCAGTACATC
    TTCACGCAGG GGTGGAAGAC TCCCGAGACA GCTCTGAGCG AAGCCCACAG GTTGTTACGT
    GGCATCGTCC TCCTCCTGAT GGGCAGCAAC GTGCTGGGCT CCCACAAGTC AGCAGCAGAT
    ATCCGGTTCA TAGATTGTGG GGACAACGTA CTGCAGAAGT GGCAAGTGCT GGCCGACTTG
    CTGGGCTGCT CAGAGCACCT GGTGGTCCTC CTCAGCAGGG TGGAGGTGAT GTGCAAAGCC
    AAAGCCTTCT GCTTGGAGGC CATCAAGCTG GCTGTGAAAC TGCAGGCGGT CCGATGGTGT
    GCATCCTTCC TGGTGCTGAA GGCTCAGCTG GAGCTGCAGC AGAGTGAGCT GGAGCTGAGC
    CACTGGAACC TGCAGCAGGC TCTGTTCCTC CTGGAGTCCG GCACCGAGTT TGAGGCTGAT
    GAGAAGCAGC GAGCCCCCCT GAAGATCTTG CCCAGGAAAG GCAAACCAGA GGGCAGGAAG
    CAGCGGGATG AGGGCTCTGA GCTCCCGGGA GAAGATGAGG GCTTTCTGAA GGGCCCTGCG
    CTGGAGTTTG TGGCCATGGT GAGCGGCCTG GAGAAAGCAG ATGACCTCAG CTCCTCGCCA
    CAGCTGAAAC CCAAGAGGAA GAGGAGGCTC GCCTTCCTCG CCCACCCCGC TGCCTGCCCC
    TGCTGCCTCT GCTCCGACCT GACCCTGTCG TCCCTCTGCC TCCGCTGGCT GCTCTGCTGT
    GCGCGGGGTG AGCTGGCGGC CGGCAGTGCA GCCGAAGGGC TGGGGCTGAT CCAAGCCACC
    CTGCAGCGCT GCGGCACCGT GGGCCCTCGC TTCACCGTCC TGCTGCAGGA CAAGCTGGGC
    AGTGGTCACA AGGCACCTGC ACTGCGGCTG TTAGATGATT TGGTGGCCAC TGGCTATGCC
    GCACTGGCCC TTCACAGCCT GGCTGGTCTC TGTCCTGAGG AGAAGCTGGA GGAGCACCTG
    AAAGCCGGTC TGTCTTTCCT GGCATCCTGC AGCCTCCACC TGCCCAGCCT GGGGGTCTCC
    AAGGCCAGCC TGCTGCTTGC CAAAGCCACG GCTGCTGTTT GCCGCTTGGC CGCCAAGCAC
    GGAGGCTCTG TGGACGCTGT CTTTGCCTCT GCTTGGGCTT GGCAGCCAGC CCCACTGAGC
    CCCACCGAGC CCAAAGCAGC AGCGGCTGCG CCCAGGATGG ACAAAGCTCA ACTAAAGAGG
    CTTAAAAACA AAGCGGCAAC CACAGCAGCC CCTGTGCTCA AACCCAAAGC CAAGGAGACC
    CAGAGAGCAA AGCCCCCCTC GGTGGGAGAT ACCAACGATG CCTTTCTCGT GACCGATTCC
    GACACAGAGC CGCCCGCTGC TGTTGGTCAG ACTGCGACAA AGTCCTGCAC CACACGCCCC
    AATGCCCGTC CCTGCAGCAA AGCACGTGCA CAGGGCTCTG TGGCATCCCC TGCACGGAAA
    CCCAGAGGGA AGAAGAAGCA GCAAGCCAAG AGCACAGACA CCGACGATGC CTTCGCTTTG
    GGTGACTCGG ACAGCAAGGT GCCTCCCATT GTGGTCCGGC CGGCAGCAGT GCCCTGCACT
    CCGCGCCAGA AGCCGTCCTG TCCTCTAGCC AAGACATACA CCCGGGGGGC TGCGATGTCC
    CTACGGCCCA GGGCTCCCTT CACCGTGTTC AGCGAGGCCT CGCCACCACT CAGCAAGTCA
    CAGCTCCTGA AAGCACCCAA AGCCTGTGGG AAAACCAGAT CCCGGCTGCC GGTGACATTC
    AGTGATGACA GCGACACCGA GGGTCTCAAA TCAGTGCTGA GCCCCATTGC TGGCAAAGCA
    CCCATTGCCG GCTGCAAGAC GCCGAAGTCA GTGTGCTGCT CAAAGAGAGC TCTGCTCCCC
    CGGTCCTTGG AGATGGGGGG CTCCCAGCTG AGCTCCTCCT CCGTGGGCAG AGGCTGTAGG
    AGCAGCACCC GGCCCCGGCG GGGGCGCACA GCTGGGGGCA CCGAGAAGAA GGAGCGGGCA
    GCACGCAGGG CTCCGAGCTG GAAAGGGGAT GAGGAACGGG AGCTGCTGTG TGCCATCGAA
    GAGAAGGAAA AGGTGGAGGA GCAGGTCAAC GTCAGCTTGG AGCTACTCCA AGCCTCTGAG
    GATGAAGCCC AGGGTGGAAT CCAACGCAGG CTGGAGGGCG CGGACATGGA GCTCGAGGGG
    CTGCGGCAGG AGGTTGGTGG AGAGGTCCTG GCACGGCAGA GGCTGGGCAG TACGGAGCCC
    CTGTGCCTCA GCGCCCTGCA CAACGTGCTG CCAGCTGTGG ATGACGCCTC CTTGCTGGAC
    ACTGCCTGCG GGCTCCTGAA ATCTGCCTTC AGCTGCATCA GCCACTGCCC ACCCGGCACG
    CTGTACAGCC AGCTCTGTCA GCTCCTGGCT CTGGCCACGG GTGATCAGGA CCCCATCACC
    ACTGCCTACC TGCTGGCTGA GTCTGTCTCG GTCACCACGC GCCATCAGCT GCTCAGTATC
    ATCCACAAGA AGCTTCATAA GGAGAAGAAA GCAGCAGCAG ATGTGGCTGA GCAGCTCCAA
    GGGCTGACCC TGCAGGATAA GGATGCTCCT TCACACCACT GCCACCTCAC TGAGCTCCAG
    GACCTCTTCA GCTTCAGCCC CACGGGTGTG GAGCCCCAGG AGTGCACCAG CTTCAGTGAG
    CAGCTGCAGC AGATCCCAGC TGGGGTGACT GTGTGCGTGC TGACCCTGGT GAGCATCCAG
    CCCACCTCCG TGGGGGACAC ACTGCTGCTG ACACGGTTGG AGAGGGGCAG CGCTCCCATC
    ACTGTCCGCA TCCCAACTGC GCACACCAAG ACCCCGCTGA GCTCAGTGCT GAGTGACTTC
    GACTCCATCC AGAAGGAGCA GAAGGATGCC AACGGCTACA CTGAAAAGAG AGCCTGGTGG
    CTGTGCCGCT TCGAGCTGGA CCAGAGGATG AAGAACCTCA TTGAGACGCT GGAAATGCAG
    GTGCTGGGTT GCTGGAAGGC AGCGCTGCTC CCTGCAGCCC AGGATCCCCT GCTGGCTGAG
    GAAGCTGCCC GTTTATGTGC TCAGCTGCAG CAGTGTGGCT GGTGGGGCAA AGATCCGACC
    CTACTCAAGG TGCTGCTGAA CGCCGCTGCC CTCCTCAGCC CTGCTGATGT GCAAGCCCTG
    GCCGTGGGGC TGTGCCCAGC TCAGCCCAGC AGAGCCCGGC TCCTCTTGCA GGAGGCTCTG
    GACAAGCAGA AAAACAGCAC ACGGGAGCCG GGCGGTTCCG TCATCCTGGT GCTGGATAAG
    CACCTGCAGA AGCTGCCCTG GGAGAGCATG GCGTGCCTGA GGGACCTGCC CGTCACCCGG
    CTCCCATCCC TGCGCTTCCT CCTCAGCTAC ACCCTGACAC AGAGGCAGAC AAACTCCGTG
    CTGTGCCGCG GCATTGACCC CAGCAGGACC TTCTATGTCC TCAACCCTCA GAACAACCTC
    TCGAGCACTG AGGAGTGTTT CCGCAGCTGG TTTGAGAGTG AACCAGGCTG GAGAGGGGTG
    ACAGGATCAG TCCCCAGCCA GGAGCAGATG CAGGCAGCGC TGCTGGAGCA CGATCTCTAC
    ATCTACGCGG GACACGGAGC CGGTGCCCAC CTGCTGGACG GGCAGACCAT CTCCCAGCTG
    CAGTGCCGTG CTGTCGCTCT GCTCTTCGGC TGCAGCAGCG CCGCCCTCGC CGTGCGTGGC
    AGCCTGGAGG GTACGGGCAT TGTGCTGAAG TACATCATGG CTGGCTGCCC CCTCGTCTTG
    GGCAACCTGT GGGACGTCAC CGACCGCGAC ATTGACCGCT ATGCTGAGGC ACTGCTGCGT
    GCCTGGCTGG GGGCTCCTCG GCCCGGAGCC CCCCTTCTCC ACTACATGGC CGAGGCCCGG
    CGTGCCCCCC GCCTCCAGCA CCTCATCGGG GCCGCCCCCG TGGCCTATGG GCTCCCTGTG
    GCCCTGCAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa50164
    Clone ID Related Accession (Same CDS sequence) XM_015273047.2 , XM_015273047.1
    Accession Version XM_015273047.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product separin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_464144.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGAATCGCC TCAAAGGCAC CGACTTTGTG TCCCGCACTG CCAGCCCGGA GGGCACAGAG 
    GAGCTGCTCA GGGAGCTGTT GCAATGCCTC TCCAGCAGCA CGGTGGTGCC GGGCAGTGTG
    CAGAGCACGG TGTGCGACCG CGTCCTGCGG GCCTGCGTGC AGCGCCTGGC GGAGCCCGGC
    TGCGGCCTCA CGCACACCGA CGCGGTGCTG GCGTTGGCCG AGGCGGCCTG CAGGGGCTTC
    GTCACCGCCG CCACCGCCCC GCCGAGCCCT CTCTACCTGG AGAAGATCCT CTACTACCTG
    CTGAAGAACG CGGCTGCCCG CGGCCATCGC AGTGCCTGCA GGCGGGCGGC GGAGCTCCTC
    CACGCTCAGC TCTGCGCCCA CCAGCAGAGC CACGCGCCGT CCGCCGACTT CGGTGCTGTT
    GTCCATAGCA GCTTCTGCAC GCTGTGGGGC GCCGCGGGGT CGCTGGGGGA CCGCCTGCAG
    GCCCTGCGCT TCCTGCTGCT GCTGGAGGAC GAGGAGGGGG CCGTGCTGCC CCTGGAGCCG
    CCCTTCTTCA CCTCGCAGAC CGCCCGGCAG GCGGTGTTGG CCGCCACTCT CTCCAAAGCC
    CACCACGCTC CATCCTCGGC TTGCTTTGGC CGGGAGCTGA GGGGTATCCT GACCTCCCTG
    CGCGAGGGCG CGGCGCGGCC CCCCTCCCTG CCCCACGCCC TCTGCTTCTT GGAGCTGGCG
    TTGGAGCAGT GCCGGCAGCT CTGCCAGAGC GGCTGCCACG GGGAGGCGGA GGCGGCGGTG
    AGGGATGCGC GAGGATTCGT GGGGGGCTCT GAGGCGTTCG GCGAGGCCCT GGCCCTGCTG
    GAGGCCGGCG TGCAGCTGAG CCGGGCGCTG GCCCCGAACG GGGGCTCAGC AGGGCCGGCC
    CTGGCGCAGG CGGCGGCAGC GCTGGGCGGC GCAGCGAAGG CCTCGGGGCG CTTCCTCAGG
    GTGCTGGCCG AGGGCTGCCA GTTCGTCACC GCGTCCCTCC ACCAGCGTGC CAGGAGCAGC
    CAGCAGCAGC CGATGCCTCG GGACGACGTG CTCCAGCTCT GCGCCTTTGT GCGGGGGCAC
    TGCCGTGTCC TCGGCCTGCT GCTGCGCGTG GCGCCTCCTG ACGGCGTGAA GCAGCGGCTC
    GTGCTGAAGC AGCTGCTGTA CCACAGCCTC CAGCTCTTCA CCAGCGTGGC CTGTGACACC
    TTCCGCTCCT CCCAGGTGGC CGGGTGGTCA GGGCAGGAAC GGCTGACATT GGGCTGCCAG
    GACACCGTGA TGCAGATGCT GGAGGCTCTG GAGGGACTTC CCCAGAGGGA GCAAGCCAAG
    TACCTCGACA TTACCGCATC GTGCACGTTT AAGCTGGCAT ACATCTTCTA CAGCCAGAAC
    CTTTACAAGG AAGCCAGCTC CGTCTCCGAG CTCTTCTGCA AGAGGCTGAA GCAAATAGAT
    GCCTTCAGAT TTCCAGAGAT ACCTCCCGAA AAGCTGCACA GGTGCTTCAG GCTGCAGGTG
    GAAAGCTACC AAAAGCTGGG CCTGCTGGAG AGCGCGCTGG CGTCCTGCGT GCAGTGGCTG
    ACGTCGCTGC GGGGCCGCGT TGGGGAGCTG CTGGCGGAGC CCATCGGCCT CTGGGCCCGA
    GTGAAAATGG ACAGCGTGAA GCAGGGAGCG GAGGACTTAC GGCTGAGAAC CCTGAAGGAA
    GAACTGTCTG CTCACCACCT GGACGCGGAC ACCTTGGTGG CTGTGCTCTT TGCCGAGCTG
    AAGGCTTACA AGAGCATCCG TGCCGACACA GGGCAGGAAC GCTACAACGT GCTCTGCGAC
    CTGCTGGAGA TCTGCTCCGA GGACAGCGGC CGCCTGCATG AGCGCGCTGC CTGCTTGGTG
    GAGCTGGCCC AGGTCCTTTG CTACCACAAC TATGCCCAGC AAACCGACTG CTCCGCGTTG
    GATTCTGTCC ACGAAGCACT GCGGCTGTTG GATTCGGTGC CCCGGAGTGC TGAGAACCGG
    GAGCAGCTCC TCGATGACCG GGCGCAGGCT CTGCTCTGGC TCTACATCTG CACCCTGGAG
    TCCAAGCTGG AGGAGGGCAT AGCAAGGGAG CAGAGAGTCA AAGCTCTGGG ACAGAAGAGC
    CTGGAAGACT TTGAGCCCAA TGACCTCAAC TATGAAAGCA GGCTGCAGGA GGATGCATTC
    CTGAACAGCG GCATCTCCTT CAACCTGCTG ACAGAATTGG TGGTGGTGCT GGTGGTGGTT
    CAGTGCTGTG GCGAAGCTGC TCAGCCCCGT GCTGTGCCCA CAGCTCAGTC CAAGAGCCTG
    GACGATGCCT TAGCCCTATG GAAGCAGCTG CTGGCATCTC AGGGCATCCC AGCTGTGCGC
    AGTGTGGAGC AGACCACGGC CTCTCTTCGT GTTACAGCAG CTCTCTACAG GCTGATGGAC
    AAGCCTCTGC AAGCCATGGA AAGCTACCTG CTGGTGCGAG CCCTGTGCAG CGCACTTGGG
    GACAATTTGG GCACGGCCGG AGCTCTCTGC CAGGTCACCA AACTGCTCCT CCAGCTGGAA
    TGCCCCAGCT ACGCCAAGCT CTTCCTGGAA GAGGCAGAGT CCTGCTTGCA GAGAACAGAC
    GGGGGCAGCG ACTCCTACCT GCTGCTGCAG CAGACCTGCC TCGTGCTCCG CAGCCAGCTG
    TACTGCGCCA GCCACCGCAT TAAAGATGGC ACTGCCCTCT TGCTGGAGGT GCTCCAGAAC
    CCGGCTCTGC AGAAGACTGC CAAGGTCTGG TACATGCTCC AAGCACAAGT CCTGCAACTG
    ACCGCCACCT ACCTGAGCCT GCCGCCCAGC CACCTCTCGC CAGAGTTCAG GCAGTACATC
    TTCACGCAGG GGTGGAAGAC TCCCGAGACA GCTCTGAGCG AAGCCCACAG GTTGTTACGT
    GGCATCGTCC TCCTCCTGAT GGGCAGCAAC GTGCTGGGCT CCCACAAGTC AGCAGCAGAT
    ATCCGGTTCA TAGATTGTGG GGACAACGTA CTGCAGAAGT GGCAAGTGCT GGCCGACTTG
    CTGGGCTGCT CAGAGCACCT GGTGGTCCTC CTCAGCAGGG TGGAGGTGAT GTGCAAAGCC
    AAAGCCTTCT GCTTGGAGGC CATCAAGCTG GCTGTGAAAC TGCAGGCGGT CCGATGGTGT
    GCATCCTTCC TGGTGCTGAA GGCTCAGCTG GAGCTGCAGC AGAGTGAGCT GGAGCTGAGC
    CACTGGAACC TGCAGCAGGC TCTGTTCCTC CTGGAGTCCG GCACCGAGTT TGAGGCTGAT
    GAGAAGCAGC GAGCCCCCCT GAAGATCTTG CCCAGGAAAG GCAAACCAGA GGGCAGGAAG
    CAGCGGGATG AGGGCTCTGA GCTCCCGGGA GAAGATGAGG GCTTTCTGAA GGGCCCTGCG
    CTGGAGTTTG TGGCCATGGT GAGCGGCCTG GAGAAAGCAG ATGACCTCAG CTCCTCGCCA
    CAGCTGAAAC CCAAGAGGAA GAGGAGGCTC GCCTTCCTCG CCCACCCCGC TGCCTGCCCC
    TGCTGCCTCT GCTCCGACCT GACCCTGTCG TCCCTCTGCC TCCGCTGGCT GCTCTGCTGT
    GCGCGGGGTG AGCTGGCGGC CGGCAGTGCA GCCGAAGGGC TGGGGCTGAT CCAAGCCACC
    CTGCAGCGCT GCGGCACCGT GGGCCCTCGC TTCACCGTCC TGCTGCAGGA CAAGCTGGGC
    AGTGGTCACA AGGCACCTGC ACTGCGGCTG TTAGATGATT TGGTGGCCAC TGGCTATGCC
    GCACTGGCCC TTCACAGCCT GGCTGGTCTC TGTCCTGAGG AGAAGCTGGA GGAGCACCTG
    AAAGCCGGTC TGTCTTTCCT GGCATCCTGC AGCCTCCACC TGCCCAGCCT GGGGGTCTCC
    AAGGCCAGCC TGCTGCTTGC CAAAGCCACG GCTGCTGTTT GCCGCTTGGC CGCCAAGCAC
    GGAGGCTCTG TGGACGCTGT CTTTGCCTCT GCTTGGGCTT GGCAGCCAGC CCCACTGAGC
    CCCACCGAGC CCAAAGCAGC AGCGGCTGCG CCCAGGATGG ACAAAGCTCA ACTAAAGAGG
    CTTAAAAACA AAGCGGCAAC CACAGCAGCC CCTGTGCTCA AACCCAAAGC CAAGGAGACC
    CAGAGAGCAA AGCCCCCCTC GGTGGGAGAT ACCAACGATG CCTTTCTCGT GACCGATTCC
    GACACAGAGC CGCCCGCTGC TGTTGGTCAG ACTGCGACAA AGTCCTGCAC CACACGCCCC
    AATGCCCGTC CCTGCAGCAA AGCACGTGCA CAGGGCTCTG TGGCATCCCC TGCACGGAAA
    CCCAGAGGGA AGAAGAAGCA GCAAGCCAAG AGCACAGACA CCGACGATGC CTTCGCTTTG
    GGTGACTCGG ACAGCAAGGT GCCTCCCATT GTGGTCCGGC CGGCAGCAGT GCCCTGCACT
    CCGCGCCAGA AGCCGTCCTG TCCTCTAGCC AAGACATACA CCCGGGGGGC TGCGATGTCC
    CTACGGCCCA GGGCTCCCTT CACCGTGTTC AGCGAGGCCT CGCCACCACT CAGCAAGTCA
    CAGCTCCTGA AAGCACCCAA AGCCTGTGGG AAAACCAGAT CCCGGCTGCC GGTGACATTC
    AGTGATGACA GCGACACCGA GGGTCTCAAA TCAGTGCTGA GCCCCATTGC TGGCAAAGCA
    CCCATTGCCG GCTGCAAGAC GCCGAAGTCA GTGTGCTGCT CAAAGAGAGC TCTGCTCCCC
    CGGTCCTTGG AGATGGGGGG CTCCCAGCTG AGCTCCTCCT CCGTGGGCAG AGGCTGTAGG
    AGCAGCACCC GGCCCCGGCG GGGGCGCACA GCTGGGGGCA CCGAGAAGAA GGAGCGGGCA
    GCACGCAGGG CTCCGAGCTG GAAAGGGGAT GAGGAACGGG AGCTGCTGTG TGCCATCGAA
    GAGAAGGAAA AGGTGGAGGA GCAGGTCAAC GTCAGCTTGG AGCTACTCCA AGCCTCTGAG
    GATGAAGCCC AGGGTGGAAT CCAACGCAGG CTGGAGGGCG CGGACATGGA GCTCGAGGGG
    CTGCGGCAGG AGGTTGGTGG AGAGGTCCTG GCACGGCAGA GGCTGGGCAG TACGGAGCCC
    CTGTGCCTCA GCGCCCTGCA CAACGTGCTG CCAGCTGTGG ATGACGCCTC CTTGCTGGAC
    ACTGCCTGCG GGCTCCTGAA ATCTGCCTTC AGCTGCATCA GCCACTGCCC ACCCGGCACG
    CTGTACAGCC AGCTCTGTCA GCTCCTGGCT CTGGCCACGG GTGATCAGGA CCCCATCACC
    ACTGCCTACC TGCTGGCTGA GTCTGTCTCG GTCACCACGC GCCATCAGCT GCTCAGTATC
    ATCCACAAGA AGCTTCATAA GGAGAAGAAA GCAGCAGCAG ATGTGGCTGA GCAGCTCCAA
    GGGCTGACCC TGCAGGATAA GGATGCTCCT TCACACCACT GCCACCTCAC TGAGCTCCAG
    GACCTCTTCA GCTTCAGCCC CACGGGTGTG GAGCCCCAGG AGTGCACCAG CTTCAGTGAG
    CAGCTGCAGC AGATCCCAGC TGGGGTGACT GTGTGCGTGC TGACCCTGGT GAGCATCCAG
    CCCACCTCCG TGGGGGACAC ACTGCTGCTG ACACGGTTGG AGAGGGGCAG CGCTCCCATC
    ACTGTCCGCA TCCCAACTGC GCACACCAAG ACCCCGCTGA GCTCAGTGCT GAGTGACTTC
    GACTCCATCC AGAAGGAGCA GAAGGATGCC AACGGCTACA CTGAAAAGAG AGCCTGGTGG
    CTGTGCCGCT TCGAGCTGGA CCAGAGGATG AAGAACCTCA TTGAGACGCT GGAAATGCAG
    GTGCTGGGTT GCTGGAAGGC AGCGCTGCTC CCTGCAGCCC AGGATCCCCT GCTGGCTGAG
    GAAGCTGCCC GTTTATGTGC TCAGCTGCAG CAGTGTGGCT GGTGGGGCAA AGATCCGACC
    CTACTCAAGG TGCTGCTGAA CGCCGCTGCC CTCCTCAGCC CTGCTGATGT GCAAGCCCTG
    GCCGTGGGGC TGTGCCCAGC TCAGCCCAGC AGAGCCCGGC TCCTCTTGCA GGAGGCTCTG
    GACAAGCAGA AAAACAGCAC ACGGGAGCCG GGCGGTTCCG TCATCCTGGT GCTGGATAAG
    CACCTGCAGA AGCTGCCCTG GGAGAGCATG GCGTGCCTGA GGGACCTGCC CGTCACCCGG
    CTCCCATCCC TGCGCTTCCT CCTCAGCTAC ACCCTGACAC AGAGGCAGAC AAACTCCGTG
    CTGTGCCGCG GCATTGACCC CAGCAGGACC TTCTATGTCC TCAACCCTCA GAACAACCTC
    TCGAGCACTG AGGAGTGTTT CCGCAGCTGG TTTGAGAGTG AACCAGGCTG GAGAGGGGTG
    ACAGGATCAG TCCCCAGCCA GGAGCAGATG CAGGCAGCGC TGCTGGAGCA CGATCTCTAC
    ATCTACGCGG GACACGGAGC CGGTGCCCAC CTGCTGGACG GGCAGACCAT CTCCCAGCTG
    CAGTGCCGTG CTGTCGCTCT GCTCTTCGGC TGCAGCAGCG CCGCCCTCGC CGTGCGTGGC
    AGCCTGGAGG GTACGGGCAT TGTGCTGAAG TACATCATGG CTGGCTGCCC CCTCGTCTTG
    GGCAACCTGT GGGACGTCAC CGACCGCGAC ATTGACCGCT ATGCTGAGGC ACTGCTGCGT
    GCCTGGCTGG GGGCTCCTCG GCCCGGAGCC CCCCTTCTCC ACTACATGGC CGAGGCCCGG
    CGTGCCCCCC GCCTCCAGCA CCTCATCGGG GCCGCCCCCG TGGCCTATGG GCTCCCTGTG
    GCCCTGCAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015128533.1
    CDS566..7297
    Translation

    Target ORF information:

    RefSeq Version XM_015273047.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015273047.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGAATCGCC TCAAAGGCAC CGACTTTGTG TCCCGCACTG CCAGCCCGGA GGGCACAGAG 
    GAGCTGCTCA GGGAGCTGTT GCAATGCCTC TCCAGCAGCA CGGTGGTGCC GGGCAGTGTG
    CAGAGCACGG TGTGCGACCG CGTCCTGCGG GCCTGCGTGC AGCGCCTGGC GGAGCCCGGC
    TGCGGCCTCA CGCACACCGA CGCGGTGCTG GCGTTGGCCG AGGCGGCCTG CAGGGGCTTC
    GTCACCGCCG CCACCGCCCC GCCGAGCCCT CTCTACCTGG AGAAGATCCT CTACTACCTG
    CTGAAGAACG CGGCTGCCCG CGGCCATCGC AGTGCCTGCA GGCGGGCGGC GGAGCTCCTC
    CACGCTCAGC TCTGCGCCCA CCAGCAGAGC CACGCGCCGT CCGCCGACTT CGGTGCTGTT
    GTCCATAGCA GCTTCTGCAC GCTGTGGGGC GCCGCGGGGT CGCTGGGGGA CCGCCTGCAG
    GCCCTGCGCT TCCTGCTGCT GCTGGAGGAC GAGGAGGGGG CCGTGCTGCC CCTGGAGCCG
    CCCTTCTTCA CCTCGCAGAC CGCCCGGCAG GCGGTGTTGG CCGCCACTCT CTCCAAAGCC
    CACCACGCTC CATCCTCGGC TTGCTTTGGC CGGGAGCTGA GGGGTATCCT GACCTCCCTG
    CGCGAGGGCG CGGCGCGGCC CCCCTCCCTG CCCCACGCCC TCTGCTTCTT GGAGCTGGCG
    TTGGAGCAGT GCCGGCAGCT CTGCCAGAGC GGCTGCCACG GGGAGGCGGA GGCGGCGGTG
    AGGGATGCGC GAGGATTCGT GGGGGGCTCT GAGGCGTTCG GCGAGGCCCT GGCCCTGCTG
    GAGGCCGGCG TGCAGCTGAG CCGGGCGCTG GCCCCGAACG GGGGCTCAGC AGGGCCGGCC
    CTGGCGCAGG CGGCGGCAGC GCTGGGCGGC GCAGCGAAGG CCTCGGGGCG CTTCCTCAGG
    GTGCTGGCCG AGGGCTGCCA GTTCGTCACC GCGTCCCTCC ACCAGCGTGC CAGGAGCAGC
    CAGCAGCAGC CGATGCCTCG GGACGACGTG CTCCAGCTCT GCGCCTTTGT GCGGGGGCAC
    TGCCGTGTCC TCGGCCTGCT GCTGCGCGTG GCGCCTCCTG ACGGCGTGAA GCAGCGGCTC
    GTGCTGAAGC AGCTGCTGTA CCACAGCCTC CAGCTCTTCA CCAGCGTGGC CTGTGACACC
    TTCCGCTCCT CCCAGGTGGC CGGGTGGTCA GGGCAGGAAC GGCTGACATT GGGCTGCCAG
    GACACCGTGA TGCAGATGCT GGAGGCTCTG GAGGGACTTC CCCAGAGGGA GCAAGCCAAG
    TACCTCGACA TTACCGCATC GTGCACGTTT AAGCTGGCAT ACATCTTCTA CAGCCAGAAC
    CTTTACAAGG AAGCCAGCTC CGTCTCCGAG CTCTTCTGCA AGAGGCTGAA GCAAATAGAT
    GCCTTCAGAT TTCCAGAGAT ACCTCCCGAA AAGCTGCACA GGTGCTTCAG GCTGCAGGTG
    GAAAGCTACC AAAAGCTGGG CCTGCTGGAG AGCGCGCTGG CGTCCTGCGT GCAGTGGCTG
    ACGTCGCTGC GGGGCCGCGT TGGGGAGCTG CTGGCGGAGC CCATCGGCCT CTGGGCCCGA
    GTGAAAATGG ACAGCGTGAA GCAGGGAGCG GAGGACTTAC GGCTGAGAAC CCTGAAGGAA
    GAACTGTCTG CTCACCACCT GGACGCGGAC ACCTTGGTGG CTGTGCTCTT TGCCGAGCTG
    AAGGCTTACA AGAGCATCCG TGCCGACACA GGGCAGGAAC GCTACAACGT GCTCTGCGAC
    CTGCTGGAGA TCTGCTCCGA GGACAGCGGC CGCCTGCATG AGCGCGCTGC CTGCTTGGTG
    GAGCTGGCCC AGGTCCTTTG CTACCACAAC TATGCCCAGC AAACCGACTG CTCCGCGTTG
    GATTCTGTCC ACGAAGCACT GCGGCTGTTG GATTCGGTGC CCCGGAGTGC TGAGAACCGG
    GAGCAGCTCC TCGATGACCG GGCGCAGGCT CTGCTCTGGC TCTACATCTG CACCCTGGAG
    TCCAAGCTGG AGGAGGGCAT AGCAAGGGAG CAGAGAGTCA AAGCTCTGGG ACAGAAGAGC
    CTGGAAGACT TTGAGCCCAA TGACCTCAAC TATGAAAGCA GGCTGCAGGA GGATGCATTC
    CTGAACAGCG GCATCTCCTT CAACCTGCTG ACAGAATTGG TGGTGGTGCT GGTGGTGGTT
    CAGTGCTGTG GCGAAGCTGC TCAGCCCCGT GCTGTGCCCA CAGCTCAGTC CAAGAGCCTG
    GACGATGCCT TAGCCCTATG GAAGCAGCTG CTGGCATCTC AGGGCATCCC AGCTGTGCGC
    AGTGTGGAGC AGACCACGGC CTCTCTTCGT GTTACAGCAG CTCTCTACAG GCTGATGGAC
    AAGCCTCTGC AAGCCATGGA AAGCTACCTG CTGGTGCGAG CCCTGTGCAG CGCACTTGGG
    GACAATTTGG GCACGGCCGG AGCTCTCTGC CAGGTCACCA AACTGCTCCT CCAGCTGGAA
    TGCCCCAGCT ACGCCAAGCT CTTCCTGGAA GAGGCAGAGT CCTGCTTGCA GAGAACAGAC
    GGGGGCAGCG ACTCCTACCT GCTGCTGCAG CAGACCTGCC TCGTGCTCCG CAGCCAGCTG
    TACTGCGCCA GCCACCGCAT TAAAGATGGC ACTGCCCTCT TGCTGGAGGT GCTCCAGAAC
    CCGGCTCTGC AGAAGACTGC CAAGGTCTGG TACATGCTCC AAGCACAAGT CCTGCAACTG
    ACCGCCACCT ACCTGAGCCT GCCGCCCAGC CACCTCTCGC CAGAGTTCAG GCAGTACATC
    TTCACGCAGG GGTGGAAGAC TCCCGAGACA GCTCTGAGCG AAGCCCACAG GTTGTTACGT
    GGCATCGTCC TCCTCCTGAT GGGCAGCAAC GTGCTGGGCT CCCACAAGTC AGCAGCAGAT
    ATCCGGTTCA TAGATTGTGG GGACAACGTA CTGCAGAAGT GGCAAGTGCT GGCCGACTTG
    CTGGGCTGCT CAGAGCACCT GGTGGTCCTC CTCAGCAGGG TGGAGGTGAT GTGCAAAGCC
    AAAGCCTTCT GCTTGGAGGC CATCAAGCTG GCTGTGAAAC TGCAGGCGGT CCGATGGTGT
    GCATCCTTCC TGGTGCTGAA GGCTCAGCTG GAGCTGCAGC AGAGTGAGCT GGAGCTGAGC
    CACTGGAACC TGCAGCAGGC TCTGTTCCTC CTGGAGTCCG GCACCGAGTT TGAGGCTGAT
    GAGAAGCAGC GAGCCCCCCT GAAGATCTTG CCCAGGAAAG GCAAACCAGA GGGCAGGAAG
    CAGCGGGATG AGGGCTCTGA GCTCCCGGGA GAAGATGAGG GCTTTCTGAA GGGCCCTGCG
    CTGGAGTTTG TGGCCATGGT GAGCGGCCTG GAGAAAGCAG ATGACCTCAG CTCCTCGCCA
    CAGCTGAAAC CCAAGAGGAA GAGGAGGCTC GCCTTCCTCG CCCACCCCGC TGCCTGCCCC
    TGCTGCCTCT GCTCCGACCT GACCCTGTCG TCCCTCTGCC TCCGCTGGCT GCTCTGCTGT
    GCGCGGGGTG AGCTGGCGGC CGGCAGTGCA GCCGAAGGGC TGGGGCTGAT CCAAGCCACC
    CTGCAGCGCT GCGGCACCGT GGGCCCTCGC TTCACCGTCC TGCTGCAGGA CAAGCTGGGC
    AGTGGTCACA AGGCACCTGC ACTGCGGCTG TTAGATGATT TGGTGGCCAC TGGCTATGCC
    GCACTGGCCC TTCACAGCCT GGCTGGTCTC TGTCCTGAGG AGAAGCTGGA GGAGCACCTG
    AAAGCCGGTC TGTCTTTCCT GGCATCCTGC AGCCTCCACC TGCCCAGCCT GGGGGTCTCC
    AAGGCCAGCC TGCTGCTTGC CAAAGCCACG GCTGCTGTTT GCCGCTTGGC CGCCAAGCAC
    GGAGGCTCTG TGGACGCTGT CTTTGCCTCT GCTTGGGCTT GGCAGCCAGC CCCACTGAGC
    CCCACCGAGC CCAAAGCAGC AGCGGCTGCG CCCAGGATGG ACAAAGCTCA ACTAAAGAGG
    CTTAAAAACA AAGCGGCAAC CACAGCAGCC CCTGTGCTCA AACCCAAAGC CAAGGAGACC
    CAGAGAGCAA AGCCCCCCTC GGTGGGAGAT ACCAACGATG CCTTTCTCGT GACCGATTCC
    GACACAGAGC CGCCCGCTGC TGTTGGTCAG ACTGCGACAA AGTCCTGCAC CACACGCCCC
    AATGCCCGTC CCTGCAGCAA AGCACGTGCA CAGGGCTCTG TGGCATCCCC TGCACGGAAA
    CCCAGAGGGA AGAAGAAGCA GCAAGCCAAG AGCACAGACA CCGACGATGC CTTCGCTTTG
    GGTGACTCGG ACAGCAAGGT GCCTCCCATT GTGGTCCGGC CGGCAGCAGT GCCCTGCACT
    CCGCGCCAGA AGCCGTCCTG TCCTCTAGCC AAGACATACA CCCGGGGGGC TGCGATGTCC
    CTACGGCCCA GGGCTCCCTT CACCGTGTTC AGCGAGGCCT CGCCACCACT CAGCAAGTCA
    CAGCTCCTGA AAGCACCCAA AGCCTGTGGG AAAACCAGAT CCCGGCTGCC GGTGACATTC
    AGTGATGACA GCGACACCGA GGGTCTCAAA TCAGTGCTGA GCCCCATTGC TGGCAAAGCA
    CCCATTGCCG GCTGCAAGAC GCCGAAGTCA GTGTGCTGCT CAAAGAGAGC TCTGCTCCCC
    CGGTCCTTGG AGATGGGGGG CTCCCAGCTG AGCTCCTCCT CCGTGGGCAG AGGCTGTAGG
    AGCAGCACCC GGCCCCGGCG GGGGCGCACA GCTGGGGGCA CCGAGAAGAA GGAGCGGGCA
    GCACGCAGGG CTCCGAGCTG GAAAGGGGAT GAGGAACGGG AGCTGCTGTG TGCCATCGAA
    GAGAAGGAAA AGGTGGAGGA GCAGGTCAAC GTCAGCTTGG AGCTACTCCA AGCCTCTGAG
    GATGAAGCCC AGGGTGGAAT CCAACGCAGG CTGGAGGGCG CGGACATGGA GCTCGAGGGG
    CTGCGGCAGG AGGTTGGTGG AGAGGTCCTG GCACGGCAGA GGCTGGGCAG TACGGAGCCC
    CTGTGCCTCA GCGCCCTGCA CAACGTGCTG CCAGCTGTGG ATGACGCCTC CTTGCTGGAC
    ACTGCCTGCG GGCTCCTGAA ATCTGCCTTC AGCTGCATCA GCCACTGCCC ACCCGGCACG
    CTGTACAGCC AGCTCTGTCA GCTCCTGGCT CTGGCCACGG GTGATCAGGA CCCCATCACC
    ACTGCCTACC TGCTGGCTGA GTCTGTCTCG GTCACCACGC GCCATCAGCT GCTCAGTATC
    ATCCACAAGA AGCTTCATAA GGAGAAGAAA GCAGCAGCAG ATGTGGCTGA GCAGCTCCAA
    GGGCTGACCC TGCAGGATAA GGATGCTCCT TCACACCACT GCCACCTCAC TGAGCTCCAG
    GACCTCTTCA GCTTCAGCCC CACGGGTGTG GAGCCCCAGG AGTGCACCAG CTTCAGTGAG
    CAGCTGCAGC AGATCCCAGC TGGGGTGACT GTGTGCGTGC TGACCCTGGT GAGCATCCAG
    CCCACCTCCG TGGGGGACAC ACTGCTGCTG ACACGGTTGG AGAGGGGCAG CGCTCCCATC
    ACTGTCCGCA TCCCAACTGC GCACACCAAG ACCCCGCTGA GCTCAGTGCT GAGTGACTTC
    GACTCCATCC AGAAGGAGCA GAAGGATGCC AACGGCTACA CTGAAAAGAG AGCCTGGTGG
    CTGTGCCGCT TCGAGCTGGA CCAGAGGATG AAGAACCTCA TTGAGACGCT GGAAATGCAG
    GTGCTGGGTT GCTGGAAGGC AGCGCTGCTC CCTGCAGCCC AGGATCCCCT GCTGGCTGAG
    GAAGCTGCCC GTTTATGTGC TCAGCTGCAG CAGTGTGGCT GGTGGGGCAA AGATCCGACC
    CTACTCAAGG TGCTGCTGAA CGCCGCTGCC CTCCTCAGCC CTGCTGATGT GCAAGCCCTG
    GCCGTGGGGC TGTGCCCAGC TCAGCCCAGC AGAGCCCGGC TCCTCTTGCA GGAGGCTCTG
    GACAAGCAGA AAAACAGCAC ACGGGAGCCG GGCGGTTCCG TCATCCTGGT GCTGGATAAG
    CACCTGCAGA AGCTGCCCTG GGAGAGCATG GCGTGCCTGA GGGACCTGCC CGTCACCCGG
    CTCCCATCCC TGCGCTTCCT CCTCAGCTAC ACCCTGACAC AGAGGCAGAC AAACTCCGTG
    CTGTGCCGCG GCATTGACCC CAGCAGGACC TTCTATGTCC TCAACCCTCA GAACAACCTC
    TCGAGCACTG AGGAGTGTTT CCGCAGCTGG TTTGAGAGTG AACCAGGCTG GAGAGGGGTG
    ACAGGATCAG TCCCCAGCCA GGAGCAGATG CAGGCAGCGC TGCTGGAGCA CGATCTCTAC
    ATCTACGCGG GACACGGAGC CGGTGCCCAC CTGCTGGACG GGCAGACCAT CTCCCAGCTG
    CAGTGCCGTG CTGTCGCTCT GCTCTTCGGC TGCAGCAGCG CCGCCCTCGC CGTGCGTGGC
    AGCCTGGAGG GTACGGGCAT TGTGCTGAAG TACATCATGG CTGGCTGCCC CCTCGTCTTG
    GGCAACCTGT GGGACGTCAC CGACCGCGAC ATTGACCGCT ATGCTGAGGC ACTGCTGCGT
    GCCTGGCTGG GGGCTCCTCG GCCCGGAGCC CCCCTTCTCC ACTACATGGC CGAGGCCCGG
    CGTGCCCCCC GCCTCCAGCA CCTCATCGGG GCCGCCCCCG TGGCCTATGG GCTCCCTGTG
    GCCCTGCAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa50165
    Clone ID Related Accession (Same CDS sequence) XM_015273048.1 , XM_025145487.1
    Accession Version XM_015273048.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6669bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product separin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_464144.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGAATCGCC TCAAAGGCAC CGACTTTGTG TCCCGCACTG CCAGCCCGGA GGGCACAGAG 
    GAGCTGCTCA GGGAGCTGTT GCAATGCCTC TCCAGCAGCA CGGTGGTGCC GGGCAGTGTG
    CAGAGCACGG TGTGCGACCG CGTCCTGCGG GCCTGCGTGC AGCGCCTGGC GGAGCCCGGC
    TGCGGCCTCA CGCACACCGA CGCGGTGCTG GCGTTGGCCG AGGCGGCCTG CAGGGGCTTC
    GTCACCGCCG CCACCGCCCC GCCGAGCCCT CTCTACCTGG AGAAGATCCT CTACTACCTG
    CTGAAGAACG CGGCTGCCCG CGGCCATCGC AGTGCCTGCA GGCGGGCGGC GGAGCTCCTC
    CACGCTCAGC TCTGCGCCCA CCAGCAGAGC CACGCGCCGT CCGCCGACTT CGGTGCTGTT
    GTCCATAGCA GCTTCTGCAC GCTGTGGGGC GCCGCGGGGT CGCTGGGGGA CCGCCTGCAG
    GCCCTGCGCT TCCTGCTGCT GCTGGAGGAC GAGGAGGGGG CCGTGCTGCC CCTGGAGCCG
    CCCTTCTTCA CCTCGCAGAC CGCCCGGCAG GCGGTGTTGG CCGCCACTCT CTCCAAAGCC
    CACCACGCTC CATCCTCGGC TTGCTTTGGC CGGGAGCTGA GGGGTATCCT GACCTCCCTG
    CGCGAGGGCG CGGCGCGGCC CCCCTCCCTG CCCCACGCCC TCTGCTTCTT GGAGCTGGCG
    TTGGAGCAGT GCCGGCAGCT CTGCCAGAGC GGCTGCCACG GGGAGGCGGA GGCGGCGGTG
    AGGGATGCGC GAGGATTCGT GGGGGGCTCT GAGGCGTTCG GCGAGGCCCT GGCCCTGCTG
    GAGGCCGGCG TGCAGCTGAG CCGGGCGCTG GCCCCGAACG GGGGCTCAGC AGGGCCGGCC
    CTGGCGCAGG CGGCGGCAGC GCTGGGCGGC GCAGCGAAGG CCTCGGGGCG CTTCCTCAGG
    GTGCTGGCCG AGGGCTGCCA GTTCGTCACC GCGTCCCTCC ACCAGCGTGC CAGGAGCAGC
    CAGCAGCAGC CGATGCCTCG GGACGACGTG CTCCAGCTCT GCGCCTTTGT GCGGGGGCAC
    TGCCGTGTCC TCGGCCTGCT GCTGCGCGTG GCGCCTCCTG ACGGCGTGAA GCAGCGGCTC
    GTGCTGAAGC AGCTGCTGTA CCACAGCCTC CAGCTCTTCA CCAGCGTGGC CTGTGACACC
    TTCCGCTCCT CCCAGGTGGC CGGGTGGTCA GGGCAGGAAC GGCTGACATT GGGCTGCCAG
    GACACCGTGA TGCAGATGCT GGAGGCTCTG GAGGGACTTC CCCAGAGGGA GCAAGCCAAG
    TACCTCGACA TTACCGCATC GTGCACGTTT AAGCTGGCAT ACATCTTCTA CAGCCAGAAC
    CTTTACAAGG AAGCCAGCTC CGTCTCCGAG CTCTTCTGCA AGAGGCTGAA GCAAATAGAT
    GCCTTCAGAT TTCCAGAGAT ACCTCCCGAA AAGCTGCACA GGTGCTTCAG GCTGCAGGTG
    GAAAGCTACC AAAAGCTGGG CCTGCTGGAG AGCGCGCTGG CGTCCTGCGT GCAGTGGCTG
    ACGTCGCTGC GGGGCCGCGT TGGGGAGCTG CTGGCGGAGC CCATCGGCCT CTGGGCCCGA
    GTGAAAATGG ACAGCGTGAA GCAGGGAGCG GAGGACTTAC GGCTGAGAAC CCTGAAGGAA
    GAACTGTCTG CTCACCACCT GGACGCGGAC ACCTTGGTGG CTGTGCTCTT TGCCGAGCTG
    AAGGCTTACA AGAGCATCCG TGCCGACACA GGGCAGGAAC GCTACAACGT GCTCTGCGAC
    CTGCTGGAGA TCTGCTCCGA GGACAGCGGC CGCCTGCATG AGCGCGCTGC CTGCTTGGTG
    GAGCTGGCCC AGGTCCTTTG CTACCACAAC TATGCCCAGC AAACCGACTG CTCCGCGTTG
    GATTCTGTCC ACGAAGCACT GCGGCTGTTG GATTCGGTGC CCCGGAGTGC TGAGAACCGG
    GAGCAGCTCC TCGATGACCG GGCGCAGGCT CTGCTCTGGC TCTACATCTG CACCCTGGAG
    TCCAAGCTGG AGGAGGGCAT AGCAAGGGAG CAGAGAGTCA AAGCTCTGGG ACAGAAGAGC
    CTGGAAGACT TTGAGCCCAA TGACCTCAAC TATGAAAGCA GGCTGCAGGA GGATGCATTC
    CTGAACAGCG GCATCTCCTT CAACCTGCTG ACAGAATTGG CTCAGTCCAA GAGCCTGGAC
    GATGCCTTAG CCCTATGGAA GCAGCTGCTG GCATCTCAGG GCATCCCAGC TGTGCGCAGT
    GTGGAGCAGA CCACGGCCTC TCTTCGTGTT ACAGCAGCTC TCTACAGGCT GATGGACAAG
    CCTCTGCAAG CCATGGAAAG CTACCTGCTG GTGCGAGCCC TGTGCAGCGC ACTTGGGGAC
    AATTTGGGCA CGGCCGGAGC TCTCTGCCAG GTCACCAAAC TGCTCCTCCA GCTGGAATGC
    CCCAGCTACG CCAAGCTCTT CCTGGAAGAG GCAGAGTCCT GCTTGCAGAG AACAGACGGG
    GGCAGCGACT CCTACCTGCT GCTGCAGCAG ACCTGCCTCG TGCTCCGCAG CCAGCTGTAC
    TGCGCCAGCC ACCGCATTAA AGATGGCACT GCCCTCTTGC TGGAGGTGCT CCAGAACCCG
    GCTCTGCAGA AGACTGCCAA GGTCTGGTAC ATGCTCCAAG CACAAGTCCT GCAACTGACC
    GCCACCTACC TGAGCCTGCC GCCCAGCCAC CTCTCGCCAG AGTTCAGGCA GTACATCTTC
    ACGCAGGGGT GGAAGACTCC CGAGACAGCT CTGAGCGAAG CCCACAGGTT GTTACGTGGC
    ATCGTCCTCC TCCTGATGGG CAGCAACGTG CTGGGCTCCC ACAAGTCAGC AGCAGATATC
    CGGTTCATAG ATTGTGGGGA CAACGTACTG CAGAAGTGGC AAGTGCTGGC CGACTTGCTG
    GGCTGCTCAG AGCACCTGGT GGTCCTCCTC AGCAGGGTGG AGGTGATGTG CAAAGCCAAA
    GCCTTCTGCT TGGAGGCCAT CAAGCTGGCT GTGAAACTGC AGGCGGTCCG ATGGTGTGCA
    TCCTTCCTGG TGCTGAAGGC TCAGCTGGAG CTGCAGCAGA GTGAGCTGGA GCTGAGCCAC
    TGGAACCTGC AGCAGGCTCT GTTCCTCCTG GAGTCCGGCA CCGAGTTTGA GGCTGATGAG
    AAGCAGCGAG CCCCCCTGAA GATCTTGCCC AGGAAAGGCA AACCAGAGGG CAGGAAGCAG
    CGGGATGAGG GCTCTGAGCT CCCGGGAGAA GATGAGGGCT TTCTGAAGGG CCCTGCGCTG
    GAGTTTGTGG CCATGGTGAG CGGCCTGGAG AAAGCAGATG ACCTCAGCTC CTCGCCACAG
    CTGAAACCCA AGAGGAAGAG GAGGCTCGCC TTCCTCGCCC ACCCCGCTGC CTGCCCCTGC
    TGCCTCTGCT CCGACCTGAC CCTGTCGTCC CTCTGCCTCC GCTGGCTGCT CTGCTGTGCG
    CGGGGTGAGC TGGCGGCCGG CAGTGCAGCC GAAGGGCTGG GGCTGATCCA AGCCACCCTG
    CAGCGCTGCG GCACCGTGGG CCCTCGCTTC ACCGTCCTGC TGCAGGACAA GCTGGGCAGT
    GGTCACAAGG CACCTGCACT GCGGCTGTTA GATGATTTGG TGGCCACTGG CTATGCCGCA
    CTGGCCCTTC ACAGCCTGGC TGGTCTCTGT CCTGAGGAGA AGCTGGAGGA GCACCTGAAA
    GCCGGTCTGT CTTTCCTGGC ATCCTGCAGC CTCCACCTGC CCAGCCTGGG GGTCTCCAAG
    GCCAGCCTGC TGCTTGCCAA AGCCACGGCT GCTGTTTGCC GCTTGGCCGC CAAGCACGGA
    GGCTCTGTGG ACGCTGTCTT TGCCTCTGCT TGGGCTTGGC AGCCAGCCCC ACTGAGCCCC
    ACCGAGCCCA AAGCAGCAGC GGCTGCGCCC AGGATGGACA AAGCTCAACT AAAGAGGCTT
    AAAAACAAAG CGGCAACCAC AGCAGCCCCT GTGCTCAAAC CCAAAGCCAA GGAGACCCAG
    AGAGCAAAGC CCCCCTCGGT GGGAGATACC AACGATGCCT TTCTCGTGAC CGATTCCGAC
    ACAGAGCCGC CCGCTGCTGT TGGTCAGACT GCGACAAAGT CCTGCACCAC ACGCCCCAAT
    GCCCGTCCCT GCAGCAAAGC ACGTGCACAG GGCTCTGTGG CATCCCCTGC ACGGAAACCC
    AGAGGGAAGA AGAAGCAGCA AGCCAAGAGC ACAGACACCG ACGATGCCTT CGCTTTGGGT
    GACTCGGACA GCAAGGTGCC TCCCATTGTG GTCCGGCCGG CAGCAGTGCC CTGCACTCCG
    CGCCAGAAGC CGTCCTGTCC TCTAGCCAAG ACATACACCC GGGGGGCTGC GATGTCCCTA
    CGGCCCAGGG CTCCCTTCAC CGTGTTCAGC GAGGCCTCGC CACCACTCAG CAAGTCACAG
    CTCCTGAAAG CACCCAAAGC CTGTGGGAAA ACCAGATCCC GGCTGCCGGT GACATTCAGT
    GATGACAGCG ACACCGAGGG TCTCAAATCA GTGCTGAGCC CCATTGCTGG CAAAGCACCC
    ATTGCCGGCT GCAAGACGCC GAAGTCAGTG TGCTGCTCAA AGAGAGCTCT GCTCCCCCGG
    TCCTTGGAGA TGGGGGGCTC CCAGCTGAGC TCCTCCTCCG TGGGCAGAGG CTGTAGGAGC
    AGCACCCGGC CCCGGCGGGG GCGCACAGCT GGGGGCACCG AGAAGAAGGA GCGGGCAGCA
    CGCAGGGCTC CGAGCTGGAA AGGGGATGAG GAACGGGAGC TGCTGTGTGC CATCGAAGAG
    AAGGAAAAGG TGGAGGAGCA GGTCAACGTC AGCTTGGAGC TACTCCAAGC CTCTGAGGAT
    GAAGCCCAGG GTGGAATCCA ACGCAGGCTG GAGGGCGCGG ACATGGAGCT CGAGGGGCTG
    CGGCAGGAGG TTGGTGGAGA GGTCCTGGCA CGGCAGAGGC TGGGCAGTAC GGAGCCCCTG
    TGCCTCAGCG CCCTGCACAA CGTGCTGCCA GCTGTGGATG ACGCCTCCTT GCTGGACACT
    GCCTGCGGGC TCCTGAAATC TGCCTTCAGC TGCATCAGCC ACTGCCCACC CGGCACGCTG
    TACAGCCAGC TCTGTCAGCT CCTGGCTCTG GCCACGGGTG ATCAGGACCC CATCACCACT
    GCCTACCTGC TGGCTGAGTC TGTCTCGGTC ACCACGCGCC ATCAGCTGCT CAGTATCATC
    CACAAGAAGC TTCATAAGGA GAAGAAAGCA GCAGCAGATG TGGCTGAGCA GCTCCAAGGG
    CTGACCCTGC AGGATAAGGA TGCTCCTTCA CACCACTGCC ACCTCACTGA GCTCCAGGAC
    CTCTTCAGCT TCAGCCCCAC GGGTGTGGAG CCCCAGGAGT GCACCAGCTT CAGTGAGCAG
    CTGCAGCAGA TCCCAGCTGG GGTGACTGTG TGCGTGCTGA CCCTGGTGAG CATCCAGCCC
    ACCTCCGTGG GGGACACACT GCTGCTGACA CGGTTGGAGA GGGGCAGCGC TCCCATCACT
    GTCCGCATCC CAACTGCGCA CACCAAGACC CCGCTGAGCT CAGTGCTGAG TGACTTCGAC
    TCCATCCAGA AGGAGCAGAA GGATGCCAAC GGCTACACTG AAAAGAGAGC CTGGTGGCTG
    TGCCGCTTCG AGCTGGACCA GAGGATGAAG AACCTCATTG AGACGCTGGA AATGCAGGTG
    CTGGGTTGCT GGAAGGCAGC GCTGCTCCCT GCAGCCCAGG ATCCCCTGCT GGCTGAGGAA
    GCTGCCCGTT TATGTGCTCA GCTGCAGCAG TGTGGCTGGT GGGGCAAAGA TCCGACCCTA
    CTCAAGGTGC TGCTGAACGC CGCTGCCCTC CTCAGCCCTG CTGATGTGCA AGCCCTGGCC
    GTGGGGCTGT GCCCAGCTCA GCCCAGCAGA GCCCGGCTCC TCTTGCAGGA GGCTCTGGAC
    AAGCAGAAAA ACAGCACACG GGAGCCGGGC GGTTCCGTCA TCCTGGTGCT GGATAAGCAC
    CTGCAGAAGC TGCCCTGGGA GAGCATGGCG TGCCTGAGGG ACCTGCCCGT CACCCGGCTC
    CCATCCCTGC GCTTCCTCCT CAGCTACACC CTGACACAGA GGCAGACAAA CTCCGTGCTG
    TGCCGCGGCA TTGACCCCAG CAGGACCTTC TATGTCCTCA ACCCTCAGAA CAACCTCTCG
    AGCACTGAGG AGTGTTTCCG CAGCTGGTTT GAGAGTGAAC CAGGCTGGAG AGGGGTGACA
    GGATCAGTCC CCAGCCAGGA GCAGATGCAG GCAGCGCTGC TGGAGCACGA TCTCTACATC
    TACGCGGGAC ACGGAGCCGG TGCCCACCTG CTGGACGGGC AGACCATCTC CCAGCTGCAG
    TGCCGTGCTG TCGCTCTGCT CTTCGGCTGC AGCAGCGCCG CCCTCGCCGT GCGTGGCAGC
    CTGGAGGGTA CGGGCATTGT GCTGAAGTAC ATCATGGCTG GCTGCCCCCT CGTCTTGGGC
    AACCTGTGGG ACGTCACCGA CCGCGACATT GACCGCTATG CTGAGGCACT GCTGCGTGCC
    TGGCTGGGGG CTCCTCGGCC CGGAGCCCCC CTTCTCCACT ACATGGCCGA GGCCCGGCGT
    GCCCCCCGCC TCCAGCACCT CATCGGGGCC GCCCCCGTGG CCTATGGGCT CCCTGTGGCC
    CTGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015128534.1
    CDS566..7234
    Translation

    Target ORF information:

    RefSeq Version XM_015273048.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015273048.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGAATCGCC TCAAAGGCAC CGACTTTGTG TCCCGCACTG CCAGCCCGGA GGGCACAGAG 
    GAGCTGCTCA GGGAGCTGTT GCAATGCCTC TCCAGCAGCA CGGTGGTGCC GGGCAGTGTG
    CAGAGCACGG TGTGCGACCG CGTCCTGCGG GCCTGCGTGC AGCGCCTGGC GGAGCCCGGC
    TGCGGCCTCA CGCACACCGA CGCGGTGCTG GCGTTGGCCG AGGCGGCCTG CAGGGGCTTC
    GTCACCGCCG CCACCGCCCC GCCGAGCCCT CTCTACCTGG AGAAGATCCT CTACTACCTG
    CTGAAGAACG CGGCTGCCCG CGGCCATCGC AGTGCCTGCA GGCGGGCGGC GGAGCTCCTC
    CACGCTCAGC TCTGCGCCCA CCAGCAGAGC CACGCGCCGT CCGCCGACTT CGGTGCTGTT
    GTCCATAGCA GCTTCTGCAC GCTGTGGGGC GCCGCGGGGT CGCTGGGGGA CCGCCTGCAG
    GCCCTGCGCT TCCTGCTGCT GCTGGAGGAC GAGGAGGGGG CCGTGCTGCC CCTGGAGCCG
    CCCTTCTTCA CCTCGCAGAC CGCCCGGCAG GCGGTGTTGG CCGCCACTCT CTCCAAAGCC
    CACCACGCTC CATCCTCGGC TTGCTTTGGC CGGGAGCTGA GGGGTATCCT GACCTCCCTG
    CGCGAGGGCG CGGCGCGGCC CCCCTCCCTG CCCCACGCCC TCTGCTTCTT GGAGCTGGCG
    TTGGAGCAGT GCCGGCAGCT CTGCCAGAGC GGCTGCCACG GGGAGGCGGA GGCGGCGGTG
    AGGGATGCGC GAGGATTCGT GGGGGGCTCT GAGGCGTTCG GCGAGGCCCT GGCCCTGCTG
    GAGGCCGGCG TGCAGCTGAG CCGGGCGCTG GCCCCGAACG GGGGCTCAGC AGGGCCGGCC
    CTGGCGCAGG CGGCGGCAGC GCTGGGCGGC GCAGCGAAGG CCTCGGGGCG CTTCCTCAGG
    GTGCTGGCCG AGGGCTGCCA GTTCGTCACC GCGTCCCTCC ACCAGCGTGC CAGGAGCAGC
    CAGCAGCAGC CGATGCCTCG GGACGACGTG CTCCAGCTCT GCGCCTTTGT GCGGGGGCAC
    TGCCGTGTCC TCGGCCTGCT GCTGCGCGTG GCGCCTCCTG ACGGCGTGAA GCAGCGGCTC
    GTGCTGAAGC AGCTGCTGTA CCACAGCCTC CAGCTCTTCA CCAGCGTGGC CTGTGACACC
    TTCCGCTCCT CCCAGGTGGC CGGGTGGTCA GGGCAGGAAC GGCTGACATT GGGCTGCCAG
    GACACCGTGA TGCAGATGCT GGAGGCTCTG GAGGGACTTC CCCAGAGGGA GCAAGCCAAG
    TACCTCGACA TTACCGCATC GTGCACGTTT AAGCTGGCAT ACATCTTCTA CAGCCAGAAC
    CTTTACAAGG AAGCCAGCTC CGTCTCCGAG CTCTTCTGCA AGAGGCTGAA GCAAATAGAT
    GCCTTCAGAT TTCCAGAGAT ACCTCCCGAA AAGCTGCACA GGTGCTTCAG GCTGCAGGTG
    GAAAGCTACC AAAAGCTGGG CCTGCTGGAG AGCGCGCTGG CGTCCTGCGT GCAGTGGCTG
    ACGTCGCTGC GGGGCCGCGT TGGGGAGCTG CTGGCGGAGC CCATCGGCCT CTGGGCCCGA
    GTGAAAATGG ACAGCGTGAA GCAGGGAGCG GAGGACTTAC GGCTGAGAAC CCTGAAGGAA
    GAACTGTCTG CTCACCACCT GGACGCGGAC ACCTTGGTGG CTGTGCTCTT TGCCGAGCTG
    AAGGCTTACA AGAGCATCCG TGCCGACACA GGGCAGGAAC GCTACAACGT GCTCTGCGAC
    CTGCTGGAGA TCTGCTCCGA GGACAGCGGC CGCCTGCATG AGCGCGCTGC CTGCTTGGTG
    GAGCTGGCCC AGGTCCTTTG CTACCACAAC TATGCCCAGC AAACCGACTG CTCCGCGTTG
    GATTCTGTCC ACGAAGCACT GCGGCTGTTG GATTCGGTGC CCCGGAGTGC TGAGAACCGG
    GAGCAGCTCC TCGATGACCG GGCGCAGGCT CTGCTCTGGC TCTACATCTG CACCCTGGAG
    TCCAAGCTGG AGGAGGGCAT AGCAAGGGAG CAGAGAGTCA AAGCTCTGGG ACAGAAGAGC
    CTGGAAGACT TTGAGCCCAA TGACCTCAAC TATGAAAGCA GGCTGCAGGA GGATGCATTC
    CTGAACAGCG GCATCTCCTT CAACCTGCTG ACAGAATTGG CTCAGTCCAA GAGCCTGGAC
    GATGCCTTAG CCCTATGGAA GCAGCTGCTG GCATCTCAGG GCATCCCAGC TGTGCGCAGT
    GTGGAGCAGA CCACGGCCTC TCTTCGTGTT ACAGCAGCTC TCTACAGGCT GATGGACAAG
    CCTCTGCAAG CCATGGAAAG CTACCTGCTG GTGCGAGCCC TGTGCAGCGC ACTTGGGGAC
    AATTTGGGCA CGGCCGGAGC TCTCTGCCAG GTCACCAAAC TGCTCCTCCA GCTGGAATGC
    CCCAGCTACG CCAAGCTCTT CCTGGAAGAG GCAGAGTCCT GCTTGCAGAG AACAGACGGG
    GGCAGCGACT CCTACCTGCT GCTGCAGCAG ACCTGCCTCG TGCTCCGCAG CCAGCTGTAC
    TGCGCCAGCC ACCGCATTAA AGATGGCACT GCCCTCTTGC TGGAGGTGCT CCAGAACCCG
    GCTCTGCAGA AGACTGCCAA GGTCTGGTAC ATGCTCCAAG CACAAGTCCT GCAACTGACC
    GCCACCTACC TGAGCCTGCC GCCCAGCCAC CTCTCGCCAG AGTTCAGGCA GTACATCTTC
    ACGCAGGGGT GGAAGACTCC CGAGACAGCT CTGAGCGAAG CCCACAGGTT GTTACGTGGC
    ATCGTCCTCC TCCTGATGGG CAGCAACGTG CTGGGCTCCC ACAAGTCAGC AGCAGATATC
    CGGTTCATAG ATTGTGGGGA CAACGTACTG CAGAAGTGGC AAGTGCTGGC CGACTTGCTG
    GGCTGCTCAG AGCACCTGGT GGTCCTCCTC AGCAGGGTGG AGGTGATGTG CAAAGCCAAA
    GCCTTCTGCT TGGAGGCCAT CAAGCTGGCT GTGAAACTGC AGGCGGTCCG ATGGTGTGCA
    TCCTTCCTGG TGCTGAAGGC TCAGCTGGAG CTGCAGCAGA GTGAGCTGGA GCTGAGCCAC
    TGGAACCTGC AGCAGGCTCT GTTCCTCCTG GAGTCCGGCA CCGAGTTTGA GGCTGATGAG
    AAGCAGCGAG CCCCCCTGAA GATCTTGCCC AGGAAAGGCA AACCAGAGGG CAGGAAGCAG
    CGGGATGAGG GCTCTGAGCT CCCGGGAGAA GATGAGGGCT TTCTGAAGGG CCCTGCGCTG
    GAGTTTGTGG CCATGGTGAG CGGCCTGGAG AAAGCAGATG ACCTCAGCTC CTCGCCACAG
    CTGAAACCCA AGAGGAAGAG GAGGCTCGCC TTCCTCGCCC ACCCCGCTGC CTGCCCCTGC
    TGCCTCTGCT CCGACCTGAC CCTGTCGTCC CTCTGCCTCC GCTGGCTGCT CTGCTGTGCG
    CGGGGTGAGC TGGCGGCCGG CAGTGCAGCC GAAGGGCTGG GGCTGATCCA AGCCACCCTG
    CAGCGCTGCG GCACCGTGGG CCCTCGCTTC ACCGTCCTGC TGCAGGACAA GCTGGGCAGT
    GGTCACAAGG CACCTGCACT GCGGCTGTTA GATGATTTGG TGGCCACTGG CTATGCCGCA
    CTGGCCCTTC ACAGCCTGGC TGGTCTCTGT CCTGAGGAGA AGCTGGAGGA GCACCTGAAA
    GCCGGTCTGT CTTTCCTGGC ATCCTGCAGC CTCCACCTGC CCAGCCTGGG GGTCTCCAAG
    GCCAGCCTGC TGCTTGCCAA AGCCACGGCT GCTGTTTGCC GCTTGGCCGC CAAGCACGGA
    GGCTCTGTGG ACGCTGTCTT TGCCTCTGCT TGGGCTTGGC AGCCAGCCCC ACTGAGCCCC
    ACCGAGCCCA AAGCAGCAGC GGCTGCGCCC AGGATGGACA AAGCTCAACT AAAGAGGCTT
    AAAAACAAAG CGGCAACCAC AGCAGCCCCT GTGCTCAAAC CCAAAGCCAA GGAGACCCAG
    AGAGCAAAGC CCCCCTCGGT GGGAGATACC AACGATGCCT TTCTCGTGAC CGATTCCGAC
    ACAGAGCCGC CCGCTGCTGT TGGTCAGACT GCGACAAAGT CCTGCACCAC ACGCCCCAAT
    GCCCGTCCCT GCAGCAAAGC ACGTGCACAG GGCTCTGTGG CATCCCCTGC ACGGAAACCC
    AGAGGGAAGA AGAAGCAGCA AGCCAAGAGC ACAGACACCG ACGATGCCTT CGCTTTGGGT
    GACTCGGACA GCAAGGTGCC TCCCATTGTG GTCCGGCCGG CAGCAGTGCC CTGCACTCCG
    CGCCAGAAGC CGTCCTGTCC TCTAGCCAAG ACATACACCC GGGGGGCTGC GATGTCCCTA
    CGGCCCAGGG CTCCCTTCAC CGTGTTCAGC GAGGCCTCGC CACCACTCAG CAAGTCACAG
    CTCCTGAAAG CACCCAAAGC CTGTGGGAAA ACCAGATCCC GGCTGCCGGT GACATTCAGT
    GATGACAGCG ACACCGAGGG TCTCAAATCA GTGCTGAGCC CCATTGCTGG CAAAGCACCC
    ATTGCCGGCT GCAAGACGCC GAAGTCAGTG TGCTGCTCAA AGAGAGCTCT GCTCCCCCGG
    TCCTTGGAGA TGGGGGGCTC CCAGCTGAGC TCCTCCTCCG TGGGCAGAGG CTGTAGGAGC
    AGCACCCGGC CCCGGCGGGG GCGCACAGCT GGGGGCACCG AGAAGAAGGA GCGGGCAGCA
    CGCAGGGCTC CGAGCTGGAA AGGGGATGAG GAACGGGAGC TGCTGTGTGC CATCGAAGAG
    AAGGAAAAGG TGGAGGAGCA GGTCAACGTC AGCTTGGAGC TACTCCAAGC CTCTGAGGAT
    GAAGCCCAGG GTGGAATCCA ACGCAGGCTG GAGGGCGCGG ACATGGAGCT CGAGGGGCTG
    CGGCAGGAGG TTGGTGGAGA GGTCCTGGCA CGGCAGAGGC TGGGCAGTAC GGAGCCCCTG
    TGCCTCAGCG CCCTGCACAA CGTGCTGCCA GCTGTGGATG ACGCCTCCTT GCTGGACACT
    GCCTGCGGGC TCCTGAAATC TGCCTTCAGC TGCATCAGCC ACTGCCCACC CGGCACGCTG
    TACAGCCAGC TCTGTCAGCT CCTGGCTCTG GCCACGGGTG ATCAGGACCC CATCACCACT
    GCCTACCTGC TGGCTGAGTC TGTCTCGGTC ACCACGCGCC ATCAGCTGCT CAGTATCATC
    CACAAGAAGC TTCATAAGGA GAAGAAAGCA GCAGCAGATG TGGCTGAGCA GCTCCAAGGG
    CTGACCCTGC AGGATAAGGA TGCTCCTTCA CACCACTGCC ACCTCACTGA GCTCCAGGAC
    CTCTTCAGCT TCAGCCCCAC GGGTGTGGAG CCCCAGGAGT GCACCAGCTT CAGTGAGCAG
    CTGCAGCAGA TCCCAGCTGG GGTGACTGTG TGCGTGCTGA CCCTGGTGAG CATCCAGCCC
    ACCTCCGTGG GGGACACACT GCTGCTGACA CGGTTGGAGA GGGGCAGCGC TCCCATCACT
    GTCCGCATCC CAACTGCGCA CACCAAGACC CCGCTGAGCT CAGTGCTGAG TGACTTCGAC
    TCCATCCAGA AGGAGCAGAA GGATGCCAAC GGCTACACTG AAAAGAGAGC CTGGTGGCTG
    TGCCGCTTCG AGCTGGACCA GAGGATGAAG AACCTCATTG AGACGCTGGA AATGCAGGTG
    CTGGGTTGCT GGAAGGCAGC GCTGCTCCCT GCAGCCCAGG ATCCCCTGCT GGCTGAGGAA
    GCTGCCCGTT TATGTGCTCA GCTGCAGCAG TGTGGCTGGT GGGGCAAAGA TCCGACCCTA
    CTCAAGGTGC TGCTGAACGC CGCTGCCCTC CTCAGCCCTG CTGATGTGCA AGCCCTGGCC
    GTGGGGCTGT GCCCAGCTCA GCCCAGCAGA GCCCGGCTCC TCTTGCAGGA GGCTCTGGAC
    AAGCAGAAAA ACAGCACACG GGAGCCGGGC GGTTCCGTCA TCCTGGTGCT GGATAAGCAC
    CTGCAGAAGC TGCCCTGGGA GAGCATGGCG TGCCTGAGGG ACCTGCCCGT CACCCGGCTC
    CCATCCCTGC GCTTCCTCCT CAGCTACACC CTGACACAGA GGCAGACAAA CTCCGTGCTG
    TGCCGCGGCA TTGACCCCAG CAGGACCTTC TATGTCCTCA ACCCTCAGAA CAACCTCTCG
    AGCACTGAGG AGTGTTTCCG CAGCTGGTTT GAGAGTGAAC CAGGCTGGAG AGGGGTGACA
    GGATCAGTCC CCAGCCAGGA GCAGATGCAG GCAGCGCTGC TGGAGCACGA TCTCTACATC
    TACGCGGGAC ACGGAGCCGG TGCCCACCTG CTGGACGGGC AGACCATCTC CCAGCTGCAG
    TGCCGTGCTG TCGCTCTGCT CTTCGGCTGC AGCAGCGCCG CCCTCGCCGT GCGTGGCAGC
    CTGGAGGGTA CGGGCATTGT GCTGAAGTAC ATCATGGCTG GCTGCCCCCT CGTCTTGGGC
    AACCTGTGGG ACGTCACCGA CCGCGACATT GACCGCTATG CTGAGGCACT GCTGCGTGCC
    TGGCTGGGGG CTCCTCGGCC CGGAGCCCCC CTTCTCCACT ACATGGCCGA GGCCCGGCGT
    GCCCCCCGCC TCCAGCACCT CATCGGGGCC GCCCCCGTGG CCTATGGGCT CCCTGTGGCC
    CTGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa50165
    Clone ID Related Accession (Same CDS sequence) XM_015273048.1 , XM_025145487.1
    Accession Version XM_025145487.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6669bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product separin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008465.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGAATCGCC TCAAAGGCAC CGACTTTGTG TCCCGCACTG CCAGCCCGGA GGGCACAGAG 
    GAGCTGCTCA GGGAGCTGTT GCAATGCCTC TCCAGCAGCA CGGTGGTGCC GGGCAGTGTG
    CAGAGCACGG TGTGCGACCG CGTCCTGCGG GCCTGCGTGC AGCGCCTGGC GGAGCCCGGC
    TGCGGCCTCA CGCACACCGA CGCGGTGCTG GCGTTGGCCG AGGCGGCCTG CAGGGGCTTC
    GTCACCGCCG CCACCGCCCC GCCGAGCCCT CTCTACCTGG AGAAGATCCT CTACTACCTG
    CTGAAGAACG CGGCTGCCCG CGGCCATCGC AGTGCCTGCA GGCGGGCGGC GGAGCTCCTC
    CACGCTCAGC TCTGCGCCCA CCAGCAGAGC CACGCGCCGT CCGCCGACTT CGGTGCTGTT
    GTCCATAGCA GCTTCTGCAC GCTGTGGGGC GCCGCGGGGT CGCTGGGGGA CCGCCTGCAG
    GCCCTGCGCT TCCTGCTGCT GCTGGAGGAC GAGGAGGGGG CCGTGCTGCC CCTGGAGCCG
    CCCTTCTTCA CCTCGCAGAC CGCCCGGCAG GCGGTGTTGG CCGCCACTCT CTCCAAAGCC
    CACCACGCTC CATCCTCGGC TTGCTTTGGC CGGGAGCTGA GGGGTATCCT GACCTCCCTG
    CGCGAGGGCG CGGCGCGGCC CCCCTCCCTG CCCCACGCCC TCTGCTTCTT GGAGCTGGCG
    TTGGAGCAGT GCCGGCAGCT CTGCCAGAGC GGCTGCCACG GGGAGGCGGA GGCGGCGGTG
    AGGGATGCGC GAGGATTCGT GGGGGGCTCT GAGGCGTTCG GCGAGGCCCT GGCCCTGCTG
    GAGGCCGGCG TGCAGCTGAG CCGGGCGCTG GCCCCGAACG GGGGCTCAGC AGGGCCGGCC
    CTGGCGCAGG CGGCGGCAGC GCTGGGCGGC GCAGCGAAGG CCTCGGGGCG CTTCCTCAGG
    GTGCTGGCCG AGGGCTGCCA GTTCGTCACC GCGTCCCTCC ACCAGCGTGC CAGGAGCAGC
    CAGCAGCAGC CGATGCCTCG GGACGACGTG CTCCAGCTCT GCGCCTTTGT GCGGGGGCAC
    TGCCGTGTCC TCGGCCTGCT GCTGCGCGTG GCGCCTCCTG ACGGCGTGAA GCAGCGGCTC
    GTGCTGAAGC AGCTGCTGTA CCACAGCCTC CAGCTCTTCA CCAGCGTGGC CTGTGACACC
    TTCCGCTCCT CCCAGGTGGC CGGGTGGTCA GGGCAGGAAC GGCTGACATT GGGCTGCCAG
    GACACCGTGA TGCAGATGCT GGAGGCTCTG GAGGGACTTC CCCAGAGGGA GCAAGCCAAG
    TACCTCGACA TTACCGCATC GTGCACGTTT AAGCTGGCAT ACATCTTCTA CAGCCAGAAC
    CTTTACAAGG AAGCCAGCTC CGTCTCCGAG CTCTTCTGCA AGAGGCTGAA GCAAATAGAT
    GCCTTCAGAT TTCCAGAGAT ACCTCCCGAA AAGCTGCACA GGTGCTTCAG GCTGCAGGTG
    GAAAGCTACC AAAAGCTGGG CCTGCTGGAG AGCGCGCTGG CGTCCTGCGT GCAGTGGCTG
    ACGTCGCTGC GGGGCCGCGT TGGGGAGCTG CTGGCGGAGC CCATCGGCCT CTGGGCCCGA
    GTGAAAATGG ACAGCGTGAA GCAGGGAGCG GAGGACTTAC GGCTGAGAAC CCTGAAGGAA
    GAACTGTCTG CTCACCACCT GGACGCGGAC ACCTTGGTGG CTGTGCTCTT TGCCGAGCTG
    AAGGCTTACA AGAGCATCCG TGCCGACACA GGGCAGGAAC GCTACAACGT GCTCTGCGAC
    CTGCTGGAGA TCTGCTCCGA GGACAGCGGC CGCCTGCATG AGCGCGCTGC CTGCTTGGTG
    GAGCTGGCCC AGGTCCTTTG CTACCACAAC TATGCCCAGC AAACCGACTG CTCCGCGTTG
    GATTCTGTCC ACGAAGCACT GCGGCTGTTG GATTCGGTGC CCCGGAGTGC TGAGAACCGG
    GAGCAGCTCC TCGATGACCG GGCGCAGGCT CTGCTCTGGC TCTACATCTG CACCCTGGAG
    TCCAAGCTGG AGGAGGGCAT AGCAAGGGAG CAGAGAGTCA AAGCTCTGGG ACAGAAGAGC
    CTGGAAGACT TTGAGCCCAA TGACCTCAAC TATGAAAGCA GGCTGCAGGA GGATGCATTC
    CTGAACAGCG GCATCTCCTT CAACCTGCTG ACAGAATTGG CTCAGTCCAA GAGCCTGGAC
    GATGCCTTAG CCCTATGGAA GCAGCTGCTG GCATCTCAGG GCATCCCAGC TGTGCGCAGT
    GTGGAGCAGA CCACGGCCTC TCTTCGTGTT ACAGCAGCTC TCTACAGGCT GATGGACAAG
    CCTCTGCAAG CCATGGAAAG CTACCTGCTG GTGCGAGCCC TGTGCAGCGC ACTTGGGGAC
    AATTTGGGCA CGGCCGGAGC TCTCTGCCAG GTCACCAAAC TGCTCCTCCA GCTGGAATGC
    CCCAGCTACG CCAAGCTCTT CCTGGAAGAG GCAGAGTCCT GCTTGCAGAG AACAGACGGG
    GGCAGCGACT CCTACCTGCT GCTGCAGCAG ACCTGCCTCG TGCTCCGCAG CCAGCTGTAC
    TGCGCCAGCC ACCGCATTAA AGATGGCACT GCCCTCTTGC TGGAGGTGCT CCAGAACCCG
    GCTCTGCAGA AGACTGCCAA GGTCTGGTAC ATGCTCCAAG CACAAGTCCT GCAACTGACC
    GCCACCTACC TGAGCCTGCC GCCCAGCCAC CTCTCGCCAG AGTTCAGGCA GTACATCTTC
    ACGCAGGGGT GGAAGACTCC CGAGACAGCT CTGAGCGAAG CCCACAGGTT GTTACGTGGC
    ATCGTCCTCC TCCTGATGGG CAGCAACGTG CTGGGCTCCC ACAAGTCAGC AGCAGATATC
    CGGTTCATAG ATTGTGGGGA CAACGTACTG CAGAAGTGGC AAGTGCTGGC CGACTTGCTG
    GGCTGCTCAG AGCACCTGGT GGTCCTCCTC AGCAGGGTGG AGGTGATGTG CAAAGCCAAA
    GCCTTCTGCT TGGAGGCCAT CAAGCTGGCT GTGAAACTGC AGGCGGTCCG ATGGTGTGCA
    TCCTTCCTGG TGCTGAAGGC TCAGCTGGAG CTGCAGCAGA GTGAGCTGGA GCTGAGCCAC
    TGGAACCTGC AGCAGGCTCT GTTCCTCCTG GAGTCCGGCA CCGAGTTTGA GGCTGATGAG
    AAGCAGCGAG CCCCCCTGAA GATCTTGCCC AGGAAAGGCA AACCAGAGGG CAGGAAGCAG
    CGGGATGAGG GCTCTGAGCT CCCGGGAGAA GATGAGGGCT TTCTGAAGGG CCCTGCGCTG
    GAGTTTGTGG CCATGGTGAG CGGCCTGGAG AAAGCAGATG ACCTCAGCTC CTCGCCACAG
    CTGAAACCCA AGAGGAAGAG GAGGCTCGCC TTCCTCGCCC ACCCCGCTGC CTGCCCCTGC
    TGCCTCTGCT CCGACCTGAC CCTGTCGTCC CTCTGCCTCC GCTGGCTGCT CTGCTGTGCG
    CGGGGTGAGC TGGCGGCCGG CAGTGCAGCC GAAGGGCTGG GGCTGATCCA AGCCACCCTG
    CAGCGCTGCG GCACCGTGGG CCCTCGCTTC ACCGTCCTGC TGCAGGACAA GCTGGGCAGT
    GGTCACAAGG CACCTGCACT GCGGCTGTTA GATGATTTGG TGGCCACTGG CTATGCCGCA
    CTGGCCCTTC ACAGCCTGGC TGGTCTCTGT CCTGAGGAGA AGCTGGAGGA GCACCTGAAA
    GCCGGTCTGT CTTTCCTGGC ATCCTGCAGC CTCCACCTGC CCAGCCTGGG GGTCTCCAAG
    GCCAGCCTGC TGCTTGCCAA AGCCACGGCT GCTGTTTGCC GCTTGGCCGC CAAGCACGGA
    GGCTCTGTGG ACGCTGTCTT TGCCTCTGCT TGGGCTTGGC AGCCAGCCCC ACTGAGCCCC
    ACCGAGCCCA AAGCAGCAGC GGCTGCGCCC AGGATGGACA AAGCTCAACT AAAGAGGCTT
    AAAAACAAAG CGGCAACCAC AGCAGCCCCT GTGCTCAAAC CCAAAGCCAA GGAGACCCAG
    AGAGCAAAGC CCCCCTCGGT GGGAGATACC AACGATGCCT TTCTCGTGAC CGATTCCGAC
    ACAGAGCCGC CCGCTGCTGT TGGTCAGACT GCGACAAAGT CCTGCACCAC ACGCCCCAAT
    GCCCGTCCCT GCAGCAAAGC ACGTGCACAG GGCTCTGTGG CATCCCCTGC ACGGAAACCC
    AGAGGGAAGA AGAAGCAGCA AGCCAAGAGC ACAGACACCG ACGATGCCTT CGCTTTGGGT
    GACTCGGACA GCAAGGTGCC TCCCATTGTG GTCCGGCCGG CAGCAGTGCC CTGCACTCCG
    CGCCAGAAGC CGTCCTGTCC TCTAGCCAAG ACATACACCC GGGGGGCTGC GATGTCCCTA
    CGGCCCAGGG CTCCCTTCAC CGTGTTCAGC GAGGCCTCGC CACCACTCAG CAAGTCACAG
    CTCCTGAAAG CACCCAAAGC CTGTGGGAAA ACCAGATCCC GGCTGCCGGT GACATTCAGT
    GATGACAGCG ACACCGAGGG TCTCAAATCA GTGCTGAGCC CCATTGCTGG CAAAGCACCC
    ATTGCCGGCT GCAAGACGCC GAAGTCAGTG TGCTGCTCAA AGAGAGCTCT GCTCCCCCGG
    TCCTTGGAGA TGGGGGGCTC CCAGCTGAGC TCCTCCTCCG TGGGCAGAGG CTGTAGGAGC
    AGCACCCGGC CCCGGCGGGG GCGCACAGCT GGGGGCACCG AGAAGAAGGA GCGGGCAGCA
    CGCAGGGCTC CGAGCTGGAA AGGGGATGAG GAACGGGAGC TGCTGTGTGC CATCGAAGAG
    AAGGAAAAGG TGGAGGAGCA GGTCAACGTC AGCTTGGAGC TACTCCAAGC CTCTGAGGAT
    GAAGCCCAGG GTGGAATCCA ACGCAGGCTG GAGGGCGCGG ACATGGAGCT CGAGGGGCTG
    CGGCAGGAGG TTGGTGGAGA GGTCCTGGCA CGGCAGAGGC TGGGCAGTAC GGAGCCCCTG
    TGCCTCAGCG CCCTGCACAA CGTGCTGCCA GCTGTGGATG ACGCCTCCTT GCTGGACACT
    GCCTGCGGGC TCCTGAAATC TGCCTTCAGC TGCATCAGCC ACTGCCCACC CGGCACGCTG
    TACAGCCAGC TCTGTCAGCT CCTGGCTCTG GCCACGGGTG ATCAGGACCC CATCACCACT
    GCCTACCTGC TGGCTGAGTC TGTCTCGGTC ACCACGCGCC ATCAGCTGCT CAGTATCATC
    CACAAGAAGC TTCATAAGGA GAAGAAAGCA GCAGCAGATG TGGCTGAGCA GCTCCAAGGG
    CTGACCCTGC AGGATAAGGA TGCTCCTTCA CACCACTGCC ACCTCACTGA GCTCCAGGAC
    CTCTTCAGCT TCAGCCCCAC GGGTGTGGAG CCCCAGGAGT GCACCAGCTT CAGTGAGCAG
    CTGCAGCAGA TCCCAGCTGG GGTGACTGTG TGCGTGCTGA CCCTGGTGAG CATCCAGCCC
    ACCTCCGTGG GGGACACACT GCTGCTGACA CGGTTGGAGA GGGGCAGCGC TCCCATCACT
    GTCCGCATCC CAACTGCGCA CACCAAGACC CCGCTGAGCT CAGTGCTGAG TGACTTCGAC
    TCCATCCAGA AGGAGCAGAA GGATGCCAAC GGCTACACTG AAAAGAGAGC CTGGTGGCTG
    TGCCGCTTCG AGCTGGACCA GAGGATGAAG AACCTCATTG AGACGCTGGA AATGCAGGTG
    CTGGGTTGCT GGAAGGCAGC GCTGCTCCCT GCAGCCCAGG ATCCCCTGCT GGCTGAGGAA
    GCTGCCCGTT TATGTGCTCA GCTGCAGCAG TGTGGCTGGT GGGGCAAAGA TCCGACCCTA
    CTCAAGGTGC TGCTGAACGC CGCTGCCCTC CTCAGCCCTG CTGATGTGCA AGCCCTGGCC
    GTGGGGCTGT GCCCAGCTCA GCCCAGCAGA GCCCGGCTCC TCTTGCAGGA GGCTCTGGAC
    AAGCAGAAAA ACAGCACACG GGAGCCGGGC GGTTCCGTCA TCCTGGTGCT GGATAAGCAC
    CTGCAGAAGC TGCCCTGGGA GAGCATGGCG TGCCTGAGGG ACCTGCCCGT CACCCGGCTC
    CCATCCCTGC GCTTCCTCCT CAGCTACACC CTGACACAGA GGCAGACAAA CTCCGTGCTG
    TGCCGCGGCA TTGACCCCAG CAGGACCTTC TATGTCCTCA ACCCTCAGAA CAACCTCTCG
    AGCACTGAGG AGTGTTTCCG CAGCTGGTTT GAGAGTGAAC CAGGCTGGAG AGGGGTGACA
    GGATCAGTCC CCAGCCAGGA GCAGATGCAG GCAGCGCTGC TGGAGCACGA TCTCTACATC
    TACGCGGGAC ACGGAGCCGG TGCCCACCTG CTGGACGGGC AGACCATCTC CCAGCTGCAG
    TGCCGTGCTG TCGCTCTGCT CTTCGGCTGC AGCAGCGCCG CCCTCGCCGT GCGTGGCAGC
    CTGGAGGGTA CGGGCATTGT GCTGAAGTAC ATCATGGCTG GCTGCCCCCT CGTCTTGGGC
    AACCTGTGGG ACGTCACCGA CCGCGACATT GACCGCTATG CTGAGGCACT GCTGCGTGCC
    TGGCTGGGGG CTCCTCGGCC CGGAGCCCCC CTTCTCCACT ACATGGCCGA GGCCCGGCGT
    GCCCCCCGCC TCCAGCACCT CATCGGGGCC GCCCCCGTGG CCTATGGGCT CCCTGTGGCC
    CTGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025001255.1
    CDS150..6818
    Translation

    Target ORF information:

    RefSeq Version XM_025145487.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025145487.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGAATCGCC TCAAAGGCAC CGACTTTGTG TCCCGCACTG CCAGCCCGGA GGGCACAGAG 
    GAGCTGCTCA GGGAGCTGTT GCAATGCCTC TCCAGCAGCA CGGTGGTGCC GGGCAGTGTG
    CAGAGCACGG TGTGCGACCG CGTCCTGCGG GCCTGCGTGC AGCGCCTGGC GGAGCCCGGC
    TGCGGCCTCA CGCACACCGA CGCGGTGCTG GCGTTGGCCG AGGCGGCCTG CAGGGGCTTC
    GTCACCGCCG CCACCGCCCC GCCGAGCCCT CTCTACCTGG AGAAGATCCT CTACTACCTG
    CTGAAGAACG CGGCTGCCCG CGGCCATCGC AGTGCCTGCA GGCGGGCGGC GGAGCTCCTC
    CACGCTCAGC TCTGCGCCCA CCAGCAGAGC CACGCGCCGT CCGCCGACTT CGGTGCTGTT
    GTCCATAGCA GCTTCTGCAC GCTGTGGGGC GCCGCGGGGT CGCTGGGGGA CCGCCTGCAG
    GCCCTGCGCT TCCTGCTGCT GCTGGAGGAC GAGGAGGGGG CCGTGCTGCC CCTGGAGCCG
    CCCTTCTTCA CCTCGCAGAC CGCCCGGCAG GCGGTGTTGG CCGCCACTCT CTCCAAAGCC
    CACCACGCTC CATCCTCGGC TTGCTTTGGC CGGGAGCTGA GGGGTATCCT GACCTCCCTG
    CGCGAGGGCG CGGCGCGGCC CCCCTCCCTG CCCCACGCCC TCTGCTTCTT GGAGCTGGCG
    TTGGAGCAGT GCCGGCAGCT CTGCCAGAGC GGCTGCCACG GGGAGGCGGA GGCGGCGGTG
    AGGGATGCGC GAGGATTCGT GGGGGGCTCT GAGGCGTTCG GCGAGGCCCT GGCCCTGCTG
    GAGGCCGGCG TGCAGCTGAG CCGGGCGCTG GCCCCGAACG GGGGCTCAGC AGGGCCGGCC
    CTGGCGCAGG CGGCGGCAGC GCTGGGCGGC GCAGCGAAGG CCTCGGGGCG CTTCCTCAGG
    GTGCTGGCCG AGGGCTGCCA GTTCGTCACC GCGTCCCTCC ACCAGCGTGC CAGGAGCAGC
    CAGCAGCAGC CGATGCCTCG GGACGACGTG CTCCAGCTCT GCGCCTTTGT GCGGGGGCAC
    TGCCGTGTCC TCGGCCTGCT GCTGCGCGTG GCGCCTCCTG ACGGCGTGAA GCAGCGGCTC
    GTGCTGAAGC AGCTGCTGTA CCACAGCCTC CAGCTCTTCA CCAGCGTGGC CTGTGACACC
    TTCCGCTCCT CCCAGGTGGC CGGGTGGTCA GGGCAGGAAC GGCTGACATT GGGCTGCCAG
    GACACCGTGA TGCAGATGCT GGAGGCTCTG GAGGGACTTC CCCAGAGGGA GCAAGCCAAG
    TACCTCGACA TTACCGCATC GTGCACGTTT AAGCTGGCAT ACATCTTCTA CAGCCAGAAC
    CTTTACAAGG AAGCCAGCTC CGTCTCCGAG CTCTTCTGCA AGAGGCTGAA GCAAATAGAT
    GCCTTCAGAT TTCCAGAGAT ACCTCCCGAA AAGCTGCACA GGTGCTTCAG GCTGCAGGTG
    GAAAGCTACC AAAAGCTGGG CCTGCTGGAG AGCGCGCTGG CGTCCTGCGT GCAGTGGCTG
    ACGTCGCTGC GGGGCCGCGT TGGGGAGCTG CTGGCGGAGC CCATCGGCCT CTGGGCCCGA
    GTGAAAATGG ACAGCGTGAA GCAGGGAGCG GAGGACTTAC GGCTGAGAAC CCTGAAGGAA
    GAACTGTCTG CTCACCACCT GGACGCGGAC ACCTTGGTGG CTGTGCTCTT TGCCGAGCTG
    AAGGCTTACA AGAGCATCCG TGCCGACACA GGGCAGGAAC GCTACAACGT GCTCTGCGAC
    CTGCTGGAGA TCTGCTCCGA GGACAGCGGC CGCCTGCATG AGCGCGCTGC CTGCTTGGTG
    GAGCTGGCCC AGGTCCTTTG CTACCACAAC TATGCCCAGC AAACCGACTG CTCCGCGTTG
    GATTCTGTCC ACGAAGCACT GCGGCTGTTG GATTCGGTGC CCCGGAGTGC TGAGAACCGG
    GAGCAGCTCC TCGATGACCG GGCGCAGGCT CTGCTCTGGC TCTACATCTG CACCCTGGAG
    TCCAAGCTGG AGGAGGGCAT AGCAAGGGAG CAGAGAGTCA AAGCTCTGGG ACAGAAGAGC
    CTGGAAGACT TTGAGCCCAA TGACCTCAAC TATGAAAGCA GGCTGCAGGA GGATGCATTC
    CTGAACAGCG GCATCTCCTT CAACCTGCTG ACAGAATTGG CTCAGTCCAA GAGCCTGGAC
    GATGCCTTAG CCCTATGGAA GCAGCTGCTG GCATCTCAGG GCATCCCAGC TGTGCGCAGT
    GTGGAGCAGA CCACGGCCTC TCTTCGTGTT ACAGCAGCTC TCTACAGGCT GATGGACAAG
    CCTCTGCAAG CCATGGAAAG CTACCTGCTG GTGCGAGCCC TGTGCAGCGC ACTTGGGGAC
    AATTTGGGCA CGGCCGGAGC TCTCTGCCAG GTCACCAAAC TGCTCCTCCA GCTGGAATGC
    CCCAGCTACG CCAAGCTCTT CCTGGAAGAG GCAGAGTCCT GCTTGCAGAG AACAGACGGG
    GGCAGCGACT CCTACCTGCT GCTGCAGCAG ACCTGCCTCG TGCTCCGCAG CCAGCTGTAC
    TGCGCCAGCC ACCGCATTAA AGATGGCACT GCCCTCTTGC TGGAGGTGCT CCAGAACCCG
    GCTCTGCAGA AGACTGCCAA GGTCTGGTAC ATGCTCCAAG CACAAGTCCT GCAACTGACC
    GCCACCTACC TGAGCCTGCC GCCCAGCCAC CTCTCGCCAG AGTTCAGGCA GTACATCTTC
    ACGCAGGGGT GGAAGACTCC CGAGACAGCT CTGAGCGAAG CCCACAGGTT GTTACGTGGC
    ATCGTCCTCC TCCTGATGGG CAGCAACGTG CTGGGCTCCC ACAAGTCAGC AGCAGATATC
    CGGTTCATAG ATTGTGGGGA CAACGTACTG CAGAAGTGGC AAGTGCTGGC CGACTTGCTG
    GGCTGCTCAG AGCACCTGGT GGTCCTCCTC AGCAGGGTGG AGGTGATGTG CAAAGCCAAA
    GCCTTCTGCT TGGAGGCCAT CAAGCTGGCT GTGAAACTGC AGGCGGTCCG ATGGTGTGCA
    TCCTTCCTGG TGCTGAAGGC TCAGCTGGAG CTGCAGCAGA GTGAGCTGGA GCTGAGCCAC
    TGGAACCTGC AGCAGGCTCT GTTCCTCCTG GAGTCCGGCA CCGAGTTTGA GGCTGATGAG
    AAGCAGCGAG CCCCCCTGAA GATCTTGCCC AGGAAAGGCA AACCAGAGGG CAGGAAGCAG
    CGGGATGAGG GCTCTGAGCT CCCGGGAGAA GATGAGGGCT TTCTGAAGGG CCCTGCGCTG
    GAGTTTGTGG CCATGGTGAG CGGCCTGGAG AAAGCAGATG ACCTCAGCTC CTCGCCACAG
    CTGAAACCCA AGAGGAAGAG GAGGCTCGCC TTCCTCGCCC ACCCCGCTGC CTGCCCCTGC
    TGCCTCTGCT CCGACCTGAC CCTGTCGTCC CTCTGCCTCC GCTGGCTGCT CTGCTGTGCG
    CGGGGTGAGC TGGCGGCCGG CAGTGCAGCC GAAGGGCTGG GGCTGATCCA AGCCACCCTG
    CAGCGCTGCG GCACCGTGGG CCCTCGCTTC ACCGTCCTGC TGCAGGACAA GCTGGGCAGT
    GGTCACAAGG CACCTGCACT GCGGCTGTTA GATGATTTGG TGGCCACTGG CTATGCCGCA
    CTGGCCCTTC ACAGCCTGGC TGGTCTCTGT CCTGAGGAGA AGCTGGAGGA GCACCTGAAA
    GCCGGTCTGT CTTTCCTGGC ATCCTGCAGC CTCCACCTGC CCAGCCTGGG GGTCTCCAAG
    GCCAGCCTGC TGCTTGCCAA AGCCACGGCT GCTGTTTGCC GCTTGGCCGC CAAGCACGGA
    GGCTCTGTGG ACGCTGTCTT TGCCTCTGCT TGGGCTTGGC AGCCAGCCCC ACTGAGCCCC
    ACCGAGCCCA AAGCAGCAGC GGCTGCGCCC AGGATGGACA AAGCTCAACT AAAGAGGCTT
    AAAAACAAAG CGGCAACCAC AGCAGCCCCT GTGCTCAAAC CCAAAGCCAA GGAGACCCAG
    AGAGCAAAGC CCCCCTCGGT GGGAGATACC AACGATGCCT TTCTCGTGAC CGATTCCGAC
    ACAGAGCCGC CCGCTGCTGT TGGTCAGACT GCGACAAAGT CCTGCACCAC ACGCCCCAAT
    GCCCGTCCCT GCAGCAAAGC ACGTGCACAG GGCTCTGTGG CATCCCCTGC ACGGAAACCC
    AGAGGGAAGA AGAAGCAGCA AGCCAAGAGC ACAGACACCG ACGATGCCTT CGCTTTGGGT
    GACTCGGACA GCAAGGTGCC TCCCATTGTG GTCCGGCCGG CAGCAGTGCC CTGCACTCCG
    CGCCAGAAGC CGTCCTGTCC TCTAGCCAAG ACATACACCC GGGGGGCTGC GATGTCCCTA
    CGGCCCAGGG CTCCCTTCAC CGTGTTCAGC GAGGCCTCGC CACCACTCAG CAAGTCACAG
    CTCCTGAAAG CACCCAAAGC CTGTGGGAAA ACCAGATCCC GGCTGCCGGT GACATTCAGT
    GATGACAGCG ACACCGAGGG TCTCAAATCA GTGCTGAGCC CCATTGCTGG CAAAGCACCC
    ATTGCCGGCT GCAAGACGCC GAAGTCAGTG TGCTGCTCAA AGAGAGCTCT GCTCCCCCGG
    TCCTTGGAGA TGGGGGGCTC CCAGCTGAGC TCCTCCTCCG TGGGCAGAGG CTGTAGGAGC
    AGCACCCGGC CCCGGCGGGG GCGCACAGCT GGGGGCACCG AGAAGAAGGA GCGGGCAGCA
    CGCAGGGCTC CGAGCTGGAA AGGGGATGAG GAACGGGAGC TGCTGTGTGC CATCGAAGAG
    AAGGAAAAGG TGGAGGAGCA GGTCAACGTC AGCTTGGAGC TACTCCAAGC CTCTGAGGAT
    GAAGCCCAGG GTGGAATCCA ACGCAGGCTG GAGGGCGCGG ACATGGAGCT CGAGGGGCTG
    CGGCAGGAGG TTGGTGGAGA GGTCCTGGCA CGGCAGAGGC TGGGCAGTAC GGAGCCCCTG
    TGCCTCAGCG CCCTGCACAA CGTGCTGCCA GCTGTGGATG ACGCCTCCTT GCTGGACACT
    GCCTGCGGGC TCCTGAAATC TGCCTTCAGC TGCATCAGCC ACTGCCCACC CGGCACGCTG
    TACAGCCAGC TCTGTCAGCT CCTGGCTCTG GCCACGGGTG ATCAGGACCC CATCACCACT
    GCCTACCTGC TGGCTGAGTC TGTCTCGGTC ACCACGCGCC ATCAGCTGCT CAGTATCATC
    CACAAGAAGC TTCATAAGGA GAAGAAAGCA GCAGCAGATG TGGCTGAGCA GCTCCAAGGG
    CTGACCCTGC AGGATAAGGA TGCTCCTTCA CACCACTGCC ACCTCACTGA GCTCCAGGAC
    CTCTTCAGCT TCAGCCCCAC GGGTGTGGAG CCCCAGGAGT GCACCAGCTT CAGTGAGCAG
    CTGCAGCAGA TCCCAGCTGG GGTGACTGTG TGCGTGCTGA CCCTGGTGAG CATCCAGCCC
    ACCTCCGTGG GGGACACACT GCTGCTGACA CGGTTGGAGA GGGGCAGCGC TCCCATCACT
    GTCCGCATCC CAACTGCGCA CACCAAGACC CCGCTGAGCT CAGTGCTGAG TGACTTCGAC
    TCCATCCAGA AGGAGCAGAA GGATGCCAAC GGCTACACTG AAAAGAGAGC CTGGTGGCTG
    TGCCGCTTCG AGCTGGACCA GAGGATGAAG AACCTCATTG AGACGCTGGA AATGCAGGTG
    CTGGGTTGCT GGAAGGCAGC GCTGCTCCCT GCAGCCCAGG ATCCCCTGCT GGCTGAGGAA
    GCTGCCCGTT TATGTGCTCA GCTGCAGCAG TGTGGCTGGT GGGGCAAAGA TCCGACCCTA
    CTCAAGGTGC TGCTGAACGC CGCTGCCCTC CTCAGCCCTG CTGATGTGCA AGCCCTGGCC
    GTGGGGCTGT GCCCAGCTCA GCCCAGCAGA GCCCGGCTCC TCTTGCAGGA GGCTCTGGAC
    AAGCAGAAAA ACAGCACACG GGAGCCGGGC GGTTCCGTCA TCCTGGTGCT GGATAAGCAC
    CTGCAGAAGC TGCCCTGGGA GAGCATGGCG TGCCTGAGGG ACCTGCCCGT CACCCGGCTC
    CCATCCCTGC GCTTCCTCCT CAGCTACACC CTGACACAGA GGCAGACAAA CTCCGTGCTG
    TGCCGCGGCA TTGACCCCAG CAGGACCTTC TATGTCCTCA ACCCTCAGAA CAACCTCTCG
    AGCACTGAGG AGTGTTTCCG CAGCTGGTTT GAGAGTGAAC CAGGCTGGAG AGGGGTGACA
    GGATCAGTCC CCAGCCAGGA GCAGATGCAG GCAGCGCTGC TGGAGCACGA TCTCTACATC
    TACGCGGGAC ACGGAGCCGG TGCCCACCTG CTGGACGGGC AGACCATCTC CCAGCTGCAG
    TGCCGTGCTG TCGCTCTGCT CTTCGGCTGC AGCAGCGCCG CCCTCGCCGT GCGTGGCAGC
    CTGGAGGGTA CGGGCATTGT GCTGAAGTAC ATCATGGCTG GCTGCCCCCT CGTCTTGGGC
    AACCTGTGGG ACGTCACCGA CCGCGACATT GACCGCTATG CTGAGGCACT GCTGCGTGCC
    TGGCTGGGGG CTCCTCGGCC CGGAGCCCCC CTTCTCCACT ACATGGCCGA GGCCCGGCGT
    GCCCCCCGCC TCCAGCACCT CATCGGGGCC GCCCCCGTGG CCTATGGGCT CCCTGTGGCC
    CTGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.