MED12L gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_015291831.1	XP_015147317.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X4
XM_015291834.2	XP_015147320.2	mediator of RNA polymerase II transcription subunit 12-like protein isoform X5
XM_025153451.1	XP_025009219.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X8
XM_015291836.1	XP_015147322.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X9
XM_015291830.2	XP_015147316.2	mediator of RNA polymerase II transcription subunit 12-like protein isoform X3
XM_015291836.2	XP_015147322.2	mediator of RNA polymerase II transcription subunit 12-like protein isoform X7
XM_015291833.1	XP_015147319.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X6
XM_025153452.1	XP_025009220.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X9
XM_015291828.2	XP_015147314.2	mediator of RNA polymerase II transcription subunit 12-like protein isoform X2
XM_015291828.1	XP_015147314.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X1
XM_015291830.1	XP_015147316.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X3
XM_015291832.1	XP_015147318.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X5
XM_015291834.1	XP_015147320.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X7
XM_015291835.1	XP_015147321.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X8
XM_015291837.1	XP_015147323.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X10
XM_015291831.2	XP_015147317.2	mediator of RNA polymerase II transcription subunit 12-like protein isoform X4
XM_015291835.2	XP_015147321.2	mediator of RNA polymerase II transcription subunit 12-like protein isoform X6
XM_015291829.1	XP_015147315.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X2
XM_025153450.1	XP_025009218.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa152700	XM_025153451.1 Latest version!	Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa152701	XM_025153452.1 Latest version!	Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa152693	XM_025153450.1 Latest version!	Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa152694	XM_015291828.2 Latest version!	Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa152695	XM_015291830.2 Latest version!	Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa152696	XM_015291831.2 Latest version!	Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa152697	XM_015291834.2 Latest version!	Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa152698	XM_015291835.2 Latest version!	Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa152699	XM_015291836.2 Latest version!	Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa40175	XM_015291836.1 Latest version!	Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa40167	XM_015291828.1 Latest version!	Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OGa40168	XM_015291829.1 Latest version!	Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OGa40169	XM_015291830.1 Latest version!	Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OGa40170	XM_015291831.1 Latest version!	Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OGa40171	XM_015291832.1 Latest version!	Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OGa40172	XM_015291833.1 Latest version!	Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OGa40173	XM_015291834.1 Latest version!	Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OGa40174	XM_015291835.1 Latest version!	Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OGa40176	XM_015291837.1 Latest version!	Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

MED12L ( XM_025153451.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025009219.1 Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OGa152700
Clone ID Related Accession (Same CDS sequence)	XM_025153451.1
Accession Version	XM_025153451.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAGCTGCTG 
CTGAAGTCGT CCAGCCTTGC AGGGAGCTAC ACCACGGGGC TGTGTGTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCT AAGGTGAAGC AAACGATATA CAGCAATGTT 
CAGCCTTCAA ATTCCAATCT GCTGTGGGAC CCGGAGTTCA TGCTTGATTT TATTGAGAAC 
CCTTCTGCTC ACAGCATTAA CTACTCCATG CTGGGCAAGA TCCTGAGTGA CAACGCAGCC 
AACCGCTACA GCTTTGTCTG CAATGCTCTA ATGAATGTGT GCATGGGGCA CCAAGATGCA 
GGGAGGATTA ATGACATAGC AAACCTTTGT GCAGAGCTGA CGGCGTGCTG CACCGTGCTG 
AGCTCGGAGT GGCTGGGGGT TGTCAAAGCT CTTTGCTGCT CTTCCAATCA CGTGTGGGGT 
TTCAATGATC TGCTCTGCAG CGTGGATGTG AGTGACCTGT CATTCCACGA TTCCCTGGCC 
ACTTTCATTG CCATACTGAT TGCCCGGCAG TGCTTTTCTC TGGAAGATGT CGTTCAGCAC 
GTAGCACTGC CTTCTCTTCT TGCAGCTGCC TGTGGGGACC CAGATGCTGA GCCTGGGGCC 
AGGATGACTT GTCGGCTTCT CCTGCACCTC TTCAGGACAC CCCAGGTCTG CCTCTTTCCC 
CAGGGAGCAG GAAAATTATT TCCTGGAATT CGTTCTTCTT GTGATCGTCA CCTCTTGGCT 
GCTGCTCACA ACAGCATAGA AGTGGGAGCT GTGTTTGCAG TCTTAAAAGC AATTTTGATG 
CTTGGTGACG CTGAGATCGG CAGCAGCAGT GTCACCCCTG TGAAGAACGA GGAGCTGCCC 
GTGAGGGGCT TGCTGGATGA GATCGCTGAG GATGAAATGT GGGCTTCCTC TCACGCTCTG 
AAATCACGTG GAAAAGCTGT TTCCATAGAA ACCGCCAGTT TGAGCGAGTA TGCTAGATAT 
GTGCTAAGGA CCATTTGCCA GCAGGAATGG GTTGGAGAGC ACTGTTTAAA AGAACCTGAG 
AGGCTGTGCA CAGATAAAGA TCTAATTTTG GATCCCGTTC TTTCAAACAA GCAAGCACAG 
AAACTTCTTC AGCTTATCTG TTATCCTCAT GGTGTGAAAG AATGCTCCGA GGGGGACAAC 
CCTCAGAGGC AGCACATCAA ACGCATACTT CAGAACTTAG ACCAGTGGAC TCTGCGGCAG 
TCTTGGTTGG AGCTGCAACT AATGATCAAG CAGTGCATGA AGGAGCCTGG TTCTGGGTCT 
GTGGCTGAAA TGAACAGCTT GTTGGATAAT ATTGCAAAGG CGACAATAGA GGTGTTTCAG 
CAATCCGCTG ATCTAAACAA TAACTCTTCT AACTCTGGTA TAGGCCTCTT CAATCCAAAC 
TCTGTTGGAA ATGCTGACAC AAGTAATACA AGACAGAATG GTAAAAAGAC ATTCCTAAGT 
TCCTCCGAGT GGCGAGGAGT GTGGCTGGTG GCTCCCTTGA TTGCAAAACT ACCTACCTCA 
GTTCAAGGTA GGGTCTTGAA AGCTGCGGGA GAGGAGCTGG AGAAAGGTCA GCATTTGGGG 
TCATCTTCTA AGAAGGAAAG AGATAGACAG AAGCAAAAGA GCATGTCTCT CTTGAGTCAG 
CAGCCTTTCC TTTCTTTGGT ACTTACTTGC CTTAAGGGAC AGGATGAGCA GCGGGAAGGG 
CTTCTAACAT CACTGCAGAA TCAAGTCAAT CAGATCCTTA GTAACTGGAG GGAAGAACGG 
TACCAGGGTG ATGTTAAAGC TAGGCAAATG ATGCATGAAG CCTTACAGCT TCGTCTGAAT 
TTGGTAGGTG GCATGTTTGA TACTGTGCAG AGGAGTACCC AGTGGACCAC AGACTGGGCA 
CTTCTGCTCC TCCAGATAAT CACTTCGGGA ACAGTTGATA TGCAGACCAA CAACGAATTG 
TTCACAACCG TGCTGGATAT GCTGGGTGTC CTCATCAACG GGACGCTCGC CTCCGACTTG 
TCCAGTGCTT CCCAAGGGGG ACCCGAGGAA AACAAAAGGG CTTACATGAA CTTAGTGAAA 
AAGTTAAAAA AGGAGCTGGG AGACAAGCGG TCAGACAGCA TTGACAAGGT TCGCCAGCTG 
CTCCCTTTGC CAAAGCAGAC ATGTGATATT ATTACCTGTG AGCCAATGGG ATCCTTGATT 
GACACCAAGG GCAACAAAAT TGCGGGGTTT GACTCCATTG ATAAGAAACA GGGTCTTCAG 
GTGTCAACAA AACAAAAGGT GTCCCCTTGG GATTTGTTTG AAGGTCATAA AAATCCTGCT 
CCTCTCTCCT GGGCCTGGTT TGGTACAGTT CGTGTCGACA GGAAGGTGAT CAAATACGAA 
GAGCAGCAGC ATCTGCTCCT GTACCATGCG CACCCCAAAC CCAAGCCGCG GAGCTACTAC 
CTCGAGCCCC TGCCTCTGCC TCCGGAGGAG GAGGAGGAGG AGGAGCCCAC CACCCCTGTC 
TCTCAGGAAC CAGAAAGGAA GTCAGGAGAT CAGGATCAGG GAAAGCATGC CACAGATGAC 
GAGAAGAAGA CAAAGGGCAG GAAGAGGAAG TCAAAGTCAG GCTCTAAGGC TGATGAATAT 
CCACAGAACA ACTTATACAG AGTGCCTCCC AATTACTCTC CCATTCCCTC ACAAATGATG 
CAGCATCCTC AGTCTGCCCT GTGGGGCTAC ACCATCATGG GGCAGCCGCA GCAGCCTGGC 
TTCTTCGTGC AGAACCAGCC CCTCCCTCCA GGTGGTTCCA GGCTGGATCC AACGAGCTCC 
TTCGTCCCAG CCAACACGAA GCAGGCCCTG TCCAACATGC TGCAGCGGCG CTCCGGCACC 
GTGCTGCAGC CCCCTGCCCT CCACGCAGTC ACACCTCAGC AGCAGTTGCT CCAGATGAAA 
CTCCTGCAGC AAGAGCAGCA GCAGCAGCGG CTCCTCAGGC ACCAAGCACA GGCACGGTCT 
CTCCAGCAGG ATGTCAGTAG CTCTGCCGTA TCCCTTCAGG ATGGCTTTGA CAACATAATG 
GGTCAGCCGA TGGACCCCGC TGCTCTTTTC ACCCCGCAAG TCCGACCCAC GCCACAGCTA 
CCGCAGTACC CAGGGCTTCA GCAAGCACAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025009219.1
CDS	33..6005
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPRQKEDELTAVNVKQGFSNQPAFSGDEHGSARNIVINASKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHNWFTDLAGSKPLTILAKKVPILSKKEDVFAYLAKYSVPLLRAAWLIKMTCAYYAAISEAKIKKRQATDPNIEWTQIITRYLREQLAKVAEFYHVTSSQGNSSVVMPQEMEQALKQWEYNEKLSFYMFQEGMLERHEYLTWILDVLEKIRPTDDDILKLVLPLMLQYSEEFVQSAYLSRRLAYFCARRLSLLLSDGPSLAAAHSPHVIIAPSNPPLAVPSPTAPGPVLSPVQLTCSDFLSCPQHRPLVYGLSCMLQTITLCCPSALVWNYSTNENKNMNPGSPLDLLQVAPSSLPMPGGNSTFNQQVRAKIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRSDSSNSMETLYHKIFWANQNKDNQEAAPSDEAVVMLLCEWAVSCKRSGKHRAMVVAKLLEKRQAEIEAERCGEAEVLDEKESLSSASLTSSSLPVFQNILLRFLDTQAPSLSDPNSDHEKTEFVNLVLLFSEFTRHDVFSHDAYMCTLISRGDLSITATTRPRSPNGETGDEHYSKDHDMKMEDHNIMEHMGTDSGPTSIFDDVDKGDFKADFGSEFPIFSPMPGESCENMNSSLDRRISVASEKSVKRERLREVIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRMILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQEAFPTLETVFTKLQQLSYFDQHQVTSQISSNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHACLILNSEQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYSNVQPSNSNLLWDPEFMLDFIENPSAHSINYSMLGKILSDNAANRYSFVCNALMNVCMGHQDAGRINDIANLCAELTACCTVLSSEWLGVVKALCCSSNHVWGFNDLLCSVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDPDAEPGARMTCRLLLHLFRTPQVCLFPQGAGKLFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAILMLGDAEIGSSSVTPVKNEELPVRGLLDEIAEDEMWASSHALKSRGKAVSIETASLSEYARYVLRTICQQEWVGEHCLKEPERLCTDKDLILDPVLSNKQAQKLLQLICYPHGVKECSEGDNPQRQHIKRILQNLDQWTLRQSWLELQLMIKQCMKEPGSGSVAEMNSLLDNIAKATIEVFQQSADLNNNSSNSGIGLFNPNSVGNADTSNTRQNGKKTFLSSSEWRGVWLVAPLIAKLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQGDVKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMQTNNELFTTVLDMLGVLINGTLASDLSSASQGGPEENKRAYMNLVKKLKKELGDKRSDSIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGHKNPAPLSWAWFGTVRVDRKVIKYEEQQHLLLYHAHPKPKPRSYYLEPLPLPPEEEEEEEPTTPVSQEPERKSGDQDQGKHATDDEKKTKGRKRKSKSGSKADEYPQNNLYRVPPNYSPIPSQMMQHPQSALWGYTIMGQPQQPGFFVQNQPLPPGGSRLDPTSSFVPANTKQALSNMLQRRSGTVLQPPALHAVTPQQQLLQMKLLQQEQQQQRLLRHQAQARSLQQDVSSSAVSLQDGFDNIMGQPMDPAALFTPQVRPTPQLPQYPGLQQAQ

Target ORF information:

RefSeq Version	XM_025153451.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025153451.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAGCTGCTG 
CTGAAGTCGT CCAGCCTTGC AGGGAGCTAC ACCACGGGGC TGTGTGTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCT AAGGTGAAGC AAACGATATA CAGCAATGTT 
CAGCCTTCAA ATTCCAATCT GCTGTGGGAC CCGGAGTTCA TGCTTGATTT TATTGAGAAC 
CCTTCTGCTC ACAGCATTAA CTACTCCATG CTGGGCAAGA TCCTGAGTGA CAACGCAGCC 
AACCGCTACA GCTTTGTCTG CAATGCTCTA ATGAATGTGT GCATGGGGCA CCAAGATGCA 
GGGAGGATTA ATGACATAGC AAACCTTTGT GCAGAGCTGA CGGCGTGCTG CACCGTGCTG 
AGCTCGGAGT GGCTGGGGGT TGTCAAAGCT CTTTGCTGCT CTTCCAATCA CGTGTGGGGT 
TTCAATGATC TGCTCTGCAG CGTGGATGTG AGTGACCTGT CATTCCACGA TTCCCTGGCC 
ACTTTCATTG CCATACTGAT TGCCCGGCAG TGCTTTTCTC TGGAAGATGT CGTTCAGCAC 
GTAGCACTGC CTTCTCTTCT TGCAGCTGCC TGTGGGGACC CAGATGCTGA GCCTGGGGCC 
AGGATGACTT GTCGGCTTCT CCTGCACCTC TTCAGGACAC CCCAGGTCTG CCTCTTTCCC 
CAGGGAGCAG GAAAATTATT TCCTGGAATT CGTTCTTCTT GTGATCGTCA CCTCTTGGCT 
GCTGCTCACA ACAGCATAGA AGTGGGAGCT GTGTTTGCAG TCTTAAAAGC AATTTTGATG 
CTTGGTGACG CTGAGATCGG CAGCAGCAGT GTCACCCCTG TGAAGAACGA GGAGCTGCCC 
GTGAGGGGCT TGCTGGATGA GATCGCTGAG GATGAAATGT GGGCTTCCTC TCACGCTCTG 
AAATCACGTG GAAAAGCTGT TTCCATAGAA ACCGCCAGTT TGAGCGAGTA TGCTAGATAT 
GTGCTAAGGA CCATTTGCCA GCAGGAATGG GTTGGAGAGC ACTGTTTAAA AGAACCTGAG 
AGGCTGTGCA CAGATAAAGA TCTAATTTTG GATCCCGTTC TTTCAAACAA GCAAGCACAG 
AAACTTCTTC AGCTTATCTG TTATCCTCAT GGTGTGAAAG AATGCTCCGA GGGGGACAAC 
CCTCAGAGGC AGCACATCAA ACGCATACTT CAGAACTTAG ACCAGTGGAC TCTGCGGCAG 
TCTTGGTTGG AGCTGCAACT AATGATCAAG CAGTGCATGA AGGAGCCTGG TTCTGGGTCT 
GTGGCTGAAA TGAACAGCTT GTTGGATAAT ATTGCAAAGG CGACAATAGA GGTGTTTCAG 
CAATCCGCTG ATCTAAACAA TAACTCTTCT AACTCTGGTA TAGGCCTCTT CAATCCAAAC 
TCTGTTGGAA ATGCTGACAC AAGTAATACA AGACAGAATG GTAAAAAGAC ATTCCTAAGT 
TCCTCCGAGT GGCGAGGAGT GTGGCTGGTG GCTCCCTTGA TTGCAAAACT ACCTACCTCA 
GTTCAAGGTA GGGTCTTGAA AGCTGCGGGA GAGGAGCTGG AGAAAGGTCA GCATTTGGGG 
TCATCTTCTA AGAAGGAAAG AGATAGACAG AAGCAAAAGA GCATGTCTCT CTTGAGTCAG 
CAGCCTTTCC TTTCTTTGGT ACTTACTTGC CTTAAGGGAC AGGATGAGCA GCGGGAAGGG 
CTTCTAACAT CACTGCAGAA TCAAGTCAAT CAGATCCTTA GTAACTGGAG GGAAGAACGG 
TACCAGGGTG ATGTTAAAGC TAGGCAAATG ATGCATGAAG CCTTACAGCT TCGTCTGAAT 
TTGGTAGGTG GCATGTTTGA TACTGTGCAG AGGAGTACCC AGTGGACCAC AGACTGGGCA 
CTTCTGCTCC TCCAGATAAT CACTTCGGGA ACAGTTGATA TGCAGACCAA CAACGAATTG 
TTCACAACCG TGCTGGATAT GCTGGGTGTC CTCATCAACG GGACGCTCGC CTCCGACTTG 
TCCAGTGCTT CCCAAGGGGG ACCCGAGGAA AACAAAAGGG CTTACATGAA CTTAGTGAAA 
AAGTTAAAAA AGGAGCTGGG AGACAAGCGG TCAGACAGCA TTGACAAGGT TCGCCAGCTG 
CTCCCTTTGC CAAAGCAGAC ATGTGATATT ATTACCTGTG AGCCAATGGG ATCCTTGATT 
GACACCAAGG GCAACAAAAT TGCGGGGTTT GACTCCATTG ATAAGAAACA GGGTCTTCAG 
GTGTCAACAA AACAAAAGGT GTCCCCTTGG GATTTGTTTG AAGGTCATAA AAATCCTGCT 
CCTCTCTCCT GGGCCTGGTT TGGTACAGTT CGTGTCGACA GGAAGGTGAT CAAATACGAA 
GAGCAGCAGC ATCTGCTCCT GTACCATGCG CACCCCAAAC CCAAGCCGCG GAGCTACTAC 
CTCGAGCCCC TGCCTCTGCC TCCGGAGGAG GAGGAGGAGG AGGAGCCCAC CACCCCTGTC 
TCTCAGGAAC CAGAAAGGAA GTCAGGAGAT CAGGATCAGG GAAAGCATGC CACAGATGAC 
GAGAAGAAGA CAAAGGGCAG GAAGAGGAAG TCAAAGTCAG GCTCTAAGGC TGATGAATAT 
CCACAGAACA ACTTATACAG AGTGCCTCCC AATTACTCTC CCATTCCCTC ACAAATGATG 
CAGCATCCTC AGTCTGCCCT GTGGGGCTAC ACCATCATGG GGCAGCCGCA GCAGCCTGGC 
TTCTTCGTGC AGAACCAGCC CCTCCCTCCA GGTGGTTCCA GGCTGGATCC AACGAGCTCC 
TTCGTCCCAG CCAACACGAA GCAGGCCCTG TCCAACATGC TGCAGCGGCG CTCCGGCACC 
GTGCTGCAGC CCCCTGCCCT CCACGCAGTC ACACCTCAGC AGCAGTTGCT CCAGATGAAA 
CTCCTGCAGC AAGAGCAGCA GCAGCAGCGG CTCCTCAGGC ACCAAGCACA GGCACGGTCT 
CTCCAGCAGG ATGTCAGTAG CTCTGCCGTA TCCCTTCAGG ATGGCTTTGA CAACATAATG 
GGTCAGCCGA TGGACCCCGC TGCTCTTTTC ACCCCGCAAG TCCGACCCAC GCCACAGCTA 
CCGCAGTACC CAGGGCTTCA GCAAGCACAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_025153452.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025009220.1 Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X9, mRNA.

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CloneID	OGa152701
Clone ID Related Accession (Same CDS sequence)	XM_025153452.1
Accession Version	XM_025153452.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAGCTGCTG 
CTGAAGTCGT CCAGCCTTGC AGGGAGCTAC ACCACGGGGC TGTGTGTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCT AAGGTGAAGC AAACGATATA CAGCAATGTT 
CAGCCTTCAA ATTCCAATCT GCTGTGGGAC CCGGAGTTCA TGCTTGATTT TATTGAGAAC 
CCTTCTGCTC ACAGCATTAA CTACTCCATG CTGGGCAAGA TCCTGAGTGA CAACGCAGCC 
AACCGCTACA GCTTTGTCTG CAATGCTCTA ATGAATGTGT GCATGGGGCA CCAAGATGCA 
GGGAGGATTA ATGACATAGC AAACCTTTGT GCAGAGCTGA CGGCGTGCTG CACCGTGCTG 
AGCTCGGAGT GGCTGGGGGT TGTCAAAGCT CTTTGCTGCT CTTCCAATCA CGTGTGGGGT 
TTCAATGATC TGCTCTGCAG CGTGGATGTG AGTGACCTGT CATTCCACGA TTCCCTGGCC 
ACTTTCATTG CCATACTGAT TGCCCGGCAG TGCTTTTCTC TGGAAGATGT CGTTCAGCAC 
GTAGCACTGC CTTCTCTTCT TGCAGCTGCC TGTGGGGACC CAGATGCTGA GCCTGGGGCC 
AGGATGACTT GTCGGCTTCT CCTGCACCTC TTCAGGACAC CCCAGGTCTG CCTCTTTCCC 
CAGGGAGCAG GAAAATTATT TCCTGGAATT CGTTCTTCTT GTGATCGTCA CCTCTTGGCT 
GCTGCTCACA ACAGCATAGA AGTGGGAGCT GTGTTTGCAG TCTTAAAAGC AATTTTGATG 
CTTGGTGACG CTGAGATCGG CAGCAGCAGT GTCACCCCTG TGAAGAACGA GGAGCTGCCC 
GTGAGGGGCT TGCTGGATGA GATCGCTGAG GATGAAATGT GGGCTTCCTC TCACGCTCTG 
AAATCACGTG GAAAAGCTGT TTCCATAGAA ACCGCCAGTT TGAGCGAGTA TGCTAGATAT 
GTGCTAAGGA CCATTTGCCA GCAGGAATGG GTTGGAGAGC ACTGTTTAAA AGAACCTGAG 
AGGCTGTGCA CAGATAAAGA TCTAATTTTG GATCCCGTTC TTTCAAACAA GCAAGCACAG 
AAACTTCTTC AGCTTATCTG TTATCCTCAT GGTGTGAAAG AATGCTCCGA GGGGGACAAC 
CCTCAGAGGC AGCACATCAA ACGCATACTT CAGAACTTAG ACCAGTGGAC TCTGCGGCAG 
TCTTGGTTGG AGCTGCAACT AATGATCAAG CAGTGCATGA AGGAGCCTGG TTCTGGGTCT 
GTGGCTGAAA TGAACAGCTT GTTGGATAAT ATTGCAAAGG CGACAATAGA GGTGTTTCAG 
CAATCCGCTG ATCTAAACAA TAACTCTTCT AACTCTGGTA TAGGCCTCTT CAATCCAAAC 
TCTGTTGGAA ATGCTGACAC AAGTAATACA AGACAGAATG GTAAAAAGAC ATTCCTAAGT 
TCCTCCGAGT GGCGAGGAGT GTGGCTGGTG GCTCCCTTGA TTGCAAAACT ACCTACCTCA 
GTTCAAGGTA GGGTCTTGAA AGCTGCGGGA GAGGAGCTGG AGAAAGGTCA GCATTTGGGG 
TCATCTTCTA AGAAGGAAAG AGATAGACAG AAGCAAAAGA GCATGTCTCT CTTGAGTCAG 
CAGCCTTTCC TTTCTTTGGT ACTTACTTGC CTTAAGGGAC AGGATGAGCA GCGGGAAGGG 
CTTCTAACAT CACTGCAGAA TCAAGTCAAT CAGATCCTTA GTAACTGGAG GGAAGAACGG 
TACCAGGGTG ATGTTAAAGC TAGGCAAATG ATGCATGAAG CCTTACAGCT TCGTCTGAAT 
TTGGTAGGTG GCATGTTTGA TACTGTGCAG AGGAGTACCC AGTGGACCAC AGACTGGGCA 
CTTCTGCTCC TCCAGATAAT CACTTCGGGA ACAGTTGATA TGCAGACCAA CAACGAATTG 
TTCACAACCG TGCTGGATAT GCTGGGTGTC CTCATCAACG GGACGCTCGC CTCCGACTTG 
TCCAGTGCTT CCCAAGGGGG ACCCGAGGAA AACAAAAGGG CTTACATGAA CTTAGTGAAA 
AAGTTAAAAA AGGAGCTGGG AGACAAGCGG TCAGACAGCA TTGACAAGGT TCGCCAGCTG 
CTCCCTTTGC CAAAGCAGAC ATGTGATATT ATTACCTGTG AGCCAATGGG ATCCTTGATT 
GACACCAAGG GCAACAAAAT TGCGGGGTTT GACTCCATTG ATAAGAAACA GGGTCTTCAG 
GTGTCAACAA AACAAAAGGT GTCCCCTTGG GATTTGTTTG AAGGTCATAA AAATCCTGCT 
CCTCTCTCCT GGGCCTGGTT TGGTACAGTT CGTGTCGACA GGAAGGTGAT CAAATACGAA 
GAGCAGCAGC ATCTGCTCCT GTACCATGCG CACCCCAAAC CCAAGCCGCG GAGCTACTAC 
CTCGAGCCCC TGCCTCTGCC TCCGGAGGAG GAGGAGGAGG AGGAGCCCAC CACCCCTGTC 
TCTCAGGAAC CAGAAAGGAA GTCAGGAGAT CAGGATCAGG GAAAGCATGC CACAGATGAC 
GAGAAGAAGA CAAAGGGCAG GAAGAGGAAG TCAAAGTCAG GCTCTAAGGC TGATGAATAT 
CCACAGAACA ACTTATACAG AGTGCCTCCC AATTACTCTC CCATTCCCTC ACAAATGATG 
CAGCATCCTC AGTCTGCCCT GTGGGGCTAC ACCATCATGG GGCAGCCGCA GCAGCCTGGC 
TTCTTCGTGC AGAACCAGCC CCTCCCTCCA GGTGGTTCCA GGCTGGATCC AACGAGCTCC 
TTCGTCCCAG CCAACACGAA GCAGGCCCTG TCCAACATGC TGCAGCGGCG CTCCGGCACC 
GTGCTGCAGC CCCCTGCCCT CCACGCAGTC ACACCTCAGC AGCAGTTGCT CCAGATGAAA 
CTCCTGCAGC AAGAGCAGCA GCAGCAGCGG CTCCTCAGGC ACCAAGCACA GGCACGGTCT 
CTCCAGCAGG GTCAGCCGAT GGACCCCGCT GCTCTTTTCA CCCCGCAAGT CCGACCCACG 
CCACAGCTAC CGCAGTACCC AGGGCTTCAG CAAGCACAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025009220.1
CDS	33..5954
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPRQKEDELTAVNVKQGFSNQPAFSGDEHGSARNIVINASKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHNWFTDLAGSKPLTILAKKVPILSKKEDVFAYLAKYSVPLLRAAWLIKMTCAYYAAISEAKIKKRQATDPNIEWTQIITRYLREQLAKVAEFYHVTSSQGNSSVVMPQEMEQALKQWEYNEKLSFYMFQEGMLERHEYLTWILDVLEKIRPTDDDILKLVLPLMLQYSEEFVQSAYLSRRLAYFCARRLSLLLSDGPSLAAAHSPHVIIAPSNPPLAVPSPTAPGPVLSPVQLTCSDFLSCPQHRPLVYGLSCMLQTITLCCPSALVWNYSTNENKNMNPGSPLDLLQVAPSSLPMPGGNSTFNQQVRAKIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRSDSSNSMETLYHKIFWANQNKDNQEAAPSDEAVVMLLCEWAVSCKRSGKHRAMVVAKLLEKRQAEIEAERCGEAEVLDEKESLSSASLTSSSLPVFQNILLRFLDTQAPSLSDPNSDHEKTEFVNLVLLFSEFTRHDVFSHDAYMCTLISRGDLSITATTRPRSPNGETGDEHYSKDHDMKMEDHNIMEHMGTDSGPTSIFDDVDKGDFKADFGSEFPIFSPMPGESCENMNSSLDRRISVASEKSVKRERLREVIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRMILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQEAFPTLETVFTKLQQLSYFDQHQVTSQISSNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHACLILNSEQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYSNVQPSNSNLLWDPEFMLDFIENPSAHSINYSMLGKILSDNAANRYSFVCNALMNVCMGHQDAGRINDIANLCAELTACCTVLSSEWLGVVKALCCSSNHVWGFNDLLCSVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDPDAEPGARMTCRLLLHLFRTPQVCLFPQGAGKLFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAILMLGDAEIGSSSVTPVKNEELPVRGLLDEIAEDEMWASSHALKSRGKAVSIETASLSEYARYVLRTICQQEWVGEHCLKEPERLCTDKDLILDPVLSNKQAQKLLQLICYPHGVKECSEGDNPQRQHIKRILQNLDQWTLRQSWLELQLMIKQCMKEPGSGSVAEMNSLLDNIAKATIEVFQQSADLNNNSSNSGIGLFNPNSVGNADTSNTRQNGKKTFLSSSEWRGVWLVAPLIAKLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQGDVKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMQTNNELFTTVLDMLGVLINGTLASDLSSASQGGPEENKRAYMNLVKKLKKELGDKRSDSIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGHKNPAPLSWAWFGTVRVDRKVIKYEEQQHLLLYHAHPKPKPRSYYLEPLPLPPEEEEEEEPTTPVSQEPERKSGDQDQGKHATDDEKKTKGRKRKSKSGSKADEYPQNNLYRVPPNYSPIPSQMMQHPQSALWGYTIMGQPQQPGFFVQNQPLPPGGSRLDPTSSFVPANTKQALSNMLQRRSGTVLQPPALHAVTPQQQLLQMKLLQQEQQQQRLLRHQAQARSLQQGQPMDPAALFTPQVRPTPQLPQYPGLQQAQ

Target ORF information:

RefSeq Version	XM_025153452.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025153452.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAGCTGCTG 
CTGAAGTCGT CCAGCCTTGC AGGGAGCTAC ACCACGGGGC TGTGTGTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCT AAGGTGAAGC AAACGATATA CAGCAATGTT 
CAGCCTTCAA ATTCCAATCT GCTGTGGGAC CCGGAGTTCA TGCTTGATTT TATTGAGAAC 
CCTTCTGCTC ACAGCATTAA CTACTCCATG CTGGGCAAGA TCCTGAGTGA CAACGCAGCC 
AACCGCTACA GCTTTGTCTG CAATGCTCTA ATGAATGTGT GCATGGGGCA CCAAGATGCA 
GGGAGGATTA ATGACATAGC AAACCTTTGT GCAGAGCTGA CGGCGTGCTG CACCGTGCTG 
AGCTCGGAGT GGCTGGGGGT TGTCAAAGCT CTTTGCTGCT CTTCCAATCA CGTGTGGGGT 
TTCAATGATC TGCTCTGCAG CGTGGATGTG AGTGACCTGT CATTCCACGA TTCCCTGGCC 
ACTTTCATTG CCATACTGAT TGCCCGGCAG TGCTTTTCTC TGGAAGATGT CGTTCAGCAC 
GTAGCACTGC CTTCTCTTCT TGCAGCTGCC TGTGGGGACC CAGATGCTGA GCCTGGGGCC 
AGGATGACTT GTCGGCTTCT CCTGCACCTC TTCAGGACAC CCCAGGTCTG CCTCTTTCCC 
CAGGGAGCAG GAAAATTATT TCCTGGAATT CGTTCTTCTT GTGATCGTCA CCTCTTGGCT 
GCTGCTCACA ACAGCATAGA AGTGGGAGCT GTGTTTGCAG TCTTAAAAGC AATTTTGATG 
CTTGGTGACG CTGAGATCGG CAGCAGCAGT GTCACCCCTG TGAAGAACGA GGAGCTGCCC 
GTGAGGGGCT TGCTGGATGA GATCGCTGAG GATGAAATGT GGGCTTCCTC TCACGCTCTG 
AAATCACGTG GAAAAGCTGT TTCCATAGAA ACCGCCAGTT TGAGCGAGTA TGCTAGATAT 
GTGCTAAGGA CCATTTGCCA GCAGGAATGG GTTGGAGAGC ACTGTTTAAA AGAACCTGAG 
AGGCTGTGCA CAGATAAAGA TCTAATTTTG GATCCCGTTC TTTCAAACAA GCAAGCACAG 
AAACTTCTTC AGCTTATCTG TTATCCTCAT GGTGTGAAAG AATGCTCCGA GGGGGACAAC 
CCTCAGAGGC AGCACATCAA ACGCATACTT CAGAACTTAG ACCAGTGGAC TCTGCGGCAG 
TCTTGGTTGG AGCTGCAACT AATGATCAAG CAGTGCATGA AGGAGCCTGG TTCTGGGTCT 
GTGGCTGAAA TGAACAGCTT GTTGGATAAT ATTGCAAAGG CGACAATAGA GGTGTTTCAG 
CAATCCGCTG ATCTAAACAA TAACTCTTCT AACTCTGGTA TAGGCCTCTT CAATCCAAAC 
TCTGTTGGAA ATGCTGACAC AAGTAATACA AGACAGAATG GTAAAAAGAC ATTCCTAAGT 
TCCTCCGAGT GGCGAGGAGT GTGGCTGGTG GCTCCCTTGA TTGCAAAACT ACCTACCTCA 
GTTCAAGGTA GGGTCTTGAA AGCTGCGGGA GAGGAGCTGG AGAAAGGTCA GCATTTGGGG 
TCATCTTCTA AGAAGGAAAG AGATAGACAG AAGCAAAAGA GCATGTCTCT CTTGAGTCAG 
CAGCCTTTCC TTTCTTTGGT ACTTACTTGC CTTAAGGGAC AGGATGAGCA GCGGGAAGGG 
CTTCTAACAT CACTGCAGAA TCAAGTCAAT CAGATCCTTA GTAACTGGAG GGAAGAACGG 
TACCAGGGTG ATGTTAAAGC TAGGCAAATG ATGCATGAAG CCTTACAGCT TCGTCTGAAT 
TTGGTAGGTG GCATGTTTGA TACTGTGCAG AGGAGTACCC AGTGGACCAC AGACTGGGCA 
CTTCTGCTCC TCCAGATAAT CACTTCGGGA ACAGTTGATA TGCAGACCAA CAACGAATTG 
TTCACAACCG TGCTGGATAT GCTGGGTGTC CTCATCAACG GGACGCTCGC CTCCGACTTG 
TCCAGTGCTT CCCAAGGGGG ACCCGAGGAA AACAAAAGGG CTTACATGAA CTTAGTGAAA 
AAGTTAAAAA AGGAGCTGGG AGACAAGCGG TCAGACAGCA TTGACAAGGT TCGCCAGCTG 
CTCCCTTTGC CAAAGCAGAC ATGTGATATT ATTACCTGTG AGCCAATGGG ATCCTTGATT 
GACACCAAGG GCAACAAAAT TGCGGGGTTT GACTCCATTG ATAAGAAACA GGGTCTTCAG 
GTGTCAACAA AACAAAAGGT GTCCCCTTGG GATTTGTTTG AAGGTCATAA AAATCCTGCT 
CCTCTCTCCT GGGCCTGGTT TGGTACAGTT CGTGTCGACA GGAAGGTGAT CAAATACGAA 
GAGCAGCAGC ATCTGCTCCT GTACCATGCG CACCCCAAAC CCAAGCCGCG GAGCTACTAC 
CTCGAGCCCC TGCCTCTGCC TCCGGAGGAG GAGGAGGAGG AGGAGCCCAC CACCCCTGTC 
TCTCAGGAAC CAGAAAGGAA GTCAGGAGAT CAGGATCAGG GAAAGCATGC CACAGATGAC 
GAGAAGAAGA CAAAGGGCAG GAAGAGGAAG TCAAAGTCAG GCTCTAAGGC TGATGAATAT 
CCACAGAACA ACTTATACAG AGTGCCTCCC AATTACTCTC CCATTCCCTC ACAAATGATG 
CAGCATCCTC AGTCTGCCCT GTGGGGCTAC ACCATCATGG GGCAGCCGCA GCAGCCTGGC 
TTCTTCGTGC AGAACCAGCC CCTCCCTCCA GGTGGTTCCA GGCTGGATCC AACGAGCTCC 
TTCGTCCCAG CCAACACGAA GCAGGCCCTG TCCAACATGC TGCAGCGGCG CTCCGGCACC 
GTGCTGCAGC CCCCTGCCCT CCACGCAGTC ACACCTCAGC AGCAGTTGCT CCAGATGAAA 
CTCCTGCAGC AAGAGCAGCA GCAGCAGCGG CTCCTCAGGC ACCAAGCACA GGCACGGTCT 
CTCCAGCAGG GTCAGCCGAT GGACCCCGCT GCTCTTTTCA CCCCGCAAGT CCGACCCACG 
CCACAGCTAC CGCAGTACCC AGGGCTTCAG CAAGCACAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_025153450.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025009218.1 Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X1, mRNA.

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CloneID	OGa152693
Clone ID Related Accession (Same CDS sequence)	XM_025153450.1
Accession Version	XM_025153450.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGAT TTTTTCTCCG 
ATGCCTGGGG AGTCCTGTGA GAACATGAAC TCTTCTTTAG ACAGAAGAAT TTCTGTTGCC 
AGTGAGAAGT CAGTCAAGAG GGAAAGACTC AGAGAAGTGA TTTTTCCATC TAACTATGAC 
CTCCTTCGTC ATCTGCAATA TGCAACGCAT TTCCCAATAC CTCTGGATGA ATCCTCAAGC 
CATGAGTGCA ACCAGCGCAT GATTCTTCTC TATGGTGTTG GCAAGGAGCG TGATGAAGCA 
AGACATCAGC TGAAGAAGAT TACCAAAGAC ATCCTGAAGA TTCTGAATAA GAAGAGTACT 
ACTGAAACGG GTGTTGGGGA TGAAGGGCAA AAAGCCAGGA AGAACAAACA AGAAGCATTT 
CCAACTCTAG AGACTGTGTT CACAAAACTA CAGCAGCTGT CATATTTTGA CCAACATCAG 
GTGACATCTC AGATCTCCAG CAATGTTCTC GAACAAATAA CCAGCTTTGC CTCAGGAACA 
TCCTATCACC TCCCCTTGGC TCACCACATA CAGCTCATCT TTGATCTCAT GGAACCAGCA 
CTGAATATCA ACGGGCTGAT AGACTTTGCA ATACAGCTGC TGAATGAGCT GAGCGTGGTG 
GAGGCAGAGC TGCTGCTGAA GTCGTCCAGC CTTGCAGGGA GCTACACCAC GGGGCTGTGT 
GTGTGCATCG TTGCCGTCCT GCGGCGTTAC CACGCCTGCC TCATTCTCAA CTCGGAGCAA 
ACTGCACAAG TCTTTGAAGG GTTGTGTGGA GTGGTGAAGC ACGTTGTTAA TCCCTCAGAG 
TGTTCCTCCC CAGAAAGATG TATTTTGGCC TACCTCTATG ACCTGTATGT GTCCTGCAGC 
CACCTGCGAA GCAAGTTTGG AGACCTCTTC AGTAGTGCCT GTTCTAAGGT GAAGCAAACG 
ATATACAGCA ATGTTCAGCC TTCAAATTCC AATCTGCTGT GGGACCCGGA GTTCATGCTT 
GATTTTATTG AGAACCCTTC TGCTCACAGC ATTAACTACT CCATGCTGGG CAAGATCCTG 
AGTGACAACG CAGCCAACCG CTACAGCTTT GTCTGCAATG CTCTAATGAA TGTGTGCATG 
GGGCACCAAG ATGCAGGGAG GATTAATGAC ATAGCAAACC TTTGTGCAGA GCTGACGGCG 
TGCTGCACCG TGCTGAGCTC GGAGTGGCTG GGGGTTGTCA AAGCTCTTTG CTGCTCTTCC 
AATCACGTGT GGGGTTTCAA TGATCTGCTC TGCAGCGTGG ATGTGAGTGA CCTGTCATTC 
CACGATTCCC TGGCCACTTT CATTGCCATA CTGATTGCCC GGCAGTGCTT TTCTCTGGAA 
GATGTCGTTC AGCACGTAGC ACTGCCTTCT CTTCTTGCAG CTGCCTGTGG GGACCCAGAT 
GCTGAGCCTG GGGCCAGGAT GACTTGTCGG CTTCTCCTGC ACCTCTTCAG GACACCCCAG 
GTCTGCCTCT TTCCCCAGGG AGCAGGAAAA TTATTTCCTG GAATTCGTTC TTCTTGTGAT 
CGTCACCTCT TGGCTGCTGC TCACAACAGC ATAGAAGTGG GAGCTGTGTT TGCAGTCTTA 
AAAGCAATTT TGATGCTTGG TGACGCTGAG ATCGGCAGCA GCAGTGTCAC CCCTGTGAAG 
AACGAGGAGC TGCCCGTGAG GGGCTTGCTG GATGAGATCG CTGAGGATGA AATGTGGGCT 
TCCTCTCACG CTCTGAAATC ACGTGGAAAA GCTGTTTCCA TAGAAACCGC CAGTTTGAGC 
GAGTATGCTA GATATGTGCT AAGGACCATT TGCCAGCAGG AATGGGTTGG AGAGCACTGT 
TTAAAAGAAC CTGAGAGGCT GTGCACAGAT AAAGATCTAA TTTTGGATCC CGTTCTTTCA 
AACAAGCAAG CACAGAAACT TCTTCAGCTT ATCTGTTATC CTCATGGTGT GAAAGAATGC 
TCCGAGGGGG ACAACCCTCA GAGGCAGCAC ATCAAACGCA TACTTCAGAA CTTAGACCAG 
TGGACTCTGC GGCAGTCTTG GTTGGAGCTG CAACTAATGA TCAAGCAGTG CATGAAGGAG 
CCTGGTTCTG GGTCTGTGGC TGAAATGAAC AGCTTGTTGG ATAATATTGC AAAGGCGACA 
ATAGAGGTGT TTCAGCAATC CGCTGATCTA AACAATAACT CTTCTAACTC TGGTATAGGC 
CTCTTCAATC CAAACTCTGT TGGAAATGCT GACACAAGTA ATACAAGACA GAATGGTAAA 
AAGACATTCC TAAGTTCCTC CGAGTGGCGA GGAGTGTGGC TGGTGGCTCC CTTGATTGCA 
AAACTACCTA CCTCAGTTCA AGGTAGGGTC TTGAAAGCTG CGGGAGAGGA GCTGGAGAAA 
GGTCAGCATT TGGGGTCATC TTCTAAGAAG GAAAGAGATA GACAGAAGCA AAAGAGCATG 
TCTCTCTTGA GTCAGCAGCC TTTCCTTTCT TTGGTACTTA CTTGCCTTAA GGGACAGGAT 
GAGCAGCGGG AAGGGCTTCT AACATCACTG CAGAATCAAG TCAATCAGAT CCTTAGTAAC 
TGGAGGGAAG AACGGTACCA GGGTGATGTT AAAGCTAGGC AAATGATGCA TGAAGCCTTA 
CAGCTTCGTC TGAATTTGGT AGGTGGCATG TTTGATACTG TGCAGAGGAG TACCCAGTGG 
ACCACAGACT GGGCACTTCT GCTCCTCCAG ATAATCACTT CGGGAACAGT TGATATGCAG 
ACCAACAACG AATTGTTCAC AACCGTGCTG GATATGCTGG GTGTCCTCAT CAACGGGACG 
CTCGCCTCCG ACTTGTCCAG TGCTTCCCAA GGGGGACCCG AGGAAAACAA AAGGGCTTAC 
ATGAACTTAG TGAAAAAGTT AAAAAAGGAG CTGGGAGACA AGCGGTCAGA CAGCATTGAC 
AAGGTTCGCC AGCTGCTCCC TTTGCCAAAG CAGACATGTG ATATTATTAC CTGTGAGCCA 
ATGGGATCCT TGATTGACAC CAAGGGCAAC AAAATTGCGG GGTTTGACTC CATTGATAAG 
AAACAGGGTC TTCAGGTGTC AACAAAACAA AAGGTGTCCC CTTGGGATTT GTTTGAAGGT 
CATAAAAATC CTGCTCCTCT CTCCTGGGCC TGGTTTGGTA CAGTTCGTGT CGACAGGAAG 
GTGATCAAAT ACGAAGAGCA GCAGCATCTG CTCCTGTACC ATGCGCACCC CAAACCCAAG 
CCGCGGAGCT ACTACCTCGA GCCCCTGCCT CTGCCTCCGG AGGAGGAGGA GGAGGAGGAG 
CCCACCACCC CTGTCTCTCA GGAACCAGAA AGGAAGTCAG GAGATCAGGA TCAGGGAAAG 
CATGCCACAG ATGACGAGAA GAAGACAAAG GGCAGGAAGA GGAAGTCAAA GTCAGGCTCT 
AAGGCTGATG AATATCCACA GAACAACTTA TACAGAGTGC CTCCCAATTA CTCTCCCATT 
CCCTCACAAA TGATGCAGCA TCCTCAGTCT GCCCTGTGGG GCTACACCAT CATGGGGCAG 
CCGCAGCAGC CTGGCTTCTT CGTGCAGAAC CAGCCCCTCC CTCCAGGTGG TTCCAGGCTG 
GATCCAACGA GCTCCTTCGT CCCAGCCAAC ACGAAGCAGG CCCTGTCCAA CATGCTGCAG 
CGGCGCTCCG GCACCGTGCT GCAGCCCCCT GCCCTCCACG CAGTCACACC TCAGCAGCAG 
TTGCTCCAGA TGAAACTCCT GCAGCAAGAG CAGCAGCAGC AGCGGCTCCT CAGGCACCAA 
GCACAGGCAC GGTCTCTCCA GCAGCAGTTT CCTGTTGTTT CACCAGTGGC TTTCCTGATG 
GGATGGCACG GTGCTGTGTA CCACCACGCT ACCAGTCAGC AGCAAGTACT CCTTACTTAC 
AAAAATTCCA GGGAGTCCAA CAGAAGCTGT TTCCATACAC ATGCACACGG GAGTGCATGT 
TCTCCAGAAG CAGCTGGGTC AGCCGATGGA CCCCGCTGCT CTTTTCACCC CGCAAGTCCG 
ACCCACGCCA CAGCTACCGC AGTACCCAGG GCTTCAGCAA GCACAGGTAC TGCACTGCTC 
CACAGAAGCA TGCCCCACGG GTACACGATG TACAGCACCC CGATGCCTTT GCAGCAGCAG 
CAGCAGCCCA GCAGCGTGGT GCTCTCTCCT GGCTACACCC CGCGGGCATA CACAGCCCAC 
TCCAGCCCCG TGCTGATGGA GCGGCTGCGG CCGGGGCAGC CGGGCAGCGT GGCTCAGCAG 
CAGGCAGCTG CCTACATGCA GCCCCTGGCG GGTGCTCAGC GGTTGACCCA TCAGCCTCTG 
CAGCCAAACT CATTGATAGG GGCAGGCCTG GACAGTGCAT CAACCGCACG TACACATCCA 
ACCTTGAGCT CAGCACAGCT GCCCCAGGAG CAAATGCGAC AGAGGCAGCA GGAGATCCGA 
CAGCAGAGAC TCTTCCAGAT GCAGCAGGGG CAGCAGCAGC AGACTCTTGA TCTTCAGCCT 
GTGCAGCCAC AGCAGCCCCT GGTAAGTGGG ATGGATGGTG AGCGTGGGGC AGGGGCGTGG 
GATGAGCGGG TGAAGCTTAC AGTGAATTTG CAGTGCTGTT GTCCATGTTT CCTGGACATG 
GCCATTTTTT CCAGATATGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025009218.1
CDS	33..6596
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPRQKEDELTAVNVKQGFSNQPAFSGDEHGSARNIVINASKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHNWFTDLAGSKPLTILAKKVPILSKKEDVFAYLAKYSVPLLRAAWLIKMTCAYYAAISEAKIKKRQATDPNIEWTQIITRYLREQLAKVAEFYHVTSSQGNSSVVMPQEMEQALKQWEYNEKLSFYMFQEGMLERHEYLTWILDVLEKIRPTDDDILKLVLPLMLQYSEEFVQSAYLSRRLAYFCARRLSLLLSDGPSLAAAHSPHVIIAPSNPPLAVPSPTAPGPVLSPVQLTCSDFLSCPQHRPLVYGLSCMLQTITLCCPSALVWNYSTNENKNMNPGSPLDLLQVAPSSLPMPGGNSTFNQQVRAKIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRSDSSNSMETLYHKIFWANQNKDNQEAAPSDEAVVMLLCEWAVSCKRSGKHRAMVVAKLLEKRQAEIEAERCGEAEVLDEKESLSSASLTSSSLPVFQNILLRFLDTQAPSLSDPNSDHEKTEFVNLVLLFSEFTRHDVFSHDAYMCTLISRGDLSITATTRPRSPNGETGDEHYSKDHDMKMEIFSPMPGESCENMNSSLDRRISVASEKSVKRERLREVIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRMILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQEAFPTLETVFTKLQQLSYFDQHQVTSQISSNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHACLILNSEQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYSNVQPSNSNLLWDPEFMLDFIENPSAHSINYSMLGKILSDNAANRYSFVCNALMNVCMGHQDAGRINDIANLCAELTACCTVLSSEWLGVVKALCCSSNHVWGFNDLLCSVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDPDAEPGARMTCRLLLHLFRTPQVCLFPQGAGKLFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAILMLGDAEIGSSSVTPVKNEELPVRGLLDEIAEDEMWASSHALKSRGKAVSIETASLSEYARYVLRTICQQEWVGEHCLKEPERLCTDKDLILDPVLSNKQAQKLLQLICYPHGVKECSEGDNPQRQHIKRILQNLDQWTLRQSWLELQLMIKQCMKEPGSGSVAEMNSLLDNIAKATIEVFQQSADLNNNSSNSGIGLFNPNSVGNADTSNTRQNGKKTFLSSSEWRGVWLVAPLIAKLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQGDVKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMQTNNELFTTVLDMLGVLINGTLASDLSSASQGGPEENKRAYMNLVKKLKKELGDKRSDSIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGHKNPAPLSWAWFGTVRVDRKVIKYEEQQHLLLYHAHPKPKPRSYYLEPLPLPPEEEEEEEPTTPVSQEPERKSGDQDQGKHATDDEKKTKGRKRKSKSGSKADEYPQNNLYRVPPNYSPIPSQMMQHPQSALWGYTIMGQPQQPGFFVQNQPLPPGGSRLDPTSSFVPANTKQALSNMLQRRSGTVLQPPALHAVTPQQQLLQMKLLQQEQQQQRLLRHQAQARSLQQQFPVVSPVAFLMGWHGAVYHHATSQQQVLLTYKNSRESNRSCFHTHAHGSACSPEAAGSADGPRCSFHPASPTHATATAVPRASASTGTALLHRSMPHGYTMYSTPMPLQQQQQPSSVVLSPGYTPRAYTAHSSPVLMERLRPGQPGSVAQQQAAAYMQPLAGAQRLTHQPLQPNSLIGAGLDSASTARTHPTLSSAQLPQEQMRQRQQEIRQQRLFQMQQGQQQQTLDLQPVQPQQPLVSGMDGERGAGAWDERVKLTVNLQCCCPCFLDMAIFSRYV

Target ORF information:

RefSeq Version	XM_025153450.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025153450.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGAT TTTTTCTCCG 
ATGCCTGGGG AGTCCTGTGA GAACATGAAC TCTTCTTTAG ACAGAAGAAT TTCTGTTGCC 
AGTGAGAAGT CAGTCAAGAG GGAAAGACTC AGAGAAGTGA TTTTTCCATC TAACTATGAC 
CTCCTTCGTC ATCTGCAATA TGCAACGCAT TTCCCAATAC CTCTGGATGA ATCCTCAAGC 
CATGAGTGCA ACCAGCGCAT GATTCTTCTC TATGGTGTTG GCAAGGAGCG TGATGAAGCA 
AGACATCAGC TGAAGAAGAT TACCAAAGAC ATCCTGAAGA TTCTGAATAA GAAGAGTACT 
ACTGAAACGG GTGTTGGGGA TGAAGGGCAA AAAGCCAGGA AGAACAAACA AGAAGCATTT 
CCAACTCTAG AGACTGTGTT CACAAAACTA CAGCAGCTGT CATATTTTGA CCAACATCAG 
GTGACATCTC AGATCTCCAG CAATGTTCTC GAACAAATAA CCAGCTTTGC CTCAGGAACA 
TCCTATCACC TCCCCTTGGC TCACCACATA CAGCTCATCT TTGATCTCAT GGAACCAGCA 
CTGAATATCA ACGGGCTGAT AGACTTTGCA ATACAGCTGC TGAATGAGCT GAGCGTGGTG 
GAGGCAGAGC TGCTGCTGAA GTCGTCCAGC CTTGCAGGGA GCTACACCAC GGGGCTGTGT 
GTGTGCATCG TTGCCGTCCT GCGGCGTTAC CACGCCTGCC TCATTCTCAA CTCGGAGCAA 
ACTGCACAAG TCTTTGAAGG GTTGTGTGGA GTGGTGAAGC ACGTTGTTAA TCCCTCAGAG 
TGTTCCTCCC CAGAAAGATG TATTTTGGCC TACCTCTATG ACCTGTATGT GTCCTGCAGC 
CACCTGCGAA GCAAGTTTGG AGACCTCTTC AGTAGTGCCT GTTCTAAGGT GAAGCAAACG 
ATATACAGCA ATGTTCAGCC TTCAAATTCC AATCTGCTGT GGGACCCGGA GTTCATGCTT 
GATTTTATTG AGAACCCTTC TGCTCACAGC ATTAACTACT CCATGCTGGG CAAGATCCTG 
AGTGACAACG CAGCCAACCG CTACAGCTTT GTCTGCAATG CTCTAATGAA TGTGTGCATG 
GGGCACCAAG ATGCAGGGAG GATTAATGAC ATAGCAAACC TTTGTGCAGA GCTGACGGCG 
TGCTGCACCG TGCTGAGCTC GGAGTGGCTG GGGGTTGTCA AAGCTCTTTG CTGCTCTTCC 
AATCACGTGT GGGGTTTCAA TGATCTGCTC TGCAGCGTGG ATGTGAGTGA CCTGTCATTC 
CACGATTCCC TGGCCACTTT CATTGCCATA CTGATTGCCC GGCAGTGCTT TTCTCTGGAA 
GATGTCGTTC AGCACGTAGC ACTGCCTTCT CTTCTTGCAG CTGCCTGTGG GGACCCAGAT 
GCTGAGCCTG GGGCCAGGAT GACTTGTCGG CTTCTCCTGC ACCTCTTCAG GACACCCCAG 
GTCTGCCTCT TTCCCCAGGG AGCAGGAAAA TTATTTCCTG GAATTCGTTC TTCTTGTGAT 
CGTCACCTCT TGGCTGCTGC TCACAACAGC ATAGAAGTGG GAGCTGTGTT TGCAGTCTTA 
AAAGCAATTT TGATGCTTGG TGACGCTGAG ATCGGCAGCA GCAGTGTCAC CCCTGTGAAG 
AACGAGGAGC TGCCCGTGAG GGGCTTGCTG GATGAGATCG CTGAGGATGA AATGTGGGCT 
TCCTCTCACG CTCTGAAATC ACGTGGAAAA GCTGTTTCCA TAGAAACCGC CAGTTTGAGC 
GAGTATGCTA GATATGTGCT AAGGACCATT TGCCAGCAGG AATGGGTTGG AGAGCACTGT 
TTAAAAGAAC CTGAGAGGCT GTGCACAGAT AAAGATCTAA TTTTGGATCC CGTTCTTTCA 
AACAAGCAAG CACAGAAACT TCTTCAGCTT ATCTGTTATC CTCATGGTGT GAAAGAATGC 
TCCGAGGGGG ACAACCCTCA GAGGCAGCAC ATCAAACGCA TACTTCAGAA CTTAGACCAG 
TGGACTCTGC GGCAGTCTTG GTTGGAGCTG CAACTAATGA TCAAGCAGTG CATGAAGGAG 
CCTGGTTCTG GGTCTGTGGC TGAAATGAAC AGCTTGTTGG ATAATATTGC AAAGGCGACA 
ATAGAGGTGT TTCAGCAATC CGCTGATCTA AACAATAACT CTTCTAACTC TGGTATAGGC 
CTCTTCAATC CAAACTCTGT TGGAAATGCT GACACAAGTA ATACAAGACA GAATGGTAAA 
AAGACATTCC TAAGTTCCTC CGAGTGGCGA GGAGTGTGGC TGGTGGCTCC CTTGATTGCA 
AAACTACCTA CCTCAGTTCA AGGTAGGGTC TTGAAAGCTG CGGGAGAGGA GCTGGAGAAA 
GGTCAGCATT TGGGGTCATC TTCTAAGAAG GAAAGAGATA GACAGAAGCA AAAGAGCATG 
TCTCTCTTGA GTCAGCAGCC TTTCCTTTCT TTGGTACTTA CTTGCCTTAA GGGACAGGAT 
GAGCAGCGGG AAGGGCTTCT AACATCACTG CAGAATCAAG TCAATCAGAT CCTTAGTAAC 
TGGAGGGAAG AACGGTACCA GGGTGATGTT AAAGCTAGGC AAATGATGCA TGAAGCCTTA 
CAGCTTCGTC TGAATTTGGT AGGTGGCATG TTTGATACTG TGCAGAGGAG TACCCAGTGG 
ACCACAGACT GGGCACTTCT GCTCCTCCAG ATAATCACTT CGGGAACAGT TGATATGCAG 
ACCAACAACG AATTGTTCAC AACCGTGCTG GATATGCTGG GTGTCCTCAT CAACGGGACG 
CTCGCCTCCG ACTTGTCCAG TGCTTCCCAA GGGGGACCCG AGGAAAACAA AAGGGCTTAC 
ATGAACTTAG TGAAAAAGTT AAAAAAGGAG CTGGGAGACA AGCGGTCAGA CAGCATTGAC 
AAGGTTCGCC AGCTGCTCCC TTTGCCAAAG CAGACATGTG ATATTATTAC CTGTGAGCCA 
ATGGGATCCT TGATTGACAC CAAGGGCAAC AAAATTGCGG GGTTTGACTC CATTGATAAG 
AAACAGGGTC TTCAGGTGTC AACAAAACAA AAGGTGTCCC CTTGGGATTT GTTTGAAGGT 
CATAAAAATC CTGCTCCTCT CTCCTGGGCC TGGTTTGGTA CAGTTCGTGT CGACAGGAAG 
GTGATCAAAT ACGAAGAGCA GCAGCATCTG CTCCTGTACC ATGCGCACCC CAAACCCAAG 
CCGCGGAGCT ACTACCTCGA GCCCCTGCCT CTGCCTCCGG AGGAGGAGGA GGAGGAGGAG 
CCCACCACCC CTGTCTCTCA GGAACCAGAA AGGAAGTCAG GAGATCAGGA TCAGGGAAAG 
CATGCCACAG ATGACGAGAA GAAGACAAAG GGCAGGAAGA GGAAGTCAAA GTCAGGCTCT 
AAGGCTGATG AATATCCACA GAACAACTTA TACAGAGTGC CTCCCAATTA CTCTCCCATT 
CCCTCACAAA TGATGCAGCA TCCTCAGTCT GCCCTGTGGG GCTACACCAT CATGGGGCAG 
CCGCAGCAGC CTGGCTTCTT CGTGCAGAAC CAGCCCCTCC CTCCAGGTGG TTCCAGGCTG 
GATCCAACGA GCTCCTTCGT CCCAGCCAAC ACGAAGCAGG CCCTGTCCAA CATGCTGCAG 
CGGCGCTCCG GCACCGTGCT GCAGCCCCCT GCCCTCCACG CAGTCACACC TCAGCAGCAG 
TTGCTCCAGA TGAAACTCCT GCAGCAAGAG CAGCAGCAGC AGCGGCTCCT CAGGCACCAA 
GCACAGGCAC GGTCTCTCCA GCAGCAGTTT CCTGTTGTTT CACCAGTGGC TTTCCTGATG 
GGATGGCACG GTGCTGTGTA CCACCACGCT ACCAGTCAGC AGCAAGTACT CCTTACTTAC 
AAAAATTCCA GGGAGTCCAA CAGAAGCTGT TTCCATACAC ATGCACACGG GAGTGCATGT 
TCTCCAGAAG CAGCTGGGTC AGCCGATGGA CCCCGCTGCT CTTTTCACCC CGCAAGTCCG 
ACCCACGCCA CAGCTACCGC AGTACCCAGG GCTTCAGCAA GCACAGGTAC TGCACTGCTC 
CACAGAAGCA TGCCCCACGG GTACACGATG TACAGCACCC CGATGCCTTT GCAGCAGCAG 
CAGCAGCCCA GCAGCGTGGT GCTCTCTCCT GGCTACACCC CGCGGGCATA CACAGCCCAC 
TCCAGCCCCG TGCTGATGGA GCGGCTGCGG CCGGGGCAGC CGGGCAGCGT GGCTCAGCAG 
CAGGCAGCTG CCTACATGCA GCCCCTGGCG GGTGCTCAGC GGTTGACCCA TCAGCCTCTG 
CAGCCAAACT CATTGATAGG GGCAGGCCTG GACAGTGCAT CAACCGCACG TACACATCCA 
ACCTTGAGCT CAGCACAGCT GCCCCAGGAG CAAATGCGAC AGAGGCAGCA GGAGATCCGA 
CAGCAGAGAC TCTTCCAGAT GCAGCAGGGG CAGCAGCAGC AGACTCTTGA TCTTCAGCCT 
GTGCAGCCAC AGCAGCCCCT GGTAAGTGGG ATGGATGGTG AGCGTGGGGC AGGGGCGTGG 
GATGAGCGGG TGAAGCTTAC AGTGAATTTG CAGTGCTGTT GTCCATGTTT CCTGGACATG 
GCCATTTTTT CCAGATATGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_015291828.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147314.2 Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X2, mRNA.

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CloneID	OGa152694
Clone ID Related Accession (Same CDS sequence)	XM_015291828.2
Accession Version	XM_015291828.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291828.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAGCTGCTG 
CTGAAGTCGT CCAGCCTTGC AGGGAGCTAC ACCACGGGGC TGTGTGTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCT AAGGTGAAGC AAACGATATA CAGCAATGTT 
CAGCCTTCAA ATTCCAATCT GCTGTGGGAC CCGGAGTTCA TGCTTGATTT TATTGAGAAC 
CCTTCTGCTC ACAGCATTAA CTACTCCATG CTGGGCAAGA TCCTGAGTGA CAACGCAGCC 
AACCGCTACA GCTTTGTCTG CAATGCTCTA ATGAATGTGT GCATGGGGCA CCAAGATGCA 
GGGAGGATTA ATGACATAGC AAACCTTTGT GCAGAGCTGA CGGCGTGCTG CACCGTGCTG 
AGCTCGGAGT GGCTGGGGGT TGTCAAAGCT CTTTGCTGCT CTTCCAATCA CGTGTGGGGT 
TTCAATGATC TGCTCTGCAG CGTGGATGTG AGTGACCTGT CATTCCACGA TTCCCTGGCC 
ACTTTCATTG CCATACTGAT TGCCCGGCAG TGCTTTTCTC TGGAAGATGT CGTTCAGCAC 
GTAGCACTGC CTTCTCTTCT TGCAGCTGCC TGTGGGGACC CAGATGCTGA GCCTGGGGCC 
AGGATGACTT GTCGGCTTCT CCTGCACCTC TTCAGGACAC CCCAGGTCTG CCTCTTTCCC 
CAGGGAGCAG GAAAATTATT TCCTGGAATT CGTTCTTCTT GTGATCGTCA CCTCTTGGCT 
GCTGCTCACA ACAGCATAGA AGTGGGAGCT GTGTTTGCAG TCTTAAAAGC AATTTTGATG 
CTTGGTGACG CTGAGATCGG CAGCAGCAGT GTCACCCCTG TGAAGAACGA GGAGCTGCCC 
GTGAGGGGCT TGCTGGATGA GATCGCTGAG GATGAAATGT GGGCTTCCTC TCACGCTCTG 
AAATCACGTG GAAAAGCTGT TTCCATAGAA ACCGCCAGTT TGAGCGAGTA TGCTAGATAT 
GTGCTAAGGA CCATTTGCCA GCAGGAATGG GTTGGAGAGC ACTGTTTAAA AGAACCTGAG 
AGGCTGTGCA CAGATAAAGA TCTAATTTTG GATCCCGTTC TTTCAAACAA GCAAGCACAG 
AAACTTCTTC AGCTTATCTG TTATCCTCAT GGTGTGAAAG AATGCTCCGA GGGGGACAAC 
CCTCAGAGGC AGCACATCAA ACGCATACTT CAGAACTTAG ACCAGTGGAC TCTGCGGCAG 
TCTTGGTTGG AGCTGCAACT AATGATCAAG CAGTGCATGA AGGAGCCTGG TTCTGGGTCT 
GTGGCTGAAA TGAACAGCTT GTTGGATAAT ATTGCAAAGG CGACAATAGA GGTGTTTCAG 
CAATCCGCTG ATCTAAACAA TAACTCTTCT AACTCTGGTA TAGGCCTCTT CAATCCAAAC 
TCTGTTGGAA ATGCTGACAC AAGTAATACA AGACAGAATG GTAAAAAGAC ATTCCTAAGT 
TCCTCCGAGT GGCGAGGAGT GTGGCTGGTG GCTCCCTTGA TTGCAAAACT ACCTACCTCA 
GTTCAAGGTA GGGTCTTGAA AGCTGCGGGA GAGGAGCTGG AGAAAGGTCA GCATTTGGGG 
TCATCTTCTA AGAAGGAAAG AGATAGACAG AAGCAAAAGA GCATGTCTCT CTTGAGTCAG 
CAGCCTTTCC TTTCTTTGGT ACTTACTTGC CTTAAGGGAC AGGATGAGCA GCGGGAAGGG 
CTTCTAACAT CACTGCAGAA TCAAGTCAAT CAGATCCTTA GTAACTGGAG GGAAGAACGG 
TACCAGGGTG ATGTTAAAGC TAGGCAAATG ATGCATGAAG CCTTACAGCT TCGTCTGAAT 
TTGGTAGGTG GCATGTTTGA TACTGTGCAG AGGAGTACCC AGTGGACCAC AGACTGGGCA 
CTTCTGCTCC TCCAGATAAT CACTTCGGGA ACAGTTGATA TGCAGACCAA CAACGAATTG 
TTCACAACCG TGCTGGATAT GCTGGGTGTC CTCATCAACG GGACGCTCGC CTCCGACTTG 
TCCAGTGCTT CCCAAGGGGG ACCCGAGGAA AACAAAAGGG CTTACATGAA CTTAGTGAAA 
AAGTTAAAAA AGGAGCTGGG AGACAAGCGG TCAGACAGCA TTGACAAGGT TCGCCAGCTG 
CTCCCTTTGC CAAAGCAGAC ATGTGATATT ATTACCTGTG AGCCAATGGG ATCCTTGATT 
GACACCAAGG GCAACAAAAT TGCGGGGTTT GACTCCATTG ATAAGAAACA GGGTCTTCAG 
GTGTCAACAA AACAAAAGGT GTCCCCTTGG GATTTGTTTG AAGGTCATAA AAATCCTGCT 
CCTCTCTCCT GGGCCTGGTT TGGTACAGTT CGTGTCGACA GGAAGGTGAT CAAATACGAA 
GAGCAGCAGC ATCTGCTCCT GTACCATGCG CACCCCAAAC CCAAGCCGCG GAGCTACTAC 
CTCGAGCCCC TGCCTCTGCC TCCGGAGGAG GAGGAGGAGG AGGAGCCCAC CACCCCTGTC 
TCTCAGGAAC CAGAAAGGAA GTCAGGAGAT CAGGATCAGG GAAAGCATGC CACAGATGAC 
GAGAAGAAGA CAAAGGGCAG GAAGAGGAAG TCAAAGTCAG GCTCTAAGGC TGATGAATAT 
CCACAGAACA ACTTATACAG AGTGCCTCCC AATTACTCTC CCATTCCCTC ACAAATGATG 
CAGCATCCTC AGTCTGCCCT GTGGGGCTAC ACCATCATGG GGCAGCCGCA GCAGCCTGGC 
TTCTTCGTGC AGAACCAGCC CCTCCCTCCA GGTGGTTCCA GGCTGGATCC AACGAGCTCC 
TTCGTCCCAG CCAACACGAA GCAGGCCCTG TCCAACATGC TGCAGCGGCG CTCCGGCACC 
GTGCTGCAGC CCCCTGCCCT CCACGCAGTC ACACCTCAGC AGCAGTTGCT CCAGATGAAA 
CTCCTGCAGC AAGAGCAGCA GCAGCAGCGG CTCCTCAGGC ACCAAGCACA GGCACGGTCT 
CTCCAGCAGG ATGTCAGTAG CTCTGCCGTA TCCCTTCAGG ATGGCTTTGA CAACATAATG 
GGTCAGCCGA TGGACCCCGC TGCTCTTTTC ACCCCGCAAG TCCGACCCAC GCCACAGCTA 
CCGCAGTACC CAGGGCTTCA GCAAGCACAG AGCATGCCCC ACGGGTACAC GATGTACAGC 
ACCCCGATGC CTTTGCAGCA GCAGCAGCAG CCCAGCAGCG TGGTGCTCTC TCCTGGCTAC 
ACCCCGCGGG CATACACAGC CCACTCCAGC CCCGTGCTGA TGGAGCGGCT GCGGCCGGGG 
CAGCCGGGCA GCGTGGCTCA GCAGCAGGCA GCTGCCTACA TGCAGCCCCT GGCGGGTGCT 
CAGCGGTTGA CCCATCAGCC TCTGCAGCCA AACTCATTGA TAGGGGCAGG CCTGGACAGT 
GCATCAACCG CACGTACACA TCCAACCTTG AGCTCAGCAC AGCTGCCCCA GGAGCAAATG 
CGACAGAGGC AGCAGGAGAT CCGACAGCAG AGACTCTTCC AGATGCAGCA GGGGCAGCAG 
CAGCAGACTC TTGATCTTCA GCCTGTGCAG CCACAGCAGC CCCTGGTAAG TGGGATGGAT 
GGTGAGCGTG GGGCAGGGGC GTGGGATGAG CGGGTGAAGC TTACAGTGAA TTTGCAGTGC 
TGTTGTCCAT GTTTCCTGGA CATGGCCATT TTTTCCAGAT ATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147314.2
CDS	33..6560
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPRQKEDELTAVNVKQGFSNQPAFSGDEHGSARNIVINASKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHNWFTDLAGSKPLTILAKKVPILSKKEDVFAYLAKYSVPLLRAAWLIKMTCAYYAAISEAKIKKRQATDPNIEWTQIITRYLREQLAKVAEFYHVTSSQGNSSVVMPQEMEQALKQWEYNEKLSFYMFQEGMLERHEYLTWILDVLEKIRPTDDDILKLVLPLMLQYSEEFVQSAYLSRRLAYFCARRLSLLLSDGPSLAAAHSPHVIIAPSNPPLAVPSPTAPGPVLSPVQLTCSDFLSCPQHRPLVYGLSCMLQTITLCCPSALVWNYSTNENKNMNPGSPLDLLQVAPSSLPMPGGNSTFNQQVRAKIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRSDSSNSMETLYHKIFWANQNKDNQEAAPSDEAVVMLLCEWAVSCKRSGKHRAMVVAKLLEKRQAEIEAERCGEAEVLDEKESLSSASLTSSSLPVFQNILLRFLDTQAPSLSDPNSDHEKTEFVNLVLLFSEFTRHDVFSHDAYMCTLISRGDLSITATTRPRSPNGETGDEHYSKDHDMKMEDHNIMEHMGTDSGPTSIFDDVDKGDFKADFGSEFPIFSPMPGESCENMNSSLDRRISVASEKSVKRERLREVIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRMILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQEAFPTLETVFTKLQQLSYFDQHQVTSQISSNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHACLILNSEQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYSNVQPSNSNLLWDPEFMLDFIENPSAHSINYSMLGKILSDNAANRYSFVCNALMNVCMGHQDAGRINDIANLCAELTACCTVLSSEWLGVVKALCCSSNHVWGFNDLLCSVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDPDAEPGARMTCRLLLHLFRTPQVCLFPQGAGKLFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAILMLGDAEIGSSSVTPVKNEELPVRGLLDEIAEDEMWASSHALKSRGKAVSIETASLSEYARYVLRTICQQEWVGEHCLKEPERLCTDKDLILDPVLSNKQAQKLLQLICYPHGVKECSEGDNPQRQHIKRILQNLDQWTLRQSWLELQLMIKQCMKEPGSGSVAEMNSLLDNIAKATIEVFQQSADLNNNSSNSGIGLFNPNSVGNADTSNTRQNGKKTFLSSSEWRGVWLVAPLIAKLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQGDVKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMQTNNELFTTVLDMLGVLINGTLASDLSSASQGGPEENKRAYMNLVKKLKKELGDKRSDSIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGHKNPAPLSWAWFGTVRVDRKVIKYEEQQHLLLYHAHPKPKPRSYYLEPLPLPPEEEEEEEPTTPVSQEPERKSGDQDQGKHATDDEKKTKGRKRKSKSGSKADEYPQNNLYRVPPNYSPIPSQMMQHPQSALWGYTIMGQPQQPGFFVQNQPLPPGGSRLDPTSSFVPANTKQALSNMLQRRSGTVLQPPALHAVTPQQQLLQMKLLQQEQQQQRLLRHQAQARSLQQDVSSSAVSLQDGFDNIMGQPMDPAALFTPQVRPTPQLPQYPGLQQAQSMPHGYTMYSTPMPLQQQQQPSSVVLSPGYTPRAYTAHSSPVLMERLRPGQPGSVAQQQAAAYMQPLAGAQRLTHQPLQPNSLIGAGLDSASTARTHPTLSSAQLPQEQMRQRQQEIRQQRLFQMQQGQQQQTLDLQPVQPQQPLVSGMDGERGAGAWDERVKLTVNLQCCCPCFLDMAIFSRYV

Target ORF information:

RefSeq Version	XM_015291828.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291828.2

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAGCTGCTG 
CTGAAGTCGT CCAGCCTTGC AGGGAGCTAC ACCACGGGGC TGTGTGTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCT AAGGTGAAGC AAACGATATA CAGCAATGTT 
CAGCCTTCAA ATTCCAATCT GCTGTGGGAC CCGGAGTTCA TGCTTGATTT TATTGAGAAC 
CCTTCTGCTC ACAGCATTAA CTACTCCATG CTGGGCAAGA TCCTGAGTGA CAACGCAGCC 
AACCGCTACA GCTTTGTCTG CAATGCTCTA ATGAATGTGT GCATGGGGCA CCAAGATGCA 
GGGAGGATTA ATGACATAGC AAACCTTTGT GCAGAGCTGA CGGCGTGCTG CACCGTGCTG 
AGCTCGGAGT GGCTGGGGGT TGTCAAAGCT CTTTGCTGCT CTTCCAATCA CGTGTGGGGT 
TTCAATGATC TGCTCTGCAG CGTGGATGTG AGTGACCTGT CATTCCACGA TTCCCTGGCC 
ACTTTCATTG CCATACTGAT TGCCCGGCAG TGCTTTTCTC TGGAAGATGT CGTTCAGCAC 
GTAGCACTGC CTTCTCTTCT TGCAGCTGCC TGTGGGGACC CAGATGCTGA GCCTGGGGCC 
AGGATGACTT GTCGGCTTCT CCTGCACCTC TTCAGGACAC CCCAGGTCTG CCTCTTTCCC 
CAGGGAGCAG GAAAATTATT TCCTGGAATT CGTTCTTCTT GTGATCGTCA CCTCTTGGCT 
GCTGCTCACA ACAGCATAGA AGTGGGAGCT GTGTTTGCAG TCTTAAAAGC AATTTTGATG 
CTTGGTGACG CTGAGATCGG CAGCAGCAGT GTCACCCCTG TGAAGAACGA GGAGCTGCCC 
GTGAGGGGCT TGCTGGATGA GATCGCTGAG GATGAAATGT GGGCTTCCTC TCACGCTCTG 
AAATCACGTG GAAAAGCTGT TTCCATAGAA ACCGCCAGTT TGAGCGAGTA TGCTAGATAT 
GTGCTAAGGA CCATTTGCCA GCAGGAATGG GTTGGAGAGC ACTGTTTAAA AGAACCTGAG 
AGGCTGTGCA CAGATAAAGA TCTAATTTTG GATCCCGTTC TTTCAAACAA GCAAGCACAG 
AAACTTCTTC AGCTTATCTG TTATCCTCAT GGTGTGAAAG AATGCTCCGA GGGGGACAAC 
CCTCAGAGGC AGCACATCAA ACGCATACTT CAGAACTTAG ACCAGTGGAC TCTGCGGCAG 
TCTTGGTTGG AGCTGCAACT AATGATCAAG CAGTGCATGA AGGAGCCTGG TTCTGGGTCT 
GTGGCTGAAA TGAACAGCTT GTTGGATAAT ATTGCAAAGG CGACAATAGA GGTGTTTCAG 
CAATCCGCTG ATCTAAACAA TAACTCTTCT AACTCTGGTA TAGGCCTCTT CAATCCAAAC 
TCTGTTGGAA ATGCTGACAC AAGTAATACA AGACAGAATG GTAAAAAGAC ATTCCTAAGT 
TCCTCCGAGT GGCGAGGAGT GTGGCTGGTG GCTCCCTTGA TTGCAAAACT ACCTACCTCA 
GTTCAAGGTA GGGTCTTGAA AGCTGCGGGA GAGGAGCTGG AGAAAGGTCA GCATTTGGGG 
TCATCTTCTA AGAAGGAAAG AGATAGACAG AAGCAAAAGA GCATGTCTCT CTTGAGTCAG 
CAGCCTTTCC TTTCTTTGGT ACTTACTTGC CTTAAGGGAC AGGATGAGCA GCGGGAAGGG 
CTTCTAACAT CACTGCAGAA TCAAGTCAAT CAGATCCTTA GTAACTGGAG GGAAGAACGG 
TACCAGGGTG ATGTTAAAGC TAGGCAAATG ATGCATGAAG CCTTACAGCT TCGTCTGAAT 
TTGGTAGGTG GCATGTTTGA TACTGTGCAG AGGAGTACCC AGTGGACCAC AGACTGGGCA 
CTTCTGCTCC TCCAGATAAT CACTTCGGGA ACAGTTGATA TGCAGACCAA CAACGAATTG 
TTCACAACCG TGCTGGATAT GCTGGGTGTC CTCATCAACG GGACGCTCGC CTCCGACTTG 
TCCAGTGCTT CCCAAGGGGG ACCCGAGGAA AACAAAAGGG CTTACATGAA CTTAGTGAAA 
AAGTTAAAAA AGGAGCTGGG AGACAAGCGG TCAGACAGCA TTGACAAGGT TCGCCAGCTG 
CTCCCTTTGC CAAAGCAGAC ATGTGATATT ATTACCTGTG AGCCAATGGG ATCCTTGATT 
GACACCAAGG GCAACAAAAT TGCGGGGTTT GACTCCATTG ATAAGAAACA GGGTCTTCAG 
GTGTCAACAA AACAAAAGGT GTCCCCTTGG GATTTGTTTG AAGGTCATAA AAATCCTGCT 
CCTCTCTCCT GGGCCTGGTT TGGTACAGTT CGTGTCGACA GGAAGGTGAT CAAATACGAA 
GAGCAGCAGC ATCTGCTCCT GTACCATGCG CACCCCAAAC CCAAGCCGCG GAGCTACTAC 
CTCGAGCCCC TGCCTCTGCC TCCGGAGGAG GAGGAGGAGG AGGAGCCCAC CACCCCTGTC 
TCTCAGGAAC CAGAAAGGAA GTCAGGAGAT CAGGATCAGG GAAAGCATGC CACAGATGAC 
GAGAAGAAGA CAAAGGGCAG GAAGAGGAAG TCAAAGTCAG GCTCTAAGGC TGATGAATAT 
CCACAGAACA ACTTATACAG AGTGCCTCCC AATTACTCTC CCATTCCCTC ACAAATGATG 
CAGCATCCTC AGTCTGCCCT GTGGGGCTAC ACCATCATGG GGCAGCCGCA GCAGCCTGGC 
TTCTTCGTGC AGAACCAGCC CCTCCCTCCA GGTGGTTCCA GGCTGGATCC AACGAGCTCC 
TTCGTCCCAG CCAACACGAA GCAGGCCCTG TCCAACATGC TGCAGCGGCG CTCCGGCACC 
GTGCTGCAGC CCCCTGCCCT CCACGCAGTC ACACCTCAGC AGCAGTTGCT CCAGATGAAA 
CTCCTGCAGC AAGAGCAGCA GCAGCAGCGG CTCCTCAGGC ACCAAGCACA GGCACGGTCT 
CTCCAGCAGG ATGTCAGTAG CTCTGCCGTA TCCCTTCAGG ATGGCTTTGA CAACATAATG 
GGTCAGCCGA TGGACCCCGC TGCTCTTTTC ACCCCGCAAG TCCGACCCAC GCCACAGCTA 
CCGCAGTACC CAGGGCTTCA GCAAGCACAG AGCATGCCCC ACGGGTACAC GATGTACAGC 
ACCCCGATGC CTTTGCAGCA GCAGCAGCAG CCCAGCAGCG TGGTGCTCTC TCCTGGCTAC 
ACCCCGCGGG CATACACAGC CCACTCCAGC CCCGTGCTGA TGGAGCGGCT GCGGCCGGGG 
CAGCCGGGCA GCGTGGCTCA GCAGCAGGCA GCTGCCTACA TGCAGCCCCT GGCGGGTGCT 
CAGCGGTTGA CCCATCAGCC TCTGCAGCCA AACTCATTGA TAGGGGCAGG CCTGGACAGT 
GCATCAACCG CACGTACACA TCCAACCTTG AGCTCAGCAC AGCTGCCCCA GGAGCAAATG 
CGACAGAGGC AGCAGGAGAT CCGACAGCAG AGACTCTTCC AGATGCAGCA GGGGCAGCAG 
CAGCAGACTC TTGATCTTCA GCCTGTGCAG CCACAGCAGC CCCTGGTAAG TGGGATGGAT 
GGTGAGCGTG GGGCAGGGGC GTGGGATGAG CGGGTGAAGC TTACAGTGAA TTTGCAGTGC 
TGTTGTCCAT GTTTCCTGGA CATGGCCATT TTTTCCAGAT ATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_015291830.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147316.2 Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X3, mRNA.

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CloneID	OGa152695
Clone ID Related Accession (Same CDS sequence)	XM_015291830.2
Accession Version	XM_015291830.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291830.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAGCTGCTG 
CTGAAGTCGT CCAGCCTTGC AGGGAGCTAC ACCACGGGGC TGTGTGTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCT AAGGTGAAGC AAACGATATA CAGCAATGTT 
CAGCCTTCAA ATTCCAATCT GCTGTGGGAC CCGGAGTTCA TGCTTGATTT TATTGAGAAC 
CCTTCTGCTC ACAGCATTAA CTACTCCATG CTGGGCAAGA TCCTGAGTGA CAACGCAGCC 
AACCGCTACA GCTTTGTCTG CAATGCTCTA ATGAATGTGT GCATGGGGCA CCAAGATGCA 
GGGAGGATTA ATGACATAGC AAACCTTTGT GCAGAGCTGA CGGCGTGCTG CACCGTGCTG 
AGCTCGGAGT GGCTGGGGGT TGTCAAAGCT CTTTGCTGCT CTTCCAATCA CGTGTGGGGT 
TTCAATGATC TGCTCTGCAG CGTGGATGTG AGTGACCTGT CATTCCACGA TTCCCTGGCC 
ACTTTCATTG CCATACTGAT TGCCCGGCAG TGCTTTTCTC TGGAAGATGT CGTTCAGCAC 
GTAGCACTGC CTTCTCTTCT TGCAGCTGCC TGTGGGGACC CAGATGCTGA GCCTGGGGCC 
AGGATGACTT GTCGGCTTCT CCTGCACCTC TTCAGGACAC CCCAGGTCTG CCTCTTTCCC 
CAGGGAGCAG GAAAATTATT TCCTGGAATT CGTTCTTCTT GTGATCGTCA CCTCTTGGCT 
GCTGCTCACA ACAGCATAGA AGTGGGAGCT GTGTTTGCAG TCTTAAAAGC AATTTTGATG 
CTTGGTGACG CTGAGATCGG CAGCAGCAGT GTCACCCCTG TGAAGAACGA GGAGCTGCCC 
GTGAGGGGCT TGCTGGATGA GATCGCTGAG GATGAAATGT GGGCTTCCTC TCACGCTCTG 
AAATCACGTG GAAAAGCTGT TTCCATAGAA ACCGCCAGTT TGAGCGAGTA TGCTAGATAT 
GTGCTAAGGA CCATTTGCCA GCAGGAATGG GTTGGAGAGC ACTGTTTAAA AGAACCTGAG 
AGGCTGTGCA CAGATAAAGA TCTAATTTTG GATCCCGTTC TTTCAAACAA GCAAGCACAG 
AAACTTCTTC AGCTTATCTG TTATCCTCAT GGTGTGAAAG AATGCTCCGA GGGGGACAAC 
CCTCAGAGGC AGCACATCAA ACGCATACTT CAGAACTTAG ACCAGTGGAC TCTGCGGCAG 
TCTTGGTTGG AGCTGCAACT AATGATCAAG CAGTGCATGA AGGAGCCTGG TTCTGGGTCT 
GTGGCTGAAA TGAACAGCTT GTTGGATAAT ATTGCAAAGG CGACAATAGA GGTGTTTCAG 
CAATCCGCTG ATCTAAACAA TAACTCTTCT AACTCTGGTA TAGGCCTCTT CAATCCAAAC 
TCTGTTGGAA ATGCTGACAC AAGTAATACA AGACAGAATG GTAAAAAGAC ATTCCTAAGT 
TCCTCCGAGT GGCGAGGAGT GTGGCTGGTG GCTCCCTTGA TTGCAAAACT ACCTACCTCA 
GTTCAAGGTA GGGTCTTGAA AGCTGCGGGA GAGGAGCTGG AGAAAGGTCA GCATTTGGGG 
TCATCTTCTA AGAAGGAAAG AGATAGACAG AAGCAAAAGA GCATGTCTCT CTTGAGTCAG 
CAGCCTTTCC TTTCTTTGGT ACTTACTTGC CTTAAGGGAC AGGATGAGCA GCGGGAAGGG 
CTTCTAACAT CACTGCAGAA TCAAGTCAAT CAGATCCTTA GTAACTGGAG GGAAGAACGG 
TACCAGGGTG ATGTTAAAGC TAGGCAAATG ATGCATGAAG CCTTACAGCT TCGTCTGAAT 
TTGGTAGGTG GCATGTTTGA TACTGTGCAG AGGAGTACCC AGTGGACCAC AGACTGGGCA 
CTTCTGCTCC TCCAGATAAT CACTTCGGGA ACAGTTGATA TGCAGACCAA CAACGAATTG 
TTCACAACCG TGCTGGATAT GCTGGGTGTC CTCATCAACG GGACGCTCGC CTCCGACTTG 
TCCAGTGCTT CCCAAGGGGG ACCCGAGGAA AACAAAAGGG CTTACATGAA CTTAGTGAAA 
AAGTTAAAAA AGGAGCTGGG AGACAAGCGG TCAGACAGCA TTGACAAGGT TCGCCAGCTG 
CTCCCTTTGC CAAAGCAGAC ATGTGATATT ATTACCTGTG AGCCAATGGG ATCCTTGATT 
GACACCAAGG GCAACAAAAT TGCGGGGTTT GACTCCATTG ATAAGAAACA GGGTCTTCAG 
GTGTCAACAA AACAAAAGGT GTCCCCTTGG GATTTGTTTG AAGGTCATAA AAATCCTGCT 
CCTCTCTCCT GGGCCTGGTT TGGTACAGTT CGTGTCGACA GGAAGGTGAT CAAATACGAA 
GAGCAGCAGC ATCTGCTCCT GTACCATGCG CACCCCAAAC CCAAGCCGCG GAGCTACTAC 
CTCGAGCCCC TGCCTCTGCC TCCGGAGGAG GAGGAGGAGG AGGAGCCCAC CACCCCTGTC 
TCTCAGGAAC CAGAAAGGAA GTCAGGAGAT CAGGATCAGG GAAAGCATGC CACAGATGAC 
GAGAAGAAGA CAAAGGGCAG GAAGAGGAAG TCAAAGTCAG GCTCTAAGGC TGATGAATAT 
CCACAGAACA ACTTATACAG AGTGCCTCCC AATTACTCTC CCATTCCCTC ACAAATGATG 
CAGCATCCTC AGTCTGCCCT GTGGGGCTAC ACCATCATGG GGCAGCCGCA GCAGCCTGGC 
TTCTTCGTGC AGAACCAGCC CCTCCCTCCA GGTGGTTCCA GGCTGGATCC AACGAGCTCC 
TTCGTCCCAG CCAACACGAA GCAGGCCCTG TCCAACATGC TGCAGCGGCG CTCCGGCACC 
GTGCTGCAGC CCCCTGCCCT CCACGCAGTC ACACCTCAGC AGCAGTTGCT CCAGATGAAA 
CTCCTGCAGC AAGAGCAGCA GCAGCAGCGG CTCCTCAGGC ACCAAGCACA GGCACGGTCT 
CTCCAGCAGG GTCAGCCGAT GGACCCCGCT GCTCTTTTCA CCCCGCAAGT CCGACCCACG 
CCACAGCTAC CGCAGTACCC AGGGCTTCAG CAAGCACAGA GCATGCCCCA CGGGTACACG 
ATGTACAGCA CCCCGATGCC TTTGCAGCAG CAGCAGCAGC CCAGCAGCGT GGTGCTCTCT 
CCTGGCTACA CCCCGCGGGC ATACACAGCC CACTCCAGCC CCGTGCTGAT GGAGCGGCTG 
CGGCCGGGGC AGCCGGGCAG CGTGGCTCAG CAGCAGGCAG CTGCCTACAT GCAGCCCCTG 
GCGGGTGCTC AGCGGTTGAC CCATCAGCCT CTGCAGCCAA ACTCATTGAT AGGGGCAGGC 
CTGGACAGTG CATCAACCGC ACGTACACAT CCAACCTTGA GCTCAGCACA GCTGCCCCAG 
GAGCAAATGC GACAGAGGCA GCAGGAGATC CGACAGCAGA GACTCTTCCA GATGCAGCAG 
GGGCAGCAGC AGCAGACTCT TGATCTTCAG CCTGTGCAGC CACAGCAGCC CCTGGTAAGT 
GGGATGGATG GTGAGCGTGG GGCAGGGGCG TGGGATGAGC GGGTGAAGCT TACAGTGAAT 
TTGCAGTGCT GTTGTCCATG TTTCCTGGAC ATGGCCATTT TTTCCAGATA TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147316.2
CDS	33..6509
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPRQKEDELTAVNVKQGFSNQPAFSGDEHGSARNIVINASKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHNWFTDLAGSKPLTILAKKVPILSKKEDVFAYLAKYSVPLLRAAWLIKMTCAYYAAISEAKIKKRQATDPNIEWTQIITRYLREQLAKVAEFYHVTSSQGNSSVVMPQEMEQALKQWEYNEKLSFYMFQEGMLERHEYLTWILDVLEKIRPTDDDILKLVLPLMLQYSEEFVQSAYLSRRLAYFCARRLSLLLSDGPSLAAAHSPHVIIAPSNPPLAVPSPTAPGPVLSPVQLTCSDFLSCPQHRPLVYGLSCMLQTITLCCPSALVWNYSTNENKNMNPGSPLDLLQVAPSSLPMPGGNSTFNQQVRAKIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRSDSSNSMETLYHKIFWANQNKDNQEAAPSDEAVVMLLCEWAVSCKRSGKHRAMVVAKLLEKRQAEIEAERCGEAEVLDEKESLSSASLTSSSLPVFQNILLRFLDTQAPSLSDPNSDHEKTEFVNLVLLFSEFTRHDVFSHDAYMCTLISRGDLSITATTRPRSPNGETGDEHYSKDHDMKMEDHNIMEHMGTDSGPTSIFDDVDKGDFKADFGSEFPIFSPMPGESCENMNSSLDRRISVASEKSVKRERLREVIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRMILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQEAFPTLETVFTKLQQLSYFDQHQVTSQISSNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHACLILNSEQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYSNVQPSNSNLLWDPEFMLDFIENPSAHSINYSMLGKILSDNAANRYSFVCNALMNVCMGHQDAGRINDIANLCAELTACCTVLSSEWLGVVKALCCSSNHVWGFNDLLCSVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDPDAEPGARMTCRLLLHLFRTPQVCLFPQGAGKLFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAILMLGDAEIGSSSVTPVKNEELPVRGLLDEIAEDEMWASSHALKSRGKAVSIETASLSEYARYVLRTICQQEWVGEHCLKEPERLCTDKDLILDPVLSNKQAQKLLQLICYPHGVKECSEGDNPQRQHIKRILQNLDQWTLRQSWLELQLMIKQCMKEPGSGSVAEMNSLLDNIAKATIEVFQQSADLNNNSSNSGIGLFNPNSVGNADTSNTRQNGKKTFLSSSEWRGVWLVAPLIAKLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQGDVKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMQTNNELFTTVLDMLGVLINGTLASDLSSASQGGPEENKRAYMNLVKKLKKELGDKRSDSIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGHKNPAPLSWAWFGTVRVDRKVIKYEEQQHLLLYHAHPKPKPRSYYLEPLPLPPEEEEEEEPTTPVSQEPERKSGDQDQGKHATDDEKKTKGRKRKSKSGSKADEYPQNNLYRVPPNYSPIPSQMMQHPQSALWGYTIMGQPQQPGFFVQNQPLPPGGSRLDPTSSFVPANTKQALSNMLQRRSGTVLQPPALHAVTPQQQLLQMKLLQQEQQQQRLLRHQAQARSLQQGQPMDPAALFTPQVRPTPQLPQYPGLQQAQSMPHGYTMYSTPMPLQQQQQPSSVVLSPGYTPRAYTAHSSPVLMERLRPGQPGSVAQQQAAAYMQPLAGAQRLTHQPLQPNSLIGAGLDSASTARTHPTLSSAQLPQEQMRQRQQEIRQQRLFQMQQGQQQQTLDLQPVQPQQPLVSGMDGERGAGAWDERVKLTVNLQCCCPCFLDMAIFSRYV

Target ORF information:

RefSeq Version	XM_015291830.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291830.2

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAGCTGCTG 
CTGAAGTCGT CCAGCCTTGC AGGGAGCTAC ACCACGGGGC TGTGTGTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCT AAGGTGAAGC AAACGATATA CAGCAATGTT 
CAGCCTTCAA ATTCCAATCT GCTGTGGGAC CCGGAGTTCA TGCTTGATTT TATTGAGAAC 
CCTTCTGCTC ACAGCATTAA CTACTCCATG CTGGGCAAGA TCCTGAGTGA CAACGCAGCC 
AACCGCTACA GCTTTGTCTG CAATGCTCTA ATGAATGTGT GCATGGGGCA CCAAGATGCA 
GGGAGGATTA ATGACATAGC AAACCTTTGT GCAGAGCTGA CGGCGTGCTG CACCGTGCTG 
AGCTCGGAGT GGCTGGGGGT TGTCAAAGCT CTTTGCTGCT CTTCCAATCA CGTGTGGGGT 
TTCAATGATC TGCTCTGCAG CGTGGATGTG AGTGACCTGT CATTCCACGA TTCCCTGGCC 
ACTTTCATTG CCATACTGAT TGCCCGGCAG TGCTTTTCTC TGGAAGATGT CGTTCAGCAC 
GTAGCACTGC CTTCTCTTCT TGCAGCTGCC TGTGGGGACC CAGATGCTGA GCCTGGGGCC 
AGGATGACTT GTCGGCTTCT CCTGCACCTC TTCAGGACAC CCCAGGTCTG CCTCTTTCCC 
CAGGGAGCAG GAAAATTATT TCCTGGAATT CGTTCTTCTT GTGATCGTCA CCTCTTGGCT 
GCTGCTCACA ACAGCATAGA AGTGGGAGCT GTGTTTGCAG TCTTAAAAGC AATTTTGATG 
CTTGGTGACG CTGAGATCGG CAGCAGCAGT GTCACCCCTG TGAAGAACGA GGAGCTGCCC 
GTGAGGGGCT TGCTGGATGA GATCGCTGAG GATGAAATGT GGGCTTCCTC TCACGCTCTG 
AAATCACGTG GAAAAGCTGT TTCCATAGAA ACCGCCAGTT TGAGCGAGTA TGCTAGATAT 
GTGCTAAGGA CCATTTGCCA GCAGGAATGG GTTGGAGAGC ACTGTTTAAA AGAACCTGAG 
AGGCTGTGCA CAGATAAAGA TCTAATTTTG GATCCCGTTC TTTCAAACAA GCAAGCACAG 
AAACTTCTTC AGCTTATCTG TTATCCTCAT GGTGTGAAAG AATGCTCCGA GGGGGACAAC 
CCTCAGAGGC AGCACATCAA ACGCATACTT CAGAACTTAG ACCAGTGGAC TCTGCGGCAG 
TCTTGGTTGG AGCTGCAACT AATGATCAAG CAGTGCATGA AGGAGCCTGG TTCTGGGTCT 
GTGGCTGAAA TGAACAGCTT GTTGGATAAT ATTGCAAAGG CGACAATAGA GGTGTTTCAG 
CAATCCGCTG ATCTAAACAA TAACTCTTCT AACTCTGGTA TAGGCCTCTT CAATCCAAAC 
TCTGTTGGAA ATGCTGACAC AAGTAATACA AGACAGAATG GTAAAAAGAC ATTCCTAAGT 
TCCTCCGAGT GGCGAGGAGT GTGGCTGGTG GCTCCCTTGA TTGCAAAACT ACCTACCTCA 
GTTCAAGGTA GGGTCTTGAA AGCTGCGGGA GAGGAGCTGG AGAAAGGTCA GCATTTGGGG 
TCATCTTCTA AGAAGGAAAG AGATAGACAG AAGCAAAAGA GCATGTCTCT CTTGAGTCAG 
CAGCCTTTCC TTTCTTTGGT ACTTACTTGC CTTAAGGGAC AGGATGAGCA GCGGGAAGGG 
CTTCTAACAT CACTGCAGAA TCAAGTCAAT CAGATCCTTA GTAACTGGAG GGAAGAACGG 
TACCAGGGTG ATGTTAAAGC TAGGCAAATG ATGCATGAAG CCTTACAGCT TCGTCTGAAT 
TTGGTAGGTG GCATGTTTGA TACTGTGCAG AGGAGTACCC AGTGGACCAC AGACTGGGCA 
CTTCTGCTCC TCCAGATAAT CACTTCGGGA ACAGTTGATA TGCAGACCAA CAACGAATTG 
TTCACAACCG TGCTGGATAT GCTGGGTGTC CTCATCAACG GGACGCTCGC CTCCGACTTG 
TCCAGTGCTT CCCAAGGGGG ACCCGAGGAA AACAAAAGGG CTTACATGAA CTTAGTGAAA 
AAGTTAAAAA AGGAGCTGGG AGACAAGCGG TCAGACAGCA TTGACAAGGT TCGCCAGCTG 
CTCCCTTTGC CAAAGCAGAC ATGTGATATT ATTACCTGTG AGCCAATGGG ATCCTTGATT 
GACACCAAGG GCAACAAAAT TGCGGGGTTT GACTCCATTG ATAAGAAACA GGGTCTTCAG 
GTGTCAACAA AACAAAAGGT GTCCCCTTGG GATTTGTTTG AAGGTCATAA AAATCCTGCT 
CCTCTCTCCT GGGCCTGGTT TGGTACAGTT CGTGTCGACA GGAAGGTGAT CAAATACGAA 
GAGCAGCAGC ATCTGCTCCT GTACCATGCG CACCCCAAAC CCAAGCCGCG GAGCTACTAC 
CTCGAGCCCC TGCCTCTGCC TCCGGAGGAG GAGGAGGAGG AGGAGCCCAC CACCCCTGTC 
TCTCAGGAAC CAGAAAGGAA GTCAGGAGAT CAGGATCAGG GAAAGCATGC CACAGATGAC 
GAGAAGAAGA CAAAGGGCAG GAAGAGGAAG TCAAAGTCAG GCTCTAAGGC TGATGAATAT 
CCACAGAACA ACTTATACAG AGTGCCTCCC AATTACTCTC CCATTCCCTC ACAAATGATG 
CAGCATCCTC AGTCTGCCCT GTGGGGCTAC ACCATCATGG GGCAGCCGCA GCAGCCTGGC 
TTCTTCGTGC AGAACCAGCC CCTCCCTCCA GGTGGTTCCA GGCTGGATCC AACGAGCTCC 
TTCGTCCCAG CCAACACGAA GCAGGCCCTG TCCAACATGC TGCAGCGGCG CTCCGGCACC 
GTGCTGCAGC CCCCTGCCCT CCACGCAGTC ACACCTCAGC AGCAGTTGCT CCAGATGAAA 
CTCCTGCAGC AAGAGCAGCA GCAGCAGCGG CTCCTCAGGC ACCAAGCACA GGCACGGTCT 
CTCCAGCAGG GTCAGCCGAT GGACCCCGCT GCTCTTTTCA CCCCGCAAGT CCGACCCACG 
CCACAGCTAC CGCAGTACCC AGGGCTTCAG CAAGCACAGA GCATGCCCCA CGGGTACACG 
ATGTACAGCA CCCCGATGCC TTTGCAGCAG CAGCAGCAGC CCAGCAGCGT GGTGCTCTCT 
CCTGGCTACA CCCCGCGGGC ATACACAGCC CACTCCAGCC CCGTGCTGAT GGAGCGGCTG 
CGGCCGGGGC AGCCGGGCAG CGTGGCTCAG CAGCAGGCAG CTGCCTACAT GCAGCCCCTG 
GCGGGTGCTC AGCGGTTGAC CCATCAGCCT CTGCAGCCAA ACTCATTGAT AGGGGCAGGC 
CTGGACAGTG CATCAACCGC ACGTACACAT CCAACCTTGA GCTCAGCACA GCTGCCCCAG 
GAGCAAATGC GACAGAGGCA GCAGGAGATC CGACAGCAGA GACTCTTCCA GATGCAGCAG 
GGGCAGCAGC AGCAGACTCT TGATCTTCAG CCTGTGCAGC CACAGCAGCC CCTGGTAAGT 
GGGATGGATG GTGAGCGTGG GGCAGGGGCG TGGGATGAGC GGGTGAAGCT TACAGTGAAT 
TTGCAGTGCT GTTGTCCATG TTTCCTGGAC ATGGCCATTT TTTCCAGATA TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_015291831.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147317.2 Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X4, mRNA.

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CloneID	OGa152696
Clone ID Related Accession (Same CDS sequence)	XM_015291831.2
Accession Version	XM_015291831.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291831.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAGCTGCTG 
CTGAAGTCGT CCAGCCTTGC AGGGAGCTAC ACCACGGGGC TGTGTGTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCT AAGGTGAAGC AAACGATATA CAGCAATGTT 
CAGCCTTCAA ATTCCAATCT GCTGTGGGAC CCGGAGTTCA TGCTTGATTT TATTGAGAAC 
CCTTCTGCTC ACAGCATTAA CTACTCCATG CTGGGCAAGA TCCTGAGTGA CAACGCAGCC 
AACCGCTACA GCTTTGTCTG CAATGCTCTA ATGAATGTGT GCATGGGGCA CCAAGATGCA 
GGGAGGATTA ATGACATAGC AAACCTTTGT GCAGAGCTGA CGGCGTGCTG CACCGTGCTG 
AGCTCGGAGT GGCTGGGGGT TGTCAAAGCT CTTTGCTGCT CTTCCAATCA CGTGTGGGGT 
TTCAATGATC TGCTCTGCAG CGTGGATGTG AGTGACCTGT CATTCCACGA TTCCCTGGCC 
ACTTTCATTG CCATACTGAT TGCCCGGCAG TGCTTTTCTC TGGAAGATGT CGTTCAGCAC 
GTAGCACTGC CTTCTCTTCT TGCAGCTGCC TGTGGGGACC CAGATGCTGA GCCTGGGGCC 
AGGATGACTT GTCGGCTTCT CCTGCACCTC TTCAGGACAC CCCAGGTCTG CCTCTTTCCC 
CAGGGAGCAG GAAAATTATT TCCTGGAATT CGTTCTTCTT GTGATCGTCA CCTCTTGGCT 
GCTGCTCACA ACAGCATAGA AGTGGGAGCT GTGTTTGCAG TCTTAAAAGC AATTTTGATG 
CTTGGTGACG CTGAGATCGG CAGCAGCAGT GTCACCCCTG TGAAGAACGA GGAGCTGCCC 
GTGAGGGGCT TGCTGGATGA GATCGCTGAG GATGAAATGT GGGCTTCCTC TCACGCTCTG 
AAATCACGTG GAAAAGCTGT TTCCATAGAA ACCGCCAGTT TGAGCGAGTA TGCTAGATAT 
GTGCTAAGGA CCATTTGCCA GCAGGAATGG GTTGGAGAGC ACTGTTTAAA AGAACCTGAG 
AGGCTGTGCA CAGATAAAGA TCTAATTTTG GATCCCGTTC TTTCAAACAA GCAAGCACAG 
AAACTTCTTC AGCTTATCTG TTATCCTCAT GGTGTGAAAG AATGCTCCGA GGGGGACAAC 
CCTCAGAGGC AGCACATCAA ACGCATACTT CAGAACTTAG ACCAGTGGAC TCTGCGGCAG 
TCTTGGTTGG AGCTGCAACT AATGATCAAG CAGTGCATGA AGGAGCCTGG TTCTGGGTCT 
GTGGCTGAAA TGAACAGCTT GTTGGATAAT ATTGCAAAGG CGACAATAGA GGTGTTTCAG 
CAATCCGCTG ATCTAAACAA TAACTCTTCT AACTCTGGTA TAGGCCTCTT CAATCCAAAC 
TCTGTTGGAA ATGCTGACAC AAGTAATACA AGACAGAATG GTAAAAAGAC ATTCCTAAGT 
TCCTCCGAGT GGCGAGGAGT GTGGCTGGTG GCTCCCTTGA TTGCAAAACT ACCTACCTCA 
GTTCAAGGTA GGGTCTTGAA AGCTGCGGGA GAGGAGCTGG AGAAAGGTCA GCATTTGGGG 
TCATCTTCTA AGAAGGAAAG AGATAGACAG AAGCAAAAGA GCATGTCTCT CTTGAGTCAG 
CAGCCTTTCC TTTCTTTGGT ACTTACTTGC CTTAAGGGAC AGGATGAGCA GCGGGAAGGG 
CTTCTAACAT CACTGCAGAA TCAAGTCAAT CAGATCCTTA GTAACTGGAG GGAAGAACGG 
TACCAGGGTG ATGTTAAAGC TAGGCAAATG ATGCATGAAG CCTTACAGCT TCGTCTGAAT 
TTGGTAGGTG GCATGTTTGA TACTGTGCAG AGGAGTACCC AGTGGACCAC AGACTGGGCA 
CTTCTGCTCC TCCAGATAAT CACTTCGGGA ACAGTTGATA TGCAGACCAA CAACGAATTG 
TTCACAACCG TGCTGGATAT GCTGGGTGTC CTCATCAACG GGACGCTCGC CTCCGACTTG 
TCCAGTGCTT CCCAAGGGGG ACCCGAGGAA AACAAAAGGG CTTACATGAA CTTAGTGAAA 
AAGTTAAAAA AGGAGCTGGG AGACAAGCGG TCAGACAGCA TTGACAAGGT TCGCCAGCTG 
CTCCCTTTGC CAAAGCAGAC ATGTGATATT ATTACCTGTG AGCCAATGGG ATCCTTGATT 
GACACCAAGG GCAACAAAAT TGCGGGGTTT GACTCCATTG ATAAGAAACA GGGTCTTCAG 
GTGTCAACAA AACAAAAGGT GTCCCCTTGG GATTTGTTTG AAGGTCATAA AAATCCTGCT 
CCTCTCTCCT GGGCCTGGTT TGGTACAGTT CGTGTCGACA GGAAGGTGAT CAAATACGAA 
GAGCAGCAGC ATCTGCTCCT GTACCATGCG CACCCCAAAC CCAAGCCGCG GAGCTACTAC 
CTCGAGCCCC TGCCTCTGCC TCCGGAGGAG GAGGAGGAGG AGGAGCCCAC CACCCCTGTC 
TCTCAGGAAC CAGAAAGGAA GTCAGGAGAT CAGGATCAGG GAAAGCATGC CACAGATGAC 
GAGAAGAAGA CAAAGGGCAG GAAGAGGAAG TCAAAGTCAG GCTCTAAGGC TGATGAATAT 
CCACAGAACA ACTTATACAG AGTGCCTCCC AATTACTCTC CCATTCCCTC ACAAATGATG 
CAGCATCCTC AGTCTGCCCT GTGGGGCTAC ACCATCATGG GGCAGCCGCA GCAGCCTGGC 
TTCTTCGTGC AGAACCAGCC CCTCCCTCCA GGTGGTTCCA GGCTGGATCC AACGAGCTCC 
TTCGTCCCAG CCAACACGAA GCAGGCCCTG TCCAACATGC TGCAGCGGCG CTCCGGCACC 
GTGCTGCAGC CCCCTGCCCT CCACGCAGTC ACACCTCAGC AGCAGTTGCT CCAGATGAAA 
CTCCTGCAGC AAGAGCAGCA GCAGCAGCGG CTCCTCAGGC ACCAAGCACA GGGTCAGCCG 
ATGGACCCCG CTGCTCTTTT CACCCCGCAA GTCCGACCCA CGCCACAGCT ACCGCAGTAC 
CCAGGGCTTC AGCAAGCACA GAGCATGCCC CACGGGTACA CGATGTACAG CACCCCGATG 
CCTTTGCAGC AGCAGCAGCA GCCCAGCAGC GTGGTGCTCT CTCCTGGCTA CACCCCGCGG 
GCATACACAG CCCACTCCAG CCCCGTGCTG ATGGAGCGGC TGCGGCCGGG GCAGCCGGGC 
AGCGTGGCTC AGCAGCAGGC AGCTGCCTAC ATGCAGCCCC TGGCGGGTGC TCAGCGGTTG 
ACCCATCAGC CTCTGCAGCC AAACTCATTG ATAGGGGCAG GCCTGGACAG TGCATCAACC 
GCACGTACAC ATCCAACCTT GAGCTCAGCA CAGCTGCCCC AGGAGCAAAT GCGACAGAGG 
CAGCAGGAGA TCCGACAGCA GAGACTCTTC CAGATGCAGC AGGGGCAGCA GCAGCAGACT 
CTTGATCTTC AGCCTGTGCA GCCACAGCAG CCCCTGGTAA GTGGGATGGA TGGTGAGCGT 
GGGGCAGGGG CGTGGGATGA GCGGGTGAAG CTTACAGTGA ATTTGCAGTG CTGTTGTCCA 
TGTTTCCTGG ACATGGCCAT TTTTTCCAGA TATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147317.2
CDS	33..6491
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPRQKEDELTAVNVKQGFSNQPAFSGDEHGSARNIVINASKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHNWFTDLAGSKPLTILAKKVPILSKKEDVFAYLAKYSVPLLRAAWLIKMTCAYYAAISEAKIKKRQATDPNIEWTQIITRYLREQLAKVAEFYHVTSSQGNSSVVMPQEMEQALKQWEYNEKLSFYMFQEGMLERHEYLTWILDVLEKIRPTDDDILKLVLPLMLQYSEEFVQSAYLSRRLAYFCARRLSLLLSDGPSLAAAHSPHVIIAPSNPPLAVPSPTAPGPVLSPVQLTCSDFLSCPQHRPLVYGLSCMLQTITLCCPSALVWNYSTNENKNMNPGSPLDLLQVAPSSLPMPGGNSTFNQQVRAKIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRSDSSNSMETLYHKIFWANQNKDNQEAAPSDEAVVMLLCEWAVSCKRSGKHRAMVVAKLLEKRQAEIEAERCGEAEVLDEKESLSSASLTSSSLPVFQNILLRFLDTQAPSLSDPNSDHEKTEFVNLVLLFSEFTRHDVFSHDAYMCTLISRGDLSITATTRPRSPNGETGDEHYSKDHDMKMEDHNIMEHMGTDSGPTSIFDDVDKGDFKADFGSEFPIFSPMPGESCENMNSSLDRRISVASEKSVKRERLREVIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRMILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQEAFPTLETVFTKLQQLSYFDQHQVTSQISSNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHACLILNSEQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYSNVQPSNSNLLWDPEFMLDFIENPSAHSINYSMLGKILSDNAANRYSFVCNALMNVCMGHQDAGRINDIANLCAELTACCTVLSSEWLGVVKALCCSSNHVWGFNDLLCSVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDPDAEPGARMTCRLLLHLFRTPQVCLFPQGAGKLFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAILMLGDAEIGSSSVTPVKNEELPVRGLLDEIAEDEMWASSHALKSRGKAVSIETASLSEYARYVLRTICQQEWVGEHCLKEPERLCTDKDLILDPVLSNKQAQKLLQLICYPHGVKECSEGDNPQRQHIKRILQNLDQWTLRQSWLELQLMIKQCMKEPGSGSVAEMNSLLDNIAKATIEVFQQSADLNNNSSNSGIGLFNPNSVGNADTSNTRQNGKKTFLSSSEWRGVWLVAPLIAKLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQGDVKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMQTNNELFTTVLDMLGVLINGTLASDLSSASQGGPEENKRAYMNLVKKLKKELGDKRSDSIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGHKNPAPLSWAWFGTVRVDRKVIKYEEQQHLLLYHAHPKPKPRSYYLEPLPLPPEEEEEEEPTTPVSQEPERKSGDQDQGKHATDDEKKTKGRKRKSKSGSKADEYPQNNLYRVPPNYSPIPSQMMQHPQSALWGYTIMGQPQQPGFFVQNQPLPPGGSRLDPTSSFVPANTKQALSNMLQRRSGTVLQPPALHAVTPQQQLLQMKLLQQEQQQQRLLRHQAQGQPMDPAALFTPQVRPTPQLPQYPGLQQAQSMPHGYTMYSTPMPLQQQQQPSSVVLSPGYTPRAYTAHSSPVLMERLRPGQPGSVAQQQAAAYMQPLAGAQRLTHQPLQPNSLIGAGLDSASTARTHPTLSSAQLPQEQMRQRQQEIRQQRLFQMQQGQQQQTLDLQPVQPQQPLVSGMDGERGAGAWDERVKLTVNLQCCCPCFLDMAIFSRYV

Target ORF information:

RefSeq Version	XM_015291831.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291831.2

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAGCTGCTG 
CTGAAGTCGT CCAGCCTTGC AGGGAGCTAC ACCACGGGGC TGTGTGTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCT AAGGTGAAGC AAACGATATA CAGCAATGTT 
CAGCCTTCAA ATTCCAATCT GCTGTGGGAC CCGGAGTTCA TGCTTGATTT TATTGAGAAC 
CCTTCTGCTC ACAGCATTAA CTACTCCATG CTGGGCAAGA TCCTGAGTGA CAACGCAGCC 
AACCGCTACA GCTTTGTCTG CAATGCTCTA ATGAATGTGT GCATGGGGCA CCAAGATGCA 
GGGAGGATTA ATGACATAGC AAACCTTTGT GCAGAGCTGA CGGCGTGCTG CACCGTGCTG 
AGCTCGGAGT GGCTGGGGGT TGTCAAAGCT CTTTGCTGCT CTTCCAATCA CGTGTGGGGT 
TTCAATGATC TGCTCTGCAG CGTGGATGTG AGTGACCTGT CATTCCACGA TTCCCTGGCC 
ACTTTCATTG CCATACTGAT TGCCCGGCAG TGCTTTTCTC TGGAAGATGT CGTTCAGCAC 
GTAGCACTGC CTTCTCTTCT TGCAGCTGCC TGTGGGGACC CAGATGCTGA GCCTGGGGCC 
AGGATGACTT GTCGGCTTCT CCTGCACCTC TTCAGGACAC CCCAGGTCTG CCTCTTTCCC 
CAGGGAGCAG GAAAATTATT TCCTGGAATT CGTTCTTCTT GTGATCGTCA CCTCTTGGCT 
GCTGCTCACA ACAGCATAGA AGTGGGAGCT GTGTTTGCAG TCTTAAAAGC AATTTTGATG 
CTTGGTGACG CTGAGATCGG CAGCAGCAGT GTCACCCCTG TGAAGAACGA GGAGCTGCCC 
GTGAGGGGCT TGCTGGATGA GATCGCTGAG GATGAAATGT GGGCTTCCTC TCACGCTCTG 
AAATCACGTG GAAAAGCTGT TTCCATAGAA ACCGCCAGTT TGAGCGAGTA TGCTAGATAT 
GTGCTAAGGA CCATTTGCCA GCAGGAATGG GTTGGAGAGC ACTGTTTAAA AGAACCTGAG 
AGGCTGTGCA CAGATAAAGA TCTAATTTTG GATCCCGTTC TTTCAAACAA GCAAGCACAG 
AAACTTCTTC AGCTTATCTG TTATCCTCAT GGTGTGAAAG AATGCTCCGA GGGGGACAAC 
CCTCAGAGGC AGCACATCAA ACGCATACTT CAGAACTTAG ACCAGTGGAC TCTGCGGCAG 
TCTTGGTTGG AGCTGCAACT AATGATCAAG CAGTGCATGA AGGAGCCTGG TTCTGGGTCT 
GTGGCTGAAA TGAACAGCTT GTTGGATAAT ATTGCAAAGG CGACAATAGA GGTGTTTCAG 
CAATCCGCTG ATCTAAACAA TAACTCTTCT AACTCTGGTA TAGGCCTCTT CAATCCAAAC 
TCTGTTGGAA ATGCTGACAC AAGTAATACA AGACAGAATG GTAAAAAGAC ATTCCTAAGT 
TCCTCCGAGT GGCGAGGAGT GTGGCTGGTG GCTCCCTTGA TTGCAAAACT ACCTACCTCA 
GTTCAAGGTA GGGTCTTGAA AGCTGCGGGA GAGGAGCTGG AGAAAGGTCA GCATTTGGGG 
TCATCTTCTA AGAAGGAAAG AGATAGACAG AAGCAAAAGA GCATGTCTCT CTTGAGTCAG 
CAGCCTTTCC TTTCTTTGGT ACTTACTTGC CTTAAGGGAC AGGATGAGCA GCGGGAAGGG 
CTTCTAACAT CACTGCAGAA TCAAGTCAAT CAGATCCTTA GTAACTGGAG GGAAGAACGG 
TACCAGGGTG ATGTTAAAGC TAGGCAAATG ATGCATGAAG CCTTACAGCT TCGTCTGAAT 
TTGGTAGGTG GCATGTTTGA TACTGTGCAG AGGAGTACCC AGTGGACCAC AGACTGGGCA 
CTTCTGCTCC TCCAGATAAT CACTTCGGGA ACAGTTGATA TGCAGACCAA CAACGAATTG 
TTCACAACCG TGCTGGATAT GCTGGGTGTC CTCATCAACG GGACGCTCGC CTCCGACTTG 
TCCAGTGCTT CCCAAGGGGG ACCCGAGGAA AACAAAAGGG CTTACATGAA CTTAGTGAAA 
AAGTTAAAAA AGGAGCTGGG AGACAAGCGG TCAGACAGCA TTGACAAGGT TCGCCAGCTG 
CTCCCTTTGC CAAAGCAGAC ATGTGATATT ATTACCTGTG AGCCAATGGG ATCCTTGATT 
GACACCAAGG GCAACAAAAT TGCGGGGTTT GACTCCATTG ATAAGAAACA GGGTCTTCAG 
GTGTCAACAA AACAAAAGGT GTCCCCTTGG GATTTGTTTG AAGGTCATAA AAATCCTGCT 
CCTCTCTCCT GGGCCTGGTT TGGTACAGTT CGTGTCGACA GGAAGGTGAT CAAATACGAA 
GAGCAGCAGC ATCTGCTCCT GTACCATGCG CACCCCAAAC CCAAGCCGCG GAGCTACTAC 
CTCGAGCCCC TGCCTCTGCC TCCGGAGGAG GAGGAGGAGG AGGAGCCCAC CACCCCTGTC 
TCTCAGGAAC CAGAAAGGAA GTCAGGAGAT CAGGATCAGG GAAAGCATGC CACAGATGAC 
GAGAAGAAGA CAAAGGGCAG GAAGAGGAAG TCAAAGTCAG GCTCTAAGGC TGATGAATAT 
CCACAGAACA ACTTATACAG AGTGCCTCCC AATTACTCTC CCATTCCCTC ACAAATGATG 
CAGCATCCTC AGTCTGCCCT GTGGGGCTAC ACCATCATGG GGCAGCCGCA GCAGCCTGGC 
TTCTTCGTGC AGAACCAGCC CCTCCCTCCA GGTGGTTCCA GGCTGGATCC AACGAGCTCC 
TTCGTCCCAG CCAACACGAA GCAGGCCCTG TCCAACATGC TGCAGCGGCG CTCCGGCACC 
GTGCTGCAGC CCCCTGCCCT CCACGCAGTC ACACCTCAGC AGCAGTTGCT CCAGATGAAA 
CTCCTGCAGC AAGAGCAGCA GCAGCAGCGG CTCCTCAGGC ACCAAGCACA GGGTCAGCCG 
ATGGACCCCG CTGCTCTTTT CACCCCGCAA GTCCGACCCA CGCCACAGCT ACCGCAGTAC 
CCAGGGCTTC AGCAAGCACA GAGCATGCCC CACGGGTACA CGATGTACAG CACCCCGATG 
CCTTTGCAGC AGCAGCAGCA GCCCAGCAGC GTGGTGCTCT CTCCTGGCTA CACCCCGCGG 
GCATACACAG CCCACTCCAG CCCCGTGCTG ATGGAGCGGC TGCGGCCGGG GCAGCCGGGC 
AGCGTGGCTC AGCAGCAGGC AGCTGCCTAC ATGCAGCCCC TGGCGGGTGC TCAGCGGTTG 
ACCCATCAGC CTCTGCAGCC AAACTCATTG ATAGGGGCAG GCCTGGACAG TGCATCAACC 
GCACGTACAC ATCCAACCTT GAGCTCAGCA CAGCTGCCCC AGGAGCAAAT GCGACAGAGG 
CAGCAGGAGA TCCGACAGCA GAGACTCTTC CAGATGCAGC AGGGGCAGCA GCAGCAGACT 
CTTGATCTTC AGCCTGTGCA GCCACAGCAG CCCCTGGTAA GTGGGATGGA TGGTGAGCGT 
GGGGCAGGGG CGTGGGATGA GCGGGTGAAG CTTACAGTGA ATTTGCAGTG CTGTTGTCCA 
TGTTTCCTGG ACATGGCCAT TTTTTCCAGA TATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_015291834.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147320.2 Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X5, mRNA.

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CloneID	OGa152697
Clone ID Related Accession (Same CDS sequence)	XM_015291834.2
Accession Version	XM_015291834.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291834.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAGCTGCTG 
CTGAAGTCGT CCAGCCTTGC AGGGAGCTAC ACCACGGGGC TGTGTGTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCT AAGGTGAAGC AAACGATATA CAGCAATGTT 
CAGCCTTCAA ATTCCAATCT GCTGTGGGAC CCGGAGTTCA TGCTTGATTT TATTGAGAAC 
CCTTCTGCTC ACAGCATTAA CTACTCCATG CTGGGCAAGA TCCTGAGTGA CAACGCAGCC 
AACCGCTACA GCTTTGTCTG CAATGCTCTA ATGAATGTGT GCATGGGGCA CCAAGATGCA 
GGGAGGATTA ATGACATAGC AAACCTTTGT GCAGAGCTGA CGGCGTGCTG CACCGTGCTG 
AGCTCGGAGT GGCTGGGGGT TGTCAAAGCT CTTTGCTGCT CTTCCAATCA CGTGTGGGGT 
TTCAATGATC TGCTCTGCAG CGTGGATGTG AGTGACCTGT CATTCCACGA TTCCCTGGCC 
ACTTTCATTG CCATACTGAT TGCCCGGCAG TGCTTTTCTC TGGAAGATGT CGTTCAGCAC 
GTAGCACTGC CTTCTCTTCT TGCAGCTGCC TGTGGGGACC CAGATGCTGA GCCTGGGGCC 
AGGATGACTT GTCGGCTTCT CCTGCACCTC TTCAGGACAC CCCAGGTCTG CCTCTTTCCC 
CAGGGAGCAG GAAAATTATT TCCTGGAATT CGTTCTTCTT GTGATCGTCA CCTCTTGGCT 
GCTGCTCACA ACAGCATAGA AGTGGGAGCT GTGTTTGCAG TCTTAAAAGC AATTTTGATG 
CTTGGTGACG CTGAGATCGG CAGCAGCAGT GTCACCCCTG TGAAGAACGA GGAGCTGCCC 
GTGAGGGGCT TGCTGGATGA GATCGCTGAG GATGAAATGT GGGCTTCCTC TCACGCTCTG 
AAATCACGTG GAAAAGCTGT TTCCATAGAA ACCGCCAGTT TGAGCGAGTA TGCTAGATAT 
GTGCTAAGGA CCATTTGCCA GCAGGAATGG GTTGGAGAGC ACTGTTTAAA AGAACCTGAG 
AGGCTGTGCA CAGATAAAGA TCTAATTTTG GATCCCGTTC TTTCAAACAA GCAAGCACAG 
AAACTTCTTC AGCTTATCTG TTATCCTCAT GGTGTGAAAG AATGCTCCGA GGGGGACAAC 
CCTCAGAGGC AGCACATCAA ACGCATACTT CAGAACTTAG ACCAGTGGAC TCTGCGGCAG 
TCTTGGTTGG AGCTGCAACT AATGATCAAG CAGTGCATGA AGGAGCCTGG TTCTGGGTCT 
GTGGCTGAAA TGAACAGCTT GTTGGATAAT ATTGCAAAGG CGACAATAGA GGTGTTTCAG 
CAATCCGCTG ATCTAAACAA TAACTCTTCT AACTCTGGTA TAGGCCTCTT CAATCCAAAC 
TCTGTTGGAA ATGCTGACAC AAGTAATACA AGACAGAATG GTAAAAAGAC ATTCCTAAGT 
TCCTCCGAGT GGCGAGGAGT GTGGCTGGTG GCTCCCTTGA TTGCAAAACT ACCTACCTCA 
GTTCAAGGTA GGGTCTTGAA AGCTGCGGGA GAGGAGCTGG AGAAAGGTCA GCATTTGGGG 
TCATCTTCTA AGAAGGAAAG AGATAGACAG AAGCAAAAGA GCATGTCTCT CTTGAGTCAG 
CAGCCTTTCC TTTCTTTGGT ACTTACTTGC CTTAAGGGAC AGGATGAGCA GCGGGAAGGG 
CTTCTAACAT CACTGCAGAA TCAAGTCAAT CAGATCCTTA GTAACTGGAG GGAAGAACGG 
TACCAGGGTG ATGTTAAAGC TAGGCAAATG ATGCATGAAG CCTTACAGCT TCGTCTGAAT 
TTGGTAGGTG GCATGTTTGA TACTGTGCAG AGGAGTACCC AGTGGACCAC AGACTGGGCA 
CTTCTGCTCC TCCAGATAAT CACTTCGGGA ACAGTTGATA TGCAGACCAA CAACGAATTG 
TTCACAACCG TGCTGGATAT GCTGGGTGTC CTCATCAACG GGACGCTCGC CTCCGACTTG 
TCCAGTGCTT CCCAAGGGGG ACCCGAGGAA AACAAAAGGG CTTACATGAA CTTAGTGAAA 
AAGTTAAAAA AGGAGCTGGG AGACAAGCGG TCAGACAGCA TTGACAAGGT TCGCCAGCTG 
CTCCCTTTGC CAAAGCAGAC ATGTGATATT ATTACCTGTG AGCCAATGGG ATCCTTGATT 
GACACCAAGG GCAACAAAAT TGCGGGGTTT GACTCCATTG ATAAGAAACA GGGTCTTCAG 
GTGTCAACAA AACAAAAGGT GTCCCCTTGG GATTTGTTTG AAGGTCATAA AAATCCTGCT 
CCTCTCTCCT GGGCCTGGTT TGGTACAGTT CGTGTCGACA GGAAGGTGAT CAAATACGAA 
GAGCAGCAGC ATCTGCTCCT GTACCATGCG CACCCCAAAC CCAAGCCGCG GAGCTACTAC 
CTCGAGCCCC TGCCTCTGCC TCCGGAGGAG GAGGAGGAGG AGGAGCCCAC CACCCCTGTC 
TCTCAGGAAC CAGAAAGGAA GTCAGGAGAT CAGGATCAGG GAAAGCATGC CACAGATGAC 
GAGAAGAAGA CAAAGGGCAG GAAGAGGAAG TCAAAGTCAG GCTCTAAGGC TGATGAATAT 
CCACAGAACA ACTTATACAG AGTGCCTCCC AATTACTCTC CCATTCCCTC ACAAATGATG 
CAGCATCCTC AGTCTGCCCT GTGGGGCTAC ACCATCATGG GGCAGCCGCA GCAGCCTGGC 
TTCTTCGTGC AGAACCAGCC CCTCCCTCCA GGTGGTTCCA GGCTGGATCC AACGAGCTCC 
TTCGTCCCAG CCAACACGAA GCAGGCCCTG TCCAACATGC TGCAGCGGCG CTCCGGCACC 
GTGCTGCAGC CCCCTGCCCT CCACGCAGTC ACACCTCAGC AGCAGTTGCT CCAGATGAAA 
CTCCTGCAGC AAGAGCAGCA GCAGCAGCGG CTCCTCAGGC ACCAAGCACA GGCACGGTCT 
CTCCAGCAGA GCATGCCCCA CGGGTACACG ATGTACAGCA CCCCGATGCC TTTGCAGCAG 
CAGCAGCAGC CCAGCAGCGT GGTGCTCTCT CCTGGCTACA CCCCGCGGGC ATACACAGCC 
CACTCCAGCC CCGTGCTGAT GGAGCGGCTG CGGCCGGGGC AGCCGGGCAG CGTGGCTCAG 
CAGCAGGCAG CTGCCTACAT GCAGCCCCTG GCGGGTGCTC AGCGGTTGAC CCATCAGCCT 
CTGCAGCCAA ACTCATTGAT AGGGGCAGGC CTGGACAGTG CATCAACCGC ACGTACACAT 
CCAACCTTGA GCTCAGCACA GCTGCCCCAG GAGCAAATGC GACAGAGGCA GCAGGAGATC 
CGACAGCAGA GACTCTTCCA GATGCAGCAG GGGCAGCAGC AGCAGACTCT TGATCTTCAG 
CCTGTGCAGC CACAGCAGCC CCTGGTAAGT GGGATGGATG GTGAGCGTGG GGCAGGGGCG 
TGGGATGAGC GGGTGAAGCT TACAGTGAAT TTGCAGTGCT GTTGTCCATG TTTCCTGGAC 
ATGGCCATTT TTTCCAGATA TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147320.2
CDS	33..6419
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPRQKEDELTAVNVKQGFSNQPAFSGDEHGSARNIVINASKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHNWFTDLAGSKPLTILAKKVPILSKKEDVFAYLAKYSVPLLRAAWLIKMTCAYYAAISEAKIKKRQATDPNIEWTQIITRYLREQLAKVAEFYHVTSSQGNSSVVMPQEMEQALKQWEYNEKLSFYMFQEGMLERHEYLTWILDVLEKIRPTDDDILKLVLPLMLQYSEEFVQSAYLSRRLAYFCARRLSLLLSDGPSLAAAHSPHVIIAPSNPPLAVPSPTAPGPVLSPVQLTCSDFLSCPQHRPLVYGLSCMLQTITLCCPSALVWNYSTNENKNMNPGSPLDLLQVAPSSLPMPGGNSTFNQQVRAKIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRSDSSNSMETLYHKIFWANQNKDNQEAAPSDEAVVMLLCEWAVSCKRSGKHRAMVVAKLLEKRQAEIEAERCGEAEVLDEKESLSSASLTSSSLPVFQNILLRFLDTQAPSLSDPNSDHEKTEFVNLVLLFSEFTRHDVFSHDAYMCTLISRGDLSITATTRPRSPNGETGDEHYSKDHDMKMEDHNIMEHMGTDSGPTSIFDDVDKGDFKADFGSEFPIFSPMPGESCENMNSSLDRRISVASEKSVKRERLREVIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRMILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQEAFPTLETVFTKLQQLSYFDQHQVTSQISSNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHACLILNSEQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYSNVQPSNSNLLWDPEFMLDFIENPSAHSINYSMLGKILSDNAANRYSFVCNALMNVCMGHQDAGRINDIANLCAELTACCTVLSSEWLGVVKALCCSSNHVWGFNDLLCSVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDPDAEPGARMTCRLLLHLFRTPQVCLFPQGAGKLFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAILMLGDAEIGSSSVTPVKNEELPVRGLLDEIAEDEMWASSHALKSRGKAVSIETASLSEYARYVLRTICQQEWVGEHCLKEPERLCTDKDLILDPVLSNKQAQKLLQLICYPHGVKECSEGDNPQRQHIKRILQNLDQWTLRQSWLELQLMIKQCMKEPGSGSVAEMNSLLDNIAKATIEVFQQSADLNNNSSNSGIGLFNPNSVGNADTSNTRQNGKKTFLSSSEWRGVWLVAPLIAKLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQGDVKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMQTNNELFTTVLDMLGVLINGTLASDLSSASQGGPEENKRAYMNLVKKLKKELGDKRSDSIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGHKNPAPLSWAWFGTVRVDRKVIKYEEQQHLLLYHAHPKPKPRSYYLEPLPLPPEEEEEEEPTTPVSQEPERKSGDQDQGKHATDDEKKTKGRKRKSKSGSKADEYPQNNLYRVPPNYSPIPSQMMQHPQSALWGYTIMGQPQQPGFFVQNQPLPPGGSRLDPTSSFVPANTKQALSNMLQRRSGTVLQPPALHAVTPQQQLLQMKLLQQEQQQQRLLRHQAQARSLQQSMPHGYTMYSTPMPLQQQQQPSSVVLSPGYTPRAYTAHSSPVLMERLRPGQPGSVAQQQAAAYMQPLAGAQRLTHQPLQPNSLIGAGLDSASTARTHPTLSSAQLPQEQMRQRQQEIRQQRLFQMQQGQQQQTLDLQPVQPQQPLVSGMDGERGAGAWDERVKLTVNLQCCCPCFLDMAIFSRYV

Target ORF information:

RefSeq Version	XM_015291834.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291834.2

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAGCTGCTG 
CTGAAGTCGT CCAGCCTTGC AGGGAGCTAC ACCACGGGGC TGTGTGTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCT AAGGTGAAGC AAACGATATA CAGCAATGTT 
CAGCCTTCAA ATTCCAATCT GCTGTGGGAC CCGGAGTTCA TGCTTGATTT TATTGAGAAC 
CCTTCTGCTC ACAGCATTAA CTACTCCATG CTGGGCAAGA TCCTGAGTGA CAACGCAGCC 
AACCGCTACA GCTTTGTCTG CAATGCTCTA ATGAATGTGT GCATGGGGCA CCAAGATGCA 
GGGAGGATTA ATGACATAGC AAACCTTTGT GCAGAGCTGA CGGCGTGCTG CACCGTGCTG 
AGCTCGGAGT GGCTGGGGGT TGTCAAAGCT CTTTGCTGCT CTTCCAATCA CGTGTGGGGT 
TTCAATGATC TGCTCTGCAG CGTGGATGTG AGTGACCTGT CATTCCACGA TTCCCTGGCC 
ACTTTCATTG CCATACTGAT TGCCCGGCAG TGCTTTTCTC TGGAAGATGT CGTTCAGCAC 
GTAGCACTGC CTTCTCTTCT TGCAGCTGCC TGTGGGGACC CAGATGCTGA GCCTGGGGCC 
AGGATGACTT GTCGGCTTCT CCTGCACCTC TTCAGGACAC CCCAGGTCTG CCTCTTTCCC 
CAGGGAGCAG GAAAATTATT TCCTGGAATT CGTTCTTCTT GTGATCGTCA CCTCTTGGCT 
GCTGCTCACA ACAGCATAGA AGTGGGAGCT GTGTTTGCAG TCTTAAAAGC AATTTTGATG 
CTTGGTGACG CTGAGATCGG CAGCAGCAGT GTCACCCCTG TGAAGAACGA GGAGCTGCCC 
GTGAGGGGCT TGCTGGATGA GATCGCTGAG GATGAAATGT GGGCTTCCTC TCACGCTCTG 
AAATCACGTG GAAAAGCTGT TTCCATAGAA ACCGCCAGTT TGAGCGAGTA TGCTAGATAT 
GTGCTAAGGA CCATTTGCCA GCAGGAATGG GTTGGAGAGC ACTGTTTAAA AGAACCTGAG 
AGGCTGTGCA CAGATAAAGA TCTAATTTTG GATCCCGTTC TTTCAAACAA GCAAGCACAG 
AAACTTCTTC AGCTTATCTG TTATCCTCAT GGTGTGAAAG AATGCTCCGA GGGGGACAAC 
CCTCAGAGGC AGCACATCAA ACGCATACTT CAGAACTTAG ACCAGTGGAC TCTGCGGCAG 
TCTTGGTTGG AGCTGCAACT AATGATCAAG CAGTGCATGA AGGAGCCTGG TTCTGGGTCT 
GTGGCTGAAA TGAACAGCTT GTTGGATAAT ATTGCAAAGG CGACAATAGA GGTGTTTCAG 
CAATCCGCTG ATCTAAACAA TAACTCTTCT AACTCTGGTA TAGGCCTCTT CAATCCAAAC 
TCTGTTGGAA ATGCTGACAC AAGTAATACA AGACAGAATG GTAAAAAGAC ATTCCTAAGT 
TCCTCCGAGT GGCGAGGAGT GTGGCTGGTG GCTCCCTTGA TTGCAAAACT ACCTACCTCA 
GTTCAAGGTA GGGTCTTGAA AGCTGCGGGA GAGGAGCTGG AGAAAGGTCA GCATTTGGGG 
TCATCTTCTA AGAAGGAAAG AGATAGACAG AAGCAAAAGA GCATGTCTCT CTTGAGTCAG 
CAGCCTTTCC TTTCTTTGGT ACTTACTTGC CTTAAGGGAC AGGATGAGCA GCGGGAAGGG 
CTTCTAACAT CACTGCAGAA TCAAGTCAAT CAGATCCTTA GTAACTGGAG GGAAGAACGG 
TACCAGGGTG ATGTTAAAGC TAGGCAAATG ATGCATGAAG CCTTACAGCT TCGTCTGAAT 
TTGGTAGGTG GCATGTTTGA TACTGTGCAG AGGAGTACCC AGTGGACCAC AGACTGGGCA 
CTTCTGCTCC TCCAGATAAT CACTTCGGGA ACAGTTGATA TGCAGACCAA CAACGAATTG 
TTCACAACCG TGCTGGATAT GCTGGGTGTC CTCATCAACG GGACGCTCGC CTCCGACTTG 
TCCAGTGCTT CCCAAGGGGG ACCCGAGGAA AACAAAAGGG CTTACATGAA CTTAGTGAAA 
AAGTTAAAAA AGGAGCTGGG AGACAAGCGG TCAGACAGCA TTGACAAGGT TCGCCAGCTG 
CTCCCTTTGC CAAAGCAGAC ATGTGATATT ATTACCTGTG AGCCAATGGG ATCCTTGATT 
GACACCAAGG GCAACAAAAT TGCGGGGTTT GACTCCATTG ATAAGAAACA GGGTCTTCAG 
GTGTCAACAA AACAAAAGGT GTCCCCTTGG GATTTGTTTG AAGGTCATAA AAATCCTGCT 
CCTCTCTCCT GGGCCTGGTT TGGTACAGTT CGTGTCGACA GGAAGGTGAT CAAATACGAA 
GAGCAGCAGC ATCTGCTCCT GTACCATGCG CACCCCAAAC CCAAGCCGCG GAGCTACTAC 
CTCGAGCCCC TGCCTCTGCC TCCGGAGGAG GAGGAGGAGG AGGAGCCCAC CACCCCTGTC 
TCTCAGGAAC CAGAAAGGAA GTCAGGAGAT CAGGATCAGG GAAAGCATGC CACAGATGAC 
GAGAAGAAGA CAAAGGGCAG GAAGAGGAAG TCAAAGTCAG GCTCTAAGGC TGATGAATAT 
CCACAGAACA ACTTATACAG AGTGCCTCCC AATTACTCTC CCATTCCCTC ACAAATGATG 
CAGCATCCTC AGTCTGCCCT GTGGGGCTAC ACCATCATGG GGCAGCCGCA GCAGCCTGGC 
TTCTTCGTGC AGAACCAGCC CCTCCCTCCA GGTGGTTCCA GGCTGGATCC AACGAGCTCC 
TTCGTCCCAG CCAACACGAA GCAGGCCCTG TCCAACATGC TGCAGCGGCG CTCCGGCACC 
GTGCTGCAGC CCCCTGCCCT CCACGCAGTC ACACCTCAGC AGCAGTTGCT CCAGATGAAA 
CTCCTGCAGC AAGAGCAGCA GCAGCAGCGG CTCCTCAGGC ACCAAGCACA GGCACGGTCT 
CTCCAGCAGA GCATGCCCCA CGGGTACACG ATGTACAGCA CCCCGATGCC TTTGCAGCAG 
CAGCAGCAGC CCAGCAGCGT GGTGCTCTCT CCTGGCTACA CCCCGCGGGC ATACACAGCC 
CACTCCAGCC CCGTGCTGAT GGAGCGGCTG CGGCCGGGGC AGCCGGGCAG CGTGGCTCAG 
CAGCAGGCAG CTGCCTACAT GCAGCCCCTG GCGGGTGCTC AGCGGTTGAC CCATCAGCCT 
CTGCAGCCAA ACTCATTGAT AGGGGCAGGC CTGGACAGTG CATCAACCGC ACGTACACAT 
CCAACCTTGA GCTCAGCACA GCTGCCCCAG GAGCAAATGC GACAGAGGCA GCAGGAGATC 
CGACAGCAGA GACTCTTCCA GATGCAGCAG GGGCAGCAGC AGCAGACTCT TGATCTTCAG 
CCTGTGCAGC CACAGCAGCC CCTGGTAAGT GGGATGGATG GTGAGCGTGG GGCAGGGGCG 
TGGGATGAGC GGGTGAAGCT TACAGTGAAT TTGCAGTGCT GTTGTCCATG TTTCCTGGAC 
ATGGCCATTT TTTCCAGATA TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_015291835.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147321.2 Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X6, mRNA.

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CloneID	OGa152698
Clone ID Related Accession (Same CDS sequence)	XM_015291835.2
Accession Version	XM_015291835.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291835.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGAT TTTTTCTCCG 
ATGCCTGGGG AGTCCTGTGA GAACATGAAC TCTTCTTTAG ACAGAAGAAT TTCTGTTGCC 
AGTGAGAAGT CAGTCAAGAG GGAAAGACTC AGAGAAGTGA TTTTTCCATC TAACTATGAC 
CTCCTTCGTC ATCTGCAATA TGCAACGCAT TTCCCAATAC CTCTGGATGA ATCCTCAAGC 
CATGAGTGCA ACCAGCGCAT GATTCTTCTC TATGGTGTTG GCAAGGAGCG TGATGAAGCA 
AGACATCAGC TGAAGAAGAT TACCAAAGAC ATCCTGAAGA TTCTGAATAA GAAGAGTACT 
ACTGAAACGG GTGTTGGGGA TGAAGGGCAA AAAGCCAGGA AGAACAAACA AGAAGCATTT 
CCAACTCTAG AGACTGTGTT CACAAAACTA CAGCAGCTGT CATATTTTGA CCAACATCAG 
GTGACATCTC AGATCTCCAG CAATGTTCTC GAACAAATAA CCAGCTTTGC CTCAGGAACA 
TCCTATCACC TCCCCTTGGC TCACCACATA CAGCTCATCT TTGATCTCAT GGAACCAGCA 
CTGAATATCA ACGGGCTGAT AGACTTTGCA ATACAGCTGC TGAATGAGCT GAGCGTGGTG 
GAGGCAGAGC TGCTGCTGAA GTCGTCCAGC CTTGCAGGGA GCTACACCAC GGGGCTGTGT 
GTGTGCATCG TTGCCGTCCT GCGGCGTTAC CACGCCTGCC TCATTCTCAA CTCGGAGCAA 
ACTGCACAAG TCTTTGAAGG GTTGTGTGGA GTGGTGAAGC ACGTTGTTAA TCCCTCAGAG 
TGTTCCTCCC CAGAAAGATG TATTTTGGCC TACCTCTATG ACCTGTATGT GTCCTGCAGC 
CACCTGCGAA GCAAGTTTGG AGACCTCTTC AGTAGTGCCT GTTCTAAGGT GAAGCAAACG 
ATATACAGCA ATGTTCAGCC TTCAAATTCC AATCTGCTGT GGGACCCGGA GTTCATGCTT 
GATTTTATTG AGAACCCTTC TGCTCACAGC ATTAACTACT CCATGCTGGG CAAGATCCTG 
AGTGACAACG CAGCCAACCG CTACAGCTTT GTCTGCAATG CTCTAATGAA TGTGTGCATG 
GGGCACCAAG ATGCAGGGAG GATTAATGAC ATAGCAAACC TTTGTGCAGA GCTGACGGCG 
TGCTGCACCG TGCTGAGCTC GGAGTGGCTG GGGGTTGTCA AAGCTCTTTG CTGCTCTTCC 
AATCACGTGT GGGGTTTCAA TGATCTGCTC TGCAGCGTGG ATGTGAGTGA CCTGTCATTC 
CACGATTCCC TGGCCACTTT CATTGCCATA CTGATTGCCC GGCAGTGCTT TTCTCTGGAA 
GATGTCGTTC AGCACGTAGC ACTGCCTTCT CTTCTTGCAG CTGCCTGTGG GGACCCAGAT 
GCTGAGCCTG GGGCCAGGAT GACTTGTCGG CTTCTCCTGC ACCTCTTCAG GACACCCCAG 
GTCTGCCTCT TTCCCCAGGG AGCAGGAAAA TTATTTCCTG GAATTCGTTC TTCTTGTGAT 
CGTCACCTCT TGGCTGCTGC TCACAACAGC ATAGAAGTGG GAGCTGTGTT TGCAGTCTTA 
AAAGCAATTT TGATGCTTGG TGACGCTGAG ATCGGCAGCA GCAGTGTCAC CCCTGTGAAG 
AACGAGGAGC TGCCCGTGAG GGGCTTGCTG GATGAGATCG CTGAGGATGA AATGTGGGCT 
TCCTCTCACG CTCTGAAATC ACGTGGAAAA GCTGTTTCCA TAGAAACCGC CAGTTTGAGC 
GAGTATGCTA GATATGTGCT AAGGACCATT TGCCAGCAGG AATGGGTTGG AGAGCACTGT 
TTAAAAGAAC CTGAGAGGCT GTGCACAGAT AAAGATCTAA TTTTGGATCC CGTTCTTTCA 
AACAAGCAAG CACAGAAACT TCTTCAGCTT ATCTGTTATC CTCATGGTGT GAAAGAATGC 
TCCGAGGGGG ACAACCCTCA GAGGCAGCAC ATCAAACGCA TACTTCAGAA CTTAGACCAG 
TGGACTCTGC GGCAGTCTTG GTTGGAGCTG CAACTAATGA TCAAGCAGTG CATGAAGGAG 
CCTGGTTCTG GGTCTGTGGC TGAAATGAAC AGCTTGTTGG ATAATATTGC AAAGGCGACA 
ATAGAGGTGT TTCAGCAATC CGCTGATCTA AACAATAACT CTTCTAACTC TGGTATAGGC 
CTCTTCAATC CAAACTCTGT TGGAAATGCT GACACAAGTA ATACAAGACA GAATGGTAAA 
AAGACATTCC TAAGTTCCTC CGAGTGGCGA GGAGTGTGGC TGGTGGCTCC CTTGATTGCA 
AAACTACCTA CCTCAGTTCA AGGTAGGGTC TTGAAAGCTG CGGGAGAGGA GCTGGAGAAA 
GGTCAGCATT TGGGGTCATC TTCTAAGAAG GAAAGAGATA GACAGAAGCA AAAGAGCATG 
TCTCTCTTGA GTCAGCAGCC TTTCCTTTCT TTGGTACTTA CTTGCCTTAA GGGACAGGAT 
GAGCAGCGGG AAGGGCTTCT AACATCACTG CAGAATCAAG TCAATCAGAT CCTTAGTAAC 
TGGAGGGAAG AACGGTACCA GGGTGATGTT AAAGCTAGGC AAATGATGCA TGAAGCCTTA 
CAGCTTCGTC TGAATTTGGT AGGTGGCATG TTTGATACTG TGCAGAGGAG TACCCAGTGG 
ACCACAGACT GGGCACTTCT GCTCCTCCAG ATAATCACTT CGGGAACAGT TGATATGCAG 
ACCAACAACG AATTGTTCAC AACCGTGCTG GATATGCTGG GTGTCCTCAT CAACGGGACG 
CTCGCCTCCG ACTTGTCCAG TGCTTCCCAA GGGGGACCCG AGGAAAACAA AAGGGCTTAC 
ATGAACTTAG TGAAAAAGTT AAAAAAGGAG CTGGGAGACA AGCGGTCAGA CAGCATTGAC 
AAGGTTCGCC AGCTGCTCCC TTTGCCAAAG CAGACATGTG ATATTATTAC CTGTGAGCCA 
ATGGGATCCT TGATTGACAC CAAGGGCAAC AAAATTGCGG GGTTTGACTC CATTGATAAG 
AAACAGGGTC TTCAGGTGTC AACAAAACAA AAGGTGTCCC CTTGGGATTT GTTTGAAGGT 
CATAAAAATC CTGCTCCTCT CTCCTGGGCC TGGTTTGGTA CAGTTCGTGT CGACAGGAAG 
GTGATCAAAT ACGAAGAGCA GCAGCATCTG CTCCTGTACC ATGCGCACCC CAAACCCAAG 
CCGCGGAGCT ACTACCTCGA GCCCCTGCCT CTGCCTCCGG AGGAGGAGGA GGAGGAGGAG 
CCCACCACCC CTGTCTCTCA GGAACCAGAA AGGAAGTCAG GAGATCAGGA TCAGGGAAAG 
CATGCCACAG ATGACGAGAA GAAGACAAAG GGCAGGAAGA GGAAGTCAAA GTCAGGCTCT 
AAGGCTGATG AATATCCACA GAACAACTTA TACAGAGTGC CTCCCAATTA CTCTCCCATT 
CCCTCACAAA TGATGCAGCA TCCTCAGTCT GCCCTGTGGG GCTACACCAT CATGGGGCAG 
CCGCAGCAGC CTGGCTTCTT CGTGCAGAAC CAGCCCCTCC CTCCAGGTGG TTCCAGGCTG 
GATCCAACGA GCTCCTTCGT CCCAGCCAAC ACGAAGCAGG CCCTGTCCAA CATGCTGCAG 
CGGCGCTCCG GCACCGTGCT GCAGCCCCCT GCCCTCCACG CAGTCACACC TCAGCAGCAG 
TTGCTCCAGA TGAAACTCCT GCAGCAAGAG CAGCAGCAGC AGCGGCTCCT CAGGCACCAA 
GCACAGGCAC GGTCTCTCCA GCAGGGTCAG CCGATGGACC CCGCTGCTCT TTTCACCCCG 
CAAGTCCGAC CCACGCCACA GCTACCGCAG TACCCAGGGC TTCAGCAAGC ACAGAGCATG 
CCCCACGGGT ACACGATGTA CAGCACCCCG ATGCCTTTGC AGCAGCAGCA GCAGCCCAGC 
AGCGTGGTGC TCTCTCCTGG CTACACCCCG CGGGCATACA CAGCCCACTC CAGCCCCGTG 
CTGATGGAGC GGCTGCGGCC GGGGCAGCCG GGCAGCGTGG CTCAGCAGCA GGCAGCTGCC 
TACATGCAGC CCCTGGCGGG TGCTCAGCGG TTGACCCATC AGCCTCTGCA GCCAAACTCA 
TTGATAGGGG CAGGCCTGGA CAGTGCATCA ACCGCACGTA CACATCCAAC CTTGAGCTCA 
GCACAGCTGC CCCAGGAGCA AATGCGACAG AGGCAGCAGG AGATCCGACA GCAGAGACTC 
TTCCAGATGC AGCAGGGGCA GCAGCAGCAG ACTCTTGATC TTCAGCCTGT GCAGCCACAG 
CAGCCCCTGG TAAGTGGGAT GGATGGTGAG CGTGGGGCAG GGGCGTGGGA TGAGCGGGTG 
AAGCTTACAG TGAATTTGCA GTGCTGTTGT CCATGTTTCC TGGACATGGC CATTTTTTCC 
AGATATGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147321.2
CDS	1..6372
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPRQKEDELTAVNVKQGFSNQPAFSGDEHGSARNIVINASKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHNWFTDLAGSKPLTILAKKVPILSKKEDVFAYLAKYSVPLLRAAWLIKMTCAYYAAISEAKIKKRQATDPNIEWTQIITRYLREQLAKVAEFYHVTSSQGNSSVVMPQEMEQALKQWEYNEKLSFYMFQEGMLERHEYLTWILDVLEKIRPTDDDILKLVLPLMLQYSEEFVQSAYLSRRLAYFCARRLSLLLSDGPSLAAAHSPHVIIAPSNPPLAVPSPTAPGPVLSPVQLTCSDFLSCPQHRPLVYGLSCMLQTITLCCPSALVWNYSTNENKNMNPGSPLDLLQVAPSSLPMPGGNSTFNQQVRAKIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRSDSSNSMETLYHKIFWANQNKDNQEAAPSDEAVVMLLCEWAVSCKRSGKHRAMVVAKLLEKRQAEIEAERCGEAEVLDEKESLSSASLTSSSLPVFQNILLRFLDTQAPSLSDPNSDHEKTEFVNLVLLFSEFTRHDVFSHDAYMCTLISRGDLSITATTRPRSPNGETGDEHYSKDHDMKMEIFSPMPGESCENMNSSLDRRISVASEKSVKRERLREVIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRMILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQEAFPTLETVFTKLQQLSYFDQHQVTSQISSNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHACLILNSEQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYSNVQPSNSNLLWDPEFMLDFIENPSAHSINYSMLGKILSDNAANRYSFVCNALMNVCMGHQDAGRINDIANLCAELTACCTVLSSEWLGVVKALCCSSNHVWGFNDLLCSVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDPDAEPGARMTCRLLLHLFRTPQVCLFPQGAGKLFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAILMLGDAEIGSSSVTPVKNEELPVRGLLDEIAEDEMWASSHALKSRGKAVSIETASLSEYARYVLRTICQQEWVGEHCLKEPERLCTDKDLILDPVLSNKQAQKLLQLICYPHGVKECSEGDNPQRQHIKRILQNLDQWTLRQSWLELQLMIKQCMKEPGSGSVAEMNSLLDNIAKATIEVFQQSADLNNNSSNSGIGLFNPNSVGNADTSNTRQNGKKTFLSSSEWRGVWLVAPLIAKLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQGDVKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMQTNNELFTTVLDMLGVLINGTLASDLSSASQGGPEENKRAYMNLVKKLKKELGDKRSDSIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGHKNPAPLSWAWFGTVRVDRKVIKYEEQQHLLLYHAHPKPKPRSYYLEPLPLPPEEEEEEEPTTPVSQEPERKSGDQDQGKHATDDEKKTKGRKRKSKSGSKADEYPQNNLYRVPPNYSPIPSQMMQHPQSALWGYTIMGQPQQPGFFVQNQPLPPGGSRLDPTSSFVPANTKQALSNMLQRRSGTVLQPPALHAVTPQQQLLQMKLLQQEQQQQRLLRHQAQARSLQQGQPMDPAALFTPQVRPTPQLPQYPGLQQAQSMPHGYTMYSTPMPLQQQQQPSSVVLSPGYTPRAYTAHSSPVLMERLRPGQPGSVAQQQAAAYMQPLAGAQRLTHQPLQPNSLIGAGLDSASTARTHPTLSSAQLPQEQMRQRQQEIRQQRLFQMQQGQQQQTLDLQPVQPQQPLVSGMDGERGAGAWDERVKLTVNLQCCCPCFLDMAIFSRYV

Target ORF information:

RefSeq Version	XM_015291835.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291835.2

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGAT TTTTTCTCCG 
ATGCCTGGGG AGTCCTGTGA GAACATGAAC TCTTCTTTAG ACAGAAGAAT TTCTGTTGCC 
AGTGAGAAGT CAGTCAAGAG GGAAAGACTC AGAGAAGTGA TTTTTCCATC TAACTATGAC 
CTCCTTCGTC ATCTGCAATA TGCAACGCAT TTCCCAATAC CTCTGGATGA ATCCTCAAGC 
CATGAGTGCA ACCAGCGCAT GATTCTTCTC TATGGTGTTG GCAAGGAGCG TGATGAAGCA 
AGACATCAGC TGAAGAAGAT TACCAAAGAC ATCCTGAAGA TTCTGAATAA GAAGAGTACT 
ACTGAAACGG GTGTTGGGGA TGAAGGGCAA AAAGCCAGGA AGAACAAACA AGAAGCATTT 
CCAACTCTAG AGACTGTGTT CACAAAACTA CAGCAGCTGT CATATTTTGA CCAACATCAG 
GTGACATCTC AGATCTCCAG CAATGTTCTC GAACAAATAA CCAGCTTTGC CTCAGGAACA 
TCCTATCACC TCCCCTTGGC TCACCACATA CAGCTCATCT TTGATCTCAT GGAACCAGCA 
CTGAATATCA ACGGGCTGAT AGACTTTGCA ATACAGCTGC TGAATGAGCT GAGCGTGGTG 
GAGGCAGAGC TGCTGCTGAA GTCGTCCAGC CTTGCAGGGA GCTACACCAC GGGGCTGTGT 
GTGTGCATCG TTGCCGTCCT GCGGCGTTAC CACGCCTGCC TCATTCTCAA CTCGGAGCAA 
ACTGCACAAG TCTTTGAAGG GTTGTGTGGA GTGGTGAAGC ACGTTGTTAA TCCCTCAGAG 
TGTTCCTCCC CAGAAAGATG TATTTTGGCC TACCTCTATG ACCTGTATGT GTCCTGCAGC 
CACCTGCGAA GCAAGTTTGG AGACCTCTTC AGTAGTGCCT GTTCTAAGGT GAAGCAAACG 
ATATACAGCA ATGTTCAGCC TTCAAATTCC AATCTGCTGT GGGACCCGGA GTTCATGCTT 
GATTTTATTG AGAACCCTTC TGCTCACAGC ATTAACTACT CCATGCTGGG CAAGATCCTG 
AGTGACAACG CAGCCAACCG CTACAGCTTT GTCTGCAATG CTCTAATGAA TGTGTGCATG 
GGGCACCAAG ATGCAGGGAG GATTAATGAC ATAGCAAACC TTTGTGCAGA GCTGACGGCG 
TGCTGCACCG TGCTGAGCTC GGAGTGGCTG GGGGTTGTCA AAGCTCTTTG CTGCTCTTCC 
AATCACGTGT GGGGTTTCAA TGATCTGCTC TGCAGCGTGG ATGTGAGTGA CCTGTCATTC 
CACGATTCCC TGGCCACTTT CATTGCCATA CTGATTGCCC GGCAGTGCTT TTCTCTGGAA 
GATGTCGTTC AGCACGTAGC ACTGCCTTCT CTTCTTGCAG CTGCCTGTGG GGACCCAGAT 
GCTGAGCCTG GGGCCAGGAT GACTTGTCGG CTTCTCCTGC ACCTCTTCAG GACACCCCAG 
GTCTGCCTCT TTCCCCAGGG AGCAGGAAAA TTATTTCCTG GAATTCGTTC TTCTTGTGAT 
CGTCACCTCT TGGCTGCTGC TCACAACAGC ATAGAAGTGG GAGCTGTGTT TGCAGTCTTA 
AAAGCAATTT TGATGCTTGG TGACGCTGAG ATCGGCAGCA GCAGTGTCAC CCCTGTGAAG 
AACGAGGAGC TGCCCGTGAG GGGCTTGCTG GATGAGATCG CTGAGGATGA AATGTGGGCT 
TCCTCTCACG CTCTGAAATC ACGTGGAAAA GCTGTTTCCA TAGAAACCGC CAGTTTGAGC 
GAGTATGCTA GATATGTGCT AAGGACCATT TGCCAGCAGG AATGGGTTGG AGAGCACTGT 
TTAAAAGAAC CTGAGAGGCT GTGCACAGAT AAAGATCTAA TTTTGGATCC CGTTCTTTCA 
AACAAGCAAG CACAGAAACT TCTTCAGCTT ATCTGTTATC CTCATGGTGT GAAAGAATGC 
TCCGAGGGGG ACAACCCTCA GAGGCAGCAC ATCAAACGCA TACTTCAGAA CTTAGACCAG 
TGGACTCTGC GGCAGTCTTG GTTGGAGCTG CAACTAATGA TCAAGCAGTG CATGAAGGAG 
CCTGGTTCTG GGTCTGTGGC TGAAATGAAC AGCTTGTTGG ATAATATTGC AAAGGCGACA 
ATAGAGGTGT TTCAGCAATC CGCTGATCTA AACAATAACT CTTCTAACTC TGGTATAGGC 
CTCTTCAATC CAAACTCTGT TGGAAATGCT GACACAAGTA ATACAAGACA GAATGGTAAA 
AAGACATTCC TAAGTTCCTC CGAGTGGCGA GGAGTGTGGC TGGTGGCTCC CTTGATTGCA 
AAACTACCTA CCTCAGTTCA AGGTAGGGTC TTGAAAGCTG CGGGAGAGGA GCTGGAGAAA 
GGTCAGCATT TGGGGTCATC TTCTAAGAAG GAAAGAGATA GACAGAAGCA AAAGAGCATG 
TCTCTCTTGA GTCAGCAGCC TTTCCTTTCT TTGGTACTTA CTTGCCTTAA GGGACAGGAT 
GAGCAGCGGG AAGGGCTTCT AACATCACTG CAGAATCAAG TCAATCAGAT CCTTAGTAAC 
TGGAGGGAAG AACGGTACCA GGGTGATGTT AAAGCTAGGC AAATGATGCA TGAAGCCTTA 
CAGCTTCGTC TGAATTTGGT AGGTGGCATG TTTGATACTG TGCAGAGGAG TACCCAGTGG 
ACCACAGACT GGGCACTTCT GCTCCTCCAG ATAATCACTT CGGGAACAGT TGATATGCAG 
ACCAACAACG AATTGTTCAC AACCGTGCTG GATATGCTGG GTGTCCTCAT CAACGGGACG 
CTCGCCTCCG ACTTGTCCAG TGCTTCCCAA GGGGGACCCG AGGAAAACAA AAGGGCTTAC 
ATGAACTTAG TGAAAAAGTT AAAAAAGGAG CTGGGAGACA AGCGGTCAGA CAGCATTGAC 
AAGGTTCGCC AGCTGCTCCC TTTGCCAAAG CAGACATGTG ATATTATTAC CTGTGAGCCA 
ATGGGATCCT TGATTGACAC CAAGGGCAAC AAAATTGCGG GGTTTGACTC CATTGATAAG 
AAACAGGGTC TTCAGGTGTC AACAAAACAA AAGGTGTCCC CTTGGGATTT GTTTGAAGGT 
CATAAAAATC CTGCTCCTCT CTCCTGGGCC TGGTTTGGTA CAGTTCGTGT CGACAGGAAG 
GTGATCAAAT ACGAAGAGCA GCAGCATCTG CTCCTGTACC ATGCGCACCC CAAACCCAAG 
CCGCGGAGCT ACTACCTCGA GCCCCTGCCT CTGCCTCCGG AGGAGGAGGA GGAGGAGGAG 
CCCACCACCC CTGTCTCTCA GGAACCAGAA AGGAAGTCAG GAGATCAGGA TCAGGGAAAG 
CATGCCACAG ATGACGAGAA GAAGACAAAG GGCAGGAAGA GGAAGTCAAA GTCAGGCTCT 
AAGGCTGATG AATATCCACA GAACAACTTA TACAGAGTGC CTCCCAATTA CTCTCCCATT 
CCCTCACAAA TGATGCAGCA TCCTCAGTCT GCCCTGTGGG GCTACACCAT CATGGGGCAG 
CCGCAGCAGC CTGGCTTCTT CGTGCAGAAC CAGCCCCTCC CTCCAGGTGG TTCCAGGCTG 
GATCCAACGA GCTCCTTCGT CCCAGCCAAC ACGAAGCAGG CCCTGTCCAA CATGCTGCAG 
CGGCGCTCCG GCACCGTGCT GCAGCCCCCT GCCCTCCACG CAGTCACACC TCAGCAGCAG 
TTGCTCCAGA TGAAACTCCT GCAGCAAGAG CAGCAGCAGC AGCGGCTCCT CAGGCACCAA 
GCACAGGCAC GGTCTCTCCA GCAGGGTCAG CCGATGGACC CCGCTGCTCT TTTCACCCCG 
CAAGTCCGAC CCACGCCACA GCTACCGCAG TACCCAGGGC TTCAGCAAGC ACAGAGCATG 
CCCCACGGGT ACACGATGTA CAGCACCCCG ATGCCTTTGC AGCAGCAGCA GCAGCCCAGC 
AGCGTGGTGC TCTCTCCTGG CTACACCCCG CGGGCATACA CAGCCCACTC CAGCCCCGTG 
CTGATGGAGC GGCTGCGGCC GGGGCAGCCG GGCAGCGTGG CTCAGCAGCA GGCAGCTGCC 
TACATGCAGC CCCTGGCGGG TGCTCAGCGG TTGACCCATC AGCCTCTGCA GCCAAACTCA 
TTGATAGGGG CAGGCCTGGA CAGTGCATCA ACCGCACGTA CACATCCAAC CTTGAGCTCA 
GCACAGCTGC CCCAGGAGCA AATGCGACAG AGGCAGCAGG AGATCCGACA GCAGAGACTC 
TTCCAGATGC AGCAGGGGCA GCAGCAGCAG ACTCTTGATC TTCAGCCTGT GCAGCCACAG 
CAGCCCCTGG TAAGTGGGAT GGATGGTGAG CGTGGGGCAG GGGCGTGGGA TGAGCGGGTG 
AAGCTTACAG TGAATTTGCA GTGCTGTTGT CCATGTTTCC TGGACATGGC CATTTTTTCC 
AGATATGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_015291836.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147322.2 Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X7, mRNA.

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CloneID	OGa152699
Clone ID Related Accession (Same CDS sequence)	XM_015291836.2
Accession Version	XM_015291836.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291836.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAGCTGCTG 
CTGAAGTCGT CCAGCCTTGC AGGGAGCTAC ACCACGGGGC TGTGTGTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCT AAGGTGAAGC AAACGATATA CAGCAATGTT 
CAGCCTTCAA ATTCCAATCT GCTGTGGGAC CCGGAGTTCA TGCTTGATTT TATTGAGAAC 
CCTTCTGCTC ACAGCATTAA CTACTCCATG CTGGGCAAGA TCCTGAGTGA CAACGCAGCC 
AACCGCTACA GCTTTGTCTG CAATGCTCTA ATGAATGTGT GCATGGGGCA CCAAGATGCA 
GGGAGGATTA ATGACATAGC AAACCTTTGT GCAGAGCTGA CGGCGTGCTG CACCGTGCTG 
AGCTCGGAGT GGCTGGGGGT TGTCAAAGCT CTTTGCTGCT CTTCCAATCA CGTGTGGGGT 
TTCAATGATC TGCTCTGCAG CGTGGATGTG AGTGACCTGT CATTCCACGA TTCCCTGGCC 
ACTTTCATTG CCATACTGAT TGCCCGGCAG TGCTTTTCTC TGGAAGATGT CGTTCAGCAC 
GTAGCACTGC CTTCTCTTCT TGCAGCTGCC TGTGGGGACC CAGATGCTGA GCCTGGGGCC 
AGGATGACTT GTCGGCTTCT CCTGCACCTC TTCAGGACAC CCCAGGTCTG CCTCTTTCCC 
CAGGGAGCAG GAAAATTATT TCCTGGAATT CGTTCTTCTT GTGATCGTCA CCTCTTGGCT 
GCTGCTCACA ACAGCATAGA AGTGGGAGCT GTGTTTGCAG TCTTAAAAGC AATTTTGATG 
CTTGGTGACG CTGAGATCGG CAGCAGCAGT GTCACCCCTG TGAAGAACGA GGAGCTGCCC 
GTGAGGGGCT TGCTGGATGA GATCGCTGAG GATGAAATGT GGGCTTCCTC TCACGCTCTG 
AAATCACGTG GAAAAGCTGT TTCCATAGAA ACCGCCAGTT TGAGCGAGTA TGCTAGATAT 
GTGCTAAGGA CCATTTGCCA GCAGGAATGG GTTGGAGAGC ACTGTTTAAA AGAACCTGAG 
AGGCTGTGCA CAGATAAAGA TCTAATTTTG GATCCCGTTC TTTCAAACAA GCAAGCACAG 
AAACTTCTTC AGCTTATCTG TTATCCTCAT GGTGTGAAAG AATGCTCCGA GGGGGACAAC 
CCTCAGAGGC AGCACATCAA ACGCATACTT CAGAACTTAG ACCAGTGGAC TCTGCGGCAG 
TCTTGGTTGG AGCTGCAACT AATGATCAAG CAGTGCATGA AGGAGCCTGG TTCTGGGTCT 
GTGGCTGAAA TGAACAGCTT GTTGGATAAT ATTGCAAAGG CGACAATAGA GGTGTTTCAG 
CAATCCGCTG ATCTAAACAA TAACTCTTCT AACTCTGGTA TAGGCCTCTT CAATCCAAAC 
TCTGTTGGAA ATGCTGACAC AAGTAATACA AGACAGAATG GTAAAAAGAC ATTCCTAAGT 
TCCTCCGAGT GGCGAGGAGT GTGGCTGGTG GCTCCCTTGA TTGCAAAACT ACCTACCTCA 
GTTCAAGGTA GGGTCTTGAA AGCTGCGGGA GAGGAGCTGG AGAAAGGTCA GCATTTGGGG 
TCATCTTCTA AGAAGGAAAG AGATAGACAG AAGCAAAAGA GCATGTCTCT CTTGAGTCAG 
CAGCCTTTCC TTTCTTTGGT ACTTACTTGC CTTAAGGGAC AGGATGAGCA GCGGGAAGGG 
CTTCTAACAT CACTGCAGAA TCAAGTCAAT CAGATCCTTA GTAACTGGAG GGAAGAACGG 
TACCAGGGTG ATGTTAAAGC TAGGCAAATG ATGCATGAAG CCTTACAGCT TCGTCTGAAT 
TTGGTAGGTG GCATGTTTGA TACTGTGCAG AGGAGTACCC AGTGGACCAC AGACTGGGCA 
CTTCTGCTCC TCCAGATAAT CACTTCGGGA ACAGTTGATA TGCAGACCAA CAACGAATTG 
TTCACAACCG TGCTGGATAT GCTGGGTGTC CTCATCAACG GGACGCTCGC CTCCGACTTG 
TCCAGTGCTT CCCAAGGGGG ACCCGAGGAA AACAAAAGGG CTTACATGAA CTTAGTGAAA 
AAGTTAAAAA AGGAGCTGGG AGACAAGCGG TCAGACAGCA TTGACAAGGT TCGCCAGCTG 
CTCCCTTTGC CAAAGCAGAC ATGTGATATT ATTACCTGTG AGCCAATGGG ATCCTTGATT 
GACACCAAGG GCAACAAAAT TGCGGGGTTT GACTCCATTG ATAAGAAACA GGGTCTTCAG 
GTGTCAACAA AACAAAAGGT GTCCCCTTGG GATTTGTTTG AAGGTCATAA AAATCCTGCT 
CCTCTCTCCT GGGCCTGGTT TGGTACAGTT CGTGTCGACA GGAAGGTGAT CAAATACGAA 
GAGCAGCAGC ATCTGCTCCT GTACCATGCG CACCCCAAAC CCAAGCCGCG GAGCTACTAC 
CTCGAGCCCC TGCCTCTGCC TCCGGAGGAG GAGGAGGAGG AGGAGCCCAC CACCCCTGTC 
TCTCAGGAAC CAGAAAGGAA GTCAGGAGAT CAGGATCAGG GAAAGCATGC CACAGATGAC 
GAGAAGAAGA CAAAGGGCAG GAAGAGGAAG TCAAAGTCAG GCTCTAAGGC TGATGAATAT 
CCACAGAACA ACTTATACAG AGTGCCTCCC AATTACTCTC CCATTCCCTC ACAAATGATG 
CAGCATCCTC AGTCTGCCCT GTGGGGCTAC ACCATCATGG GGCAGCCGCA GCAGCCTGGC 
TTCTTCGTGC AGAACCAGCC CCTCCCTCCA GGTGGTTCCA GGCTGGATCC AACGAGCTCC 
TTCGTCCCAG CCAACACGAA GCAGGCCCTG TCCAACATGC TGCAGCGGCG CTCCGGCACC 
GTGCTGCAGC CCCCTGCCCT CCACGCAGTC ACACCTCAGC AGCAGTTGCT CCAGATGAAA 
CTCCTGCAGC AAGAGCAGCA GCAGCAGCGG CTCCTCAGGC ACCAAGCACA GGCACGGTCT 
CTCCAGCAGG ATGTCAGTAG CTCTGCCGTA TCCCTTCAGG ATGGCTTTGA CAACATAATG 
GGTCAGCCGA TGGACCCCGC TGCTCTTTTC ACCCCGCAAG TCCGACCCAC GCCACAGCTA 
CCGCAGTACC CAGGGCTTCA GCAAGCACAG GTACTGCACT GCTCCACAGA AGCATGCCCC 
ACGGGTACAC GATGTACAGC ACCCCGATGC CTTTGCAGCA GCAGCAGCAG CCCAGCAGCG 
TGGTGCTCTC TCCTGGCTAC ACCCCGCGGG CATACACAGC CCACTCCAGC CCCGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147322.2
CDS	33..6152
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPRQKEDELTAVNVKQGFSNQPAFSGDEHGSARNIVINASKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHNWFTDLAGSKPLTILAKKVPILSKKEDVFAYLAKYSVPLLRAAWLIKMTCAYYAAISEAKIKKRQATDPNIEWTQIITRYLREQLAKVAEFYHVTSSQGNSSVVMPQEMEQALKQWEYNEKLSFYMFQEGMLERHEYLTWILDVLEKIRPTDDDILKLVLPLMLQYSEEFVQSAYLSRRLAYFCARRLSLLLSDGPSLAAAHSPHVIIAPSNPPLAVPSPTAPGPVLSPVQLTCSDFLSCPQHRPLVYGLSCMLQTITLCCPSALVWNYSTNENKNMNPGSPLDLLQVAPSSLPMPGGNSTFNQQVRAKIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRSDSSNSMETLYHKIFWANQNKDNQEAAPSDEAVVMLLCEWAVSCKRSGKHRAMVVAKLLEKRQAEIEAERCGEAEVLDEKESLSSASLTSSSLPVFQNILLRFLDTQAPSLSDPNSDHEKTEFVNLVLLFSEFTRHDVFSHDAYMCTLISRGDLSITATTRPRSPNGETGDEHYSKDHDMKMEDHNIMEHMGTDSGPTSIFDDVDKGDFKADFGSEFPIFSPMPGESCENMNSSLDRRISVASEKSVKRERLREVIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRMILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQEAFPTLETVFTKLQQLSYFDQHQVTSQISSNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHACLILNSEQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYSNVQPSNSNLLWDPEFMLDFIENPSAHSINYSMLGKILSDNAANRYSFVCNALMNVCMGHQDAGRINDIANLCAELTACCTVLSSEWLGVVKALCCSSNHVWGFNDLLCSVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDPDAEPGARMTCRLLLHLFRTPQVCLFPQGAGKLFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAILMLGDAEIGSSSVTPVKNEELPVRGLLDEIAEDEMWASSHALKSRGKAVSIETASLSEYARYVLRTICQQEWVGEHCLKEPERLCTDKDLILDPVLSNKQAQKLLQLICYPHGVKECSEGDNPQRQHIKRILQNLDQWTLRQSWLELQLMIKQCMKEPGSGSVAEMNSLLDNIAKATIEVFQQSADLNNNSSNSGIGLFNPNSVGNADTSNTRQNGKKTFLSSSEWRGVWLVAPLIAKLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQGDVKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMQTNNELFTTVLDMLGVLINGTLASDLSSASQGGPEENKRAYMNLVKKLKKELGDKRSDSIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGHKNPAPLSWAWFGTVRVDRKVIKYEEQQHLLLYHAHPKPKPRSYYLEPLPLPPEEEEEEEPTTPVSQEPERKSGDQDQGKHATDDEKKTKGRKRKSKSGSKADEYPQNNLYRVPPNYSPIPSQMMQHPQSALWGYTIMGQPQQPGFFVQNQPLPPGGSRLDPTSSFVPANTKQALSNMLQRRSGTVLQPPALHAVTPQQQLLQMKLLQQEQQQQRLLRHQAQARSLQQDVSSSAVSLQDGFDNIMGQPMDPAALFTPQVRPTPQLPQYPGLQQAQVLHCSTEACPTGTRCTAPRCLCSSSSSPAAWCSLLATPRGHTQPTPAPC

Target ORF information:

RefSeq Version	XM_015291836.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mediator complex subunit 12 like (MED12L), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291836.2

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAGCTGCTG 
CTGAAGTCGT CCAGCCTTGC AGGGAGCTAC ACCACGGGGC TGTGTGTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCT AAGGTGAAGC AAACGATATA CAGCAATGTT 
CAGCCTTCAA ATTCCAATCT GCTGTGGGAC CCGGAGTTCA TGCTTGATTT TATTGAGAAC 
CCTTCTGCTC ACAGCATTAA CTACTCCATG CTGGGCAAGA TCCTGAGTGA CAACGCAGCC 
AACCGCTACA GCTTTGTCTG CAATGCTCTA ATGAATGTGT GCATGGGGCA CCAAGATGCA 
GGGAGGATTA ATGACATAGC AAACCTTTGT GCAGAGCTGA CGGCGTGCTG CACCGTGCTG 
AGCTCGGAGT GGCTGGGGGT TGTCAAAGCT CTTTGCTGCT CTTCCAATCA CGTGTGGGGT 
TTCAATGATC TGCTCTGCAG CGTGGATGTG AGTGACCTGT CATTCCACGA TTCCCTGGCC 
ACTTTCATTG CCATACTGAT TGCCCGGCAG TGCTTTTCTC TGGAAGATGT CGTTCAGCAC 
GTAGCACTGC CTTCTCTTCT TGCAGCTGCC TGTGGGGACC CAGATGCTGA GCCTGGGGCC 
AGGATGACTT GTCGGCTTCT CCTGCACCTC TTCAGGACAC CCCAGGTCTG CCTCTTTCCC 
CAGGGAGCAG GAAAATTATT TCCTGGAATT CGTTCTTCTT GTGATCGTCA CCTCTTGGCT 
GCTGCTCACA ACAGCATAGA AGTGGGAGCT GTGTTTGCAG TCTTAAAAGC AATTTTGATG 
CTTGGTGACG CTGAGATCGG CAGCAGCAGT GTCACCCCTG TGAAGAACGA GGAGCTGCCC 
GTGAGGGGCT TGCTGGATGA GATCGCTGAG GATGAAATGT GGGCTTCCTC TCACGCTCTG 
AAATCACGTG GAAAAGCTGT TTCCATAGAA ACCGCCAGTT TGAGCGAGTA TGCTAGATAT 
GTGCTAAGGA CCATTTGCCA GCAGGAATGG GTTGGAGAGC ACTGTTTAAA AGAACCTGAG 
AGGCTGTGCA CAGATAAAGA TCTAATTTTG GATCCCGTTC TTTCAAACAA GCAAGCACAG 
AAACTTCTTC AGCTTATCTG TTATCCTCAT GGTGTGAAAG AATGCTCCGA GGGGGACAAC 
CCTCAGAGGC AGCACATCAA ACGCATACTT CAGAACTTAG ACCAGTGGAC TCTGCGGCAG 
TCTTGGTTGG AGCTGCAACT AATGATCAAG CAGTGCATGA AGGAGCCTGG TTCTGGGTCT 
GTGGCTGAAA TGAACAGCTT GTTGGATAAT ATTGCAAAGG CGACAATAGA GGTGTTTCAG 
CAATCCGCTG ATCTAAACAA TAACTCTTCT AACTCTGGTA TAGGCCTCTT CAATCCAAAC 
TCTGTTGGAA ATGCTGACAC AAGTAATACA AGACAGAATG GTAAAAAGAC ATTCCTAAGT 
TCCTCCGAGT GGCGAGGAGT GTGGCTGGTG GCTCCCTTGA TTGCAAAACT ACCTACCTCA 
GTTCAAGGTA GGGTCTTGAA AGCTGCGGGA GAGGAGCTGG AGAAAGGTCA GCATTTGGGG 
TCATCTTCTA AGAAGGAAAG AGATAGACAG AAGCAAAAGA GCATGTCTCT CTTGAGTCAG 
CAGCCTTTCC TTTCTTTGGT ACTTACTTGC CTTAAGGGAC AGGATGAGCA GCGGGAAGGG 
CTTCTAACAT CACTGCAGAA TCAAGTCAAT CAGATCCTTA GTAACTGGAG GGAAGAACGG 
TACCAGGGTG ATGTTAAAGC TAGGCAAATG ATGCATGAAG CCTTACAGCT TCGTCTGAAT 
TTGGTAGGTG GCATGTTTGA TACTGTGCAG AGGAGTACCC AGTGGACCAC AGACTGGGCA 
CTTCTGCTCC TCCAGATAAT CACTTCGGGA ACAGTTGATA TGCAGACCAA CAACGAATTG 
TTCACAACCG TGCTGGATAT GCTGGGTGTC CTCATCAACG GGACGCTCGC CTCCGACTTG 
TCCAGTGCTT CCCAAGGGGG ACCCGAGGAA AACAAAAGGG CTTACATGAA CTTAGTGAAA 
AAGTTAAAAA AGGAGCTGGG AGACAAGCGG TCAGACAGCA TTGACAAGGT TCGCCAGCTG 
CTCCCTTTGC CAAAGCAGAC ATGTGATATT ATTACCTGTG AGCCAATGGG ATCCTTGATT 
GACACCAAGG GCAACAAAAT TGCGGGGTTT GACTCCATTG ATAAGAAACA GGGTCTTCAG 
GTGTCAACAA AACAAAAGGT GTCCCCTTGG GATTTGTTTG AAGGTCATAA AAATCCTGCT 
CCTCTCTCCT GGGCCTGGTT TGGTACAGTT CGTGTCGACA GGAAGGTGAT CAAATACGAA 
GAGCAGCAGC ATCTGCTCCT GTACCATGCG CACCCCAAAC CCAAGCCGCG GAGCTACTAC 
CTCGAGCCCC TGCCTCTGCC TCCGGAGGAG GAGGAGGAGG AGGAGCCCAC CACCCCTGTC 
TCTCAGGAAC CAGAAAGGAA GTCAGGAGAT CAGGATCAGG GAAAGCATGC CACAGATGAC 
GAGAAGAAGA CAAAGGGCAG GAAGAGGAAG TCAAAGTCAG GCTCTAAGGC TGATGAATAT 
CCACAGAACA ACTTATACAG AGTGCCTCCC AATTACTCTC CCATTCCCTC ACAAATGATG 
CAGCATCCTC AGTCTGCCCT GTGGGGCTAC ACCATCATGG GGCAGCCGCA GCAGCCTGGC 
TTCTTCGTGC AGAACCAGCC CCTCCCTCCA GGTGGTTCCA GGCTGGATCC AACGAGCTCC 
TTCGTCCCAG CCAACACGAA GCAGGCCCTG TCCAACATGC TGCAGCGGCG CTCCGGCACC 
GTGCTGCAGC CCCCTGCCCT CCACGCAGTC ACACCTCAGC AGCAGTTGCT CCAGATGAAA 
CTCCTGCAGC AAGAGCAGCA GCAGCAGCGG CTCCTCAGGC ACCAAGCACA GGCACGGTCT 
CTCCAGCAGG ATGTCAGTAG CTCTGCCGTA TCCCTTCAGG ATGGCTTTGA CAACATAATG 
GGTCAGCCGA TGGACCCCGC TGCTCTTTTC ACCCCGCAAG TCCGACCCAC GCCACAGCTA 
CCGCAGTACC CAGGGCTTCA GCAAGCACAG GTACTGCACT GCTCCACAGA AGCATGCCCC 
ACGGGTACAC GATGTACAGC ACCCCGATGC CTTTGCAGCA GCAGCAGCAG CCCAGCAGCG 
TGGTGCTCTC TCCTGGCTAC ACCCCGCGGG CATACACAGC CCACTCCAGC CCCGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_015291836.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147322.1 Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X9, mRNA.

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CloneID	OGa40175
Clone ID Related Accession (Same CDS sequence)	XM_015291836.1
Accession Version	XM_015291836.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAACTGCTG 
TTGAAATCCT CCGACCTTGT TGGCAGCTAC ACCACCAGCT TGTGCCTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCT AAGGTGAAGC AAACGATATA CAGCAATGTT 
CAGCCTTCAA ATTCCAATCT GCTGTGGGAC CCGGAGTTCA TGCTTGATTT TATTGAGAAC 
CCTTCTGCTC ACAGCATTAA CTACTCCATG CTGGGCAAGA TCCTGAGTGA CAACGCAGCC 
AACCGCTACA GCTTTGTCTG CAATGCTCTA ATGAATGTGT GCATGGGGCA CCAAGATGCA 
GGGAGGATTA ATGACATAGC AAACCTTTGT GCAGAGCTGA CGGCGTGCTG CACCGTGCTG 
AGCTCGGAGT GGCTGGGGGT TGTCAAAGCT CTTTGCTGCT CTTCCAATCA CGTGTGGGGT 
TTCAATGATC TGCTCTGCAG CGTGGATGTG AGTGACCTGT CATTCCACGA TTCCCTGGCC 
ACTTTCATTG CCATACTGAT TGCCCGGCAG TGCTTTTCTC TGGAAGATGT CGTTCAGCAC 
GTAGCACTGC CTTCTCTTCT TGCAGCTGCC TGTGGGGACC CAGATGCTGA GCCTGGGGCC 
AGGATGACTT GTCGGCTTCT CCTGCACCTC TTCAGGACAC CCCAGGTCTG CCTCTTTCCC 
CAGGGAGCAG GAAAATTATT TCCTGGAATT CGTTCTTCTT GTGATCGTCA CCTCTTGGCT 
GCTGCTCACA ACAGCATAGA AGTGGGAGCT GTGTTTGCAG TCTTAAAAGC AATTTTGATG 
CTTGGTGACG CTGAGATCGG CAGCAGCAGT GTCACCCCTG TGAAGAACGA GGAGCTGCCC 
GTGAGGGGCT TGCTGGATGA GATCGCTGAG GATGAAATGT GGGCTTCCTC TCACGCTCTG 
AAATCACGTG GAAAAGCTGT TTCCATAGAA ACCGCCAGTT TGAGCGAGTA TGCTAGATAT 
GTGCTAAGGA CCATTTGCCA GCAGGAATGG GTTGGAGAGC ACTGTTTAAA AGAACCTGAG 
AGGCTGTGCA CAGATAAAGA TCTAATTTTG GATCCCGTTC TTTCAAACAA GCAAGCACAG 
AAACTTCTTC AGCTTATCTG TTATCCTCAT GGTGTGAAAG AATGCTCCGA GGGGGACAAC 
CCTCAGAGGC AGCACATCAA ACGCATACTT CAGAACTTAG ACCAGTGGAC TCTGCGGCAG 
TCTTGGTTGG AGCTGCAACT AATGATCAAG CAGTGCATGA AGGAGCCTGG TTCTGGGTCT 
GTGGCTGAAA TGAACAGCTT GTTGGATAAT ATTGCAAAGG CGACAATAGA GGTGTTTCAG 
CAATCCGCTG ATCTAAACAA TAACTCTTCT AACTCTGGTA TAGGCCTCTT CAATCCAAAC 
TCTGTTGGAA ATGCTGACAC AAGTAATACA AGACAGAATG GTAAAAAGAC ATTCCTAAGT 
TCCTCCGAGT GGCGAGGAGT GTGGCTGGTG GCTCCCTTGA TTGCAAAACT ACCTACCTCA 
GTTCAAGGTA GGGTCTTGAA AGCTGCGGGA GAGGAGCTGG AGAAAGGTCA GCATTTGGGG 
TCATCTTCTA AGAAGGAAAG AGATAGACAG AAGCAAAAGA GCATGTCTCT CTTGAGTCAG 
CAGCCTTTCC TTTCTTTGGT ACTTACTTGC CTTAAGGGAC AGGATGAGCA GCGGGAAGGG 
CTTCTAACAT CACTGCAGAA TCAAGTCAAT CAGATCCTTA GTAACTGGAG GGAAGAACGG 
TACCAGGGTG ATGTTAAAGC TAGGCAAATG ATGCATGAAG CCTTACAGCT TCGTCTGAAT 
TTGGTAGGTG GCATGTTTGA TACTGTGCAG AGGAGTACCC AGTGGACCAC AGACTGGGCA 
CTTCTGCTCC TCCAGATAAT CACTTCGGGA ACAGTTGATA TGCAGACCAA CAACGAATTG 
TTCACAACCG TGCTGGATAT GCTGGGTGTC CTCATCAACG GGACGCTCGC CTCCGACTTG 
TCCAGTGCTT CCCAAGGGGG ACCCGAGGAA AACAAAAGGG CTTACATGAA CTTAGTGAAA 
AAGTTAAAAA AGGAGCTGGG AGACAAGCGG TCAGACAGCA TTGACAAGGT TCGCCAGCTG 
CTCCCTTTGC CAAAGCAGAC ATGTGATATT ATTACCTGTG AGCCAATGGG ATCCTTGATT 
GACACCAAGG GCAACAAAAT TGCGGGGTTT GACTCCATTG ATAAGAAACA GGGTCTTCAG 
GTGTCAACAA AACAAAAGGT GTCCCCTTGG GATTTGTTTG AAGGTCATAA AAATCCTGCT 
CCTCTCTCCT GGGCCTGGTT TGGTACAGTT CGTGTCGACA GGAAGGTGAT CAAATACGAA 
GAGCAGCAGC ATCTGCTCCT GTACCATGCG CACCCCAAAC CCAAGCCGCG GAGCTACTAC 
CTCGAGCCCC TGCCTCTGCC TCCGGAGGAG GAGGAGGAGG AGGAGCCCAC CACCCCTGTC 
TCTCAGGAAC CAGAAAGGAA GTCAGGAGAT CAGGATCAGG GAAAGCATGC CACAGATGAC 
GAGAAGAAGA CAAAGGGCAG GAAGAGGAAG TCAAAGTCAG GCTCTAAGGC TGATGAATAT 
CCACAGAACA ACTTATACAG AGTGCCTCCC AATTACTCTC CCATTCCCTC ACAAATGATG 
CAGCATCCTC AGTCTGCCCT GTGGGGCTAC ACCATCATGG GGCAGCCGCA GCAGCCTGGC 
TTCTTCGTGC AGAACCAGCC CCTCCCTCCA GGTGGTTCCA GGCTGGATCC AACGAGCTCC 
TTCGTCCCAG CCAACACGAA GCAGGCCCTG TCCAACATGC TGCAGCGGCG CTCCGGCACC 
GTGCTGCAGC CCCCTGCCCT CCACGCAGTC ACACCTCAGC AGCAGTTGCT CCAGATGAAA 
CTCCTGCAGC AAGAGCAGCA GCAGCAGCGG CTCCTCAGGC ACCAAGCACA GGCACGGTCT 
CTCCAGCAGG ATGTCAGTAG CTCTGCCGTA TCCCTTCAGG ATGGCTTTGA CAACATAATG 
GGTCAGCCGA TGGACCCCGC TGCTCTTTTC ACCCCGCAAG TCCGACCCAC GCCACAGCTA 
CCGCAGTACC CAGGGCTTCA GCAAGCACAG GTACTGCACT GCTCCACAGA AGCATGCCCC 
ACGGGTACAC GATGTACAGC ACCCCGATGC CTTTGCAGCA GCAGCAGCAG CCCAGCAGCG 
TGGTGCTCTC TCCTGGCTAC ACCCCGCGGG CATACACAGC CCACTCCAGC CCCGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147322.1
CDS	143..6262
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPRQKEDELTAVNVKQGFSNQPAFSGDEHGSARNIVINASKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHNWFTDLAGSKPLTILAKKVPILSKKEDVFAYLAKYSVPLLRAAWLIKMTCAYYAAISEAKIKKRQATDPNIEWTQIITRYLREQLAKVAEFYHVTSSQGNSSVVMPQEMEQALKQWEYNEKLSFYMFQEGMLERHEYLTWILDVLEKIRPTDDDILKLVLPLMLQYSEEFVQSAYLSRRLAYFCARRLSLLLSDGPSLAAAHSPHVIIAPSNPPLAVPSPTAPGPVLSPVQLTCSDFLSCPQHRPLVYGLSCMLQTITLCCPSALVWNYSTNENKNMNPGSPLDLLQVAPSSLPMPGGNSTFNQQVRAKIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRSDSSNSMETLYHKIFWANQNKDNQEAAPSDEAVVMLLCEWAVSCKRSGKHRAMVVAKLLEKRQAEIEAERCGEAEVLDEKESLSSASLTSSSLPVFQNILLRFLDTQAPSLSDPNSDHEKTEFVNLVLLFSEFTRHDVFSHDAYMCTLISRGDLSITATTRPRSPNGETGDEHYSKDHDMKMEDHNIMEHMGTDSGPTSIFDDVDKGDFKADFGSEFPIFSPMPGESCENMNSSLDRRISVASEKSVKRERLREVIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRMILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQEAFPTLETVFTKLQQLSYFDQHQVTSQISSNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRRYHACLILNSEQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYSNVQPSNSNLLWDPEFMLDFIENPSAHSINYSMLGKILSDNAANRYSFVCNALMNVCMGHQDAGRINDIANLCAELTACCTVLSSEWLGVVKALCCSSNHVWGFNDLLCSVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDPDAEPGARMTCRLLLHLFRTPQVCLFPQGAGKLFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAILMLGDAEIGSSSVTPVKNEELPVRGLLDEIAEDEMWASSHALKSRGKAVSIETASLSEYARYVLRTICQQEWVGEHCLKEPERLCTDKDLILDPVLSNKQAQKLLQLICYPHGVKECSEGDNPQRQHIKRILQNLDQWTLRQSWLELQLMIKQCMKEPGSGSVAEMNSLLDNIAKATIEVFQQSADLNNNSSNSGIGLFNPNSVGNADTSNTRQNGKKTFLSSSEWRGVWLVAPLIAKLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQGDVKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMQTNNELFTTVLDMLGVLINGTLASDLSSASQGGPEENKRAYMNLVKKLKKELGDKRSDSIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGHKNPAPLSWAWFGTVRVDRKVIKYEEQQHLLLYHAHPKPKPRSYYLEPLPLPPEEEEEEEPTTPVSQEPERKSGDQDQGKHATDDEKKTKGRKRKSKSGSKADEYPQNNLYRVPPNYSPIPSQMMQHPQSALWGYTIMGQPQQPGFFVQNQPLPPGGSRLDPTSSFVPANTKQALSNMLQRRSGTVLQPPALHAVTPQQQLLQMKLLQQEQQQQRLLRHQAQARSLQQDVSSSAVSLQDGFDNIMGQPMDPAALFTPQVRPTPQLPQYPGLQQAQVLHCSTEACPTGTRCTAPRCLCSSSSSPAAWCSLLATPRGHTQPTPAPC

Target ORF information:

RefSeq Version	XM_015291836.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291836.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAACTGCTG 
TTGAAATCCT CCGACCTTGT TGGCAGCTAC ACCACCAGCT TGTGCCTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCT AAGGTGAAGC AAACGATATA CAGCAATGTT 
CAGCCTTCAA ATTCCAATCT GCTGTGGGAC CCGGAGTTCA TGCTTGATTT TATTGAGAAC 
CCTTCTGCTC ACAGCATTAA CTACTCCATG CTGGGCAAGA TCCTGAGTGA CAACGCAGCC 
AACCGCTACA GCTTTGTCTG CAATGCTCTA ATGAATGTGT GCATGGGGCA CCAAGATGCA 
GGGAGGATTA ATGACATAGC AAACCTTTGT GCAGAGCTGA CGGCGTGCTG CACCGTGCTG 
AGCTCGGAGT GGCTGGGGGT TGTCAAAGCT CTTTGCTGCT CTTCCAATCA CGTGTGGGGT 
TTCAATGATC TGCTCTGCAG CGTGGATGTG AGTGACCTGT CATTCCACGA TTCCCTGGCC 
ACTTTCATTG CCATACTGAT TGCCCGGCAG TGCTTTTCTC TGGAAGATGT CGTTCAGCAC 
GTAGCACTGC CTTCTCTTCT TGCAGCTGCC TGTGGGGACC CAGATGCTGA GCCTGGGGCC 
AGGATGACTT GTCGGCTTCT CCTGCACCTC TTCAGGACAC CCCAGGTCTG CCTCTTTCCC 
CAGGGAGCAG GAAAATTATT TCCTGGAATT CGTTCTTCTT GTGATCGTCA CCTCTTGGCT 
GCTGCTCACA ACAGCATAGA AGTGGGAGCT GTGTTTGCAG TCTTAAAAGC AATTTTGATG 
CTTGGTGACG CTGAGATCGG CAGCAGCAGT GTCACCCCTG TGAAGAACGA GGAGCTGCCC 
GTGAGGGGCT TGCTGGATGA GATCGCTGAG GATGAAATGT GGGCTTCCTC TCACGCTCTG 
AAATCACGTG GAAAAGCTGT TTCCATAGAA ACCGCCAGTT TGAGCGAGTA TGCTAGATAT 
GTGCTAAGGA CCATTTGCCA GCAGGAATGG GTTGGAGAGC ACTGTTTAAA AGAACCTGAG 
AGGCTGTGCA CAGATAAAGA TCTAATTTTG GATCCCGTTC TTTCAAACAA GCAAGCACAG 
AAACTTCTTC AGCTTATCTG TTATCCTCAT GGTGTGAAAG AATGCTCCGA GGGGGACAAC 
CCTCAGAGGC AGCACATCAA ACGCATACTT CAGAACTTAG ACCAGTGGAC TCTGCGGCAG 
TCTTGGTTGG AGCTGCAACT AATGATCAAG CAGTGCATGA AGGAGCCTGG TTCTGGGTCT 
GTGGCTGAAA TGAACAGCTT GTTGGATAAT ATTGCAAAGG CGACAATAGA GGTGTTTCAG 
CAATCCGCTG ATCTAAACAA TAACTCTTCT AACTCTGGTA TAGGCCTCTT CAATCCAAAC 
TCTGTTGGAA ATGCTGACAC AAGTAATACA AGACAGAATG GTAAAAAGAC ATTCCTAAGT 
TCCTCCGAGT GGCGAGGAGT GTGGCTGGTG GCTCCCTTGA TTGCAAAACT ACCTACCTCA 
GTTCAAGGTA GGGTCTTGAA AGCTGCGGGA GAGGAGCTGG AGAAAGGTCA GCATTTGGGG 
TCATCTTCTA AGAAGGAAAG AGATAGACAG AAGCAAAAGA GCATGTCTCT CTTGAGTCAG 
CAGCCTTTCC TTTCTTTGGT ACTTACTTGC CTTAAGGGAC AGGATGAGCA GCGGGAAGGG 
CTTCTAACAT CACTGCAGAA TCAAGTCAAT CAGATCCTTA GTAACTGGAG GGAAGAACGG 
TACCAGGGTG ATGTTAAAGC TAGGCAAATG ATGCATGAAG CCTTACAGCT TCGTCTGAAT 
TTGGTAGGTG GCATGTTTGA TACTGTGCAG AGGAGTACCC AGTGGACCAC AGACTGGGCA 
CTTCTGCTCC TCCAGATAAT CACTTCGGGA ACAGTTGATA TGCAGACCAA CAACGAATTG 
TTCACAACCG TGCTGGATAT GCTGGGTGTC CTCATCAACG GGACGCTCGC CTCCGACTTG 
TCCAGTGCTT CCCAAGGGGG ACCCGAGGAA AACAAAAGGG CTTACATGAA CTTAGTGAAA 
AAGTTAAAAA AGGAGCTGGG AGACAAGCGG TCAGACAGCA TTGACAAGGT TCGCCAGCTG 
CTCCCTTTGC CAAAGCAGAC ATGTGATATT ATTACCTGTG AGCCAATGGG ATCCTTGATT 
GACACCAAGG GCAACAAAAT TGCGGGGTTT GACTCCATTG ATAAGAAACA GGGTCTTCAG 
GTGTCAACAA AACAAAAGGT GTCCCCTTGG GATTTGTTTG AAGGTCATAA AAATCCTGCT 
CCTCTCTCCT GGGCCTGGTT TGGTACAGTT CGTGTCGACA GGAAGGTGAT CAAATACGAA 
GAGCAGCAGC ATCTGCTCCT GTACCATGCG CACCCCAAAC CCAAGCCGCG GAGCTACTAC 
CTCGAGCCCC TGCCTCTGCC TCCGGAGGAG GAGGAGGAGG AGGAGCCCAC CACCCCTGTC 
TCTCAGGAAC CAGAAAGGAA GTCAGGAGAT CAGGATCAGG GAAAGCATGC CACAGATGAC 
GAGAAGAAGA CAAAGGGCAG GAAGAGGAAG TCAAAGTCAG GCTCTAAGGC TGATGAATAT 
CCACAGAACA ACTTATACAG AGTGCCTCCC AATTACTCTC CCATTCCCTC ACAAATGATG 
CAGCATCCTC AGTCTGCCCT GTGGGGCTAC ACCATCATGG GGCAGCCGCA GCAGCCTGGC 
TTCTTCGTGC AGAACCAGCC CCTCCCTCCA GGTGGTTCCA GGCTGGATCC AACGAGCTCC 
TTCGTCCCAG CCAACACGAA GCAGGCCCTG TCCAACATGC TGCAGCGGCG CTCCGGCACC 
GTGCTGCAGC CCCCTGCCCT CCACGCAGTC ACACCTCAGC AGCAGTTGCT CCAGATGAAA 
CTCCTGCAGC AAGAGCAGCA GCAGCAGCGG CTCCTCAGGC ACCAAGCACA GGCACGGTCT 
CTCCAGCAGG ATGTCAGTAG CTCTGCCGTA TCCCTTCAGG ATGGCTTTGA CAACATAATG 
GGTCAGCCGA TGGACCCCGC TGCTCTTTTC ACCCCGCAAG TCCGACCCAC GCCACAGCTA 
CCGCAGTACC CAGGGCTTCA GCAAGCACAG GTACTGCACT GCTCCACAGA AGCATGCCCC 
ACGGGTACAC GATGTACAGC ACCCCGATGC CTTTGCAGCA GCAGCAGCAG CCCAGCAGCG 
TGGTGCTCTC TCCTGGCTAC ACCCCGCGGG CATACACAGC CCACTCCAGC CCCGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_015291828.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147314.1 Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X1, mRNA.

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CloneID	OGa40167
Clone ID Related Accession (Same CDS sequence)	XM_015291828.1
Accession Version	XM_015291828.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAACTGCTG 
TTGAAATCCT CCGACCTTGT TGGCAGCTAC ACCACCAGCT TGTGCCTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCT AAGGTGAAGC AAACGATATA CAGCAATGTT 
CAGCCTTCAA ATTCCAATCT GCTGTGGGAC CCGGAGTTCA TGCTTGATTT TATTGAGAAC 
CCTTCTGCTC ACAGCATTAA CTACTCCATG CTGGGCAAGA TCCTGAGTGA CAACGCAGCC 
AACCGCTACA GCTTTGTCTG CAATGCTCTA ATGAATGTGT GCATGGGGCA CCAAGATGCA 
GGGAGGATTA ATGACATAGC AAACCTTTGT GCAGAGCTGA CGGCGTGCTG CACCGTGCTG 
AGCTCGGAGT GGCTGGGGGT TGTCAAAGCT CTTTGCTGCT CTTCCAATCA CGTGTGGGGT 
TTCAATGATC TGCTCTGCAG CGTGGATGTG AGTGACCTGT CATTCCACGA TTCCCTGGCC 
ACTTTCATTG CCATACTGAT TGCCCGGCAG TGCTTTTCTC TGGAAGATGT CGTTCAGCAC 
GTAGCACTGC CTTCTCTTCT TGCAGCTGCC TGTGGGGACC CAGATGCTGA GCCTGGGGCC 
AGGATGACTT GTCGGCTTCT CCTGCACCTC TTCAGGACAC CCCAGGTCTG CCTCTTTCCC 
CAGGGAGCAG GAAAATTATT TCCTGGAATT CGTTCTTCTT GTGATCGTCA CCTCTTGGCT 
GCTGCTCACA ACAGCATAGA AGTGGGAGCT GTGTTTGCAG TCTTAAAAGC AATTTTGATG 
CTTGGTGACG CTGAGATCGG CAGCAGCAGT GTCACCCCTG TGAAGAACGA GGAGCTGCCC 
GTGAGGGGCT TGCTGGATGA GATCGCTGAG GATGAAATGT GGGCTTCCTC TCACGCTCTG 
AAATCACGTG GAAAAGCTGT TTCCATAGAA ACCGCCAGTT TGAGCGAGTA TGCTAGATAT 
GTGCTAAGGA CCATTTGCCA GCAGGAATGG GTTGGAGAGC ACTGTTTAAA AGAACCTGAG 
AGGCTGTGCA CAGATAAAGA TCTAATTTTG GATCCCGTTC TTTCAAACAA GCAAGCACAG 
AAACTTCTTC AGCTTATCTG TTATCCTCAT GGTGTGAAAG AATGCTCCGA GGGGGACAAC 
CCTCAGAGGC AGCACATCAA ACGCATACTT CAGAACTTAG ACCAGTGGAC TCTGCGGCAG 
TCTTGGTTGG AGCTGCAACT AATGATCAAG CAGTGCATGA AGGAGCCTGG TTCTGGGTCT 
GTGGCTGAAA TGAACAGCTT GTTGGATAAT ATTGCAAAGG CGACAATAGA GGTGTTTCAG 
CAATCCGCTG ATCTAAACAA TAACTCTTCT AACTCTGGTA TAGGCCTCTT CAATCCAAAC 
TCTGTTGGAA ATGCTGACAC AAGTAATACA AGACAGAATG GTAAAAAGAC ATTCCTAAGT 
TCCTCCGAGT GGCGAGGAGT GTGGCTGGTG GCTCCCTTGA TTGCAAAACT ACCTACCTCA 
GTTCAAGGTA GGGTCTTGAA AGCTGCGGGA GAGGAGCTGG AGAAAGGTCA GCATTTGGGG 
TCATCTTCTA AGAAGGAAAG AGATAGACAG AAGCAAAAGA GCATGTCTCT CTTGAGTCAG 
CAGCCTTTCC TTTCTTTGGT ACTTACTTGC CTTAAGGGAC AGGATGAGCA GCGGGAAGGG 
CTTCTAACAT CACTGCAGAA TCAAGTCAAT CAGATCCTTA GTAACTGGAG GGAAGAACGG 
TACCAGGGTG ATGTTAAAGC TAGGCAAATG ATGCATGAAG CCTTACAGCT TCGTCTGAAT 
TTGGTAGGTG GCATGTTTGA TACTGTGCAG AGGAGTACCC AGTGGACCAC AGACTGGGCA 
CTTCTGCTCC TCCAGATAAT CACTTCGGGA ACAGTTGATA TGCAGACCAA CAACGAATTG 
TTCACAACCG TGCTGGATAT GCTGGGTGTC CTCATCAACG GGACGCTCGC CTCCGACTTG 
TCCAGTGCTT CCCAAGGGGG ACCCGAGGAA AACAAAAGGG CTTACATGAA CTTAGTGAAA 
AAGTTAAAAA AGGAGCTGGG AGACAAGCGG TCAGACAGCA TTGACAAGGT TCGCCAGCTG 
CTCCCTTTGC CAAAGCAGAC ATGTGATATT ATTACCTGTG AGCCAATGGG ATCCTTGATT 
GACACCAAGG GCAACAAAAT TGCGGGGTTT GACTCCATTG ATAAGAAACA GGGTCTTCAG 
GTGTCAACAA AACAAAAGGT GTCCCCTTGG GATTTGTTTG AAGGTCATAA AAATCCTGCT 
CCTCTCTCCT GGGCCTGGTT TGGTACAGTT CGTGTCGACA GGAAGGTGAT CAAATACGAA 
GAGCAGCAGC ATCTGCTCCT GTACCATGCG CACCCCAAAC CCAAGCCGCG GAGCTACTAC 
CTCGAGCCCC TGCCTCTGCC TCCGGAGGAG GAGGAGGAGG AGGAGCCCAC CACCCCTGTC 
TCTCAGGAAC CAGAAAGGAA GTCAGGAGAT CAGGATCAGG GAAAGCATGC CACAGATGAC 
GAGAAGAAGA CAAAGGGCAG GAAGAGGAAG TCAAAGTCAG GCTCTAAGGC TGATGAATAT 
CCACAGAACA ACTTATACAG AGTGCCTCCC AATTACTCTC CCATTCCCTC ACAAATGATG 
CAGCATCCTC AGTCTGCCCT GTGGGGCTAC ACCATCATGG GGCAGCCGCA GCAGCCTGGC 
TTCTTCGTGC AGAACCAGCC CCTCCCTCCA GGTGGTTCCA GGCTGGATCC AACGAGCTCC 
TTCGTCCCAG CCAACACGAA GCAGGCCCTG TCCAACATGC TGCAGCGGCG CTCCGGCACC 
GTGCTGCAGC CCCCTGCCCT CCACGCAGTC ACACCTCAGC AGCAGTTGCT CCAGATGAAA 
CTCCTGCAGC AAGAGCAGCA GCAGCAGCGG CTCCTCAGGC ACCAAGCACA GGCACGGTCT 
CTCCAGCAGG ATGTCAGTAG CTCTGCCGTA TCCCTTCAGG ATGGCTTTGA CAACATAATG 
GGTCAGCCGA TGGACCCCGC TGCTCTTTTC ACCCCGCAAG TCCGACCCAC GCCACAGCTA 
CCGCAGTACC CAGGGCTTCA GCAAGCACAG AGCATGCCCC ACGGGTACAC GATGTACAGC 
ACCCCGATGC CTTTGCAGCA GCAGCAGCAG CCCAGCAGCG TGGTGCTCTC TCCTGGCTAC 
ACCCCGCGGG CATACACAGC CCACTCCAGC CCCGTGCTGA TGGAGCGGCT GCGGCCGGGG 
CAGCCGGGCA GCGTGGCTCA GCAGCAGGCA GCTGCCTACA TGCAGCCCCT GGCGGGTGCT 
CAGCGGTTGA CCCATCAGCC TCTGCAGCCA AACTCATTGA TAGGGGCAGG CCTGGACAGT 
GCATCAACCG CACGTACACA TCCAACCTTG AGCTCAGCAC AGCTGCCCCA GGAGCAAATG 
CGACAGAGGC AGCAGGAGAT CCGACAGCAG AGACTCTTCC AGATGCAGCA GGGGCAGCAG 
CAGCAGACTC TTGATCTTCA GCCTGTGCAG CCACAGCAGC CCCTGGTAAG TGGGATGGAT 
GGTGAGCGTG GGGCAGGGGC GTGGGATGAG CGGGTGAAGC TTACAGTGAA TTTGCAGTGC 
TGTTGTCCAT GTTTCCTGGA CATGGCCATT TTTTCCAGAT ATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147314.1
CDS	142..6669
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPRQKEDELTAVNVKQGFSNQPAFSGDEHGSARNIVINASKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHNWFTDLAGSKPLTILAKKVPILSKKEDVFAYLAKYSVPLLRAAWLIKMTCAYYAAISEAKIKKRQATDPNIEWTQIITRYLREQLAKVAEFYHVTSSQGNSSVVMPQEMEQALKQWEYNEKLSFYMFQEGMLERHEYLTWILDVLEKIRPTDDDILKLVLPLMLQYSEEFVQSAYLSRRLAYFCARRLSLLLSDGPSLAAAHSPHVIIAPSNPPLAVPSPTAPGPVLSPVQLTCSDFLSCPQHRPLVYGLSCMLQTITLCCPSALVWNYSTNENKNMNPGSPLDLLQVAPSSLPMPGGNSTFNQQVRAKIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRSDSSNSMETLYHKIFWANQNKDNQEAAPSDEAVVMLLCEWAVSCKRSGKHRAMVVAKLLEKRQAEIEAERCGEAEVLDEKESLSSASLTSSSLPVFQNILLRFLDTQAPSLSDPNSDHEKTEFVNLVLLFSEFTRHDVFSHDAYMCTLISRGDLSITATTRPRSPNGETGDEHYSKDHDMKMEDHNIMEHMGTDSGPTSIFDDVDKGDFKADFGSEFPIFSPMPGESCENMNSSLDRRISVASEKSVKRERLREVIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRMILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQEAFPTLETVFTKLQQLSYFDQHQVTSQISSNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRRYHACLILNSEQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYSNVQPSNSNLLWDPEFMLDFIENPSAHSINYSMLGKILSDNAANRYSFVCNALMNVCMGHQDAGRINDIANLCAELTACCTVLSSEWLGVVKALCCSSNHVWGFNDLLCSVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDPDAEPGARMTCRLLLHLFRTPQVCLFPQGAGKLFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAILMLGDAEIGSSSVTPVKNEELPVRGLLDEIAEDEMWASSHALKSRGKAVSIETASLSEYARYVLRTICQQEWVGEHCLKEPERLCTDKDLILDPVLSNKQAQKLLQLICYPHGVKECSEGDNPQRQHIKRILQNLDQWTLRQSWLELQLMIKQCMKEPGSGSVAEMNSLLDNIAKATIEVFQQSADLNNNSSNSGIGLFNPNSVGNADTSNTRQNGKKTFLSSSEWRGVWLVAPLIAKLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQGDVKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMQTNNELFTTVLDMLGVLINGTLASDLSSASQGGPEENKRAYMNLVKKLKKELGDKRSDSIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGHKNPAPLSWAWFGTVRVDRKVIKYEEQQHLLLYHAHPKPKPRSYYLEPLPLPPEEEEEEEPTTPVSQEPERKSGDQDQGKHATDDEKKTKGRKRKSKSGSKADEYPQNNLYRVPPNYSPIPSQMMQHPQSALWGYTIMGQPQQPGFFVQNQPLPPGGSRLDPTSSFVPANTKQALSNMLQRRSGTVLQPPALHAVTPQQQLLQMKLLQQEQQQQRLLRHQAQARSLQQDVSSSAVSLQDGFDNIMGQPMDPAALFTPQVRPTPQLPQYPGLQQAQSMPHGYTMYSTPMPLQQQQQPSSVVLSPGYTPRAYTAHSSPVLMERLRPGQPGSVAQQQAAAYMQPLAGAQRLTHQPLQPNSLIGAGLDSASTARTHPTLSSAQLPQEQMRQRQQEIRQQRLFQMQQGQQQQTLDLQPVQPQQPLVSGMDGERGAGAWDERVKLTVNLQCCCPCFLDMAIFSRYV

Target ORF information:

RefSeq Version	XM_015291828.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291828.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAACTGCTG 
TTGAAATCCT CCGACCTTGT TGGCAGCTAC ACCACCAGCT TGTGCCTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCT AAGGTGAAGC AAACGATATA CAGCAATGTT 
CAGCCTTCAA ATTCCAATCT GCTGTGGGAC CCGGAGTTCA TGCTTGATTT TATTGAGAAC 
CCTTCTGCTC ACAGCATTAA CTACTCCATG CTGGGCAAGA TCCTGAGTGA CAACGCAGCC 
AACCGCTACA GCTTTGTCTG CAATGCTCTA ATGAATGTGT GCATGGGGCA CCAAGATGCA 
GGGAGGATTA ATGACATAGC AAACCTTTGT GCAGAGCTGA CGGCGTGCTG CACCGTGCTG 
AGCTCGGAGT GGCTGGGGGT TGTCAAAGCT CTTTGCTGCT CTTCCAATCA CGTGTGGGGT 
TTCAATGATC TGCTCTGCAG CGTGGATGTG AGTGACCTGT CATTCCACGA TTCCCTGGCC 
ACTTTCATTG CCATACTGAT TGCCCGGCAG TGCTTTTCTC TGGAAGATGT CGTTCAGCAC 
GTAGCACTGC CTTCTCTTCT TGCAGCTGCC TGTGGGGACC CAGATGCTGA GCCTGGGGCC 
AGGATGACTT GTCGGCTTCT CCTGCACCTC TTCAGGACAC CCCAGGTCTG CCTCTTTCCC 
CAGGGAGCAG GAAAATTATT TCCTGGAATT CGTTCTTCTT GTGATCGTCA CCTCTTGGCT 
GCTGCTCACA ACAGCATAGA AGTGGGAGCT GTGTTTGCAG TCTTAAAAGC AATTTTGATG 
CTTGGTGACG CTGAGATCGG CAGCAGCAGT GTCACCCCTG TGAAGAACGA GGAGCTGCCC 
GTGAGGGGCT TGCTGGATGA GATCGCTGAG GATGAAATGT GGGCTTCCTC TCACGCTCTG 
AAATCACGTG GAAAAGCTGT TTCCATAGAA ACCGCCAGTT TGAGCGAGTA TGCTAGATAT 
GTGCTAAGGA CCATTTGCCA GCAGGAATGG GTTGGAGAGC ACTGTTTAAA AGAACCTGAG 
AGGCTGTGCA CAGATAAAGA TCTAATTTTG GATCCCGTTC TTTCAAACAA GCAAGCACAG 
AAACTTCTTC AGCTTATCTG TTATCCTCAT GGTGTGAAAG AATGCTCCGA GGGGGACAAC 
CCTCAGAGGC AGCACATCAA ACGCATACTT CAGAACTTAG ACCAGTGGAC TCTGCGGCAG 
TCTTGGTTGG AGCTGCAACT AATGATCAAG CAGTGCATGA AGGAGCCTGG TTCTGGGTCT 
GTGGCTGAAA TGAACAGCTT GTTGGATAAT ATTGCAAAGG CGACAATAGA GGTGTTTCAG 
CAATCCGCTG ATCTAAACAA TAACTCTTCT AACTCTGGTA TAGGCCTCTT CAATCCAAAC 
TCTGTTGGAA ATGCTGACAC AAGTAATACA AGACAGAATG GTAAAAAGAC ATTCCTAAGT 
TCCTCCGAGT GGCGAGGAGT GTGGCTGGTG GCTCCCTTGA TTGCAAAACT ACCTACCTCA 
GTTCAAGGTA GGGTCTTGAA AGCTGCGGGA GAGGAGCTGG AGAAAGGTCA GCATTTGGGG 
TCATCTTCTA AGAAGGAAAG AGATAGACAG AAGCAAAAGA GCATGTCTCT CTTGAGTCAG 
CAGCCTTTCC TTTCTTTGGT ACTTACTTGC CTTAAGGGAC AGGATGAGCA GCGGGAAGGG 
CTTCTAACAT CACTGCAGAA TCAAGTCAAT CAGATCCTTA GTAACTGGAG GGAAGAACGG 
TACCAGGGTG ATGTTAAAGC TAGGCAAATG ATGCATGAAG CCTTACAGCT TCGTCTGAAT 
TTGGTAGGTG GCATGTTTGA TACTGTGCAG AGGAGTACCC AGTGGACCAC AGACTGGGCA 
CTTCTGCTCC TCCAGATAAT CACTTCGGGA ACAGTTGATA TGCAGACCAA CAACGAATTG 
TTCACAACCG TGCTGGATAT GCTGGGTGTC CTCATCAACG GGACGCTCGC CTCCGACTTG 
TCCAGTGCTT CCCAAGGGGG ACCCGAGGAA AACAAAAGGG CTTACATGAA CTTAGTGAAA 
AAGTTAAAAA AGGAGCTGGG AGACAAGCGG TCAGACAGCA TTGACAAGGT TCGCCAGCTG 
CTCCCTTTGC CAAAGCAGAC ATGTGATATT ATTACCTGTG AGCCAATGGG ATCCTTGATT 
GACACCAAGG GCAACAAAAT TGCGGGGTTT GACTCCATTG ATAAGAAACA GGGTCTTCAG 
GTGTCAACAA AACAAAAGGT GTCCCCTTGG GATTTGTTTG AAGGTCATAA AAATCCTGCT 
CCTCTCTCCT GGGCCTGGTT TGGTACAGTT CGTGTCGACA GGAAGGTGAT CAAATACGAA 
GAGCAGCAGC ATCTGCTCCT GTACCATGCG CACCCCAAAC CCAAGCCGCG GAGCTACTAC 
CTCGAGCCCC TGCCTCTGCC TCCGGAGGAG GAGGAGGAGG AGGAGCCCAC CACCCCTGTC 
TCTCAGGAAC CAGAAAGGAA GTCAGGAGAT CAGGATCAGG GAAAGCATGC CACAGATGAC 
GAGAAGAAGA CAAAGGGCAG GAAGAGGAAG TCAAAGTCAG GCTCTAAGGC TGATGAATAT 
CCACAGAACA ACTTATACAG AGTGCCTCCC AATTACTCTC CCATTCCCTC ACAAATGATG 
CAGCATCCTC AGTCTGCCCT GTGGGGCTAC ACCATCATGG GGCAGCCGCA GCAGCCTGGC 
TTCTTCGTGC AGAACCAGCC CCTCCCTCCA GGTGGTTCCA GGCTGGATCC AACGAGCTCC 
TTCGTCCCAG CCAACACGAA GCAGGCCCTG TCCAACATGC TGCAGCGGCG CTCCGGCACC 
GTGCTGCAGC CCCCTGCCCT CCACGCAGTC ACACCTCAGC AGCAGTTGCT CCAGATGAAA 
CTCCTGCAGC AAGAGCAGCA GCAGCAGCGG CTCCTCAGGC ACCAAGCACA GGCACGGTCT 
CTCCAGCAGG ATGTCAGTAG CTCTGCCGTA TCCCTTCAGG ATGGCTTTGA CAACATAATG 
GGTCAGCCGA TGGACCCCGC TGCTCTTTTC ACCCCGCAAG TCCGACCCAC GCCACAGCTA 
CCGCAGTACC CAGGGCTTCA GCAAGCACAG AGCATGCCCC ACGGGTACAC GATGTACAGC 
ACCCCGATGC CTTTGCAGCA GCAGCAGCAG CCCAGCAGCG TGGTGCTCTC TCCTGGCTAC 
ACCCCGCGGG CATACACAGC CCACTCCAGC CCCGTGCTGA TGGAGCGGCT GCGGCCGGGG 
CAGCCGGGCA GCGTGGCTCA GCAGCAGGCA GCTGCCTACA TGCAGCCCCT GGCGGGTGCT 
CAGCGGTTGA CCCATCAGCC TCTGCAGCCA AACTCATTGA TAGGGGCAGG CCTGGACAGT 
GCATCAACCG CACGTACACA TCCAACCTTG AGCTCAGCAC AGCTGCCCCA GGAGCAAATG 
CGACAGAGGC AGCAGGAGAT CCGACAGCAG AGACTCTTCC AGATGCAGCA GGGGCAGCAG 
CAGCAGACTC TTGATCTTCA GCCTGTGCAG CCACAGCAGC CCCTGGTAAG TGGGATGGAT 
GGTGAGCGTG GGGCAGGGGC GTGGGATGAG CGGGTGAAGC TTACAGTGAA TTTGCAGTGC 
TGTTGTCCAT GTTTCCTGGA CATGGCCATT TTTTCCAGAT ATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_015291829.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147315.1 Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X2, mRNA.

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CloneID	OGa40168
Clone ID Related Accession (Same CDS sequence)	XM_015291829.1
Accession Version	XM_015291829.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAACTGCTG 
TTGAAATCCT CCGACCTTGT TGGCAGCTAC ACCACCAGCT TGTGCCTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTGC CTGTTCTAAG GTGAAGCAAA CGATATACAG CAATGTTCAG 
CCTTCAAATT CCAATCTGCT GTGGGACCCG GAGTTCATGC TTGATTTTAT TGAGAACCCT 
TCTGCTCACA GCATTAACTA CTCCATGCTG GGCAAGATCC TGAGTGACAA CGCAGCCAAC 
CGCTACAGCT TTGTCTGCAA TGCTCTAATG AATGTGTGCA TGGGGCACCA AGATGCAGGG 
AGGATTAATG ACATAGCAAA CCTTTGTGCA GAGCTGACGG CGTGCTGCAC CGTGCTGAGC 
TCGGAGTGGC TGGGGGTTGT CAAAGCTCTT TGCTGCTCTT CCAATCACGT GTGGGGTTTC 
AATGATCTGC TCTGCAGCGT GGATGTGAGT GACCTGTCAT TCCACGATTC CCTGGCCACT 
TTCATTGCCA TACTGATTGC CCGGCAGTGC TTTTCTCTGG AAGATGTCGT TCAGCACGTA 
GCACTGCCTT CTCTTCTTGC AGCTGCCTGT GGGGACCCAG ATGCTGAGCC TGGGGCCAGG 
ATGACTTGTC GGCTTCTCCT GCACCTCTTC AGGACACCCC AGGTCTGCCT CTTTCCCCAG 
GGAGCAGGAA AATTATTTCC TGGAATTCGT TCTTCTTGTG ATCGTCACCT CTTGGCTGCT 
GCTCACAACA GCATAGAAGT GGGAGCTGTG TTTGCAGTCT TAAAAGCAAT TTTGATGCTT 
GGTGACGCTG AGATCGGCAG CAGCAGTGTC ACCCCTGTGA AGAACGAGGA GCTGCCCGTG 
AGGGGCTTGC TGGATGAGAT CGCTGAGGAT GAAATGTGGG CTTCCTCTCA CGCTCTGAAA 
TCACGTGGAA AAGCTGTTTC CATAGAAACC GCCAGTTTGA GCGAGTATGC TAGATATGTG 
CTAAGGACCA TTTGCCAGCA GGAATGGGTT GGAGAGCACT GTTTAAAAGA ACCTGAGAGG 
CTGTGCACAG ATAAAGATCT AATTTTGGAT CCCGTTCTTT CAAACAAGCA AGCACAGAAA 
CTTCTTCAGC TTATCTGTTA TCCTCATGGT GTGAAAGAAT GCTCCGAGGG GGACAACCCT 
CAGAGGCAGC ACATCAAACG CATACTTCAG AACTTAGACC AGTGGACTCT GCGGCAGTCT 
TGGTTGGAGC TGCAACTAAT GATCAAGCAG TGCATGAAGG AGCCTGGTTC TGGGTCTGTG 
GCTGAAATGA ACAGCTTGTT GGATAATATT GCAAAGGCGA CAATAGAGGT GTTTCAGCAA 
TCCGCTGATC TAAACAATAA CTCTTCTAAC TCTGGTATAG GCCTCTTCAA TCCAAACTCT 
GTTGGAAATG CTGACACAAG TAATACAAGA CAGAATGGTA AAAAGACATT CCTAAGTTCC 
TCCGAGTGGC GAGGAGTGTG GCTGGTGGCT CCCTTGATTG CAAAACTACC TACCTCAGTT 
CAAGGTAGGG TCTTGAAAGC TGCGGGAGAG GAGCTGGAGA AAGGTCAGCA TTTGGGGTCA 
TCTTCTAAGA AGGAAAGAGA TAGACAGAAG CAAAAGAGCA TGTCTCTCTT GAGTCAGCAG 
CCTTTCCTTT CTTTGGTACT TACTTGCCTT AAGGGACAGG ATGAGCAGCG GGAAGGGCTT 
CTAACATCAC TGCAGAATCA AGTCAATCAG ATCCTTAGTA ACTGGAGGGA AGAACGGTAC 
CAGGGTGATG TTAAAGCTAG GCAAATGATG CATGAAGCCT TACAGCTTCG TCTGAATTTG 
GTAGGTGGCA TGTTTGATAC TGTGCAGAGG AGTACCCAGT GGACCACAGA CTGGGCACTT 
CTGCTCCTCC AGATAATCAC TTCGGGAACA GTTGATATGC AGACCAACAA CGAATTGTTC 
ACAACCGTGC TGGATATGCT GGGTGTCCTC ATCAACGGGA CGCTCGCCTC CGACTTGTCC 
AGTGCTTCCC AAGGGGGACC CGAGGAAAAC AAAAGGGCTT ACATGAACTT AGTGAAAAAG 
TTAAAAAAGG AGCTGGGAGA CAAGCGGTCA GACAGCATTG ACAAGGTTCG CCAGCTGCTC 
CCTTTGCCAA AGCAGACATG TGATATTATT ACCTGTGAGC CAATGGGATC CTTGATTGAC 
ACCAAGGGCA ACAAAATTGC GGGGTTTGAC TCCATTGATA AGAAACAGGG TCTTCAGGTG 
TCAACAAAAC AAAAGGTGTC CCCTTGGGAT TTGTTTGAAG GTCATAAAAA TCCTGCTCCT 
CTCTCCTGGG CCTGGTTTGG TACAGTTCGT GTCGACAGGA AGGTGATCAA ATACGAAGAG 
CAGCAGCATC TGCTCCTGTA CCATGCGCAC CCCAAACCCA AGCCGCGGAG CTACTACCTC 
GAGCCCCTGC CTCTGCCTCC GGAGGAGGAG GAGGAGGAGG AGCCCACCAC CCCTGTCTCT 
CAGGAACCAG AAAGGAAGTC AGGAGATCAG GATCAGGGAA AGCATGCCAC AGATGACGAG 
AAGAAGACAA AGGGCAGGAA GAGGAAGTCA AAGTCAGGCT CTAAGGCTGA TGAATATCCA 
CAGAACAACT TATACAGAGT GCCTCCCAAT TACTCTCCCA TTCCCTCACA AATGATGCAG 
CATCCTCAGT CTGCCCTGTG GGGCTACACC ATCATGGGGC AGCCGCAGCA GCCTGGCTTC 
TTCGTGCAGA ACCAGCCCCT CCCTCCAGGT GGTTCCAGGC TGGATCCAAC GAGCTCCTTC 
GTCCCAGCCA ACACGAAGCA GGCCCTGTCC AACATGCTGC AGCGGCGCTC CGGCACCGTG 
CTGCAGCCCC CTGCCCTCCA CGCAGTCACA CCTCAGCAGC AGTTGCTCCA GATGAAACTC 
CTGCAGCAAG AGCAGCAGCA GCAGCGGCTC CTCAGGCACC AAGCACAGGC ACGGTCTCTC 
CAGCAGGATG TCAGTAGCTC TGCCGTATCC CTTCAGGATG GCTTTGACAA CATAATGGGT 
CAGCCGATGG ACCCCGCTGC TCTTTTCACC CCGCAAGTCC GACCCACGCC ACAGCTACCG 
CAGTACCCAG GGCTTCAGCA AGCACAGAGC ATGCCCCACG GGTACACGAT GTACAGCACC 
CCGATGCCTT TGCAGCAGCA GCAGCAGCCC AGCAGCGTGG TGCTCTCTCC TGGCTACACC 
CCGCGGGCAT ACACAGCCCA CTCCAGCCCC GTGCTGATGG AGCGGCTGCG GCCGGGGCAG 
CCGGGCAGCG TGGCTCAGCA GCAGGCAGCT GCCTACATGC AGCCCCTGGC GGGTGCTCAG 
CGGTTGACCC ATCAGCCTCT GCAGCCAAAC TCATTGATAG GGGCAGGCCT GGACAGTGCA 
TCAACCGCAC GTACACATCC AACCTTGAGC TCAGCACAGC TGCCCCAGGA GCAAATGCGA 
CAGAGGCAGC AGGAGATCCG ACAGCAGAGA CTCTTCCAGA TGCAGCAGGG GCAGCAGCAG 
CAGACTCTTG ATCTTCAGCC TGTGCAGCCA CAGCAGCCCC TGGTAAGTGG GATGGATGGT 
GAGCGTGGGG CAGGGGCGTG GGATGAGCGG GTGAAGCTTA CAGTGAATTT GCAGTGCTGT 
TGTCCATGTT TCCTGGACAT GGCCATTTTT TCCAGATATG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147315.1
CDS	142..6666
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPRQKEDELTAVNVKQGFSNQPAFSGDEHGSARNIVINASKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHNWFTDLAGSKPLTILAKKVPILSKKEDVFAYLAKYSVPLLRAAWLIKMTCAYYAAISEAKIKKRQATDPNIEWTQIITRYLREQLAKVAEFYHVTSSQGNSSVVMPQEMEQALKQWEYNEKLSFYMFQEGMLERHEYLTWILDVLEKIRPTDDDILKLVLPLMLQYSEEFVQSAYLSRRLAYFCARRLSLLLSDGPSLAAAHSPHVIIAPSNPPLAVPSPTAPGPVLSPVQLTCSDFLSCPQHRPLVYGLSCMLQTITLCCPSALVWNYSTNENKNMNPGSPLDLLQVAPSSLPMPGGNSTFNQQVRAKIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRSDSSNSMETLYHKIFWANQNKDNQEAAPSDEAVVMLLCEWAVSCKRSGKHRAMVVAKLLEKRQAEIEAERCGEAEVLDEKESLSSASLTSSSLPVFQNILLRFLDTQAPSLSDPNSDHEKTEFVNLVLLFSEFTRHDVFSHDAYMCTLISRGDLSITATTRPRSPNGETGDEHYSKDHDMKMEDHNIMEHMGTDSGPTSIFDDVDKGDFKADFGSEFPIFSPMPGESCENMNSSLDRRISVASEKSVKRERLREVIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRMILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQEAFPTLETVFTKLQQLSYFDQHQVTSQISSNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRRYHACLILNSEQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSACSKVKQTIYSNVQPSNSNLLWDPEFMLDFIENPSAHSINYSMLGKILSDNAANRYSFVCNALMNVCMGHQDAGRINDIANLCAELTACCTVLSSEWLGVVKALCCSSNHVWGFNDLLCSVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDPDAEPGARMTCRLLLHLFRTPQVCLFPQGAGKLFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAILMLGDAEIGSSSVTPVKNEELPVRGLLDEIAEDEMWASSHALKSRGKAVSIETASLSEYARYVLRTICQQEWVGEHCLKEPERLCTDKDLILDPVLSNKQAQKLLQLICYPHGVKECSEGDNPQRQHIKRILQNLDQWTLRQSWLELQLMIKQCMKEPGSGSVAEMNSLLDNIAKATIEVFQQSADLNNNSSNSGIGLFNPNSVGNADTSNTRQNGKKTFLSSSEWRGVWLVAPLIAKLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQGDVKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMQTNNELFTTVLDMLGVLINGTLASDLSSASQGGPEENKRAYMNLVKKLKKELGDKRSDSIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGHKNPAPLSWAWFGTVRVDRKVIKYEEQQHLLLYHAHPKPKPRSYYLEPLPLPPEEEEEEEPTTPVSQEPERKSGDQDQGKHATDDEKKTKGRKRKSKSGSKADEYPQNNLYRVPPNYSPIPSQMMQHPQSALWGYTIMGQPQQPGFFVQNQPLPPGGSRLDPTSSFVPANTKQALSNMLQRRSGTVLQPPALHAVTPQQQLLQMKLLQQEQQQQRLLRHQAQARSLQQDVSSSAVSLQDGFDNIMGQPMDPAALFTPQVRPTPQLPQYPGLQQAQSMPHGYTMYSTPMPLQQQQQPSSVVLSPGYTPRAYTAHSSPVLMERLRPGQPGSVAQQQAAAYMQPLAGAQRLTHQPLQPNSLIGAGLDSASTARTHPTLSSAQLPQEQMRQRQQEIRQQRLFQMQQGQQQQTLDLQPVQPQQPLVSGMDGERGAGAWDERVKLTVNLQCCCPCFLDMAIFSRYV

Target ORF information:

RefSeq Version	XM_015291829.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291829.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAACTGCTG 
TTGAAATCCT CCGACCTTGT TGGCAGCTAC ACCACCAGCT TGTGCCTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTGC CTGTTCTAAG GTGAAGCAAA CGATATACAG CAATGTTCAG 
CCTTCAAATT CCAATCTGCT GTGGGACCCG GAGTTCATGC TTGATTTTAT TGAGAACCCT 
TCTGCTCACA GCATTAACTA CTCCATGCTG GGCAAGATCC TGAGTGACAA CGCAGCCAAC 
CGCTACAGCT TTGTCTGCAA TGCTCTAATG AATGTGTGCA TGGGGCACCA AGATGCAGGG 
AGGATTAATG ACATAGCAAA CCTTTGTGCA GAGCTGACGG CGTGCTGCAC CGTGCTGAGC 
TCGGAGTGGC TGGGGGTTGT CAAAGCTCTT TGCTGCTCTT CCAATCACGT GTGGGGTTTC 
AATGATCTGC TCTGCAGCGT GGATGTGAGT GACCTGTCAT TCCACGATTC CCTGGCCACT 
TTCATTGCCA TACTGATTGC CCGGCAGTGC TTTTCTCTGG AAGATGTCGT TCAGCACGTA 
GCACTGCCTT CTCTTCTTGC AGCTGCCTGT GGGGACCCAG ATGCTGAGCC TGGGGCCAGG 
ATGACTTGTC GGCTTCTCCT GCACCTCTTC AGGACACCCC AGGTCTGCCT CTTTCCCCAG 
GGAGCAGGAA AATTATTTCC TGGAATTCGT TCTTCTTGTG ATCGTCACCT CTTGGCTGCT 
GCTCACAACA GCATAGAAGT GGGAGCTGTG TTTGCAGTCT TAAAAGCAAT TTTGATGCTT 
GGTGACGCTG AGATCGGCAG CAGCAGTGTC ACCCCTGTGA AGAACGAGGA GCTGCCCGTG 
AGGGGCTTGC TGGATGAGAT CGCTGAGGAT GAAATGTGGG CTTCCTCTCA CGCTCTGAAA 
TCACGTGGAA AAGCTGTTTC CATAGAAACC GCCAGTTTGA GCGAGTATGC TAGATATGTG 
CTAAGGACCA TTTGCCAGCA GGAATGGGTT GGAGAGCACT GTTTAAAAGA ACCTGAGAGG 
CTGTGCACAG ATAAAGATCT AATTTTGGAT CCCGTTCTTT CAAACAAGCA AGCACAGAAA 
CTTCTTCAGC TTATCTGTTA TCCTCATGGT GTGAAAGAAT GCTCCGAGGG GGACAACCCT 
CAGAGGCAGC ACATCAAACG CATACTTCAG AACTTAGACC AGTGGACTCT GCGGCAGTCT 
TGGTTGGAGC TGCAACTAAT GATCAAGCAG TGCATGAAGG AGCCTGGTTC TGGGTCTGTG 
GCTGAAATGA ACAGCTTGTT GGATAATATT GCAAAGGCGA CAATAGAGGT GTTTCAGCAA 
TCCGCTGATC TAAACAATAA CTCTTCTAAC TCTGGTATAG GCCTCTTCAA TCCAAACTCT 
GTTGGAAATG CTGACACAAG TAATACAAGA CAGAATGGTA AAAAGACATT CCTAAGTTCC 
TCCGAGTGGC GAGGAGTGTG GCTGGTGGCT CCCTTGATTG CAAAACTACC TACCTCAGTT 
CAAGGTAGGG TCTTGAAAGC TGCGGGAGAG GAGCTGGAGA AAGGTCAGCA TTTGGGGTCA 
TCTTCTAAGA AGGAAAGAGA TAGACAGAAG CAAAAGAGCA TGTCTCTCTT GAGTCAGCAG 
CCTTTCCTTT CTTTGGTACT TACTTGCCTT AAGGGACAGG ATGAGCAGCG GGAAGGGCTT 
CTAACATCAC TGCAGAATCA AGTCAATCAG ATCCTTAGTA ACTGGAGGGA AGAACGGTAC 
CAGGGTGATG TTAAAGCTAG GCAAATGATG CATGAAGCCT TACAGCTTCG TCTGAATTTG 
GTAGGTGGCA TGTTTGATAC TGTGCAGAGG AGTACCCAGT GGACCACAGA CTGGGCACTT 
CTGCTCCTCC AGATAATCAC TTCGGGAACA GTTGATATGC AGACCAACAA CGAATTGTTC 
ACAACCGTGC TGGATATGCT GGGTGTCCTC ATCAACGGGA CGCTCGCCTC CGACTTGTCC 
AGTGCTTCCC AAGGGGGACC CGAGGAAAAC AAAAGGGCTT ACATGAACTT AGTGAAAAAG 
TTAAAAAAGG AGCTGGGAGA CAAGCGGTCA GACAGCATTG ACAAGGTTCG CCAGCTGCTC 
CCTTTGCCAA AGCAGACATG TGATATTATT ACCTGTGAGC CAATGGGATC CTTGATTGAC 
ACCAAGGGCA ACAAAATTGC GGGGTTTGAC TCCATTGATA AGAAACAGGG TCTTCAGGTG 
TCAACAAAAC AAAAGGTGTC CCCTTGGGAT TTGTTTGAAG GTCATAAAAA TCCTGCTCCT 
CTCTCCTGGG CCTGGTTTGG TACAGTTCGT GTCGACAGGA AGGTGATCAA ATACGAAGAG 
CAGCAGCATC TGCTCCTGTA CCATGCGCAC CCCAAACCCA AGCCGCGGAG CTACTACCTC 
GAGCCCCTGC CTCTGCCTCC GGAGGAGGAG GAGGAGGAGG AGCCCACCAC CCCTGTCTCT 
CAGGAACCAG AAAGGAAGTC AGGAGATCAG GATCAGGGAA AGCATGCCAC AGATGACGAG 
AAGAAGACAA AGGGCAGGAA GAGGAAGTCA AAGTCAGGCT CTAAGGCTGA TGAATATCCA 
CAGAACAACT TATACAGAGT GCCTCCCAAT TACTCTCCCA TTCCCTCACA AATGATGCAG 
CATCCTCAGT CTGCCCTGTG GGGCTACACC ATCATGGGGC AGCCGCAGCA GCCTGGCTTC 
TTCGTGCAGA ACCAGCCCCT CCCTCCAGGT GGTTCCAGGC TGGATCCAAC GAGCTCCTTC 
GTCCCAGCCA ACACGAAGCA GGCCCTGTCC AACATGCTGC AGCGGCGCTC CGGCACCGTG 
CTGCAGCCCC CTGCCCTCCA CGCAGTCACA CCTCAGCAGC AGTTGCTCCA GATGAAACTC 
CTGCAGCAAG AGCAGCAGCA GCAGCGGCTC CTCAGGCACC AAGCACAGGC ACGGTCTCTC 
CAGCAGGATG TCAGTAGCTC TGCCGTATCC CTTCAGGATG GCTTTGACAA CATAATGGGT 
CAGCCGATGG ACCCCGCTGC TCTTTTCACC CCGCAAGTCC GACCCACGCC ACAGCTACCG 
CAGTACCCAG GGCTTCAGCA AGCACAGAGC ATGCCCCACG GGTACACGAT GTACAGCACC 
CCGATGCCTT TGCAGCAGCA GCAGCAGCCC AGCAGCGTGG TGCTCTCTCC TGGCTACACC 
CCGCGGGCAT ACACAGCCCA CTCCAGCCCC GTGCTGATGG AGCGGCTGCG GCCGGGGCAG 
CCGGGCAGCG TGGCTCAGCA GCAGGCAGCT GCCTACATGC AGCCCCTGGC GGGTGCTCAG 
CGGTTGACCC ATCAGCCTCT GCAGCCAAAC TCATTGATAG GGGCAGGCCT GGACAGTGCA 
TCAACCGCAC GTACACATCC AACCTTGAGC TCAGCACAGC TGCCCCAGGA GCAAATGCGA 
CAGAGGCAGC AGGAGATCCG ACAGCAGAGA CTCTTCCAGA TGCAGCAGGG GCAGCAGCAG 
CAGACTCTTG ATCTTCAGCC TGTGCAGCCA CAGCAGCCCC TGGTAAGTGG GATGGATGGT 
GAGCGTGGGG CAGGGGCGTG GGATGAGCGG GTGAAGCTTA CAGTGAATTT GCAGTGCTGT 
TGTCCATGTT TCCTGGACAT GGCCATTTTT TCCAGATATG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_015291830.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147316.1 Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X3, mRNA.

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CloneID	OGa40169
Clone ID Related Accession (Same CDS sequence)	XM_015291830.1
Accession Version	XM_015291830.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAACTGCTG 
TTGAAATCCT CCGACCTTGT TGGCAGCTAC ACCACCAGCT TGTGCCTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCT AAGGTGAAGC AAACGATATA CAGCAATGTT 
CAGCCTTCAA ATTCCAATCT GCTGTGGGAC CCGGAGTTCA TGCTTGATTT TATTGAGAAC 
CCTTCTGCTC ACAGCATTAA CTACTCCATG CTGGGCAAGA TCCTGAGTGA CAACGCAGCC 
AACCGCTACA GCTTTGTCTG CAATGCTCTA ATGAATGTGT GCATGGGGCA CCAAGATGCA 
GGGAGGATTA ATGACATAGC AAACCTTTGT GCAGAGCTGA CGGCGTGCTG CACCGTGCTG 
AGCTCGGAGT GGCTGGGGGT TGTCAAAGCT CTTTGCTGCT CTTCCAATCA CGTGTGGGGT 
TTCAATGATC TGCTCTGCAG CGTGGATGTG AGTGACCTGT CATTCCACGA TTCCCTGGCC 
ACTTTCATTG CCATACTGAT TGCCCGGCAG TGCTTTTCTC TGGAAGATGT CGTTCAGCAC 
GTAGCACTGC CTTCTCTTCT TGCAGCTGCC TGTGGGGACC CAGATGCTGA GCCTGGGGCC 
AGGATGACTT GTCGGCTTCT CCTGCACCTC TTCAGGACAC CCCAGGTCTG CCTCTTTCCC 
CAGGGAGCAG GAAAATTATT TCCTGGAATT CGTTCTTCTT GTGATCGTCA CCTCTTGGCT 
GCTGCTCACA ACAGCATAGA AGTGGGAGCT GTGTTTGCAG TCTTAAAAGC AATTTTGATG 
CTTGGTGACG CTGAGATCGG CAGCAGCAGT GTCACCCCTG TGAAGAACGA GGAGCTGCCC 
GTGAGGGGCT TGCTGGATGA GATCGCTGAG GATGAAATGT GGGCTTCCTC TCACGCTCTG 
AAATCACGTG GAAAAGCTGT TTCCATAGAA ACCGCCAGTT TGAGCGAGTA TGCTAGATAT 
GTGCTAAGGA CCATTTGCCA GCAGGAATGG GTTGGAGAGC ACTGTTTAAA AGAACCTGAG 
AGGCTGTGCA CAGATAAAGA TCTAATTTTG GATCCCGTTC TTTCAAACAA GCAAGCACAG 
AAACTTCTTC AGCTTATCTG TTATCCTCAT GGTGTGAAAG AATGCTCCGA GGGGGACAAC 
CCTCAGAGGC AGCACATCAA ACGCATACTT CAGAACTTAG ACCAGTGGAC TCTGCGGCAG 
TCTTGGTTGG AGCTGCAACT AATGATCAAG CAGTGCATGA AGGAGCCTGG TTCTGGGTCT 
GTGGCTGAAA TGAACAGCTT GTTGGATAAT ATTGCAAAGG CGACAATAGA GGTGTTTCAG 
CAATCCGCTG ATCTAAACAA TAACTCTTCT AACTCTGGTA TAGGCCTCTT CAATCCAAAC 
TCTGTTGGAA ATGCTGACAC AAGTAATACA AGACAGAATG GTAAAAAGAC ATTCCTAAGT 
TCCTCCGAGT GGCGAGGAGT GTGGCTGGTG GCTCCCTTGA TTGCAAAACT ACCTACCTCA 
GTTCAAGGTA GGGTCTTGAA AGCTGCGGGA GAGGAGCTGG AGAAAGGTCA GCATTTGGGG 
TCATCTTCTA AGAAGGAAAG AGATAGACAG AAGCAAAAGA GCATGTCTCT CTTGAGTCAG 
CAGCCTTTCC TTTCTTTGGT ACTTACTTGC CTTAAGGGAC AGGATGAGCA GCGGGAAGGG 
CTTCTAACAT CACTGCAGAA TCAAGTCAAT CAGATCCTTA GTAACTGGAG GGAAGAACGG 
TACCAGGGTG ATGTTAAAGC TAGGCAAATG ATGCATGAAG CCTTACAGCT TCGTCTGAAT 
TTGGTAGGTG GCATGTTTGA TACTGTGCAG AGGAGTACCC AGTGGACCAC AGACTGGGCA 
CTTCTGCTCC TCCAGATAAT CACTTCGGGA ACAGTTGATA TGCAGACCAA CAACGAATTG 
TTCACAACCG TGCTGGATAT GCTGGGTGTC CTCATCAACG GGACGCTCGC CTCCGACTTG 
TCCAGTGCTT CCCAAGGGGG ACCCGAGGAA AACAAAAGGG CTTACATGAA CTTAGTGAAA 
AAGTTAAAAA AGGAGCTGGG AGACAAGCGG TCAGACAGCA TTGACAAGGT TCGCCAGCTG 
CTCCCTTTGC CAAAGCAGAC ATGTGATATT ATTACCTGTG AGCCAATGGG ATCCTTGATT 
GACACCAAGG GCAACAAAAT TGCGGGGTTT GACTCCATTG ATAAGAAACA GGGTCTTCAG 
GTGTCAACAA AACAAAAGGT GTCCCCTTGG GATTTGTTTG AAGGTCATAA AAATCCTGCT 
CCTCTCTCCT GGGCCTGGTT TGGTACAGTT CGTGTCGACA GGAAGGTGAT CAAATACGAA 
GAGCAGCAGC ATCTGCTCCT GTACCATGCG CACCCCAAAC CCAAGCCGCG GAGCTACTAC 
CTCGAGCCCC TGCCTCTGCC TCCGGAGGAG GAGGAGGAGG AGGAGCCCAC CACCCCTGTC 
TCTCAGGAAC CAGAAAGGAA GTCAGGAGAT CAGGATCAGG GAAAGCATGC CACAGATGAC 
GAGAAGAAGA CAAAGGGCAG GAAGAGGAAG TCAAAGTCAG GCTCTAAGGC TGATGAATAT 
CCACAGAACA ACTTATACAG AGTGCCTCCC AATTACTCTC CCATTCCCTC ACAAATGATG 
CAGCATCCTC AGTCTGCCCT GTGGGGCTAC ACCATCATGG GGCAGCCGCA GCAGCCTGGC 
TTCTTCGTGC AGAACCAGCC CCTCCCTCCA GGTGGTTCCA GGCTGGATCC AACGAGCTCC 
TTCGTCCCAG CCAACACGAA GCAGGCCCTG TCCAACATGC TGCAGCGGCG CTCCGGCACC 
GTGCTGCAGC CCCCTGCCCT CCACGCAGTC ACACCTCAGC AGCAGTTGCT CCAGATGAAA 
CTCCTGCAGC AAGAGCAGCA GCAGCAGCGG CTCCTCAGGC ACCAAGCACA GGCACGGTCT 
CTCCAGCAGG GTCAGCCGAT GGACCCCGCT GCTCTTTTCA CCCCGCAAGT CCGACCCACG 
CCACAGCTAC CGCAGTACCC AGGGCTTCAG CAAGCACAGA GCATGCCCCA CGGGTACACG 
ATGTACAGCA CCCCGATGCC TTTGCAGCAG CAGCAGCAGC CCAGCAGCGT GGTGCTCTCT 
CCTGGCTACA CCCCGCGGGC ATACACAGCC CACTCCAGCC CCGTGCTGAT GGAGCGGCTG 
CGGCCGGGGC AGCCGGGCAG CGTGGCTCAG CAGCAGGCAG CTGCCTACAT GCAGCCCCTG 
GCGGGTGCTC AGCGGTTGAC CCATCAGCCT CTGCAGCCAA ACTCATTGAT AGGGGCAGGC 
CTGGACAGTG CATCAACCGC ACGTACACAT CCAACCTTGA GCTCAGCACA GCTGCCCCAG 
GAGCAAATGC GACAGAGGCA GCAGGAGATC CGACAGCAGA GACTCTTCCA GATGCAGCAG 
GGGCAGCAGC AGCAGACTCT TGATCTTCAG CCTGTGCAGC CACAGCAGCC CCTGGTAAGT 
GGGATGGATG GTGAGCGTGG GGCAGGGGCG TGGGATGAGC GGGTGAAGCT TACAGTGAAT 
TTGCAGTGCT GTTGTCCATG TTTCCTGGAC ATGGCCATTT TTTCCAGATA TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147316.1
CDS	1..6477
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPRQKEDELTAVNVKQGFSNQPAFSGDEHGSARNIVINASKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHNWFTDLAGSKPLTILAKKVPILSKKEDVFAYLAKYSVPLLRAAWLIKMTCAYYAAISEAKIKKRQATDPNIEWTQIITRYLREQLAKVAEFYHVTSSQGNSSVVMPQEMEQALKQWEYNEKLSFYMFQEGMLERHEYLTWILDVLEKIRPTDDDILKLVLPLMLQYSEEFVQSAYLSRRLAYFCARRLSLLLSDGPSLAAAHSPHVIIAPSNPPLAVPSPTAPGPVLSPVQLTCSDFLSCPQHRPLVYGLSCMLQTITLCCPSALVWNYSTNENKNMNPGSPLDLLQVAPSSLPMPGGNSTFNQQVRAKIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRSDSSNSMETLYHKIFWANQNKDNQEAAPSDEAVVMLLCEWAVSCKRSGKHRAMVVAKLLEKRQAEIEAERCGEAEVLDEKESLSSASLTSSSLPVFQNILLRFLDTQAPSLSDPNSDHEKTEFVNLVLLFSEFTRHDVFSHDAYMCTLISRGDLSITATTRPRSPNGETGDEHYSKDHDMKMEDHNIMEHMGTDSGPTSIFDDVDKGDFKADFGSEFPIFSPMPGESCENMNSSLDRRISVASEKSVKRERLREVIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRMILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQEAFPTLETVFTKLQQLSYFDQHQVTSQISSNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRRYHACLILNSEQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYSNVQPSNSNLLWDPEFMLDFIENPSAHSINYSMLGKILSDNAANRYSFVCNALMNVCMGHQDAGRINDIANLCAELTACCTVLSSEWLGVVKALCCSSNHVWGFNDLLCSVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDPDAEPGARMTCRLLLHLFRTPQVCLFPQGAGKLFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAILMLGDAEIGSSSVTPVKNEELPVRGLLDEIAEDEMWASSHALKSRGKAVSIETASLSEYARYVLRTICQQEWVGEHCLKEPERLCTDKDLILDPVLSNKQAQKLLQLICYPHGVKECSEGDNPQRQHIKRILQNLDQWTLRQSWLELQLMIKQCMKEPGSGSVAEMNSLLDNIAKATIEVFQQSADLNNNSSNSGIGLFNPNSVGNADTSNTRQNGKKTFLSSSEWRGVWLVAPLIAKLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQGDVKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMQTNNELFTTVLDMLGVLINGTLASDLSSASQGGPEENKRAYMNLVKKLKKELGDKRSDSIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGHKNPAPLSWAWFGTVRVDRKVIKYEEQQHLLLYHAHPKPKPRSYYLEPLPLPPEEEEEEEPTTPVSQEPERKSGDQDQGKHATDDEKKTKGRKRKSKSGSKADEYPQNNLYRVPPNYSPIPSQMMQHPQSALWGYTIMGQPQQPGFFVQNQPLPPGGSRLDPTSSFVPANTKQALSNMLQRRSGTVLQPPALHAVTPQQQLLQMKLLQQEQQQQRLLRHQAQARSLQQGQPMDPAALFTPQVRPTPQLPQYPGLQQAQSMPHGYTMYSTPMPLQQQQQPSSVVLSPGYTPRAYTAHSSPVLMERLRPGQPGSVAQQQAAAYMQPLAGAQRLTHQPLQPNSLIGAGLDSASTARTHPTLSSAQLPQEQMRQRQQEIRQQRLFQMQQGQQQQTLDLQPVQPQQPLVSGMDGERGAGAWDERVKLTVNLQCCCPCFLDMAIFSRYV

Target ORF information:

RefSeq Version	XM_015291830.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291830.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAACTGCTG 
TTGAAATCCT CCGACCTTGT TGGCAGCTAC ACCACCAGCT TGTGCCTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCT AAGGTGAAGC AAACGATATA CAGCAATGTT 
CAGCCTTCAA ATTCCAATCT GCTGTGGGAC CCGGAGTTCA TGCTTGATTT TATTGAGAAC 
CCTTCTGCTC ACAGCATTAA CTACTCCATG CTGGGCAAGA TCCTGAGTGA CAACGCAGCC 
AACCGCTACA GCTTTGTCTG CAATGCTCTA ATGAATGTGT GCATGGGGCA CCAAGATGCA 
GGGAGGATTA ATGACATAGC AAACCTTTGT GCAGAGCTGA CGGCGTGCTG CACCGTGCTG 
AGCTCGGAGT GGCTGGGGGT TGTCAAAGCT CTTTGCTGCT CTTCCAATCA CGTGTGGGGT 
TTCAATGATC TGCTCTGCAG CGTGGATGTG AGTGACCTGT CATTCCACGA TTCCCTGGCC 
ACTTTCATTG CCATACTGAT TGCCCGGCAG TGCTTTTCTC TGGAAGATGT CGTTCAGCAC 
GTAGCACTGC CTTCTCTTCT TGCAGCTGCC TGTGGGGACC CAGATGCTGA GCCTGGGGCC 
AGGATGACTT GTCGGCTTCT CCTGCACCTC TTCAGGACAC CCCAGGTCTG CCTCTTTCCC 
CAGGGAGCAG GAAAATTATT TCCTGGAATT CGTTCTTCTT GTGATCGTCA CCTCTTGGCT 
GCTGCTCACA ACAGCATAGA AGTGGGAGCT GTGTTTGCAG TCTTAAAAGC AATTTTGATG 
CTTGGTGACG CTGAGATCGG CAGCAGCAGT GTCACCCCTG TGAAGAACGA GGAGCTGCCC 
GTGAGGGGCT TGCTGGATGA GATCGCTGAG GATGAAATGT GGGCTTCCTC TCACGCTCTG 
AAATCACGTG GAAAAGCTGT TTCCATAGAA ACCGCCAGTT TGAGCGAGTA TGCTAGATAT 
GTGCTAAGGA CCATTTGCCA GCAGGAATGG GTTGGAGAGC ACTGTTTAAA AGAACCTGAG 
AGGCTGTGCA CAGATAAAGA TCTAATTTTG GATCCCGTTC TTTCAAACAA GCAAGCACAG 
AAACTTCTTC AGCTTATCTG TTATCCTCAT GGTGTGAAAG AATGCTCCGA GGGGGACAAC 
CCTCAGAGGC AGCACATCAA ACGCATACTT CAGAACTTAG ACCAGTGGAC TCTGCGGCAG 
TCTTGGTTGG AGCTGCAACT AATGATCAAG CAGTGCATGA AGGAGCCTGG TTCTGGGTCT 
GTGGCTGAAA TGAACAGCTT GTTGGATAAT ATTGCAAAGG CGACAATAGA GGTGTTTCAG 
CAATCCGCTG ATCTAAACAA TAACTCTTCT AACTCTGGTA TAGGCCTCTT CAATCCAAAC 
TCTGTTGGAA ATGCTGACAC AAGTAATACA AGACAGAATG GTAAAAAGAC ATTCCTAAGT 
TCCTCCGAGT GGCGAGGAGT GTGGCTGGTG GCTCCCTTGA TTGCAAAACT ACCTACCTCA 
GTTCAAGGTA GGGTCTTGAA AGCTGCGGGA GAGGAGCTGG AGAAAGGTCA GCATTTGGGG 
TCATCTTCTA AGAAGGAAAG AGATAGACAG AAGCAAAAGA GCATGTCTCT CTTGAGTCAG 
CAGCCTTTCC TTTCTTTGGT ACTTACTTGC CTTAAGGGAC AGGATGAGCA GCGGGAAGGG 
CTTCTAACAT CACTGCAGAA TCAAGTCAAT CAGATCCTTA GTAACTGGAG GGAAGAACGG 
TACCAGGGTG ATGTTAAAGC TAGGCAAATG ATGCATGAAG CCTTACAGCT TCGTCTGAAT 
TTGGTAGGTG GCATGTTTGA TACTGTGCAG AGGAGTACCC AGTGGACCAC AGACTGGGCA 
CTTCTGCTCC TCCAGATAAT CACTTCGGGA ACAGTTGATA TGCAGACCAA CAACGAATTG 
TTCACAACCG TGCTGGATAT GCTGGGTGTC CTCATCAACG GGACGCTCGC CTCCGACTTG 
TCCAGTGCTT CCCAAGGGGG ACCCGAGGAA AACAAAAGGG CTTACATGAA CTTAGTGAAA 
AAGTTAAAAA AGGAGCTGGG AGACAAGCGG TCAGACAGCA TTGACAAGGT TCGCCAGCTG 
CTCCCTTTGC CAAAGCAGAC ATGTGATATT ATTACCTGTG AGCCAATGGG ATCCTTGATT 
GACACCAAGG GCAACAAAAT TGCGGGGTTT GACTCCATTG ATAAGAAACA GGGTCTTCAG 
GTGTCAACAA AACAAAAGGT GTCCCCTTGG GATTTGTTTG AAGGTCATAA AAATCCTGCT 
CCTCTCTCCT GGGCCTGGTT TGGTACAGTT CGTGTCGACA GGAAGGTGAT CAAATACGAA 
GAGCAGCAGC ATCTGCTCCT GTACCATGCG CACCCCAAAC CCAAGCCGCG GAGCTACTAC 
CTCGAGCCCC TGCCTCTGCC TCCGGAGGAG GAGGAGGAGG AGGAGCCCAC CACCCCTGTC 
TCTCAGGAAC CAGAAAGGAA GTCAGGAGAT CAGGATCAGG GAAAGCATGC CACAGATGAC 
GAGAAGAAGA CAAAGGGCAG GAAGAGGAAG TCAAAGTCAG GCTCTAAGGC TGATGAATAT 
CCACAGAACA ACTTATACAG AGTGCCTCCC AATTACTCTC CCATTCCCTC ACAAATGATG 
CAGCATCCTC AGTCTGCCCT GTGGGGCTAC ACCATCATGG GGCAGCCGCA GCAGCCTGGC 
TTCTTCGTGC AGAACCAGCC CCTCCCTCCA GGTGGTTCCA GGCTGGATCC AACGAGCTCC 
TTCGTCCCAG CCAACACGAA GCAGGCCCTG TCCAACATGC TGCAGCGGCG CTCCGGCACC 
GTGCTGCAGC CCCCTGCCCT CCACGCAGTC ACACCTCAGC AGCAGTTGCT CCAGATGAAA 
CTCCTGCAGC AAGAGCAGCA GCAGCAGCGG CTCCTCAGGC ACCAAGCACA GGCACGGTCT 
CTCCAGCAGG GTCAGCCGAT GGACCCCGCT GCTCTTTTCA CCCCGCAAGT CCGACCCACG 
CCACAGCTAC CGCAGTACCC AGGGCTTCAG CAAGCACAGA GCATGCCCCA CGGGTACACG 
ATGTACAGCA CCCCGATGCC TTTGCAGCAG CAGCAGCAGC CCAGCAGCGT GGTGCTCTCT 
CCTGGCTACA CCCCGCGGGC ATACACAGCC CACTCCAGCC CCGTGCTGAT GGAGCGGCTG 
CGGCCGGGGC AGCCGGGCAG CGTGGCTCAG CAGCAGGCAG CTGCCTACAT GCAGCCCCTG 
GCGGGTGCTC AGCGGTTGAC CCATCAGCCT CTGCAGCCAA ACTCATTGAT AGGGGCAGGC 
CTGGACAGTG CATCAACCGC ACGTACACAT CCAACCTTGA GCTCAGCACA GCTGCCCCAG 
GAGCAAATGC GACAGAGGCA GCAGGAGATC CGACAGCAGA GACTCTTCCA GATGCAGCAG 
GGGCAGCAGC AGCAGACTCT TGATCTTCAG CCTGTGCAGC CACAGCAGCC CCTGGTAAGT 
GGGATGGATG GTGAGCGTGG GGCAGGGGCG TGGGATGAGC GGGTGAAGCT TACAGTGAAT 
TTGCAGTGCT GTTGTCCATG TTTCCTGGAC ATGGCCATTT TTTCCAGATA TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_015291831.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147317.1 Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X4, mRNA.

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CloneID	OGa40170
Clone ID Related Accession (Same CDS sequence)	XM_015291831.1
Accession Version	XM_015291831.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAACTGCTG 
TTGAAATCCT CCGACCTTGT TGGCAGCTAC ACCACCAGCT TGTGCCTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCT AAGGTGAAGC AAACGATATA CAGCAATGTT 
CAGCCTTCAA ATTCCAATCT GCTGTGGGAC CCGGAGTTCA TGCTTGATTT TATTGAGAAC 
CCTTCTGCTC ACAGCATTAA CTACTCCATG CTGGGCAAGA TCCTGAGTGA CAACGCAGCC 
AACCGCTACA GCTTTGTCTG CAATGCTCTA ATGAATGTGT GCATGGGGCA CCAAGATGCA 
GGGAGGATTA ATGACATAGC AAACCTTTGT GCAGAGCTGA CGGCGTGCTG CACCGTGCTG 
AGCTCGGAGT GGCTGGGGGT TGTCAAAGCT CTTTGCTGCT CTTCCAATCA CGTGTGGGGT 
TTCAATGATC TGCTCTGCAG CGTGGATGTG AGTGACCTGT CATTCCACGA TTCCCTGGCC 
ACTTTCATTG CCATACTGAT TGCCCGGCAG TGCTTTTCTC TGGAAGATGT CGTTCAGCAC 
GTAGCACTGC CTTCTCTTCT TGCAGCTGCC TGTGGGGACC CAGATGCTGA GCCTGGGGCC 
AGGATGACTT GTCGGCTTCT CCTGCACCTC TTCAGGACAC CCCAGGTCTG CCTCTTTCCC 
CAGGGAGCAG GAAAATTATT TCCTGGAATT CGTTCTTCTT GTGATCGTCA CCTCTTGGCT 
GCTGCTCACA ACAGCATAGA AGTGGGAGCT GTGTTTGCAG TCTTAAAAGC AATTTTGATG 
CTTGGTGACG CTGAGATCGG CAGCAGCAGT GTCACCCCTG TGAAGAACGA GGAGCTGCCC 
GTGAGGGGCT TGCTGGATGA GATCGCTGAG GATGAAATGT GGGCTTCCTC TCACGCTCTG 
AAATCACGTG GAAAAGCTGT TTCCATAGAA ACCGCCAGTT TGAGCGAGTA TGCTAGATAT 
GTGCTAAGGA CCATTTGCCA GCAGGAATGG GTTGGAGAGC ACTGTTTAAA AGAACCTGAG 
AGGCTGTGCA CAGATAAAGA TCTAATTTTG GATCCCGTTC TTTCAAACAA GCAAGCACAG 
AAACTTCTTC AGCTTATCTG TTATCCTCAT GGTGTGAAAG AATGCTCCGA GGGGGACAAC 
CCTCAGAGGC AGCACATCAA ACGCATACTT CAGAACTTAG ACCAGTGGAC TCTGCGGCAG 
TCTTGGTTGG AGCTGCAACT AATGATCAAG CAGTGCATGA AGGAGCCTGG TTCTGGGTCT 
GTGGCTGAAA TGAACAGCTT GTTGGATAAT ATTGCAAAGG CGACAATAGA GGTGTTTCAG 
CAATCCGCTG ATCTAAACAA TAACTCTTCT AACTCTGGTA TAGGCCTCTT CAATCCAAAC 
TCTGTTGGAA ATGCTGACAC AAGTAATACA AGACAGAATG GTAAAAAGAC ATTCCTAAGT 
TCCTCCGAGT GGCGAGGAGT GTGGCTGGTG GCTCCCTTGA TTGCAAAACT ACCTACCTCA 
GTTCAAGGTA GGGTCTTGAA AGCTGCGGGA GAGGAGCTGG AGAAAGGTCA GCATTTGGGG 
TCATCTTCTA AGAAGGAAAG AGATAGACAG AAGCAAAAGA GCATGTCTCT CTTGAGTCAG 
CAGCCTTTCC TTTCTTTGGT ACTTACTTGC CTTAAGGGAC AGGATGAGCA GCGGGAAGGG 
CTTCTAACAT CACTGCAGAA TCAAGTCAAT CAGATCCTTA GTAACTGGAG GGAAGAACGG 
TACCAGGGTG ATGTTAAAGC TAGGCAAATG ATGCATGAAG CCTTACAGCT TCGTCTGAAT 
TTGGTAGGTG GCATGTTTGA TACTGTGCAG AGGAGTACCC AGTGGACCAC AGACTGGGCA 
CTTCTGCTCC TCCAGATAAT CACTTCGGGA ACAGTTGATA TGCAGACCAA CAACGAATTG 
TTCACAACCG TGCTGGATAT GCTGGGTGTC CTCATCAACG GGACGCTCGC CTCCGACTTG 
TCCAGTGCTT CCCAAGGGGG ACCCGAGGAA AACAAAAGGG CTTACATGAA CTTAGTGAAA 
AAGTTAAAAA AGGAGCTGGG AGACAAGCGG TCAGACAGCA TTGACAAGGT TCGCCAGCTG 
CTCCCTTTGC CAAAGCAGAC ATGTGATATT ATTACCTGTG AGCCAATGGG ATCCTTGATT 
GACACCAAGG GCAACAAAAT TGCGGGGTTT GACTCCATTG ATAAGAAACA GGGTCTTCAG 
GTGTCAACAA AACAAAAGGT GTCCCCTTGG GATTTGTTTG AAGGTCATAA AAATCCTGCT 
CCTCTCTCCT GGGCCTGGTT TGGTACAGTT CGTGTCGACA GGAAGGTGAT CAAATACGAA 
GAGCAGCAGC ATCTGCTCCT GTACCATGCG CACCCCAAAC CCAAGCCGCG GAGCTACTAC 
CTCGAGCCCC TGCCTCTGCC TCCGGAGGAG GAGGAGGAGG AGGAGCCCAC CACCCCTGTC 
TCTCAGGAAC CAGAAAGGAA GTCAGGAGAT CAGGATCAGG GAAAGCATGC CACAGATGAC 
GAGAAGAAGA CAAAGGGCAG GAAGAGGAAG TCAAAGTCAG GCTCTAAGGC TGATGAATAT 
CCACAGAACA ACTTATACAG AGTGCCTCCC AATTACTCTC CCATTCCCTC ACAAATGATG 
CAGCATCCTC AGTCTGCCCT GTGGGGCTAC ACCATCATGG GGCAGCCGCA GCAGCCTGGC 
TTCTTCGTGC AGAACCAGCC CCTCCCTCCA GGTGGTTCCA GGCTGGATCC AACGAGCTCC 
TTCGTCCCAG CCAACACGAA GCAGGCCCTG TCCAACATGC TGCAGCGGCG CTCCGGCACC 
GTGCTGCAGC CCCCTGCCCT CCACGCAGTC ACACCTCAGC AGCAGTTGCT CCAGATGAAA 
CTCCTGCAGC AAGAGCAGCA GCAGCAGCGG CTCCTCAGGC ACCAAGCACA GGGTCAGCCG 
ATGGACCCCG CTGCTCTTTT CACCCCGCAA GTCCGACCCA CGCCACAGCT ACCGCAGTAC 
CCAGGGCTTC AGCAAGCACA GAGCATGCCC CACGGGTACA CGATGTACAG CACCCCGATG 
CCTTTGCAGC AGCAGCAGCA GCCCAGCAGC GTGGTGCTCT CTCCTGGCTA CACCCCGCGG 
GCATACACAG CCCACTCCAG CCCCGTGCTG ATGGAGCGGC TGCGGCCGGG GCAGCCGGGC 
AGCGTGGCTC AGCAGCAGGC AGCTGCCTAC ATGCAGCCCC TGGCGGGTGC TCAGCGGTTG 
ACCCATCAGC CTCTGCAGCC AAACTCATTG ATAGGGGCAG GCCTGGACAG TGCATCAACC 
GCACGTACAC ATCCAACCTT GAGCTCAGCA CAGCTGCCCC AGGAGCAAAT GCGACAGAGG 
CAGCAGGAGA TCCGACAGCA GAGACTCTTC CAGATGCAGC AGGGGCAGCA GCAGCAGACT 
CTTGATCTTC AGCCTGTGCA GCCACAGCAG CCCCTGGTAA GTGGGATGGA TGGTGAGCGT 
GGGGCAGGGG CGTGGGATGA GCGGGTGAAG CTTACAGTGA ATTTGCAGTG CTGTTGTCCA 
TGTTTCCTGG ACATGGCCAT TTTTTCCAGA TATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147317.1
CDS	142..6600
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPRQKEDELTAVNVKQGFSNQPAFSGDEHGSARNIVINASKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHNWFTDLAGSKPLTILAKKVPILSKKEDVFAYLAKYSVPLLRAAWLIKMTCAYYAAISEAKIKKRQATDPNIEWTQIITRYLREQLAKVAEFYHVTSSQGNSSVVMPQEMEQALKQWEYNEKLSFYMFQEGMLERHEYLTWILDVLEKIRPTDDDILKLVLPLMLQYSEEFVQSAYLSRRLAYFCARRLSLLLSDGPSLAAAHSPHVIIAPSNPPLAVPSPTAPGPVLSPVQLTCSDFLSCPQHRPLVYGLSCMLQTITLCCPSALVWNYSTNENKNMNPGSPLDLLQVAPSSLPMPGGNSTFNQQVRAKIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRSDSSNSMETLYHKIFWANQNKDNQEAAPSDEAVVMLLCEWAVSCKRSGKHRAMVVAKLLEKRQAEIEAERCGEAEVLDEKESLSSASLTSSSLPVFQNILLRFLDTQAPSLSDPNSDHEKTEFVNLVLLFSEFTRHDVFSHDAYMCTLISRGDLSITATTRPRSPNGETGDEHYSKDHDMKMEDHNIMEHMGTDSGPTSIFDDVDKGDFKADFGSEFPIFSPMPGESCENMNSSLDRRISVASEKSVKRERLREVIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRMILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQEAFPTLETVFTKLQQLSYFDQHQVTSQISSNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRRYHACLILNSEQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYSNVQPSNSNLLWDPEFMLDFIENPSAHSINYSMLGKILSDNAANRYSFVCNALMNVCMGHQDAGRINDIANLCAELTACCTVLSSEWLGVVKALCCSSNHVWGFNDLLCSVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDPDAEPGARMTCRLLLHLFRTPQVCLFPQGAGKLFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAILMLGDAEIGSSSVTPVKNEELPVRGLLDEIAEDEMWASSHALKSRGKAVSIETASLSEYARYVLRTICQQEWVGEHCLKEPERLCTDKDLILDPVLSNKQAQKLLQLICYPHGVKECSEGDNPQRQHIKRILQNLDQWTLRQSWLELQLMIKQCMKEPGSGSVAEMNSLLDNIAKATIEVFQQSADLNNNSSNSGIGLFNPNSVGNADTSNTRQNGKKTFLSSSEWRGVWLVAPLIAKLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQGDVKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMQTNNELFTTVLDMLGVLINGTLASDLSSASQGGPEENKRAYMNLVKKLKKELGDKRSDSIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGHKNPAPLSWAWFGTVRVDRKVIKYEEQQHLLLYHAHPKPKPRSYYLEPLPLPPEEEEEEEPTTPVSQEPERKSGDQDQGKHATDDEKKTKGRKRKSKSGSKADEYPQNNLYRVPPNYSPIPSQMMQHPQSALWGYTIMGQPQQPGFFVQNQPLPPGGSRLDPTSSFVPANTKQALSNMLQRRSGTVLQPPALHAVTPQQQLLQMKLLQQEQQQQRLLRHQAQGQPMDPAALFTPQVRPTPQLPQYPGLQQAQSMPHGYTMYSTPMPLQQQQQPSSVVLSPGYTPRAYTAHSSPVLMERLRPGQPGSVAQQQAAAYMQPLAGAQRLTHQPLQPNSLIGAGLDSASTARTHPTLSSAQLPQEQMRQRQQEIRQQRLFQMQQGQQQQTLDLQPVQPQQPLVSGMDGERGAGAWDERVKLTVNLQCCCPCFLDMAIFSRYV

Target ORF information:

RefSeq Version	XM_015291831.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291831.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAACTGCTG 
TTGAAATCCT CCGACCTTGT TGGCAGCTAC ACCACCAGCT TGTGCCTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCT AAGGTGAAGC AAACGATATA CAGCAATGTT 
CAGCCTTCAA ATTCCAATCT GCTGTGGGAC CCGGAGTTCA TGCTTGATTT TATTGAGAAC 
CCTTCTGCTC ACAGCATTAA CTACTCCATG CTGGGCAAGA TCCTGAGTGA CAACGCAGCC 
AACCGCTACA GCTTTGTCTG CAATGCTCTA ATGAATGTGT GCATGGGGCA CCAAGATGCA 
GGGAGGATTA ATGACATAGC AAACCTTTGT GCAGAGCTGA CGGCGTGCTG CACCGTGCTG 
AGCTCGGAGT GGCTGGGGGT TGTCAAAGCT CTTTGCTGCT CTTCCAATCA CGTGTGGGGT 
TTCAATGATC TGCTCTGCAG CGTGGATGTG AGTGACCTGT CATTCCACGA TTCCCTGGCC 
ACTTTCATTG CCATACTGAT TGCCCGGCAG TGCTTTTCTC TGGAAGATGT CGTTCAGCAC 
GTAGCACTGC CTTCTCTTCT TGCAGCTGCC TGTGGGGACC CAGATGCTGA GCCTGGGGCC 
AGGATGACTT GTCGGCTTCT CCTGCACCTC TTCAGGACAC CCCAGGTCTG CCTCTTTCCC 
CAGGGAGCAG GAAAATTATT TCCTGGAATT CGTTCTTCTT GTGATCGTCA CCTCTTGGCT 
GCTGCTCACA ACAGCATAGA AGTGGGAGCT GTGTTTGCAG TCTTAAAAGC AATTTTGATG 
CTTGGTGACG CTGAGATCGG CAGCAGCAGT GTCACCCCTG TGAAGAACGA GGAGCTGCCC 
GTGAGGGGCT TGCTGGATGA GATCGCTGAG GATGAAATGT GGGCTTCCTC TCACGCTCTG 
AAATCACGTG GAAAAGCTGT TTCCATAGAA ACCGCCAGTT TGAGCGAGTA TGCTAGATAT 
GTGCTAAGGA CCATTTGCCA GCAGGAATGG GTTGGAGAGC ACTGTTTAAA AGAACCTGAG 
AGGCTGTGCA CAGATAAAGA TCTAATTTTG GATCCCGTTC TTTCAAACAA GCAAGCACAG 
AAACTTCTTC AGCTTATCTG TTATCCTCAT GGTGTGAAAG AATGCTCCGA GGGGGACAAC 
CCTCAGAGGC AGCACATCAA ACGCATACTT CAGAACTTAG ACCAGTGGAC TCTGCGGCAG 
TCTTGGTTGG AGCTGCAACT AATGATCAAG CAGTGCATGA AGGAGCCTGG TTCTGGGTCT 
GTGGCTGAAA TGAACAGCTT GTTGGATAAT ATTGCAAAGG CGACAATAGA GGTGTTTCAG 
CAATCCGCTG ATCTAAACAA TAACTCTTCT AACTCTGGTA TAGGCCTCTT CAATCCAAAC 
TCTGTTGGAA ATGCTGACAC AAGTAATACA AGACAGAATG GTAAAAAGAC ATTCCTAAGT 
TCCTCCGAGT GGCGAGGAGT GTGGCTGGTG GCTCCCTTGA TTGCAAAACT ACCTACCTCA 
GTTCAAGGTA GGGTCTTGAA AGCTGCGGGA GAGGAGCTGG AGAAAGGTCA GCATTTGGGG 
TCATCTTCTA AGAAGGAAAG AGATAGACAG AAGCAAAAGA GCATGTCTCT CTTGAGTCAG 
CAGCCTTTCC TTTCTTTGGT ACTTACTTGC CTTAAGGGAC AGGATGAGCA GCGGGAAGGG 
CTTCTAACAT CACTGCAGAA TCAAGTCAAT CAGATCCTTA GTAACTGGAG GGAAGAACGG 
TACCAGGGTG ATGTTAAAGC TAGGCAAATG ATGCATGAAG CCTTACAGCT TCGTCTGAAT 
TTGGTAGGTG GCATGTTTGA TACTGTGCAG AGGAGTACCC AGTGGACCAC AGACTGGGCA 
CTTCTGCTCC TCCAGATAAT CACTTCGGGA ACAGTTGATA TGCAGACCAA CAACGAATTG 
TTCACAACCG TGCTGGATAT GCTGGGTGTC CTCATCAACG GGACGCTCGC CTCCGACTTG 
TCCAGTGCTT CCCAAGGGGG ACCCGAGGAA AACAAAAGGG CTTACATGAA CTTAGTGAAA 
AAGTTAAAAA AGGAGCTGGG AGACAAGCGG TCAGACAGCA TTGACAAGGT TCGCCAGCTG 
CTCCCTTTGC CAAAGCAGAC ATGTGATATT ATTACCTGTG AGCCAATGGG ATCCTTGATT 
GACACCAAGG GCAACAAAAT TGCGGGGTTT GACTCCATTG ATAAGAAACA GGGTCTTCAG 
GTGTCAACAA AACAAAAGGT GTCCCCTTGG GATTTGTTTG AAGGTCATAA AAATCCTGCT 
CCTCTCTCCT GGGCCTGGTT TGGTACAGTT CGTGTCGACA GGAAGGTGAT CAAATACGAA 
GAGCAGCAGC ATCTGCTCCT GTACCATGCG CACCCCAAAC CCAAGCCGCG GAGCTACTAC 
CTCGAGCCCC TGCCTCTGCC TCCGGAGGAG GAGGAGGAGG AGGAGCCCAC CACCCCTGTC 
TCTCAGGAAC CAGAAAGGAA GTCAGGAGAT CAGGATCAGG GAAAGCATGC CACAGATGAC 
GAGAAGAAGA CAAAGGGCAG GAAGAGGAAG TCAAAGTCAG GCTCTAAGGC TGATGAATAT 
CCACAGAACA ACTTATACAG AGTGCCTCCC AATTACTCTC CCATTCCCTC ACAAATGATG 
CAGCATCCTC AGTCTGCCCT GTGGGGCTAC ACCATCATGG GGCAGCCGCA GCAGCCTGGC 
TTCTTCGTGC AGAACCAGCC CCTCCCTCCA GGTGGTTCCA GGCTGGATCC AACGAGCTCC 
TTCGTCCCAG CCAACACGAA GCAGGCCCTG TCCAACATGC TGCAGCGGCG CTCCGGCACC 
GTGCTGCAGC CCCCTGCCCT CCACGCAGTC ACACCTCAGC AGCAGTTGCT CCAGATGAAA 
CTCCTGCAGC AAGAGCAGCA GCAGCAGCGG CTCCTCAGGC ACCAAGCACA GGGTCAGCCG 
ATGGACCCCG CTGCTCTTTT CACCCCGCAA GTCCGACCCA CGCCACAGCT ACCGCAGTAC 
CCAGGGCTTC AGCAAGCACA GAGCATGCCC CACGGGTACA CGATGTACAG CACCCCGATG 
CCTTTGCAGC AGCAGCAGCA GCCCAGCAGC GTGGTGCTCT CTCCTGGCTA CACCCCGCGG 
GCATACACAG CCCACTCCAG CCCCGTGCTG ATGGAGCGGC TGCGGCCGGG GCAGCCGGGC 
AGCGTGGCTC AGCAGCAGGC AGCTGCCTAC ATGCAGCCCC TGGCGGGTGC TCAGCGGTTG 
ACCCATCAGC CTCTGCAGCC AAACTCATTG ATAGGGGCAG GCCTGGACAG TGCATCAACC 
GCACGTACAC ATCCAACCTT GAGCTCAGCA CAGCTGCCCC AGGAGCAAAT GCGACAGAGG 
CAGCAGGAGA TCCGACAGCA GAGACTCTTC CAGATGCAGC AGGGGCAGCA GCAGCAGACT 
CTTGATCTTC AGCCTGTGCA GCCACAGCAG CCCCTGGTAA GTGGGATGGA TGGTGAGCGT 
GGGGCAGGGG CGTGGGATGA GCGGGTGAAG CTTACAGTGA ATTTGCAGTG CTGTTGTCCA 
TGTTTCCTGG ACATGGCCAT TTTTTCCAGA TATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_015291832.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147318.1 Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X5, mRNA.

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CloneID	OGa40171
Clone ID Related Accession (Same CDS sequence)	XM_015291832.1
Accession Version	XM_015291832.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAACTGCTG 
TTGAAATCCT CCGACCTTGT TGGCAGCTAC ACCACCAGCT TGTGCCTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCT AAGGTGAAGC AAACGATATA CAGCAATGTT 
CAGCCTTCAA ATTCCAATCT GCTGTGGGAC CCGGAGTTCA TGCTTGATTT TATTGAGAAC 
CCTTCTGCTC ACAGCATTAA CTACTCCATG CTGGGCAAGA TCCTGAGTGA CAACGCAGCC 
AACCGCTACA GCTTTGTCTG CAATGCTCTA ATGAATGTGT GCATGGGGCA CCAAGATGCA 
GGGAGGATTA ATGACATAGC AAACCTTTGT GCAGAGCTGA CGGCGTGCTG CACCGTGCTG 
AGCTCGGAGT GGCTGGGGGT TGTCAAAGCT CTTTGCTGCT CTTCCAATCA CGTGTGGGGT 
TTCAATGATC TGCTCTGCAG CGTGGATGTG AGTGACCTGT CATTCCACGA TTCCCTGGCC 
ACTTTCATTG CCATACTGAT TGCCCGGCAG TGCTTTTCTC TGGAAGATGT CGTTCAGCAC 
GTAGCACTGC CTTCTCTTCT TGCAGCTGGA AAATTATTTC CTGGAATTCG TTCTTCTTGT 
GATCGTCACC TCTTGGCTGC TGCTCACAAC AGCATAGAAG TGGGAGCTGT GTTTGCAGTC 
TTAAAAGCAA TTTTGATGCT TGGTGACGCT GAGATCGGCA GCAGCAGTGT CACCCCTGTG 
AAGAACGAGG AGCTGCCCGT GAGGGGCTTG CTGGATGAGA TCGCTGAGGA TGAAATGTGG 
GCTTCCTCTC ACGCTCTGAA ATCACGTGGA AAAGCTGTTT CCATAGAAAC CGCCAGTTTG 
AGCGAGTATG CTAGATATGT GCTAAGGACC ATTTGCCAGC AGGAATGGGT TGGAGAGCAC 
TGTTTAAAAG AACCTGAGAG GCTGTGCACA GATAAAGATC TAATTTTGGA TCCCGTTCTT 
TCAAACAAGC AAGCACAGAA ACTTCTTCAG CTTATCTGTT ATCCTCATGG TGTGAAAGAA 
TGCTCCGAGG GGGACAACCC TCAGAGGCAG CACATCAAAC GCATACTTCA GAACTTAGAC 
CAGTGGACTC TGCGGCAGTC TTGGTTGGAG CTGCAACTAA TGATCAAGCA GTGCATGAAG 
GAGCCTGGTT CTGGGTCTGT GGCTGAAATG AACAGCTTGT TGGATAATAT TGCAAAGGCG 
ACAATAGAGG TGTTTCAGCA ATCCGCTGAT CTAAACAATA ACTCTTCTAA CTCTGGTATA 
GGCCTCTTCA ATCCAAACTC TGTTGGAAAT GCTGACACAA GTAATACAAG ACAGAATGGT 
AAAAAGACAT TCCTAAGTTC CTCCGAGTGG CGAGGAGTGT GGCTGGTGGC TCCCTTGATT 
GCAAAACTAC CTACCTCAGT TCAAGGTAGG GTCTTGAAAG CTGCGGGAGA GGAGCTGGAG 
AAAGGTCAGC ATTTGGGGTC ATCTTCTAAG AAGGAAAGAG ATAGACAGAA GCAAAAGAGC 
ATGTCTCTCT TGAGTCAGCA GCCTTTCCTT TCTTTGGTAC TTACTTGCCT TAAGGGACAG 
GATGAGCAGC GGGAAGGGCT TCTAACATCA CTGCAGAATC AAGTCAATCA GATCCTTAGT 
AACTGGAGGG AAGAACGGTA CCAGGGTGAT GTTAAAGCTA GGCAAATGAT GCATGAAGCC 
TTACAGCTTC GTCTGAATTT GGTAGGTGGC ATGTTTGATA CTGTGCAGAG GAGTACCCAG 
TGGACCACAG ACTGGGCACT TCTGCTCCTC CAGATAATCA CTTCGGGAAC AGTTGATATG 
CAGACCAACA ACGAATTGTT CACAACCGTG CTGGATATGC TGGGTGTCCT CATCAACGGG 
ACGCTCGCCT CCGACTTGTC CAGTGCTTCC CAAGGGGGAC CCGAGGAAAA CAAAAGGGCT 
TACATGAACT TAGTGAAAAA GTTAAAAAAG GAGCTGGGAG ACAAGCGGTC AGACAGCATT 
GACAAGGTTC GCCAGCTGCT CCCTTTGCCA AAGCAGACAT GTGATATTAT TACCTGTGAG 
CCAATGGGAT CCTTGATTGA CACCAAGGGC AACAAAATTG CGGGGTTTGA CTCCATTGAT 
AAGAAACAGG GTCTTCAGGT GTCAACAAAA CAAAAGGTGT CCCCTTGGGA TTTGTTTGAA 
GGTCATAAAA ATCCTGCTCC TCTCTCCTGG GCCTGGTTTG GTACAGTTCG TGTCGACAGG 
AAGGTGATCA AATACGAAGA GCAGCAGCAT CTGCTCCTGT ACCATGCGCA CCCCAAACCC 
AAGCCGCGGA GCTACTACCT CGAGCCCCTG CCTCTGCCTC CGGAGGAGGA GGAGGAGGAG 
GAGCCCACCA CCCCTGTCTC TCAGGAACCA GAAAGGAAGT CAGGAGATCA GGATCAGGGA 
AAGCATGCCA CAGATGACGA GAAGAAGACA AAGGGCAGGA AGAGGAAGTC AAAGTCAGGC 
TCTAAGGCTG ATGAATATCC ACAGAACAAC TTATACAGAG TGCCTCCCAA TTACTCTCCC 
ATTCCCTCAC AAATGATGCA GCATCCTCAG TCTGCCCTGT GGGGCTACAC CATCATGGGG 
CAGCCGCAGC AGCCTGGCTT CTTCGTGCAG AACCAGCCCC TCCCTCCAGG TGGTTCCAGG 
CTGGATCCAA CGAGCTCCTT CGTCCCAGCC AACACGAAGC AGGCCCTGTC CAACATGCTG 
CAGCGGCGCT CCGGCACCGT GCTGCAGCCC CCTGCCCTCC ACGCAGTCAC ACCTCAGCAG 
CAGTTGCTCC AGATGAAACT CCTGCAGCAA GAGCAGCAGC AGCAGCGGCT CCTCAGGCAC 
CAAGCACAGG CACGGTCTCT CCAGCAGGAT GTCAGTAGCT CTGCCGTATC CCTTCAGGAT 
GGCTTTGACA ACATAATGGG TCAGCCGATG GACCCCGCTG CTCTTTTCAC CCCGCAAGTC 
CGACCCACGC CACAGCTACC GCAGTACCCA GGGCTTCAGC AAGCACAGAG CATGCCCCAC 
GGGTACACGA TGTACAGCAC CCCGATGCCT TTGCAGCAGC AGCAGCAGCC CAGCAGCGTG 
GTGCTCTCTC CTGGCTACAC CCCGCGGGCA TACACAGCCC ACTCCAGCCC CGTGCTGATG 
GAGCGGCTGC GGCCGGGGCA GCCGGGCAGC GTGGCTCAGC AGCAGGCAGC TGCCTACATG 
CAGCCCCTGG CGGGTGCTCA GCGGTTGACC CATCAGCCTC TGCAGCCAAA CTCATTGATA 
GGGGCAGGCC TGGACAGTGC ATCAACCGCA CGTACACATC CAACCTTGAG CTCAGCACAG 
CTGCCCCAGG AGCAAATGCG ACAGAGGCAG CAGGAGATCC GACAGCAGAG ACTCTTCCAG 
ATGCAGCAGG GGCAGCAGCA GCAGACTCTT GATCTTCAGC CTGTGCAGCC ACAGCAGCCC 
CTGGTAAGTG GGATGGATGG TGAGCGTGGG GCAGGGGCGT GGGATGAGCG GGTGAAGCTT 
ACAGTGAATT TGCAGTGCTG TTGTCCATGT TTCCTGGACA TGGCCATTTT TTCCAGATAT 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147318.1
CDS	142..6567
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPRQKEDELTAVNVKQGFSNQPAFSGDEHGSARNIVINASKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHNWFTDLAGSKPLTILAKKVPILSKKEDVFAYLAKYSVPLLRAAWLIKMTCAYYAAISEAKIKKRQATDPNIEWTQIITRYLREQLAKVAEFYHVTSSQGNSSVVMPQEMEQALKQWEYNEKLSFYMFQEGMLERHEYLTWILDVLEKIRPTDDDILKLVLPLMLQYSEEFVQSAYLSRRLAYFCARRLSLLLSDGPSLAAAHSPHVIIAPSNPPLAVPSPTAPGPVLSPVQLTCSDFLSCPQHRPLVYGLSCMLQTITLCCPSALVWNYSTNENKNMNPGSPLDLLQVAPSSLPMPGGNSTFNQQVRAKIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRSDSSNSMETLYHKIFWANQNKDNQEAAPSDEAVVMLLCEWAVSCKRSGKHRAMVVAKLLEKRQAEIEAERCGEAEVLDEKESLSSASLTSSSLPVFQNILLRFLDTQAPSLSDPNSDHEKTEFVNLVLLFSEFTRHDVFSHDAYMCTLISRGDLSITATTRPRSPNGETGDEHYSKDHDMKMEDHNIMEHMGTDSGPTSIFDDVDKGDFKADFGSEFPIFSPMPGESCENMNSSLDRRISVASEKSVKRERLREVIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRMILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQEAFPTLETVFTKLQQLSYFDQHQVTSQISSNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRRYHACLILNSEQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYSNVQPSNSNLLWDPEFMLDFIENPSAHSINYSMLGKILSDNAANRYSFVCNALMNVCMGHQDAGRINDIANLCAELTACCTVLSSEWLGVVKALCCSSNHVWGFNDLLCSVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAGKLFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAILMLGDAEIGSSSVTPVKNEELPVRGLLDEIAEDEMWASSHALKSRGKAVSIETASLSEYARYVLRTICQQEWVGEHCLKEPERLCTDKDLILDPVLSNKQAQKLLQLICYPHGVKECSEGDNPQRQHIKRILQNLDQWTLRQSWLELQLMIKQCMKEPGSGSVAEMNSLLDNIAKATIEVFQQSADLNNNSSNSGIGLFNPNSVGNADTSNTRQNGKKTFLSSSEWRGVWLVAPLIAKLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQGDVKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMQTNNELFTTVLDMLGVLINGTLASDLSSASQGGPEENKRAYMNLVKKLKKELGDKRSDSIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGHKNPAPLSWAWFGTVRVDRKVIKYEEQQHLLLYHAHPKPKPRSYYLEPLPLPPEEEEEEEPTTPVSQEPERKSGDQDQGKHATDDEKKTKGRKRKSKSGSKADEYPQNNLYRVPPNYSPIPSQMMQHPQSALWGYTIMGQPQQPGFFVQNQPLPPGGSRLDPTSSFVPANTKQALSNMLQRRSGTVLQPPALHAVTPQQQLLQMKLLQQEQQQQRLLRHQAQARSLQQDVSSSAVSLQDGFDNIMGQPMDPAALFTPQVRPTPQLPQYPGLQQAQSMPHGYTMYSTPMPLQQQQQPSSVVLSPGYTPRAYTAHSSPVLMERLRPGQPGSVAQQQAAAYMQPLAGAQRLTHQPLQPNSLIGAGLDSASTARTHPTLSSAQLPQEQMRQRQQEIRQQRLFQMQQGQQQQTLDLQPVQPQQPLVSGMDGERGAGAWDERVKLTVNLQCCCPCFLDMAIFSRYV

Target ORF information:

RefSeq Version	XM_015291832.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291832.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAACTGCTG 
TTGAAATCCT CCGACCTTGT TGGCAGCTAC ACCACCAGCT TGTGCCTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCT AAGGTGAAGC AAACGATATA CAGCAATGTT 
CAGCCTTCAA ATTCCAATCT GCTGTGGGAC CCGGAGTTCA TGCTTGATTT TATTGAGAAC 
CCTTCTGCTC ACAGCATTAA CTACTCCATG CTGGGCAAGA TCCTGAGTGA CAACGCAGCC 
AACCGCTACA GCTTTGTCTG CAATGCTCTA ATGAATGTGT GCATGGGGCA CCAAGATGCA 
GGGAGGATTA ATGACATAGC AAACCTTTGT GCAGAGCTGA CGGCGTGCTG CACCGTGCTG 
AGCTCGGAGT GGCTGGGGGT TGTCAAAGCT CTTTGCTGCT CTTCCAATCA CGTGTGGGGT 
TTCAATGATC TGCTCTGCAG CGTGGATGTG AGTGACCTGT CATTCCACGA TTCCCTGGCC 
ACTTTCATTG CCATACTGAT TGCCCGGCAG TGCTTTTCTC TGGAAGATGT CGTTCAGCAC 
GTAGCACTGC CTTCTCTTCT TGCAGCTGGA AAATTATTTC CTGGAATTCG TTCTTCTTGT 
GATCGTCACC TCTTGGCTGC TGCTCACAAC AGCATAGAAG TGGGAGCTGT GTTTGCAGTC 
TTAAAAGCAA TTTTGATGCT TGGTGACGCT GAGATCGGCA GCAGCAGTGT CACCCCTGTG 
AAGAACGAGG AGCTGCCCGT GAGGGGCTTG CTGGATGAGA TCGCTGAGGA TGAAATGTGG 
GCTTCCTCTC ACGCTCTGAA ATCACGTGGA AAAGCTGTTT CCATAGAAAC CGCCAGTTTG 
AGCGAGTATG CTAGATATGT GCTAAGGACC ATTTGCCAGC AGGAATGGGT TGGAGAGCAC 
TGTTTAAAAG AACCTGAGAG GCTGTGCACA GATAAAGATC TAATTTTGGA TCCCGTTCTT 
TCAAACAAGC AAGCACAGAA ACTTCTTCAG CTTATCTGTT ATCCTCATGG TGTGAAAGAA 
TGCTCCGAGG GGGACAACCC TCAGAGGCAG CACATCAAAC GCATACTTCA GAACTTAGAC 
CAGTGGACTC TGCGGCAGTC TTGGTTGGAG CTGCAACTAA TGATCAAGCA GTGCATGAAG 
GAGCCTGGTT CTGGGTCTGT GGCTGAAATG AACAGCTTGT TGGATAATAT TGCAAAGGCG 
ACAATAGAGG TGTTTCAGCA ATCCGCTGAT CTAAACAATA ACTCTTCTAA CTCTGGTATA 
GGCCTCTTCA ATCCAAACTC TGTTGGAAAT GCTGACACAA GTAATACAAG ACAGAATGGT 
AAAAAGACAT TCCTAAGTTC CTCCGAGTGG CGAGGAGTGT GGCTGGTGGC TCCCTTGATT 
GCAAAACTAC CTACCTCAGT TCAAGGTAGG GTCTTGAAAG CTGCGGGAGA GGAGCTGGAG 
AAAGGTCAGC ATTTGGGGTC ATCTTCTAAG AAGGAAAGAG ATAGACAGAA GCAAAAGAGC 
ATGTCTCTCT TGAGTCAGCA GCCTTTCCTT TCTTTGGTAC TTACTTGCCT TAAGGGACAG 
GATGAGCAGC GGGAAGGGCT TCTAACATCA CTGCAGAATC AAGTCAATCA GATCCTTAGT 
AACTGGAGGG AAGAACGGTA CCAGGGTGAT GTTAAAGCTA GGCAAATGAT GCATGAAGCC 
TTACAGCTTC GTCTGAATTT GGTAGGTGGC ATGTTTGATA CTGTGCAGAG GAGTACCCAG 
TGGACCACAG ACTGGGCACT TCTGCTCCTC CAGATAATCA CTTCGGGAAC AGTTGATATG 
CAGACCAACA ACGAATTGTT CACAACCGTG CTGGATATGC TGGGTGTCCT CATCAACGGG 
ACGCTCGCCT CCGACTTGTC CAGTGCTTCC CAAGGGGGAC CCGAGGAAAA CAAAAGGGCT 
TACATGAACT TAGTGAAAAA GTTAAAAAAG GAGCTGGGAG ACAAGCGGTC AGACAGCATT 
GACAAGGTTC GCCAGCTGCT CCCTTTGCCA AAGCAGACAT GTGATATTAT TACCTGTGAG 
CCAATGGGAT CCTTGATTGA CACCAAGGGC AACAAAATTG CGGGGTTTGA CTCCATTGAT 
AAGAAACAGG GTCTTCAGGT GTCAACAAAA CAAAAGGTGT CCCCTTGGGA TTTGTTTGAA 
GGTCATAAAA ATCCTGCTCC TCTCTCCTGG GCCTGGTTTG GTACAGTTCG TGTCGACAGG 
AAGGTGATCA AATACGAAGA GCAGCAGCAT CTGCTCCTGT ACCATGCGCA CCCCAAACCC 
AAGCCGCGGA GCTACTACCT CGAGCCCCTG CCTCTGCCTC CGGAGGAGGA GGAGGAGGAG 
GAGCCCACCA CCCCTGTCTC TCAGGAACCA GAAAGGAAGT CAGGAGATCA GGATCAGGGA 
AAGCATGCCA CAGATGACGA GAAGAAGACA AAGGGCAGGA AGAGGAAGTC AAAGTCAGGC 
TCTAAGGCTG ATGAATATCC ACAGAACAAC TTATACAGAG TGCCTCCCAA TTACTCTCCC 
ATTCCCTCAC AAATGATGCA GCATCCTCAG TCTGCCCTGT GGGGCTACAC CATCATGGGG 
CAGCCGCAGC AGCCTGGCTT CTTCGTGCAG AACCAGCCCC TCCCTCCAGG TGGTTCCAGG 
CTGGATCCAA CGAGCTCCTT CGTCCCAGCC AACACGAAGC AGGCCCTGTC CAACATGCTG 
CAGCGGCGCT CCGGCACCGT GCTGCAGCCC CCTGCCCTCC ACGCAGTCAC ACCTCAGCAG 
CAGTTGCTCC AGATGAAACT CCTGCAGCAA GAGCAGCAGC AGCAGCGGCT CCTCAGGCAC 
CAAGCACAGG CACGGTCTCT CCAGCAGGAT GTCAGTAGCT CTGCCGTATC CCTTCAGGAT 
GGCTTTGACA ACATAATGGG TCAGCCGATG GACCCCGCTG CTCTTTTCAC CCCGCAAGTC 
CGACCCACGC CACAGCTACC GCAGTACCCA GGGCTTCAGC AAGCACAGAG CATGCCCCAC 
GGGTACACGA TGTACAGCAC CCCGATGCCT TTGCAGCAGC AGCAGCAGCC CAGCAGCGTG 
GTGCTCTCTC CTGGCTACAC CCCGCGGGCA TACACAGCCC ACTCCAGCCC CGTGCTGATG 
GAGCGGCTGC GGCCGGGGCA GCCGGGCAGC GTGGCTCAGC AGCAGGCAGC TGCCTACATG 
CAGCCCCTGG CGGGTGCTCA GCGGTTGACC CATCAGCCTC TGCAGCCAAA CTCATTGATA 
GGGGCAGGCC TGGACAGTGC ATCAACCGCA CGTACACATC CAACCTTGAG CTCAGCACAG 
CTGCCCCAGG AGCAAATGCG ACAGAGGCAG CAGGAGATCC GACAGCAGAG ACTCTTCCAG 
ATGCAGCAGG GGCAGCAGCA GCAGACTCTT GATCTTCAGC CTGTGCAGCC ACAGCAGCCC 
CTGGTAAGTG GGATGGATGG TGAGCGTGGG GCAGGGGCGT GGGATGAGCG GGTGAAGCTT 
ACAGTGAATT TGCAGTGCTG TTGTCCATGT TTCCTGGACA TGGCCATTTT TTCCAGATAT 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_015291833.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147319.1 Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X6, mRNA.

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CloneID	OGa40172
Clone ID Related Accession (Same CDS sequence)	XM_015291833.1
Accession Version	XM_015291833.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGAT TTTTTCTCCG 
ATGCCTGGGG AGTCCTGTGA GAACATGAAC TCTTCTTTAG ACAGAAGAAT TTCTGTTGCC 
AGTGAGAAGT CAGTCAAGAG GGAAAGACTC AGAGAAGTGA TTTTTCCATC TAACTATGAC 
CTCCTTCGTC ATCTGCAATA TGCAACGCAT TTCCCAATAC CTCTGGATGA ATCCTCAAGC 
CATGAGTGCA ACCAGCGCAT GATTCTTCTC TATGGTGTTG GCAAGGAGCG TGATGAAGCA 
AGACATCAGC TGAAGAAGAT TACCAAAGAC ATCCTGAAGA TTCTGAATAA GAAGAGTACT 
ACTGAAACGG GTGTTGGGGA TGAAGGGCAA AAAGCCAGGA AGAACAAACA AGAAGCATTT 
CCAACTCTAG AGACTGTGTT CACAAAACTA CAGCAGCTGT CATATTTTGA CCAACATCAG 
GTGACATCTC AGATCTCCAG CAATGTTCTC GAACAAATAA CCAGCTTTGC CTCAGGAACA 
TCCTATCACC TCCCCTTGGC TCACCACATA CAGCTCATCT TTGATCTCAT GGAACCAGCA 
CTGAATATCA ACGGGCTGAT AGACTTTGCA ATACAGCTGC TGAATGAGCT GAGCGTGGTG 
GAGGCAGAAC TGCTGTTGAA ATCCTCCGAC CTTGTTGGCA GCTACACCAC CAGCTTGTGC 
CTGTGCATCG TTGCCGTCCT GCGGCGTTAC CACGCCTGCC TCATTCTCAA CTCGGAGCAA 
ACTGCACAAG TCTTTGAAGG GTTGTGTGGA GTGGTGAAGC ACGTTGTTAA TCCCTCAGAG 
TGTTCCTCCC CAGAAAGATG TATTTTGGCC TACCTCTATG ACCTGTATGT GTCCTGCAGC 
CACCTGCGAA GCAAGTTTGG AGACCTCTTC AGTAGTGCCT GTTCTAAGGT GAAGCAAACG 
ATATACAGCA ATGTTCAGCC TTCAAATTCC AATCTGCTGT GGGACCCGGA GTTCATGCTT 
GATTTTATTG AGAACCCTTC TGCTCACAGC ATTAACTACT CCATGCTGGG CAAGATCCTG 
AGTGACAACG CAGCCAACCG CTACAGCTTT GTCTGCAATG CTCTAATGAA TGTGTGCATG 
GGGCACCAAG ATGCAGGGAG GATTAATGAC ATAGCAAACC TTTGTGCAGA GCTGACGGCG 
TGCTGCACCG TGCTGAGCTC GGAGTGGCTG GGGGTTGTCA AAGCTCTTTG CTGCTCTTCC 
AATCACGTGT GGGGTTTCAA TGATCTGCTC TGCAGCGTGG ATGTGAGTGA CCTGTCATTC 
CACGATTCCC TGGCCACTTT CATTGCCATA CTGATTGCCC GGCAGTGCTT TTCTCTGGAA 
GATGTCGTTC AGCACGTAGC ACTGCCTTCT CTTCTTGCAG CTGCCTGTGG GGACCCAGAT 
GCTGAGCCTG GGGCCAGGAT GACTTGTCGG CTTCTCCTGC ACCTCTTCAG GACACCCCAG 
GTCTGCCTCT TTCCCCAGGG AGCAGGAAAA TTATTTCCTG GAATTCGTTC TTCTTGTGAT 
CGTCACCTCT TGGCTGCTGC TCACAACAGC ATAGAAGTGG GAGCTGTGTT TGCAGTCTTA 
AAAGCAATTT TGATGCTTGG TGACGCTGAG ATCGGCAGCA GCAGTGTCAC CCCTGTGAAG 
AACGAGGAGC TGCCCGTGAG GGGCTTGCTG GATGAGATCG CTGAGGATGA AATGTGGGCT 
TCCTCTCACG CTCTGAAATC ACGTGGAAAA GCTGTTTCCA TAGAAACCGC CAGTTTGAGC 
GAGTATGCTA GATATGTGCT AAGGACCATT TGCCAGCAGG AATGGGTTGG AGAGCACTGT 
TTAAAAGAAC CTGAGAGGCT GTGCACAGAT AAAGATCTAA TTTTGGATCC CGTTCTTTCA 
AACAAGCAAG CACAGAAACT TCTTCAGCTT ATCTGTTATC CTCATGGTGT GAAAGAATGC 
TCCGAGGGGG ACAACCCTCA GAGGCAGCAC ATCAAACGCA TACTTCAGAA CTTAGACCAG 
TGGACTCTGC GGCAGTCTTG GTTGGAGCTG CAACTAATGA TCAAGCAGTG CATGAAGGAG 
CCTGGTTCTG GGTCTGTGGC TGAAATGAAC AGCTTGTTGG ATAATATTGC AAAGGCGACA 
ATAGAGGTGT TTCAGCAATC CGCTGATCTA AACAATAACT CTTCTAACTC TGGTATAGGC 
CTCTTCAATC CAAACTCTGT TGGAAATGCT GACACAAGTA ATACAAGACA GAATGGTAAA 
AAGACATTCC TAAGTTCCTC CGAGTGGCGA GGAGTGTGGC TGGTGGCTCC CTTGATTGCA 
AAACTACCTA CCTCAGTTCA AGGTAGGGTC TTGAAAGCTG CGGGAGAGGA GCTGGAGAAA 
GGTCAGCATT TGGGGTCATC TTCTAAGAAG GAAAGAGATA GACAGAAGCA AAAGAGCATG 
TCTCTCTTGA GTCAGCAGCC TTTCCTTTCT TTGGTACTTA CTTGCCTTAA GGGACAGGAT 
GAGCAGCGGG AAGGGCTTCT AACATCACTG CAGAATCAAG TCAATCAGAT CCTTAGTAAC 
TGGAGGGAAG AACGGTACCA GGGTGATGTT AAAGCTAGGC AAATGATGCA TGAAGCCTTA 
CAGCTTCGTC TGAATTTGGT AGGTGGCATG TTTGATACTG TGCAGAGGAG TACCCAGTGG 
ACCACAGACT GGGCACTTCT GCTCCTCCAG ATAATCACTT CGGGAACAGT TGATATGCAG 
ACCAACAACG AATTGTTCAC AACCGTGCTG GATATGCTGG GTGTCCTCAT CAACGGGACG 
CTCGCCTCCG ACTTGTCCAG TGCTTCCCAA GGGGGACCCG AGGAAAACAA AAGGGCTTAC 
ATGAACTTAG TGAAAAAGTT AAAAAAGGAG CTGGGAGACA AGCGGTCAGA CAGCATTGAC 
AAGGTTCGCC AGCTGCTCCC TTTGCCAAAG CAGACATGTG ATATTATTAC CTGTGAGCCA 
ATGGGATCCT TGATTGACAC CAAGGGCAAC AAAATTGCGG GGTTTGACTC CATTGATAAG 
AAACAGGGTC TTCAGGTGTC AACAAAACAA AAGGTGTCCC CTTGGGATTT GTTTGAAGGT 
CATAAAAATC CTGCTCCTCT CTCCTGGGCC TGGTTTGGTA CAGTTCGTGT CGACAGGAAG 
GTGATCAAAT ACGAAGAGCA GCAGCATCTG CTCCTGTACC ATGCGCACCC CAAACCCAAG 
CCGCGGAGCT ACTACCTCGA GCCCCTGCCT CTGCCTCCGG AGGAGGAGGA GGAGGAGGAG 
CCCACCACCC CTGTCTCTCA GGAACCAGAA AGGAAGTCAG GAGATCAGGA TCAGGGAAAG 
CATGCCACAG ATGACGAGAA GAAGACAAAG GGCAGGAAGA GGAAGTCAAA GTCAGGCTCT 
AAGGCTGATG AATATCCACA GAACAACTTA TACAGAGTGC CTCCCAATTA CTCTCCCATT 
CCCTCACAAA TGATGCAGCA TCCTCAGTCT GCCCTGTGGG GCTACACCAT CATGGGGCAG 
CCGCAGCAGC CTGGCTTCTT CGTGCAGAAC CAGCCCCTCC CTCCAGGTGG TTCCAGGCTG 
GATCCAACGA GCTCCTTCGT CCCAGCCAAC ACGAAGCAGG CCCTGTCCAA CATGCTGCAG 
CGGCGCTCCG GCACCGTGCT GCAGCCCCCT GCCCTCCACG CAGTCACACC TCAGCAGCAG 
TTGCTCCAGA TGAAACTCCT GCAGCAAGAG CAGCAGCAGC AGCGGCTCCT CAGGCACCAA 
GCACAGGCAC GGTCTCTCCA GCAGGATGTC AGTAGCTCTG CCGTATCCCT TCAGGATGGC 
TTTGACAACA TAATGGGTCA GCCGATGGAC CCCGCTGCTC TTTTCACCCC GCAAGTCCGA 
CCCACGCCAC AGCTACCGCA GTACCCAGGG CTTCAGCAAG CACAGAGCAT GCCCCACGGG 
TACACGATGT ACAGCACCCC GATGCCTTTG CAGCAGCAGC AGCAGCCCAG CAGCGTGGTG 
CTCTCTCCTG GCTACACCCC GCGGGCATAC ACAGCCCACT CCAGCCCCGT GCTGATGGAG 
CGGCTGCGGC CGGGGCAGCC GGGCAGCGTG GCTCAGCAGC AGGCAGCTGC CTACATGCAG 
CCCCTGGCGG GTGCTCAGCG GTTGACCCAT CAGCCTCTGC AGCCAAACTC ATTGATAGGG 
GCAGGCCTGG ACAGTGCATC AACCGCACGT ACACATCCAA CCTTGAGCTC AGCACAGCTG 
CCCCAGGAGC AAATGCGACA GAGGCAGCAG GAGATCCGAC AGCAGAGACT CTTCCAGATG 
CAGCAGGGGC AGCAGCAGCA GACTCTTGAT CTTCAGCCTG TGCAGCCACA GCAGCCCCTG 
GTAAGTGGGA TGGATGGTGA GCGTGGGGCA GGGGCGTGGG ATGAGCGGGT GAAGCTTACA 
GTGAATTTGC AGTGCTGTTG TCCATGTTTC CTGGACATGG CCATTTTTTC CAGATATGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147319.1
CDS	141..6563
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPRQKEDELTAVNVKQGFSNQPAFSGDEHGSARNIVINASKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHNWFTDLAGSKPLTILAKKVPILSKKEDVFAYLAKYSVPLLRAAWLIKMTCAYYAAISEAKIKKRQATDPNIEWTQIITRYLREQLAKVAEFYHVTSSQGNSSVVMPQEMEQALKQWEYNEKLSFYMFQEGMLERHEYLTWILDVLEKIRPTDDDILKLVLPLMLQYSEEFVQSAYLSRRLAYFCARRLSLLLSDGPSLAAAHSPHVIIAPSNPPLAVPSPTAPGPVLSPVQLTCSDFLSCPQHRPLVYGLSCMLQTITLCCPSALVWNYSTNENKNMNPGSPLDLLQVAPSSLPMPGGNSTFNQQVRAKIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRSDSSNSMETLYHKIFWANQNKDNQEAAPSDEAVVMLLCEWAVSCKRSGKHRAMVVAKLLEKRQAEIEAERCGEAEVLDEKESLSSASLTSSSLPVFQNILLRFLDTQAPSLSDPNSDHEKTEFVNLVLLFSEFTRHDVFSHDAYMCTLISRGDLSITATTRPRSPNGETGDEHYSKDHDMKMEIFSPMPGESCENMNSSLDRRISVASEKSVKRERLREVIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRMILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQEAFPTLETVFTKLQQLSYFDQHQVTSQISSNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRRYHACLILNSEQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYSNVQPSNSNLLWDPEFMLDFIENPSAHSINYSMLGKILSDNAANRYSFVCNALMNVCMGHQDAGRINDIANLCAELTACCTVLSSEWLGVVKALCCSSNHVWGFNDLLCSVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDPDAEPGARMTCRLLLHLFRTPQVCLFPQGAGKLFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAILMLGDAEIGSSSVTPVKNEELPVRGLLDEIAEDEMWASSHALKSRGKAVSIETASLSEYARYVLRTICQQEWVGEHCLKEPERLCTDKDLILDPVLSNKQAQKLLQLICYPHGVKECSEGDNPQRQHIKRILQNLDQWTLRQSWLELQLMIKQCMKEPGSGSVAEMNSLLDNIAKATIEVFQQSADLNNNSSNSGIGLFNPNSVGNADTSNTRQNGKKTFLSSSEWRGVWLVAPLIAKLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQGDVKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMQTNNELFTTVLDMLGVLINGTLASDLSSASQGGPEENKRAYMNLVKKLKKELGDKRSDSIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGHKNPAPLSWAWFGTVRVDRKVIKYEEQQHLLLYHAHPKPKPRSYYLEPLPLPPEEEEEEEPTTPVSQEPERKSGDQDQGKHATDDEKKTKGRKRKSKSGSKADEYPQNNLYRVPPNYSPIPSQMMQHPQSALWGYTIMGQPQQPGFFVQNQPLPPGGSRLDPTSSFVPANTKQALSNMLQRRSGTVLQPPALHAVTPQQQLLQMKLLQQEQQQQRLLRHQAQARSLQQDVSSSAVSLQDGFDNIMGQPMDPAALFTPQVRPTPQLPQYPGLQQAQSMPHGYTMYSTPMPLQQQQQPSSVVLSPGYTPRAYTAHSSPVLMERLRPGQPGSVAQQQAAAYMQPLAGAQRLTHQPLQPNSLIGAGLDSASTARTHPTLSSAQLPQEQMRQRQQEIRQQRLFQMQQGQQQQTLDLQPVQPQQPLVSGMDGERGAGAWDERVKLTVNLQCCCPCFLDMAIFSRYV

Target ORF information:

RefSeq Version	XM_015291833.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291833.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGAT TTTTTCTCCG 
ATGCCTGGGG AGTCCTGTGA GAACATGAAC TCTTCTTTAG ACAGAAGAAT TTCTGTTGCC 
AGTGAGAAGT CAGTCAAGAG GGAAAGACTC AGAGAAGTGA TTTTTCCATC TAACTATGAC 
CTCCTTCGTC ATCTGCAATA TGCAACGCAT TTCCCAATAC CTCTGGATGA ATCCTCAAGC 
CATGAGTGCA ACCAGCGCAT GATTCTTCTC TATGGTGTTG GCAAGGAGCG TGATGAAGCA 
AGACATCAGC TGAAGAAGAT TACCAAAGAC ATCCTGAAGA TTCTGAATAA GAAGAGTACT 
ACTGAAACGG GTGTTGGGGA TGAAGGGCAA AAAGCCAGGA AGAACAAACA AGAAGCATTT 
CCAACTCTAG AGACTGTGTT CACAAAACTA CAGCAGCTGT CATATTTTGA CCAACATCAG 
GTGACATCTC AGATCTCCAG CAATGTTCTC GAACAAATAA CCAGCTTTGC CTCAGGAACA 
TCCTATCACC TCCCCTTGGC TCACCACATA CAGCTCATCT TTGATCTCAT GGAACCAGCA 
CTGAATATCA ACGGGCTGAT AGACTTTGCA ATACAGCTGC TGAATGAGCT GAGCGTGGTG 
GAGGCAGAAC TGCTGTTGAA ATCCTCCGAC CTTGTTGGCA GCTACACCAC CAGCTTGTGC 
CTGTGCATCG TTGCCGTCCT GCGGCGTTAC CACGCCTGCC TCATTCTCAA CTCGGAGCAA 
ACTGCACAAG TCTTTGAAGG GTTGTGTGGA GTGGTGAAGC ACGTTGTTAA TCCCTCAGAG 
TGTTCCTCCC CAGAAAGATG TATTTTGGCC TACCTCTATG ACCTGTATGT GTCCTGCAGC 
CACCTGCGAA GCAAGTTTGG AGACCTCTTC AGTAGTGCCT GTTCTAAGGT GAAGCAAACG 
ATATACAGCA ATGTTCAGCC TTCAAATTCC AATCTGCTGT GGGACCCGGA GTTCATGCTT 
GATTTTATTG AGAACCCTTC TGCTCACAGC ATTAACTACT CCATGCTGGG CAAGATCCTG 
AGTGACAACG CAGCCAACCG CTACAGCTTT GTCTGCAATG CTCTAATGAA TGTGTGCATG 
GGGCACCAAG ATGCAGGGAG GATTAATGAC ATAGCAAACC TTTGTGCAGA GCTGACGGCG 
TGCTGCACCG TGCTGAGCTC GGAGTGGCTG GGGGTTGTCA AAGCTCTTTG CTGCTCTTCC 
AATCACGTGT GGGGTTTCAA TGATCTGCTC TGCAGCGTGG ATGTGAGTGA CCTGTCATTC 
CACGATTCCC TGGCCACTTT CATTGCCATA CTGATTGCCC GGCAGTGCTT TTCTCTGGAA 
GATGTCGTTC AGCACGTAGC ACTGCCTTCT CTTCTTGCAG CTGCCTGTGG GGACCCAGAT 
GCTGAGCCTG GGGCCAGGAT GACTTGTCGG CTTCTCCTGC ACCTCTTCAG GACACCCCAG 
GTCTGCCTCT TTCCCCAGGG AGCAGGAAAA TTATTTCCTG GAATTCGTTC TTCTTGTGAT 
CGTCACCTCT TGGCTGCTGC TCACAACAGC ATAGAAGTGG GAGCTGTGTT TGCAGTCTTA 
AAAGCAATTT TGATGCTTGG TGACGCTGAG ATCGGCAGCA GCAGTGTCAC CCCTGTGAAG 
AACGAGGAGC TGCCCGTGAG GGGCTTGCTG GATGAGATCG CTGAGGATGA AATGTGGGCT 
TCCTCTCACG CTCTGAAATC ACGTGGAAAA GCTGTTTCCA TAGAAACCGC CAGTTTGAGC 
GAGTATGCTA GATATGTGCT AAGGACCATT TGCCAGCAGG AATGGGTTGG AGAGCACTGT 
TTAAAAGAAC CTGAGAGGCT GTGCACAGAT AAAGATCTAA TTTTGGATCC CGTTCTTTCA 
AACAAGCAAG CACAGAAACT TCTTCAGCTT ATCTGTTATC CTCATGGTGT GAAAGAATGC 
TCCGAGGGGG ACAACCCTCA GAGGCAGCAC ATCAAACGCA TACTTCAGAA CTTAGACCAG 
TGGACTCTGC GGCAGTCTTG GTTGGAGCTG CAACTAATGA TCAAGCAGTG CATGAAGGAG 
CCTGGTTCTG GGTCTGTGGC TGAAATGAAC AGCTTGTTGG ATAATATTGC AAAGGCGACA 
ATAGAGGTGT TTCAGCAATC CGCTGATCTA AACAATAACT CTTCTAACTC TGGTATAGGC 
CTCTTCAATC CAAACTCTGT TGGAAATGCT GACACAAGTA ATACAAGACA GAATGGTAAA 
AAGACATTCC TAAGTTCCTC CGAGTGGCGA GGAGTGTGGC TGGTGGCTCC CTTGATTGCA 
AAACTACCTA CCTCAGTTCA AGGTAGGGTC TTGAAAGCTG CGGGAGAGGA GCTGGAGAAA 
GGTCAGCATT TGGGGTCATC TTCTAAGAAG GAAAGAGATA GACAGAAGCA AAAGAGCATG 
TCTCTCTTGA GTCAGCAGCC TTTCCTTTCT TTGGTACTTA CTTGCCTTAA GGGACAGGAT 
GAGCAGCGGG AAGGGCTTCT AACATCACTG CAGAATCAAG TCAATCAGAT CCTTAGTAAC 
TGGAGGGAAG AACGGTACCA GGGTGATGTT AAAGCTAGGC AAATGATGCA TGAAGCCTTA 
CAGCTTCGTC TGAATTTGGT AGGTGGCATG TTTGATACTG TGCAGAGGAG TACCCAGTGG 
ACCACAGACT GGGCACTTCT GCTCCTCCAG ATAATCACTT CGGGAACAGT TGATATGCAG 
ACCAACAACG AATTGTTCAC AACCGTGCTG GATATGCTGG GTGTCCTCAT CAACGGGACG 
CTCGCCTCCG ACTTGTCCAG TGCTTCCCAA GGGGGACCCG AGGAAAACAA AAGGGCTTAC 
ATGAACTTAG TGAAAAAGTT AAAAAAGGAG CTGGGAGACA AGCGGTCAGA CAGCATTGAC 
AAGGTTCGCC AGCTGCTCCC TTTGCCAAAG CAGACATGTG ATATTATTAC CTGTGAGCCA 
ATGGGATCCT TGATTGACAC CAAGGGCAAC AAAATTGCGG GGTTTGACTC CATTGATAAG 
AAACAGGGTC TTCAGGTGTC AACAAAACAA AAGGTGTCCC CTTGGGATTT GTTTGAAGGT 
CATAAAAATC CTGCTCCTCT CTCCTGGGCC TGGTTTGGTA CAGTTCGTGT CGACAGGAAG 
GTGATCAAAT ACGAAGAGCA GCAGCATCTG CTCCTGTACC ATGCGCACCC CAAACCCAAG 
CCGCGGAGCT ACTACCTCGA GCCCCTGCCT CTGCCTCCGG AGGAGGAGGA GGAGGAGGAG 
CCCACCACCC CTGTCTCTCA GGAACCAGAA AGGAAGTCAG GAGATCAGGA TCAGGGAAAG 
CATGCCACAG ATGACGAGAA GAAGACAAAG GGCAGGAAGA GGAAGTCAAA GTCAGGCTCT 
AAGGCTGATG AATATCCACA GAACAACTTA TACAGAGTGC CTCCCAATTA CTCTCCCATT 
CCCTCACAAA TGATGCAGCA TCCTCAGTCT GCCCTGTGGG GCTACACCAT CATGGGGCAG 
CCGCAGCAGC CTGGCTTCTT CGTGCAGAAC CAGCCCCTCC CTCCAGGTGG TTCCAGGCTG 
GATCCAACGA GCTCCTTCGT CCCAGCCAAC ACGAAGCAGG CCCTGTCCAA CATGCTGCAG 
CGGCGCTCCG GCACCGTGCT GCAGCCCCCT GCCCTCCACG CAGTCACACC TCAGCAGCAG 
TTGCTCCAGA TGAAACTCCT GCAGCAAGAG CAGCAGCAGC AGCGGCTCCT CAGGCACCAA 
GCACAGGCAC GGTCTCTCCA GCAGGATGTC AGTAGCTCTG CCGTATCCCT TCAGGATGGC 
TTTGACAACA TAATGGGTCA GCCGATGGAC CCCGCTGCTC TTTTCACCCC GCAAGTCCGA 
CCCACGCCAC AGCTACCGCA GTACCCAGGG CTTCAGCAAG CACAGAGCAT GCCCCACGGG 
TACACGATGT ACAGCACCCC GATGCCTTTG CAGCAGCAGC AGCAGCCCAG CAGCGTGGTG 
CTCTCTCCTG GCTACACCCC GCGGGCATAC ACAGCCCACT CCAGCCCCGT GCTGATGGAG 
CGGCTGCGGC CGGGGCAGCC GGGCAGCGTG GCTCAGCAGC AGGCAGCTGC CTACATGCAG 
CCCCTGGCGG GTGCTCAGCG GTTGACCCAT CAGCCTCTGC AGCCAAACTC ATTGATAGGG 
GCAGGCCTGG ACAGTGCATC AACCGCACGT ACACATCCAA CCTTGAGCTC AGCACAGCTG 
CCCCAGGAGC AAATGCGACA GAGGCAGCAG GAGATCCGAC AGCAGAGACT CTTCCAGATG 
CAGCAGGGGC AGCAGCAGCA GACTCTTGAT CTTCAGCCTG TGCAGCCACA GCAGCCCCTG 
GTAAGTGGGA TGGATGGTGA GCGTGGGGCA GGGGCGTGGG ATGAGCGGGT GAAGCTTACA 
GTGAATTTGC AGTGCTGTTG TCCATGTTTC CTGGACATGG CCATTTTTTC CAGATATGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_015291834.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147320.1 Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X7, mRNA.

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CloneID	OGa40173
Clone ID Related Accession (Same CDS sequence)	XM_015291834.1
Accession Version	XM_015291834.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAACTGCTG 
TTGAAATCCT CCGACCTTGT TGGCAGCTAC ACCACCAGCT TGTGCCTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCT AAGGTGAAGC AAACGATATA CAGCAATGTT 
CAGCCTTCAA ATTCCAATCT GCTGTGGGAC CCGGAGTTCA TGCTTGATTT TATTGAGAAC 
CCTTCTGCTC ACAGCATTAA CTACTCCATG CTGGGCAAGA TCCTGAGTGA CAACGCAGCC 
AACCGCTACA GCTTTGTCTG CAATGCTCTA ATGAATGTGT GCATGGGGCA CCAAGATGCA 
GGGAGGATTA ATGACATAGC AAACCTTTGT GCAGAGCTGA CGGCGTGCTG CACCGTGCTG 
AGCTCGGAGT GGCTGGGGGT TGTCAAAGCT CTTTGCTGCT CTTCCAATCA CGTGTGGGGT 
TTCAATGATC TGCTCTGCAG CGTGGATGTG AGTGACCTGT CATTCCACGA TTCCCTGGCC 
ACTTTCATTG CCATACTGAT TGCCCGGCAG TGCTTTTCTC TGGAAGATGT CGTTCAGCAC 
GTAGCACTGC CTTCTCTTCT TGCAGCTGCC TGTGGGGACC CAGATGCTGA GCCTGGGGCC 
AGGATGACTT GTCGGCTTCT CCTGCACCTC TTCAGGACAC CCCAGGTCTG CCTCTTTCCC 
CAGGGAGCAG GAAAATTATT TCCTGGAATT CGTTCTTCTT GTGATCGTCA CCTCTTGGCT 
GCTGCTCACA ACAGCATAGA AGTGGGAGCT GTGTTTGCAG TCTTAAAAGC AATTTTGATG 
CTTGGTGACG CTGAGATCGG CAGCAGCAGT GTCACCCCTG TGAAGAACGA GGAGCTGCCC 
GTGAGGGGCT TGCTGGATGA GATCGCTGAG GATGAAATGT GGGCTTCCTC TCACGCTCTG 
AAATCACGTG GAAAAGCTGT TTCCATAGAA ACCGCCAGTT TGAGCGAGTA TGCTAGATAT 
GTGCTAAGGA CCATTTGCCA GCAGGAATGG GTTGGAGAGC ACTGTTTAAA AGAACCTGAG 
AGGCTGTGCA CAGATAAAGA TCTAATTTTG GATCCCGTTC TTTCAAACAA GCAAGCACAG 
AAACTTCTTC AGCTTATCTG TTATCCTCAT GGTGTGAAAG AATGCTCCGA GGGGGACAAC 
CCTCAGAGGC AGCACATCAA ACGCATACTT CAGAACTTAG ACCAGTGGAC TCTGCGGCAG 
TCTTGGTTGG AGCTGCAACT AATGATCAAG CAGTGCATGA AGGAGCCTGG TTCTGGGTCT 
GTGGCTGAAA TGAACAGCTT GTTGGATAAT ATTGCAAAGG CGACAATAGA GGTGTTTCAG 
CAATCCGCTG ATCTAAACAA TAACTCTTCT AACTCTGGTA TAGGCCTCTT CAATCCAAAC 
TCTGTTGGAA ATGCTGACAC AAGTAATACA AGACAGAATG GTAAAAAGAC ATTCCTAAGT 
TCCTCCGAGT GGCGAGGAGT GTGGCTGGTG GCTCCCTTGA TTGCAAAACT ACCTACCTCA 
GTTCAAGGTA GGGTCTTGAA AGCTGCGGGA GAGGAGCTGG AGAAAGGTCA GCATTTGGGG 
TCATCTTCTA AGAAGGAAAG AGATAGACAG AAGCAAAAGA GCATGTCTCT CTTGAGTCAG 
CAGCCTTTCC TTTCTTTGGT ACTTACTTGC CTTAAGGGAC AGGATGAGCA GCGGGAAGGG 
CTTCTAACAT CACTGCAGAA TCAAGTCAAT CAGATCCTTA GTAACTGGAG GGAAGAACGG 
TACCAGGGTG ATGTTAAAGC TAGGCAAATG ATGCATGAAG CCTTACAGCT TCGTCTGAAT 
TTGGTAGGTG GCATGTTTGA TACTGTGCAG AGGAGTACCC AGTGGACCAC AGACTGGGCA 
CTTCTGCTCC TCCAGATAAT CACTTCGGGA ACAGTTGATA TGCAGACCAA CAACGAATTG 
TTCACAACCG TGCTGGATAT GCTGGGTGTC CTCATCAACG GGACGCTCGC CTCCGACTTG 
TCCAGTGCTT CCCAAGGGGG ACCCGAGGAA AACAAAAGGG CTTACATGAA CTTAGTGAAA 
AAGTTAAAAA AGGAGCTGGG AGACAAGCGG TCAGACAGCA TTGACAAGGT TCGCCAGCTG 
CTCCCTTTGC CAAAGCAGAC ATGTGATATT ATTACCTGTG AGCCAATGGG ATCCTTGATT 
GACACCAAGG GCAACAAAAT TGCGGGGTTT GACTCCATTG ATAAGAAACA GGGTCTTCAG 
GTGTCAACAA AACAAAAGGT GTCCCCTTGG GATTTGTTTG AAGGTCATAA AAATCCTGCT 
CCTCTCTCCT GGGCCTGGTT TGGTACAGTT CGTGTCGACA GGAAGGTGAT CAAATACGAA 
GAGCAGCAGC ATCTGCTCCT GTACCATGCG CACCCCAAAC CCAAGCCGCG GAGCTACTAC 
CTCGAGCCCC TGCCTCTGCC TCCGGAGGAG GAGGAGGAGG AGGAGCCCAC CACCCCTGTC 
TCTCAGGAAC CAGAAAGGAA GTCAGGAGAT CAGGATCAGG GAAAGCATGC CACAGATGAC 
GAGAAGAAGA CAAAGGGCAG GAAGAGGAAG TCAAAGTCAG GCTCTAAGGC TGATGAATAT 
CCACAGAACA ACTTATACAG AGTGCCTCCC AATTACTCTC CCATTCCCTC ACAAATGATG 
CAGCATCCTC AGTCTGCCCT GTGGGGCTAC ACCATCATGG GGCAGCCGCA GCAGCCTGGC 
TTCTTCGTGC AGAACCAGCC CCTCCCTCCA GGTGGTTCCA GGCTGGATCC AACGAGCTCC 
TTCGTCCCAG CCAACACGAA GCAGGCCCTG TCCAACATGC TGCAGCGGCG CTCCGGCACC 
GTGCTGCAGC CCCCTGCCCT CCACGCAGTC ACACCTCAGC AGCAGTTGCT CCAGATGAAA 
CTCCTGCAGC AAGAGCAGCA GCAGCAGCGG CTCCTCAGGC ACCAAGCACA GGCACGGTCT 
CTCCAGCAGA GCATGCCCCA CGGGTACACG ATGTACAGCA CCCCGATGCC TTTGCAGCAG 
CAGCAGCAGC CCAGCAGCGT GGTGCTCTCT CCTGGCTACA CCCCGCGGGC ATACACAGCC 
CACTCCAGCC CCGTGCTGAT GGAGCGGCTG CGGCCGGGGC AGCCGGGCAG CGTGGCTCAG 
CAGCAGGCAG CTGCCTACAT GCAGCCCCTG GCGGGTGCTC AGCGGTTGAC CCATCAGCCT 
CTGCAGCCAA ACTCATTGAT AGGGGCAGGC CTGGACAGTG CATCAACCGC ACGTACACAT 
CCAACCTTGA GCTCAGCACA GCTGCCCCAG GAGCAAATGC GACAGAGGCA GCAGGAGATC 
CGACAGCAGA GACTCTTCCA GATGCAGCAG GGGCAGCAGC AGCAGACTCT TGATCTTCAG 
CCTGTGCAGC CACAGCAGCC CCTGGTAAGT GGGATGGATG GTGAGCGTGG GGCAGGGGCG 
TGGGATGAGC GGGTGAAGCT TACAGTGAAT TTGCAGTGCT GTTGTCCATG TTTCCTGGAC 
ATGGCCATTT TTTCCAGATA TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147320.1
CDS	143..6529
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPRQKEDELTAVNVKQGFSNQPAFSGDEHGSARNIVINASKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHNWFTDLAGSKPLTILAKKVPILSKKEDVFAYLAKYSVPLLRAAWLIKMTCAYYAAISEAKIKKRQATDPNIEWTQIITRYLREQLAKVAEFYHVTSSQGNSSVVMPQEMEQALKQWEYNEKLSFYMFQEGMLERHEYLTWILDVLEKIRPTDDDILKLVLPLMLQYSEEFVQSAYLSRRLAYFCARRLSLLLSDGPSLAAAHSPHVIIAPSNPPLAVPSPTAPGPVLSPVQLTCSDFLSCPQHRPLVYGLSCMLQTITLCCPSALVWNYSTNENKNMNPGSPLDLLQVAPSSLPMPGGNSTFNQQVRAKIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRSDSSNSMETLYHKIFWANQNKDNQEAAPSDEAVVMLLCEWAVSCKRSGKHRAMVVAKLLEKRQAEIEAERCGEAEVLDEKESLSSASLTSSSLPVFQNILLRFLDTQAPSLSDPNSDHEKTEFVNLVLLFSEFTRHDVFSHDAYMCTLISRGDLSITATTRPRSPNGETGDEHYSKDHDMKMEDHNIMEHMGTDSGPTSIFDDVDKGDFKADFGSEFPIFSPMPGESCENMNSSLDRRISVASEKSVKRERLREVIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRMILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQEAFPTLETVFTKLQQLSYFDQHQVTSQISSNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRRYHACLILNSEQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYSNVQPSNSNLLWDPEFMLDFIENPSAHSINYSMLGKILSDNAANRYSFVCNALMNVCMGHQDAGRINDIANLCAELTACCTVLSSEWLGVVKALCCSSNHVWGFNDLLCSVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDPDAEPGARMTCRLLLHLFRTPQVCLFPQGAGKLFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAILMLGDAEIGSSSVTPVKNEELPVRGLLDEIAEDEMWASSHALKSRGKAVSIETASLSEYARYVLRTICQQEWVGEHCLKEPERLCTDKDLILDPVLSNKQAQKLLQLICYPHGVKECSEGDNPQRQHIKRILQNLDQWTLRQSWLELQLMIKQCMKEPGSGSVAEMNSLLDNIAKATIEVFQQSADLNNNSSNSGIGLFNPNSVGNADTSNTRQNGKKTFLSSSEWRGVWLVAPLIAKLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQGDVKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMQTNNELFTTVLDMLGVLINGTLASDLSSASQGGPEENKRAYMNLVKKLKKELGDKRSDSIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGHKNPAPLSWAWFGTVRVDRKVIKYEEQQHLLLYHAHPKPKPRSYYLEPLPLPPEEEEEEEPTTPVSQEPERKSGDQDQGKHATDDEKKTKGRKRKSKSGSKADEYPQNNLYRVPPNYSPIPSQMMQHPQSALWGYTIMGQPQQPGFFVQNQPLPPGGSRLDPTSSFVPANTKQALSNMLQRRSGTVLQPPALHAVTPQQQLLQMKLLQQEQQQQRLLRHQAQARSLQQSMPHGYTMYSTPMPLQQQQQPSSVVLSPGYTPRAYTAHSSPVLMERLRPGQPGSVAQQQAAAYMQPLAGAQRLTHQPLQPNSLIGAGLDSASTARTHPTLSSAQLPQEQMRQRQQEIRQQRLFQMQQGQQQQTLDLQPVQPQQPLVSGMDGERGAGAWDERVKLTVNLQCCCPCFLDMAIFSRYV

Target ORF information:

RefSeq Version	XM_015291834.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291834.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGGA TCATAATATT 
ATGGAGCACA TGGGCACTGA CTCAGGACCC ACTAGTATCT TTGATGATGT AGACAAGGGT 
GACTTTAAGG CAGATTTTGG TTCGGAATTT CCAATTTTTT CTCCGATGCC TGGGGAGTCC 
TGTGAGAACA TGAACTCTTC TTTAGACAGA AGAATTTCTG TTGCCAGTGA GAAGTCAGTC 
AAGAGGGAAA GACTCAGAGA AGTGATTTTT CCATCTAACT ATGACCTCCT TCGTCATCTG 
CAATATGCAA CGCATTTCCC AATACCTCTG GATGAATCCT CAAGCCATGA GTGCAACCAG 
CGCATGATTC TTCTCTATGG TGTTGGCAAG GAGCGTGATG AAGCAAGACA TCAGCTGAAG 
AAGATTACCA AAGACATCCT GAAGATTCTG AATAAGAAGA GTACTACTGA AACGGGTGTT 
GGGGATGAAG GGCAAAAAGC CAGGAAGAAC AAACAAGAAG CATTTCCAAC TCTAGAGACT 
GTGTTCACAA AACTACAGCA GCTGTCATAT TTTGACCAAC ATCAGGTGAC ATCTCAGATC 
TCCAGCAATG TTCTCGAACA AATAACCAGC TTTGCCTCAG GAACATCCTA TCACCTCCCC 
TTGGCTCACC ACATACAGCT CATCTTTGAT CTCATGGAAC CAGCACTGAA TATCAACGGG 
CTGATAGACT TTGCAATACA GCTGCTGAAT GAGCTGAGCG TGGTGGAGGC AGAACTGCTG 
TTGAAATCCT CCGACCTTGT TGGCAGCTAC ACCACCAGCT TGTGCCTGTG CATCGTTGCC 
GTCCTGCGGC GTTACCACGC CTGCCTCATT CTCAACTCGG AGCAAACTGC ACAAGTCTTT 
GAAGGGTTGT GTGGAGTGGT GAAGCACGTT GTTAATCCCT CAGAGTGTTC CTCCCCAGAA 
AGATGTATTT TGGCCTACCT CTATGACCTG TATGTGTCCT GCAGCCACCT GCGAAGCAAG 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCT AAGGTGAAGC AAACGATATA CAGCAATGTT 
CAGCCTTCAA ATTCCAATCT GCTGTGGGAC CCGGAGTTCA TGCTTGATTT TATTGAGAAC 
CCTTCTGCTC ACAGCATTAA CTACTCCATG CTGGGCAAGA TCCTGAGTGA CAACGCAGCC 
AACCGCTACA GCTTTGTCTG CAATGCTCTA ATGAATGTGT GCATGGGGCA CCAAGATGCA 
GGGAGGATTA ATGACATAGC AAACCTTTGT GCAGAGCTGA CGGCGTGCTG CACCGTGCTG 
AGCTCGGAGT GGCTGGGGGT TGTCAAAGCT CTTTGCTGCT CTTCCAATCA CGTGTGGGGT 
TTCAATGATC TGCTCTGCAG CGTGGATGTG AGTGACCTGT CATTCCACGA TTCCCTGGCC 
ACTTTCATTG CCATACTGAT TGCCCGGCAG TGCTTTTCTC TGGAAGATGT CGTTCAGCAC 
GTAGCACTGC CTTCTCTTCT TGCAGCTGCC TGTGGGGACC CAGATGCTGA GCCTGGGGCC 
AGGATGACTT GTCGGCTTCT CCTGCACCTC TTCAGGACAC CCCAGGTCTG CCTCTTTCCC 
CAGGGAGCAG GAAAATTATT TCCTGGAATT CGTTCTTCTT GTGATCGTCA CCTCTTGGCT 
GCTGCTCACA ACAGCATAGA AGTGGGAGCT GTGTTTGCAG TCTTAAAAGC AATTTTGATG 
CTTGGTGACG CTGAGATCGG CAGCAGCAGT GTCACCCCTG TGAAGAACGA GGAGCTGCCC 
GTGAGGGGCT TGCTGGATGA GATCGCTGAG GATGAAATGT GGGCTTCCTC TCACGCTCTG 
AAATCACGTG GAAAAGCTGT TTCCATAGAA ACCGCCAGTT TGAGCGAGTA TGCTAGATAT 
GTGCTAAGGA CCATTTGCCA GCAGGAATGG GTTGGAGAGC ACTGTTTAAA AGAACCTGAG 
AGGCTGTGCA CAGATAAAGA TCTAATTTTG GATCCCGTTC TTTCAAACAA GCAAGCACAG 
AAACTTCTTC AGCTTATCTG TTATCCTCAT GGTGTGAAAG AATGCTCCGA GGGGGACAAC 
CCTCAGAGGC AGCACATCAA ACGCATACTT CAGAACTTAG ACCAGTGGAC TCTGCGGCAG 
TCTTGGTTGG AGCTGCAACT AATGATCAAG CAGTGCATGA AGGAGCCTGG TTCTGGGTCT 
GTGGCTGAAA TGAACAGCTT GTTGGATAAT ATTGCAAAGG CGACAATAGA GGTGTTTCAG 
CAATCCGCTG ATCTAAACAA TAACTCTTCT AACTCTGGTA TAGGCCTCTT CAATCCAAAC 
TCTGTTGGAA ATGCTGACAC AAGTAATACA AGACAGAATG GTAAAAAGAC ATTCCTAAGT 
TCCTCCGAGT GGCGAGGAGT GTGGCTGGTG GCTCCCTTGA TTGCAAAACT ACCTACCTCA 
GTTCAAGGTA GGGTCTTGAA AGCTGCGGGA GAGGAGCTGG AGAAAGGTCA GCATTTGGGG 
TCATCTTCTA AGAAGGAAAG AGATAGACAG AAGCAAAAGA GCATGTCTCT CTTGAGTCAG 
CAGCCTTTCC TTTCTTTGGT ACTTACTTGC CTTAAGGGAC AGGATGAGCA GCGGGAAGGG 
CTTCTAACAT CACTGCAGAA TCAAGTCAAT CAGATCCTTA GTAACTGGAG GGAAGAACGG 
TACCAGGGTG ATGTTAAAGC TAGGCAAATG ATGCATGAAG CCTTACAGCT TCGTCTGAAT 
TTGGTAGGTG GCATGTTTGA TACTGTGCAG AGGAGTACCC AGTGGACCAC AGACTGGGCA 
CTTCTGCTCC TCCAGATAAT CACTTCGGGA ACAGTTGATA TGCAGACCAA CAACGAATTG 
TTCACAACCG TGCTGGATAT GCTGGGTGTC CTCATCAACG GGACGCTCGC CTCCGACTTG 
TCCAGTGCTT CCCAAGGGGG ACCCGAGGAA AACAAAAGGG CTTACATGAA CTTAGTGAAA 
AAGTTAAAAA AGGAGCTGGG AGACAAGCGG TCAGACAGCA TTGACAAGGT TCGCCAGCTG 
CTCCCTTTGC CAAAGCAGAC ATGTGATATT ATTACCTGTG AGCCAATGGG ATCCTTGATT 
GACACCAAGG GCAACAAAAT TGCGGGGTTT GACTCCATTG ATAAGAAACA GGGTCTTCAG 
GTGTCAACAA AACAAAAGGT GTCCCCTTGG GATTTGTTTG AAGGTCATAA AAATCCTGCT 
CCTCTCTCCT GGGCCTGGTT TGGTACAGTT CGTGTCGACA GGAAGGTGAT CAAATACGAA 
GAGCAGCAGC ATCTGCTCCT GTACCATGCG CACCCCAAAC CCAAGCCGCG GAGCTACTAC 
CTCGAGCCCC TGCCTCTGCC TCCGGAGGAG GAGGAGGAGG AGGAGCCCAC CACCCCTGTC 
TCTCAGGAAC CAGAAAGGAA GTCAGGAGAT CAGGATCAGG GAAAGCATGC CACAGATGAC 
GAGAAGAAGA CAAAGGGCAG GAAGAGGAAG TCAAAGTCAG GCTCTAAGGC TGATGAATAT 
CCACAGAACA ACTTATACAG AGTGCCTCCC AATTACTCTC CCATTCCCTC ACAAATGATG 
CAGCATCCTC AGTCTGCCCT GTGGGGCTAC ACCATCATGG GGCAGCCGCA GCAGCCTGGC 
TTCTTCGTGC AGAACCAGCC CCTCCCTCCA GGTGGTTCCA GGCTGGATCC AACGAGCTCC 
TTCGTCCCAG CCAACACGAA GCAGGCCCTG TCCAACATGC TGCAGCGGCG CTCCGGCACC 
GTGCTGCAGC CCCCTGCCCT CCACGCAGTC ACACCTCAGC AGCAGTTGCT CCAGATGAAA 
CTCCTGCAGC AAGAGCAGCA GCAGCAGCGG CTCCTCAGGC ACCAAGCACA GGCACGGTCT 
CTCCAGCAGA GCATGCCCCA CGGGTACACG ATGTACAGCA CCCCGATGCC TTTGCAGCAG 
CAGCAGCAGC CCAGCAGCGT GGTGCTCTCT CCTGGCTACA CCCCGCGGGC ATACACAGCC 
CACTCCAGCC CCGTGCTGAT GGAGCGGCTG CGGCCGGGGC AGCCGGGCAG CGTGGCTCAG 
CAGCAGGCAG CTGCCTACAT GCAGCCCCTG GCGGGTGCTC AGCGGTTGAC CCATCAGCCT 
CTGCAGCCAA ACTCATTGAT AGGGGCAGGC CTGGACAGTG CATCAACCGC ACGTACACAT 
CCAACCTTGA GCTCAGCACA GCTGCCCCAG GAGCAAATGC GACAGAGGCA GCAGGAGATC 
CGACAGCAGA GACTCTTCCA GATGCAGCAG GGGCAGCAGC AGCAGACTCT TGATCTTCAG 
CCTGTGCAGC CACAGCAGCC CCTGGTAAGT GGGATGGATG GTGAGCGTGG GGCAGGGGCG 
TGGGATGAGC GGGTGAAGCT TACAGTGAAT TTGCAGTGCT GTTGTCCATG TTTCCTGGAC 
ATGGCCATTT TTTCCAGATA TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_015291835.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147321.1 Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X8, mRNA.

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CloneID	OGa40174
Clone ID Related Accession (Same CDS sequence)	XM_015291835.1
Accession Version	XM_015291835.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGAT TTTTTCTCCG 
ATGCCTGGGG AGTCCTGTGA GAACATGAAC TCTTCTTTAG ACAGAAGAAT TTCTGTTGCC 
AGTGAGAAGT CAGTCAAGAG GGAAAGACTC AGAGAAGTGA TTTTTCCATC TAACTATGAC 
CTCCTTCGTC ATCTGCAATA TGCAACGCAT TTCCCAATAC CTCTGGATGA ATCCTCAAGC 
CATGAGTGCA ACCAGCGCAT GATTCTTCTC TATGGTGTTG GCAAGGAGCG TGATGAAGCA 
AGACATCAGC TGAAGAAGAT TACCAAAGAC ATCCTGAAGA TTCTGAATAA GAAGAGTACT 
ACTGAAACGG GTGTTGGGGA TGAAGGGCAA AAAGCCAGGA AGAACAAACA AGAAGCATTT 
CCAACTCTAG AGACTGTGTT CACAAAACTA CAGCAGCTGT CATATTTTGA CCAACATCAG 
GTGACATCTC AGATCTCCAG CAATGTTCTC GAACAAATAA CCAGCTTTGC CTCAGGAACA 
TCCTATCACC TCCCCTTGGC TCACCACATA CAGCTCATCT TTGATCTCAT GGAACCAGCA 
CTGAATATCA ACGGGCTGAT AGACTTTGCA ATACAGCTGC TGAATGAGCT GAGCGTGGTG 
GAGGCAGAAC TGCTGTTGAA ATCCTCCGAC CTTGTTGGCA GCTACACCAC CAGCTTGTGC 
CTGTGCATCG TTGCCGTCCT GCGGCGTTAC CACGCCTGCC TCATTCTCAA CTCGGAGCAA 
ACTGCACAAG TCTTTGAAGG GTTGTGTGGA GTGGTGAAGC ACGTTGTTAA TCCCTCAGAG 
TGTTCCTCCC CAGAAAGATG TATTTTGGCC TACCTCTATG ACCTGTATGT GTCCTGCAGC 
CACCTGCGAA GCAAGTTTGG AGACCTCTTC AGTAGTGCCT GTTCTAAGGT GAAGCAAACG 
ATATACAGCA ATGTTCAGCC TTCAAATTCC AATCTGCTGT GGGACCCGGA GTTCATGCTT 
GATTTTATTG AGAACCCTTC TGCTCACAGC ATTAACTACT CCATGCTGGG CAAGATCCTG 
AGTGACAACG CAGCCAACCG CTACAGCTTT GTCTGCAATG CTCTAATGAA TGTGTGCATG 
GGGCACCAAG ATGCAGGGAG GATTAATGAC ATAGCAAACC TTTGTGCAGA GCTGACGGCG 
TGCTGCACCG TGCTGAGCTC GGAGTGGCTG GGGGTTGTCA AAGCTCTTTG CTGCTCTTCC 
AATCACGTGT GGGGTTTCAA TGATCTGCTC TGCAGCGTGG ATGTGAGTGA CCTGTCATTC 
CACGATTCCC TGGCCACTTT CATTGCCATA CTGATTGCCC GGCAGTGCTT TTCTCTGGAA 
GATGTCGTTC AGCACGTAGC ACTGCCTTCT CTTCTTGCAG CTGCCTGTGG GGACCCAGAT 
GCTGAGCCTG GGGCCAGGAT GACTTGTCGG CTTCTCCTGC ACCTCTTCAG GACACCCCAG 
GTCTGCCTCT TTCCCCAGGG AGCAGGAAAA TTATTTCCTG GAATTCGTTC TTCTTGTGAT 
CGTCACCTCT TGGCTGCTGC TCACAACAGC ATAGAAGTGG GAGCTGTGTT TGCAGTCTTA 
AAAGCAATTT TGATGCTTGG TGACGCTGAG ATCGGCAGCA GCAGTGTCAC CCCTGTGAAG 
AACGAGGAGC TGCCCGTGAG GGGCTTGCTG GATGAGATCG CTGAGGATGA AATGTGGGCT 
TCCTCTCACG CTCTGAAATC ACGTGGAAAA GCTGTTTCCA TAGAAACCGC CAGTTTGAGC 
GAGTATGCTA GATATGTGCT AAGGACCATT TGCCAGCAGG AATGGGTTGG AGAGCACTGT 
TTAAAAGAAC CTGAGAGGCT GTGCACAGAT AAAGATCTAA TTTTGGATCC CGTTCTTTCA 
AACAAGCAAG CACAGAAACT TCTTCAGCTT ATCTGTTATC CTCATGGTGT GAAAGAATGC 
TCCGAGGGGG ACAACCCTCA GAGGCAGCAC ATCAAACGCA TACTTCAGAA CTTAGACCAG 
TGGACTCTGC GGCAGTCTTG GTTGGAGCTG CAACTAATGA TCAAGCAGTG CATGAAGGAG 
CCTGGTTCTG GGTCTGTGGC TGAAATGAAC AGCTTGTTGG ATAATATTGC AAAGGCGACA 
ATAGAGGTGT TTCAGCAATC CGCTGATCTA AACAATAACT CTTCTAACTC TGGTATAGGC 
CTCTTCAATC CAAACTCTGT TGGAAATGCT GACACAAGTA ATACAAGACA GAATGGTAAA 
AAGACATTCC TAAGTTCCTC CGAGTGGCGA GGAGTGTGGC TGGTGGCTCC CTTGATTGCA 
AAACTACCTA CCTCAGTTCA AGGTAGGGTC TTGAAAGCTG CGGGAGAGGA GCTGGAGAAA 
GGTCAGCATT TGGGGTCATC TTCTAAGAAG GAAAGAGATA GACAGAAGCA AAAGAGCATG 
TCTCTCTTGA GTCAGCAGCC TTTCCTTTCT TTGGTACTTA CTTGCCTTAA GGGACAGGAT 
GAGCAGCGGG AAGGGCTTCT AACATCACTG CAGAATCAAG TCAATCAGAT CCTTAGTAAC 
TGGAGGGAAG AACGGTACCA GGGTGATGTT AAAGCTAGGC AAATGATGCA TGAAGCCTTA 
CAGCTTCGTC TGAATTTGGT AGGTGGCATG TTTGATACTG TGCAGAGGAG TACCCAGTGG 
ACCACAGACT GGGCACTTCT GCTCCTCCAG ATAATCACTT CGGGAACAGT TGATATGCAG 
ACCAACAACG AATTGTTCAC AACCGTGCTG GATATGCTGG GTGTCCTCAT CAACGGGACG 
CTCGCCTCCG ACTTGTCCAG TGCTTCCCAA GGGGGACCCG AGGAAAACAA AAGGGCTTAC 
ATGAACTTAG TGAAAAAGTT AAAAAAGGAG CTGGGAGACA AGCGGTCAGA CAGCATTGAC 
AAGGTTCGCC AGCTGCTCCC TTTGCCAAAG CAGACATGTG ATATTATTAC CTGTGAGCCA 
ATGGGATCCT TGATTGACAC CAAGGGCAAC AAAATTGCGG GGTTTGACTC CATTGATAAG 
AAACAGGGTC TTCAGGTGTC AACAAAACAA AAGGTGTCCC CTTGGGATTT GTTTGAAGGT 
CATAAAAATC CTGCTCCTCT CTCCTGGGCC TGGTTTGGTA CAGTTCGTGT CGACAGGAAG 
GTGATCAAAT ACGAAGAGCA GCAGCATCTG CTCCTGTACC ATGCGCACCC CAAACCCAAG 
CCGCGGAGCT ACTACCTCGA GCCCCTGCCT CTGCCTCCGG AGGAGGAGGA GGAGGAGGAG 
CCCACCACCC CTGTCTCTCA GGAACCAGAA AGGAAGTCAG GAGATCAGGA TCAGGGAAAG 
CATGCCACAG ATGACGAGAA GAAGACAAAG GGCAGGAAGA GGAAGTCAAA GTCAGGCTCT 
AAGGCTGATG AATATCCACA GAACAACTTA TACAGAGTGC CTCCCAATTA CTCTCCCATT 
CCCTCACAAA TGATGCAGCA TCCTCAGTCT GCCCTGTGGG GCTACACCAT CATGGGGCAG 
CCGCAGCAGC CTGGCTTCTT CGTGCAGAAC CAGCCCCTCC CTCCAGGTGG TTCCAGGCTG 
GATCCAACGA GCTCCTTCGT CCCAGCCAAC ACGAAGCAGG CCCTGTCCAA CATGCTGCAG 
CGGCGCTCCG GCACCGTGCT GCAGCCCCCT GCCCTCCACG CAGTCACACC TCAGCAGCAG 
TTGCTCCAGA TGAAACTCCT GCAGCAAGAG CAGCAGCAGC AGCGGCTCCT CAGGCACCAA 
GCACAGGCAC GGTCTCTCCA GCAGGGTCAG CCGATGGACC CCGCTGCTCT TTTCACCCCG 
CAAGTCCGAC CCACGCCACA GCTACCGCAG TACCCAGGGC TTCAGCAAGC ACAGAGCATG 
CCCCACGGGT ACACGATGTA CAGCACCCCG ATGCCTTTGC AGCAGCAGCA GCAGCCCAGC 
AGCGTGGTGC TCTCTCCTGG CTACACCCCG CGGGCATACA CAGCCCACTC CAGCCCCGTG 
CTGATGGAGC GGCTGCGGCC GGGGCAGCCG GGCAGCGTGG CTCAGCAGCA GGCAGCTGCC 
TACATGCAGC CCCTGGCGGG TGCTCAGCGG TTGACCCATC AGCCTCTGCA GCCAAACTCA 
TTGATAGGGG CAGGCCTGGA CAGTGCATCA ACCGCACGTA CACATCCAAC CTTGAGCTCA 
GCACAGCTGC CCCAGGAGCA AATGCGACAG AGGCAGCAGG AGATCCGACA GCAGAGACTC 
TTCCAGATGC AGCAGGGGCA GCAGCAGCAG ACTCTTGATC TTCAGCCTGT GCAGCCACAG 
CAGCCCCTGG TAAGTGGGAT GGATGGTGAG CGTGGGGCAG GGGCGTGGGA TGAGCGGGTG 
AAGCTTACAG TGAATTTGCA GTGCTGTTGT CCATGTTTCC TGGACATGGC CATTTTTTCC 
AGATATGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147321.1
CDS	1..6372
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPRQKEDELTAVNVKQGFSNQPAFSGDEHGSARNIVINASKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHNWFTDLAGSKPLTILAKKVPILSKKEDVFAYLAKYSVPLLRAAWLIKMTCAYYAAISEAKIKKRQATDPNIEWTQIITRYLREQLAKVAEFYHVTSSQGNSSVVMPQEMEQALKQWEYNEKLSFYMFQEGMLERHEYLTWILDVLEKIRPTDDDILKLVLPLMLQYSEEFVQSAYLSRRLAYFCARRLSLLLSDGPSLAAAHSPHVIIAPSNPPLAVPSPTAPGPVLSPVQLTCSDFLSCPQHRPLVYGLSCMLQTITLCCPSALVWNYSTNENKNMNPGSPLDLLQVAPSSLPMPGGNSTFNQQVRAKIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRSDSSNSMETLYHKIFWANQNKDNQEAAPSDEAVVMLLCEWAVSCKRSGKHRAMVVAKLLEKRQAEIEAERCGEAEVLDEKESLSSASLTSSSLPVFQNILLRFLDTQAPSLSDPNSDHEKTEFVNLVLLFSEFTRHDVFSHDAYMCTLISRGDLSITATTRPRSPNGETGDEHYSKDHDMKMEIFSPMPGESCENMNSSLDRRISVASEKSVKRERLREVIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRMILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQEAFPTLETVFTKLQQLSYFDQHQVTSQISSNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRRYHACLILNSEQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYSNVQPSNSNLLWDPEFMLDFIENPSAHSINYSMLGKILSDNAANRYSFVCNALMNVCMGHQDAGRINDIANLCAELTACCTVLSSEWLGVVKALCCSSNHVWGFNDLLCSVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDPDAEPGARMTCRLLLHLFRTPQVCLFPQGAGKLFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAILMLGDAEIGSSSVTPVKNEELPVRGLLDEIAEDEMWASSHALKSRGKAVSIETASLSEYARYVLRTICQQEWVGEHCLKEPERLCTDKDLILDPVLSNKQAQKLLQLICYPHGVKECSEGDNPQRQHIKRILQNLDQWTLRQSWLELQLMIKQCMKEPGSGSVAEMNSLLDNIAKATIEVFQQSADLNNNSSNSGIGLFNPNSVGNADTSNTRQNGKKTFLSSSEWRGVWLVAPLIAKLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQGDVKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMQTNNELFTTVLDMLGVLINGTLASDLSSASQGGPEENKRAYMNLVKKLKKELGDKRSDSIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGHKNPAPLSWAWFGTVRVDRKVIKYEEQQHLLLYHAHPKPKPRSYYLEPLPLPPEEEEEEEPTTPVSQEPERKSGDQDQGKHATDDEKKTKGRKRKSKSGSKADEYPQNNLYRVPPNYSPIPSQMMQHPQSALWGYTIMGQPQQPGFFVQNQPLPPGGSRLDPTSSFVPANTKQALSNMLQRRSGTVLQPPALHAVTPQQQLLQMKLLQQEQQQQRLLRHQAQARSLQQGQPMDPAALFTPQVRPTPQLPQYPGLQQAQSMPHGYTMYSTPMPLQQQQQPSSVVLSPGYTPRAYTAHSSPVLMERLRPGQPGSVAQQQAAAYMQPLAGAQRLTHQPLQPNSLIGAGLDSASTARTHPTLSSAQLPQEQMRQRQQEIRQQRLFQMQQGQQQQTLDLQPVQPQQPLVSGMDGERGAGAWDERVKLTVNLQCCCPCFLDMAIFSRYV

Target ORF information:

RefSeq Version	XM_015291835.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291835.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTCCT CAGCTATGAG CAGAGACCGC TCAAGCGGCC GCGCCTGGGG 
CCGCCCGACG TCTACCCGCA GGACCCGCGC CAGAAGGAGG ACGAACTTAC TGCGGTGAAT 
GTAAAGCAAG GCTTCAGTAA TCAGCCTGCC TTCTCTGGAG ATGAGCATGG TTCTGCTAGA 
AATATTGTCA TCAACGCCTC TAAGATTGGC GCTTACTTCA GCAGCATATT AGCAGAAAAG 
CTAAAACTTA ATACGTTCCA GGACACGGGA AAGAAGAAAC CACAAGTAAA TGCCAAAGAC 
AACTACTGGC TGGTTACTGC TCGCTCTCAG AGTGCAATTC ACAACTGGTT CACAGATCTA 
GCAGGAAGTA AACCACTGAC TATTTTAGCA AAAAAGGTTC CAATTCTCAG TAAAAAAGAA 
GATGTTTTTG CTTACTTAGC AAAATATTCT GTACCACTGC TGCGAGCAGC ATGGCTGATC 
AAAATGACAT GTGCCTACTA TGCTGCCATT TCTGAAGCTA AAATCAAGAA ACGCCAGGCC 
ACTGACCCAA ATATTGAATG GACTCAAATT ATAACCAGAT ACCTTCGAGA GCAGCTGGCA 
AAGGTTGCAG AGTTTTATCA TGTGACTTCC AGCCAAGGCA ACAGCTCTGT AGTGATGCCT 
CAAGAGATGG AACAGGCCTT GAAGCAGTGG GAATATAATG AAAAATTGTC CTTTTATATG 
TTTCAGGAAG GAATGCTTGA GAGACACGAG TACTTGACAT GGATACTGGA TGTTCTGGAA 
AAAATCAGAC CTACTGATGA CGACATTCTT AAGCTGGTGC TGCCACTAAT GTTGCAGTAT 
TCAGAAGAGT TTGTTCAGTC AGCTTACCTG TCCCGCCGCC TTGCTTATTT CTGTGCCAGA 
CGCCTGTCGT TACTGCTGAG CGATGGCCCC AGCCTTGCAG CTGCACATTC TCCTCATGTG 
ATTATTGCAC CAAGTAACCC TCCTTTGGCA GTGCCGAGTC CGACTGCACC CGGTCCTGTG 
CTGAGCCCTG TGCAGCTCAC CTGCTCAGAT TTCCTCTCCT GCCCACAACA CCGCCCGCTG 
GTGTATGGGC TGAGTTGTAT GCTGCAGACA ATAACTCTTT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAAC ATGAATCCTG GCTCACCCCT GGATTTGCTT 
CAAGTTGCTC CATCTAGTTT ACCTATGCCA GGTGGAAATT CAACATTTAA TCAGCAGGTT 
CGGGCAAAGA TCTATGAGGT AGAACAGCAG ATAAAACAAA GAGGTCGTGC TGTGGAGGTG 
CGGTGGTCTT TTGACAAGTG CCAAGAATCA ACTGCAGGGG TGACCATCAG CCGAGTTCTG 
CATACCTTAG AAGTGTTGGA TCGACATTGT TTTGACAGAT CAGATTCCAG CAACTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAGAATA AAGATAACCA AGAGGCAGCC 
CCCAGCGATG AGGCCGTGGT GATGCTGCTG TGTGAGTGGG CTGTGAGCTG CAAGCGGTCT 
GGGAAGCACC GGGCCATGGT GGTGGCCAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAA 
GCAGAAAGAT GTGGTGAAGC TGAAGTCCTA GATGAAAAGG AATCTCTGTC TTCAGCCTCC 
TTGACAAGTT CCAGTCTTCC TGTTTTCCAG AACATCCTGC TGAGGTTTTT GGACACACAG 
GCTCCCTCGC TGTCTGACCC AAATAGCGAC CATGAGAAGA CGGAGTTTGT GAATTTGGTG 
CTGCTGTTTT CTGAATTCAC TCGACACGAT GTTTTCTCCC ATGATGCATA CATGTGCACT 
TTAATATCAC GTGGAGACTT GTCAATCACT GCAACTACGA GACCACGTTC ACCTAATGGG 
GAAACTGGGG ATGAACACTA TTCTAAGGAT CATGATATGA AAATGGAGAT TTTTTCTCCG 
ATGCCTGGGG AGTCCTGTGA GAACATGAAC TCTTCTTTAG ACAGAAGAAT TTCTGTTGCC 
AGTGAGAAGT CAGTCAAGAG GGAAAGACTC AGAGAAGTGA TTTTTCCATC TAACTATGAC 
CTCCTTCGTC ATCTGCAATA TGCAACGCAT TTCCCAATAC CTCTGGATGA ATCCTCAAGC 
CATGAGTGCA ACCAGCGCAT GATTCTTCTC TATGGTGTTG GCAAGGAGCG TGATGAAGCA 
AGACATCAGC TGAAGAAGAT TACCAAAGAC ATCCTGAAGA TTCTGAATAA GAAGAGTACT 
ACTGAAACGG GTGTTGGGGA TGAAGGGCAA AAAGCCAGGA AGAACAAACA AGAAGCATTT 
CCAACTCTAG AGACTGTGTT CACAAAACTA CAGCAGCTGT CATATTTTGA CCAACATCAG 
GTGACATCTC AGATCTCCAG CAATGTTCTC GAACAAATAA CCAGCTTTGC CTCAGGAACA 
TCCTATCACC TCCCCTTGGC TCACCACATA CAGCTCATCT TTGATCTCAT GGAACCAGCA 
CTGAATATCA ACGGGCTGAT AGACTTTGCA ATACAGCTGC TGAATGAGCT GAGCGTGGTG 
GAGGCAGAAC TGCTGTTGAA ATCCTCCGAC CTTGTTGGCA GCTACACCAC CAGCTTGTGC 
CTGTGCATCG TTGCCGTCCT GCGGCGTTAC CACGCCTGCC TCATTCTCAA CTCGGAGCAA 
ACTGCACAAG TCTTTGAAGG GTTGTGTGGA GTGGTGAAGC ACGTTGTTAA TCCCTCAGAG 
TGTTCCTCCC CAGAAAGATG TATTTTGGCC TACCTCTATG ACCTGTATGT GTCCTGCAGC 
CACCTGCGAA GCAAGTTTGG AGACCTCTTC AGTAGTGCCT GTTCTAAGGT GAAGCAAACG 
ATATACAGCA ATGTTCAGCC TTCAAATTCC AATCTGCTGT GGGACCCGGA GTTCATGCTT 
GATTTTATTG AGAACCCTTC TGCTCACAGC ATTAACTACT CCATGCTGGG CAAGATCCTG 
AGTGACAACG CAGCCAACCG CTACAGCTTT GTCTGCAATG CTCTAATGAA TGTGTGCATG 
GGGCACCAAG ATGCAGGGAG GATTAATGAC ATAGCAAACC TTTGTGCAGA GCTGACGGCG 
TGCTGCACCG TGCTGAGCTC GGAGTGGCTG GGGGTTGTCA AAGCTCTTTG CTGCTCTTCC 
AATCACGTGT GGGGTTTCAA TGATCTGCTC TGCAGCGTGG ATGTGAGTGA CCTGTCATTC 
CACGATTCCC TGGCCACTTT CATTGCCATA CTGATTGCCC GGCAGTGCTT TTCTCTGGAA 
GATGTCGTTC AGCACGTAGC ACTGCCTTCT CTTCTTGCAG CTGCCTGTGG GGACCCAGAT 
GCTGAGCCTG GGGCCAGGAT GACTTGTCGG CTTCTCCTGC ACCTCTTCAG GACACCCCAG 
GTCTGCCTCT TTCCCCAGGG AGCAGGAAAA TTATTTCCTG GAATTCGTTC TTCTTGTGAT 
CGTCACCTCT TGGCTGCTGC TCACAACAGC ATAGAAGTGG GAGCTGTGTT TGCAGTCTTA 
AAAGCAATTT TGATGCTTGG TGACGCTGAG ATCGGCAGCA GCAGTGTCAC CCCTGTGAAG 
AACGAGGAGC TGCCCGTGAG GGGCTTGCTG GATGAGATCG CTGAGGATGA AATGTGGGCT 
TCCTCTCACG CTCTGAAATC ACGTGGAAAA GCTGTTTCCA TAGAAACCGC CAGTTTGAGC 
GAGTATGCTA GATATGTGCT AAGGACCATT TGCCAGCAGG AATGGGTTGG AGAGCACTGT 
TTAAAAGAAC CTGAGAGGCT GTGCACAGAT AAAGATCTAA TTTTGGATCC CGTTCTTTCA 
AACAAGCAAG CACAGAAACT TCTTCAGCTT ATCTGTTATC CTCATGGTGT GAAAGAATGC 
TCCGAGGGGG ACAACCCTCA GAGGCAGCAC ATCAAACGCA TACTTCAGAA CTTAGACCAG 
TGGACTCTGC GGCAGTCTTG GTTGGAGCTG CAACTAATGA TCAAGCAGTG CATGAAGGAG 
CCTGGTTCTG GGTCTGTGGC TGAAATGAAC AGCTTGTTGG ATAATATTGC AAAGGCGACA 
ATAGAGGTGT TTCAGCAATC CGCTGATCTA AACAATAACT CTTCTAACTC TGGTATAGGC 
CTCTTCAATC CAAACTCTGT TGGAAATGCT GACACAAGTA ATACAAGACA GAATGGTAAA 
AAGACATTCC TAAGTTCCTC CGAGTGGCGA GGAGTGTGGC TGGTGGCTCC CTTGATTGCA 
AAACTACCTA CCTCAGTTCA AGGTAGGGTC TTGAAAGCTG CGGGAGAGGA GCTGGAGAAA 
GGTCAGCATT TGGGGTCATC TTCTAAGAAG GAAAGAGATA GACAGAAGCA AAAGAGCATG 
TCTCTCTTGA GTCAGCAGCC TTTCCTTTCT TTGGTACTTA CTTGCCTTAA GGGACAGGAT 
GAGCAGCGGG AAGGGCTTCT AACATCACTG CAGAATCAAG TCAATCAGAT CCTTAGTAAC 
TGGAGGGAAG AACGGTACCA GGGTGATGTT AAAGCTAGGC AAATGATGCA TGAAGCCTTA 
CAGCTTCGTC TGAATTTGGT AGGTGGCATG TTTGATACTG TGCAGAGGAG TACCCAGTGG 
ACCACAGACT GGGCACTTCT GCTCCTCCAG ATAATCACTT CGGGAACAGT TGATATGCAG 
ACCAACAACG AATTGTTCAC AACCGTGCTG GATATGCTGG GTGTCCTCAT CAACGGGACG 
CTCGCCTCCG ACTTGTCCAG TGCTTCCCAA GGGGGACCCG AGGAAAACAA AAGGGCTTAC 
ATGAACTTAG TGAAAAAGTT AAAAAAGGAG CTGGGAGACA AGCGGTCAGA CAGCATTGAC 
AAGGTTCGCC AGCTGCTCCC TTTGCCAAAG CAGACATGTG ATATTATTAC CTGTGAGCCA 
ATGGGATCCT TGATTGACAC CAAGGGCAAC AAAATTGCGG GGTTTGACTC CATTGATAAG 
AAACAGGGTC TTCAGGTGTC AACAAAACAA AAGGTGTCCC CTTGGGATTT GTTTGAAGGT 
CATAAAAATC CTGCTCCTCT CTCCTGGGCC TGGTTTGGTA CAGTTCGTGT CGACAGGAAG 
GTGATCAAAT ACGAAGAGCA GCAGCATCTG CTCCTGTACC ATGCGCACCC CAAACCCAAG 
CCGCGGAGCT ACTACCTCGA GCCCCTGCCT CTGCCTCCGG AGGAGGAGGA GGAGGAGGAG 
CCCACCACCC CTGTCTCTCA GGAACCAGAA AGGAAGTCAG GAGATCAGGA TCAGGGAAAG 
CATGCCACAG ATGACGAGAA GAAGACAAAG GGCAGGAAGA GGAAGTCAAA GTCAGGCTCT 
AAGGCTGATG AATATCCACA GAACAACTTA TACAGAGTGC CTCCCAATTA CTCTCCCATT 
CCCTCACAAA TGATGCAGCA TCCTCAGTCT GCCCTGTGGG GCTACACCAT CATGGGGCAG 
CCGCAGCAGC CTGGCTTCTT CGTGCAGAAC CAGCCCCTCC CTCCAGGTGG TTCCAGGCTG 
GATCCAACGA GCTCCTTCGT CCCAGCCAAC ACGAAGCAGG CCCTGTCCAA CATGCTGCAG 
CGGCGCTCCG GCACCGTGCT GCAGCCCCCT GCCCTCCACG CAGTCACACC TCAGCAGCAG 
TTGCTCCAGA TGAAACTCCT GCAGCAAGAG CAGCAGCAGC AGCGGCTCCT CAGGCACCAA 
GCACAGGCAC GGTCTCTCCA GCAGGGTCAG CCGATGGACC CCGCTGCTCT TTTCACCCCG 
CAAGTCCGAC CCACGCCACA GCTACCGCAG TACCCAGGGC TTCAGCAAGC ACAGAGCATG 
CCCCACGGGT ACACGATGTA CAGCACCCCG ATGCCTTTGC AGCAGCAGCA GCAGCCCAGC 
AGCGTGGTGC TCTCTCCTGG CTACACCCCG CGGGCATACA CAGCCCACTC CAGCCCCGTG 
CTGATGGAGC GGCTGCGGCC GGGGCAGCCG GGCAGCGTGG CTCAGCAGCA GGCAGCTGCC 
TACATGCAGC CCCTGGCGGG TGCTCAGCGG TTGACCCATC AGCCTCTGCA GCCAAACTCA 
TTGATAGGGG CAGGCCTGGA CAGTGCATCA ACCGCACGTA CACATCCAAC CTTGAGCTCA 
GCACAGCTGC CCCAGGAGCA AATGCGACAG AGGCAGCAGG AGATCCGACA GCAGAGACTC 
TTCCAGATGC AGCAGGGGCA GCAGCAGCAG ACTCTTGATC TTCAGCCTGT GCAGCCACAG 
CAGCCCCTGG TAAGTGGGAT GGATGGTGAG CGTGGGGCAG GGGCGTGGGA TGAGCGGGTG 
AAGCTTACAG TGAATTTGCA GTGCTGTTGT CCATGTTTCC TGGACATGGC CATTTTTTCC 
AGATATGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_015291837.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147323.1 Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X10, mRNA.

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CloneID	OGa40176
Clone ID Related Accession (Same CDS sequence)	XM_015291837.1
Accession Version	XM_015291837.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5859bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCCAT TTCTGAAGCT AAAATCAAGA AACGCCAGGC CACTGACCCA AATATTGGAA 
GGAATGCTTG AGAGACACGA GTACTTGACA TGGATACTGG ATGTTCTGGA AAAAATCAGA 
CCTACTGATG ACGACATTCT TAAGCTGGTG CTGCCACTAA TGTTGCAGTA TTCAGAAGAG 
TTTGTTCAGT CAGCTTACCT GTCCCGCCGC CTTGCTTATT TCTGTGCCAG ACGCCTGTCG 
TTACTGCTGA GCGATGGCCC CAGCCTTGCA GCTGCACATT CTCCTCATGT GATTATTGCA 
CCAAGTAACC CTCCTTTGGC AGTGCCGAGT CCGACTGCAC CCGGTCCTGT GCTGAGCCCT 
GTGCAGCTCA CCTGCTCAGA TTTCCTCTCC TGCCCACAAC ACCGCCCGCT GGTGTATGGG 
CTGAGTTGTA TGCTGCAGAC AATAACTCTT TGTTGCCCAA GTGCCTTGGT GTGGAATTAT 
TCCACAAATG AAAATAAGAA CATGAATCCT GGCTCACCCC TGGATTTGCT TCAAGTTGCT 
CCATCTAGTT TACCTATGCC AGGTGGAAAT TCAACATTTA ATCAGCAGGT TCGGGCAAAG 
ATCTATGAGG TAGAACAGCA GATAAAACAA AGAGGTCGTG CTGTGGAGGT GCGGTGGTCT 
TTTGACAAGT GCCAAGAATC AACTGCAGGG GTGACCATCA GCCGAGTTCT GCATACCTTA 
GAAGTGTTGG ATCGACATTG TTTTGACAGA TCAGATTCCA GCAACTCAAT GGAGACGCTT 
TATCATAAGA TTTTCTGGGC AAACCAGAAT AAAGATAACC AAGAGGCAGC CCCCAGCGAT 
GAGGCCGTGG TGATGCTGCT GTGTGAGTGG GCTGTGAGCT GCAAGCGGTC TGGGAAGCAC 
CGGGCCATGG TGGTGGCCAA ACTCCTGGAG AAGAGGCAAG CAGAAATTGA AGCAGAAAGA 
TGTGGTGAAG CTGAAGTCCT AGATGAAAAG GAATCTCTGT CTTCAGCCTC CTTGACAAGT 
TCCAGTCTTC CTGTTTTCCA GAACATCCTG CTGAGGTTTT TGGACACACA GGCTCCCTCG 
CTGTCTGACC CAAATAGCGA CCATGAGAAG ACGGAGTTTG TGAATTTGGT GCTGCTGTTT 
TCTGAATTCA CTCGACACGA TGTTTTCTCC CATGATGCAT ACATGTGCAC TTTAATATCA 
CGTGGAGACT TGTCAATCAC TGCAACTACG AGACCACGTT CACCTAATGG GGAAACTGGG 
GATGAACACT ATTCTAAGGA TCATGATATG AAAATGGAGG ATCATAATAT TATGGAGCAC 
ATGGGCACTG ACTCAGGACC CACTAGTATC TTTGATGATG TAGACAAGGG TGACTTTAAG 
GCAGATTTTG GTTCGGAATT TCCAATTTTT TCTCCGATGC CTGGGGAGTC CTGTGAGAAC 
ATGAACTCTT CTTTAGACAG AAGAATTTCT GTTGCCAGTG AGAAGTCAGT CAAGAGGGAA 
AGACTCAGAG AAGTGATTTT TCCATCTAAC TATGACCTCC TTCGTCATCT GCAATATGCA 
ACGCATTTCC CAATACCTCT GGATGAATCC TCAAGCCATG AGTGCAACCA GCGCATGATT 
CTTCTCTATG GTGTTGGCAA GGAGCGTGAT GAAGCAAGAC ATCAGCTGAA GAAGATTACC 
AAAGACATCC TGAAGATTCT GAATAAGAAG AGTACTACTG AAACGGGTGT TGGGGATGAA 
GGGCAAAAAG CCAGGAAGAA CAAACAAGAA GCATTTCCAA CTCTAGAGAC TGTGTTCACA 
AAACTACAGC AGCTGTCATA TTTTGACCAA CATCAGGTGA CATCTCAGAT CTCCAGCAAT 
GTTCTCGAAC AAATAACCAG CTTTGCCTCA GGAACATCCT ATCACCTCCC CTTGGCTCAC 
CACATACAGC TCATCTTTGA TCTCATGGAA CCAGCACTGA ATATCAACGG GCTGATAGAC 
TTTGCAATAC AGCTGCTGAA TGAGCTGAGC GTGGTGGAGG CAGAACTGCT GTTGAAATCC 
TCCGACCTTG TTGGCAGCTA CACCACCAGC TTGTGCCTGT GCATCGTTGC CGTCCTGCGG 
CGTTACCACG CCTGCCTCAT TCTCAACTCG GAGCAAACTG CACAAGTCTT TGAAGGGTTG 
TGTGGAGTGG TGAAGCACGT TGTTAATCCC TCAGAGTGTT CCTCCCCAGA AAGATGTATT 
TTGGCCTACC TCTATGACCT GTATGTGTCC TGCAGCCACC TGCGAAGCAA GTTTGGAGAC 
CTCTTCAGTA GTGCCTGTTC TAAGGTGAAG CAAACGATAT ACAGCAATGT TCAGCCTTCA 
AATTCCAATC TGCTGTGGGA CCCGGAGTTC ATGCTTGATT TTATTGAGAA CCCTTCTGCT 
CACAGCATTA ACTACTCCAT GCTGGGCAAG ATCCTGAGTG ACAACGCAGC CAACCGCTAC 
AGCTTTGTCT GCAATGCTCT AATGAATGTG TGCATGGGGC ACCAAGATGC AGGGAGGATT 
AATGACATAG CAAACCTTTG TGCAGAGCTG ACGGCGTGCT GCACCGTGCT GAGCTCGGAG 
TGGCTGGGGG TTGTCAAAGC TCTTTGCTGC TCTTCCAATC ACGTGTGGGG TTTCAATGAT 
CTGCTCTGCA GCGTGGATGT GAGTGACCTG TCATTCCACG ATTCCCTGGC CACTTTCATT 
GCCATACTGA TTGCCCGGCA GTGCTTTTCT CTGGAAGATG TCGTTCAGCA CGTAGCACTG 
CCTTCTCTTC TTGCAGCTGC CTGTGGGGAC CCAGATGCTG AGCCTGGGGC CAGGATGACT 
TGTCGGCTTC TCCTGCACCT CTTCAGGACA CCCCAGGTCT GCCTCTTTCC CCAGGGAGCA 
GGAAAATTAT TTCCTGGAAT TCGTTCTTCT TGTGATCGTC ACCTCTTGGC TGCTGCTCAC 
AACAGCATAG AAGTGGGAGC TGTGTTTGCA GTCTTAAAAG CAATTTTGAT GCTTGGTGAC 
GCTGAGATCG GCAGCAGCAG TGTCACCCCT GTGAAGAACG AGGAGCTGCC CGTGAGGGGC 
TTGCTGGATG AGATCGCTGA GGATGAAATG TGGGCTTCCT CTCACGCTCT GAAATCACGT 
GGAAAAGCTG TTTCCATAGA AACCGCCAGT TTGAGCGAGT ATGCTAGATA TGTGCTAAGG 
ACCATTTGCC AGCAGGAATG GGTTGGAGAG CACTGTTTAA AAGAACCTGA GAGGCTGTGC 
ACAGATAAAG ATCTAATTTT GGATCCCGTT CTTTCAAACA AGCAAGCACA GAAACTTCTT 
CAGCTTATCT GTTATCCTCA TGGTGTGAAA GAATGCTCCG AGGGGGACAA CCCTCAGAGG 
CAGCACATCA AACGCATACT TCAGAACTTA GACCAGTGGA CTCTGCGGCA GTCTTGGTTG 
GAGCTGCAAC TAATGATCAA GCAGTGCATG AAGGAGCCTG GTTCTGGGTC TGTGGCTGAA 
ATGAACAGCT TGTTGGATAA TATTGCAAAG GCGACAATAG AGGTGTTTCA GCAATCCGCT 
GATCTAAACA ATAACTCTTC TAACTCTGGT ATAGGCCTCT TCAATCCAAA CTCTGTTGGA 
AATGCTGACA CAAGTAATAC AAGACAGAAT GGTAAAAAGA CATTCCTAAG TTCCTCCGAG 
TGGCGAGGAG TGTGGCTGGT GGCTCCCTTG ATTGCAAAAC TACCTACCTC AGTTCAAGGT 
AGGGTCTTGA AAGCTGCGGG AGAGGAGCTG GAGAAAGGTC AGCATTTGGG GTCATCTTCT 
AAGAAGGAAA GAGATAGACA GAAGCAAAAG AGCATGTCTC TCTTGAGTCA GCAGCCTTTC 
CTTTCTTTGG TACTTACTTG CCTTAAGGGA CAGGATGAGC AGCGGGAAGG GCTTCTAACA 
TCACTGCAGA ATCAAGTCAA TCAGATCCTT AGTAACTGGA GGGAAGAACG GTACCAGGGT 
GATGTTAAAG CTAGGCAAAT GATGCATGAA GCCTTACAGC TTCGTCTGAA TTTGGTAGGT 
GGCATGTTTG ATACTGTGCA GAGGAGTACC CAGTGGACCA CAGACTGGGC ACTTCTGCTC 
CTCCAGATAA TCACTTCGGG AACAGTTGAT ATGCAGACCA ACAACGAATT GTTCACAACC 
GTGCTGGATA TGCTGGGTGT CCTCATCAAC GGGACGCTCG CCTCCGACTT GTCCAGTGCT 
TCCCAAGGGG GACCCGAGGA AAACAAAAGG GCTTACATGA ACTTAGTGAA AAAGTTAAAA 
AAGGAGCTGG GAGACAAGCG GTCAGACAGC ATTGACAAGG TTCGCCAGCT GCTCCCTTTG 
CCAAAGCAGA CATGTGATAT TATTACCTGT GAGCCAATGG GATCCTTGAT TGACACCAAG 
GGCAACAAAA TTGCGGGGTT TGACTCCATT GATAAGAAAC AGGGTCTTCA GGTGTCAACA 
AAACAAAAGG TGTCCCCTTG GGATTTGTTT GAAGGTCATA AAAATCCTGC TCCTCTCTCC 
TGGGCCTGGT TTGGTACAGT TCGTGTCGAC AGGAAGGTGA TCAAATACGA AGAGCAGCAG 
CATCTGCTCC TGTACCATGC GCACCCCAAA CCCAAGCCGC GGAGCTACTA CCTCGAGCCC 
CTGCCTCTGC CTCCGGAGGA GGAGGAGGAG GAGGAGCCCA CCACCCCTGT CTCTCAGGAA 
CCAGAAAGGA AGTCAGGAGA TCAGGATCAG GGAAAGCATG CCACAGATGA CGAGAAGAAG 
ACAAAGGGCA GGAAGAGGAA GTCAAAGTCA GGCTCTAAGG CTGATGAATA TCCACAGAAC 
AACTTATACA GAGTGCCTCC CAATTACTCT CCCATTCCCT CACAAATGAT GCAGCATCCT 
CAGTCTGCCC TGTGGGGCTA CACCATCATG GGGCAGCCGC AGCAGCCTGG CTTCTTCGTG 
CAGAACCAGC CCCTCCCTCC AGGTGGTTCC AGGCTGGATC CAACGAGCTC CTTCGTCCCA 
GCCAACACGA AGCAGGCCCT GTCCAACATG CTGCAGCGGC GCTCCGGCAC CGTGCTGCAG 
CCCCCTGCCC TCCACGCAGT CACACCTCAG CAGCAGTTGC TCCAGATGAA ACTCCTGCAG 
CAAGAGCAGC AGCAGCAGCG GCTCCTCAGG CACCAAGCAC AGGCACGGTC TCTCCAGCAG 
GATGTCAGTA GCTCTGCCGT ATCCCTTCAG GATGGCTTTG ACAACATAAT GGGTCAGCCG 
ATGGACCCCG CTGCTCTTTT CACCCCGCAA GTCCGACCCA CGCCACAGCT ACCGCAGTAC 
CCAGGGCTTC AGCAAGCACA GAGCATGCCC CACGGGTACA CGATGTACAG CACCCCGATG 
CCTTTGCAGC AGCAGCAGCA GCCCAGCAGC GTGGTGCTCT CTCCTGGCTA CACCCCGCGG 
GCATACACAG CCCACTCCAG CCCCGTGCTG ATGGAGCGGC TGCGGCCGGG GCAGCCGGGC 
AGCGTGGCTC AGCAGCAGGC AGCTGCCTAC ATGCAGCCCC TGGCGGGTGC TCAGCGGTTG 
ACCCATCAGC CTCTGCAGCC AAACTCATTG ATAGGGGCAG GCCTGGACAG TGCATCAACC 
GCACGTACAC ATCCAACCTT GAGCTCAGCA CAGCTGCCCC AGGAGCAAAT GCGACAGAGG 
CAGCAGGAGA TCCGACAGCA GAGACTCTTC CAGATGCAGC AGGGGCAGCA GCAGCAGACT 
CTTGATCTTC AGCCTGTGCA GCCACAGCAG CCCCTGGTAA GTGGGATGGA TGGTGAGCGT 
GGGGCAGGGG CGTGGGATGA GCGGGTGAAG CTTACAGTGA ATTTGCAGTG CTGTTGTCCA 
TGTTTCCTGG ACATGGCCAT TTTTTCCAGA TATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147323.1
CDS	268..6126
Translation	MLPFLKLKSRNARPLTQILEGMLERHEYLTWILDVLEKIRPTDDDILKLVLPLMLQYSEEFVQSAYLSRRLAYFCARRLSLLLSDGPSLAAAHSPHVIIAPSNPPLAVPSPTAPGPVLSPVQLTCSDFLSCPQHRPLVYGLSCMLQTITLCCPSALVWNYSTNENKNMNPGSPLDLLQVAPSSLPMPGGNSTFNQQVRAKIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRSDSSNSMETLYHKIFWANQNKDNQEAAPSDEAVVMLLCEWAVSCKRSGKHRAMVVAKLLEKRQAEIEAERCGEAEVLDEKESLSSASLTSSSLPVFQNILLRFLDTQAPSLSDPNSDHEKTEFVNLVLLFSEFTRHDVFSHDAYMCTLISRGDLSITATTRPRSPNGETGDEHYSKDHDMKMEDHNIMEHMGTDSGPTSIFDDVDKGDFKADFGSEFPIFSPMPGESCENMNSSLDRRISVASEKSVKRERLREVIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRMILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQEAFPTLETVFTKLQQLSYFDQHQVTSQISSNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRRYHACLILNSEQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYSNVQPSNSNLLWDPEFMLDFIENPSAHSINYSMLGKILSDNAANRYSFVCNALMNVCMGHQDAGRINDIANLCAELTACCTVLSSEWLGVVKALCCSSNHVWGFNDLLCSVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDPDAEPGARMTCRLLLHLFRTPQVCLFPQGAGKLFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAILMLGDAEIGSSSVTPVKNEELPVRGLLDEIAEDEMWASSHALKSRGKAVSIETASLSEYARYVLRTICQQEWVGEHCLKEPERLCTDKDLILDPVLSNKQAQKLLQLICYPHGVKECSEGDNPQRQHIKRILQNLDQWTLRQSWLELQLMIKQCMKEPGSGSVAEMNSLLDNIAKATIEVFQQSADLNNNSSNSGIGLFNPNSVGNADTSNTRQNGKKTFLSSSEWRGVWLVAPLIAKLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQGDVKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMQTNNELFTTVLDMLGVLINGTLASDLSSASQGGPEENKRAYMNLVKKLKKELGDKRSDSIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGHKNPAPLSWAWFGTVRVDRKVIKYEEQQHLLLYHAHPKPKPRSYYLEPLPLPPEEEEEEEPTTPVSQEPERKSGDQDQGKHATDDEKKTKGRKRKSKSGSKADEYPQNNLYRVPPNYSPIPSQMMQHPQSALWGYTIMGQPQQPGFFVQNQPLPPGGSRLDPTSSFVPANTKQALSNMLQRRSGTVLQPPALHAVTPQQQLLQMKLLQQEQQQQRLLRHQAQARSLQQDVSSSAVSLQDGFDNIMGQPMDPAALFTPQVRPTPQLPQYPGLQQAQSMPHGYTMYSTPMPLQQQQQPSSVVLSPGYTPRAYTAHSSPVLMERLRPGQPGSVAQQQAAAYMQPLAGAQRLTHQPLQPNSLIGAGLDSASTARTHPTLSSAQLPQEQMRQRQQEIRQQRLFQMQQGQQQQTLDLQPVQPQQPLVSGMDGERGAGAWDERVKLTVNLQCCCPCFLDMAIFSRYV

Target ORF information:

RefSeq Version	XM_015291837.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mediator complex subunit 12-like (MED12L), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291837.1

ORF Insert Sequence:

ATGCTGCCAT TTCTGAAGCT AAAATCAAGA AACGCCAGGC CACTGACCCA AATATTGGAA 
GGAATGCTTG AGAGACACGA GTACTTGACA TGGATACTGG ATGTTCTGGA AAAAATCAGA 
CCTACTGATG ACGACATTCT TAAGCTGGTG CTGCCACTAA TGTTGCAGTA TTCAGAAGAG 
TTTGTTCAGT CAGCTTACCT GTCCCGCCGC CTTGCTTATT TCTGTGCCAG ACGCCTGTCG 
TTACTGCTGA GCGATGGCCC CAGCCTTGCA GCTGCACATT CTCCTCATGT GATTATTGCA 
CCAAGTAACC CTCCTTTGGC AGTGCCGAGT CCGACTGCAC CCGGTCCTGT GCTGAGCCCT 
GTGCAGCTCA CCTGCTCAGA TTTCCTCTCC TGCCCACAAC ACCGCCCGCT GGTGTATGGG 
CTGAGTTGTA TGCTGCAGAC AATAACTCTT TGTTGCCCAA GTGCCTTGGT GTGGAATTAT 
TCCACAAATG AAAATAAGAA CATGAATCCT GGCTCACCCC TGGATTTGCT TCAAGTTGCT 
CCATCTAGTT TACCTATGCC AGGTGGAAAT TCAACATTTA ATCAGCAGGT TCGGGCAAAG 
ATCTATGAGG TAGAACAGCA GATAAAACAA AGAGGTCGTG CTGTGGAGGT GCGGTGGTCT 
TTTGACAAGT GCCAAGAATC AACTGCAGGG GTGACCATCA GCCGAGTTCT GCATACCTTA 
GAAGTGTTGG ATCGACATTG TTTTGACAGA TCAGATTCCA GCAACTCAAT GGAGACGCTT 
TATCATAAGA TTTTCTGGGC AAACCAGAAT AAAGATAACC AAGAGGCAGC CCCCAGCGAT 
GAGGCCGTGG TGATGCTGCT GTGTGAGTGG GCTGTGAGCT GCAAGCGGTC TGGGAAGCAC 
CGGGCCATGG TGGTGGCCAA ACTCCTGGAG AAGAGGCAAG CAGAAATTGA AGCAGAAAGA 
TGTGGTGAAG CTGAAGTCCT AGATGAAAAG GAATCTCTGT CTTCAGCCTC CTTGACAAGT 
TCCAGTCTTC CTGTTTTCCA GAACATCCTG CTGAGGTTTT TGGACACACA GGCTCCCTCG 
CTGTCTGACC CAAATAGCGA CCATGAGAAG ACGGAGTTTG TGAATTTGGT GCTGCTGTTT 
TCTGAATTCA CTCGACACGA TGTTTTCTCC CATGATGCAT ACATGTGCAC TTTAATATCA 
CGTGGAGACT TGTCAATCAC TGCAACTACG AGACCACGTT CACCTAATGG GGAAACTGGG 
GATGAACACT ATTCTAAGGA TCATGATATG AAAATGGAGG ATCATAATAT TATGGAGCAC 
ATGGGCACTG ACTCAGGACC CACTAGTATC TTTGATGATG TAGACAAGGG TGACTTTAAG 
GCAGATTTTG GTTCGGAATT TCCAATTTTT TCTCCGATGC CTGGGGAGTC CTGTGAGAAC 
ATGAACTCTT CTTTAGACAG AAGAATTTCT GTTGCCAGTG AGAAGTCAGT CAAGAGGGAA 
AGACTCAGAG AAGTGATTTT TCCATCTAAC TATGACCTCC TTCGTCATCT GCAATATGCA 
ACGCATTTCC CAATACCTCT GGATGAATCC TCAAGCCATG AGTGCAACCA GCGCATGATT 
CTTCTCTATG GTGTTGGCAA GGAGCGTGAT GAAGCAAGAC ATCAGCTGAA GAAGATTACC 
AAAGACATCC TGAAGATTCT GAATAAGAAG AGTACTACTG AAACGGGTGT TGGGGATGAA 
GGGCAAAAAG CCAGGAAGAA CAAACAAGAA GCATTTCCAA CTCTAGAGAC TGTGTTCACA 
AAACTACAGC AGCTGTCATA TTTTGACCAA CATCAGGTGA CATCTCAGAT CTCCAGCAAT 
GTTCTCGAAC AAATAACCAG CTTTGCCTCA GGAACATCCT ATCACCTCCC CTTGGCTCAC 
CACATACAGC TCATCTTTGA TCTCATGGAA CCAGCACTGA ATATCAACGG GCTGATAGAC 
TTTGCAATAC AGCTGCTGAA TGAGCTGAGC GTGGTGGAGG CAGAACTGCT GTTGAAATCC 
TCCGACCTTG TTGGCAGCTA CACCACCAGC TTGTGCCTGT GCATCGTTGC CGTCCTGCGG 
CGTTACCACG CCTGCCTCAT TCTCAACTCG GAGCAAACTG CACAAGTCTT TGAAGGGTTG 
TGTGGAGTGG TGAAGCACGT TGTTAATCCC TCAGAGTGTT CCTCCCCAGA AAGATGTATT 
TTGGCCTACC TCTATGACCT GTATGTGTCC TGCAGCCACC TGCGAAGCAA GTTTGGAGAC 
CTCTTCAGTA GTGCCTGTTC TAAGGTGAAG CAAACGATAT ACAGCAATGT TCAGCCTTCA 
AATTCCAATC TGCTGTGGGA CCCGGAGTTC ATGCTTGATT TTATTGAGAA CCCTTCTGCT 
CACAGCATTA ACTACTCCAT GCTGGGCAAG ATCCTGAGTG ACAACGCAGC CAACCGCTAC 
AGCTTTGTCT GCAATGCTCT AATGAATGTG TGCATGGGGC ACCAAGATGC AGGGAGGATT 
AATGACATAG CAAACCTTTG TGCAGAGCTG ACGGCGTGCT GCACCGTGCT GAGCTCGGAG 
TGGCTGGGGG TTGTCAAAGC TCTTTGCTGC TCTTCCAATC ACGTGTGGGG TTTCAATGAT 
CTGCTCTGCA GCGTGGATGT GAGTGACCTG TCATTCCACG ATTCCCTGGC CACTTTCATT 
GCCATACTGA TTGCCCGGCA GTGCTTTTCT CTGGAAGATG TCGTTCAGCA CGTAGCACTG 
CCTTCTCTTC TTGCAGCTGC CTGTGGGGAC CCAGATGCTG AGCCTGGGGC CAGGATGACT 
TGTCGGCTTC TCCTGCACCT CTTCAGGACA CCCCAGGTCT GCCTCTTTCC CCAGGGAGCA 
GGAAAATTAT TTCCTGGAAT TCGTTCTTCT TGTGATCGTC ACCTCTTGGC TGCTGCTCAC 
AACAGCATAG AAGTGGGAGC TGTGTTTGCA GTCTTAAAAG CAATTTTGAT GCTTGGTGAC 
GCTGAGATCG GCAGCAGCAG TGTCACCCCT GTGAAGAACG AGGAGCTGCC CGTGAGGGGC 
TTGCTGGATG AGATCGCTGA GGATGAAATG TGGGCTTCCT CTCACGCTCT GAAATCACGT 
GGAAAAGCTG TTTCCATAGA AACCGCCAGT TTGAGCGAGT ATGCTAGATA TGTGCTAAGG 
ACCATTTGCC AGCAGGAATG GGTTGGAGAG CACTGTTTAA AAGAACCTGA GAGGCTGTGC 
ACAGATAAAG ATCTAATTTT GGATCCCGTT CTTTCAAACA AGCAAGCACA GAAACTTCTT 
CAGCTTATCT GTTATCCTCA TGGTGTGAAA GAATGCTCCG AGGGGGACAA CCCTCAGAGG 
CAGCACATCA AACGCATACT TCAGAACTTA GACCAGTGGA CTCTGCGGCA GTCTTGGTTG 
GAGCTGCAAC TAATGATCAA GCAGTGCATG AAGGAGCCTG GTTCTGGGTC TGTGGCTGAA 
ATGAACAGCT TGTTGGATAA TATTGCAAAG GCGACAATAG AGGTGTTTCA GCAATCCGCT 
GATCTAAACA ATAACTCTTC TAACTCTGGT ATAGGCCTCT TCAATCCAAA CTCTGTTGGA 
AATGCTGACA CAAGTAATAC AAGACAGAAT GGTAAAAAGA CATTCCTAAG TTCCTCCGAG 
TGGCGAGGAG TGTGGCTGGT GGCTCCCTTG ATTGCAAAAC TACCTACCTC AGTTCAAGGT 
AGGGTCTTGA AAGCTGCGGG AGAGGAGCTG GAGAAAGGTC AGCATTTGGG GTCATCTTCT 
AAGAAGGAAA GAGATAGACA GAAGCAAAAG AGCATGTCTC TCTTGAGTCA GCAGCCTTTC 
CTTTCTTTGG TACTTACTTG CCTTAAGGGA CAGGATGAGC AGCGGGAAGG GCTTCTAACA 
TCACTGCAGA ATCAAGTCAA TCAGATCCTT AGTAACTGGA GGGAAGAACG GTACCAGGGT 
GATGTTAAAG CTAGGCAAAT GATGCATGAA GCCTTACAGC TTCGTCTGAA TTTGGTAGGT 
GGCATGTTTG ATACTGTGCA GAGGAGTACC CAGTGGACCA CAGACTGGGC ACTTCTGCTC 
CTCCAGATAA TCACTTCGGG AACAGTTGAT ATGCAGACCA ACAACGAATT GTTCACAACC 
GTGCTGGATA TGCTGGGTGT CCTCATCAAC GGGACGCTCG CCTCCGACTT GTCCAGTGCT 
TCCCAAGGGG GACCCGAGGA AAACAAAAGG GCTTACATGA ACTTAGTGAA AAAGTTAAAA 
AAGGAGCTGG GAGACAAGCG GTCAGACAGC ATTGACAAGG TTCGCCAGCT GCTCCCTTTG 
CCAAAGCAGA CATGTGATAT TATTACCTGT GAGCCAATGG GATCCTTGAT TGACACCAAG 
GGCAACAAAA TTGCGGGGTT TGACTCCATT GATAAGAAAC AGGGTCTTCA GGTGTCAACA 
AAACAAAAGG TGTCCCCTTG GGATTTGTTT GAAGGTCATA AAAATCCTGC TCCTCTCTCC 
TGGGCCTGGT TTGGTACAGT TCGTGTCGAC AGGAAGGTGA TCAAATACGA AGAGCAGCAG 
CATCTGCTCC TGTACCATGC GCACCCCAAA CCCAAGCCGC GGAGCTACTA CCTCGAGCCC 
CTGCCTCTGC CTCCGGAGGA GGAGGAGGAG GAGGAGCCCA CCACCCCTGT CTCTCAGGAA 
CCAGAAAGGA AGTCAGGAGA TCAGGATCAG GGAAAGCATG CCACAGATGA CGAGAAGAAG 
ACAAAGGGCA GGAAGAGGAA GTCAAAGTCA GGCTCTAAGG CTGATGAATA TCCACAGAAC 
AACTTATACA GAGTGCCTCC CAATTACTCT CCCATTCCCT CACAAATGAT GCAGCATCCT 
CAGTCTGCCC TGTGGGGCTA CACCATCATG GGGCAGCCGC AGCAGCCTGG CTTCTTCGTG 
CAGAACCAGC CCCTCCCTCC AGGTGGTTCC AGGCTGGATC CAACGAGCTC CTTCGTCCCA 
GCCAACACGA AGCAGGCCCT GTCCAACATG CTGCAGCGGC GCTCCGGCAC CGTGCTGCAG 
CCCCCTGCCC TCCACGCAGT CACACCTCAG CAGCAGTTGC TCCAGATGAA ACTCCTGCAG 
CAAGAGCAGC AGCAGCAGCG GCTCCTCAGG CACCAAGCAC AGGCACGGTC TCTCCAGCAG 
GATGTCAGTA GCTCTGCCGT ATCCCTTCAG GATGGCTTTG ACAACATAAT GGGTCAGCCG 
ATGGACCCCG CTGCTCTTTT CACCCCGCAA GTCCGACCCA CGCCACAGCT ACCGCAGTAC 
CCAGGGCTTC AGCAAGCACA GAGCATGCCC CACGGGTACA CGATGTACAG CACCCCGATG 
CCTTTGCAGC AGCAGCAGCA GCCCAGCAGC GTGGTGCTCT CTCCTGGCTA CACCCCGCGG 
GCATACACAG CCCACTCCAG CCCCGTGCTG ATGGAGCGGC TGCGGCCGGG GCAGCCGGGC 
AGCGTGGCTC AGCAGCAGGC AGCTGCCTAC ATGCAGCCCC TGGCGGGTGC TCAGCGGTTG 
ACCCATCAGC CTCTGCAGCC AAACTCATTG ATAGGGGCAG GCCTGGACAG TGCATCAACC 
GCACGTACAC ATCCAACCTT GAGCTCAGCA CAGCTGCCCC AGGAGCAAAT GCGACAGAGG 
CAGCAGGAGA TCCGACAGCA GAGACTCTTC CAGATGCAGC AGGGGCAGCA GCAGCAGACT 
CTTGATCTTC AGCCTGTGCA GCCACAGCAG CCCCTGGTAA GTGGGATGGA TGGTGAGCGT 
GGGGCAGGGG CGTGGGATGA GCGGGTGAAG CTTACAGTGA ATTTGCAGTG CTGTTGTCCA 
TGTTTCCTGG ACATGGCCAT TTTTTCCAGA TATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MED12L
Entrez Gene ID	425036
Full Name	mediator complex subunit 12 like
General protein information	Preferred Names mediator complex subunit 12 like Names mediator of RNA polymerase II transcription subunit 12-like protein mediator of RNA polymerase II transcription, subunit 12 homolog (S. cerevisiae)-like
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Homo sapiens (human)	MED12L	NP_443728.3
Pan troglodytes (chimpanzee)	MED12L	XP_516821.2
Mus musculus (house mouse)	Med12l	NP_808523.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002523	XP_312414.5
Drosophila melanogaster (fruit fly)	kto	NP_524786.1
	MED12L	XP_001107022.2
Gallus gallus (chicken)	MED12L	XP_422838.4
Canis lupus familiaris (dog)	MED12L	XP_005634712.1
Bos taurus (cattle)	MED12L	NP_001193434.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MED12L cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

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