PLCH1 gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_015291818.1	XP_015147304.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
XM_422832.4	XP_422832.3	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X6
XM_015291816.2	XP_015147302.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_025153768.1	XP_025009536.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_015291820.2	XP_015147306.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X4
XM_025153771.1	XP_025009539.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X3
XM_025153773.1	XP_025009541.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X8
XM_025153774.1	XP_025009542.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X9
XM_015291816.1	XP_015147302.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_015291820.1	XP_015147306.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X4
XM_004943562.2	XP_004943619.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X9
XM_025153775.1	XP_025009543.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X10
XM_015291817.1	XP_015147303.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_015291819.1	XP_015147305.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X3
XM_015291821.1	XP_015147307.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X5
XM_015291822.1	XP_015147308.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X7
XM_015291817.2	XP_015147303.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_025153769.1	XP_025009537.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_015291821.2	XP_015147307.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X5
XM_015291823.1	XP_015147309.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X8
XM_025153770.1	XP_025009538.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
XM_422832.5	XP_422832.3	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X6
XM_025153772.1	XP_025009540.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa152878	XM_025153775.1 Latest version!	Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa40157	XM_015291816.2 Latest version!	Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa40157	XM_015291817.2 Latest version!	Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa40157	XM_025153768.1 Latest version!	Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa40157	XM_025153769.1 Latest version!	Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa40158	XM_025153770.1 Latest version!	Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa40158	XM_015291818.1 Latest version!	Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa40159	XM_015291819.1 Latest version!	Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa40159	XM_025153771.1 Latest version!	Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa40160	XM_015291820.2 Latest version!	Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa40161	XM_015291821.2 Latest version!	Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa40162	XM_015291822.1 Latest version!	Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa40162	XM_025153772.1 Latest version!	Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa12866	XM_025153773.1 Latest version!	Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa12866	XM_015291823.1 Latest version!	Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa12867	XM_025153774.1 Latest version!	Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa12867	XM_004943562.2 Latest version!	Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa12868	XM_422832.5 Latest version!	Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
OGa40157	XM_015291816.1	Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa40160	XM_015291820.1	Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa40157	XM_015291817.1	Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa40161	XM_015291821.1	Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa12868	XM_422832.4	Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

PLCH1 ( XM_025153775.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025009543.1 Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OGa152878
Clone ID Related Accession (Same CDS sequence)	XM_025153775.1
Accession Version	XM_025153775.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTTA TGCAGTCTGG GACTCAGATG GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC 
GTTCGTCTCT TCTACCTGGA CGAGCACAGG ACCTGCATCC GATGGAGACC GTCCAGAAAA 
AGTGAAAAGG CAAAAATTGT CATTGATTCT ATTTACAAAG TGACCGAAGG CCGTCAGTCT 
GAAATCTTCC ACCGCCATGC AGAGGGGAGC TTTGACCCGA GCTGTTGCTT TACCATTTAC 
CATGGCAACC ACCTGGAGTC ACTGGATCTG ATTACCTCTA ACCCAGAGGA AGCTCGCACC 
TGGATCACAG GGCTGAAATA TTTGATGGCT GGGATAAGCG ATGAGGACTC GCTTGCTAAG 
CGACAGAGAA CTCATGATCA CTGGGTGAAA CAAACTTTTG AGGAAGCTGA CAAGAATGGA 
GATGGCCTGC TGAACATTGA AGAGATCCAC CAGCTGATGC ATAAGCTAAA TGTTAACCTG 
CCCCGCAGGA AGGTCCGGCA GATGTTTCAG GAAGCTGACA CAGATGAGAA CCAAGGAACT 
CTAAATTTTG AAGAATTTTC AGTGTTTTAC AAGATGATGT CCTTGCGTCG AGACCTGTAC 
TTACTGCTCT TAAGCTACAG TGACAAGAAA GATCACCTCA CCGTTGAAGA ACTTGCTCAA 
TTTCTGAAGG TGGAACAAAA GATGAGTAAC GTGACGCCAG AATATTGTCT TGACATCATA 
CAGAAGTTTG AAGTATCAGA AGAAAACAAG GAGCAGAACG TGCTTGGGAT TGAAGGTTTC 
ACCAACTTCA TGCGCAGTCC TGCTTGTGAT GTTTTTAATC CATTGCATTG CGAAGTGCAT 
CAGGATATGG ATCAACCCCT TTGTAACTAC TTCATTGCTT CATCTCACAA CACGTACCTG 
ACAGGAGACC AGCTGCTGTC CCAGTCTAGG GTGGAGATGT ATGCACGAGT GCTGCAGGAC 
GGTTGTCGAT GTATTGAAGT GGATTGCTGG GATGGTCCAG ATGGAGAGCC AGTAGTGCAT 
CATGGGTATA CGCTTACCTC TAAAATATTC TTCCGTGACG TGGCAGAAAC TATCAATAAG 
TATGCCTTCA TCAAAAATGA GTTTCCGGTG ATACTGTCTA TTGAAAATCA TTGCAGTATT 
CAGCAGCAAA AGAAGATTGC TCAGTACTTG AAGGAGATAC TTGGTGACAA ATTGGACTTG 
TCATCTGTGG TCACCGGAGA TTCCAGGCAG CTTCCTAGCC CTCAAAACTT GAAAGGGAAA 
ATCCTAGTGA AGGGAAAAAA GTTGCCATAC AGTCTTGGAG CAGATGCTGA GGAAGGAGAA 
GTTTCTGATG AGGACAGTGC TGATGAAATT GAAGACGACT GCAAGCTGAA ATCCTGCTAT 
AGTAACGGTG CAACTGAGCA TCAGGTGGAA TCTTTTATAA GAACAAAGCT TGAATCCCTG 
CTAAAGGAGT CCCAGATCAG GGACAAAGAA GATCCTGACA GCTTCACGGT GAGAGCACTG 
CTAAGGGCAA CTCACGAGGG TTTAAGTGTT AACCTGAAGC AGAACCTGGA CACAAAAGAA 
GGTGGGAAGA AGTCTCATAG TCGATCATTA ATGGGCAACT TTGGTAAACA CAAAAAAACA 
GGGAAGGCAA CATCCAAATG TAGCACATCA GATGATGATG AAAATCAGCA AAACTCTTCT 
GGAAAGGAAG CAGGACAGCT GCACAGTAGA CTGACTCGTC GAAGGAAAAC TGTGAAGCTG 
TGCCGAGCTC TGTCTGACCT AGTGGTGTAC ACGAACTCTG TGGCAGCCCA GGATATAGTA 
GATGATGGAT CAACAGGGAA TGTGCTGTCA TTCAGTGAAA CAAGAGCCCA CCAAGCAGTG 
CAGCAGAAGG CTGAACAGTT CATGCTTTAC AACCAGAAAC AGCTTACAAG GGTTTATCCA 
TCAGCGTATC GGATTGACTC CAGCAATTTC AATCCTTTAC CTTACTGGAA TGTTGGTTGC 
CAGCTAGTGG CACTAAACTA CCAGTCTGAG GGACGTATGA TGCAATTAAA CGAAGCAAAA 
TTTAGGGTAA ATGGCAACTG TGGTTACGTC CTCAAACCTC AGCAGATGTG CAAAGGCTCA 
TTCAACCCCT ATTCTTCAGA TCCACTTCCT GCTAGTCCTA AGAAGCAGCT CATCCTGAAA 
ATCATCAGTG GACAGCAGCT GCCTAAGCCT CCTGACTCAA TGCTAGGAGA CAGAGGAGAG 
ATAATAGATC CCTTTGTCGA GGTGGAAATT ATTGGGTTAC CTGTTGACTG CTGTAAAGAT 
CAGACCAGGG TGGTTGATGA CAATGGGTTT AATCCTGTTT GGGAGGAAAC GCTCACTTTT 
ACCATCCATA TGCCTGAAAT AGCTTTGGTT CGATTTCTTG TTTGGGATCA TGACCCCATT 
GGGCGAGACT TCGTTGGACA AAGAACGCTG GCCTTTAGCA GTCTTGTACC TGGTTATCGA 
CATGTGTATT TAGAAGGGCT GACAGAAGCT TCCATATTTG TTCACATAAC CATTAATGAA 
ATCTATGGGA AGTGGAGCCC CTTAATCCTC AATCCCAGTT ACACAATATT GCACTTTCTA 
GGAGCCACAA AGAGTAAGCA ACTGATCGGA CTCCGAGGGC TGTTTAACAA GAACCCAAAG 
CACAGTTCTG CTGAAAGCAG CGGTCATTAT GTACGGAAGC GTTCTATTGG TGACCGAATT 
CTCCGGCGCA CGGCCAGTGC TCCAGCAAAA GGTAGAAAGA AAAGCAAGAT GGGTTTTCTG 
GAAGCTTCTG AAATTAAAGA CAGTGCATCC GAACCAGCAG ACCTGAAGGA CAAAGAAGGA 
GTTGTAAGGC GTACAAGCCG GAGTTTGCAA GCACGTCCTG CTTCTATGCC AGTGGACAAA 
TTCCTTCTTG TAGGACTGCC GTGCACAGAA GATGAAACTG CTCAAGATGC CAAAGGAAAA 
GACAGTGCAA CTGCCAACAG TGATGGCGAT AAAACTGAGA AGGAAACAAA CTTGAAGGAA 
TCTCTTTTCA CGTGTTCTGA GAAAACTGCA GATCATTCAA ACTTGGCATC TCCCTTGAAA 
AAGGAGAGCG CCCAGAAGGA AACTACAGCT GATTTTTCTC TTGTACCCCA GGAGCAGCCC 
GAGCATTCGG TTGCTGTAAG TGATGTAACC GAAACAAACC GCCCCATAAG CAATCAGGAA 
AGTCTCCTTC CTGCTGAAAC GGTGCCTCTG ATTCCGGACT CTGCCTTTGA GCGCATCACA 
GAAAAAATAC AGGGCAAGAG CTGTGCAGGC AGTAGAAGGG CAGATGACAC AAACAGGTGT 
GAGGAGAAAA TAACTCCTGT CAGGACTTGC CTTCCTGAAA AAGTAGAGGA GGTTGAAAAT 
CTCAAATACG ACATCATCCA CAGAAGCACT GTGGCGTGTC TTTCTGTGAC AGATAGTTCT 
CCTGCCGTCT ACTCTGATGC TCCTGGTTTA TGCTCCTCTT CAACCATGCA AGACAGCGAC 
ATCTCCCGCC TCATAGACGA AGTTTCTTTG GTGACTGAGA GCGAGATGGA CAGCGCTGTC 
TCAGCTCTGA TCGGCCAGCT GGATGTCACC GATGACCAAA CCAGCCTCAC CGCACCGATG 
CTCCCTGGCG CTGGCAGCCG GGCCTTCAGT GCTGTGGCCA CACGCACACT GACTGCAGAG 
CTCAGCACTC CCTGTAAGAA GGACTTCATT GCCACACAGT CCAGCAAGTC CCCGTTATTT 
TCAACTCCAG ACTCTGCCGT GTTCCCCAGC CCCGAGACTG CGGAGTATTC TGTGTACACA 
ATTATCCACG AGGCAACTCT TACACCCGGT TCTGAATTTA AGACCCACAG TGGGTTAGTT 
TGCAAGAAGT CAAAGAGTGC AGAAAATAAC TTGCCTAGCT CTTTTTGTTC TTCACCTTTC 
TCTTTGCTGA ATGCAAATCC CCAAAGAAAA TGTGAAACAA GCTGGGAAGC CCCCGGCTCT 
GCGGGTTCTT TTGCTTCCAG TAGCCTCATC TTTGAGGAGA TGCTTGTGGA CCCTCCCATG 
GGCGAGAATT CGGAAAGCAG CAGTCTTGTA GAGTTAGGTG GGGATTCCCA AGACCTCTTG 
GTAACTGCGT GCGCGTACAA AAGGGAAGAT GTGAGCCAAT TGGCTTCCCC TTTAAAACTG 
AGGCAGCAGC AGGACAGCAG GTACTGTGGT GAGAGGACCT TTGGAAACTG TGCAAGTGCT 
GGGCTCTGTG CTGCTGCCCT GGACTGCTCG GATAACTTTA GGACTGCGGG AAGCAAGCTC 
CCTTTTCCAG CCTGTGAAAA TGTTGGTTTT CTACATCATC GTCACACAGA TGAGCAGAAA 
GATTTGATGT GTACCTCCAG GAGGTCCCAA CTCGATGTGC ACTCACCAGT GCTCAGAAGC 
GCGTTGGCTT TCCCACCCTC CCCAGCCATC AAACAGACCA GCCCTTGCAA GTCCAAGAGC 
CTTGGTGACT TGACATCGGA AGATATTTCC TGCAATTTTG AAAGTAAGTA TCAGTACATA 
AGTAGGAGCT TTGTCACATC TGGCATGAGA GACAAGAAGC TCGCTGCCAT GAAGACGATG 
AGACCGCACT CTACGGATGC TTTGACGGAA CAGCTGAGAA AGCTGCTGTC CCTGGACCAG 
GACGACAGCC AGCAGATGCT GTATTCCAGG CGGCTCGACG AGGACTGCCC AAAGGCACTG 
GTCAGAAAGC TGTCGTCCAG GAGCCAGAGC AGAGTGCGGA ACATCGCCAG CCGTGCCAAG 
GAGAGGCAGG AGGCCAGCAA GCAAAAATCT TCCACCGCAG GCAGCATAGC GGGGGTCGTC 
CTTCGGAACA AGCCTTCGGC GTCCCCTCAC GTCGTCAACA GGCACTCGAC GGGCTCCTAC 
ATAGCCAGGT ACCTGGGCAG CTTCCCTGAG GAGGCAGCAG AGGGCCGGGG CCGACCTGAG 
GGCACGTGTG CTGCCTGGCA CCGCGGCTGT GCTGAGCGGC TGTGTGCGCA CAGCCCCCTG 
CTGCAGCTGG AGCCCGACGG CGACGAGAAG CCTGAAGTCT ATTTCCTGCT GAGGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025009543.1
CDS	313..5352
Translation	MSVMQSGTQMVKLKSGTKGLVRLFYLDEHRTCIRWRPSRKSEKAKIVIDSIYKVTEGRQSEIFHRHAEGSFDPSCCFTIYHGNHLESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDHWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLNFEEFSVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTPEYCLDIIQKFEVSEENKEQNVLGIEGFTNFMRSPACDVFNPLHCEVHQDMDQPLCNYFIASSHNTYLTGDQLLSQSRVEMYARVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKIFFRDVAETINKYAFIKNEFPVILSIENHCSIQQQKKIAQYLKEILGDKLDLSSVVTGDSRQLPSPQNLKGKILVKGKKLPYSLGADAEEGEVSDEDSADEIEDDCKLKSCYSNGATEHQVESFIRTKLESLLKESQIRDKEDPDSFTVRALLRATHEGLSVNLKQNLDTKEGGKKSHSRSLMGNFGKHKKTGKATSKCSTSDDDENQQNSSGKEAGQLHSRLTRRRKTVKLCRALSDLVVYTNSVAAQDIVDDGSTGNVLSFSETRAHQAVQQKAEQFMLYNQKQLTRVYPSAYRIDSSNFNPLPYWNVGCQLVALNYQSEGRMMQLNEAKFRVNGNCGYVLKPQQMCKGSFNPYSSDPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTIHMPEIALVRFLVWDHDPIGRDFVGQRTLAFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSKQLIGLRGLFNKNPKHSSAESSGHYVRKRSIGDRILRRTASAPAKGRKKSKMGFLEASEIKDSASEPADLKDKEGVVRRTSRSLQARPASMPVDKFLLVGLPCTEDETAQDAKGKDSATANSDGDKTEKETNLKESLFTCSEKTADHSNLASPLKKESAQKETTADFSLVPQEQPEHSVAVSDVTETNRPISNQESLLPAETVPLIPDSAFERITEKIQGKSCAGSRRADDTNRCEEKITPVRTCLPEKVEEVENLKYDIIHRSTVACLSVTDSSPAVYSDAPGLCSSSTMQDSDISRLIDEVSLVTESEMDSAVSALIGQLDVTDDQTSLTAPMLPGAGSRAFSAVATRTLTAELSTPCKKDFIATQSSKSPLFSTPDSAVFPSPETAEYSVYTIIHEATLTPGSEFKTHSGLVCKKSKSAENNLPSSFCSSPFSLLNANPQRKCETSWEAPGSAGSFASSSLIFEEMLVDPPMGENSESSSLVELGGDSQDLLVTACAYKREDVSQLASPLKLRQQQDSRYCGERTFGNCASAGLCAAALDCSDNFRTAGSKLPFPACENVGFLHHRHTDEQKDLMCTSRRSQLDVHSPVLRSALAFPPSPAIKQTSPCKSKSLGDLTSEDISCNFESKYQYISRSFVTSGMRDKKLAAMKTMRPHSTDALTEQLRKLLSLDQDDSQQMLYSRRLDEDCPKALVRKLSSRSQSRVRNIASRAKERQEASKQKSSTAGSIAGVVLRNKPSASPHVVNRHSTGSYIARYLGSFPEEAAEGRGRPEGTCAAWHRGCAERLCAHSPLLQLEPDGDEKPEVYFLLRL

Target ORF information:

RefSeq Version	XM_025153775.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025153775.1

ORF Insert Sequence:

ATGAGTGTTA TGCAGTCTGG GACTCAGATG GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC 
GTTCGTCTCT TCTACCTGGA CGAGCACAGG ACCTGCATCC GATGGAGACC GTCCAGAAAA 
AGTGAAAAGG CAAAAATTGT CATTGATTCT ATTTACAAAG TGACCGAAGG CCGTCAGTCT 
GAAATCTTCC ACCGCCATGC AGAGGGGAGC TTTGACCCGA GCTGTTGCTT TACCATTTAC 
CATGGCAACC ACCTGGAGTC ACTGGATCTG ATTACCTCTA ACCCAGAGGA AGCTCGCACC 
TGGATCACAG GGCTGAAATA TTTGATGGCT GGGATAAGCG ATGAGGACTC GCTTGCTAAG 
CGACAGAGAA CTCATGATCA CTGGGTGAAA CAAACTTTTG AGGAAGCTGA CAAGAATGGA 
GATGGCCTGC TGAACATTGA AGAGATCCAC CAGCTGATGC ATAAGCTAAA TGTTAACCTG 
CCCCGCAGGA AGGTCCGGCA GATGTTTCAG GAAGCTGACA CAGATGAGAA CCAAGGAACT 
CTAAATTTTG AAGAATTTTC AGTGTTTTAC AAGATGATGT CCTTGCGTCG AGACCTGTAC 
TTACTGCTCT TAAGCTACAG TGACAAGAAA GATCACCTCA CCGTTGAAGA ACTTGCTCAA 
TTTCTGAAGG TGGAACAAAA GATGAGTAAC GTGACGCCAG AATATTGTCT TGACATCATA 
CAGAAGTTTG AAGTATCAGA AGAAAACAAG GAGCAGAACG TGCTTGGGAT TGAAGGTTTC 
ACCAACTTCA TGCGCAGTCC TGCTTGTGAT GTTTTTAATC CATTGCATTG CGAAGTGCAT 
CAGGATATGG ATCAACCCCT TTGTAACTAC TTCATTGCTT CATCTCACAA CACGTACCTG 
ACAGGAGACC AGCTGCTGTC CCAGTCTAGG GTGGAGATGT ATGCACGAGT GCTGCAGGAC 
GGTTGTCGAT GTATTGAAGT GGATTGCTGG GATGGTCCAG ATGGAGAGCC AGTAGTGCAT 
CATGGGTATA CGCTTACCTC TAAAATATTC TTCCGTGACG TGGCAGAAAC TATCAATAAG 
TATGCCTTCA TCAAAAATGA GTTTCCGGTG ATACTGTCTA TTGAAAATCA TTGCAGTATT 
CAGCAGCAAA AGAAGATTGC TCAGTACTTG AAGGAGATAC TTGGTGACAA ATTGGACTTG 
TCATCTGTGG TCACCGGAGA TTCCAGGCAG CTTCCTAGCC CTCAAAACTT GAAAGGGAAA 
ATCCTAGTGA AGGGAAAAAA GTTGCCATAC AGTCTTGGAG CAGATGCTGA GGAAGGAGAA 
GTTTCTGATG AGGACAGTGC TGATGAAATT GAAGACGACT GCAAGCTGAA ATCCTGCTAT 
AGTAACGGTG CAACTGAGCA TCAGGTGGAA TCTTTTATAA GAACAAAGCT TGAATCCCTG 
CTAAAGGAGT CCCAGATCAG GGACAAAGAA GATCCTGACA GCTTCACGGT GAGAGCACTG 
CTAAGGGCAA CTCACGAGGG TTTAAGTGTT AACCTGAAGC AGAACCTGGA CACAAAAGAA 
GGTGGGAAGA AGTCTCATAG TCGATCATTA ATGGGCAACT TTGGTAAACA CAAAAAAACA 
GGGAAGGCAA CATCCAAATG TAGCACATCA GATGATGATG AAAATCAGCA AAACTCTTCT 
GGAAAGGAAG CAGGACAGCT GCACAGTAGA CTGACTCGTC GAAGGAAAAC TGTGAAGCTG 
TGCCGAGCTC TGTCTGACCT AGTGGTGTAC ACGAACTCTG TGGCAGCCCA GGATATAGTA 
GATGATGGAT CAACAGGGAA TGTGCTGTCA TTCAGTGAAA CAAGAGCCCA CCAAGCAGTG 
CAGCAGAAGG CTGAACAGTT CATGCTTTAC AACCAGAAAC AGCTTACAAG GGTTTATCCA 
TCAGCGTATC GGATTGACTC CAGCAATTTC AATCCTTTAC CTTACTGGAA TGTTGGTTGC 
CAGCTAGTGG CACTAAACTA CCAGTCTGAG GGACGTATGA TGCAATTAAA CGAAGCAAAA 
TTTAGGGTAA ATGGCAACTG TGGTTACGTC CTCAAACCTC AGCAGATGTG CAAAGGCTCA 
TTCAACCCCT ATTCTTCAGA TCCACTTCCT GCTAGTCCTA AGAAGCAGCT CATCCTGAAA 
ATCATCAGTG GACAGCAGCT GCCTAAGCCT CCTGACTCAA TGCTAGGAGA CAGAGGAGAG 
ATAATAGATC CCTTTGTCGA GGTGGAAATT ATTGGGTTAC CTGTTGACTG CTGTAAAGAT 
CAGACCAGGG TGGTTGATGA CAATGGGTTT AATCCTGTTT GGGAGGAAAC GCTCACTTTT 
ACCATCCATA TGCCTGAAAT AGCTTTGGTT CGATTTCTTG TTTGGGATCA TGACCCCATT 
GGGCGAGACT TCGTTGGACA AAGAACGCTG GCCTTTAGCA GTCTTGTACC TGGTTATCGA 
CATGTGTATT TAGAAGGGCT GACAGAAGCT TCCATATTTG TTCACATAAC CATTAATGAA 
ATCTATGGGA AGTGGAGCCC CTTAATCCTC AATCCCAGTT ACACAATATT GCACTTTCTA 
GGAGCCACAA AGAGTAAGCA ACTGATCGGA CTCCGAGGGC TGTTTAACAA GAACCCAAAG 
CACAGTTCTG CTGAAAGCAG CGGTCATTAT GTACGGAAGC GTTCTATTGG TGACCGAATT 
CTCCGGCGCA CGGCCAGTGC TCCAGCAAAA GGTAGAAAGA AAAGCAAGAT GGGTTTTCTG 
GAAGCTTCTG AAATTAAAGA CAGTGCATCC GAACCAGCAG ACCTGAAGGA CAAAGAAGGA 
GTTGTAAGGC GTACAAGCCG GAGTTTGCAA GCACGTCCTG CTTCTATGCC AGTGGACAAA 
TTCCTTCTTG TAGGACTGCC GTGCACAGAA GATGAAACTG CTCAAGATGC CAAAGGAAAA 
GACAGTGCAA CTGCCAACAG TGATGGCGAT AAAACTGAGA AGGAAACAAA CTTGAAGGAA 
TCTCTTTTCA CGTGTTCTGA GAAAACTGCA GATCATTCAA ACTTGGCATC TCCCTTGAAA 
AAGGAGAGCG CCCAGAAGGA AACTACAGCT GATTTTTCTC TTGTACCCCA GGAGCAGCCC 
GAGCATTCGG TTGCTGTAAG TGATGTAACC GAAACAAACC GCCCCATAAG CAATCAGGAA 
AGTCTCCTTC CTGCTGAAAC GGTGCCTCTG ATTCCGGACT CTGCCTTTGA GCGCATCACA 
GAAAAAATAC AGGGCAAGAG CTGTGCAGGC AGTAGAAGGG CAGATGACAC AAACAGGTGT 
GAGGAGAAAA TAACTCCTGT CAGGACTTGC CTTCCTGAAA AAGTAGAGGA GGTTGAAAAT 
CTCAAATACG ACATCATCCA CAGAAGCACT GTGGCGTGTC TTTCTGTGAC AGATAGTTCT 
CCTGCCGTCT ACTCTGATGC TCCTGGTTTA TGCTCCTCTT CAACCATGCA AGACAGCGAC 
ATCTCCCGCC TCATAGACGA AGTTTCTTTG GTGACTGAGA GCGAGATGGA CAGCGCTGTC 
TCAGCTCTGA TCGGCCAGCT GGATGTCACC GATGACCAAA CCAGCCTCAC CGCACCGATG 
CTCCCTGGCG CTGGCAGCCG GGCCTTCAGT GCTGTGGCCA CACGCACACT GACTGCAGAG 
CTCAGCACTC CCTGTAAGAA GGACTTCATT GCCACACAGT CCAGCAAGTC CCCGTTATTT 
TCAACTCCAG ACTCTGCCGT GTTCCCCAGC CCCGAGACTG CGGAGTATTC TGTGTACACA 
ATTATCCACG AGGCAACTCT TACACCCGGT TCTGAATTTA AGACCCACAG TGGGTTAGTT 
TGCAAGAAGT CAAAGAGTGC AGAAAATAAC TTGCCTAGCT CTTTTTGTTC TTCACCTTTC 
TCTTTGCTGA ATGCAAATCC CCAAAGAAAA TGTGAAACAA GCTGGGAAGC CCCCGGCTCT 
GCGGGTTCTT TTGCTTCCAG TAGCCTCATC TTTGAGGAGA TGCTTGTGGA CCCTCCCATG 
GGCGAGAATT CGGAAAGCAG CAGTCTTGTA GAGTTAGGTG GGGATTCCCA AGACCTCTTG 
GTAACTGCGT GCGCGTACAA AAGGGAAGAT GTGAGCCAAT TGGCTTCCCC TTTAAAACTG 
AGGCAGCAGC AGGACAGCAG GTACTGTGGT GAGAGGACCT TTGGAAACTG TGCAAGTGCT 
GGGCTCTGTG CTGCTGCCCT GGACTGCTCG GATAACTTTA GGACTGCGGG AAGCAAGCTC 
CCTTTTCCAG CCTGTGAAAA TGTTGGTTTT CTACATCATC GTCACACAGA TGAGCAGAAA 
GATTTGATGT GTACCTCCAG GAGGTCCCAA CTCGATGTGC ACTCACCAGT GCTCAGAAGC 
GCGTTGGCTT TCCCACCCTC CCCAGCCATC AAACAGACCA GCCCTTGCAA GTCCAAGAGC 
CTTGGTGACT TGACATCGGA AGATATTTCC TGCAATTTTG AAAGTAAGTA TCAGTACATA 
AGTAGGAGCT TTGTCACATC TGGCATGAGA GACAAGAAGC TCGCTGCCAT GAAGACGATG 
AGACCGCACT CTACGGATGC TTTGACGGAA CAGCTGAGAA AGCTGCTGTC CCTGGACCAG 
GACGACAGCC AGCAGATGCT GTATTCCAGG CGGCTCGACG AGGACTGCCC AAAGGCACTG 
GTCAGAAAGC TGTCGTCCAG GAGCCAGAGC AGAGTGCGGA ACATCGCCAG CCGTGCCAAG 
GAGAGGCAGG AGGCCAGCAA GCAAAAATCT TCCACCGCAG GCAGCATAGC GGGGGTCGTC 
CTTCGGAACA AGCCTTCGGC GTCCCCTCAC GTCGTCAACA GGCACTCGAC GGGCTCCTAC 
ATAGCCAGGT ACCTGGGCAG CTTCCCTGAG GAGGCAGCAG AGGGCCGGGG CCGACCTGAG 
GGCACGTGTG CTGCCTGGCA CCGCGGCTGT GCTGAGCGGC TGTGTGCGCA CAGCCCCCTG 
CTGCAGCTGG AGCCCGACGG CGACGAGAAG CCTGAAGTCT ATTTCCTGCT GAGGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_015291816.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147302.1 Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.

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CloneID	OGa40157
Clone ID Related Accession (Same CDS sequence)	XM_015291816.2 , XM_025153768.1 , XM_015291816.1 , XM_015291817.1 , XM_015291817.2 , XM_025153769.1
Accession Version	XM_015291816.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291816.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGTAGA 
CTGACTCGTC GAAGGAAAAC TGTGAAGCTG TGCCGAGCTC TGTCTGACCT AGTGGTGTAC 
ACGAACTCTG TGGCAGCCCA GGATATAGTA GATGATGGAT CAACAGGGAA TGTGCTGTCA 
TTCAGTGAAA CAAGAGCCCA CCAAGCAGTG CAGCAGAAGG CTGAACAGTT CATGCTTTAC 
AACCAGAAAC AGCTTACAAG GGTTTATCCA TCAGCGTATC GGATTGACTC CAGCAATTTC 
AATCCTTTAC CTTACTGGAA TGTTGGTTGC CAGCTAGTGG CACTAAACTA CCAGTCTGAG 
GGACGTATGA TGCAATTAAA CGAAGCAAAA TTTAGGGTAA ATGGCAACTG TGGTTACGTC 
CTCAAACCTC AGCAGATGTG CAAAGGCTCA TTCAACCCCT ATTCTTCAGA TCCACTTCCT 
GCTAGTCCTA AGAAGCAGCT CATCCTGAAA ATCATCAGTG GACAGCAGCT GCCTAAGCCT 
CCTGACTCAA TGCTAGGAGA CAGAGGAGAG ATAATAGATC CCTTTGTCGA GGTGGAAATT 
ATTGGGTTAC CTGTTGACTG CTGTAAAGAT CAGACCAGGG TGGTTGATGA CAATGGGTTT 
AATCCTGTTT GGGAGGAAAC GCTCACTTTT ACCATCCATA TGCCTGAAAT AGCTTTGGTT 
CGATTTCTTG TTTGGGATCA TGACCCCATT GGGCGAGACT TCGTTGGACA AAGAACGCTG 
GCCTTTAGCA GTCTTGTACC TGGTTATCGA CATGTGTATT TAGAAGGGCT GACAGAAGCT 
TCCATATTTG TTCACATAAC CATTAATGAA ATCTATGGGA AGTGGAGCCC CTTAATCCTC 
AATCCCAGTT ACACAATATT GCACTTTCTA GGAGCCACAA AGAGTAAGCA ACTGATCGGA 
CTCCGAGGGC TGTTTAACAA GAACCCAAAG CACAGTTCTG CTGAAAGCAG CGGTCATTAT 
GTACGGAAGC GTTCTATTGG TGACCGAATT CTCCGGCGCA CGGCCAGTGC TCCAGCAAAA 
GGTAGAAAGA AAAGCAAGAT GGGTTTTCTG GAAGCTTCTG AAATTAAAGA CAGTGCATCC 
GAACCAGCAG ACCTGAAGGA CAAAGAAGGA GTTGTAAGGC GTACAAGCCG GAGTTTGCAA 
GCACGTCCTG CTTCTATGCC AGTGGACAAA TTCCTTCTTG TAGGACTGCC GTGCACAGAA 
GATGAAACTG CTCAAGATGC CAAAGGAAAA GACAGTGCAA CTGCCAACAG TGATGGCGAT 
AAAACTGAGA AGGAAACAAA CTTGAAGGAA TCTCTTTTCA CGTGTTCTGA GAAAACTGCA 
GATCATTCAA ACTTGGCATC TCCCTTGAAA AAGGAGAGCG CCCAGAAGGA AACTACAGCT 
GATTTTTCTC TTGTACCCCA GGAGCAGCCC GAGCATTCGG TTGCTGTAAG TGATGTAACC 
GAAACAAACC GCCCCATAAG CAATCAGGAA AGTCTCCTTC CTGCTGAAAC GGTGCCTCTG 
ATTCCGGACT CTGCCTTTGA GCGCATCACA GAAAAAATAC AGGGCAAGAG CTGTGCAGGC 
AGTAGAAGGG CAGATGACAC AAACAGGTGT GAGGAGAAAA TAACTCCTGT CAGGACTTGC 
CTTCCTGAAA AAGTAGAGGA GGTTGAAAAT CTCAAATACG ACATCATCCA CAGAAGCACT 
GTGGCGTGTC TTTCTGTGAC AGATAGTTCT CCTGCCGTCT ACTCTGATGC TCCTGGTTTA 
TGCTCCTCTT CAACCATGCA AGACAGCGAC ATCTCCCGCC TCATAGACGA AGTTTCTTTG 
GTGACTGAGA GCGAGATGGA CAGCGCTGTC TCAGCTCTGA TCGGCCAGCT GGATGTCACC 
GATGACCAAA CCAGCCTCAC CGCACCGATG CTCCCTGGCG CTGGCAGCCG GGCCTTCAGT 
GCTGTGGCCA CACGCACACT GACTGCAGAG CTCAGCACTC CCTGTAAGAA GGACTTCATT 
GCCACACAGT CCAGCAAGTC CCCGTTATTT TCAACTCCAG ACTCTGCCGT GTTCCCCAGC 
CCCGAGACTG CGGAGTATTC TGTGTACACA ATTATCCACG AGGCAACTCT TACACCCGGT 
TCTGAATTTA AGACCCACAG TGGGTTAGTT TGCAAGAAGT CAAAGAGTGC AGAAAATAAC 
TTGCCTAGCT CTTTTTGTTC TTCACCTTTC TCTTTGCTGA ATGCAAATCC CCAAAGAAAA 
TGTGAAACAA GCTGGGAAGC CCCCGGCTCT GCGGGTTCTT TTGCTTCCAG TAGCCTCATC 
TTTGAGGAGA TGCTTGTGGA CCCTCCCATG GGCGAGAATT CGGAAAGCAG CAGTCTTGTA 
GAGTTAGGTG GGGATTCCCA AGACCTCTTG GTAACTGCGT GCGCGTACAA AAGGGAAGAT 
GTGAGCCAAT TGGCTTCCCC TTTAAAACTG AGGCAGCAGC AGGACAGCAG GTACTGTGGT 
GAGAGGACCT TTGGAAACTG TGCAAGTGCT GGGCTCTGTG CTGCTGCCCT GGACTGCTCG 
GATAACTTTA GGACTGCGGG AAGCAAGCTC CCTTTTCCAG CCTGTGAAAA TGTTGGTTTT 
CTACATCATC GTCACACAGA TGAGCAGAAA GATTTGATGT GTACCTCCAG GAGGTCCCAA 
CTCGATGTGC ACTCACCAGT GCTCAGAAGC GCGTTGGCTT TCCCACCCTC CCCAGCCATC 
AAACAGACCA GCCCTTGCAA GTCCAAGAGC CTTGGTGACT TGACATCGGA AGATATTTCC 
TGCAATTTTG AAAGTAAGTA TCAGTACATA AGTAGGAGCT TTGTCACATC TGGCATGAGA 
GACAAGAAGC TCGCTGCCAT GAAGACGATG AGACCGCACT CTACGGATGC TTTGACGGAA 
CAGCTGAGAA AGCTGCTGTC CCTGGACCAG GACGACAGCC AGCAGATGCT GTATTCCAGG 
CGGCTCGACG AGGACTGCCC AAAGGCACTG GTCAGAAAGC TGTCGTCCAG GAGCCAGAGC 
AGAGTGCGGA ACATCGCCAG CCGTGCCAAG GAGAGGCAGG AGGCCAGCAA GCAAAAATCT 
TCCACCGCAG GCAGCATAGC GGGGGTCGTC CTTCGGAACA AGCCTTCGGC GTCCCCTCAC 
GTCGTCAACA GGCACTCGAC GGGCTCCTAC ATAGCCAGGT ACCTGGGCAG CTTCCCTGAG 
GAGGCAGCAG AGGGCCGGGG CCGACCTGAG GGCACGTGTG CTGCCTGGCA CCGCGGCTGT 
GCTGAGCGGC TGTGTGCGCA CAGCCCCCTG CTGCAGCTGG AGCCCGACGG CGACGAGAAG 
CCTGAAGTCT ATTTCCTGCT GAGGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147302.1
CDS	1365..6494
Translation	MSYWSLDKKSCLQYCRHFLADNGVFHVERCMSVMQSGTQMVKLKSGTKGLVRLFYLDEHRTCIRWRPSRKSEKAKIVIDSIYKVTEGRQSEIFHRHAEGSFDPSCCFTIYHGNHLESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDHWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLNFEEFSVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTPEYCLDIIQKFEVSEENKEQNVLGIEGFTNFMRSPACDVFNPLHCEVHQDMDQPLCNYFIASSHNTYLTGDQLLSQSRVEMYARVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKIFFRDVAETINKYAFIKNEFPVILSIENHCSIQQQKKIAQYLKEILGDKLDLSSVVTGDSRQLPSPQNLKGKILVKGKKLPYSLGADAEEGEVSDEDSADEIEDDCKLKSCYSNGATEHQVESFIRTKLESLLKESQIRDKEDPDSFTVRALLRATHEGLSVNLKQNLDTKEGGKKSHSRSLMGNFGKHKKTGKATSKCSTSDDDENQQNSSGKEAGQLHSRLTRRRKTVKLCRALSDLVVYTNSVAAQDIVDDGSTGNVLSFSETRAHQAVQQKAEQFMLYNQKQLTRVYPSAYRIDSSNFNPLPYWNVGCQLVALNYQSEGRMMQLNEAKFRVNGNCGYVLKPQQMCKGSFNPYSSDPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTIHMPEIALVRFLVWDHDPIGRDFVGQRTLAFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSKQLIGLRGLFNKNPKHSSAESSGHYVRKRSIGDRILRRTASAPAKGRKKSKMGFLEASEIKDSASEPADLKDKEGVVRRTSRSLQARPASMPVDKFLLVGLPCTEDETAQDAKGKDSATANSDGDKTEKETNLKESLFTCSEKTADHSNLASPLKKESAQKETTADFSLVPQEQPEHSVAVSDVTETNRPISNQESLLPAETVPLIPDSAFERITEKIQGKSCAGSRRADDTNRCEEKITPVRTCLPEKVEEVENLKYDIIHRSTVACLSVTDSSPAVYSDAPGLCSSSTMQDSDISRLIDEVSLVTESEMDSAVSALIGQLDVTDDQTSLTAPMLPGAGSRAFSAVATRTLTAELSTPCKKDFIATQSSKSPLFSTPDSAVFPSPETAEYSVYTIIHEATLTPGSEFKTHSGLVCKKSKSAENNLPSSFCSSPFSLLNANPQRKCETSWEAPGSAGSFASSSLIFEEMLVDPPMGENSESSSLVELGGDSQDLLVTACAYKREDVSQLASPLKLRQQQDSRYCGERTFGNCASAGLCAAALDCSDNFRTAGSKLPFPACENVGFLHHRHTDEQKDLMCTSRRSQLDVHSPVLRSALAFPPSPAIKQTSPCKSKSLGDLTSEDISCNFESKYQYISRSFVTSGMRDKKLAAMKTMRPHSTDALTEQLRKLLSLDQDDSQQMLYSRRLDEDCPKALVRKLSSRSQSRVRNIASRAKERQEASKQKSSTAGSIAGVVLRNKPSASPHVVNRHSTGSYIARYLGSFPEEAAEGRGRPEGTCAAWHRGCAERLCAHSPLLQLEPDGDEKPEVYFLLRL

Target ORF information:

RefSeq Version	XM_015291816.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291816.2

ORF Insert Sequence:

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGTAGA 
CTGACTCGTC GAAGGAAAAC TGTGAAGCTG TGCCGAGCTC TGTCTGACCT AGTGGTGTAC 
ACGAACTCTG TGGCAGCCCA GGATATAGTA GATGATGGAT CAACAGGGAA TGTGCTGTCA 
TTCAGTGAAA CAAGAGCCCA CCAAGCAGTG CAGCAGAAGG CTGAACAGTT CATGCTTTAC 
AACCAGAAAC AGCTTACAAG GGTTTATCCA TCAGCGTATC GGATTGACTC CAGCAATTTC 
AATCCTTTAC CTTACTGGAA TGTTGGTTGC CAGCTAGTGG CACTAAACTA CCAGTCTGAG 
GGACGTATGA TGCAATTAAA CGAAGCAAAA TTTAGGGTAA ATGGCAACTG TGGTTACGTC 
CTCAAACCTC AGCAGATGTG CAAAGGCTCA TTCAACCCCT ATTCTTCAGA TCCACTTCCT 
GCTAGTCCTA AGAAGCAGCT CATCCTGAAA ATCATCAGTG GACAGCAGCT GCCTAAGCCT 
CCTGACTCAA TGCTAGGAGA CAGAGGAGAG ATAATAGATC CCTTTGTCGA GGTGGAAATT 
ATTGGGTTAC CTGTTGACTG CTGTAAAGAT CAGACCAGGG TGGTTGATGA CAATGGGTTT 
AATCCTGTTT GGGAGGAAAC GCTCACTTTT ACCATCCATA TGCCTGAAAT AGCTTTGGTT 
CGATTTCTTG TTTGGGATCA TGACCCCATT GGGCGAGACT TCGTTGGACA AAGAACGCTG 
GCCTTTAGCA GTCTTGTACC TGGTTATCGA CATGTGTATT TAGAAGGGCT GACAGAAGCT 
TCCATATTTG TTCACATAAC CATTAATGAA ATCTATGGGA AGTGGAGCCC CTTAATCCTC 
AATCCCAGTT ACACAATATT GCACTTTCTA GGAGCCACAA AGAGTAAGCA ACTGATCGGA 
CTCCGAGGGC TGTTTAACAA GAACCCAAAG CACAGTTCTG CTGAAAGCAG CGGTCATTAT 
GTACGGAAGC GTTCTATTGG TGACCGAATT CTCCGGCGCA CGGCCAGTGC TCCAGCAAAA 
GGTAGAAAGA AAAGCAAGAT GGGTTTTCTG GAAGCTTCTG AAATTAAAGA CAGTGCATCC 
GAACCAGCAG ACCTGAAGGA CAAAGAAGGA GTTGTAAGGC GTACAAGCCG GAGTTTGCAA 
GCACGTCCTG CTTCTATGCC AGTGGACAAA TTCCTTCTTG TAGGACTGCC GTGCACAGAA 
GATGAAACTG CTCAAGATGC CAAAGGAAAA GACAGTGCAA CTGCCAACAG TGATGGCGAT 
AAAACTGAGA AGGAAACAAA CTTGAAGGAA TCTCTTTTCA CGTGTTCTGA GAAAACTGCA 
GATCATTCAA ACTTGGCATC TCCCTTGAAA AAGGAGAGCG CCCAGAAGGA AACTACAGCT 
GATTTTTCTC TTGTACCCCA GGAGCAGCCC GAGCATTCGG TTGCTGTAAG TGATGTAACC 
GAAACAAACC GCCCCATAAG CAATCAGGAA AGTCTCCTTC CTGCTGAAAC GGTGCCTCTG 
ATTCCGGACT CTGCCTTTGA GCGCATCACA GAAAAAATAC AGGGCAAGAG CTGTGCAGGC 
AGTAGAAGGG CAGATGACAC AAACAGGTGT GAGGAGAAAA TAACTCCTGT CAGGACTTGC 
CTTCCTGAAA AAGTAGAGGA GGTTGAAAAT CTCAAATACG ACATCATCCA CAGAAGCACT 
GTGGCGTGTC TTTCTGTGAC AGATAGTTCT CCTGCCGTCT ACTCTGATGC TCCTGGTTTA 
TGCTCCTCTT CAACCATGCA AGACAGCGAC ATCTCCCGCC TCATAGACGA AGTTTCTTTG 
GTGACTGAGA GCGAGATGGA CAGCGCTGTC TCAGCTCTGA TCGGCCAGCT GGATGTCACC 
GATGACCAAA CCAGCCTCAC CGCACCGATG CTCCCTGGCG CTGGCAGCCG GGCCTTCAGT 
GCTGTGGCCA CACGCACACT GACTGCAGAG CTCAGCACTC CCTGTAAGAA GGACTTCATT 
GCCACACAGT CCAGCAAGTC CCCGTTATTT TCAACTCCAG ACTCTGCCGT GTTCCCCAGC 
CCCGAGACTG CGGAGTATTC TGTGTACACA ATTATCCACG AGGCAACTCT TACACCCGGT 
TCTGAATTTA AGACCCACAG TGGGTTAGTT TGCAAGAAGT CAAAGAGTGC AGAAAATAAC 
TTGCCTAGCT CTTTTTGTTC TTCACCTTTC TCTTTGCTGA ATGCAAATCC CCAAAGAAAA 
TGTGAAACAA GCTGGGAAGC CCCCGGCTCT GCGGGTTCTT TTGCTTCCAG TAGCCTCATC 
TTTGAGGAGA TGCTTGTGGA CCCTCCCATG GGCGAGAATT CGGAAAGCAG CAGTCTTGTA 
GAGTTAGGTG GGGATTCCCA AGACCTCTTG GTAACTGCGT GCGCGTACAA AAGGGAAGAT 
GTGAGCCAAT TGGCTTCCCC TTTAAAACTG AGGCAGCAGC AGGACAGCAG GTACTGTGGT 
GAGAGGACCT TTGGAAACTG TGCAAGTGCT GGGCTCTGTG CTGCTGCCCT GGACTGCTCG 
GATAACTTTA GGACTGCGGG AAGCAAGCTC CCTTTTCCAG CCTGTGAAAA TGTTGGTTTT 
CTACATCATC GTCACACAGA TGAGCAGAAA GATTTGATGT GTACCTCCAG GAGGTCCCAA 
CTCGATGTGC ACTCACCAGT GCTCAGAAGC GCGTTGGCTT TCCCACCCTC CCCAGCCATC 
AAACAGACCA GCCCTTGCAA GTCCAAGAGC CTTGGTGACT TGACATCGGA AGATATTTCC 
TGCAATTTTG AAAGTAAGTA TCAGTACATA AGTAGGAGCT TTGTCACATC TGGCATGAGA 
GACAAGAAGC TCGCTGCCAT GAAGACGATG AGACCGCACT CTACGGATGC TTTGACGGAA 
CAGCTGAGAA AGCTGCTGTC CCTGGACCAG GACGACAGCC AGCAGATGCT GTATTCCAGG 
CGGCTCGACG AGGACTGCCC AAAGGCACTG GTCAGAAAGC TGTCGTCCAG GAGCCAGAGC 
AGAGTGCGGA ACATCGCCAG CCGTGCCAAG GAGAGGCAGG AGGCCAGCAA GCAAAAATCT 
TCCACCGCAG GCAGCATAGC GGGGGTCGTC CTTCGGAACA AGCCTTCGGC GTCCCCTCAC 
GTCGTCAACA GGCACTCGAC GGGCTCCTAC ATAGCCAGGT ACCTGGGCAG CTTCCCTGAG 
GAGGCAGCAG AGGGCCGGGG CCGACCTGAG GGCACGTGTG CTGCCTGGCA CCGCGGCTGT 
GCTGAGCGGC TGTGTGCGCA CAGCCCCCTG CTGCAGCTGG AGCCCGACGG CGACGAGAAG 
CCTGAAGTCT ATTTCCTGCT GAGGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_025153768.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025009536.1 Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X3, mRNA.

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CloneID	OGa40157
Clone ID Related Accession (Same CDS sequence)	XM_015291816.2 , XM_025153768.1 , XM_015291816.1 , XM_015291817.1 , XM_015291817.2 , XM_025153769.1
Accession Version	XM_025153768.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGTAGA 
CTGACTCGTC GAAGGAAAAC TGTGAAGCTG TGCCGAGCTC TGTCTGACCT AGTGGTGTAC 
ACGAACTCTG TGGCAGCCCA GGATATAGTA GATGATGGAT CAACAGGGAA TGTGCTGTCA 
TTCAGTGAAA CAAGAGCCCA CCAAGCAGTG CAGCAGAAGG CTGAACAGTT CATGCTTTAC 
AACCAGAAAC AGCTTACAAG GGTTTATCCA TCAGCGTATC GGATTGACTC CAGCAATTTC 
AATCCTTTAC CTTACTGGAA TGTTGGTTGC CAGCTAGTGG CACTAAACTA CCAGTCTGAG 
GGACGTATGA TGCAATTAAA CGAAGCAAAA TTTAGGGTAA ATGGCAACTG TGGTTACGTC 
CTCAAACCTC AGCAGATGTG CAAAGGCTCA TTCAACCCCT ATTCTTCAGA TCCACTTCCT 
GCTAGTCCTA AGAAGCAGCT CATCCTGAAA ATCATCAGTG GACAGCAGCT GCCTAAGCCT 
CCTGACTCAA TGCTAGGAGA CAGAGGAGAG ATAATAGATC CCTTTGTCGA GGTGGAAATT 
ATTGGGTTAC CTGTTGACTG CTGTAAAGAT CAGACCAGGG TGGTTGATGA CAATGGGTTT 
AATCCTGTTT GGGAGGAAAC GCTCACTTTT ACCATCCATA TGCCTGAAAT AGCTTTGGTT 
CGATTTCTTG TTTGGGATCA TGACCCCATT GGGCGAGACT TCGTTGGACA AAGAACGCTG 
GCCTTTAGCA GTCTTGTACC TGGTTATCGA CATGTGTATT TAGAAGGGCT GACAGAAGCT 
TCCATATTTG TTCACATAAC CATTAATGAA ATCTATGGGA AGTGGAGCCC CTTAATCCTC 
AATCCCAGTT ACACAATATT GCACTTTCTA GGAGCCACAA AGAGTAAGCA ACTGATCGGA 
CTCCGAGGGC TGTTTAACAA GAACCCAAAG CACAGTTCTG CTGAAAGCAG CGGTCATTAT 
GTACGGAAGC GTTCTATTGG TGACCGAATT CTCCGGCGCA CGGCCAGTGC TCCAGCAAAA 
GGTAGAAAGA AAAGCAAGAT GGGTTTTCTG GAAGCTTCTG AAATTAAAGA CAGTGCATCC 
GAACCAGCAG ACCTGAAGGA CAAAGAAGGA GTTGTAAGGC GTACAAGCCG GAGTTTGCAA 
GCACGTCCTG CTTCTATGCC AGTGGACAAA TTCCTTCTTG TAGGACTGCC GTGCACAGAA 
GATGAAACTG CTCAAGATGC CAAAGGAAAA GACAGTGCAA CTGCCAACAG TGATGGCGAT 
AAAACTGAGA AGGAAACAAA CTTGAAGGAA TCTCTTTTCA CGTGTTCTGA GAAAACTGCA 
GATCATTCAA ACTTGGCATC TCCCTTGAAA AAGGAGAGCG CCCAGAAGGA AACTACAGCT 
GATTTTTCTC TTGTACCCCA GGAGCAGCCC GAGCATTCGG TTGCTGTAAG TGATGTAACC 
GAAACAAACC GCCCCATAAG CAATCAGGAA AGTCTCCTTC CTGCTGAAAC GGTGCCTCTG 
ATTCCGGACT CTGCCTTTGA GCGCATCACA GAAAAAATAC AGGGCAAGAG CTGTGCAGGC 
AGTAGAAGGG CAGATGACAC AAACAGGTGT GAGGAGAAAA TAACTCCTGT CAGGACTTGC 
CTTCCTGAAA AAGTAGAGGA GGTTGAAAAT CTCAAATACG ACATCATCCA CAGAAGCACT 
GTGGCGTGTC TTTCTGTGAC AGATAGTTCT CCTGCCGTCT ACTCTGATGC TCCTGGTTTA 
TGCTCCTCTT CAACCATGCA AGACAGCGAC ATCTCCCGCC TCATAGACGA AGTTTCTTTG 
GTGACTGAGA GCGAGATGGA CAGCGCTGTC TCAGCTCTGA TCGGCCAGCT GGATGTCACC 
GATGACCAAA CCAGCCTCAC CGCACCGATG CTCCCTGGCG CTGGCAGCCG GGCCTTCAGT 
GCTGTGGCCA CACGCACACT GACTGCAGAG CTCAGCACTC CCTGTAAGAA GGACTTCATT 
GCCACACAGT CCAGCAAGTC CCCGTTATTT TCAACTCCAG ACTCTGCCGT GTTCCCCAGC 
CCCGAGACTG CGGAGTATTC TGTGTACACA ATTATCCACG AGGCAACTCT TACACCCGGT 
TCTGAATTTA AGACCCACAG TGGGTTAGTT TGCAAGAAGT CAAAGAGTGC AGAAAATAAC 
TTGCCTAGCT CTTTTTGTTC TTCACCTTTC TCTTTGCTGA ATGCAAATCC CCAAAGAAAA 
TGTGAAACAA GCTGGGAAGC CCCCGGCTCT GCGGGTTCTT TTGCTTCCAG TAGCCTCATC 
TTTGAGGAGA TGCTTGTGGA CCCTCCCATG GGCGAGAATT CGGAAAGCAG CAGTCTTGTA 
GAGTTAGGTG GGGATTCCCA AGACCTCTTG GTAACTGCGT GCGCGTACAA AAGGGAAGAT 
GTGAGCCAAT TGGCTTCCCC TTTAAAACTG AGGCAGCAGC AGGACAGCAG GTACTGTGGT 
GAGAGGACCT TTGGAAACTG TGCAAGTGCT GGGCTCTGTG CTGCTGCCCT GGACTGCTCG 
GATAACTTTA GGACTGCGGG AAGCAAGCTC CCTTTTCCAG CCTGTGAAAA TGTTGGTTTT 
CTACATCATC GTCACACAGA TGAGCAGAAA GATTTGATGT GTACCTCCAG GAGGTCCCAA 
CTCGATGTGC ACTCACCAGT GCTCAGAAGC GCGTTGGCTT TCCCACCCTC CCCAGCCATC 
AAACAGACCA GCCCTTGCAA GTCCAAGAGC CTTGGTGACT TGACATCGGA AGATATTTCC 
TGCAATTTTG AAAGTAAGTA TCAGTACATA AGTAGGAGCT TTGTCACATC TGGCATGAGA 
GACAAGAAGC TCGCTGCCAT GAAGACGATG AGACCGCACT CTACGGATGC TTTGACGGAA 
CAGCTGAGAA AGCTGCTGTC CCTGGACCAG GACGACAGCC AGCAGATGCT GTATTCCAGG 
CGGCTCGACG AGGACTGCCC AAAGGCACTG GTCAGAAAGC TGTCGTCCAG GAGCCAGAGC 
AGAGTGCGGA ACATCGCCAG CCGTGCCAAG GAGAGGCAGG AGGCCAGCAA GCAAAAATCT 
TCCACCGCAG GCAGCATAGC GGGGGTCGTC CTTCGGAACA AGCCTTCGGC GTCCCCTCAC 
GTCGTCAACA GGCACTCGAC GGGCTCCTAC ATAGCCAGGT ACCTGGGCAG CTTCCCTGAG 
GAGGCAGCAG AGGGCCGGGG CCGACCTGAG GGCACGTGTG CTGCCTGGCA CCGCGGCTGT 
GCTGAGCGGC TGTGTGCGCA CAGCCCCCTG CTGCAGCTGG AGCCCGACGG CGACGAGAAG 
CCTGAAGTCT ATTTCCTGCT GAGGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025009536.1
CDS	353..5482
Translation	MSYWSLDKKSCLQYCRHFLADNGVFHVERCMSVMQSGTQMVKLKSGTKGLVRLFYLDEHRTCIRWRPSRKSEKAKIVIDSIYKVTEGRQSEIFHRHAEGSFDPSCCFTIYHGNHLESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDHWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLNFEEFSVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTPEYCLDIIQKFEVSEENKEQNVLGIEGFTNFMRSPACDVFNPLHCEVHQDMDQPLCNYFIASSHNTYLTGDQLLSQSRVEMYARVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKIFFRDVAETINKYAFIKNEFPVILSIENHCSIQQQKKIAQYLKEILGDKLDLSSVVTGDSRQLPSPQNLKGKILVKGKKLPYSLGADAEEGEVSDEDSADEIEDDCKLKSCYSNGATEHQVESFIRTKLESLLKESQIRDKEDPDSFTVRALLRATHEGLSVNLKQNLDTKEGGKKSHSRSLMGNFGKHKKTGKATSKCSTSDDDENQQNSSGKEAGQLHSRLTRRRKTVKLCRALSDLVVYTNSVAAQDIVDDGSTGNVLSFSETRAHQAVQQKAEQFMLYNQKQLTRVYPSAYRIDSSNFNPLPYWNVGCQLVALNYQSEGRMMQLNEAKFRVNGNCGYVLKPQQMCKGSFNPYSSDPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTIHMPEIALVRFLVWDHDPIGRDFVGQRTLAFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSKQLIGLRGLFNKNPKHSSAESSGHYVRKRSIGDRILRRTASAPAKGRKKSKMGFLEASEIKDSASEPADLKDKEGVVRRTSRSLQARPASMPVDKFLLVGLPCTEDETAQDAKGKDSATANSDGDKTEKETNLKESLFTCSEKTADHSNLASPLKKESAQKETTADFSLVPQEQPEHSVAVSDVTETNRPISNQESLLPAETVPLIPDSAFERITEKIQGKSCAGSRRADDTNRCEEKITPVRTCLPEKVEEVENLKYDIIHRSTVACLSVTDSSPAVYSDAPGLCSSSTMQDSDISRLIDEVSLVTESEMDSAVSALIGQLDVTDDQTSLTAPMLPGAGSRAFSAVATRTLTAELSTPCKKDFIATQSSKSPLFSTPDSAVFPSPETAEYSVYTIIHEATLTPGSEFKTHSGLVCKKSKSAENNLPSSFCSSPFSLLNANPQRKCETSWEAPGSAGSFASSSLIFEEMLVDPPMGENSESSSLVELGGDSQDLLVTACAYKREDVSQLASPLKLRQQQDSRYCGERTFGNCASAGLCAAALDCSDNFRTAGSKLPFPACENVGFLHHRHTDEQKDLMCTSRRSQLDVHSPVLRSALAFPPSPAIKQTSPCKSKSLGDLTSEDISCNFESKYQYISRSFVTSGMRDKKLAAMKTMRPHSTDALTEQLRKLLSLDQDDSQQMLYSRRLDEDCPKALVRKLSSRSQSRVRNIASRAKERQEASKQKSSTAGSIAGVVLRNKPSASPHVVNRHSTGSYIARYLGSFPEEAAEGRGRPEGTCAAWHRGCAERLCAHSPLLQLEPDGDEKPEVYFLLRL

Target ORF information:

RefSeq Version	XM_025153768.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025153768.1

ORF Insert Sequence:

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGTAGA 
CTGACTCGTC GAAGGAAAAC TGTGAAGCTG TGCCGAGCTC TGTCTGACCT AGTGGTGTAC 
ACGAACTCTG TGGCAGCCCA GGATATAGTA GATGATGGAT CAACAGGGAA TGTGCTGTCA 
TTCAGTGAAA CAAGAGCCCA CCAAGCAGTG CAGCAGAAGG CTGAACAGTT CATGCTTTAC 
AACCAGAAAC AGCTTACAAG GGTTTATCCA TCAGCGTATC GGATTGACTC CAGCAATTTC 
AATCCTTTAC CTTACTGGAA TGTTGGTTGC CAGCTAGTGG CACTAAACTA CCAGTCTGAG 
GGACGTATGA TGCAATTAAA CGAAGCAAAA TTTAGGGTAA ATGGCAACTG TGGTTACGTC 
CTCAAACCTC AGCAGATGTG CAAAGGCTCA TTCAACCCCT ATTCTTCAGA TCCACTTCCT 
GCTAGTCCTA AGAAGCAGCT CATCCTGAAA ATCATCAGTG GACAGCAGCT GCCTAAGCCT 
CCTGACTCAA TGCTAGGAGA CAGAGGAGAG ATAATAGATC CCTTTGTCGA GGTGGAAATT 
ATTGGGTTAC CTGTTGACTG CTGTAAAGAT CAGACCAGGG TGGTTGATGA CAATGGGTTT 
AATCCTGTTT GGGAGGAAAC GCTCACTTTT ACCATCCATA TGCCTGAAAT AGCTTTGGTT 
CGATTTCTTG TTTGGGATCA TGACCCCATT GGGCGAGACT TCGTTGGACA AAGAACGCTG 
GCCTTTAGCA GTCTTGTACC TGGTTATCGA CATGTGTATT TAGAAGGGCT GACAGAAGCT 
TCCATATTTG TTCACATAAC CATTAATGAA ATCTATGGGA AGTGGAGCCC CTTAATCCTC 
AATCCCAGTT ACACAATATT GCACTTTCTA GGAGCCACAA AGAGTAAGCA ACTGATCGGA 
CTCCGAGGGC TGTTTAACAA GAACCCAAAG CACAGTTCTG CTGAAAGCAG CGGTCATTAT 
GTACGGAAGC GTTCTATTGG TGACCGAATT CTCCGGCGCA CGGCCAGTGC TCCAGCAAAA 
GGTAGAAAGA AAAGCAAGAT GGGTTTTCTG GAAGCTTCTG AAATTAAAGA CAGTGCATCC 
GAACCAGCAG ACCTGAAGGA CAAAGAAGGA GTTGTAAGGC GTACAAGCCG GAGTTTGCAA 
GCACGTCCTG CTTCTATGCC AGTGGACAAA TTCCTTCTTG TAGGACTGCC GTGCACAGAA 
GATGAAACTG CTCAAGATGC CAAAGGAAAA GACAGTGCAA CTGCCAACAG TGATGGCGAT 
AAAACTGAGA AGGAAACAAA CTTGAAGGAA TCTCTTTTCA CGTGTTCTGA GAAAACTGCA 
GATCATTCAA ACTTGGCATC TCCCTTGAAA AAGGAGAGCG CCCAGAAGGA AACTACAGCT 
GATTTTTCTC TTGTACCCCA GGAGCAGCCC GAGCATTCGG TTGCTGTAAG TGATGTAACC 
GAAACAAACC GCCCCATAAG CAATCAGGAA AGTCTCCTTC CTGCTGAAAC GGTGCCTCTG 
ATTCCGGACT CTGCCTTTGA GCGCATCACA GAAAAAATAC AGGGCAAGAG CTGTGCAGGC 
AGTAGAAGGG CAGATGACAC AAACAGGTGT GAGGAGAAAA TAACTCCTGT CAGGACTTGC 
CTTCCTGAAA AAGTAGAGGA GGTTGAAAAT CTCAAATACG ACATCATCCA CAGAAGCACT 
GTGGCGTGTC TTTCTGTGAC AGATAGTTCT CCTGCCGTCT ACTCTGATGC TCCTGGTTTA 
TGCTCCTCTT CAACCATGCA AGACAGCGAC ATCTCCCGCC TCATAGACGA AGTTTCTTTG 
GTGACTGAGA GCGAGATGGA CAGCGCTGTC TCAGCTCTGA TCGGCCAGCT GGATGTCACC 
GATGACCAAA CCAGCCTCAC CGCACCGATG CTCCCTGGCG CTGGCAGCCG GGCCTTCAGT 
GCTGTGGCCA CACGCACACT GACTGCAGAG CTCAGCACTC CCTGTAAGAA GGACTTCATT 
GCCACACAGT CCAGCAAGTC CCCGTTATTT TCAACTCCAG ACTCTGCCGT GTTCCCCAGC 
CCCGAGACTG CGGAGTATTC TGTGTACACA ATTATCCACG AGGCAACTCT TACACCCGGT 
TCTGAATTTA AGACCCACAG TGGGTTAGTT TGCAAGAAGT CAAAGAGTGC AGAAAATAAC 
TTGCCTAGCT CTTTTTGTTC TTCACCTTTC TCTTTGCTGA ATGCAAATCC CCAAAGAAAA 
TGTGAAACAA GCTGGGAAGC CCCCGGCTCT GCGGGTTCTT TTGCTTCCAG TAGCCTCATC 
TTTGAGGAGA TGCTTGTGGA CCCTCCCATG GGCGAGAATT CGGAAAGCAG CAGTCTTGTA 
GAGTTAGGTG GGGATTCCCA AGACCTCTTG GTAACTGCGT GCGCGTACAA AAGGGAAGAT 
GTGAGCCAAT TGGCTTCCCC TTTAAAACTG AGGCAGCAGC AGGACAGCAG GTACTGTGGT 
GAGAGGACCT TTGGAAACTG TGCAAGTGCT GGGCTCTGTG CTGCTGCCCT GGACTGCTCG 
GATAACTTTA GGACTGCGGG AAGCAAGCTC CCTTTTCCAG CCTGTGAAAA TGTTGGTTTT 
CTACATCATC GTCACACAGA TGAGCAGAAA GATTTGATGT GTACCTCCAG GAGGTCCCAA 
CTCGATGTGC ACTCACCAGT GCTCAGAAGC GCGTTGGCTT TCCCACCCTC CCCAGCCATC 
AAACAGACCA GCCCTTGCAA GTCCAAGAGC CTTGGTGACT TGACATCGGA AGATATTTCC 
TGCAATTTTG AAAGTAAGTA TCAGTACATA AGTAGGAGCT TTGTCACATC TGGCATGAGA 
GACAAGAAGC TCGCTGCCAT GAAGACGATG AGACCGCACT CTACGGATGC TTTGACGGAA 
CAGCTGAGAA AGCTGCTGTC CCTGGACCAG GACGACAGCC AGCAGATGCT GTATTCCAGG 
CGGCTCGACG AGGACTGCCC AAAGGCACTG GTCAGAAAGC TGTCGTCCAG GAGCCAGAGC 
AGAGTGCGGA ACATCGCCAG CCGTGCCAAG GAGAGGCAGG AGGCCAGCAA GCAAAAATCT 
TCCACCGCAG GCAGCATAGC GGGGGTCGTC CTTCGGAACA AGCCTTCGGC GTCCCCTCAC 
GTCGTCAACA GGCACTCGAC GGGCTCCTAC ATAGCCAGGT ACCTGGGCAG CTTCCCTGAG 
GAGGCAGCAG AGGGCCGGGG CCGACCTGAG GGCACGTGTG CTGCCTGGCA CCGCGGCTGT 
GCTGAGCGGC TGTGTGCGCA CAGCCCCCTG CTGCAGCTGG AGCCCGACGG CGACGAGAAG 
CCTGAAGTCT ATTTCCTGCT GAGGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_015291816.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147302.1 Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa40157
Clone ID Related Accession (Same CDS sequence)	XM_015291816.2 , XM_025153768.1 , XM_015291816.1 , XM_015291817.1 , XM_015291817.2 , XM_025153769.1
Accession Version	XM_015291816.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGTAGA 
CTGACTCGTC GAAGGAAAAC TGTGAAGCTG TGCCGAGCTC TGTCTGACCT AGTGGTGTAC 
ACGAACTCTG TGGCAGCCCA GGATATAGTA GATGATGGAT CAACAGGGAA TGTGCTGTCA 
TTCAGTGAAA CAAGAGCCCA CCAAGCAGTG CAGCAGAAGG CTGAACAGTT CATGCTTTAC 
AACCAGAAAC AGCTTACAAG GGTTTATCCA TCAGCGTATC GGATTGACTC CAGCAATTTC 
AATCCTTTAC CTTACTGGAA TGTTGGTTGC CAGCTAGTGG CACTAAACTA CCAGTCTGAG 
GGACGTATGA TGCAATTAAA CGAAGCAAAA TTTAGGGTAA ATGGCAACTG TGGTTACGTC 
CTCAAACCTC AGCAGATGTG CAAAGGCTCA TTCAACCCCT ATTCTTCAGA TCCACTTCCT 
GCTAGTCCTA AGAAGCAGCT CATCCTGAAA ATCATCAGTG GACAGCAGCT GCCTAAGCCT 
CCTGACTCAA TGCTAGGAGA CAGAGGAGAG ATAATAGATC CCTTTGTCGA GGTGGAAATT 
ATTGGGTTAC CTGTTGACTG CTGTAAAGAT CAGACCAGGG TGGTTGATGA CAATGGGTTT 
AATCCTGTTT GGGAGGAAAC GCTCACTTTT ACCATCCATA TGCCTGAAAT AGCTTTGGTT 
CGATTTCTTG TTTGGGATCA TGACCCCATT GGGCGAGACT TCGTTGGACA AAGAACGCTG 
GCCTTTAGCA GTCTTGTACC TGGTTATCGA CATGTGTATT TAGAAGGGCT GACAGAAGCT 
TCCATATTTG TTCACATAAC CATTAATGAA ATCTATGGGA AGTGGAGCCC CTTAATCCTC 
AATCCCAGTT ACACAATATT GCACTTTCTA GGAGCCACAA AGAGTAAGCA ACTGATCGGA 
CTCCGAGGGC TGTTTAACAA GAACCCAAAG CACAGTTCTG CTGAAAGCAG CGGTCATTAT 
GTACGGAAGC GTTCTATTGG TGACCGAATT CTCCGGCGCA CGGCCAGTGC TCCAGCAAAA 
GGTAGAAAGA AAAGCAAGAT GGGTTTTCTG GAAGCTTCTG AAATTAAAGA CAGTGCATCC 
GAACCAGCAG ACCTGAAGGA CAAAGAAGGA GTTGTAAGGC GTACAAGCCG GAGTTTGCAA 
GCACGTCCTG CTTCTATGCC AGTGGACAAA TTCCTTCTTG TAGGACTGCC GTGCACAGAA 
GATGAAACTG CTCAAGATGC CAAAGGAAAA GACAGTGCAA CTGCCAACAG TGATGGCGAT 
AAAACTGAGA AGGAAACAAA CTTGAAGGAA TCTCTTTTCA CGTGTTCTGA GAAAACTGCA 
GATCATTCAA ACTTGGCATC TCCCTTGAAA AAGGAGAGCG CCCAGAAGGA AACTACAGCT 
GATTTTTCTC TTGTACCCCA GGAGCAGCCC GAGCATTCGG TTGCTGTAAG TGATGTAACC 
GAAACAAACC GCCCCATAAG CAATCAGGAA AGTCTCCTTC CTGCTGAAAC GGTGCCTCTG 
ATTCCGGACT CTGCCTTTGA GCGCATCACA GAAAAAATAC AGGGCAAGAG CTGTGCAGGC 
AGTAGAAGGG CAGATGACAC AAACAGGTGT GAGGAGAAAA TAACTCCTGT CAGGACTTGC 
CTTCCTGAAA AAGTAGAGGA GGTTGAAAAT CTCAAATACG ACATCATCCA CAGAAGCACT 
GTGGCGTGTC TTTCTGTGAC AGATAGTTCT CCTGCCGTCT ACTCTGATGC TCCTGGTTTA 
TGCTCCTCTT CAACCATGCA AGACAGCGAC ATCTCCCGCC TCATAGACGA AGTTTCTTTG 
GTGACTGAGA GCGAGATGGA CAGCGCTGTC TCAGCTCTGA TCGGCCAGCT GGATGTCACC 
GATGACCAAA CCAGCCTCAC CGCACCGATG CTCCCTGGCG CTGGCAGCCG GGCCTTCAGT 
GCTGTGGCCA CACGCACACT GACTGCAGAG CTCAGCACTC CCTGTAAGAA GGACTTCATT 
GCCACACAGT CCAGCAAGTC CCCGTTATTT TCAACTCCAG ACTCTGCCGT GTTCCCCAGC 
CCCGAGACTG CGGAGTATTC TGTGTACACA ATTATCCACG AGGCAACTCT TACACCCGGT 
TCTGAATTTA AGACCCACAG TGGGTTAGTT TGCAAGAAGT CAAAGAGTGC AGAAAATAAC 
TTGCCTAGCT CTTTTTGTTC TTCACCTTTC TCTTTGCTGA ATGCAAATCC CCAAAGAAAA 
TGTGAAACAA GCTGGGAAGC CCCCGGCTCT GCGGGTTCTT TTGCTTCCAG TAGCCTCATC 
TTTGAGGAGA TGCTTGTGGA CCCTCCCATG GGCGAGAATT CGGAAAGCAG CAGTCTTGTA 
GAGTTAGGTG GGGATTCCCA AGACCTCTTG GTAACTGCGT GCGCGTACAA AAGGGAAGAT 
GTGAGCCAAT TGGCTTCCCC TTTAAAACTG AGGCAGCAGC AGGACAGCAG GTACTGTGGT 
GAGAGGACCT TTGGAAACTG TGCAAGTGCT GGGCTCTGTG CTGCTGCCCT GGACTGCTCG 
GATAACTTTA GGACTGCGGG AAGCAAGCTC CCTTTTCCAG CCTGTGAAAA TGTTGGTTTT 
CTACATCATC GTCACACAGA TGAGCAGAAA GATTTGATGT GTACCTCCAG GAGGTCCCAA 
CTCGATGTGC ACTCACCAGT GCTCAGAAGC GCGTTGGCTT TCCCACCCTC CCCAGCCATC 
AAACAGACCA GCCCTTGCAA GTCCAAGAGC CTTGGTGACT TGACATCGGA AGATATTTCC 
TGCAATTTTG AAAGTAAGTA TCAGTACATA AGTAGGAGCT TTGTCACATC TGGCATGAGA 
GACAAGAAGC TCGCTGCCAT GAAGACGATG AGACCGCACT CTACGGATGC TTTGACGGAA 
CAGCTGAGAA AGCTGCTGTC CCTGGACCAG GACGACAGCC AGCAGATGCT GTATTCCAGG 
CGGCTCGACG AGGACTGCCC AAAGGCACTG GTCAGAAAGC TGTCGTCCAG GAGCCAGAGC 
AGAGTGCGGA ACATCGCCAG CCGTGCCAAG GAGAGGCAGG AGGCCAGCAA GCAAAAATCT 
TCCACCGCAG GCAGCATAGC GGGGGTCGTC CTTCGGAACA AGCCTTCGGC GTCCCCTCAC 
GTCGTCAACA GGCACTCGAC GGGCTCCTAC ATAGCCAGGT ACCTGGGCAG CTTCCCTGAG 
GAGGCAGCAG AGGGCCGGGG CCGACCTGAG GGCACGTGTG CTGCCTGGCA CCGCGGCTGT 
GCTGAGCGGC TGTGTGCGCA CAGCCCCCTG CTGCAGCTGG AGCCCGACGG CGACGAGAAG 
CCTGAAGTCT ATTTCCTGCT GAGGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147302.1
CDS	1381..6510
Translation	MSYWSLDKKSCLQYCRHFLADNGVFHVERCMSVMQSGTQMVKLKSGTKGLVRLFYLDEHRTCIRWRPSRKSEKAKIVIDSIYKVTEGRQSEIFHRHAEGSFDPSCCFTIYHGNHLESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDHWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLNFEEFSVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTPEYCLDIIQKFEVSEENKEQNVLGIEGFTNFMRSPACDVFNPLHCEVHQDMDQPLCNYFIASSHNTYLTGDQLLSQSRVEMYARVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKIFFRDVAETINKYAFIKNEFPVILSIENHCSIQQQKKIAQYLKEILGDKLDLSSVVTGDSRQLPSPQNLKGKILVKGKKLPYSLGADAEEGEVSDEDSADEIEDDCKLKSCYSNGATEHQVESFIRTKLESLLKESQIRDKEDPDSFTVRALLRATHEGLSVNLKQNLDTKEGGKKSHSRSLMGNFGKHKKTGKATSKCSTSDDDENQQNSSGKEAGQLHSRLTRRRKTVKLCRALSDLVVYTNSVAAQDIVDDGSTGNVLSFSETRAHQAVQQKAEQFMLYNQKQLTRVYPSAYRIDSSNFNPLPYWNVGCQLVALNYQSEGRMMQLNEAKFRVNGNCGYVLKPQQMCKGSFNPYSSDPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTIHMPEIALVRFLVWDHDPIGRDFVGQRTLAFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSKQLIGLRGLFNKNPKHSSAESSGHYVRKRSIGDRILRRTASAPAKGRKKSKMGFLEASEIKDSASEPADLKDKEGVVRRTSRSLQARPASMPVDKFLLVGLPCTEDETAQDAKGKDSATANSDGDKTEKETNLKESLFTCSEKTADHSNLASPLKKESAQKETTADFSLVPQEQPEHSVAVSDVTETNRPISNQESLLPAETVPLIPDSAFERITEKIQGKSCAGSRRADDTNRCEEKITPVRTCLPEKVEEVENLKYDIIHRSTVACLSVTDSSPAVYSDAPGLCSSSTMQDSDISRLIDEVSLVTESEMDSAVSALIGQLDVTDDQTSLTAPMLPGAGSRAFSAVATRTLTAELSTPCKKDFIATQSSKSPLFSTPDSAVFPSPETAEYSVYTIIHEATLTPGSEFKTHSGLVCKKSKSAENNLPSSFCSSPFSLLNANPQRKCETSWEAPGSAGSFASSSLIFEEMLVDPPMGENSESSSLVELGGDSQDLLVTACAYKREDVSQLASPLKLRQQQDSRYCGERTFGNCASAGLCAAALDCSDNFRTAGSKLPFPACENVGFLHHRHTDEQKDLMCTSRRSQLDVHSPVLRSALAFPPSPAIKQTSPCKSKSLGDLTSEDISCNFESKYQYISRSFVTSGMRDKKLAAMKTMRPHSTDALTEQLRKLLSLDQDDSQQMLYSRRLDEDCPKALVRKLSSRSQSRVRNIASRAKERQEASKQKSSTAGSIAGVVLRNKPSASPHVVNRHSTGSYIARYLGSFPEEAAEGRGRPEGTCAAWHRGCAERLCAHSPLLQLEPDGDEKPEVYFLLRL

Target ORF information:

RefSeq Version	XM_015291816.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291816.1

ORF Insert Sequence:

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGTAGA 
CTGACTCGTC GAAGGAAAAC TGTGAAGCTG TGCCGAGCTC TGTCTGACCT AGTGGTGTAC 
ACGAACTCTG TGGCAGCCCA GGATATAGTA GATGATGGAT CAACAGGGAA TGTGCTGTCA 
TTCAGTGAAA CAAGAGCCCA CCAAGCAGTG CAGCAGAAGG CTGAACAGTT CATGCTTTAC 
AACCAGAAAC AGCTTACAAG GGTTTATCCA TCAGCGTATC GGATTGACTC CAGCAATTTC 
AATCCTTTAC CTTACTGGAA TGTTGGTTGC CAGCTAGTGG CACTAAACTA CCAGTCTGAG 
GGACGTATGA TGCAATTAAA CGAAGCAAAA TTTAGGGTAA ATGGCAACTG TGGTTACGTC 
CTCAAACCTC AGCAGATGTG CAAAGGCTCA TTCAACCCCT ATTCTTCAGA TCCACTTCCT 
GCTAGTCCTA AGAAGCAGCT CATCCTGAAA ATCATCAGTG GACAGCAGCT GCCTAAGCCT 
CCTGACTCAA TGCTAGGAGA CAGAGGAGAG ATAATAGATC CCTTTGTCGA GGTGGAAATT 
ATTGGGTTAC CTGTTGACTG CTGTAAAGAT CAGACCAGGG TGGTTGATGA CAATGGGTTT 
AATCCTGTTT GGGAGGAAAC GCTCACTTTT ACCATCCATA TGCCTGAAAT AGCTTTGGTT 
CGATTTCTTG TTTGGGATCA TGACCCCATT GGGCGAGACT TCGTTGGACA AAGAACGCTG 
GCCTTTAGCA GTCTTGTACC TGGTTATCGA CATGTGTATT TAGAAGGGCT GACAGAAGCT 
TCCATATTTG TTCACATAAC CATTAATGAA ATCTATGGGA AGTGGAGCCC CTTAATCCTC 
AATCCCAGTT ACACAATATT GCACTTTCTA GGAGCCACAA AGAGTAAGCA ACTGATCGGA 
CTCCGAGGGC TGTTTAACAA GAACCCAAAG CACAGTTCTG CTGAAAGCAG CGGTCATTAT 
GTACGGAAGC GTTCTATTGG TGACCGAATT CTCCGGCGCA CGGCCAGTGC TCCAGCAAAA 
GGTAGAAAGA AAAGCAAGAT GGGTTTTCTG GAAGCTTCTG AAATTAAAGA CAGTGCATCC 
GAACCAGCAG ACCTGAAGGA CAAAGAAGGA GTTGTAAGGC GTACAAGCCG GAGTTTGCAA 
GCACGTCCTG CTTCTATGCC AGTGGACAAA TTCCTTCTTG TAGGACTGCC GTGCACAGAA 
GATGAAACTG CTCAAGATGC CAAAGGAAAA GACAGTGCAA CTGCCAACAG TGATGGCGAT 
AAAACTGAGA AGGAAACAAA CTTGAAGGAA TCTCTTTTCA CGTGTTCTGA GAAAACTGCA 
GATCATTCAA ACTTGGCATC TCCCTTGAAA AAGGAGAGCG CCCAGAAGGA AACTACAGCT 
GATTTTTCTC TTGTACCCCA GGAGCAGCCC GAGCATTCGG TTGCTGTAAG TGATGTAACC 
GAAACAAACC GCCCCATAAG CAATCAGGAA AGTCTCCTTC CTGCTGAAAC GGTGCCTCTG 
ATTCCGGACT CTGCCTTTGA GCGCATCACA GAAAAAATAC AGGGCAAGAG CTGTGCAGGC 
AGTAGAAGGG CAGATGACAC AAACAGGTGT GAGGAGAAAA TAACTCCTGT CAGGACTTGC 
CTTCCTGAAA AAGTAGAGGA GGTTGAAAAT CTCAAATACG ACATCATCCA CAGAAGCACT 
GTGGCGTGTC TTTCTGTGAC AGATAGTTCT CCTGCCGTCT ACTCTGATGC TCCTGGTTTA 
TGCTCCTCTT CAACCATGCA AGACAGCGAC ATCTCCCGCC TCATAGACGA AGTTTCTTTG 
GTGACTGAGA GCGAGATGGA CAGCGCTGTC TCAGCTCTGA TCGGCCAGCT GGATGTCACC 
GATGACCAAA CCAGCCTCAC CGCACCGATG CTCCCTGGCG CTGGCAGCCG GGCCTTCAGT 
GCTGTGGCCA CACGCACACT GACTGCAGAG CTCAGCACTC CCTGTAAGAA GGACTTCATT 
GCCACACAGT CCAGCAAGTC CCCGTTATTT TCAACTCCAG ACTCTGCCGT GTTCCCCAGC 
CCCGAGACTG CGGAGTATTC TGTGTACACA ATTATCCACG AGGCAACTCT TACACCCGGT 
TCTGAATTTA AGACCCACAG TGGGTTAGTT TGCAAGAAGT CAAAGAGTGC AGAAAATAAC 
TTGCCTAGCT CTTTTTGTTC TTCACCTTTC TCTTTGCTGA ATGCAAATCC CCAAAGAAAA 
TGTGAAACAA GCTGGGAAGC CCCCGGCTCT GCGGGTTCTT TTGCTTCCAG TAGCCTCATC 
TTTGAGGAGA TGCTTGTGGA CCCTCCCATG GGCGAGAATT CGGAAAGCAG CAGTCTTGTA 
GAGTTAGGTG GGGATTCCCA AGACCTCTTG GTAACTGCGT GCGCGTACAA AAGGGAAGAT 
GTGAGCCAAT TGGCTTCCCC TTTAAAACTG AGGCAGCAGC AGGACAGCAG GTACTGTGGT 
GAGAGGACCT TTGGAAACTG TGCAAGTGCT GGGCTCTGTG CTGCTGCCCT GGACTGCTCG 
GATAACTTTA GGACTGCGGG AAGCAAGCTC CCTTTTCCAG CCTGTGAAAA TGTTGGTTTT 
CTACATCATC GTCACACAGA TGAGCAGAAA GATTTGATGT GTACCTCCAG GAGGTCCCAA 
CTCGATGTGC ACTCACCAGT GCTCAGAAGC GCGTTGGCTT TCCCACCCTC CCCAGCCATC 
AAACAGACCA GCCCTTGCAA GTCCAAGAGC CTTGGTGACT TGACATCGGA AGATATTTCC 
TGCAATTTTG AAAGTAAGTA TCAGTACATA AGTAGGAGCT TTGTCACATC TGGCATGAGA 
GACAAGAAGC TCGCTGCCAT GAAGACGATG AGACCGCACT CTACGGATGC TTTGACGGAA 
CAGCTGAGAA AGCTGCTGTC CCTGGACCAG GACGACAGCC AGCAGATGCT GTATTCCAGG 
CGGCTCGACG AGGACTGCCC AAAGGCACTG GTCAGAAAGC TGTCGTCCAG GAGCCAGAGC 
AGAGTGCGGA ACATCGCCAG CCGTGCCAAG GAGAGGCAGG AGGCCAGCAA GCAAAAATCT 
TCCACCGCAG GCAGCATAGC GGGGGTCGTC CTTCGGAACA AGCCTTCGGC GTCCCCTCAC 
GTCGTCAACA GGCACTCGAC GGGCTCCTAC ATAGCCAGGT ACCTGGGCAG CTTCCCTGAG 
GAGGCAGCAG AGGGCCGGGG CCGACCTGAG GGCACGTGTG CTGCCTGGCA CCGCGGCTGT 
GCTGAGCGGC TGTGTGCGCA CAGCCCCCTG CTGCAGCTGG AGCCCGACGG CGACGAGAAG 
CCTGAAGTCT ATTTCCTGCT GAGGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_015291817.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147303.1 Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X2, mRNA.

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CloneID	OGa40157
Clone ID Related Accession (Same CDS sequence)	XM_015291816.2 , XM_025153768.1 , XM_015291816.1 , XM_015291817.1 , XM_015291817.2 , XM_025153769.1
Accession Version	XM_015291817.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGTAGA 
CTGACTCGTC GAAGGAAAAC TGTGAAGCTG TGCCGAGCTC TGTCTGACCT AGTGGTGTAC 
ACGAACTCTG TGGCAGCCCA GGATATAGTA GATGATGGAT CAACAGGGAA TGTGCTGTCA 
TTCAGTGAAA CAAGAGCCCA CCAAGCAGTG CAGCAGAAGG CTGAACAGTT CATGCTTTAC 
AACCAGAAAC AGCTTACAAG GGTTTATCCA TCAGCGTATC GGATTGACTC CAGCAATTTC 
AATCCTTTAC CTTACTGGAA TGTTGGTTGC CAGCTAGTGG CACTAAACTA CCAGTCTGAG 
GGACGTATGA TGCAATTAAA CGAAGCAAAA TTTAGGGTAA ATGGCAACTG TGGTTACGTC 
CTCAAACCTC AGCAGATGTG CAAAGGCTCA TTCAACCCCT ATTCTTCAGA TCCACTTCCT 
GCTAGTCCTA AGAAGCAGCT CATCCTGAAA ATCATCAGTG GACAGCAGCT GCCTAAGCCT 
CCTGACTCAA TGCTAGGAGA CAGAGGAGAG ATAATAGATC CCTTTGTCGA GGTGGAAATT 
ATTGGGTTAC CTGTTGACTG CTGTAAAGAT CAGACCAGGG TGGTTGATGA CAATGGGTTT 
AATCCTGTTT GGGAGGAAAC GCTCACTTTT ACCATCCATA TGCCTGAAAT AGCTTTGGTT 
CGATTTCTTG TTTGGGATCA TGACCCCATT GGGCGAGACT TCGTTGGACA AAGAACGCTG 
GCCTTTAGCA GTCTTGTACC TGGTTATCGA CATGTGTATT TAGAAGGGCT GACAGAAGCT 
TCCATATTTG TTCACATAAC CATTAATGAA ATCTATGGGA AGTGGAGCCC CTTAATCCTC 
AATCCCAGTT ACACAATATT GCACTTTCTA GGAGCCACAA AGAGTAAGCA ACTGATCGGA 
CTCCGAGGGC TGTTTAACAA GAACCCAAAG CACAGTTCTG CTGAAAGCAG CGGTCATTAT 
GTACGGAAGC GTTCTATTGG TGACCGAATT CTCCGGCGCA CGGCCAGTGC TCCAGCAAAA 
GGTAGAAAGA AAAGCAAGAT GGGTTTTCTG GAAGCTTCTG AAATTAAAGA CAGTGCATCC 
GAACCAGCAG ACCTGAAGGA CAAAGAAGGA GTTGTAAGGC GTACAAGCCG GAGTTTGCAA 
GCACGTCCTG CTTCTATGCC AGTGGACAAA TTCCTTCTTG TAGGACTGCC GTGCACAGAA 
GATGAAACTG CTCAAGATGC CAAAGGAAAA GACAGTGCAA CTGCCAACAG TGATGGCGAT 
AAAACTGAGA AGGAAACAAA CTTGAAGGAA TCTCTTTTCA CGTGTTCTGA GAAAACTGCA 
GATCATTCAA ACTTGGCATC TCCCTTGAAA AAGGAGAGCG CCCAGAAGGA AACTACAGCT 
GATTTTTCTC TTGTACCCCA GGAGCAGCCC GAGCATTCGG TTGCTGTAAG TGATGTAACC 
GAAACAAACC GCCCCATAAG CAATCAGGAA AGTCTCCTTC CTGCTGAAAC GGTGCCTCTG 
ATTCCGGACT CTGCCTTTGA GCGCATCACA GAAAAAATAC AGGGCAAGAG CTGTGCAGGC 
AGTAGAAGGG CAGATGACAC AAACAGGTGT GAGGAGAAAA TAACTCCTGT CAGGACTTGC 
CTTCCTGAAA AAGTAGAGGA GGTTGAAAAT CTCAAATACG ACATCATCCA CAGAAGCACT 
GTGGCGTGTC TTTCTGTGAC AGATAGTTCT CCTGCCGTCT ACTCTGATGC TCCTGGTTTA 
TGCTCCTCTT CAACCATGCA AGACAGCGAC ATCTCCCGCC TCATAGACGA AGTTTCTTTG 
GTGACTGAGA GCGAGATGGA CAGCGCTGTC TCAGCTCTGA TCGGCCAGCT GGATGTCACC 
GATGACCAAA CCAGCCTCAC CGCACCGATG CTCCCTGGCG CTGGCAGCCG GGCCTTCAGT 
GCTGTGGCCA CACGCACACT GACTGCAGAG CTCAGCACTC CCTGTAAGAA GGACTTCATT 
GCCACACAGT CCAGCAAGTC CCCGTTATTT TCAACTCCAG ACTCTGCCGT GTTCCCCAGC 
CCCGAGACTG CGGAGTATTC TGTGTACACA ATTATCCACG AGGCAACTCT TACACCCGGT 
TCTGAATTTA AGACCCACAG TGGGTTAGTT TGCAAGAAGT CAAAGAGTGC AGAAAATAAC 
TTGCCTAGCT CTTTTTGTTC TTCACCTTTC TCTTTGCTGA ATGCAAATCC CCAAAGAAAA 
TGTGAAACAA GCTGGGAAGC CCCCGGCTCT GCGGGTTCTT TTGCTTCCAG TAGCCTCATC 
TTTGAGGAGA TGCTTGTGGA CCCTCCCATG GGCGAGAATT CGGAAAGCAG CAGTCTTGTA 
GAGTTAGGTG GGGATTCCCA AGACCTCTTG GTAACTGCGT GCGCGTACAA AAGGGAAGAT 
GTGAGCCAAT TGGCTTCCCC TTTAAAACTG AGGCAGCAGC AGGACAGCAG GTACTGTGGT 
GAGAGGACCT TTGGAAACTG TGCAAGTGCT GGGCTCTGTG CTGCTGCCCT GGACTGCTCG 
GATAACTTTA GGACTGCGGG AAGCAAGCTC CCTTTTCCAG CCTGTGAAAA TGTTGGTTTT 
CTACATCATC GTCACACAGA TGAGCAGAAA GATTTGATGT GTACCTCCAG GAGGTCCCAA 
CTCGATGTGC ACTCACCAGT GCTCAGAAGC GCGTTGGCTT TCCCACCCTC CCCAGCCATC 
AAACAGACCA GCCCTTGCAA GTCCAAGAGC CTTGGTGACT TGACATCGGA AGATATTTCC 
TGCAATTTTG AAAGTAAGTA TCAGTACATA AGTAGGAGCT TTGTCACATC TGGCATGAGA 
GACAAGAAGC TCGCTGCCAT GAAGACGATG AGACCGCACT CTACGGATGC TTTGACGGAA 
CAGCTGAGAA AGCTGCTGTC CCTGGACCAG GACGACAGCC AGCAGATGCT GTATTCCAGG 
CGGCTCGACG AGGACTGCCC AAAGGCACTG GTCAGAAAGC TGTCGTCCAG GAGCCAGAGC 
AGAGTGCGGA ACATCGCCAG CCGTGCCAAG GAGAGGCAGG AGGCCAGCAA GCAAAAATCT 
TCCACCGCAG GCAGCATAGC GGGGGTCGTC CTTCGGAACA AGCCTTCGGC GTCCCCTCAC 
GTCGTCAACA GGCACTCGAC GGGCTCCTAC ATAGCCAGGT ACCTGGGCAG CTTCCCTGAG 
GAGGCAGCAG AGGGCCGGGG CCGACCTGAG GGCACGTGTG CTGCCTGGCA CCGCGGCTGT 
GCTGAGCGGC TGTGTGCGCA CAGCCCCCTG CTGCAGCTGG AGCCCGACGG CGACGAGAAG 
CCTGAAGTCT ATTTCCTGCT GAGGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147303.1
CDS	222..5351
Translation	MSYWSLDKKSCLQYCRHFLADNGVFHVERCMSVMQSGTQMVKLKSGTKGLVRLFYLDEHRTCIRWRPSRKSEKAKIVIDSIYKVTEGRQSEIFHRHAEGSFDPSCCFTIYHGNHLESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDHWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLNFEEFSVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTPEYCLDIIQKFEVSEENKEQNVLGIEGFTNFMRSPACDVFNPLHCEVHQDMDQPLCNYFIASSHNTYLTGDQLLSQSRVEMYARVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKIFFRDVAETINKYAFIKNEFPVILSIENHCSIQQQKKIAQYLKEILGDKLDLSSVVTGDSRQLPSPQNLKGKILVKGKKLPYSLGADAEEGEVSDEDSADEIEDDCKLKSCYSNGATEHQVESFIRTKLESLLKESQIRDKEDPDSFTVRALLRATHEGLSVNLKQNLDTKEGGKKSHSRSLMGNFGKHKKTGKATSKCSTSDDDENQQNSSGKEAGQLHSRLTRRRKTVKLCRALSDLVVYTNSVAAQDIVDDGSTGNVLSFSETRAHQAVQQKAEQFMLYNQKQLTRVYPSAYRIDSSNFNPLPYWNVGCQLVALNYQSEGRMMQLNEAKFRVNGNCGYVLKPQQMCKGSFNPYSSDPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTIHMPEIALVRFLVWDHDPIGRDFVGQRTLAFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSKQLIGLRGLFNKNPKHSSAESSGHYVRKRSIGDRILRRTASAPAKGRKKSKMGFLEASEIKDSASEPADLKDKEGVVRRTSRSLQARPASMPVDKFLLVGLPCTEDETAQDAKGKDSATANSDGDKTEKETNLKESLFTCSEKTADHSNLASPLKKESAQKETTADFSLVPQEQPEHSVAVSDVTETNRPISNQESLLPAETVPLIPDSAFERITEKIQGKSCAGSRRADDTNRCEEKITPVRTCLPEKVEEVENLKYDIIHRSTVACLSVTDSSPAVYSDAPGLCSSSTMQDSDISRLIDEVSLVTESEMDSAVSALIGQLDVTDDQTSLTAPMLPGAGSRAFSAVATRTLTAELSTPCKKDFIATQSSKSPLFSTPDSAVFPSPETAEYSVYTIIHEATLTPGSEFKTHSGLVCKKSKSAENNLPSSFCSSPFSLLNANPQRKCETSWEAPGSAGSFASSSLIFEEMLVDPPMGENSESSSLVELGGDSQDLLVTACAYKREDVSQLASPLKLRQQQDSRYCGERTFGNCASAGLCAAALDCSDNFRTAGSKLPFPACENVGFLHHRHTDEQKDLMCTSRRSQLDVHSPVLRSALAFPPSPAIKQTSPCKSKSLGDLTSEDISCNFESKYQYISRSFVTSGMRDKKLAAMKTMRPHSTDALTEQLRKLLSLDQDDSQQMLYSRRLDEDCPKALVRKLSSRSQSRVRNIASRAKERQEASKQKSSTAGSIAGVVLRNKPSASPHVVNRHSTGSYIARYLGSFPEEAAEGRGRPEGTCAAWHRGCAERLCAHSPLLQLEPDGDEKPEVYFLLRL

Target ORF information:

RefSeq Version	XM_015291817.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291817.1

ORF Insert Sequence:

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGTAGA 
CTGACTCGTC GAAGGAAAAC TGTGAAGCTG TGCCGAGCTC TGTCTGACCT AGTGGTGTAC 
ACGAACTCTG TGGCAGCCCA GGATATAGTA GATGATGGAT CAACAGGGAA TGTGCTGTCA 
TTCAGTGAAA CAAGAGCCCA CCAAGCAGTG CAGCAGAAGG CTGAACAGTT CATGCTTTAC 
AACCAGAAAC AGCTTACAAG GGTTTATCCA TCAGCGTATC GGATTGACTC CAGCAATTTC 
AATCCTTTAC CTTACTGGAA TGTTGGTTGC CAGCTAGTGG CACTAAACTA CCAGTCTGAG 
GGACGTATGA TGCAATTAAA CGAAGCAAAA TTTAGGGTAA ATGGCAACTG TGGTTACGTC 
CTCAAACCTC AGCAGATGTG CAAAGGCTCA TTCAACCCCT ATTCTTCAGA TCCACTTCCT 
GCTAGTCCTA AGAAGCAGCT CATCCTGAAA ATCATCAGTG GACAGCAGCT GCCTAAGCCT 
CCTGACTCAA TGCTAGGAGA CAGAGGAGAG ATAATAGATC CCTTTGTCGA GGTGGAAATT 
ATTGGGTTAC CTGTTGACTG CTGTAAAGAT CAGACCAGGG TGGTTGATGA CAATGGGTTT 
AATCCTGTTT GGGAGGAAAC GCTCACTTTT ACCATCCATA TGCCTGAAAT AGCTTTGGTT 
CGATTTCTTG TTTGGGATCA TGACCCCATT GGGCGAGACT TCGTTGGACA AAGAACGCTG 
GCCTTTAGCA GTCTTGTACC TGGTTATCGA CATGTGTATT TAGAAGGGCT GACAGAAGCT 
TCCATATTTG TTCACATAAC CATTAATGAA ATCTATGGGA AGTGGAGCCC CTTAATCCTC 
AATCCCAGTT ACACAATATT GCACTTTCTA GGAGCCACAA AGAGTAAGCA ACTGATCGGA 
CTCCGAGGGC TGTTTAACAA GAACCCAAAG CACAGTTCTG CTGAAAGCAG CGGTCATTAT 
GTACGGAAGC GTTCTATTGG TGACCGAATT CTCCGGCGCA CGGCCAGTGC TCCAGCAAAA 
GGTAGAAAGA AAAGCAAGAT GGGTTTTCTG GAAGCTTCTG AAATTAAAGA CAGTGCATCC 
GAACCAGCAG ACCTGAAGGA CAAAGAAGGA GTTGTAAGGC GTACAAGCCG GAGTTTGCAA 
GCACGTCCTG CTTCTATGCC AGTGGACAAA TTCCTTCTTG TAGGACTGCC GTGCACAGAA 
GATGAAACTG CTCAAGATGC CAAAGGAAAA GACAGTGCAA CTGCCAACAG TGATGGCGAT 
AAAACTGAGA AGGAAACAAA CTTGAAGGAA TCTCTTTTCA CGTGTTCTGA GAAAACTGCA 
GATCATTCAA ACTTGGCATC TCCCTTGAAA AAGGAGAGCG CCCAGAAGGA AACTACAGCT 
GATTTTTCTC TTGTACCCCA GGAGCAGCCC GAGCATTCGG TTGCTGTAAG TGATGTAACC 
GAAACAAACC GCCCCATAAG CAATCAGGAA AGTCTCCTTC CTGCTGAAAC GGTGCCTCTG 
ATTCCGGACT CTGCCTTTGA GCGCATCACA GAAAAAATAC AGGGCAAGAG CTGTGCAGGC 
AGTAGAAGGG CAGATGACAC AAACAGGTGT GAGGAGAAAA TAACTCCTGT CAGGACTTGC 
CTTCCTGAAA AAGTAGAGGA GGTTGAAAAT CTCAAATACG ACATCATCCA CAGAAGCACT 
GTGGCGTGTC TTTCTGTGAC AGATAGTTCT CCTGCCGTCT ACTCTGATGC TCCTGGTTTA 
TGCTCCTCTT CAACCATGCA AGACAGCGAC ATCTCCCGCC TCATAGACGA AGTTTCTTTG 
GTGACTGAGA GCGAGATGGA CAGCGCTGTC TCAGCTCTGA TCGGCCAGCT GGATGTCACC 
GATGACCAAA CCAGCCTCAC CGCACCGATG CTCCCTGGCG CTGGCAGCCG GGCCTTCAGT 
GCTGTGGCCA CACGCACACT GACTGCAGAG CTCAGCACTC CCTGTAAGAA GGACTTCATT 
GCCACACAGT CCAGCAAGTC CCCGTTATTT TCAACTCCAG ACTCTGCCGT GTTCCCCAGC 
CCCGAGACTG CGGAGTATTC TGTGTACACA ATTATCCACG AGGCAACTCT TACACCCGGT 
TCTGAATTTA AGACCCACAG TGGGTTAGTT TGCAAGAAGT CAAAGAGTGC AGAAAATAAC 
TTGCCTAGCT CTTTTTGTTC TTCACCTTTC TCTTTGCTGA ATGCAAATCC CCAAAGAAAA 
TGTGAAACAA GCTGGGAAGC CCCCGGCTCT GCGGGTTCTT TTGCTTCCAG TAGCCTCATC 
TTTGAGGAGA TGCTTGTGGA CCCTCCCATG GGCGAGAATT CGGAAAGCAG CAGTCTTGTA 
GAGTTAGGTG GGGATTCCCA AGACCTCTTG GTAACTGCGT GCGCGTACAA AAGGGAAGAT 
GTGAGCCAAT TGGCTTCCCC TTTAAAACTG AGGCAGCAGC AGGACAGCAG GTACTGTGGT 
GAGAGGACCT TTGGAAACTG TGCAAGTGCT GGGCTCTGTG CTGCTGCCCT GGACTGCTCG 
GATAACTTTA GGACTGCGGG AAGCAAGCTC CCTTTTCCAG CCTGTGAAAA TGTTGGTTTT 
CTACATCATC GTCACACAGA TGAGCAGAAA GATTTGATGT GTACCTCCAG GAGGTCCCAA 
CTCGATGTGC ACTCACCAGT GCTCAGAAGC GCGTTGGCTT TCCCACCCTC CCCAGCCATC 
AAACAGACCA GCCCTTGCAA GTCCAAGAGC CTTGGTGACT TGACATCGGA AGATATTTCC 
TGCAATTTTG AAAGTAAGTA TCAGTACATA AGTAGGAGCT TTGTCACATC TGGCATGAGA 
GACAAGAAGC TCGCTGCCAT GAAGACGATG AGACCGCACT CTACGGATGC TTTGACGGAA 
CAGCTGAGAA AGCTGCTGTC CCTGGACCAG GACGACAGCC AGCAGATGCT GTATTCCAGG 
CGGCTCGACG AGGACTGCCC AAAGGCACTG GTCAGAAAGC TGTCGTCCAG GAGCCAGAGC 
AGAGTGCGGA ACATCGCCAG CCGTGCCAAG GAGAGGCAGG AGGCCAGCAA GCAAAAATCT 
TCCACCGCAG GCAGCATAGC GGGGGTCGTC CTTCGGAACA AGCCTTCGGC GTCCCCTCAC 
GTCGTCAACA GGCACTCGAC GGGCTCCTAC ATAGCCAGGT ACCTGGGCAG CTTCCCTGAG 
GAGGCAGCAG AGGGCCGGGG CCGACCTGAG GGCACGTGTG CTGCCTGGCA CCGCGGCTGT 
GCTGAGCGGC TGTGTGCGCA CAGCCCCCTG CTGCAGCTGG AGCCCGACGG CGACGAGAAG 
CCTGAAGTCT ATTTCCTGCT GAGGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_015291817.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147303.1 Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X1, mRNA.

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CloneID	OGa40157
Clone ID Related Accession (Same CDS sequence)	XM_015291816.2 , XM_025153768.1 , XM_015291816.1 , XM_015291817.1 , XM_015291817.2 , XM_025153769.1
Accession Version	XM_015291817.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291817.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGTAGA 
CTGACTCGTC GAAGGAAAAC TGTGAAGCTG TGCCGAGCTC TGTCTGACCT AGTGGTGTAC 
ACGAACTCTG TGGCAGCCCA GGATATAGTA GATGATGGAT CAACAGGGAA TGTGCTGTCA 
TTCAGTGAAA CAAGAGCCCA CCAAGCAGTG CAGCAGAAGG CTGAACAGTT CATGCTTTAC 
AACCAGAAAC AGCTTACAAG GGTTTATCCA TCAGCGTATC GGATTGACTC CAGCAATTTC 
AATCCTTTAC CTTACTGGAA TGTTGGTTGC CAGCTAGTGG CACTAAACTA CCAGTCTGAG 
GGACGTATGA TGCAATTAAA CGAAGCAAAA TTTAGGGTAA ATGGCAACTG TGGTTACGTC 
CTCAAACCTC AGCAGATGTG CAAAGGCTCA TTCAACCCCT ATTCTTCAGA TCCACTTCCT 
GCTAGTCCTA AGAAGCAGCT CATCCTGAAA ATCATCAGTG GACAGCAGCT GCCTAAGCCT 
CCTGACTCAA TGCTAGGAGA CAGAGGAGAG ATAATAGATC CCTTTGTCGA GGTGGAAATT 
ATTGGGTTAC CTGTTGACTG CTGTAAAGAT CAGACCAGGG TGGTTGATGA CAATGGGTTT 
AATCCTGTTT GGGAGGAAAC GCTCACTTTT ACCATCCATA TGCCTGAAAT AGCTTTGGTT 
CGATTTCTTG TTTGGGATCA TGACCCCATT GGGCGAGACT TCGTTGGACA AAGAACGCTG 
GCCTTTAGCA GTCTTGTACC TGGTTATCGA CATGTGTATT TAGAAGGGCT GACAGAAGCT 
TCCATATTTG TTCACATAAC CATTAATGAA ATCTATGGGA AGTGGAGCCC CTTAATCCTC 
AATCCCAGTT ACACAATATT GCACTTTCTA GGAGCCACAA AGAGTAAGCA ACTGATCGGA 
CTCCGAGGGC TGTTTAACAA GAACCCAAAG CACAGTTCTG CTGAAAGCAG CGGTCATTAT 
GTACGGAAGC GTTCTATTGG TGACCGAATT CTCCGGCGCA CGGCCAGTGC TCCAGCAAAA 
GGTAGAAAGA AAAGCAAGAT GGGTTTTCTG GAAGCTTCTG AAATTAAAGA CAGTGCATCC 
GAACCAGCAG ACCTGAAGGA CAAAGAAGGA GTTGTAAGGC GTACAAGCCG GAGTTTGCAA 
GCACGTCCTG CTTCTATGCC AGTGGACAAA TTCCTTCTTG TAGGACTGCC GTGCACAGAA 
GATGAAACTG CTCAAGATGC CAAAGGAAAA GACAGTGCAA CTGCCAACAG TGATGGCGAT 
AAAACTGAGA AGGAAACAAA CTTGAAGGAA TCTCTTTTCA CGTGTTCTGA GAAAACTGCA 
GATCATTCAA ACTTGGCATC TCCCTTGAAA AAGGAGAGCG CCCAGAAGGA AACTACAGCT 
GATTTTTCTC TTGTACCCCA GGAGCAGCCC GAGCATTCGG TTGCTGTAAG TGATGTAACC 
GAAACAAACC GCCCCATAAG CAATCAGGAA AGTCTCCTTC CTGCTGAAAC GGTGCCTCTG 
ATTCCGGACT CTGCCTTTGA GCGCATCACA GAAAAAATAC AGGGCAAGAG CTGTGCAGGC 
AGTAGAAGGG CAGATGACAC AAACAGGTGT GAGGAGAAAA TAACTCCTGT CAGGACTTGC 
CTTCCTGAAA AAGTAGAGGA GGTTGAAAAT CTCAAATACG ACATCATCCA CAGAAGCACT 
GTGGCGTGTC TTTCTGTGAC AGATAGTTCT CCTGCCGTCT ACTCTGATGC TCCTGGTTTA 
TGCTCCTCTT CAACCATGCA AGACAGCGAC ATCTCCCGCC TCATAGACGA AGTTTCTTTG 
GTGACTGAGA GCGAGATGGA CAGCGCTGTC TCAGCTCTGA TCGGCCAGCT GGATGTCACC 
GATGACCAAA CCAGCCTCAC CGCACCGATG CTCCCTGGCG CTGGCAGCCG GGCCTTCAGT 
GCTGTGGCCA CACGCACACT GACTGCAGAG CTCAGCACTC CCTGTAAGAA GGACTTCATT 
GCCACACAGT CCAGCAAGTC CCCGTTATTT TCAACTCCAG ACTCTGCCGT GTTCCCCAGC 
CCCGAGACTG CGGAGTATTC TGTGTACACA ATTATCCACG AGGCAACTCT TACACCCGGT 
TCTGAATTTA AGACCCACAG TGGGTTAGTT TGCAAGAAGT CAAAGAGTGC AGAAAATAAC 
TTGCCTAGCT CTTTTTGTTC TTCACCTTTC TCTTTGCTGA ATGCAAATCC CCAAAGAAAA 
TGTGAAACAA GCTGGGAAGC CCCCGGCTCT GCGGGTTCTT TTGCTTCCAG TAGCCTCATC 
TTTGAGGAGA TGCTTGTGGA CCCTCCCATG GGCGAGAATT CGGAAAGCAG CAGTCTTGTA 
GAGTTAGGTG GGGATTCCCA AGACCTCTTG GTAACTGCGT GCGCGTACAA AAGGGAAGAT 
GTGAGCCAAT TGGCTTCCCC TTTAAAACTG AGGCAGCAGC AGGACAGCAG GTACTGTGGT 
GAGAGGACCT TTGGAAACTG TGCAAGTGCT GGGCTCTGTG CTGCTGCCCT GGACTGCTCG 
GATAACTTTA GGACTGCGGG AAGCAAGCTC CCTTTTCCAG CCTGTGAAAA TGTTGGTTTT 
CTACATCATC GTCACACAGA TGAGCAGAAA GATTTGATGT GTACCTCCAG GAGGTCCCAA 
CTCGATGTGC ACTCACCAGT GCTCAGAAGC GCGTTGGCTT TCCCACCCTC CCCAGCCATC 
AAACAGACCA GCCCTTGCAA GTCCAAGAGC CTTGGTGACT TGACATCGGA AGATATTTCC 
TGCAATTTTG AAAGTAAGTA TCAGTACATA AGTAGGAGCT TTGTCACATC TGGCATGAGA 
GACAAGAAGC TCGCTGCCAT GAAGACGATG AGACCGCACT CTACGGATGC TTTGACGGAA 
CAGCTGAGAA AGCTGCTGTC CCTGGACCAG GACGACAGCC AGCAGATGCT GTATTCCAGG 
CGGCTCGACG AGGACTGCCC AAAGGCACTG GTCAGAAAGC TGTCGTCCAG GAGCCAGAGC 
AGAGTGCGGA ACATCGCCAG CCGTGCCAAG GAGAGGCAGG AGGCCAGCAA GCAAAAATCT 
TCCACCGCAG GCAGCATAGC GGGGGTCGTC CTTCGGAACA AGCCTTCGGC GTCCCCTCAC 
GTCGTCAACA GGCACTCGAC GGGCTCCTAC ATAGCCAGGT ACCTGGGCAG CTTCCCTGAG 
GAGGCAGCAG AGGGCCGGGG CCGACCTGAG GGCACGTGTG CTGCCTGGCA CCGCGGCTGT 
GCTGAGCGGC TGTGTGCGCA CAGCCCCCTG CTGCAGCTGG AGCCCGACGG CGACGAGAAG 
CCTGAAGTCT ATTTCCTGCT GAGGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147303.1
CDS	338..5467
Translation	MSYWSLDKKSCLQYCRHFLADNGVFHVERCMSVMQSGTQMVKLKSGTKGLVRLFYLDEHRTCIRWRPSRKSEKAKIVIDSIYKVTEGRQSEIFHRHAEGSFDPSCCFTIYHGNHLESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDHWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLNFEEFSVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTPEYCLDIIQKFEVSEENKEQNVLGIEGFTNFMRSPACDVFNPLHCEVHQDMDQPLCNYFIASSHNTYLTGDQLLSQSRVEMYARVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKIFFRDVAETINKYAFIKNEFPVILSIENHCSIQQQKKIAQYLKEILGDKLDLSSVVTGDSRQLPSPQNLKGKILVKGKKLPYSLGADAEEGEVSDEDSADEIEDDCKLKSCYSNGATEHQVESFIRTKLESLLKESQIRDKEDPDSFTVRALLRATHEGLSVNLKQNLDTKEGGKKSHSRSLMGNFGKHKKTGKATSKCSTSDDDENQQNSSGKEAGQLHSRLTRRRKTVKLCRALSDLVVYTNSVAAQDIVDDGSTGNVLSFSETRAHQAVQQKAEQFMLYNQKQLTRVYPSAYRIDSSNFNPLPYWNVGCQLVALNYQSEGRMMQLNEAKFRVNGNCGYVLKPQQMCKGSFNPYSSDPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTIHMPEIALVRFLVWDHDPIGRDFVGQRTLAFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSKQLIGLRGLFNKNPKHSSAESSGHYVRKRSIGDRILRRTASAPAKGRKKSKMGFLEASEIKDSASEPADLKDKEGVVRRTSRSLQARPASMPVDKFLLVGLPCTEDETAQDAKGKDSATANSDGDKTEKETNLKESLFTCSEKTADHSNLASPLKKESAQKETTADFSLVPQEQPEHSVAVSDVTETNRPISNQESLLPAETVPLIPDSAFERITEKIQGKSCAGSRRADDTNRCEEKITPVRTCLPEKVEEVENLKYDIIHRSTVACLSVTDSSPAVYSDAPGLCSSSTMQDSDISRLIDEVSLVTESEMDSAVSALIGQLDVTDDQTSLTAPMLPGAGSRAFSAVATRTLTAELSTPCKKDFIATQSSKSPLFSTPDSAVFPSPETAEYSVYTIIHEATLTPGSEFKTHSGLVCKKSKSAENNLPSSFCSSPFSLLNANPQRKCETSWEAPGSAGSFASSSLIFEEMLVDPPMGENSESSSLVELGGDSQDLLVTACAYKREDVSQLASPLKLRQQQDSRYCGERTFGNCASAGLCAAALDCSDNFRTAGSKLPFPACENVGFLHHRHTDEQKDLMCTSRRSQLDVHSPVLRSALAFPPSPAIKQTSPCKSKSLGDLTSEDISCNFESKYQYISRSFVTSGMRDKKLAAMKTMRPHSTDALTEQLRKLLSLDQDDSQQMLYSRRLDEDCPKALVRKLSSRSQSRVRNIASRAKERQEASKQKSSTAGSIAGVVLRNKPSASPHVVNRHSTGSYIARYLGSFPEEAAEGRGRPEGTCAAWHRGCAERLCAHSPLLQLEPDGDEKPEVYFLLRL

Target ORF information:

RefSeq Version	XM_015291817.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291817.2

ORF Insert Sequence:

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGTAGA 
CTGACTCGTC GAAGGAAAAC TGTGAAGCTG TGCCGAGCTC TGTCTGACCT AGTGGTGTAC 
ACGAACTCTG TGGCAGCCCA GGATATAGTA GATGATGGAT CAACAGGGAA TGTGCTGTCA 
TTCAGTGAAA CAAGAGCCCA CCAAGCAGTG CAGCAGAAGG CTGAACAGTT CATGCTTTAC 
AACCAGAAAC AGCTTACAAG GGTTTATCCA TCAGCGTATC GGATTGACTC CAGCAATTTC 
AATCCTTTAC CTTACTGGAA TGTTGGTTGC CAGCTAGTGG CACTAAACTA CCAGTCTGAG 
GGACGTATGA TGCAATTAAA CGAAGCAAAA TTTAGGGTAA ATGGCAACTG TGGTTACGTC 
CTCAAACCTC AGCAGATGTG CAAAGGCTCA TTCAACCCCT ATTCTTCAGA TCCACTTCCT 
GCTAGTCCTA AGAAGCAGCT CATCCTGAAA ATCATCAGTG GACAGCAGCT GCCTAAGCCT 
CCTGACTCAA TGCTAGGAGA CAGAGGAGAG ATAATAGATC CCTTTGTCGA GGTGGAAATT 
ATTGGGTTAC CTGTTGACTG CTGTAAAGAT CAGACCAGGG TGGTTGATGA CAATGGGTTT 
AATCCTGTTT GGGAGGAAAC GCTCACTTTT ACCATCCATA TGCCTGAAAT AGCTTTGGTT 
CGATTTCTTG TTTGGGATCA TGACCCCATT GGGCGAGACT TCGTTGGACA AAGAACGCTG 
GCCTTTAGCA GTCTTGTACC TGGTTATCGA CATGTGTATT TAGAAGGGCT GACAGAAGCT 
TCCATATTTG TTCACATAAC CATTAATGAA ATCTATGGGA AGTGGAGCCC CTTAATCCTC 
AATCCCAGTT ACACAATATT GCACTTTCTA GGAGCCACAA AGAGTAAGCA ACTGATCGGA 
CTCCGAGGGC TGTTTAACAA GAACCCAAAG CACAGTTCTG CTGAAAGCAG CGGTCATTAT 
GTACGGAAGC GTTCTATTGG TGACCGAATT CTCCGGCGCA CGGCCAGTGC TCCAGCAAAA 
GGTAGAAAGA AAAGCAAGAT GGGTTTTCTG GAAGCTTCTG AAATTAAAGA CAGTGCATCC 
GAACCAGCAG ACCTGAAGGA CAAAGAAGGA GTTGTAAGGC GTACAAGCCG GAGTTTGCAA 
GCACGTCCTG CTTCTATGCC AGTGGACAAA TTCCTTCTTG TAGGACTGCC GTGCACAGAA 
GATGAAACTG CTCAAGATGC CAAAGGAAAA GACAGTGCAA CTGCCAACAG TGATGGCGAT 
AAAACTGAGA AGGAAACAAA CTTGAAGGAA TCTCTTTTCA CGTGTTCTGA GAAAACTGCA 
GATCATTCAA ACTTGGCATC TCCCTTGAAA AAGGAGAGCG CCCAGAAGGA AACTACAGCT 
GATTTTTCTC TTGTACCCCA GGAGCAGCCC GAGCATTCGG TTGCTGTAAG TGATGTAACC 
GAAACAAACC GCCCCATAAG CAATCAGGAA AGTCTCCTTC CTGCTGAAAC GGTGCCTCTG 
ATTCCGGACT CTGCCTTTGA GCGCATCACA GAAAAAATAC AGGGCAAGAG CTGTGCAGGC 
AGTAGAAGGG CAGATGACAC AAACAGGTGT GAGGAGAAAA TAACTCCTGT CAGGACTTGC 
CTTCCTGAAA AAGTAGAGGA GGTTGAAAAT CTCAAATACG ACATCATCCA CAGAAGCACT 
GTGGCGTGTC TTTCTGTGAC AGATAGTTCT CCTGCCGTCT ACTCTGATGC TCCTGGTTTA 
TGCTCCTCTT CAACCATGCA AGACAGCGAC ATCTCCCGCC TCATAGACGA AGTTTCTTTG 
GTGACTGAGA GCGAGATGGA CAGCGCTGTC TCAGCTCTGA TCGGCCAGCT GGATGTCACC 
GATGACCAAA CCAGCCTCAC CGCACCGATG CTCCCTGGCG CTGGCAGCCG GGCCTTCAGT 
GCTGTGGCCA CACGCACACT GACTGCAGAG CTCAGCACTC CCTGTAAGAA GGACTTCATT 
GCCACACAGT CCAGCAAGTC CCCGTTATTT TCAACTCCAG ACTCTGCCGT GTTCCCCAGC 
CCCGAGACTG CGGAGTATTC TGTGTACACA ATTATCCACG AGGCAACTCT TACACCCGGT 
TCTGAATTTA AGACCCACAG TGGGTTAGTT TGCAAGAAGT CAAAGAGTGC AGAAAATAAC 
TTGCCTAGCT CTTTTTGTTC TTCACCTTTC TCTTTGCTGA ATGCAAATCC CCAAAGAAAA 
TGTGAAACAA GCTGGGAAGC CCCCGGCTCT GCGGGTTCTT TTGCTTCCAG TAGCCTCATC 
TTTGAGGAGA TGCTTGTGGA CCCTCCCATG GGCGAGAATT CGGAAAGCAG CAGTCTTGTA 
GAGTTAGGTG GGGATTCCCA AGACCTCTTG GTAACTGCGT GCGCGTACAA AAGGGAAGAT 
GTGAGCCAAT TGGCTTCCCC TTTAAAACTG AGGCAGCAGC AGGACAGCAG GTACTGTGGT 
GAGAGGACCT TTGGAAACTG TGCAAGTGCT GGGCTCTGTG CTGCTGCCCT GGACTGCTCG 
GATAACTTTA GGACTGCGGG AAGCAAGCTC CCTTTTCCAG CCTGTGAAAA TGTTGGTTTT 
CTACATCATC GTCACACAGA TGAGCAGAAA GATTTGATGT GTACCTCCAG GAGGTCCCAA 
CTCGATGTGC ACTCACCAGT GCTCAGAAGC GCGTTGGCTT TCCCACCCTC CCCAGCCATC 
AAACAGACCA GCCCTTGCAA GTCCAAGAGC CTTGGTGACT TGACATCGGA AGATATTTCC 
TGCAATTTTG AAAGTAAGTA TCAGTACATA AGTAGGAGCT TTGTCACATC TGGCATGAGA 
GACAAGAAGC TCGCTGCCAT GAAGACGATG AGACCGCACT CTACGGATGC TTTGACGGAA 
CAGCTGAGAA AGCTGCTGTC CCTGGACCAG GACGACAGCC AGCAGATGCT GTATTCCAGG 
CGGCTCGACG AGGACTGCCC AAAGGCACTG GTCAGAAAGC TGTCGTCCAG GAGCCAGAGC 
AGAGTGCGGA ACATCGCCAG CCGTGCCAAG GAGAGGCAGG AGGCCAGCAA GCAAAAATCT 
TCCACCGCAG GCAGCATAGC GGGGGTCGTC CTTCGGAACA AGCCTTCGGC GTCCCCTCAC 
GTCGTCAACA GGCACTCGAC GGGCTCCTAC ATAGCCAGGT ACCTGGGCAG CTTCCCTGAG 
GAGGCAGCAG AGGGCCGGGG CCGACCTGAG GGCACGTGTG CTGCCTGGCA CCGCGGCTGT 
GCTGAGCGGC TGTGTGCGCA CAGCCCCCTG CTGCAGCTGG AGCCCGACGG CGACGAGAAG 
CCTGAAGTCT ATTTCCTGCT GAGGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_025153769.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025009537.1 Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.

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CloneID	OGa40157
Clone ID Related Accession (Same CDS sequence)	XM_015291816.2 , XM_025153768.1 , XM_015291816.1 , XM_015291817.1 , XM_015291817.2 , XM_025153769.1
Accession Version	XM_025153769.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGTAGA 
CTGACTCGTC GAAGGAAAAC TGTGAAGCTG TGCCGAGCTC TGTCTGACCT AGTGGTGTAC 
ACGAACTCTG TGGCAGCCCA GGATATAGTA GATGATGGAT CAACAGGGAA TGTGCTGTCA 
TTCAGTGAAA CAAGAGCCCA CCAAGCAGTG CAGCAGAAGG CTGAACAGTT CATGCTTTAC 
AACCAGAAAC AGCTTACAAG GGTTTATCCA TCAGCGTATC GGATTGACTC CAGCAATTTC 
AATCCTTTAC CTTACTGGAA TGTTGGTTGC CAGCTAGTGG CACTAAACTA CCAGTCTGAG 
GGACGTATGA TGCAATTAAA CGAAGCAAAA TTTAGGGTAA ATGGCAACTG TGGTTACGTC 
CTCAAACCTC AGCAGATGTG CAAAGGCTCA TTCAACCCCT ATTCTTCAGA TCCACTTCCT 
GCTAGTCCTA AGAAGCAGCT CATCCTGAAA ATCATCAGTG GACAGCAGCT GCCTAAGCCT 
CCTGACTCAA TGCTAGGAGA CAGAGGAGAG ATAATAGATC CCTTTGTCGA GGTGGAAATT 
ATTGGGTTAC CTGTTGACTG CTGTAAAGAT CAGACCAGGG TGGTTGATGA CAATGGGTTT 
AATCCTGTTT GGGAGGAAAC GCTCACTTTT ACCATCCATA TGCCTGAAAT AGCTTTGGTT 
CGATTTCTTG TTTGGGATCA TGACCCCATT GGGCGAGACT TCGTTGGACA AAGAACGCTG 
GCCTTTAGCA GTCTTGTACC TGGTTATCGA CATGTGTATT TAGAAGGGCT GACAGAAGCT 
TCCATATTTG TTCACATAAC CATTAATGAA ATCTATGGGA AGTGGAGCCC CTTAATCCTC 
AATCCCAGTT ACACAATATT GCACTTTCTA GGAGCCACAA AGAGTAAGCA ACTGATCGGA 
CTCCGAGGGC TGTTTAACAA GAACCCAAAG CACAGTTCTG CTGAAAGCAG CGGTCATTAT 
GTACGGAAGC GTTCTATTGG TGACCGAATT CTCCGGCGCA CGGCCAGTGC TCCAGCAAAA 
GGTAGAAAGA AAAGCAAGAT GGGTTTTCTG GAAGCTTCTG AAATTAAAGA CAGTGCATCC 
GAACCAGCAG ACCTGAAGGA CAAAGAAGGA GTTGTAAGGC GTACAAGCCG GAGTTTGCAA 
GCACGTCCTG CTTCTATGCC AGTGGACAAA TTCCTTCTTG TAGGACTGCC GTGCACAGAA 
GATGAAACTG CTCAAGATGC CAAAGGAAAA GACAGTGCAA CTGCCAACAG TGATGGCGAT 
AAAACTGAGA AGGAAACAAA CTTGAAGGAA TCTCTTTTCA CGTGTTCTGA GAAAACTGCA 
GATCATTCAA ACTTGGCATC TCCCTTGAAA AAGGAGAGCG CCCAGAAGGA AACTACAGCT 
GATTTTTCTC TTGTACCCCA GGAGCAGCCC GAGCATTCGG TTGCTGTAAG TGATGTAACC 
GAAACAAACC GCCCCATAAG CAATCAGGAA AGTCTCCTTC CTGCTGAAAC GGTGCCTCTG 
ATTCCGGACT CTGCCTTTGA GCGCATCACA GAAAAAATAC AGGGCAAGAG CTGTGCAGGC 
AGTAGAAGGG CAGATGACAC AAACAGGTGT GAGGAGAAAA TAACTCCTGT CAGGACTTGC 
CTTCCTGAAA AAGTAGAGGA GGTTGAAAAT CTCAAATACG ACATCATCCA CAGAAGCACT 
GTGGCGTGTC TTTCTGTGAC AGATAGTTCT CCTGCCGTCT ACTCTGATGC TCCTGGTTTA 
TGCTCCTCTT CAACCATGCA AGACAGCGAC ATCTCCCGCC TCATAGACGA AGTTTCTTTG 
GTGACTGAGA GCGAGATGGA CAGCGCTGTC TCAGCTCTGA TCGGCCAGCT GGATGTCACC 
GATGACCAAA CCAGCCTCAC CGCACCGATG CTCCCTGGCG CTGGCAGCCG GGCCTTCAGT 
GCTGTGGCCA CACGCACACT GACTGCAGAG CTCAGCACTC CCTGTAAGAA GGACTTCATT 
GCCACACAGT CCAGCAAGTC CCCGTTATTT TCAACTCCAG ACTCTGCCGT GTTCCCCAGC 
CCCGAGACTG CGGAGTATTC TGTGTACACA ATTATCCACG AGGCAACTCT TACACCCGGT 
TCTGAATTTA AGACCCACAG TGGGTTAGTT TGCAAGAAGT CAAAGAGTGC AGAAAATAAC 
TTGCCTAGCT CTTTTTGTTC TTCACCTTTC TCTTTGCTGA ATGCAAATCC CCAAAGAAAA 
TGTGAAACAA GCTGGGAAGC CCCCGGCTCT GCGGGTTCTT TTGCTTCCAG TAGCCTCATC 
TTTGAGGAGA TGCTTGTGGA CCCTCCCATG GGCGAGAATT CGGAAAGCAG CAGTCTTGTA 
GAGTTAGGTG GGGATTCCCA AGACCTCTTG GTAACTGCGT GCGCGTACAA AAGGGAAGAT 
GTGAGCCAAT TGGCTTCCCC TTTAAAACTG AGGCAGCAGC AGGACAGCAG GTACTGTGGT 
GAGAGGACCT TTGGAAACTG TGCAAGTGCT GGGCTCTGTG CTGCTGCCCT GGACTGCTCG 
GATAACTTTA GGACTGCGGG AAGCAAGCTC CCTTTTCCAG CCTGTGAAAA TGTTGGTTTT 
CTACATCATC GTCACACAGA TGAGCAGAAA GATTTGATGT GTACCTCCAG GAGGTCCCAA 
CTCGATGTGC ACTCACCAGT GCTCAGAAGC GCGTTGGCTT TCCCACCCTC CCCAGCCATC 
AAACAGACCA GCCCTTGCAA GTCCAAGAGC CTTGGTGACT TGACATCGGA AGATATTTCC 
TGCAATTTTG AAAGTAAGTA TCAGTACATA AGTAGGAGCT TTGTCACATC TGGCATGAGA 
GACAAGAAGC TCGCTGCCAT GAAGACGATG AGACCGCACT CTACGGATGC TTTGACGGAA 
CAGCTGAGAA AGCTGCTGTC CCTGGACCAG GACGACAGCC AGCAGATGCT GTATTCCAGG 
CGGCTCGACG AGGACTGCCC AAAGGCACTG GTCAGAAAGC TGTCGTCCAG GAGCCAGAGC 
AGAGTGCGGA ACATCGCCAG CCGTGCCAAG GAGAGGCAGG AGGCCAGCAA GCAAAAATCT 
TCCACCGCAG GCAGCATAGC GGGGGTCGTC CTTCGGAACA AGCCTTCGGC GTCCCCTCAC 
GTCGTCAACA GGCACTCGAC GGGCTCCTAC ATAGCCAGGT ACCTGGGCAG CTTCCCTGAG 
GAGGCAGCAG AGGGCCGGGG CCGACCTGAG GGCACGTGTG CTGCCTGGCA CCGCGGCTGT 
GCTGAGCGGC TGTGTGCGCA CAGCCCCCTG CTGCAGCTGG AGCCCGACGG CGACGAGAAG 
CCTGAAGTCT ATTTCCTGCT GAGGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025009537.1
CDS	161..5290
Translation	MSYWSLDKKSCLQYCRHFLADNGVFHVERCMSVMQSGTQMVKLKSGTKGLVRLFYLDEHRTCIRWRPSRKSEKAKIVIDSIYKVTEGRQSEIFHRHAEGSFDPSCCFTIYHGNHLESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDHWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLNFEEFSVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTPEYCLDIIQKFEVSEENKEQNVLGIEGFTNFMRSPACDVFNPLHCEVHQDMDQPLCNYFIASSHNTYLTGDQLLSQSRVEMYARVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKIFFRDVAETINKYAFIKNEFPVILSIENHCSIQQQKKIAQYLKEILGDKLDLSSVVTGDSRQLPSPQNLKGKILVKGKKLPYSLGADAEEGEVSDEDSADEIEDDCKLKSCYSNGATEHQVESFIRTKLESLLKESQIRDKEDPDSFTVRALLRATHEGLSVNLKQNLDTKEGGKKSHSRSLMGNFGKHKKTGKATSKCSTSDDDENQQNSSGKEAGQLHSRLTRRRKTVKLCRALSDLVVYTNSVAAQDIVDDGSTGNVLSFSETRAHQAVQQKAEQFMLYNQKQLTRVYPSAYRIDSSNFNPLPYWNVGCQLVALNYQSEGRMMQLNEAKFRVNGNCGYVLKPQQMCKGSFNPYSSDPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTIHMPEIALVRFLVWDHDPIGRDFVGQRTLAFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSKQLIGLRGLFNKNPKHSSAESSGHYVRKRSIGDRILRRTASAPAKGRKKSKMGFLEASEIKDSASEPADLKDKEGVVRRTSRSLQARPASMPVDKFLLVGLPCTEDETAQDAKGKDSATANSDGDKTEKETNLKESLFTCSEKTADHSNLASPLKKESAQKETTADFSLVPQEQPEHSVAVSDVTETNRPISNQESLLPAETVPLIPDSAFERITEKIQGKSCAGSRRADDTNRCEEKITPVRTCLPEKVEEVENLKYDIIHRSTVACLSVTDSSPAVYSDAPGLCSSSTMQDSDISRLIDEVSLVTESEMDSAVSALIGQLDVTDDQTSLTAPMLPGAGSRAFSAVATRTLTAELSTPCKKDFIATQSSKSPLFSTPDSAVFPSPETAEYSVYTIIHEATLTPGSEFKTHSGLVCKKSKSAENNLPSSFCSSPFSLLNANPQRKCETSWEAPGSAGSFASSSLIFEEMLVDPPMGENSESSSLVELGGDSQDLLVTACAYKREDVSQLASPLKLRQQQDSRYCGERTFGNCASAGLCAAALDCSDNFRTAGSKLPFPACENVGFLHHRHTDEQKDLMCTSRRSQLDVHSPVLRSALAFPPSPAIKQTSPCKSKSLGDLTSEDISCNFESKYQYISRSFVTSGMRDKKLAAMKTMRPHSTDALTEQLRKLLSLDQDDSQQMLYSRRLDEDCPKALVRKLSSRSQSRVRNIASRAKERQEASKQKSSTAGSIAGVVLRNKPSASPHVVNRHSTGSYIARYLGSFPEEAAEGRGRPEGTCAAWHRGCAERLCAHSPLLQLEPDGDEKPEVYFLLRL

Target ORF information:

RefSeq Version	XM_025153769.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025153769.1

ORF Insert Sequence:

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGTAGA 
CTGACTCGTC GAAGGAAAAC TGTGAAGCTG TGCCGAGCTC TGTCTGACCT AGTGGTGTAC 
ACGAACTCTG TGGCAGCCCA GGATATAGTA GATGATGGAT CAACAGGGAA TGTGCTGTCA 
TTCAGTGAAA CAAGAGCCCA CCAAGCAGTG CAGCAGAAGG CTGAACAGTT CATGCTTTAC 
AACCAGAAAC AGCTTACAAG GGTTTATCCA TCAGCGTATC GGATTGACTC CAGCAATTTC 
AATCCTTTAC CTTACTGGAA TGTTGGTTGC CAGCTAGTGG CACTAAACTA CCAGTCTGAG 
GGACGTATGA TGCAATTAAA CGAAGCAAAA TTTAGGGTAA ATGGCAACTG TGGTTACGTC 
CTCAAACCTC AGCAGATGTG CAAAGGCTCA TTCAACCCCT ATTCTTCAGA TCCACTTCCT 
GCTAGTCCTA AGAAGCAGCT CATCCTGAAA ATCATCAGTG GACAGCAGCT GCCTAAGCCT 
CCTGACTCAA TGCTAGGAGA CAGAGGAGAG ATAATAGATC CCTTTGTCGA GGTGGAAATT 
ATTGGGTTAC CTGTTGACTG CTGTAAAGAT CAGACCAGGG TGGTTGATGA CAATGGGTTT 
AATCCTGTTT GGGAGGAAAC GCTCACTTTT ACCATCCATA TGCCTGAAAT AGCTTTGGTT 
CGATTTCTTG TTTGGGATCA TGACCCCATT GGGCGAGACT TCGTTGGACA AAGAACGCTG 
GCCTTTAGCA GTCTTGTACC TGGTTATCGA CATGTGTATT TAGAAGGGCT GACAGAAGCT 
TCCATATTTG TTCACATAAC CATTAATGAA ATCTATGGGA AGTGGAGCCC CTTAATCCTC 
AATCCCAGTT ACACAATATT GCACTTTCTA GGAGCCACAA AGAGTAAGCA ACTGATCGGA 
CTCCGAGGGC TGTTTAACAA GAACCCAAAG CACAGTTCTG CTGAAAGCAG CGGTCATTAT 
GTACGGAAGC GTTCTATTGG TGACCGAATT CTCCGGCGCA CGGCCAGTGC TCCAGCAAAA 
GGTAGAAAGA AAAGCAAGAT GGGTTTTCTG GAAGCTTCTG AAATTAAAGA CAGTGCATCC 
GAACCAGCAG ACCTGAAGGA CAAAGAAGGA GTTGTAAGGC GTACAAGCCG GAGTTTGCAA 
GCACGTCCTG CTTCTATGCC AGTGGACAAA TTCCTTCTTG TAGGACTGCC GTGCACAGAA 
GATGAAACTG CTCAAGATGC CAAAGGAAAA GACAGTGCAA CTGCCAACAG TGATGGCGAT 
AAAACTGAGA AGGAAACAAA CTTGAAGGAA TCTCTTTTCA CGTGTTCTGA GAAAACTGCA 
GATCATTCAA ACTTGGCATC TCCCTTGAAA AAGGAGAGCG CCCAGAAGGA AACTACAGCT 
GATTTTTCTC TTGTACCCCA GGAGCAGCCC GAGCATTCGG TTGCTGTAAG TGATGTAACC 
GAAACAAACC GCCCCATAAG CAATCAGGAA AGTCTCCTTC CTGCTGAAAC GGTGCCTCTG 
ATTCCGGACT CTGCCTTTGA GCGCATCACA GAAAAAATAC AGGGCAAGAG CTGTGCAGGC 
AGTAGAAGGG CAGATGACAC AAACAGGTGT GAGGAGAAAA TAACTCCTGT CAGGACTTGC 
CTTCCTGAAA AAGTAGAGGA GGTTGAAAAT CTCAAATACG ACATCATCCA CAGAAGCACT 
GTGGCGTGTC TTTCTGTGAC AGATAGTTCT CCTGCCGTCT ACTCTGATGC TCCTGGTTTA 
TGCTCCTCTT CAACCATGCA AGACAGCGAC ATCTCCCGCC TCATAGACGA AGTTTCTTTG 
GTGACTGAGA GCGAGATGGA CAGCGCTGTC TCAGCTCTGA TCGGCCAGCT GGATGTCACC 
GATGACCAAA CCAGCCTCAC CGCACCGATG CTCCCTGGCG CTGGCAGCCG GGCCTTCAGT 
GCTGTGGCCA CACGCACACT GACTGCAGAG CTCAGCACTC CCTGTAAGAA GGACTTCATT 
GCCACACAGT CCAGCAAGTC CCCGTTATTT TCAACTCCAG ACTCTGCCGT GTTCCCCAGC 
CCCGAGACTG CGGAGTATTC TGTGTACACA ATTATCCACG AGGCAACTCT TACACCCGGT 
TCTGAATTTA AGACCCACAG TGGGTTAGTT TGCAAGAAGT CAAAGAGTGC AGAAAATAAC 
TTGCCTAGCT CTTTTTGTTC TTCACCTTTC TCTTTGCTGA ATGCAAATCC CCAAAGAAAA 
TGTGAAACAA GCTGGGAAGC CCCCGGCTCT GCGGGTTCTT TTGCTTCCAG TAGCCTCATC 
TTTGAGGAGA TGCTTGTGGA CCCTCCCATG GGCGAGAATT CGGAAAGCAG CAGTCTTGTA 
GAGTTAGGTG GGGATTCCCA AGACCTCTTG GTAACTGCGT GCGCGTACAA AAGGGAAGAT 
GTGAGCCAAT TGGCTTCCCC TTTAAAACTG AGGCAGCAGC AGGACAGCAG GTACTGTGGT 
GAGAGGACCT TTGGAAACTG TGCAAGTGCT GGGCTCTGTG CTGCTGCCCT GGACTGCTCG 
GATAACTTTA GGACTGCGGG AAGCAAGCTC CCTTTTCCAG CCTGTGAAAA TGTTGGTTTT 
CTACATCATC GTCACACAGA TGAGCAGAAA GATTTGATGT GTACCTCCAG GAGGTCCCAA 
CTCGATGTGC ACTCACCAGT GCTCAGAAGC GCGTTGGCTT TCCCACCCTC CCCAGCCATC 
AAACAGACCA GCCCTTGCAA GTCCAAGAGC CTTGGTGACT TGACATCGGA AGATATTTCC 
TGCAATTTTG AAAGTAAGTA TCAGTACATA AGTAGGAGCT TTGTCACATC TGGCATGAGA 
GACAAGAAGC TCGCTGCCAT GAAGACGATG AGACCGCACT CTACGGATGC TTTGACGGAA 
CAGCTGAGAA AGCTGCTGTC CCTGGACCAG GACGACAGCC AGCAGATGCT GTATTCCAGG 
CGGCTCGACG AGGACTGCCC AAAGGCACTG GTCAGAAAGC TGTCGTCCAG GAGCCAGAGC 
AGAGTGCGGA ACATCGCCAG CCGTGCCAAG GAGAGGCAGG AGGCCAGCAA GCAAAAATCT 
TCCACCGCAG GCAGCATAGC GGGGGTCGTC CTTCGGAACA AGCCTTCGGC GTCCCCTCAC 
GTCGTCAACA GGCACTCGAC GGGCTCCTAC ATAGCCAGGT ACCTGGGCAG CTTCCCTGAG 
GAGGCAGCAG AGGGCCGGGG CCGACCTGAG GGCACGTGTG CTGCCTGGCA CCGCGGCTGT 
GCTGAGCGGC TGTGTGCGCA CAGCCCCCTG CTGCAGCTGG AGCCCGACGG CGACGAGAAG 
CCTGAAGTCT ATTTCCTGCT GAGGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_015291818.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147304.1 Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X3, mRNA.

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CloneID	OGa40158
Clone ID Related Accession (Same CDS sequence)	XM_015291818.1 , XM_025153770.1
Accession Version	XM_015291818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGACTG 
ACTCGTCGAA GGAAAACTGT GAAGCTGTGC CGAGCTCTGT CTGACCTAGT GGTGTACACG 
AACTCTGTGG CAGCCCAGGA TATAGTAGAT GATGGATCAA CAGGGAATGT GCTGTCATTC 
AGTGAAACAA GAGCCCACCA AGCAGTGCAG CAGAAGGCTG AACAGTTCAT GCTTTACAAC 
CAGAAACAGC TTACAAGGGT TTATCCATCA GCGTATCGGA TTGACTCCAG CAATTTCAAT 
CCTTTACCTT ACTGGAATGT TGGTTGCCAG CTAGTGGCAC TAAACTACCA GTCTGAGGGA 
CGTATGATGC AATTAAACGA AGCAAAATTT AGGGTAAATG GCAACTGTGG TTACGTCCTC 
AAACCTCAGC AGATGTGCAA AGGCTCATTC AACCCCTATT CTTCAGATCC ACTTCCTGCT 
AGTCCTAAGA AGCAGCTCAT CCTGAAAATC ATCAGTGGAC AGCAGCTGCC TAAGCCTCCT 
GACTCAATGC TAGGAGACAG AGGAGAGATA ATAGATCCCT TTGTCGAGGT GGAAATTATT 
GGGTTACCTG TTGACTGCTG TAAAGATCAG ACCAGGGTGG TTGATGACAA TGGGTTTAAT 
CCTGTTTGGG AGGAAACGCT CACTTTTACC ATCCATATGC CTGAAATAGC TTTGGTTCGA 
TTTCTTGTTT GGGATCATGA CCCCATTGGG CGAGACTTCG TTGGACAAAG AACGCTGGCC 
TTTAGCAGTC TTGTACCTGG TTATCGACAT GTGTATTTAG AAGGGCTGAC AGAAGCTTCC 
ATATTTGTTC ACATAACCAT TAATGAAATC TATGGGAAGT GGAGCCCCTT AATCCTCAAT 
CCCAGTTACA CAATATTGCA CTTTCTAGGA GCCACAAAGA GTAAGCAACT GATCGGACTC 
CGAGGGCTGT TTAACAAGAA CCCAAAGCAC AGTTCTGCTG AAAGCAGCGG TCATTATGTA 
CGGAAGCGTT CTATTGGTGA CCGAATTCTC CGGCGCACGG CCAGTGCTCC AGCAAAAGGT 
AGAAAGAAAA GCAAGATGGG TTTTCTGGAA GCTTCTGAAA TTAAAGACAG TGCATCCGAA 
CCAGCAGACC TGAAGGACAA AGAAGGAGTT GTAAGGCGTA CAAGCCGGAG TTTGCAAGCA 
CGTCCTGCTT CTATGCCAGT GGACAAATTC CTTCTTGTAG GACTGCCGTG CACAGAAGAT 
GAAACTGCTC AAGATGCCAA AGGAAAAGAC AGTGCAACTG CCAACAGTGA TGGCGATAAA 
ACTGAGAAGG AAACAAACTT GAAGGAATCT CTTTTCACGT GTTCTGAGAA AACTGCAGAT 
CATTCAAACT TGGCATCTCC CTTGAAAAAG GAGAGCGCCC AGAAGGAAAC TACAGCTGAT 
TTTTCTCTTG TACCCCAGGA GCAGCCCGAG CATTCGGTTG CTGTAAGTGA TGTAACCGAA 
ACAAACCGCC CCATAAGCAA TCAGGAAAGT CTCCTTCCTG CTGAAACGGT GCCTCTGATT 
CCGGACTCTG CCTTTGAGCG CATCACAGAA AAAATACAGG GCAAGAGCTG TGCAGGCAGT 
AGAAGGGCAG ATGACACAAA CAGGTGTGAG GAGAAAATAA CTCCTGTCAG GACTTGCCTT 
CCTGAAAAAG TAGAGGAGGT TGAAAATCTC AAATACGACA TCATCCACAG AAGCACTGTG 
GCGTGTCTTT CTGTGACAGA TAGTTCTCCT GCCGTCTACT CTGATGCTCC TGGTTTATGC 
TCCTCTTCAA CCATGCAAGA CAGCGACATC TCCCGCCTCA TAGACGAAGT TTCTTTGGTG 
ACTGAGAGCG AGATGGACAG CGCTGTCTCA GCTCTGATCG GCCAGCTGGA TGTCACCGAT 
GACCAAACCA GCCTCACCGC ACCGATGCTC CCTGGCGCTG GCAGCCGGGC CTTCAGTGCT 
GTGGCCACAC GCACACTGAC TGCAGAGCTC AGCACTCCCT GTAAGAAGGA CTTCATTGCC 
ACACAGTCCA GCAAGTCCCC GTTATTTTCA ACTCCAGACT CTGCCGTGTT CCCCAGCCCC 
GAGACTGCGG AGTATTCTGT GTACACAATT ATCCACGAGG CAACTCTTAC ACCCGGTTCT 
GAATTTAAGA CCCACAGTGG GTTAGTTTGC AAGAAGTCAA AGAGTGCAGA AAATAACTTG 
CCTAGCTCTT TTTGTTCTTC ACCTTTCTCT TTGCTGAATG CAAATCCCCA AAGAAAATGT 
GAAACAAGCT GGGAAGCCCC CGGCTCTGCG GGTTCTTTTG CTTCCAGTAG CCTCATCTTT 
GAGGAGATGC TTGTGGACCC TCCCATGGGC GAGAATTCGG AAAGCAGCAG TCTTGTAGAG 
TTAGGTGGGG ATTCCCAAGA CCTCTTGGTA ACTGCGTGCG CGTACAAAAG GGAAGATGTG 
AGCCAATTGG CTTCCCCTTT AAAACTGAGG CAGCAGCAGG ACAGCAGGTA CTGTGGTGAG 
AGGACCTTTG GAAACTGTGC AAGTGCTGGG CTCTGTGCTG CTGCCCTGGA CTGCTCGGAT 
AACTTTAGGA CTGCGGGAAG CAAGCTCCCT TTTCCAGCCT GTGAAAATGT TGGTTTTCTA 
CATCATCGTC ACACAGATGA GCAGAAAGAT TTGATGTGTA CCTCCAGGAG GTCCCAACTC 
GATGTGCACT CACCAGTGCT CAGAAGCGCG TTGGCTTTCC CACCCTCCCC AGCCATCAAA 
CAGACCAGCC CTTGCAAGTC CAAGAGCCTT GGTGACTTGA CATCGGAAGA TATTTCCTGC 
AATTTTGAAA GTAAGTATCA GTACATAAGT AGGAGCTTTG TCACATCTGG CATGAGAGAC 
AAGAAGCTCG CTGCCATGAA GACGATGAGA CCGCACTCTA CGGATGCTTT GACGGAACAG 
CTGAGAAAGC TGCTGTCCCT GGACCAGGAC GACAGCCAGC AGATGCTGTA TTCCAGGCGG 
CTCGACGAGG ACTGCCCAAA GGCACTGGTC AGAAAGCTGT CGTCCAGGAG CCAGAGCAGA 
GTGCGGAACA TCGCCAGCCG TGCCAAGGAG AGGCAGGAGG CCAGCAAGCA AAAATCTTCC 
ACCGCAGGCA GCATAGCGGG GGTCGTCCTT CGGAACAAGC CTTCGGCGTC CCCTCACGTC 
GTCAACAGGC ACTCGACGGG CTCCTACATA GCCAGGTACC TGGGCAGCTT CCCTGAGGAG 
GCAGCAGAGG GCCGGGGCCG ACCTGAGGGC ACGTGTGCTG CCTGGCACCG CGGCTGTGCT 
GAGCGGCTGT GTGCGCACAG CCCCCTGCTG CAGCTGGAGC CCGACGGCGA CGAGAAGCCT 
GAAGTCTATT TCCTGCTGAG GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147304.1
CDS	1381..6507
Translation	MSYWSLDKKSCLQYCRHFLADNGVFHVERCMSVMQSGTQMVKLKSGTKGLVRLFYLDEHRTCIRWRPSRKSEKAKIVIDSIYKVTEGRQSEIFHRHAEGSFDPSCCFTIYHGNHLESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDHWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLNFEEFSVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTPEYCLDIIQKFEVSEENKEQNVLGIEGFTNFMRSPACDVFNPLHCEVHQDMDQPLCNYFIASSHNTYLTGDQLLSQSRVEMYARVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKIFFRDVAETINKYAFIKNEFPVILSIENHCSIQQQKKIAQYLKEILGDKLDLSSVVTGDSRQLPSPQNLKGKILVKGKKLPYSLGADAEEGEVSDEDSADEIEDDCKLKSCYSNGATEHQVESFIRTKLESLLKESQIRDKEDPDSFTVRALLRATHEGLSVNLKQNLDTKEGGKKSHSRSLMGNFGKHKKTGKATSKCSTSDDDENQQNSSGKEAGQLHRLTRRRKTVKLCRALSDLVVYTNSVAAQDIVDDGSTGNVLSFSETRAHQAVQQKAEQFMLYNQKQLTRVYPSAYRIDSSNFNPLPYWNVGCQLVALNYQSEGRMMQLNEAKFRVNGNCGYVLKPQQMCKGSFNPYSSDPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTIHMPEIALVRFLVWDHDPIGRDFVGQRTLAFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSKQLIGLRGLFNKNPKHSSAESSGHYVRKRSIGDRILRRTASAPAKGRKKSKMGFLEASEIKDSASEPADLKDKEGVVRRTSRSLQARPASMPVDKFLLVGLPCTEDETAQDAKGKDSATANSDGDKTEKETNLKESLFTCSEKTADHSNLASPLKKESAQKETTADFSLVPQEQPEHSVAVSDVTETNRPISNQESLLPAETVPLIPDSAFERITEKIQGKSCAGSRRADDTNRCEEKITPVRTCLPEKVEEVENLKYDIIHRSTVACLSVTDSSPAVYSDAPGLCSSSTMQDSDISRLIDEVSLVTESEMDSAVSALIGQLDVTDDQTSLTAPMLPGAGSRAFSAVATRTLTAELSTPCKKDFIATQSSKSPLFSTPDSAVFPSPETAEYSVYTIIHEATLTPGSEFKTHSGLVCKKSKSAENNLPSSFCSSPFSLLNANPQRKCETSWEAPGSAGSFASSSLIFEEMLVDPPMGENSESSSLVELGGDSQDLLVTACAYKREDVSQLASPLKLRQQQDSRYCGERTFGNCASAGLCAAALDCSDNFRTAGSKLPFPACENVGFLHHRHTDEQKDLMCTSRRSQLDVHSPVLRSALAFPPSPAIKQTSPCKSKSLGDLTSEDISCNFESKYQYISRSFVTSGMRDKKLAAMKTMRPHSTDALTEQLRKLLSLDQDDSQQMLYSRRLDEDCPKALVRKLSSRSQSRVRNIASRAKERQEASKQKSSTAGSIAGVVLRNKPSASPHVVNRHSTGSYIARYLGSFPEEAAEGRGRPEGTCAAWHRGCAERLCAHSPLLQLEPDGDEKPEVYFLLRL

Target ORF information:

RefSeq Version	XM_015291818.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291818.1

ORF Insert Sequence:

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGACTG 
ACTCGTCGAA GGAAAACTGT GAAGCTGTGC CGAGCTCTGT CTGACCTAGT GGTGTACACG 
AACTCTGTGG CAGCCCAGGA TATAGTAGAT GATGGATCAA CAGGGAATGT GCTGTCATTC 
AGTGAAACAA GAGCCCACCA AGCAGTGCAG CAGAAGGCTG AACAGTTCAT GCTTTACAAC 
CAGAAACAGC TTACAAGGGT TTATCCATCA GCGTATCGGA TTGACTCCAG CAATTTCAAT 
CCTTTACCTT ACTGGAATGT TGGTTGCCAG CTAGTGGCAC TAAACTACCA GTCTGAGGGA 
CGTATGATGC AATTAAACGA AGCAAAATTT AGGGTAAATG GCAACTGTGG TTACGTCCTC 
AAACCTCAGC AGATGTGCAA AGGCTCATTC AACCCCTATT CTTCAGATCC ACTTCCTGCT 
AGTCCTAAGA AGCAGCTCAT CCTGAAAATC ATCAGTGGAC AGCAGCTGCC TAAGCCTCCT 
GACTCAATGC TAGGAGACAG AGGAGAGATA ATAGATCCCT TTGTCGAGGT GGAAATTATT 
GGGTTACCTG TTGACTGCTG TAAAGATCAG ACCAGGGTGG TTGATGACAA TGGGTTTAAT 
CCTGTTTGGG AGGAAACGCT CACTTTTACC ATCCATATGC CTGAAATAGC TTTGGTTCGA 
TTTCTTGTTT GGGATCATGA CCCCATTGGG CGAGACTTCG TTGGACAAAG AACGCTGGCC 
TTTAGCAGTC TTGTACCTGG TTATCGACAT GTGTATTTAG AAGGGCTGAC AGAAGCTTCC 
ATATTTGTTC ACATAACCAT TAATGAAATC TATGGGAAGT GGAGCCCCTT AATCCTCAAT 
CCCAGTTACA CAATATTGCA CTTTCTAGGA GCCACAAAGA GTAAGCAACT GATCGGACTC 
CGAGGGCTGT TTAACAAGAA CCCAAAGCAC AGTTCTGCTG AAAGCAGCGG TCATTATGTA 
CGGAAGCGTT CTATTGGTGA CCGAATTCTC CGGCGCACGG CCAGTGCTCC AGCAAAAGGT 
AGAAAGAAAA GCAAGATGGG TTTTCTGGAA GCTTCTGAAA TTAAAGACAG TGCATCCGAA 
CCAGCAGACC TGAAGGACAA AGAAGGAGTT GTAAGGCGTA CAAGCCGGAG TTTGCAAGCA 
CGTCCTGCTT CTATGCCAGT GGACAAATTC CTTCTTGTAG GACTGCCGTG CACAGAAGAT 
GAAACTGCTC AAGATGCCAA AGGAAAAGAC AGTGCAACTG CCAACAGTGA TGGCGATAAA 
ACTGAGAAGG AAACAAACTT GAAGGAATCT CTTTTCACGT GTTCTGAGAA AACTGCAGAT 
CATTCAAACT TGGCATCTCC CTTGAAAAAG GAGAGCGCCC AGAAGGAAAC TACAGCTGAT 
TTTTCTCTTG TACCCCAGGA GCAGCCCGAG CATTCGGTTG CTGTAAGTGA TGTAACCGAA 
ACAAACCGCC CCATAAGCAA TCAGGAAAGT CTCCTTCCTG CTGAAACGGT GCCTCTGATT 
CCGGACTCTG CCTTTGAGCG CATCACAGAA AAAATACAGG GCAAGAGCTG TGCAGGCAGT 
AGAAGGGCAG ATGACACAAA CAGGTGTGAG GAGAAAATAA CTCCTGTCAG GACTTGCCTT 
CCTGAAAAAG TAGAGGAGGT TGAAAATCTC AAATACGACA TCATCCACAG AAGCACTGTG 
GCGTGTCTTT CTGTGACAGA TAGTTCTCCT GCCGTCTACT CTGATGCTCC TGGTTTATGC 
TCCTCTTCAA CCATGCAAGA CAGCGACATC TCCCGCCTCA TAGACGAAGT TTCTTTGGTG 
ACTGAGAGCG AGATGGACAG CGCTGTCTCA GCTCTGATCG GCCAGCTGGA TGTCACCGAT 
GACCAAACCA GCCTCACCGC ACCGATGCTC CCTGGCGCTG GCAGCCGGGC CTTCAGTGCT 
GTGGCCACAC GCACACTGAC TGCAGAGCTC AGCACTCCCT GTAAGAAGGA CTTCATTGCC 
ACACAGTCCA GCAAGTCCCC GTTATTTTCA ACTCCAGACT CTGCCGTGTT CCCCAGCCCC 
GAGACTGCGG AGTATTCTGT GTACACAATT ATCCACGAGG CAACTCTTAC ACCCGGTTCT 
GAATTTAAGA CCCACAGTGG GTTAGTTTGC AAGAAGTCAA AGAGTGCAGA AAATAACTTG 
CCTAGCTCTT TTTGTTCTTC ACCTTTCTCT TTGCTGAATG CAAATCCCCA AAGAAAATGT 
GAAACAAGCT GGGAAGCCCC CGGCTCTGCG GGTTCTTTTG CTTCCAGTAG CCTCATCTTT 
GAGGAGATGC TTGTGGACCC TCCCATGGGC GAGAATTCGG AAAGCAGCAG TCTTGTAGAG 
TTAGGTGGGG ATTCCCAAGA CCTCTTGGTA ACTGCGTGCG CGTACAAAAG GGAAGATGTG 
AGCCAATTGG CTTCCCCTTT AAAACTGAGG CAGCAGCAGG ACAGCAGGTA CTGTGGTGAG 
AGGACCTTTG GAAACTGTGC AAGTGCTGGG CTCTGTGCTG CTGCCCTGGA CTGCTCGGAT 
AACTTTAGGA CTGCGGGAAG CAAGCTCCCT TTTCCAGCCT GTGAAAATGT TGGTTTTCTA 
CATCATCGTC ACACAGATGA GCAGAAAGAT TTGATGTGTA CCTCCAGGAG GTCCCAACTC 
GATGTGCACT CACCAGTGCT CAGAAGCGCG TTGGCTTTCC CACCCTCCCC AGCCATCAAA 
CAGACCAGCC CTTGCAAGTC CAAGAGCCTT GGTGACTTGA CATCGGAAGA TATTTCCTGC 
AATTTTGAAA GTAAGTATCA GTACATAAGT AGGAGCTTTG TCACATCTGG CATGAGAGAC 
AAGAAGCTCG CTGCCATGAA GACGATGAGA CCGCACTCTA CGGATGCTTT GACGGAACAG 
CTGAGAAAGC TGCTGTCCCT GGACCAGGAC GACAGCCAGC AGATGCTGTA TTCCAGGCGG 
CTCGACGAGG ACTGCCCAAA GGCACTGGTC AGAAAGCTGT CGTCCAGGAG CCAGAGCAGA 
GTGCGGAACA TCGCCAGCCG TGCCAAGGAG AGGCAGGAGG CCAGCAAGCA AAAATCTTCC 
ACCGCAGGCA GCATAGCGGG GGTCGTCCTT CGGAACAAGC CTTCGGCGTC CCCTCACGTC 
GTCAACAGGC ACTCGACGGG CTCCTACATA GCCAGGTACC TGGGCAGCTT CCCTGAGGAG 
GCAGCAGAGG GCCGGGGCCG ACCTGAGGGC ACGTGTGCTG CCTGGCACCG CGGCTGTGCT 
GAGCGGCTGT GTGCGCACAG CCCCCTGCTG CAGCTGGAGC CCGACGGCGA CGAGAAGCCT 
GAAGTCTATT TCCTGCTGAG GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_025153770.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025009538.1 Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X5, mRNA.

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CloneID	OGa40158
Clone ID Related Accession (Same CDS sequence)	XM_015291818.1 , XM_025153770.1
Accession Version	XM_025153770.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGACTG 
ACTCGTCGAA GGAAAACTGT GAAGCTGTGC CGAGCTCTGT CTGACCTAGT GGTGTACACG 
AACTCTGTGG CAGCCCAGGA TATAGTAGAT GATGGATCAA CAGGGAATGT GCTGTCATTC 
AGTGAAACAA GAGCCCACCA AGCAGTGCAG CAGAAGGCTG AACAGTTCAT GCTTTACAAC 
CAGAAACAGC TTACAAGGGT TTATCCATCA GCGTATCGGA TTGACTCCAG CAATTTCAAT 
CCTTTACCTT ACTGGAATGT TGGTTGCCAG CTAGTGGCAC TAAACTACCA GTCTGAGGGA 
CGTATGATGC AATTAAACGA AGCAAAATTT AGGGTAAATG GCAACTGTGG TTACGTCCTC 
AAACCTCAGC AGATGTGCAA AGGCTCATTC AACCCCTATT CTTCAGATCC ACTTCCTGCT 
AGTCCTAAGA AGCAGCTCAT CCTGAAAATC ATCAGTGGAC AGCAGCTGCC TAAGCCTCCT 
GACTCAATGC TAGGAGACAG AGGAGAGATA ATAGATCCCT TTGTCGAGGT GGAAATTATT 
GGGTTACCTG TTGACTGCTG TAAAGATCAG ACCAGGGTGG TTGATGACAA TGGGTTTAAT 
CCTGTTTGGG AGGAAACGCT CACTTTTACC ATCCATATGC CTGAAATAGC TTTGGTTCGA 
TTTCTTGTTT GGGATCATGA CCCCATTGGG CGAGACTTCG TTGGACAAAG AACGCTGGCC 
TTTAGCAGTC TTGTACCTGG TTATCGACAT GTGTATTTAG AAGGGCTGAC AGAAGCTTCC 
ATATTTGTTC ACATAACCAT TAATGAAATC TATGGGAAGT GGAGCCCCTT AATCCTCAAT 
CCCAGTTACA CAATATTGCA CTTTCTAGGA GCCACAAAGA GTAAGCAACT GATCGGACTC 
CGAGGGCTGT TTAACAAGAA CCCAAAGCAC AGTTCTGCTG AAAGCAGCGG TCATTATGTA 
CGGAAGCGTT CTATTGGTGA CCGAATTCTC CGGCGCACGG CCAGTGCTCC AGCAAAAGGT 
AGAAAGAAAA GCAAGATGGG TTTTCTGGAA GCTTCTGAAA TTAAAGACAG TGCATCCGAA 
CCAGCAGACC TGAAGGACAA AGAAGGAGTT GTAAGGCGTA CAAGCCGGAG TTTGCAAGCA 
CGTCCTGCTT CTATGCCAGT GGACAAATTC CTTCTTGTAG GACTGCCGTG CACAGAAGAT 
GAAACTGCTC AAGATGCCAA AGGAAAAGAC AGTGCAACTG CCAACAGTGA TGGCGATAAA 
ACTGAGAAGG AAACAAACTT GAAGGAATCT CTTTTCACGT GTTCTGAGAA AACTGCAGAT 
CATTCAAACT TGGCATCTCC CTTGAAAAAG GAGAGCGCCC AGAAGGAAAC TACAGCTGAT 
TTTTCTCTTG TACCCCAGGA GCAGCCCGAG CATTCGGTTG CTGTAAGTGA TGTAACCGAA 
ACAAACCGCC CCATAAGCAA TCAGGAAAGT CTCCTTCCTG CTGAAACGGT GCCTCTGATT 
CCGGACTCTG CCTTTGAGCG CATCACAGAA AAAATACAGG GCAAGAGCTG TGCAGGCAGT 
AGAAGGGCAG ATGACACAAA CAGGTGTGAG GAGAAAATAA CTCCTGTCAG GACTTGCCTT 
CCTGAAAAAG TAGAGGAGGT TGAAAATCTC AAATACGACA TCATCCACAG AAGCACTGTG 
GCGTGTCTTT CTGTGACAGA TAGTTCTCCT GCCGTCTACT CTGATGCTCC TGGTTTATGC 
TCCTCTTCAA CCATGCAAGA CAGCGACATC TCCCGCCTCA TAGACGAAGT TTCTTTGGTG 
ACTGAGAGCG AGATGGACAG CGCTGTCTCA GCTCTGATCG GCCAGCTGGA TGTCACCGAT 
GACCAAACCA GCCTCACCGC ACCGATGCTC CCTGGCGCTG GCAGCCGGGC CTTCAGTGCT 
GTGGCCACAC GCACACTGAC TGCAGAGCTC AGCACTCCCT GTAAGAAGGA CTTCATTGCC 
ACACAGTCCA GCAAGTCCCC GTTATTTTCA ACTCCAGACT CTGCCGTGTT CCCCAGCCCC 
GAGACTGCGG AGTATTCTGT GTACACAATT ATCCACGAGG CAACTCTTAC ACCCGGTTCT 
GAATTTAAGA CCCACAGTGG GTTAGTTTGC AAGAAGTCAA AGAGTGCAGA AAATAACTTG 
CCTAGCTCTT TTTGTTCTTC ACCTTTCTCT TTGCTGAATG CAAATCCCCA AAGAAAATGT 
GAAACAAGCT GGGAAGCCCC CGGCTCTGCG GGTTCTTTTG CTTCCAGTAG CCTCATCTTT 
GAGGAGATGC TTGTGGACCC TCCCATGGGC GAGAATTCGG AAAGCAGCAG TCTTGTAGAG 
TTAGGTGGGG ATTCCCAAGA CCTCTTGGTA ACTGCGTGCG CGTACAAAAG GGAAGATGTG 
AGCCAATTGG CTTCCCCTTT AAAACTGAGG CAGCAGCAGG ACAGCAGGTA CTGTGGTGAG 
AGGACCTTTG GAAACTGTGC AAGTGCTGGG CTCTGTGCTG CTGCCCTGGA CTGCTCGGAT 
AACTTTAGGA CTGCGGGAAG CAAGCTCCCT TTTCCAGCCT GTGAAAATGT TGGTTTTCTA 
CATCATCGTC ACACAGATGA GCAGAAAGAT TTGATGTGTA CCTCCAGGAG GTCCCAACTC 
GATGTGCACT CACCAGTGCT CAGAAGCGCG TTGGCTTTCC CACCCTCCCC AGCCATCAAA 
CAGACCAGCC CTTGCAAGTC CAAGAGCCTT GGTGACTTGA CATCGGAAGA TATTTCCTGC 
AATTTTGAAA GTAAGTATCA GTACATAAGT AGGAGCTTTG TCACATCTGG CATGAGAGAC 
AAGAAGCTCG CTGCCATGAA GACGATGAGA CCGCACTCTA CGGATGCTTT GACGGAACAG 
CTGAGAAAGC TGCTGTCCCT GGACCAGGAC GACAGCCAGC AGATGCTGTA TTCCAGGCGG 
CTCGACGAGG ACTGCCCAAA GGCACTGGTC AGAAAGCTGT CGTCCAGGAG CCAGAGCAGA 
GTGCGGAACA TCGCCAGCCG TGCCAAGGAG AGGCAGGAGG CCAGCAAGCA AAAATCTTCC 
ACCGCAGGCA GCATAGCGGG GGTCGTCCTT CGGAACAAGC CTTCGGCGTC CCCTCACGTC 
GTCAACAGGC ACTCGACGGG CTCCTACATA GCCAGGTACC TGGGCAGCTT CCCTGAGGAG 
GCAGCAGAGG GCCGGGGCCG ACCTGAGGGC ACGTGTGCTG CCTGGCACCG CGGCTGTGCT 
GAGCGGCTGT GTGCGCACAG CCCCCTGCTG CAGCTGGAGC CCGACGGCGA CGAGAAGCCT 
GAAGTCTATT TCCTGCTGAG GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025009538.1
CDS	338..5464
Translation	MSYWSLDKKSCLQYCRHFLADNGVFHVERCMSVMQSGTQMVKLKSGTKGLVRLFYLDEHRTCIRWRPSRKSEKAKIVIDSIYKVTEGRQSEIFHRHAEGSFDPSCCFTIYHGNHLESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDHWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLNFEEFSVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTPEYCLDIIQKFEVSEENKEQNVLGIEGFTNFMRSPACDVFNPLHCEVHQDMDQPLCNYFIASSHNTYLTGDQLLSQSRVEMYARVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKIFFRDVAETINKYAFIKNEFPVILSIENHCSIQQQKKIAQYLKEILGDKLDLSSVVTGDSRQLPSPQNLKGKILVKGKKLPYSLGADAEEGEVSDEDSADEIEDDCKLKSCYSNGATEHQVESFIRTKLESLLKESQIRDKEDPDSFTVRALLRATHEGLSVNLKQNLDTKEGGKKSHSRSLMGNFGKHKKTGKATSKCSTSDDDENQQNSSGKEAGQLHRLTRRRKTVKLCRALSDLVVYTNSVAAQDIVDDGSTGNVLSFSETRAHQAVQQKAEQFMLYNQKQLTRVYPSAYRIDSSNFNPLPYWNVGCQLVALNYQSEGRMMQLNEAKFRVNGNCGYVLKPQQMCKGSFNPYSSDPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTIHMPEIALVRFLVWDHDPIGRDFVGQRTLAFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSKQLIGLRGLFNKNPKHSSAESSGHYVRKRSIGDRILRRTASAPAKGRKKSKMGFLEASEIKDSASEPADLKDKEGVVRRTSRSLQARPASMPVDKFLLVGLPCTEDETAQDAKGKDSATANSDGDKTEKETNLKESLFTCSEKTADHSNLASPLKKESAQKETTADFSLVPQEQPEHSVAVSDVTETNRPISNQESLLPAETVPLIPDSAFERITEKIQGKSCAGSRRADDTNRCEEKITPVRTCLPEKVEEVENLKYDIIHRSTVACLSVTDSSPAVYSDAPGLCSSSTMQDSDISRLIDEVSLVTESEMDSAVSALIGQLDVTDDQTSLTAPMLPGAGSRAFSAVATRTLTAELSTPCKKDFIATQSSKSPLFSTPDSAVFPSPETAEYSVYTIIHEATLTPGSEFKTHSGLVCKKSKSAENNLPSSFCSSPFSLLNANPQRKCETSWEAPGSAGSFASSSLIFEEMLVDPPMGENSESSSLVELGGDSQDLLVTACAYKREDVSQLASPLKLRQQQDSRYCGERTFGNCASAGLCAAALDCSDNFRTAGSKLPFPACENVGFLHHRHTDEQKDLMCTSRRSQLDVHSPVLRSALAFPPSPAIKQTSPCKSKSLGDLTSEDISCNFESKYQYISRSFVTSGMRDKKLAAMKTMRPHSTDALTEQLRKLLSLDQDDSQQMLYSRRLDEDCPKALVRKLSSRSQSRVRNIASRAKERQEASKQKSSTAGSIAGVVLRNKPSASPHVVNRHSTGSYIARYLGSFPEEAAEGRGRPEGTCAAWHRGCAERLCAHSPLLQLEPDGDEKPEVYFLLRL

Target ORF information:

RefSeq Version	XM_025153770.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025153770.1

ORF Insert Sequence:

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGACTG 
ACTCGTCGAA GGAAAACTGT GAAGCTGTGC CGAGCTCTGT CTGACCTAGT GGTGTACACG 
AACTCTGTGG CAGCCCAGGA TATAGTAGAT GATGGATCAA CAGGGAATGT GCTGTCATTC 
AGTGAAACAA GAGCCCACCA AGCAGTGCAG CAGAAGGCTG AACAGTTCAT GCTTTACAAC 
CAGAAACAGC TTACAAGGGT TTATCCATCA GCGTATCGGA TTGACTCCAG CAATTTCAAT 
CCTTTACCTT ACTGGAATGT TGGTTGCCAG CTAGTGGCAC TAAACTACCA GTCTGAGGGA 
CGTATGATGC AATTAAACGA AGCAAAATTT AGGGTAAATG GCAACTGTGG TTACGTCCTC 
AAACCTCAGC AGATGTGCAA AGGCTCATTC AACCCCTATT CTTCAGATCC ACTTCCTGCT 
AGTCCTAAGA AGCAGCTCAT CCTGAAAATC ATCAGTGGAC AGCAGCTGCC TAAGCCTCCT 
GACTCAATGC TAGGAGACAG AGGAGAGATA ATAGATCCCT TTGTCGAGGT GGAAATTATT 
GGGTTACCTG TTGACTGCTG TAAAGATCAG ACCAGGGTGG TTGATGACAA TGGGTTTAAT 
CCTGTTTGGG AGGAAACGCT CACTTTTACC ATCCATATGC CTGAAATAGC TTTGGTTCGA 
TTTCTTGTTT GGGATCATGA CCCCATTGGG CGAGACTTCG TTGGACAAAG AACGCTGGCC 
TTTAGCAGTC TTGTACCTGG TTATCGACAT GTGTATTTAG AAGGGCTGAC AGAAGCTTCC 
ATATTTGTTC ACATAACCAT TAATGAAATC TATGGGAAGT GGAGCCCCTT AATCCTCAAT 
CCCAGTTACA CAATATTGCA CTTTCTAGGA GCCACAAAGA GTAAGCAACT GATCGGACTC 
CGAGGGCTGT TTAACAAGAA CCCAAAGCAC AGTTCTGCTG AAAGCAGCGG TCATTATGTA 
CGGAAGCGTT CTATTGGTGA CCGAATTCTC CGGCGCACGG CCAGTGCTCC AGCAAAAGGT 
AGAAAGAAAA GCAAGATGGG TTTTCTGGAA GCTTCTGAAA TTAAAGACAG TGCATCCGAA 
CCAGCAGACC TGAAGGACAA AGAAGGAGTT GTAAGGCGTA CAAGCCGGAG TTTGCAAGCA 
CGTCCTGCTT CTATGCCAGT GGACAAATTC CTTCTTGTAG GACTGCCGTG CACAGAAGAT 
GAAACTGCTC AAGATGCCAA AGGAAAAGAC AGTGCAACTG CCAACAGTGA TGGCGATAAA 
ACTGAGAAGG AAACAAACTT GAAGGAATCT CTTTTCACGT GTTCTGAGAA AACTGCAGAT 
CATTCAAACT TGGCATCTCC CTTGAAAAAG GAGAGCGCCC AGAAGGAAAC TACAGCTGAT 
TTTTCTCTTG TACCCCAGGA GCAGCCCGAG CATTCGGTTG CTGTAAGTGA TGTAACCGAA 
ACAAACCGCC CCATAAGCAA TCAGGAAAGT CTCCTTCCTG CTGAAACGGT GCCTCTGATT 
CCGGACTCTG CCTTTGAGCG CATCACAGAA AAAATACAGG GCAAGAGCTG TGCAGGCAGT 
AGAAGGGCAG ATGACACAAA CAGGTGTGAG GAGAAAATAA CTCCTGTCAG GACTTGCCTT 
CCTGAAAAAG TAGAGGAGGT TGAAAATCTC AAATACGACA TCATCCACAG AAGCACTGTG 
GCGTGTCTTT CTGTGACAGA TAGTTCTCCT GCCGTCTACT CTGATGCTCC TGGTTTATGC 
TCCTCTTCAA CCATGCAAGA CAGCGACATC TCCCGCCTCA TAGACGAAGT TTCTTTGGTG 
ACTGAGAGCG AGATGGACAG CGCTGTCTCA GCTCTGATCG GCCAGCTGGA TGTCACCGAT 
GACCAAACCA GCCTCACCGC ACCGATGCTC CCTGGCGCTG GCAGCCGGGC CTTCAGTGCT 
GTGGCCACAC GCACACTGAC TGCAGAGCTC AGCACTCCCT GTAAGAAGGA CTTCATTGCC 
ACACAGTCCA GCAAGTCCCC GTTATTTTCA ACTCCAGACT CTGCCGTGTT CCCCAGCCCC 
GAGACTGCGG AGTATTCTGT GTACACAATT ATCCACGAGG CAACTCTTAC ACCCGGTTCT 
GAATTTAAGA CCCACAGTGG GTTAGTTTGC AAGAAGTCAA AGAGTGCAGA AAATAACTTG 
CCTAGCTCTT TTTGTTCTTC ACCTTTCTCT TTGCTGAATG CAAATCCCCA AAGAAAATGT 
GAAACAAGCT GGGAAGCCCC CGGCTCTGCG GGTTCTTTTG CTTCCAGTAG CCTCATCTTT 
GAGGAGATGC TTGTGGACCC TCCCATGGGC GAGAATTCGG AAAGCAGCAG TCTTGTAGAG 
TTAGGTGGGG ATTCCCAAGA CCTCTTGGTA ACTGCGTGCG CGTACAAAAG GGAAGATGTG 
AGCCAATTGG CTTCCCCTTT AAAACTGAGG CAGCAGCAGG ACAGCAGGTA CTGTGGTGAG 
AGGACCTTTG GAAACTGTGC AAGTGCTGGG CTCTGTGCTG CTGCCCTGGA CTGCTCGGAT 
AACTTTAGGA CTGCGGGAAG CAAGCTCCCT TTTCCAGCCT GTGAAAATGT TGGTTTTCTA 
CATCATCGTC ACACAGATGA GCAGAAAGAT TTGATGTGTA CCTCCAGGAG GTCCCAACTC 
GATGTGCACT CACCAGTGCT CAGAAGCGCG TTGGCTTTCC CACCCTCCCC AGCCATCAAA 
CAGACCAGCC CTTGCAAGTC CAAGAGCCTT GGTGACTTGA CATCGGAAGA TATTTCCTGC 
AATTTTGAAA GTAAGTATCA GTACATAAGT AGGAGCTTTG TCACATCTGG CATGAGAGAC 
AAGAAGCTCG CTGCCATGAA GACGATGAGA CCGCACTCTA CGGATGCTTT GACGGAACAG 
CTGAGAAAGC TGCTGTCCCT GGACCAGGAC GACAGCCAGC AGATGCTGTA TTCCAGGCGG 
CTCGACGAGG ACTGCCCAAA GGCACTGGTC AGAAAGCTGT CGTCCAGGAG CCAGAGCAGA 
GTGCGGAACA TCGCCAGCCG TGCCAAGGAG AGGCAGGAGG CCAGCAAGCA AAAATCTTCC 
ACCGCAGGCA GCATAGCGGG GGTCGTCCTT CGGAACAAGC CTTCGGCGTC CCCTCACGTC 
GTCAACAGGC ACTCGACGGG CTCCTACATA GCCAGGTACC TGGGCAGCTT CCCTGAGGAG 
GCAGCAGAGG GCCGGGGCCG ACCTGAGGGC ACGTGTGCTG CCTGGCACCG CGGCTGTGCT 
GAGCGGCTGT GTGCGCACAG CCCCCTGCTG CAGCTGGAGC CCGACGGCGA CGAGAAGCCT 
GAAGTCTATT TCCTGCTGAG GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_025153771.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025009539.1 Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X6, mRNA.

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CloneID	OGa40159
Clone ID Related Accession (Same CDS sequence)	XM_025153771.1 , XM_015291819.1
Accession Version	XM_025153771.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGTAGA 
CTGACTCGTC GAAGGAAAAC TGTGAAGCTG TGCCGAGCTC TGTCTGACCT AGTGGTGTAC 
ACGAACTCTG TGGCAGCCCA GGATATAGTA GATGATGGAT CAACAGGGAA TGTGCTGTCA 
TTCAGTGAAA CAAGAGCCCA CCAAGCAGTG CAGCAGAAGG CTGAACAGTT CATGCTTTAC 
AACCAGAAAC AGCTTACAAG GGTTTATCCA TCAGCGTATC GGATTGACTC CAGCAATTTC 
AATCCTTTAC CTTACTGGAA TGTTGGTTGC CAGCTAGTGG CACTAAACTA CCAGTCTGAG 
GGACGTATGA TGCAATTAAA CGAAGCAAAA TTTAGGGTAA ATGGCAACTG TGGTTACGTC 
CTCAAACCTC AGCAGATGTG CAAAGATCCA CTTCCTGCTA GTCCTAAGAA GCAGCTCATC 
CTGAAAATCA TCAGTGGACA GCAGCTGCCT AAGCCTCCTG ACTCAATGCT AGGAGACAGA 
GGAGAGATAA TAGATCCCTT TGTCGAGGTG GAAATTATTG GGTTACCTGT TGACTGCTGT 
AAAGATCAGA CCAGGGTGGT TGATGACAAT GGGTTTAATC CTGTTTGGGA GGAAACGCTC 
ACTTTTACCA TCCATATGCC TGAAATAGCT TTGGTTCGAT TTCTTGTTTG GGATCATGAC 
CCCATTGGGC GAGACTTCGT TGGACAAAGA ACGCTGGCCT TTAGCAGTCT TGTACCTGGT 
TATCGACATG TGTATTTAGA AGGGCTGACA GAAGCTTCCA TATTTGTTCA CATAACCATT 
AATGAAATCT ATGGGAAGTG GAGCCCCTTA ATCCTCAATC CCAGTTACAC AATATTGCAC 
TTTCTAGGAG CCACAAAGAG TAAGCAACTG ATCGGACTCC GAGGGCTGTT TAACAAGAAC 
CCAAAGCACA GTTCTGCTGA AAGCAGCGGT CATTATGTAC GGAAGCGTTC TATTGGTGAC 
CGAATTCTCC GGCGCACGGC CAGTGCTCCA GCAAAAGGTA GAAAGAAAAG CAAGATGGGT 
TTTCTGGAAG CTTCTGAAAT TAAAGACAGT GCATCCGAAC CAGCAGACCT GAAGGACAAA 
GAAGGAGTTG TAAGGCGTAC AAGCCGGAGT TTGCAAGCAC GTCCTGCTTC TATGCCAGTG 
GACAAATTCC TTCTTGTAGG ACTGCCGTGC ACAGAAGATG AAACTGCTCA AGATGCCAAA 
GGAAAAGACA GTGCAACTGC CAACAGTGAT GGCGATAAAA CTGAGAAGGA AACAAACTTG 
AAGGAATCTC TTTTCACGTG TTCTGAGAAA ACTGCAGATC ATTCAAACTT GGCATCTCCC 
TTGAAAAAGG AGAGCGCCCA GAAGGAAACT ACAGCTGATT TTTCTCTTGT ACCCCAGGAG 
CAGCCCGAGC ATTCGGTTGC TGTAAGTGAT GTAACCGAAA CAAACCGCCC CATAAGCAAT 
CAGGAAAGTC TCCTTCCTGC TGAAACGGTG CCTCTGATTC CGGACTCTGC CTTTGAGCGC 
ATCACAGAAA AAATACAGGG CAAGAGCTGT GCAGGCAGTA GAAGGGCAGA TGACACAAAC 
AGGTGTGAGG AGAAAATAAC TCCTGTCAGG ACTTGCCTTC CTGAAAAAGT AGAGGAGGTT 
GAAAATCTCA AATACGACAT CATCCACAGA AGCACTGTGG CGTGTCTTTC TGTGACAGAT 
AGTTCTCCTG CCGTCTACTC TGATGCTCCT GGTTTATGCT CCTCTTCAAC CATGCAAGAC 
AGCGACATCT CCCGCCTCAT AGACGAAGTT TCTTTGGTGA CTGAGAGCGA GATGGACAGC 
GCTGTCTCAG CTCTGATCGG CCAGCTGGAT GTCACCGATG ACCAAACCAG CCTCACCGCA 
CCGATGCTCC CTGGCGCTGG CAGCCGGGCC TTCAGTGCTG TGGCCACACG CACACTGACT 
GCAGAGCTCA GCACTCCCTG TAAGAAGGAC TTCATTGCCA CACAGTCCAG CAAGTCCCCG 
TTATTTTCAA CTCCAGACTC TGCCGTGTTC CCCAGCCCCG AGACTGCGGA GTATTCTGTG 
TACACAATTA TCCACGAGGC AACTCTTACA CCCGGTTCTG AATTTAAGAC CCACAGTGGG 
TTAGTTTGCA AGAAGTCAAA GAGTGCAGAA AATAACTTGC CTAGCTCTTT TTGTTCTTCA 
CCTTTCTCTT TGCTGAATGC AAATCCCCAA AGAAAATGTG AAACAAGCTG GGAAGCCCCC 
GGCTCTGCGG GTTCTTTTGC TTCCAGTAGC CTCATCTTTG AGGAGATGCT TGTGGACCCT 
CCCATGGGCG AGAATTCGGA AAGCAGCAGT CTTGTAGAGT TAGGTGGGGA TTCCCAAGAC 
CTCTTGGTAA CTGCGTGCGC GTACAAAAGG GAAGATGTGA GCCAATTGGC TTCCCCTTTA 
AAACTGAGGC AGCAGCAGGA CAGCAGGTAC TGTGGTGAGA GGACCTTTGG AAACTGTGCA 
AGTGCTGGGC TCTGTGCTGC TGCCCTGGAC TGCTCGGATA ACTTTAGGAC TGCGGGAAGC 
AAGCTCCCTT TTCCAGCCTG TGAAAATGTT GGTTTTCTAC ATCATCGTCA CACAGATGAG 
CAGAAAGATT TGATGTGTAC CTCCAGGAGG TCCCAACTCG ATGTGCACTC ACCAGTGCTC 
AGAAGCGCGT TGGCTTTCCC ACCCTCCCCA GCCATCAAAC AGACCAGCCC TTGCAAGTCC 
AAGAGCCTTG GTGACTTGAC ATCGGAAGAT ATTTCCTGCA ATTTTGAAAG TAAGTATCAG 
TACATAAGTA GGAGCTTTGT CACATCTGGC ATGAGAGACA AGAAGCTCGC TGCCATGAAG 
ACGATGAGAC CGCACTCTAC GGATGCTTTG ACGGAACAGC TGAGAAAGCT GCTGTCCCTG 
GACCAGGACG ACAGCCAGCA GATGCTGTAT TCCAGGCGGC TCGACGAGGA CTGCCCAAAG 
GCACTGGTCA GAAAGCTGTC GTCCAGGAGC CAGAGCAGAG TGCGGAACAT CGCCAGCCGT 
GCCAAGGAGA GGCAGGAGGC CAGCAAGCAA AAATCTTCCA CCGCAGGCAG CATAGCGGGG 
GTCGTCCTTC GGAACAAGCC TTCGGCGTCC CCTCACGTCG TCAACAGGCA CTCGACGGGC 
TCCTACATAG CCAGGTACCT GGGCAGCTTC CCTGAGGAGG CAGCAGAGGG CCGGGGCCGA 
CCTGAGGGCA CGTGTGCTGC CTGGCACCGC GGCTGTGCTG AGCGGCTGTG TGCGCACAGC 
CCCCTGCTGC AGCTGGAGCC CGACGGCGAC GAGAAGCCTG AAGTCTATTT CCTGCTGAGG 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025009539.1
CDS	338..5443
Translation	MSYWSLDKKSCLQYCRHFLADNGVFHVERCMSVMQSGTQMVKLKSGTKGLVRLFYLDEHRTCIRWRPSRKSEKAKIVIDSIYKVTEGRQSEIFHRHAEGSFDPSCCFTIYHGNHLESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDHWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLNFEEFSVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTPEYCLDIIQKFEVSEENKEQNVLGIEGFTNFMRSPACDVFNPLHCEVHQDMDQPLCNYFIASSHNTYLTGDQLLSQSRVEMYARVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKIFFRDVAETINKYAFIKNEFPVILSIENHCSIQQQKKIAQYLKEILGDKLDLSSVVTGDSRQLPSPQNLKGKILVKGKKLPYSLGADAEEGEVSDEDSADEIEDDCKLKSCYSNGATEHQVESFIRTKLESLLKESQIRDKEDPDSFTVRALLRATHEGLSVNLKQNLDTKEGGKKSHSRSLMGNFGKHKKTGKATSKCSTSDDDENQQNSSGKEAGQLHSRLTRRRKTVKLCRALSDLVVYTNSVAAQDIVDDGSTGNVLSFSETRAHQAVQQKAEQFMLYNQKQLTRVYPSAYRIDSSNFNPLPYWNVGCQLVALNYQSEGRMMQLNEAKFRVNGNCGYVLKPQQMCKDPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTIHMPEIALVRFLVWDHDPIGRDFVGQRTLAFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSKQLIGLRGLFNKNPKHSSAESSGHYVRKRSIGDRILRRTASAPAKGRKKSKMGFLEASEIKDSASEPADLKDKEGVVRRTSRSLQARPASMPVDKFLLVGLPCTEDETAQDAKGKDSATANSDGDKTEKETNLKESLFTCSEKTADHSNLASPLKKESAQKETTADFSLVPQEQPEHSVAVSDVTETNRPISNQESLLPAETVPLIPDSAFERITEKIQGKSCAGSRRADDTNRCEEKITPVRTCLPEKVEEVENLKYDIIHRSTVACLSVTDSSPAVYSDAPGLCSSSTMQDSDISRLIDEVSLVTESEMDSAVSALIGQLDVTDDQTSLTAPMLPGAGSRAFSAVATRTLTAELSTPCKKDFIATQSSKSPLFSTPDSAVFPSPETAEYSVYTIIHEATLTPGSEFKTHSGLVCKKSKSAENNLPSSFCSSPFSLLNANPQRKCETSWEAPGSAGSFASSSLIFEEMLVDPPMGENSESSSLVELGGDSQDLLVTACAYKREDVSQLASPLKLRQQQDSRYCGERTFGNCASAGLCAAALDCSDNFRTAGSKLPFPACENVGFLHHRHTDEQKDLMCTSRRSQLDVHSPVLRSALAFPPSPAIKQTSPCKSKSLGDLTSEDISCNFESKYQYISRSFVTSGMRDKKLAAMKTMRPHSTDALTEQLRKLLSLDQDDSQQMLYSRRLDEDCPKALVRKLSSRSQSRVRNIASRAKERQEASKQKSSTAGSIAGVVLRNKPSASPHVVNRHSTGSYIARYLGSFPEEAAEGRGRPEGTCAAWHRGCAERLCAHSPLLQLEPDGDEKPEVYFLLRL

Target ORF information:

RefSeq Version	XM_025153771.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025153771.1

ORF Insert Sequence:

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGTAGA 
CTGACTCGTC GAAGGAAAAC TGTGAAGCTG TGCCGAGCTC TGTCTGACCT AGTGGTGTAC 
ACGAACTCTG TGGCAGCCCA GGATATAGTA GATGATGGAT CAACAGGGAA TGTGCTGTCA 
TTCAGTGAAA CAAGAGCCCA CCAAGCAGTG CAGCAGAAGG CTGAACAGTT CATGCTTTAC 
AACCAGAAAC AGCTTACAAG GGTTTATCCA TCAGCGTATC GGATTGACTC CAGCAATTTC 
AATCCTTTAC CTTACTGGAA TGTTGGTTGC CAGCTAGTGG CACTAAACTA CCAGTCTGAG 
GGACGTATGA TGCAATTAAA CGAAGCAAAA TTTAGGGTAA ATGGCAACTG TGGTTACGTC 
CTCAAACCTC AGCAGATGTG CAAAGATCCA CTTCCTGCTA GTCCTAAGAA GCAGCTCATC 
CTGAAAATCA TCAGTGGACA GCAGCTGCCT AAGCCTCCTG ACTCAATGCT AGGAGACAGA 
GGAGAGATAA TAGATCCCTT TGTCGAGGTG GAAATTATTG GGTTACCTGT TGACTGCTGT 
AAAGATCAGA CCAGGGTGGT TGATGACAAT GGGTTTAATC CTGTTTGGGA GGAAACGCTC 
ACTTTTACCA TCCATATGCC TGAAATAGCT TTGGTTCGAT TTCTTGTTTG GGATCATGAC 
CCCATTGGGC GAGACTTCGT TGGACAAAGA ACGCTGGCCT TTAGCAGTCT TGTACCTGGT 
TATCGACATG TGTATTTAGA AGGGCTGACA GAAGCTTCCA TATTTGTTCA CATAACCATT 
AATGAAATCT ATGGGAAGTG GAGCCCCTTA ATCCTCAATC CCAGTTACAC AATATTGCAC 
TTTCTAGGAG CCACAAAGAG TAAGCAACTG ATCGGACTCC GAGGGCTGTT TAACAAGAAC 
CCAAAGCACA GTTCTGCTGA AAGCAGCGGT CATTATGTAC GGAAGCGTTC TATTGGTGAC 
CGAATTCTCC GGCGCACGGC CAGTGCTCCA GCAAAAGGTA GAAAGAAAAG CAAGATGGGT 
TTTCTGGAAG CTTCTGAAAT TAAAGACAGT GCATCCGAAC CAGCAGACCT GAAGGACAAA 
GAAGGAGTTG TAAGGCGTAC AAGCCGGAGT TTGCAAGCAC GTCCTGCTTC TATGCCAGTG 
GACAAATTCC TTCTTGTAGG ACTGCCGTGC ACAGAAGATG AAACTGCTCA AGATGCCAAA 
GGAAAAGACA GTGCAACTGC CAACAGTGAT GGCGATAAAA CTGAGAAGGA AACAAACTTG 
AAGGAATCTC TTTTCACGTG TTCTGAGAAA ACTGCAGATC ATTCAAACTT GGCATCTCCC 
TTGAAAAAGG AGAGCGCCCA GAAGGAAACT ACAGCTGATT TTTCTCTTGT ACCCCAGGAG 
CAGCCCGAGC ATTCGGTTGC TGTAAGTGAT GTAACCGAAA CAAACCGCCC CATAAGCAAT 
CAGGAAAGTC TCCTTCCTGC TGAAACGGTG CCTCTGATTC CGGACTCTGC CTTTGAGCGC 
ATCACAGAAA AAATACAGGG CAAGAGCTGT GCAGGCAGTA GAAGGGCAGA TGACACAAAC 
AGGTGTGAGG AGAAAATAAC TCCTGTCAGG ACTTGCCTTC CTGAAAAAGT AGAGGAGGTT 
GAAAATCTCA AATACGACAT CATCCACAGA AGCACTGTGG CGTGTCTTTC TGTGACAGAT 
AGTTCTCCTG CCGTCTACTC TGATGCTCCT GGTTTATGCT CCTCTTCAAC CATGCAAGAC 
AGCGACATCT CCCGCCTCAT AGACGAAGTT TCTTTGGTGA CTGAGAGCGA GATGGACAGC 
GCTGTCTCAG CTCTGATCGG CCAGCTGGAT GTCACCGATG ACCAAACCAG CCTCACCGCA 
CCGATGCTCC CTGGCGCTGG CAGCCGGGCC TTCAGTGCTG TGGCCACACG CACACTGACT 
GCAGAGCTCA GCACTCCCTG TAAGAAGGAC TTCATTGCCA CACAGTCCAG CAAGTCCCCG 
TTATTTTCAA CTCCAGACTC TGCCGTGTTC CCCAGCCCCG AGACTGCGGA GTATTCTGTG 
TACACAATTA TCCACGAGGC AACTCTTACA CCCGGTTCTG AATTTAAGAC CCACAGTGGG 
TTAGTTTGCA AGAAGTCAAA GAGTGCAGAA AATAACTTGC CTAGCTCTTT TTGTTCTTCA 
CCTTTCTCTT TGCTGAATGC AAATCCCCAA AGAAAATGTG AAACAAGCTG GGAAGCCCCC 
GGCTCTGCGG GTTCTTTTGC TTCCAGTAGC CTCATCTTTG AGGAGATGCT TGTGGACCCT 
CCCATGGGCG AGAATTCGGA AAGCAGCAGT CTTGTAGAGT TAGGTGGGGA TTCCCAAGAC 
CTCTTGGTAA CTGCGTGCGC GTACAAAAGG GAAGATGTGA GCCAATTGGC TTCCCCTTTA 
AAACTGAGGC AGCAGCAGGA CAGCAGGTAC TGTGGTGAGA GGACCTTTGG AAACTGTGCA 
AGTGCTGGGC TCTGTGCTGC TGCCCTGGAC TGCTCGGATA ACTTTAGGAC TGCGGGAAGC 
AAGCTCCCTT TTCCAGCCTG TGAAAATGTT GGTTTTCTAC ATCATCGTCA CACAGATGAG 
CAGAAAGATT TGATGTGTAC CTCCAGGAGG TCCCAACTCG ATGTGCACTC ACCAGTGCTC 
AGAAGCGCGT TGGCTTTCCC ACCCTCCCCA GCCATCAAAC AGACCAGCCC TTGCAAGTCC 
AAGAGCCTTG GTGACTTGAC ATCGGAAGAT ATTTCCTGCA ATTTTGAAAG TAAGTATCAG 
TACATAAGTA GGAGCTTTGT CACATCTGGC ATGAGAGACA AGAAGCTCGC TGCCATGAAG 
ACGATGAGAC CGCACTCTAC GGATGCTTTG ACGGAACAGC TGAGAAAGCT GCTGTCCCTG 
GACCAGGACG ACAGCCAGCA GATGCTGTAT TCCAGGCGGC TCGACGAGGA CTGCCCAAAG 
GCACTGGTCA GAAAGCTGTC GTCCAGGAGC CAGAGCAGAG TGCGGAACAT CGCCAGCCGT 
GCCAAGGAGA GGCAGGAGGC CAGCAAGCAA AAATCTTCCA CCGCAGGCAG CATAGCGGGG 
GTCGTCCTTC GGAACAAGCC TTCGGCGTCC CCTCACGTCG TCAACAGGCA CTCGACGGGC 
TCCTACATAG CCAGGTACCT GGGCAGCTTC CCTGAGGAGG CAGCAGAGGG CCGGGGCCGA 
CCTGAGGGCA CGTGTGCTGC CTGGCACCGC GGCTGTGCTG AGCGGCTGTG TGCGCACAGC 
CCCCTGCTGC AGCTGGAGCC CGACGGCGAC GAGAAGCCTG AAGTCTATTT CCTGCTGAGG 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_015291819.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147305.1 Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OGa40159
Clone ID Related Accession (Same CDS sequence)	XM_025153771.1 , XM_015291819.1
Accession Version	XM_015291819.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGTAGA 
CTGACTCGTC GAAGGAAAAC TGTGAAGCTG TGCCGAGCTC TGTCTGACCT AGTGGTGTAC 
ACGAACTCTG TGGCAGCCCA GGATATAGTA GATGATGGAT CAACAGGGAA TGTGCTGTCA 
TTCAGTGAAA CAAGAGCCCA CCAAGCAGTG CAGCAGAAGG CTGAACAGTT CATGCTTTAC 
AACCAGAAAC AGCTTACAAG GGTTTATCCA TCAGCGTATC GGATTGACTC CAGCAATTTC 
AATCCTTTAC CTTACTGGAA TGTTGGTTGC CAGCTAGTGG CACTAAACTA CCAGTCTGAG 
GGACGTATGA TGCAATTAAA CGAAGCAAAA TTTAGGGTAA ATGGCAACTG TGGTTACGTC 
CTCAAACCTC AGCAGATGTG CAAAGATCCA CTTCCTGCTA GTCCTAAGAA GCAGCTCATC 
CTGAAAATCA TCAGTGGACA GCAGCTGCCT AAGCCTCCTG ACTCAATGCT AGGAGACAGA 
GGAGAGATAA TAGATCCCTT TGTCGAGGTG GAAATTATTG GGTTACCTGT TGACTGCTGT 
AAAGATCAGA CCAGGGTGGT TGATGACAAT GGGTTTAATC CTGTTTGGGA GGAAACGCTC 
ACTTTTACCA TCCATATGCC TGAAATAGCT TTGGTTCGAT TTCTTGTTTG GGATCATGAC 
CCCATTGGGC GAGACTTCGT TGGACAAAGA ACGCTGGCCT TTAGCAGTCT TGTACCTGGT 
TATCGACATG TGTATTTAGA AGGGCTGACA GAAGCTTCCA TATTTGTTCA CATAACCATT 
AATGAAATCT ATGGGAAGTG GAGCCCCTTA ATCCTCAATC CCAGTTACAC AATATTGCAC 
TTTCTAGGAG CCACAAAGAG TAAGCAACTG ATCGGACTCC GAGGGCTGTT TAACAAGAAC 
CCAAAGCACA GTTCTGCTGA AAGCAGCGGT CATTATGTAC GGAAGCGTTC TATTGGTGAC 
CGAATTCTCC GGCGCACGGC CAGTGCTCCA GCAAAAGGTA GAAAGAAAAG CAAGATGGGT 
TTTCTGGAAG CTTCTGAAAT TAAAGACAGT GCATCCGAAC CAGCAGACCT GAAGGACAAA 
GAAGGAGTTG TAAGGCGTAC AAGCCGGAGT TTGCAAGCAC GTCCTGCTTC TATGCCAGTG 
GACAAATTCC TTCTTGTAGG ACTGCCGTGC ACAGAAGATG AAACTGCTCA AGATGCCAAA 
GGAAAAGACA GTGCAACTGC CAACAGTGAT GGCGATAAAA CTGAGAAGGA AACAAACTTG 
AAGGAATCTC TTTTCACGTG TTCTGAGAAA ACTGCAGATC ATTCAAACTT GGCATCTCCC 
TTGAAAAAGG AGAGCGCCCA GAAGGAAACT ACAGCTGATT TTTCTCTTGT ACCCCAGGAG 
CAGCCCGAGC ATTCGGTTGC TGTAAGTGAT GTAACCGAAA CAAACCGCCC CATAAGCAAT 
CAGGAAAGTC TCCTTCCTGC TGAAACGGTG CCTCTGATTC CGGACTCTGC CTTTGAGCGC 
ATCACAGAAA AAATACAGGG CAAGAGCTGT GCAGGCAGTA GAAGGGCAGA TGACACAAAC 
AGGTGTGAGG AGAAAATAAC TCCTGTCAGG ACTTGCCTTC CTGAAAAAGT AGAGGAGGTT 
GAAAATCTCA AATACGACAT CATCCACAGA AGCACTGTGG CGTGTCTTTC TGTGACAGAT 
AGTTCTCCTG CCGTCTACTC TGATGCTCCT GGTTTATGCT CCTCTTCAAC CATGCAAGAC 
AGCGACATCT CCCGCCTCAT AGACGAAGTT TCTTTGGTGA CTGAGAGCGA GATGGACAGC 
GCTGTCTCAG CTCTGATCGG CCAGCTGGAT GTCACCGATG ACCAAACCAG CCTCACCGCA 
CCGATGCTCC CTGGCGCTGG CAGCCGGGCC TTCAGTGCTG TGGCCACACG CACACTGACT 
GCAGAGCTCA GCACTCCCTG TAAGAAGGAC TTCATTGCCA CACAGTCCAG CAAGTCCCCG 
TTATTTTCAA CTCCAGACTC TGCCGTGTTC CCCAGCCCCG AGACTGCGGA GTATTCTGTG 
TACACAATTA TCCACGAGGC AACTCTTACA CCCGGTTCTG AATTTAAGAC CCACAGTGGG 
TTAGTTTGCA AGAAGTCAAA GAGTGCAGAA AATAACTTGC CTAGCTCTTT TTGTTCTTCA 
CCTTTCTCTT TGCTGAATGC AAATCCCCAA AGAAAATGTG AAACAAGCTG GGAAGCCCCC 
GGCTCTGCGG GTTCTTTTGC TTCCAGTAGC CTCATCTTTG AGGAGATGCT TGTGGACCCT 
CCCATGGGCG AGAATTCGGA AAGCAGCAGT CTTGTAGAGT TAGGTGGGGA TTCCCAAGAC 
CTCTTGGTAA CTGCGTGCGC GTACAAAAGG GAAGATGTGA GCCAATTGGC TTCCCCTTTA 
AAACTGAGGC AGCAGCAGGA CAGCAGGTAC TGTGGTGAGA GGACCTTTGG AAACTGTGCA 
AGTGCTGGGC TCTGTGCTGC TGCCCTGGAC TGCTCGGATA ACTTTAGGAC TGCGGGAAGC 
AAGCTCCCTT TTCCAGCCTG TGAAAATGTT GGTTTTCTAC ATCATCGTCA CACAGATGAG 
CAGAAAGATT TGATGTGTAC CTCCAGGAGG TCCCAACTCG ATGTGCACTC ACCAGTGCTC 
AGAAGCGCGT TGGCTTTCCC ACCCTCCCCA GCCATCAAAC AGACCAGCCC TTGCAAGTCC 
AAGAGCCTTG GTGACTTGAC ATCGGAAGAT ATTTCCTGCA ATTTTGAAAG TAAGTATCAG 
TACATAAGTA GGAGCTTTGT CACATCTGGC ATGAGAGACA AGAAGCTCGC TGCCATGAAG 
ACGATGAGAC CGCACTCTAC GGATGCTTTG ACGGAACAGC TGAGAAAGCT GCTGTCCCTG 
GACCAGGACG ACAGCCAGCA GATGCTGTAT TCCAGGCGGC TCGACGAGGA CTGCCCAAAG 
GCACTGGTCA GAAAGCTGTC GTCCAGGAGC CAGAGCAGAG TGCGGAACAT CGCCAGCCGT 
GCCAAGGAGA GGCAGGAGGC CAGCAAGCAA AAATCTTCCA CCGCAGGCAG CATAGCGGGG 
GTCGTCCTTC GGAACAAGCC TTCGGCGTCC CCTCACGTCG TCAACAGGCA CTCGACGGGC 
TCCTACATAG CCAGGTACCT GGGCAGCTTC CCTGAGGAGG CAGCAGAGGG CCGGGGCCGA 
CCTGAGGGCA CGTGTGCTGC CTGGCACCGC GGCTGTGCTG AGCGGCTGTG TGCGCACAGC 
CCCCTGCTGC AGCTGGAGCC CGACGGCGAC GAGAAGCCTG AAGTCTATTT CCTGCTGAGG 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147305.1
CDS	1382..6487
Translation	MSYWSLDKKSCLQYCRHFLADNGVFHVERCMSVMQSGTQMVKLKSGTKGLVRLFYLDEHRTCIRWRPSRKSEKAKIVIDSIYKVTEGRQSEIFHRHAEGSFDPSCCFTIYHGNHLESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDHWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLNFEEFSVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTPEYCLDIIQKFEVSEENKEQNVLGIEGFTNFMRSPACDVFNPLHCEVHQDMDQPLCNYFIASSHNTYLTGDQLLSQSRVEMYARVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKIFFRDVAETINKYAFIKNEFPVILSIENHCSIQQQKKIAQYLKEILGDKLDLSSVVTGDSRQLPSPQNLKGKILVKGKKLPYSLGADAEEGEVSDEDSADEIEDDCKLKSCYSNGATEHQVESFIRTKLESLLKESQIRDKEDPDSFTVRALLRATHEGLSVNLKQNLDTKEGGKKSHSRSLMGNFGKHKKTGKATSKCSTSDDDENQQNSSGKEAGQLHSRLTRRRKTVKLCRALSDLVVYTNSVAAQDIVDDGSTGNVLSFSETRAHQAVQQKAEQFMLYNQKQLTRVYPSAYRIDSSNFNPLPYWNVGCQLVALNYQSEGRMMQLNEAKFRVNGNCGYVLKPQQMCKDPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTIHMPEIALVRFLVWDHDPIGRDFVGQRTLAFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSKQLIGLRGLFNKNPKHSSAESSGHYVRKRSIGDRILRRTASAPAKGRKKSKMGFLEASEIKDSASEPADLKDKEGVVRRTSRSLQARPASMPVDKFLLVGLPCTEDETAQDAKGKDSATANSDGDKTEKETNLKESLFTCSEKTADHSNLASPLKKESAQKETTADFSLVPQEQPEHSVAVSDVTETNRPISNQESLLPAETVPLIPDSAFERITEKIQGKSCAGSRRADDTNRCEEKITPVRTCLPEKVEEVENLKYDIIHRSTVACLSVTDSSPAVYSDAPGLCSSSTMQDSDISRLIDEVSLVTESEMDSAVSALIGQLDVTDDQTSLTAPMLPGAGSRAFSAVATRTLTAELSTPCKKDFIATQSSKSPLFSTPDSAVFPSPETAEYSVYTIIHEATLTPGSEFKTHSGLVCKKSKSAENNLPSSFCSSPFSLLNANPQRKCETSWEAPGSAGSFASSSLIFEEMLVDPPMGENSESSSLVELGGDSQDLLVTACAYKREDVSQLASPLKLRQQQDSRYCGERTFGNCASAGLCAAALDCSDNFRTAGSKLPFPACENVGFLHHRHTDEQKDLMCTSRRSQLDVHSPVLRSALAFPPSPAIKQTSPCKSKSLGDLTSEDISCNFESKYQYISRSFVTSGMRDKKLAAMKTMRPHSTDALTEQLRKLLSLDQDDSQQMLYSRRLDEDCPKALVRKLSSRSQSRVRNIASRAKERQEASKQKSSTAGSIAGVVLRNKPSASPHVVNRHSTGSYIARYLGSFPEEAAEGRGRPEGTCAAWHRGCAERLCAHSPLLQLEPDGDEKPEVYFLLRL

Target ORF information:

RefSeq Version	XM_015291819.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291819.1

ORF Insert Sequence:

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGTAGA 
CTGACTCGTC GAAGGAAAAC TGTGAAGCTG TGCCGAGCTC TGTCTGACCT AGTGGTGTAC 
ACGAACTCTG TGGCAGCCCA GGATATAGTA GATGATGGAT CAACAGGGAA TGTGCTGTCA 
TTCAGTGAAA CAAGAGCCCA CCAAGCAGTG CAGCAGAAGG CTGAACAGTT CATGCTTTAC 
AACCAGAAAC AGCTTACAAG GGTTTATCCA TCAGCGTATC GGATTGACTC CAGCAATTTC 
AATCCTTTAC CTTACTGGAA TGTTGGTTGC CAGCTAGTGG CACTAAACTA CCAGTCTGAG 
GGACGTATGA TGCAATTAAA CGAAGCAAAA TTTAGGGTAA ATGGCAACTG TGGTTACGTC 
CTCAAACCTC AGCAGATGTG CAAAGATCCA CTTCCTGCTA GTCCTAAGAA GCAGCTCATC 
CTGAAAATCA TCAGTGGACA GCAGCTGCCT AAGCCTCCTG ACTCAATGCT AGGAGACAGA 
GGAGAGATAA TAGATCCCTT TGTCGAGGTG GAAATTATTG GGTTACCTGT TGACTGCTGT 
AAAGATCAGA CCAGGGTGGT TGATGACAAT GGGTTTAATC CTGTTTGGGA GGAAACGCTC 
ACTTTTACCA TCCATATGCC TGAAATAGCT TTGGTTCGAT TTCTTGTTTG GGATCATGAC 
CCCATTGGGC GAGACTTCGT TGGACAAAGA ACGCTGGCCT TTAGCAGTCT TGTACCTGGT 
TATCGACATG TGTATTTAGA AGGGCTGACA GAAGCTTCCA TATTTGTTCA CATAACCATT 
AATGAAATCT ATGGGAAGTG GAGCCCCTTA ATCCTCAATC CCAGTTACAC AATATTGCAC 
TTTCTAGGAG CCACAAAGAG TAAGCAACTG ATCGGACTCC GAGGGCTGTT TAACAAGAAC 
CCAAAGCACA GTTCTGCTGA AAGCAGCGGT CATTATGTAC GGAAGCGTTC TATTGGTGAC 
CGAATTCTCC GGCGCACGGC CAGTGCTCCA GCAAAAGGTA GAAAGAAAAG CAAGATGGGT 
TTTCTGGAAG CTTCTGAAAT TAAAGACAGT GCATCCGAAC CAGCAGACCT GAAGGACAAA 
GAAGGAGTTG TAAGGCGTAC AAGCCGGAGT TTGCAAGCAC GTCCTGCTTC TATGCCAGTG 
GACAAATTCC TTCTTGTAGG ACTGCCGTGC ACAGAAGATG AAACTGCTCA AGATGCCAAA 
GGAAAAGACA GTGCAACTGC CAACAGTGAT GGCGATAAAA CTGAGAAGGA AACAAACTTG 
AAGGAATCTC TTTTCACGTG TTCTGAGAAA ACTGCAGATC ATTCAAACTT GGCATCTCCC 
TTGAAAAAGG AGAGCGCCCA GAAGGAAACT ACAGCTGATT TTTCTCTTGT ACCCCAGGAG 
CAGCCCGAGC ATTCGGTTGC TGTAAGTGAT GTAACCGAAA CAAACCGCCC CATAAGCAAT 
CAGGAAAGTC TCCTTCCTGC TGAAACGGTG CCTCTGATTC CGGACTCTGC CTTTGAGCGC 
ATCACAGAAA AAATACAGGG CAAGAGCTGT GCAGGCAGTA GAAGGGCAGA TGACACAAAC 
AGGTGTGAGG AGAAAATAAC TCCTGTCAGG ACTTGCCTTC CTGAAAAAGT AGAGGAGGTT 
GAAAATCTCA AATACGACAT CATCCACAGA AGCACTGTGG CGTGTCTTTC TGTGACAGAT 
AGTTCTCCTG CCGTCTACTC TGATGCTCCT GGTTTATGCT CCTCTTCAAC CATGCAAGAC 
AGCGACATCT CCCGCCTCAT AGACGAAGTT TCTTTGGTGA CTGAGAGCGA GATGGACAGC 
GCTGTCTCAG CTCTGATCGG CCAGCTGGAT GTCACCGATG ACCAAACCAG CCTCACCGCA 
CCGATGCTCC CTGGCGCTGG CAGCCGGGCC TTCAGTGCTG TGGCCACACG CACACTGACT 
GCAGAGCTCA GCACTCCCTG TAAGAAGGAC TTCATTGCCA CACAGTCCAG CAAGTCCCCG 
TTATTTTCAA CTCCAGACTC TGCCGTGTTC CCCAGCCCCG AGACTGCGGA GTATTCTGTG 
TACACAATTA TCCACGAGGC AACTCTTACA CCCGGTTCTG AATTTAAGAC CCACAGTGGG 
TTAGTTTGCA AGAAGTCAAA GAGTGCAGAA AATAACTTGC CTAGCTCTTT TTGTTCTTCA 
CCTTTCTCTT TGCTGAATGC AAATCCCCAA AGAAAATGTG AAACAAGCTG GGAAGCCCCC 
GGCTCTGCGG GTTCTTTTGC TTCCAGTAGC CTCATCTTTG AGGAGATGCT TGTGGACCCT 
CCCATGGGCG AGAATTCGGA AAGCAGCAGT CTTGTAGAGT TAGGTGGGGA TTCCCAAGAC 
CTCTTGGTAA CTGCGTGCGC GTACAAAAGG GAAGATGTGA GCCAATTGGC TTCCCCTTTA 
AAACTGAGGC AGCAGCAGGA CAGCAGGTAC TGTGGTGAGA GGACCTTTGG AAACTGTGCA 
AGTGCTGGGC TCTGTGCTGC TGCCCTGGAC TGCTCGGATA ACTTTAGGAC TGCGGGAAGC 
AAGCTCCCTT TTCCAGCCTG TGAAAATGTT GGTTTTCTAC ATCATCGTCA CACAGATGAG 
CAGAAAGATT TGATGTGTAC CTCCAGGAGG TCCCAACTCG ATGTGCACTC ACCAGTGCTC 
AGAAGCGCGT TGGCTTTCCC ACCCTCCCCA GCCATCAAAC AGACCAGCCC TTGCAAGTCC 
AAGAGCCTTG GTGACTTGAC ATCGGAAGAT ATTTCCTGCA ATTTTGAAAG TAAGTATCAG 
TACATAAGTA GGAGCTTTGT CACATCTGGC ATGAGAGACA AGAAGCTCGC TGCCATGAAG 
ACGATGAGAC CGCACTCTAC GGATGCTTTG ACGGAACAGC TGAGAAAGCT GCTGTCCCTG 
GACCAGGACG ACAGCCAGCA GATGCTGTAT TCCAGGCGGC TCGACGAGGA CTGCCCAAAG 
GCACTGGTCA GAAAGCTGTC GTCCAGGAGC CAGAGCAGAG TGCGGAACAT CGCCAGCCGT 
GCCAAGGAGA GGCAGGAGGC CAGCAAGCAA AAATCTTCCA CCGCAGGCAG CATAGCGGGG 
GTCGTCCTTC GGAACAAGCC TTCGGCGTCC CCTCACGTCG TCAACAGGCA CTCGACGGGC 
TCCTACATAG CCAGGTACCT GGGCAGCTTC CCTGAGGAGG CAGCAGAGGG CCGGGGCCGA 
CCTGAGGGCA CGTGTGCTGC CTGGCACCGC GGCTGTGCTG AGCGGCTGTG TGCGCACAGC 
CCCCTGCTGC AGCTGGAGCC CGACGGCGAC GAGAAGCCTG AAGTCTATTT CCTGCTGAGG 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_015291820.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147306.1 Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X7, mRNA.

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CloneID	OGa40160
Clone ID Related Accession (Same CDS sequence)	XM_015291820.2 , XM_015291820.1
Accession Version	XM_015291820.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291820.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCT GCAGGAGGAC TCTGCTCCCC ACCTGCCCGC GGCTCGGCCT TGAAAGATGC 
ATGAGTGTTA TGCAGTCTGG GACTCAGATG GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC 
GTTCGTCTCT TCTACCTGGA CGAGCACAGG ACCTGCATCC GATGGAGACC GTCCAGAAAA 
AGTGAAAAGG CAAAAATTGT CATTGATTCT ATTTACAAAG TGACCGAAGG CCGTCAGTCT 
GAAATCTTCC ACCGCCATGC AGAGGGGAGC TTTGACCCGA GCTGTTGCTT TACCATTTAC 
CATGGCAACC ACCTGGAGTC ACTGGATCTG ATTACCTCTA ACCCAGAGGA AGCTCGCACC 
TGGATCACAG GGCTGAAATA TTTGATGGCT GGGATAAGCG ATGAGGACTC GCTTGCTAAG 
CGACAGAGAA CTCATGATCA CTGGGTGAAA CAAACTTTTG AGGAAGCTGA CAAGAATGGA 
GATGGCCTGC TGAACATTGA AGAGATCCAC CAGCTGATGC ATAAGCTAAA TGTTAACCTG 
CCCCGCAGGA AGGTCCGGCA GATGTTTCAG GAAGCTGACA CAGATGAGAA CCAAGGAACT 
CTAAATTTTG AAGAATTTTC AGTGTTTTAC AAGATGATGT CCTTGCGTCG AGACCTGTAC 
TTACTGCTCT TAAGCTACAG TGACAAGAAA GATCACCTCA CCGTTGAAGA ACTTGCTCAA 
TTTCTGAAGG TGGAACAAAA GATGAGTAAC GTGACGCCAG AATATTGTCT TGACATCATA 
CAGAAGTTTG AAGTATCAGA AGAAAACAAG GAGCAGAACG TGCTTGGGAT TGAAGGTTTC 
ACCAACTTCA TGCGCAGTCC TGCTTGTGAT GTTTTTAATC CATTGCATTG CGAAGTGCAT 
CAGGATATGG ATCAACCCCT TTGTAACTAC TTCATTGCTT CATCTCACAA CACGTACCTG 
ACAGGAGACC AGCTGCTGTC CCAGTCTAGG GTGGAGATGT ATGCACGAGT GCTGCAGGAC 
GGTTGTCGAT GTATTGAAGT GGATTGCTGG GATGGTCCAG ATGGAGAGCC AGTAGTGCAT 
CATGGGTATA CGCTTACCTC TAAAATATTC TTCCGTGACG TGGCAGAAAC TATCAATAAG 
TATGCCTTCA TCAAAAATGA GTTTCCGGTG ATACTGTCTA TTGAAAATCA TTGCAGTATT 
CAGCAGCAAA AGAAGATTGC TCAGTACTTG AAGGAGATAC TTGGTGACAA ATTGGACTTG 
TCATCTGTGG TCACCGGAGA TTCCAGGCAG CTTCCTAGCC CTCAAAACTT GAAAGGGAAA 
ATCCTAGTGA AGGGAAAAAA GTTGCCATAC AGTCTTGGAG CAGATGCTGA GGAAGGAGAA 
GTTTCTGATG AGGACAGTGC TGATGAAATT GAAGACGACT GCAAGCTGAA ATCCTGCTAT 
AGTAACGGTG CAACTGAGCA TCAGGTGGAA TCTTTTATAA GAACAAAGCT TGAATCCCTG 
CTAAAGGAGT CCCAGATCAG GGACAAAGAA GATCCTGACA GCTTCACGGT GAGAGCACTG 
CTAAGGGCAA CTCACGAGGG TTTAAGTGTT AACCTGAAGC AGAACCTGGA CACAAAAGAA 
GGTGGGAAGA AGTCTCATAG TCGATCATTA ATGGGCAACT TTGGTAAACA CAAAAAAACA 
GGGAAGGCAA CATCCAAATG TAGCACATCA GATGATGATG AAAATCAGCA AAACTCTTCT 
GGAAAGGAAG CAGGACAGCT GCACAGTAGA CTGACTCGTC GAAGGAAAAC TGTGAAGCTG 
TGCCGAGCTC TGTCTGACCT AGTGGTGTAC ACGAACTCTG TGGCAGCCCA GGATATAGTA 
GATGATGGAT CAACAGGGAA TGTGCTGTCA TTCAGTGAAA CAAGAGCCCA CCAAGCAGTG 
CAGCAGAAGG CTGAACAGTT CATGCTTTAC AACCAGAAAC AGCTTACAAG GGTTTATCCA 
TCAGCGTATC GGATTGACTC CAGCAATTTC AATCCTTTAC CTTACTGGAA TGTTGGTTGC 
CAGCTAGTGG CACTAAACTA CCAGTCTGAG GGACGTATGA TGCAATTAAA CGAAGCAAAA 
TTTAGGGTAA ATGGCAACTG TGGTTACGTC CTCAAACCTC AGCAGATGTG CAAAGGCTCA 
TTCAACCCCT ATTCTTCAGA TCCACTTCCT GCTAGTCCTA AGAAGCAGCT CATCCTGAAA 
ATCATCAGTG GACAGCAGCT GCCTAAGCCT CCTGACTCAA TGCTAGGAGA CAGAGGAGAG 
ATAATAGATC CCTTTGTCGA GGTGGAAATT ATTGGGTTAC CTGTTGACTG CTGTAAAGAT 
CAGACCAGGG TGGTTGATGA CAATGGGTTT AATCCTGTTT GGGAGGAAAC GCTCACTTTT 
ACCATCCATA TGCCTGAAAT AGCTTTGGTT CGATTTCTTG TTTGGGATCA TGACCCCATT 
GGGCGAGACT TCGTTGGACA AAGAACGCTG GCCTTTAGCA GTCTTGTACC TGGTTATCGA 
CATGTGTATT TAGAAGGGCT GACAGAAGCT TCCATATTTG TTCACATAAC CATTAATGAA 
ATCTATGGGA AGTGGAGCCC CTTAATCCTC AATCCCAGTT ACACAATATT GCACTTTCTA 
GGAGCCACAA AGAGTAAGCA ACTGATCGGA CTCCGAGGGC TGTTTAACAA GAACCCAAAG 
CACAGTTCTG CTGAAAGCAG CGGTCATTAT GTACGGAAGC GTTCTATTGG TGACCGAATT 
CTCCGGCGCA CGGCCAGTGC TCCAGCAAAA GGTAGAAAGA AAAGCAAGAT GGGTTTTCTG 
GAAGCTTCTG AAATTAAAGA CAGTGCATCC GAACCAGCAG ACCTGAAGGA CAAAGAAGGA 
GTTGTAAGGC GTACAAGCCG GAGTTTGCAA GCACGTCCTG CTTCTATGCC AGTGGACAAA 
TTCCTTCTTG TAGGACTGCC GTGCACAGAA GATGAAACTG CTCAAGATGC CAAAGGAAAA 
GACAGTGCAA CTGCCAACAG TGATGGCGAT AAAACTGAGA AGGAAACAAA CTTGAAGGAA 
TCTCTTTTCA CGTGTTCTGA GAAAACTGCA GATCATTCAA ACTTGGCATC TCCCTTGAAA 
AAGGAGAGCG CCCAGAAGGA AACTACAGCT GATTTTTCTC TTGTACCCCA GGAGCAGCCC 
GAGCATTCGG TTGCTGTAAG TGATGTAACC GAAACAAACC GCCCCATAAG CAATCAGGAA 
AGTCTCCTTC CTGCTGAAAC GGTGCCTCTG ATTCCGGACT CTGCCTTTGA GCGCATCACA 
GAAAAAATAC AGGGCAAGAG CTGTGCAGGC AGTAGAAGGG CAGATGACAC AAACAGGTGT 
GAGGAGAAAA TAACTCCTGT CAGGACTTGC CTTCCTGAAA AAGTAGAGGA GGTTGAAAAT 
CTCAAATACG ACATCATCCA CAGAAGCACT GTGGCGTGTC TTTCTGTGAC AGATAGTTCT 
CCTGCCGTCT ACTCTGATGC TCCTGGTTTA TGCTCCTCTT CAACCATGCA AGACAGCGAC 
ATCTCCCGCC TCATAGACGA AGTTTCTTTG GTGACTGAGA GCGAGATGGA CAGCGCTGTC 
TCAGCTCTGA TCGGCCAGCT GGATGTCACC GATGACCAAA CCAGCCTCAC CGCACCGATG 
CTCCCTGGCG CTGGCAGCCG GGCCTTCAGT GCTGTGGCCA CACGCACACT GACTGCAGAG 
CTCAGCACTC CCTGTAAGAA GGACTTCATT GCCACACAGT CCAGCAAGTC CCCGTTATTT 
TCAACTCCAG ACTCTGCCGT GTTCCCCAGC CCCGAGACTG CGGAGTATTC TGTGTACACA 
ATTATCCACG AGGCAACTCT TACACCCGGT TCTGAATTTA AGACCCACAG TGGGTTAGTT 
TGCAAGAAGT CAAAGAGTGC AGAAAATAAC TTGCCTAGCT CTTTTTGTTC TTCACCTTTC 
TCTTTGCTGA ATGCAAATCC CCAAAGAAAA TGTGAAACAA GCTGGGAAGC CCCCGGCTCT 
GCGGGTTCTT TTGCTTCCAG TAGCCTCATC TTTGAGGAGA TGCTTGTGGA CCCTCCCATG 
GGCGAGAATT CGGAAAGCAG CAGTCTTGTA GAGTTAGGTG GGGATTCCCA AGACCTCTTG 
GTAACTGCGT GCGCGTACAA AAGGGAAGAT GTGAGCCAAT TGGCTTCCCC TTTAAAACTG 
AGGCAGCAGC AGGACAGCAG GTACTGTGGT GAGAGGACCT TTGGAAACTG TGCAAGTGCT 
GGGCTCTGTG CTGCTGCCCT GGACTGCTCG GATAACTTTA GGACTGCGGG AAGCAAGCTC 
CCTTTTCCAG CCTGTGAAAA TGTTGGTTTT CTACATCATC GTCACACAGA TGAGCAGAAA 
GATTTGATGT GTACCTCCAG GAGGTCCCAA CTCGATGTGC ACTCACCAGT GCTCAGAAGC 
GCGTTGGCTT TCCCACCCTC CCCAGCCATC AAACAGACCA GCCCTTGCAA GTCCAAGAGC 
CTTGGTGACT TGACATCGGA AGATATTTCC TGCAATTTTG AAAGTAAGTA TCAGTACATA 
AGTAGGAGCT TTGTCACATC TGGCATGAGA GACAAGAAGC TCGCTGCCAT GAAGACGATG 
AGACCGCACT CTACGGATGC TTTGACGGAA CAGCTGAGAA AGCTGCTGTC CCTGGACCAG 
GACGACAGCC AGCAGATGCT GTATTCCAGG CGGCTCGACG AGGACTGCCC AAAGGCACTG 
GTCAGAAAGC TGTCGTCCAG GAGCCAGAGC AGAGTGCGGA ACATCGCCAG CCGTGCCAAG 
GAGAGGCAGG AGGCCAGCAA GCAAAAATCT TCCACCGCAG GCAGCATAGC GGGGGTCGTC 
CTTCGGAACA AGCCTTCGGC GTCCCCTCAC GTCGTCAACA GGCACTCGAC GGGCTCCTAC 
ATAGCCAGGT ACCTGGGCAG CTTCCCTGAG GAGGCAGCAG AGGGCCGGGG CCGACCTGAG 
GGCACGTGTG CTGCCTGGCA CCGCGGCTGT GCTGAGCGGC TGTGTGCGCA CAGCCCCCTG 
CTGCAGCTGG AGCCCGACGG CGACGAGAAG CCTGAAGTCT ATTTCCTGCT GAGGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147306.1
CDS	59..5158
Translation	MSGCRRTLLPTCPRLGLERCMSVMQSGTQMVKLKSGTKGLVRLFYLDEHRTCIRWRPSRKSEKAKIVIDSIYKVTEGRQSEIFHRHAEGSFDPSCCFTIYHGNHLESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDHWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLNFEEFSVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTPEYCLDIIQKFEVSEENKEQNVLGIEGFTNFMRSPACDVFNPLHCEVHQDMDQPLCNYFIASSHNTYLTGDQLLSQSRVEMYARVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKIFFRDVAETINKYAFIKNEFPVILSIENHCSIQQQKKIAQYLKEILGDKLDLSSVVTGDSRQLPSPQNLKGKILVKGKKLPYSLGADAEEGEVSDEDSADEIEDDCKLKSCYSNGATEHQVESFIRTKLESLLKESQIRDKEDPDSFTVRALLRATHEGLSVNLKQNLDTKEGGKKSHSRSLMGNFGKHKKTGKATSKCSTSDDDENQQNSSGKEAGQLHSRLTRRRKTVKLCRALSDLVVYTNSVAAQDIVDDGSTGNVLSFSETRAHQAVQQKAEQFMLYNQKQLTRVYPSAYRIDSSNFNPLPYWNVGCQLVALNYQSEGRMMQLNEAKFRVNGNCGYVLKPQQMCKGSFNPYSSDPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTIHMPEIALVRFLVWDHDPIGRDFVGQRTLAFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSKQLIGLRGLFNKNPKHSSAESSGHYVRKRSIGDRILRRTASAPAKGRKKSKMGFLEASEIKDSASEPADLKDKEGVVRRTSRSLQARPASMPVDKFLLVGLPCTEDETAQDAKGKDSATANSDGDKTEKETNLKESLFTCSEKTADHSNLASPLKKESAQKETTADFSLVPQEQPEHSVAVSDVTETNRPISNQESLLPAETVPLIPDSAFERITEKIQGKSCAGSRRADDTNRCEEKITPVRTCLPEKVEEVENLKYDIIHRSTVACLSVTDSSPAVYSDAPGLCSSSTMQDSDISRLIDEVSLVTESEMDSAVSALIGQLDVTDDQTSLTAPMLPGAGSRAFSAVATRTLTAELSTPCKKDFIATQSSKSPLFSTPDSAVFPSPETAEYSVYTIIHEATLTPGSEFKTHSGLVCKKSKSAENNLPSSFCSSPFSLLNANPQRKCETSWEAPGSAGSFASSSLIFEEMLVDPPMGENSESSSLVELGGDSQDLLVTACAYKREDVSQLASPLKLRQQQDSRYCGERTFGNCASAGLCAAALDCSDNFRTAGSKLPFPACENVGFLHHRHTDEQKDLMCTSRRSQLDVHSPVLRSALAFPPSPAIKQTSPCKSKSLGDLTSEDISCNFESKYQYISRSFVTSGMRDKKLAAMKTMRPHSTDALTEQLRKLLSLDQDDSQQMLYSRRLDEDCPKALVRKLSSRSQSRVRNIASRAKERQEASKQKSSTAGSIAGVVLRNKPSASPHVVNRHSTGSYIARYLGSFPEEAAEGRGRPEGTCAAWHRGCAERLCAHSPLLQLEPDGDEKPEVYFLLRL

Target ORF information:

RefSeq Version	XM_015291820.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291820.2

ORF Insert Sequence:

ATGAGTGGCT GCAGGAGGAC TCTGCTCCCC ACCTGCCCGC GGCTCGGCCT TGAAAGATGC 
ATGAGTGTTA TGCAGTCTGG GACTCAGATG GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC 
GTTCGTCTCT TCTACCTGGA CGAGCACAGG ACCTGCATCC GATGGAGACC GTCCAGAAAA 
AGTGAAAAGG CAAAAATTGT CATTGATTCT ATTTACAAAG TGACCGAAGG CCGTCAGTCT 
GAAATCTTCC ACCGCCATGC AGAGGGGAGC TTTGACCCGA GCTGTTGCTT TACCATTTAC 
CATGGCAACC ACCTGGAGTC ACTGGATCTG ATTACCTCTA ACCCAGAGGA AGCTCGCACC 
TGGATCACAG GGCTGAAATA TTTGATGGCT GGGATAAGCG ATGAGGACTC GCTTGCTAAG 
CGACAGAGAA CTCATGATCA CTGGGTGAAA CAAACTTTTG AGGAAGCTGA CAAGAATGGA 
GATGGCCTGC TGAACATTGA AGAGATCCAC CAGCTGATGC ATAAGCTAAA TGTTAACCTG 
CCCCGCAGGA AGGTCCGGCA GATGTTTCAG GAAGCTGACA CAGATGAGAA CCAAGGAACT 
CTAAATTTTG AAGAATTTTC AGTGTTTTAC AAGATGATGT CCTTGCGTCG AGACCTGTAC 
TTACTGCTCT TAAGCTACAG TGACAAGAAA GATCACCTCA CCGTTGAAGA ACTTGCTCAA 
TTTCTGAAGG TGGAACAAAA GATGAGTAAC GTGACGCCAG AATATTGTCT TGACATCATA 
CAGAAGTTTG AAGTATCAGA AGAAAACAAG GAGCAGAACG TGCTTGGGAT TGAAGGTTTC 
ACCAACTTCA TGCGCAGTCC TGCTTGTGAT GTTTTTAATC CATTGCATTG CGAAGTGCAT 
CAGGATATGG ATCAACCCCT TTGTAACTAC TTCATTGCTT CATCTCACAA CACGTACCTG 
ACAGGAGACC AGCTGCTGTC CCAGTCTAGG GTGGAGATGT ATGCACGAGT GCTGCAGGAC 
GGTTGTCGAT GTATTGAAGT GGATTGCTGG GATGGTCCAG ATGGAGAGCC AGTAGTGCAT 
CATGGGTATA CGCTTACCTC TAAAATATTC TTCCGTGACG TGGCAGAAAC TATCAATAAG 
TATGCCTTCA TCAAAAATGA GTTTCCGGTG ATACTGTCTA TTGAAAATCA TTGCAGTATT 
CAGCAGCAAA AGAAGATTGC TCAGTACTTG AAGGAGATAC TTGGTGACAA ATTGGACTTG 
TCATCTGTGG TCACCGGAGA TTCCAGGCAG CTTCCTAGCC CTCAAAACTT GAAAGGGAAA 
ATCCTAGTGA AGGGAAAAAA GTTGCCATAC AGTCTTGGAG CAGATGCTGA GGAAGGAGAA 
GTTTCTGATG AGGACAGTGC TGATGAAATT GAAGACGACT GCAAGCTGAA ATCCTGCTAT 
AGTAACGGTG CAACTGAGCA TCAGGTGGAA TCTTTTATAA GAACAAAGCT TGAATCCCTG 
CTAAAGGAGT CCCAGATCAG GGACAAAGAA GATCCTGACA GCTTCACGGT GAGAGCACTG 
CTAAGGGCAA CTCACGAGGG TTTAAGTGTT AACCTGAAGC AGAACCTGGA CACAAAAGAA 
GGTGGGAAGA AGTCTCATAG TCGATCATTA ATGGGCAACT TTGGTAAACA CAAAAAAACA 
GGGAAGGCAA CATCCAAATG TAGCACATCA GATGATGATG AAAATCAGCA AAACTCTTCT 
GGAAAGGAAG CAGGACAGCT GCACAGTAGA CTGACTCGTC GAAGGAAAAC TGTGAAGCTG 
TGCCGAGCTC TGTCTGACCT AGTGGTGTAC ACGAACTCTG TGGCAGCCCA GGATATAGTA 
GATGATGGAT CAACAGGGAA TGTGCTGTCA TTCAGTGAAA CAAGAGCCCA CCAAGCAGTG 
CAGCAGAAGG CTGAACAGTT CATGCTTTAC AACCAGAAAC AGCTTACAAG GGTTTATCCA 
TCAGCGTATC GGATTGACTC CAGCAATTTC AATCCTTTAC CTTACTGGAA TGTTGGTTGC 
CAGCTAGTGG CACTAAACTA CCAGTCTGAG GGACGTATGA TGCAATTAAA CGAAGCAAAA 
TTTAGGGTAA ATGGCAACTG TGGTTACGTC CTCAAACCTC AGCAGATGTG CAAAGGCTCA 
TTCAACCCCT ATTCTTCAGA TCCACTTCCT GCTAGTCCTA AGAAGCAGCT CATCCTGAAA 
ATCATCAGTG GACAGCAGCT GCCTAAGCCT CCTGACTCAA TGCTAGGAGA CAGAGGAGAG 
ATAATAGATC CCTTTGTCGA GGTGGAAATT ATTGGGTTAC CTGTTGACTG CTGTAAAGAT 
CAGACCAGGG TGGTTGATGA CAATGGGTTT AATCCTGTTT GGGAGGAAAC GCTCACTTTT 
ACCATCCATA TGCCTGAAAT AGCTTTGGTT CGATTTCTTG TTTGGGATCA TGACCCCATT 
GGGCGAGACT TCGTTGGACA AAGAACGCTG GCCTTTAGCA GTCTTGTACC TGGTTATCGA 
CATGTGTATT TAGAAGGGCT GACAGAAGCT TCCATATTTG TTCACATAAC CATTAATGAA 
ATCTATGGGA AGTGGAGCCC CTTAATCCTC AATCCCAGTT ACACAATATT GCACTTTCTA 
GGAGCCACAA AGAGTAAGCA ACTGATCGGA CTCCGAGGGC TGTTTAACAA GAACCCAAAG 
CACAGTTCTG CTGAAAGCAG CGGTCATTAT GTACGGAAGC GTTCTATTGG TGACCGAATT 
CTCCGGCGCA CGGCCAGTGC TCCAGCAAAA GGTAGAAAGA AAAGCAAGAT GGGTTTTCTG 
GAAGCTTCTG AAATTAAAGA CAGTGCATCC GAACCAGCAG ACCTGAAGGA CAAAGAAGGA 
GTTGTAAGGC GTACAAGCCG GAGTTTGCAA GCACGTCCTG CTTCTATGCC AGTGGACAAA 
TTCCTTCTTG TAGGACTGCC GTGCACAGAA GATGAAACTG CTCAAGATGC CAAAGGAAAA 
GACAGTGCAA CTGCCAACAG TGATGGCGAT AAAACTGAGA AGGAAACAAA CTTGAAGGAA 
TCTCTTTTCA CGTGTTCTGA GAAAACTGCA GATCATTCAA ACTTGGCATC TCCCTTGAAA 
AAGGAGAGCG CCCAGAAGGA AACTACAGCT GATTTTTCTC TTGTACCCCA GGAGCAGCCC 
GAGCATTCGG TTGCTGTAAG TGATGTAACC GAAACAAACC GCCCCATAAG CAATCAGGAA 
AGTCTCCTTC CTGCTGAAAC GGTGCCTCTG ATTCCGGACT CTGCCTTTGA GCGCATCACA 
GAAAAAATAC AGGGCAAGAG CTGTGCAGGC AGTAGAAGGG CAGATGACAC AAACAGGTGT 
GAGGAGAAAA TAACTCCTGT CAGGACTTGC CTTCCTGAAA AAGTAGAGGA GGTTGAAAAT 
CTCAAATACG ACATCATCCA CAGAAGCACT GTGGCGTGTC TTTCTGTGAC AGATAGTTCT 
CCTGCCGTCT ACTCTGATGC TCCTGGTTTA TGCTCCTCTT CAACCATGCA AGACAGCGAC 
ATCTCCCGCC TCATAGACGA AGTTTCTTTG GTGACTGAGA GCGAGATGGA CAGCGCTGTC 
TCAGCTCTGA TCGGCCAGCT GGATGTCACC GATGACCAAA CCAGCCTCAC CGCACCGATG 
CTCCCTGGCG CTGGCAGCCG GGCCTTCAGT GCTGTGGCCA CACGCACACT GACTGCAGAG 
CTCAGCACTC CCTGTAAGAA GGACTTCATT GCCACACAGT CCAGCAAGTC CCCGTTATTT 
TCAACTCCAG ACTCTGCCGT GTTCCCCAGC CCCGAGACTG CGGAGTATTC TGTGTACACA 
ATTATCCACG AGGCAACTCT TACACCCGGT TCTGAATTTA AGACCCACAG TGGGTTAGTT 
TGCAAGAAGT CAAAGAGTGC AGAAAATAAC TTGCCTAGCT CTTTTTGTTC TTCACCTTTC 
TCTTTGCTGA ATGCAAATCC CCAAAGAAAA TGTGAAACAA GCTGGGAAGC CCCCGGCTCT 
GCGGGTTCTT TTGCTTCCAG TAGCCTCATC TTTGAGGAGA TGCTTGTGGA CCCTCCCATG 
GGCGAGAATT CGGAAAGCAG CAGTCTTGTA GAGTTAGGTG GGGATTCCCA AGACCTCTTG 
GTAACTGCGT GCGCGTACAA AAGGGAAGAT GTGAGCCAAT TGGCTTCCCC TTTAAAACTG 
AGGCAGCAGC AGGACAGCAG GTACTGTGGT GAGAGGACCT TTGGAAACTG TGCAAGTGCT 
GGGCTCTGTG CTGCTGCCCT GGACTGCTCG GATAACTTTA GGACTGCGGG AAGCAAGCTC 
CCTTTTCCAG CCTGTGAAAA TGTTGGTTTT CTACATCATC GTCACACAGA TGAGCAGAAA 
GATTTGATGT GTACCTCCAG GAGGTCCCAA CTCGATGTGC ACTCACCAGT GCTCAGAAGC 
GCGTTGGCTT TCCCACCCTC CCCAGCCATC AAACAGACCA GCCCTTGCAA GTCCAAGAGC 
CTTGGTGACT TGACATCGGA AGATATTTCC TGCAATTTTG AAAGTAAGTA TCAGTACATA 
AGTAGGAGCT TTGTCACATC TGGCATGAGA GACAAGAAGC TCGCTGCCAT GAAGACGATG 
AGACCGCACT CTACGGATGC TTTGACGGAA CAGCTGAGAA AGCTGCTGTC CCTGGACCAG 
GACGACAGCC AGCAGATGCT GTATTCCAGG CGGCTCGACG AGGACTGCCC AAAGGCACTG 
GTCAGAAAGC TGTCGTCCAG GAGCCAGAGC AGAGTGCGGA ACATCGCCAG CCGTGCCAAG 
GAGAGGCAGG AGGCCAGCAA GCAAAAATCT TCCACCGCAG GCAGCATAGC GGGGGTCGTC 
CTTCGGAACA AGCCTTCGGC GTCCCCTCAC GTCGTCAACA GGCACTCGAC GGGCTCCTAC 
ATAGCCAGGT ACCTGGGCAG CTTCCCTGAG GAGGCAGCAG AGGGCCGGGG CCGACCTGAG 
GGCACGTGTG CTGCCTGGCA CCGCGGCTGT GCTGAGCGGC TGTGTGCGCA CAGCCCCCTG 
CTGCAGCTGG AGCCCGACGG CGACGAGAAG CCTGAAGTCT ATTTCCTGCT GAGGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_015291820.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147306.1 Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X5, mRNA.

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CloneID	OGa40160
Clone ID Related Accession (Same CDS sequence)	XM_015291820.2 , XM_015291820.1
Accession Version	XM_015291820.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCT GCAGGAGGAC TCTGCTCCCC ACCTGCCCGC GGCTCGGCCT TGAAAGATGC 
ATGAGTGTTA TGCAGTCTGG GACTCAGATG GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC 
GTTCGTCTCT TCTACCTGGA CGAGCACAGG ACCTGCATCC GATGGAGACC GTCCAGAAAA 
AGTGAAAAGG CAAAAATTGT CATTGATTCT ATTTACAAAG TGACCGAAGG CCGTCAGTCT 
GAAATCTTCC ACCGCCATGC AGAGGGGAGC TTTGACCCGA GCTGTTGCTT TACCATTTAC 
CATGGCAACC ACCTGGAGTC ACTGGATCTG ATTACCTCTA ACCCAGAGGA AGCTCGCACC 
TGGATCACAG GGCTGAAATA TTTGATGGCT GGGATAAGCG ATGAGGACTC GCTTGCTAAG 
CGACAGAGAA CTCATGATCA CTGGGTGAAA CAAACTTTTG AGGAAGCTGA CAAGAATGGA 
GATGGCCTGC TGAACATTGA AGAGATCCAC CAGCTGATGC ATAAGCTAAA TGTTAACCTG 
CCCCGCAGGA AGGTCCGGCA GATGTTTCAG GAAGCTGACA CAGATGAGAA CCAAGGAACT 
CTAAATTTTG AAGAATTTTC AGTGTTTTAC AAGATGATGT CCTTGCGTCG AGACCTGTAC 
TTACTGCTCT TAAGCTACAG TGACAAGAAA GATCACCTCA CCGTTGAAGA ACTTGCTCAA 
TTTCTGAAGG TGGAACAAAA GATGAGTAAC GTGACGCCAG AATATTGTCT TGACATCATA 
CAGAAGTTTG AAGTATCAGA AGAAAACAAG GAGCAGAACG TGCTTGGGAT TGAAGGTTTC 
ACCAACTTCA TGCGCAGTCC TGCTTGTGAT GTTTTTAATC CATTGCATTG CGAAGTGCAT 
CAGGATATGG ATCAACCCCT TTGTAACTAC TTCATTGCTT CATCTCACAA CACGTACCTG 
ACAGGAGACC AGCTGCTGTC CCAGTCTAGG GTGGAGATGT ATGCACGAGT GCTGCAGGAC 
GGTTGTCGAT GTATTGAAGT GGATTGCTGG GATGGTCCAG ATGGAGAGCC AGTAGTGCAT 
CATGGGTATA CGCTTACCTC TAAAATATTC TTCCGTGACG TGGCAGAAAC TATCAATAAG 
TATGCCTTCA TCAAAAATGA GTTTCCGGTG ATACTGTCTA TTGAAAATCA TTGCAGTATT 
CAGCAGCAAA AGAAGATTGC TCAGTACTTG AAGGAGATAC TTGGTGACAA ATTGGACTTG 
TCATCTGTGG TCACCGGAGA TTCCAGGCAG CTTCCTAGCC CTCAAAACTT GAAAGGGAAA 
ATCCTAGTGA AGGGAAAAAA GTTGCCATAC AGTCTTGGAG CAGATGCTGA GGAAGGAGAA 
GTTTCTGATG AGGACAGTGC TGATGAAATT GAAGACGACT GCAAGCTGAA ATCCTGCTAT 
AGTAACGGTG CAACTGAGCA TCAGGTGGAA TCTTTTATAA GAACAAAGCT TGAATCCCTG 
CTAAAGGAGT CCCAGATCAG GGACAAAGAA GATCCTGACA GCTTCACGGT GAGAGCACTG 
CTAAGGGCAA CTCACGAGGG TTTAAGTGTT AACCTGAAGC AGAACCTGGA CACAAAAGAA 
GGTGGGAAGA AGTCTCATAG TCGATCATTA ATGGGCAACT TTGGTAAACA CAAAAAAACA 
GGGAAGGCAA CATCCAAATG TAGCACATCA GATGATGATG AAAATCAGCA AAACTCTTCT 
GGAAAGGAAG CAGGACAGCT GCACAGTAGA CTGACTCGTC GAAGGAAAAC TGTGAAGCTG 
TGCCGAGCTC TGTCTGACCT AGTGGTGTAC ACGAACTCTG TGGCAGCCCA GGATATAGTA 
GATGATGGAT CAACAGGGAA TGTGCTGTCA TTCAGTGAAA CAAGAGCCCA CCAAGCAGTG 
CAGCAGAAGG CTGAACAGTT CATGCTTTAC AACCAGAAAC AGCTTACAAG GGTTTATCCA 
TCAGCGTATC GGATTGACTC CAGCAATTTC AATCCTTTAC CTTACTGGAA TGTTGGTTGC 
CAGCTAGTGG CACTAAACTA CCAGTCTGAG GGACGTATGA TGCAATTAAA CGAAGCAAAA 
TTTAGGGTAA ATGGCAACTG TGGTTACGTC CTCAAACCTC AGCAGATGTG CAAAGGCTCA 
TTCAACCCCT ATTCTTCAGA TCCACTTCCT GCTAGTCCTA AGAAGCAGCT CATCCTGAAA 
ATCATCAGTG GACAGCAGCT GCCTAAGCCT CCTGACTCAA TGCTAGGAGA CAGAGGAGAG 
ATAATAGATC CCTTTGTCGA GGTGGAAATT ATTGGGTTAC CTGTTGACTG CTGTAAAGAT 
CAGACCAGGG TGGTTGATGA CAATGGGTTT AATCCTGTTT GGGAGGAAAC GCTCACTTTT 
ACCATCCATA TGCCTGAAAT AGCTTTGGTT CGATTTCTTG TTTGGGATCA TGACCCCATT 
GGGCGAGACT TCGTTGGACA AAGAACGCTG GCCTTTAGCA GTCTTGTACC TGGTTATCGA 
CATGTGTATT TAGAAGGGCT GACAGAAGCT TCCATATTTG TTCACATAAC CATTAATGAA 
ATCTATGGGA AGTGGAGCCC CTTAATCCTC AATCCCAGTT ACACAATATT GCACTTTCTA 
GGAGCCACAA AGAGTAAGCA ACTGATCGGA CTCCGAGGGC TGTTTAACAA GAACCCAAAG 
CACAGTTCTG CTGAAAGCAG CGGTCATTAT GTACGGAAGC GTTCTATTGG TGACCGAATT 
CTCCGGCGCA CGGCCAGTGC TCCAGCAAAA GGTAGAAAGA AAAGCAAGAT GGGTTTTCTG 
GAAGCTTCTG AAATTAAAGA CAGTGCATCC GAACCAGCAG ACCTGAAGGA CAAAGAAGGA 
GTTGTAAGGC GTACAAGCCG GAGTTTGCAA GCACGTCCTG CTTCTATGCC AGTGGACAAA 
TTCCTTCTTG TAGGACTGCC GTGCACAGAA GATGAAACTG CTCAAGATGC CAAAGGAAAA 
GACAGTGCAA CTGCCAACAG TGATGGCGAT AAAACTGAGA AGGAAACAAA CTTGAAGGAA 
TCTCTTTTCA CGTGTTCTGA GAAAACTGCA GATCATTCAA ACTTGGCATC TCCCTTGAAA 
AAGGAGAGCG CCCAGAAGGA AACTACAGCT GATTTTTCTC TTGTACCCCA GGAGCAGCCC 
GAGCATTCGG TTGCTGTAAG TGATGTAACC GAAACAAACC GCCCCATAAG CAATCAGGAA 
AGTCTCCTTC CTGCTGAAAC GGTGCCTCTG ATTCCGGACT CTGCCTTTGA GCGCATCACA 
GAAAAAATAC AGGGCAAGAG CTGTGCAGGC AGTAGAAGGG CAGATGACAC AAACAGGTGT 
GAGGAGAAAA TAACTCCTGT CAGGACTTGC CTTCCTGAAA AAGTAGAGGA GGTTGAAAAT 
CTCAAATACG ACATCATCCA CAGAAGCACT GTGGCGTGTC TTTCTGTGAC AGATAGTTCT 
CCTGCCGTCT ACTCTGATGC TCCTGGTTTA TGCTCCTCTT CAACCATGCA AGACAGCGAC 
ATCTCCCGCC TCATAGACGA AGTTTCTTTG GTGACTGAGA GCGAGATGGA CAGCGCTGTC 
TCAGCTCTGA TCGGCCAGCT GGATGTCACC GATGACCAAA CCAGCCTCAC CGCACCGATG 
CTCCCTGGCG CTGGCAGCCG GGCCTTCAGT GCTGTGGCCA CACGCACACT GACTGCAGAG 
CTCAGCACTC CCTGTAAGAA GGACTTCATT GCCACACAGT CCAGCAAGTC CCCGTTATTT 
TCAACTCCAG ACTCTGCCGT GTTCCCCAGC CCCGAGACTG CGGAGTATTC TGTGTACACA 
ATTATCCACG AGGCAACTCT TACACCCGGT TCTGAATTTA AGACCCACAG TGGGTTAGTT 
TGCAAGAAGT CAAAGAGTGC AGAAAATAAC TTGCCTAGCT CTTTTTGTTC TTCACCTTTC 
TCTTTGCTGA ATGCAAATCC CCAAAGAAAA TGTGAAACAA GCTGGGAAGC CCCCGGCTCT 
GCGGGTTCTT TTGCTTCCAG TAGCCTCATC TTTGAGGAGA TGCTTGTGGA CCCTCCCATG 
GGCGAGAATT CGGAAAGCAG CAGTCTTGTA GAGTTAGGTG GGGATTCCCA AGACCTCTTG 
GTAACTGCGT GCGCGTACAA AAGGGAAGAT GTGAGCCAAT TGGCTTCCCC TTTAAAACTG 
AGGCAGCAGC AGGACAGCAG GTACTGTGGT GAGAGGACCT TTGGAAACTG TGCAAGTGCT 
GGGCTCTGTG CTGCTGCCCT GGACTGCTCG GATAACTTTA GGACTGCGGG AAGCAAGCTC 
CCTTTTCCAG CCTGTGAAAA TGTTGGTTTT CTACATCATC GTCACACAGA TGAGCAGAAA 
GATTTGATGT GTACCTCCAG GAGGTCCCAA CTCGATGTGC ACTCACCAGT GCTCAGAAGC 
GCGTTGGCTT TCCCACCCTC CCCAGCCATC AAACAGACCA GCCCTTGCAA GTCCAAGAGC 
CTTGGTGACT TGACATCGGA AGATATTTCC TGCAATTTTG AAAGTAAGTA TCAGTACATA 
AGTAGGAGCT TTGTCACATC TGGCATGAGA GACAAGAAGC TCGCTGCCAT GAAGACGATG 
AGACCGCACT CTACGGATGC TTTGACGGAA CAGCTGAGAA AGCTGCTGTC CCTGGACCAG 
GACGACAGCC AGCAGATGCT GTATTCCAGG CGGCTCGACG AGGACTGCCC AAAGGCACTG 
GTCAGAAAGC TGTCGTCCAG GAGCCAGAGC AGAGTGCGGA ACATCGCCAG CCGTGCCAAG 
GAGAGGCAGG AGGCCAGCAA GCAAAAATCT TCCACCGCAG GCAGCATAGC GGGGGTCGTC 
CTTCGGAACA AGCCTTCGGC GTCCCCTCAC GTCGTCAACA GGCACTCGAC GGGCTCCTAC 
ATAGCCAGGT ACCTGGGCAG CTTCCCTGAG GAGGCAGCAG AGGGCCGGGG CCGACCTGAG 
GGCACGTGTG CTGCCTGGCA CCGCGGCTGT GCTGAGCGGC TGTGTGCGCA CAGCCCCCTG 
CTGCAGCTGG AGCCCGACGG CGACGAGAAG CCTGAAGTCT ATTTCCTGCT GAGGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147306.1
CDS	263..5362
Translation	MSGCRRTLLPTCPRLGLERCMSVMQSGTQMVKLKSGTKGLVRLFYLDEHRTCIRWRPSRKSEKAKIVIDSIYKVTEGRQSEIFHRHAEGSFDPSCCFTIYHGNHLESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDHWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLNFEEFSVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTPEYCLDIIQKFEVSEENKEQNVLGIEGFTNFMRSPACDVFNPLHCEVHQDMDQPLCNYFIASSHNTYLTGDQLLSQSRVEMYARVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKIFFRDVAETINKYAFIKNEFPVILSIENHCSIQQQKKIAQYLKEILGDKLDLSSVVTGDSRQLPSPQNLKGKILVKGKKLPYSLGADAEEGEVSDEDSADEIEDDCKLKSCYSNGATEHQVESFIRTKLESLLKESQIRDKEDPDSFTVRALLRATHEGLSVNLKQNLDTKEGGKKSHSRSLMGNFGKHKKTGKATSKCSTSDDDENQQNSSGKEAGQLHSRLTRRRKTVKLCRALSDLVVYTNSVAAQDIVDDGSTGNVLSFSETRAHQAVQQKAEQFMLYNQKQLTRVYPSAYRIDSSNFNPLPYWNVGCQLVALNYQSEGRMMQLNEAKFRVNGNCGYVLKPQQMCKGSFNPYSSDPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTIHMPEIALVRFLVWDHDPIGRDFVGQRTLAFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSKQLIGLRGLFNKNPKHSSAESSGHYVRKRSIGDRILRRTASAPAKGRKKSKMGFLEASEIKDSASEPADLKDKEGVVRRTSRSLQARPASMPVDKFLLVGLPCTEDETAQDAKGKDSATANSDGDKTEKETNLKESLFTCSEKTADHSNLASPLKKESAQKETTADFSLVPQEQPEHSVAVSDVTETNRPISNQESLLPAETVPLIPDSAFERITEKIQGKSCAGSRRADDTNRCEEKITPVRTCLPEKVEEVENLKYDIIHRSTVACLSVTDSSPAVYSDAPGLCSSSTMQDSDISRLIDEVSLVTESEMDSAVSALIGQLDVTDDQTSLTAPMLPGAGSRAFSAVATRTLTAELSTPCKKDFIATQSSKSPLFSTPDSAVFPSPETAEYSVYTIIHEATLTPGSEFKTHSGLVCKKSKSAENNLPSSFCSSPFSLLNANPQRKCETSWEAPGSAGSFASSSLIFEEMLVDPPMGENSESSSLVELGGDSQDLLVTACAYKREDVSQLASPLKLRQQQDSRYCGERTFGNCASAGLCAAALDCSDNFRTAGSKLPFPACENVGFLHHRHTDEQKDLMCTSRRSQLDVHSPVLRSALAFPPSPAIKQTSPCKSKSLGDLTSEDISCNFESKYQYISRSFVTSGMRDKKLAAMKTMRPHSTDALTEQLRKLLSLDQDDSQQMLYSRRLDEDCPKALVRKLSSRSQSRVRNIASRAKERQEASKQKSSTAGSIAGVVLRNKPSASPHVVNRHSTGSYIARYLGSFPEEAAEGRGRPEGTCAAWHRGCAERLCAHSPLLQLEPDGDEKPEVYFLLRL

Target ORF information:

RefSeq Version	XM_015291820.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291820.1

ORF Insert Sequence:

ATGAGTGGCT GCAGGAGGAC TCTGCTCCCC ACCTGCCCGC GGCTCGGCCT TGAAAGATGC 
ATGAGTGTTA TGCAGTCTGG GACTCAGATG GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC 
GTTCGTCTCT TCTACCTGGA CGAGCACAGG ACCTGCATCC GATGGAGACC GTCCAGAAAA 
AGTGAAAAGG CAAAAATTGT CATTGATTCT ATTTACAAAG TGACCGAAGG CCGTCAGTCT 
GAAATCTTCC ACCGCCATGC AGAGGGGAGC TTTGACCCGA GCTGTTGCTT TACCATTTAC 
CATGGCAACC ACCTGGAGTC ACTGGATCTG ATTACCTCTA ACCCAGAGGA AGCTCGCACC 
TGGATCACAG GGCTGAAATA TTTGATGGCT GGGATAAGCG ATGAGGACTC GCTTGCTAAG 
CGACAGAGAA CTCATGATCA CTGGGTGAAA CAAACTTTTG AGGAAGCTGA CAAGAATGGA 
GATGGCCTGC TGAACATTGA AGAGATCCAC CAGCTGATGC ATAAGCTAAA TGTTAACCTG 
CCCCGCAGGA AGGTCCGGCA GATGTTTCAG GAAGCTGACA CAGATGAGAA CCAAGGAACT 
CTAAATTTTG AAGAATTTTC AGTGTTTTAC AAGATGATGT CCTTGCGTCG AGACCTGTAC 
TTACTGCTCT TAAGCTACAG TGACAAGAAA GATCACCTCA CCGTTGAAGA ACTTGCTCAA 
TTTCTGAAGG TGGAACAAAA GATGAGTAAC GTGACGCCAG AATATTGTCT TGACATCATA 
CAGAAGTTTG AAGTATCAGA AGAAAACAAG GAGCAGAACG TGCTTGGGAT TGAAGGTTTC 
ACCAACTTCA TGCGCAGTCC TGCTTGTGAT GTTTTTAATC CATTGCATTG CGAAGTGCAT 
CAGGATATGG ATCAACCCCT TTGTAACTAC TTCATTGCTT CATCTCACAA CACGTACCTG 
ACAGGAGACC AGCTGCTGTC CCAGTCTAGG GTGGAGATGT ATGCACGAGT GCTGCAGGAC 
GGTTGTCGAT GTATTGAAGT GGATTGCTGG GATGGTCCAG ATGGAGAGCC AGTAGTGCAT 
CATGGGTATA CGCTTACCTC TAAAATATTC TTCCGTGACG TGGCAGAAAC TATCAATAAG 
TATGCCTTCA TCAAAAATGA GTTTCCGGTG ATACTGTCTA TTGAAAATCA TTGCAGTATT 
CAGCAGCAAA AGAAGATTGC TCAGTACTTG AAGGAGATAC TTGGTGACAA ATTGGACTTG 
TCATCTGTGG TCACCGGAGA TTCCAGGCAG CTTCCTAGCC CTCAAAACTT GAAAGGGAAA 
ATCCTAGTGA AGGGAAAAAA GTTGCCATAC AGTCTTGGAG CAGATGCTGA GGAAGGAGAA 
GTTTCTGATG AGGACAGTGC TGATGAAATT GAAGACGACT GCAAGCTGAA ATCCTGCTAT 
AGTAACGGTG CAACTGAGCA TCAGGTGGAA TCTTTTATAA GAACAAAGCT TGAATCCCTG 
CTAAAGGAGT CCCAGATCAG GGACAAAGAA GATCCTGACA GCTTCACGGT GAGAGCACTG 
CTAAGGGCAA CTCACGAGGG TTTAAGTGTT AACCTGAAGC AGAACCTGGA CACAAAAGAA 
GGTGGGAAGA AGTCTCATAG TCGATCATTA ATGGGCAACT TTGGTAAACA CAAAAAAACA 
GGGAAGGCAA CATCCAAATG TAGCACATCA GATGATGATG AAAATCAGCA AAACTCTTCT 
GGAAAGGAAG CAGGACAGCT GCACAGTAGA CTGACTCGTC GAAGGAAAAC TGTGAAGCTG 
TGCCGAGCTC TGTCTGACCT AGTGGTGTAC ACGAACTCTG TGGCAGCCCA GGATATAGTA 
GATGATGGAT CAACAGGGAA TGTGCTGTCA TTCAGTGAAA CAAGAGCCCA CCAAGCAGTG 
CAGCAGAAGG CTGAACAGTT CATGCTTTAC AACCAGAAAC AGCTTACAAG GGTTTATCCA 
TCAGCGTATC GGATTGACTC CAGCAATTTC AATCCTTTAC CTTACTGGAA TGTTGGTTGC 
CAGCTAGTGG CACTAAACTA CCAGTCTGAG GGACGTATGA TGCAATTAAA CGAAGCAAAA 
TTTAGGGTAA ATGGCAACTG TGGTTACGTC CTCAAACCTC AGCAGATGTG CAAAGGCTCA 
TTCAACCCCT ATTCTTCAGA TCCACTTCCT GCTAGTCCTA AGAAGCAGCT CATCCTGAAA 
ATCATCAGTG GACAGCAGCT GCCTAAGCCT CCTGACTCAA TGCTAGGAGA CAGAGGAGAG 
ATAATAGATC CCTTTGTCGA GGTGGAAATT ATTGGGTTAC CTGTTGACTG CTGTAAAGAT 
CAGACCAGGG TGGTTGATGA CAATGGGTTT AATCCTGTTT GGGAGGAAAC GCTCACTTTT 
ACCATCCATA TGCCTGAAAT AGCTTTGGTT CGATTTCTTG TTTGGGATCA TGACCCCATT 
GGGCGAGACT TCGTTGGACA AAGAACGCTG GCCTTTAGCA GTCTTGTACC TGGTTATCGA 
CATGTGTATT TAGAAGGGCT GACAGAAGCT TCCATATTTG TTCACATAAC CATTAATGAA 
ATCTATGGGA AGTGGAGCCC CTTAATCCTC AATCCCAGTT ACACAATATT GCACTTTCTA 
GGAGCCACAA AGAGTAAGCA ACTGATCGGA CTCCGAGGGC TGTTTAACAA GAACCCAAAG 
CACAGTTCTG CTGAAAGCAG CGGTCATTAT GTACGGAAGC GTTCTATTGG TGACCGAATT 
CTCCGGCGCA CGGCCAGTGC TCCAGCAAAA GGTAGAAAGA AAAGCAAGAT GGGTTTTCTG 
GAAGCTTCTG AAATTAAAGA CAGTGCATCC GAACCAGCAG ACCTGAAGGA CAAAGAAGGA 
GTTGTAAGGC GTACAAGCCG GAGTTTGCAA GCACGTCCTG CTTCTATGCC AGTGGACAAA 
TTCCTTCTTG TAGGACTGCC GTGCACAGAA GATGAAACTG CTCAAGATGC CAAAGGAAAA 
GACAGTGCAA CTGCCAACAG TGATGGCGAT AAAACTGAGA AGGAAACAAA CTTGAAGGAA 
TCTCTTTTCA CGTGTTCTGA GAAAACTGCA GATCATTCAA ACTTGGCATC TCCCTTGAAA 
AAGGAGAGCG CCCAGAAGGA AACTACAGCT GATTTTTCTC TTGTACCCCA GGAGCAGCCC 
GAGCATTCGG TTGCTGTAAG TGATGTAACC GAAACAAACC GCCCCATAAG CAATCAGGAA 
AGTCTCCTTC CTGCTGAAAC GGTGCCTCTG ATTCCGGACT CTGCCTTTGA GCGCATCACA 
GAAAAAATAC AGGGCAAGAG CTGTGCAGGC AGTAGAAGGG CAGATGACAC AAACAGGTGT 
GAGGAGAAAA TAACTCCTGT CAGGACTTGC CTTCCTGAAA AAGTAGAGGA GGTTGAAAAT 
CTCAAATACG ACATCATCCA CAGAAGCACT GTGGCGTGTC TTTCTGTGAC AGATAGTTCT 
CCTGCCGTCT ACTCTGATGC TCCTGGTTTA TGCTCCTCTT CAACCATGCA AGACAGCGAC 
ATCTCCCGCC TCATAGACGA AGTTTCTTTG GTGACTGAGA GCGAGATGGA CAGCGCTGTC 
TCAGCTCTGA TCGGCCAGCT GGATGTCACC GATGACCAAA CCAGCCTCAC CGCACCGATG 
CTCCCTGGCG CTGGCAGCCG GGCCTTCAGT GCTGTGGCCA CACGCACACT GACTGCAGAG 
CTCAGCACTC CCTGTAAGAA GGACTTCATT GCCACACAGT CCAGCAAGTC CCCGTTATTT 
TCAACTCCAG ACTCTGCCGT GTTCCCCAGC CCCGAGACTG CGGAGTATTC TGTGTACACA 
ATTATCCACG AGGCAACTCT TACACCCGGT TCTGAATTTA AGACCCACAG TGGGTTAGTT 
TGCAAGAAGT CAAAGAGTGC AGAAAATAAC TTGCCTAGCT CTTTTTGTTC TTCACCTTTC 
TCTTTGCTGA ATGCAAATCC CCAAAGAAAA TGTGAAACAA GCTGGGAAGC CCCCGGCTCT 
GCGGGTTCTT TTGCTTCCAG TAGCCTCATC TTTGAGGAGA TGCTTGTGGA CCCTCCCATG 
GGCGAGAATT CGGAAAGCAG CAGTCTTGTA GAGTTAGGTG GGGATTCCCA AGACCTCTTG 
GTAACTGCGT GCGCGTACAA AAGGGAAGAT GTGAGCCAAT TGGCTTCCCC TTTAAAACTG 
AGGCAGCAGC AGGACAGCAG GTACTGTGGT GAGAGGACCT TTGGAAACTG TGCAAGTGCT 
GGGCTCTGTG CTGCTGCCCT GGACTGCTCG GATAACTTTA GGACTGCGGG AAGCAAGCTC 
CCTTTTCCAG CCTGTGAAAA TGTTGGTTTT CTACATCATC GTCACACAGA TGAGCAGAAA 
GATTTGATGT GTACCTCCAG GAGGTCCCAA CTCGATGTGC ACTCACCAGT GCTCAGAAGC 
GCGTTGGCTT TCCCACCCTC CCCAGCCATC AAACAGACCA GCCCTTGCAA GTCCAAGAGC 
CTTGGTGACT TGACATCGGA AGATATTTCC TGCAATTTTG AAAGTAAGTA TCAGTACATA 
AGTAGGAGCT TTGTCACATC TGGCATGAGA GACAAGAAGC TCGCTGCCAT GAAGACGATG 
AGACCGCACT CTACGGATGC TTTGACGGAA CAGCTGAGAA AGCTGCTGTC CCTGGACCAG 
GACGACAGCC AGCAGATGCT GTATTCCAGG CGGCTCGACG AGGACTGCCC AAAGGCACTG 
GTCAGAAAGC TGTCGTCCAG GAGCCAGAGC AGAGTGCGGA ACATCGCCAG CCGTGCCAAG 
GAGAGGCAGG AGGCCAGCAA GCAAAAATCT TCCACCGCAG GCAGCATAGC GGGGGTCGTC 
CTTCGGAACA AGCCTTCGGC GTCCCCTCAC GTCGTCAACA GGCACTCGAC GGGCTCCTAC 
ATAGCCAGGT ACCTGGGCAG CTTCCCTGAG GAGGCAGCAG AGGGCCGGGG CCGACCTGAG 
GGCACGTGTG CTGCCTGGCA CCGCGGCTGT GCTGAGCGGC TGTGTGCGCA CAGCCCCCTG 
CTGCAGCTGG AGCCCGACGG CGACGAGAAG CCTGAAGTCT ATTTCCTGCT GAGGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_015291821.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147307.1 Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X6, mRNA.

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CloneID	OGa40161
Clone ID Related Accession (Same CDS sequence)	XM_015291821.1 , XM_015291821.2
Accession Version	XM_015291821.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACC TTGAAGTATA TAAAAATTTA AGTCCTGAAA AAGTTGAAAG ATGCATGAGT 
GTTATGCAGT CTGGGACTCA GATGGTTAAG CTGAAGAGTG GAACCAAAGG GCTCGTTCGT 
CTCTTCTACC TGGACGAGCA CAGGACCTGC ATCCGATGGA GACCGTCCAG AAAAAGTGAA 
AAGGCAAAAA TTGTCATTGA TTCTATTTAC AAAGTGACCG AAGGCCGTCA GTCTGAAATC 
TTCCACCGCC ATGCAGAGGG GAGCTTTGAC CCGAGCTGTT GCTTTACCAT TTACCATGGC 
AACCACCTGG AGTCACTGGA TCTGATTACC TCTAACCCAG AGGAAGCTCG CACCTGGATC 
ACAGGGCTGA AATATTTGAT GGCTGGGATA AGCGATGAGG ACTCGCTTGC TAAGCGACAG 
AGAACTCATG ATCACTGGGT GAAACAAACT TTTGAGGAAG CTGACAAGAA TGGAGATGGC 
CTGCTGAACA TTGAAGAGAT CCACCAGCTG ATGCATAAGC TAAATGTTAA CCTGCCCCGC 
AGGAAGGTCC GGCAGATGTT TCAGGAAGCT GACACAGATG AGAACCAAGG AACTCTAAAT 
TTTGAAGAAT TTTCAGTGTT TTACAAGATG ATGTCCTTGC GTCGAGACCT GTACTTACTG 
CTCTTAAGCT ACAGTGACAA GAAAGATCAC CTCACCGTTG AAGAACTTGC TCAATTTCTG 
AAGGTGGAAC AAAAGATGAG TAACGTGACG CCAGAATATT GTCTTGACAT CATACAGAAG 
TTTGAAGTAT CAGAAGAAAA CAAGGAGCAG AACGTGCTTG GGATTGAAGG TTTCACCAAC 
TTCATGCGCA GTCCTGCTTG TGATGTTTTT AATCCATTGC ATTGCGAAGT GCATCAGGAT 
ATGGATCAAC CCCTTTGTAA CTACTTCATT GCTTCATCTC ACAACACGTA CCTGACAGGA 
GACCAGCTGC TGTCCCAGTC TAGGGTGGAG ATGTATGCAC GAGTGCTGCA GGACGGTTGT 
CGATGTATTG AAGTGGATTG CTGGGATGGT CCAGATGGAG AGCCAGTAGT GCATCATGGG 
TATACGCTTA CCTCTAAAAT ATTCTTCCGT GACGTGGCAG AAACTATCAA TAAGTATGCC 
TTCATCAAAA ATGAGTTTCC GGTGATACTG TCTATTGAAA ATCATTGCAG TATTCAGCAG 
CAAAAGAAGA TTGCTCAGTA CTTGAAGGAG ATACTTGGTG ACAAATTGGA CTTGTCATCT 
GTGGTCACCG GAGATTCCAG GCAGCTTCCT AGCCCTCAAA ACTTGAAAGG GAAAATCCTA 
GTGAAGGGAA AAAAGTTGCC ATACAGTCTT GGAGCAGATG CTGAGGAAGG AGAAGTTTCT 
GATGAGGACA GTGCTGATGA AATTGAAGAC GACTGCAAGC TGAAATCCTG CTATAGTAAC 
GGTGCAACTG AGCATCAGGT GGAATCTTTT ATAAGAACAA AGCTTGAATC CCTGCTAAAG 
GAGTCCCAGA TCAGGGACAA AGAAGATCCT GACAGCTTCA CGGTGAGAGC ACTGCTAAGG 
GCAACTCACG AGGGTTTAAG TGTTAACCTG AAGCAGAACC TGGACACAAA AGAAGGTGGG 
AAGAAGTCTC ATAGTCGATC ATTAATGGGC AACTTTGGTA AACACAAAAA AACAGGGAAG 
GCAACATCCA AATGTAGCAC ATCAGATGAT GATGAAAATC AGCAAAACTC TTCTGGAAAG 
GAAGCAGGAC AGCTGCACAG TAGACTGACT CGTCGAAGGA AAACTGTGAA GCTGTGCCGA 
GCTCTGTCTG ACCTAGTGGT GTACACGAAC TCTGTGGCAG CCCAGGATAT AGTAGATGAT 
GGATCAACAG GGAATGTGCT GTCATTCAGT GAAACAAGAG CCCACCAAGC AGTGCAGCAG 
AAGGCTGAAC AGTTCATGCT TTACAACCAG AAACAGCTTA CAAGGGTTTA TCCATCAGCG 
TATCGGATTG ACTCCAGCAA TTTCAATCCT TTACCTTACT GGAATGTTGG TTGCCAGCTA 
GTGGCACTAA ACTACCAGTC TGAGGGACGT ATGATGCAAT TAAACGAAGC AAAATTTAGG 
GTAAATGGCA ACTGTGGTTA CGTCCTCAAA CCTCAGCAGA TGTGCAAAGG CTCATTCAAC 
CCCTATTCTT CAGATCCACT TCCTGCTAGT CCTAAGAAGC AGCTCATCCT GAAAATCATC 
AGTGGACAGC AGCTGCCTAA GCCTCCTGAC TCAATGCTAG GAGACAGAGG AGAGATAATA 
GATCCCTTTG TCGAGGTGGA AATTATTGGG TTACCTGTTG ACTGCTGTAA AGATCAGACC 
AGGGTGGTTG ATGACAATGG GTTTAATCCT GTTTGGGAGG AAACGCTCAC TTTTACCATC 
CATATGCCTG AAATAGCTTT GGTTCGATTT CTTGTTTGGG ATCATGACCC CATTGGGCGA 
GACTTCGTTG GACAAAGAAC GCTGGCCTTT AGCAGTCTTG TACCTGGTTA TCGACATGTG 
TATTTAGAAG GGCTGACAGA AGCTTCCATA TTTGTTCACA TAACCATTAA TGAAATCTAT 
GGGAAGTGGA GCCCCTTAAT CCTCAATCCC AGTTACACAA TATTGCACTT TCTAGGAGCC 
ACAAAGAGTA AGCAACTGAT CGGACTCCGA GGGCTGTTTA ACAAGAACCC AAAGCACAGT 
TCTGCTGAAA GCAGCGGTCA TTATGTACGG AAGCGTTCTA TTGGTGACCG AATTCTCCGG 
CGCACGGCCA GTGCTCCAGC AAAAGGTAGA AAGAAAAGCA AGATGGGTTT TCTGGAAGCT 
TCTGAAATTA AAGACAGTGC ATCCGAACCA GCAGACCTGA AGGACAAAGA AGGAGTTGTA 
AGGCGTACAA GCCGGAGTTT GCAAGCACGT CCTGCTTCTA TGCCAGTGGA CAAATTCCTT 
CTTGTAGGAC TGCCGTGCAC AGAAGATGAA ACTGCTCAAG ATGCCAAAGG AAAAGACAGT 
GCAACTGCCA ACAGTGATGG CGATAAAACT GAGAAGGAAA CAAACTTGAA GGAATCTCTT 
TTCACGTGTT CTGAGAAAAC TGCAGATCAT TCAAACTTGG CATCTCCCTT GAAAAAGGAG 
AGCGCCCAGA AGGAAACTAC AGCTGATTTT TCTCTTGTAC CCCAGGAGCA GCCCGAGCAT 
TCGGTTGCTG TAAGTGATGT AACCGAAACA AACCGCCCCA TAAGCAATCA GGAAAGTCTC 
CTTCCTGCTG AAACGGTGCC TCTGATTCCG GACTCTGCCT TTGAGCGCAT CACAGAAAAA 
ATACAGGGCA AGAGCTGTGC AGGCAGTAGA AGGGCAGATG ACACAAACAG GTGTGAGGAG 
AAAATAACTC CTGTCAGGAC TTGCCTTCCT GAAAAAGTAG AGGAGGTTGA AAATCTCAAA 
TACGACATCA TCCACAGAAG CACTGTGGCG TGTCTTTCTG TGACAGATAG TTCTCCTGCC 
GTCTACTCTG ATGCTCCTGG TTTATGCTCC TCTTCAACCA TGCAAGACAG CGACATCTCC 
CGCCTCATAG ACGAAGTTTC TTTGGTGACT GAGAGCGAGA TGGACAGCGC TGTCTCAGCT 
CTGATCGGCC AGCTGGATGT CACCGATGAC CAAACCAGCC TCACCGCACC GATGCTCCCT 
GGCGCTGGCA GCCGGGCCTT CAGTGCTGTG GCCACACGCA CACTGACTGC AGAGCTCAGC 
ACTCCCTGTA AGAAGGACTT CATTGCCACA CAGTCCAGCA AGTCCCCGTT ATTTTCAACT 
CCAGACTCTG CCGTGTTCCC CAGCCCCGAG ACTGCGGAGT ATTCTGTGTA CACAATTATC 
CACGAGGCAA CTCTTACACC CGGTTCTGAA TTTAAGACCC ACAGTGGGTT AGTTTGCAAG 
AAGTCAAAGA GTGCAGAAAA TAACTTGCCT AGCTCTTTTT GTTCTTCACC TTTCTCTTTG 
CTGAATGCAA ATCCCCAAAG AAAATGTGAA ACAAGCTGGG AAGCCCCCGG CTCTGCGGGT 
TCTTTTGCTT CCAGTAGCCT CATCTTTGAG GAGATGCTTG TGGACCCTCC CATGGGCGAG 
AATTCGGAAA GCAGCAGTCT TGTAGAGTTA GGTGGGGATT CCCAAGACCT CTTGGTAACT 
GCGTGCGCGT ACAAAAGGGA AGATGTGAGC CAATTGGCTT CCCCTTTAAA ACTGAGGCAG 
CAGCAGGACA GCAGGTACTG TGGTGAGAGG ACCTTTGGAA ACTGTGCAAG TGCTGGGCTC 
TGTGCTGCTG CCCTGGACTG CTCGGATAAC TTTAGGACTG CGGGAAGCAA GCTCCCTTTT 
CCAGCCTGTG AAAATGTTGG TTTTCTACAT CATCGTCACA CAGATGAGCA GAAAGATTTG 
ATGTGTACCT CCAGGAGGTC CCAACTCGAT GTGCACTCAC CAGTGCTCAG AAGCGCGTTG 
GCTTTCCCAC CCTCCCCAGC CATCAAACAG ACCAGCCCTT GCAAGTCCAA GAGCCTTGGT 
GACTTGACAT CGGAAGATAT TTCCTGCAAT TTTGAAAGTA AGTATCAGTA CATAAGTAGG 
AGCTTTGTCA CATCTGGCAT GAGAGACAAG AAGCTCGCTG CCATGAAGAC GATGAGACCG 
CACTCTACGG ATGCTTTGAC GGAACAGCTG AGAAAGCTGC TGTCCCTGGA CCAGGACGAC 
AGCCAGCAGA TGCTGTATTC CAGGCGGCTC GACGAGGACT GCCCAAAGGC ACTGGTCAGA 
AAGCTGTCGT CCAGGAGCCA GAGCAGAGTG CGGAACATCG CCAGCCGTGC CAAGGAGAGG 
CAGGAGGCCA GCAAGCAAAA ATCTTCCACC GCAGGCAGCA TAGCGGGGGT CGTCCTTCGG 
AACAAGCCTT CGGCGTCCCC TCACGTCGTC AACAGGCACT CGACGGGCTC CTACATAGCC 
AGGTACCTGG GCAGCTTCCC TGAGGAGGCA GCAGAGGGCC GGGGCCGACC TGAGGGCACG 
TGTGCTGCCT GGCACCGCGG CTGTGCTGAG CGGCTGTGTG CGCACAGCCC CCTGCTGCAG 
CTGGAGCCCG ACGGCGACGA GAAGCCTGAA GTCTATTTCC TGCTGAGGCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147307.1
CDS	765..5858
Translation	MADLEVYKNLSPEKVERCMSVMQSGTQMVKLKSGTKGLVRLFYLDEHRTCIRWRPSRKSEKAKIVIDSIYKVTEGRQSEIFHRHAEGSFDPSCCFTIYHGNHLESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDHWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLNFEEFSVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTPEYCLDIIQKFEVSEENKEQNVLGIEGFTNFMRSPACDVFNPLHCEVHQDMDQPLCNYFIASSHNTYLTGDQLLSQSRVEMYARVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKIFFRDVAETINKYAFIKNEFPVILSIENHCSIQQQKKIAQYLKEILGDKLDLSSVVTGDSRQLPSPQNLKGKILVKGKKLPYSLGADAEEGEVSDEDSADEIEDDCKLKSCYSNGATEHQVESFIRTKLESLLKESQIRDKEDPDSFTVRALLRATHEGLSVNLKQNLDTKEGGKKSHSRSLMGNFGKHKKTGKATSKCSTSDDDENQQNSSGKEAGQLHSRLTRRRKTVKLCRALSDLVVYTNSVAAQDIVDDGSTGNVLSFSETRAHQAVQQKAEQFMLYNQKQLTRVYPSAYRIDSSNFNPLPYWNVGCQLVALNYQSEGRMMQLNEAKFRVNGNCGYVLKPQQMCKGSFNPYSSDPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTIHMPEIALVRFLVWDHDPIGRDFVGQRTLAFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSKQLIGLRGLFNKNPKHSSAESSGHYVRKRSIGDRILRRTASAPAKGRKKSKMGFLEASEIKDSASEPADLKDKEGVVRRTSRSLQARPASMPVDKFLLVGLPCTEDETAQDAKGKDSATANSDGDKTEKETNLKESLFTCSEKTADHSNLASPLKKESAQKETTADFSLVPQEQPEHSVAVSDVTETNRPISNQESLLPAETVPLIPDSAFERITEKIQGKSCAGSRRADDTNRCEEKITPVRTCLPEKVEEVENLKYDIIHRSTVACLSVTDSSPAVYSDAPGLCSSSTMQDSDISRLIDEVSLVTESEMDSAVSALIGQLDVTDDQTSLTAPMLPGAGSRAFSAVATRTLTAELSTPCKKDFIATQSSKSPLFSTPDSAVFPSPETAEYSVYTIIHEATLTPGSEFKTHSGLVCKKSKSAENNLPSSFCSSPFSLLNANPQRKCETSWEAPGSAGSFASSSLIFEEMLVDPPMGENSESSSLVELGGDSQDLLVTACAYKREDVSQLASPLKLRQQQDSRYCGERTFGNCASAGLCAAALDCSDNFRTAGSKLPFPACENVGFLHHRHTDEQKDLMCTSRRSQLDVHSPVLRSALAFPPSPAIKQTSPCKSKSLGDLTSEDISCNFESKYQYISRSFVTSGMRDKKLAAMKTMRPHSTDALTEQLRKLLSLDQDDSQQMLYSRRLDEDCPKALVRKLSSRSQSRVRNIASRAKERQEASKQKSSTAGSIAGVVLRNKPSASPHVVNRHSTGSYIARYLGSFPEEAAEGRGRPEGTCAAWHRGCAERLCAHSPLLQLEPDGDEKPEVYFLLRL

Target ORF information:

RefSeq Version	XM_015291821.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291821.1

ORF Insert Sequence:

ATGGCAGACC TTGAAGTATA TAAAAATTTA AGTCCTGAAA AAGTTGAAAG ATGCATGAGT 
GTTATGCAGT CTGGGACTCA GATGGTTAAG CTGAAGAGTG GAACCAAAGG GCTCGTTCGT 
CTCTTCTACC TGGACGAGCA CAGGACCTGC ATCCGATGGA GACCGTCCAG AAAAAGTGAA 
AAGGCAAAAA TTGTCATTGA TTCTATTTAC AAAGTGACCG AAGGCCGTCA GTCTGAAATC 
TTCCACCGCC ATGCAGAGGG GAGCTTTGAC CCGAGCTGTT GCTTTACCAT TTACCATGGC 
AACCACCTGG AGTCACTGGA TCTGATTACC TCTAACCCAG AGGAAGCTCG CACCTGGATC 
ACAGGGCTGA AATATTTGAT GGCTGGGATA AGCGATGAGG ACTCGCTTGC TAAGCGACAG 
AGAACTCATG ATCACTGGGT GAAACAAACT TTTGAGGAAG CTGACAAGAA TGGAGATGGC 
CTGCTGAACA TTGAAGAGAT CCACCAGCTG ATGCATAAGC TAAATGTTAA CCTGCCCCGC 
AGGAAGGTCC GGCAGATGTT TCAGGAAGCT GACACAGATG AGAACCAAGG AACTCTAAAT 
TTTGAAGAAT TTTCAGTGTT TTACAAGATG ATGTCCTTGC GTCGAGACCT GTACTTACTG 
CTCTTAAGCT ACAGTGACAA GAAAGATCAC CTCACCGTTG AAGAACTTGC TCAATTTCTG 
AAGGTGGAAC AAAAGATGAG TAACGTGACG CCAGAATATT GTCTTGACAT CATACAGAAG 
TTTGAAGTAT CAGAAGAAAA CAAGGAGCAG AACGTGCTTG GGATTGAAGG TTTCACCAAC 
TTCATGCGCA GTCCTGCTTG TGATGTTTTT AATCCATTGC ATTGCGAAGT GCATCAGGAT 
ATGGATCAAC CCCTTTGTAA CTACTTCATT GCTTCATCTC ACAACACGTA CCTGACAGGA 
GACCAGCTGC TGTCCCAGTC TAGGGTGGAG ATGTATGCAC GAGTGCTGCA GGACGGTTGT 
CGATGTATTG AAGTGGATTG CTGGGATGGT CCAGATGGAG AGCCAGTAGT GCATCATGGG 
TATACGCTTA CCTCTAAAAT ATTCTTCCGT GACGTGGCAG AAACTATCAA TAAGTATGCC 
TTCATCAAAA ATGAGTTTCC GGTGATACTG TCTATTGAAA ATCATTGCAG TATTCAGCAG 
CAAAAGAAGA TTGCTCAGTA CTTGAAGGAG ATACTTGGTG ACAAATTGGA CTTGTCATCT 
GTGGTCACCG GAGATTCCAG GCAGCTTCCT AGCCCTCAAA ACTTGAAAGG GAAAATCCTA 
GTGAAGGGAA AAAAGTTGCC ATACAGTCTT GGAGCAGATG CTGAGGAAGG AGAAGTTTCT 
GATGAGGACA GTGCTGATGA AATTGAAGAC GACTGCAAGC TGAAATCCTG CTATAGTAAC 
GGTGCAACTG AGCATCAGGT GGAATCTTTT ATAAGAACAA AGCTTGAATC CCTGCTAAAG 
GAGTCCCAGA TCAGGGACAA AGAAGATCCT GACAGCTTCA CGGTGAGAGC ACTGCTAAGG 
GCAACTCACG AGGGTTTAAG TGTTAACCTG AAGCAGAACC TGGACACAAA AGAAGGTGGG 
AAGAAGTCTC ATAGTCGATC ATTAATGGGC AACTTTGGTA AACACAAAAA AACAGGGAAG 
GCAACATCCA AATGTAGCAC ATCAGATGAT GATGAAAATC AGCAAAACTC TTCTGGAAAG 
GAAGCAGGAC AGCTGCACAG TAGACTGACT CGTCGAAGGA AAACTGTGAA GCTGTGCCGA 
GCTCTGTCTG ACCTAGTGGT GTACACGAAC TCTGTGGCAG CCCAGGATAT AGTAGATGAT 
GGATCAACAG GGAATGTGCT GTCATTCAGT GAAACAAGAG CCCACCAAGC AGTGCAGCAG 
AAGGCTGAAC AGTTCATGCT TTACAACCAG AAACAGCTTA CAAGGGTTTA TCCATCAGCG 
TATCGGATTG ACTCCAGCAA TTTCAATCCT TTACCTTACT GGAATGTTGG TTGCCAGCTA 
GTGGCACTAA ACTACCAGTC TGAGGGACGT ATGATGCAAT TAAACGAAGC AAAATTTAGG 
GTAAATGGCA ACTGTGGTTA CGTCCTCAAA CCTCAGCAGA TGTGCAAAGG CTCATTCAAC 
CCCTATTCTT CAGATCCACT TCCTGCTAGT CCTAAGAAGC AGCTCATCCT GAAAATCATC 
AGTGGACAGC AGCTGCCTAA GCCTCCTGAC TCAATGCTAG GAGACAGAGG AGAGATAATA 
GATCCCTTTG TCGAGGTGGA AATTATTGGG TTACCTGTTG ACTGCTGTAA AGATCAGACC 
AGGGTGGTTG ATGACAATGG GTTTAATCCT GTTTGGGAGG AAACGCTCAC TTTTACCATC 
CATATGCCTG AAATAGCTTT GGTTCGATTT CTTGTTTGGG ATCATGACCC CATTGGGCGA 
GACTTCGTTG GACAAAGAAC GCTGGCCTTT AGCAGTCTTG TACCTGGTTA TCGACATGTG 
TATTTAGAAG GGCTGACAGA AGCTTCCATA TTTGTTCACA TAACCATTAA TGAAATCTAT 
GGGAAGTGGA GCCCCTTAAT CCTCAATCCC AGTTACACAA TATTGCACTT TCTAGGAGCC 
ACAAAGAGTA AGCAACTGAT CGGACTCCGA GGGCTGTTTA ACAAGAACCC AAAGCACAGT 
TCTGCTGAAA GCAGCGGTCA TTATGTACGG AAGCGTTCTA TTGGTGACCG AATTCTCCGG 
CGCACGGCCA GTGCTCCAGC AAAAGGTAGA AAGAAAAGCA AGATGGGTTT TCTGGAAGCT 
TCTGAAATTA AAGACAGTGC ATCCGAACCA GCAGACCTGA AGGACAAAGA AGGAGTTGTA 
AGGCGTACAA GCCGGAGTTT GCAAGCACGT CCTGCTTCTA TGCCAGTGGA CAAATTCCTT 
CTTGTAGGAC TGCCGTGCAC AGAAGATGAA ACTGCTCAAG ATGCCAAAGG AAAAGACAGT 
GCAACTGCCA ACAGTGATGG CGATAAAACT GAGAAGGAAA CAAACTTGAA GGAATCTCTT 
TTCACGTGTT CTGAGAAAAC TGCAGATCAT TCAAACTTGG CATCTCCCTT GAAAAAGGAG 
AGCGCCCAGA AGGAAACTAC AGCTGATTTT TCTCTTGTAC CCCAGGAGCA GCCCGAGCAT 
TCGGTTGCTG TAAGTGATGT AACCGAAACA AACCGCCCCA TAAGCAATCA GGAAAGTCTC 
CTTCCTGCTG AAACGGTGCC TCTGATTCCG GACTCTGCCT TTGAGCGCAT CACAGAAAAA 
ATACAGGGCA AGAGCTGTGC AGGCAGTAGA AGGGCAGATG ACACAAACAG GTGTGAGGAG 
AAAATAACTC CTGTCAGGAC TTGCCTTCCT GAAAAAGTAG AGGAGGTTGA AAATCTCAAA 
TACGACATCA TCCACAGAAG CACTGTGGCG TGTCTTTCTG TGACAGATAG TTCTCCTGCC 
GTCTACTCTG ATGCTCCTGG TTTATGCTCC TCTTCAACCA TGCAAGACAG CGACATCTCC 
CGCCTCATAG ACGAAGTTTC TTTGGTGACT GAGAGCGAGA TGGACAGCGC TGTCTCAGCT 
CTGATCGGCC AGCTGGATGT CACCGATGAC CAAACCAGCC TCACCGCACC GATGCTCCCT 
GGCGCTGGCA GCCGGGCCTT CAGTGCTGTG GCCACACGCA CACTGACTGC AGAGCTCAGC 
ACTCCCTGTA AGAAGGACTT CATTGCCACA CAGTCCAGCA AGTCCCCGTT ATTTTCAACT 
CCAGACTCTG CCGTGTTCCC CAGCCCCGAG ACTGCGGAGT ATTCTGTGTA CACAATTATC 
CACGAGGCAA CTCTTACACC CGGTTCTGAA TTTAAGACCC ACAGTGGGTT AGTTTGCAAG 
AAGTCAAAGA GTGCAGAAAA TAACTTGCCT AGCTCTTTTT GTTCTTCACC TTTCTCTTTG 
CTGAATGCAA ATCCCCAAAG AAAATGTGAA ACAAGCTGGG AAGCCCCCGG CTCTGCGGGT 
TCTTTTGCTT CCAGTAGCCT CATCTTTGAG GAGATGCTTG TGGACCCTCC CATGGGCGAG 
AATTCGGAAA GCAGCAGTCT TGTAGAGTTA GGTGGGGATT CCCAAGACCT CTTGGTAACT 
GCGTGCGCGT ACAAAAGGGA AGATGTGAGC CAATTGGCTT CCCCTTTAAA ACTGAGGCAG 
CAGCAGGACA GCAGGTACTG TGGTGAGAGG ACCTTTGGAA ACTGTGCAAG TGCTGGGCTC 
TGTGCTGCTG CCCTGGACTG CTCGGATAAC TTTAGGACTG CGGGAAGCAA GCTCCCTTTT 
CCAGCCTGTG AAAATGTTGG TTTTCTACAT CATCGTCACA CAGATGAGCA GAAAGATTTG 
ATGTGTACCT CCAGGAGGTC CCAACTCGAT GTGCACTCAC CAGTGCTCAG AAGCGCGTTG 
GCTTTCCCAC CCTCCCCAGC CATCAAACAG ACCAGCCCTT GCAAGTCCAA GAGCCTTGGT 
GACTTGACAT CGGAAGATAT TTCCTGCAAT TTTGAAAGTA AGTATCAGTA CATAAGTAGG 
AGCTTTGTCA CATCTGGCAT GAGAGACAAG AAGCTCGCTG CCATGAAGAC GATGAGACCG 
CACTCTACGG ATGCTTTGAC GGAACAGCTG AGAAAGCTGC TGTCCCTGGA CCAGGACGAC 
AGCCAGCAGA TGCTGTATTC CAGGCGGCTC GACGAGGACT GCCCAAAGGC ACTGGTCAGA 
AAGCTGTCGT CCAGGAGCCA GAGCAGAGTG CGGAACATCG CCAGCCGTGC CAAGGAGAGG 
CAGGAGGCCA GCAAGCAAAA ATCTTCCACC GCAGGCAGCA TAGCGGGGGT CGTCCTTCGG 
AACAAGCCTT CGGCGTCCCC TCACGTCGTC AACAGGCACT CGACGGGCTC CTACATAGCC 
AGGTACCTGG GCAGCTTCCC TGAGGAGGCA GCAGAGGGCC GGGGCCGACC TGAGGGCACG 
TGTGCTGCCT GGCACCGCGG CTGTGCTGAG CGGCTGTGTG CGCACAGCCC CCTGCTGCAG 
CTGGAGCCCG ACGGCGACGA GAAGCCTGAA GTCTATTTCC TGCTGAGGCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_015291821.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147307.1 Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X8, mRNA.

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CloneID	OGa40161
Clone ID Related Accession (Same CDS sequence)	XM_015291821.1 , XM_015291821.2
Accession Version	XM_015291821.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291821.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACC TTGAAGTATA TAAAAATTTA AGTCCTGAAA AAGTTGAAAG ATGCATGAGT 
GTTATGCAGT CTGGGACTCA GATGGTTAAG CTGAAGAGTG GAACCAAAGG GCTCGTTCGT 
CTCTTCTACC TGGACGAGCA CAGGACCTGC ATCCGATGGA GACCGTCCAG AAAAAGTGAA 
AAGGCAAAAA TTGTCATTGA TTCTATTTAC AAAGTGACCG AAGGCCGTCA GTCTGAAATC 
TTCCACCGCC ATGCAGAGGG GAGCTTTGAC CCGAGCTGTT GCTTTACCAT TTACCATGGC 
AACCACCTGG AGTCACTGGA TCTGATTACC TCTAACCCAG AGGAAGCTCG CACCTGGATC 
ACAGGGCTGA AATATTTGAT GGCTGGGATA AGCGATGAGG ACTCGCTTGC TAAGCGACAG 
AGAACTCATG ATCACTGGGT GAAACAAACT TTTGAGGAAG CTGACAAGAA TGGAGATGGC 
CTGCTGAACA TTGAAGAGAT CCACCAGCTG ATGCATAAGC TAAATGTTAA CCTGCCCCGC 
AGGAAGGTCC GGCAGATGTT TCAGGAAGCT GACACAGATG AGAACCAAGG AACTCTAAAT 
TTTGAAGAAT TTTCAGTGTT TTACAAGATG ATGTCCTTGC GTCGAGACCT GTACTTACTG 
CTCTTAAGCT ACAGTGACAA GAAAGATCAC CTCACCGTTG AAGAACTTGC TCAATTTCTG 
AAGGTGGAAC AAAAGATGAG TAACGTGACG CCAGAATATT GTCTTGACAT CATACAGAAG 
TTTGAAGTAT CAGAAGAAAA CAAGGAGCAG AACGTGCTTG GGATTGAAGG TTTCACCAAC 
TTCATGCGCA GTCCTGCTTG TGATGTTTTT AATCCATTGC ATTGCGAAGT GCATCAGGAT 
ATGGATCAAC CCCTTTGTAA CTACTTCATT GCTTCATCTC ACAACACGTA CCTGACAGGA 
GACCAGCTGC TGTCCCAGTC TAGGGTGGAG ATGTATGCAC GAGTGCTGCA GGACGGTTGT 
CGATGTATTG AAGTGGATTG CTGGGATGGT CCAGATGGAG AGCCAGTAGT GCATCATGGG 
TATACGCTTA CCTCTAAAAT ATTCTTCCGT GACGTGGCAG AAACTATCAA TAAGTATGCC 
TTCATCAAAA ATGAGTTTCC GGTGATACTG TCTATTGAAA ATCATTGCAG TATTCAGCAG 
CAAAAGAAGA TTGCTCAGTA CTTGAAGGAG ATACTTGGTG ACAAATTGGA CTTGTCATCT 
GTGGTCACCG GAGATTCCAG GCAGCTTCCT AGCCCTCAAA ACTTGAAAGG GAAAATCCTA 
GTGAAGGGAA AAAAGTTGCC ATACAGTCTT GGAGCAGATG CTGAGGAAGG AGAAGTTTCT 
GATGAGGACA GTGCTGATGA AATTGAAGAC GACTGCAAGC TGAAATCCTG CTATAGTAAC 
GGTGCAACTG AGCATCAGGT GGAATCTTTT ATAAGAACAA AGCTTGAATC CCTGCTAAAG 
GAGTCCCAGA TCAGGGACAA AGAAGATCCT GACAGCTTCA CGGTGAGAGC ACTGCTAAGG 
GCAACTCACG AGGGTTTAAG TGTTAACCTG AAGCAGAACC TGGACACAAA AGAAGGTGGG 
AAGAAGTCTC ATAGTCGATC ATTAATGGGC AACTTTGGTA AACACAAAAA AACAGGGAAG 
GCAACATCCA AATGTAGCAC ATCAGATGAT GATGAAAATC AGCAAAACTC TTCTGGAAAG 
GAAGCAGGAC AGCTGCACAG TAGACTGACT CGTCGAAGGA AAACTGTGAA GCTGTGCCGA 
GCTCTGTCTG ACCTAGTGGT GTACACGAAC TCTGTGGCAG CCCAGGATAT AGTAGATGAT 
GGATCAACAG GGAATGTGCT GTCATTCAGT GAAACAAGAG CCCACCAAGC AGTGCAGCAG 
AAGGCTGAAC AGTTCATGCT TTACAACCAG AAACAGCTTA CAAGGGTTTA TCCATCAGCG 
TATCGGATTG ACTCCAGCAA TTTCAATCCT TTACCTTACT GGAATGTTGG TTGCCAGCTA 
GTGGCACTAA ACTACCAGTC TGAGGGACGT ATGATGCAAT TAAACGAAGC AAAATTTAGG 
GTAAATGGCA ACTGTGGTTA CGTCCTCAAA CCTCAGCAGA TGTGCAAAGG CTCATTCAAC 
CCCTATTCTT CAGATCCACT TCCTGCTAGT CCTAAGAAGC AGCTCATCCT GAAAATCATC 
AGTGGACAGC AGCTGCCTAA GCCTCCTGAC TCAATGCTAG GAGACAGAGG AGAGATAATA 
GATCCCTTTG TCGAGGTGGA AATTATTGGG TTACCTGTTG ACTGCTGTAA AGATCAGACC 
AGGGTGGTTG ATGACAATGG GTTTAATCCT GTTTGGGAGG AAACGCTCAC TTTTACCATC 
CATATGCCTG AAATAGCTTT GGTTCGATTT CTTGTTTGGG ATCATGACCC CATTGGGCGA 
GACTTCGTTG GACAAAGAAC GCTGGCCTTT AGCAGTCTTG TACCTGGTTA TCGACATGTG 
TATTTAGAAG GGCTGACAGA AGCTTCCATA TTTGTTCACA TAACCATTAA TGAAATCTAT 
GGGAAGTGGA GCCCCTTAAT CCTCAATCCC AGTTACACAA TATTGCACTT TCTAGGAGCC 
ACAAAGAGTA AGCAACTGAT CGGACTCCGA GGGCTGTTTA ACAAGAACCC AAAGCACAGT 
TCTGCTGAAA GCAGCGGTCA TTATGTACGG AAGCGTTCTA TTGGTGACCG AATTCTCCGG 
CGCACGGCCA GTGCTCCAGC AAAAGGTAGA AAGAAAAGCA AGATGGGTTT TCTGGAAGCT 
TCTGAAATTA AAGACAGTGC ATCCGAACCA GCAGACCTGA AGGACAAAGA AGGAGTTGTA 
AGGCGTACAA GCCGGAGTTT GCAAGCACGT CCTGCTTCTA TGCCAGTGGA CAAATTCCTT 
CTTGTAGGAC TGCCGTGCAC AGAAGATGAA ACTGCTCAAG ATGCCAAAGG AAAAGACAGT 
GCAACTGCCA ACAGTGATGG CGATAAAACT GAGAAGGAAA CAAACTTGAA GGAATCTCTT 
TTCACGTGTT CTGAGAAAAC TGCAGATCAT TCAAACTTGG CATCTCCCTT GAAAAAGGAG 
AGCGCCCAGA AGGAAACTAC AGCTGATTTT TCTCTTGTAC CCCAGGAGCA GCCCGAGCAT 
TCGGTTGCTG TAAGTGATGT AACCGAAACA AACCGCCCCA TAAGCAATCA GGAAAGTCTC 
CTTCCTGCTG AAACGGTGCC TCTGATTCCG GACTCTGCCT TTGAGCGCAT CACAGAAAAA 
ATACAGGGCA AGAGCTGTGC AGGCAGTAGA AGGGCAGATG ACACAAACAG GTGTGAGGAG 
AAAATAACTC CTGTCAGGAC TTGCCTTCCT GAAAAAGTAG AGGAGGTTGA AAATCTCAAA 
TACGACATCA TCCACAGAAG CACTGTGGCG TGTCTTTCTG TGACAGATAG TTCTCCTGCC 
GTCTACTCTG ATGCTCCTGG TTTATGCTCC TCTTCAACCA TGCAAGACAG CGACATCTCC 
CGCCTCATAG ACGAAGTTTC TTTGGTGACT GAGAGCGAGA TGGACAGCGC TGTCTCAGCT 
CTGATCGGCC AGCTGGATGT CACCGATGAC CAAACCAGCC TCACCGCACC GATGCTCCCT 
GGCGCTGGCA GCCGGGCCTT CAGTGCTGTG GCCACACGCA CACTGACTGC AGAGCTCAGC 
ACTCCCTGTA AGAAGGACTT CATTGCCACA CAGTCCAGCA AGTCCCCGTT ATTTTCAACT 
CCAGACTCTG CCGTGTTCCC CAGCCCCGAG ACTGCGGAGT ATTCTGTGTA CACAATTATC 
CACGAGGCAA CTCTTACACC CGGTTCTGAA TTTAAGACCC ACAGTGGGTT AGTTTGCAAG 
AAGTCAAAGA GTGCAGAAAA TAACTTGCCT AGCTCTTTTT GTTCTTCACC TTTCTCTTTG 
CTGAATGCAA ATCCCCAAAG AAAATGTGAA ACAAGCTGGG AAGCCCCCGG CTCTGCGGGT 
TCTTTTGCTT CCAGTAGCCT CATCTTTGAG GAGATGCTTG TGGACCCTCC CATGGGCGAG 
AATTCGGAAA GCAGCAGTCT TGTAGAGTTA GGTGGGGATT CCCAAGACCT CTTGGTAACT 
GCGTGCGCGT ACAAAAGGGA AGATGTGAGC CAATTGGCTT CCCCTTTAAA ACTGAGGCAG 
CAGCAGGACA GCAGGTACTG TGGTGAGAGG ACCTTTGGAA ACTGTGCAAG TGCTGGGCTC 
TGTGCTGCTG CCCTGGACTG CTCGGATAAC TTTAGGACTG CGGGAAGCAA GCTCCCTTTT 
CCAGCCTGTG AAAATGTTGG TTTTCTACAT CATCGTCACA CAGATGAGCA GAAAGATTTG 
ATGTGTACCT CCAGGAGGTC CCAACTCGAT GTGCACTCAC CAGTGCTCAG AAGCGCGTTG 
GCTTTCCCAC CCTCCCCAGC CATCAAACAG ACCAGCCCTT GCAAGTCCAA GAGCCTTGGT 
GACTTGACAT CGGAAGATAT TTCCTGCAAT TTTGAAAGTA AGTATCAGTA CATAAGTAGG 
AGCTTTGTCA CATCTGGCAT GAGAGACAAG AAGCTCGCTG CCATGAAGAC GATGAGACCG 
CACTCTACGG ATGCTTTGAC GGAACAGCTG AGAAAGCTGC TGTCCCTGGA CCAGGACGAC 
AGCCAGCAGA TGCTGTATTC CAGGCGGCTC GACGAGGACT GCCCAAAGGC ACTGGTCAGA 
AAGCTGTCGT CCAGGAGCCA GAGCAGAGTG CGGAACATCG CCAGCCGTGC CAAGGAGAGG 
CAGGAGGCCA GCAAGCAAAA ATCTTCCACC GCAGGCAGCA TAGCGGGGGT CGTCCTTCGG 
AACAAGCCTT CGGCGTCCCC TCACGTCGTC AACAGGCACT CGACGGGCTC CTACATAGCC 
AGGTACCTGG GCAGCTTCCC TGAGGAGGCA GCAGAGGGCC GGGGCCGACC TGAGGGCACG 
TGTGCTGCCT GGCACCGCGG CTGTGCTGAG CGGCTGTGTG CGCACAGCCC CCTGCTGCAG 
CTGGAGCCCG ACGGCGACGA GAAGCCTGAA GTCTATTTCC TGCTGAGGCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147307.1
CDS	629..5722
Translation	MADLEVYKNLSPEKVERCMSVMQSGTQMVKLKSGTKGLVRLFYLDEHRTCIRWRPSRKSEKAKIVIDSIYKVTEGRQSEIFHRHAEGSFDPSCCFTIYHGNHLESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDHWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLNFEEFSVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTPEYCLDIIQKFEVSEENKEQNVLGIEGFTNFMRSPACDVFNPLHCEVHQDMDQPLCNYFIASSHNTYLTGDQLLSQSRVEMYARVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKIFFRDVAETINKYAFIKNEFPVILSIENHCSIQQQKKIAQYLKEILGDKLDLSSVVTGDSRQLPSPQNLKGKILVKGKKLPYSLGADAEEGEVSDEDSADEIEDDCKLKSCYSNGATEHQVESFIRTKLESLLKESQIRDKEDPDSFTVRALLRATHEGLSVNLKQNLDTKEGGKKSHSRSLMGNFGKHKKTGKATSKCSTSDDDENQQNSSGKEAGQLHSRLTRRRKTVKLCRALSDLVVYTNSVAAQDIVDDGSTGNVLSFSETRAHQAVQQKAEQFMLYNQKQLTRVYPSAYRIDSSNFNPLPYWNVGCQLVALNYQSEGRMMQLNEAKFRVNGNCGYVLKPQQMCKGSFNPYSSDPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTIHMPEIALVRFLVWDHDPIGRDFVGQRTLAFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSKQLIGLRGLFNKNPKHSSAESSGHYVRKRSIGDRILRRTASAPAKGRKKSKMGFLEASEIKDSASEPADLKDKEGVVRRTSRSLQARPASMPVDKFLLVGLPCTEDETAQDAKGKDSATANSDGDKTEKETNLKESLFTCSEKTADHSNLASPLKKESAQKETTADFSLVPQEQPEHSVAVSDVTETNRPISNQESLLPAETVPLIPDSAFERITEKIQGKSCAGSRRADDTNRCEEKITPVRTCLPEKVEEVENLKYDIIHRSTVACLSVTDSSPAVYSDAPGLCSSSTMQDSDISRLIDEVSLVTESEMDSAVSALIGQLDVTDDQTSLTAPMLPGAGSRAFSAVATRTLTAELSTPCKKDFIATQSSKSPLFSTPDSAVFPSPETAEYSVYTIIHEATLTPGSEFKTHSGLVCKKSKSAENNLPSSFCSSPFSLLNANPQRKCETSWEAPGSAGSFASSSLIFEEMLVDPPMGENSESSSLVELGGDSQDLLVTACAYKREDVSQLASPLKLRQQQDSRYCGERTFGNCASAGLCAAALDCSDNFRTAGSKLPFPACENVGFLHHRHTDEQKDLMCTSRRSQLDVHSPVLRSALAFPPSPAIKQTSPCKSKSLGDLTSEDISCNFESKYQYISRSFVTSGMRDKKLAAMKTMRPHSTDALTEQLRKLLSLDQDDSQQMLYSRRLDEDCPKALVRKLSSRSQSRVRNIASRAKERQEASKQKSSTAGSIAGVVLRNKPSASPHVVNRHSTGSYIARYLGSFPEEAAEGRGRPEGTCAAWHRGCAERLCAHSPLLQLEPDGDEKPEVYFLLRL

Target ORF information:

RefSeq Version	XM_015291821.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291821.2

ORF Insert Sequence:

ATGGCAGACC TTGAAGTATA TAAAAATTTA AGTCCTGAAA AAGTTGAAAG ATGCATGAGT 
GTTATGCAGT CTGGGACTCA GATGGTTAAG CTGAAGAGTG GAACCAAAGG GCTCGTTCGT 
CTCTTCTACC TGGACGAGCA CAGGACCTGC ATCCGATGGA GACCGTCCAG AAAAAGTGAA 
AAGGCAAAAA TTGTCATTGA TTCTATTTAC AAAGTGACCG AAGGCCGTCA GTCTGAAATC 
TTCCACCGCC ATGCAGAGGG GAGCTTTGAC CCGAGCTGTT GCTTTACCAT TTACCATGGC 
AACCACCTGG AGTCACTGGA TCTGATTACC TCTAACCCAG AGGAAGCTCG CACCTGGATC 
ACAGGGCTGA AATATTTGAT GGCTGGGATA AGCGATGAGG ACTCGCTTGC TAAGCGACAG 
AGAACTCATG ATCACTGGGT GAAACAAACT TTTGAGGAAG CTGACAAGAA TGGAGATGGC 
CTGCTGAACA TTGAAGAGAT CCACCAGCTG ATGCATAAGC TAAATGTTAA CCTGCCCCGC 
AGGAAGGTCC GGCAGATGTT TCAGGAAGCT GACACAGATG AGAACCAAGG AACTCTAAAT 
TTTGAAGAAT TTTCAGTGTT TTACAAGATG ATGTCCTTGC GTCGAGACCT GTACTTACTG 
CTCTTAAGCT ACAGTGACAA GAAAGATCAC CTCACCGTTG AAGAACTTGC TCAATTTCTG 
AAGGTGGAAC AAAAGATGAG TAACGTGACG CCAGAATATT GTCTTGACAT CATACAGAAG 
TTTGAAGTAT CAGAAGAAAA CAAGGAGCAG AACGTGCTTG GGATTGAAGG TTTCACCAAC 
TTCATGCGCA GTCCTGCTTG TGATGTTTTT AATCCATTGC ATTGCGAAGT GCATCAGGAT 
ATGGATCAAC CCCTTTGTAA CTACTTCATT GCTTCATCTC ACAACACGTA CCTGACAGGA 
GACCAGCTGC TGTCCCAGTC TAGGGTGGAG ATGTATGCAC GAGTGCTGCA GGACGGTTGT 
CGATGTATTG AAGTGGATTG CTGGGATGGT CCAGATGGAG AGCCAGTAGT GCATCATGGG 
TATACGCTTA CCTCTAAAAT ATTCTTCCGT GACGTGGCAG AAACTATCAA TAAGTATGCC 
TTCATCAAAA ATGAGTTTCC GGTGATACTG TCTATTGAAA ATCATTGCAG TATTCAGCAG 
CAAAAGAAGA TTGCTCAGTA CTTGAAGGAG ATACTTGGTG ACAAATTGGA CTTGTCATCT 
GTGGTCACCG GAGATTCCAG GCAGCTTCCT AGCCCTCAAA ACTTGAAAGG GAAAATCCTA 
GTGAAGGGAA AAAAGTTGCC ATACAGTCTT GGAGCAGATG CTGAGGAAGG AGAAGTTTCT 
GATGAGGACA GTGCTGATGA AATTGAAGAC GACTGCAAGC TGAAATCCTG CTATAGTAAC 
GGTGCAACTG AGCATCAGGT GGAATCTTTT ATAAGAACAA AGCTTGAATC CCTGCTAAAG 
GAGTCCCAGA TCAGGGACAA AGAAGATCCT GACAGCTTCA CGGTGAGAGC ACTGCTAAGG 
GCAACTCACG AGGGTTTAAG TGTTAACCTG AAGCAGAACC TGGACACAAA AGAAGGTGGG 
AAGAAGTCTC ATAGTCGATC ATTAATGGGC AACTTTGGTA AACACAAAAA AACAGGGAAG 
GCAACATCCA AATGTAGCAC ATCAGATGAT GATGAAAATC AGCAAAACTC TTCTGGAAAG 
GAAGCAGGAC AGCTGCACAG TAGACTGACT CGTCGAAGGA AAACTGTGAA GCTGTGCCGA 
GCTCTGTCTG ACCTAGTGGT GTACACGAAC TCTGTGGCAG CCCAGGATAT AGTAGATGAT 
GGATCAACAG GGAATGTGCT GTCATTCAGT GAAACAAGAG CCCACCAAGC AGTGCAGCAG 
AAGGCTGAAC AGTTCATGCT TTACAACCAG AAACAGCTTA CAAGGGTTTA TCCATCAGCG 
TATCGGATTG ACTCCAGCAA TTTCAATCCT TTACCTTACT GGAATGTTGG TTGCCAGCTA 
GTGGCACTAA ACTACCAGTC TGAGGGACGT ATGATGCAAT TAAACGAAGC AAAATTTAGG 
GTAAATGGCA ACTGTGGTTA CGTCCTCAAA CCTCAGCAGA TGTGCAAAGG CTCATTCAAC 
CCCTATTCTT CAGATCCACT TCCTGCTAGT CCTAAGAAGC AGCTCATCCT GAAAATCATC 
AGTGGACAGC AGCTGCCTAA GCCTCCTGAC TCAATGCTAG GAGACAGAGG AGAGATAATA 
GATCCCTTTG TCGAGGTGGA AATTATTGGG TTACCTGTTG ACTGCTGTAA AGATCAGACC 
AGGGTGGTTG ATGACAATGG GTTTAATCCT GTTTGGGAGG AAACGCTCAC TTTTACCATC 
CATATGCCTG AAATAGCTTT GGTTCGATTT CTTGTTTGGG ATCATGACCC CATTGGGCGA 
GACTTCGTTG GACAAAGAAC GCTGGCCTTT AGCAGTCTTG TACCTGGTTA TCGACATGTG 
TATTTAGAAG GGCTGACAGA AGCTTCCATA TTTGTTCACA TAACCATTAA TGAAATCTAT 
GGGAAGTGGA GCCCCTTAAT CCTCAATCCC AGTTACACAA TATTGCACTT TCTAGGAGCC 
ACAAAGAGTA AGCAACTGAT CGGACTCCGA GGGCTGTTTA ACAAGAACCC AAAGCACAGT 
TCTGCTGAAA GCAGCGGTCA TTATGTACGG AAGCGTTCTA TTGGTGACCG AATTCTCCGG 
CGCACGGCCA GTGCTCCAGC AAAAGGTAGA AAGAAAAGCA AGATGGGTTT TCTGGAAGCT 
TCTGAAATTA AAGACAGTGC ATCCGAACCA GCAGACCTGA AGGACAAAGA AGGAGTTGTA 
AGGCGTACAA GCCGGAGTTT GCAAGCACGT CCTGCTTCTA TGCCAGTGGA CAAATTCCTT 
CTTGTAGGAC TGCCGTGCAC AGAAGATGAA ACTGCTCAAG ATGCCAAAGG AAAAGACAGT 
GCAACTGCCA ACAGTGATGG CGATAAAACT GAGAAGGAAA CAAACTTGAA GGAATCTCTT 
TTCACGTGTT CTGAGAAAAC TGCAGATCAT TCAAACTTGG CATCTCCCTT GAAAAAGGAG 
AGCGCCCAGA AGGAAACTAC AGCTGATTTT TCTCTTGTAC CCCAGGAGCA GCCCGAGCAT 
TCGGTTGCTG TAAGTGATGT AACCGAAACA AACCGCCCCA TAAGCAATCA GGAAAGTCTC 
CTTCCTGCTG AAACGGTGCC TCTGATTCCG GACTCTGCCT TTGAGCGCAT CACAGAAAAA 
ATACAGGGCA AGAGCTGTGC AGGCAGTAGA AGGGCAGATG ACACAAACAG GTGTGAGGAG 
AAAATAACTC CTGTCAGGAC TTGCCTTCCT GAAAAAGTAG AGGAGGTTGA AAATCTCAAA 
TACGACATCA TCCACAGAAG CACTGTGGCG TGTCTTTCTG TGACAGATAG TTCTCCTGCC 
GTCTACTCTG ATGCTCCTGG TTTATGCTCC TCTTCAACCA TGCAAGACAG CGACATCTCC 
CGCCTCATAG ACGAAGTTTC TTTGGTGACT GAGAGCGAGA TGGACAGCGC TGTCTCAGCT 
CTGATCGGCC AGCTGGATGT CACCGATGAC CAAACCAGCC TCACCGCACC GATGCTCCCT 
GGCGCTGGCA GCCGGGCCTT CAGTGCTGTG GCCACACGCA CACTGACTGC AGAGCTCAGC 
ACTCCCTGTA AGAAGGACTT CATTGCCACA CAGTCCAGCA AGTCCCCGTT ATTTTCAACT 
CCAGACTCTG CCGTGTTCCC CAGCCCCGAG ACTGCGGAGT ATTCTGTGTA CACAATTATC 
CACGAGGCAA CTCTTACACC CGGTTCTGAA TTTAAGACCC ACAGTGGGTT AGTTTGCAAG 
AAGTCAAAGA GTGCAGAAAA TAACTTGCCT AGCTCTTTTT GTTCTTCACC TTTCTCTTTG 
CTGAATGCAA ATCCCCAAAG AAAATGTGAA ACAAGCTGGG AAGCCCCCGG CTCTGCGGGT 
TCTTTTGCTT CCAGTAGCCT CATCTTTGAG GAGATGCTTG TGGACCCTCC CATGGGCGAG 
AATTCGGAAA GCAGCAGTCT TGTAGAGTTA GGTGGGGATT CCCAAGACCT CTTGGTAACT 
GCGTGCGCGT ACAAAAGGGA AGATGTGAGC CAATTGGCTT CCCCTTTAAA ACTGAGGCAG 
CAGCAGGACA GCAGGTACTG TGGTGAGAGG ACCTTTGGAA ACTGTGCAAG TGCTGGGCTC 
TGTGCTGCTG CCCTGGACTG CTCGGATAAC TTTAGGACTG CGGGAAGCAA GCTCCCTTTT 
CCAGCCTGTG AAAATGTTGG TTTTCTACAT CATCGTCACA CAGATGAGCA GAAAGATTTG 
ATGTGTACCT CCAGGAGGTC CCAACTCGAT GTGCACTCAC CAGTGCTCAG AAGCGCGTTG 
GCTTTCCCAC CCTCCCCAGC CATCAAACAG ACCAGCCCTT GCAAGTCCAA GAGCCTTGGT 
GACTTGACAT CGGAAGATAT TTCCTGCAAT TTTGAAAGTA AGTATCAGTA CATAAGTAGG 
AGCTTTGTCA CATCTGGCAT GAGAGACAAG AAGCTCGCTG CCATGAAGAC GATGAGACCG 
CACTCTACGG ATGCTTTGAC GGAACAGCTG AGAAAGCTGC TGTCCCTGGA CCAGGACGAC 
AGCCAGCAGA TGCTGTATTC CAGGCGGCTC GACGAGGACT GCCCAAAGGC ACTGGTCAGA 
AAGCTGTCGT CCAGGAGCCA GAGCAGAGTG CGGAACATCG CCAGCCGTGC CAAGGAGAGG 
CAGGAGGCCA GCAAGCAAAA ATCTTCCACC GCAGGCAGCA TAGCGGGGGT CGTCCTTCGG 
AACAAGCCTT CGGCGTCCCC TCACGTCGTC AACAGGCACT CGACGGGCTC CTACATAGCC 
AGGTACCTGG GCAGCTTCCC TGAGGAGGCA GCAGAGGGCC GGGGCCGACC TGAGGGCACG 
TGTGCTGCCT GGCACCGCGG CTGTGCTGAG CGGCTGTGTG CGCACAGCCC CCTGCTGCAG 
CTGGAGCCCG ACGGCGACGA GAAGCCTGAA GTCTATTTCC TGCTGAGGCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_015291822.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147308.1 Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X8, mRNA.

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CloneID	OGa40162
Clone ID Related Accession (Same CDS sequence)	XM_015291822.1 , XM_025153772.1
Accession Version	XM_015291822.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGAA CCTGGACACA AAAGAAGGTG GGAAGAAGTC TCATAGTCGA 
TCATTAATGG GCAACTTTGG TAAACACAAA AAAACAGGGA AGGCAACATC CAAATGTAGC 
ACATCAGATG ATGATGAAAA TCAGCAAAAC TCTTCTGGAA AGGAAGCAGG ACAGCTGCAC 
AGTAGACTGA CTCGTCGAAG GAAAACTGTG AAGCTGTGCC GAGCTCTGTC TGACCTAGTG 
GTGTACACGA ACTCTGTGGC AGCCCAGGAT ATAGTAGATG ATGGATCAAC AGGGAATGTG 
CTGTCATTCA GTGAAACAAG AGCCCACCAA GCAGTGCAGC AGAAGGCTGA ACAGTTCATG 
CTTTACAACC AGAAACAGCT TACAAGGGTT TATCCATCAG CGTATCGGAT TGACTCCAGC 
AATTTCAATC CTTTACCTTA CTGGAATGTT GGTTGCCAGC TAGTGGCACT AAACTACCAG 
TCTGAGGGAC GTATGATGCA ATTAAACGAA GCAAAATTTA GGGTAAATGG CAACTGTGGT 
TACGTCCTCA AACCTCAGCA GATGTGCAAA GGCTCATTCA ACCCCTATTC TTCAGATCCA 
CTTCCTGCTA GTCCTAAGAA GCAGCTCATC CTGAAAATCA TCAGTGGACA GCAGCTGCCT 
AAGCCTCCTG ACTCAATGCT AGGAGACAGA GGAGAGATAA TAGATCCCTT TGTCGAGGTG 
GAAATTATTG GGTTACCTGT TGACTGCTGT AAAGATCAGA CCAGGGTGGT TGATGACAAT 
GGGTTTAATC CTGTTTGGGA GGAAACGCTC ACTTTTACCA TCCATATGCC TGAAATAGCT 
TTGGTTCGAT TTCTTGTTTG GGATCATGAC CCCATTGGGC GAGACTTCGT TGGACAAAGA 
ACGCTGGCCT TTAGCAGTCT TGTACCTGGT TATCGACATG TGTATTTAGA AGGGCTGACA 
GAAGCTTCCA TATTTGTTCA CATAACCATT AATGAAATCT ATGGGAAGTG GAGCCCCTTA 
ATCCTCAATC CCAGTTACAC AATATTGCAC TTTCTAGGAG CCACAAAGAG TAAGCAACTG 
ATCGGACTCC GAGGGCTGTT TAACAAGAAC CCAAAGCACA GTTCTGCTGA AAGCAGCGGT 
CATTATGTAC GGAAGCGTTC TATTGGTGAC CGAATTCTCC GGCGCACGGC CAGTGCTCCA 
GCAAAAGGTA GAAAGAAAAG CAAGATGGGT TTTCTGGAAG CTTCTGAAAT TAAAGACAGT 
GCATCCGAAC CAGCAGACCT GAAGGACAAA GAAGGAGTTG TAAGGCGTAC AAGCCGGAGT 
TTGCAAGCAC GTCCTGCTTC TATGCCAGTG GACAAATTCC TTCTTGTAGG ACTGCCGTGC 
ACAGAAGATG AAACTGCTCA AGATGCCAAA GGAAAAGACA GTGCAACTGC CAACAGTGAT 
GGCGATAAAA CTGAGAAGGA AACAAACTTG AAGGAATCTC TTTTCACGTG TTCTGAGAAA 
ACTGCAGATC ATTCAAACTT GGCATCTCCC TTGAAAAAGG AGAGCGCCCA GAAGGAAACT 
ACAGCTGATT TTTCTCTTGT ACCCCAGGAG CAGCCCGAGC ATTCGGTTGC TGTAAGTGAT 
GTAACCGAAA CAAACCGCCC CATAAGCAAT CAGGAAAGTC TCCTTCCTGC TGAAACGGTG 
CCTCTGATTC CGGACTCTGC CTTTGAGCGC ATCACAGAAA AAATACAGGG CAAGAGCTGT 
GCAGGCAGTA GAAGGGCAGA TGACACAAAC AGGTGTGAGG AGAAAATAAC TCCTGTCAGG 
ACTTGCCTTC CTGAAAAAGT AGAGGAGGTT GAAAATCTCA AATACGACAT CATCCACAGA 
AGCACTGTGG CGTGTCTTTC TGTGACAGAT AGTTCTCCTG CCGTCTACTC TGATGCTCCT 
GGTTTATGCT CCTCTTCAAC CATGCAAGAC AGCGACATCT CCCGCCTCAT AGACGAAGTT 
TCTTTGGTGA CTGAGAGCGA GATGGACAGC GCTGTCTCAG CTCTGATCGG CCAGCTGGAT 
GTCACCGATG ACCAAACCAG CCTCACCGCA CCGATGCTCC CTGGCGCTGG CAGCCGGGCC 
TTCAGTGCTG TGGCCACACG CACACTGACT GCAGAGCTCA GCACTCCCTG TAAGAAGGAC 
TTCATTGCCA CACAGTCCAG CAAGTCCCCG TTATTTTCAA CTCCAGACTC TGCCGTGTTC 
CCCAGCCCCG AGACTGCGGA GTATTCTGTG TACACAATTA TCCACGAGGC AACTCTTACA 
CCCGGTTCTG AATTTAAGAC CCACAGTGGG TTAGTTTGCA AGAAGTCAAA GAGTGCAGAA 
AATAACTTGC CTAGCTCTTT TTGTTCTTCA CCTTTCTCTT TGCTGAATGC AAATCCCCAA 
AGAAAATGTG AAACAAGCTG GGAAGCCCCC GGCTCTGCGG GTTCTTTTGC TTCCAGTAGC 
CTCATCTTTG AGGAGATGCT TGTGGACCCT CCCATGGGCG AGAATTCGGA AAGCAGCAGT 
CTTGTAGAGT TAGGTGGGGA TTCCCAAGAC CTCTTGGTAA CTGCGTGCGC GTACAAAAGG 
GAAGATGTGA GCCAATTGGC TTCCCCTTTA AAACTGAGGC AGCAGCAGGA CAGCAGGTAC 
TGTGGTGAGA GGACCTTTGG AAACTGTGCA AGTGCTGGGC TCTGTGCTGC TGCCCTGGAC 
TGCTCGGATA ACTTTAGGAC TGCGGGAAGC AAGCTCCCTT TTCCAGCCTG TGAAAATGTT 
GGTTTTCTAC ATCATCGTCA CACAGATGAG CAGAAAGATT TGATGTGTAC CTCCAGGAGG 
TCCCAACTCG ATGTGCACTC ACCAGTGCTC AGAAGCGCGT TGGCTTTCCC ACCCTCCCCA 
GCCATCAAAC AGACCAGCCC TTGCAAGTCC AAGAGCCTTG GTGACTTGAC ATCGGAAGAT 
ATTTCCTGCA ATTTTGAAAG TAAGTATCAG TACATAAGTA GGAGCTTTGT CACATCTGGC 
ATGAGAGACA AGAAGCTCGC TGCCATGAAG ACGATGAGAC CGCACTCTAC GGATGCTTTG 
ACGGAACAGC TGAGAAAGCT GCTGTCCCTG GACCAGGACG ACAGCCAGCA GATGCTGTAT 
TCCAGGCGGC TCGACGAGGA CTGCCCAAAG GCACTGGTCA GAAAGCTGTC GTCCAGGAGC 
CAGAGCAGAG TGCGGAACAT CGCCAGCCGT GCCAAGGAGA GGCAGGAGGC CAGCAAGCAA 
AAATCTTCCA CCGCAGGCAG CATAGCGGGG GTCGTCCTTC GGAACAAGCC TTCGGCGTCC 
CCTCACGTCG TCAACAGGCA CTCGACGGGC TCCTACATAG CCAGGTACCT GGGCAGCTTC 
CCTGAGGAGG CAGCAGAGGG CCGGGGCCGA CCTGAGGGCA CGTGTGCTGC CTGGCACCGC 
GGCTGTGCTG AGCGGCTGTG TGCGCACAGC CCCCTGCTGC AGCTGGAGCC CGACGGCGAC 
GAGAAGCCTG AAGTCTATTT CCTGCTGAGG CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147308.1
CDS	1382..6457
Translation	MSYWSLDKKSCLQYCRHFLADNGVFHVERCMSVMQSGTQMVKLKSGTKGLVRLFYLDEHRTCIRWRPSRKSEKAKIVIDSIYKVTEGRQSEIFHRHAEGSFDPSCCFTIYHGNHLESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDHWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLNFEEFSVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTPEYCLDIIQKFEVSEENKEQNVLGIEGFTNFMRSPACDVFNPLHCEVHQDMDQPLCNYFIASSHNTYLTGDQLLSQSRVEMYARVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKIFFRDVAETINKYAFIKNEFPVILSIENHCSIQQQKKIAQYLKEILGDKLDLSSVVTGDSRQLPSPQNLKGKILVKGKKLPYSLGADAEEGEVSDEDSADEIEDDCKLKSCYSNGATEHQVESFIRTKLESLLKESQIRDKEDPDSFTNLDTKEGGKKSHSRSLMGNFGKHKKTGKATSKCSTSDDDENQQNSSGKEAGQLHSRLTRRRKTVKLCRALSDLVVYTNSVAAQDIVDDGSTGNVLSFSETRAHQAVQQKAEQFMLYNQKQLTRVYPSAYRIDSSNFNPLPYWNVGCQLVALNYQSEGRMMQLNEAKFRVNGNCGYVLKPQQMCKGSFNPYSSDPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTIHMPEIALVRFLVWDHDPIGRDFVGQRTLAFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSKQLIGLRGLFNKNPKHSSAESSGHYVRKRSIGDRILRRTASAPAKGRKKSKMGFLEASEIKDSASEPADLKDKEGVVRRTSRSLQARPASMPVDKFLLVGLPCTEDETAQDAKGKDSATANSDGDKTEKETNLKESLFTCSEKTADHSNLASPLKKESAQKETTADFSLVPQEQPEHSVAVSDVTETNRPISNQESLLPAETVPLIPDSAFERITEKIQGKSCAGSRRADDTNRCEEKITPVRTCLPEKVEEVENLKYDIIHRSTVACLSVTDSSPAVYSDAPGLCSSSTMQDSDISRLIDEVSLVTESEMDSAVSALIGQLDVTDDQTSLTAPMLPGAGSRAFSAVATRTLTAELSTPCKKDFIATQSSKSPLFSTPDSAVFPSPETAEYSVYTIIHEATLTPGSEFKTHSGLVCKKSKSAENNLPSSFCSSPFSLLNANPQRKCETSWEAPGSAGSFASSSLIFEEMLVDPPMGENSESSSLVELGGDSQDLLVTACAYKREDVSQLASPLKLRQQQDSRYCGERTFGNCASAGLCAAALDCSDNFRTAGSKLPFPACENVGFLHHRHTDEQKDLMCTSRRSQLDVHSPVLRSALAFPPSPAIKQTSPCKSKSLGDLTSEDISCNFESKYQYISRSFVTSGMRDKKLAAMKTMRPHSTDALTEQLRKLLSLDQDDSQQMLYSRRLDEDCPKALVRKLSSRSQSRVRNIASRAKERQEASKQKSSTAGSIAGVVLRNKPSASPHVVNRHSTGSYIARYLGSFPEEAAEGRGRPEGTCAAWHRGCAERLCAHSPLLQLEPDGDEKPEVYFLLRL

Target ORF information:

RefSeq Version	XM_015291822.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291822.1

ORF Insert Sequence:

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGAA CCTGGACACA AAAGAAGGTG GGAAGAAGTC TCATAGTCGA 
TCATTAATGG GCAACTTTGG TAAACACAAA AAAACAGGGA AGGCAACATC CAAATGTAGC 
ACATCAGATG ATGATGAAAA TCAGCAAAAC TCTTCTGGAA AGGAAGCAGG ACAGCTGCAC 
AGTAGACTGA CTCGTCGAAG GAAAACTGTG AAGCTGTGCC GAGCTCTGTC TGACCTAGTG 
GTGTACACGA ACTCTGTGGC AGCCCAGGAT ATAGTAGATG ATGGATCAAC AGGGAATGTG 
CTGTCATTCA GTGAAACAAG AGCCCACCAA GCAGTGCAGC AGAAGGCTGA ACAGTTCATG 
CTTTACAACC AGAAACAGCT TACAAGGGTT TATCCATCAG CGTATCGGAT TGACTCCAGC 
AATTTCAATC CTTTACCTTA CTGGAATGTT GGTTGCCAGC TAGTGGCACT AAACTACCAG 
TCTGAGGGAC GTATGATGCA ATTAAACGAA GCAAAATTTA GGGTAAATGG CAACTGTGGT 
TACGTCCTCA AACCTCAGCA GATGTGCAAA GGCTCATTCA ACCCCTATTC TTCAGATCCA 
CTTCCTGCTA GTCCTAAGAA GCAGCTCATC CTGAAAATCA TCAGTGGACA GCAGCTGCCT 
AAGCCTCCTG ACTCAATGCT AGGAGACAGA GGAGAGATAA TAGATCCCTT TGTCGAGGTG 
GAAATTATTG GGTTACCTGT TGACTGCTGT AAAGATCAGA CCAGGGTGGT TGATGACAAT 
GGGTTTAATC CTGTTTGGGA GGAAACGCTC ACTTTTACCA TCCATATGCC TGAAATAGCT 
TTGGTTCGAT TTCTTGTTTG GGATCATGAC CCCATTGGGC GAGACTTCGT TGGACAAAGA 
ACGCTGGCCT TTAGCAGTCT TGTACCTGGT TATCGACATG TGTATTTAGA AGGGCTGACA 
GAAGCTTCCA TATTTGTTCA CATAACCATT AATGAAATCT ATGGGAAGTG GAGCCCCTTA 
ATCCTCAATC CCAGTTACAC AATATTGCAC TTTCTAGGAG CCACAAAGAG TAAGCAACTG 
ATCGGACTCC GAGGGCTGTT TAACAAGAAC CCAAAGCACA GTTCTGCTGA AAGCAGCGGT 
CATTATGTAC GGAAGCGTTC TATTGGTGAC CGAATTCTCC GGCGCACGGC CAGTGCTCCA 
GCAAAAGGTA GAAAGAAAAG CAAGATGGGT TTTCTGGAAG CTTCTGAAAT TAAAGACAGT 
GCATCCGAAC CAGCAGACCT GAAGGACAAA GAAGGAGTTG TAAGGCGTAC AAGCCGGAGT 
TTGCAAGCAC GTCCTGCTTC TATGCCAGTG GACAAATTCC TTCTTGTAGG ACTGCCGTGC 
ACAGAAGATG AAACTGCTCA AGATGCCAAA GGAAAAGACA GTGCAACTGC CAACAGTGAT 
GGCGATAAAA CTGAGAAGGA AACAAACTTG AAGGAATCTC TTTTCACGTG TTCTGAGAAA 
ACTGCAGATC ATTCAAACTT GGCATCTCCC TTGAAAAAGG AGAGCGCCCA GAAGGAAACT 
ACAGCTGATT TTTCTCTTGT ACCCCAGGAG CAGCCCGAGC ATTCGGTTGC TGTAAGTGAT 
GTAACCGAAA CAAACCGCCC CATAAGCAAT CAGGAAAGTC TCCTTCCTGC TGAAACGGTG 
CCTCTGATTC CGGACTCTGC CTTTGAGCGC ATCACAGAAA AAATACAGGG CAAGAGCTGT 
GCAGGCAGTA GAAGGGCAGA TGACACAAAC AGGTGTGAGG AGAAAATAAC TCCTGTCAGG 
ACTTGCCTTC CTGAAAAAGT AGAGGAGGTT GAAAATCTCA AATACGACAT CATCCACAGA 
AGCACTGTGG CGTGTCTTTC TGTGACAGAT AGTTCTCCTG CCGTCTACTC TGATGCTCCT 
GGTTTATGCT CCTCTTCAAC CATGCAAGAC AGCGACATCT CCCGCCTCAT AGACGAAGTT 
TCTTTGGTGA CTGAGAGCGA GATGGACAGC GCTGTCTCAG CTCTGATCGG CCAGCTGGAT 
GTCACCGATG ACCAAACCAG CCTCACCGCA CCGATGCTCC CTGGCGCTGG CAGCCGGGCC 
TTCAGTGCTG TGGCCACACG CACACTGACT GCAGAGCTCA GCACTCCCTG TAAGAAGGAC 
TTCATTGCCA CACAGTCCAG CAAGTCCCCG TTATTTTCAA CTCCAGACTC TGCCGTGTTC 
CCCAGCCCCG AGACTGCGGA GTATTCTGTG TACACAATTA TCCACGAGGC AACTCTTACA 
CCCGGTTCTG AATTTAAGAC CCACAGTGGG TTAGTTTGCA AGAAGTCAAA GAGTGCAGAA 
AATAACTTGC CTAGCTCTTT TTGTTCTTCA CCTTTCTCTT TGCTGAATGC AAATCCCCAA 
AGAAAATGTG AAACAAGCTG GGAAGCCCCC GGCTCTGCGG GTTCTTTTGC TTCCAGTAGC 
CTCATCTTTG AGGAGATGCT TGTGGACCCT CCCATGGGCG AGAATTCGGA AAGCAGCAGT 
CTTGTAGAGT TAGGTGGGGA TTCCCAAGAC CTCTTGGTAA CTGCGTGCGC GTACAAAAGG 
GAAGATGTGA GCCAATTGGC TTCCCCTTTA AAACTGAGGC AGCAGCAGGA CAGCAGGTAC 
TGTGGTGAGA GGACCTTTGG AAACTGTGCA AGTGCTGGGC TCTGTGCTGC TGCCCTGGAC 
TGCTCGGATA ACTTTAGGAC TGCGGGAAGC AAGCTCCCTT TTCCAGCCTG TGAAAATGTT 
GGTTTTCTAC ATCATCGTCA CACAGATGAG CAGAAAGATT TGATGTGTAC CTCCAGGAGG 
TCCCAACTCG ATGTGCACTC ACCAGTGCTC AGAAGCGCGT TGGCTTTCCC ACCCTCCCCA 
GCCATCAAAC AGACCAGCCC TTGCAAGTCC AAGAGCCTTG GTGACTTGAC ATCGGAAGAT 
ATTTCCTGCA ATTTTGAAAG TAAGTATCAG TACATAAGTA GGAGCTTTGT CACATCTGGC 
ATGAGAGACA AGAAGCTCGC TGCCATGAAG ACGATGAGAC CGCACTCTAC GGATGCTTTG 
ACGGAACAGC TGAGAAAGCT GCTGTCCCTG GACCAGGACG ACAGCCAGCA GATGCTGTAT 
TCCAGGCGGC TCGACGAGGA CTGCCCAAAG GCACTGGTCA GAAAGCTGTC GTCCAGGAGC 
CAGAGCAGAG TGCGGAACAT CGCCAGCCGT GCCAAGGAGA GGCAGGAGGC CAGCAAGCAA 
AAATCTTCCA CCGCAGGCAG CATAGCGGGG GTCGTCCTTC GGAACAAGCC TTCGGCGTCC 
CCTCACGTCG TCAACAGGCA CTCGACGGGC TCCTACATAG CCAGGTACCT GGGCAGCTTC 
CCTGAGGAGG CAGCAGAGGG CCGGGGCCGA CCTGAGGGCA CGTGTGCTGC CTGGCACCGC 
GGCTGTGCTG AGCGGCTGTG TGCGCACAGC CCCCTGCTGC AGCTGGAGCC CGACGGCGAC 
GAGAAGCCTG AAGTCTATTT CCTGCTGAGG CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_025153772.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025009540.1 Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X10, mRNA.

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CloneID	OGa40162
Clone ID Related Accession (Same CDS sequence)	XM_015291822.1 , XM_025153772.1
Accession Version	XM_025153772.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGAA CCTGGACACA AAAGAAGGTG GGAAGAAGTC TCATAGTCGA 
TCATTAATGG GCAACTTTGG TAAACACAAA AAAACAGGGA AGGCAACATC CAAATGTAGC 
ACATCAGATG ATGATGAAAA TCAGCAAAAC TCTTCTGGAA AGGAAGCAGG ACAGCTGCAC 
AGTAGACTGA CTCGTCGAAG GAAAACTGTG AAGCTGTGCC GAGCTCTGTC TGACCTAGTG 
GTGTACACGA ACTCTGTGGC AGCCCAGGAT ATAGTAGATG ATGGATCAAC AGGGAATGTG 
CTGTCATTCA GTGAAACAAG AGCCCACCAA GCAGTGCAGC AGAAGGCTGA ACAGTTCATG 
CTTTACAACC AGAAACAGCT TACAAGGGTT TATCCATCAG CGTATCGGAT TGACTCCAGC 
AATTTCAATC CTTTACCTTA CTGGAATGTT GGTTGCCAGC TAGTGGCACT AAACTACCAG 
TCTGAGGGAC GTATGATGCA ATTAAACGAA GCAAAATTTA GGGTAAATGG CAACTGTGGT 
TACGTCCTCA AACCTCAGCA GATGTGCAAA GGCTCATTCA ACCCCTATTC TTCAGATCCA 
CTTCCTGCTA GTCCTAAGAA GCAGCTCATC CTGAAAATCA TCAGTGGACA GCAGCTGCCT 
AAGCCTCCTG ACTCAATGCT AGGAGACAGA GGAGAGATAA TAGATCCCTT TGTCGAGGTG 
GAAATTATTG GGTTACCTGT TGACTGCTGT AAAGATCAGA CCAGGGTGGT TGATGACAAT 
GGGTTTAATC CTGTTTGGGA GGAAACGCTC ACTTTTACCA TCCATATGCC TGAAATAGCT 
TTGGTTCGAT TTCTTGTTTG GGATCATGAC CCCATTGGGC GAGACTTCGT TGGACAAAGA 
ACGCTGGCCT TTAGCAGTCT TGTACCTGGT TATCGACATG TGTATTTAGA AGGGCTGACA 
GAAGCTTCCA TATTTGTTCA CATAACCATT AATGAAATCT ATGGGAAGTG GAGCCCCTTA 
ATCCTCAATC CCAGTTACAC AATATTGCAC TTTCTAGGAG CCACAAAGAG TAAGCAACTG 
ATCGGACTCC GAGGGCTGTT TAACAAGAAC CCAAAGCACA GTTCTGCTGA AAGCAGCGGT 
CATTATGTAC GGAAGCGTTC TATTGGTGAC CGAATTCTCC GGCGCACGGC CAGTGCTCCA 
GCAAAAGGTA GAAAGAAAAG CAAGATGGGT TTTCTGGAAG CTTCTGAAAT TAAAGACAGT 
GCATCCGAAC CAGCAGACCT GAAGGACAAA GAAGGAGTTG TAAGGCGTAC AAGCCGGAGT 
TTGCAAGCAC GTCCTGCTTC TATGCCAGTG GACAAATTCC TTCTTGTAGG ACTGCCGTGC 
ACAGAAGATG AAACTGCTCA AGATGCCAAA GGAAAAGACA GTGCAACTGC CAACAGTGAT 
GGCGATAAAA CTGAGAAGGA AACAAACTTG AAGGAATCTC TTTTCACGTG TTCTGAGAAA 
ACTGCAGATC ATTCAAACTT GGCATCTCCC TTGAAAAAGG AGAGCGCCCA GAAGGAAACT 
ACAGCTGATT TTTCTCTTGT ACCCCAGGAG CAGCCCGAGC ATTCGGTTGC TGTAAGTGAT 
GTAACCGAAA CAAACCGCCC CATAAGCAAT CAGGAAAGTC TCCTTCCTGC TGAAACGGTG 
CCTCTGATTC CGGACTCTGC CTTTGAGCGC ATCACAGAAA AAATACAGGG CAAGAGCTGT 
GCAGGCAGTA GAAGGGCAGA TGACACAAAC AGGTGTGAGG AGAAAATAAC TCCTGTCAGG 
ACTTGCCTTC CTGAAAAAGT AGAGGAGGTT GAAAATCTCA AATACGACAT CATCCACAGA 
AGCACTGTGG CGTGTCTTTC TGTGACAGAT AGTTCTCCTG CCGTCTACTC TGATGCTCCT 
GGTTTATGCT CCTCTTCAAC CATGCAAGAC AGCGACATCT CCCGCCTCAT AGACGAAGTT 
TCTTTGGTGA CTGAGAGCGA GATGGACAGC GCTGTCTCAG CTCTGATCGG CCAGCTGGAT 
GTCACCGATG ACCAAACCAG CCTCACCGCA CCGATGCTCC CTGGCGCTGG CAGCCGGGCC 
TTCAGTGCTG TGGCCACACG CACACTGACT GCAGAGCTCA GCACTCCCTG TAAGAAGGAC 
TTCATTGCCA CACAGTCCAG CAAGTCCCCG TTATTTTCAA CTCCAGACTC TGCCGTGTTC 
CCCAGCCCCG AGACTGCGGA GTATTCTGTG TACACAATTA TCCACGAGGC AACTCTTACA 
CCCGGTTCTG AATTTAAGAC CCACAGTGGG TTAGTTTGCA AGAAGTCAAA GAGTGCAGAA 
AATAACTTGC CTAGCTCTTT TTGTTCTTCA CCTTTCTCTT TGCTGAATGC AAATCCCCAA 
AGAAAATGTG AAACAAGCTG GGAAGCCCCC GGCTCTGCGG GTTCTTTTGC TTCCAGTAGC 
CTCATCTTTG AGGAGATGCT TGTGGACCCT CCCATGGGCG AGAATTCGGA AAGCAGCAGT 
CTTGTAGAGT TAGGTGGGGA TTCCCAAGAC CTCTTGGTAA CTGCGTGCGC GTACAAAAGG 
GAAGATGTGA GCCAATTGGC TTCCCCTTTA AAACTGAGGC AGCAGCAGGA CAGCAGGTAC 
TGTGGTGAGA GGACCTTTGG AAACTGTGCA AGTGCTGGGC TCTGTGCTGC TGCCCTGGAC 
TGCTCGGATA ACTTTAGGAC TGCGGGAAGC AAGCTCCCTT TTCCAGCCTG TGAAAATGTT 
GGTTTTCTAC ATCATCGTCA CACAGATGAG CAGAAAGATT TGATGTGTAC CTCCAGGAGG 
TCCCAACTCG ATGTGCACTC ACCAGTGCTC AGAAGCGCGT TGGCTTTCCC ACCCTCCCCA 
GCCATCAAAC AGACCAGCCC TTGCAAGTCC AAGAGCCTTG GTGACTTGAC ATCGGAAGAT 
ATTTCCTGCA ATTTTGAAAG TAAGTATCAG TACATAAGTA GGAGCTTTGT CACATCTGGC 
ATGAGAGACA AGAAGCTCGC TGCCATGAAG ACGATGAGAC CGCACTCTAC GGATGCTTTG 
ACGGAACAGC TGAGAAAGCT GCTGTCCCTG GACCAGGACG ACAGCCAGCA GATGCTGTAT 
TCCAGGCGGC TCGACGAGGA CTGCCCAAAG GCACTGGTCA GAAAGCTGTC GTCCAGGAGC 
CAGAGCAGAG TGCGGAACAT CGCCAGCCGT GCCAAGGAGA GGCAGGAGGC CAGCAAGCAA 
AAATCTTCCA CCGCAGGCAG CATAGCGGGG GTCGTCCTTC GGAACAAGCC TTCGGCGTCC 
CCTCACGTCG TCAACAGGCA CTCGACGGGC TCCTACATAG CCAGGTACCT GGGCAGCTTC 
CCTGAGGAGG CAGCAGAGGG CCGGGGCCGA CCTGAGGGCA CGTGTGCTGC CTGGCACCGC 
GGCTGTGCTG AGCGGCTGTG TGCGCACAGC CCCCTGCTGC AGCTGGAGCC CGACGGCGAC 
GAGAAGCCTG AAGTCTATTT CCTGCTGAGG CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025009540.1
CDS	339..5414
Translation	MSYWSLDKKSCLQYCRHFLADNGVFHVERCMSVMQSGTQMVKLKSGTKGLVRLFYLDEHRTCIRWRPSRKSEKAKIVIDSIYKVTEGRQSEIFHRHAEGSFDPSCCFTIYHGNHLESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDHWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLNFEEFSVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTPEYCLDIIQKFEVSEENKEQNVLGIEGFTNFMRSPACDVFNPLHCEVHQDMDQPLCNYFIASSHNTYLTGDQLLSQSRVEMYARVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKIFFRDVAETINKYAFIKNEFPVILSIENHCSIQQQKKIAQYLKEILGDKLDLSSVVTGDSRQLPSPQNLKGKILVKGKKLPYSLGADAEEGEVSDEDSADEIEDDCKLKSCYSNGATEHQVESFIRTKLESLLKESQIRDKEDPDSFTNLDTKEGGKKSHSRSLMGNFGKHKKTGKATSKCSTSDDDENQQNSSGKEAGQLHSRLTRRRKTVKLCRALSDLVVYTNSVAAQDIVDDGSTGNVLSFSETRAHQAVQQKAEQFMLYNQKQLTRVYPSAYRIDSSNFNPLPYWNVGCQLVALNYQSEGRMMQLNEAKFRVNGNCGYVLKPQQMCKGSFNPYSSDPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTIHMPEIALVRFLVWDHDPIGRDFVGQRTLAFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSKQLIGLRGLFNKNPKHSSAESSGHYVRKRSIGDRILRRTASAPAKGRKKSKMGFLEASEIKDSASEPADLKDKEGVVRRTSRSLQARPASMPVDKFLLVGLPCTEDETAQDAKGKDSATANSDGDKTEKETNLKESLFTCSEKTADHSNLASPLKKESAQKETTADFSLVPQEQPEHSVAVSDVTETNRPISNQESLLPAETVPLIPDSAFERITEKIQGKSCAGSRRADDTNRCEEKITPVRTCLPEKVEEVENLKYDIIHRSTVACLSVTDSSPAVYSDAPGLCSSSTMQDSDISRLIDEVSLVTESEMDSAVSALIGQLDVTDDQTSLTAPMLPGAGSRAFSAVATRTLTAELSTPCKKDFIATQSSKSPLFSTPDSAVFPSPETAEYSVYTIIHEATLTPGSEFKTHSGLVCKKSKSAENNLPSSFCSSPFSLLNANPQRKCETSWEAPGSAGSFASSSLIFEEMLVDPPMGENSESSSLVELGGDSQDLLVTACAYKREDVSQLASPLKLRQQQDSRYCGERTFGNCASAGLCAAALDCSDNFRTAGSKLPFPACENVGFLHHRHTDEQKDLMCTSRRSQLDVHSPVLRSALAFPPSPAIKQTSPCKSKSLGDLTSEDISCNFESKYQYISRSFVTSGMRDKKLAAMKTMRPHSTDALTEQLRKLLSLDQDDSQQMLYSRRLDEDCPKALVRKLSSRSQSRVRNIASRAKERQEASKQKSSTAGSIAGVVLRNKPSASPHVVNRHSTGSYIARYLGSFPEEAAEGRGRPEGTCAAWHRGCAERLCAHSPLLQLEPDGDEKPEVYFLLRL

Target ORF information:

RefSeq Version	XM_025153772.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025153772.1

ORF Insert Sequence:

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGAA CCTGGACACA AAAGAAGGTG GGAAGAAGTC TCATAGTCGA 
TCATTAATGG GCAACTTTGG TAAACACAAA AAAACAGGGA AGGCAACATC CAAATGTAGC 
ACATCAGATG ATGATGAAAA TCAGCAAAAC TCTTCTGGAA AGGAAGCAGG ACAGCTGCAC 
AGTAGACTGA CTCGTCGAAG GAAAACTGTG AAGCTGTGCC GAGCTCTGTC TGACCTAGTG 
GTGTACACGA ACTCTGTGGC AGCCCAGGAT ATAGTAGATG ATGGATCAAC AGGGAATGTG 
CTGTCATTCA GTGAAACAAG AGCCCACCAA GCAGTGCAGC AGAAGGCTGA ACAGTTCATG 
CTTTACAACC AGAAACAGCT TACAAGGGTT TATCCATCAG CGTATCGGAT TGACTCCAGC 
AATTTCAATC CTTTACCTTA CTGGAATGTT GGTTGCCAGC TAGTGGCACT AAACTACCAG 
TCTGAGGGAC GTATGATGCA ATTAAACGAA GCAAAATTTA GGGTAAATGG CAACTGTGGT 
TACGTCCTCA AACCTCAGCA GATGTGCAAA GGCTCATTCA ACCCCTATTC TTCAGATCCA 
CTTCCTGCTA GTCCTAAGAA GCAGCTCATC CTGAAAATCA TCAGTGGACA GCAGCTGCCT 
AAGCCTCCTG ACTCAATGCT AGGAGACAGA GGAGAGATAA TAGATCCCTT TGTCGAGGTG 
GAAATTATTG GGTTACCTGT TGACTGCTGT AAAGATCAGA CCAGGGTGGT TGATGACAAT 
GGGTTTAATC CTGTTTGGGA GGAAACGCTC ACTTTTACCA TCCATATGCC TGAAATAGCT 
TTGGTTCGAT TTCTTGTTTG GGATCATGAC CCCATTGGGC GAGACTTCGT TGGACAAAGA 
ACGCTGGCCT TTAGCAGTCT TGTACCTGGT TATCGACATG TGTATTTAGA AGGGCTGACA 
GAAGCTTCCA TATTTGTTCA CATAACCATT AATGAAATCT ATGGGAAGTG GAGCCCCTTA 
ATCCTCAATC CCAGTTACAC AATATTGCAC TTTCTAGGAG CCACAAAGAG TAAGCAACTG 
ATCGGACTCC GAGGGCTGTT TAACAAGAAC CCAAAGCACA GTTCTGCTGA AAGCAGCGGT 
CATTATGTAC GGAAGCGTTC TATTGGTGAC CGAATTCTCC GGCGCACGGC CAGTGCTCCA 
GCAAAAGGTA GAAAGAAAAG CAAGATGGGT TTTCTGGAAG CTTCTGAAAT TAAAGACAGT 
GCATCCGAAC CAGCAGACCT GAAGGACAAA GAAGGAGTTG TAAGGCGTAC AAGCCGGAGT 
TTGCAAGCAC GTCCTGCTTC TATGCCAGTG GACAAATTCC TTCTTGTAGG ACTGCCGTGC 
ACAGAAGATG AAACTGCTCA AGATGCCAAA GGAAAAGACA GTGCAACTGC CAACAGTGAT 
GGCGATAAAA CTGAGAAGGA AACAAACTTG AAGGAATCTC TTTTCACGTG TTCTGAGAAA 
ACTGCAGATC ATTCAAACTT GGCATCTCCC TTGAAAAAGG AGAGCGCCCA GAAGGAAACT 
ACAGCTGATT TTTCTCTTGT ACCCCAGGAG CAGCCCGAGC ATTCGGTTGC TGTAAGTGAT 
GTAACCGAAA CAAACCGCCC CATAAGCAAT CAGGAAAGTC TCCTTCCTGC TGAAACGGTG 
CCTCTGATTC CGGACTCTGC CTTTGAGCGC ATCACAGAAA AAATACAGGG CAAGAGCTGT 
GCAGGCAGTA GAAGGGCAGA TGACACAAAC AGGTGTGAGG AGAAAATAAC TCCTGTCAGG 
ACTTGCCTTC CTGAAAAAGT AGAGGAGGTT GAAAATCTCA AATACGACAT CATCCACAGA 
AGCACTGTGG CGTGTCTTTC TGTGACAGAT AGTTCTCCTG CCGTCTACTC TGATGCTCCT 
GGTTTATGCT CCTCTTCAAC CATGCAAGAC AGCGACATCT CCCGCCTCAT AGACGAAGTT 
TCTTTGGTGA CTGAGAGCGA GATGGACAGC GCTGTCTCAG CTCTGATCGG CCAGCTGGAT 
GTCACCGATG ACCAAACCAG CCTCACCGCA CCGATGCTCC CTGGCGCTGG CAGCCGGGCC 
TTCAGTGCTG TGGCCACACG CACACTGACT GCAGAGCTCA GCACTCCCTG TAAGAAGGAC 
TTCATTGCCA CACAGTCCAG CAAGTCCCCG TTATTTTCAA CTCCAGACTC TGCCGTGTTC 
CCCAGCCCCG AGACTGCGGA GTATTCTGTG TACACAATTA TCCACGAGGC AACTCTTACA 
CCCGGTTCTG AATTTAAGAC CCACAGTGGG TTAGTTTGCA AGAAGTCAAA GAGTGCAGAA 
AATAACTTGC CTAGCTCTTT TTGTTCTTCA CCTTTCTCTT TGCTGAATGC AAATCCCCAA 
AGAAAATGTG AAACAAGCTG GGAAGCCCCC GGCTCTGCGG GTTCTTTTGC TTCCAGTAGC 
CTCATCTTTG AGGAGATGCT TGTGGACCCT CCCATGGGCG AGAATTCGGA AAGCAGCAGT 
CTTGTAGAGT TAGGTGGGGA TTCCCAAGAC CTCTTGGTAA CTGCGTGCGC GTACAAAAGG 
GAAGATGTGA GCCAATTGGC TTCCCCTTTA AAACTGAGGC AGCAGCAGGA CAGCAGGTAC 
TGTGGTGAGA GGACCTTTGG AAACTGTGCA AGTGCTGGGC TCTGTGCTGC TGCCCTGGAC 
TGCTCGGATA ACTTTAGGAC TGCGGGAAGC AAGCTCCCTT TTCCAGCCTG TGAAAATGTT 
GGTTTTCTAC ATCATCGTCA CACAGATGAG CAGAAAGATT TGATGTGTAC CTCCAGGAGG 
TCCCAACTCG ATGTGCACTC ACCAGTGCTC AGAAGCGCGT TGGCTTTCCC ACCCTCCCCA 
GCCATCAAAC AGACCAGCCC TTGCAAGTCC AAGAGCCTTG GTGACTTGAC ATCGGAAGAT 
ATTTCCTGCA ATTTTGAAAG TAAGTATCAG TACATAAGTA GGAGCTTTGT CACATCTGGC 
ATGAGAGACA AGAAGCTCGC TGCCATGAAG ACGATGAGAC CGCACTCTAC GGATGCTTTG 
ACGGAACAGC TGAGAAAGCT GCTGTCCCTG GACCAGGACG ACAGCCAGCA GATGCTGTAT 
TCCAGGCGGC TCGACGAGGA CTGCCCAAAG GCACTGGTCA GAAAGCTGTC GTCCAGGAGC 
CAGAGCAGAG TGCGGAACAT CGCCAGCCGT GCCAAGGAGA GGCAGGAGGC CAGCAAGCAA 
AAATCTTCCA CCGCAGGCAG CATAGCGGGG GTCGTCCTTC GGAACAAGCC TTCGGCGTCC 
CCTCACGTCG TCAACAGGCA CTCGACGGGC TCCTACATAG CCAGGTACCT GGGCAGCTTC 
CCTGAGGAGG CAGCAGAGGG CCGGGGCCGA CCTGAGGGCA CGTGTGCTGC CTGGCACCGC 
GGCTGTGCTG AGCGGCTGTG TGCGCACAGC CCCCTGCTGC AGCTGGAGCC CGACGGCGAC 
GAGAAGCCTG AAGTCTATTT CCTGCTGAGG CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_025153773.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025009541.1 Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OGa12866
Clone ID Related Accession (Same CDS sequence)	XM_025153773.1 , XM_015291823.1
Accession Version	XM_025153773.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGTAGA 
CTGACTCGTC GAAGGAAAAC TGTGAAGCTG TGCCGAGCTC TGTCTGACCT AGTGGTGTAC 
ACGAACTCTG TGGCAGCCCA GGATATAGTA GATGATGGAT CAACAGGGAA TGTGCTGTCA 
TTCAGTGAAA CAAGAGCCCA CCAAGCAGTG CAGCAGAAGG CTGAACAGTT CATGCTTTAC 
AACCAGAAAC AGCTTACAAG GGTTTATCCA TCAGCGTATC GGATTGACTC CAGCAATTTC 
AATCCTTTAC CTTACTGGAA TGTTGGTTGC CAGCTAGTGG CACTAAACTA CCAGTCTGAG 
GGACGTATGA TGCAATTAAA CGAAGCAAAA TTTAGGGTAA ATGGCAACTG TGGTTACGTC 
CTCAAACCTC AGCAGATGTG CAAAGGCTCA TTCAACCCCT ATTCTTCAGA TCCACTTCCT 
GCTAGTCCTA AGAAGCAGCT CATCCTGAAA ATCATCAGTG GACAGCAGCT GCCTAAGCCT 
CCTGACTCAA TGCTAGGAGA CAGAGGAGAG ATAATAGATC CCTTTGTCGA GGTGGAAATT 
ATTGGGTTAC CTGTTGACTG CTGTAAAGAT CAGACCAGGG TGGTTGATGA CAATGGGTTT 
AATCCTGTTT GGGAGGAAAC GCTCACTTTT ACCATCCATA TGCCTGAAAT AGCTTTGGTT 
CGATTTCTTG TTTGGGATCA TGACCCCATT GGGCGAGACT TCGTTGGACA AAGAACGCTG 
GCCTTTAGCA GTCTTGTACC TGGTTATCGA CATGTGTATT TAGAAGGGCT GACAGAAGCT 
TCCATATTTG TTCACATAAC CATTAATGAA ATCTATGGGA AGAGTAAGCA ACTGATCGGA 
CTCCGAGGGC TGTTTAACAA GAACCCAAAG CACAGTTCTG CTGAAAGCAG CGGTCATTAT 
GTACGGAAGC GTTCTATTGG TGACCGAATT CTCCGGCGCA CGGCCAGTGC TCCAGCAAAA 
GGTAGAAAGA AAAGCAAGAT GGGTTTTCTG GAAGCTTCTG AAATTAAAGA CAGTGCATCC 
GAACCAGCAG ACCTGAAGGA CAAAGAAGGA GTTGTAAGGC GTACAAGCCG GAGTTTGCAA 
GCACGTCCTG CTTCTATGCC AGTGGACAAA TTCCTTCTTG TAGGACTGCC GTGCACAGAA 
GATGAAACTG CTCAAGATGC CAAAGGAAAA GACAGTGCAA CTGCCAACAG TGATGGCGAT 
AAAACTGAGA AGGAAACAAA CTTGAAGGAA TCTCTTTTCA CGTGTTCTGA GAAAACTGCA 
GATCATTCAA ACTTGGCATC TCCCTTGAAA AAGGAGAGCG CCCAGAAGGA AACTACAGCT 
GATTTTTCTC TTGTACCCCA GGAGCAGCCC GAGCATTCGG TTGCTGTAAG TGATGTAACC 
GAAACAAACC GCCCCATAAG CAATCAGGAA AGTCTCCTTC CTGCTGAAAC GGTGCCTCTG 
ATTCCGGACT CTGCCTTTGA GCGCATCACA GAAAAAATAC AGGGCAAGAG CTGTGCAGGC 
AGTAGAAGGG CAGATGACAC AAACAGGTGT GAGGAGAAAA TAACTCCTGT CAGGACTTGC 
CTTCCTGAAA AAGTAGAGGA GGTTGAAAAT CTCAAATACG ACATCATCCA CAGAAGCACT 
GTGGCGTGTC TTTCTGTGAC AGATAGTTCT CCTGCCGTCT ACTCTGATGC TCCTGGTTTA 
TGCTCCTCTT CAACCATGCA AGACAGCGAC ATCTCCCGCC TCATAGACGA AGTTTCTTTG 
GTGACTGAGA GCGAGATGGA CAGCGCTGTC TCAGCTCTGA TCGGCCAGCT GGATGTCACC 
GATGACCAAA CCAGCCTCAC CGCACCGATG CTCCCTGGCG CTGGCAGCCG GGCCTTCAGT 
GCTGTGGCCA CACGCACACT GACTGCAGAG CTCAGCACTC CCTGTAAGAA GGACTTCATT 
GCCACACAGT CCAGCAAGTC CCCGTTATTT TCAACTCCAG ACTCTGCCGT GTTCCCCAGC 
CCCGAGACTG CGGAGTATTC TGTGTACACA ATTATCCACG AGGCAACTCT TACACCCGGT 
TCTGAATTTA AGACCCACAG TGGGTTAGTT TGCAAGAAGT CAAAGAGTGC AGAAAATAAC 
TTGCCTAGCT CTTTTTGTTC TTCACCTTTC TCTTTGCTGA ATGCAAATCC CCAAAGAAAA 
TGTGAAACAA GCTGGGAAGC CCCCGGCTCT GCGGGTTCTT TTGCTTCCAG TAGCCTCATC 
TTTGAGGAGA TGCTTGTGGA CCCTCCCATG GGCGAGAATT CGGAAAGCAG CAGTCTTGTA 
GAGTTAGGTG GGGATTCCCA AGACCTCTTG GTAACTGCGT GCGCGTACAA AAGGGAAGAT 
GTGAGCCAAT TGGCTTCCCC TTTAAAACTG AGGCAGCAGC AGGACAGCAG GTACTGTGGT 
GAGAGGACCT TTGGAAACTG TGCAAGTGCT GGGCTCTGTG CTGCTGCCCT GGACTGCTCG 
GATAACTTTA GGACTGCGGG AAGCAAGCTC CCTTTTCCAG CCTGTGAAAA TGTTGGTTTT 
CTACATCATC GTCACACAGA TGAGCAGAAA GATTTGATGT GTACCTCCAG GAGGTCCCAA 
CTCGATGTGC ACTCACCAGT GCTCAGAAGC GCGTTGGCTT TCCCACCCTC CCCAGCCATC 
AAACAGACCA GCCCTTGCAA GTCCAAGAGC CTTGGTGACT TGACATCGGA AGATATTTCC 
TGCAATTTTG AAAGTAAGTA TCAGTACATA AGTAGGAGCT TTGTCACATC TGGCATGAGA 
GACAAGAAGC TCGCTGCCAT GAAGACGATG AGACCGCACT CTACGGATGC TTTGACGGAA 
CAGCTGAGAA AGCTGCTGTC CCTGGACCAG GACGACAGCC AGCAGATGCT GTATTCCAGG 
CGGCTCGACG AGGACTGCCC AAAGGCACTG GTCAGAAAGC TGTCGTCCAG GAGCCAGAGC 
AGAGTGCGGA ACATCGCCAG CCGTGCCAAG GAGAGGCAGG AGGCCAGCAA GCAAAAATCT 
TCCACCGCAG GCAGCATAGC GGGGGTCGTC CTTCGGAACA AGCCTTCGGC GTCCCCTCAC 
GTCGTCAACA GGCACTCGAC GGGCTCCTAC ATAGCCAGGT ACCTGGGCAG CTTCCCTGAG 
GAGGCAGCAG AGGGCCGGGG CCGACCTGAG GGCACGTGTG CTGCCTGGCA CCGCGGCTGT 
GCTGAGCGGC TGTGTGCGCA CAGCCCCCTG CTGCAGCTGG AGCCCGACGG CGACGAGAAG 
CCTGAAGTCT ATTTCCTGCT GAGGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025009541.1
CDS	337..5406
Translation	MSYWSLDKKSCLQYCRHFLADNGVFHVERCMSVMQSGTQMVKLKSGTKGLVRLFYLDEHRTCIRWRPSRKSEKAKIVIDSIYKVTEGRQSEIFHRHAEGSFDPSCCFTIYHGNHLESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDHWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLNFEEFSVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTPEYCLDIIQKFEVSEENKEQNVLGIEGFTNFMRSPACDVFNPLHCEVHQDMDQPLCNYFIASSHNTYLTGDQLLSQSRVEMYARVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKIFFRDVAETINKYAFIKNEFPVILSIENHCSIQQQKKIAQYLKEILGDKLDLSSVVTGDSRQLPSPQNLKGKILVKGKKLPYSLGADAEEGEVSDEDSADEIEDDCKLKSCYSNGATEHQVESFIRTKLESLLKESQIRDKEDPDSFTVRALLRATHEGLSVNLKQNLDTKEGGKKSHSRSLMGNFGKHKKTGKATSKCSTSDDDENQQNSSGKEAGQLHSRLTRRRKTVKLCRALSDLVVYTNSVAAQDIVDDGSTGNVLSFSETRAHQAVQQKAEQFMLYNQKQLTRVYPSAYRIDSSNFNPLPYWNVGCQLVALNYQSEGRMMQLNEAKFRVNGNCGYVLKPQQMCKGSFNPYSSDPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTIHMPEIALVRFLVWDHDPIGRDFVGQRTLAFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKSKQLIGLRGLFNKNPKHSSAESSGHYVRKRSIGDRILRRTASAPAKGRKKSKMGFLEASEIKDSASEPADLKDKEGVVRRTSRSLQARPASMPVDKFLLVGLPCTEDETAQDAKGKDSATANSDGDKTEKETNLKESLFTCSEKTADHSNLASPLKKESAQKETTADFSLVPQEQPEHSVAVSDVTETNRPISNQESLLPAETVPLIPDSAFERITEKIQGKSCAGSRRADDTNRCEEKITPVRTCLPEKVEEVENLKYDIIHRSTVACLSVTDSSPAVYSDAPGLCSSSTMQDSDISRLIDEVSLVTESEMDSAVSALIGQLDVTDDQTSLTAPMLPGAGSRAFSAVATRTLTAELSTPCKKDFIATQSSKSPLFSTPDSAVFPSPETAEYSVYTIIHEATLTPGSEFKTHSGLVCKKSKSAENNLPSSFCSSPFSLLNANPQRKCETSWEAPGSAGSFASSSLIFEEMLVDPPMGENSESSSLVELGGDSQDLLVTACAYKREDVSQLASPLKLRQQQDSRYCGERTFGNCASAGLCAAALDCSDNFRTAGSKLPFPACENVGFLHHRHTDEQKDLMCTSRRSQLDVHSPVLRSALAFPPSPAIKQTSPCKSKSLGDLTSEDISCNFESKYQYISRSFVTSGMRDKKLAAMKTMRPHSTDALTEQLRKLLSLDQDDSQQMLYSRRLDEDCPKALVRKLSSRSQSRVRNIASRAKERQEASKQKSSTAGSIAGVVLRNKPSASPHVVNRHSTGSYIARYLGSFPEEAAEGRGRPEGTCAAWHRGCAERLCAHSPLLQLEPDGDEKPEVYFLLRL

Target ORF information:

RefSeq Version	XM_025153773.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025153773.1

ORF Insert Sequence:

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGTAGA 
CTGACTCGTC GAAGGAAAAC TGTGAAGCTG TGCCGAGCTC TGTCTGACCT AGTGGTGTAC 
ACGAACTCTG TGGCAGCCCA GGATATAGTA GATGATGGAT CAACAGGGAA TGTGCTGTCA 
TTCAGTGAAA CAAGAGCCCA CCAAGCAGTG CAGCAGAAGG CTGAACAGTT CATGCTTTAC 
AACCAGAAAC AGCTTACAAG GGTTTATCCA TCAGCGTATC GGATTGACTC CAGCAATTTC 
AATCCTTTAC CTTACTGGAA TGTTGGTTGC CAGCTAGTGG CACTAAACTA CCAGTCTGAG 
GGACGTATGA TGCAATTAAA CGAAGCAAAA TTTAGGGTAA ATGGCAACTG TGGTTACGTC 
CTCAAACCTC AGCAGATGTG CAAAGGCTCA TTCAACCCCT ATTCTTCAGA TCCACTTCCT 
GCTAGTCCTA AGAAGCAGCT CATCCTGAAA ATCATCAGTG GACAGCAGCT GCCTAAGCCT 
CCTGACTCAA TGCTAGGAGA CAGAGGAGAG ATAATAGATC CCTTTGTCGA GGTGGAAATT 
ATTGGGTTAC CTGTTGACTG CTGTAAAGAT CAGACCAGGG TGGTTGATGA CAATGGGTTT 
AATCCTGTTT GGGAGGAAAC GCTCACTTTT ACCATCCATA TGCCTGAAAT AGCTTTGGTT 
CGATTTCTTG TTTGGGATCA TGACCCCATT GGGCGAGACT TCGTTGGACA AAGAACGCTG 
GCCTTTAGCA GTCTTGTACC TGGTTATCGA CATGTGTATT TAGAAGGGCT GACAGAAGCT 
TCCATATTTG TTCACATAAC CATTAATGAA ATCTATGGGA AGAGTAAGCA ACTGATCGGA 
CTCCGAGGGC TGTTTAACAA GAACCCAAAG CACAGTTCTG CTGAAAGCAG CGGTCATTAT 
GTACGGAAGC GTTCTATTGG TGACCGAATT CTCCGGCGCA CGGCCAGTGC TCCAGCAAAA 
GGTAGAAAGA AAAGCAAGAT GGGTTTTCTG GAAGCTTCTG AAATTAAAGA CAGTGCATCC 
GAACCAGCAG ACCTGAAGGA CAAAGAAGGA GTTGTAAGGC GTACAAGCCG GAGTTTGCAA 
GCACGTCCTG CTTCTATGCC AGTGGACAAA TTCCTTCTTG TAGGACTGCC GTGCACAGAA 
GATGAAACTG CTCAAGATGC CAAAGGAAAA GACAGTGCAA CTGCCAACAG TGATGGCGAT 
AAAACTGAGA AGGAAACAAA CTTGAAGGAA TCTCTTTTCA CGTGTTCTGA GAAAACTGCA 
GATCATTCAA ACTTGGCATC TCCCTTGAAA AAGGAGAGCG CCCAGAAGGA AACTACAGCT 
GATTTTTCTC TTGTACCCCA GGAGCAGCCC GAGCATTCGG TTGCTGTAAG TGATGTAACC 
GAAACAAACC GCCCCATAAG CAATCAGGAA AGTCTCCTTC CTGCTGAAAC GGTGCCTCTG 
ATTCCGGACT CTGCCTTTGA GCGCATCACA GAAAAAATAC AGGGCAAGAG CTGTGCAGGC 
AGTAGAAGGG CAGATGACAC AAACAGGTGT GAGGAGAAAA TAACTCCTGT CAGGACTTGC 
CTTCCTGAAA AAGTAGAGGA GGTTGAAAAT CTCAAATACG ACATCATCCA CAGAAGCACT 
GTGGCGTGTC TTTCTGTGAC AGATAGTTCT CCTGCCGTCT ACTCTGATGC TCCTGGTTTA 
TGCTCCTCTT CAACCATGCA AGACAGCGAC ATCTCCCGCC TCATAGACGA AGTTTCTTTG 
GTGACTGAGA GCGAGATGGA CAGCGCTGTC TCAGCTCTGA TCGGCCAGCT GGATGTCACC 
GATGACCAAA CCAGCCTCAC CGCACCGATG CTCCCTGGCG CTGGCAGCCG GGCCTTCAGT 
GCTGTGGCCA CACGCACACT GACTGCAGAG CTCAGCACTC CCTGTAAGAA GGACTTCATT 
GCCACACAGT CCAGCAAGTC CCCGTTATTT TCAACTCCAG ACTCTGCCGT GTTCCCCAGC 
CCCGAGACTG CGGAGTATTC TGTGTACACA ATTATCCACG AGGCAACTCT TACACCCGGT 
TCTGAATTTA AGACCCACAG TGGGTTAGTT TGCAAGAAGT CAAAGAGTGC AGAAAATAAC 
TTGCCTAGCT CTTTTTGTTC TTCACCTTTC TCTTTGCTGA ATGCAAATCC CCAAAGAAAA 
TGTGAAACAA GCTGGGAAGC CCCCGGCTCT GCGGGTTCTT TTGCTTCCAG TAGCCTCATC 
TTTGAGGAGA TGCTTGTGGA CCCTCCCATG GGCGAGAATT CGGAAAGCAG CAGTCTTGTA 
GAGTTAGGTG GGGATTCCCA AGACCTCTTG GTAACTGCGT GCGCGTACAA AAGGGAAGAT 
GTGAGCCAAT TGGCTTCCCC TTTAAAACTG AGGCAGCAGC AGGACAGCAG GTACTGTGGT 
GAGAGGACCT TTGGAAACTG TGCAAGTGCT GGGCTCTGTG CTGCTGCCCT GGACTGCTCG 
GATAACTTTA GGACTGCGGG AAGCAAGCTC CCTTTTCCAG CCTGTGAAAA TGTTGGTTTT 
CTACATCATC GTCACACAGA TGAGCAGAAA GATTTGATGT GTACCTCCAG GAGGTCCCAA 
CTCGATGTGC ACTCACCAGT GCTCAGAAGC GCGTTGGCTT TCCCACCCTC CCCAGCCATC 
AAACAGACCA GCCCTTGCAA GTCCAAGAGC CTTGGTGACT TGACATCGGA AGATATTTCC 
TGCAATTTTG AAAGTAAGTA TCAGTACATA AGTAGGAGCT TTGTCACATC TGGCATGAGA 
GACAAGAAGC TCGCTGCCAT GAAGACGATG AGACCGCACT CTACGGATGC TTTGACGGAA 
CAGCTGAGAA AGCTGCTGTC CCTGGACCAG GACGACAGCC AGCAGATGCT GTATTCCAGG 
CGGCTCGACG AGGACTGCCC AAAGGCACTG GTCAGAAAGC TGTCGTCCAG GAGCCAGAGC 
AGAGTGCGGA ACATCGCCAG CCGTGCCAAG GAGAGGCAGG AGGCCAGCAA GCAAAAATCT 
TCCACCGCAG GCAGCATAGC GGGGGTCGTC CTTCGGAACA AGCCTTCGGC GTCCCCTCAC 
GTCGTCAACA GGCACTCGAC GGGCTCCTAC ATAGCCAGGT ACCTGGGCAG CTTCCCTGAG 
GAGGCAGCAG AGGGCCGGGG CCGACCTGAG GGCACGTGTG CTGCCTGGCA CCGCGGCTGT 
GCTGAGCGGC TGTGTGCGCA CAGCCCCCTG CTGCAGCTGG AGCCCGACGG CGACGAGAAG 
CCTGAAGTCT ATTTCCTGCT GAGGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_015291823.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147309.1 Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X9, mRNA.

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CloneID	OGa12866
Clone ID Related Accession (Same CDS sequence)	XM_025153773.1 , XM_015291823.1
Accession Version	XM_015291823.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGTAGA 
CTGACTCGTC GAAGGAAAAC TGTGAAGCTG TGCCGAGCTC TGTCTGACCT AGTGGTGTAC 
ACGAACTCTG TGGCAGCCCA GGATATAGTA GATGATGGAT CAACAGGGAA TGTGCTGTCA 
TTCAGTGAAA CAAGAGCCCA CCAAGCAGTG CAGCAGAAGG CTGAACAGTT CATGCTTTAC 
AACCAGAAAC AGCTTACAAG GGTTTATCCA TCAGCGTATC GGATTGACTC CAGCAATTTC 
AATCCTTTAC CTTACTGGAA TGTTGGTTGC CAGCTAGTGG CACTAAACTA CCAGTCTGAG 
GGACGTATGA TGCAATTAAA CGAAGCAAAA TTTAGGGTAA ATGGCAACTG TGGTTACGTC 
CTCAAACCTC AGCAGATGTG CAAAGGCTCA TTCAACCCCT ATTCTTCAGA TCCACTTCCT 
GCTAGTCCTA AGAAGCAGCT CATCCTGAAA ATCATCAGTG GACAGCAGCT GCCTAAGCCT 
CCTGACTCAA TGCTAGGAGA CAGAGGAGAG ATAATAGATC CCTTTGTCGA GGTGGAAATT 
ATTGGGTTAC CTGTTGACTG CTGTAAAGAT CAGACCAGGG TGGTTGATGA CAATGGGTTT 
AATCCTGTTT GGGAGGAAAC GCTCACTTTT ACCATCCATA TGCCTGAAAT AGCTTTGGTT 
CGATTTCTTG TTTGGGATCA TGACCCCATT GGGCGAGACT TCGTTGGACA AAGAACGCTG 
GCCTTTAGCA GTCTTGTACC TGGTTATCGA CATGTGTATT TAGAAGGGCT GACAGAAGCT 
TCCATATTTG TTCACATAAC CATTAATGAA ATCTATGGGA AGAGTAAGCA ACTGATCGGA 
CTCCGAGGGC TGTTTAACAA GAACCCAAAG CACAGTTCTG CTGAAAGCAG CGGTCATTAT 
GTACGGAAGC GTTCTATTGG TGACCGAATT CTCCGGCGCA CGGCCAGTGC TCCAGCAAAA 
GGTAGAAAGA AAAGCAAGAT GGGTTTTCTG GAAGCTTCTG AAATTAAAGA CAGTGCATCC 
GAACCAGCAG ACCTGAAGGA CAAAGAAGGA GTTGTAAGGC GTACAAGCCG GAGTTTGCAA 
GCACGTCCTG CTTCTATGCC AGTGGACAAA TTCCTTCTTG TAGGACTGCC GTGCACAGAA 
GATGAAACTG CTCAAGATGC CAAAGGAAAA GACAGTGCAA CTGCCAACAG TGATGGCGAT 
AAAACTGAGA AGGAAACAAA CTTGAAGGAA TCTCTTTTCA CGTGTTCTGA GAAAACTGCA 
GATCATTCAA ACTTGGCATC TCCCTTGAAA AAGGAGAGCG CCCAGAAGGA AACTACAGCT 
GATTTTTCTC TTGTACCCCA GGAGCAGCCC GAGCATTCGG TTGCTGTAAG TGATGTAACC 
GAAACAAACC GCCCCATAAG CAATCAGGAA AGTCTCCTTC CTGCTGAAAC GGTGCCTCTG 
ATTCCGGACT CTGCCTTTGA GCGCATCACA GAAAAAATAC AGGGCAAGAG CTGTGCAGGC 
AGTAGAAGGG CAGATGACAC AAACAGGTGT GAGGAGAAAA TAACTCCTGT CAGGACTTGC 
CTTCCTGAAA AAGTAGAGGA GGTTGAAAAT CTCAAATACG ACATCATCCA CAGAAGCACT 
GTGGCGTGTC TTTCTGTGAC AGATAGTTCT CCTGCCGTCT ACTCTGATGC TCCTGGTTTA 
TGCTCCTCTT CAACCATGCA AGACAGCGAC ATCTCCCGCC TCATAGACGA AGTTTCTTTG 
GTGACTGAGA GCGAGATGGA CAGCGCTGTC TCAGCTCTGA TCGGCCAGCT GGATGTCACC 
GATGACCAAA CCAGCCTCAC CGCACCGATG CTCCCTGGCG CTGGCAGCCG GGCCTTCAGT 
GCTGTGGCCA CACGCACACT GACTGCAGAG CTCAGCACTC CCTGTAAGAA GGACTTCATT 
GCCACACAGT CCAGCAAGTC CCCGTTATTT TCAACTCCAG ACTCTGCCGT GTTCCCCAGC 
CCCGAGACTG CGGAGTATTC TGTGTACACA ATTATCCACG AGGCAACTCT TACACCCGGT 
TCTGAATTTA AGACCCACAG TGGGTTAGTT TGCAAGAAGT CAAAGAGTGC AGAAAATAAC 
TTGCCTAGCT CTTTTTGTTC TTCACCTTTC TCTTTGCTGA ATGCAAATCC CCAAAGAAAA 
TGTGAAACAA GCTGGGAAGC CCCCGGCTCT GCGGGTTCTT TTGCTTCCAG TAGCCTCATC 
TTTGAGGAGA TGCTTGTGGA CCCTCCCATG GGCGAGAATT CGGAAAGCAG CAGTCTTGTA 
GAGTTAGGTG GGGATTCCCA AGACCTCTTG GTAACTGCGT GCGCGTACAA AAGGGAAGAT 
GTGAGCCAAT TGGCTTCCCC TTTAAAACTG AGGCAGCAGC AGGACAGCAG GTACTGTGGT 
GAGAGGACCT TTGGAAACTG TGCAAGTGCT GGGCTCTGTG CTGCTGCCCT GGACTGCTCG 
GATAACTTTA GGACTGCGGG AAGCAAGCTC CCTTTTCCAG CCTGTGAAAA TGTTGGTTTT 
CTACATCATC GTCACACAGA TGAGCAGAAA GATTTGATGT GTACCTCCAG GAGGTCCCAA 
CTCGATGTGC ACTCACCAGT GCTCAGAAGC GCGTTGGCTT TCCCACCCTC CCCAGCCATC 
AAACAGACCA GCCCTTGCAA GTCCAAGAGC CTTGGTGACT TGACATCGGA AGATATTTCC 
TGCAATTTTG AAAGTAAGTA TCAGTACATA AGTAGGAGCT TTGTCACATC TGGCATGAGA 
GACAAGAAGC TCGCTGCCAT GAAGACGATG AGACCGCACT CTACGGATGC TTTGACGGAA 
CAGCTGAGAA AGCTGCTGTC CCTGGACCAG GACGACAGCC AGCAGATGCT GTATTCCAGG 
CGGCTCGACG AGGACTGCCC AAAGGCACTG GTCAGAAAGC TGTCGTCCAG GAGCCAGAGC 
AGAGTGCGGA ACATCGCCAG CCGTGCCAAG GAGAGGCAGG AGGCCAGCAA GCAAAAATCT 
TCCACCGCAG GCAGCATAGC GGGGGTCGTC CTTCGGAACA AGCCTTCGGC GTCCCCTCAC 
GTCGTCAACA GGCACTCGAC GGGCTCCTAC ATAGCCAGGT ACCTGGGCAG CTTCCCTGAG 
GAGGCAGCAG AGGGCCGGGG CCGACCTGAG GGCACGTGTG CTGCCTGGCA CCGCGGCTGT 
GCTGAGCGGC TGTGTGCGCA CAGCCCCCTG CTGCAGCTGG AGCCCGACGG CGACGAGAAG 
CCTGAAGTCT ATTTCCTGCT GAGGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147309.1
CDS	1381..6450
Translation	MSYWSLDKKSCLQYCRHFLADNGVFHVERCMSVMQSGTQMVKLKSGTKGLVRLFYLDEHRTCIRWRPSRKSEKAKIVIDSIYKVTEGRQSEIFHRHAEGSFDPSCCFTIYHGNHLESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDHWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLNFEEFSVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTPEYCLDIIQKFEVSEENKEQNVLGIEGFTNFMRSPACDVFNPLHCEVHQDMDQPLCNYFIASSHNTYLTGDQLLSQSRVEMYARVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKIFFRDVAETINKYAFIKNEFPVILSIENHCSIQQQKKIAQYLKEILGDKLDLSSVVTGDSRQLPSPQNLKGKILVKGKKLPYSLGADAEEGEVSDEDSADEIEDDCKLKSCYSNGATEHQVESFIRTKLESLLKESQIRDKEDPDSFTVRALLRATHEGLSVNLKQNLDTKEGGKKSHSRSLMGNFGKHKKTGKATSKCSTSDDDENQQNSSGKEAGQLHSRLTRRRKTVKLCRALSDLVVYTNSVAAQDIVDDGSTGNVLSFSETRAHQAVQQKAEQFMLYNQKQLTRVYPSAYRIDSSNFNPLPYWNVGCQLVALNYQSEGRMMQLNEAKFRVNGNCGYVLKPQQMCKGSFNPYSSDPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTIHMPEIALVRFLVWDHDPIGRDFVGQRTLAFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKSKQLIGLRGLFNKNPKHSSAESSGHYVRKRSIGDRILRRTASAPAKGRKKSKMGFLEASEIKDSASEPADLKDKEGVVRRTSRSLQARPASMPVDKFLLVGLPCTEDETAQDAKGKDSATANSDGDKTEKETNLKESLFTCSEKTADHSNLASPLKKESAQKETTADFSLVPQEQPEHSVAVSDVTETNRPISNQESLLPAETVPLIPDSAFERITEKIQGKSCAGSRRADDTNRCEEKITPVRTCLPEKVEEVENLKYDIIHRSTVACLSVTDSSPAVYSDAPGLCSSSTMQDSDISRLIDEVSLVTESEMDSAVSALIGQLDVTDDQTSLTAPMLPGAGSRAFSAVATRTLTAELSTPCKKDFIATQSSKSPLFSTPDSAVFPSPETAEYSVYTIIHEATLTPGSEFKTHSGLVCKKSKSAENNLPSSFCSSPFSLLNANPQRKCETSWEAPGSAGSFASSSLIFEEMLVDPPMGENSESSSLVELGGDSQDLLVTACAYKREDVSQLASPLKLRQQQDSRYCGERTFGNCASAGLCAAALDCSDNFRTAGSKLPFPACENVGFLHHRHTDEQKDLMCTSRRSQLDVHSPVLRSALAFPPSPAIKQTSPCKSKSLGDLTSEDISCNFESKYQYISRSFVTSGMRDKKLAAMKTMRPHSTDALTEQLRKLLSLDQDDSQQMLYSRRLDEDCPKALVRKLSSRSQSRVRNIASRAKERQEASKQKSSTAGSIAGVVLRNKPSASPHVVNRHSTGSYIARYLGSFPEEAAEGRGRPEGTCAAWHRGCAERLCAHSPLLQLEPDGDEKPEVYFLLRL

Target ORF information:

RefSeq Version	XM_015291823.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291823.1

ORF Insert Sequence:

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGTAGA 
CTGACTCGTC GAAGGAAAAC TGTGAAGCTG TGCCGAGCTC TGTCTGACCT AGTGGTGTAC 
ACGAACTCTG TGGCAGCCCA GGATATAGTA GATGATGGAT CAACAGGGAA TGTGCTGTCA 
TTCAGTGAAA CAAGAGCCCA CCAAGCAGTG CAGCAGAAGG CTGAACAGTT CATGCTTTAC 
AACCAGAAAC AGCTTACAAG GGTTTATCCA TCAGCGTATC GGATTGACTC CAGCAATTTC 
AATCCTTTAC CTTACTGGAA TGTTGGTTGC CAGCTAGTGG CACTAAACTA CCAGTCTGAG 
GGACGTATGA TGCAATTAAA CGAAGCAAAA TTTAGGGTAA ATGGCAACTG TGGTTACGTC 
CTCAAACCTC AGCAGATGTG CAAAGGCTCA TTCAACCCCT ATTCTTCAGA TCCACTTCCT 
GCTAGTCCTA AGAAGCAGCT CATCCTGAAA ATCATCAGTG GACAGCAGCT GCCTAAGCCT 
CCTGACTCAA TGCTAGGAGA CAGAGGAGAG ATAATAGATC CCTTTGTCGA GGTGGAAATT 
ATTGGGTTAC CTGTTGACTG CTGTAAAGAT CAGACCAGGG TGGTTGATGA CAATGGGTTT 
AATCCTGTTT GGGAGGAAAC GCTCACTTTT ACCATCCATA TGCCTGAAAT AGCTTTGGTT 
CGATTTCTTG TTTGGGATCA TGACCCCATT GGGCGAGACT TCGTTGGACA AAGAACGCTG 
GCCTTTAGCA GTCTTGTACC TGGTTATCGA CATGTGTATT TAGAAGGGCT GACAGAAGCT 
TCCATATTTG TTCACATAAC CATTAATGAA ATCTATGGGA AGAGTAAGCA ACTGATCGGA 
CTCCGAGGGC TGTTTAACAA GAACCCAAAG CACAGTTCTG CTGAAAGCAG CGGTCATTAT 
GTACGGAAGC GTTCTATTGG TGACCGAATT CTCCGGCGCA CGGCCAGTGC TCCAGCAAAA 
GGTAGAAAGA AAAGCAAGAT GGGTTTTCTG GAAGCTTCTG AAATTAAAGA CAGTGCATCC 
GAACCAGCAG ACCTGAAGGA CAAAGAAGGA GTTGTAAGGC GTACAAGCCG GAGTTTGCAA 
GCACGTCCTG CTTCTATGCC AGTGGACAAA TTCCTTCTTG TAGGACTGCC GTGCACAGAA 
GATGAAACTG CTCAAGATGC CAAAGGAAAA GACAGTGCAA CTGCCAACAG TGATGGCGAT 
AAAACTGAGA AGGAAACAAA CTTGAAGGAA TCTCTTTTCA CGTGTTCTGA GAAAACTGCA 
GATCATTCAA ACTTGGCATC TCCCTTGAAA AAGGAGAGCG CCCAGAAGGA AACTACAGCT 
GATTTTTCTC TTGTACCCCA GGAGCAGCCC GAGCATTCGG TTGCTGTAAG TGATGTAACC 
GAAACAAACC GCCCCATAAG CAATCAGGAA AGTCTCCTTC CTGCTGAAAC GGTGCCTCTG 
ATTCCGGACT CTGCCTTTGA GCGCATCACA GAAAAAATAC AGGGCAAGAG CTGTGCAGGC 
AGTAGAAGGG CAGATGACAC AAACAGGTGT GAGGAGAAAA TAACTCCTGT CAGGACTTGC 
CTTCCTGAAA AAGTAGAGGA GGTTGAAAAT CTCAAATACG ACATCATCCA CAGAAGCACT 
GTGGCGTGTC TTTCTGTGAC AGATAGTTCT CCTGCCGTCT ACTCTGATGC TCCTGGTTTA 
TGCTCCTCTT CAACCATGCA AGACAGCGAC ATCTCCCGCC TCATAGACGA AGTTTCTTTG 
GTGACTGAGA GCGAGATGGA CAGCGCTGTC TCAGCTCTGA TCGGCCAGCT GGATGTCACC 
GATGACCAAA CCAGCCTCAC CGCACCGATG CTCCCTGGCG CTGGCAGCCG GGCCTTCAGT 
GCTGTGGCCA CACGCACACT GACTGCAGAG CTCAGCACTC CCTGTAAGAA GGACTTCATT 
GCCACACAGT CCAGCAAGTC CCCGTTATTT TCAACTCCAG ACTCTGCCGT GTTCCCCAGC 
CCCGAGACTG CGGAGTATTC TGTGTACACA ATTATCCACG AGGCAACTCT TACACCCGGT 
TCTGAATTTA AGACCCACAG TGGGTTAGTT TGCAAGAAGT CAAAGAGTGC AGAAAATAAC 
TTGCCTAGCT CTTTTTGTTC TTCACCTTTC TCTTTGCTGA ATGCAAATCC CCAAAGAAAA 
TGTGAAACAA GCTGGGAAGC CCCCGGCTCT GCGGGTTCTT TTGCTTCCAG TAGCCTCATC 
TTTGAGGAGA TGCTTGTGGA CCCTCCCATG GGCGAGAATT CGGAAAGCAG CAGTCTTGTA 
GAGTTAGGTG GGGATTCCCA AGACCTCTTG GTAACTGCGT GCGCGTACAA AAGGGAAGAT 
GTGAGCCAAT TGGCTTCCCC TTTAAAACTG AGGCAGCAGC AGGACAGCAG GTACTGTGGT 
GAGAGGACCT TTGGAAACTG TGCAAGTGCT GGGCTCTGTG CTGCTGCCCT GGACTGCTCG 
GATAACTTTA GGACTGCGGG AAGCAAGCTC CCTTTTCCAG CCTGTGAAAA TGTTGGTTTT 
CTACATCATC GTCACACAGA TGAGCAGAAA GATTTGATGT GTACCTCCAG GAGGTCCCAA 
CTCGATGTGC ACTCACCAGT GCTCAGAAGC GCGTTGGCTT TCCCACCCTC CCCAGCCATC 
AAACAGACCA GCCCTTGCAA GTCCAAGAGC CTTGGTGACT TGACATCGGA AGATATTTCC 
TGCAATTTTG AAAGTAAGTA TCAGTACATA AGTAGGAGCT TTGTCACATC TGGCATGAGA 
GACAAGAAGC TCGCTGCCAT GAAGACGATG AGACCGCACT CTACGGATGC TTTGACGGAA 
CAGCTGAGAA AGCTGCTGTC CCTGGACCAG GACGACAGCC AGCAGATGCT GTATTCCAGG 
CGGCTCGACG AGGACTGCCC AAAGGCACTG GTCAGAAAGC TGTCGTCCAG GAGCCAGAGC 
AGAGTGCGGA ACATCGCCAG CCGTGCCAAG GAGAGGCAGG AGGCCAGCAA GCAAAAATCT 
TCCACCGCAG GCAGCATAGC GGGGGTCGTC CTTCGGAACA AGCCTTCGGC GTCCCCTCAC 
GTCGTCAACA GGCACTCGAC GGGCTCCTAC ATAGCCAGGT ACCTGGGCAG CTTCCCTGAG 
GAGGCAGCAG AGGGCCGGGG CCGACCTGAG GGCACGTGTG CTGCCTGGCA CCGCGGCTGT 
GCTGAGCGGC TGTGTGCGCA CAGCCCCCTG CTGCAGCTGG AGCCCGACGG CGACGAGAAG 
CCTGAAGTCT ATTTCCTGCT GAGGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_025153774.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025009542.1 Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X12, mRNA.

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CloneID	OGa12867
Clone ID Related Accession (Same CDS sequence)	XM_025153774.1 , XM_004943562.2
Accession Version	XM_025153774.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGACTG 
ACTCGTCGAA GGAAAACTGT GAAGCTGTGC CGAGCTCTGT CTGACCTAGT GGTGTACACG 
AACTCTGTGG CAGCCCAGGA TATAGTAGAT GATGGATCAA CAGGGAATGT GCTGTCATTC 
AGTGAAACAA GAGCCCACCA AGCAGTGCAG CAGAAGGCTG AACAGTTCAT GCTTTACAAC 
CAGAAACAGC TTACAAGGGT TTATCCATCA GCGTATCGGA TTGACTCCAG CAATTTCAAT 
CCTTTACCTT ACTGGAATGT TGGTTGCCAG CTAGTGGCAC TAAACTACCA GTCTGAGGGA 
CGTATGATGC AATTAAACGA AGCAAAATTT AGGGTAAATG GCAACTGTGG TTACGTCCTC 
AAACCTCAGC AGATGTGCAA AGGCTCATTC AACCCCTATT CTTCAGATCC ACTTCCTGCT 
AGTCCTAAGA AGCAGCTCAT CCTGAAAATC ATCAGTGGAC AGCAGCTGCC TAAGCCTCCT 
GACTCAATGC TAGGAGACAG AGGAGAGATA ATAGATCCCT TTGTCGAGGT GGAAATTATT 
GGGTTACCTG TTGACTGCTG TAAAGATCAG ACCAGGGTGG TTGATGACAA TGGGTTTAAT 
CCTGTTTGGG AGGAAACGCT CACTTTTACC ATCCATATGC CTGAAATAGC TTTGGTTCGA 
TTTCTTGTTT GGGATCATGA CCCCATTGGG CGAGACTTCG TTGGACAAAG AACGCTGGCC 
TTTAGCAGTC TTGTACCTGG TTATCGACAT GTGTATTTAG AAGGGCTGAC AGAAGCTTCC 
ATATTTGTTC ACATAACCAT TAATGAAATC TATGGGAAGA GTAAGCAACT GATCGGACTC 
CGAGGGCTGT TTAACAAGAA CCCAAAGCAC AGTTCTGCTG AAAGCAGCGG TCATTATGTA 
CGGAAGCGTT CTATTGGTGA CCGAATTCTC CGGCGCACGG CCAGTGCTCC AGCAAAAGGT 
AGAAAGAAAA GCAAGATGGG TTTTCTGGAA GCTTCTGAAA TTAAAGACAG TGCATCCGAA 
CCAGCAGACC TGAAGGACAA AGAAGGAGTT GTAAGGCGTA CAAGCCGGAG TTTGCAAGCA 
CGTCCTGCTT CTATGCCAGT GGACAAATTC CTTCTTGTAG GACTGCCGTG CACAGAAGAT 
GAAACTGCTC AAGATGCCAA AGGAAAAGAC AGTGCAACTG CCAACAGTGA TGGCGATAAA 
ACTGAGAAGG AAACAAACTT GAAGGAATCT CTTTTCACGT GTTCTGAGAA AACTGCAGAT 
CATTCAAACT TGGCATCTCC CTTGAAAAAG GAGAGCGCCC AGAAGGAAAC TACAGCTGAT 
TTTTCTCTTG TACCCCAGGA GCAGCCCGAG CATTCGGTTG CTGTAAGTGA TGTAACCGAA 
ACAAACCGCC CCATAAGCAA TCAGGAAAGT CTCCTTCCTG CTGAAACGGT GCCTCTGATT 
CCGGACTCTG CCTTTGAGCG CATCACAGAA AAAATACAGG GCAAGAGCTG TGCAGGCAGT 
AGAAGGGCAG ATGACACAAA CAGGTGTGAG GAGAAAATAA CTCCTGTCAG GACTTGCCTT 
CCTGAAAAAG TAGAGGAGGT TGAAAATCTC AAATACGACA TCATCCACAG AAGCACTGTG 
GCGTGTCTTT CTGTGACAGA TAGTTCTCCT GCCGTCTACT CTGATGCTCC TGGTTTATGC 
TCCTCTTCAA CCATGCAAGA CAGCGACATC TCCCGCCTCA TAGACGAAGT TTCTTTGGTG 
ACTGAGAGCG AGATGGACAG CGCTGTCTCA GCTCTGATCG GCCAGCTGGA TGTCACCGAT 
GACCAAACCA GCCTCACCGC ACCGATGCTC CCTGGCGCTG GCAGCCGGGC CTTCAGTGCT 
GTGGCCACAC GCACACTGAC TGCAGAGCTC AGCACTCCCT GTAAGAAGGA CTTCATTGCC 
ACACAGTCCA GCAAGTCCCC GTTATTTTCA ACTCCAGACT CTGCCGTGTT CCCCAGCCCC 
GAGACTGCGG AGTATTCTGT GTACACAATT ATCCACGAGG CAACTCTTAC ACCCGGTTCT 
GAATTTAAGA CCCACAGTGG GTTAGTTTGC AAGAAGTCAA AGAGTGCAGA AAATAACTTG 
CCTAGCTCTT TTTGTTCTTC ACCTTTCTCT TTGCTGAATG CAAATCCCCA AAGAAAATGT 
GAAACAAGCT GGGAAGCCCC CGGCTCTGCG GGTTCTTTTG CTTCCAGTAG CCTCATCTTT 
GAGGAGATGC TTGTGGACCC TCCCATGGGC GAGAATTCGG AAAGCAGCAG TCTTGTAGAG 
TTAGGTGGGG ATTCCCAAGA CCTCTTGGTA ACTGCGTGCG CGTACAAAAG GGAAGATGTG 
AGCCAATTGG CTTCCCCTTT AAAACTGAGG CAGCAGCAGG ACAGCAGGTA CTGTGGTGAG 
AGGACCTTTG GAAACTGTGC AAGTGCTGGG CTCTGTGCTG CTGCCCTGGA CTGCTCGGAT 
AACTTTAGGA CTGCGGGAAG CAAGCTCCCT TTTCCAGCCT GTGAAAATGT TGGTTTTCTA 
CATCATCGTC ACACAGATGA GCAGAAAGAT TTGATGTGTA CCTCCAGGAG GTCCCAACTC 
GATGTGCACT CACCAGTGCT CAGAAGCGCG TTGGCTTTCC CACCCTCCCC AGCCATCAAA 
CAGACCAGCC CTTGCAAGTC CAAGAGCCTT GGTGACTTGA CATCGGAAGA TATTTCCTGC 
AATTTTGAAA GTAAGTATCA GTACATAAGT AGGAGCTTTG TCACATCTGG CATGAGAGAC 
AAGAAGCTCG CTGCCATGAA GACGATGAGA CCGCACTCTA CGGATGCTTT GACGGAACAG 
CTGAGAAAGC TGCTGTCCCT GGACCAGGAC GACAGCCAGC AGATGCTGTA TTCCAGGCGG 
CTCGACGAGG ACTGCCCAAA GGCACTGGTC AGAAAGCTGT CGTCCAGGAG CCAGAGCAGA 
GTGCGGAACA TCGCCAGCCG TGCCAAGGAG AGGCAGGAGG CCAGCAAGCA AAAATCTTCC 
ACCGCAGGCA GCATAGCGGG GGTCGTCCTT CGGAACAAGC CTTCGGCGTC CCCTCACGTC 
GTCAACAGGC ACTCGACGGG CTCCTACATA GCCAGGTACC TGGGCAGCTT CCCTGAGGAG 
GCAGCAGAGG GCCGGGGCCG ACCTGAGGGC ACGTGTGCTG CCTGGCACCG CGGCTGTGCT 
GAGCGGCTGT GTGCGCACAG CCCCCTGCTG CAGCTGGAGC CCGACGGCGA CGAGAAGCCT 
GAAGTCTATT TCCTGCTGAG GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025009542.1
CDS	337..5403
Translation	MSYWSLDKKSCLQYCRHFLADNGVFHVERCMSVMQSGTQMVKLKSGTKGLVRLFYLDEHRTCIRWRPSRKSEKAKIVIDSIYKVTEGRQSEIFHRHAEGSFDPSCCFTIYHGNHLESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDHWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLNFEEFSVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTPEYCLDIIQKFEVSEENKEQNVLGIEGFTNFMRSPACDVFNPLHCEVHQDMDQPLCNYFIASSHNTYLTGDQLLSQSRVEMYARVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKIFFRDVAETINKYAFIKNEFPVILSIENHCSIQQQKKIAQYLKEILGDKLDLSSVVTGDSRQLPSPQNLKGKILVKGKKLPYSLGADAEEGEVSDEDSADEIEDDCKLKSCYSNGATEHQVESFIRTKLESLLKESQIRDKEDPDSFTVRALLRATHEGLSVNLKQNLDTKEGGKKSHSRSLMGNFGKHKKTGKATSKCSTSDDDENQQNSSGKEAGQLHRLTRRRKTVKLCRALSDLVVYTNSVAAQDIVDDGSTGNVLSFSETRAHQAVQQKAEQFMLYNQKQLTRVYPSAYRIDSSNFNPLPYWNVGCQLVALNYQSEGRMMQLNEAKFRVNGNCGYVLKPQQMCKGSFNPYSSDPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTIHMPEIALVRFLVWDHDPIGRDFVGQRTLAFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKSKQLIGLRGLFNKNPKHSSAESSGHYVRKRSIGDRILRRTASAPAKGRKKSKMGFLEASEIKDSASEPADLKDKEGVVRRTSRSLQARPASMPVDKFLLVGLPCTEDETAQDAKGKDSATANSDGDKTEKETNLKESLFTCSEKTADHSNLASPLKKESAQKETTADFSLVPQEQPEHSVAVSDVTETNRPISNQESLLPAETVPLIPDSAFERITEKIQGKSCAGSRRADDTNRCEEKITPVRTCLPEKVEEVENLKYDIIHRSTVACLSVTDSSPAVYSDAPGLCSSSTMQDSDISRLIDEVSLVTESEMDSAVSALIGQLDVTDDQTSLTAPMLPGAGSRAFSAVATRTLTAELSTPCKKDFIATQSSKSPLFSTPDSAVFPSPETAEYSVYTIIHEATLTPGSEFKTHSGLVCKKSKSAENNLPSSFCSSPFSLLNANPQRKCETSWEAPGSAGSFASSSLIFEEMLVDPPMGENSESSSLVELGGDSQDLLVTACAYKREDVSQLASPLKLRQQQDSRYCGERTFGNCASAGLCAAALDCSDNFRTAGSKLPFPACENVGFLHHRHTDEQKDLMCTSRRSQLDVHSPVLRSALAFPPSPAIKQTSPCKSKSLGDLTSEDISCNFESKYQYISRSFVTSGMRDKKLAAMKTMRPHSTDALTEQLRKLLSLDQDDSQQMLYSRRLDEDCPKALVRKLSSRSQSRVRNIASRAKERQEASKQKSSTAGSIAGVVLRNKPSASPHVVNRHSTGSYIARYLGSFPEEAAEGRGRPEGTCAAWHRGCAERLCAHSPLLQLEPDGDEKPEVYFLLRL

Target ORF information:

RefSeq Version	XM_025153774.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025153774.1

ORF Insert Sequence:

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGACTG 
ACTCGTCGAA GGAAAACTGT GAAGCTGTGC CGAGCTCTGT CTGACCTAGT GGTGTACACG 
AACTCTGTGG CAGCCCAGGA TATAGTAGAT GATGGATCAA CAGGGAATGT GCTGTCATTC 
AGTGAAACAA GAGCCCACCA AGCAGTGCAG CAGAAGGCTG AACAGTTCAT GCTTTACAAC 
CAGAAACAGC TTACAAGGGT TTATCCATCA GCGTATCGGA TTGACTCCAG CAATTTCAAT 
CCTTTACCTT ACTGGAATGT TGGTTGCCAG CTAGTGGCAC TAAACTACCA GTCTGAGGGA 
CGTATGATGC AATTAAACGA AGCAAAATTT AGGGTAAATG GCAACTGTGG TTACGTCCTC 
AAACCTCAGC AGATGTGCAA AGGCTCATTC AACCCCTATT CTTCAGATCC ACTTCCTGCT 
AGTCCTAAGA AGCAGCTCAT CCTGAAAATC ATCAGTGGAC AGCAGCTGCC TAAGCCTCCT 
GACTCAATGC TAGGAGACAG AGGAGAGATA ATAGATCCCT TTGTCGAGGT GGAAATTATT 
GGGTTACCTG TTGACTGCTG TAAAGATCAG ACCAGGGTGG TTGATGACAA TGGGTTTAAT 
CCTGTTTGGG AGGAAACGCT CACTTTTACC ATCCATATGC CTGAAATAGC TTTGGTTCGA 
TTTCTTGTTT GGGATCATGA CCCCATTGGG CGAGACTTCG TTGGACAAAG AACGCTGGCC 
TTTAGCAGTC TTGTACCTGG TTATCGACAT GTGTATTTAG AAGGGCTGAC AGAAGCTTCC 
ATATTTGTTC ACATAACCAT TAATGAAATC TATGGGAAGA GTAAGCAACT GATCGGACTC 
CGAGGGCTGT TTAACAAGAA CCCAAAGCAC AGTTCTGCTG AAAGCAGCGG TCATTATGTA 
CGGAAGCGTT CTATTGGTGA CCGAATTCTC CGGCGCACGG CCAGTGCTCC AGCAAAAGGT 
AGAAAGAAAA GCAAGATGGG TTTTCTGGAA GCTTCTGAAA TTAAAGACAG TGCATCCGAA 
CCAGCAGACC TGAAGGACAA AGAAGGAGTT GTAAGGCGTA CAAGCCGGAG TTTGCAAGCA 
CGTCCTGCTT CTATGCCAGT GGACAAATTC CTTCTTGTAG GACTGCCGTG CACAGAAGAT 
GAAACTGCTC AAGATGCCAA AGGAAAAGAC AGTGCAACTG CCAACAGTGA TGGCGATAAA 
ACTGAGAAGG AAACAAACTT GAAGGAATCT CTTTTCACGT GTTCTGAGAA AACTGCAGAT 
CATTCAAACT TGGCATCTCC CTTGAAAAAG GAGAGCGCCC AGAAGGAAAC TACAGCTGAT 
TTTTCTCTTG TACCCCAGGA GCAGCCCGAG CATTCGGTTG CTGTAAGTGA TGTAACCGAA 
ACAAACCGCC CCATAAGCAA TCAGGAAAGT CTCCTTCCTG CTGAAACGGT GCCTCTGATT 
CCGGACTCTG CCTTTGAGCG CATCACAGAA AAAATACAGG GCAAGAGCTG TGCAGGCAGT 
AGAAGGGCAG ATGACACAAA CAGGTGTGAG GAGAAAATAA CTCCTGTCAG GACTTGCCTT 
CCTGAAAAAG TAGAGGAGGT TGAAAATCTC AAATACGACA TCATCCACAG AAGCACTGTG 
GCGTGTCTTT CTGTGACAGA TAGTTCTCCT GCCGTCTACT CTGATGCTCC TGGTTTATGC 
TCCTCTTCAA CCATGCAAGA CAGCGACATC TCCCGCCTCA TAGACGAAGT TTCTTTGGTG 
ACTGAGAGCG AGATGGACAG CGCTGTCTCA GCTCTGATCG GCCAGCTGGA TGTCACCGAT 
GACCAAACCA GCCTCACCGC ACCGATGCTC CCTGGCGCTG GCAGCCGGGC CTTCAGTGCT 
GTGGCCACAC GCACACTGAC TGCAGAGCTC AGCACTCCCT GTAAGAAGGA CTTCATTGCC 
ACACAGTCCA GCAAGTCCCC GTTATTTTCA ACTCCAGACT CTGCCGTGTT CCCCAGCCCC 
GAGACTGCGG AGTATTCTGT GTACACAATT ATCCACGAGG CAACTCTTAC ACCCGGTTCT 
GAATTTAAGA CCCACAGTGG GTTAGTTTGC AAGAAGTCAA AGAGTGCAGA AAATAACTTG 
CCTAGCTCTT TTTGTTCTTC ACCTTTCTCT TTGCTGAATG CAAATCCCCA AAGAAAATGT 
GAAACAAGCT GGGAAGCCCC CGGCTCTGCG GGTTCTTTTG CTTCCAGTAG CCTCATCTTT 
GAGGAGATGC TTGTGGACCC TCCCATGGGC GAGAATTCGG AAAGCAGCAG TCTTGTAGAG 
TTAGGTGGGG ATTCCCAAGA CCTCTTGGTA ACTGCGTGCG CGTACAAAAG GGAAGATGTG 
AGCCAATTGG CTTCCCCTTT AAAACTGAGG CAGCAGCAGG ACAGCAGGTA CTGTGGTGAG 
AGGACCTTTG GAAACTGTGC AAGTGCTGGG CTCTGTGCTG CTGCCCTGGA CTGCTCGGAT 
AACTTTAGGA CTGCGGGAAG CAAGCTCCCT TTTCCAGCCT GTGAAAATGT TGGTTTTCTA 
CATCATCGTC ACACAGATGA GCAGAAAGAT TTGATGTGTA CCTCCAGGAG GTCCCAACTC 
GATGTGCACT CACCAGTGCT CAGAAGCGCG TTGGCTTTCC CACCCTCCCC AGCCATCAAA 
CAGACCAGCC CTTGCAAGTC CAAGAGCCTT GGTGACTTGA CATCGGAAGA TATTTCCTGC 
AATTTTGAAA GTAAGTATCA GTACATAAGT AGGAGCTTTG TCACATCTGG CATGAGAGAC 
AAGAAGCTCG CTGCCATGAA GACGATGAGA CCGCACTCTA CGGATGCTTT GACGGAACAG 
CTGAGAAAGC TGCTGTCCCT GGACCAGGAC GACAGCCAGC AGATGCTGTA TTCCAGGCGG 
CTCGACGAGG ACTGCCCAAA GGCACTGGTC AGAAAGCTGT CGTCCAGGAG CCAGAGCAGA 
GTGCGGAACA TCGCCAGCCG TGCCAAGGAG AGGCAGGAGG CCAGCAAGCA AAAATCTTCC 
ACCGCAGGCA GCATAGCGGG GGTCGTCCTT CGGAACAAGC CTTCGGCGTC CCCTCACGTC 
GTCAACAGGC ACTCGACGGG CTCCTACATA GCCAGGTACC TGGGCAGCTT CCCTGAGGAG 
GCAGCAGAGG GCCGGGGCCG ACCTGAGGGC ACGTGTGCTG CCTGGCACCG CGGCTGTGCT 
GAGCGGCTGT GTGCGCACAG CCCCCTGCTG CAGCTGGAGC CCGACGGCGA CGAGAAGCCT 
GAAGTCTATT TCCTGCTGAG GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_004943562.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004943619.1 Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X10, mRNA.

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CloneID	OGa12867
Clone ID Related Accession (Same CDS sequence)	XM_025153774.1 , XM_004943562.2
Accession Version	XM_004943562.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004943562.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGACTG 
ACTCGTCGAA GGAAAACTGT GAAGCTGTGC CGAGCTCTGT CTGACCTAGT GGTGTACACG 
AACTCTGTGG CAGCCCAGGA TATAGTAGAT GATGGATCAA CAGGGAATGT GCTGTCATTC 
AGTGAAACAA GAGCCCACCA AGCAGTGCAG CAGAAGGCTG AACAGTTCAT GCTTTACAAC 
CAGAAACAGC TTACAAGGGT TTATCCATCA GCGTATCGGA TTGACTCCAG CAATTTCAAT 
CCTTTACCTT ACTGGAATGT TGGTTGCCAG CTAGTGGCAC TAAACTACCA GTCTGAGGGA 
CGTATGATGC AATTAAACGA AGCAAAATTT AGGGTAAATG GCAACTGTGG TTACGTCCTC 
AAACCTCAGC AGATGTGCAA AGGCTCATTC AACCCCTATT CTTCAGATCC ACTTCCTGCT 
AGTCCTAAGA AGCAGCTCAT CCTGAAAATC ATCAGTGGAC AGCAGCTGCC TAAGCCTCCT 
GACTCAATGC TAGGAGACAG AGGAGAGATA ATAGATCCCT TTGTCGAGGT GGAAATTATT 
GGGTTACCTG TTGACTGCTG TAAAGATCAG ACCAGGGTGG TTGATGACAA TGGGTTTAAT 
CCTGTTTGGG AGGAAACGCT CACTTTTACC ATCCATATGC CTGAAATAGC TTTGGTTCGA 
TTTCTTGTTT GGGATCATGA CCCCATTGGG CGAGACTTCG TTGGACAAAG AACGCTGGCC 
TTTAGCAGTC TTGTACCTGG TTATCGACAT GTGTATTTAG AAGGGCTGAC AGAAGCTTCC 
ATATTTGTTC ACATAACCAT TAATGAAATC TATGGGAAGA GTAAGCAACT GATCGGACTC 
CGAGGGCTGT TTAACAAGAA CCCAAAGCAC AGTTCTGCTG AAAGCAGCGG TCATTATGTA 
CGGAAGCGTT CTATTGGTGA CCGAATTCTC CGGCGCACGG CCAGTGCTCC AGCAAAAGGT 
AGAAAGAAAA GCAAGATGGG TTTTCTGGAA GCTTCTGAAA TTAAAGACAG TGCATCCGAA 
CCAGCAGACC TGAAGGACAA AGAAGGAGTT GTAAGGCGTA CAAGCCGGAG TTTGCAAGCA 
CGTCCTGCTT CTATGCCAGT GGACAAATTC CTTCTTGTAG GACTGCCGTG CACAGAAGAT 
GAAACTGCTC AAGATGCCAA AGGAAAAGAC AGTGCAACTG CCAACAGTGA TGGCGATAAA 
ACTGAGAAGG AAACAAACTT GAAGGAATCT CTTTTCACGT GTTCTGAGAA AACTGCAGAT 
CATTCAAACT TGGCATCTCC CTTGAAAAAG GAGAGCGCCC AGAAGGAAAC TACAGCTGAT 
TTTTCTCTTG TACCCCAGGA GCAGCCCGAG CATTCGGTTG CTGTAAGTGA TGTAACCGAA 
ACAAACCGCC CCATAAGCAA TCAGGAAAGT CTCCTTCCTG CTGAAACGGT GCCTCTGATT 
CCGGACTCTG CCTTTGAGCG CATCACAGAA AAAATACAGG GCAAGAGCTG TGCAGGCAGT 
AGAAGGGCAG ATGACACAAA CAGGTGTGAG GAGAAAATAA CTCCTGTCAG GACTTGCCTT 
CCTGAAAAAG TAGAGGAGGT TGAAAATCTC AAATACGACA TCATCCACAG AAGCACTGTG 
GCGTGTCTTT CTGTGACAGA TAGTTCTCCT GCCGTCTACT CTGATGCTCC TGGTTTATGC 
TCCTCTTCAA CCATGCAAGA CAGCGACATC TCCCGCCTCA TAGACGAAGT TTCTTTGGTG 
ACTGAGAGCG AGATGGACAG CGCTGTCTCA GCTCTGATCG GCCAGCTGGA TGTCACCGAT 
GACCAAACCA GCCTCACCGC ACCGATGCTC CCTGGCGCTG GCAGCCGGGC CTTCAGTGCT 
GTGGCCACAC GCACACTGAC TGCAGAGCTC AGCACTCCCT GTAAGAAGGA CTTCATTGCC 
ACACAGTCCA GCAAGTCCCC GTTATTTTCA ACTCCAGACT CTGCCGTGTT CCCCAGCCCC 
GAGACTGCGG AGTATTCTGT GTACACAATT ATCCACGAGG CAACTCTTAC ACCCGGTTCT 
GAATTTAAGA CCCACAGTGG GTTAGTTTGC AAGAAGTCAA AGAGTGCAGA AAATAACTTG 
CCTAGCTCTT TTTGTTCTTC ACCTTTCTCT TTGCTGAATG CAAATCCCCA AAGAAAATGT 
GAAACAAGCT GGGAAGCCCC CGGCTCTGCG GGTTCTTTTG CTTCCAGTAG CCTCATCTTT 
GAGGAGATGC TTGTGGACCC TCCCATGGGC GAGAATTCGG AAAGCAGCAG TCTTGTAGAG 
TTAGGTGGGG ATTCCCAAGA CCTCTTGGTA ACTGCGTGCG CGTACAAAAG GGAAGATGTG 
AGCCAATTGG CTTCCCCTTT AAAACTGAGG CAGCAGCAGG ACAGCAGGTA CTGTGGTGAG 
AGGACCTTTG GAAACTGTGC AAGTGCTGGG CTCTGTGCTG CTGCCCTGGA CTGCTCGGAT 
AACTTTAGGA CTGCGGGAAG CAAGCTCCCT TTTCCAGCCT GTGAAAATGT TGGTTTTCTA 
CATCATCGTC ACACAGATGA GCAGAAAGAT TTGATGTGTA CCTCCAGGAG GTCCCAACTC 
GATGTGCACT CACCAGTGCT CAGAAGCGCG TTGGCTTTCC CACCCTCCCC AGCCATCAAA 
CAGACCAGCC CTTGCAAGTC CAAGAGCCTT GGTGACTTGA CATCGGAAGA TATTTCCTGC 
AATTTTGAAA GTAAGTATCA GTACATAAGT AGGAGCTTTG TCACATCTGG CATGAGAGAC 
AAGAAGCTCG CTGCCATGAA GACGATGAGA CCGCACTCTA CGGATGCTTT GACGGAACAG 
CTGAGAAAGC TGCTGTCCCT GGACCAGGAC GACAGCCAGC AGATGCTGTA TTCCAGGCGG 
CTCGACGAGG ACTGCCCAAA GGCACTGGTC AGAAAGCTGT CGTCCAGGAG CCAGAGCAGA 
GTGCGGAACA TCGCCAGCCG TGCCAAGGAG AGGCAGGAGG CCAGCAAGCA AAAATCTTCC 
ACCGCAGGCA GCATAGCGGG GGTCGTCCTT CGGAACAAGC CTTCGGCGTC CCCTCACGTC 
GTCAACAGGC ACTCGACGGG CTCCTACATA GCCAGGTACC TGGGCAGCTT CCCTGAGGAG 
GCAGCAGAGG GCCGGGGCCG ACCTGAGGGC ACGTGTGCTG CCTGGCACCG CGGCTGTGCT 
GAGCGGCTGT GTGCGCACAG CCCCCTGCTG CAGCTGGAGC CCGACGGCGA CGAGAAGCCT 
GAAGTCTATT TCCTGCTGAG GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004943619.1
CDS	1381..6447
Translation	MSYWSLDKKSCLQYCRHFLADNGVFHVERCMSVMQSGTQMVKLKSGTKGLVRLFYLDEHRTCIRWRPSRKSEKAKIVIDSIYKVTEGRQSEIFHRHAEGSFDPSCCFTIYHGNHLESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDHWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLNFEEFSVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTPEYCLDIIQKFEVSEENKEQNVLGIEGFTNFMRSPACDVFNPLHCEVHQDMDQPLCNYFIASSHNTYLTGDQLLSQSRVEMYARVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKIFFRDVAETINKYAFIKNEFPVILSIENHCSIQQQKKIAQYLKEILGDKLDLSSVVTGDSRQLPSPQNLKGKILVKGKKLPYSLGADAEEGEVSDEDSADEIEDDCKLKSCYSNGATEHQVESFIRTKLESLLKESQIRDKEDPDSFTVRALLRATHEGLSVNLKQNLDTKEGGKKSHSRSLMGNFGKHKKTGKATSKCSTSDDDENQQNSSGKEAGQLHRLTRRRKTVKLCRALSDLVVYTNSVAAQDIVDDGSTGNVLSFSETRAHQAVQQKAEQFMLYNQKQLTRVYPSAYRIDSSNFNPLPYWNVGCQLVALNYQSEGRMMQLNEAKFRVNGNCGYVLKPQQMCKGSFNPYSSDPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTIHMPEIALVRFLVWDHDPIGRDFVGQRTLAFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKSKQLIGLRGLFNKNPKHSSAESSGHYVRKRSIGDRILRRTASAPAKGRKKSKMGFLEASEIKDSASEPADLKDKEGVVRRTSRSLQARPASMPVDKFLLVGLPCTEDETAQDAKGKDSATANSDGDKTEKETNLKESLFTCSEKTADHSNLASPLKKESAQKETTADFSLVPQEQPEHSVAVSDVTETNRPISNQESLLPAETVPLIPDSAFERITEKIQGKSCAGSRRADDTNRCEEKITPVRTCLPEKVEEVENLKYDIIHRSTVACLSVTDSSPAVYSDAPGLCSSSTMQDSDISRLIDEVSLVTESEMDSAVSALIGQLDVTDDQTSLTAPMLPGAGSRAFSAVATRTLTAELSTPCKKDFIATQSSKSPLFSTPDSAVFPSPETAEYSVYTIIHEATLTPGSEFKTHSGLVCKKSKSAENNLPSSFCSSPFSLLNANPQRKCETSWEAPGSAGSFASSSLIFEEMLVDPPMGENSESSSLVELGGDSQDLLVTACAYKREDVSQLASPLKLRQQQDSRYCGERTFGNCASAGLCAAALDCSDNFRTAGSKLPFPACENVGFLHHRHTDEQKDLMCTSRRSQLDVHSPVLRSALAFPPSPAIKQTSPCKSKSLGDLTSEDISCNFESKYQYISRSFVTSGMRDKKLAAMKTMRPHSTDALTEQLRKLLSLDQDDSQQMLYSRRLDEDCPKALVRKLSSRSQSRVRNIASRAKERQEASKQKSSTAGSIAGVVLRNKPSASPHVVNRHSTGSYIARYLGSFPEEAAEGRGRPEGTCAAWHRGCAERLCAHSPLLQLEPDGDEKPEVYFLLRL

Target ORF information:

RefSeq Version	XM_004943562.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004943562.2

ORF Insert Sequence:

ATGAGTTACT GGAGTCTGGA TAAGAAAAGC TGTCTGCAGT ACTGCAGACA CTTCTTGGCA 
GACAACGGCG TGTTTCATGT TGAAAGATGC ATGAGTGTTA TGCAGTCTGG GACTCAGATG 
GTTAAGCTGA AGAGTGGAAC CAAAGGGCTC GTTCGTCTCT TCTACCTGGA CGAGCACAGG 
ACCTGCATCC GATGGAGACC GTCCAGAAAA AGTGAAAAGG CAAAAATTGT CATTGATTCT 
ATTTACAAAG TGACCGAAGG CCGTCAGTCT GAAATCTTCC ACCGCCATGC AGAGGGGAGC 
TTTGACCCGA GCTGTTGCTT TACCATTTAC CATGGCAACC ACCTGGAGTC ACTGGATCTG 
ATTACCTCTA ACCCAGAGGA AGCTCGCACC TGGATCACAG GGCTGAAATA TTTGATGGCT 
GGGATAAGCG ATGAGGACTC GCTTGCTAAG CGACAGAGAA CTCATGATCA CTGGGTGAAA 
CAAACTTTTG AGGAAGCTGA CAAGAATGGA GATGGCCTGC TGAACATTGA AGAGATCCAC 
CAGCTGATGC ATAAGCTAAA TGTTAACCTG CCCCGCAGGA AGGTCCGGCA GATGTTTCAG 
GAAGCTGACA CAGATGAGAA CCAAGGAACT CTAAATTTTG AAGAATTTTC AGTGTTTTAC 
AAGATGATGT CCTTGCGTCG AGACCTGTAC TTACTGCTCT TAAGCTACAG TGACAAGAAA 
GATCACCTCA CCGTTGAAGA ACTTGCTCAA TTTCTGAAGG TGGAACAAAA GATGAGTAAC 
GTGACGCCAG AATATTGTCT TGACATCATA CAGAAGTTTG AAGTATCAGA AGAAAACAAG 
GAGCAGAACG TGCTTGGGAT TGAAGGTTTC ACCAACTTCA TGCGCAGTCC TGCTTGTGAT 
GTTTTTAATC CATTGCATTG CGAAGTGCAT CAGGATATGG ATCAACCCCT TTGTAACTAC 
TTCATTGCTT CATCTCACAA CACGTACCTG ACAGGAGACC AGCTGCTGTC CCAGTCTAGG 
GTGGAGATGT ATGCACGAGT GCTGCAGGAC GGTTGTCGAT GTATTGAAGT GGATTGCTGG 
GATGGTCCAG ATGGAGAGCC AGTAGTGCAT CATGGGTATA CGCTTACCTC TAAAATATTC 
TTCCGTGACG TGGCAGAAAC TATCAATAAG TATGCCTTCA TCAAAAATGA GTTTCCGGTG 
ATACTGTCTA TTGAAAATCA TTGCAGTATT CAGCAGCAAA AGAAGATTGC TCAGTACTTG 
AAGGAGATAC TTGGTGACAA ATTGGACTTG TCATCTGTGG TCACCGGAGA TTCCAGGCAG 
CTTCCTAGCC CTCAAAACTT GAAAGGGAAA ATCCTAGTGA AGGGAAAAAA GTTGCCATAC 
AGTCTTGGAG CAGATGCTGA GGAAGGAGAA GTTTCTGATG AGGACAGTGC TGATGAAATT 
GAAGACGACT GCAAGCTGAA ATCCTGCTAT AGTAACGGTG CAACTGAGCA TCAGGTGGAA 
TCTTTTATAA GAACAAAGCT TGAATCCCTG CTAAAGGAGT CCCAGATCAG GGACAAAGAA 
GATCCTGACA GCTTCACGGT GAGAGCACTG CTAAGGGCAA CTCACGAGGG TTTAAGTGTT 
AACCTGAAGC AGAACCTGGA CACAAAAGAA GGTGGGAAGA AGTCTCATAG TCGATCATTA 
ATGGGCAACT TTGGTAAACA CAAAAAAACA GGGAAGGCAA CATCCAAATG TAGCACATCA 
GATGATGATG AAAATCAGCA AAACTCTTCT GGAAAGGAAG CAGGACAGCT GCACAGACTG 
ACTCGTCGAA GGAAAACTGT GAAGCTGTGC CGAGCTCTGT CTGACCTAGT GGTGTACACG 
AACTCTGTGG CAGCCCAGGA TATAGTAGAT GATGGATCAA CAGGGAATGT GCTGTCATTC 
AGTGAAACAA GAGCCCACCA AGCAGTGCAG CAGAAGGCTG AACAGTTCAT GCTTTACAAC 
CAGAAACAGC TTACAAGGGT TTATCCATCA GCGTATCGGA TTGACTCCAG CAATTTCAAT 
CCTTTACCTT ACTGGAATGT TGGTTGCCAG CTAGTGGCAC TAAACTACCA GTCTGAGGGA 
CGTATGATGC AATTAAACGA AGCAAAATTT AGGGTAAATG GCAACTGTGG TTACGTCCTC 
AAACCTCAGC AGATGTGCAA AGGCTCATTC AACCCCTATT CTTCAGATCC ACTTCCTGCT 
AGTCCTAAGA AGCAGCTCAT CCTGAAAATC ATCAGTGGAC AGCAGCTGCC TAAGCCTCCT 
GACTCAATGC TAGGAGACAG AGGAGAGATA ATAGATCCCT TTGTCGAGGT GGAAATTATT 
GGGTTACCTG TTGACTGCTG TAAAGATCAG ACCAGGGTGG TTGATGACAA TGGGTTTAAT 
CCTGTTTGGG AGGAAACGCT CACTTTTACC ATCCATATGC CTGAAATAGC TTTGGTTCGA 
TTTCTTGTTT GGGATCATGA CCCCATTGGG CGAGACTTCG TTGGACAAAG AACGCTGGCC 
TTTAGCAGTC TTGTACCTGG TTATCGACAT GTGTATTTAG AAGGGCTGAC AGAAGCTTCC 
ATATTTGTTC ACATAACCAT TAATGAAATC TATGGGAAGA GTAAGCAACT GATCGGACTC 
CGAGGGCTGT TTAACAAGAA CCCAAAGCAC AGTTCTGCTG AAAGCAGCGG TCATTATGTA 
CGGAAGCGTT CTATTGGTGA CCGAATTCTC CGGCGCACGG CCAGTGCTCC AGCAAAAGGT 
AGAAAGAAAA GCAAGATGGG TTTTCTGGAA GCTTCTGAAA TTAAAGACAG TGCATCCGAA 
CCAGCAGACC TGAAGGACAA AGAAGGAGTT GTAAGGCGTA CAAGCCGGAG TTTGCAAGCA 
CGTCCTGCTT CTATGCCAGT GGACAAATTC CTTCTTGTAG GACTGCCGTG CACAGAAGAT 
GAAACTGCTC AAGATGCCAA AGGAAAAGAC AGTGCAACTG CCAACAGTGA TGGCGATAAA 
ACTGAGAAGG AAACAAACTT GAAGGAATCT CTTTTCACGT GTTCTGAGAA AACTGCAGAT 
CATTCAAACT TGGCATCTCC CTTGAAAAAG GAGAGCGCCC AGAAGGAAAC TACAGCTGAT 
TTTTCTCTTG TACCCCAGGA GCAGCCCGAG CATTCGGTTG CTGTAAGTGA TGTAACCGAA 
ACAAACCGCC CCATAAGCAA TCAGGAAAGT CTCCTTCCTG CTGAAACGGT GCCTCTGATT 
CCGGACTCTG CCTTTGAGCG CATCACAGAA AAAATACAGG GCAAGAGCTG TGCAGGCAGT 
AGAAGGGCAG ATGACACAAA CAGGTGTGAG GAGAAAATAA CTCCTGTCAG GACTTGCCTT 
CCTGAAAAAG TAGAGGAGGT TGAAAATCTC AAATACGACA TCATCCACAG AAGCACTGTG 
GCGTGTCTTT CTGTGACAGA TAGTTCTCCT GCCGTCTACT CTGATGCTCC TGGTTTATGC 
TCCTCTTCAA CCATGCAAGA CAGCGACATC TCCCGCCTCA TAGACGAAGT TTCTTTGGTG 
ACTGAGAGCG AGATGGACAG CGCTGTCTCA GCTCTGATCG GCCAGCTGGA TGTCACCGAT 
GACCAAACCA GCCTCACCGC ACCGATGCTC CCTGGCGCTG GCAGCCGGGC CTTCAGTGCT 
GTGGCCACAC GCACACTGAC TGCAGAGCTC AGCACTCCCT GTAAGAAGGA CTTCATTGCC 
ACACAGTCCA GCAAGTCCCC GTTATTTTCA ACTCCAGACT CTGCCGTGTT CCCCAGCCCC 
GAGACTGCGG AGTATTCTGT GTACACAATT ATCCACGAGG CAACTCTTAC ACCCGGTTCT 
GAATTTAAGA CCCACAGTGG GTTAGTTTGC AAGAAGTCAA AGAGTGCAGA AAATAACTTG 
CCTAGCTCTT TTTGTTCTTC ACCTTTCTCT TTGCTGAATG CAAATCCCCA AAGAAAATGT 
GAAACAAGCT GGGAAGCCCC CGGCTCTGCG GGTTCTTTTG CTTCCAGTAG CCTCATCTTT 
GAGGAGATGC TTGTGGACCC TCCCATGGGC GAGAATTCGG AAAGCAGCAG TCTTGTAGAG 
TTAGGTGGGG ATTCCCAAGA CCTCTTGGTA ACTGCGTGCG CGTACAAAAG GGAAGATGTG 
AGCCAATTGG CTTCCCCTTT AAAACTGAGG CAGCAGCAGG ACAGCAGGTA CTGTGGTGAG 
AGGACCTTTG GAAACTGTGC AAGTGCTGGG CTCTGTGCTG CTGCCCTGGA CTGCTCGGAT 
AACTTTAGGA CTGCGGGAAG CAAGCTCCCT TTTCCAGCCT GTGAAAATGT TGGTTTTCTA 
CATCATCGTC ACACAGATGA GCAGAAAGAT TTGATGTGTA CCTCCAGGAG GTCCCAACTC 
GATGTGCACT CACCAGTGCT CAGAAGCGCG TTGGCTTTCC CACCCTCCCC AGCCATCAAA 
CAGACCAGCC CTTGCAAGTC CAAGAGCCTT GGTGACTTGA CATCGGAAGA TATTTCCTGC 
AATTTTGAAA GTAAGTATCA GTACATAAGT AGGAGCTTTG TCACATCTGG CATGAGAGAC 
AAGAAGCTCG CTGCCATGAA GACGATGAGA CCGCACTCTA CGGATGCTTT GACGGAACAG 
CTGAGAAAGC TGCTGTCCCT GGACCAGGAC GACAGCCAGC AGATGCTGTA TTCCAGGCGG 
CTCGACGAGG ACTGCCCAAA GGCACTGGTC AGAAAGCTGT CGTCCAGGAG CCAGAGCAGA 
GTGCGGAACA TCGCCAGCCG TGCCAAGGAG AGGCAGGAGG CCAGCAAGCA AAAATCTTCC 
ACCGCAGGCA GCATAGCGGG GGTCGTCCTT CGGAACAAGC CTTCGGCGTC CCCTCACGTC 
GTCAACAGGC ACTCGACGGG CTCCTACATA GCCAGGTACC TGGGCAGCTT CCCTGAGGAG 
GCAGCAGAGG GCCGGGGCCG ACCTGAGGGC ACGTGTGCTG CCTGGCACCG CGGCTGTGCT 
GAGCGGCTGT GTGCGCACAG CCCCCTGCTG CAGCTGGAGC CCGACGGCGA CGAGAAGCCT 
GAAGTCTATT TCCTGCTGAG GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_422832.4 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_422832.3 Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X7, mRNA.

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CloneID	OGa12868
Clone ID Related Accession (Same CDS sequence)	XM_422832.4 , XM_422832.5
Accession Version	XM_422832.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_422832.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACC TTGAAGTATA TAAAAATTTA AGTCCTGAAA AAGTTGAAAG ATGCATGAGT 
GTTATGCAGT CTGGGACTCA GATGGTTAAG CTGAAGAGTG GAACCAAAGG GCTCGTTCGT 
CTCTTCTACC TGGACGAGCA CAGGACCTGC ATCCGATGGA GACCGTCCAG AAAAAGTGAA 
AAGGCAAAAA TTGTCATTGA TTCTATTTAC AAAGTGACCG AAGGCCGTCA GTCTGAAATC 
TTCCACCGCC ATGCAGAGGG GAGCTTTGAC CCGAGCTGTT GCTTTACCAT TTACCATGGC 
AACCACCTGG AGTCACTGGA TCTGATTACC TCTAACCCAG AGGAAGCTCG CACCTGGATC 
ACAGGGCTGA AATATTTGAT GGCTGGGATA AGCGATGAGG ACTCGCTTGC TAAGCGACAG 
AGAACTCATG ATCACTGGGT GAAACAAACT TTTGAGGAAG CTGACAAGAA TGGAGATGGC 
CTGCTGAACA TTGAAGAGAT CCACCAGCTG ATGCATAAGC TAAATGTTAA CCTGCCCCGC 
AGGAAGGTCC GGCAGATGTT TCAGGAAGCT GACACAGATG AGAACCAAGG AACTCTAAAT 
TTTGAAGAAT TTTCAGTGTT TTACAAGATG ATGTCCTTGC GTCGAGACCT GTACTTACTG 
CTCTTAAGCT ACAGTGACAA GAAAGATCAC CTCACCGTTG AAGAACTTGC TCAATTTCTG 
AAGGTGGAAC AAAAGATGAG TAACGTGACG CCAGAATATT GTCTTGACAT CATACAGAAG 
TTTGAAGTAT CAGAAGAAAA CAAGGAGCAG AACGTGCTTG GGATTGAAGG TTTCACCAAC 
TTCATGCGCA GTCCTGCTTG TGATGTTTTT AATCCATTGC ATTGCGAAGT GCATCAGGAT 
ATGGATCAAC CCCTTTGTAA CTACTTCATT GCTTCATCTC ACAACACGTA CCTGACAGGA 
GACCAGCTGC TGTCCCAGTC TAGGGTGGAG ATGTATGCAC GAGTGCTGCA GGACGGTTGT 
CGATGTATTG AAGTGGATTG CTGGGATGGT CCAGATGGAG AGCCAGTAGT GCATCATGGG 
TATACGCTTA CCTCTAAAAT ATTCTTCCGT GACGTGGCAG AAACTATCAA TAAGTATGCC 
TTCATCAAAA ATGAGTTTCC GGTGATACTG TCTATTGAAA ATCATTGCAG TATTCAGCAG 
CAAAAGAAGA TTGCTCAGTA CTTGAAGGAG ATACTTGGTG ACAAATTGGA CTTGTCATCT 
GTGGTCACCG GAGATTCCAG GCAGCTTCCT AGCCCTCAAA ACTTGAAAGG GAAAATCCTA 
GTGAAGGGAA AAAAGTTGCC ATACAGTCTT GGAGCAGATG CTGAGGAAGG AGAAGTTTCT 
GATGAGGACA GTGCTGATGA AATTGAAGAC GACTGCAAGC TGAAATCCTG CTATAGTAAC 
GGTGCAACTG AGCATCAGGT GGAATCTTTT ATAAGAACAA AGCTTGAATC CCTGCTAAAG 
GAGTCCCAGA TCAGGGACAA AGAAGATCCT GACAGCTTCA CGGTGAGAGC ACTGCTAAGG 
GCAACTCACG AGGGTTTAAG TGTTAACCTG AAGCAGAACC TGGACACAAA AGAAGGTGGG 
AAGAAGTCTC ATAGTCGATC ATTAATGGGC AACTTTGGTA AACACAAAAA AACAGGGAAG 
GCAACATCCA AATGTAGCAC ATCAGATGAT GATGAAAATC AGCAAAACTC TTCTGGAAAG 
GAAGCAGGAC AGCTGCACAG ACTGACTCGT CGAAGGAAAA CTGTGAAGCT GTGCCGAGCT 
CTGTCTGACC TAGTGGTGTA CACGAACTCT GTGGCAGCCC AGGATATAGT AGATGATGGA 
TCAACAGGGA ATGTGCTGTC ATTCAGTGAA ACAAGAGCCC ACCAAGCAGT GCAGCAGAAG 
GCTGAACAGT TCATGCTTTA CAACCAGAAA CAGCTTACAA GGGTTTATCC ATCAGCGTAT 
CGGATTGACT CCAGCAATTT CAATCCTTTA CCTTACTGGA ATGTTGGTTG CCAGCTAGTG 
GCACTAAACT ACCAGTCTGA GGGACGTATG ATGCAATTAA ACGAAGCAAA ATTTAGGGTA 
AATGGCAACT GTGGTTACGT CCTCAAACCT CAGCAGATGT GCAAAGGCTC ATTCAACCCC 
TATTCTTCAG ATCCACTTCC TGCTAGTCCT AAGAAGCAGC TCATCCTGAA AATCATCAGT 
GGACAGCAGC TGCCTAAGCC TCCTGACTCA ATGCTAGGAG ACAGAGGAGA GATAATAGAT 
CCCTTTGTCG AGGTGGAAAT TATTGGGTTA CCTGTTGACT GCTGTAAAGA TCAGACCAGG 
GTGGTTGATG ACAATGGGTT TAATCCTGTT TGGGAGGAAA CGCTCACTTT TACCATCCAT 
ATGCCTGAAA TAGCTTTGGT TCGATTTCTT GTTTGGGATC ATGACCCCAT TGGGCGAGAC 
TTCGTTGGAC AAAGAACGCT GGCCTTTAGC AGTCTTGTAC CTGGTTATCG ACATGTGTAT 
TTAGAAGGGC TGACAGAAGC TTCCATATTT GTTCACATAA CCATTAATGA AATCTATGGG 
AAGTGGAGCC CCTTAATCCT CAATCCCAGT TACACAATAT TGCACTTTCT AGGAGCCACA 
AAGAGTAAGC AACTGATCGG ACTCCGAGGG CTGTTTAACA AGAACCCAAA GCACAGTTCT 
GCTGAAAGCA GCGGTCATTA TGTACGGAAG CGTTCTATTG GTGACCGAAT TCTCCGGCGC 
ACGGCCAGTG CTCCAGCAAA AGGTAGAAAG AAAAGCAAGA TGGGTTTTCT GGAAGCTTCT 
GAAATTAAAG ACAGTGCATC CGAACCAGCA GACCTGAAGG ACAAAGAAGG AGTTGTAAGG 
CGTACAAGCC GGAGTTTGCA AGCACGTCCT GCTTCTATGC CAGTGGACAA ATTCCTTCTT 
GTAGGACTGC CGTGCACAGA AGATGAAACT GCTCAAGATG CCAAAGGAAA AGACAGTGCA 
ACTGCCAACA GTGATGGCGA TAAAACTGAG AAGGAAACAA ACTTGAAGGA ATCTCTTTTC 
ACGTGTTCTG AGAAAACTGC AGATCATTCA AACTTGGCAT CTCCCTTGAA AAAGGAGAGC 
GCCCAGAAGG AAACTACAGC TGATTTTTCT CTTGTACCCC AGGAGCAGCC CGAGCATTCG 
GTTGCTGTAA GTGATGTAAC CGAAACAAAC CGCCCCATAA GCAATCAGGA AAGTCTCCTT 
CCTGCTGAAA CGGTGCCTCT GATTCCGGAC TCTGCCTTTG AGCGCATCAC AGAAAAAATA 
CAGGGCAAGA GCTGTGCAGG CAGTAGAAGG GCAGATGACA CAAACAGGTG TGAGGAGAAA 
ATAACTCCTG TCAGGACTTG CCTTCCTGAA AAAGTAGAGG AGGTTGAAAA TCTCAAATAC 
GACATCATCC ACAGAAGCAC TGTGGCGTGT CTTTCTGTGA CAGATAGTTC TCCTGCCGTC 
TACTCTGATG CTCCTGGTTT ATGCTCCTCT TCAACCATGC AAGACAGCGA CATCTCCCGC 
CTCATAGACG AAGTTTCTTT GGTGACTGAG AGCGAGATGG ACAGCGCTGT CTCAGCTCTG 
ATCGGCCAGC TGGATGTCAC CGATGACCAA ACCAGCCTCA CCGCACCGAT GCTCCCTGGC 
GCTGGCAGCC GGGCCTTCAG TGCTGTGGCC ACACGCACAC TGACTGCAGA GCTCAGCACT 
CCCTGTAAGA AGGACTTCAT TGCCACACAG TCCAGCAAGT CCCCGTTATT TTCAACTCCA 
GACTCTGCCG TGTTCCCCAG CCCCGAGACT GCGGAGTATT CTGTGTACAC AATTATCCAC 
GAGGCAACTC TTACACCCGG TTCTGAATTT AAGACCCACA GTGGGTTAGT TTGCAAGAAG 
TCAAAGAGTG CAGAAAATAA CTTGCCTAGC TCTTTTTGTT CTTCACCTTT CTCTTTGCTG 
AATGCAAATC CCCAAAGAAA ATGTGAAACA AGCTGGGAAG CCCCCGGCTC TGCGGGTTCT 
TTTGCTTCCA GTAGCCTCAT CTTTGAGGAG ATGCTTGTGG ACCCTCCCAT GGGCGAGAAT 
TCGGAAAGCA GCAGTCTTGT AGAGTTAGGT GGGGATTCCC AAGACCTCTT GGTAACTGCG 
TGCGCGTACA AAAGGGAAGA TGTGAGCCAA TTGGCTTCCC CTTTAAAACT GAGGCAGCAG 
CAGGACAGCA GGTACTGTGG TGAGAGGACC TTTGGAAACT GTGCAAGTGC TGGGCTCTGT 
GCTGCTGCCC TGGACTGCTC GGATAACTTT AGGACTGCGG GAAGCAAGCT CCCTTTTCCA 
GCCTGTGAAA ATGTTGGTTT TCTACATCAT CGTCACACAG ATGAGCAGAA AGATTTGATG 
TGTACCTCCA GGAGGTCCCA ACTCGATGTG CACTCACCAG TGCTCAGAAG CGCGTTGGCT 
TTCCCACCCT CCCCAGCCAT CAAACAGACC AGCCCTTGCA AGTCCAAGAG CCTTGGTGAC 
TTGACATCGG AAGATATTTC CTGCAATTTT GAAAGTAAGT ATCAGTACAT AAGTAGGAGC 
TTTGTCACAT CTGGCATGAG AGACAAGAAG CTCGCTGCCA TGAAGACGAT GAGACCGCAC 
TCTACGGATG CTTTGACGGA ACAGCTGAGA AAGCTGCTGT CCCTGGACCA GGACGACAGC 
CAGCAGATGC TGTATTCCAG GCGGCTCGAC GAGGACTGCC CAAAGGCACT GGTCAGAAAG 
CTGTCGTCCA GGAGCCAGAG CAGAGTGCGG AACATCGCCA GCCGTGCCAA GGAGAGGCAG 
GAGGCCAGCA AGCAAAAATC TTCCACCGCA GGCAGCATAG CGGGGGTCGT CCTTCGGAAC 
AAGCCTTCGG CGTCCCCTCA CGTCGTCAAC AGGCACTCGA CGGGCTCCTA CATAGCCAGG 
TACCTGGGCA GCTTCCCTGA GGAGGCAGCA GAGGGCCGGG GCCGACCTGA GGGCACGTGT 
GCTGCCTGGC ACCGCGGCTG TGCTGAGCGG CTGTGTGCGC ACAGCCCCCT GCTGCAGCTG 
GAGCCCGACG GCGACGAGAA GCCTGAAGTC TATTTCCTGC TGAGGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_422832.3
CDS	765..5855
Translation	MADLEVYKNLSPEKVERCMSVMQSGTQMVKLKSGTKGLVRLFYLDEHRTCIRWRPSRKSEKAKIVIDSIYKVTEGRQSEIFHRHAEGSFDPSCCFTIYHGNHLESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDHWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLNFEEFSVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTPEYCLDIIQKFEVSEENKEQNVLGIEGFTNFMRSPACDVFNPLHCEVHQDMDQPLCNYFIASSHNTYLTGDQLLSQSRVEMYARVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKIFFRDVAETINKYAFIKNEFPVILSIENHCSIQQQKKIAQYLKEILGDKLDLSSVVTGDSRQLPSPQNLKGKILVKGKKLPYSLGADAEEGEVSDEDSADEIEDDCKLKSCYSNGATEHQVESFIRTKLESLLKESQIRDKEDPDSFTVRALLRATHEGLSVNLKQNLDTKEGGKKSHSRSLMGNFGKHKKTGKATSKCSTSDDDENQQNSSGKEAGQLHRLTRRRKTVKLCRALSDLVVYTNSVAAQDIVDDGSTGNVLSFSETRAHQAVQQKAEQFMLYNQKQLTRVYPSAYRIDSSNFNPLPYWNVGCQLVALNYQSEGRMMQLNEAKFRVNGNCGYVLKPQQMCKGSFNPYSSDPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTIHMPEIALVRFLVWDHDPIGRDFVGQRTLAFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSKQLIGLRGLFNKNPKHSSAESSGHYVRKRSIGDRILRRTASAPAKGRKKSKMGFLEASEIKDSASEPADLKDKEGVVRRTSRSLQARPASMPVDKFLLVGLPCTEDETAQDAKGKDSATANSDGDKTEKETNLKESLFTCSEKTADHSNLASPLKKESAQKETTADFSLVPQEQPEHSVAVSDVTETNRPISNQESLLPAETVPLIPDSAFERITEKIQGKSCAGSRRADDTNRCEEKITPVRTCLPEKVEEVENLKYDIIHRSTVACLSVTDSSPAVYSDAPGLCSSSTMQDSDISRLIDEVSLVTESEMDSAVSALIGQLDVTDDQTSLTAPMLPGAGSRAFSAVATRTLTAELSTPCKKDFIATQSSKSPLFSTPDSAVFPSPETAEYSVYTIIHEATLTPGSEFKTHSGLVCKKSKSAENNLPSSFCSSPFSLLNANPQRKCETSWEAPGSAGSFASSSLIFEEMLVDPPMGENSESSSLVELGGDSQDLLVTACAYKREDVSQLASPLKLRQQQDSRYCGERTFGNCASAGLCAAALDCSDNFRTAGSKLPFPACENVGFLHHRHTDEQKDLMCTSRRSQLDVHSPVLRSALAFPPSPAIKQTSPCKSKSLGDLTSEDISCNFESKYQYISRSFVTSGMRDKKLAAMKTMRPHSTDALTEQLRKLLSLDQDDSQQMLYSRRLDEDCPKALVRKLSSRSQSRVRNIASRAKERQEASKQKSSTAGSIAGVVLRNKPSASPHVVNRHSTGSYIARYLGSFPEEAAEGRGRPEGTCAAWHRGCAERLCAHSPLLQLEPDGDEKPEVYFLLRL

Target ORF information:

RefSeq Version	XM_422832.4
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C, eta 1 (PLCH1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_422832.4

ORF Insert Sequence:

ATGGCAGACC TTGAAGTATA TAAAAATTTA AGTCCTGAAA AAGTTGAAAG ATGCATGAGT 
GTTATGCAGT CTGGGACTCA GATGGTTAAG CTGAAGAGTG GAACCAAAGG GCTCGTTCGT 
CTCTTCTACC TGGACGAGCA CAGGACCTGC ATCCGATGGA GACCGTCCAG AAAAAGTGAA 
AAGGCAAAAA TTGTCATTGA TTCTATTTAC AAAGTGACCG AAGGCCGTCA GTCTGAAATC 
TTCCACCGCC ATGCAGAGGG GAGCTTTGAC CCGAGCTGTT GCTTTACCAT TTACCATGGC 
AACCACCTGG AGTCACTGGA TCTGATTACC TCTAACCCAG AGGAAGCTCG CACCTGGATC 
ACAGGGCTGA AATATTTGAT GGCTGGGATA AGCGATGAGG ACTCGCTTGC TAAGCGACAG 
AGAACTCATG ATCACTGGGT GAAACAAACT TTTGAGGAAG CTGACAAGAA TGGAGATGGC 
CTGCTGAACA TTGAAGAGAT CCACCAGCTG ATGCATAAGC TAAATGTTAA CCTGCCCCGC 
AGGAAGGTCC GGCAGATGTT TCAGGAAGCT GACACAGATG AGAACCAAGG AACTCTAAAT 
TTTGAAGAAT TTTCAGTGTT TTACAAGATG ATGTCCTTGC GTCGAGACCT GTACTTACTG 
CTCTTAAGCT ACAGTGACAA GAAAGATCAC CTCACCGTTG AAGAACTTGC TCAATTTCTG 
AAGGTGGAAC AAAAGATGAG TAACGTGACG CCAGAATATT GTCTTGACAT CATACAGAAG 
TTTGAAGTAT CAGAAGAAAA CAAGGAGCAG AACGTGCTTG GGATTGAAGG TTTCACCAAC 
TTCATGCGCA GTCCTGCTTG TGATGTTTTT AATCCATTGC ATTGCGAAGT GCATCAGGAT 
ATGGATCAAC CCCTTTGTAA CTACTTCATT GCTTCATCTC ACAACACGTA CCTGACAGGA 
GACCAGCTGC TGTCCCAGTC TAGGGTGGAG ATGTATGCAC GAGTGCTGCA GGACGGTTGT 
CGATGTATTG AAGTGGATTG CTGGGATGGT CCAGATGGAG AGCCAGTAGT GCATCATGGG 
TATACGCTTA CCTCTAAAAT ATTCTTCCGT GACGTGGCAG AAACTATCAA TAAGTATGCC 
TTCATCAAAA ATGAGTTTCC GGTGATACTG TCTATTGAAA ATCATTGCAG TATTCAGCAG 
CAAAAGAAGA TTGCTCAGTA CTTGAAGGAG ATACTTGGTG ACAAATTGGA CTTGTCATCT 
GTGGTCACCG GAGATTCCAG GCAGCTTCCT AGCCCTCAAA ACTTGAAAGG GAAAATCCTA 
GTGAAGGGAA AAAAGTTGCC ATACAGTCTT GGAGCAGATG CTGAGGAAGG AGAAGTTTCT 
GATGAGGACA GTGCTGATGA AATTGAAGAC GACTGCAAGC TGAAATCCTG CTATAGTAAC 
GGTGCAACTG AGCATCAGGT GGAATCTTTT ATAAGAACAA AGCTTGAATC CCTGCTAAAG 
GAGTCCCAGA TCAGGGACAA AGAAGATCCT GACAGCTTCA CGGTGAGAGC ACTGCTAAGG 
GCAACTCACG AGGGTTTAAG TGTTAACCTG AAGCAGAACC TGGACACAAA AGAAGGTGGG 
AAGAAGTCTC ATAGTCGATC ATTAATGGGC AACTTTGGTA AACACAAAAA AACAGGGAAG 
GCAACATCCA AATGTAGCAC ATCAGATGAT GATGAAAATC AGCAAAACTC TTCTGGAAAG 
GAAGCAGGAC AGCTGCACAG ACTGACTCGT CGAAGGAAAA CTGTGAAGCT GTGCCGAGCT 
CTGTCTGACC TAGTGGTGTA CACGAACTCT GTGGCAGCCC AGGATATAGT AGATGATGGA 
TCAACAGGGA ATGTGCTGTC ATTCAGTGAA ACAAGAGCCC ACCAAGCAGT GCAGCAGAAG 
GCTGAACAGT TCATGCTTTA CAACCAGAAA CAGCTTACAA GGGTTTATCC ATCAGCGTAT 
CGGATTGACT CCAGCAATTT CAATCCTTTA CCTTACTGGA ATGTTGGTTG CCAGCTAGTG 
GCACTAAACT ACCAGTCTGA GGGACGTATG ATGCAATTAA ACGAAGCAAA ATTTAGGGTA 
AATGGCAACT GTGGTTACGT CCTCAAACCT CAGCAGATGT GCAAAGGCTC ATTCAACCCC 
TATTCTTCAG ATCCACTTCC TGCTAGTCCT AAGAAGCAGC TCATCCTGAA AATCATCAGT 
GGACAGCAGC TGCCTAAGCC TCCTGACTCA ATGCTAGGAG ACAGAGGAGA GATAATAGAT 
CCCTTTGTCG AGGTGGAAAT TATTGGGTTA CCTGTTGACT GCTGTAAAGA TCAGACCAGG 
GTGGTTGATG ACAATGGGTT TAATCCTGTT TGGGAGGAAA CGCTCACTTT TACCATCCAT 
ATGCCTGAAA TAGCTTTGGT TCGATTTCTT GTTTGGGATC ATGACCCCAT TGGGCGAGAC 
TTCGTTGGAC AAAGAACGCT GGCCTTTAGC AGTCTTGTAC CTGGTTATCG ACATGTGTAT 
TTAGAAGGGC TGACAGAAGC TTCCATATTT GTTCACATAA CCATTAATGA AATCTATGGG 
AAGTGGAGCC CCTTAATCCT CAATCCCAGT TACACAATAT TGCACTTTCT AGGAGCCACA 
AAGAGTAAGC AACTGATCGG ACTCCGAGGG CTGTTTAACA AGAACCCAAA GCACAGTTCT 
GCTGAAAGCA GCGGTCATTA TGTACGGAAG CGTTCTATTG GTGACCGAAT TCTCCGGCGC 
ACGGCCAGTG CTCCAGCAAA AGGTAGAAAG AAAAGCAAGA TGGGTTTTCT GGAAGCTTCT 
GAAATTAAAG ACAGTGCATC CGAACCAGCA GACCTGAAGG ACAAAGAAGG AGTTGTAAGG 
CGTACAAGCC GGAGTTTGCA AGCACGTCCT GCTTCTATGC CAGTGGACAA ATTCCTTCTT 
GTAGGACTGC CGTGCACAGA AGATGAAACT GCTCAAGATG CCAAAGGAAA AGACAGTGCA 
ACTGCCAACA GTGATGGCGA TAAAACTGAG AAGGAAACAA ACTTGAAGGA ATCTCTTTTC 
ACGTGTTCTG AGAAAACTGC AGATCATTCA AACTTGGCAT CTCCCTTGAA AAAGGAGAGC 
GCCCAGAAGG AAACTACAGC TGATTTTTCT CTTGTACCCC AGGAGCAGCC CGAGCATTCG 
GTTGCTGTAA GTGATGTAAC CGAAACAAAC CGCCCCATAA GCAATCAGGA AAGTCTCCTT 
CCTGCTGAAA CGGTGCCTCT GATTCCGGAC TCTGCCTTTG AGCGCATCAC AGAAAAAATA 
CAGGGCAAGA GCTGTGCAGG CAGTAGAAGG GCAGATGACA CAAACAGGTG TGAGGAGAAA 
ATAACTCCTG TCAGGACTTG CCTTCCTGAA AAAGTAGAGG AGGTTGAAAA TCTCAAATAC 
GACATCATCC ACAGAAGCAC TGTGGCGTGT CTTTCTGTGA CAGATAGTTC TCCTGCCGTC 
TACTCTGATG CTCCTGGTTT ATGCTCCTCT TCAACCATGC AAGACAGCGA CATCTCCCGC 
CTCATAGACG AAGTTTCTTT GGTGACTGAG AGCGAGATGG ACAGCGCTGT CTCAGCTCTG 
ATCGGCCAGC TGGATGTCAC CGATGACCAA ACCAGCCTCA CCGCACCGAT GCTCCCTGGC 
GCTGGCAGCC GGGCCTTCAG TGCTGTGGCC ACACGCACAC TGACTGCAGA GCTCAGCACT 
CCCTGTAAGA AGGACTTCAT TGCCACACAG TCCAGCAAGT CCCCGTTATT TTCAACTCCA 
GACTCTGCCG TGTTCCCCAG CCCCGAGACT GCGGAGTATT CTGTGTACAC AATTATCCAC 
GAGGCAACTC TTACACCCGG TTCTGAATTT AAGACCCACA GTGGGTTAGT TTGCAAGAAG 
TCAAAGAGTG CAGAAAATAA CTTGCCTAGC TCTTTTTGTT CTTCACCTTT CTCTTTGCTG 
AATGCAAATC CCCAAAGAAA ATGTGAAACA AGCTGGGAAG CCCCCGGCTC TGCGGGTTCT 
TTTGCTTCCA GTAGCCTCAT CTTTGAGGAG ATGCTTGTGG ACCCTCCCAT GGGCGAGAAT 
TCGGAAAGCA GCAGTCTTGT AGAGTTAGGT GGGGATTCCC AAGACCTCTT GGTAACTGCG 
TGCGCGTACA AAAGGGAAGA TGTGAGCCAA TTGGCTTCCC CTTTAAAACT GAGGCAGCAG 
CAGGACAGCA GGTACTGTGG TGAGAGGACC TTTGGAAACT GTGCAAGTGC TGGGCTCTGT 
GCTGCTGCCC TGGACTGCTC GGATAACTTT AGGACTGCGG GAAGCAAGCT CCCTTTTCCA 
GCCTGTGAAA ATGTTGGTTT TCTACATCAT CGTCACACAG ATGAGCAGAA AGATTTGATG 
TGTACCTCCA GGAGGTCCCA ACTCGATGTG CACTCACCAG TGCTCAGAAG CGCGTTGGCT 
TTCCCACCCT CCCCAGCCAT CAAACAGACC AGCCCTTGCA AGTCCAAGAG CCTTGGTGAC 
TTGACATCGG AAGATATTTC CTGCAATTTT GAAAGTAAGT ATCAGTACAT AAGTAGGAGC 
TTTGTCACAT CTGGCATGAG AGACAAGAAG CTCGCTGCCA TGAAGACGAT GAGACCGCAC 
TCTACGGATG CTTTGACGGA ACAGCTGAGA AAGCTGCTGT CCCTGGACCA GGACGACAGC 
CAGCAGATGC TGTATTCCAG GCGGCTCGAC GAGGACTGCC CAAAGGCACT GGTCAGAAAG 
CTGTCGTCCA GGAGCCAGAG CAGAGTGCGG AACATCGCCA GCCGTGCCAA GGAGAGGCAG 
GAGGCCAGCA AGCAAAAATC TTCCACCGCA GGCAGCATAG CGGGGGTCGT CCTTCGGAAC 
AAGCCTTCGG CGTCCCCTCA CGTCGTCAAC AGGCACTCGA CGGGCTCCTA CATAGCCAGG 
TACCTGGGCA GCTTCCCTGA GGAGGCAGCA GAGGGCCGGG GCCGACCTGA GGGCACGTGT 
GCTGCCTGGC ACCGCGGCTG TGCTGAGCGG CTGTGTGCGC ACAGCCCCCT GCTGCAGCTG 
GAGCCCGACG GCGACGAGAA GCCTGAAGTC TATTTCCTGC TGAGGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_422832.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_422832.3 Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X9, mRNA.

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CloneID	OGa12868
Clone ID Related Accession (Same CDS sequence)	XM_422832.4 , XM_422832.5
Accession Version	XM_422832.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_422832.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACC TTGAAGTATA TAAAAATTTA AGTCCTGAAA AAGTTGAAAG ATGCATGAGT 
GTTATGCAGT CTGGGACTCA GATGGTTAAG CTGAAGAGTG GAACCAAAGG GCTCGTTCGT 
CTCTTCTACC TGGACGAGCA CAGGACCTGC ATCCGATGGA GACCGTCCAG AAAAAGTGAA 
AAGGCAAAAA TTGTCATTGA TTCTATTTAC AAAGTGACCG AAGGCCGTCA GTCTGAAATC 
TTCCACCGCC ATGCAGAGGG GAGCTTTGAC CCGAGCTGTT GCTTTACCAT TTACCATGGC 
AACCACCTGG AGTCACTGGA TCTGATTACC TCTAACCCAG AGGAAGCTCG CACCTGGATC 
ACAGGGCTGA AATATTTGAT GGCTGGGATA AGCGATGAGG ACTCGCTTGC TAAGCGACAG 
AGAACTCATG ATCACTGGGT GAAACAAACT TTTGAGGAAG CTGACAAGAA TGGAGATGGC 
CTGCTGAACA TTGAAGAGAT CCACCAGCTG ATGCATAAGC TAAATGTTAA CCTGCCCCGC 
AGGAAGGTCC GGCAGATGTT TCAGGAAGCT GACACAGATG AGAACCAAGG AACTCTAAAT 
TTTGAAGAAT TTTCAGTGTT TTACAAGATG ATGTCCTTGC GTCGAGACCT GTACTTACTG 
CTCTTAAGCT ACAGTGACAA GAAAGATCAC CTCACCGTTG AAGAACTTGC TCAATTTCTG 
AAGGTGGAAC AAAAGATGAG TAACGTGACG CCAGAATATT GTCTTGACAT CATACAGAAG 
TTTGAAGTAT CAGAAGAAAA CAAGGAGCAG AACGTGCTTG GGATTGAAGG TTTCACCAAC 
TTCATGCGCA GTCCTGCTTG TGATGTTTTT AATCCATTGC ATTGCGAAGT GCATCAGGAT 
ATGGATCAAC CCCTTTGTAA CTACTTCATT GCTTCATCTC ACAACACGTA CCTGACAGGA 
GACCAGCTGC TGTCCCAGTC TAGGGTGGAG ATGTATGCAC GAGTGCTGCA GGACGGTTGT 
CGATGTATTG AAGTGGATTG CTGGGATGGT CCAGATGGAG AGCCAGTAGT GCATCATGGG 
TATACGCTTA CCTCTAAAAT ATTCTTCCGT GACGTGGCAG AAACTATCAA TAAGTATGCC 
TTCATCAAAA ATGAGTTTCC GGTGATACTG TCTATTGAAA ATCATTGCAG TATTCAGCAG 
CAAAAGAAGA TTGCTCAGTA CTTGAAGGAG ATACTTGGTG ACAAATTGGA CTTGTCATCT 
GTGGTCACCG GAGATTCCAG GCAGCTTCCT AGCCCTCAAA ACTTGAAAGG GAAAATCCTA 
GTGAAGGGAA AAAAGTTGCC ATACAGTCTT GGAGCAGATG CTGAGGAAGG AGAAGTTTCT 
GATGAGGACA GTGCTGATGA AATTGAAGAC GACTGCAAGC TGAAATCCTG CTATAGTAAC 
GGTGCAACTG AGCATCAGGT GGAATCTTTT ATAAGAACAA AGCTTGAATC CCTGCTAAAG 
GAGTCCCAGA TCAGGGACAA AGAAGATCCT GACAGCTTCA CGGTGAGAGC ACTGCTAAGG 
GCAACTCACG AGGGTTTAAG TGTTAACCTG AAGCAGAACC TGGACACAAA AGAAGGTGGG 
AAGAAGTCTC ATAGTCGATC ATTAATGGGC AACTTTGGTA AACACAAAAA AACAGGGAAG 
GCAACATCCA AATGTAGCAC ATCAGATGAT GATGAAAATC AGCAAAACTC TTCTGGAAAG 
GAAGCAGGAC AGCTGCACAG ACTGACTCGT CGAAGGAAAA CTGTGAAGCT GTGCCGAGCT 
CTGTCTGACC TAGTGGTGTA CACGAACTCT GTGGCAGCCC AGGATATAGT AGATGATGGA 
TCAACAGGGA ATGTGCTGTC ATTCAGTGAA ACAAGAGCCC ACCAAGCAGT GCAGCAGAAG 
GCTGAACAGT TCATGCTTTA CAACCAGAAA CAGCTTACAA GGGTTTATCC ATCAGCGTAT 
CGGATTGACT CCAGCAATTT CAATCCTTTA CCTTACTGGA ATGTTGGTTG CCAGCTAGTG 
GCACTAAACT ACCAGTCTGA GGGACGTATG ATGCAATTAA ACGAAGCAAA ATTTAGGGTA 
AATGGCAACT GTGGTTACGT CCTCAAACCT CAGCAGATGT GCAAAGGCTC ATTCAACCCC 
TATTCTTCAG ATCCACTTCC TGCTAGTCCT AAGAAGCAGC TCATCCTGAA AATCATCAGT 
GGACAGCAGC TGCCTAAGCC TCCTGACTCA ATGCTAGGAG ACAGAGGAGA GATAATAGAT 
CCCTTTGTCG AGGTGGAAAT TATTGGGTTA CCTGTTGACT GCTGTAAAGA TCAGACCAGG 
GTGGTTGATG ACAATGGGTT TAATCCTGTT TGGGAGGAAA CGCTCACTTT TACCATCCAT 
ATGCCTGAAA TAGCTTTGGT TCGATTTCTT GTTTGGGATC ATGACCCCAT TGGGCGAGAC 
TTCGTTGGAC AAAGAACGCT GGCCTTTAGC AGTCTTGTAC CTGGTTATCG ACATGTGTAT 
TTAGAAGGGC TGACAGAAGC TTCCATATTT GTTCACATAA CCATTAATGA AATCTATGGG 
AAGTGGAGCC CCTTAATCCT CAATCCCAGT TACACAATAT TGCACTTTCT AGGAGCCACA 
AAGAGTAAGC AACTGATCGG ACTCCGAGGG CTGTTTAACA AGAACCCAAA GCACAGTTCT 
GCTGAAAGCA GCGGTCATTA TGTACGGAAG CGTTCTATTG GTGACCGAAT TCTCCGGCGC 
ACGGCCAGTG CTCCAGCAAA AGGTAGAAAG AAAAGCAAGA TGGGTTTTCT GGAAGCTTCT 
GAAATTAAAG ACAGTGCATC CGAACCAGCA GACCTGAAGG ACAAAGAAGG AGTTGTAAGG 
CGTACAAGCC GGAGTTTGCA AGCACGTCCT GCTTCTATGC CAGTGGACAA ATTCCTTCTT 
GTAGGACTGC CGTGCACAGA AGATGAAACT GCTCAAGATG CCAAAGGAAA AGACAGTGCA 
ACTGCCAACA GTGATGGCGA TAAAACTGAG AAGGAAACAA ACTTGAAGGA ATCTCTTTTC 
ACGTGTTCTG AGAAAACTGC AGATCATTCA AACTTGGCAT CTCCCTTGAA AAAGGAGAGC 
GCCCAGAAGG AAACTACAGC TGATTTTTCT CTTGTACCCC AGGAGCAGCC CGAGCATTCG 
GTTGCTGTAA GTGATGTAAC CGAAACAAAC CGCCCCATAA GCAATCAGGA AAGTCTCCTT 
CCTGCTGAAA CGGTGCCTCT GATTCCGGAC TCTGCCTTTG AGCGCATCAC AGAAAAAATA 
CAGGGCAAGA GCTGTGCAGG CAGTAGAAGG GCAGATGACA CAAACAGGTG TGAGGAGAAA 
ATAACTCCTG TCAGGACTTG CCTTCCTGAA AAAGTAGAGG AGGTTGAAAA TCTCAAATAC 
GACATCATCC ACAGAAGCAC TGTGGCGTGT CTTTCTGTGA CAGATAGTTC TCCTGCCGTC 
TACTCTGATG CTCCTGGTTT ATGCTCCTCT TCAACCATGC AAGACAGCGA CATCTCCCGC 
CTCATAGACG AAGTTTCTTT GGTGACTGAG AGCGAGATGG ACAGCGCTGT CTCAGCTCTG 
ATCGGCCAGC TGGATGTCAC CGATGACCAA ACCAGCCTCA CCGCACCGAT GCTCCCTGGC 
GCTGGCAGCC GGGCCTTCAG TGCTGTGGCC ACACGCACAC TGACTGCAGA GCTCAGCACT 
CCCTGTAAGA AGGACTTCAT TGCCACACAG TCCAGCAAGT CCCCGTTATT TTCAACTCCA 
GACTCTGCCG TGTTCCCCAG CCCCGAGACT GCGGAGTATT CTGTGTACAC AATTATCCAC 
GAGGCAACTC TTACACCCGG TTCTGAATTT AAGACCCACA GTGGGTTAGT TTGCAAGAAG 
TCAAAGAGTG CAGAAAATAA CTTGCCTAGC TCTTTTTGTT CTTCACCTTT CTCTTTGCTG 
AATGCAAATC CCCAAAGAAA ATGTGAAACA AGCTGGGAAG CCCCCGGCTC TGCGGGTTCT 
TTTGCTTCCA GTAGCCTCAT CTTTGAGGAG ATGCTTGTGG ACCCTCCCAT GGGCGAGAAT 
TCGGAAAGCA GCAGTCTTGT AGAGTTAGGT GGGGATTCCC AAGACCTCTT GGTAACTGCG 
TGCGCGTACA AAAGGGAAGA TGTGAGCCAA TTGGCTTCCC CTTTAAAACT GAGGCAGCAG 
CAGGACAGCA GGTACTGTGG TGAGAGGACC TTTGGAAACT GTGCAAGTGC TGGGCTCTGT 
GCTGCTGCCC TGGACTGCTC GGATAACTTT AGGACTGCGG GAAGCAAGCT CCCTTTTCCA 
GCCTGTGAAA ATGTTGGTTT TCTACATCAT CGTCACACAG ATGAGCAGAA AGATTTGATG 
TGTACCTCCA GGAGGTCCCA ACTCGATGTG CACTCACCAG TGCTCAGAAG CGCGTTGGCT 
TTCCCACCCT CCCCAGCCAT CAAACAGACC AGCCCTTGCA AGTCCAAGAG CCTTGGTGAC 
TTGACATCGG AAGATATTTC CTGCAATTTT GAAAGTAAGT ATCAGTACAT AAGTAGGAGC 
TTTGTCACAT CTGGCATGAG AGACAAGAAG CTCGCTGCCA TGAAGACGAT GAGACCGCAC 
TCTACGGATG CTTTGACGGA ACAGCTGAGA AAGCTGCTGT CCCTGGACCA GGACGACAGC 
CAGCAGATGC TGTATTCCAG GCGGCTCGAC GAGGACTGCC CAAAGGCACT GGTCAGAAAG 
CTGTCGTCCA GGAGCCAGAG CAGAGTGCGG AACATCGCCA GCCGTGCCAA GGAGAGGCAG 
GAGGCCAGCA AGCAAAAATC TTCCACCGCA GGCAGCATAG CGGGGGTCGT CCTTCGGAAC 
AAGCCTTCGG CGTCCCCTCA CGTCGTCAAC AGGCACTCGA CGGGCTCCTA CATAGCCAGG 
TACCTGGGCA GCTTCCCTGA GGAGGCAGCA GAGGGCCGGG GCCGACCTGA GGGCACGTGT 
GCTGCCTGGC ACCGCGGCTG TGCTGAGCGG CTGTGTGCGC ACAGCCCCCT GCTGCAGCTG 
GAGCCCGACG GCGACGAGAA GCCTGAAGTC TATTTCCTGC TGAGGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_422832.3
CDS	629..5719
Translation	MADLEVYKNLSPEKVERCMSVMQSGTQMVKLKSGTKGLVRLFYLDEHRTCIRWRPSRKSEKAKIVIDSIYKVTEGRQSEIFHRHAEGSFDPSCCFTIYHGNHLESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDHWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLNFEEFSVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTPEYCLDIIQKFEVSEENKEQNVLGIEGFTNFMRSPACDVFNPLHCEVHQDMDQPLCNYFIASSHNTYLTGDQLLSQSRVEMYARVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKIFFRDVAETINKYAFIKNEFPVILSIENHCSIQQQKKIAQYLKEILGDKLDLSSVVTGDSRQLPSPQNLKGKILVKGKKLPYSLGADAEEGEVSDEDSADEIEDDCKLKSCYSNGATEHQVESFIRTKLESLLKESQIRDKEDPDSFTVRALLRATHEGLSVNLKQNLDTKEGGKKSHSRSLMGNFGKHKKTGKATSKCSTSDDDENQQNSSGKEAGQLHRLTRRRKTVKLCRALSDLVVYTNSVAAQDIVDDGSTGNVLSFSETRAHQAVQQKAEQFMLYNQKQLTRVYPSAYRIDSSNFNPLPYWNVGCQLVALNYQSEGRMMQLNEAKFRVNGNCGYVLKPQQMCKGSFNPYSSDPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTIHMPEIALVRFLVWDHDPIGRDFVGQRTLAFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSKQLIGLRGLFNKNPKHSSAESSGHYVRKRSIGDRILRRTASAPAKGRKKSKMGFLEASEIKDSASEPADLKDKEGVVRRTSRSLQARPASMPVDKFLLVGLPCTEDETAQDAKGKDSATANSDGDKTEKETNLKESLFTCSEKTADHSNLASPLKKESAQKETTADFSLVPQEQPEHSVAVSDVTETNRPISNQESLLPAETVPLIPDSAFERITEKIQGKSCAGSRRADDTNRCEEKITPVRTCLPEKVEEVENLKYDIIHRSTVACLSVTDSSPAVYSDAPGLCSSSTMQDSDISRLIDEVSLVTESEMDSAVSALIGQLDVTDDQTSLTAPMLPGAGSRAFSAVATRTLTAELSTPCKKDFIATQSSKSPLFSTPDSAVFPSPETAEYSVYTIIHEATLTPGSEFKTHSGLVCKKSKSAENNLPSSFCSSPFSLLNANPQRKCETSWEAPGSAGSFASSSLIFEEMLVDPPMGENSESSSLVELGGDSQDLLVTACAYKREDVSQLASPLKLRQQQDSRYCGERTFGNCASAGLCAAALDCSDNFRTAGSKLPFPACENVGFLHHRHTDEQKDLMCTSRRSQLDVHSPVLRSALAFPPSPAIKQTSPCKSKSLGDLTSEDISCNFESKYQYISRSFVTSGMRDKKLAAMKTMRPHSTDALTEQLRKLLSLDQDDSQQMLYSRRLDEDCPKALVRKLSSRSQSRVRNIASRAKERQEASKQKSSTAGSIAGVVLRNKPSASPHVVNRHSTGSYIARYLGSFPEEAAEGRGRPEGTCAAWHRGCAERLCAHSPLLQLEPDGDEKPEVYFLLRL

Target ORF information:

RefSeq Version	XM_422832.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C eta 1 (PLCH1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_422832.5

ORF Insert Sequence:

ATGGCAGACC TTGAAGTATA TAAAAATTTA AGTCCTGAAA AAGTTGAAAG ATGCATGAGT 
GTTATGCAGT CTGGGACTCA GATGGTTAAG CTGAAGAGTG GAACCAAAGG GCTCGTTCGT 
CTCTTCTACC TGGACGAGCA CAGGACCTGC ATCCGATGGA GACCGTCCAG AAAAAGTGAA 
AAGGCAAAAA TTGTCATTGA TTCTATTTAC AAAGTGACCG AAGGCCGTCA GTCTGAAATC 
TTCCACCGCC ATGCAGAGGG GAGCTTTGAC CCGAGCTGTT GCTTTACCAT TTACCATGGC 
AACCACCTGG AGTCACTGGA TCTGATTACC TCTAACCCAG AGGAAGCTCG CACCTGGATC 
ACAGGGCTGA AATATTTGAT GGCTGGGATA AGCGATGAGG ACTCGCTTGC TAAGCGACAG 
AGAACTCATG ATCACTGGGT GAAACAAACT TTTGAGGAAG CTGACAAGAA TGGAGATGGC 
CTGCTGAACA TTGAAGAGAT CCACCAGCTG ATGCATAAGC TAAATGTTAA CCTGCCCCGC 
AGGAAGGTCC GGCAGATGTT TCAGGAAGCT GACACAGATG AGAACCAAGG AACTCTAAAT 
TTTGAAGAAT TTTCAGTGTT TTACAAGATG ATGTCCTTGC GTCGAGACCT GTACTTACTG 
CTCTTAAGCT ACAGTGACAA GAAAGATCAC CTCACCGTTG AAGAACTTGC TCAATTTCTG 
AAGGTGGAAC AAAAGATGAG TAACGTGACG CCAGAATATT GTCTTGACAT CATACAGAAG 
TTTGAAGTAT CAGAAGAAAA CAAGGAGCAG AACGTGCTTG GGATTGAAGG TTTCACCAAC 
TTCATGCGCA GTCCTGCTTG TGATGTTTTT AATCCATTGC ATTGCGAAGT GCATCAGGAT 
ATGGATCAAC CCCTTTGTAA CTACTTCATT GCTTCATCTC ACAACACGTA CCTGACAGGA 
GACCAGCTGC TGTCCCAGTC TAGGGTGGAG ATGTATGCAC GAGTGCTGCA GGACGGTTGT 
CGATGTATTG AAGTGGATTG CTGGGATGGT CCAGATGGAG AGCCAGTAGT GCATCATGGG 
TATACGCTTA CCTCTAAAAT ATTCTTCCGT GACGTGGCAG AAACTATCAA TAAGTATGCC 
TTCATCAAAA ATGAGTTTCC GGTGATACTG TCTATTGAAA ATCATTGCAG TATTCAGCAG 
CAAAAGAAGA TTGCTCAGTA CTTGAAGGAG ATACTTGGTG ACAAATTGGA CTTGTCATCT 
GTGGTCACCG GAGATTCCAG GCAGCTTCCT AGCCCTCAAA ACTTGAAAGG GAAAATCCTA 
GTGAAGGGAA AAAAGTTGCC ATACAGTCTT GGAGCAGATG CTGAGGAAGG AGAAGTTTCT 
GATGAGGACA GTGCTGATGA AATTGAAGAC GACTGCAAGC TGAAATCCTG CTATAGTAAC 
GGTGCAACTG AGCATCAGGT GGAATCTTTT ATAAGAACAA AGCTTGAATC CCTGCTAAAG 
GAGTCCCAGA TCAGGGACAA AGAAGATCCT GACAGCTTCA CGGTGAGAGC ACTGCTAAGG 
GCAACTCACG AGGGTTTAAG TGTTAACCTG AAGCAGAACC TGGACACAAA AGAAGGTGGG 
AAGAAGTCTC ATAGTCGATC ATTAATGGGC AACTTTGGTA AACACAAAAA AACAGGGAAG 
GCAACATCCA AATGTAGCAC ATCAGATGAT GATGAAAATC AGCAAAACTC TTCTGGAAAG 
GAAGCAGGAC AGCTGCACAG ACTGACTCGT CGAAGGAAAA CTGTGAAGCT GTGCCGAGCT 
CTGTCTGACC TAGTGGTGTA CACGAACTCT GTGGCAGCCC AGGATATAGT AGATGATGGA 
TCAACAGGGA ATGTGCTGTC ATTCAGTGAA ACAAGAGCCC ACCAAGCAGT GCAGCAGAAG 
GCTGAACAGT TCATGCTTTA CAACCAGAAA CAGCTTACAA GGGTTTATCC ATCAGCGTAT 
CGGATTGACT CCAGCAATTT CAATCCTTTA CCTTACTGGA ATGTTGGTTG CCAGCTAGTG 
GCACTAAACT ACCAGTCTGA GGGACGTATG ATGCAATTAA ACGAAGCAAA ATTTAGGGTA 
AATGGCAACT GTGGTTACGT CCTCAAACCT CAGCAGATGT GCAAAGGCTC ATTCAACCCC 
TATTCTTCAG ATCCACTTCC TGCTAGTCCT AAGAAGCAGC TCATCCTGAA AATCATCAGT 
GGACAGCAGC TGCCTAAGCC TCCTGACTCA ATGCTAGGAG ACAGAGGAGA GATAATAGAT 
CCCTTTGTCG AGGTGGAAAT TATTGGGTTA CCTGTTGACT GCTGTAAAGA TCAGACCAGG 
GTGGTTGATG ACAATGGGTT TAATCCTGTT TGGGAGGAAA CGCTCACTTT TACCATCCAT 
ATGCCTGAAA TAGCTTTGGT TCGATTTCTT GTTTGGGATC ATGACCCCAT TGGGCGAGAC 
TTCGTTGGAC AAAGAACGCT GGCCTTTAGC AGTCTTGTAC CTGGTTATCG ACATGTGTAT 
TTAGAAGGGC TGACAGAAGC TTCCATATTT GTTCACATAA CCATTAATGA AATCTATGGG 
AAGTGGAGCC CCTTAATCCT CAATCCCAGT TACACAATAT TGCACTTTCT AGGAGCCACA 
AAGAGTAAGC AACTGATCGG ACTCCGAGGG CTGTTTAACA AGAACCCAAA GCACAGTTCT 
GCTGAAAGCA GCGGTCATTA TGTACGGAAG CGTTCTATTG GTGACCGAAT TCTCCGGCGC 
ACGGCCAGTG CTCCAGCAAA AGGTAGAAAG AAAAGCAAGA TGGGTTTTCT GGAAGCTTCT 
GAAATTAAAG ACAGTGCATC CGAACCAGCA GACCTGAAGG ACAAAGAAGG AGTTGTAAGG 
CGTACAAGCC GGAGTTTGCA AGCACGTCCT GCTTCTATGC CAGTGGACAA ATTCCTTCTT 
GTAGGACTGC CGTGCACAGA AGATGAAACT GCTCAAGATG CCAAAGGAAA AGACAGTGCA 
ACTGCCAACA GTGATGGCGA TAAAACTGAG AAGGAAACAA ACTTGAAGGA ATCTCTTTTC 
ACGTGTTCTG AGAAAACTGC AGATCATTCA AACTTGGCAT CTCCCTTGAA AAAGGAGAGC 
GCCCAGAAGG AAACTACAGC TGATTTTTCT CTTGTACCCC AGGAGCAGCC CGAGCATTCG 
GTTGCTGTAA GTGATGTAAC CGAAACAAAC CGCCCCATAA GCAATCAGGA AAGTCTCCTT 
CCTGCTGAAA CGGTGCCTCT GATTCCGGAC TCTGCCTTTG AGCGCATCAC AGAAAAAATA 
CAGGGCAAGA GCTGTGCAGG CAGTAGAAGG GCAGATGACA CAAACAGGTG TGAGGAGAAA 
ATAACTCCTG TCAGGACTTG CCTTCCTGAA AAAGTAGAGG AGGTTGAAAA TCTCAAATAC 
GACATCATCC ACAGAAGCAC TGTGGCGTGT CTTTCTGTGA CAGATAGTTC TCCTGCCGTC 
TACTCTGATG CTCCTGGTTT ATGCTCCTCT TCAACCATGC AAGACAGCGA CATCTCCCGC 
CTCATAGACG AAGTTTCTTT GGTGACTGAG AGCGAGATGG ACAGCGCTGT CTCAGCTCTG 
ATCGGCCAGC TGGATGTCAC CGATGACCAA ACCAGCCTCA CCGCACCGAT GCTCCCTGGC 
GCTGGCAGCC GGGCCTTCAG TGCTGTGGCC ACACGCACAC TGACTGCAGA GCTCAGCACT 
CCCTGTAAGA AGGACTTCAT TGCCACACAG TCCAGCAAGT CCCCGTTATT TTCAACTCCA 
GACTCTGCCG TGTTCCCCAG CCCCGAGACT GCGGAGTATT CTGTGTACAC AATTATCCAC 
GAGGCAACTC TTACACCCGG TTCTGAATTT AAGACCCACA GTGGGTTAGT TTGCAAGAAG 
TCAAAGAGTG CAGAAAATAA CTTGCCTAGC TCTTTTTGTT CTTCACCTTT CTCTTTGCTG 
AATGCAAATC CCCAAAGAAA ATGTGAAACA AGCTGGGAAG CCCCCGGCTC TGCGGGTTCT 
TTTGCTTCCA GTAGCCTCAT CTTTGAGGAG ATGCTTGTGG ACCCTCCCAT GGGCGAGAAT 
TCGGAAAGCA GCAGTCTTGT AGAGTTAGGT GGGGATTCCC AAGACCTCTT GGTAACTGCG 
TGCGCGTACA AAAGGGAAGA TGTGAGCCAA TTGGCTTCCC CTTTAAAACT GAGGCAGCAG 
CAGGACAGCA GGTACTGTGG TGAGAGGACC TTTGGAAACT GTGCAAGTGC TGGGCTCTGT 
GCTGCTGCCC TGGACTGCTC GGATAACTTT AGGACTGCGG GAAGCAAGCT CCCTTTTCCA 
GCCTGTGAAA ATGTTGGTTT TCTACATCAT CGTCACACAG ATGAGCAGAA AGATTTGATG 
TGTACCTCCA GGAGGTCCCA ACTCGATGTG CACTCACCAG TGCTCAGAAG CGCGTTGGCT 
TTCCCACCCT CCCCAGCCAT CAAACAGACC AGCCCTTGCA AGTCCAAGAG CCTTGGTGAC 
TTGACATCGG AAGATATTTC CTGCAATTTT GAAAGTAAGT ATCAGTACAT AAGTAGGAGC 
TTTGTCACAT CTGGCATGAG AGACAAGAAG CTCGCTGCCA TGAAGACGAT GAGACCGCAC 
TCTACGGATG CTTTGACGGA ACAGCTGAGA AAGCTGCTGT CCCTGGACCA GGACGACAGC 
CAGCAGATGC TGTATTCCAG GCGGCTCGAC GAGGACTGCC CAAAGGCACT GGTCAGAAAG 
CTGTCGTCCA GGAGCCAGAG CAGAGTGCGG AACATCGCCA GCCGTGCCAA GGAGAGGCAG 
GAGGCCAGCA AGCAAAAATC TTCCACCGCA GGCAGCATAG CGGGGGTCGT CCTTCGGAAC 
AAGCCTTCGG CGTCCCCTCA CGTCGTCAAC AGGCACTCGA CGGGCTCCTA CATAGCCAGG 
TACCTGGGCA GCTTCCCTGA GGAGGCAGCA GAGGGCCGGG GCCGACCTGA GGGCACGTGT 
GCTGCCTGGC ACCGCGGCTG TGCTGAGCGG CTGTGTGCGC ACAGCCCCCT GCTGCAGCTG 
GAGCCCGACG GCGACGAGAA GCCTGAAGTC TATTTCCTGC TGAGGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PLCH1
Entrez Gene ID	425030
Full Name	phospholipase C eta 1
Synonyms	PLCD4,PLCL3
General protein information	Preferred Names phospholipase C eta 1 Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 phospholipase C-like 3
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Pan troglodytes (chimpanzee)	PLCH1	XP_001149022.1
	LOC707243	XP_002808381.1
Bos taurus (cattle)	PLCH1	XP_002685035.3
Rattus norvegicus (Norway rat)	Plch1	NP_001178636.1
Xenopus tropicalis (tropical clawed frog)	plch1	XP_002933304.2
Homo sapiens (human)	PLCH1	NP_001124432.1
Gallus gallus (chicken)	PLCH1	XP_004943620.1
Danio rerio (zebrafish)	LOC566479	XP_694841.5
Mus musculus (house mouse)	Plch1	NP_899014.2
Canis lupus familiaris (dog)	PLCH1	XP_005634651.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PLCH1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

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