KALRN gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_015289978.2	XP_015145464.1	kalirin isoform X4
XM_015289980.2	XP_015145466.1	kalirin isoform X5
XM_015289982.2	XP_015145468.1	kalirin isoform X7
XM_015289974.1	XP_015145460.1	kalirin isoform X1
XM_015289980.1	XP_015145466.1	kalirin isoform X6
XM_015289984.1	XP_015145470.1	kalirin isoform X10
XM_015289981.2	XP_015145467.1	kalirin isoform X6
XM_015289984.2	XP_015145470.1	kalirin isoform X8
XM_025152648.1	XP_025008416.1	kalirin isoform X10
XM_015289978.1	XP_015145464.1	kalirin isoform X4
XM_015289974.2	XP_015145460.1	kalirin isoform X1
XM_015289976.1	XP_015145462.1	kalirin isoform X2
XM_015289982.1	XP_015145468.1	kalirin isoform X8
XM_015289983.1	XP_015145469.1	kalirin isoform X9
XM_015289985.1	XP_015145471.1	kalirin isoform X11
XM_015289985.2	XP_015145471.1	kalirin isoform X9
XM_015289977.1	XP_015145463.1	kalirin isoform X3
XM_015289979.1	XP_015145465.1	kalirin isoform X5
XM_015289981.1	XP_015145467.1	kalirin isoform X7
XM_015289987.1	XP_015145473.1	kalirin isoform X12
XM_015289976.2	XP_015145462.1	kalirin isoform X2
XM_015289977.2	XP_015145463.1	kalirin isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa152169	XM_025152648.1 Latest version!	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa38681	XM_015289985.2 Latest version!	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa38680	XM_015289984.2 Latest version!	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa38679	XM_015289983.1 Latest version!	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa38671	XM_015289974.2 Latest version!	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OGa38672	XM_015289976.2 Latest version!	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OGa38673	XM_015289977.2 Latest version!	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OGa38674	XM_015289978.2 Latest version!	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OGa38675	XM_015289979.1 Latest version!	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OGa38676	XM_015289980.2 Latest version!	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OGa38677	XM_015289981.2 Latest version!	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OGa38678	XM_015289982.2 Latest version!	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OGa38682	XM_015289987.1 Latest version!	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OGa38676	XM_015289980.1	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OGa38680	XM_015289984.1	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa38674	XM_015289978.1	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OGa38671	XM_015289974.1	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OGa38678	XM_015289982.1	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OGa38681	XM_015289985.1	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa38677	XM_015289981.1	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OGa38672	XM_015289976.1	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OGa38673	XM_015289977.1	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

KALRN ( XM_025152648.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025008416.1 Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OGa152169
Clone ID Related Accession (Same CDS sequence)	XM_025152648.1
Accession Version	XM_025152648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	kalirin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGCT TCTTCCCTTC AGGAAAAGAG GCATATTCAG TCTCTTCCAA TGAAAACGGG 
GGCAAGTCCG ATTCAGTGGC CAACTTACAG CCCCAGCCAT CCCTCAACTC CATCCAGAGC 
TCTCCCGGCC CCAAGCGCTC GACCAACACG CTGAAGAAAT GGCTGACGAG CCCCGTGCGC 
CGGCTGAACA GCGGGAAGGC CGAGAGCAAC ATCAAGAAGC AAAAGAAGGT GCGAGATGGC 
AGGAAGAGCT TTGACCTCGG CTCGCCGAAG ACTGGAGATG AAACCACTCC TCAAGGAGAC 
AGCGCGGATG AGAAGAGCAA GAAGGGTTGG GGAGAAGATG AGCCAGATGA AGAGTCTCAC 
ACACCTCTTC CTCCTCCTAT GAAGATTTTT GACAATGATC CAACCCAAGA TGAGATGTCC 
TCTTCTTTGC TAGCTGCTCG GCAGAGCTCC TCCGATGTCC CAACTGCTGC GGATCTTGTC 
AGTGCAATAG AACAGTTGGT CAAAAGTAAG CTGACCCTTG AAGGAGGATC TTACCGAGGA 
AGCTTAAAAG ATGCCACAGG CTGCTTGAAT GAAGGAATGG CGCCTTCAAC TCCCCCAAGA 
AACCTTGAAG AGGAACAAAA AGCCAAAGCA ATGAGAGGCA GGATGTTTGT CTTAAATGAA 
CTGGTCCAGA CTGAAAAAGA CTACGTCAAA GACTTGGGAA CCGTTGTGGA GGGCTTCATG 
AAGAGAATAG AAGAAAAGGG AGTTTCCGAA GATATGAAAG GGAAAGACAA GATAGTGTTT 
GGCAATATTC ACCAGATCTA TGACTGGCAC AAAGACTTTT TCCTGGCTGA ACTGGAAAAA 
TGTCTTCAGG AGCCTGACCG TTTAGCACAG CTTTTTATTA AACATGAGCG GCGATTACAC 
ATGTATGTGG TGTACTGCCA AAACAAGCCA CGATCTGAAT ATATAGTAGC TGAATACGAG 
GCATATTTTG AGGAGGTTCA ACAGGAAATA AACCAACGGC TGACCATCAG TGACTTTCTG 
ATCAAACCCA TTCAGAGAAT AACTAAATAT CAGCTTCTTC TCAAGGACTT CCTGAGGTAC 
AGTGAAAAAG CTGGTCTGGA GTGCTCTGAA ATAGAGAAAG CAGTTGAGTT AATGTGCCTT 
GTACCAAAAC GTTGCAATGA TATGATGAAC CTGGGTCGCC TCCAAGGCTT TGAGGGTAAG 
CTGACAGCCC AGGGCAAGCT GCTGCAGCAG GACACCTTCT ACGTGACAGA GCATGACTCC 
GGAGTTCAGT CCCGACCAAA GGAACGTAGG GTGTTTCTCT TTGAGCAAAT AGTTATCTTC 
AGCGAGCTGC TTAGGAAAGG GTCTTTAACA CCAGGCTACA TGTTCAAGAG AAGCATAAAG 
ATGAACTACC TTGTCATTGA AGAAAATGTG GATGGTGATC CCTGCAAGTT TGCTCTAATG 
AATCGGGAAA CATCTGAGAA AGTTGTTTTA CAGGCAGCTA ATCCAGAAAT TCAGCAGGCT 
TGGGTACAGG ACATCAACCA GGTCCTGGAC ACACAAAGGG ATTTCCTAAA TGCACTGCAG 
TCTCCCATAG AGTACCAACG CAAAGAAAGT AGCACAGCAG TGCTGAGGCC CCAGGCTGGT 
AGGGTCGCTC AGCCCAACAG CAGACCCTAT TCTTCTGTTC CAGTAGGGTC TGAGAAGCCT 
TTGAAGGCTA CAAGCCGTAA CCCATCTCTC CCACCCCTGA AGATATCTAC CTCCAACGGC 
AGTACAGGGT ATGAACAGCC CGGAGACAAG TATGAACAAA GCAAGACTGA TTTGGGAGGG 
TGCAATGGGA CATCTTCCAT GGTGGTGATT AAAGATTACT ATGCAGTGAA GGAGGACGAG 
ATCTGTGTGA ATCAAGGAGA GGTGGTTCAG ATCCTTGCTA TCAACCAGCA AAACATGTTC 
CTGGTGTACC AGCCTGCAAA TGACCACTCT CCAGCTGCTG AAGGATGGAT CCCTGGCAAT 
ATCTTGGCTC CCCTCACTAA ACCCACTACA GATAATAGCG ACGGAAGCAT CAAGAAGTCA 
TGTTCATGGC ATACCCTGCG CATGAGAAAG CGGGCGGAAA AGGAGAGCAG TGGCAAAAAT 
GAAGCCAATG GGCCACGTAA ATCCAAGGAT ATTCTGGGGA ACAAAGTCTC TGTTAAAGAG 
ACAAACAGCT CAGAGGAATC AGAGTGTGAT GACCTTGACC CCAATACTAG CATGGAGATA 
ATCAACCCCA ATTTCATTCA GGAAGTGGCT CCAGAGTTCC TTGTGCTGTT GGTGGATATC 
ACCTGCCTGC TTGGCGACAC TGTGATGCTG CAGTGCAAAG TCTGCGGCCG GCCCAAACCA 
ACAATAACCT GGAAAGGACC TGATCAAAAC ATTCTTGACA ATGACAACAG CACGGCCACA 
TACACGGTGT CATCCTGTGA CTCTGGAGAG CTCATGCTGA AGATCTGCAA TGTGATGCCT 
CAGGACAGTG GCATTTACAC TTGTGTGGCA ACTAACGAGC ATGGAACGGC ATCAACCTCA 
GCCACAATCA AAGTGCAAGG TGTACCGGCT GCCCCAAATC GCCCCATTGC TCAGGAGAGA 
AGCTGCACCT CTGTGATCCT TCGCTGGCTG CCTCCATCCA GTACAGGCAA TTGCACCATT 
TCTGGCTACA CAGTGGAGTA CAGAGAAGAA GGCTCACAGG TCTGGCAGCA GTCAGTGGCC 
TCCACACTGG ACACTTACCT TGTCATTGAA GACCTGTCCC CAGGCTGCCA GTATCAGTTC 
CGTGTGAGTG CCAGCAACCC CTGGGGGATC AGCTTGCCCA GTGACCCATC TGAATTCGTG 
AGGCTTCCAG AGTATGATTC TGCTGCTGAT GGTGCCACTA TTTCGTGGAA GGAAAACTTT 
GATCTCGCTT ATGCGGAGCT GCATGAGATT GGGAGGGGAC GATTCTCCAT TGTAAAGAAA 
TGCGTTCACA AAGCTACCCG GAAAGACGTG GCTGTAAAAT TCATCAGTAA AAAAATGAAG 
AAGAAGGAGC AAGCAGCCCA CGAGGCAGCT CTGTTACAGC ACTTACAGCA TCCTCAGTAC 
ATCACTATCC ATGATACCTA TGAGTCTCCT ACTTCTTACA TCCTAGTCCT GGAGCTGATG 
GATGATGGTC GTCTCCTAGA TTATCTAATG AACCATGATG AACTTATGGA GGAAAAAGTA 
GCTTTCTACA TAAGAGACAC AATGGAAGCC TTGCAGTATC TTCACAATTG CCGAGTGGCT 
CATTTAGATA TAAAGCCAGA GAATCTGCTC ATCGATTTAA GGATCCCAGT TCCTCGTGTC 
AAGATCATTG ATTTGGAAGA TGCAGTTCAA ATCACAGGCC ATTACCACGT CCACCATCTC 
CTTGGAAACC CAGAGTTTGC AGCCCCTGAA GTTATCCAGG GCCTCCCCGT GTCCCTGAGC 
ACAGACATCT GGAGCATCGG GGTCCTCACC TACGTCATGC TGAGCGGTGT CTCCCCGTTC 
CTGGATGAAA GCAAAGAGGA GACGTGTATC AATGTGTGCA GAGTCGATTT CAGCTTCCCG 
CCCGAGTACT TCTCTGATGT GAGCCATGCT GCTAGGGATT TCATCAACGT CATCCTCCAG 
GAAGACTCCA GGAGGAGGCC GACAGCAGCC ACTTGTTTAC AGCACCCATG GCTGCAGCCA 
CACAATGGCA GCTACTCCAA AATCCCGCTG GATACCTCCC GCTTGGCATC CTTCATTGAG 
CGCCGCAGAC ACCAGTACGA CGTGCACCCC GTCCCCGGTG TCAAGAGTTT CCTGATGAGC 
AGGCTGAACC CCGGGACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025008416.1
CDS	265..4065
Translation	MDCFFPSGKEAYSVSSNENGGKSDSVANLQPQPSLNSIQSSPGPKRSTNTLKKWLTSPVRRLNSGKAESNIKKQKKVRDGRKSFDLGSPKTGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSSLLAARQSSSDVPTAADLVSAIEQLVKSKLTLEGGSYRGSLKDATGCLNEGMAPSTPPRNLEEEQKAKAMRGRMFVLNELVQTEKDYVKDLGTVVEGFMKRIEEKGVSEDMKGKDKIVFGNIHQIYDWHKDFFLAELEKCLQEPDRLAQLFIKHERRLHMYVVYCQNKPRSEYIVAEYEAYFEEVQQEINQRLTISDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSEIEKAVELMCLVPKRCNDMMNLGRLQGFEGKLTAQGKLLQQDTFYVTEHDSGVQSRPKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVIEENVDGDPCKFALMNRETSEKVVLQAANPEIQQAWVQDINQVLDTQRDFLNALQSPIEYQRKESSTAVLRPQAGRVAQPNSRPYSSVPVGSEKPLKATSRNPSLPPLKISTSNGSTGYEQPGDKYEQSKTDLGGCNGTSSMVVIKDYYAVKEDEICVNQGEVVQILAINQQNMFLVYQPANDHSPAAEGWIPGNILAPLTKPTTDNSDGSIKKSCSWHTLRMRKRAEKESSGKNEANGPRKSKDILGNKVSVKETNSSEESECDDLDPNTSMEIINPNFIQEVAPEFLVLLVDITCLLGDTVMLQCKVCGRPKPTITWKGPDQNILDNDNSTATYTVSSCDSGELMLKICNVMPQDSGIYTCVATNEHGTASTSATIKVQGVPAAPNRPIAQERSCTSVILRWLPPSSTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCQYQFRVSASNPWGISLPSDPSEFVRLPEYDSAADGATISWKENFDLAYAELHEIGRGRFSIVKKCVHKATRKDVAVKFISKKMKKKEQAAHEAALLQHLQHPQYITIHDTYESPTSYILVLELMDDGRLLDYLMNHDELMEEKVAFYIRDTMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKIIDLEDAVQITGHYHVHHLLGNPEFAAPEVIQGLPVSLSTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPPEYFSDVSHAARDFINVILQEDSRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLASFIERRRHQYDVHPVPGVKSFLMSRLNPGT

Target ORF information:

RefSeq Version	XM_025152648.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025152648.1

ORF Insert Sequence:

ATGGATTGCT TCTTCCCTTC AGGAAAAGAG GCATATTCAG TCTCTTCCAA TGAAAACGGG 
GGCAAGTCCG ATTCAGTGGC CAACTTACAG CCCCAGCCAT CCCTCAACTC CATCCAGAGC 
TCTCCCGGCC CCAAGCGCTC GACCAACACG CTGAAGAAAT GGCTGACGAG CCCCGTGCGC 
CGGCTGAACA GCGGGAAGGC CGAGAGCAAC ATCAAGAAGC AAAAGAAGGT GCGAGATGGC 
AGGAAGAGCT TTGACCTCGG CTCGCCGAAG ACTGGAGATG AAACCACTCC TCAAGGAGAC 
AGCGCGGATG AGAAGAGCAA GAAGGGTTGG GGAGAAGATG AGCCAGATGA AGAGTCTCAC 
ACACCTCTTC CTCCTCCTAT GAAGATTTTT GACAATGATC CAACCCAAGA TGAGATGTCC 
TCTTCTTTGC TAGCTGCTCG GCAGAGCTCC TCCGATGTCC CAACTGCTGC GGATCTTGTC 
AGTGCAATAG AACAGTTGGT CAAAAGTAAG CTGACCCTTG AAGGAGGATC TTACCGAGGA 
AGCTTAAAAG ATGCCACAGG CTGCTTGAAT GAAGGAATGG CGCCTTCAAC TCCCCCAAGA 
AACCTTGAAG AGGAACAAAA AGCCAAAGCA ATGAGAGGCA GGATGTTTGT CTTAAATGAA 
CTGGTCCAGA CTGAAAAAGA CTACGTCAAA GACTTGGGAA CCGTTGTGGA GGGCTTCATG 
AAGAGAATAG AAGAAAAGGG AGTTTCCGAA GATATGAAAG GGAAAGACAA GATAGTGTTT 
GGCAATATTC ACCAGATCTA TGACTGGCAC AAAGACTTTT TCCTGGCTGA ACTGGAAAAA 
TGTCTTCAGG AGCCTGACCG TTTAGCACAG CTTTTTATTA AACATGAGCG GCGATTACAC 
ATGTATGTGG TGTACTGCCA AAACAAGCCA CGATCTGAAT ATATAGTAGC TGAATACGAG 
GCATATTTTG AGGAGGTTCA ACAGGAAATA AACCAACGGC TGACCATCAG TGACTTTCTG 
ATCAAACCCA TTCAGAGAAT AACTAAATAT CAGCTTCTTC TCAAGGACTT CCTGAGGTAC 
AGTGAAAAAG CTGGTCTGGA GTGCTCTGAA ATAGAGAAAG CAGTTGAGTT AATGTGCCTT 
GTACCAAAAC GTTGCAATGA TATGATGAAC CTGGGTCGCC TCCAAGGCTT TGAGGGTAAG 
CTGACAGCCC AGGGCAAGCT GCTGCAGCAG GACACCTTCT ACGTGACAGA GCATGACTCC 
GGAGTTCAGT CCCGACCAAA GGAACGTAGG GTGTTTCTCT TTGAGCAAAT AGTTATCTTC 
AGCGAGCTGC TTAGGAAAGG GTCTTTAACA CCAGGCTACA TGTTCAAGAG AAGCATAAAG 
ATGAACTACC TTGTCATTGA AGAAAATGTG GATGGTGATC CCTGCAAGTT TGCTCTAATG 
AATCGGGAAA CATCTGAGAA AGTTGTTTTA CAGGCAGCTA ATCCAGAAAT TCAGCAGGCT 
TGGGTACAGG ACATCAACCA GGTCCTGGAC ACACAAAGGG ATTTCCTAAA TGCACTGCAG 
TCTCCCATAG AGTACCAACG CAAAGAAAGT AGCACAGCAG TGCTGAGGCC CCAGGCTGGT 
AGGGTCGCTC AGCCCAACAG CAGACCCTAT TCTTCTGTTC CAGTAGGGTC TGAGAAGCCT 
TTGAAGGCTA CAAGCCGTAA CCCATCTCTC CCACCCCTGA AGATATCTAC CTCCAACGGC 
AGTACAGGGT ATGAACAGCC CGGAGACAAG TATGAACAAA GCAAGACTGA TTTGGGAGGG 
TGCAATGGGA CATCTTCCAT GGTGGTGATT AAAGATTACT ATGCAGTGAA GGAGGACGAG 
ATCTGTGTGA ATCAAGGAGA GGTGGTTCAG ATCCTTGCTA TCAACCAGCA AAACATGTTC 
CTGGTGTACC AGCCTGCAAA TGACCACTCT CCAGCTGCTG AAGGATGGAT CCCTGGCAAT 
ATCTTGGCTC CCCTCACTAA ACCCACTACA GATAATAGCG ACGGAAGCAT CAAGAAGTCA 
TGTTCATGGC ATACCCTGCG CATGAGAAAG CGGGCGGAAA AGGAGAGCAG TGGCAAAAAT 
GAAGCCAATG GGCCACGTAA ATCCAAGGAT ATTCTGGGGA ACAAAGTCTC TGTTAAAGAG 
ACAAACAGCT CAGAGGAATC AGAGTGTGAT GACCTTGACC CCAATACTAG CATGGAGATA 
ATCAACCCCA ATTTCATTCA GGAAGTGGCT CCAGAGTTCC TTGTGCTGTT GGTGGATATC 
ACCTGCCTGC TTGGCGACAC TGTGATGCTG CAGTGCAAAG TCTGCGGCCG GCCCAAACCA 
ACAATAACCT GGAAAGGACC TGATCAAAAC ATTCTTGACA ATGACAACAG CACGGCCACA 
TACACGGTGT CATCCTGTGA CTCTGGAGAG CTCATGCTGA AGATCTGCAA TGTGATGCCT 
CAGGACAGTG GCATTTACAC TTGTGTGGCA ACTAACGAGC ATGGAACGGC ATCAACCTCA 
GCCACAATCA AAGTGCAAGG TGTACCGGCT GCCCCAAATC GCCCCATTGC TCAGGAGAGA 
AGCTGCACCT CTGTGATCCT TCGCTGGCTG CCTCCATCCA GTACAGGCAA TTGCACCATT 
TCTGGCTACA CAGTGGAGTA CAGAGAAGAA GGCTCACAGG TCTGGCAGCA GTCAGTGGCC 
TCCACACTGG ACACTTACCT TGTCATTGAA GACCTGTCCC CAGGCTGCCA GTATCAGTTC 
CGTGTGAGTG CCAGCAACCC CTGGGGGATC AGCTTGCCCA GTGACCCATC TGAATTCGTG 
AGGCTTCCAG AGTATGATTC TGCTGCTGAT GGTGCCACTA TTTCGTGGAA GGAAAACTTT 
GATCTCGCTT ATGCGGAGCT GCATGAGATT GGGAGGGGAC GATTCTCCAT TGTAAAGAAA 
TGCGTTCACA AAGCTACCCG GAAAGACGTG GCTGTAAAAT TCATCAGTAA AAAAATGAAG 
AAGAAGGAGC AAGCAGCCCA CGAGGCAGCT CTGTTACAGC ACTTACAGCA TCCTCAGTAC 
ATCACTATCC ATGATACCTA TGAGTCTCCT ACTTCTTACA TCCTAGTCCT GGAGCTGATG 
GATGATGGTC GTCTCCTAGA TTATCTAATG AACCATGATG AACTTATGGA GGAAAAAGTA 
GCTTTCTACA TAAGAGACAC AATGGAAGCC TTGCAGTATC TTCACAATTG CCGAGTGGCT 
CATTTAGATA TAAAGCCAGA GAATCTGCTC ATCGATTTAA GGATCCCAGT TCCTCGTGTC 
AAGATCATTG ATTTGGAAGA TGCAGTTCAA ATCACAGGCC ATTACCACGT CCACCATCTC 
CTTGGAAACC CAGAGTTTGC AGCCCCTGAA GTTATCCAGG GCCTCCCCGT GTCCCTGAGC 
ACAGACATCT GGAGCATCGG GGTCCTCACC TACGTCATGC TGAGCGGTGT CTCCCCGTTC 
CTGGATGAAA GCAAAGAGGA GACGTGTATC AATGTGTGCA GAGTCGATTT CAGCTTCCCG 
CCCGAGTACT TCTCTGATGT GAGCCATGCT GCTAGGGATT TCATCAACGT CATCCTCCAG 
GAAGACTCCA GGAGGAGGCC GACAGCAGCC ACTTGTTTAC AGCACCCATG GCTGCAGCCA 
CACAATGGCA GCTACTCCAA AATCCCGCTG GATACCTCCC GCTTGGCATC CTTCATTGAG 
CGCCGCAGAC ACCAGTACGA CGTGCACCCC GTCCCCGGTG TCAAGAGTTT CCTGATGAGC 
AGGCTGAACC CCGGGACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KALRN ( XM_015289985.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145471.1 Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OGa38681
Clone ID Related Accession (Same CDS sequence)	XM_015289985.1 , XM_015289985.2
Accession Version	XM_015289985.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	kalirin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGCGG GCAGGCGCGG CTATCCAGCC CGCTCCGGTG TAGGATGGCT CTTGGCGAAG 
TGCTGCTGCT GCTCCTGCAA AGAGGCATAT TCAGTCTCTT CCAATGAAAA CGGGGGCAAG 
TCCGATTCAG TGGCCAACTT ACAGCCCCAG CCATCCCTCA ACTCCATCCA GAGCTCTCCC 
GGCCCCAAGC GCTCGACCAA CACGCTGAAG AAATGGCTGA CGAGCCCCGT GCGCCGGCTG 
AACAGCGGGA AGGCCGAGAG CAACATCAAG AAGCAAAAGA AGGTGCGAGA TGGCAGGAAG 
AGCTTTGACC TCGGCTCGCC GAAGACTGGA GATGAAACCA CTCCTCAAGG AGACAGCGCG 
GATGAGAAGA GCAAGAAGGG TTGGGGAGAA GATGAGCCAG ATGAAGAGTC TCACACACCT 
CTTCCTCCTC CTATGAAGAT TTTTGACAAT GATCCAACCC AAGATGAGAT GTCCTCTTCT 
TTGCTAGCTG CTCGGCAGAG CTCCTCCGAT GTCCCAACTG CTGCGGATCT TGTCAGTGCA 
ATAGAACAGT TGGTCAAAAG TAAGCTGACC CTTGAAGGAG GATCTTACCG AGGAAGCTTA 
AAAGATGCCA CAGGCTGCTT GAATGAAGGA ATGGCGCCTT CAACTCCCCC AAGAAACCTT 
GAAGAGGAAC AAAAAGCCAA AGCAATGAGA GGCAGGATGT TTGTCTTAAA TGAACTGGTC 
CAGACTGAAA AAGACTACGT CAAAGACTTG GGAACCGTTG TGGAGGGCTT CATGAAGAGA 
ATAGAAGAAA AGGGAGTTTC CGAAGATATG AAAGGGAAAG ACAAGATAGT GTTTGGCAAT 
ATTCACCAGA TCTATGACTG GCACAAAGAC TTTTTCCTGG CTGAACTGGA AAAATGTCTT 
CAGGAGCCTG ACCGTTTAGC ACAGCTTTTT ATTAAACATG AGCGGCGATT ACACATGTAT 
GTGGTGTACT GCCAAAACAA GCCACGATCT GAATATATAG TAGCTGAATA CGAGGCATAT 
TTTGAGGAGG TTCAACAGGA AATAAACCAA CGGCTGACCA TCAGTGACTT TCTGATCAAA 
CCCATTCAGA GAATAACTAA ATATCAGCTT CTTCTCAAGG ACTTCCTGAG GTACAGTGAA 
AAAGCTGGTC TGGAGTGCTC TGAAATAGAG AAAGCAGTTG AGTTAATGTG CCTTGTACCA 
AAACGTTGCA ATGATATGAT GAACCTGGGT CGCCTCCAAG GCTTTGAGGG TAAGCTGACA 
GCCCAGGGCA AGCTGCTGCA GCAGGACACC TTCTACGTGA CAGAGCATGA CTCCGGAGTT 
CAGTCCCGAC CAAAGGAACG TAGGGTGTTT CTCTTTGAGC AAATAGTTAT CTTCAGCGAG 
CTGCTTAGGA AAGGGTCTTT AACACCAGGC TACATGTTCA AGAGAAGCAT AAAGATGAAC 
TACCTTGTCA TTGAAGAAAA TGTGGATGGT GATCCCTGCA AGTTTGCTCT AATGAATCGG 
GAAACATCTG AGAAAGTTGT TTTACAGGCA GCTAATCCAG AAATTCAGCA GGCTTGGGTA 
CAGGACATCA ACCAGGTCCT GGACACACAA AGGGATTTCC TAAATGCACT GCAGTCTCCC 
ATAGAGTACC AACGCAAAGA AAGTAGCACA GCAGTGCTGA GGCCCCAGGC TGGTAGGGTC 
GCTCAGCCCA ACAGCAGACC CTATTCTTCT GTTCCAGTAG GGTCTGAGAA GCCTTTGAAG 
GCTACAAGCC GTAACCCATC TCTCCCACCC CTGAAGATAT CTACCTCCAA CGGCAGTACA 
GGGTATGAAC AGCCCGGAGA CAAGTATGAA CAAAGCAAGA CTGATTTGGG AGGGTGCAAT 
GGGACATCTT CCATGGTGGT GATTAAAGAT TACTATGCAG TGAAGGAGGA CGAGATCTGT 
GTGAATCAAG GAGAGGTGGT TCAGATCCTT GCTATCAACC AGCAAAACAT GTTCCTGGTG 
TACCAGCCTG CAAATGACCA CTCTCCAGCT GCTGAAGGAT GGATCCCTGG CAATATCTTG 
GCTCCCCTCA CTAAACCCAC TACAGATAAT AGCGACGGAA GCATCAAGAA GTCATGTTCA 
TGGCATACCC TGCGCATGAG AAAGCGGGCG GAAAAGGAGA GCAGTGGCAA AAATGAAGCC 
AATGGGCCAC GTAAATCCAA GGATATTCTG GGGAACAAAG TCTCTGTTAA AGAGACAAAC 
AGCTCAGAGG AATCAGAGTG TGATGACCTT GACCCCAATA CTAGCATGGA GATAATCAAC 
CCCAATTTCA TTCAGGAAGT GGCTCCAGAG TTCCTTGTGC TGTTGGTGGA TATCACCTGC 
CTGCTTGGCG ACACTGTGAT GCTGCAGTGC AAAGTCTGCG GCCGGCCCAA ACCAACAATA 
ACCTGGAAAG GACCTGATCA AAACATTCTT GACAATGACA ACAGCACGGC CACATACACG 
GTGTCATCCT GTGACTCTGG AGAGCTCATG CTGAAGATCT GCAATGTGAT GCCTCAGGAC 
AGTGGCATTT ACACTTGTGT GGCAACTAAC GAGCATGGAA CGGCATCAAC CTCAGCCACA 
ATCAAAGTGC AAGGTGTACC GGCTGCCCCA AATCGCCCCA TTGCTCAGGA GAGAAGCTGC 
ACCTCTGTGA TCCTTCGCTG GCTGCCTCCA TCCAGTACAG GCAATTGCAC CATTTCTGGC 
TACACAGTGG AGTACAGAGA AGAAGGCTCA CAGGTCTGGC AGCAGTCAGT GGCCTCCACA 
CTGGACACTT ACCTTGTCAT TGAAGACCTG TCCCCAGGCT GCCAGTATCA GTTCCGTGTG 
AGTGCCAGCA ACCCCTGGGG GATCAGCTTG CCCAGTGACC CATCTGAATT CGTGAGGCTT 
CCAGAGTATG ATTCTGCTGC TGATGGTGCC ACTATTTCGT GGAAGGAAAA CTTTGATCTC 
GCTTATGCGG AGCTGCATGA GATTGGGAGG GGACGATTCT CCATTGTAAA GAAATGCGTT 
CACAAAGCTA CCCGGAAAGA CGTGGCTGTA AAATTCATCA GTAAAAAAAT GAAGAAGAAG 
GAGCAAGCAG CCCACGAGGC AGCTCTGTTA CAGCACTTAC AGCATCCTCA GTACATCACT 
ATCCATGATA CCTATGAGTC TCCTACTTCT TACATCCTAG TCCTGGAGCT GATGGATGAT 
GGTCGTCTCC TAGATTATCT AATGAACCAT GATGAACTTA TGGAGGAAAA AGTAGCTTTC 
TACATAAGAG ACACAATGGA AGCCTTGCAG TATCTTCACA ATTGCCGAGT GGCTCATTTA 
GATATAAAGC CAGAGAATCT GCTCATCGAT TTAAGGATCC CAGTTCCTCG TGTCAAGATC 
ATTGATTTGG AAGATGCAGT TCAAATCACA GGCCATTACC ACGTCCACCA TCTCCTTGGA 
AACCCAGAGT TTGCAGCCCC TGAAGTTATC CAGGGCCTCC CCGTGTCCCT GAGCACAGAC 
ATCTGGAGCA TCGGGGTCCT CACCTACGTC ATGCTGAGCG GTGTCTCCCC GTTCCTGGAT 
GAAAGCAAAG AGGAGACGTG TATCAATGTG TGCAGAGTCG ATTTCAGCTT CCCGCCCGAG 
TACTTCTCTG ATGTGAGCCA TGCTGCTAGG GATTTCATCA ACGTCATCCT CCAGGAAGAC 
TCCAGGAGGA GGCCGACAGC AGCCACTTGT TTACAGCACC CATGGCTGCA GCCACACAAT 
GGCAGCTACT CCAAAATCCC GCTGGATACC TCCCGCTTGG CATCCTTCAT TGAGCGCCGC 
AGACACCAGT ACGACGTGCA CCCCGTCCCC GGTGTCAAGA GTTTCCTGAT GAGCAGGCTG 
AACCCCGGGA CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145471.1
CDS	184..4038
Translation	MKAGRRGYPARSGVGWLLAKCCCCSCKEAYSVSSNENGGKSDSVANLQPQPSLNSIQSSPGPKRSTNTLKKWLTSPVRRLNSGKAESNIKKQKKVRDGRKSFDLGSPKTGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSSLLAARQSSSDVPTAADLVSAIEQLVKSKLTLEGGSYRGSLKDATGCLNEGMAPSTPPRNLEEEQKAKAMRGRMFVLNELVQTEKDYVKDLGTVVEGFMKRIEEKGVSEDMKGKDKIVFGNIHQIYDWHKDFFLAELEKCLQEPDRLAQLFIKHERRLHMYVVYCQNKPRSEYIVAEYEAYFEEVQQEINQRLTISDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSEIEKAVELMCLVPKRCNDMMNLGRLQGFEGKLTAQGKLLQQDTFYVTEHDSGVQSRPKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVIEENVDGDPCKFALMNRETSEKVVLQAANPEIQQAWVQDINQVLDTQRDFLNALQSPIEYQRKESSTAVLRPQAGRVAQPNSRPYSSVPVGSEKPLKATSRNPSLPPLKISTSNGSTGYEQPGDKYEQSKTDLGGCNGTSSMVVIKDYYAVKEDEICVNQGEVVQILAINQQNMFLVYQPANDHSPAAEGWIPGNILAPLTKPTTDNSDGSIKKSCSWHTLRMRKRAEKESSGKNEANGPRKSKDILGNKVSVKETNSSEESECDDLDPNTSMEIINPNFIQEVAPEFLVLLVDITCLLGDTVMLQCKVCGRPKPTITWKGPDQNILDNDNSTATYTVSSCDSGELMLKICNVMPQDSGIYTCVATNEHGTASTSATIKVQGVPAAPNRPIAQERSCTSVILRWLPPSSTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCQYQFRVSASNPWGISLPSDPSEFVRLPEYDSAADGATISWKENFDLAYAELHEIGRGRFSIVKKCVHKATRKDVAVKFISKKMKKKEQAAHEAALLQHLQHPQYITIHDTYESPTSYILVLELMDDGRLLDYLMNHDELMEEKVAFYIRDTMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKIIDLEDAVQITGHYHVHHLLGNPEFAAPEVIQGLPVSLSTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPPEYFSDVSHAARDFINVILQEDSRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLASFIERRRHQYDVHPVPGVKSFLMSRLNPGT

Target ORF information:

RefSeq Version	XM_015289985.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289985.1

ORF Insert Sequence:

ATGAAAGCGG GCAGGCGCGG CTATCCAGCC CGCTCCGGTG TAGGATGGCT CTTGGCGAAG 
TGCTGCTGCT GCTCCTGCAA AGAGGCATAT TCAGTCTCTT CCAATGAAAA CGGGGGCAAG 
TCCGATTCAG TGGCCAACTT ACAGCCCCAG CCATCCCTCA ACTCCATCCA GAGCTCTCCC 
GGCCCCAAGC GCTCGACCAA CACGCTGAAG AAATGGCTGA CGAGCCCCGT GCGCCGGCTG 
AACAGCGGGA AGGCCGAGAG CAACATCAAG AAGCAAAAGA AGGTGCGAGA TGGCAGGAAG 
AGCTTTGACC TCGGCTCGCC GAAGACTGGA GATGAAACCA CTCCTCAAGG AGACAGCGCG 
GATGAGAAGA GCAAGAAGGG TTGGGGAGAA GATGAGCCAG ATGAAGAGTC TCACACACCT 
CTTCCTCCTC CTATGAAGAT TTTTGACAAT GATCCAACCC AAGATGAGAT GTCCTCTTCT 
TTGCTAGCTG CTCGGCAGAG CTCCTCCGAT GTCCCAACTG CTGCGGATCT TGTCAGTGCA 
ATAGAACAGT TGGTCAAAAG TAAGCTGACC CTTGAAGGAG GATCTTACCG AGGAAGCTTA 
AAAGATGCCA CAGGCTGCTT GAATGAAGGA ATGGCGCCTT CAACTCCCCC AAGAAACCTT 
GAAGAGGAAC AAAAAGCCAA AGCAATGAGA GGCAGGATGT TTGTCTTAAA TGAACTGGTC 
CAGACTGAAA AAGACTACGT CAAAGACTTG GGAACCGTTG TGGAGGGCTT CATGAAGAGA 
ATAGAAGAAA AGGGAGTTTC CGAAGATATG AAAGGGAAAG ACAAGATAGT GTTTGGCAAT 
ATTCACCAGA TCTATGACTG GCACAAAGAC TTTTTCCTGG CTGAACTGGA AAAATGTCTT 
CAGGAGCCTG ACCGTTTAGC ACAGCTTTTT ATTAAACATG AGCGGCGATT ACACATGTAT 
GTGGTGTACT GCCAAAACAA GCCACGATCT GAATATATAG TAGCTGAATA CGAGGCATAT 
TTTGAGGAGG TTCAACAGGA AATAAACCAA CGGCTGACCA TCAGTGACTT TCTGATCAAA 
CCCATTCAGA GAATAACTAA ATATCAGCTT CTTCTCAAGG ACTTCCTGAG GTACAGTGAA 
AAAGCTGGTC TGGAGTGCTC TGAAATAGAG AAAGCAGTTG AGTTAATGTG CCTTGTACCA 
AAACGTTGCA ATGATATGAT GAACCTGGGT CGCCTCCAAG GCTTTGAGGG TAAGCTGACA 
GCCCAGGGCA AGCTGCTGCA GCAGGACACC TTCTACGTGA CAGAGCATGA CTCCGGAGTT 
CAGTCCCGAC CAAAGGAACG TAGGGTGTTT CTCTTTGAGC AAATAGTTAT CTTCAGCGAG 
CTGCTTAGGA AAGGGTCTTT AACACCAGGC TACATGTTCA AGAGAAGCAT AAAGATGAAC 
TACCTTGTCA TTGAAGAAAA TGTGGATGGT GATCCCTGCA AGTTTGCTCT AATGAATCGG 
GAAACATCTG AGAAAGTTGT TTTACAGGCA GCTAATCCAG AAATTCAGCA GGCTTGGGTA 
CAGGACATCA ACCAGGTCCT GGACACACAA AGGGATTTCC TAAATGCACT GCAGTCTCCC 
ATAGAGTACC AACGCAAAGA AAGTAGCACA GCAGTGCTGA GGCCCCAGGC TGGTAGGGTC 
GCTCAGCCCA ACAGCAGACC CTATTCTTCT GTTCCAGTAG GGTCTGAGAA GCCTTTGAAG 
GCTACAAGCC GTAACCCATC TCTCCCACCC CTGAAGATAT CTACCTCCAA CGGCAGTACA 
GGGTATGAAC AGCCCGGAGA CAAGTATGAA CAAAGCAAGA CTGATTTGGG AGGGTGCAAT 
GGGACATCTT CCATGGTGGT GATTAAAGAT TACTATGCAG TGAAGGAGGA CGAGATCTGT 
GTGAATCAAG GAGAGGTGGT TCAGATCCTT GCTATCAACC AGCAAAACAT GTTCCTGGTG 
TACCAGCCTG CAAATGACCA CTCTCCAGCT GCTGAAGGAT GGATCCCTGG CAATATCTTG 
GCTCCCCTCA CTAAACCCAC TACAGATAAT AGCGACGGAA GCATCAAGAA GTCATGTTCA 
TGGCATACCC TGCGCATGAG AAAGCGGGCG GAAAAGGAGA GCAGTGGCAA AAATGAAGCC 
AATGGGCCAC GTAAATCCAA GGATATTCTG GGGAACAAAG TCTCTGTTAA AGAGACAAAC 
AGCTCAGAGG AATCAGAGTG TGATGACCTT GACCCCAATA CTAGCATGGA GATAATCAAC 
CCCAATTTCA TTCAGGAAGT GGCTCCAGAG TTCCTTGTGC TGTTGGTGGA TATCACCTGC 
CTGCTTGGCG ACACTGTGAT GCTGCAGTGC AAAGTCTGCG GCCGGCCCAA ACCAACAATA 
ACCTGGAAAG GACCTGATCA AAACATTCTT GACAATGACA ACAGCACGGC CACATACACG 
GTGTCATCCT GTGACTCTGG AGAGCTCATG CTGAAGATCT GCAATGTGAT GCCTCAGGAC 
AGTGGCATTT ACACTTGTGT GGCAACTAAC GAGCATGGAA CGGCATCAAC CTCAGCCACA 
ATCAAAGTGC AAGGTGTACC GGCTGCCCCA AATCGCCCCA TTGCTCAGGA GAGAAGCTGC 
ACCTCTGTGA TCCTTCGCTG GCTGCCTCCA TCCAGTACAG GCAATTGCAC CATTTCTGGC 
TACACAGTGG AGTACAGAGA AGAAGGCTCA CAGGTCTGGC AGCAGTCAGT GGCCTCCACA 
CTGGACACTT ACCTTGTCAT TGAAGACCTG TCCCCAGGCT GCCAGTATCA GTTCCGTGTG 
AGTGCCAGCA ACCCCTGGGG GATCAGCTTG CCCAGTGACC CATCTGAATT CGTGAGGCTT 
CCAGAGTATG ATTCTGCTGC TGATGGTGCC ACTATTTCGT GGAAGGAAAA CTTTGATCTC 
GCTTATGCGG AGCTGCATGA GATTGGGAGG GGACGATTCT CCATTGTAAA GAAATGCGTT 
CACAAAGCTA CCCGGAAAGA CGTGGCTGTA AAATTCATCA GTAAAAAAAT GAAGAAGAAG 
GAGCAAGCAG CCCACGAGGC AGCTCTGTTA CAGCACTTAC AGCATCCTCA GTACATCACT 
ATCCATGATA CCTATGAGTC TCCTACTTCT TACATCCTAG TCCTGGAGCT GATGGATGAT 
GGTCGTCTCC TAGATTATCT AATGAACCAT GATGAACTTA TGGAGGAAAA AGTAGCTTTC 
TACATAAGAG ACACAATGGA AGCCTTGCAG TATCTTCACA ATTGCCGAGT GGCTCATTTA 
GATATAAAGC CAGAGAATCT GCTCATCGAT TTAAGGATCC CAGTTCCTCG TGTCAAGATC 
ATTGATTTGG AAGATGCAGT TCAAATCACA GGCCATTACC ACGTCCACCA TCTCCTTGGA 
AACCCAGAGT TTGCAGCCCC TGAAGTTATC CAGGGCCTCC CCGTGTCCCT GAGCACAGAC 
ATCTGGAGCA TCGGGGTCCT CACCTACGTC ATGCTGAGCG GTGTCTCCCC GTTCCTGGAT 
GAAAGCAAAG AGGAGACGTG TATCAATGTG TGCAGAGTCG ATTTCAGCTT CCCGCCCGAG 
TACTTCTCTG ATGTGAGCCA TGCTGCTAGG GATTTCATCA ACGTCATCCT CCAGGAAGAC 
TCCAGGAGGA GGCCGACAGC AGCCACTTGT TTACAGCACC CATGGCTGCA GCCACACAAT 
GGCAGCTACT CCAAAATCCC GCTGGATACC TCCCGCTTGG CATCCTTCAT TGAGCGCCGC 
AGACACCAGT ACGACGTGCA CCCCGTCCCC GGTGTCAAGA GTTTCCTGAT GAGCAGGCTG 
AACCCCGGGA CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KALRN ( XM_015289985.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145471.1 Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X9, mRNA.

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CloneID	OGa38681
Clone ID Related Accession (Same CDS sequence)	XM_015289985.1 , XM_015289985.2
Accession Version	XM_015289985.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	kalirin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289985.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGCGG GCAGGCGCGG CTATCCAGCC CGCTCCGGTG TAGGATGGCT CTTGGCGAAG 
TGCTGCTGCT GCTCCTGCAA AGAGGCATAT TCAGTCTCTT CCAATGAAAA CGGGGGCAAG 
TCCGATTCAG TGGCCAACTT ACAGCCCCAG CCATCCCTCA ACTCCATCCA GAGCTCTCCC 
GGCCCCAAGC GCTCGACCAA CACGCTGAAG AAATGGCTGA CGAGCCCCGT GCGCCGGCTG 
AACAGCGGGA AGGCCGAGAG CAACATCAAG AAGCAAAAGA AGGTGCGAGA TGGCAGGAAG 
AGCTTTGACC TCGGCTCGCC GAAGACTGGA GATGAAACCA CTCCTCAAGG AGACAGCGCG 
GATGAGAAGA GCAAGAAGGG TTGGGGAGAA GATGAGCCAG ATGAAGAGTC TCACACACCT 
CTTCCTCCTC CTATGAAGAT TTTTGACAAT GATCCAACCC AAGATGAGAT GTCCTCTTCT 
TTGCTAGCTG CTCGGCAGAG CTCCTCCGAT GTCCCAACTG CTGCGGATCT TGTCAGTGCA 
ATAGAACAGT TGGTCAAAAG TAAGCTGACC CTTGAAGGAG GATCTTACCG AGGAAGCTTA 
AAAGATGCCA CAGGCTGCTT GAATGAAGGA ATGGCGCCTT CAACTCCCCC AAGAAACCTT 
GAAGAGGAAC AAAAAGCCAA AGCAATGAGA GGCAGGATGT TTGTCTTAAA TGAACTGGTC 
CAGACTGAAA AAGACTACGT CAAAGACTTG GGAACCGTTG TGGAGGGCTT CATGAAGAGA 
ATAGAAGAAA AGGGAGTTTC CGAAGATATG AAAGGGAAAG ACAAGATAGT GTTTGGCAAT 
ATTCACCAGA TCTATGACTG GCACAAAGAC TTTTTCCTGG CTGAACTGGA AAAATGTCTT 
CAGGAGCCTG ACCGTTTAGC ACAGCTTTTT ATTAAACATG AGCGGCGATT ACACATGTAT 
GTGGTGTACT GCCAAAACAA GCCACGATCT GAATATATAG TAGCTGAATA CGAGGCATAT 
TTTGAGGAGG TTCAACAGGA AATAAACCAA CGGCTGACCA TCAGTGACTT TCTGATCAAA 
CCCATTCAGA GAATAACTAA ATATCAGCTT CTTCTCAAGG ACTTCCTGAG GTACAGTGAA 
AAAGCTGGTC TGGAGTGCTC TGAAATAGAG AAAGCAGTTG AGTTAATGTG CCTTGTACCA 
AAACGTTGCA ATGATATGAT GAACCTGGGT CGCCTCCAAG GCTTTGAGGG TAAGCTGACA 
GCCCAGGGCA AGCTGCTGCA GCAGGACACC TTCTACGTGA CAGAGCATGA CTCCGGAGTT 
CAGTCCCGAC CAAAGGAACG TAGGGTGTTT CTCTTTGAGC AAATAGTTAT CTTCAGCGAG 
CTGCTTAGGA AAGGGTCTTT AACACCAGGC TACATGTTCA AGAGAAGCAT AAAGATGAAC 
TACCTTGTCA TTGAAGAAAA TGTGGATGGT GATCCCTGCA AGTTTGCTCT AATGAATCGG 
GAAACATCTG AGAAAGTTGT TTTACAGGCA GCTAATCCAG AAATTCAGCA GGCTTGGGTA 
CAGGACATCA ACCAGGTCCT GGACACACAA AGGGATTTCC TAAATGCACT GCAGTCTCCC 
ATAGAGTACC AACGCAAAGA AAGTAGCACA GCAGTGCTGA GGCCCCAGGC TGGTAGGGTC 
GCTCAGCCCA ACAGCAGACC CTATTCTTCT GTTCCAGTAG GGTCTGAGAA GCCTTTGAAG 
GCTACAAGCC GTAACCCATC TCTCCCACCC CTGAAGATAT CTACCTCCAA CGGCAGTACA 
GGGTATGAAC AGCCCGGAGA CAAGTATGAA CAAAGCAAGA CTGATTTGGG AGGGTGCAAT 
GGGACATCTT CCATGGTGGT GATTAAAGAT TACTATGCAG TGAAGGAGGA CGAGATCTGT 
GTGAATCAAG GAGAGGTGGT TCAGATCCTT GCTATCAACC AGCAAAACAT GTTCCTGGTG 
TACCAGCCTG CAAATGACCA CTCTCCAGCT GCTGAAGGAT GGATCCCTGG CAATATCTTG 
GCTCCCCTCA CTAAACCCAC TACAGATAAT AGCGACGGAA GCATCAAGAA GTCATGTTCA 
TGGCATACCC TGCGCATGAG AAAGCGGGCG GAAAAGGAGA GCAGTGGCAA AAATGAAGCC 
AATGGGCCAC GTAAATCCAA GGATATTCTG GGGAACAAAG TCTCTGTTAA AGAGACAAAC 
AGCTCAGAGG AATCAGAGTG TGATGACCTT GACCCCAATA CTAGCATGGA GATAATCAAC 
CCCAATTTCA TTCAGGAAGT GGCTCCAGAG TTCCTTGTGC TGTTGGTGGA TATCACCTGC 
CTGCTTGGCG ACACTGTGAT GCTGCAGTGC AAAGTCTGCG GCCGGCCCAA ACCAACAATA 
ACCTGGAAAG GACCTGATCA AAACATTCTT GACAATGACA ACAGCACGGC CACATACACG 
GTGTCATCCT GTGACTCTGG AGAGCTCATG CTGAAGATCT GCAATGTGAT GCCTCAGGAC 
AGTGGCATTT ACACTTGTGT GGCAACTAAC GAGCATGGAA CGGCATCAAC CTCAGCCACA 
ATCAAAGTGC AAGGTGTACC GGCTGCCCCA AATCGCCCCA TTGCTCAGGA GAGAAGCTGC 
ACCTCTGTGA TCCTTCGCTG GCTGCCTCCA TCCAGTACAG GCAATTGCAC CATTTCTGGC 
TACACAGTGG AGTACAGAGA AGAAGGCTCA CAGGTCTGGC AGCAGTCAGT GGCCTCCACA 
CTGGACACTT ACCTTGTCAT TGAAGACCTG TCCCCAGGCT GCCAGTATCA GTTCCGTGTG 
AGTGCCAGCA ACCCCTGGGG GATCAGCTTG CCCAGTGACC CATCTGAATT CGTGAGGCTT 
CCAGAGTATG ATTCTGCTGC TGATGGTGCC ACTATTTCGT GGAAGGAAAA CTTTGATCTC 
GCTTATGCGG AGCTGCATGA GATTGGGAGG GGACGATTCT CCATTGTAAA GAAATGCGTT 
CACAAAGCTA CCCGGAAAGA CGTGGCTGTA AAATTCATCA GTAAAAAAAT GAAGAAGAAG 
GAGCAAGCAG CCCACGAGGC AGCTCTGTTA CAGCACTTAC AGCATCCTCA GTACATCACT 
ATCCATGATA CCTATGAGTC TCCTACTTCT TACATCCTAG TCCTGGAGCT GATGGATGAT 
GGTCGTCTCC TAGATTATCT AATGAACCAT GATGAACTTA TGGAGGAAAA AGTAGCTTTC 
TACATAAGAG ACACAATGGA AGCCTTGCAG TATCTTCACA ATTGCCGAGT GGCTCATTTA 
GATATAAAGC CAGAGAATCT GCTCATCGAT TTAAGGATCC CAGTTCCTCG TGTCAAGATC 
ATTGATTTGG AAGATGCAGT TCAAATCACA GGCCATTACC ACGTCCACCA TCTCCTTGGA 
AACCCAGAGT TTGCAGCCCC TGAAGTTATC CAGGGCCTCC CCGTGTCCCT GAGCACAGAC 
ATCTGGAGCA TCGGGGTCCT CACCTACGTC ATGCTGAGCG GTGTCTCCCC GTTCCTGGAT 
GAAAGCAAAG AGGAGACGTG TATCAATGTG TGCAGAGTCG ATTTCAGCTT CCCGCCCGAG 
TACTTCTCTG ATGTGAGCCA TGCTGCTAGG GATTTCATCA ACGTCATCCT CCAGGAAGAC 
TCCAGGAGGA GGCCGACAGC AGCCACTTGT TTACAGCACC CATGGCTGCA GCCACACAAT 
GGCAGCTACT CCAAAATCCC GCTGGATACC TCCCGCTTGG CATCCTTCAT TGAGCGCCGC 
AGACACCAGT ACGACGTGCA CCCCGTCCCC GGTGTCAAGA GTTTCCTGAT GAGCAGGCTG 
AACCCCGGGA CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145471.1
CDS	188..4042
Translation	MKAGRRGYPARSGVGWLLAKCCCCSCKEAYSVSSNENGGKSDSVANLQPQPSLNSIQSSPGPKRSTNTLKKWLTSPVRRLNSGKAESNIKKQKKVRDGRKSFDLGSPKTGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSSLLAARQSSSDVPTAADLVSAIEQLVKSKLTLEGGSYRGSLKDATGCLNEGMAPSTPPRNLEEEQKAKAMRGRMFVLNELVQTEKDYVKDLGTVVEGFMKRIEEKGVSEDMKGKDKIVFGNIHQIYDWHKDFFLAELEKCLQEPDRLAQLFIKHERRLHMYVVYCQNKPRSEYIVAEYEAYFEEVQQEINQRLTISDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSEIEKAVELMCLVPKRCNDMMNLGRLQGFEGKLTAQGKLLQQDTFYVTEHDSGVQSRPKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVIEENVDGDPCKFALMNRETSEKVVLQAANPEIQQAWVQDINQVLDTQRDFLNALQSPIEYQRKESSTAVLRPQAGRVAQPNSRPYSSVPVGSEKPLKATSRNPSLPPLKISTSNGSTGYEQPGDKYEQSKTDLGGCNGTSSMVVIKDYYAVKEDEICVNQGEVVQILAINQQNMFLVYQPANDHSPAAEGWIPGNILAPLTKPTTDNSDGSIKKSCSWHTLRMRKRAEKESSGKNEANGPRKSKDILGNKVSVKETNSSEESECDDLDPNTSMEIINPNFIQEVAPEFLVLLVDITCLLGDTVMLQCKVCGRPKPTITWKGPDQNILDNDNSTATYTVSSCDSGELMLKICNVMPQDSGIYTCVATNEHGTASTSATIKVQGVPAAPNRPIAQERSCTSVILRWLPPSSTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCQYQFRVSASNPWGISLPSDPSEFVRLPEYDSAADGATISWKENFDLAYAELHEIGRGRFSIVKKCVHKATRKDVAVKFISKKMKKKEQAAHEAALLQHLQHPQYITIHDTYESPTSYILVLELMDDGRLLDYLMNHDELMEEKVAFYIRDTMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKIIDLEDAVQITGHYHVHHLLGNPEFAAPEVIQGLPVSLSTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPPEYFSDVSHAARDFINVILQEDSRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLASFIERRRHQYDVHPVPGVKSFLMSRLNPGT

Target ORF information:

RefSeq Version	XM_015289985.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289985.2

ORF Insert Sequence:

ATGAAAGCGG GCAGGCGCGG CTATCCAGCC CGCTCCGGTG TAGGATGGCT CTTGGCGAAG 
TGCTGCTGCT GCTCCTGCAA AGAGGCATAT TCAGTCTCTT CCAATGAAAA CGGGGGCAAG 
TCCGATTCAG TGGCCAACTT ACAGCCCCAG CCATCCCTCA ACTCCATCCA GAGCTCTCCC 
GGCCCCAAGC GCTCGACCAA CACGCTGAAG AAATGGCTGA CGAGCCCCGT GCGCCGGCTG 
AACAGCGGGA AGGCCGAGAG CAACATCAAG AAGCAAAAGA AGGTGCGAGA TGGCAGGAAG 
AGCTTTGACC TCGGCTCGCC GAAGACTGGA GATGAAACCA CTCCTCAAGG AGACAGCGCG 
GATGAGAAGA GCAAGAAGGG TTGGGGAGAA GATGAGCCAG ATGAAGAGTC TCACACACCT 
CTTCCTCCTC CTATGAAGAT TTTTGACAAT GATCCAACCC AAGATGAGAT GTCCTCTTCT 
TTGCTAGCTG CTCGGCAGAG CTCCTCCGAT GTCCCAACTG CTGCGGATCT TGTCAGTGCA 
ATAGAACAGT TGGTCAAAAG TAAGCTGACC CTTGAAGGAG GATCTTACCG AGGAAGCTTA 
AAAGATGCCA CAGGCTGCTT GAATGAAGGA ATGGCGCCTT CAACTCCCCC AAGAAACCTT 
GAAGAGGAAC AAAAAGCCAA AGCAATGAGA GGCAGGATGT TTGTCTTAAA TGAACTGGTC 
CAGACTGAAA AAGACTACGT CAAAGACTTG GGAACCGTTG TGGAGGGCTT CATGAAGAGA 
ATAGAAGAAA AGGGAGTTTC CGAAGATATG AAAGGGAAAG ACAAGATAGT GTTTGGCAAT 
ATTCACCAGA TCTATGACTG GCACAAAGAC TTTTTCCTGG CTGAACTGGA AAAATGTCTT 
CAGGAGCCTG ACCGTTTAGC ACAGCTTTTT ATTAAACATG AGCGGCGATT ACACATGTAT 
GTGGTGTACT GCCAAAACAA GCCACGATCT GAATATATAG TAGCTGAATA CGAGGCATAT 
TTTGAGGAGG TTCAACAGGA AATAAACCAA CGGCTGACCA TCAGTGACTT TCTGATCAAA 
CCCATTCAGA GAATAACTAA ATATCAGCTT CTTCTCAAGG ACTTCCTGAG GTACAGTGAA 
AAAGCTGGTC TGGAGTGCTC TGAAATAGAG AAAGCAGTTG AGTTAATGTG CCTTGTACCA 
AAACGTTGCA ATGATATGAT GAACCTGGGT CGCCTCCAAG GCTTTGAGGG TAAGCTGACA 
GCCCAGGGCA AGCTGCTGCA GCAGGACACC TTCTACGTGA CAGAGCATGA CTCCGGAGTT 
CAGTCCCGAC CAAAGGAACG TAGGGTGTTT CTCTTTGAGC AAATAGTTAT CTTCAGCGAG 
CTGCTTAGGA AAGGGTCTTT AACACCAGGC TACATGTTCA AGAGAAGCAT AAAGATGAAC 
TACCTTGTCA TTGAAGAAAA TGTGGATGGT GATCCCTGCA AGTTTGCTCT AATGAATCGG 
GAAACATCTG AGAAAGTTGT TTTACAGGCA GCTAATCCAG AAATTCAGCA GGCTTGGGTA 
CAGGACATCA ACCAGGTCCT GGACACACAA AGGGATTTCC TAAATGCACT GCAGTCTCCC 
ATAGAGTACC AACGCAAAGA AAGTAGCACA GCAGTGCTGA GGCCCCAGGC TGGTAGGGTC 
GCTCAGCCCA ACAGCAGACC CTATTCTTCT GTTCCAGTAG GGTCTGAGAA GCCTTTGAAG 
GCTACAAGCC GTAACCCATC TCTCCCACCC CTGAAGATAT CTACCTCCAA CGGCAGTACA 
GGGTATGAAC AGCCCGGAGA CAAGTATGAA CAAAGCAAGA CTGATTTGGG AGGGTGCAAT 
GGGACATCTT CCATGGTGGT GATTAAAGAT TACTATGCAG TGAAGGAGGA CGAGATCTGT 
GTGAATCAAG GAGAGGTGGT TCAGATCCTT GCTATCAACC AGCAAAACAT GTTCCTGGTG 
TACCAGCCTG CAAATGACCA CTCTCCAGCT GCTGAAGGAT GGATCCCTGG CAATATCTTG 
GCTCCCCTCA CTAAACCCAC TACAGATAAT AGCGACGGAA GCATCAAGAA GTCATGTTCA 
TGGCATACCC TGCGCATGAG AAAGCGGGCG GAAAAGGAGA GCAGTGGCAA AAATGAAGCC 
AATGGGCCAC GTAAATCCAA GGATATTCTG GGGAACAAAG TCTCTGTTAA AGAGACAAAC 
AGCTCAGAGG AATCAGAGTG TGATGACCTT GACCCCAATA CTAGCATGGA GATAATCAAC 
CCCAATTTCA TTCAGGAAGT GGCTCCAGAG TTCCTTGTGC TGTTGGTGGA TATCACCTGC 
CTGCTTGGCG ACACTGTGAT GCTGCAGTGC AAAGTCTGCG GCCGGCCCAA ACCAACAATA 
ACCTGGAAAG GACCTGATCA AAACATTCTT GACAATGACA ACAGCACGGC CACATACACG 
GTGTCATCCT GTGACTCTGG AGAGCTCATG CTGAAGATCT GCAATGTGAT GCCTCAGGAC 
AGTGGCATTT ACACTTGTGT GGCAACTAAC GAGCATGGAA CGGCATCAAC CTCAGCCACA 
ATCAAAGTGC AAGGTGTACC GGCTGCCCCA AATCGCCCCA TTGCTCAGGA GAGAAGCTGC 
ACCTCTGTGA TCCTTCGCTG GCTGCCTCCA TCCAGTACAG GCAATTGCAC CATTTCTGGC 
TACACAGTGG AGTACAGAGA AGAAGGCTCA CAGGTCTGGC AGCAGTCAGT GGCCTCCACA 
CTGGACACTT ACCTTGTCAT TGAAGACCTG TCCCCAGGCT GCCAGTATCA GTTCCGTGTG 
AGTGCCAGCA ACCCCTGGGG GATCAGCTTG CCCAGTGACC CATCTGAATT CGTGAGGCTT 
CCAGAGTATG ATTCTGCTGC TGATGGTGCC ACTATTTCGT GGAAGGAAAA CTTTGATCTC 
GCTTATGCGG AGCTGCATGA GATTGGGAGG GGACGATTCT CCATTGTAAA GAAATGCGTT 
CACAAAGCTA CCCGGAAAGA CGTGGCTGTA AAATTCATCA GTAAAAAAAT GAAGAAGAAG 
GAGCAAGCAG CCCACGAGGC AGCTCTGTTA CAGCACTTAC AGCATCCTCA GTACATCACT 
ATCCATGATA CCTATGAGTC TCCTACTTCT TACATCCTAG TCCTGGAGCT GATGGATGAT 
GGTCGTCTCC TAGATTATCT AATGAACCAT GATGAACTTA TGGAGGAAAA AGTAGCTTTC 
TACATAAGAG ACACAATGGA AGCCTTGCAG TATCTTCACA ATTGCCGAGT GGCTCATTTA 
GATATAAAGC CAGAGAATCT GCTCATCGAT TTAAGGATCC CAGTTCCTCG TGTCAAGATC 
ATTGATTTGG AAGATGCAGT TCAAATCACA GGCCATTACC ACGTCCACCA TCTCCTTGGA 
AACCCAGAGT TTGCAGCCCC TGAAGTTATC CAGGGCCTCC CCGTGTCCCT GAGCACAGAC 
ATCTGGAGCA TCGGGGTCCT CACCTACGTC ATGCTGAGCG GTGTCTCCCC GTTCCTGGAT 
GAAAGCAAAG AGGAGACGTG TATCAATGTG TGCAGAGTCG ATTTCAGCTT CCCGCCCGAG 
TACTTCTCTG ATGTGAGCCA TGCTGCTAGG GATTTCATCA ACGTCATCCT CCAGGAAGAC 
TCCAGGAGGA GGCCGACAGC AGCCACTTGT TTACAGCACC CATGGCTGCA GCCACACAAT 
GGCAGCTACT CCAAAATCCC GCTGGATACC TCCCGCTTGG CATCCTTCAT TGAGCGCCGC 
AGACACCAGT ACGACGTGCA CCCCGTCCCC GGTGTCAAGA GTTTCCTGAT GAGCAGGCTG 
AACCCCGGGA CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KALRN ( XM_015289984.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145470.1 Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X10, mRNA.

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CloneID	OGa38680
Clone ID Related Accession (Same CDS sequence)	XM_015289984.1 , XM_015289984.2
Accession Version	XM_015289984.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	kalirin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGCATT TCGTCTACGG CAGAGACACA 
GGACAGTTGT CTGGAGGGTG TGAACTAACC GTGGTGCTCC AGGACTTCAC GGCGGGCCAC 
AGCAGCGAGC TGAGCATTCA GGTGGGGCAG ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA 
AGGCCAGGCT GGTGCTTGGT GCGGACCACG GAGCGGAGCC CACCTCAGGA AGGCCTGGTC 
CCCAGCAGCG CTCTCTGCAT TTCCCATTCC CGCAGCAGCG TGGAGATGGA TTGCTTCTTC 
CCTTCAGGAA AAGGGGCTGT CAGAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145470.1
CDS	241..5430
Translation	MNPTEGAAEEGAVPDSEVDAFFRTGSFRNDGLKAADVLPILKEKVAFVSGGRDKRGGPVLTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLAKLVDPSQLTDEFEGSLDYNHDEWIELRVSLEEFFNSAIHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVIKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKELLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAVSNNKTPHNSSISHIESVLQQLDEAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDIDLATQVQELLEFLHEKQHELELNADQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEVLLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEIDHVIPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPGVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHNSTGESAEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKRYRDFSLRMGKYRYSLEKALGINTEDNKDLELDIIPASLSDREVKLRDANHEVNEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPAGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSSQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDSFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASSIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKQRNNSRRDGVDDADSQGDGSSQPDTISIASRTSQNTVDSDKHFVYGRDTGQLSGGCELTVVLQDFTAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPSGKGAVRA

Target ORF information:

RefSeq Version	XM_015289984.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289984.1

ORF Insert Sequence:

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGCATT TCGTCTACGG CAGAGACACA 
GGACAGTTGT CTGGAGGGTG TGAACTAACC GTGGTGCTCC AGGACTTCAC GGCGGGCCAC 
AGCAGCGAGC TGAGCATTCA GGTGGGGCAG ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA 
AGGCCAGGCT GGTGCTTGGT GCGGACCACG GAGCGGAGCC CACCTCAGGA AGGCCTGGTC 
CCCAGCAGCG CTCTCTGCAT TTCCCATTCC CGCAGCAGCG TGGAGATGGA TTGCTTCTTC 
CCTTCAGGAA AAGGGGCTGT CAGAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KALRN ( XM_015289984.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145470.1 Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X8, mRNA.

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CloneID	OGa38680
Clone ID Related Accession (Same CDS sequence)	XM_015289984.1 , XM_015289984.2
Accession Version	XM_015289984.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	kalirin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289984.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGCATT TCGTCTACGG CAGAGACACA 
GGACAGTTGT CTGGAGGGTG TGAACTAACC GTGGTGCTCC AGGACTTCAC GGCGGGCCAC 
AGCAGCGAGC TGAGCATTCA GGTGGGGCAG ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA 
AGGCCAGGCT GGTGCTTGGT GCGGACCACG GAGCGGAGCC CACCTCAGGA AGGCCTGGTC 
CCCAGCAGCG CTCTCTGCAT TTCCCATTCC CGCAGCAGCG TGGAGATGGA TTGCTTCTTC 
CCTTCAGGAA AAGGGGCTGT CAGAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145470.1
CDS	245..5434
Translation	MNPTEGAAEEGAVPDSEVDAFFRTGSFRNDGLKAADVLPILKEKVAFVSGGRDKRGGPVLTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLAKLVDPSQLTDEFEGSLDYNHDEWIELRVSLEEFFNSAIHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVIKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKELLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAVSNNKTPHNSSISHIESVLQQLDEAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDIDLATQVQELLEFLHEKQHELELNADQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEVLLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEIDHVIPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPGVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHNSTGESAEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKRYRDFSLRMGKYRYSLEKALGINTEDNKDLELDIIPASLSDREVKLRDANHEVNEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPAGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSSQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDSFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASSIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKQRNNSRRDGVDDADSQGDGSSQPDTISIASRTSQNTVDSDKHFVYGRDTGQLSGGCELTVVLQDFTAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPSGKGAVRA

Target ORF information:

RefSeq Version	XM_015289984.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289984.2

ORF Insert Sequence:

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGCATT TCGTCTACGG CAGAGACACA 
GGACAGTTGT CTGGAGGGTG TGAACTAACC GTGGTGCTCC AGGACTTCAC GGCGGGCCAC 
AGCAGCGAGC TGAGCATTCA GGTGGGGCAG ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA 
AGGCCAGGCT GGTGCTTGGT GCGGACCACG GAGCGGAGCC CACCTCAGGA AGGCCTGGTC 
CCCAGCAGCG CTCTCTGCAT TTCCCATTCC CGCAGCAGCG TGGAGATGGA TTGCTTCTTC 
CCTTCAGGAA AAGGGGCTGT CAGAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KALRN ( XM_015289983.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145469.1 Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OGa38679
Clone ID Related Accession (Same CDS sequence)	XM_015289983.1
Accession Version	XM_015289983.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	kalirin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATC TGCAGAAGGA GCTCTTAGAG GATGTCTGTG CTGACTCTGT GGATGCGGTT 
CAGGAGCTTA TCAAGCAGTT TCAGCAGCAG CAAACAGCCA CCTTGGATGC TACCCTCAAT 
GTTATTAAGG AAGGGGAAGA TCTAATCCAG CAGCTCAGGG ACTCCGCCGT TTCCAACAAT 
AAGACCCCAC ACAACAGCTC CATCAGCCAC ATTGAATCTG TACTTCAGCA GCTGGACGAG 
GCCCAGGTAC AGATGGAAGA GCTCTTCCAT GAGAGGAAGA TTAAGCTAGA TATCTTTCTT 
CAGCTCCGGA TCTTTGAGCA GTACACCATT GAGGTTACAG CAGAGTTAGA TGCCTGGAAT 
GAGGATCTGC TGAGACAAAT GAATGATTTC AATACCGAGG ACCTTACTCT GGCTGAGCAA 
CGTTTGCAGC GTCACACCGA GAGGAAGTTG GCCATGAACA ACATGACTTT TGAAGTCATC 
CAGCAAGGGC AAGATTTACA CCAATACATC ATGGAGGTCC AGGCTTCAGG CATCGAGCTG 
ATCTGTGAAA AGGACATTGA CCTTGCAACA CAGGTTCAAG AGCTGCTGGA ATTCCTTCAT 
GAGAAGCAGC ATGAGCTGGA GCTTAATGCT GACCAAACTC ACAAGAGGTT AGAGCAGTGC 
CTACAGCTCC GGCACCTACA GGCTGAGGTC AAGCAGGTGC TTGGTTGGAT CCGGAATGGT 
GAATCAATGC TGAATGCAAG CCTCGTCAAT GCAAGCTCCC TCTCTGAGGC TGAGCAGCTC 
CAGCGAGAGC ATGAGCAATT TCAGCTGGCC ATAGAGTCCC TCTTTCATGC CACTTCCTTA 
CAGAAGACAC ACCAGAGTGC CCTGCAGGTC CAGCAGAAAG CCGAAGTGCT GCTGCAGGCT 
GGGCATTATG ATGCTGATGC CATCAGAGAA TGCGCTGAAA AGGTGGCCCT GCACTGGCAG 
CAGCTGATGC TGAAGATGGA GGACAGGCTC AAGCTGGTCA ATGCCTCGGT GGCCTTCTAC 
AAAACCTCTG AGCAGGTGTG CAGTGTATTA GAGAGCCTGG AGCAAGAATA CAGACGTGAT 
GAAGACTGGT GTGGAGGTCG AGATAAACTT GGACCAGCAG CTGAGATAGA CCACGTCATC 
CCTCTGATCA GCAAGCATCT GGAGCAGAAG GAGGCTTTTC TCAAGGCCTG CACACTGGCT 
CGAAGGAATG CTGAGGTATT CCTCAAGTAC ATCCACAGGA ACAATGTCAG CATGCCCGGA 
GTCGCAAGCC ATACACGGGG ACCTGAGCAG CAAGTGAAAG CCATCCTGAG TGAGTTGCTG 
CAGAGGGAGA ACCGGGTCCT TCACTTCTGG ACCCTGAAGA AGCGGAGGTT AGATCAGTGC 
CAGCAGTACG TTGTATTTGA GCGCAGTGCC AAGCAGGCCC TGGACTGGAT CCAAGAGACA 
GGCGAATATT ACCTCTCTAC TCACAACTCT ACAGGGGAAT CAGCAGAAGA AACTCAGGAA 
CTCCTGAAGG AATATGGGGA ATTCAGAGTA CCTGCGAAGC AAACAAAGGA GAAAGTGAAG 
CTCCTCATCC AGCTGGCTGA CAGCTTTGTA GAAAAAGGAC ATATACACGC CACTGAAATC 
AGGAAGTGGG TCACCACCGT TGACAAACGC TACCGTGACT TCTCTCTCAG GATGGGGAAA 
TACCGCTACT CCCTGGAGAA AGCCCTTGGA ATCAATACAG AGGATAACAA GGACCTCGAA 
CTAGATATTA TTCCAGCAAG TCTTTCAGAC CGGGAGGTAA AGCTGCGAGA TGCAAATCAC 
GAAGTCAATG AAGAAAAAAG AAAGTCTGCT AGAAAGAAAG AATTCATTAT GGCCGAGCTT 
CTTCAGACAG AGAAAGCTTA TGTCAGGGAC TTACATGAAT GTTTAGAGAC TTACCTTTGG 
GAAATGACAA GTGGGGTGGA AGAAATACCC GCTGGGATCC TTAATAAAGA ACACATCATC 
TTTGGCAATA TTCAGGAGAT ATATGACTTC CATAACAACA TTTTCCTGAA AGAACTCGAG 
AAGTATGAGC AACTGCCTGA GGATGTGGGT CACTGCTTTG TCACCTGGGC AGATAAATTT 
CAGATGTATG TCACCTACTG CAAAAACAAG CCTGACTCCA GCCAGCTCAT CCTGGAACAC 
GCAGGGACTT TCTTTGATGA AATTCAGCAA AGGCATGGTC TGGCCAACTC TATCTCTTCT 
TATTTAATCA AGCCTGTCCA GAGGATCACA AAATATCAAC TCCTCCTGAA GGAACTGCTT 
ACCTGCTGTG AGGAAGGCAA AGGGGAACTC AAGGATGGTC TGGAAGTGAT GCTCAGTGTC 
CCCAAGAAAG CCAATGATGC AATGCACGTC AGCATGCTGG AAGGGTTTGA TGAGAATCTA 
GACGTCCAGG GAGAGCTGAT TCTTCAGGAT TCCTTCCAAG TGTGGGACCC CAAGTCTCTC 
ATTCGGAAAG GCCGTGAGAG ACATTTGTTT CTCTTTGAAA TCTCTTTGGT GTTTAGCAAA 
GAGATCAAAG ACTCTTCAGG ACACACAAAA TATGTTTACA AGAACAAGTT ACTGACCTCA 
GAACTGGGAG TGACTGAACA TGTTGAAGGA GATCCCTGTA AATTTGCCTT GTGGTCTGGA 
CGAACACCAT CCTCAGACAA TAAAACAGTG CTGAAAGCAT CGAGTATTGA AACAAAACAG 
GAGTGGATCA AAAATATCCG AGAAGTCATC CAAGAAAGAA TTATCCATCT GAAAGGAGCT 
CTGAAAGAGC CAATTCAGCT CCCCAAGACA CCAGCAAAGC AGCGAAACAA CAGTAGAAGA 
GATGGAGTAG ATGATGCAGA CAGCCAAGGA GATGGAAGCA GTCAACCTGA CACCATTTCC 
ATTGCATCCA GGACATCACA GAATACAGTG GACAGTGATA AGCATTTCGT CTACGGCAGA 
GACACAGGAC AGTTGTCTGG AGGGTGTGAA CTAACCGTGG TGCTCCAGGA CTTCACGGCG 
GGCCACAGCA GCGAGCTGAG CATTCAGGTG GGGCAGACAG TGGAGCTGCT GGAGAGGCCA 
AGTGAAAGGC CAGGCTGGTG CTTGGTGCGG ACCACGGAGC GGAGCCCACC TCAGGAAGGC 
CTGGTCCCCA GCAGCGCTCT CTGCATTTCC CATTCCCGCA GCAGCGTGGA GATGGATTGC 
TTCTTCCCTT CAGGAAAAGA GGCATATTCA GTCTCTTCCA ATGAAAACGG GGGCAAGTCC 
GATTCAGTGG CCAACTTACA GCCCCAGCCA TCCCTCAACT CCATCCAGAG CTCTCCCGGC 
CCCAAGCGCT CGACCAACAC GCTGAAGAAA TGGCTGACGA GCCCCGTGCG CCGGCTGAAC 
AGCGGGAAGG CCGAGAGCAA CATCAAGAAG CAAAAGAAGG TGCGAGATGG CAGGAAGAGC 
TTTGACCTCG GCTCGCCGAA GACTGGAGAT GAAACCACTC CTCAAGGAGA CAGCGCGGAT 
GAGAAGAGCA AGAAGGGTTG GGGAGAAGAT GAGCCAGATG AAGAGTCTCA CACACCTCTT 
CCTCCTCCTA TGAAGATTTT TGACAATGAT CCAACCCAAG ATGAGATGTC CTCTTCTTTG 
CTAGCTGCTC GGCAGAGCTC CTCCGATGTC CCAACTGCTG CGGATCTTGT CAGTGCAATA 
GAACAGTTGG TCAAAAGTAA GCTGACCCTT GAAGGAGGAT CTTACCGAGG AAGCTTAAAA 
GATGCCACAG GCTGCTTGAA TGAAGGAATG GCGCCTTCAA CTCCCCCAAG AAACCTTGAA 
GAGGAACAAA AAGCCAAAGC AATGAGAGGC AGGATGTTTG TCTTAAATGA ACTGGTCCAG 
ACTGAAAAAG ACTACGTCAA AGACTTGGGA ACCGTTGTGG AGGGCTTCAT GAAGAGAATA 
GAAGAAAAGG GAGTTTCCGA AGATATGAAA GGGAAAGACA AGATAGTGTT TGGCAATATT 
CACCAGATCT ATGACTGGCA CAAAGACTTT TTCCTGGCTG AACTGGAAAA ATGTCTTCAG 
GAGCCTGACC GTTTAGCACA GCTTTTTATT AAACATGAGC GGCGATTACA CATGTATGTG 
GTGTACTGCC AAAACAAGCC ACGATCTGAA TATATAGTAG CTGAATACGA GGCATATTTT 
GAGGAGGTTC AACAGGAAAT AAACCAACGG CTGACCATCA GTGACTTTCT GATCAAACCC 
ATTCAGAGAA TAACTAAATA TCAGCTTCTT CTCAAGGACT TCCTGAGGTA CAGTGAAAAA 
GCTGGTCTGG AGTGCTCTGA AATAGAGAAA GCAGTTGAGT TAATGTGCCT TGTACCAAAA 
CGTTGCAATG ATATGATGAA CCTGGGTCGC CTCCAAGGCT TTGAGGGTAA GCTGACAGCC 
CAGGGCAAGC TGCTGCAGCA GGACACCTTC TACGTGACAG AGCATGACTC CGGAGTTCAG 
TCCCGACCAA AGGAACGTAG GGTGTTTCTC TTTGAGCAAA TAGTTATCTT CAGCGAGCTG 
CTTAGGAAAG GGTCTTTAAC ACCAGGCTAC ATGTTCAAGA GAAGCATAAA GATGAACTAC 
CTTGTCATTG AAGAAAATGT GGATGGTGAT CCCTGCAAGT TTGCTCTAAT GAATCGGGAA 
ACATCTGAGA AAGTTGTTTT ACAGGCAGCT AATCCAGAAA TTCAGCAGGC TTGGGTACAG 
GACATCAACC AGGTCCTGGA CACACAAAGG GATTTCCTAA ATGCACTGCA GTCTCCCATA 
GAGTACCAAC GCAAAGAAAG TAGCACAGCA GTGCTGAGGC CCCAGGCTGG TAGGGTCGCT 
CAGCCCAACA GCAGACCCTA TTCTTCTGTT CCAGTAGGGT CTGAGAAGCC TTTGAAGGCT 
ACAAGCCGTA ACCCATCTCT CCCACCCCTG AAGATATCTA CCTCCAACGG CAGTACAGGG 
TATGAACAGC CCGGAGACAA GTATGAACAA AGCAAGACTG ATTTGGGAGG GTGCAATGGG 
ACATCTTCCA TGGTGGTGAT TAAAGATTAC TATGCAGTGA AGGAGGACGA GATCTGTGTG 
AATCAAGGAG AGGTGGTTCA GATCCTTGCT ATCAACCAGC AAAACATGTT CCTGGTGTAC 
CAGCCTGCAA ATGACCACTC TCCAGCTGCT GAAGGATGGA TCCCTGGCAA TATCTTGGCT 
CCCCTCACTA AACCCACTAC AGATAATAGC GACGGAAGCA TCAAGAAGTC ATGTTCATGG 
CATACCCTGC GCATGAGAAA GCGGGCGGAA AAGGAGAGCA GTGGCAAAAA TGAAGCCAAT 
GGGCCACGTA AATCCAAGGA TATTCTGGGG AACAAAGTCT CTGTTAAAGA GACAAACAGC 
TCAGAGGAAT CAGAGTGTGA TGACCTTGAC CCCAATACTA GCATGGAGAT AATCAACCCC 
AATTTCATTC AGGAAGTGGC TCCAGAGTTC CTTGTGCTGT TGGTGGATAT CACCTGCCTG 
CTTGGCGACA CTGTGATGCT GCAGTGCAAA GTCTGCGGCC GGCCCAAACC AACAATAACC 
TGGAAAGGAC CTGATCAAAA CATTCTTGAC AATGACAACA GCACGGCCAC ATACACGGTG 
TCATCCTGTG ACTCTGGAGA GCTCATGCTG AAGATCTGCA ATGTGATGCC TCAGGACAGT 
GGCATTTACA CTTGTGTGGC AACTAACGAG CATGGAACGG CATCAACCTC AGCCACAATC 
AAAGTGCAAG GTGTACCGGC TGCCCCAAAT CGCCCCATTG CTCAGGAGAG AAGCTGCACC 
TCTGTGATCC TTCGCTGGCT GCCTCCATCC AGTACAGGCA ATTGCACCAT TTCTGGCTAC 
ACAGTGGAGT ACAGAGAAGA AGGCTCACAG GTCTGGCAGC AGTCAGTGGC CTCCACACTG 
GACACTTACC TTGTCATTGA AGACCTGTCC CCAGGCTGCC AGTATCAGTT CCGTGTGAGT 
GCCAGCAACC CCTGGGGGAT CAGCTTGCCC AGTGACCCAT CTGAATTCGT GAGGCTTCCA 
GAGTATGATT CTGCTGCTGA TGGTGCCACT ATTTCGTGGA AGGAAAACTT TGATCTCGCT 
TATGCGGAGC TGCATGAGAT TGGGAGGGGA CGATTCTCCA TTGTAAAGAA ATGCGTTCAC 
AAAGCTACCC GGAAAGACGT GGCTGTAAAA TTCATCAGTA AAAAAATGAA GAAGAAGGAG 
CAAGCAGCCC ACGAGGCAGC TCTGTTACAG CACTTACAGC ATCCTCAGTA CATCACTATC 
CATGATACCT ATGAGTCTCC TACTTCTTAC ATCCTAGTCC TGGAGCTGAT GGATGATGGT 
CGTCTCCTAG ATTATCTAAT GAACCATGAT GAACTTATGG AGGAAAAAGT AGCTTTCTAC 
ATAAGAGACA CAATGGAAGC CTTGCAGTAT CTTCACAATT GCCGAGTGGC TCATTTAGAT 
ATAAAGCCAG AGAATCTGCT CATCGATTTA AGGATCCCAG TTCCTCGTGT CAAGATCATT 
GATTTGGAAG ATGCAGTTCA AATCACAGGC CATTACCACG TCCACCATCT CCTTGGAAAC 
CCAGAGTTTG CAGCCCCTGA AGTTATCCAG GGCCTCCCCG TGTCCCTGAG CACAGACATC 
TGGAGCATCG GGGTCCTCAC CTACGTCATG CTGAGCGGTG TCTCCCCGTT CCTGGATGAA 
AGCAAAGAGG AGACGTGTAT CAATGTGTGC AGAGTCGATT TCAGCTTCCC GCCCGAGTAC 
TTCTCTGATG TGAGCCATGC TGCTAGGGAT TTCATCAACG TCATCCTCCA GGAAGACTCC 
AGGAGGAGGC CGACAGCAGC CACTTGTTTA CAGCACCCAT GGCTGCAGCC ACACAATGGC 
AGCTACTCCA AAATCCCGCT GGATACCTCC CGCTTGGCAT CCTTCATTGA GCGCCGCAGA 
CACCAGTACG ACGTGCACCC CGTCCCCGGT GTCAAGAGTT TCCTGATGAG CAGGCTGAAC 
CCCGGGACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145469.1
CDS	36..7007
Translation	MEDLQKELLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAVSNNKTPHNSSISHIESVLQQLDEAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDIDLATQVQELLEFLHEKQHELELNADQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEVLLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEIDHVIPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPGVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHNSTGESAEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKRYRDFSLRMGKYRYSLEKALGINTEDNKDLELDIIPASLSDREVKLRDANHEVNEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPAGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSSQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDSFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASSIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKQRNNSRRDGVDDADSQGDGSSQPDTISIASRTSQNTVDSDKHFVYGRDTGQLSGGCELTVVLQDFTAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPSGKEAYSVSSNENGGKSDSVANLQPQPSLNSIQSSPGPKRSTNTLKKWLTSPVRRLNSGKAESNIKKQKKVRDGRKSFDLGSPKTGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSSLLAARQSSSDVPTAADLVSAIEQLVKSKLTLEGGSYRGSLKDATGCLNEGMAPSTPPRNLEEEQKAKAMRGRMFVLNELVQTEKDYVKDLGTVVEGFMKRIEEKGVSEDMKGKDKIVFGNIHQIYDWHKDFFLAELEKCLQEPDRLAQLFIKHERRLHMYVVYCQNKPRSEYIVAEYEAYFEEVQQEINQRLTISDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSEIEKAVELMCLVPKRCNDMMNLGRLQGFEGKLTAQGKLLQQDTFYVTEHDSGVQSRPKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVIEENVDGDPCKFALMNRETSEKVVLQAANPEIQQAWVQDINQVLDTQRDFLNALQSPIEYQRKESSTAVLRPQAGRVAQPNSRPYSSVPVGSEKPLKATSRNPSLPPLKISTSNGSTGYEQPGDKYEQSKTDLGGCNGTSSMVVIKDYYAVKEDEICVNQGEVVQILAINQQNMFLVYQPANDHSPAAEGWIPGNILAPLTKPTTDNSDGSIKKSCSWHTLRMRKRAEKESSGKNEANGPRKSKDILGNKVSVKETNSSEESECDDLDPNTSMEIINPNFIQEVAPEFLVLLVDITCLLGDTVMLQCKVCGRPKPTITWKGPDQNILDNDNSTATYTVSSCDSGELMLKICNVMPQDSGIYTCVATNEHGTASTSATIKVQGVPAAPNRPIAQERSCTSVILRWLPPSSTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCQYQFRVSASNPWGISLPSDPSEFVRLPEYDSAADGATISWKENFDLAYAELHEIGRGRFSIVKKCVHKATRKDVAVKFISKKMKKKEQAAHEAALLQHLQHPQYITIHDTYESPTSYILVLELMDDGRLLDYLMNHDELMEEKVAFYIRDTMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKIIDLEDAVQITGHYHVHHLLGNPEFAAPEVIQGLPVSLSTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPPEYFSDVSHAARDFINVILQEDSRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLASFIERRRHQYDVHPVPGVKSFLMSRLNPGT

Target ORF information:

RefSeq Version	XM_015289983.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289983.1

ORF Insert Sequence:

ATGGAAGATC TGCAGAAGGA GCTCTTAGAG GATGTCTGTG CTGACTCTGT GGATGCGGTT 
CAGGAGCTTA TCAAGCAGTT TCAGCAGCAG CAAACAGCCA CCTTGGATGC TACCCTCAAT 
GTTATTAAGG AAGGGGAAGA TCTAATCCAG CAGCTCAGGG ACTCCGCCGT TTCCAACAAT 
AAGACCCCAC ACAACAGCTC CATCAGCCAC ATTGAATCTG TACTTCAGCA GCTGGACGAG 
GCCCAGGTAC AGATGGAAGA GCTCTTCCAT GAGAGGAAGA TTAAGCTAGA TATCTTTCTT 
CAGCTCCGGA TCTTTGAGCA GTACACCATT GAGGTTACAG CAGAGTTAGA TGCCTGGAAT 
GAGGATCTGC TGAGACAAAT GAATGATTTC AATACCGAGG ACCTTACTCT GGCTGAGCAA 
CGTTTGCAGC GTCACACCGA GAGGAAGTTG GCCATGAACA ACATGACTTT TGAAGTCATC 
CAGCAAGGGC AAGATTTACA CCAATACATC ATGGAGGTCC AGGCTTCAGG CATCGAGCTG 
ATCTGTGAAA AGGACATTGA CCTTGCAACA CAGGTTCAAG AGCTGCTGGA ATTCCTTCAT 
GAGAAGCAGC ATGAGCTGGA GCTTAATGCT GACCAAACTC ACAAGAGGTT AGAGCAGTGC 
CTACAGCTCC GGCACCTACA GGCTGAGGTC AAGCAGGTGC TTGGTTGGAT CCGGAATGGT 
GAATCAATGC TGAATGCAAG CCTCGTCAAT GCAAGCTCCC TCTCTGAGGC TGAGCAGCTC 
CAGCGAGAGC ATGAGCAATT TCAGCTGGCC ATAGAGTCCC TCTTTCATGC CACTTCCTTA 
CAGAAGACAC ACCAGAGTGC CCTGCAGGTC CAGCAGAAAG CCGAAGTGCT GCTGCAGGCT 
GGGCATTATG ATGCTGATGC CATCAGAGAA TGCGCTGAAA AGGTGGCCCT GCACTGGCAG 
CAGCTGATGC TGAAGATGGA GGACAGGCTC AAGCTGGTCA ATGCCTCGGT GGCCTTCTAC 
AAAACCTCTG AGCAGGTGTG CAGTGTATTA GAGAGCCTGG AGCAAGAATA CAGACGTGAT 
GAAGACTGGT GTGGAGGTCG AGATAAACTT GGACCAGCAG CTGAGATAGA CCACGTCATC 
CCTCTGATCA GCAAGCATCT GGAGCAGAAG GAGGCTTTTC TCAAGGCCTG CACACTGGCT 
CGAAGGAATG CTGAGGTATT CCTCAAGTAC ATCCACAGGA ACAATGTCAG CATGCCCGGA 
GTCGCAAGCC ATACACGGGG ACCTGAGCAG CAAGTGAAAG CCATCCTGAG TGAGTTGCTG 
CAGAGGGAGA ACCGGGTCCT TCACTTCTGG ACCCTGAAGA AGCGGAGGTT AGATCAGTGC 
CAGCAGTACG TTGTATTTGA GCGCAGTGCC AAGCAGGCCC TGGACTGGAT CCAAGAGACA 
GGCGAATATT ACCTCTCTAC TCACAACTCT ACAGGGGAAT CAGCAGAAGA AACTCAGGAA 
CTCCTGAAGG AATATGGGGA ATTCAGAGTA CCTGCGAAGC AAACAAAGGA GAAAGTGAAG 
CTCCTCATCC AGCTGGCTGA CAGCTTTGTA GAAAAAGGAC ATATACACGC CACTGAAATC 
AGGAAGTGGG TCACCACCGT TGACAAACGC TACCGTGACT TCTCTCTCAG GATGGGGAAA 
TACCGCTACT CCCTGGAGAA AGCCCTTGGA ATCAATACAG AGGATAACAA GGACCTCGAA 
CTAGATATTA TTCCAGCAAG TCTTTCAGAC CGGGAGGTAA AGCTGCGAGA TGCAAATCAC 
GAAGTCAATG AAGAAAAAAG AAAGTCTGCT AGAAAGAAAG AATTCATTAT GGCCGAGCTT 
CTTCAGACAG AGAAAGCTTA TGTCAGGGAC TTACATGAAT GTTTAGAGAC TTACCTTTGG 
GAAATGACAA GTGGGGTGGA AGAAATACCC GCTGGGATCC TTAATAAAGA ACACATCATC 
TTTGGCAATA TTCAGGAGAT ATATGACTTC CATAACAACA TTTTCCTGAA AGAACTCGAG 
AAGTATGAGC AACTGCCTGA GGATGTGGGT CACTGCTTTG TCACCTGGGC AGATAAATTT 
CAGATGTATG TCACCTACTG CAAAAACAAG CCTGACTCCA GCCAGCTCAT CCTGGAACAC 
GCAGGGACTT TCTTTGATGA AATTCAGCAA AGGCATGGTC TGGCCAACTC TATCTCTTCT 
TATTTAATCA AGCCTGTCCA GAGGATCACA AAATATCAAC TCCTCCTGAA GGAACTGCTT 
ACCTGCTGTG AGGAAGGCAA AGGGGAACTC AAGGATGGTC TGGAAGTGAT GCTCAGTGTC 
CCCAAGAAAG CCAATGATGC AATGCACGTC AGCATGCTGG AAGGGTTTGA TGAGAATCTA 
GACGTCCAGG GAGAGCTGAT TCTTCAGGAT TCCTTCCAAG TGTGGGACCC CAAGTCTCTC 
ATTCGGAAAG GCCGTGAGAG ACATTTGTTT CTCTTTGAAA TCTCTTTGGT GTTTAGCAAA 
GAGATCAAAG ACTCTTCAGG ACACACAAAA TATGTTTACA AGAACAAGTT ACTGACCTCA 
GAACTGGGAG TGACTGAACA TGTTGAAGGA GATCCCTGTA AATTTGCCTT GTGGTCTGGA 
CGAACACCAT CCTCAGACAA TAAAACAGTG CTGAAAGCAT CGAGTATTGA AACAAAACAG 
GAGTGGATCA AAAATATCCG AGAAGTCATC CAAGAAAGAA TTATCCATCT GAAAGGAGCT 
CTGAAAGAGC CAATTCAGCT CCCCAAGACA CCAGCAAAGC AGCGAAACAA CAGTAGAAGA 
GATGGAGTAG ATGATGCAGA CAGCCAAGGA GATGGAAGCA GTCAACCTGA CACCATTTCC 
ATTGCATCCA GGACATCACA GAATACAGTG GACAGTGATA AGCATTTCGT CTACGGCAGA 
GACACAGGAC AGTTGTCTGG AGGGTGTGAA CTAACCGTGG TGCTCCAGGA CTTCACGGCG 
GGCCACAGCA GCGAGCTGAG CATTCAGGTG GGGCAGACAG TGGAGCTGCT GGAGAGGCCA 
AGTGAAAGGC CAGGCTGGTG CTTGGTGCGG ACCACGGAGC GGAGCCCACC TCAGGAAGGC 
CTGGTCCCCA GCAGCGCTCT CTGCATTTCC CATTCCCGCA GCAGCGTGGA GATGGATTGC 
TTCTTCCCTT CAGGAAAAGA GGCATATTCA GTCTCTTCCA ATGAAAACGG GGGCAAGTCC 
GATTCAGTGG CCAACTTACA GCCCCAGCCA TCCCTCAACT CCATCCAGAG CTCTCCCGGC 
CCCAAGCGCT CGACCAACAC GCTGAAGAAA TGGCTGACGA GCCCCGTGCG CCGGCTGAAC 
AGCGGGAAGG CCGAGAGCAA CATCAAGAAG CAAAAGAAGG TGCGAGATGG CAGGAAGAGC 
TTTGACCTCG GCTCGCCGAA GACTGGAGAT GAAACCACTC CTCAAGGAGA CAGCGCGGAT 
GAGAAGAGCA AGAAGGGTTG GGGAGAAGAT GAGCCAGATG AAGAGTCTCA CACACCTCTT 
CCTCCTCCTA TGAAGATTTT TGACAATGAT CCAACCCAAG ATGAGATGTC CTCTTCTTTG 
CTAGCTGCTC GGCAGAGCTC CTCCGATGTC CCAACTGCTG CGGATCTTGT CAGTGCAATA 
GAACAGTTGG TCAAAAGTAA GCTGACCCTT GAAGGAGGAT CTTACCGAGG AAGCTTAAAA 
GATGCCACAG GCTGCTTGAA TGAAGGAATG GCGCCTTCAA CTCCCCCAAG AAACCTTGAA 
GAGGAACAAA AAGCCAAAGC AATGAGAGGC AGGATGTTTG TCTTAAATGA ACTGGTCCAG 
ACTGAAAAAG ACTACGTCAA AGACTTGGGA ACCGTTGTGG AGGGCTTCAT GAAGAGAATA 
GAAGAAAAGG GAGTTTCCGA AGATATGAAA GGGAAAGACA AGATAGTGTT TGGCAATATT 
CACCAGATCT ATGACTGGCA CAAAGACTTT TTCCTGGCTG AACTGGAAAA ATGTCTTCAG 
GAGCCTGACC GTTTAGCACA GCTTTTTATT AAACATGAGC GGCGATTACA CATGTATGTG 
GTGTACTGCC AAAACAAGCC ACGATCTGAA TATATAGTAG CTGAATACGA GGCATATTTT 
GAGGAGGTTC AACAGGAAAT AAACCAACGG CTGACCATCA GTGACTTTCT GATCAAACCC 
ATTCAGAGAA TAACTAAATA TCAGCTTCTT CTCAAGGACT TCCTGAGGTA CAGTGAAAAA 
GCTGGTCTGG AGTGCTCTGA AATAGAGAAA GCAGTTGAGT TAATGTGCCT TGTACCAAAA 
CGTTGCAATG ATATGATGAA CCTGGGTCGC CTCCAAGGCT TTGAGGGTAA GCTGACAGCC 
CAGGGCAAGC TGCTGCAGCA GGACACCTTC TACGTGACAG AGCATGACTC CGGAGTTCAG 
TCCCGACCAA AGGAACGTAG GGTGTTTCTC TTTGAGCAAA TAGTTATCTT CAGCGAGCTG 
CTTAGGAAAG GGTCTTTAAC ACCAGGCTAC ATGTTCAAGA GAAGCATAAA GATGAACTAC 
CTTGTCATTG AAGAAAATGT GGATGGTGAT CCCTGCAAGT TTGCTCTAAT GAATCGGGAA 
ACATCTGAGA AAGTTGTTTT ACAGGCAGCT AATCCAGAAA TTCAGCAGGC TTGGGTACAG 
GACATCAACC AGGTCCTGGA CACACAAAGG GATTTCCTAA ATGCACTGCA GTCTCCCATA 
GAGTACCAAC GCAAAGAAAG TAGCACAGCA GTGCTGAGGC CCCAGGCTGG TAGGGTCGCT 
CAGCCCAACA GCAGACCCTA TTCTTCTGTT CCAGTAGGGT CTGAGAAGCC TTTGAAGGCT 
ACAAGCCGTA ACCCATCTCT CCCACCCCTG AAGATATCTA CCTCCAACGG CAGTACAGGG 
TATGAACAGC CCGGAGACAA GTATGAACAA AGCAAGACTG ATTTGGGAGG GTGCAATGGG 
ACATCTTCCA TGGTGGTGAT TAAAGATTAC TATGCAGTGA AGGAGGACGA GATCTGTGTG 
AATCAAGGAG AGGTGGTTCA GATCCTTGCT ATCAACCAGC AAAACATGTT CCTGGTGTAC 
CAGCCTGCAA ATGACCACTC TCCAGCTGCT GAAGGATGGA TCCCTGGCAA TATCTTGGCT 
CCCCTCACTA AACCCACTAC AGATAATAGC GACGGAAGCA TCAAGAAGTC ATGTTCATGG 
CATACCCTGC GCATGAGAAA GCGGGCGGAA AAGGAGAGCA GTGGCAAAAA TGAAGCCAAT 
GGGCCACGTA AATCCAAGGA TATTCTGGGG AACAAAGTCT CTGTTAAAGA GACAAACAGC 
TCAGAGGAAT CAGAGTGTGA TGACCTTGAC CCCAATACTA GCATGGAGAT AATCAACCCC 
AATTTCATTC AGGAAGTGGC TCCAGAGTTC CTTGTGCTGT TGGTGGATAT CACCTGCCTG 
CTTGGCGACA CTGTGATGCT GCAGTGCAAA GTCTGCGGCC GGCCCAAACC AACAATAACC 
TGGAAAGGAC CTGATCAAAA CATTCTTGAC AATGACAACA GCACGGCCAC ATACACGGTG 
TCATCCTGTG ACTCTGGAGA GCTCATGCTG AAGATCTGCA ATGTGATGCC TCAGGACAGT 
GGCATTTACA CTTGTGTGGC AACTAACGAG CATGGAACGG CATCAACCTC AGCCACAATC 
AAAGTGCAAG GTGTACCGGC TGCCCCAAAT CGCCCCATTG CTCAGGAGAG AAGCTGCACC 
TCTGTGATCC TTCGCTGGCT GCCTCCATCC AGTACAGGCA ATTGCACCAT TTCTGGCTAC 
ACAGTGGAGT ACAGAGAAGA AGGCTCACAG GTCTGGCAGC AGTCAGTGGC CTCCACACTG 
GACACTTACC TTGTCATTGA AGACCTGTCC CCAGGCTGCC AGTATCAGTT CCGTGTGAGT 
GCCAGCAACC CCTGGGGGAT CAGCTTGCCC AGTGACCCAT CTGAATTCGT GAGGCTTCCA 
GAGTATGATT CTGCTGCTGA TGGTGCCACT ATTTCGTGGA AGGAAAACTT TGATCTCGCT 
TATGCGGAGC TGCATGAGAT TGGGAGGGGA CGATTCTCCA TTGTAAAGAA ATGCGTTCAC 
AAAGCTACCC GGAAAGACGT GGCTGTAAAA TTCATCAGTA AAAAAATGAA GAAGAAGGAG 
CAAGCAGCCC ACGAGGCAGC TCTGTTACAG CACTTACAGC ATCCTCAGTA CATCACTATC 
CATGATACCT ATGAGTCTCC TACTTCTTAC ATCCTAGTCC TGGAGCTGAT GGATGATGGT 
CGTCTCCTAG ATTATCTAAT GAACCATGAT GAACTTATGG AGGAAAAAGT AGCTTTCTAC 
ATAAGAGACA CAATGGAAGC CTTGCAGTAT CTTCACAATT GCCGAGTGGC TCATTTAGAT 
ATAAAGCCAG AGAATCTGCT CATCGATTTA AGGATCCCAG TTCCTCGTGT CAAGATCATT 
GATTTGGAAG ATGCAGTTCA AATCACAGGC CATTACCACG TCCACCATCT CCTTGGAAAC 
CCAGAGTTTG CAGCCCCTGA AGTTATCCAG GGCCTCCCCG TGTCCCTGAG CACAGACATC 
TGGAGCATCG GGGTCCTCAC CTACGTCATG CTGAGCGGTG TCTCCCCGTT CCTGGATGAA 
AGCAAAGAGG AGACGTGTAT CAATGTGTGC AGAGTCGATT TCAGCTTCCC GCCCGAGTAC 
TTCTCTGATG TGAGCCATGC TGCTAGGGAT TTCATCAACG TCATCCTCCA GGAAGACTCC 
AGGAGGAGGC CGACAGCAGC CACTTGTTTA CAGCACCCAT GGCTGCAGCC ACACAATGGC 
AGCTACTCCA AAATCCCGCT GGATACCTCC CGCTTGGCAT CCTTCATTGA GCGCCGCAGA 
CACCAGTACG ACGTGCACCC CGTCCCCGGT GTCAAGAGTT TCCTGATGAG CAGGCTGAAC 
CCCGGGACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KALRN ( XM_015289974.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145460.1 Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X1, mRNA.

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CloneID	OGa38671
Clone ID Related Accession (Same CDS sequence)	XM_015289974.1 , XM_015289974.2
Accession Version	XM_015289974.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	kalirin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGCATT TCGTCTACGG CAGAGACACA 
GGACAGTTGT CTGGAGGGTG TGAACTAACC GTGGTGCTCC AGGACTTCAC GGCGGGCCAC 
AGCAGCGAGC TGAGCATTCA GGTGGGGCAG ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA 
AGGCCAGGCT GGTGCTTGGT GCGGACCACG GAGCGGAGCC CACCTCAGGA AGGCCTGGTC 
CCCAGCAGCG CTCTCTGCAT TTCCCATTCC CGCAGCAGCG TGGAGATGGA TTGCTTCTTC 
CCTTCAGGAA AAGAGGCATA TTCAGTCTCT TCCAATGAAA ACGGGGGCAA GTCCGATTCA 
GTGGCCAACT TACAGCCCCA GCCATCCCTC AACTCCATCC AGAGCTCTCC CGGCCCCAAG 
CGCTCGACCA ACACGCTGAA GAAATGGCTG ACGAGCCCCG TGCGCCGGCT GAACAGCGGG 
AAGGCCGAGA GCAACATCAA GAAGCAAAAG AAGGTGCGAG ATGGCAGGAA GAGCTTTGAC 
CTCGGCTCGC CGAAGACTGG AGATGAAACC ACTCCTCAAG GAGACAGCGC GGATGAGAAG 
AGCAAGAAGG GTTGGGGAGA AGATGAGCCA GATGAAGAGT CTCACACACC TCTTCCTCCT 
CCTATGAAGA TTTTTGACAA TGATCCAACC CAAGATGAGA TGTCCTCTTC TTTGCTAGCT 
GCTCGGCAGA GCTCCTCCGA TGTCCCAACT GCTGCGGATC TTGTCAGTGC AATAGAACAG 
TTGGTCAAAA GTAAGCTGAC CCTTGAAGGA GGATCTTACC GAGGAAGCTT AAAAGATGCC 
ACAGGCTGCT TGAATGAAGG AATGGCGCCT TCAACTCCCC CAAGAAACCT TGAAGAGGAA 
CAAAAAGCCA AAGCAATGAG AGGCAGGATG TTTGTCTTAA ATGAACTGGT CCAGACTGAA 
AAAGACTACG TCAAAGACTT GGGAACCGTT GTGGAGGGCT TCATGAAGAG AATAGAAGAA 
AAGGGAGTTT CCGAAGATAT GAAAGGGAAA GACAAGATAG TGTTTGGCAA TATTCACCAG 
ATCTATGACT GGCACAAAGA CTTTTTCCTG GCTGAACTGG AAAAATGTCT TCAGGAGCCT 
GACCGTTTAG CACAGCTTTT TATTAAACAT GAGCGGCGAT TACACATGTA TGTGGTGTAC 
TGCCAAAACA AGCCACGATC TGAATATATA GTAGCTGAAT ACGAGGCATA TTTTGAGGAG 
GTTCAACAGG AAATAAACCA ACGGCTGACC ATCAGTGACT TTCTGATCAA ACCCATTCAG 
AGAATAACTA AATATCAGCT TCTTCTCAAG GACTTCCTGA GGTACAGTGA AAAAGCTGGT 
CTGGAGTGCT CTGAAATAGA GAAAGCAGTT GAGTTAATGT GCCTTGTACC AAAACGTTGC 
AATGATATGA TGAACCTGGG TCGCCTCCAA GGCTTTGAGG GTAAGCTGAC AGCCCAGGGC 
AAGCTGCTGC AGCAGGACAC CTTCTACGTG ACAGAGCATG ACTCCGGAGT TCAGTCCCGA 
CCAAAGGAAC GTAGGGTGTT TCTCTTTGAG CAAATAGTTA TCTTCAGCGA GCTGCTTAGG 
AAAGGGTCTT TAACACCAGG CTACATGTTC AAGAGAAGCA TAAAGATGAA CTACCTTGTC 
ATTGAAGAAA ATGTGGATGG TGATCCCTGC AAGTTTGCTC TAATGAATCG GGAAACATCT 
GAGAAAGTTG TTTTACAGGC AGCTAATCCA GAAATTCAGC AGGCTTGGGT ACAGGACATC 
AACCAGGTCC TGGACACACA AAGGGATTTC CTAAATGCAC TGCAGTCTCC CATAGAGTAC 
CAACGCAAAG AAAGTAGCAC AGCAGTGCTG AGGCCCCAGG CTGGTAGGGT CGCTCAGCCC 
AACAGCAGAC CCTATTCTTC TGTTCCAGTA GGGTCTGAGA AGCCTTTGAA GGCTACAAGC 
CGTAACCCAT CTCTCCCACC CCTGAAGATA TCTACCTCCA ACGGCAGTAC AGGGTATGAA 
CAGCCCGGAG ACAAGTATGA ACAAAGCAAG ACTGATTTGG GAGGGTGCAA TGGGACATCT 
TCCATGGTGG TGATTAAAGA TTACTATGCA GTGAAGGAGG ACGAGATCTG TGTGAATCAA 
GGAGAGGTGG TTCAGATCCT TGCTATCAAC CAGCAAAACA TGTTCCTGGT GTACCAGCCT 
GCAAATGACC ACTCTCCAGC TGCTGAAGGA TGGATCCCTG GCAATATCTT GGCTCCCCTC 
ACTAAACCCA CTACAGATAA TAGCGACGGA AGCATCAAGA AGTCATGTTC ATGGCATACC 
CTGCGCATGA GAAAGCGGGC GGAAAAGGAG AGCAGTGGCA AAAATGAAGC CAATGGGCCA 
CGTAAATCCA AGGATATTCT GGGGAACAAA GTCTCTGTTA AAGAGACAAA CAGCTCAGAG 
GAATCAGAGT GTGATGACCT TGACCCCAAT ACTAGCATGG AGATAATCAA CCCCAATTTC 
ATTCAGGAAG TGGCTCCAGA GTTCCTTGTG CTGTTGGTGG ATATCACCTG CCTGCTTGGC 
GACACTGTGA TGCTGCAGTG CAAAGTCTGC GGCCGGCCCA AACCAACAAT AACCTGGAAA 
GGACCTGATC AAAACATTCT TGACAATGAC AACAGCACGG CCACATACAC GGTGTCATCC 
TGTGACTCTG GAGAGCTCAT GCTGAAGATC TGCAATGTGA TGCCTCAGGA CAGTGGCATT 
TACACTTGTG TGGCAACTAA CGAGCATGGA ACGGCATCAA CCTCAGCCAC AATCAAAGTG 
CAAGGTGTAC CGGCTGCCCC AAATCGCCCC ATTGCTCAGG AGAGAAGCTG CACCTCTGTG 
ATCCTTCGCT GGCTGCCTCC ATCCAGTACA GGCAATTGCA CCATTTCTGG CTACACAGTG 
GAGTACAGAG AAGAAGGCTC ACAGGTCTGG CAGCAGTCAG TGGCCTCCAC ACTGGACACT 
TACCTTGTCA TTGAAGACCT GTCCCCAGGC TGCCAGTATC AGTTCCGTGT GAGTGCCAGC 
AACCCCTGGG GGATCAGCTT GCCCAGTGAC CCATCTGAAT TCGTGAGGCT TCCAGAGTAT 
GATTCTGCTG CTGATGGTGC CACTATTTCG TGGAAGGAAA ACTTTGATCT CGCTTATGCG 
GAGCTGCATG AGATTGGGAG GGGACGATTC TCCATTGTAA AGAAATGCGT TCACAAAGCT 
ACCCGGAAAG ACGTGGCTGT AAAATTCATC AGTAAAAAAA TGAAGAAGAA GGAGCAAGCA 
GCCCACGAGG CAGCTCTGTT ACAGCACTTA CAGCATCCTC AGTACATCAC TATCCATGAT 
ACCTATGAGT CTCCTACTTC TTACATCCTA GTCCTGGAGC TGATGGATGA TGGTCGTCTC 
CTAGATTATC TAATGAACCA TGATGAACTT ATGGAGGAAA AAGTAGCTTT CTACATAAGA 
GACACAATGG AAGCCTTGCA GTATCTTCAC AATTGCCGAG TGGCTCATTT AGATATAAAG 
CCAGAGAATC TGCTCATCGA TTTAAGGATC CCAGTTCCTC GTGTCAAGAT CATTGATTTG 
GAAGATGCAG TTCAAATCAC AGGCCATTAC CACGTCCACC ATCTCCTTGG AAACCCAGAG 
TTTGCAGCCC CTGAAGTTAT CCAGGGCCTC CCCGTGTCCC TGAGCACAGA CATCTGGAGC 
ATCGGGGTCC TCACCTACGT CATGCTGAGC GGTGTCTCCC CGTTCCTGGA TGAAAGCAAA 
GAGGAGACGT GTATCAATGT GTGCAGAGTC GATTTCAGCT TCCCGCCCGA GTACTTCTCT 
GATGTGAGCC ATGCTGCTAG GGATTTCATC AACGTCATCC TCCAGGAAGA CTCCAGGAGG 
AGGCCGACAG CAGCCACTTG TTTACAGCAC CCATGGCTGC AGCCACACAA TGGCAGCTAC 
TCCAAAATCC CGCTGGATAC CTCCCGCTTG GCATCCTTCA TTGAGCGCCG CAGACACCAG 
TACGACGTGC ACCCCGTCCC CGGTGTCAAG AGTTTCCTGA TGAGCAGGCT GAACCCCGGG 
ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145460.1
CDS	234..9179
Translation	MNPTEGAAEEGAVPDSEVDAFFRTGSFRNDGLKAADVLPILKEKVAFVSGGRDKRGGPVLTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLAKLVDPSQLTDEFEGSLDYNHDEWIELRVSLEEFFNSAIHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVIKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKELLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAVSNNKTPHNSSISHIESVLQQLDEAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDIDLATQVQELLEFLHEKQHELELNADQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEVLLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEIDHVIPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPGVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHNSTGESAEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKRYRDFSLRMGKYRYSLEKALGINTEDNKDLELDIIPASLSDREVKLRDANHEVNEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPAGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSSQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDSFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASSIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKQRNNSRRDGVDDADSQGDGSSQPDTISIASRTSQNTVDSDKHFVYGRDTGQLSGGCELTVVLQDFTAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPSGKEAYSVSSNENGGKSDSVANLQPQPSLNSIQSSPGPKRSTNTLKKWLTSPVRRLNSGKAESNIKKQKKVRDGRKSFDLGSPKTGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSSLLAARQSSSDVPTAADLVSAIEQLVKSKLTLEGGSYRGSLKDATGCLNEGMAPSTPPRNLEEEQKAKAMRGRMFVLNELVQTEKDYVKDLGTVVEGFMKRIEEKGVSEDMKGKDKIVFGNIHQIYDWHKDFFLAELEKCLQEPDRLAQLFIKHERRLHMYVVYCQNKPRSEYIVAEYEAYFEEVQQEINQRLTISDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSEIEKAVELMCLVPKRCNDMMNLGRLQGFEGKLTAQGKLLQQDTFYVTEHDSGVQSRPKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVIEENVDGDPCKFALMNRETSEKVVLQAANPEIQQAWVQDINQVLDTQRDFLNALQSPIEYQRKESSTAVLRPQAGRVAQPNSRPYSSVPVGSEKPLKATSRNPSLPPLKISTSNGSTGYEQPGDKYEQSKTDLGGCNGTSSMVVIKDYYAVKEDEICVNQGEVVQILAINQQNMFLVYQPANDHSPAAEGWIPGNILAPLTKPTTDNSDGSIKKSCSWHTLRMRKRAEKESSGKNEANGPRKSKDILGNKVSVKETNSSEESECDDLDPNTSMEIINPNFIQEVAPEFLVLLVDITCLLGDTVMLQCKVCGRPKPTITWKGPDQNILDNDNSTATYTVSSCDSGELMLKICNVMPQDSGIYTCVATNEHGTASTSATIKVQGVPAAPNRPIAQERSCTSVILRWLPPSSTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCQYQFRVSASNPWGISLPSDPSEFVRLPEYDSAADGATISWKENFDLAYAELHEIGRGRFSIVKKCVHKATRKDVAVKFISKKMKKKEQAAHEAALLQHLQHPQYITIHDTYESPTSYILVLELMDDGRLLDYLMNHDELMEEKVAFYIRDTMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKIIDLEDAVQITGHYHVHHLLGNPEFAAPEVIQGLPVSLSTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPPEYFSDVSHAARDFINVILQEDSRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLASFIERRRHQYDVHPVPGVKSFLMSRLNPGT

Target ORF information:

RefSeq Version	XM_015289974.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289974.1

ORF Insert Sequence:

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGCATT TCGTCTACGG CAGAGACACA 
GGACAGTTGT CTGGAGGGTG TGAACTAACC GTGGTGCTCC AGGACTTCAC GGCGGGCCAC 
AGCAGCGAGC TGAGCATTCA GGTGGGGCAG ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA 
AGGCCAGGCT GGTGCTTGGT GCGGACCACG GAGCGGAGCC CACCTCAGGA AGGCCTGGTC 
CCCAGCAGCG CTCTCTGCAT TTCCCATTCC CGCAGCAGCG TGGAGATGGA TTGCTTCTTC 
CCTTCAGGAA AAGAGGCATA TTCAGTCTCT TCCAATGAAA ACGGGGGCAA GTCCGATTCA 
GTGGCCAACT TACAGCCCCA GCCATCCCTC AACTCCATCC AGAGCTCTCC CGGCCCCAAG 
CGCTCGACCA ACACGCTGAA GAAATGGCTG ACGAGCCCCG TGCGCCGGCT GAACAGCGGG 
AAGGCCGAGA GCAACATCAA GAAGCAAAAG AAGGTGCGAG ATGGCAGGAA GAGCTTTGAC 
CTCGGCTCGC CGAAGACTGG AGATGAAACC ACTCCTCAAG GAGACAGCGC GGATGAGAAG 
AGCAAGAAGG GTTGGGGAGA AGATGAGCCA GATGAAGAGT CTCACACACC TCTTCCTCCT 
CCTATGAAGA TTTTTGACAA TGATCCAACC CAAGATGAGA TGTCCTCTTC TTTGCTAGCT 
GCTCGGCAGA GCTCCTCCGA TGTCCCAACT GCTGCGGATC TTGTCAGTGC AATAGAACAG 
TTGGTCAAAA GTAAGCTGAC CCTTGAAGGA GGATCTTACC GAGGAAGCTT AAAAGATGCC 
ACAGGCTGCT TGAATGAAGG AATGGCGCCT TCAACTCCCC CAAGAAACCT TGAAGAGGAA 
CAAAAAGCCA AAGCAATGAG AGGCAGGATG TTTGTCTTAA ATGAACTGGT CCAGACTGAA 
AAAGACTACG TCAAAGACTT GGGAACCGTT GTGGAGGGCT TCATGAAGAG AATAGAAGAA 
AAGGGAGTTT CCGAAGATAT GAAAGGGAAA GACAAGATAG TGTTTGGCAA TATTCACCAG 
ATCTATGACT GGCACAAAGA CTTTTTCCTG GCTGAACTGG AAAAATGTCT TCAGGAGCCT 
GACCGTTTAG CACAGCTTTT TATTAAACAT GAGCGGCGAT TACACATGTA TGTGGTGTAC 
TGCCAAAACA AGCCACGATC TGAATATATA GTAGCTGAAT ACGAGGCATA TTTTGAGGAG 
GTTCAACAGG AAATAAACCA ACGGCTGACC ATCAGTGACT TTCTGATCAA ACCCATTCAG 
AGAATAACTA AATATCAGCT TCTTCTCAAG GACTTCCTGA GGTACAGTGA AAAAGCTGGT 
CTGGAGTGCT CTGAAATAGA GAAAGCAGTT GAGTTAATGT GCCTTGTACC AAAACGTTGC 
AATGATATGA TGAACCTGGG TCGCCTCCAA GGCTTTGAGG GTAAGCTGAC AGCCCAGGGC 
AAGCTGCTGC AGCAGGACAC CTTCTACGTG ACAGAGCATG ACTCCGGAGT TCAGTCCCGA 
CCAAAGGAAC GTAGGGTGTT TCTCTTTGAG CAAATAGTTA TCTTCAGCGA GCTGCTTAGG 
AAAGGGTCTT TAACACCAGG CTACATGTTC AAGAGAAGCA TAAAGATGAA CTACCTTGTC 
ATTGAAGAAA ATGTGGATGG TGATCCCTGC AAGTTTGCTC TAATGAATCG GGAAACATCT 
GAGAAAGTTG TTTTACAGGC AGCTAATCCA GAAATTCAGC AGGCTTGGGT ACAGGACATC 
AACCAGGTCC TGGACACACA AAGGGATTTC CTAAATGCAC TGCAGTCTCC CATAGAGTAC 
CAACGCAAAG AAAGTAGCAC AGCAGTGCTG AGGCCCCAGG CTGGTAGGGT CGCTCAGCCC 
AACAGCAGAC CCTATTCTTC TGTTCCAGTA GGGTCTGAGA AGCCTTTGAA GGCTACAAGC 
CGTAACCCAT CTCTCCCACC CCTGAAGATA TCTACCTCCA ACGGCAGTAC AGGGTATGAA 
CAGCCCGGAG ACAAGTATGA ACAAAGCAAG ACTGATTTGG GAGGGTGCAA TGGGACATCT 
TCCATGGTGG TGATTAAAGA TTACTATGCA GTGAAGGAGG ACGAGATCTG TGTGAATCAA 
GGAGAGGTGG TTCAGATCCT TGCTATCAAC CAGCAAAACA TGTTCCTGGT GTACCAGCCT 
GCAAATGACC ACTCTCCAGC TGCTGAAGGA TGGATCCCTG GCAATATCTT GGCTCCCCTC 
ACTAAACCCA CTACAGATAA TAGCGACGGA AGCATCAAGA AGTCATGTTC ATGGCATACC 
CTGCGCATGA GAAAGCGGGC GGAAAAGGAG AGCAGTGGCA AAAATGAAGC CAATGGGCCA 
CGTAAATCCA AGGATATTCT GGGGAACAAA GTCTCTGTTA AAGAGACAAA CAGCTCAGAG 
GAATCAGAGT GTGATGACCT TGACCCCAAT ACTAGCATGG AGATAATCAA CCCCAATTTC 
ATTCAGGAAG TGGCTCCAGA GTTCCTTGTG CTGTTGGTGG ATATCACCTG CCTGCTTGGC 
GACACTGTGA TGCTGCAGTG CAAAGTCTGC GGCCGGCCCA AACCAACAAT AACCTGGAAA 
GGACCTGATC AAAACATTCT TGACAATGAC AACAGCACGG CCACATACAC GGTGTCATCC 
TGTGACTCTG GAGAGCTCAT GCTGAAGATC TGCAATGTGA TGCCTCAGGA CAGTGGCATT 
TACACTTGTG TGGCAACTAA CGAGCATGGA ACGGCATCAA CCTCAGCCAC AATCAAAGTG 
CAAGGTGTAC CGGCTGCCCC AAATCGCCCC ATTGCTCAGG AGAGAAGCTG CACCTCTGTG 
ATCCTTCGCT GGCTGCCTCC ATCCAGTACA GGCAATTGCA CCATTTCTGG CTACACAGTG 
GAGTACAGAG AAGAAGGCTC ACAGGTCTGG CAGCAGTCAG TGGCCTCCAC ACTGGACACT 
TACCTTGTCA TTGAAGACCT GTCCCCAGGC TGCCAGTATC AGTTCCGTGT GAGTGCCAGC 
AACCCCTGGG GGATCAGCTT GCCCAGTGAC CCATCTGAAT TCGTGAGGCT TCCAGAGTAT 
GATTCTGCTG CTGATGGTGC CACTATTTCG TGGAAGGAAA ACTTTGATCT CGCTTATGCG 
GAGCTGCATG AGATTGGGAG GGGACGATTC TCCATTGTAA AGAAATGCGT TCACAAAGCT 
ACCCGGAAAG ACGTGGCTGT AAAATTCATC AGTAAAAAAA TGAAGAAGAA GGAGCAAGCA 
GCCCACGAGG CAGCTCTGTT ACAGCACTTA CAGCATCCTC AGTACATCAC TATCCATGAT 
ACCTATGAGT CTCCTACTTC TTACATCCTA GTCCTGGAGC TGATGGATGA TGGTCGTCTC 
CTAGATTATC TAATGAACCA TGATGAACTT ATGGAGGAAA AAGTAGCTTT CTACATAAGA 
GACACAATGG AAGCCTTGCA GTATCTTCAC AATTGCCGAG TGGCTCATTT AGATATAAAG 
CCAGAGAATC TGCTCATCGA TTTAAGGATC CCAGTTCCTC GTGTCAAGAT CATTGATTTG 
GAAGATGCAG TTCAAATCAC AGGCCATTAC CACGTCCACC ATCTCCTTGG AAACCCAGAG 
TTTGCAGCCC CTGAAGTTAT CCAGGGCCTC CCCGTGTCCC TGAGCACAGA CATCTGGAGC 
ATCGGGGTCC TCACCTACGT CATGCTGAGC GGTGTCTCCC CGTTCCTGGA TGAAAGCAAA 
GAGGAGACGT GTATCAATGT GTGCAGAGTC GATTTCAGCT TCCCGCCCGA GTACTTCTCT 
GATGTGAGCC ATGCTGCTAG GGATTTCATC AACGTCATCC TCCAGGAAGA CTCCAGGAGG 
AGGCCGACAG CAGCCACTTG TTTACAGCAC CCATGGCTGC AGCCACACAA TGGCAGCTAC 
TCCAAAATCC CGCTGGATAC CTCCCGCTTG GCATCCTTCA TTGAGCGCCG CAGACACCAG 
TACGACGTGC ACCCCGTCCC CGGTGTCAAG AGTTTCCTGA TGAGCAGGCT GAACCCCGGG 
ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KALRN ( XM_015289974.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145460.1 Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X1, mRNA.

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CloneID	OGa38671
Clone ID Related Accession (Same CDS sequence)	XM_015289974.1 , XM_015289974.2
Accession Version	XM_015289974.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	kalirin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289974.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGCATT TCGTCTACGG CAGAGACACA 
GGACAGTTGT CTGGAGGGTG TGAACTAACC GTGGTGCTCC AGGACTTCAC GGCGGGCCAC 
AGCAGCGAGC TGAGCATTCA GGTGGGGCAG ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA 
AGGCCAGGCT GGTGCTTGGT GCGGACCACG GAGCGGAGCC CACCTCAGGA AGGCCTGGTC 
CCCAGCAGCG CTCTCTGCAT TTCCCATTCC CGCAGCAGCG TGGAGATGGA TTGCTTCTTC 
CCTTCAGGAA AAGAGGCATA TTCAGTCTCT TCCAATGAAA ACGGGGGCAA GTCCGATTCA 
GTGGCCAACT TACAGCCCCA GCCATCCCTC AACTCCATCC AGAGCTCTCC CGGCCCCAAG 
CGCTCGACCA ACACGCTGAA GAAATGGCTG ACGAGCCCCG TGCGCCGGCT GAACAGCGGG 
AAGGCCGAGA GCAACATCAA GAAGCAAAAG AAGGTGCGAG ATGGCAGGAA GAGCTTTGAC 
CTCGGCTCGC CGAAGACTGG AGATGAAACC ACTCCTCAAG GAGACAGCGC GGATGAGAAG 
AGCAAGAAGG GTTGGGGAGA AGATGAGCCA GATGAAGAGT CTCACACACC TCTTCCTCCT 
CCTATGAAGA TTTTTGACAA TGATCCAACC CAAGATGAGA TGTCCTCTTC TTTGCTAGCT 
GCTCGGCAGA GCTCCTCCGA TGTCCCAACT GCTGCGGATC TTGTCAGTGC AATAGAACAG 
TTGGTCAAAA GTAAGCTGAC CCTTGAAGGA GGATCTTACC GAGGAAGCTT AAAAGATGCC 
ACAGGCTGCT TGAATGAAGG AATGGCGCCT TCAACTCCCC CAAGAAACCT TGAAGAGGAA 
CAAAAAGCCA AAGCAATGAG AGGCAGGATG TTTGTCTTAA ATGAACTGGT CCAGACTGAA 
AAAGACTACG TCAAAGACTT GGGAACCGTT GTGGAGGGCT TCATGAAGAG AATAGAAGAA 
AAGGGAGTTT CCGAAGATAT GAAAGGGAAA GACAAGATAG TGTTTGGCAA TATTCACCAG 
ATCTATGACT GGCACAAAGA CTTTTTCCTG GCTGAACTGG AAAAATGTCT TCAGGAGCCT 
GACCGTTTAG CACAGCTTTT TATTAAACAT GAGCGGCGAT TACACATGTA TGTGGTGTAC 
TGCCAAAACA AGCCACGATC TGAATATATA GTAGCTGAAT ACGAGGCATA TTTTGAGGAG 
GTTCAACAGG AAATAAACCA ACGGCTGACC ATCAGTGACT TTCTGATCAA ACCCATTCAG 
AGAATAACTA AATATCAGCT TCTTCTCAAG GACTTCCTGA GGTACAGTGA AAAAGCTGGT 
CTGGAGTGCT CTGAAATAGA GAAAGCAGTT GAGTTAATGT GCCTTGTACC AAAACGTTGC 
AATGATATGA TGAACCTGGG TCGCCTCCAA GGCTTTGAGG GTAAGCTGAC AGCCCAGGGC 
AAGCTGCTGC AGCAGGACAC CTTCTACGTG ACAGAGCATG ACTCCGGAGT TCAGTCCCGA 
CCAAAGGAAC GTAGGGTGTT TCTCTTTGAG CAAATAGTTA TCTTCAGCGA GCTGCTTAGG 
AAAGGGTCTT TAACACCAGG CTACATGTTC AAGAGAAGCA TAAAGATGAA CTACCTTGTC 
ATTGAAGAAA ATGTGGATGG TGATCCCTGC AAGTTTGCTC TAATGAATCG GGAAACATCT 
GAGAAAGTTG TTTTACAGGC AGCTAATCCA GAAATTCAGC AGGCTTGGGT ACAGGACATC 
AACCAGGTCC TGGACACACA AAGGGATTTC CTAAATGCAC TGCAGTCTCC CATAGAGTAC 
CAACGCAAAG AAAGTAGCAC AGCAGTGCTG AGGCCCCAGG CTGGTAGGGT CGCTCAGCCC 
AACAGCAGAC CCTATTCTTC TGTTCCAGTA GGGTCTGAGA AGCCTTTGAA GGCTACAAGC 
CGTAACCCAT CTCTCCCACC CCTGAAGATA TCTACCTCCA ACGGCAGTAC AGGGTATGAA 
CAGCCCGGAG ACAAGTATGA ACAAAGCAAG ACTGATTTGG GAGGGTGCAA TGGGACATCT 
TCCATGGTGG TGATTAAAGA TTACTATGCA GTGAAGGAGG ACGAGATCTG TGTGAATCAA 
GGAGAGGTGG TTCAGATCCT TGCTATCAAC CAGCAAAACA TGTTCCTGGT GTACCAGCCT 
GCAAATGACC ACTCTCCAGC TGCTGAAGGA TGGATCCCTG GCAATATCTT GGCTCCCCTC 
ACTAAACCCA CTACAGATAA TAGCGACGGA AGCATCAAGA AGTCATGTTC ATGGCATACC 
CTGCGCATGA GAAAGCGGGC GGAAAAGGAG AGCAGTGGCA AAAATGAAGC CAATGGGCCA 
CGTAAATCCA AGGATATTCT GGGGAACAAA GTCTCTGTTA AAGAGACAAA CAGCTCAGAG 
GAATCAGAGT GTGATGACCT TGACCCCAAT ACTAGCATGG AGATAATCAA CCCCAATTTC 
ATTCAGGAAG TGGCTCCAGA GTTCCTTGTG CTGTTGGTGG ATATCACCTG CCTGCTTGGC 
GACACTGTGA TGCTGCAGTG CAAAGTCTGC GGCCGGCCCA AACCAACAAT AACCTGGAAA 
GGACCTGATC AAAACATTCT TGACAATGAC AACAGCACGG CCACATACAC GGTGTCATCC 
TGTGACTCTG GAGAGCTCAT GCTGAAGATC TGCAATGTGA TGCCTCAGGA CAGTGGCATT 
TACACTTGTG TGGCAACTAA CGAGCATGGA ACGGCATCAA CCTCAGCCAC AATCAAAGTG 
CAAGGTGTAC CGGCTGCCCC AAATCGCCCC ATTGCTCAGG AGAGAAGCTG CACCTCTGTG 
ATCCTTCGCT GGCTGCCTCC ATCCAGTACA GGCAATTGCA CCATTTCTGG CTACACAGTG 
GAGTACAGAG AAGAAGGCTC ACAGGTCTGG CAGCAGTCAG TGGCCTCCAC ACTGGACACT 
TACCTTGTCA TTGAAGACCT GTCCCCAGGC TGCCAGTATC AGTTCCGTGT GAGTGCCAGC 
AACCCCTGGG GGATCAGCTT GCCCAGTGAC CCATCTGAAT TCGTGAGGCT TCCAGAGTAT 
GATTCTGCTG CTGATGGTGC CACTATTTCG TGGAAGGAAA ACTTTGATCT CGCTTATGCG 
GAGCTGCATG AGATTGGGAG GGGACGATTC TCCATTGTAA AGAAATGCGT TCACAAAGCT 
ACCCGGAAAG ACGTGGCTGT AAAATTCATC AGTAAAAAAA TGAAGAAGAA GGAGCAAGCA 
GCCCACGAGG CAGCTCTGTT ACAGCACTTA CAGCATCCTC AGTACATCAC TATCCATGAT 
ACCTATGAGT CTCCTACTTC TTACATCCTA GTCCTGGAGC TGATGGATGA TGGTCGTCTC 
CTAGATTATC TAATGAACCA TGATGAACTT ATGGAGGAAA AAGTAGCTTT CTACATAAGA 
GACACAATGG AAGCCTTGCA GTATCTTCAC AATTGCCGAG TGGCTCATTT AGATATAAAG 
CCAGAGAATC TGCTCATCGA TTTAAGGATC CCAGTTCCTC GTGTCAAGAT CATTGATTTG 
GAAGATGCAG TTCAAATCAC AGGCCATTAC CACGTCCACC ATCTCCTTGG AAACCCAGAG 
TTTGCAGCCC CTGAAGTTAT CCAGGGCCTC CCCGTGTCCC TGAGCACAGA CATCTGGAGC 
ATCGGGGTCC TCACCTACGT CATGCTGAGC GGTGTCTCCC CGTTCCTGGA TGAAAGCAAA 
GAGGAGACGT GTATCAATGT GTGCAGAGTC GATTTCAGCT TCCCGCCCGA GTACTTCTCT 
GATGTGAGCC ATGCTGCTAG GGATTTCATC AACGTCATCC TCCAGGAAGA CTCCAGGAGG 
AGGCCGACAG CAGCCACTTG TTTACAGCAC CCATGGCTGC AGCCACACAA TGGCAGCTAC 
TCCAAAATCC CGCTGGATAC CTCCCGCTTG GCATCCTTCA TTGAGCGCCG CAGACACCAG 
TACGACGTGC ACCCCGTCCC CGGTGTCAAG AGTTTCCTGA TGAGCAGGCT GAACCCCGGG 
ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145460.1
CDS	244..9189
Translation	MNPTEGAAEEGAVPDSEVDAFFRTGSFRNDGLKAADVLPILKEKVAFVSGGRDKRGGPVLTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLAKLVDPSQLTDEFEGSLDYNHDEWIELRVSLEEFFNSAIHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVIKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKELLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAVSNNKTPHNSSISHIESVLQQLDEAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDIDLATQVQELLEFLHEKQHELELNADQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEVLLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEIDHVIPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPGVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHNSTGESAEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKRYRDFSLRMGKYRYSLEKALGINTEDNKDLELDIIPASLSDREVKLRDANHEVNEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPAGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSSQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDSFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASSIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKQRNNSRRDGVDDADSQGDGSSQPDTISIASRTSQNTVDSDKHFVYGRDTGQLSGGCELTVVLQDFTAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPSGKEAYSVSSNENGGKSDSVANLQPQPSLNSIQSSPGPKRSTNTLKKWLTSPVRRLNSGKAESNIKKQKKVRDGRKSFDLGSPKTGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSSLLAARQSSSDVPTAADLVSAIEQLVKSKLTLEGGSYRGSLKDATGCLNEGMAPSTPPRNLEEEQKAKAMRGRMFVLNELVQTEKDYVKDLGTVVEGFMKRIEEKGVSEDMKGKDKIVFGNIHQIYDWHKDFFLAELEKCLQEPDRLAQLFIKHERRLHMYVVYCQNKPRSEYIVAEYEAYFEEVQQEINQRLTISDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSEIEKAVELMCLVPKRCNDMMNLGRLQGFEGKLTAQGKLLQQDTFYVTEHDSGVQSRPKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVIEENVDGDPCKFALMNRETSEKVVLQAANPEIQQAWVQDINQVLDTQRDFLNALQSPIEYQRKESSTAVLRPQAGRVAQPNSRPYSSVPVGSEKPLKATSRNPSLPPLKISTSNGSTGYEQPGDKYEQSKTDLGGCNGTSSMVVIKDYYAVKEDEICVNQGEVVQILAINQQNMFLVYQPANDHSPAAEGWIPGNILAPLTKPTTDNSDGSIKKSCSWHTLRMRKRAEKESSGKNEANGPRKSKDILGNKVSVKETNSSEESECDDLDPNTSMEIINPNFIQEVAPEFLVLLVDITCLLGDTVMLQCKVCGRPKPTITWKGPDQNILDNDNSTATYTVSSCDSGELMLKICNVMPQDSGIYTCVATNEHGTASTSATIKVQGVPAAPNRPIAQERSCTSVILRWLPPSSTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCQYQFRVSASNPWGISLPSDPSEFVRLPEYDSAADGATISWKENFDLAYAELHEIGRGRFSIVKKCVHKATRKDVAVKFISKKMKKKEQAAHEAALLQHLQHPQYITIHDTYESPTSYILVLELMDDGRLLDYLMNHDELMEEKVAFYIRDTMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKIIDLEDAVQITGHYHVHHLLGNPEFAAPEVIQGLPVSLSTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPPEYFSDVSHAARDFINVILQEDSRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLASFIERRRHQYDVHPVPGVKSFLMSRLNPGT

Target ORF information:

RefSeq Version	XM_015289974.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289974.2

ORF Insert Sequence:

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGCATT TCGTCTACGG CAGAGACACA 
GGACAGTTGT CTGGAGGGTG TGAACTAACC GTGGTGCTCC AGGACTTCAC GGCGGGCCAC 
AGCAGCGAGC TGAGCATTCA GGTGGGGCAG ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA 
AGGCCAGGCT GGTGCTTGGT GCGGACCACG GAGCGGAGCC CACCTCAGGA AGGCCTGGTC 
CCCAGCAGCG CTCTCTGCAT TTCCCATTCC CGCAGCAGCG TGGAGATGGA TTGCTTCTTC 
CCTTCAGGAA AAGAGGCATA TTCAGTCTCT TCCAATGAAA ACGGGGGCAA GTCCGATTCA 
GTGGCCAACT TACAGCCCCA GCCATCCCTC AACTCCATCC AGAGCTCTCC CGGCCCCAAG 
CGCTCGACCA ACACGCTGAA GAAATGGCTG ACGAGCCCCG TGCGCCGGCT GAACAGCGGG 
AAGGCCGAGA GCAACATCAA GAAGCAAAAG AAGGTGCGAG ATGGCAGGAA GAGCTTTGAC 
CTCGGCTCGC CGAAGACTGG AGATGAAACC ACTCCTCAAG GAGACAGCGC GGATGAGAAG 
AGCAAGAAGG GTTGGGGAGA AGATGAGCCA GATGAAGAGT CTCACACACC TCTTCCTCCT 
CCTATGAAGA TTTTTGACAA TGATCCAACC CAAGATGAGA TGTCCTCTTC TTTGCTAGCT 
GCTCGGCAGA GCTCCTCCGA TGTCCCAACT GCTGCGGATC TTGTCAGTGC AATAGAACAG 
TTGGTCAAAA GTAAGCTGAC CCTTGAAGGA GGATCTTACC GAGGAAGCTT AAAAGATGCC 
ACAGGCTGCT TGAATGAAGG AATGGCGCCT TCAACTCCCC CAAGAAACCT TGAAGAGGAA 
CAAAAAGCCA AAGCAATGAG AGGCAGGATG TTTGTCTTAA ATGAACTGGT CCAGACTGAA 
AAAGACTACG TCAAAGACTT GGGAACCGTT GTGGAGGGCT TCATGAAGAG AATAGAAGAA 
AAGGGAGTTT CCGAAGATAT GAAAGGGAAA GACAAGATAG TGTTTGGCAA TATTCACCAG 
ATCTATGACT GGCACAAAGA CTTTTTCCTG GCTGAACTGG AAAAATGTCT TCAGGAGCCT 
GACCGTTTAG CACAGCTTTT TATTAAACAT GAGCGGCGAT TACACATGTA TGTGGTGTAC 
TGCCAAAACA AGCCACGATC TGAATATATA GTAGCTGAAT ACGAGGCATA TTTTGAGGAG 
GTTCAACAGG AAATAAACCA ACGGCTGACC ATCAGTGACT TTCTGATCAA ACCCATTCAG 
AGAATAACTA AATATCAGCT TCTTCTCAAG GACTTCCTGA GGTACAGTGA AAAAGCTGGT 
CTGGAGTGCT CTGAAATAGA GAAAGCAGTT GAGTTAATGT GCCTTGTACC AAAACGTTGC 
AATGATATGA TGAACCTGGG TCGCCTCCAA GGCTTTGAGG GTAAGCTGAC AGCCCAGGGC 
AAGCTGCTGC AGCAGGACAC CTTCTACGTG ACAGAGCATG ACTCCGGAGT TCAGTCCCGA 
CCAAAGGAAC GTAGGGTGTT TCTCTTTGAG CAAATAGTTA TCTTCAGCGA GCTGCTTAGG 
AAAGGGTCTT TAACACCAGG CTACATGTTC AAGAGAAGCA TAAAGATGAA CTACCTTGTC 
ATTGAAGAAA ATGTGGATGG TGATCCCTGC AAGTTTGCTC TAATGAATCG GGAAACATCT 
GAGAAAGTTG TTTTACAGGC AGCTAATCCA GAAATTCAGC AGGCTTGGGT ACAGGACATC 
AACCAGGTCC TGGACACACA AAGGGATTTC CTAAATGCAC TGCAGTCTCC CATAGAGTAC 
CAACGCAAAG AAAGTAGCAC AGCAGTGCTG AGGCCCCAGG CTGGTAGGGT CGCTCAGCCC 
AACAGCAGAC CCTATTCTTC TGTTCCAGTA GGGTCTGAGA AGCCTTTGAA GGCTACAAGC 
CGTAACCCAT CTCTCCCACC CCTGAAGATA TCTACCTCCA ACGGCAGTAC AGGGTATGAA 
CAGCCCGGAG ACAAGTATGA ACAAAGCAAG ACTGATTTGG GAGGGTGCAA TGGGACATCT 
TCCATGGTGG TGATTAAAGA TTACTATGCA GTGAAGGAGG ACGAGATCTG TGTGAATCAA 
GGAGAGGTGG TTCAGATCCT TGCTATCAAC CAGCAAAACA TGTTCCTGGT GTACCAGCCT 
GCAAATGACC ACTCTCCAGC TGCTGAAGGA TGGATCCCTG GCAATATCTT GGCTCCCCTC 
ACTAAACCCA CTACAGATAA TAGCGACGGA AGCATCAAGA AGTCATGTTC ATGGCATACC 
CTGCGCATGA GAAAGCGGGC GGAAAAGGAG AGCAGTGGCA AAAATGAAGC CAATGGGCCA 
CGTAAATCCA AGGATATTCT GGGGAACAAA GTCTCTGTTA AAGAGACAAA CAGCTCAGAG 
GAATCAGAGT GTGATGACCT TGACCCCAAT ACTAGCATGG AGATAATCAA CCCCAATTTC 
ATTCAGGAAG TGGCTCCAGA GTTCCTTGTG CTGTTGGTGG ATATCACCTG CCTGCTTGGC 
GACACTGTGA TGCTGCAGTG CAAAGTCTGC GGCCGGCCCA AACCAACAAT AACCTGGAAA 
GGACCTGATC AAAACATTCT TGACAATGAC AACAGCACGG CCACATACAC GGTGTCATCC 
TGTGACTCTG GAGAGCTCAT GCTGAAGATC TGCAATGTGA TGCCTCAGGA CAGTGGCATT 
TACACTTGTG TGGCAACTAA CGAGCATGGA ACGGCATCAA CCTCAGCCAC AATCAAAGTG 
CAAGGTGTAC CGGCTGCCCC AAATCGCCCC ATTGCTCAGG AGAGAAGCTG CACCTCTGTG 
ATCCTTCGCT GGCTGCCTCC ATCCAGTACA GGCAATTGCA CCATTTCTGG CTACACAGTG 
GAGTACAGAG AAGAAGGCTC ACAGGTCTGG CAGCAGTCAG TGGCCTCCAC ACTGGACACT 
TACCTTGTCA TTGAAGACCT GTCCCCAGGC TGCCAGTATC AGTTCCGTGT GAGTGCCAGC 
AACCCCTGGG GGATCAGCTT GCCCAGTGAC CCATCTGAAT TCGTGAGGCT TCCAGAGTAT 
GATTCTGCTG CTGATGGTGC CACTATTTCG TGGAAGGAAA ACTTTGATCT CGCTTATGCG 
GAGCTGCATG AGATTGGGAG GGGACGATTC TCCATTGTAA AGAAATGCGT TCACAAAGCT 
ACCCGGAAAG ACGTGGCTGT AAAATTCATC AGTAAAAAAA TGAAGAAGAA GGAGCAAGCA 
GCCCACGAGG CAGCTCTGTT ACAGCACTTA CAGCATCCTC AGTACATCAC TATCCATGAT 
ACCTATGAGT CTCCTACTTC TTACATCCTA GTCCTGGAGC TGATGGATGA TGGTCGTCTC 
CTAGATTATC TAATGAACCA TGATGAACTT ATGGAGGAAA AAGTAGCTTT CTACATAAGA 
GACACAATGG AAGCCTTGCA GTATCTTCAC AATTGCCGAG TGGCTCATTT AGATATAAAG 
CCAGAGAATC TGCTCATCGA TTTAAGGATC CCAGTTCCTC GTGTCAAGAT CATTGATTTG 
GAAGATGCAG TTCAAATCAC AGGCCATTAC CACGTCCACC ATCTCCTTGG AAACCCAGAG 
TTTGCAGCCC CTGAAGTTAT CCAGGGCCTC CCCGTGTCCC TGAGCACAGA CATCTGGAGC 
ATCGGGGTCC TCACCTACGT CATGCTGAGC GGTGTCTCCC CGTTCCTGGA TGAAAGCAAA 
GAGGAGACGT GTATCAATGT GTGCAGAGTC GATTTCAGCT TCCCGCCCGA GTACTTCTCT 
GATGTGAGCC ATGCTGCTAG GGATTTCATC AACGTCATCC TCCAGGAAGA CTCCAGGAGG 
AGGCCGACAG CAGCCACTTG TTTACAGCAC CCATGGCTGC AGCCACACAA TGGCAGCTAC 
TCCAAAATCC CGCTGGATAC CTCCCGCTTG GCATCCTTCA TTGAGCGCCG CAGACACCAG 
TACGACGTGC ACCCCGTCCC CGGTGTCAAG AGTTTCCTGA TGAGCAGGCT GAACCCCGGG 
ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KALRN ( XM_015289976.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145462.1 Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X2, mRNA.

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CloneID	OGa38672
Clone ID Related Accession (Same CDS sequence)	XM_015289976.1 , XM_015289976.2
Accession Version	XM_015289976.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	kalirin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGCATT TCGTCTACGG CAGAGACACA 
GGACAGTTGT CTGGAGGGTG TGAACTAACC GTGGTGCTCC AGGACTTCAC GGCGGGCCAC 
AGCAGCGAGC TGAGCATTCA GGTGGGGCAG ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA 
AGGCCAGGCT GGTGCTTGGT GCGGACCACG GAGCGGAGCC CACCTCAGGA AGGCCTGGTC 
CCCAGCAGCG CTCTCTGCAT TTCCCATTCC CGCAGCAGCG TGGAGATGGA TTGCTTCTTC 
CCTTCAGGAA AAGAGGCATA TTCAGTCTCT TCCAATGAAA ACGGGGGCAA GTCCGATTCA 
GTGGCCAACT TACAGCCCCA GCCATCCCTC AACTCCATCC AGAGCTCTCC CGGCCCCAAG 
CGCTCGACCA ACACGCTGAA GAAATGGCTG ACGAGCCCCG TGCGCCGGCT GAACAGCGGG 
AAGGCCGAGA GCAACATCAA GAAGCAAAAG AAGGTGCGAG ATGGCAGGAA GAGCTTTGAC 
CTCGGCTCGC CGAAGACTGG AGATGAAACC ACTCCTCAAG GAGACAGCGC GGATGAGAGC 
AAGAAGGGTT GGGGAGAAGA TGAGCCAGAT GAAGAGTCTC ACACACCTCT TCCTCCTCCT 
ATGAAGATTT TTGACAATGA TCCAACCCAA GATGAGATGT CCTCTTCTTT GCTAGCTGCT 
CGGCAGAGCT CCTCCGATGT CCCAACTGCT GCGGATCTTG TCAGTGCAAT AGAACAGTTG 
GTCAAAAGTA AGCTGACCCT TGAAGGAGGA TCTTACCGAG GAAGCTTAAA AGATGCCACA 
GGCTGCTTGA ATGAAGGAAT GGCGCCTTCA ACTCCCCCAA GAAACCTTGA AGAGGAACAA 
AAAGCCAAAG CAATGAGAGG CAGGATGTTT GTCTTAAATG AACTGGTCCA GACTGAAAAA 
GACTACGTCA AAGACTTGGG AACCGTTGTG GAGGGCTTCA TGAAGAGAAT AGAAGAAAAG 
GGAGTTTCCG AAGATATGAA AGGGAAAGAC AAGATAGTGT TTGGCAATAT TCACCAGATC 
TATGACTGGC ACAAAGACTT TTTCCTGGCT GAACTGGAAA AATGTCTTCA GGAGCCTGAC 
CGTTTAGCAC AGCTTTTTAT TAAACATGAG CGGCGATTAC ACATGTATGT GGTGTACTGC 
CAAAACAAGC CACGATCTGA ATATATAGTA GCTGAATACG AGGCATATTT TGAGGAGGTT 
CAACAGGAAA TAAACCAACG GCTGACCATC AGTGACTTTC TGATCAAACC CATTCAGAGA 
ATAACTAAAT ATCAGCTTCT TCTCAAGGAC TTCCTGAGGT ACAGTGAAAA AGCTGGTCTG 
GAGTGCTCTG AAATAGAGAA AGCAGTTGAG TTAATGTGCC TTGTACCAAA ACGTTGCAAT 
GATATGATGA ACCTGGGTCG CCTCCAAGGC TTTGAGGGTA AGCTGACAGC CCAGGGCAAG 
CTGCTGCAGC AGGACACCTT CTACGTGACA GAGCATGACT CCGGAGTTCA GTCCCGACCA 
AAGGAACGTA GGGTGTTTCT CTTTGAGCAA ATAGTTATCT TCAGCGAGCT GCTTAGGAAA 
GGGTCTTTAA CACCAGGCTA CATGTTCAAG AGAAGCATAA AGATGAACTA CCTTGTCATT 
GAAGAAAATG TGGATGGTGA TCCCTGCAAG TTTGCTCTAA TGAATCGGGA AACATCTGAG 
AAAGTTGTTT TACAGGCAGC TAATCCAGAA ATTCAGCAGG CTTGGGTACA GGACATCAAC 
CAGGTCCTGG ACACACAAAG GGATTTCCTA AATGCACTGC AGTCTCCCAT AGAGTACCAA 
CGCAAAGAAA GTAGCACAGC AGTGCTGAGG CCCCAGGCTG GTAGGGTCGC TCAGCCCAAC 
AGCAGACCCT ATTCTTCTGT TCCAGTAGGG TCTGAGAAGC CTTTGAAGGC TACAAGCCGT 
AACCCATCTC TCCCACCCCT GAAGATATCT ACCTCCAACG GCAGTACAGG GTATGAACAG 
CCCGGAGACA AGTATGAACA AAGCAAGACT GATTTGGGAG GGTGCAATGG GACATCTTCC 
ATGGTGGTGA TTAAAGATTA CTATGCAGTG AAGGAGGACG AGATCTGTGT GAATCAAGGA 
GAGGTGGTTC AGATCCTTGC TATCAACCAG CAAAACATGT TCCTGGTGTA CCAGCCTGCA 
AATGACCACT CTCCAGCTGC TGAAGGATGG ATCCCTGGCA ATATCTTGGC TCCCCTCACT 
AAACCCACTA CAGATAATAG CGACGGAAGC ATCAAGAAGT CATGTTCATG GCATACCCTG 
CGCATGAGAA AGCGGGCGGA AAAGGAGAGC AGTGGCAAAA ATGAAGCCAA TGGGCCACGT 
AAATCCAAGG ATATTCTGGG GAACAAAGTC TCTGTTAAAG AGACAAACAG CTCAGAGGAA 
TCAGAGTGTG ATGACCTTGA CCCCAATACT AGCATGGAGA TAATCAACCC CAATTTCATT 
CAGGAAGTGG CTCCAGAGTT CCTTGTGCTG TTGGTGGATA TCACCTGCCT GCTTGGCGAC 
ACTGTGATGC TGCAGTGCAA AGTCTGCGGC CGGCCCAAAC CAACAATAAC CTGGAAAGGA 
CCTGATCAAA ACATTCTTGA CAATGACAAC AGCACGGCCA CATACACGGT GTCATCCTGT 
GACTCTGGAG AGCTCATGCT GAAGATCTGC AATGTGATGC CTCAGGACAG TGGCATTTAC 
ACTTGTGTGG CAACTAACGA GCATGGAACG GCATCAACCT CAGCCACAAT CAAAGTGCAA 
GGTGTACCGG CTGCCCCAAA TCGCCCCATT GCTCAGGAGA GAAGCTGCAC CTCTGTGATC 
CTTCGCTGGC TGCCTCCATC CAGTACAGGC AATTGCACCA TTTCTGGCTA CACAGTGGAG 
TACAGAGAAG AAGGCTCACA GGTCTGGCAG CAGTCAGTGG CCTCCACACT GGACACTTAC 
CTTGTCATTG AAGACCTGTC CCCAGGCTGC CAGTATCAGT TCCGTGTGAG TGCCAGCAAC 
CCCTGGGGGA TCAGCTTGCC CAGTGACCCA TCTGAATTCG TGAGGCTTCC AGAGTATGAT 
TCTGCTGCTG ATGGTGCCAC TATTTCGTGG AAGGAAAACT TTGATCTCGC TTATGCGGAG 
CTGCATGAGA TTGGGAGGGG ACGATTCTCC ATTGTAAAGA AATGCGTTCA CAAAGCTACC 
CGGAAAGACG TGGCTGTAAA ATTCATCAGT AAAAAAATGA AGAAGAAGGA GCAAGCAGCC 
CACGAGGCAG CTCTGTTACA GCACTTACAG CATCCTCAGT ACATCACTAT CCATGATACC 
TATGAGTCTC CTACTTCTTA CATCCTAGTC CTGGAGCTGA TGGATGATGG TCGTCTCCTA 
GATTATCTAA TGAACCATGA TGAACTTATG GAGGAAAAAG TAGCTTTCTA CATAAGAGAC 
ACAATGGAAG CCTTGCAGTA TCTTCACAAT TGCCGAGTGG CTCATTTAGA TATAAAGCCA 
GAGAATCTGC TCATCGATTT AAGGATCCCA GTTCCTCGTG TCAAGATCAT TGATTTGGAA 
GATGCAGTTC AAATCACAGG CCATTACCAC GTCCACCATC TCCTTGGAAA CCCAGAGTTT 
GCAGCCCCTG AAGTTATCCA GGGCCTCCCC GTGTCCCTGA GCACAGACAT CTGGAGCATC 
GGGGTCCTCA CCTACGTCAT GCTGAGCGGT GTCTCCCCGT TCCTGGATGA AAGCAAAGAG 
GAGACGTGTA TCAATGTGTG CAGAGTCGAT TTCAGCTTCC CGCCCGAGTA CTTCTCTGAT 
GTGAGCCATG CTGCTAGGGA TTTCATCAAC GTCATCCTCC AGGAAGACTC CAGGAGGAGG 
CCGACAGCAG CCACTTGTTT ACAGCACCCA TGGCTGCAGC CACACAATGG CAGCTACTCC 
AAAATCCCGC TGGATACCTC CCGCTTGGCA TCCTTCATTG AGCGCCGCAG ACACCAGTAC 
GACGTGCACC CCGTCCCCGG TGTCAAGAGT TTCCTGATGA GCAGGCTGAA CCCCGGGACG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145462.1
CDS	234..9176
Translation	MNPTEGAAEEGAVPDSEVDAFFRTGSFRNDGLKAADVLPILKEKVAFVSGGRDKRGGPVLTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLAKLVDPSQLTDEFEGSLDYNHDEWIELRVSLEEFFNSAIHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVIKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKELLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAVSNNKTPHNSSISHIESVLQQLDEAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDIDLATQVQELLEFLHEKQHELELNADQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEVLLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEIDHVIPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPGVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHNSTGESAEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKRYRDFSLRMGKYRYSLEKALGINTEDNKDLELDIIPASLSDREVKLRDANHEVNEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPAGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSSQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDSFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASSIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKQRNNSRRDGVDDADSQGDGSSQPDTISIASRTSQNTVDSDKHFVYGRDTGQLSGGCELTVVLQDFTAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPSGKEAYSVSSNENGGKSDSVANLQPQPSLNSIQSSPGPKRSTNTLKKWLTSPVRRLNSGKAESNIKKQKKVRDGRKSFDLGSPKTGDETTPQGDSADESKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSSLLAARQSSSDVPTAADLVSAIEQLVKSKLTLEGGSYRGSLKDATGCLNEGMAPSTPPRNLEEEQKAKAMRGRMFVLNELVQTEKDYVKDLGTVVEGFMKRIEEKGVSEDMKGKDKIVFGNIHQIYDWHKDFFLAELEKCLQEPDRLAQLFIKHERRLHMYVVYCQNKPRSEYIVAEYEAYFEEVQQEINQRLTISDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSEIEKAVELMCLVPKRCNDMMNLGRLQGFEGKLTAQGKLLQQDTFYVTEHDSGVQSRPKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVIEENVDGDPCKFALMNRETSEKVVLQAANPEIQQAWVQDINQVLDTQRDFLNALQSPIEYQRKESSTAVLRPQAGRVAQPNSRPYSSVPVGSEKPLKATSRNPSLPPLKISTSNGSTGYEQPGDKYEQSKTDLGGCNGTSSMVVIKDYYAVKEDEICVNQGEVVQILAINQQNMFLVYQPANDHSPAAEGWIPGNILAPLTKPTTDNSDGSIKKSCSWHTLRMRKRAEKESSGKNEANGPRKSKDILGNKVSVKETNSSEESECDDLDPNTSMEIINPNFIQEVAPEFLVLLVDITCLLGDTVMLQCKVCGRPKPTITWKGPDQNILDNDNSTATYTVSSCDSGELMLKICNVMPQDSGIYTCVATNEHGTASTSATIKVQGVPAAPNRPIAQERSCTSVILRWLPPSSTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCQYQFRVSASNPWGISLPSDPSEFVRLPEYDSAADGATISWKENFDLAYAELHEIGRGRFSIVKKCVHKATRKDVAVKFISKKMKKKEQAAHEAALLQHLQHPQYITIHDTYESPTSYILVLELMDDGRLLDYLMNHDELMEEKVAFYIRDTMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKIIDLEDAVQITGHYHVHHLLGNPEFAAPEVIQGLPVSLSTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPPEYFSDVSHAARDFINVILQEDSRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLASFIERRRHQYDVHPVPGVKSFLMSRLNPGT

Target ORF information:

RefSeq Version	XM_015289976.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289976.1

ORF Insert Sequence:

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGCATT TCGTCTACGG CAGAGACACA 
GGACAGTTGT CTGGAGGGTG TGAACTAACC GTGGTGCTCC AGGACTTCAC GGCGGGCCAC 
AGCAGCGAGC TGAGCATTCA GGTGGGGCAG ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA 
AGGCCAGGCT GGTGCTTGGT GCGGACCACG GAGCGGAGCC CACCTCAGGA AGGCCTGGTC 
CCCAGCAGCG CTCTCTGCAT TTCCCATTCC CGCAGCAGCG TGGAGATGGA TTGCTTCTTC 
CCTTCAGGAA AAGAGGCATA TTCAGTCTCT TCCAATGAAA ACGGGGGCAA GTCCGATTCA 
GTGGCCAACT TACAGCCCCA GCCATCCCTC AACTCCATCC AGAGCTCTCC CGGCCCCAAG 
CGCTCGACCA ACACGCTGAA GAAATGGCTG ACGAGCCCCG TGCGCCGGCT GAACAGCGGG 
AAGGCCGAGA GCAACATCAA GAAGCAAAAG AAGGTGCGAG ATGGCAGGAA GAGCTTTGAC 
CTCGGCTCGC CGAAGACTGG AGATGAAACC ACTCCTCAAG GAGACAGCGC GGATGAGAGC 
AAGAAGGGTT GGGGAGAAGA TGAGCCAGAT GAAGAGTCTC ACACACCTCT TCCTCCTCCT 
ATGAAGATTT TTGACAATGA TCCAACCCAA GATGAGATGT CCTCTTCTTT GCTAGCTGCT 
CGGCAGAGCT CCTCCGATGT CCCAACTGCT GCGGATCTTG TCAGTGCAAT AGAACAGTTG 
GTCAAAAGTA AGCTGACCCT TGAAGGAGGA TCTTACCGAG GAAGCTTAAA AGATGCCACA 
GGCTGCTTGA ATGAAGGAAT GGCGCCTTCA ACTCCCCCAA GAAACCTTGA AGAGGAACAA 
AAAGCCAAAG CAATGAGAGG CAGGATGTTT GTCTTAAATG AACTGGTCCA GACTGAAAAA 
GACTACGTCA AAGACTTGGG AACCGTTGTG GAGGGCTTCA TGAAGAGAAT AGAAGAAAAG 
GGAGTTTCCG AAGATATGAA AGGGAAAGAC AAGATAGTGT TTGGCAATAT TCACCAGATC 
TATGACTGGC ACAAAGACTT TTTCCTGGCT GAACTGGAAA AATGTCTTCA GGAGCCTGAC 
CGTTTAGCAC AGCTTTTTAT TAAACATGAG CGGCGATTAC ACATGTATGT GGTGTACTGC 
CAAAACAAGC CACGATCTGA ATATATAGTA GCTGAATACG AGGCATATTT TGAGGAGGTT 
CAACAGGAAA TAAACCAACG GCTGACCATC AGTGACTTTC TGATCAAACC CATTCAGAGA 
ATAACTAAAT ATCAGCTTCT TCTCAAGGAC TTCCTGAGGT ACAGTGAAAA AGCTGGTCTG 
GAGTGCTCTG AAATAGAGAA AGCAGTTGAG TTAATGTGCC TTGTACCAAA ACGTTGCAAT 
GATATGATGA ACCTGGGTCG CCTCCAAGGC TTTGAGGGTA AGCTGACAGC CCAGGGCAAG 
CTGCTGCAGC AGGACACCTT CTACGTGACA GAGCATGACT CCGGAGTTCA GTCCCGACCA 
AAGGAACGTA GGGTGTTTCT CTTTGAGCAA ATAGTTATCT TCAGCGAGCT GCTTAGGAAA 
GGGTCTTTAA CACCAGGCTA CATGTTCAAG AGAAGCATAA AGATGAACTA CCTTGTCATT 
GAAGAAAATG TGGATGGTGA TCCCTGCAAG TTTGCTCTAA TGAATCGGGA AACATCTGAG 
AAAGTTGTTT TACAGGCAGC TAATCCAGAA ATTCAGCAGG CTTGGGTACA GGACATCAAC 
CAGGTCCTGG ACACACAAAG GGATTTCCTA AATGCACTGC AGTCTCCCAT AGAGTACCAA 
CGCAAAGAAA GTAGCACAGC AGTGCTGAGG CCCCAGGCTG GTAGGGTCGC TCAGCCCAAC 
AGCAGACCCT ATTCTTCTGT TCCAGTAGGG TCTGAGAAGC CTTTGAAGGC TACAAGCCGT 
AACCCATCTC TCCCACCCCT GAAGATATCT ACCTCCAACG GCAGTACAGG GTATGAACAG 
CCCGGAGACA AGTATGAACA AAGCAAGACT GATTTGGGAG GGTGCAATGG GACATCTTCC 
ATGGTGGTGA TTAAAGATTA CTATGCAGTG AAGGAGGACG AGATCTGTGT GAATCAAGGA 
GAGGTGGTTC AGATCCTTGC TATCAACCAG CAAAACATGT TCCTGGTGTA CCAGCCTGCA 
AATGACCACT CTCCAGCTGC TGAAGGATGG ATCCCTGGCA ATATCTTGGC TCCCCTCACT 
AAACCCACTA CAGATAATAG CGACGGAAGC ATCAAGAAGT CATGTTCATG GCATACCCTG 
CGCATGAGAA AGCGGGCGGA AAAGGAGAGC AGTGGCAAAA ATGAAGCCAA TGGGCCACGT 
AAATCCAAGG ATATTCTGGG GAACAAAGTC TCTGTTAAAG AGACAAACAG CTCAGAGGAA 
TCAGAGTGTG ATGACCTTGA CCCCAATACT AGCATGGAGA TAATCAACCC CAATTTCATT 
CAGGAAGTGG CTCCAGAGTT CCTTGTGCTG TTGGTGGATA TCACCTGCCT GCTTGGCGAC 
ACTGTGATGC TGCAGTGCAA AGTCTGCGGC CGGCCCAAAC CAACAATAAC CTGGAAAGGA 
CCTGATCAAA ACATTCTTGA CAATGACAAC AGCACGGCCA CATACACGGT GTCATCCTGT 
GACTCTGGAG AGCTCATGCT GAAGATCTGC AATGTGATGC CTCAGGACAG TGGCATTTAC 
ACTTGTGTGG CAACTAACGA GCATGGAACG GCATCAACCT CAGCCACAAT CAAAGTGCAA 
GGTGTACCGG CTGCCCCAAA TCGCCCCATT GCTCAGGAGA GAAGCTGCAC CTCTGTGATC 
CTTCGCTGGC TGCCTCCATC CAGTACAGGC AATTGCACCA TTTCTGGCTA CACAGTGGAG 
TACAGAGAAG AAGGCTCACA GGTCTGGCAG CAGTCAGTGG CCTCCACACT GGACACTTAC 
CTTGTCATTG AAGACCTGTC CCCAGGCTGC CAGTATCAGT TCCGTGTGAG TGCCAGCAAC 
CCCTGGGGGA TCAGCTTGCC CAGTGACCCA TCTGAATTCG TGAGGCTTCC AGAGTATGAT 
TCTGCTGCTG ATGGTGCCAC TATTTCGTGG AAGGAAAACT TTGATCTCGC TTATGCGGAG 
CTGCATGAGA TTGGGAGGGG ACGATTCTCC ATTGTAAAGA AATGCGTTCA CAAAGCTACC 
CGGAAAGACG TGGCTGTAAA ATTCATCAGT AAAAAAATGA AGAAGAAGGA GCAAGCAGCC 
CACGAGGCAG CTCTGTTACA GCACTTACAG CATCCTCAGT ACATCACTAT CCATGATACC 
TATGAGTCTC CTACTTCTTA CATCCTAGTC CTGGAGCTGA TGGATGATGG TCGTCTCCTA 
GATTATCTAA TGAACCATGA TGAACTTATG GAGGAAAAAG TAGCTTTCTA CATAAGAGAC 
ACAATGGAAG CCTTGCAGTA TCTTCACAAT TGCCGAGTGG CTCATTTAGA TATAAAGCCA 
GAGAATCTGC TCATCGATTT AAGGATCCCA GTTCCTCGTG TCAAGATCAT TGATTTGGAA 
GATGCAGTTC AAATCACAGG CCATTACCAC GTCCACCATC TCCTTGGAAA CCCAGAGTTT 
GCAGCCCCTG AAGTTATCCA GGGCCTCCCC GTGTCCCTGA GCACAGACAT CTGGAGCATC 
GGGGTCCTCA CCTACGTCAT GCTGAGCGGT GTCTCCCCGT TCCTGGATGA AAGCAAAGAG 
GAGACGTGTA TCAATGTGTG CAGAGTCGAT TTCAGCTTCC CGCCCGAGTA CTTCTCTGAT 
GTGAGCCATG CTGCTAGGGA TTTCATCAAC GTCATCCTCC AGGAAGACTC CAGGAGGAGG 
CCGACAGCAG CCACTTGTTT ACAGCACCCA TGGCTGCAGC CACACAATGG CAGCTACTCC 
AAAATCCCGC TGGATACCTC CCGCTTGGCA TCCTTCATTG AGCGCCGCAG ACACCAGTAC 
GACGTGCACC CCGTCCCCGG TGTCAAGAGT TTCCTGATGA GCAGGCTGAA CCCCGGGACG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KALRN ( XM_015289976.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145462.1 Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X2, mRNA.

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CloneID	OGa38672
Clone ID Related Accession (Same CDS sequence)	XM_015289976.1 , XM_015289976.2
Accession Version	XM_015289976.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	kalirin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289976.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGCATT TCGTCTACGG CAGAGACACA 
GGACAGTTGT CTGGAGGGTG TGAACTAACC GTGGTGCTCC AGGACTTCAC GGCGGGCCAC 
AGCAGCGAGC TGAGCATTCA GGTGGGGCAG ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA 
AGGCCAGGCT GGTGCTTGGT GCGGACCACG GAGCGGAGCC CACCTCAGGA AGGCCTGGTC 
CCCAGCAGCG CTCTCTGCAT TTCCCATTCC CGCAGCAGCG TGGAGATGGA TTGCTTCTTC 
CCTTCAGGAA AAGAGGCATA TTCAGTCTCT TCCAATGAAA ACGGGGGCAA GTCCGATTCA 
GTGGCCAACT TACAGCCCCA GCCATCCCTC AACTCCATCC AGAGCTCTCC CGGCCCCAAG 
CGCTCGACCA ACACGCTGAA GAAATGGCTG ACGAGCCCCG TGCGCCGGCT GAACAGCGGG 
AAGGCCGAGA GCAACATCAA GAAGCAAAAG AAGGTGCGAG ATGGCAGGAA GAGCTTTGAC 
CTCGGCTCGC CGAAGACTGG AGATGAAACC ACTCCTCAAG GAGACAGCGC GGATGAGAGC 
AAGAAGGGTT GGGGAGAAGA TGAGCCAGAT GAAGAGTCTC ACACACCTCT TCCTCCTCCT 
ATGAAGATTT TTGACAATGA TCCAACCCAA GATGAGATGT CCTCTTCTTT GCTAGCTGCT 
CGGCAGAGCT CCTCCGATGT CCCAACTGCT GCGGATCTTG TCAGTGCAAT AGAACAGTTG 
GTCAAAAGTA AGCTGACCCT TGAAGGAGGA TCTTACCGAG GAAGCTTAAA AGATGCCACA 
GGCTGCTTGA ATGAAGGAAT GGCGCCTTCA ACTCCCCCAA GAAACCTTGA AGAGGAACAA 
AAAGCCAAAG CAATGAGAGG CAGGATGTTT GTCTTAAATG AACTGGTCCA GACTGAAAAA 
GACTACGTCA AAGACTTGGG AACCGTTGTG GAGGGCTTCA TGAAGAGAAT AGAAGAAAAG 
GGAGTTTCCG AAGATATGAA AGGGAAAGAC AAGATAGTGT TTGGCAATAT TCACCAGATC 
TATGACTGGC ACAAAGACTT TTTCCTGGCT GAACTGGAAA AATGTCTTCA GGAGCCTGAC 
CGTTTAGCAC AGCTTTTTAT TAAACATGAG CGGCGATTAC ACATGTATGT GGTGTACTGC 
CAAAACAAGC CACGATCTGA ATATATAGTA GCTGAATACG AGGCATATTT TGAGGAGGTT 
CAACAGGAAA TAAACCAACG GCTGACCATC AGTGACTTTC TGATCAAACC CATTCAGAGA 
ATAACTAAAT ATCAGCTTCT TCTCAAGGAC TTCCTGAGGT ACAGTGAAAA AGCTGGTCTG 
GAGTGCTCTG AAATAGAGAA AGCAGTTGAG TTAATGTGCC TTGTACCAAA ACGTTGCAAT 
GATATGATGA ACCTGGGTCG CCTCCAAGGC TTTGAGGGTA AGCTGACAGC CCAGGGCAAG 
CTGCTGCAGC AGGACACCTT CTACGTGACA GAGCATGACT CCGGAGTTCA GTCCCGACCA 
AAGGAACGTA GGGTGTTTCT CTTTGAGCAA ATAGTTATCT TCAGCGAGCT GCTTAGGAAA 
GGGTCTTTAA CACCAGGCTA CATGTTCAAG AGAAGCATAA AGATGAACTA CCTTGTCATT 
GAAGAAAATG TGGATGGTGA TCCCTGCAAG TTTGCTCTAA TGAATCGGGA AACATCTGAG 
AAAGTTGTTT TACAGGCAGC TAATCCAGAA ATTCAGCAGG CTTGGGTACA GGACATCAAC 
CAGGTCCTGG ACACACAAAG GGATTTCCTA AATGCACTGC AGTCTCCCAT AGAGTACCAA 
CGCAAAGAAA GTAGCACAGC AGTGCTGAGG CCCCAGGCTG GTAGGGTCGC TCAGCCCAAC 
AGCAGACCCT ATTCTTCTGT TCCAGTAGGG TCTGAGAAGC CTTTGAAGGC TACAAGCCGT 
AACCCATCTC TCCCACCCCT GAAGATATCT ACCTCCAACG GCAGTACAGG GTATGAACAG 
CCCGGAGACA AGTATGAACA AAGCAAGACT GATTTGGGAG GGTGCAATGG GACATCTTCC 
ATGGTGGTGA TTAAAGATTA CTATGCAGTG AAGGAGGACG AGATCTGTGT GAATCAAGGA 
GAGGTGGTTC AGATCCTTGC TATCAACCAG CAAAACATGT TCCTGGTGTA CCAGCCTGCA 
AATGACCACT CTCCAGCTGC TGAAGGATGG ATCCCTGGCA ATATCTTGGC TCCCCTCACT 
AAACCCACTA CAGATAATAG CGACGGAAGC ATCAAGAAGT CATGTTCATG GCATACCCTG 
CGCATGAGAA AGCGGGCGGA AAAGGAGAGC AGTGGCAAAA ATGAAGCCAA TGGGCCACGT 
AAATCCAAGG ATATTCTGGG GAACAAAGTC TCTGTTAAAG AGACAAACAG CTCAGAGGAA 
TCAGAGTGTG ATGACCTTGA CCCCAATACT AGCATGGAGA TAATCAACCC CAATTTCATT 
CAGGAAGTGG CTCCAGAGTT CCTTGTGCTG TTGGTGGATA TCACCTGCCT GCTTGGCGAC 
ACTGTGATGC TGCAGTGCAA AGTCTGCGGC CGGCCCAAAC CAACAATAAC CTGGAAAGGA 
CCTGATCAAA ACATTCTTGA CAATGACAAC AGCACGGCCA CATACACGGT GTCATCCTGT 
GACTCTGGAG AGCTCATGCT GAAGATCTGC AATGTGATGC CTCAGGACAG TGGCATTTAC 
ACTTGTGTGG CAACTAACGA GCATGGAACG GCATCAACCT CAGCCACAAT CAAAGTGCAA 
GGTGTACCGG CTGCCCCAAA TCGCCCCATT GCTCAGGAGA GAAGCTGCAC CTCTGTGATC 
CTTCGCTGGC TGCCTCCATC CAGTACAGGC AATTGCACCA TTTCTGGCTA CACAGTGGAG 
TACAGAGAAG AAGGCTCACA GGTCTGGCAG CAGTCAGTGG CCTCCACACT GGACACTTAC 
CTTGTCATTG AAGACCTGTC CCCAGGCTGC CAGTATCAGT TCCGTGTGAG TGCCAGCAAC 
CCCTGGGGGA TCAGCTTGCC CAGTGACCCA TCTGAATTCG TGAGGCTTCC AGAGTATGAT 
TCTGCTGCTG ATGGTGCCAC TATTTCGTGG AAGGAAAACT TTGATCTCGC TTATGCGGAG 
CTGCATGAGA TTGGGAGGGG ACGATTCTCC ATTGTAAAGA AATGCGTTCA CAAAGCTACC 
CGGAAAGACG TGGCTGTAAA ATTCATCAGT AAAAAAATGA AGAAGAAGGA GCAAGCAGCC 
CACGAGGCAG CTCTGTTACA GCACTTACAG CATCCTCAGT ACATCACTAT CCATGATACC 
TATGAGTCTC CTACTTCTTA CATCCTAGTC CTGGAGCTGA TGGATGATGG TCGTCTCCTA 
GATTATCTAA TGAACCATGA TGAACTTATG GAGGAAAAAG TAGCTTTCTA CATAAGAGAC 
ACAATGGAAG CCTTGCAGTA TCTTCACAAT TGCCGAGTGG CTCATTTAGA TATAAAGCCA 
GAGAATCTGC TCATCGATTT AAGGATCCCA GTTCCTCGTG TCAAGATCAT TGATTTGGAA 
GATGCAGTTC AAATCACAGG CCATTACCAC GTCCACCATC TCCTTGGAAA CCCAGAGTTT 
GCAGCCCCTG AAGTTATCCA GGGCCTCCCC GTGTCCCTGA GCACAGACAT CTGGAGCATC 
GGGGTCCTCA CCTACGTCAT GCTGAGCGGT GTCTCCCCGT TCCTGGATGA AAGCAAAGAG 
GAGACGTGTA TCAATGTGTG CAGAGTCGAT TTCAGCTTCC CGCCCGAGTA CTTCTCTGAT 
GTGAGCCATG CTGCTAGGGA TTTCATCAAC GTCATCCTCC AGGAAGACTC CAGGAGGAGG 
CCGACAGCAG CCACTTGTTT ACAGCACCCA TGGCTGCAGC CACACAATGG CAGCTACTCC 
AAAATCCCGC TGGATACCTC CCGCTTGGCA TCCTTCATTG AGCGCCGCAG ACACCAGTAC 
GACGTGCACC CCGTCCCCGG TGTCAAGAGT TTCCTGATGA GCAGGCTGAA CCCCGGGACG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145462.1
CDS	244..9186
Translation	MNPTEGAAEEGAVPDSEVDAFFRTGSFRNDGLKAADVLPILKEKVAFVSGGRDKRGGPVLTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLAKLVDPSQLTDEFEGSLDYNHDEWIELRVSLEEFFNSAIHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVIKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKELLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAVSNNKTPHNSSISHIESVLQQLDEAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDIDLATQVQELLEFLHEKQHELELNADQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEVLLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEIDHVIPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPGVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHNSTGESAEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKRYRDFSLRMGKYRYSLEKALGINTEDNKDLELDIIPASLSDREVKLRDANHEVNEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPAGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSSQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDSFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASSIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKQRNNSRRDGVDDADSQGDGSSQPDTISIASRTSQNTVDSDKHFVYGRDTGQLSGGCELTVVLQDFTAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPSGKEAYSVSSNENGGKSDSVANLQPQPSLNSIQSSPGPKRSTNTLKKWLTSPVRRLNSGKAESNIKKQKKVRDGRKSFDLGSPKTGDETTPQGDSADESKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSSLLAARQSSSDVPTAADLVSAIEQLVKSKLTLEGGSYRGSLKDATGCLNEGMAPSTPPRNLEEEQKAKAMRGRMFVLNELVQTEKDYVKDLGTVVEGFMKRIEEKGVSEDMKGKDKIVFGNIHQIYDWHKDFFLAELEKCLQEPDRLAQLFIKHERRLHMYVVYCQNKPRSEYIVAEYEAYFEEVQQEINQRLTISDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSEIEKAVELMCLVPKRCNDMMNLGRLQGFEGKLTAQGKLLQQDTFYVTEHDSGVQSRPKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVIEENVDGDPCKFALMNRETSEKVVLQAANPEIQQAWVQDINQVLDTQRDFLNALQSPIEYQRKESSTAVLRPQAGRVAQPNSRPYSSVPVGSEKPLKATSRNPSLPPLKISTSNGSTGYEQPGDKYEQSKTDLGGCNGTSSMVVIKDYYAVKEDEICVNQGEVVQILAINQQNMFLVYQPANDHSPAAEGWIPGNILAPLTKPTTDNSDGSIKKSCSWHTLRMRKRAEKESSGKNEANGPRKSKDILGNKVSVKETNSSEESECDDLDPNTSMEIINPNFIQEVAPEFLVLLVDITCLLGDTVMLQCKVCGRPKPTITWKGPDQNILDNDNSTATYTVSSCDSGELMLKICNVMPQDSGIYTCVATNEHGTASTSATIKVQGVPAAPNRPIAQERSCTSVILRWLPPSSTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCQYQFRVSASNPWGISLPSDPSEFVRLPEYDSAADGATISWKENFDLAYAELHEIGRGRFSIVKKCVHKATRKDVAVKFISKKMKKKEQAAHEAALLQHLQHPQYITIHDTYESPTSYILVLELMDDGRLLDYLMNHDELMEEKVAFYIRDTMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKIIDLEDAVQITGHYHVHHLLGNPEFAAPEVIQGLPVSLSTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPPEYFSDVSHAARDFINVILQEDSRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLASFIERRRHQYDVHPVPGVKSFLMSRLNPGT

Target ORF information:

RefSeq Version	XM_015289976.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289976.2

ORF Insert Sequence:

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGCATT TCGTCTACGG CAGAGACACA 
GGACAGTTGT CTGGAGGGTG TGAACTAACC GTGGTGCTCC AGGACTTCAC GGCGGGCCAC 
AGCAGCGAGC TGAGCATTCA GGTGGGGCAG ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA 
AGGCCAGGCT GGTGCTTGGT GCGGACCACG GAGCGGAGCC CACCTCAGGA AGGCCTGGTC 
CCCAGCAGCG CTCTCTGCAT TTCCCATTCC CGCAGCAGCG TGGAGATGGA TTGCTTCTTC 
CCTTCAGGAA AAGAGGCATA TTCAGTCTCT TCCAATGAAA ACGGGGGCAA GTCCGATTCA 
GTGGCCAACT TACAGCCCCA GCCATCCCTC AACTCCATCC AGAGCTCTCC CGGCCCCAAG 
CGCTCGACCA ACACGCTGAA GAAATGGCTG ACGAGCCCCG TGCGCCGGCT GAACAGCGGG 
AAGGCCGAGA GCAACATCAA GAAGCAAAAG AAGGTGCGAG ATGGCAGGAA GAGCTTTGAC 
CTCGGCTCGC CGAAGACTGG AGATGAAACC ACTCCTCAAG GAGACAGCGC GGATGAGAGC 
AAGAAGGGTT GGGGAGAAGA TGAGCCAGAT GAAGAGTCTC ACACACCTCT TCCTCCTCCT 
ATGAAGATTT TTGACAATGA TCCAACCCAA GATGAGATGT CCTCTTCTTT GCTAGCTGCT 
CGGCAGAGCT CCTCCGATGT CCCAACTGCT GCGGATCTTG TCAGTGCAAT AGAACAGTTG 
GTCAAAAGTA AGCTGACCCT TGAAGGAGGA TCTTACCGAG GAAGCTTAAA AGATGCCACA 
GGCTGCTTGA ATGAAGGAAT GGCGCCTTCA ACTCCCCCAA GAAACCTTGA AGAGGAACAA 
AAAGCCAAAG CAATGAGAGG CAGGATGTTT GTCTTAAATG AACTGGTCCA GACTGAAAAA 
GACTACGTCA AAGACTTGGG AACCGTTGTG GAGGGCTTCA TGAAGAGAAT AGAAGAAAAG 
GGAGTTTCCG AAGATATGAA AGGGAAAGAC AAGATAGTGT TTGGCAATAT TCACCAGATC 
TATGACTGGC ACAAAGACTT TTTCCTGGCT GAACTGGAAA AATGTCTTCA GGAGCCTGAC 
CGTTTAGCAC AGCTTTTTAT TAAACATGAG CGGCGATTAC ACATGTATGT GGTGTACTGC 
CAAAACAAGC CACGATCTGA ATATATAGTA GCTGAATACG AGGCATATTT TGAGGAGGTT 
CAACAGGAAA TAAACCAACG GCTGACCATC AGTGACTTTC TGATCAAACC CATTCAGAGA 
ATAACTAAAT ATCAGCTTCT TCTCAAGGAC TTCCTGAGGT ACAGTGAAAA AGCTGGTCTG 
GAGTGCTCTG AAATAGAGAA AGCAGTTGAG TTAATGTGCC TTGTACCAAA ACGTTGCAAT 
GATATGATGA ACCTGGGTCG CCTCCAAGGC TTTGAGGGTA AGCTGACAGC CCAGGGCAAG 
CTGCTGCAGC AGGACACCTT CTACGTGACA GAGCATGACT CCGGAGTTCA GTCCCGACCA 
AAGGAACGTA GGGTGTTTCT CTTTGAGCAA ATAGTTATCT TCAGCGAGCT GCTTAGGAAA 
GGGTCTTTAA CACCAGGCTA CATGTTCAAG AGAAGCATAA AGATGAACTA CCTTGTCATT 
GAAGAAAATG TGGATGGTGA TCCCTGCAAG TTTGCTCTAA TGAATCGGGA AACATCTGAG 
AAAGTTGTTT TACAGGCAGC TAATCCAGAA ATTCAGCAGG CTTGGGTACA GGACATCAAC 
CAGGTCCTGG ACACACAAAG GGATTTCCTA AATGCACTGC AGTCTCCCAT AGAGTACCAA 
CGCAAAGAAA GTAGCACAGC AGTGCTGAGG CCCCAGGCTG GTAGGGTCGC TCAGCCCAAC 
AGCAGACCCT ATTCTTCTGT TCCAGTAGGG TCTGAGAAGC CTTTGAAGGC TACAAGCCGT 
AACCCATCTC TCCCACCCCT GAAGATATCT ACCTCCAACG GCAGTACAGG GTATGAACAG 
CCCGGAGACA AGTATGAACA AAGCAAGACT GATTTGGGAG GGTGCAATGG GACATCTTCC 
ATGGTGGTGA TTAAAGATTA CTATGCAGTG AAGGAGGACG AGATCTGTGT GAATCAAGGA 
GAGGTGGTTC AGATCCTTGC TATCAACCAG CAAAACATGT TCCTGGTGTA CCAGCCTGCA 
AATGACCACT CTCCAGCTGC TGAAGGATGG ATCCCTGGCA ATATCTTGGC TCCCCTCACT 
AAACCCACTA CAGATAATAG CGACGGAAGC ATCAAGAAGT CATGTTCATG GCATACCCTG 
CGCATGAGAA AGCGGGCGGA AAAGGAGAGC AGTGGCAAAA ATGAAGCCAA TGGGCCACGT 
AAATCCAAGG ATATTCTGGG GAACAAAGTC TCTGTTAAAG AGACAAACAG CTCAGAGGAA 
TCAGAGTGTG ATGACCTTGA CCCCAATACT AGCATGGAGA TAATCAACCC CAATTTCATT 
CAGGAAGTGG CTCCAGAGTT CCTTGTGCTG TTGGTGGATA TCACCTGCCT GCTTGGCGAC 
ACTGTGATGC TGCAGTGCAA AGTCTGCGGC CGGCCCAAAC CAACAATAAC CTGGAAAGGA 
CCTGATCAAA ACATTCTTGA CAATGACAAC AGCACGGCCA CATACACGGT GTCATCCTGT 
GACTCTGGAG AGCTCATGCT GAAGATCTGC AATGTGATGC CTCAGGACAG TGGCATTTAC 
ACTTGTGTGG CAACTAACGA GCATGGAACG GCATCAACCT CAGCCACAAT CAAAGTGCAA 
GGTGTACCGG CTGCCCCAAA TCGCCCCATT GCTCAGGAGA GAAGCTGCAC CTCTGTGATC 
CTTCGCTGGC TGCCTCCATC CAGTACAGGC AATTGCACCA TTTCTGGCTA CACAGTGGAG 
TACAGAGAAG AAGGCTCACA GGTCTGGCAG CAGTCAGTGG CCTCCACACT GGACACTTAC 
CTTGTCATTG AAGACCTGTC CCCAGGCTGC CAGTATCAGT TCCGTGTGAG TGCCAGCAAC 
CCCTGGGGGA TCAGCTTGCC CAGTGACCCA TCTGAATTCG TGAGGCTTCC AGAGTATGAT 
TCTGCTGCTG ATGGTGCCAC TATTTCGTGG AAGGAAAACT TTGATCTCGC TTATGCGGAG 
CTGCATGAGA TTGGGAGGGG ACGATTCTCC ATTGTAAAGA AATGCGTTCA CAAAGCTACC 
CGGAAAGACG TGGCTGTAAA ATTCATCAGT AAAAAAATGA AGAAGAAGGA GCAAGCAGCC 
CACGAGGCAG CTCTGTTACA GCACTTACAG CATCCTCAGT ACATCACTAT CCATGATACC 
TATGAGTCTC CTACTTCTTA CATCCTAGTC CTGGAGCTGA TGGATGATGG TCGTCTCCTA 
GATTATCTAA TGAACCATGA TGAACTTATG GAGGAAAAAG TAGCTTTCTA CATAAGAGAC 
ACAATGGAAG CCTTGCAGTA TCTTCACAAT TGCCGAGTGG CTCATTTAGA TATAAAGCCA 
GAGAATCTGC TCATCGATTT AAGGATCCCA GTTCCTCGTG TCAAGATCAT TGATTTGGAA 
GATGCAGTTC AAATCACAGG CCATTACCAC GTCCACCATC TCCTTGGAAA CCCAGAGTTT 
GCAGCCCCTG AAGTTATCCA GGGCCTCCCC GTGTCCCTGA GCACAGACAT CTGGAGCATC 
GGGGTCCTCA CCTACGTCAT GCTGAGCGGT GTCTCCCCGT TCCTGGATGA AAGCAAAGAG 
GAGACGTGTA TCAATGTGTG CAGAGTCGAT TTCAGCTTCC CGCCCGAGTA CTTCTCTGAT 
GTGAGCCATG CTGCTAGGGA TTTCATCAAC GTCATCCTCC AGGAAGACTC CAGGAGGAGG 
CCGACAGCAG CCACTTGTTT ACAGCACCCA TGGCTGCAGC CACACAATGG CAGCTACTCC 
AAAATCCCGC TGGATACCTC CCGCTTGGCA TCCTTCATTG AGCGCCGCAG ACACCAGTAC 
GACGTGCACC CCGTCCCCGG TGTCAAGAGT TTCCTGATGA GCAGGCTGAA CCCCGGGACG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KALRN ( XM_015289977.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145463.1 Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X3, mRNA.

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CloneID	OGa38673
Clone ID Related Accession (Same CDS sequence)	XM_015289977.1 , XM_015289977.2
Accession Version	XM_015289977.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	kalirin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG AAGACACACC AGAGTGCCCT GCAGGTCCAG 
CAGAAAGCCG AAGTGCTGCT GCAGGCTGGG CATTATGATG CTGATGCCAT CAGAGAATGC 
GCTGAAAAGG TGGCCCTGCA CTGGCAGCAG CTGATGCTGA AGATGGAGGA CAGGCTCAAG 
CTGGTCAATG CCTCGGTGGC CTTCTACAAA ACCTCTGAGC AGGTGTGCAG TGTATTAGAG 
AGCCTGGAGC AAGAATACAG ACGTGATGAA GACTGGTGTG GAGGTCGAGA TAAACTTGGA 
CCAGCAGCTG AGATAGACCA CGTCATCCCT CTGATCAGCA AGCATCTGGA GCAGAAGGAG 
GCTTTTCTCA AGGCCTGCAC ACTGGCTCGA AGGAATGCTG AGGTATTCCT CAAGTACATC 
CACAGGAACA ATGTCAGCAT GCCCGGAGTC GCAAGCCATA CACGGGGACC TGAGCAGCAA 
GTGAAAGCCA TCCTGAGTGA GTTGCTGCAG AGGGAGAACC GGGTCCTTCA CTTCTGGACC 
CTGAAGAAGC GGAGGTTAGA TCAGTGCCAG CAGTACGTTG TATTTGAGCG CAGTGCCAAG 
CAGGCCCTGG ACTGGATCCA AGAGACAGGC GAATATTACC TCTCTACTCA CAACTCTACA 
GGGGAATCAG CAGAAGAAAC TCAGGAACTC CTGAAGGAAT ATGGGGAATT CAGAGTACCT 
GCGAAGCAAA CAAAGGAGAA AGTGAAGCTC CTCATCCAGC TGGCTGACAG CTTTGTAGAA 
AAAGGACATA TACACGCCAC TGAAATCAGG AAGTGGGTCA CCACCGTTGA CAAACGCTAC 
CGTGACTTCT CTCTCAGGAT GGGGAAATAC CGCTACTCCC TGGAGAAAGC CCTTGGAATC 
AATACAGAGG ATAACAAGGA CCTCGAACTA GATATTATTC CAGCAAGTCT TTCAGACCGG 
GAGGTAAAGC TGCGAGATGC AAATCACGAA GTCAATGAAG AAAAAAGAAA GTCTGCTAGA 
AAGAAAGAAT TCATTATGGC CGAGCTTCTT CAGACAGAGA AAGCTTATGT CAGGGACTTA 
CATGAATGTT TAGAGACTTA CCTTTGGGAA ATGACAAGTG GGGTGGAAGA AATACCCGCT 
GGGATCCTTA ATAAAGAACA CATCATCTTT GGCAATATTC AGGAGATATA TGACTTCCAT 
AACAACATTT TCCTGAAAGA ACTCGAGAAG TATGAGCAAC TGCCTGAGGA TGTGGGTCAC 
TGCTTTGTCA CCTGGGCAGA TAAATTTCAG ATGTATGTCA CCTACTGCAA AAACAAGCCT 
GACTCCAGCC AGCTCATCCT GGAACACGCA GGGACTTTCT TTGATGAAAT TCAGCAAAGG 
CATGGTCTGG CCAACTCTAT CTCTTCTTAT TTAATCAAGC CTGTCCAGAG GATCACAAAA 
TATCAACTCC TCCTGAAGGA ACTGCTTACC TGCTGTGAGG AAGGCAAAGG GGAACTCAAG 
GATGGTCTGG AAGTGATGCT CAGTGTCCCC AAGAAAGCCA ATGATGCAAT GCACGTCAGC 
ATGCTGGAAG GGTTTGATGA GAATCTAGAC GTCCAGGGAG AGCTGATTCT TCAGGATTCC 
TTCCAAGTGT GGGACCCCAA GTCTCTCATT CGGAAAGGCC GTGAGAGACA TTTGTTTCTC 
TTTGAAATCT CTTTGGTGTT TAGCAAAGAG ATCAAAGACT CTTCAGGACA CACAAAATAT 
GTTTACAAGA ACAAGTTACT GACCTCAGAA CTGGGAGTGA CTGAACATGT TGAAGGAGAT 
CCCTGTAAAT TTGCCTTGTG GTCTGGACGA ACACCATCCT CAGACAATAA AACAGTGCTG 
AAAGCATCGA GTATTGAAAC AAAACAGGAG TGGATCAAAA ATATCCGAGA AGTCATCCAA 
GAAAGAATTA TCCATCTGAA AGGAGCTCTG AAAGAGCCAA TTCAGCTCCC CAAGACACCA 
GCAAAGCAGC GAAACAACAG TAGAAGAGAT GGAGTAGATG ATGCAGACAG CCAAGGAGAT 
GGAAGCAGTC AACCTGACAC CATTTCCATT GCATCCAGGA CATCACAGAA TACAGTGGAC 
AGTGATAAGC ATTTCGTCTA CGGCAGAGAC ACAGGACAGT TGTCTGGAGG GTGTGAACTA 
ACCGTGGTGC TCCAGGACTT CACGGCGGGC CACAGCAGCG AGCTGAGCAT TCAGGTGGGG 
CAGACAGTGG AGCTGCTGGA GAGGCCAAGT GAAAGGCCAG GCTGGTGCTT GGTGCGGACC 
ACGGAGCGGA GCCCACCTCA GGAAGGCCTG GTCCCCAGCA GCGCTCTCTG CATTTCCCAT 
TCCCGCAGCA GCGTGGAGAT GGATTGCTTC TTCCCTTCAG GAAAAGAGGC ATATTCAGTC 
TCTTCCAATG AAAACGGGGG CAAGTCCGAT TCAGTGGCCA ACTTACAGCC CCAGCCATCC 
CTCAACTCCA TCCAGAGCTC TCCCGGCCCC AAGCGCTCGA CCAACACGCT GAAGAAATGG 
CTGACGAGCC CCGTGCGCCG GCTGAACAGC GGGAAGGCCG AGAGCAACAT CAAGAAGCAA 
AAGAAGGTGC GAGATGGCAG GAAGAGCTTT GACCTCGGCT CGCCGAAGAC TGGAGATGAA 
ACCACTCCTC AAGGAGACAG CGCGGATGAG AAGAGCAAGA AGGGTTGGGG AGAAGATGAG 
CCAGATGAAG AGTCTCACAC ACCTCTTCCT CCTCCTATGA AGATTTTTGA CAATGATCCA 
ACCCAAGATG AGATGTCCTC TTCTTTGCTA GCTGCTCGGC AGAGCTCCTC CGATGTCCCA 
ACTGCTGCGG ATCTTGTCAG TGCAATAGAA CAGTTGGTCA AAAGTAAGCT GACCCTTGAA 
GGAGGATCTT ACCGAGGAAG CTTAAAAGAT GCCACAGGCT GCTTGAATGA AGGAATGGCG 
CCTTCAACTC CCCCAAGAAA CCTTGAAGAG GAACAAAAAG CCAAAGCAAT GAGAGGCAGG 
ATGTTTGTCT TAAATGAACT GGTCCAGACT GAAAAAGACT ACGTCAAAGA CTTGGGAACC 
GTTGTGGAGG GCTTCATGAA GAGAATAGAA GAAAAGGGAG TTTCCGAAGA TATGAAAGGG 
AAAGACAAGA TAGTGTTTGG CAATATTCAC CAGATCTATG ACTGGCACAA AGACTTTTTC 
CTGGCTGAAC TGGAAAAATG TCTTCAGGAG CCTGACCGTT TAGCACAGCT TTTTATTAAA 
CATGAGCGGC GATTACACAT GTATGTGGTG TACTGCCAAA ACAAGCCACG ATCTGAATAT 
ATAGTAGCTG AATACGAGGC ATATTTTGAG GAGGTTCAAC AGGAAATAAA CCAACGGCTG 
ACCATCAGTG ACTTTCTGAT CAAACCCATT CAGAGAATAA CTAAATATCA GCTTCTTCTC 
AAGGACTTCC TGAGGTACAG TGAAAAAGCT GGTCTGGAGT GCTCTGAAAT AGAGAAAGCA 
GTTGAGTTAA TGTGCCTTGT ACCAAAACGT TGCAATGATA TGATGAACCT GGGTCGCCTC 
CAAGGCTTTG AGGGTAAGCT GACAGCCCAG GGCAAGCTGC TGCAGCAGGA CACCTTCTAC 
GTGACAGAGC ATGACTCCGG AGTTCAGTCC CGACCAAAGG AACGTAGGGT GTTTCTCTTT 
GAGCAAATAG TTATCTTCAG CGAGCTGCTT AGGAAAGGGT CTTTAACACC AGGCTACATG 
TTCAAGAGAA GCATAAAGAT GAACTACCTT GTCATTGAAG AAAATGTGGA TGGTGATCCC 
TGCAAGTTTG CTCTAATGAA TCGGGAAACA TCTGAGAAAG TTGTTTTACA GGCAGCTAAT 
CCAGAAATTC AGCAGGCTTG GGTACAGGAC ATCAACCAGG TCCTGGACAC ACAAAGGGAT 
TTCCTAAATG CACTGCAGTC TCCCATAGAG TACCAACGCA AAGAAAGTAG CACAGCAGTG 
CTGAGGCCCC AGGCTGGTAG GGTCGCTCAG CCCAACAGCA GACCCTATTC TTCTGTTCCA 
GTAGGGTCTG AGAAGCCTTT GAAGGCTACA AGCCGTAACC CATCTCTCCC ACCCCTGAAG 
ATATCTACCT CCAACGGCAG TACAGGGTAT GAACAGCCCG GAGACAAGTA TGAACAAAGC 
AAGACTGATT TGGGAGGGTG CAATGGGACA TCTTCCATGG TGGTGATTAA AGATTACTAT 
GCAGTGAAGG AGGACGAGAT CTGTGTGAAT CAAGGAGAGG TGGTTCAGAT CCTTGCTATC 
AACCAGCAAA ACATGTTCCT GGTGTACCAG CCTGCAAATG ACCACTCTCC AGCTGCTGAA 
GGATGGATCC CTGGCAATAT CTTGGCTCCC CTCACTAAAC CCACTACAGA TAATAGCGAC 
GGAAGCATCA AGAAGTCATG TTCATGGCAT ACCCTGCGCA TGAGAAAGCG GGCGGAAAAG 
GAGAGCAGTG GCAAAAATGA AGCCAATGGG CCACGTAAAT CCAAGGATAT TCTGGGGAAC 
AAAGTCTCTG TTAAAGAGAC AAACAGCTCA GAGGAATCAG AGTGTGATGA CCTTGACCCC 
AATACTAGCA TGGAGATAAT CAACCCCAAT TTCATTCAGG AAGTGGCTCC AGAGTTCCTT 
GTGCTGTTGG TGGATATCAC CTGCCTGCTT GGCGACACTG TGATGCTGCA GTGCAAAGTC 
TGCGGCCGGC CCAAACCAAC AATAACCTGG AAAGGACCTG ATCAAAACAT TCTTGACAAT 
GACAACAGCA CGGCCACATA CACGGTGTCA TCCTGTGACT CTGGAGAGCT CATGCTGAAG 
ATCTGCAATG TGATGCCTCA GGACAGTGGC ATTTACACTT GTGTGGCAAC TAACGAGCAT 
GGAACGGCAT CAACCTCAGC CACAATCAAA GTGCAAGGTG TACCGGCTGC CCCAAATCGC 
CCCATTGCTC AGGAGAGAAG CTGCACCTCT GTGATCCTTC GCTGGCTGCC TCCATCCAGT 
ACAGGCAATT GCACCATTTC TGGCTACACA GTGGAGTACA GAGAAGAAGG CTCACAGGTC 
TGGCAGCAGT CAGTGGCCTC CACACTGGAC ACTTACCTTG TCATTGAAGA CCTGTCCCCA 
GGCTGCCAGT ATCAGTTCCG TGTGAGTGCC AGCAACCCCT GGGGGATCAG CTTGCCCAGT 
GACCCATCTG AATTCGTGAG GCTTCCAGAG TATGATTCTG CTGCTGATGG TGCCACTATT 
TCGTGGAAGG AAAACTTTGA TCTCGCTTAT GCGGAGCTGC ATGAGATTGG GAGGGGACGA 
TTCTCCATTG TAAAGAAATG CGTTCACAAA GCTACCCGGA AAGACGTGGC TGTAAAATTC 
ATCAGTAAAA AAATGAAGAA GAAGGAGCAA GCAGCCCACG AGGCAGCTCT GTTACAGCAC 
TTACAGCATC CTCAGTACAT CACTATCCAT GATACCTATG AGTCTCCTAC TTCTTACATC 
CTAGTCCTGG AGCTGATGGA TGATGGTCGT CTCCTAGATT ATCTAATGAA CCATGATGAA 
CTTATGGAGG AAAAAGTAGC TTTCTACATA AGAGACACAA TGGAAGCCTT GCAGTATCTT 
CACAATTGCC GAGTGGCTCA TTTAGATATA AAGCCAGAGA ATCTGCTCAT CGATTTAAGG 
ATCCCAGTTC CTCGTGTCAA GATCATTGAT TTGGAAGATG CAGTTCAAAT CACAGGCCAT 
TACCACGTCC ACCATCTCCT TGGAAACCCA GAGTTTGCAG CCCCTGAAGT TATCCAGGGC 
CTCCCCGTGT CCCTGAGCAC AGACATCTGG AGCATCGGGG TCCTCACCTA CGTCATGCTG 
AGCGGTGTCT CCCCGTTCCT GGATGAAAGC AAAGAGGAGA CGTGTATCAA TGTGTGCAGA 
GTCGATTTCA GCTTCCCGCC CGAGTACTTC TCTGATGTGA GCCATGCTGC TAGGGATTTC 
ATCAACGTCA TCCTCCAGGA AGACTCCAGG AGGAGGCCGA CAGCAGCCAC TTGTTTACAG 
CACCCATGGC TGCAGCCACA CAATGGCAGC TACTCCAAAA TCCCGCTGGA TACCTCCCGC 
TTGGCATCCT TCATTGAGCG CCGCAGACAC CAGTACGACG TGCACCCCGT CCCCGGTGTC 
AAGAGTTTCC TGATGAGCAG GCTGAACCCC GGGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145463.1
CDS	234..9152
Translation	MNPTEGAAEEGAVPDSEVDAFFRTGSFRNDGLKAADVLPILKEKVAFVSGGRDKRGGPVLTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLAKLVDPSQLTDEFEGSLDYNHDEWIELRVSLEEFFNSAIHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVIKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKELLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAVSNNKTPHNSSISHIESVLQQLDEAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDIDLATQVQELLEFLHEKQHELELNADQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIEKTHQSALQVQQKAEVLLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEIDHVIPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPGVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHNSTGESAEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKRYRDFSLRMGKYRYSLEKALGINTEDNKDLELDIIPASLSDREVKLRDANHEVNEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPAGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSSQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDSFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASSIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKQRNNSRRDGVDDADSQGDGSSQPDTISIASRTSQNTVDSDKHFVYGRDTGQLSGGCELTVVLQDFTAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPSGKEAYSVSSNENGGKSDSVANLQPQPSLNSIQSSPGPKRSTNTLKKWLTSPVRRLNSGKAESNIKKQKKVRDGRKSFDLGSPKTGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSSLLAARQSSSDVPTAADLVSAIEQLVKSKLTLEGGSYRGSLKDATGCLNEGMAPSTPPRNLEEEQKAKAMRGRMFVLNELVQTEKDYVKDLGTVVEGFMKRIEEKGVSEDMKGKDKIVFGNIHQIYDWHKDFFLAELEKCLQEPDRLAQLFIKHERRLHMYVVYCQNKPRSEYIVAEYEAYFEEVQQEINQRLTISDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSEIEKAVELMCLVPKRCNDMMNLGRLQGFEGKLTAQGKLLQQDTFYVTEHDSGVQSRPKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVIEENVDGDPCKFALMNRETSEKVVLQAANPEIQQAWVQDINQVLDTQRDFLNALQSPIEYQRKESSTAVLRPQAGRVAQPNSRPYSSVPVGSEKPLKATSRNPSLPPLKISTSNGSTGYEQPGDKYEQSKTDLGGCNGTSSMVVIKDYYAVKEDEICVNQGEVVQILAINQQNMFLVYQPANDHSPAAEGWIPGNILAPLTKPTTDNSDGSIKKSCSWHTLRMRKRAEKESSGKNEANGPRKSKDILGNKVSVKETNSSEESECDDLDPNTSMEIINPNFIQEVAPEFLVLLVDITCLLGDTVMLQCKVCGRPKPTITWKGPDQNILDNDNSTATYTVSSCDSGELMLKICNVMPQDSGIYTCVATNEHGTASTSATIKVQGVPAAPNRPIAQERSCTSVILRWLPPSSTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCQYQFRVSASNPWGISLPSDPSEFVRLPEYDSAADGATISWKENFDLAYAELHEIGRGRFSIVKKCVHKATRKDVAVKFISKKMKKKEQAAHEAALLQHLQHPQYITIHDTYESPTSYILVLELMDDGRLLDYLMNHDELMEEKVAFYIRDTMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKIIDLEDAVQITGHYHVHHLLGNPEFAAPEVIQGLPVSLSTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPPEYFSDVSHAARDFINVILQEDSRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLASFIERRRHQYDVHPVPGVKSFLMSRLNPGT

Target ORF information:

RefSeq Version	XM_015289977.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289977.1

ORF Insert Sequence:

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG AAGACACACC AGAGTGCCCT GCAGGTCCAG 
CAGAAAGCCG AAGTGCTGCT GCAGGCTGGG CATTATGATG CTGATGCCAT CAGAGAATGC 
GCTGAAAAGG TGGCCCTGCA CTGGCAGCAG CTGATGCTGA AGATGGAGGA CAGGCTCAAG 
CTGGTCAATG CCTCGGTGGC CTTCTACAAA ACCTCTGAGC AGGTGTGCAG TGTATTAGAG 
AGCCTGGAGC AAGAATACAG ACGTGATGAA GACTGGTGTG GAGGTCGAGA TAAACTTGGA 
CCAGCAGCTG AGATAGACCA CGTCATCCCT CTGATCAGCA AGCATCTGGA GCAGAAGGAG 
GCTTTTCTCA AGGCCTGCAC ACTGGCTCGA AGGAATGCTG AGGTATTCCT CAAGTACATC 
CACAGGAACA ATGTCAGCAT GCCCGGAGTC GCAAGCCATA CACGGGGACC TGAGCAGCAA 
GTGAAAGCCA TCCTGAGTGA GTTGCTGCAG AGGGAGAACC GGGTCCTTCA CTTCTGGACC 
CTGAAGAAGC GGAGGTTAGA TCAGTGCCAG CAGTACGTTG TATTTGAGCG CAGTGCCAAG 
CAGGCCCTGG ACTGGATCCA AGAGACAGGC GAATATTACC TCTCTACTCA CAACTCTACA 
GGGGAATCAG CAGAAGAAAC TCAGGAACTC CTGAAGGAAT ATGGGGAATT CAGAGTACCT 
GCGAAGCAAA CAAAGGAGAA AGTGAAGCTC CTCATCCAGC TGGCTGACAG CTTTGTAGAA 
AAAGGACATA TACACGCCAC TGAAATCAGG AAGTGGGTCA CCACCGTTGA CAAACGCTAC 
CGTGACTTCT CTCTCAGGAT GGGGAAATAC CGCTACTCCC TGGAGAAAGC CCTTGGAATC 
AATACAGAGG ATAACAAGGA CCTCGAACTA GATATTATTC CAGCAAGTCT TTCAGACCGG 
GAGGTAAAGC TGCGAGATGC AAATCACGAA GTCAATGAAG AAAAAAGAAA GTCTGCTAGA 
AAGAAAGAAT TCATTATGGC CGAGCTTCTT CAGACAGAGA AAGCTTATGT CAGGGACTTA 
CATGAATGTT TAGAGACTTA CCTTTGGGAA ATGACAAGTG GGGTGGAAGA AATACCCGCT 
GGGATCCTTA ATAAAGAACA CATCATCTTT GGCAATATTC AGGAGATATA TGACTTCCAT 
AACAACATTT TCCTGAAAGA ACTCGAGAAG TATGAGCAAC TGCCTGAGGA TGTGGGTCAC 
TGCTTTGTCA CCTGGGCAGA TAAATTTCAG ATGTATGTCA CCTACTGCAA AAACAAGCCT 
GACTCCAGCC AGCTCATCCT GGAACACGCA GGGACTTTCT TTGATGAAAT TCAGCAAAGG 
CATGGTCTGG CCAACTCTAT CTCTTCTTAT TTAATCAAGC CTGTCCAGAG GATCACAAAA 
TATCAACTCC TCCTGAAGGA ACTGCTTACC TGCTGTGAGG AAGGCAAAGG GGAACTCAAG 
GATGGTCTGG AAGTGATGCT CAGTGTCCCC AAGAAAGCCA ATGATGCAAT GCACGTCAGC 
ATGCTGGAAG GGTTTGATGA GAATCTAGAC GTCCAGGGAG AGCTGATTCT TCAGGATTCC 
TTCCAAGTGT GGGACCCCAA GTCTCTCATT CGGAAAGGCC GTGAGAGACA TTTGTTTCTC 
TTTGAAATCT CTTTGGTGTT TAGCAAAGAG ATCAAAGACT CTTCAGGACA CACAAAATAT 
GTTTACAAGA ACAAGTTACT GACCTCAGAA CTGGGAGTGA CTGAACATGT TGAAGGAGAT 
CCCTGTAAAT TTGCCTTGTG GTCTGGACGA ACACCATCCT CAGACAATAA AACAGTGCTG 
AAAGCATCGA GTATTGAAAC AAAACAGGAG TGGATCAAAA ATATCCGAGA AGTCATCCAA 
GAAAGAATTA TCCATCTGAA AGGAGCTCTG AAAGAGCCAA TTCAGCTCCC CAAGACACCA 
GCAAAGCAGC GAAACAACAG TAGAAGAGAT GGAGTAGATG ATGCAGACAG CCAAGGAGAT 
GGAAGCAGTC AACCTGACAC CATTTCCATT GCATCCAGGA CATCACAGAA TACAGTGGAC 
AGTGATAAGC ATTTCGTCTA CGGCAGAGAC ACAGGACAGT TGTCTGGAGG GTGTGAACTA 
ACCGTGGTGC TCCAGGACTT CACGGCGGGC CACAGCAGCG AGCTGAGCAT TCAGGTGGGG 
CAGACAGTGG AGCTGCTGGA GAGGCCAAGT GAAAGGCCAG GCTGGTGCTT GGTGCGGACC 
ACGGAGCGGA GCCCACCTCA GGAAGGCCTG GTCCCCAGCA GCGCTCTCTG CATTTCCCAT 
TCCCGCAGCA GCGTGGAGAT GGATTGCTTC TTCCCTTCAG GAAAAGAGGC ATATTCAGTC 
TCTTCCAATG AAAACGGGGG CAAGTCCGAT TCAGTGGCCA ACTTACAGCC CCAGCCATCC 
CTCAACTCCA TCCAGAGCTC TCCCGGCCCC AAGCGCTCGA CCAACACGCT GAAGAAATGG 
CTGACGAGCC CCGTGCGCCG GCTGAACAGC GGGAAGGCCG AGAGCAACAT CAAGAAGCAA 
AAGAAGGTGC GAGATGGCAG GAAGAGCTTT GACCTCGGCT CGCCGAAGAC TGGAGATGAA 
ACCACTCCTC AAGGAGACAG CGCGGATGAG AAGAGCAAGA AGGGTTGGGG AGAAGATGAG 
CCAGATGAAG AGTCTCACAC ACCTCTTCCT CCTCCTATGA AGATTTTTGA CAATGATCCA 
ACCCAAGATG AGATGTCCTC TTCTTTGCTA GCTGCTCGGC AGAGCTCCTC CGATGTCCCA 
ACTGCTGCGG ATCTTGTCAG TGCAATAGAA CAGTTGGTCA AAAGTAAGCT GACCCTTGAA 
GGAGGATCTT ACCGAGGAAG CTTAAAAGAT GCCACAGGCT GCTTGAATGA AGGAATGGCG 
CCTTCAACTC CCCCAAGAAA CCTTGAAGAG GAACAAAAAG CCAAAGCAAT GAGAGGCAGG 
ATGTTTGTCT TAAATGAACT GGTCCAGACT GAAAAAGACT ACGTCAAAGA CTTGGGAACC 
GTTGTGGAGG GCTTCATGAA GAGAATAGAA GAAAAGGGAG TTTCCGAAGA TATGAAAGGG 
AAAGACAAGA TAGTGTTTGG CAATATTCAC CAGATCTATG ACTGGCACAA AGACTTTTTC 
CTGGCTGAAC TGGAAAAATG TCTTCAGGAG CCTGACCGTT TAGCACAGCT TTTTATTAAA 
CATGAGCGGC GATTACACAT GTATGTGGTG TACTGCCAAA ACAAGCCACG ATCTGAATAT 
ATAGTAGCTG AATACGAGGC ATATTTTGAG GAGGTTCAAC AGGAAATAAA CCAACGGCTG 
ACCATCAGTG ACTTTCTGAT CAAACCCATT CAGAGAATAA CTAAATATCA GCTTCTTCTC 
AAGGACTTCC TGAGGTACAG TGAAAAAGCT GGTCTGGAGT GCTCTGAAAT AGAGAAAGCA 
GTTGAGTTAA TGTGCCTTGT ACCAAAACGT TGCAATGATA TGATGAACCT GGGTCGCCTC 
CAAGGCTTTG AGGGTAAGCT GACAGCCCAG GGCAAGCTGC TGCAGCAGGA CACCTTCTAC 
GTGACAGAGC ATGACTCCGG AGTTCAGTCC CGACCAAAGG AACGTAGGGT GTTTCTCTTT 
GAGCAAATAG TTATCTTCAG CGAGCTGCTT AGGAAAGGGT CTTTAACACC AGGCTACATG 
TTCAAGAGAA GCATAAAGAT GAACTACCTT GTCATTGAAG AAAATGTGGA TGGTGATCCC 
TGCAAGTTTG CTCTAATGAA TCGGGAAACA TCTGAGAAAG TTGTTTTACA GGCAGCTAAT 
CCAGAAATTC AGCAGGCTTG GGTACAGGAC ATCAACCAGG TCCTGGACAC ACAAAGGGAT 
TTCCTAAATG CACTGCAGTC TCCCATAGAG TACCAACGCA AAGAAAGTAG CACAGCAGTG 
CTGAGGCCCC AGGCTGGTAG GGTCGCTCAG CCCAACAGCA GACCCTATTC TTCTGTTCCA 
GTAGGGTCTG AGAAGCCTTT GAAGGCTACA AGCCGTAACC CATCTCTCCC ACCCCTGAAG 
ATATCTACCT CCAACGGCAG TACAGGGTAT GAACAGCCCG GAGACAAGTA TGAACAAAGC 
AAGACTGATT TGGGAGGGTG CAATGGGACA TCTTCCATGG TGGTGATTAA AGATTACTAT 
GCAGTGAAGG AGGACGAGAT CTGTGTGAAT CAAGGAGAGG TGGTTCAGAT CCTTGCTATC 
AACCAGCAAA ACATGTTCCT GGTGTACCAG CCTGCAAATG ACCACTCTCC AGCTGCTGAA 
GGATGGATCC CTGGCAATAT CTTGGCTCCC CTCACTAAAC CCACTACAGA TAATAGCGAC 
GGAAGCATCA AGAAGTCATG TTCATGGCAT ACCCTGCGCA TGAGAAAGCG GGCGGAAAAG 
GAGAGCAGTG GCAAAAATGA AGCCAATGGG CCACGTAAAT CCAAGGATAT TCTGGGGAAC 
AAAGTCTCTG TTAAAGAGAC AAACAGCTCA GAGGAATCAG AGTGTGATGA CCTTGACCCC 
AATACTAGCA TGGAGATAAT CAACCCCAAT TTCATTCAGG AAGTGGCTCC AGAGTTCCTT 
GTGCTGTTGG TGGATATCAC CTGCCTGCTT GGCGACACTG TGATGCTGCA GTGCAAAGTC 
TGCGGCCGGC CCAAACCAAC AATAACCTGG AAAGGACCTG ATCAAAACAT TCTTGACAAT 
GACAACAGCA CGGCCACATA CACGGTGTCA TCCTGTGACT CTGGAGAGCT CATGCTGAAG 
ATCTGCAATG TGATGCCTCA GGACAGTGGC ATTTACACTT GTGTGGCAAC TAACGAGCAT 
GGAACGGCAT CAACCTCAGC CACAATCAAA GTGCAAGGTG TACCGGCTGC CCCAAATCGC 
CCCATTGCTC AGGAGAGAAG CTGCACCTCT GTGATCCTTC GCTGGCTGCC TCCATCCAGT 
ACAGGCAATT GCACCATTTC TGGCTACACA GTGGAGTACA GAGAAGAAGG CTCACAGGTC 
TGGCAGCAGT CAGTGGCCTC CACACTGGAC ACTTACCTTG TCATTGAAGA CCTGTCCCCA 
GGCTGCCAGT ATCAGTTCCG TGTGAGTGCC AGCAACCCCT GGGGGATCAG CTTGCCCAGT 
GACCCATCTG AATTCGTGAG GCTTCCAGAG TATGATTCTG CTGCTGATGG TGCCACTATT 
TCGTGGAAGG AAAACTTTGA TCTCGCTTAT GCGGAGCTGC ATGAGATTGG GAGGGGACGA 
TTCTCCATTG TAAAGAAATG CGTTCACAAA GCTACCCGGA AAGACGTGGC TGTAAAATTC 
ATCAGTAAAA AAATGAAGAA GAAGGAGCAA GCAGCCCACG AGGCAGCTCT GTTACAGCAC 
TTACAGCATC CTCAGTACAT CACTATCCAT GATACCTATG AGTCTCCTAC TTCTTACATC 
CTAGTCCTGG AGCTGATGGA TGATGGTCGT CTCCTAGATT ATCTAATGAA CCATGATGAA 
CTTATGGAGG AAAAAGTAGC TTTCTACATA AGAGACACAA TGGAAGCCTT GCAGTATCTT 
CACAATTGCC GAGTGGCTCA TTTAGATATA AAGCCAGAGA ATCTGCTCAT CGATTTAAGG 
ATCCCAGTTC CTCGTGTCAA GATCATTGAT TTGGAAGATG CAGTTCAAAT CACAGGCCAT 
TACCACGTCC ACCATCTCCT TGGAAACCCA GAGTTTGCAG CCCCTGAAGT TATCCAGGGC 
CTCCCCGTGT CCCTGAGCAC AGACATCTGG AGCATCGGGG TCCTCACCTA CGTCATGCTG 
AGCGGTGTCT CCCCGTTCCT GGATGAAAGC AAAGAGGAGA CGTGTATCAA TGTGTGCAGA 
GTCGATTTCA GCTTCCCGCC CGAGTACTTC TCTGATGTGA GCCATGCTGC TAGGGATTTC 
ATCAACGTCA TCCTCCAGGA AGACTCCAGG AGGAGGCCGA CAGCAGCCAC TTGTTTACAG 
CACCCATGGC TGCAGCCACA CAATGGCAGC TACTCCAAAA TCCCGCTGGA TACCTCCCGC 
TTGGCATCCT TCATTGAGCG CCGCAGACAC CAGTACGACG TGCACCCCGT CCCCGGTGTC 
AAGAGTTTCC TGATGAGCAG GCTGAACCCC GGGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KALRN ( XM_015289977.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145463.1 Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X3, mRNA.

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CloneID	OGa38673
Clone ID Related Accession (Same CDS sequence)	XM_015289977.1 , XM_015289977.2
Accession Version	XM_015289977.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	kalirin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289977.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG AAGACACACC AGAGTGCCCT GCAGGTCCAG 
CAGAAAGCCG AAGTGCTGCT GCAGGCTGGG CATTATGATG CTGATGCCAT CAGAGAATGC 
GCTGAAAAGG TGGCCCTGCA CTGGCAGCAG CTGATGCTGA AGATGGAGGA CAGGCTCAAG 
CTGGTCAATG CCTCGGTGGC CTTCTACAAA ACCTCTGAGC AGGTGTGCAG TGTATTAGAG 
AGCCTGGAGC AAGAATACAG ACGTGATGAA GACTGGTGTG GAGGTCGAGA TAAACTTGGA 
CCAGCAGCTG AGATAGACCA CGTCATCCCT CTGATCAGCA AGCATCTGGA GCAGAAGGAG 
GCTTTTCTCA AGGCCTGCAC ACTGGCTCGA AGGAATGCTG AGGTATTCCT CAAGTACATC 
CACAGGAACA ATGTCAGCAT GCCCGGAGTC GCAAGCCATA CACGGGGACC TGAGCAGCAA 
GTGAAAGCCA TCCTGAGTGA GTTGCTGCAG AGGGAGAACC GGGTCCTTCA CTTCTGGACC 
CTGAAGAAGC GGAGGTTAGA TCAGTGCCAG CAGTACGTTG TATTTGAGCG CAGTGCCAAG 
CAGGCCCTGG ACTGGATCCA AGAGACAGGC GAATATTACC TCTCTACTCA CAACTCTACA 
GGGGAATCAG CAGAAGAAAC TCAGGAACTC CTGAAGGAAT ATGGGGAATT CAGAGTACCT 
GCGAAGCAAA CAAAGGAGAA AGTGAAGCTC CTCATCCAGC TGGCTGACAG CTTTGTAGAA 
AAAGGACATA TACACGCCAC TGAAATCAGG AAGTGGGTCA CCACCGTTGA CAAACGCTAC 
CGTGACTTCT CTCTCAGGAT GGGGAAATAC CGCTACTCCC TGGAGAAAGC CCTTGGAATC 
AATACAGAGG ATAACAAGGA CCTCGAACTA GATATTATTC CAGCAAGTCT TTCAGACCGG 
GAGGTAAAGC TGCGAGATGC AAATCACGAA GTCAATGAAG AAAAAAGAAA GTCTGCTAGA 
AAGAAAGAAT TCATTATGGC CGAGCTTCTT CAGACAGAGA AAGCTTATGT CAGGGACTTA 
CATGAATGTT TAGAGACTTA CCTTTGGGAA ATGACAAGTG GGGTGGAAGA AATACCCGCT 
GGGATCCTTA ATAAAGAACA CATCATCTTT GGCAATATTC AGGAGATATA TGACTTCCAT 
AACAACATTT TCCTGAAAGA ACTCGAGAAG TATGAGCAAC TGCCTGAGGA TGTGGGTCAC 
TGCTTTGTCA CCTGGGCAGA TAAATTTCAG ATGTATGTCA CCTACTGCAA AAACAAGCCT 
GACTCCAGCC AGCTCATCCT GGAACACGCA GGGACTTTCT TTGATGAAAT TCAGCAAAGG 
CATGGTCTGG CCAACTCTAT CTCTTCTTAT TTAATCAAGC CTGTCCAGAG GATCACAAAA 
TATCAACTCC TCCTGAAGGA ACTGCTTACC TGCTGTGAGG AAGGCAAAGG GGAACTCAAG 
GATGGTCTGG AAGTGATGCT CAGTGTCCCC AAGAAAGCCA ATGATGCAAT GCACGTCAGC 
ATGCTGGAAG GGTTTGATGA GAATCTAGAC GTCCAGGGAG AGCTGATTCT TCAGGATTCC 
TTCCAAGTGT GGGACCCCAA GTCTCTCATT CGGAAAGGCC GTGAGAGACA TTTGTTTCTC 
TTTGAAATCT CTTTGGTGTT TAGCAAAGAG ATCAAAGACT CTTCAGGACA CACAAAATAT 
GTTTACAAGA ACAAGTTACT GACCTCAGAA CTGGGAGTGA CTGAACATGT TGAAGGAGAT 
CCCTGTAAAT TTGCCTTGTG GTCTGGACGA ACACCATCCT CAGACAATAA AACAGTGCTG 
AAAGCATCGA GTATTGAAAC AAAACAGGAG TGGATCAAAA ATATCCGAGA AGTCATCCAA 
GAAAGAATTA TCCATCTGAA AGGAGCTCTG AAAGAGCCAA TTCAGCTCCC CAAGACACCA 
GCAAAGCAGC GAAACAACAG TAGAAGAGAT GGAGTAGATG ATGCAGACAG CCAAGGAGAT 
GGAAGCAGTC AACCTGACAC CATTTCCATT GCATCCAGGA CATCACAGAA TACAGTGGAC 
AGTGATAAGC ATTTCGTCTA CGGCAGAGAC ACAGGACAGT TGTCTGGAGG GTGTGAACTA 
ACCGTGGTGC TCCAGGACTT CACGGCGGGC CACAGCAGCG AGCTGAGCAT TCAGGTGGGG 
CAGACAGTGG AGCTGCTGGA GAGGCCAAGT GAAAGGCCAG GCTGGTGCTT GGTGCGGACC 
ACGGAGCGGA GCCCACCTCA GGAAGGCCTG GTCCCCAGCA GCGCTCTCTG CATTTCCCAT 
TCCCGCAGCA GCGTGGAGAT GGATTGCTTC TTCCCTTCAG GAAAAGAGGC ATATTCAGTC 
TCTTCCAATG AAAACGGGGG CAAGTCCGAT TCAGTGGCCA ACTTACAGCC CCAGCCATCC 
CTCAACTCCA TCCAGAGCTC TCCCGGCCCC AAGCGCTCGA CCAACACGCT GAAGAAATGG 
CTGACGAGCC CCGTGCGCCG GCTGAACAGC GGGAAGGCCG AGAGCAACAT CAAGAAGCAA 
AAGAAGGTGC GAGATGGCAG GAAGAGCTTT GACCTCGGCT CGCCGAAGAC TGGAGATGAA 
ACCACTCCTC AAGGAGACAG CGCGGATGAG AAGAGCAAGA AGGGTTGGGG AGAAGATGAG 
CCAGATGAAG AGTCTCACAC ACCTCTTCCT CCTCCTATGA AGATTTTTGA CAATGATCCA 
ACCCAAGATG AGATGTCCTC TTCTTTGCTA GCTGCTCGGC AGAGCTCCTC CGATGTCCCA 
ACTGCTGCGG ATCTTGTCAG TGCAATAGAA CAGTTGGTCA AAAGTAAGCT GACCCTTGAA 
GGAGGATCTT ACCGAGGAAG CTTAAAAGAT GCCACAGGCT GCTTGAATGA AGGAATGGCG 
CCTTCAACTC CCCCAAGAAA CCTTGAAGAG GAACAAAAAG CCAAAGCAAT GAGAGGCAGG 
ATGTTTGTCT TAAATGAACT GGTCCAGACT GAAAAAGACT ACGTCAAAGA CTTGGGAACC 
GTTGTGGAGG GCTTCATGAA GAGAATAGAA GAAAAGGGAG TTTCCGAAGA TATGAAAGGG 
AAAGACAAGA TAGTGTTTGG CAATATTCAC CAGATCTATG ACTGGCACAA AGACTTTTTC 
CTGGCTGAAC TGGAAAAATG TCTTCAGGAG CCTGACCGTT TAGCACAGCT TTTTATTAAA 
CATGAGCGGC GATTACACAT GTATGTGGTG TACTGCCAAA ACAAGCCACG ATCTGAATAT 
ATAGTAGCTG AATACGAGGC ATATTTTGAG GAGGTTCAAC AGGAAATAAA CCAACGGCTG 
ACCATCAGTG ACTTTCTGAT CAAACCCATT CAGAGAATAA CTAAATATCA GCTTCTTCTC 
AAGGACTTCC TGAGGTACAG TGAAAAAGCT GGTCTGGAGT GCTCTGAAAT AGAGAAAGCA 
GTTGAGTTAA TGTGCCTTGT ACCAAAACGT TGCAATGATA TGATGAACCT GGGTCGCCTC 
CAAGGCTTTG AGGGTAAGCT GACAGCCCAG GGCAAGCTGC TGCAGCAGGA CACCTTCTAC 
GTGACAGAGC ATGACTCCGG AGTTCAGTCC CGACCAAAGG AACGTAGGGT GTTTCTCTTT 
GAGCAAATAG TTATCTTCAG CGAGCTGCTT AGGAAAGGGT CTTTAACACC AGGCTACATG 
TTCAAGAGAA GCATAAAGAT GAACTACCTT GTCATTGAAG AAAATGTGGA TGGTGATCCC 
TGCAAGTTTG CTCTAATGAA TCGGGAAACA TCTGAGAAAG TTGTTTTACA GGCAGCTAAT 
CCAGAAATTC AGCAGGCTTG GGTACAGGAC ATCAACCAGG TCCTGGACAC ACAAAGGGAT 
TTCCTAAATG CACTGCAGTC TCCCATAGAG TACCAACGCA AAGAAAGTAG CACAGCAGTG 
CTGAGGCCCC AGGCTGGTAG GGTCGCTCAG CCCAACAGCA GACCCTATTC TTCTGTTCCA 
GTAGGGTCTG AGAAGCCTTT GAAGGCTACA AGCCGTAACC CATCTCTCCC ACCCCTGAAG 
ATATCTACCT CCAACGGCAG TACAGGGTAT GAACAGCCCG GAGACAAGTA TGAACAAAGC 
AAGACTGATT TGGGAGGGTG CAATGGGACA TCTTCCATGG TGGTGATTAA AGATTACTAT 
GCAGTGAAGG AGGACGAGAT CTGTGTGAAT CAAGGAGAGG TGGTTCAGAT CCTTGCTATC 
AACCAGCAAA ACATGTTCCT GGTGTACCAG CCTGCAAATG ACCACTCTCC AGCTGCTGAA 
GGATGGATCC CTGGCAATAT CTTGGCTCCC CTCACTAAAC CCACTACAGA TAATAGCGAC 
GGAAGCATCA AGAAGTCATG TTCATGGCAT ACCCTGCGCA TGAGAAAGCG GGCGGAAAAG 
GAGAGCAGTG GCAAAAATGA AGCCAATGGG CCACGTAAAT CCAAGGATAT TCTGGGGAAC 
AAAGTCTCTG TTAAAGAGAC AAACAGCTCA GAGGAATCAG AGTGTGATGA CCTTGACCCC 
AATACTAGCA TGGAGATAAT CAACCCCAAT TTCATTCAGG AAGTGGCTCC AGAGTTCCTT 
GTGCTGTTGG TGGATATCAC CTGCCTGCTT GGCGACACTG TGATGCTGCA GTGCAAAGTC 
TGCGGCCGGC CCAAACCAAC AATAACCTGG AAAGGACCTG ATCAAAACAT TCTTGACAAT 
GACAACAGCA CGGCCACATA CACGGTGTCA TCCTGTGACT CTGGAGAGCT CATGCTGAAG 
ATCTGCAATG TGATGCCTCA GGACAGTGGC ATTTACACTT GTGTGGCAAC TAACGAGCAT 
GGAACGGCAT CAACCTCAGC CACAATCAAA GTGCAAGGTG TACCGGCTGC CCCAAATCGC 
CCCATTGCTC AGGAGAGAAG CTGCACCTCT GTGATCCTTC GCTGGCTGCC TCCATCCAGT 
ACAGGCAATT GCACCATTTC TGGCTACACA GTGGAGTACA GAGAAGAAGG CTCACAGGTC 
TGGCAGCAGT CAGTGGCCTC CACACTGGAC ACTTACCTTG TCATTGAAGA CCTGTCCCCA 
GGCTGCCAGT ATCAGTTCCG TGTGAGTGCC AGCAACCCCT GGGGGATCAG CTTGCCCAGT 
GACCCATCTG AATTCGTGAG GCTTCCAGAG TATGATTCTG CTGCTGATGG TGCCACTATT 
TCGTGGAAGG AAAACTTTGA TCTCGCTTAT GCGGAGCTGC ATGAGATTGG GAGGGGACGA 
TTCTCCATTG TAAAGAAATG CGTTCACAAA GCTACCCGGA AAGACGTGGC TGTAAAATTC 
ATCAGTAAAA AAATGAAGAA GAAGGAGCAA GCAGCCCACG AGGCAGCTCT GTTACAGCAC 
TTACAGCATC CTCAGTACAT CACTATCCAT GATACCTATG AGTCTCCTAC TTCTTACATC 
CTAGTCCTGG AGCTGATGGA TGATGGTCGT CTCCTAGATT ATCTAATGAA CCATGATGAA 
CTTATGGAGG AAAAAGTAGC TTTCTACATA AGAGACACAA TGGAAGCCTT GCAGTATCTT 
CACAATTGCC GAGTGGCTCA TTTAGATATA AAGCCAGAGA ATCTGCTCAT CGATTTAAGG 
ATCCCAGTTC CTCGTGTCAA GATCATTGAT TTGGAAGATG CAGTTCAAAT CACAGGCCAT 
TACCACGTCC ACCATCTCCT TGGAAACCCA GAGTTTGCAG CCCCTGAAGT TATCCAGGGC 
CTCCCCGTGT CCCTGAGCAC AGACATCTGG AGCATCGGGG TCCTCACCTA CGTCATGCTG 
AGCGGTGTCT CCCCGTTCCT GGATGAAAGC AAAGAGGAGA CGTGTATCAA TGTGTGCAGA 
GTCGATTTCA GCTTCCCGCC CGAGTACTTC TCTGATGTGA GCCATGCTGC TAGGGATTTC 
ATCAACGTCA TCCTCCAGGA AGACTCCAGG AGGAGGCCGA CAGCAGCCAC TTGTTTACAG 
CACCCATGGC TGCAGCCACA CAATGGCAGC TACTCCAAAA TCCCGCTGGA TACCTCCCGC 
TTGGCATCCT TCATTGAGCG CCGCAGACAC CAGTACGACG TGCACCCCGT CCCCGGTGTC 
AAGAGTTTCC TGATGAGCAG GCTGAACCCC GGGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145463.1
CDS	244..9162
Translation	MNPTEGAAEEGAVPDSEVDAFFRTGSFRNDGLKAADVLPILKEKVAFVSGGRDKRGGPVLTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLAKLVDPSQLTDEFEGSLDYNHDEWIELRVSLEEFFNSAIHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVIKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKELLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAVSNNKTPHNSSISHIESVLQQLDEAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDIDLATQVQELLEFLHEKQHELELNADQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIEKTHQSALQVQQKAEVLLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEIDHVIPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPGVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHNSTGESAEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKRYRDFSLRMGKYRYSLEKALGINTEDNKDLELDIIPASLSDREVKLRDANHEVNEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPAGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSSQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDSFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASSIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKQRNNSRRDGVDDADSQGDGSSQPDTISIASRTSQNTVDSDKHFVYGRDTGQLSGGCELTVVLQDFTAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPSGKEAYSVSSNENGGKSDSVANLQPQPSLNSIQSSPGPKRSTNTLKKWLTSPVRRLNSGKAESNIKKQKKVRDGRKSFDLGSPKTGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSSLLAARQSSSDVPTAADLVSAIEQLVKSKLTLEGGSYRGSLKDATGCLNEGMAPSTPPRNLEEEQKAKAMRGRMFVLNELVQTEKDYVKDLGTVVEGFMKRIEEKGVSEDMKGKDKIVFGNIHQIYDWHKDFFLAELEKCLQEPDRLAQLFIKHERRLHMYVVYCQNKPRSEYIVAEYEAYFEEVQQEINQRLTISDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSEIEKAVELMCLVPKRCNDMMNLGRLQGFEGKLTAQGKLLQQDTFYVTEHDSGVQSRPKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVIEENVDGDPCKFALMNRETSEKVVLQAANPEIQQAWVQDINQVLDTQRDFLNALQSPIEYQRKESSTAVLRPQAGRVAQPNSRPYSSVPVGSEKPLKATSRNPSLPPLKISTSNGSTGYEQPGDKYEQSKTDLGGCNGTSSMVVIKDYYAVKEDEICVNQGEVVQILAINQQNMFLVYQPANDHSPAAEGWIPGNILAPLTKPTTDNSDGSIKKSCSWHTLRMRKRAEKESSGKNEANGPRKSKDILGNKVSVKETNSSEESECDDLDPNTSMEIINPNFIQEVAPEFLVLLVDITCLLGDTVMLQCKVCGRPKPTITWKGPDQNILDNDNSTATYTVSSCDSGELMLKICNVMPQDSGIYTCVATNEHGTASTSATIKVQGVPAAPNRPIAQERSCTSVILRWLPPSSTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCQYQFRVSASNPWGISLPSDPSEFVRLPEYDSAADGATISWKENFDLAYAELHEIGRGRFSIVKKCVHKATRKDVAVKFISKKMKKKEQAAHEAALLQHLQHPQYITIHDTYESPTSYILVLELMDDGRLLDYLMNHDELMEEKVAFYIRDTMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKIIDLEDAVQITGHYHVHHLLGNPEFAAPEVIQGLPVSLSTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPPEYFSDVSHAARDFINVILQEDSRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLASFIERRRHQYDVHPVPGVKSFLMSRLNPGT

Target ORF information:

RefSeq Version	XM_015289977.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289977.2

ORF Insert Sequence:

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG AAGACACACC AGAGTGCCCT GCAGGTCCAG 
CAGAAAGCCG AAGTGCTGCT GCAGGCTGGG CATTATGATG CTGATGCCAT CAGAGAATGC 
GCTGAAAAGG TGGCCCTGCA CTGGCAGCAG CTGATGCTGA AGATGGAGGA CAGGCTCAAG 
CTGGTCAATG CCTCGGTGGC CTTCTACAAA ACCTCTGAGC AGGTGTGCAG TGTATTAGAG 
AGCCTGGAGC AAGAATACAG ACGTGATGAA GACTGGTGTG GAGGTCGAGA TAAACTTGGA 
CCAGCAGCTG AGATAGACCA CGTCATCCCT CTGATCAGCA AGCATCTGGA GCAGAAGGAG 
GCTTTTCTCA AGGCCTGCAC ACTGGCTCGA AGGAATGCTG AGGTATTCCT CAAGTACATC 
CACAGGAACA ATGTCAGCAT GCCCGGAGTC GCAAGCCATA CACGGGGACC TGAGCAGCAA 
GTGAAAGCCA TCCTGAGTGA GTTGCTGCAG AGGGAGAACC GGGTCCTTCA CTTCTGGACC 
CTGAAGAAGC GGAGGTTAGA TCAGTGCCAG CAGTACGTTG TATTTGAGCG CAGTGCCAAG 
CAGGCCCTGG ACTGGATCCA AGAGACAGGC GAATATTACC TCTCTACTCA CAACTCTACA 
GGGGAATCAG CAGAAGAAAC TCAGGAACTC CTGAAGGAAT ATGGGGAATT CAGAGTACCT 
GCGAAGCAAA CAAAGGAGAA AGTGAAGCTC CTCATCCAGC TGGCTGACAG CTTTGTAGAA 
AAAGGACATA TACACGCCAC TGAAATCAGG AAGTGGGTCA CCACCGTTGA CAAACGCTAC 
CGTGACTTCT CTCTCAGGAT GGGGAAATAC CGCTACTCCC TGGAGAAAGC CCTTGGAATC 
AATACAGAGG ATAACAAGGA CCTCGAACTA GATATTATTC CAGCAAGTCT TTCAGACCGG 
GAGGTAAAGC TGCGAGATGC AAATCACGAA GTCAATGAAG AAAAAAGAAA GTCTGCTAGA 
AAGAAAGAAT TCATTATGGC CGAGCTTCTT CAGACAGAGA AAGCTTATGT CAGGGACTTA 
CATGAATGTT TAGAGACTTA CCTTTGGGAA ATGACAAGTG GGGTGGAAGA AATACCCGCT 
GGGATCCTTA ATAAAGAACA CATCATCTTT GGCAATATTC AGGAGATATA TGACTTCCAT 
AACAACATTT TCCTGAAAGA ACTCGAGAAG TATGAGCAAC TGCCTGAGGA TGTGGGTCAC 
TGCTTTGTCA CCTGGGCAGA TAAATTTCAG ATGTATGTCA CCTACTGCAA AAACAAGCCT 
GACTCCAGCC AGCTCATCCT GGAACACGCA GGGACTTTCT TTGATGAAAT TCAGCAAAGG 
CATGGTCTGG CCAACTCTAT CTCTTCTTAT TTAATCAAGC CTGTCCAGAG GATCACAAAA 
TATCAACTCC TCCTGAAGGA ACTGCTTACC TGCTGTGAGG AAGGCAAAGG GGAACTCAAG 
GATGGTCTGG AAGTGATGCT CAGTGTCCCC AAGAAAGCCA ATGATGCAAT GCACGTCAGC 
ATGCTGGAAG GGTTTGATGA GAATCTAGAC GTCCAGGGAG AGCTGATTCT TCAGGATTCC 
TTCCAAGTGT GGGACCCCAA GTCTCTCATT CGGAAAGGCC GTGAGAGACA TTTGTTTCTC 
TTTGAAATCT CTTTGGTGTT TAGCAAAGAG ATCAAAGACT CTTCAGGACA CACAAAATAT 
GTTTACAAGA ACAAGTTACT GACCTCAGAA CTGGGAGTGA CTGAACATGT TGAAGGAGAT 
CCCTGTAAAT TTGCCTTGTG GTCTGGACGA ACACCATCCT CAGACAATAA AACAGTGCTG 
AAAGCATCGA GTATTGAAAC AAAACAGGAG TGGATCAAAA ATATCCGAGA AGTCATCCAA 
GAAAGAATTA TCCATCTGAA AGGAGCTCTG AAAGAGCCAA TTCAGCTCCC CAAGACACCA 
GCAAAGCAGC GAAACAACAG TAGAAGAGAT GGAGTAGATG ATGCAGACAG CCAAGGAGAT 
GGAAGCAGTC AACCTGACAC CATTTCCATT GCATCCAGGA CATCACAGAA TACAGTGGAC 
AGTGATAAGC ATTTCGTCTA CGGCAGAGAC ACAGGACAGT TGTCTGGAGG GTGTGAACTA 
ACCGTGGTGC TCCAGGACTT CACGGCGGGC CACAGCAGCG AGCTGAGCAT TCAGGTGGGG 
CAGACAGTGG AGCTGCTGGA GAGGCCAAGT GAAAGGCCAG GCTGGTGCTT GGTGCGGACC 
ACGGAGCGGA GCCCACCTCA GGAAGGCCTG GTCCCCAGCA GCGCTCTCTG CATTTCCCAT 
TCCCGCAGCA GCGTGGAGAT GGATTGCTTC TTCCCTTCAG GAAAAGAGGC ATATTCAGTC 
TCTTCCAATG AAAACGGGGG CAAGTCCGAT TCAGTGGCCA ACTTACAGCC CCAGCCATCC 
CTCAACTCCA TCCAGAGCTC TCCCGGCCCC AAGCGCTCGA CCAACACGCT GAAGAAATGG 
CTGACGAGCC CCGTGCGCCG GCTGAACAGC GGGAAGGCCG AGAGCAACAT CAAGAAGCAA 
AAGAAGGTGC GAGATGGCAG GAAGAGCTTT GACCTCGGCT CGCCGAAGAC TGGAGATGAA 
ACCACTCCTC AAGGAGACAG CGCGGATGAG AAGAGCAAGA AGGGTTGGGG AGAAGATGAG 
CCAGATGAAG AGTCTCACAC ACCTCTTCCT CCTCCTATGA AGATTTTTGA CAATGATCCA 
ACCCAAGATG AGATGTCCTC TTCTTTGCTA GCTGCTCGGC AGAGCTCCTC CGATGTCCCA 
ACTGCTGCGG ATCTTGTCAG TGCAATAGAA CAGTTGGTCA AAAGTAAGCT GACCCTTGAA 
GGAGGATCTT ACCGAGGAAG CTTAAAAGAT GCCACAGGCT GCTTGAATGA AGGAATGGCG 
CCTTCAACTC CCCCAAGAAA CCTTGAAGAG GAACAAAAAG CCAAAGCAAT GAGAGGCAGG 
ATGTTTGTCT TAAATGAACT GGTCCAGACT GAAAAAGACT ACGTCAAAGA CTTGGGAACC 
GTTGTGGAGG GCTTCATGAA GAGAATAGAA GAAAAGGGAG TTTCCGAAGA TATGAAAGGG 
AAAGACAAGA TAGTGTTTGG CAATATTCAC CAGATCTATG ACTGGCACAA AGACTTTTTC 
CTGGCTGAAC TGGAAAAATG TCTTCAGGAG CCTGACCGTT TAGCACAGCT TTTTATTAAA 
CATGAGCGGC GATTACACAT GTATGTGGTG TACTGCCAAA ACAAGCCACG ATCTGAATAT 
ATAGTAGCTG AATACGAGGC ATATTTTGAG GAGGTTCAAC AGGAAATAAA CCAACGGCTG 
ACCATCAGTG ACTTTCTGAT CAAACCCATT CAGAGAATAA CTAAATATCA GCTTCTTCTC 
AAGGACTTCC TGAGGTACAG TGAAAAAGCT GGTCTGGAGT GCTCTGAAAT AGAGAAAGCA 
GTTGAGTTAA TGTGCCTTGT ACCAAAACGT TGCAATGATA TGATGAACCT GGGTCGCCTC 
CAAGGCTTTG AGGGTAAGCT GACAGCCCAG GGCAAGCTGC TGCAGCAGGA CACCTTCTAC 
GTGACAGAGC ATGACTCCGG AGTTCAGTCC CGACCAAAGG AACGTAGGGT GTTTCTCTTT 
GAGCAAATAG TTATCTTCAG CGAGCTGCTT AGGAAAGGGT CTTTAACACC AGGCTACATG 
TTCAAGAGAA GCATAAAGAT GAACTACCTT GTCATTGAAG AAAATGTGGA TGGTGATCCC 
TGCAAGTTTG CTCTAATGAA TCGGGAAACA TCTGAGAAAG TTGTTTTACA GGCAGCTAAT 
CCAGAAATTC AGCAGGCTTG GGTACAGGAC ATCAACCAGG TCCTGGACAC ACAAAGGGAT 
TTCCTAAATG CACTGCAGTC TCCCATAGAG TACCAACGCA AAGAAAGTAG CACAGCAGTG 
CTGAGGCCCC AGGCTGGTAG GGTCGCTCAG CCCAACAGCA GACCCTATTC TTCTGTTCCA 
GTAGGGTCTG AGAAGCCTTT GAAGGCTACA AGCCGTAACC CATCTCTCCC ACCCCTGAAG 
ATATCTACCT CCAACGGCAG TACAGGGTAT GAACAGCCCG GAGACAAGTA TGAACAAAGC 
AAGACTGATT TGGGAGGGTG CAATGGGACA TCTTCCATGG TGGTGATTAA AGATTACTAT 
GCAGTGAAGG AGGACGAGAT CTGTGTGAAT CAAGGAGAGG TGGTTCAGAT CCTTGCTATC 
AACCAGCAAA ACATGTTCCT GGTGTACCAG CCTGCAAATG ACCACTCTCC AGCTGCTGAA 
GGATGGATCC CTGGCAATAT CTTGGCTCCC CTCACTAAAC CCACTACAGA TAATAGCGAC 
GGAAGCATCA AGAAGTCATG TTCATGGCAT ACCCTGCGCA TGAGAAAGCG GGCGGAAAAG 
GAGAGCAGTG GCAAAAATGA AGCCAATGGG CCACGTAAAT CCAAGGATAT TCTGGGGAAC 
AAAGTCTCTG TTAAAGAGAC AAACAGCTCA GAGGAATCAG AGTGTGATGA CCTTGACCCC 
AATACTAGCA TGGAGATAAT CAACCCCAAT TTCATTCAGG AAGTGGCTCC AGAGTTCCTT 
GTGCTGTTGG TGGATATCAC CTGCCTGCTT GGCGACACTG TGATGCTGCA GTGCAAAGTC 
TGCGGCCGGC CCAAACCAAC AATAACCTGG AAAGGACCTG ATCAAAACAT TCTTGACAAT 
GACAACAGCA CGGCCACATA CACGGTGTCA TCCTGTGACT CTGGAGAGCT CATGCTGAAG 
ATCTGCAATG TGATGCCTCA GGACAGTGGC ATTTACACTT GTGTGGCAAC TAACGAGCAT 
GGAACGGCAT CAACCTCAGC CACAATCAAA GTGCAAGGTG TACCGGCTGC CCCAAATCGC 
CCCATTGCTC AGGAGAGAAG CTGCACCTCT GTGATCCTTC GCTGGCTGCC TCCATCCAGT 
ACAGGCAATT GCACCATTTC TGGCTACACA GTGGAGTACA GAGAAGAAGG CTCACAGGTC 
TGGCAGCAGT CAGTGGCCTC CACACTGGAC ACTTACCTTG TCATTGAAGA CCTGTCCCCA 
GGCTGCCAGT ATCAGTTCCG TGTGAGTGCC AGCAACCCCT GGGGGATCAG CTTGCCCAGT 
GACCCATCTG AATTCGTGAG GCTTCCAGAG TATGATTCTG CTGCTGATGG TGCCACTATT 
TCGTGGAAGG AAAACTTTGA TCTCGCTTAT GCGGAGCTGC ATGAGATTGG GAGGGGACGA 
TTCTCCATTG TAAAGAAATG CGTTCACAAA GCTACCCGGA AAGACGTGGC TGTAAAATTC 
ATCAGTAAAA AAATGAAGAA GAAGGAGCAA GCAGCCCACG AGGCAGCTCT GTTACAGCAC 
TTACAGCATC CTCAGTACAT CACTATCCAT GATACCTATG AGTCTCCTAC TTCTTACATC 
CTAGTCCTGG AGCTGATGGA TGATGGTCGT CTCCTAGATT ATCTAATGAA CCATGATGAA 
CTTATGGAGG AAAAAGTAGC TTTCTACATA AGAGACACAA TGGAAGCCTT GCAGTATCTT 
CACAATTGCC GAGTGGCTCA TTTAGATATA AAGCCAGAGA ATCTGCTCAT CGATTTAAGG 
ATCCCAGTTC CTCGTGTCAA GATCATTGAT TTGGAAGATG CAGTTCAAAT CACAGGCCAT 
TACCACGTCC ACCATCTCCT TGGAAACCCA GAGTTTGCAG CCCCTGAAGT TATCCAGGGC 
CTCCCCGTGT CCCTGAGCAC AGACATCTGG AGCATCGGGG TCCTCACCTA CGTCATGCTG 
AGCGGTGTCT CCCCGTTCCT GGATGAAAGC AAAGAGGAGA CGTGTATCAA TGTGTGCAGA 
GTCGATTTCA GCTTCCCGCC CGAGTACTTC TCTGATGTGA GCCATGCTGC TAGGGATTTC 
ATCAACGTCA TCCTCCAGGA AGACTCCAGG AGGAGGCCGA CAGCAGCCAC TTGTTTACAG 
CACCCATGGC TGCAGCCACA CAATGGCAGC TACTCCAAAA TCCCGCTGGA TACCTCCCGC 
TTGGCATCCT TCATTGAGCG CCGCAGACAC CAGTACGACG TGCACCCCGT CCCCGGTGTC 
AAGAGTTTCC TGATGAGCAG GCTGAACCCC GGGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KALRN ( XM_015289978.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145464.1 Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X4, mRNA.

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CloneID	OGa38674
Clone ID Related Accession (Same CDS sequence)	XM_015289978.2 , XM_015289978.1
Accession Version	XM_015289978.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	kalirin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289978.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGTTGT CTGGAGGGTG TGAACTAACC 
GTGGTGCTCC AGGACTTCAC GGCGGGCCAC AGCAGCGAGC TGAGCATTCA GGTGGGGCAG 
ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA AGGCCAGGCT GGTGCTTGGT GCGGACCACG 
GAGCGGAGCC CACCTCAGGA AGGCCTGGTC CCCAGCAGCG CTCTCTGCAT TTCCCATTCC 
CGCAGCAGCG TGGAGATGGA TTGCTTCTTC CCTTCAGGAA AAGAGGCATA TTCAGTCTCT 
TCCAATGAAA ACGGGGGCAA GTCCGATTCA GTGGCCAACT TACAGCCCCA GCCATCCCTC 
AACTCCATCC AGAGCTCTCC CGGCCCCAAG CGCTCGACCA ACACGCTGAA GAAATGGCTG 
ACGAGCCCCG TGCGCCGGCT GAACAGCGGG AAGGCCGAGA GCAACATCAA GAAGCAAAAG 
AAGGTGCGAG ATGGCAGGAA GAGCTTTGAC CTCGGCTCGC CGAAGACTGG AGATGAAACC 
ACTCCTCAAG GAGACAGCGC GGATGAGAAG AGCAAGAAGG GTTGGGGAGA AGATGAGCCA 
GATGAAGAGT CTCACACACC TCTTCCTCCT CCTATGAAGA TTTTTGACAA TGATCCAACC 
CAAGATGAGA TGTCCTCTTC TTTGCTAGCT GCTCGGCAGA GCTCCTCCGA TGTCCCAACT 
GCTGCGGATC TTGTCAGTGC AATAGAACAG TTGGTCAAAA GTAAGCTGAC CCTTGAAGGA 
GGATCTTACC GAGGAAGCTT AAAAGATGCC ACAGGCTGCT TGAATGAAGG AATGGCGCCT 
TCAACTCCCC CAAGAAACCT TGAAGAGGAA CAAAAAGCCA AAGCAATGAG AGGCAGGATG 
TTTGTCTTAA ATGAACTGGT CCAGACTGAA AAAGACTACG TCAAAGACTT GGGAACCGTT 
GTGGAGGGCT TCATGAAGAG AATAGAAGAA AAGGGAGTTT CCGAAGATAT GAAAGGGAAA 
GACAAGATAG TGTTTGGCAA TATTCACCAG ATCTATGACT GGCACAAAGA CTTTTTCCTG 
GCTGAACTGG AAAAATGTCT TCAGGAGCCT GACCGTTTAG CACAGCTTTT TATTAAACAT 
GAGCGGCGAT TACACATGTA TGTGGTGTAC TGCCAAAACA AGCCACGATC TGAATATATA 
GTAGCTGAAT ACGAGGCATA TTTTGAGGAG GTTCAACAGG AAATAAACCA ACGGCTGACC 
ATCAGTGACT TTCTGATCAA ACCCATTCAG AGAATAACTA AATATCAGCT TCTTCTCAAG 
GACTTCCTGA GGTACAGTGA AAAAGCTGGT CTGGAGTGCT CTGAAATAGA GAAAGCAGTT 
GAGTTAATGT GCCTTGTACC AAAACGTTGC AATGATATGA TGAACCTGGG TCGCCTCCAA 
GGCTTTGAGG GTAAGCTGAC AGCCCAGGGC AAGCTGCTGC AGCAGGACAC CTTCTACGTG 
ACAGAGCATG ACTCCGGAGT TCAGTCCCGA CCAAAGGAAC GTAGGGTGTT TCTCTTTGAG 
CAAATAGTTA TCTTCAGCGA GCTGCTTAGG AAAGGGTCTT TAACACCAGG CTACATGTTC 
AAGAGAAGCA TAAAGATGAA CTACCTTGTC ATTGAAGAAA ATGTGGATGG TGATCCCTGC 
AAGTTTGCTC TAATGAATCG GGAAACATCT GAGAAAGTTG TTTTACAGGC AGCTAATCCA 
GAAATTCAGC AGGCTTGGGT ACAGGACATC AACCAGGTCC TGGACACACA AAGGGATTTC 
CTAAATGCAC TGCAGTCTCC CATAGAGTAC CAACGCAAAG AAAGTAGCAC AGCAGTGCTG 
AGGCCCCAGG CTGGTAGGGT CGCTCAGCCC AACAGCAGAC CCTATTCTTC TGTTCCAGTA 
GGGTCTGAGA AGCCTTTGAA GGCTACAAGC CGTAACCCAT CTCTCCCACC CCTGAAGATA 
TCTACCTCCA ACGGCAGTAC AGGGTATGAA CAGCCCGGAG ACAAGTATGA ACAAAGCAAG 
ACTGATTTGG GAGGGTGCAA TGGGACATCT TCCATGGTGG TGATTAAAGA TTACTATGCA 
GTGAAGGAGG ACGAGATCTG TGTGAATCAA GGAGAGGTGG TTCAGATCCT TGCTATCAAC 
CAGCAAAACA TGTTCCTGGT GTACCAGCCT GCAAATGACC ACTCTCCAGC TGCTGAAGGA 
TGGATCCCTG GCAATATCTT GGCTCCCCTC ACTAAACCCA CTACAGATAA TAGCGACGGA 
AGCATCAAGA AGTCATGTTC ATGGCATACC CTGCGCATGA GAAAGCGGGC GGAAAAGGAG 
AGCAGTGGCA AAAATGAAGC CAATGGGCCA CGTAAATCCA AGGATATTCT GGGGAACAAA 
GTCTCTGTTA AAGAGACAAA CAGCTCAGAG GAATCAGAGT GTGATGACCT TGACCCCAAT 
ACTAGCATGG AGATAATCAA CCCCAATTTC ATTCAGGAAG TGGCTCCAGA GTTCCTTGTG 
CTGTTGGTGG ATATCACCTG CCTGCTTGGC GACACTGTGA TGCTGCAGTG CAAAGTCTGC 
GGCCGGCCCA AACCAACAAT AACCTGGAAA GGACCTGATC AAAACATTCT TGACAATGAC 
AACAGCACGG CCACATACAC GGTGTCATCC TGTGACTCTG GAGAGCTCAT GCTGAAGATC 
TGCAATGTGA TGCCTCAGGA CAGTGGCATT TACACTTGTG TGGCAACTAA CGAGCATGGA 
ACGGCATCAA CCTCAGCCAC AATCAAAGTG CAAGGTGTAC CGGCTGCCCC AAATCGCCCC 
ATTGCTCAGG AGAGAAGCTG CACCTCTGTG ATCCTTCGCT GGCTGCCTCC ATCCAGTACA 
GGCAATTGCA CCATTTCTGG CTACACAGTG GAGTACAGAG AAGAAGGCTC ACAGGTCTGG 
CAGCAGTCAG TGGCCTCCAC ACTGGACACT TACCTTGTCA TTGAAGACCT GTCCCCAGGC 
TGCCAGTATC AGTTCCGTGT GAGTGCCAGC AACCCCTGGG GGATCAGCTT GCCCAGTGAC 
CCATCTGAAT TCGTGAGGCT TCCAGAGTAT GATTCTGCTG CTGATGGTGC CACTATTTCG 
TGGAAGGAAA ACTTTGATCT CGCTTATGCG GAGCTGCATG AGATTGGGAG GGGACGATTC 
TCCATTGTAA AGAAATGCGT TCACAAAGCT ACCCGGAAAG ACGTGGCTGT AAAATTCATC 
AGTAAAAAAA TGAAGAAGAA GGAGCAAGCA GCCCACGAGG CAGCTCTGTT ACAGCACTTA 
CAGCATCCTC AGTACATCAC TATCCATGAT ACCTATGAGT CTCCTACTTC TTACATCCTA 
GTCCTGGAGC TGATGGATGA TGGTCGTCTC CTAGATTATC TAATGAACCA TGATGAACTT 
ATGGAGGAAA AAGTAGCTTT CTACATAAGA GACACAATGG AAGCCTTGCA GTATCTTCAC 
AATTGCCGAG TGGCTCATTT AGATATAAAG CCAGAGAATC TGCTCATCGA TTTAAGGATC 
CCAGTTCCTC GTGTCAAGAT CATTGATTTG GAAGATGCAG TTCAAATCAC AGGCCATTAC 
CACGTCCACC ATCTCCTTGG AAACCCAGAG TTTGCAGCCC CTGAAGTTAT CCAGGGCCTC 
CCCGTGTCCC TGAGCACAGA CATCTGGAGC ATCGGGGTCC TCACCTACGT CATGCTGAGC 
GGTGTCTCCC CGTTCCTGGA TGAAAGCAAA GAGGAGACGT GTATCAATGT GTGCAGAGTC 
GATTTCAGCT TCCCGCCCGA GTACTTCTCT GATGTGAGCC ATGCTGCTAG GGATTTCATC 
AACGTCATCC TCCAGGAAGA CTCCAGGAGG AGGCCGACAG CAGCCACTTG TTTACAGCAC 
CCATGGCTGC AGCCACACAA TGGCAGCTAC TCCAAAATCC CGCTGGATAC CTCCCGCTTG 
GCATCCTTCA TTGAGCGCCG CAGACACCAG TACGACGTGC ACCCCGTCCC CGGTGTCAAG 
AGTTTCCTGA TGAGCAGGCT GAACCCCGGG ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145464.1
CDS	244..9159
Translation	MNPTEGAAEEGAVPDSEVDAFFRTGSFRNDGLKAADVLPILKEKVAFVSGGRDKRGGPVLTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLAKLVDPSQLTDEFEGSLDYNHDEWIELRVSLEEFFNSAIHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVIKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKELLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAVSNNKTPHNSSISHIESVLQQLDEAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDIDLATQVQELLEFLHEKQHELELNADQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEVLLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEIDHVIPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPGVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHNSTGESAEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKRYRDFSLRMGKYRYSLEKALGINTEDNKDLELDIIPASLSDREVKLRDANHEVNEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPAGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSSQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDSFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASSIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKQRNNSRRDGVDDADSQGDGSSQPDTISIASRTSQNTVDSDKLSGGCELTVVLQDFTAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPSGKEAYSVSSNENGGKSDSVANLQPQPSLNSIQSSPGPKRSTNTLKKWLTSPVRRLNSGKAESNIKKQKKVRDGRKSFDLGSPKTGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSSLLAARQSSSDVPTAADLVSAIEQLVKSKLTLEGGSYRGSLKDATGCLNEGMAPSTPPRNLEEEQKAKAMRGRMFVLNELVQTEKDYVKDLGTVVEGFMKRIEEKGVSEDMKGKDKIVFGNIHQIYDWHKDFFLAELEKCLQEPDRLAQLFIKHERRLHMYVVYCQNKPRSEYIVAEYEAYFEEVQQEINQRLTISDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSEIEKAVELMCLVPKRCNDMMNLGRLQGFEGKLTAQGKLLQQDTFYVTEHDSGVQSRPKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVIEENVDGDPCKFALMNRETSEKVVLQAANPEIQQAWVQDINQVLDTQRDFLNALQSPIEYQRKESSTAVLRPQAGRVAQPNSRPYSSVPVGSEKPLKATSRNPSLPPLKISTSNGSTGYEQPGDKYEQSKTDLGGCNGTSSMVVIKDYYAVKEDEICVNQGEVVQILAINQQNMFLVYQPANDHSPAAEGWIPGNILAPLTKPTTDNSDGSIKKSCSWHTLRMRKRAEKESSGKNEANGPRKSKDILGNKVSVKETNSSEESECDDLDPNTSMEIINPNFIQEVAPEFLVLLVDITCLLGDTVMLQCKVCGRPKPTITWKGPDQNILDNDNSTATYTVSSCDSGELMLKICNVMPQDSGIYTCVATNEHGTASTSATIKVQGVPAAPNRPIAQERSCTSVILRWLPPSSTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCQYQFRVSASNPWGISLPSDPSEFVRLPEYDSAADGATISWKENFDLAYAELHEIGRGRFSIVKKCVHKATRKDVAVKFISKKMKKKEQAAHEAALLQHLQHPQYITIHDTYESPTSYILVLELMDDGRLLDYLMNHDELMEEKVAFYIRDTMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKIIDLEDAVQITGHYHVHHLLGNPEFAAPEVIQGLPVSLSTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPPEYFSDVSHAARDFINVILQEDSRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLASFIERRRHQYDVHPVPGVKSFLMSRLNPGT

Target ORF information:

RefSeq Version	XM_015289978.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289978.2

ORF Insert Sequence:

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGTTGT CTGGAGGGTG TGAACTAACC 
GTGGTGCTCC AGGACTTCAC GGCGGGCCAC AGCAGCGAGC TGAGCATTCA GGTGGGGCAG 
ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA AGGCCAGGCT GGTGCTTGGT GCGGACCACG 
GAGCGGAGCC CACCTCAGGA AGGCCTGGTC CCCAGCAGCG CTCTCTGCAT TTCCCATTCC 
CGCAGCAGCG TGGAGATGGA TTGCTTCTTC CCTTCAGGAA AAGAGGCATA TTCAGTCTCT 
TCCAATGAAA ACGGGGGCAA GTCCGATTCA GTGGCCAACT TACAGCCCCA GCCATCCCTC 
AACTCCATCC AGAGCTCTCC CGGCCCCAAG CGCTCGACCA ACACGCTGAA GAAATGGCTG 
ACGAGCCCCG TGCGCCGGCT GAACAGCGGG AAGGCCGAGA GCAACATCAA GAAGCAAAAG 
AAGGTGCGAG ATGGCAGGAA GAGCTTTGAC CTCGGCTCGC CGAAGACTGG AGATGAAACC 
ACTCCTCAAG GAGACAGCGC GGATGAGAAG AGCAAGAAGG GTTGGGGAGA AGATGAGCCA 
GATGAAGAGT CTCACACACC TCTTCCTCCT CCTATGAAGA TTTTTGACAA TGATCCAACC 
CAAGATGAGA TGTCCTCTTC TTTGCTAGCT GCTCGGCAGA GCTCCTCCGA TGTCCCAACT 
GCTGCGGATC TTGTCAGTGC AATAGAACAG TTGGTCAAAA GTAAGCTGAC CCTTGAAGGA 
GGATCTTACC GAGGAAGCTT AAAAGATGCC ACAGGCTGCT TGAATGAAGG AATGGCGCCT 
TCAACTCCCC CAAGAAACCT TGAAGAGGAA CAAAAAGCCA AAGCAATGAG AGGCAGGATG 
TTTGTCTTAA ATGAACTGGT CCAGACTGAA AAAGACTACG TCAAAGACTT GGGAACCGTT 
GTGGAGGGCT TCATGAAGAG AATAGAAGAA AAGGGAGTTT CCGAAGATAT GAAAGGGAAA 
GACAAGATAG TGTTTGGCAA TATTCACCAG ATCTATGACT GGCACAAAGA CTTTTTCCTG 
GCTGAACTGG AAAAATGTCT TCAGGAGCCT GACCGTTTAG CACAGCTTTT TATTAAACAT 
GAGCGGCGAT TACACATGTA TGTGGTGTAC TGCCAAAACA AGCCACGATC TGAATATATA 
GTAGCTGAAT ACGAGGCATA TTTTGAGGAG GTTCAACAGG AAATAAACCA ACGGCTGACC 
ATCAGTGACT TTCTGATCAA ACCCATTCAG AGAATAACTA AATATCAGCT TCTTCTCAAG 
GACTTCCTGA GGTACAGTGA AAAAGCTGGT CTGGAGTGCT CTGAAATAGA GAAAGCAGTT 
GAGTTAATGT GCCTTGTACC AAAACGTTGC AATGATATGA TGAACCTGGG TCGCCTCCAA 
GGCTTTGAGG GTAAGCTGAC AGCCCAGGGC AAGCTGCTGC AGCAGGACAC CTTCTACGTG 
ACAGAGCATG ACTCCGGAGT TCAGTCCCGA CCAAAGGAAC GTAGGGTGTT TCTCTTTGAG 
CAAATAGTTA TCTTCAGCGA GCTGCTTAGG AAAGGGTCTT TAACACCAGG CTACATGTTC 
AAGAGAAGCA TAAAGATGAA CTACCTTGTC ATTGAAGAAA ATGTGGATGG TGATCCCTGC 
AAGTTTGCTC TAATGAATCG GGAAACATCT GAGAAAGTTG TTTTACAGGC AGCTAATCCA 
GAAATTCAGC AGGCTTGGGT ACAGGACATC AACCAGGTCC TGGACACACA AAGGGATTTC 
CTAAATGCAC TGCAGTCTCC CATAGAGTAC CAACGCAAAG AAAGTAGCAC AGCAGTGCTG 
AGGCCCCAGG CTGGTAGGGT CGCTCAGCCC AACAGCAGAC CCTATTCTTC TGTTCCAGTA 
GGGTCTGAGA AGCCTTTGAA GGCTACAAGC CGTAACCCAT CTCTCCCACC CCTGAAGATA 
TCTACCTCCA ACGGCAGTAC AGGGTATGAA CAGCCCGGAG ACAAGTATGA ACAAAGCAAG 
ACTGATTTGG GAGGGTGCAA TGGGACATCT TCCATGGTGG TGATTAAAGA TTACTATGCA 
GTGAAGGAGG ACGAGATCTG TGTGAATCAA GGAGAGGTGG TTCAGATCCT TGCTATCAAC 
CAGCAAAACA TGTTCCTGGT GTACCAGCCT GCAAATGACC ACTCTCCAGC TGCTGAAGGA 
TGGATCCCTG GCAATATCTT GGCTCCCCTC ACTAAACCCA CTACAGATAA TAGCGACGGA 
AGCATCAAGA AGTCATGTTC ATGGCATACC CTGCGCATGA GAAAGCGGGC GGAAAAGGAG 
AGCAGTGGCA AAAATGAAGC CAATGGGCCA CGTAAATCCA AGGATATTCT GGGGAACAAA 
GTCTCTGTTA AAGAGACAAA CAGCTCAGAG GAATCAGAGT GTGATGACCT TGACCCCAAT 
ACTAGCATGG AGATAATCAA CCCCAATTTC ATTCAGGAAG TGGCTCCAGA GTTCCTTGTG 
CTGTTGGTGG ATATCACCTG CCTGCTTGGC GACACTGTGA TGCTGCAGTG CAAAGTCTGC 
GGCCGGCCCA AACCAACAAT AACCTGGAAA GGACCTGATC AAAACATTCT TGACAATGAC 
AACAGCACGG CCACATACAC GGTGTCATCC TGTGACTCTG GAGAGCTCAT GCTGAAGATC 
TGCAATGTGA TGCCTCAGGA CAGTGGCATT TACACTTGTG TGGCAACTAA CGAGCATGGA 
ACGGCATCAA CCTCAGCCAC AATCAAAGTG CAAGGTGTAC CGGCTGCCCC AAATCGCCCC 
ATTGCTCAGG AGAGAAGCTG CACCTCTGTG ATCCTTCGCT GGCTGCCTCC ATCCAGTACA 
GGCAATTGCA CCATTTCTGG CTACACAGTG GAGTACAGAG AAGAAGGCTC ACAGGTCTGG 
CAGCAGTCAG TGGCCTCCAC ACTGGACACT TACCTTGTCA TTGAAGACCT GTCCCCAGGC 
TGCCAGTATC AGTTCCGTGT GAGTGCCAGC AACCCCTGGG GGATCAGCTT GCCCAGTGAC 
CCATCTGAAT TCGTGAGGCT TCCAGAGTAT GATTCTGCTG CTGATGGTGC CACTATTTCG 
TGGAAGGAAA ACTTTGATCT CGCTTATGCG GAGCTGCATG AGATTGGGAG GGGACGATTC 
TCCATTGTAA AGAAATGCGT TCACAAAGCT ACCCGGAAAG ACGTGGCTGT AAAATTCATC 
AGTAAAAAAA TGAAGAAGAA GGAGCAAGCA GCCCACGAGG CAGCTCTGTT ACAGCACTTA 
CAGCATCCTC AGTACATCAC TATCCATGAT ACCTATGAGT CTCCTACTTC TTACATCCTA 
GTCCTGGAGC TGATGGATGA TGGTCGTCTC CTAGATTATC TAATGAACCA TGATGAACTT 
ATGGAGGAAA AAGTAGCTTT CTACATAAGA GACACAATGG AAGCCTTGCA GTATCTTCAC 
AATTGCCGAG TGGCTCATTT AGATATAAAG CCAGAGAATC TGCTCATCGA TTTAAGGATC 
CCAGTTCCTC GTGTCAAGAT CATTGATTTG GAAGATGCAG TTCAAATCAC AGGCCATTAC 
CACGTCCACC ATCTCCTTGG AAACCCAGAG TTTGCAGCCC CTGAAGTTAT CCAGGGCCTC 
CCCGTGTCCC TGAGCACAGA CATCTGGAGC ATCGGGGTCC TCACCTACGT CATGCTGAGC 
GGTGTCTCCC CGTTCCTGGA TGAAAGCAAA GAGGAGACGT GTATCAATGT GTGCAGAGTC 
GATTTCAGCT TCCCGCCCGA GTACTTCTCT GATGTGAGCC ATGCTGCTAG GGATTTCATC 
AACGTCATCC TCCAGGAAGA CTCCAGGAGG AGGCCGACAG CAGCCACTTG TTTACAGCAC 
CCATGGCTGC AGCCACACAA TGGCAGCTAC TCCAAAATCC CGCTGGATAC CTCCCGCTTG 
GCATCCTTCA TTGAGCGCCG CAGACACCAG TACGACGTGC ACCCCGTCCC CGGTGTCAAG 
AGTTTCCTGA TGAGCAGGCT GAACCCCGGG ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KALRN ( XM_015289978.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145464.1 Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X4, mRNA.

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CloneID	OGa38674
Clone ID Related Accession (Same CDS sequence)	XM_015289978.2 , XM_015289978.1
Accession Version	XM_015289978.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	kalirin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGTTGT CTGGAGGGTG TGAACTAACC 
GTGGTGCTCC AGGACTTCAC GGCGGGCCAC AGCAGCGAGC TGAGCATTCA GGTGGGGCAG 
ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA AGGCCAGGCT GGTGCTTGGT GCGGACCACG 
GAGCGGAGCC CACCTCAGGA AGGCCTGGTC CCCAGCAGCG CTCTCTGCAT TTCCCATTCC 
CGCAGCAGCG TGGAGATGGA TTGCTTCTTC CCTTCAGGAA AAGAGGCATA TTCAGTCTCT 
TCCAATGAAA ACGGGGGCAA GTCCGATTCA GTGGCCAACT TACAGCCCCA GCCATCCCTC 
AACTCCATCC AGAGCTCTCC CGGCCCCAAG CGCTCGACCA ACACGCTGAA GAAATGGCTG 
ACGAGCCCCG TGCGCCGGCT GAACAGCGGG AAGGCCGAGA GCAACATCAA GAAGCAAAAG 
AAGGTGCGAG ATGGCAGGAA GAGCTTTGAC CTCGGCTCGC CGAAGACTGG AGATGAAACC 
ACTCCTCAAG GAGACAGCGC GGATGAGAAG AGCAAGAAGG GTTGGGGAGA AGATGAGCCA 
GATGAAGAGT CTCACACACC TCTTCCTCCT CCTATGAAGA TTTTTGACAA TGATCCAACC 
CAAGATGAGA TGTCCTCTTC TTTGCTAGCT GCTCGGCAGA GCTCCTCCGA TGTCCCAACT 
GCTGCGGATC TTGTCAGTGC AATAGAACAG TTGGTCAAAA GTAAGCTGAC CCTTGAAGGA 
GGATCTTACC GAGGAAGCTT AAAAGATGCC ACAGGCTGCT TGAATGAAGG AATGGCGCCT 
TCAACTCCCC CAAGAAACCT TGAAGAGGAA CAAAAAGCCA AAGCAATGAG AGGCAGGATG 
TTTGTCTTAA ATGAACTGGT CCAGACTGAA AAAGACTACG TCAAAGACTT GGGAACCGTT 
GTGGAGGGCT TCATGAAGAG AATAGAAGAA AAGGGAGTTT CCGAAGATAT GAAAGGGAAA 
GACAAGATAG TGTTTGGCAA TATTCACCAG ATCTATGACT GGCACAAAGA CTTTTTCCTG 
GCTGAACTGG AAAAATGTCT TCAGGAGCCT GACCGTTTAG CACAGCTTTT TATTAAACAT 
GAGCGGCGAT TACACATGTA TGTGGTGTAC TGCCAAAACA AGCCACGATC TGAATATATA 
GTAGCTGAAT ACGAGGCATA TTTTGAGGAG GTTCAACAGG AAATAAACCA ACGGCTGACC 
ATCAGTGACT TTCTGATCAA ACCCATTCAG AGAATAACTA AATATCAGCT TCTTCTCAAG 
GACTTCCTGA GGTACAGTGA AAAAGCTGGT CTGGAGTGCT CTGAAATAGA GAAAGCAGTT 
GAGTTAATGT GCCTTGTACC AAAACGTTGC AATGATATGA TGAACCTGGG TCGCCTCCAA 
GGCTTTGAGG GTAAGCTGAC AGCCCAGGGC AAGCTGCTGC AGCAGGACAC CTTCTACGTG 
ACAGAGCATG ACTCCGGAGT TCAGTCCCGA CCAAAGGAAC GTAGGGTGTT TCTCTTTGAG 
CAAATAGTTA TCTTCAGCGA GCTGCTTAGG AAAGGGTCTT TAACACCAGG CTACATGTTC 
AAGAGAAGCA TAAAGATGAA CTACCTTGTC ATTGAAGAAA ATGTGGATGG TGATCCCTGC 
AAGTTTGCTC TAATGAATCG GGAAACATCT GAGAAAGTTG TTTTACAGGC AGCTAATCCA 
GAAATTCAGC AGGCTTGGGT ACAGGACATC AACCAGGTCC TGGACACACA AAGGGATTTC 
CTAAATGCAC TGCAGTCTCC CATAGAGTAC CAACGCAAAG AAAGTAGCAC AGCAGTGCTG 
AGGCCCCAGG CTGGTAGGGT CGCTCAGCCC AACAGCAGAC CCTATTCTTC TGTTCCAGTA 
GGGTCTGAGA AGCCTTTGAA GGCTACAAGC CGTAACCCAT CTCTCCCACC CCTGAAGATA 
TCTACCTCCA ACGGCAGTAC AGGGTATGAA CAGCCCGGAG ACAAGTATGA ACAAAGCAAG 
ACTGATTTGG GAGGGTGCAA TGGGACATCT TCCATGGTGG TGATTAAAGA TTACTATGCA 
GTGAAGGAGG ACGAGATCTG TGTGAATCAA GGAGAGGTGG TTCAGATCCT TGCTATCAAC 
CAGCAAAACA TGTTCCTGGT GTACCAGCCT GCAAATGACC ACTCTCCAGC TGCTGAAGGA 
TGGATCCCTG GCAATATCTT GGCTCCCCTC ACTAAACCCA CTACAGATAA TAGCGACGGA 
AGCATCAAGA AGTCATGTTC ATGGCATACC CTGCGCATGA GAAAGCGGGC GGAAAAGGAG 
AGCAGTGGCA AAAATGAAGC CAATGGGCCA CGTAAATCCA AGGATATTCT GGGGAACAAA 
GTCTCTGTTA AAGAGACAAA CAGCTCAGAG GAATCAGAGT GTGATGACCT TGACCCCAAT 
ACTAGCATGG AGATAATCAA CCCCAATTTC ATTCAGGAAG TGGCTCCAGA GTTCCTTGTG 
CTGTTGGTGG ATATCACCTG CCTGCTTGGC GACACTGTGA TGCTGCAGTG CAAAGTCTGC 
GGCCGGCCCA AACCAACAAT AACCTGGAAA GGACCTGATC AAAACATTCT TGACAATGAC 
AACAGCACGG CCACATACAC GGTGTCATCC TGTGACTCTG GAGAGCTCAT GCTGAAGATC 
TGCAATGTGA TGCCTCAGGA CAGTGGCATT TACACTTGTG TGGCAACTAA CGAGCATGGA 
ACGGCATCAA CCTCAGCCAC AATCAAAGTG CAAGGTGTAC CGGCTGCCCC AAATCGCCCC 
ATTGCTCAGG AGAGAAGCTG CACCTCTGTG ATCCTTCGCT GGCTGCCTCC ATCCAGTACA 
GGCAATTGCA CCATTTCTGG CTACACAGTG GAGTACAGAG AAGAAGGCTC ACAGGTCTGG 
CAGCAGTCAG TGGCCTCCAC ACTGGACACT TACCTTGTCA TTGAAGACCT GTCCCCAGGC 
TGCCAGTATC AGTTCCGTGT GAGTGCCAGC AACCCCTGGG GGATCAGCTT GCCCAGTGAC 
CCATCTGAAT TCGTGAGGCT TCCAGAGTAT GATTCTGCTG CTGATGGTGC CACTATTTCG 
TGGAAGGAAA ACTTTGATCT CGCTTATGCG GAGCTGCATG AGATTGGGAG GGGACGATTC 
TCCATTGTAA AGAAATGCGT TCACAAAGCT ACCCGGAAAG ACGTGGCTGT AAAATTCATC 
AGTAAAAAAA TGAAGAAGAA GGAGCAAGCA GCCCACGAGG CAGCTCTGTT ACAGCACTTA 
CAGCATCCTC AGTACATCAC TATCCATGAT ACCTATGAGT CTCCTACTTC TTACATCCTA 
GTCCTGGAGC TGATGGATGA TGGTCGTCTC CTAGATTATC TAATGAACCA TGATGAACTT 
ATGGAGGAAA AAGTAGCTTT CTACATAAGA GACACAATGG AAGCCTTGCA GTATCTTCAC 
AATTGCCGAG TGGCTCATTT AGATATAAAG CCAGAGAATC TGCTCATCGA TTTAAGGATC 
CCAGTTCCTC GTGTCAAGAT CATTGATTTG GAAGATGCAG TTCAAATCAC AGGCCATTAC 
CACGTCCACC ATCTCCTTGG AAACCCAGAG TTTGCAGCCC CTGAAGTTAT CCAGGGCCTC 
CCCGTGTCCC TGAGCACAGA CATCTGGAGC ATCGGGGTCC TCACCTACGT CATGCTGAGC 
GGTGTCTCCC CGTTCCTGGA TGAAAGCAAA GAGGAGACGT GTATCAATGT GTGCAGAGTC 
GATTTCAGCT TCCCGCCCGA GTACTTCTCT GATGTGAGCC ATGCTGCTAG GGATTTCATC 
AACGTCATCC TCCAGGAAGA CTCCAGGAGG AGGCCGACAG CAGCCACTTG TTTACAGCAC 
CCATGGCTGC AGCCACACAA TGGCAGCTAC TCCAAAATCC CGCTGGATAC CTCCCGCTTG 
GCATCCTTCA TTGAGCGCCG CAGACACCAG TACGACGTGC ACCCCGTCCC CGGTGTCAAG 
AGTTTCCTGA TGAGCAGGCT GAACCCCGGG ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145464.1
CDS	234..9149
Translation	MNPTEGAAEEGAVPDSEVDAFFRTGSFRNDGLKAADVLPILKEKVAFVSGGRDKRGGPVLTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLAKLVDPSQLTDEFEGSLDYNHDEWIELRVSLEEFFNSAIHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVIKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKELLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAVSNNKTPHNSSISHIESVLQQLDEAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDIDLATQVQELLEFLHEKQHELELNADQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEVLLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEIDHVIPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPGVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHNSTGESAEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKRYRDFSLRMGKYRYSLEKALGINTEDNKDLELDIIPASLSDREVKLRDANHEVNEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPAGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSSQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDSFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASSIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKQRNNSRRDGVDDADSQGDGSSQPDTISIASRTSQNTVDSDKLSGGCELTVVLQDFTAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPSGKEAYSVSSNENGGKSDSVANLQPQPSLNSIQSSPGPKRSTNTLKKWLTSPVRRLNSGKAESNIKKQKKVRDGRKSFDLGSPKTGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSSLLAARQSSSDVPTAADLVSAIEQLVKSKLTLEGGSYRGSLKDATGCLNEGMAPSTPPRNLEEEQKAKAMRGRMFVLNELVQTEKDYVKDLGTVVEGFMKRIEEKGVSEDMKGKDKIVFGNIHQIYDWHKDFFLAELEKCLQEPDRLAQLFIKHERRLHMYVVYCQNKPRSEYIVAEYEAYFEEVQQEINQRLTISDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSEIEKAVELMCLVPKRCNDMMNLGRLQGFEGKLTAQGKLLQQDTFYVTEHDSGVQSRPKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVIEENVDGDPCKFALMNRETSEKVVLQAANPEIQQAWVQDINQVLDTQRDFLNALQSPIEYQRKESSTAVLRPQAGRVAQPNSRPYSSVPVGSEKPLKATSRNPSLPPLKISTSNGSTGYEQPGDKYEQSKTDLGGCNGTSSMVVIKDYYAVKEDEICVNQGEVVQILAINQQNMFLVYQPANDHSPAAEGWIPGNILAPLTKPTTDNSDGSIKKSCSWHTLRMRKRAEKESSGKNEANGPRKSKDILGNKVSVKETNSSEESECDDLDPNTSMEIINPNFIQEVAPEFLVLLVDITCLLGDTVMLQCKVCGRPKPTITWKGPDQNILDNDNSTATYTVSSCDSGELMLKICNVMPQDSGIYTCVATNEHGTASTSATIKVQGVPAAPNRPIAQERSCTSVILRWLPPSSTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCQYQFRVSASNPWGISLPSDPSEFVRLPEYDSAADGATISWKENFDLAYAELHEIGRGRFSIVKKCVHKATRKDVAVKFISKKMKKKEQAAHEAALLQHLQHPQYITIHDTYESPTSYILVLELMDDGRLLDYLMNHDELMEEKVAFYIRDTMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKIIDLEDAVQITGHYHVHHLLGNPEFAAPEVIQGLPVSLSTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPPEYFSDVSHAARDFINVILQEDSRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLASFIERRRHQYDVHPVPGVKSFLMSRLNPGT

Target ORF information:

RefSeq Version	XM_015289978.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289978.1

ORF Insert Sequence:

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGTTGT CTGGAGGGTG TGAACTAACC 
GTGGTGCTCC AGGACTTCAC GGCGGGCCAC AGCAGCGAGC TGAGCATTCA GGTGGGGCAG 
ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA AGGCCAGGCT GGTGCTTGGT GCGGACCACG 
GAGCGGAGCC CACCTCAGGA AGGCCTGGTC CCCAGCAGCG CTCTCTGCAT TTCCCATTCC 
CGCAGCAGCG TGGAGATGGA TTGCTTCTTC CCTTCAGGAA AAGAGGCATA TTCAGTCTCT 
TCCAATGAAA ACGGGGGCAA GTCCGATTCA GTGGCCAACT TACAGCCCCA GCCATCCCTC 
AACTCCATCC AGAGCTCTCC CGGCCCCAAG CGCTCGACCA ACACGCTGAA GAAATGGCTG 
ACGAGCCCCG TGCGCCGGCT GAACAGCGGG AAGGCCGAGA GCAACATCAA GAAGCAAAAG 
AAGGTGCGAG ATGGCAGGAA GAGCTTTGAC CTCGGCTCGC CGAAGACTGG AGATGAAACC 
ACTCCTCAAG GAGACAGCGC GGATGAGAAG AGCAAGAAGG GTTGGGGAGA AGATGAGCCA 
GATGAAGAGT CTCACACACC TCTTCCTCCT CCTATGAAGA TTTTTGACAA TGATCCAACC 
CAAGATGAGA TGTCCTCTTC TTTGCTAGCT GCTCGGCAGA GCTCCTCCGA TGTCCCAACT 
GCTGCGGATC TTGTCAGTGC AATAGAACAG TTGGTCAAAA GTAAGCTGAC CCTTGAAGGA 
GGATCTTACC GAGGAAGCTT AAAAGATGCC ACAGGCTGCT TGAATGAAGG AATGGCGCCT 
TCAACTCCCC CAAGAAACCT TGAAGAGGAA CAAAAAGCCA AAGCAATGAG AGGCAGGATG 
TTTGTCTTAA ATGAACTGGT CCAGACTGAA AAAGACTACG TCAAAGACTT GGGAACCGTT 
GTGGAGGGCT TCATGAAGAG AATAGAAGAA AAGGGAGTTT CCGAAGATAT GAAAGGGAAA 
GACAAGATAG TGTTTGGCAA TATTCACCAG ATCTATGACT GGCACAAAGA CTTTTTCCTG 
GCTGAACTGG AAAAATGTCT TCAGGAGCCT GACCGTTTAG CACAGCTTTT TATTAAACAT 
GAGCGGCGAT TACACATGTA TGTGGTGTAC TGCCAAAACA AGCCACGATC TGAATATATA 
GTAGCTGAAT ACGAGGCATA TTTTGAGGAG GTTCAACAGG AAATAAACCA ACGGCTGACC 
ATCAGTGACT TTCTGATCAA ACCCATTCAG AGAATAACTA AATATCAGCT TCTTCTCAAG 
GACTTCCTGA GGTACAGTGA AAAAGCTGGT CTGGAGTGCT CTGAAATAGA GAAAGCAGTT 
GAGTTAATGT GCCTTGTACC AAAACGTTGC AATGATATGA TGAACCTGGG TCGCCTCCAA 
GGCTTTGAGG GTAAGCTGAC AGCCCAGGGC AAGCTGCTGC AGCAGGACAC CTTCTACGTG 
ACAGAGCATG ACTCCGGAGT TCAGTCCCGA CCAAAGGAAC GTAGGGTGTT TCTCTTTGAG 
CAAATAGTTA TCTTCAGCGA GCTGCTTAGG AAAGGGTCTT TAACACCAGG CTACATGTTC 
AAGAGAAGCA TAAAGATGAA CTACCTTGTC ATTGAAGAAA ATGTGGATGG TGATCCCTGC 
AAGTTTGCTC TAATGAATCG GGAAACATCT GAGAAAGTTG TTTTACAGGC AGCTAATCCA 
GAAATTCAGC AGGCTTGGGT ACAGGACATC AACCAGGTCC TGGACACACA AAGGGATTTC 
CTAAATGCAC TGCAGTCTCC CATAGAGTAC CAACGCAAAG AAAGTAGCAC AGCAGTGCTG 
AGGCCCCAGG CTGGTAGGGT CGCTCAGCCC AACAGCAGAC CCTATTCTTC TGTTCCAGTA 
GGGTCTGAGA AGCCTTTGAA GGCTACAAGC CGTAACCCAT CTCTCCCACC CCTGAAGATA 
TCTACCTCCA ACGGCAGTAC AGGGTATGAA CAGCCCGGAG ACAAGTATGA ACAAAGCAAG 
ACTGATTTGG GAGGGTGCAA TGGGACATCT TCCATGGTGG TGATTAAAGA TTACTATGCA 
GTGAAGGAGG ACGAGATCTG TGTGAATCAA GGAGAGGTGG TTCAGATCCT TGCTATCAAC 
CAGCAAAACA TGTTCCTGGT GTACCAGCCT GCAAATGACC ACTCTCCAGC TGCTGAAGGA 
TGGATCCCTG GCAATATCTT GGCTCCCCTC ACTAAACCCA CTACAGATAA TAGCGACGGA 
AGCATCAAGA AGTCATGTTC ATGGCATACC CTGCGCATGA GAAAGCGGGC GGAAAAGGAG 
AGCAGTGGCA AAAATGAAGC CAATGGGCCA CGTAAATCCA AGGATATTCT GGGGAACAAA 
GTCTCTGTTA AAGAGACAAA CAGCTCAGAG GAATCAGAGT GTGATGACCT TGACCCCAAT 
ACTAGCATGG AGATAATCAA CCCCAATTTC ATTCAGGAAG TGGCTCCAGA GTTCCTTGTG 
CTGTTGGTGG ATATCACCTG CCTGCTTGGC GACACTGTGA TGCTGCAGTG CAAAGTCTGC 
GGCCGGCCCA AACCAACAAT AACCTGGAAA GGACCTGATC AAAACATTCT TGACAATGAC 
AACAGCACGG CCACATACAC GGTGTCATCC TGTGACTCTG GAGAGCTCAT GCTGAAGATC 
TGCAATGTGA TGCCTCAGGA CAGTGGCATT TACACTTGTG TGGCAACTAA CGAGCATGGA 
ACGGCATCAA CCTCAGCCAC AATCAAAGTG CAAGGTGTAC CGGCTGCCCC AAATCGCCCC 
ATTGCTCAGG AGAGAAGCTG CACCTCTGTG ATCCTTCGCT GGCTGCCTCC ATCCAGTACA 
GGCAATTGCA CCATTTCTGG CTACACAGTG GAGTACAGAG AAGAAGGCTC ACAGGTCTGG 
CAGCAGTCAG TGGCCTCCAC ACTGGACACT TACCTTGTCA TTGAAGACCT GTCCCCAGGC 
TGCCAGTATC AGTTCCGTGT GAGTGCCAGC AACCCCTGGG GGATCAGCTT GCCCAGTGAC 
CCATCTGAAT TCGTGAGGCT TCCAGAGTAT GATTCTGCTG CTGATGGTGC CACTATTTCG 
TGGAAGGAAA ACTTTGATCT CGCTTATGCG GAGCTGCATG AGATTGGGAG GGGACGATTC 
TCCATTGTAA AGAAATGCGT TCACAAAGCT ACCCGGAAAG ACGTGGCTGT AAAATTCATC 
AGTAAAAAAA TGAAGAAGAA GGAGCAAGCA GCCCACGAGG CAGCTCTGTT ACAGCACTTA 
CAGCATCCTC AGTACATCAC TATCCATGAT ACCTATGAGT CTCCTACTTC TTACATCCTA 
GTCCTGGAGC TGATGGATGA TGGTCGTCTC CTAGATTATC TAATGAACCA TGATGAACTT 
ATGGAGGAAA AAGTAGCTTT CTACATAAGA GACACAATGG AAGCCTTGCA GTATCTTCAC 
AATTGCCGAG TGGCTCATTT AGATATAAAG CCAGAGAATC TGCTCATCGA TTTAAGGATC 
CCAGTTCCTC GTGTCAAGAT CATTGATTTG GAAGATGCAG TTCAAATCAC AGGCCATTAC 
CACGTCCACC ATCTCCTTGG AAACCCAGAG TTTGCAGCCC CTGAAGTTAT CCAGGGCCTC 
CCCGTGTCCC TGAGCACAGA CATCTGGAGC ATCGGGGTCC TCACCTACGT CATGCTGAGC 
GGTGTCTCCC CGTTCCTGGA TGAAAGCAAA GAGGAGACGT GTATCAATGT GTGCAGAGTC 
GATTTCAGCT TCCCGCCCGA GTACTTCTCT GATGTGAGCC ATGCTGCTAG GGATTTCATC 
AACGTCATCC TCCAGGAAGA CTCCAGGAGG AGGCCGACAG CAGCCACTTG TTTACAGCAC 
CCATGGCTGC AGCCACACAA TGGCAGCTAC TCCAAAATCC CGCTGGATAC CTCCCGCTTG 
GCATCCTTCA TTGAGCGCCG CAGACACCAG TACGACGTGC ACCCCGTCCC CGGTGTCAAG 
AGTTTCCTGA TGAGCAGGCT GAACCCCGGG ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KALRN ( XM_015289979.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145465.1 Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X5, mRNA.

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CloneID	OGa38675
Clone ID Related Accession (Same CDS sequence)	XM_015289979.1
Accession Version	XM_015289979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	kalirin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG AAGACACACC AGAGTGCCCT GCAGGTCCAG 
CAGAAAGCCG AAGTGCTGCT GCAGGCTGGG CATTATGATG CTGATGCCAT CAGAGAATGC 
GCTGAAAAGG TGGCCCTGCA CTGGCAGCAG CTGATGCTGA AGATGGAGGA CAGGCTCAAG 
CTGGTCAATG CCTCGGTGGC CTTCTACAAA ACCTCTGAGC AGGTGTGCAG TGTATTAGAG 
AGCCTGGAGC AAGAATACAG ACGTGATGAA GACTGGTGTG GAGGTCGAGA TAAACTTGGA 
CCAGCAGCTG AGATAGACCA CGTCATCCCT CTGATCAGCA AGCATCTGGA GCAGAAGGAG 
GCTTTTCTCA AGGCCTGCAC ACTGGCTCGA AGGAATGCTG AGGTATTCCT CAAGTACATC 
CACAGGAACA ATGTCAGCAT GCCCGGAGTC GCAAGCCATA CACGGGGACC TGAGCAGCAA 
GTGAAAGCCA TCCTGAGTGA GTTGCTGCAG AGGGAGAACC GGGTCCTTCA CTTCTGGACC 
CTGAAGAAGC GGAGGTTAGA TCAGTGCCAG CAGTACGTTG TATTTGAGCG CAGTGCCAAG 
CAGGCCCTGG ACTGGATCCA AGAGACAGGC GAATATTACC TCTCTACTCA CAACTCTACA 
GGGGAATCAG CAGAAGAAAC TCAGGAACTC CTGAAGGAAT ATGGGGAATT CAGAGTACCT 
GCGAAGCAAA CAAAGGAGAA AGTGAAGCTC CTCATCCAGC TGGCTGACAG CTTTGTAGAA 
AAAGGACATA TACACGCCAC TGAAATCAGG AAGTGGGTCA CCACCGTTGA CAAACGCTAC 
CGTGACTTCT CTCTCAGGAT GGGGAAATAC CGCTACTCCC TGGAGAAAGC CCTTGGAATC 
AATACAGAGG ATAACAAGGA CCTCGAACTA GATATTATTC CAGCAAGTCT TTCAGACCGG 
GAGGTAAAGC TGCGAGATGC AAATCACGAA GTCAATGAAG AAAAAAGAAA GTCTGCTAGA 
AAGAAAGAAT TCATTATGGC CGAGCTTCTT CAGACAGAGA AAGCTTATGT CAGGGACTTA 
CATGAATGTT TAGAGACTTA CCTTTGGGAA ATGACAAGTG GGGTGGAAGA AATACCCGCT 
GGGATCCTTA ATAAAGAACA CATCATCTTT GGCAATATTC AGGAGATATA TGACTTCCAT 
AACAACATTT TCCTGAAAGA ACTCGAGAAG TATGAGCAAC TGCCTGAGGA TGTGGGTCAC 
TGCTTTGTCA CCTGGGCAGA TAAATTTCAG ATGTATGTCA CCTACTGCAA AAACAAGCCT 
GACTCCAGCC AGCTCATCCT GGAACACGCA GGGACTTTCT TTGATGAAAT TCAGCAAAGG 
CATGGTCTGG CCAACTCTAT CTCTTCTTAT TTAATCAAGC CTGTCCAGAG GATCACAAAA 
TATCAACTCC TCCTGAAGGA ACTGCTTACC TGCTGTGAGG AAGGCAAAGG GGAACTCAAG 
GATGGTCTGG AAGTGATGCT CAGTGTCCCC AAGAAAGCCA ATGATGCAAT GCACGTCAGC 
ATGCTGGAAG GGTTTGATGA GAATCTAGAC GTCCAGGGAG AGCTGATTCT TCAGGATTCC 
TTCCAAGTGT GGGACCCCAA GTCTCTCATT CGGAAAGGCC GTGAGAGACA TTTGTTTCTC 
TTTGAAATCT CTTTGGTGTT TAGCAAAGAG ATCAAAGACT CTTCAGGACA CACAAAATAT 
GTTTACAAGA ACAAGTTACT GACCTCAGAA CTGGGAGTGA CTGAACATGT TGAAGGAGAT 
CCCTGTAAAT TTGCCTTGTG GTCTGGACGA ACACCATCCT CAGACAATAA AACAGTGCTG 
AAAGCATCGA GTATTGAAAC AAAACAGGAG TGGATCAAAA ATATCCGAGA AGTCATCCAA 
GAAAGAATTA TCCATCTGAA AGGAGCTCTG AAAGAGCCAA TTCAGCTCCC CAAGACACCA 
GCAAAGCAGC GAAACAACAG TAGAAGAGAT GGAGTAGATG ATGCAGACAG CCAAGGAGAT 
GGAAGCAGTC AACCTGACAC CATTTCCATT GCATCCAGGA CATCACAGAA TACAGTGGAC 
AGTGATAAGT TGTCTGGAGG GTGTGAACTA ACCGTGGTGC TCCAGGACTT CACGGCGGGC 
CACAGCAGCG AGCTGAGCAT TCAGGTGGGG CAGACAGTGG AGCTGCTGGA GAGGCCAAGT 
GAAAGGCCAG GCTGGTGCTT GGTGCGGACC ACGGAGCGGA GCCCACCTCA GGAAGGCCTG 
GTCCCCAGCA GCGCTCTCTG CATTTCCCAT TCCCGCAGCA GCGTGGAGAT GGATTGCTTC 
TTCCCTTCAG GAAAAGAGGC ATATTCAGTC TCTTCCAATG AAAACGGGGG CAAGTCCGAT 
TCAGTGGCCA ACTTACAGCC CCAGCCATCC CTCAACTCCA TCCAGAGCTC TCCCGGCCCC 
AAGCGCTCGA CCAACACGCT GAAGAAATGG CTGACGAGCC CCGTGCGCCG GCTGAACAGC 
GGGAAGGCCG AGAGCAACAT CAAGAAGCAA AAGAAGGTGC GAGATGGCAG GAAGAGCTTT 
GACCTCGGCT CGCCGAAGAC TGGAGATGAA ACCACTCCTC AAGGAGACAG CGCGGATGAG 
AAGAGCAAGA AGGGTTGGGG AGAAGATGAG CCAGATGAAG AGTCTCACAC ACCTCTTCCT 
CCTCCTATGA AGATTTTTGA CAATGATCCA ACCCAAGATG AGATGTCCTC TTCTTTGCTA 
GCTGCTCGGC AGAGCTCCTC CGATGTCCCA ACTGCTGCGG ATCTTGTCAG TGCAATAGAA 
CAGTTGGTCA AAAGTAAGCT GACCCTTGAA GGAGGATCTT ACCGAGGAAG CTTAAAAGAT 
GCCACAGGCT GCTTGAATGA AGGAATGGCG CCTTCAACTC CCCCAAGAAA CCTTGAAGAG 
GAACAAAAAG CCAAAGCAAT GAGAGGCAGG ATGTTTGTCT TAAATGAACT GGTCCAGACT 
GAAAAAGACT ACGTCAAAGA CTTGGGAACC GTTGTGGAGG GCTTCATGAA GAGAATAGAA 
GAAAAGGGAG TTTCCGAAGA TATGAAAGGG AAAGACAAGA TAGTGTTTGG CAATATTCAC 
CAGATCTATG ACTGGCACAA AGACTTTTTC CTGGCTGAAC TGGAAAAATG TCTTCAGGAG 
CCTGACCGTT TAGCACAGCT TTTTATTAAA CATGAGCGGC GATTACACAT GTATGTGGTG 
TACTGCCAAA ACAAGCCACG ATCTGAATAT ATAGTAGCTG AATACGAGGC ATATTTTGAG 
GAGGTTCAAC AGGAAATAAA CCAACGGCTG ACCATCAGTG ACTTTCTGAT CAAACCCATT 
CAGAGAATAA CTAAATATCA GCTTCTTCTC AAGGACTTCC TGAGGTACAG TGAAAAAGCT 
GGTCTGGAGT GCTCTGAAAT AGAGAAAGCA GTTGAGTTAA TGTGCCTTGT ACCAAAACGT 
TGCAATGATA TGATGAACCT GGGTCGCCTC CAAGGCTTTG AGGGTAAGCT GACAGCCCAG 
GGCAAGCTGC TGCAGCAGGA CACCTTCTAC GTGACAGAGC ATGACTCCGG AGTTCAGTCC 
CGACCAAAGG AACGTAGGGT GTTTCTCTTT GAGCAAATAG TTATCTTCAG CGAGCTGCTT 
AGGAAAGGGT CTTTAACACC AGGCTACATG TTCAAGAGAA GCATAAAGAT GAACTACCTT 
GTCATTGAAG AAAATGTGGA TGGTGATCCC TGCAAGTTTG CTCTAATGAA TCGGGAAACA 
TCTGAGAAAG TTGTTTTACA GGCAGCTAAT CCAGAAATTC AGCAGGCTTG GGTACAGGAC 
ATCAACCAGG TCCTGGACAC ACAAAGGGAT TTCCTAAATG CACTGCAGTC TCCCATAGAG 
TACCAACGCA AAGAAAGTAG CACAGCAGTG CTGAGGCCCC AGGCTGGTAG GGTCGCTCAG 
CCCAACAGCA GACCCTATTC TTCTGTTCCA GTAGGGTCTG AGAAGCCTTT GAAGGCTACA 
AGCCGTAACC CATCTCTCCC ACCCCTGAAG ATATCTACCT CCAACGGCAG TACAGGGTAT 
GAACAGCCCG GAGACAAGTA TGAACAAAGC AAGACTGATT TGGGAGGGTG CAATGGGACA 
TCTTCCATGG TGGTGATTAA AGATTACTAT GCAGTGAAGG AGGACGAGAT CTGTGTGAAT 
CAAGGAGAGG TGGTTCAGAT CCTTGCTATC AACCAGCAAA ACATGTTCCT GGTGTACCAG 
CCTGCAAATG ACCACTCTCC AGCTGCTGAA GGATGGATCC CTGGCAATAT CTTGGCTCCC 
CTCACTAAAC CCACTACAGA TAATAGCGAC GGAAGCATCA AGAAGTCATG TTCATGGCAT 
ACCCTGCGCA TGAGAAAGCG GGCGGAAAAG GAGAGCAGTG GCAAAAATGA AGCCAATGGG 
CCACGTAAAT CCAAGGATAT TCTGGGGAAC AAAGTCTCTG TTAAAGAGAC AAACAGCTCA 
GAGGAATCAG AGTGTGATGA CCTTGACCCC AATACTAGCA TGGAGATAAT CAACCCCAAT 
TTCATTCAGG AAGTGGCTCC AGAGTTCCTT GTGCTGTTGG TGGATATCAC CTGCCTGCTT 
GGCGACACTG TGATGCTGCA GTGCAAAGTC TGCGGCCGGC CCAAACCAAC AATAACCTGG 
AAAGGACCTG ATCAAAACAT TCTTGACAAT GACAACAGCA CGGCCACATA CACGGTGTCA 
TCCTGTGACT CTGGAGAGCT CATGCTGAAG ATCTGCAATG TGATGCCTCA GGACAGTGGC 
ATTTACACTT GTGTGGCAAC TAACGAGCAT GGAACGGCAT CAACCTCAGC CACAATCAAA 
GTGCAAGGTG TACCGGCTGC CCCAAATCGC CCCATTGCTC AGGAGAGAAG CTGCACCTCT 
GTGATCCTTC GCTGGCTGCC TCCATCCAGT ACAGGCAATT GCACCATTTC TGGCTACACA 
GTGGAGTACA GAGAAGAAGG CTCACAGGTC TGGCAGCAGT CAGTGGCCTC CACACTGGAC 
ACTTACCTTG TCATTGAAGA CCTGTCCCCA GGCTGCCAGT ATCAGTTCCG TGTGAGTGCC 
AGCAACCCCT GGGGGATCAG CTTGCCCAGT GACCCATCTG AATTCGTGAG GCTTCCAGAG 
TATGATTCTG CTGCTGATGG TGCCACTATT TCGTGGAAGG AAAACTTTGA TCTCGCTTAT 
GCGGAGCTGC ATGAGATTGG GAGGGGACGA TTCTCCATTG TAAAGAAATG CGTTCACAAA 
GCTACCCGGA AAGACGTGGC TGTAAAATTC ATCAGTAAAA AAATGAAGAA GAAGGAGCAA 
GCAGCCCACG AGGCAGCTCT GTTACAGCAC TTACAGCATC CTCAGTACAT CACTATCCAT 
GATACCTATG AGTCTCCTAC TTCTTACATC CTAGTCCTGG AGCTGATGGA TGATGGTCGT 
CTCCTAGATT ATCTAATGAA CCATGATGAA CTTATGGAGG AAAAAGTAGC TTTCTACATA 
AGAGACACAA TGGAAGCCTT GCAGTATCTT CACAATTGCC GAGTGGCTCA TTTAGATATA 
AAGCCAGAGA ATCTGCTCAT CGATTTAAGG ATCCCAGTTC CTCGTGTCAA GATCATTGAT 
TTGGAAGATG CAGTTCAAAT CACAGGCCAT TACCACGTCC ACCATCTCCT TGGAAACCCA 
GAGTTTGCAG CCCCTGAAGT TATCCAGGGC CTCCCCGTGT CCCTGAGCAC AGACATCTGG 
AGCATCGGGG TCCTCACCTA CGTCATGCTG AGCGGTGTCT CCCCGTTCCT GGATGAAAGC 
AAAGAGGAGA CGTGTATCAA TGTGTGCAGA GTCGATTTCA GCTTCCCGCC CGAGTACTTC 
TCTGATGTGA GCCATGCTGC TAGGGATTTC ATCAACGTCA TCCTCCAGGA AGACTCCAGG 
AGGAGGCCGA CAGCAGCCAC TTGTTTACAG CACCCATGGC TGCAGCCACA CAATGGCAGC 
TACTCCAAAA TCCCGCTGGA TACCTCCCGC TTGGCATCCT TCATTGAGCG CCGCAGACAC 
CAGTACGACG TGCACCCCGT CCCCGGTGTC AAGAGTTTCC TGATGAGCAG GCTGAACCCC 
GGGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145465.1
CDS	234..9122
Translation	MNPTEGAAEEGAVPDSEVDAFFRTGSFRNDGLKAADVLPILKEKVAFVSGGRDKRGGPVLTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLAKLVDPSQLTDEFEGSLDYNHDEWIELRVSLEEFFNSAIHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVIKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKELLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAVSNNKTPHNSSISHIESVLQQLDEAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDIDLATQVQELLEFLHEKQHELELNADQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIEKTHQSALQVQQKAEVLLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEIDHVIPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPGVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHNSTGESAEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKRYRDFSLRMGKYRYSLEKALGINTEDNKDLELDIIPASLSDREVKLRDANHEVNEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPAGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSSQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDSFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASSIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKQRNNSRRDGVDDADSQGDGSSQPDTISIASRTSQNTVDSDKLSGGCELTVVLQDFTAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPSGKEAYSVSSNENGGKSDSVANLQPQPSLNSIQSSPGPKRSTNTLKKWLTSPVRRLNSGKAESNIKKQKKVRDGRKSFDLGSPKTGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSSLLAARQSSSDVPTAADLVSAIEQLVKSKLTLEGGSYRGSLKDATGCLNEGMAPSTPPRNLEEEQKAKAMRGRMFVLNELVQTEKDYVKDLGTVVEGFMKRIEEKGVSEDMKGKDKIVFGNIHQIYDWHKDFFLAELEKCLQEPDRLAQLFIKHERRLHMYVVYCQNKPRSEYIVAEYEAYFEEVQQEINQRLTISDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSEIEKAVELMCLVPKRCNDMMNLGRLQGFEGKLTAQGKLLQQDTFYVTEHDSGVQSRPKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVIEENVDGDPCKFALMNRETSEKVVLQAANPEIQQAWVQDINQVLDTQRDFLNALQSPIEYQRKESSTAVLRPQAGRVAQPNSRPYSSVPVGSEKPLKATSRNPSLPPLKISTSNGSTGYEQPGDKYEQSKTDLGGCNGTSSMVVIKDYYAVKEDEICVNQGEVVQILAINQQNMFLVYQPANDHSPAAEGWIPGNILAPLTKPTTDNSDGSIKKSCSWHTLRMRKRAEKESSGKNEANGPRKSKDILGNKVSVKETNSSEESECDDLDPNTSMEIINPNFIQEVAPEFLVLLVDITCLLGDTVMLQCKVCGRPKPTITWKGPDQNILDNDNSTATYTVSSCDSGELMLKICNVMPQDSGIYTCVATNEHGTASTSATIKVQGVPAAPNRPIAQERSCTSVILRWLPPSSTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCQYQFRVSASNPWGISLPSDPSEFVRLPEYDSAADGATISWKENFDLAYAELHEIGRGRFSIVKKCVHKATRKDVAVKFISKKMKKKEQAAHEAALLQHLQHPQYITIHDTYESPTSYILVLELMDDGRLLDYLMNHDELMEEKVAFYIRDTMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKIIDLEDAVQITGHYHVHHLLGNPEFAAPEVIQGLPVSLSTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPPEYFSDVSHAARDFINVILQEDSRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLASFIERRRHQYDVHPVPGVKSFLMSRLNPGT

Target ORF information:

RefSeq Version	XM_015289979.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289979.1

ORF Insert Sequence:

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG AAGACACACC AGAGTGCCCT GCAGGTCCAG 
CAGAAAGCCG AAGTGCTGCT GCAGGCTGGG CATTATGATG CTGATGCCAT CAGAGAATGC 
GCTGAAAAGG TGGCCCTGCA CTGGCAGCAG CTGATGCTGA AGATGGAGGA CAGGCTCAAG 
CTGGTCAATG CCTCGGTGGC CTTCTACAAA ACCTCTGAGC AGGTGTGCAG TGTATTAGAG 
AGCCTGGAGC AAGAATACAG ACGTGATGAA GACTGGTGTG GAGGTCGAGA TAAACTTGGA 
CCAGCAGCTG AGATAGACCA CGTCATCCCT CTGATCAGCA AGCATCTGGA GCAGAAGGAG 
GCTTTTCTCA AGGCCTGCAC ACTGGCTCGA AGGAATGCTG AGGTATTCCT CAAGTACATC 
CACAGGAACA ATGTCAGCAT GCCCGGAGTC GCAAGCCATA CACGGGGACC TGAGCAGCAA 
GTGAAAGCCA TCCTGAGTGA GTTGCTGCAG AGGGAGAACC GGGTCCTTCA CTTCTGGACC 
CTGAAGAAGC GGAGGTTAGA TCAGTGCCAG CAGTACGTTG TATTTGAGCG CAGTGCCAAG 
CAGGCCCTGG ACTGGATCCA AGAGACAGGC GAATATTACC TCTCTACTCA CAACTCTACA 
GGGGAATCAG CAGAAGAAAC TCAGGAACTC CTGAAGGAAT ATGGGGAATT CAGAGTACCT 
GCGAAGCAAA CAAAGGAGAA AGTGAAGCTC CTCATCCAGC TGGCTGACAG CTTTGTAGAA 
AAAGGACATA TACACGCCAC TGAAATCAGG AAGTGGGTCA CCACCGTTGA CAAACGCTAC 
CGTGACTTCT CTCTCAGGAT GGGGAAATAC CGCTACTCCC TGGAGAAAGC CCTTGGAATC 
AATACAGAGG ATAACAAGGA CCTCGAACTA GATATTATTC CAGCAAGTCT TTCAGACCGG 
GAGGTAAAGC TGCGAGATGC AAATCACGAA GTCAATGAAG AAAAAAGAAA GTCTGCTAGA 
AAGAAAGAAT TCATTATGGC CGAGCTTCTT CAGACAGAGA AAGCTTATGT CAGGGACTTA 
CATGAATGTT TAGAGACTTA CCTTTGGGAA ATGACAAGTG GGGTGGAAGA AATACCCGCT 
GGGATCCTTA ATAAAGAACA CATCATCTTT GGCAATATTC AGGAGATATA TGACTTCCAT 
AACAACATTT TCCTGAAAGA ACTCGAGAAG TATGAGCAAC TGCCTGAGGA TGTGGGTCAC 
TGCTTTGTCA CCTGGGCAGA TAAATTTCAG ATGTATGTCA CCTACTGCAA AAACAAGCCT 
GACTCCAGCC AGCTCATCCT GGAACACGCA GGGACTTTCT TTGATGAAAT TCAGCAAAGG 
CATGGTCTGG CCAACTCTAT CTCTTCTTAT TTAATCAAGC CTGTCCAGAG GATCACAAAA 
TATCAACTCC TCCTGAAGGA ACTGCTTACC TGCTGTGAGG AAGGCAAAGG GGAACTCAAG 
GATGGTCTGG AAGTGATGCT CAGTGTCCCC AAGAAAGCCA ATGATGCAAT GCACGTCAGC 
ATGCTGGAAG GGTTTGATGA GAATCTAGAC GTCCAGGGAG AGCTGATTCT TCAGGATTCC 
TTCCAAGTGT GGGACCCCAA GTCTCTCATT CGGAAAGGCC GTGAGAGACA TTTGTTTCTC 
TTTGAAATCT CTTTGGTGTT TAGCAAAGAG ATCAAAGACT CTTCAGGACA CACAAAATAT 
GTTTACAAGA ACAAGTTACT GACCTCAGAA CTGGGAGTGA CTGAACATGT TGAAGGAGAT 
CCCTGTAAAT TTGCCTTGTG GTCTGGACGA ACACCATCCT CAGACAATAA AACAGTGCTG 
AAAGCATCGA GTATTGAAAC AAAACAGGAG TGGATCAAAA ATATCCGAGA AGTCATCCAA 
GAAAGAATTA TCCATCTGAA AGGAGCTCTG AAAGAGCCAA TTCAGCTCCC CAAGACACCA 
GCAAAGCAGC GAAACAACAG TAGAAGAGAT GGAGTAGATG ATGCAGACAG CCAAGGAGAT 
GGAAGCAGTC AACCTGACAC CATTTCCATT GCATCCAGGA CATCACAGAA TACAGTGGAC 
AGTGATAAGT TGTCTGGAGG GTGTGAACTA ACCGTGGTGC TCCAGGACTT CACGGCGGGC 
CACAGCAGCG AGCTGAGCAT TCAGGTGGGG CAGACAGTGG AGCTGCTGGA GAGGCCAAGT 
GAAAGGCCAG GCTGGTGCTT GGTGCGGACC ACGGAGCGGA GCCCACCTCA GGAAGGCCTG 
GTCCCCAGCA GCGCTCTCTG CATTTCCCAT TCCCGCAGCA GCGTGGAGAT GGATTGCTTC 
TTCCCTTCAG GAAAAGAGGC ATATTCAGTC TCTTCCAATG AAAACGGGGG CAAGTCCGAT 
TCAGTGGCCA ACTTACAGCC CCAGCCATCC CTCAACTCCA TCCAGAGCTC TCCCGGCCCC 
AAGCGCTCGA CCAACACGCT GAAGAAATGG CTGACGAGCC CCGTGCGCCG GCTGAACAGC 
GGGAAGGCCG AGAGCAACAT CAAGAAGCAA AAGAAGGTGC GAGATGGCAG GAAGAGCTTT 
GACCTCGGCT CGCCGAAGAC TGGAGATGAA ACCACTCCTC AAGGAGACAG CGCGGATGAG 
AAGAGCAAGA AGGGTTGGGG AGAAGATGAG CCAGATGAAG AGTCTCACAC ACCTCTTCCT 
CCTCCTATGA AGATTTTTGA CAATGATCCA ACCCAAGATG AGATGTCCTC TTCTTTGCTA 
GCTGCTCGGC AGAGCTCCTC CGATGTCCCA ACTGCTGCGG ATCTTGTCAG TGCAATAGAA 
CAGTTGGTCA AAAGTAAGCT GACCCTTGAA GGAGGATCTT ACCGAGGAAG CTTAAAAGAT 
GCCACAGGCT GCTTGAATGA AGGAATGGCG CCTTCAACTC CCCCAAGAAA CCTTGAAGAG 
GAACAAAAAG CCAAAGCAAT GAGAGGCAGG ATGTTTGTCT TAAATGAACT GGTCCAGACT 
GAAAAAGACT ACGTCAAAGA CTTGGGAACC GTTGTGGAGG GCTTCATGAA GAGAATAGAA 
GAAAAGGGAG TTTCCGAAGA TATGAAAGGG AAAGACAAGA TAGTGTTTGG CAATATTCAC 
CAGATCTATG ACTGGCACAA AGACTTTTTC CTGGCTGAAC TGGAAAAATG TCTTCAGGAG 
CCTGACCGTT TAGCACAGCT TTTTATTAAA CATGAGCGGC GATTACACAT GTATGTGGTG 
TACTGCCAAA ACAAGCCACG ATCTGAATAT ATAGTAGCTG AATACGAGGC ATATTTTGAG 
GAGGTTCAAC AGGAAATAAA CCAACGGCTG ACCATCAGTG ACTTTCTGAT CAAACCCATT 
CAGAGAATAA CTAAATATCA GCTTCTTCTC AAGGACTTCC TGAGGTACAG TGAAAAAGCT 
GGTCTGGAGT GCTCTGAAAT AGAGAAAGCA GTTGAGTTAA TGTGCCTTGT ACCAAAACGT 
TGCAATGATA TGATGAACCT GGGTCGCCTC CAAGGCTTTG AGGGTAAGCT GACAGCCCAG 
GGCAAGCTGC TGCAGCAGGA CACCTTCTAC GTGACAGAGC ATGACTCCGG AGTTCAGTCC 
CGACCAAAGG AACGTAGGGT GTTTCTCTTT GAGCAAATAG TTATCTTCAG CGAGCTGCTT 
AGGAAAGGGT CTTTAACACC AGGCTACATG TTCAAGAGAA GCATAAAGAT GAACTACCTT 
GTCATTGAAG AAAATGTGGA TGGTGATCCC TGCAAGTTTG CTCTAATGAA TCGGGAAACA 
TCTGAGAAAG TTGTTTTACA GGCAGCTAAT CCAGAAATTC AGCAGGCTTG GGTACAGGAC 
ATCAACCAGG TCCTGGACAC ACAAAGGGAT TTCCTAAATG CACTGCAGTC TCCCATAGAG 
TACCAACGCA AAGAAAGTAG CACAGCAGTG CTGAGGCCCC AGGCTGGTAG GGTCGCTCAG 
CCCAACAGCA GACCCTATTC TTCTGTTCCA GTAGGGTCTG AGAAGCCTTT GAAGGCTACA 
AGCCGTAACC CATCTCTCCC ACCCCTGAAG ATATCTACCT CCAACGGCAG TACAGGGTAT 
GAACAGCCCG GAGACAAGTA TGAACAAAGC AAGACTGATT TGGGAGGGTG CAATGGGACA 
TCTTCCATGG TGGTGATTAA AGATTACTAT GCAGTGAAGG AGGACGAGAT CTGTGTGAAT 
CAAGGAGAGG TGGTTCAGAT CCTTGCTATC AACCAGCAAA ACATGTTCCT GGTGTACCAG 
CCTGCAAATG ACCACTCTCC AGCTGCTGAA GGATGGATCC CTGGCAATAT CTTGGCTCCC 
CTCACTAAAC CCACTACAGA TAATAGCGAC GGAAGCATCA AGAAGTCATG TTCATGGCAT 
ACCCTGCGCA TGAGAAAGCG GGCGGAAAAG GAGAGCAGTG GCAAAAATGA AGCCAATGGG 
CCACGTAAAT CCAAGGATAT TCTGGGGAAC AAAGTCTCTG TTAAAGAGAC AAACAGCTCA 
GAGGAATCAG AGTGTGATGA CCTTGACCCC AATACTAGCA TGGAGATAAT CAACCCCAAT 
TTCATTCAGG AAGTGGCTCC AGAGTTCCTT GTGCTGTTGG TGGATATCAC CTGCCTGCTT 
GGCGACACTG TGATGCTGCA GTGCAAAGTC TGCGGCCGGC CCAAACCAAC AATAACCTGG 
AAAGGACCTG ATCAAAACAT TCTTGACAAT GACAACAGCA CGGCCACATA CACGGTGTCA 
TCCTGTGACT CTGGAGAGCT CATGCTGAAG ATCTGCAATG TGATGCCTCA GGACAGTGGC 
ATTTACACTT GTGTGGCAAC TAACGAGCAT GGAACGGCAT CAACCTCAGC CACAATCAAA 
GTGCAAGGTG TACCGGCTGC CCCAAATCGC CCCATTGCTC AGGAGAGAAG CTGCACCTCT 
GTGATCCTTC GCTGGCTGCC TCCATCCAGT ACAGGCAATT GCACCATTTC TGGCTACACA 
GTGGAGTACA GAGAAGAAGG CTCACAGGTC TGGCAGCAGT CAGTGGCCTC CACACTGGAC 
ACTTACCTTG TCATTGAAGA CCTGTCCCCA GGCTGCCAGT ATCAGTTCCG TGTGAGTGCC 
AGCAACCCCT GGGGGATCAG CTTGCCCAGT GACCCATCTG AATTCGTGAG GCTTCCAGAG 
TATGATTCTG CTGCTGATGG TGCCACTATT TCGTGGAAGG AAAACTTTGA TCTCGCTTAT 
GCGGAGCTGC ATGAGATTGG GAGGGGACGA TTCTCCATTG TAAAGAAATG CGTTCACAAA 
GCTACCCGGA AAGACGTGGC TGTAAAATTC ATCAGTAAAA AAATGAAGAA GAAGGAGCAA 
GCAGCCCACG AGGCAGCTCT GTTACAGCAC TTACAGCATC CTCAGTACAT CACTATCCAT 
GATACCTATG AGTCTCCTAC TTCTTACATC CTAGTCCTGG AGCTGATGGA TGATGGTCGT 
CTCCTAGATT ATCTAATGAA CCATGATGAA CTTATGGAGG AAAAAGTAGC TTTCTACATA 
AGAGACACAA TGGAAGCCTT GCAGTATCTT CACAATTGCC GAGTGGCTCA TTTAGATATA 
AAGCCAGAGA ATCTGCTCAT CGATTTAAGG ATCCCAGTTC CTCGTGTCAA GATCATTGAT 
TTGGAAGATG CAGTTCAAAT CACAGGCCAT TACCACGTCC ACCATCTCCT TGGAAACCCA 
GAGTTTGCAG CCCCTGAAGT TATCCAGGGC CTCCCCGTGT CCCTGAGCAC AGACATCTGG 
AGCATCGGGG TCCTCACCTA CGTCATGCTG AGCGGTGTCT CCCCGTTCCT GGATGAAAGC 
AAAGAGGAGA CGTGTATCAA TGTGTGCAGA GTCGATTTCA GCTTCCCGCC CGAGTACTTC 
TCTGATGTGA GCCATGCTGC TAGGGATTTC ATCAACGTCA TCCTCCAGGA AGACTCCAGG 
AGGAGGCCGA CAGCAGCCAC TTGTTTACAG CACCCATGGC TGCAGCCACA CAATGGCAGC 
TACTCCAAAA TCCCGCTGGA TACCTCCCGC TTGGCATCCT TCATTGAGCG CCGCAGACAC 
CAGTACGACG TGCACCCCGT CCCCGGTGTC AAGAGTTTCC TGATGAGCAG GCTGAACCCC 
GGGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KALRN ( XM_015289980.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145466.1 Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X5, mRNA.

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CloneID	OGa38676
Clone ID Related Accession (Same CDS sequence)	XM_015289980.2 , XM_015289980.1
Accession Version	XM_015289980.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	kalirin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289980.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGCATT TCGTCTACGG CAGAGACACA 
GGACAGTTGT CTGGAGGGTG TGAACTAACC GTGGTGCTCC AGGACTTCAC GGCGGGCCAC 
AGCAGCGAGC TGAGCATTCA GGTGGGGCAG ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA 
AGGCCAGGCT GGTGCTTGGT GCGGACCACG GAGCGGAGCC CACCTCAGGA AGGCCTGGTC 
CCCAGCAGCG CTCTCTGCAT TTCCCATTCC CGCAGCAGCG TGGAGATGGA TTGCTTCTTC 
CCTTCAGGAA AAGAGGCATA TTCAGTCTCT TCCAATGAAA ACGGGGGCAA GTCCGATTCA 
GTGGCCAACT TACAGCCCCA GCCATCCCTC AACTCCATCC AGAGCTCTCC CGGCCCCAAG 
CGCTCGACCA ACACGCTGAA GAAATGGCTG ACGAGCCCCG TGCGCCGGCT GAACAGCGGG 
AAGGCCGAGA GCAACATCAA GAAGCAAAAG AAGGTGCGAG ATGGCAGGAA GAGCTTTGAC 
CTCGGCTCGC CGAAGACTGG AGATGAAACC ACTCCTCAAG GAGACAGCGC GGATGAGAAG 
AGCAAGAAGG GTTGGGGAGA AGATGAGCCA GATGAAGAGT CTCACACACC TCTTCCTCCT 
CCTATGAAGA TTTTTGACAA TGATCCAACC CAAGATGAGA TGTCCTCTTC TTTGCTAGCT 
GCTCGGCAGA GCTCCTCCGA TGTCCCAACT GCTGCGGATC TTGTCAGTGC AATAGAACAG 
TTGGTCAAAA GTAAGCTGAC CCTTGAAGGA GGATCTTACC GAGGAAGCTT AAAAGATGCC 
ACAGGCTGCT TGAATGAAGG AATGGCGCCT TCAACTCCCC CAAGAAACCT TGAAGAGGAA 
CAAAAAGCCA AAGCAATGAG AGGCAGGATG TTTGTCTTAA ATGAACTGGT CCAGACTGAA 
AAAGACTACG TCAAAGACTT GGGAACCGTT GTGGAGGGCT TCATGAAGAG AATAGAAGAA 
AAGGGAGTTT CCGAAGATAT GAAAGGGAAA GACAAGATAG TGTTTGGCAA TATTCACCAG 
ATCTATGACT GGCACAAAGA CTTTTTCCTG GCTGAACTGG AAAAATGTCT TCAGGAGCCT 
GACCGTTTAG CACAGCTTTT TATTAAACAT GAGCGGCGAT TACACATGTA TGTGGTGTAC 
TGCCAAAACA AGCCACGATC TGAATATATA GTAGCTGAAT ACGAGGCATA TTTTGAGGAG 
GTTCAACAGG AAATAAACCA ACGGCTGACC ATCAGTGACT TTCTGATCAA ACCCATTCAG 
AGAATAACTA AATATCAGCT TCTTCTCAAG GACTTCCTGA GGTACAGTGA AAAAGCTGGT 
CTGGAGTGCT CTGAAATAGA GAAAGCAGTT GAGTTAATGT GCCTTGTACC AAAACGTTGC 
AATGATATGA TGAACCTGGG TCGCCTCCAA GGCTTTGAGG GTAAGCTGAC AGCCCAGGGC 
AAGCTGCTGC AGCAGGACAC CTTCTACGTG ACAGAGCATG ACTCCGGAGT TCAGTCCCGA 
CCAAAGGAAC GTAGGGTGTT TCTCTTTGAG CAAATAGTTA TCTTCAGCGA GCTGCTTAGG 
AAAGGGTCTT TAACACCAGG CTACATGTTC AAGAGAAGCA TAAAGATGAA CTACCTTGTC 
ATTGAAGAAA ATGTGGATGG TGATCCCTGC AAGTTTGCTC TAATGAATCG GGAAACATCT 
GAGAAAGTTG TTTTACAGGC AGCTAATCCA GAAATTCAGC AGGCTTGGGT ACAGGACATC 
AACCAGGTCC TGGACACACA AAGGGATTTC CTAAATGCAC TGCAGTCTCC CATAGAGTAC 
CAACGCAAAG AAAGTAGCAC AGCAGTGCTG AGGCCCCAGG CTGGTAGGGT CGCTCAGCCC 
AACAGCAGAC CCTATTCTTC TGTTCCAGTA GGGTCTGAGA AGCCTTTGAA GGCTACAAGC 
CGTAACCCAT CTCTCCCACC CCTGAAGATA TCTACCTCCA ACGGCAGTAC AGGGTATGAA 
CAGCCCGGAG ACAAGTATGA ACAAAGCAAG ACTGATTTGG GAGGGTGCAA TGGGACATCT 
TCCATGGTGG TGATTAAAGA TTACTATGCA GTGAAGGAGG ACGAGATCTG TGTGAATCAA 
GGAGAGGTGG TTCAGATCCT TGCTATCAAC CAGCAAAACA TGTTCCTGGT GTACCAGCCT 
GCAAATGACC ACTCTCCAGC TGCTGAAGGA TGGATCCCTG GCAATATCTT GGCTCCCCTC 
ACTAAACCCA CTACAGATAA TAGCGACGGA AGCATCAAGA AGTCATGTTC ATGGCATACC 
CTGCGCATGA GAAAGCGGGC GGAAAAGGAG AGCAGTGGCA AAAATGAAGC CAATGGGCCA 
CGTAAATCCA AGGATATTCT GGGGAACAAA GTCTCTGTTA AAATAATCAA CCCCAATTTC 
ATTCAGGAAG TGGCTCCAGA GTTCCTTGTG CTGTTGGTGG ATATCACCTG CCTGCTTGGC 
GACACTGTGA TGCTGCAGTG CAAAGTCTGC GGCCGGCCCA AACCAACAAT AACCTGGAAA 
GGACCTGATC AAAACATTCT TGACAATGAC AACAGCACGG CCACATACAC GGTGTCATCC 
TGTGACTCTG GAGAGCTCAT GCTGAAGATC TGCAATGTGA TGCCTCAGGA CAGTGGCATT 
TACACTTGTG TGGCAACTAA CGAGCATGGA ACGGCATCAA CCTCAGCCAC AATCAAAGTG 
CAAGGTGTAC CGGCTGCCCC AAATCGCCCC ATTGCTCAGG AGAGAAGCTG CACCTCTGTG 
ATCCTTCGCT GGCTGCCTCC ATCCAGTACA GGCAATTGCA CCATTTCTGG CTACACAGTG 
GAGTACAGAG AAGAAGGCTC ACAGGTCTGG CAGCAGTCAG TGGCCTCCAC ACTGGACACT 
TACCTTGTCA TTGAAGACCT GTCCCCAGGC TGCCAGTATC AGTTCCGTGT GAGTGCCAGC 
AACCCCTGGG GGATCAGCTT GCCCAGTGAC CCATCTGAAT TCGTGAGGCT TCCAGAGTAT 
GATTCTGCTG CTGATGGTGC CACTATTTCG TGGAAGGAAA ACTTTGATCT CGCTTATGCG 
GAGCTGCATG AGATTGGGAG GGGACGATTC TCCATTGTAA AGAAATGCGT TCACAAAGCT 
ACCCGGAAAG ACGTGGCTGT AAAATTCATC AGTAAAAAAA TGAAGAAGAA GGAGCAAGCA 
GCCCACGAGG CAGCTCTGTT ACAGCACTTA CAGCATCCTC AGTACATCAC TATCCATGAT 
ACCTATGAGT CTCCTACTTC TTACATCCTA GTCCTGGAGC TGATGGATGA TGGTCGTCTC 
CTAGATTATC TAATGAACCA TGATGAACTT ATGGAGGAAA AAGTAGCTTT CTACATAAGA 
GACACAATGG AAGCCTTGCA GTATCTTCAC AATTGCCGAG TGGCTCATTT AGATATAAAG 
CCAGAGAATC TGCTCATCGA TTTAAGGATC CCAGTTCCTC GTGTCAAGAT CATTGATTTG 
GAAGATGCAG TTCAAATCAC AGGCCATTAC CACGTCCACC ATCTCCTTGG AAACCCAGAG 
TTTGCAGCCC CTGAAGTTAT CCAGGGCCTC CCCGTGTCCC TGAGCACAGA CATCTGGAGC 
ATCGGGGTCC TCACCTACGT CATGCTGAGC GGTGTCTCCC CGTTCCTGGA TGAAAGCAAA 
GAGGAGACGT GTATCAATGT GTGCAGAGTC GATTTCAGCT TCCCGCCCGA GTACTTCTCT 
GATGTGAGCC ATGCTGCTAG GGATTTCATC AACGTCATCC TCCAGGAAGA CTCCAGGAGG 
AGGCCGACAG CAGCCACTTG TTTACAGCAC CCATGGCTGC AGCCACACAA TGGCAGCTAC 
TCCAAAATCC CGCTGGATAC CTCCCGCTTG GCATCCTTCA TTGAGCGCCG CAGACACCAG 
TACGACGTGC ACCCCGTCCC CGGTGTCAAG AGTTTCCTGA TGAGCAGGCT GAACCCCGGG 
ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145466.1
CDS	244..9129
Translation	MNPTEGAAEEGAVPDSEVDAFFRTGSFRNDGLKAADVLPILKEKVAFVSGGRDKRGGPVLTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLAKLVDPSQLTDEFEGSLDYNHDEWIELRVSLEEFFNSAIHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVIKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKELLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAVSNNKTPHNSSISHIESVLQQLDEAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDIDLATQVQELLEFLHEKQHELELNADQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEVLLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEIDHVIPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPGVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHNSTGESAEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKRYRDFSLRMGKYRYSLEKALGINTEDNKDLELDIIPASLSDREVKLRDANHEVNEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPAGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSSQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDSFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASSIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKQRNNSRRDGVDDADSQGDGSSQPDTISIASRTSQNTVDSDKHFVYGRDTGQLSGGCELTVVLQDFTAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPSGKEAYSVSSNENGGKSDSVANLQPQPSLNSIQSSPGPKRSTNTLKKWLTSPVRRLNSGKAESNIKKQKKVRDGRKSFDLGSPKTGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSSLLAARQSSSDVPTAADLVSAIEQLVKSKLTLEGGSYRGSLKDATGCLNEGMAPSTPPRNLEEEQKAKAMRGRMFVLNELVQTEKDYVKDLGTVVEGFMKRIEEKGVSEDMKGKDKIVFGNIHQIYDWHKDFFLAELEKCLQEPDRLAQLFIKHERRLHMYVVYCQNKPRSEYIVAEYEAYFEEVQQEINQRLTISDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSEIEKAVELMCLVPKRCNDMMNLGRLQGFEGKLTAQGKLLQQDTFYVTEHDSGVQSRPKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVIEENVDGDPCKFALMNRETSEKVVLQAANPEIQQAWVQDINQVLDTQRDFLNALQSPIEYQRKESSTAVLRPQAGRVAQPNSRPYSSVPVGSEKPLKATSRNPSLPPLKISTSNGSTGYEQPGDKYEQSKTDLGGCNGTSSMVVIKDYYAVKEDEICVNQGEVVQILAINQQNMFLVYQPANDHSPAAEGWIPGNILAPLTKPTTDNSDGSIKKSCSWHTLRMRKRAEKESSGKNEANGPRKSKDILGNKVSVKIINPNFIQEVAPEFLVLLVDITCLLGDTVMLQCKVCGRPKPTITWKGPDQNILDNDNSTATYTVSSCDSGELMLKICNVMPQDSGIYTCVATNEHGTASTSATIKVQGVPAAPNRPIAQERSCTSVILRWLPPSSTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCQYQFRVSASNPWGISLPSDPSEFVRLPEYDSAADGATISWKENFDLAYAELHEIGRGRFSIVKKCVHKATRKDVAVKFISKKMKKKEQAAHEAALLQHLQHPQYITIHDTYESPTSYILVLELMDDGRLLDYLMNHDELMEEKVAFYIRDTMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKIIDLEDAVQITGHYHVHHLLGNPEFAAPEVIQGLPVSLSTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPPEYFSDVSHAARDFINVILQEDSRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLASFIERRRHQYDVHPVPGVKSFLMSRLNPGT

Target ORF information:

RefSeq Version	XM_015289980.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289980.2

ORF Insert Sequence:

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGCATT TCGTCTACGG CAGAGACACA 
GGACAGTTGT CTGGAGGGTG TGAACTAACC GTGGTGCTCC AGGACTTCAC GGCGGGCCAC 
AGCAGCGAGC TGAGCATTCA GGTGGGGCAG ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA 
AGGCCAGGCT GGTGCTTGGT GCGGACCACG GAGCGGAGCC CACCTCAGGA AGGCCTGGTC 
CCCAGCAGCG CTCTCTGCAT TTCCCATTCC CGCAGCAGCG TGGAGATGGA TTGCTTCTTC 
CCTTCAGGAA AAGAGGCATA TTCAGTCTCT TCCAATGAAA ACGGGGGCAA GTCCGATTCA 
GTGGCCAACT TACAGCCCCA GCCATCCCTC AACTCCATCC AGAGCTCTCC CGGCCCCAAG 
CGCTCGACCA ACACGCTGAA GAAATGGCTG ACGAGCCCCG TGCGCCGGCT GAACAGCGGG 
AAGGCCGAGA GCAACATCAA GAAGCAAAAG AAGGTGCGAG ATGGCAGGAA GAGCTTTGAC 
CTCGGCTCGC CGAAGACTGG AGATGAAACC ACTCCTCAAG GAGACAGCGC GGATGAGAAG 
AGCAAGAAGG GTTGGGGAGA AGATGAGCCA GATGAAGAGT CTCACACACC TCTTCCTCCT 
CCTATGAAGA TTTTTGACAA TGATCCAACC CAAGATGAGA TGTCCTCTTC TTTGCTAGCT 
GCTCGGCAGA GCTCCTCCGA TGTCCCAACT GCTGCGGATC TTGTCAGTGC AATAGAACAG 
TTGGTCAAAA GTAAGCTGAC CCTTGAAGGA GGATCTTACC GAGGAAGCTT AAAAGATGCC 
ACAGGCTGCT TGAATGAAGG AATGGCGCCT TCAACTCCCC CAAGAAACCT TGAAGAGGAA 
CAAAAAGCCA AAGCAATGAG AGGCAGGATG TTTGTCTTAA ATGAACTGGT CCAGACTGAA 
AAAGACTACG TCAAAGACTT GGGAACCGTT GTGGAGGGCT TCATGAAGAG AATAGAAGAA 
AAGGGAGTTT CCGAAGATAT GAAAGGGAAA GACAAGATAG TGTTTGGCAA TATTCACCAG 
ATCTATGACT GGCACAAAGA CTTTTTCCTG GCTGAACTGG AAAAATGTCT TCAGGAGCCT 
GACCGTTTAG CACAGCTTTT TATTAAACAT GAGCGGCGAT TACACATGTA TGTGGTGTAC 
TGCCAAAACA AGCCACGATC TGAATATATA GTAGCTGAAT ACGAGGCATA TTTTGAGGAG 
GTTCAACAGG AAATAAACCA ACGGCTGACC ATCAGTGACT TTCTGATCAA ACCCATTCAG 
AGAATAACTA AATATCAGCT TCTTCTCAAG GACTTCCTGA GGTACAGTGA AAAAGCTGGT 
CTGGAGTGCT CTGAAATAGA GAAAGCAGTT GAGTTAATGT GCCTTGTACC AAAACGTTGC 
AATGATATGA TGAACCTGGG TCGCCTCCAA GGCTTTGAGG GTAAGCTGAC AGCCCAGGGC 
AAGCTGCTGC AGCAGGACAC CTTCTACGTG ACAGAGCATG ACTCCGGAGT TCAGTCCCGA 
CCAAAGGAAC GTAGGGTGTT TCTCTTTGAG CAAATAGTTA TCTTCAGCGA GCTGCTTAGG 
AAAGGGTCTT TAACACCAGG CTACATGTTC AAGAGAAGCA TAAAGATGAA CTACCTTGTC 
ATTGAAGAAA ATGTGGATGG TGATCCCTGC AAGTTTGCTC TAATGAATCG GGAAACATCT 
GAGAAAGTTG TTTTACAGGC AGCTAATCCA GAAATTCAGC AGGCTTGGGT ACAGGACATC 
AACCAGGTCC TGGACACACA AAGGGATTTC CTAAATGCAC TGCAGTCTCC CATAGAGTAC 
CAACGCAAAG AAAGTAGCAC AGCAGTGCTG AGGCCCCAGG CTGGTAGGGT CGCTCAGCCC 
AACAGCAGAC CCTATTCTTC TGTTCCAGTA GGGTCTGAGA AGCCTTTGAA GGCTACAAGC 
CGTAACCCAT CTCTCCCACC CCTGAAGATA TCTACCTCCA ACGGCAGTAC AGGGTATGAA 
CAGCCCGGAG ACAAGTATGA ACAAAGCAAG ACTGATTTGG GAGGGTGCAA TGGGACATCT 
TCCATGGTGG TGATTAAAGA TTACTATGCA GTGAAGGAGG ACGAGATCTG TGTGAATCAA 
GGAGAGGTGG TTCAGATCCT TGCTATCAAC CAGCAAAACA TGTTCCTGGT GTACCAGCCT 
GCAAATGACC ACTCTCCAGC TGCTGAAGGA TGGATCCCTG GCAATATCTT GGCTCCCCTC 
ACTAAACCCA CTACAGATAA TAGCGACGGA AGCATCAAGA AGTCATGTTC ATGGCATACC 
CTGCGCATGA GAAAGCGGGC GGAAAAGGAG AGCAGTGGCA AAAATGAAGC CAATGGGCCA 
CGTAAATCCA AGGATATTCT GGGGAACAAA GTCTCTGTTA AAATAATCAA CCCCAATTTC 
ATTCAGGAAG TGGCTCCAGA GTTCCTTGTG CTGTTGGTGG ATATCACCTG CCTGCTTGGC 
GACACTGTGA TGCTGCAGTG CAAAGTCTGC GGCCGGCCCA AACCAACAAT AACCTGGAAA 
GGACCTGATC AAAACATTCT TGACAATGAC AACAGCACGG CCACATACAC GGTGTCATCC 
TGTGACTCTG GAGAGCTCAT GCTGAAGATC TGCAATGTGA TGCCTCAGGA CAGTGGCATT 
TACACTTGTG TGGCAACTAA CGAGCATGGA ACGGCATCAA CCTCAGCCAC AATCAAAGTG 
CAAGGTGTAC CGGCTGCCCC AAATCGCCCC ATTGCTCAGG AGAGAAGCTG CACCTCTGTG 
ATCCTTCGCT GGCTGCCTCC ATCCAGTACA GGCAATTGCA CCATTTCTGG CTACACAGTG 
GAGTACAGAG AAGAAGGCTC ACAGGTCTGG CAGCAGTCAG TGGCCTCCAC ACTGGACACT 
TACCTTGTCA TTGAAGACCT GTCCCCAGGC TGCCAGTATC AGTTCCGTGT GAGTGCCAGC 
AACCCCTGGG GGATCAGCTT GCCCAGTGAC CCATCTGAAT TCGTGAGGCT TCCAGAGTAT 
GATTCTGCTG CTGATGGTGC CACTATTTCG TGGAAGGAAA ACTTTGATCT CGCTTATGCG 
GAGCTGCATG AGATTGGGAG GGGACGATTC TCCATTGTAA AGAAATGCGT TCACAAAGCT 
ACCCGGAAAG ACGTGGCTGT AAAATTCATC AGTAAAAAAA TGAAGAAGAA GGAGCAAGCA 
GCCCACGAGG CAGCTCTGTT ACAGCACTTA CAGCATCCTC AGTACATCAC TATCCATGAT 
ACCTATGAGT CTCCTACTTC TTACATCCTA GTCCTGGAGC TGATGGATGA TGGTCGTCTC 
CTAGATTATC TAATGAACCA TGATGAACTT ATGGAGGAAA AAGTAGCTTT CTACATAAGA 
GACACAATGG AAGCCTTGCA GTATCTTCAC AATTGCCGAG TGGCTCATTT AGATATAAAG 
CCAGAGAATC TGCTCATCGA TTTAAGGATC CCAGTTCCTC GTGTCAAGAT CATTGATTTG 
GAAGATGCAG TTCAAATCAC AGGCCATTAC CACGTCCACC ATCTCCTTGG AAACCCAGAG 
TTTGCAGCCC CTGAAGTTAT CCAGGGCCTC CCCGTGTCCC TGAGCACAGA CATCTGGAGC 
ATCGGGGTCC TCACCTACGT CATGCTGAGC GGTGTCTCCC CGTTCCTGGA TGAAAGCAAA 
GAGGAGACGT GTATCAATGT GTGCAGAGTC GATTTCAGCT TCCCGCCCGA GTACTTCTCT 
GATGTGAGCC ATGCTGCTAG GGATTTCATC AACGTCATCC TCCAGGAAGA CTCCAGGAGG 
AGGCCGACAG CAGCCACTTG TTTACAGCAC CCATGGCTGC AGCCACACAA TGGCAGCTAC 
TCCAAAATCC CGCTGGATAC CTCCCGCTTG GCATCCTTCA TTGAGCGCCG CAGACACCAG 
TACGACGTGC ACCCCGTCCC CGGTGTCAAG AGTTTCCTGA TGAGCAGGCT GAACCCCGGG 
ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KALRN ( XM_015289980.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145466.1 Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X6, mRNA.

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CloneID	OGa38676
Clone ID Related Accession (Same CDS sequence)	XM_015289980.2 , XM_015289980.1
Accession Version	XM_015289980.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	kalirin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGCATT TCGTCTACGG CAGAGACACA 
GGACAGTTGT CTGGAGGGTG TGAACTAACC GTGGTGCTCC AGGACTTCAC GGCGGGCCAC 
AGCAGCGAGC TGAGCATTCA GGTGGGGCAG ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA 
AGGCCAGGCT GGTGCTTGGT GCGGACCACG GAGCGGAGCC CACCTCAGGA AGGCCTGGTC 
CCCAGCAGCG CTCTCTGCAT TTCCCATTCC CGCAGCAGCG TGGAGATGGA TTGCTTCTTC 
CCTTCAGGAA AAGAGGCATA TTCAGTCTCT TCCAATGAAA ACGGGGGCAA GTCCGATTCA 
GTGGCCAACT TACAGCCCCA GCCATCCCTC AACTCCATCC AGAGCTCTCC CGGCCCCAAG 
CGCTCGACCA ACACGCTGAA GAAATGGCTG ACGAGCCCCG TGCGCCGGCT GAACAGCGGG 
AAGGCCGAGA GCAACATCAA GAAGCAAAAG AAGGTGCGAG ATGGCAGGAA GAGCTTTGAC 
CTCGGCTCGC CGAAGACTGG AGATGAAACC ACTCCTCAAG GAGACAGCGC GGATGAGAAG 
AGCAAGAAGG GTTGGGGAGA AGATGAGCCA GATGAAGAGT CTCACACACC TCTTCCTCCT 
CCTATGAAGA TTTTTGACAA TGATCCAACC CAAGATGAGA TGTCCTCTTC TTTGCTAGCT 
GCTCGGCAGA GCTCCTCCGA TGTCCCAACT GCTGCGGATC TTGTCAGTGC AATAGAACAG 
TTGGTCAAAA GTAAGCTGAC CCTTGAAGGA GGATCTTACC GAGGAAGCTT AAAAGATGCC 
ACAGGCTGCT TGAATGAAGG AATGGCGCCT TCAACTCCCC CAAGAAACCT TGAAGAGGAA 
CAAAAAGCCA AAGCAATGAG AGGCAGGATG TTTGTCTTAA ATGAACTGGT CCAGACTGAA 
AAAGACTACG TCAAAGACTT GGGAACCGTT GTGGAGGGCT TCATGAAGAG AATAGAAGAA 
AAGGGAGTTT CCGAAGATAT GAAAGGGAAA GACAAGATAG TGTTTGGCAA TATTCACCAG 
ATCTATGACT GGCACAAAGA CTTTTTCCTG GCTGAACTGG AAAAATGTCT TCAGGAGCCT 
GACCGTTTAG CACAGCTTTT TATTAAACAT GAGCGGCGAT TACACATGTA TGTGGTGTAC 
TGCCAAAACA AGCCACGATC TGAATATATA GTAGCTGAAT ACGAGGCATA TTTTGAGGAG 
GTTCAACAGG AAATAAACCA ACGGCTGACC ATCAGTGACT TTCTGATCAA ACCCATTCAG 
AGAATAACTA AATATCAGCT TCTTCTCAAG GACTTCCTGA GGTACAGTGA AAAAGCTGGT 
CTGGAGTGCT CTGAAATAGA GAAAGCAGTT GAGTTAATGT GCCTTGTACC AAAACGTTGC 
AATGATATGA TGAACCTGGG TCGCCTCCAA GGCTTTGAGG GTAAGCTGAC AGCCCAGGGC 
AAGCTGCTGC AGCAGGACAC CTTCTACGTG ACAGAGCATG ACTCCGGAGT TCAGTCCCGA 
CCAAAGGAAC GTAGGGTGTT TCTCTTTGAG CAAATAGTTA TCTTCAGCGA GCTGCTTAGG 
AAAGGGTCTT TAACACCAGG CTACATGTTC AAGAGAAGCA TAAAGATGAA CTACCTTGTC 
ATTGAAGAAA ATGTGGATGG TGATCCCTGC AAGTTTGCTC TAATGAATCG GGAAACATCT 
GAGAAAGTTG TTTTACAGGC AGCTAATCCA GAAATTCAGC AGGCTTGGGT ACAGGACATC 
AACCAGGTCC TGGACACACA AAGGGATTTC CTAAATGCAC TGCAGTCTCC CATAGAGTAC 
CAACGCAAAG AAAGTAGCAC AGCAGTGCTG AGGCCCCAGG CTGGTAGGGT CGCTCAGCCC 
AACAGCAGAC CCTATTCTTC TGTTCCAGTA GGGTCTGAGA AGCCTTTGAA GGCTACAAGC 
CGTAACCCAT CTCTCCCACC CCTGAAGATA TCTACCTCCA ACGGCAGTAC AGGGTATGAA 
CAGCCCGGAG ACAAGTATGA ACAAAGCAAG ACTGATTTGG GAGGGTGCAA TGGGACATCT 
TCCATGGTGG TGATTAAAGA TTACTATGCA GTGAAGGAGG ACGAGATCTG TGTGAATCAA 
GGAGAGGTGG TTCAGATCCT TGCTATCAAC CAGCAAAACA TGTTCCTGGT GTACCAGCCT 
GCAAATGACC ACTCTCCAGC TGCTGAAGGA TGGATCCCTG GCAATATCTT GGCTCCCCTC 
ACTAAACCCA CTACAGATAA TAGCGACGGA AGCATCAAGA AGTCATGTTC ATGGCATACC 
CTGCGCATGA GAAAGCGGGC GGAAAAGGAG AGCAGTGGCA AAAATGAAGC CAATGGGCCA 
CGTAAATCCA AGGATATTCT GGGGAACAAA GTCTCTGTTA AAATAATCAA CCCCAATTTC 
ATTCAGGAAG TGGCTCCAGA GTTCCTTGTG CTGTTGGTGG ATATCACCTG CCTGCTTGGC 
GACACTGTGA TGCTGCAGTG CAAAGTCTGC GGCCGGCCCA AACCAACAAT AACCTGGAAA 
GGACCTGATC AAAACATTCT TGACAATGAC AACAGCACGG CCACATACAC GGTGTCATCC 
TGTGACTCTG GAGAGCTCAT GCTGAAGATC TGCAATGTGA TGCCTCAGGA CAGTGGCATT 
TACACTTGTG TGGCAACTAA CGAGCATGGA ACGGCATCAA CCTCAGCCAC AATCAAAGTG 
CAAGGTGTAC CGGCTGCCCC AAATCGCCCC ATTGCTCAGG AGAGAAGCTG CACCTCTGTG 
ATCCTTCGCT GGCTGCCTCC ATCCAGTACA GGCAATTGCA CCATTTCTGG CTACACAGTG 
GAGTACAGAG AAGAAGGCTC ACAGGTCTGG CAGCAGTCAG TGGCCTCCAC ACTGGACACT 
TACCTTGTCA TTGAAGACCT GTCCCCAGGC TGCCAGTATC AGTTCCGTGT GAGTGCCAGC 
AACCCCTGGG GGATCAGCTT GCCCAGTGAC CCATCTGAAT TCGTGAGGCT TCCAGAGTAT 
GATTCTGCTG CTGATGGTGC CACTATTTCG TGGAAGGAAA ACTTTGATCT CGCTTATGCG 
GAGCTGCATG AGATTGGGAG GGGACGATTC TCCATTGTAA AGAAATGCGT TCACAAAGCT 
ACCCGGAAAG ACGTGGCTGT AAAATTCATC AGTAAAAAAA TGAAGAAGAA GGAGCAAGCA 
GCCCACGAGG CAGCTCTGTT ACAGCACTTA CAGCATCCTC AGTACATCAC TATCCATGAT 
ACCTATGAGT CTCCTACTTC TTACATCCTA GTCCTGGAGC TGATGGATGA TGGTCGTCTC 
CTAGATTATC TAATGAACCA TGATGAACTT ATGGAGGAAA AAGTAGCTTT CTACATAAGA 
GACACAATGG AAGCCTTGCA GTATCTTCAC AATTGCCGAG TGGCTCATTT AGATATAAAG 
CCAGAGAATC TGCTCATCGA TTTAAGGATC CCAGTTCCTC GTGTCAAGAT CATTGATTTG 
GAAGATGCAG TTCAAATCAC AGGCCATTAC CACGTCCACC ATCTCCTTGG AAACCCAGAG 
TTTGCAGCCC CTGAAGTTAT CCAGGGCCTC CCCGTGTCCC TGAGCACAGA CATCTGGAGC 
ATCGGGGTCC TCACCTACGT CATGCTGAGC GGTGTCTCCC CGTTCCTGGA TGAAAGCAAA 
GAGGAGACGT GTATCAATGT GTGCAGAGTC GATTTCAGCT TCCCGCCCGA GTACTTCTCT 
GATGTGAGCC ATGCTGCTAG GGATTTCATC AACGTCATCC TCCAGGAAGA CTCCAGGAGG 
AGGCCGACAG CAGCCACTTG TTTACAGCAC CCATGGCTGC AGCCACACAA TGGCAGCTAC 
TCCAAAATCC CGCTGGATAC CTCCCGCTTG GCATCCTTCA TTGAGCGCCG CAGACACCAG 
TACGACGTGC ACCCCGTCCC CGGTGTCAAG AGTTTCCTGA TGAGCAGGCT GAACCCCGGG 
ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145466.1
CDS	234..9119
Translation	MNPTEGAAEEGAVPDSEVDAFFRTGSFRNDGLKAADVLPILKEKVAFVSGGRDKRGGPVLTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLAKLVDPSQLTDEFEGSLDYNHDEWIELRVSLEEFFNSAIHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVIKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKELLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAVSNNKTPHNSSISHIESVLQQLDEAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDIDLATQVQELLEFLHEKQHELELNADQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEVLLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEIDHVIPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPGVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHNSTGESAEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKRYRDFSLRMGKYRYSLEKALGINTEDNKDLELDIIPASLSDREVKLRDANHEVNEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPAGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSSQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDSFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASSIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKQRNNSRRDGVDDADSQGDGSSQPDTISIASRTSQNTVDSDKHFVYGRDTGQLSGGCELTVVLQDFTAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPSGKEAYSVSSNENGGKSDSVANLQPQPSLNSIQSSPGPKRSTNTLKKWLTSPVRRLNSGKAESNIKKQKKVRDGRKSFDLGSPKTGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSSLLAARQSSSDVPTAADLVSAIEQLVKSKLTLEGGSYRGSLKDATGCLNEGMAPSTPPRNLEEEQKAKAMRGRMFVLNELVQTEKDYVKDLGTVVEGFMKRIEEKGVSEDMKGKDKIVFGNIHQIYDWHKDFFLAELEKCLQEPDRLAQLFIKHERRLHMYVVYCQNKPRSEYIVAEYEAYFEEVQQEINQRLTISDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSEIEKAVELMCLVPKRCNDMMNLGRLQGFEGKLTAQGKLLQQDTFYVTEHDSGVQSRPKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVIEENVDGDPCKFALMNRETSEKVVLQAANPEIQQAWVQDINQVLDTQRDFLNALQSPIEYQRKESSTAVLRPQAGRVAQPNSRPYSSVPVGSEKPLKATSRNPSLPPLKISTSNGSTGYEQPGDKYEQSKTDLGGCNGTSSMVVIKDYYAVKEDEICVNQGEVVQILAINQQNMFLVYQPANDHSPAAEGWIPGNILAPLTKPTTDNSDGSIKKSCSWHTLRMRKRAEKESSGKNEANGPRKSKDILGNKVSVKIINPNFIQEVAPEFLVLLVDITCLLGDTVMLQCKVCGRPKPTITWKGPDQNILDNDNSTATYTVSSCDSGELMLKICNVMPQDSGIYTCVATNEHGTASTSATIKVQGVPAAPNRPIAQERSCTSVILRWLPPSSTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCQYQFRVSASNPWGISLPSDPSEFVRLPEYDSAADGATISWKENFDLAYAELHEIGRGRFSIVKKCVHKATRKDVAVKFISKKMKKKEQAAHEAALLQHLQHPQYITIHDTYESPTSYILVLELMDDGRLLDYLMNHDELMEEKVAFYIRDTMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKIIDLEDAVQITGHYHVHHLLGNPEFAAPEVIQGLPVSLSTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPPEYFSDVSHAARDFINVILQEDSRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLASFIERRRHQYDVHPVPGVKSFLMSRLNPGT

Target ORF information:

RefSeq Version	XM_015289980.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289980.1

ORF Insert Sequence:

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGCATT TCGTCTACGG CAGAGACACA 
GGACAGTTGT CTGGAGGGTG TGAACTAACC GTGGTGCTCC AGGACTTCAC GGCGGGCCAC 
AGCAGCGAGC TGAGCATTCA GGTGGGGCAG ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA 
AGGCCAGGCT GGTGCTTGGT GCGGACCACG GAGCGGAGCC CACCTCAGGA AGGCCTGGTC 
CCCAGCAGCG CTCTCTGCAT TTCCCATTCC CGCAGCAGCG TGGAGATGGA TTGCTTCTTC 
CCTTCAGGAA AAGAGGCATA TTCAGTCTCT TCCAATGAAA ACGGGGGCAA GTCCGATTCA 
GTGGCCAACT TACAGCCCCA GCCATCCCTC AACTCCATCC AGAGCTCTCC CGGCCCCAAG 
CGCTCGACCA ACACGCTGAA GAAATGGCTG ACGAGCCCCG TGCGCCGGCT GAACAGCGGG 
AAGGCCGAGA GCAACATCAA GAAGCAAAAG AAGGTGCGAG ATGGCAGGAA GAGCTTTGAC 
CTCGGCTCGC CGAAGACTGG AGATGAAACC ACTCCTCAAG GAGACAGCGC GGATGAGAAG 
AGCAAGAAGG GTTGGGGAGA AGATGAGCCA GATGAAGAGT CTCACACACC TCTTCCTCCT 
CCTATGAAGA TTTTTGACAA TGATCCAACC CAAGATGAGA TGTCCTCTTC TTTGCTAGCT 
GCTCGGCAGA GCTCCTCCGA TGTCCCAACT GCTGCGGATC TTGTCAGTGC AATAGAACAG 
TTGGTCAAAA GTAAGCTGAC CCTTGAAGGA GGATCTTACC GAGGAAGCTT AAAAGATGCC 
ACAGGCTGCT TGAATGAAGG AATGGCGCCT TCAACTCCCC CAAGAAACCT TGAAGAGGAA 
CAAAAAGCCA AAGCAATGAG AGGCAGGATG TTTGTCTTAA ATGAACTGGT CCAGACTGAA 
AAAGACTACG TCAAAGACTT GGGAACCGTT GTGGAGGGCT TCATGAAGAG AATAGAAGAA 
AAGGGAGTTT CCGAAGATAT GAAAGGGAAA GACAAGATAG TGTTTGGCAA TATTCACCAG 
ATCTATGACT GGCACAAAGA CTTTTTCCTG GCTGAACTGG AAAAATGTCT TCAGGAGCCT 
GACCGTTTAG CACAGCTTTT TATTAAACAT GAGCGGCGAT TACACATGTA TGTGGTGTAC 
TGCCAAAACA AGCCACGATC TGAATATATA GTAGCTGAAT ACGAGGCATA TTTTGAGGAG 
GTTCAACAGG AAATAAACCA ACGGCTGACC ATCAGTGACT TTCTGATCAA ACCCATTCAG 
AGAATAACTA AATATCAGCT TCTTCTCAAG GACTTCCTGA GGTACAGTGA AAAAGCTGGT 
CTGGAGTGCT CTGAAATAGA GAAAGCAGTT GAGTTAATGT GCCTTGTACC AAAACGTTGC 
AATGATATGA TGAACCTGGG TCGCCTCCAA GGCTTTGAGG GTAAGCTGAC AGCCCAGGGC 
AAGCTGCTGC AGCAGGACAC CTTCTACGTG ACAGAGCATG ACTCCGGAGT TCAGTCCCGA 
CCAAAGGAAC GTAGGGTGTT TCTCTTTGAG CAAATAGTTA TCTTCAGCGA GCTGCTTAGG 
AAAGGGTCTT TAACACCAGG CTACATGTTC AAGAGAAGCA TAAAGATGAA CTACCTTGTC 
ATTGAAGAAA ATGTGGATGG TGATCCCTGC AAGTTTGCTC TAATGAATCG GGAAACATCT 
GAGAAAGTTG TTTTACAGGC AGCTAATCCA GAAATTCAGC AGGCTTGGGT ACAGGACATC 
AACCAGGTCC TGGACACACA AAGGGATTTC CTAAATGCAC TGCAGTCTCC CATAGAGTAC 
CAACGCAAAG AAAGTAGCAC AGCAGTGCTG AGGCCCCAGG CTGGTAGGGT CGCTCAGCCC 
AACAGCAGAC CCTATTCTTC TGTTCCAGTA GGGTCTGAGA AGCCTTTGAA GGCTACAAGC 
CGTAACCCAT CTCTCCCACC CCTGAAGATA TCTACCTCCA ACGGCAGTAC AGGGTATGAA 
CAGCCCGGAG ACAAGTATGA ACAAAGCAAG ACTGATTTGG GAGGGTGCAA TGGGACATCT 
TCCATGGTGG TGATTAAAGA TTACTATGCA GTGAAGGAGG ACGAGATCTG TGTGAATCAA 
GGAGAGGTGG TTCAGATCCT TGCTATCAAC CAGCAAAACA TGTTCCTGGT GTACCAGCCT 
GCAAATGACC ACTCTCCAGC TGCTGAAGGA TGGATCCCTG GCAATATCTT GGCTCCCCTC 
ACTAAACCCA CTACAGATAA TAGCGACGGA AGCATCAAGA AGTCATGTTC ATGGCATACC 
CTGCGCATGA GAAAGCGGGC GGAAAAGGAG AGCAGTGGCA AAAATGAAGC CAATGGGCCA 
CGTAAATCCA AGGATATTCT GGGGAACAAA GTCTCTGTTA AAATAATCAA CCCCAATTTC 
ATTCAGGAAG TGGCTCCAGA GTTCCTTGTG CTGTTGGTGG ATATCACCTG CCTGCTTGGC 
GACACTGTGA TGCTGCAGTG CAAAGTCTGC GGCCGGCCCA AACCAACAAT AACCTGGAAA 
GGACCTGATC AAAACATTCT TGACAATGAC AACAGCACGG CCACATACAC GGTGTCATCC 
TGTGACTCTG GAGAGCTCAT GCTGAAGATC TGCAATGTGA TGCCTCAGGA CAGTGGCATT 
TACACTTGTG TGGCAACTAA CGAGCATGGA ACGGCATCAA CCTCAGCCAC AATCAAAGTG 
CAAGGTGTAC CGGCTGCCCC AAATCGCCCC ATTGCTCAGG AGAGAAGCTG CACCTCTGTG 
ATCCTTCGCT GGCTGCCTCC ATCCAGTACA GGCAATTGCA CCATTTCTGG CTACACAGTG 
GAGTACAGAG AAGAAGGCTC ACAGGTCTGG CAGCAGTCAG TGGCCTCCAC ACTGGACACT 
TACCTTGTCA TTGAAGACCT GTCCCCAGGC TGCCAGTATC AGTTCCGTGT GAGTGCCAGC 
AACCCCTGGG GGATCAGCTT GCCCAGTGAC CCATCTGAAT TCGTGAGGCT TCCAGAGTAT 
GATTCTGCTG CTGATGGTGC CACTATTTCG TGGAAGGAAA ACTTTGATCT CGCTTATGCG 
GAGCTGCATG AGATTGGGAG GGGACGATTC TCCATTGTAA AGAAATGCGT TCACAAAGCT 
ACCCGGAAAG ACGTGGCTGT AAAATTCATC AGTAAAAAAA TGAAGAAGAA GGAGCAAGCA 
GCCCACGAGG CAGCTCTGTT ACAGCACTTA CAGCATCCTC AGTACATCAC TATCCATGAT 
ACCTATGAGT CTCCTACTTC TTACATCCTA GTCCTGGAGC TGATGGATGA TGGTCGTCTC 
CTAGATTATC TAATGAACCA TGATGAACTT ATGGAGGAAA AAGTAGCTTT CTACATAAGA 
GACACAATGG AAGCCTTGCA GTATCTTCAC AATTGCCGAG TGGCTCATTT AGATATAAAG 
CCAGAGAATC TGCTCATCGA TTTAAGGATC CCAGTTCCTC GTGTCAAGAT CATTGATTTG 
GAAGATGCAG TTCAAATCAC AGGCCATTAC CACGTCCACC ATCTCCTTGG AAACCCAGAG 
TTTGCAGCCC CTGAAGTTAT CCAGGGCCTC CCCGTGTCCC TGAGCACAGA CATCTGGAGC 
ATCGGGGTCC TCACCTACGT CATGCTGAGC GGTGTCTCCC CGTTCCTGGA TGAAAGCAAA 
GAGGAGACGT GTATCAATGT GTGCAGAGTC GATTTCAGCT TCCCGCCCGA GTACTTCTCT 
GATGTGAGCC ATGCTGCTAG GGATTTCATC AACGTCATCC TCCAGGAAGA CTCCAGGAGG 
AGGCCGACAG CAGCCACTTG TTTACAGCAC CCATGGCTGC AGCCACACAA TGGCAGCTAC 
TCCAAAATCC CGCTGGATAC CTCCCGCTTG GCATCCTTCA TTGAGCGCCG CAGACACCAG 
TACGACGTGC ACCCCGTCCC CGGTGTCAAG AGTTTCCTGA TGAGCAGGCT GAACCCCGGG 
ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KALRN ( XM_015289981.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145467.1 Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X6, mRNA.

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CloneID	OGa38677
Clone ID Related Accession (Same CDS sequence)	XM_015289981.2 , XM_015289981.1
Accession Version	XM_015289981.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	kalirin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289981.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGCATT TCGTCTACGG CAGAGACACA 
GGACAGTTGT CTGGAGGGTG TGAACTAACC GTGGTGCTCC AGGACTTCAC GGCGGGCCAC 
AGCAGCGAGC TGAGCATTCA GGTGGGGCAG ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA 
AGGCCAGGCT GGTGCTTGGT GCGGACCACG GAGCGGAGCC CACCTCAGGA AGGCCTGGTC 
CCCAGCAGCG CTCTCTGCAT TTCCCATTCC CGCAGCAGCG TGGAGATGGA TTGCTTCTTC 
CCTTCAGGAA AAGAGGCATA TTCAGTCTCT TCCAATGAAA ACGGGGGCAA GTCCGATTCA 
GTGGCCAACT TACAGCCCCA GCCATCCCTC AACTCCATCC AGAGCTCTCC CGGCCCCAAG 
CGCTCGACCA ACACGCTGAA GAAATGGCTG ACGAGCCCCG TGCGCCGGCT GAACAGCGGG 
AAGGCCGAGA GCAACATCAA GAAGCAAAAG AAGGTGCGAG ATGGCAGGAA GAGCTTTGAC 
CTCGGCTCGC CGAAGACTGG AGATGAAACC ACTCCTCAAG GAGACAGCGC GGATGAGAAG 
AGCAAGAAGG GTTGGGGAGA AGATGAGCCA GATGAAGAGT CTCACACACC TCTTCCTCCT 
CCTATGAAGA TTTTTGACAA TGATCCAACC CAAGATGAGA TGACCCTTGA AGGAGGATCT 
TACCGAGGAA GCTTAAAAGA TGCCACAGGC TGCTTGAATG AAGGAATGGC GCCTTCAACT 
CCCCCAAGAA ACCTTGAAGA GGAACAAAAA GCCAAAGCAA TGAGAGGCAG GATGTTTGTC 
TTAAATGAAC TGGTCCAGAC TGAAAAAGAC TACGTCAAAG ACTTGGGAAC CGTTGTGGAG 
GGCTTCATGA AGAGAATAGA AGAAAAGGGA GTTTCCGAAG ATATGAAAGG GAAAGACAAG 
ATAGTGTTTG GCAATATTCA CCAGATCTAT GACTGGCACA AAGACTTTTT CCTGGCTGAA 
CTGGAAAAAT GTCTTCAGGA GCCTGACCGT TTAGCACAGC TTTTTATTAA ACATGAGCGG 
CGATTACACA TGTATGTGGT GTACTGCCAA AACAAGCCAC GATCTGAATA TATAGTAGCT 
GAATACGAGG CATATTTTGA GGAGGTTCAA CAGGAAATAA ACCAACGGCT GACCATCAGT 
GACTTTCTGA TCAAACCCAT TCAGAGAATA ACTAAATATC AGCTTCTTCT CAAGGACTTC 
CTGAGGTACA GTGAAAAAGC TGGTCTGGAG TGCTCTGAAA TAGAGAAAGC AGTTGAGTTA 
ATGTGCCTTG TACCAAAACG TTGCAATGAT ATGATGAACC TGGGTCGCCT CCAAGGCTTT 
GAGGGTAAGC TGACAGCCCA GGGCAAGCTG CTGCAGCAGG ACACCTTCTA CGTGACAGAG 
CATGACTCCG GAGTTCAGTC CCGACCAAAG GAACGTAGGG TGTTTCTCTT TGAGCAAATA 
GTTATCTTCA GCGAGCTGCT TAGGAAAGGG TCTTTAACAC CAGGCTACAT GTTCAAGAGA 
AGCATAAAGA TGAACTACCT TGTCATTGAA GAAAATGTGG ATGGTGATCC CTGCAAGTTT 
GCTCTAATGA ATCGGGAAAC ATCTGAGAAA GTTGTTTTAC AGGCAGCTAA TCCAGAAATT 
CAGCAGGCTT GGGTACAGGA CATCAACCAG GTCCTGGACA CACAAAGGGA TTTCCTAAAT 
GCACTGCAGT CTCCCATAGA GTACCAACGC AAAGAAAGTA GCACAGCAGT GCTGAGGCCC 
CAGGCTGGTA GGGTCGCTCA GCCCAACAGC AGACCCTATT CTTCTGTTCC AGTAGGGTCT 
GAGAAGCCTT TGAAGGCTAC AAGCCGTAAC CCATCTCTCC CACCCCTGAA GATATCTACC 
TCCAACGGCA GTACAGGGTA TGAACAGCCC GGAGACAAGT ATGAACAAAG CAAGACTGAT 
TTGGGAGGGT GCAATGGGAC ATCTTCCATG GTGGTGATTA AAGATTACTA TGCAGTGAAG 
GAGGACGAGA TCTGTGTGAA TCAAGGAGAG GTGGTTCAGA TCCTTGCTAT CAACCAGCAA 
AACATGTTCC TGGTGTACCA GCCTGCAAAT GACCACTCTC CAGCTGCTGA AGGATGGATC 
CCTGGCAATA TCTTGGCTCC CCTCACTAAA CCCACTACAG ATAATAGCGA CGGAAGCATC 
AAGAAGTCAT GTTCATGGCA TACCCTGCGC ATGAGAAAGC GGGCGGAAAA GGAGAGCAGT 
GGCAAAAATG AAGCCAATGG GCCACGTAAA TCCAAGGATA TTCTGGGGAA CAAAGTCTCT 
GTTAAAGAGA CAAACAGCTC AGAGGAATCA GAGTGTGATG ACCTTGACCC CAATACTAGC 
ATGGAGATAA TCAACCCCAA TTTCATTCAG GAAGTGGCTC CAGAGTTCCT TGTGCTGTTG 
GTGGATATCA CCTGCCTGCT TGGCGACACT GTGATGCTGC AGTGCAAAGT CTGCGGCCGG 
CCCAAACCAA CAATAACCTG GAAAGGACCT GATCAAAACA TTCTTGACAA TGACAACAGC 
ACGGCCACAT ACACGGTGTC ATCCTGTGAC TCTGGAGAGC TCATGCTGAA GATCTGCAAT 
GTGATGCCTC AGGACAGTGG CATTTACACT TGTGTGGCAA CTAACGAGCA TGGAACGGCA 
TCAACCTCAG CCACAATCAA AGTGCAAGGT GTACCGGCTG CCCCAAATCG CCCCATTGCT 
CAGGAGAGAA GCTGCACCTC TGTGATCCTT CGCTGGCTGC CTCCATCCAG TACAGGCAAT 
TGCACCATTT CTGGCTACAC AGTGGAGTAC AGAGAAGAAG GCTCACAGGT CTGGCAGCAG 
TCAGTGGCCT CCACACTGGA CACTTACCTT GTCATTGAAG ACCTGTCCCC AGGCTGCCAG 
TATCAGTTCC GTGTGAGTGC CAGCAACCCC TGGGGGATCA GCTTGCCCAG TGACCCATCT 
GAATTCGTGA GGCTTCCAGA GTATGATTCT GCTGCTGATG GTGCCACTAT TTCGTGGAAG 
GAAAACTTTG ATCTCGCTTA TGCGGAGCTG CATGAGATTG GGAGGGGACG ATTCTCCATT 
GTAAAGAAAT GCGTTCACAA AGCTACCCGG AAAGACGTGG CTGTAAAATT CATCAGTAAA 
AAAATGAAGA AGAAGGAGCA AGCAGCCCAC GAGGCAGCTC TGTTACAGCA CTTACAGCAT 
CCTCAGTACA TCACTATCCA TGATACCTAT GAGTCTCCTA CTTCTTACAT CCTAGTCCTG 
GAGCTGATGG ATGATGGTCG TCTCCTAGAT TATCTAATGA ACCATGATGA ACTTATGGAG 
GAAAAAGTAG CTTTCTACAT AAGAGACACA ATGGAAGCCT TGCAGTATCT TCACAATTGC 
CGAGTGGCTC ATTTAGATAT AAAGCCAGAG AATCTGCTCA TCGATTTAAG GATCCCAGTT 
CCTCGTGTCA AGATCATTGA TTTGGAAGAT GCAGTTCAAA TCACAGGCCA TTACCACGTC 
CACCATCTCC TTGGAAACCC AGAGTTTGCA GCCCCTGAAG TTATCCAGGG CCTCCCCGTG 
TCCCTGAGCA CAGACATCTG GAGCATCGGG GTCCTCACCT ACGTCATGCT GAGCGGTGTC 
TCCCCGTTCC TGGATGAAAG CAAAGAGGAG ACGTGTATCA ATGTGTGCAG AGTCGATTTC 
AGCTTCCCGC CCGAGTACTT CTCTGATGTG AGCCATGCTG CTAGGGATTT CATCAACGTC 
ATCCTCCAGG AAGACTCCAG GAGGAGGCCG ACAGCAGCCA CTTGTTTACA GCACCCATGG 
CTGCAGCCAC ACAATGGCAG CTACTCCAAA ATCCCGCTGG ATACCTCCCG CTTGGCATCC 
TTCATTGAGC GCCGCAGACA CCAGTACGAC GTGCACCCCG TCCCCGGTGT CAAGAGTTTC 
CTGATGAGCA GGCTGAACCC CGGGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145467.1
CDS	244..9093
Translation	MNPTEGAAEEGAVPDSEVDAFFRTGSFRNDGLKAADVLPILKEKVAFVSGGRDKRGGPVLTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLAKLVDPSQLTDEFEGSLDYNHDEWIELRVSLEEFFNSAIHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVIKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKELLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAVSNNKTPHNSSISHIESVLQQLDEAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDIDLATQVQELLEFLHEKQHELELNADQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEVLLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEIDHVIPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPGVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHNSTGESAEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKRYRDFSLRMGKYRYSLEKALGINTEDNKDLELDIIPASLSDREVKLRDANHEVNEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPAGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSSQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDSFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASSIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKQRNNSRRDGVDDADSQGDGSSQPDTISIASRTSQNTVDSDKHFVYGRDTGQLSGGCELTVVLQDFTAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPSGKEAYSVSSNENGGKSDSVANLQPQPSLNSIQSSPGPKRSTNTLKKWLTSPVRRLNSGKAESNIKKQKKVRDGRKSFDLGSPKTGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMTLEGGSYRGSLKDATGCLNEGMAPSTPPRNLEEEQKAKAMRGRMFVLNELVQTEKDYVKDLGTVVEGFMKRIEEKGVSEDMKGKDKIVFGNIHQIYDWHKDFFLAELEKCLQEPDRLAQLFIKHERRLHMYVVYCQNKPRSEYIVAEYEAYFEEVQQEINQRLTISDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSEIEKAVELMCLVPKRCNDMMNLGRLQGFEGKLTAQGKLLQQDTFYVTEHDSGVQSRPKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVIEENVDGDPCKFALMNRETSEKVVLQAANPEIQQAWVQDINQVLDTQRDFLNALQSPIEYQRKESSTAVLRPQAGRVAQPNSRPYSSVPVGSEKPLKATSRNPSLPPLKISTSNGSTGYEQPGDKYEQSKTDLGGCNGTSSMVVIKDYYAVKEDEICVNQGEVVQILAINQQNMFLVYQPANDHSPAAEGWIPGNILAPLTKPTTDNSDGSIKKSCSWHTLRMRKRAEKESSGKNEANGPRKSKDILGNKVSVKETNSSEESECDDLDPNTSMEIINPNFIQEVAPEFLVLLVDITCLLGDTVMLQCKVCGRPKPTITWKGPDQNILDNDNSTATYTVSSCDSGELMLKICNVMPQDSGIYTCVATNEHGTASTSATIKVQGVPAAPNRPIAQERSCTSVILRWLPPSSTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCQYQFRVSASNPWGISLPSDPSEFVRLPEYDSAADGATISWKENFDLAYAELHEIGRGRFSIVKKCVHKATRKDVAVKFISKKMKKKEQAAHEAALLQHLQHPQYITIHDTYESPTSYILVLELMDDGRLLDYLMNHDELMEEKVAFYIRDTMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKIIDLEDAVQITGHYHVHHLLGNPEFAAPEVIQGLPVSLSTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPPEYFSDVSHAARDFINVILQEDSRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLASFIERRRHQYDVHPVPGVKSFLMSRLNPGT

Target ORF information:

RefSeq Version	XM_015289981.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289981.2

ORF Insert Sequence:

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGCATT TCGTCTACGG CAGAGACACA 
GGACAGTTGT CTGGAGGGTG TGAACTAACC GTGGTGCTCC AGGACTTCAC GGCGGGCCAC 
AGCAGCGAGC TGAGCATTCA GGTGGGGCAG ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA 
AGGCCAGGCT GGTGCTTGGT GCGGACCACG GAGCGGAGCC CACCTCAGGA AGGCCTGGTC 
CCCAGCAGCG CTCTCTGCAT TTCCCATTCC CGCAGCAGCG TGGAGATGGA TTGCTTCTTC 
CCTTCAGGAA AAGAGGCATA TTCAGTCTCT TCCAATGAAA ACGGGGGCAA GTCCGATTCA 
GTGGCCAACT TACAGCCCCA GCCATCCCTC AACTCCATCC AGAGCTCTCC CGGCCCCAAG 
CGCTCGACCA ACACGCTGAA GAAATGGCTG ACGAGCCCCG TGCGCCGGCT GAACAGCGGG 
AAGGCCGAGA GCAACATCAA GAAGCAAAAG AAGGTGCGAG ATGGCAGGAA GAGCTTTGAC 
CTCGGCTCGC CGAAGACTGG AGATGAAACC ACTCCTCAAG GAGACAGCGC GGATGAGAAG 
AGCAAGAAGG GTTGGGGAGA AGATGAGCCA GATGAAGAGT CTCACACACC TCTTCCTCCT 
CCTATGAAGA TTTTTGACAA TGATCCAACC CAAGATGAGA TGACCCTTGA AGGAGGATCT 
TACCGAGGAA GCTTAAAAGA TGCCACAGGC TGCTTGAATG AAGGAATGGC GCCTTCAACT 
CCCCCAAGAA ACCTTGAAGA GGAACAAAAA GCCAAAGCAA TGAGAGGCAG GATGTTTGTC 
TTAAATGAAC TGGTCCAGAC TGAAAAAGAC TACGTCAAAG ACTTGGGAAC CGTTGTGGAG 
GGCTTCATGA AGAGAATAGA AGAAAAGGGA GTTTCCGAAG ATATGAAAGG GAAAGACAAG 
ATAGTGTTTG GCAATATTCA CCAGATCTAT GACTGGCACA AAGACTTTTT CCTGGCTGAA 
CTGGAAAAAT GTCTTCAGGA GCCTGACCGT TTAGCACAGC TTTTTATTAA ACATGAGCGG 
CGATTACACA TGTATGTGGT GTACTGCCAA AACAAGCCAC GATCTGAATA TATAGTAGCT 
GAATACGAGG CATATTTTGA GGAGGTTCAA CAGGAAATAA ACCAACGGCT GACCATCAGT 
GACTTTCTGA TCAAACCCAT TCAGAGAATA ACTAAATATC AGCTTCTTCT CAAGGACTTC 
CTGAGGTACA GTGAAAAAGC TGGTCTGGAG TGCTCTGAAA TAGAGAAAGC AGTTGAGTTA 
ATGTGCCTTG TACCAAAACG TTGCAATGAT ATGATGAACC TGGGTCGCCT CCAAGGCTTT 
GAGGGTAAGC TGACAGCCCA GGGCAAGCTG CTGCAGCAGG ACACCTTCTA CGTGACAGAG 
CATGACTCCG GAGTTCAGTC CCGACCAAAG GAACGTAGGG TGTTTCTCTT TGAGCAAATA 
GTTATCTTCA GCGAGCTGCT TAGGAAAGGG TCTTTAACAC CAGGCTACAT GTTCAAGAGA 
AGCATAAAGA TGAACTACCT TGTCATTGAA GAAAATGTGG ATGGTGATCC CTGCAAGTTT 
GCTCTAATGA ATCGGGAAAC ATCTGAGAAA GTTGTTTTAC AGGCAGCTAA TCCAGAAATT 
CAGCAGGCTT GGGTACAGGA CATCAACCAG GTCCTGGACA CACAAAGGGA TTTCCTAAAT 
GCACTGCAGT CTCCCATAGA GTACCAACGC AAAGAAAGTA GCACAGCAGT GCTGAGGCCC 
CAGGCTGGTA GGGTCGCTCA GCCCAACAGC AGACCCTATT CTTCTGTTCC AGTAGGGTCT 
GAGAAGCCTT TGAAGGCTAC AAGCCGTAAC CCATCTCTCC CACCCCTGAA GATATCTACC 
TCCAACGGCA GTACAGGGTA TGAACAGCCC GGAGACAAGT ATGAACAAAG CAAGACTGAT 
TTGGGAGGGT GCAATGGGAC ATCTTCCATG GTGGTGATTA AAGATTACTA TGCAGTGAAG 
GAGGACGAGA TCTGTGTGAA TCAAGGAGAG GTGGTTCAGA TCCTTGCTAT CAACCAGCAA 
AACATGTTCC TGGTGTACCA GCCTGCAAAT GACCACTCTC CAGCTGCTGA AGGATGGATC 
CCTGGCAATA TCTTGGCTCC CCTCACTAAA CCCACTACAG ATAATAGCGA CGGAAGCATC 
AAGAAGTCAT GTTCATGGCA TACCCTGCGC ATGAGAAAGC GGGCGGAAAA GGAGAGCAGT 
GGCAAAAATG AAGCCAATGG GCCACGTAAA TCCAAGGATA TTCTGGGGAA CAAAGTCTCT 
GTTAAAGAGA CAAACAGCTC AGAGGAATCA GAGTGTGATG ACCTTGACCC CAATACTAGC 
ATGGAGATAA TCAACCCCAA TTTCATTCAG GAAGTGGCTC CAGAGTTCCT TGTGCTGTTG 
GTGGATATCA CCTGCCTGCT TGGCGACACT GTGATGCTGC AGTGCAAAGT CTGCGGCCGG 
CCCAAACCAA CAATAACCTG GAAAGGACCT GATCAAAACA TTCTTGACAA TGACAACAGC 
ACGGCCACAT ACACGGTGTC ATCCTGTGAC TCTGGAGAGC TCATGCTGAA GATCTGCAAT 
GTGATGCCTC AGGACAGTGG CATTTACACT TGTGTGGCAA CTAACGAGCA TGGAACGGCA 
TCAACCTCAG CCACAATCAA AGTGCAAGGT GTACCGGCTG CCCCAAATCG CCCCATTGCT 
CAGGAGAGAA GCTGCACCTC TGTGATCCTT CGCTGGCTGC CTCCATCCAG TACAGGCAAT 
TGCACCATTT CTGGCTACAC AGTGGAGTAC AGAGAAGAAG GCTCACAGGT CTGGCAGCAG 
TCAGTGGCCT CCACACTGGA CACTTACCTT GTCATTGAAG ACCTGTCCCC AGGCTGCCAG 
TATCAGTTCC GTGTGAGTGC CAGCAACCCC TGGGGGATCA GCTTGCCCAG TGACCCATCT 
GAATTCGTGA GGCTTCCAGA GTATGATTCT GCTGCTGATG GTGCCACTAT TTCGTGGAAG 
GAAAACTTTG ATCTCGCTTA TGCGGAGCTG CATGAGATTG GGAGGGGACG ATTCTCCATT 
GTAAAGAAAT GCGTTCACAA AGCTACCCGG AAAGACGTGG CTGTAAAATT CATCAGTAAA 
AAAATGAAGA AGAAGGAGCA AGCAGCCCAC GAGGCAGCTC TGTTACAGCA CTTACAGCAT 
CCTCAGTACA TCACTATCCA TGATACCTAT GAGTCTCCTA CTTCTTACAT CCTAGTCCTG 
GAGCTGATGG ATGATGGTCG TCTCCTAGAT TATCTAATGA ACCATGATGA ACTTATGGAG 
GAAAAAGTAG CTTTCTACAT AAGAGACACA ATGGAAGCCT TGCAGTATCT TCACAATTGC 
CGAGTGGCTC ATTTAGATAT AAAGCCAGAG AATCTGCTCA TCGATTTAAG GATCCCAGTT 
CCTCGTGTCA AGATCATTGA TTTGGAAGAT GCAGTTCAAA TCACAGGCCA TTACCACGTC 
CACCATCTCC TTGGAAACCC AGAGTTTGCA GCCCCTGAAG TTATCCAGGG CCTCCCCGTG 
TCCCTGAGCA CAGACATCTG GAGCATCGGG GTCCTCACCT ACGTCATGCT GAGCGGTGTC 
TCCCCGTTCC TGGATGAAAG CAAAGAGGAG ACGTGTATCA ATGTGTGCAG AGTCGATTTC 
AGCTTCCCGC CCGAGTACTT CTCTGATGTG AGCCATGCTG CTAGGGATTT CATCAACGTC 
ATCCTCCAGG AAGACTCCAG GAGGAGGCCG ACAGCAGCCA CTTGTTTACA GCACCCATGG 
CTGCAGCCAC ACAATGGCAG CTACTCCAAA ATCCCGCTGG ATACCTCCCG CTTGGCATCC 
TTCATTGAGC GCCGCAGACA CCAGTACGAC GTGCACCCCG TCCCCGGTGT CAAGAGTTTC 
CTGATGAGCA GGCTGAACCC CGGGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KALRN ( XM_015289981.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145467.1 Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X7, mRNA.

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CloneID	OGa38677
Clone ID Related Accession (Same CDS sequence)	XM_015289981.2 , XM_015289981.1
Accession Version	XM_015289981.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	kalirin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGCATT TCGTCTACGG CAGAGACACA 
GGACAGTTGT CTGGAGGGTG TGAACTAACC GTGGTGCTCC AGGACTTCAC GGCGGGCCAC 
AGCAGCGAGC TGAGCATTCA GGTGGGGCAG ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA 
AGGCCAGGCT GGTGCTTGGT GCGGACCACG GAGCGGAGCC CACCTCAGGA AGGCCTGGTC 
CCCAGCAGCG CTCTCTGCAT TTCCCATTCC CGCAGCAGCG TGGAGATGGA TTGCTTCTTC 
CCTTCAGGAA AAGAGGCATA TTCAGTCTCT TCCAATGAAA ACGGGGGCAA GTCCGATTCA 
GTGGCCAACT TACAGCCCCA GCCATCCCTC AACTCCATCC AGAGCTCTCC CGGCCCCAAG 
CGCTCGACCA ACACGCTGAA GAAATGGCTG ACGAGCCCCG TGCGCCGGCT GAACAGCGGG 
AAGGCCGAGA GCAACATCAA GAAGCAAAAG AAGGTGCGAG ATGGCAGGAA GAGCTTTGAC 
CTCGGCTCGC CGAAGACTGG AGATGAAACC ACTCCTCAAG GAGACAGCGC GGATGAGAAG 
AGCAAGAAGG GTTGGGGAGA AGATGAGCCA GATGAAGAGT CTCACACACC TCTTCCTCCT 
CCTATGAAGA TTTTTGACAA TGATCCAACC CAAGATGAGA TGACCCTTGA AGGAGGATCT 
TACCGAGGAA GCTTAAAAGA TGCCACAGGC TGCTTGAATG AAGGAATGGC GCCTTCAACT 
CCCCCAAGAA ACCTTGAAGA GGAACAAAAA GCCAAAGCAA TGAGAGGCAG GATGTTTGTC 
TTAAATGAAC TGGTCCAGAC TGAAAAAGAC TACGTCAAAG ACTTGGGAAC CGTTGTGGAG 
GGCTTCATGA AGAGAATAGA AGAAAAGGGA GTTTCCGAAG ATATGAAAGG GAAAGACAAG 
ATAGTGTTTG GCAATATTCA CCAGATCTAT GACTGGCACA AAGACTTTTT CCTGGCTGAA 
CTGGAAAAAT GTCTTCAGGA GCCTGACCGT TTAGCACAGC TTTTTATTAA ACATGAGCGG 
CGATTACACA TGTATGTGGT GTACTGCCAA AACAAGCCAC GATCTGAATA TATAGTAGCT 
GAATACGAGG CATATTTTGA GGAGGTTCAA CAGGAAATAA ACCAACGGCT GACCATCAGT 
GACTTTCTGA TCAAACCCAT TCAGAGAATA ACTAAATATC AGCTTCTTCT CAAGGACTTC 
CTGAGGTACA GTGAAAAAGC TGGTCTGGAG TGCTCTGAAA TAGAGAAAGC AGTTGAGTTA 
ATGTGCCTTG TACCAAAACG TTGCAATGAT ATGATGAACC TGGGTCGCCT CCAAGGCTTT 
GAGGGTAAGC TGACAGCCCA GGGCAAGCTG CTGCAGCAGG ACACCTTCTA CGTGACAGAG 
CATGACTCCG GAGTTCAGTC CCGACCAAAG GAACGTAGGG TGTTTCTCTT TGAGCAAATA 
GTTATCTTCA GCGAGCTGCT TAGGAAAGGG TCTTTAACAC CAGGCTACAT GTTCAAGAGA 
AGCATAAAGA TGAACTACCT TGTCATTGAA GAAAATGTGG ATGGTGATCC CTGCAAGTTT 
GCTCTAATGA ATCGGGAAAC ATCTGAGAAA GTTGTTTTAC AGGCAGCTAA TCCAGAAATT 
CAGCAGGCTT GGGTACAGGA CATCAACCAG GTCCTGGACA CACAAAGGGA TTTCCTAAAT 
GCACTGCAGT CTCCCATAGA GTACCAACGC AAAGAAAGTA GCACAGCAGT GCTGAGGCCC 
CAGGCTGGTA GGGTCGCTCA GCCCAACAGC AGACCCTATT CTTCTGTTCC AGTAGGGTCT 
GAGAAGCCTT TGAAGGCTAC AAGCCGTAAC CCATCTCTCC CACCCCTGAA GATATCTACC 
TCCAACGGCA GTACAGGGTA TGAACAGCCC GGAGACAAGT ATGAACAAAG CAAGACTGAT 
TTGGGAGGGT GCAATGGGAC ATCTTCCATG GTGGTGATTA AAGATTACTA TGCAGTGAAG 
GAGGACGAGA TCTGTGTGAA TCAAGGAGAG GTGGTTCAGA TCCTTGCTAT CAACCAGCAA 
AACATGTTCC TGGTGTACCA GCCTGCAAAT GACCACTCTC CAGCTGCTGA AGGATGGATC 
CCTGGCAATA TCTTGGCTCC CCTCACTAAA CCCACTACAG ATAATAGCGA CGGAAGCATC 
AAGAAGTCAT GTTCATGGCA TACCCTGCGC ATGAGAAAGC GGGCGGAAAA GGAGAGCAGT 
GGCAAAAATG AAGCCAATGG GCCACGTAAA TCCAAGGATA TTCTGGGGAA CAAAGTCTCT 
GTTAAAGAGA CAAACAGCTC AGAGGAATCA GAGTGTGATG ACCTTGACCC CAATACTAGC 
ATGGAGATAA TCAACCCCAA TTTCATTCAG GAAGTGGCTC CAGAGTTCCT TGTGCTGTTG 
GTGGATATCA CCTGCCTGCT TGGCGACACT GTGATGCTGC AGTGCAAAGT CTGCGGCCGG 
CCCAAACCAA CAATAACCTG GAAAGGACCT GATCAAAACA TTCTTGACAA TGACAACAGC 
ACGGCCACAT ACACGGTGTC ATCCTGTGAC TCTGGAGAGC TCATGCTGAA GATCTGCAAT 
GTGATGCCTC AGGACAGTGG CATTTACACT TGTGTGGCAA CTAACGAGCA TGGAACGGCA 
TCAACCTCAG CCACAATCAA AGTGCAAGGT GTACCGGCTG CCCCAAATCG CCCCATTGCT 
CAGGAGAGAA GCTGCACCTC TGTGATCCTT CGCTGGCTGC CTCCATCCAG TACAGGCAAT 
TGCACCATTT CTGGCTACAC AGTGGAGTAC AGAGAAGAAG GCTCACAGGT CTGGCAGCAG 
TCAGTGGCCT CCACACTGGA CACTTACCTT GTCATTGAAG ACCTGTCCCC AGGCTGCCAG 
TATCAGTTCC GTGTGAGTGC CAGCAACCCC TGGGGGATCA GCTTGCCCAG TGACCCATCT 
GAATTCGTGA GGCTTCCAGA GTATGATTCT GCTGCTGATG GTGCCACTAT TTCGTGGAAG 
GAAAACTTTG ATCTCGCTTA TGCGGAGCTG CATGAGATTG GGAGGGGACG ATTCTCCATT 
GTAAAGAAAT GCGTTCACAA AGCTACCCGG AAAGACGTGG CTGTAAAATT CATCAGTAAA 
AAAATGAAGA AGAAGGAGCA AGCAGCCCAC GAGGCAGCTC TGTTACAGCA CTTACAGCAT 
CCTCAGTACA TCACTATCCA TGATACCTAT GAGTCTCCTA CTTCTTACAT CCTAGTCCTG 
GAGCTGATGG ATGATGGTCG TCTCCTAGAT TATCTAATGA ACCATGATGA ACTTATGGAG 
GAAAAAGTAG CTTTCTACAT AAGAGACACA ATGGAAGCCT TGCAGTATCT TCACAATTGC 
CGAGTGGCTC ATTTAGATAT AAAGCCAGAG AATCTGCTCA TCGATTTAAG GATCCCAGTT 
CCTCGTGTCA AGATCATTGA TTTGGAAGAT GCAGTTCAAA TCACAGGCCA TTACCACGTC 
CACCATCTCC TTGGAAACCC AGAGTTTGCA GCCCCTGAAG TTATCCAGGG CCTCCCCGTG 
TCCCTGAGCA CAGACATCTG GAGCATCGGG GTCCTCACCT ACGTCATGCT GAGCGGTGTC 
TCCCCGTTCC TGGATGAAAG CAAAGAGGAG ACGTGTATCA ATGTGTGCAG AGTCGATTTC 
AGCTTCCCGC CCGAGTACTT CTCTGATGTG AGCCATGCTG CTAGGGATTT CATCAACGTC 
ATCCTCCAGG AAGACTCCAG GAGGAGGCCG ACAGCAGCCA CTTGTTTACA GCACCCATGG 
CTGCAGCCAC ACAATGGCAG CTACTCCAAA ATCCCGCTGG ATACCTCCCG CTTGGCATCC 
TTCATTGAGC GCCGCAGACA CCAGTACGAC GTGCACCCCG TCCCCGGTGT CAAGAGTTTC 
CTGATGAGCA GGCTGAACCC CGGGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145467.1
CDS	233..9082
Translation	MNPTEGAAEEGAVPDSEVDAFFRTGSFRNDGLKAADVLPILKEKVAFVSGGRDKRGGPVLTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLAKLVDPSQLTDEFEGSLDYNHDEWIELRVSLEEFFNSAIHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVIKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKELLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAVSNNKTPHNSSISHIESVLQQLDEAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDIDLATQVQELLEFLHEKQHELELNADQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEVLLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEIDHVIPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPGVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHNSTGESAEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKRYRDFSLRMGKYRYSLEKALGINTEDNKDLELDIIPASLSDREVKLRDANHEVNEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPAGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSSQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDSFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASSIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKQRNNSRRDGVDDADSQGDGSSQPDTISIASRTSQNTVDSDKHFVYGRDTGQLSGGCELTVVLQDFTAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPSGKEAYSVSSNENGGKSDSVANLQPQPSLNSIQSSPGPKRSTNTLKKWLTSPVRRLNSGKAESNIKKQKKVRDGRKSFDLGSPKTGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMTLEGGSYRGSLKDATGCLNEGMAPSTPPRNLEEEQKAKAMRGRMFVLNELVQTEKDYVKDLGTVVEGFMKRIEEKGVSEDMKGKDKIVFGNIHQIYDWHKDFFLAELEKCLQEPDRLAQLFIKHERRLHMYVVYCQNKPRSEYIVAEYEAYFEEVQQEINQRLTISDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSEIEKAVELMCLVPKRCNDMMNLGRLQGFEGKLTAQGKLLQQDTFYVTEHDSGVQSRPKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVIEENVDGDPCKFALMNRETSEKVVLQAANPEIQQAWVQDINQVLDTQRDFLNALQSPIEYQRKESSTAVLRPQAGRVAQPNSRPYSSVPVGSEKPLKATSRNPSLPPLKISTSNGSTGYEQPGDKYEQSKTDLGGCNGTSSMVVIKDYYAVKEDEICVNQGEVVQILAINQQNMFLVYQPANDHSPAAEGWIPGNILAPLTKPTTDNSDGSIKKSCSWHTLRMRKRAEKESSGKNEANGPRKSKDILGNKVSVKETNSSEESECDDLDPNTSMEIINPNFIQEVAPEFLVLLVDITCLLGDTVMLQCKVCGRPKPTITWKGPDQNILDNDNSTATYTVSSCDSGELMLKICNVMPQDSGIYTCVATNEHGTASTSATIKVQGVPAAPNRPIAQERSCTSVILRWLPPSSTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCQYQFRVSASNPWGISLPSDPSEFVRLPEYDSAADGATISWKENFDLAYAELHEIGRGRFSIVKKCVHKATRKDVAVKFISKKMKKKEQAAHEAALLQHLQHPQYITIHDTYESPTSYILVLELMDDGRLLDYLMNHDELMEEKVAFYIRDTMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKIIDLEDAVQITGHYHVHHLLGNPEFAAPEVIQGLPVSLSTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPPEYFSDVSHAARDFINVILQEDSRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLASFIERRRHQYDVHPVPGVKSFLMSRLNPGT

Target ORF information:

RefSeq Version	XM_015289981.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289981.1

ORF Insert Sequence:

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGCATT TCGTCTACGG CAGAGACACA 
GGACAGTTGT CTGGAGGGTG TGAACTAACC GTGGTGCTCC AGGACTTCAC GGCGGGCCAC 
AGCAGCGAGC TGAGCATTCA GGTGGGGCAG ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA 
AGGCCAGGCT GGTGCTTGGT GCGGACCACG GAGCGGAGCC CACCTCAGGA AGGCCTGGTC 
CCCAGCAGCG CTCTCTGCAT TTCCCATTCC CGCAGCAGCG TGGAGATGGA TTGCTTCTTC 
CCTTCAGGAA AAGAGGCATA TTCAGTCTCT TCCAATGAAA ACGGGGGCAA GTCCGATTCA 
GTGGCCAACT TACAGCCCCA GCCATCCCTC AACTCCATCC AGAGCTCTCC CGGCCCCAAG 
CGCTCGACCA ACACGCTGAA GAAATGGCTG ACGAGCCCCG TGCGCCGGCT GAACAGCGGG 
AAGGCCGAGA GCAACATCAA GAAGCAAAAG AAGGTGCGAG ATGGCAGGAA GAGCTTTGAC 
CTCGGCTCGC CGAAGACTGG AGATGAAACC ACTCCTCAAG GAGACAGCGC GGATGAGAAG 
AGCAAGAAGG GTTGGGGAGA AGATGAGCCA GATGAAGAGT CTCACACACC TCTTCCTCCT 
CCTATGAAGA TTTTTGACAA TGATCCAACC CAAGATGAGA TGACCCTTGA AGGAGGATCT 
TACCGAGGAA GCTTAAAAGA TGCCACAGGC TGCTTGAATG AAGGAATGGC GCCTTCAACT 
CCCCCAAGAA ACCTTGAAGA GGAACAAAAA GCCAAAGCAA TGAGAGGCAG GATGTTTGTC 
TTAAATGAAC TGGTCCAGAC TGAAAAAGAC TACGTCAAAG ACTTGGGAAC CGTTGTGGAG 
GGCTTCATGA AGAGAATAGA AGAAAAGGGA GTTTCCGAAG ATATGAAAGG GAAAGACAAG 
ATAGTGTTTG GCAATATTCA CCAGATCTAT GACTGGCACA AAGACTTTTT CCTGGCTGAA 
CTGGAAAAAT GTCTTCAGGA GCCTGACCGT TTAGCACAGC TTTTTATTAA ACATGAGCGG 
CGATTACACA TGTATGTGGT GTACTGCCAA AACAAGCCAC GATCTGAATA TATAGTAGCT 
GAATACGAGG CATATTTTGA GGAGGTTCAA CAGGAAATAA ACCAACGGCT GACCATCAGT 
GACTTTCTGA TCAAACCCAT TCAGAGAATA ACTAAATATC AGCTTCTTCT CAAGGACTTC 
CTGAGGTACA GTGAAAAAGC TGGTCTGGAG TGCTCTGAAA TAGAGAAAGC AGTTGAGTTA 
ATGTGCCTTG TACCAAAACG TTGCAATGAT ATGATGAACC TGGGTCGCCT CCAAGGCTTT 
GAGGGTAAGC TGACAGCCCA GGGCAAGCTG CTGCAGCAGG ACACCTTCTA CGTGACAGAG 
CATGACTCCG GAGTTCAGTC CCGACCAAAG GAACGTAGGG TGTTTCTCTT TGAGCAAATA 
GTTATCTTCA GCGAGCTGCT TAGGAAAGGG TCTTTAACAC CAGGCTACAT GTTCAAGAGA 
AGCATAAAGA TGAACTACCT TGTCATTGAA GAAAATGTGG ATGGTGATCC CTGCAAGTTT 
GCTCTAATGA ATCGGGAAAC ATCTGAGAAA GTTGTTTTAC AGGCAGCTAA TCCAGAAATT 
CAGCAGGCTT GGGTACAGGA CATCAACCAG GTCCTGGACA CACAAAGGGA TTTCCTAAAT 
GCACTGCAGT CTCCCATAGA GTACCAACGC AAAGAAAGTA GCACAGCAGT GCTGAGGCCC 
CAGGCTGGTA GGGTCGCTCA GCCCAACAGC AGACCCTATT CTTCTGTTCC AGTAGGGTCT 
GAGAAGCCTT TGAAGGCTAC AAGCCGTAAC CCATCTCTCC CACCCCTGAA GATATCTACC 
TCCAACGGCA GTACAGGGTA TGAACAGCCC GGAGACAAGT ATGAACAAAG CAAGACTGAT 
TTGGGAGGGT GCAATGGGAC ATCTTCCATG GTGGTGATTA AAGATTACTA TGCAGTGAAG 
GAGGACGAGA TCTGTGTGAA TCAAGGAGAG GTGGTTCAGA TCCTTGCTAT CAACCAGCAA 
AACATGTTCC TGGTGTACCA GCCTGCAAAT GACCACTCTC CAGCTGCTGA AGGATGGATC 
CCTGGCAATA TCTTGGCTCC CCTCACTAAA CCCACTACAG ATAATAGCGA CGGAAGCATC 
AAGAAGTCAT GTTCATGGCA TACCCTGCGC ATGAGAAAGC GGGCGGAAAA GGAGAGCAGT 
GGCAAAAATG AAGCCAATGG GCCACGTAAA TCCAAGGATA TTCTGGGGAA CAAAGTCTCT 
GTTAAAGAGA CAAACAGCTC AGAGGAATCA GAGTGTGATG ACCTTGACCC CAATACTAGC 
ATGGAGATAA TCAACCCCAA TTTCATTCAG GAAGTGGCTC CAGAGTTCCT TGTGCTGTTG 
GTGGATATCA CCTGCCTGCT TGGCGACACT GTGATGCTGC AGTGCAAAGT CTGCGGCCGG 
CCCAAACCAA CAATAACCTG GAAAGGACCT GATCAAAACA TTCTTGACAA TGACAACAGC 
ACGGCCACAT ACACGGTGTC ATCCTGTGAC TCTGGAGAGC TCATGCTGAA GATCTGCAAT 
GTGATGCCTC AGGACAGTGG CATTTACACT TGTGTGGCAA CTAACGAGCA TGGAACGGCA 
TCAACCTCAG CCACAATCAA AGTGCAAGGT GTACCGGCTG CCCCAAATCG CCCCATTGCT 
CAGGAGAGAA GCTGCACCTC TGTGATCCTT CGCTGGCTGC CTCCATCCAG TACAGGCAAT 
TGCACCATTT CTGGCTACAC AGTGGAGTAC AGAGAAGAAG GCTCACAGGT CTGGCAGCAG 
TCAGTGGCCT CCACACTGGA CACTTACCTT GTCATTGAAG ACCTGTCCCC AGGCTGCCAG 
TATCAGTTCC GTGTGAGTGC CAGCAACCCC TGGGGGATCA GCTTGCCCAG TGACCCATCT 
GAATTCGTGA GGCTTCCAGA GTATGATTCT GCTGCTGATG GTGCCACTAT TTCGTGGAAG 
GAAAACTTTG ATCTCGCTTA TGCGGAGCTG CATGAGATTG GGAGGGGACG ATTCTCCATT 
GTAAAGAAAT GCGTTCACAA AGCTACCCGG AAAGACGTGG CTGTAAAATT CATCAGTAAA 
AAAATGAAGA AGAAGGAGCA AGCAGCCCAC GAGGCAGCTC TGTTACAGCA CTTACAGCAT 
CCTCAGTACA TCACTATCCA TGATACCTAT GAGTCTCCTA CTTCTTACAT CCTAGTCCTG 
GAGCTGATGG ATGATGGTCG TCTCCTAGAT TATCTAATGA ACCATGATGA ACTTATGGAG 
GAAAAAGTAG CTTTCTACAT AAGAGACACA ATGGAAGCCT TGCAGTATCT TCACAATTGC 
CGAGTGGCTC ATTTAGATAT AAAGCCAGAG AATCTGCTCA TCGATTTAAG GATCCCAGTT 
CCTCGTGTCA AGATCATTGA TTTGGAAGAT GCAGTTCAAA TCACAGGCCA TTACCACGTC 
CACCATCTCC TTGGAAACCC AGAGTTTGCA GCCCCTGAAG TTATCCAGGG CCTCCCCGTG 
TCCCTGAGCA CAGACATCTG GAGCATCGGG GTCCTCACCT ACGTCATGCT GAGCGGTGTC 
TCCCCGTTCC TGGATGAAAG CAAAGAGGAG ACGTGTATCA ATGTGTGCAG AGTCGATTTC 
AGCTTCCCGC CCGAGTACTT CTCTGATGTG AGCCATGCTG CTAGGGATTT CATCAACGTC 
ATCCTCCAGG AAGACTCCAG GAGGAGGCCG ACAGCAGCCA CTTGTTTACA GCACCCATGG 
CTGCAGCCAC ACAATGGCAG CTACTCCAAA ATCCCGCTGG ATACCTCCCG CTTGGCATCC 
TTCATTGAGC GCCGCAGACA CCAGTACGAC GTGCACCCCG TCCCCGGTGT CAAGAGTTTC 
CTGATGAGCA GGCTGAACCC CGGGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KALRN ( XM_015289982.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145468.1 Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X7, mRNA.

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CloneID	OGa38678
Clone ID Related Accession (Same CDS sequence)	XM_015289982.2 , XM_015289982.1
Accession Version	XM_015289982.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	kalirin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289982.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGCATT TCGTCTACGG CAGAGACACA 
GGACAGTTGT CTGGAGGGTG TGAACTAACC GTGGTGCTCC AGGACTTCAC GGCGGGCCAC 
AGCAGCGAGC TGAGCATTCA GGTGGGGCAG ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA 
AGGCCAGGCT GGTGCTTGGT GCGGACCACG GAGCGGAGCC CACCTCAGGA AGGCCTGGTC 
CCCAGCAGCG CTCTCTGCAT TTCCCATTCC CGCAGCAGCG TGGAGATGGA TTGCTTCTTC 
CCTTCAGGAA AAGAGGCATA TTCAGTCTCT TCCAATGAAA ACGGGGGCAA GTCCGATTCA 
GTGGCCAACT TACAGCCCCA GCCATCCCTC AACTCCATCC AGAGCTCTCC CGGCCCCAAG 
CGCTCGACCA ACACGCTGAA GAAATGGCTG ACGAGCCCCG TGCGCCGGCT GAACAGCGGG 
AAGGCCGAGA GCAACATCAA GAAGCAAAAG AAGGTGCGAG ATGGCAGGAA GAGCTTTGAC 
CTCGGCTCGC CGAAGACTGG AGATGAAACC ACTCCTCAAG GAGACAGCGC GGATGAGAGC 
AAGAAGGGTT GGGGAGAAGA TGAGCCAGAT GAAGAGTCTC ACACACCTCT TCCTCCTCCT 
ATGAAGATTT TTGACAATGA TCCAACCCAA GATGAGATGA CCCTTGAAGG AGGATCTTAC 
CGAGGAAGCT TAAAAGATGC CACAGGCTGC TTGAATGAAG GAATGGCGCC TTCAACTCCC 
CCAAGAAACC TTGAAGAGGA ACAAAAAGCC AAAGCAATGA GAGGCAGGAT GTTTGTCTTA 
AATGAACTGG TCCAGACTGA AAAAGACTAC GTCAAAGACT TGGGAACCGT TGTGGAGGGC 
TTCATGAAGA GAATAGAAGA AAAGGGAGTT TCCGAAGATA TGAAAGGGAA AGACAAGATA 
GTGTTTGGCA ATATTCACCA GATCTATGAC TGGCACAAAG ACTTTTTCCT GGCTGAACTG 
GAAAAATGTC TTCAGGAGCC TGACCGTTTA GCACAGCTTT TTATTAAACA TGAGCGGCGA 
TTACACATGT ATGTGGTGTA CTGCCAAAAC AAGCCACGAT CTGAATATAT AGTAGCTGAA 
TACGAGGCAT ATTTTGAGGA GGTTCAACAG GAAATAAACC AACGGCTGAC CATCAGTGAC 
TTTCTGATCA AACCCATTCA GAGAATAACT AAATATCAGC TTCTTCTCAA GGACTTCCTG 
AGGTACAGTG AAAAAGCTGG TCTGGAGTGC TCTGAAATAG AGAAAGCAGT TGAGTTAATG 
TGCCTTGTAC CAAAACGTTG CAATGATATG ATGAACCTGG GTCGCCTCCA AGGCTTTGAG 
GGTAAGCTGA CAGCCCAGGG CAAGCTGCTG CAGCAGGACA CCTTCTACGT GACAGAGCAT 
GACTCCGGAG TTCAGTCCCG ACCAAAGGAA CGTAGGGTGT TTCTCTTTGA GCAAATAGTT 
ATCTTCAGCG AGCTGCTTAG GAAAGGGTCT TTAACACCAG GCTACATGTT CAAGAGAAGC 
ATAAAGATGA ACTACCTTGT CATTGAAGAA AATGTGGATG GTGATCCCTG CAAGTTTGCT 
CTAATGAATC GGGAAACATC TGAGAAAGTT GTTTTACAGG CAGCTAATCC AGAAATTCAG 
CAGGCTTGGG TACAGGACAT CAACCAGGTC CTGGACACAC AAAGGGATTT CCTAAATGCA 
CTGCAGTCTC CCATAGAGTA CCAACGCAAA GAAAGTAGCA CAGCAGTGCT GAGGCCCCAG 
GCTGGTAGGG TCGCTCAGCC CAACAGCAGA CCCTATTCTT CTGTTCCAGT AGGGTCTGAG 
AAGCCTTTGA AGGCTACAAG CCGTAACCCA TCTCTCCCAC CCCTGAAGAT ATCTACCTCC 
AACGGCAGTA CAGGGTATGA ACAGCCCGGA GACAAGTATG AACAAAGCAA GACTGATTTG 
GGAGGGTGCA ATGGGACATC TTCCATGGTG GTGATTAAAG ATTACTATGC AGTGAAGGAG 
GACGAGATCT GTGTGAATCA AGGAGAGGTG GTTCAGATCC TTGCTATCAA CCAGCAAAAC 
ATGTTCCTGG TGTACCAGCC TGCAAATGAC CACTCTCCAG CTGCTGAAGG ATGGATCCCT 
GGCAATATCT TGGCTCCCCT CACTAAACCC ACTACAGATA ATAGCGACGG AAGCATCAAG 
AAGTCATGTT CATGGCATAC CCTGCGCATG AGAAAGCGGG CGGAAAAGGA GAGCAGTGGC 
AAAAATGAAG CCAATGGGCC ACGTAAATCC AAGGATATTC TGGGGAACAA AGTCTCTGTT 
AAAGAGACAA ACAGCTCAGA GGAATCAGAG TGTGATGACC TTGACCCCAA TACTAGCATG 
GAGATAATCA ACCCCAATTT CATTCAGGAA GTGGCTCCAG AGTTCCTTGT GCTGTTGGTG 
GATATCACCT GCCTGCTTGG CGACACTGTG ATGCTGCAGT GCAAAGTCTG CGGCCGGCCC 
AAACCAACAA TAACCTGGAA AGGACCTGAT CAAAACATTC TTGACAATGA CAACAGCACG 
GCCACATACA CGGTGTCATC CTGTGACTCT GGAGAGCTCA TGCTGAAGAT CTGCAATGTG 
ATGCCTCAGG ACAGTGGCAT TTACACTTGT GTGGCAACTA ACGAGCATGG AACGGCATCA 
ACCTCAGCCA CAATCAAAGT GCAAGGTGTA CCGGCTGCCC CAAATCGCCC CATTGCTCAG 
GAGAGAAGCT GCACCTCTGT GATCCTTCGC TGGCTGCCTC CATCCAGTAC AGGCAATTGC 
ACCATTTCTG GCTACACAGT GGAGTACAGA GAAGAAGGCT CACAGGTCTG GCAGCAGTCA 
GTGGCCTCCA CACTGGACAC TTACCTTGTC ATTGAAGACC TGTCCCCAGG CTGCCAGTAT 
CAGTTCCGTG TGAGTGCCAG CAACCCCTGG GGGATCAGCT TGCCCAGTGA CCCATCTGAA 
TTCGTGAGGC TTCCAGAGTA TGATTCTGCT GCTGATGGTG CCACTATTTC GTGGAAGGAA 
AACTTTGATC TCGCTTATGC GGAGCTGCAT GAGATTGGGA GGGGACGATT CTCCATTGTA 
AAGAAATGCG TTCACAAAGC TACCCGGAAA GACGTGGCTG TAAAATTCAT CAGTAAAAAA 
ATGAAGAAGA AGGAGCAAGC AGCCCACGAG GCAGCTCTGT TACAGCACTT ACAGCATCCT 
CAGTACATCA CTATCCATGA TACCTATGAG TCTCCTACTT CTTACATCCT AGTCCTGGAG 
CTGATGGATG ATGGTCGTCT CCTAGATTAT CTAATGAACC ATGATGAACT TATGGAGGAA 
AAAGTAGCTT TCTACATAAG AGACACAATG GAAGCCTTGC AGTATCTTCA CAATTGCCGA 
GTGGCTCATT TAGATATAAA GCCAGAGAAT CTGCTCATCG ATTTAAGGAT CCCAGTTCCT 
CGTGTCAAGA TCATTGATTT GGAAGATGCA GTTCAAATCA CAGGCCATTA CCACGTCCAC 
CATCTCCTTG GAAACCCAGA GTTTGCAGCC CCTGAAGTTA TCCAGGGCCT CCCCGTGTCC 
CTGAGCACAG ACATCTGGAG CATCGGGGTC CTCACCTACG TCATGCTGAG CGGTGTCTCC 
CCGTTCCTGG ATGAAAGCAA AGAGGAGACG TGTATCAATG TGTGCAGAGT CGATTTCAGC 
TTCCCGCCCG AGTACTTCTC TGATGTGAGC CATGCTGCTA GGGATTTCAT CAACGTCATC 
CTCCAGGAAG ACTCCAGGAG GAGGCCGACA GCAGCCACTT GTTTACAGCA CCCATGGCTG 
CAGCCACACA ATGGCAGCTA CTCCAAAATC CCGCTGGATA CCTCCCGCTT GGCATCCTTC 
ATTGAGCGCC GCAGACACCA GTACGACGTG CACCCCGTCC CCGGTGTCAA GAGTTTCCTG 
ATGAGCAGGC TGAACCCCGG GACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145468.1
CDS	244..9090
Translation	MNPTEGAAEEGAVPDSEVDAFFRTGSFRNDGLKAADVLPILKEKVAFVSGGRDKRGGPVLTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLAKLVDPSQLTDEFEGSLDYNHDEWIELRVSLEEFFNSAIHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVIKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKELLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAVSNNKTPHNSSISHIESVLQQLDEAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDIDLATQVQELLEFLHEKQHELELNADQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEVLLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEIDHVIPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPGVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHNSTGESAEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKRYRDFSLRMGKYRYSLEKALGINTEDNKDLELDIIPASLSDREVKLRDANHEVNEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPAGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSSQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDSFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASSIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKQRNNSRRDGVDDADSQGDGSSQPDTISIASRTSQNTVDSDKHFVYGRDTGQLSGGCELTVVLQDFTAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPSGKEAYSVSSNENGGKSDSVANLQPQPSLNSIQSSPGPKRSTNTLKKWLTSPVRRLNSGKAESNIKKQKKVRDGRKSFDLGSPKTGDETTPQGDSADESKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMTLEGGSYRGSLKDATGCLNEGMAPSTPPRNLEEEQKAKAMRGRMFVLNELVQTEKDYVKDLGTVVEGFMKRIEEKGVSEDMKGKDKIVFGNIHQIYDWHKDFFLAELEKCLQEPDRLAQLFIKHERRLHMYVVYCQNKPRSEYIVAEYEAYFEEVQQEINQRLTISDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSEIEKAVELMCLVPKRCNDMMNLGRLQGFEGKLTAQGKLLQQDTFYVTEHDSGVQSRPKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVIEENVDGDPCKFALMNRETSEKVVLQAANPEIQQAWVQDINQVLDTQRDFLNALQSPIEYQRKESSTAVLRPQAGRVAQPNSRPYSSVPVGSEKPLKATSRNPSLPPLKISTSNGSTGYEQPGDKYEQSKTDLGGCNGTSSMVVIKDYYAVKEDEICVNQGEVVQILAINQQNMFLVYQPANDHSPAAEGWIPGNILAPLTKPTTDNSDGSIKKSCSWHTLRMRKRAEKESSGKNEANGPRKSKDILGNKVSVKETNSSEESECDDLDPNTSMEIINPNFIQEVAPEFLVLLVDITCLLGDTVMLQCKVCGRPKPTITWKGPDQNILDNDNSTATYTVSSCDSGELMLKICNVMPQDSGIYTCVATNEHGTASTSATIKVQGVPAAPNRPIAQERSCTSVILRWLPPSSTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCQYQFRVSASNPWGISLPSDPSEFVRLPEYDSAADGATISWKENFDLAYAELHEIGRGRFSIVKKCVHKATRKDVAVKFISKKMKKKEQAAHEAALLQHLQHPQYITIHDTYESPTSYILVLELMDDGRLLDYLMNHDELMEEKVAFYIRDTMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKIIDLEDAVQITGHYHVHHLLGNPEFAAPEVIQGLPVSLSTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPPEYFSDVSHAARDFINVILQEDSRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLASFIERRRHQYDVHPVPGVKSFLMSRLNPGT

Target ORF information:

RefSeq Version	XM_015289982.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289982.2

ORF Insert Sequence:

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGCATT TCGTCTACGG CAGAGACACA 
GGACAGTTGT CTGGAGGGTG TGAACTAACC GTGGTGCTCC AGGACTTCAC GGCGGGCCAC 
AGCAGCGAGC TGAGCATTCA GGTGGGGCAG ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA 
AGGCCAGGCT GGTGCTTGGT GCGGACCACG GAGCGGAGCC CACCTCAGGA AGGCCTGGTC 
CCCAGCAGCG CTCTCTGCAT TTCCCATTCC CGCAGCAGCG TGGAGATGGA TTGCTTCTTC 
CCTTCAGGAA AAGAGGCATA TTCAGTCTCT TCCAATGAAA ACGGGGGCAA GTCCGATTCA 
GTGGCCAACT TACAGCCCCA GCCATCCCTC AACTCCATCC AGAGCTCTCC CGGCCCCAAG 
CGCTCGACCA ACACGCTGAA GAAATGGCTG ACGAGCCCCG TGCGCCGGCT GAACAGCGGG 
AAGGCCGAGA GCAACATCAA GAAGCAAAAG AAGGTGCGAG ATGGCAGGAA GAGCTTTGAC 
CTCGGCTCGC CGAAGACTGG AGATGAAACC ACTCCTCAAG GAGACAGCGC GGATGAGAGC 
AAGAAGGGTT GGGGAGAAGA TGAGCCAGAT GAAGAGTCTC ACACACCTCT TCCTCCTCCT 
ATGAAGATTT TTGACAATGA TCCAACCCAA GATGAGATGA CCCTTGAAGG AGGATCTTAC 
CGAGGAAGCT TAAAAGATGC CACAGGCTGC TTGAATGAAG GAATGGCGCC TTCAACTCCC 
CCAAGAAACC TTGAAGAGGA ACAAAAAGCC AAAGCAATGA GAGGCAGGAT GTTTGTCTTA 
AATGAACTGG TCCAGACTGA AAAAGACTAC GTCAAAGACT TGGGAACCGT TGTGGAGGGC 
TTCATGAAGA GAATAGAAGA AAAGGGAGTT TCCGAAGATA TGAAAGGGAA AGACAAGATA 
GTGTTTGGCA ATATTCACCA GATCTATGAC TGGCACAAAG ACTTTTTCCT GGCTGAACTG 
GAAAAATGTC TTCAGGAGCC TGACCGTTTA GCACAGCTTT TTATTAAACA TGAGCGGCGA 
TTACACATGT ATGTGGTGTA CTGCCAAAAC AAGCCACGAT CTGAATATAT AGTAGCTGAA 
TACGAGGCAT ATTTTGAGGA GGTTCAACAG GAAATAAACC AACGGCTGAC CATCAGTGAC 
TTTCTGATCA AACCCATTCA GAGAATAACT AAATATCAGC TTCTTCTCAA GGACTTCCTG 
AGGTACAGTG AAAAAGCTGG TCTGGAGTGC TCTGAAATAG AGAAAGCAGT TGAGTTAATG 
TGCCTTGTAC CAAAACGTTG CAATGATATG ATGAACCTGG GTCGCCTCCA AGGCTTTGAG 
GGTAAGCTGA CAGCCCAGGG CAAGCTGCTG CAGCAGGACA CCTTCTACGT GACAGAGCAT 
GACTCCGGAG TTCAGTCCCG ACCAAAGGAA CGTAGGGTGT TTCTCTTTGA GCAAATAGTT 
ATCTTCAGCG AGCTGCTTAG GAAAGGGTCT TTAACACCAG GCTACATGTT CAAGAGAAGC 
ATAAAGATGA ACTACCTTGT CATTGAAGAA AATGTGGATG GTGATCCCTG CAAGTTTGCT 
CTAATGAATC GGGAAACATC TGAGAAAGTT GTTTTACAGG CAGCTAATCC AGAAATTCAG 
CAGGCTTGGG TACAGGACAT CAACCAGGTC CTGGACACAC AAAGGGATTT CCTAAATGCA 
CTGCAGTCTC CCATAGAGTA CCAACGCAAA GAAAGTAGCA CAGCAGTGCT GAGGCCCCAG 
GCTGGTAGGG TCGCTCAGCC CAACAGCAGA CCCTATTCTT CTGTTCCAGT AGGGTCTGAG 
AAGCCTTTGA AGGCTACAAG CCGTAACCCA TCTCTCCCAC CCCTGAAGAT ATCTACCTCC 
AACGGCAGTA CAGGGTATGA ACAGCCCGGA GACAAGTATG AACAAAGCAA GACTGATTTG 
GGAGGGTGCA ATGGGACATC TTCCATGGTG GTGATTAAAG ATTACTATGC AGTGAAGGAG 
GACGAGATCT GTGTGAATCA AGGAGAGGTG GTTCAGATCC TTGCTATCAA CCAGCAAAAC 
ATGTTCCTGG TGTACCAGCC TGCAAATGAC CACTCTCCAG CTGCTGAAGG ATGGATCCCT 
GGCAATATCT TGGCTCCCCT CACTAAACCC ACTACAGATA ATAGCGACGG AAGCATCAAG 
AAGTCATGTT CATGGCATAC CCTGCGCATG AGAAAGCGGG CGGAAAAGGA GAGCAGTGGC 
AAAAATGAAG CCAATGGGCC ACGTAAATCC AAGGATATTC TGGGGAACAA AGTCTCTGTT 
AAAGAGACAA ACAGCTCAGA GGAATCAGAG TGTGATGACC TTGACCCCAA TACTAGCATG 
GAGATAATCA ACCCCAATTT CATTCAGGAA GTGGCTCCAG AGTTCCTTGT GCTGTTGGTG 
GATATCACCT GCCTGCTTGG CGACACTGTG ATGCTGCAGT GCAAAGTCTG CGGCCGGCCC 
AAACCAACAA TAACCTGGAA AGGACCTGAT CAAAACATTC TTGACAATGA CAACAGCACG 
GCCACATACA CGGTGTCATC CTGTGACTCT GGAGAGCTCA TGCTGAAGAT CTGCAATGTG 
ATGCCTCAGG ACAGTGGCAT TTACACTTGT GTGGCAACTA ACGAGCATGG AACGGCATCA 
ACCTCAGCCA CAATCAAAGT GCAAGGTGTA CCGGCTGCCC CAAATCGCCC CATTGCTCAG 
GAGAGAAGCT GCACCTCTGT GATCCTTCGC TGGCTGCCTC CATCCAGTAC AGGCAATTGC 
ACCATTTCTG GCTACACAGT GGAGTACAGA GAAGAAGGCT CACAGGTCTG GCAGCAGTCA 
GTGGCCTCCA CACTGGACAC TTACCTTGTC ATTGAAGACC TGTCCCCAGG CTGCCAGTAT 
CAGTTCCGTG TGAGTGCCAG CAACCCCTGG GGGATCAGCT TGCCCAGTGA CCCATCTGAA 
TTCGTGAGGC TTCCAGAGTA TGATTCTGCT GCTGATGGTG CCACTATTTC GTGGAAGGAA 
AACTTTGATC TCGCTTATGC GGAGCTGCAT GAGATTGGGA GGGGACGATT CTCCATTGTA 
AAGAAATGCG TTCACAAAGC TACCCGGAAA GACGTGGCTG TAAAATTCAT CAGTAAAAAA 
ATGAAGAAGA AGGAGCAAGC AGCCCACGAG GCAGCTCTGT TACAGCACTT ACAGCATCCT 
CAGTACATCA CTATCCATGA TACCTATGAG TCTCCTACTT CTTACATCCT AGTCCTGGAG 
CTGATGGATG ATGGTCGTCT CCTAGATTAT CTAATGAACC ATGATGAACT TATGGAGGAA 
AAAGTAGCTT TCTACATAAG AGACACAATG GAAGCCTTGC AGTATCTTCA CAATTGCCGA 
GTGGCTCATT TAGATATAAA GCCAGAGAAT CTGCTCATCG ATTTAAGGAT CCCAGTTCCT 
CGTGTCAAGA TCATTGATTT GGAAGATGCA GTTCAAATCA CAGGCCATTA CCACGTCCAC 
CATCTCCTTG GAAACCCAGA GTTTGCAGCC CCTGAAGTTA TCCAGGGCCT CCCCGTGTCC 
CTGAGCACAG ACATCTGGAG CATCGGGGTC CTCACCTACG TCATGCTGAG CGGTGTCTCC 
CCGTTCCTGG ATGAAAGCAA AGAGGAGACG TGTATCAATG TGTGCAGAGT CGATTTCAGC 
TTCCCGCCCG AGTACTTCTC TGATGTGAGC CATGCTGCTA GGGATTTCAT CAACGTCATC 
CTCCAGGAAG ACTCCAGGAG GAGGCCGACA GCAGCCACTT GTTTACAGCA CCCATGGCTG 
CAGCCACACA ATGGCAGCTA CTCCAAAATC CCGCTGGATA CCTCCCGCTT GGCATCCTTC 
ATTGAGCGCC GCAGACACCA GTACGACGTG CACCCCGTCC CCGGTGTCAA GAGTTTCCTG 
ATGAGCAGGC TGAACCCCGG GACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KALRN ( XM_015289982.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145468.1 Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X8, mRNA.

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CloneID	OGa38678
Clone ID Related Accession (Same CDS sequence)	XM_015289982.2 , XM_015289982.1
Accession Version	XM_015289982.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	kalirin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGCATT TCGTCTACGG CAGAGACACA 
GGACAGTTGT CTGGAGGGTG TGAACTAACC GTGGTGCTCC AGGACTTCAC GGCGGGCCAC 
AGCAGCGAGC TGAGCATTCA GGTGGGGCAG ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA 
AGGCCAGGCT GGTGCTTGGT GCGGACCACG GAGCGGAGCC CACCTCAGGA AGGCCTGGTC 
CCCAGCAGCG CTCTCTGCAT TTCCCATTCC CGCAGCAGCG TGGAGATGGA TTGCTTCTTC 
CCTTCAGGAA AAGAGGCATA TTCAGTCTCT TCCAATGAAA ACGGGGGCAA GTCCGATTCA 
GTGGCCAACT TACAGCCCCA GCCATCCCTC AACTCCATCC AGAGCTCTCC CGGCCCCAAG 
CGCTCGACCA ACACGCTGAA GAAATGGCTG ACGAGCCCCG TGCGCCGGCT GAACAGCGGG 
AAGGCCGAGA GCAACATCAA GAAGCAAAAG AAGGTGCGAG ATGGCAGGAA GAGCTTTGAC 
CTCGGCTCGC CGAAGACTGG AGATGAAACC ACTCCTCAAG GAGACAGCGC GGATGAGAGC 
AAGAAGGGTT GGGGAGAAGA TGAGCCAGAT GAAGAGTCTC ACACACCTCT TCCTCCTCCT 
ATGAAGATTT TTGACAATGA TCCAACCCAA GATGAGATGA CCCTTGAAGG AGGATCTTAC 
CGAGGAAGCT TAAAAGATGC CACAGGCTGC TTGAATGAAG GAATGGCGCC TTCAACTCCC 
CCAAGAAACC TTGAAGAGGA ACAAAAAGCC AAAGCAATGA GAGGCAGGAT GTTTGTCTTA 
AATGAACTGG TCCAGACTGA AAAAGACTAC GTCAAAGACT TGGGAACCGT TGTGGAGGGC 
TTCATGAAGA GAATAGAAGA AAAGGGAGTT TCCGAAGATA TGAAAGGGAA AGACAAGATA 
GTGTTTGGCA ATATTCACCA GATCTATGAC TGGCACAAAG ACTTTTTCCT GGCTGAACTG 
GAAAAATGTC TTCAGGAGCC TGACCGTTTA GCACAGCTTT TTATTAAACA TGAGCGGCGA 
TTACACATGT ATGTGGTGTA CTGCCAAAAC AAGCCACGAT CTGAATATAT AGTAGCTGAA 
TACGAGGCAT ATTTTGAGGA GGTTCAACAG GAAATAAACC AACGGCTGAC CATCAGTGAC 
TTTCTGATCA AACCCATTCA GAGAATAACT AAATATCAGC TTCTTCTCAA GGACTTCCTG 
AGGTACAGTG AAAAAGCTGG TCTGGAGTGC TCTGAAATAG AGAAAGCAGT TGAGTTAATG 
TGCCTTGTAC CAAAACGTTG CAATGATATG ATGAACCTGG GTCGCCTCCA AGGCTTTGAG 
GGTAAGCTGA CAGCCCAGGG CAAGCTGCTG CAGCAGGACA CCTTCTACGT GACAGAGCAT 
GACTCCGGAG TTCAGTCCCG ACCAAAGGAA CGTAGGGTGT TTCTCTTTGA GCAAATAGTT 
ATCTTCAGCG AGCTGCTTAG GAAAGGGTCT TTAACACCAG GCTACATGTT CAAGAGAAGC 
ATAAAGATGA ACTACCTTGT CATTGAAGAA AATGTGGATG GTGATCCCTG CAAGTTTGCT 
CTAATGAATC GGGAAACATC TGAGAAAGTT GTTTTACAGG CAGCTAATCC AGAAATTCAG 
CAGGCTTGGG TACAGGACAT CAACCAGGTC CTGGACACAC AAAGGGATTT CCTAAATGCA 
CTGCAGTCTC CCATAGAGTA CCAACGCAAA GAAAGTAGCA CAGCAGTGCT GAGGCCCCAG 
GCTGGTAGGG TCGCTCAGCC CAACAGCAGA CCCTATTCTT CTGTTCCAGT AGGGTCTGAG 
AAGCCTTTGA AGGCTACAAG CCGTAACCCA TCTCTCCCAC CCCTGAAGAT ATCTACCTCC 
AACGGCAGTA CAGGGTATGA ACAGCCCGGA GACAAGTATG AACAAAGCAA GACTGATTTG 
GGAGGGTGCA ATGGGACATC TTCCATGGTG GTGATTAAAG ATTACTATGC AGTGAAGGAG 
GACGAGATCT GTGTGAATCA AGGAGAGGTG GTTCAGATCC TTGCTATCAA CCAGCAAAAC 
ATGTTCCTGG TGTACCAGCC TGCAAATGAC CACTCTCCAG CTGCTGAAGG ATGGATCCCT 
GGCAATATCT TGGCTCCCCT CACTAAACCC ACTACAGATA ATAGCGACGG AAGCATCAAG 
AAGTCATGTT CATGGCATAC CCTGCGCATG AGAAAGCGGG CGGAAAAGGA GAGCAGTGGC 
AAAAATGAAG CCAATGGGCC ACGTAAATCC AAGGATATTC TGGGGAACAA AGTCTCTGTT 
AAAGAGACAA ACAGCTCAGA GGAATCAGAG TGTGATGACC TTGACCCCAA TACTAGCATG 
GAGATAATCA ACCCCAATTT CATTCAGGAA GTGGCTCCAG AGTTCCTTGT GCTGTTGGTG 
GATATCACCT GCCTGCTTGG CGACACTGTG ATGCTGCAGT GCAAAGTCTG CGGCCGGCCC 
AAACCAACAA TAACCTGGAA AGGACCTGAT CAAAACATTC TTGACAATGA CAACAGCACG 
GCCACATACA CGGTGTCATC CTGTGACTCT GGAGAGCTCA TGCTGAAGAT CTGCAATGTG 
ATGCCTCAGG ACAGTGGCAT TTACACTTGT GTGGCAACTA ACGAGCATGG AACGGCATCA 
ACCTCAGCCA CAATCAAAGT GCAAGGTGTA CCGGCTGCCC CAAATCGCCC CATTGCTCAG 
GAGAGAAGCT GCACCTCTGT GATCCTTCGC TGGCTGCCTC CATCCAGTAC AGGCAATTGC 
ACCATTTCTG GCTACACAGT GGAGTACAGA GAAGAAGGCT CACAGGTCTG GCAGCAGTCA 
GTGGCCTCCA CACTGGACAC TTACCTTGTC ATTGAAGACC TGTCCCCAGG CTGCCAGTAT 
CAGTTCCGTG TGAGTGCCAG CAACCCCTGG GGGATCAGCT TGCCCAGTGA CCCATCTGAA 
TTCGTGAGGC TTCCAGAGTA TGATTCTGCT GCTGATGGTG CCACTATTTC GTGGAAGGAA 
AACTTTGATC TCGCTTATGC GGAGCTGCAT GAGATTGGGA GGGGACGATT CTCCATTGTA 
AAGAAATGCG TTCACAAAGC TACCCGGAAA GACGTGGCTG TAAAATTCAT CAGTAAAAAA 
ATGAAGAAGA AGGAGCAAGC AGCCCACGAG GCAGCTCTGT TACAGCACTT ACAGCATCCT 
CAGTACATCA CTATCCATGA TACCTATGAG TCTCCTACTT CTTACATCCT AGTCCTGGAG 
CTGATGGATG ATGGTCGTCT CCTAGATTAT CTAATGAACC ATGATGAACT TATGGAGGAA 
AAAGTAGCTT TCTACATAAG AGACACAATG GAAGCCTTGC AGTATCTTCA CAATTGCCGA 
GTGGCTCATT TAGATATAAA GCCAGAGAAT CTGCTCATCG ATTTAAGGAT CCCAGTTCCT 
CGTGTCAAGA TCATTGATTT GGAAGATGCA GTTCAAATCA CAGGCCATTA CCACGTCCAC 
CATCTCCTTG GAAACCCAGA GTTTGCAGCC CCTGAAGTTA TCCAGGGCCT CCCCGTGTCC 
CTGAGCACAG ACATCTGGAG CATCGGGGTC CTCACCTACG TCATGCTGAG CGGTGTCTCC 
CCGTTCCTGG ATGAAAGCAA AGAGGAGACG TGTATCAATG TGTGCAGAGT CGATTTCAGC 
TTCCCGCCCG AGTACTTCTC TGATGTGAGC CATGCTGCTA GGGATTTCAT CAACGTCATC 
CTCCAGGAAG ACTCCAGGAG GAGGCCGACA GCAGCCACTT GTTTACAGCA CCCATGGCTG 
CAGCCACACA ATGGCAGCTA CTCCAAAATC CCGCTGGATA CCTCCCGCTT GGCATCCTTC 
ATTGAGCGCC GCAGACACCA GTACGACGTG CACCCCGTCC CCGGTGTCAA GAGTTTCCTG 
ATGAGCAGGC TGAACCCCGG GACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145468.1
CDS	233..9079
Translation	MNPTEGAAEEGAVPDSEVDAFFRTGSFRNDGLKAADVLPILKEKVAFVSGGRDKRGGPVLTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLAKLVDPSQLTDEFEGSLDYNHDEWIELRVSLEEFFNSAIHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVIKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKELLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAVSNNKTPHNSSISHIESVLQQLDEAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDIDLATQVQELLEFLHEKQHELELNADQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEVLLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEIDHVIPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPGVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHNSTGESAEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKRYRDFSLRMGKYRYSLEKALGINTEDNKDLELDIIPASLSDREVKLRDANHEVNEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPAGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSSQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDSFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASSIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKQRNNSRRDGVDDADSQGDGSSQPDTISIASRTSQNTVDSDKHFVYGRDTGQLSGGCELTVVLQDFTAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPSGKEAYSVSSNENGGKSDSVANLQPQPSLNSIQSSPGPKRSTNTLKKWLTSPVRRLNSGKAESNIKKQKKVRDGRKSFDLGSPKTGDETTPQGDSADESKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMTLEGGSYRGSLKDATGCLNEGMAPSTPPRNLEEEQKAKAMRGRMFVLNELVQTEKDYVKDLGTVVEGFMKRIEEKGVSEDMKGKDKIVFGNIHQIYDWHKDFFLAELEKCLQEPDRLAQLFIKHERRLHMYVVYCQNKPRSEYIVAEYEAYFEEVQQEINQRLTISDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSEIEKAVELMCLVPKRCNDMMNLGRLQGFEGKLTAQGKLLQQDTFYVTEHDSGVQSRPKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVIEENVDGDPCKFALMNRETSEKVVLQAANPEIQQAWVQDINQVLDTQRDFLNALQSPIEYQRKESSTAVLRPQAGRVAQPNSRPYSSVPVGSEKPLKATSRNPSLPPLKISTSNGSTGYEQPGDKYEQSKTDLGGCNGTSSMVVIKDYYAVKEDEICVNQGEVVQILAINQQNMFLVYQPANDHSPAAEGWIPGNILAPLTKPTTDNSDGSIKKSCSWHTLRMRKRAEKESSGKNEANGPRKSKDILGNKVSVKETNSSEESECDDLDPNTSMEIINPNFIQEVAPEFLVLLVDITCLLGDTVMLQCKVCGRPKPTITWKGPDQNILDNDNSTATYTVSSCDSGELMLKICNVMPQDSGIYTCVATNEHGTASTSATIKVQGVPAAPNRPIAQERSCTSVILRWLPPSSTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCQYQFRVSASNPWGISLPSDPSEFVRLPEYDSAADGATISWKENFDLAYAELHEIGRGRFSIVKKCVHKATRKDVAVKFISKKMKKKEQAAHEAALLQHLQHPQYITIHDTYESPTSYILVLELMDDGRLLDYLMNHDELMEEKVAFYIRDTMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKIIDLEDAVQITGHYHVHHLLGNPEFAAPEVIQGLPVSLSTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPPEYFSDVSHAARDFINVILQEDSRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLASFIERRRHQYDVHPVPGVKSFLMSRLNPGT

Target ORF information:

RefSeq Version	XM_015289982.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289982.1

ORF Insert Sequence:

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG TCCCTCTTTC ATGCCACTTC CTTACAGAAG 
ACACACCAGA GTGCCCTGCA GGTCCAGCAG AAAGCCGAAG TGCTGCTGCA GGCTGGGCAT 
TATGATGCTG ATGCCATCAG AGAATGCGCT GAAAAGGTGG CCCTGCACTG GCAGCAGCTG 
ATGCTGAAGA TGGAGGACAG GCTCAAGCTG GTCAATGCCT CGGTGGCCTT CTACAAAACC 
TCTGAGCAGG TGTGCAGTGT ATTAGAGAGC CTGGAGCAAG AATACAGACG TGATGAAGAC 
TGGTGTGGAG GTCGAGATAA ACTTGGACCA GCAGCTGAGA TAGACCACGT CATCCCTCTG 
ATCAGCAAGC ATCTGGAGCA GAAGGAGGCT TTTCTCAAGG CCTGCACACT GGCTCGAAGG 
AATGCTGAGG TATTCCTCAA GTACATCCAC AGGAACAATG TCAGCATGCC CGGAGTCGCA 
AGCCATACAC GGGGACCTGA GCAGCAAGTG AAAGCCATCC TGAGTGAGTT GCTGCAGAGG 
GAGAACCGGG TCCTTCACTT CTGGACCCTG AAGAAGCGGA GGTTAGATCA GTGCCAGCAG 
TACGTTGTAT TTGAGCGCAG TGCCAAGCAG GCCCTGGACT GGATCCAAGA GACAGGCGAA 
TATTACCTCT CTACTCACAA CTCTACAGGG GAATCAGCAG AAGAAACTCA GGAACTCCTG 
AAGGAATATG GGGAATTCAG AGTACCTGCG AAGCAAACAA AGGAGAAAGT GAAGCTCCTC 
ATCCAGCTGG CTGACAGCTT TGTAGAAAAA GGACATATAC ACGCCACTGA AATCAGGAAG 
TGGGTCACCA CCGTTGACAA ACGCTACCGT GACTTCTCTC TCAGGATGGG GAAATACCGC 
TACTCCCTGG AGAAAGCCCT TGGAATCAAT ACAGAGGATA ACAAGGACCT CGAACTAGAT 
ATTATTCCAG CAAGTCTTTC AGACCGGGAG GTAAAGCTGC GAGATGCAAA TCACGAAGTC 
AATGAAGAAA AAAGAAAGTC TGCTAGAAAG AAAGAATTCA TTATGGCCGA GCTTCTTCAG 
ACAGAGAAAG CTTATGTCAG GGACTTACAT GAATGTTTAG AGACTTACCT TTGGGAAATG 
ACAAGTGGGG TGGAAGAAAT ACCCGCTGGG ATCCTTAATA AAGAACACAT CATCTTTGGC 
AATATTCAGG AGATATATGA CTTCCATAAC AACATTTTCC TGAAAGAACT CGAGAAGTAT 
GAGCAACTGC CTGAGGATGT GGGTCACTGC TTTGTCACCT GGGCAGATAA ATTTCAGATG 
TATGTCACCT ACTGCAAAAA CAAGCCTGAC TCCAGCCAGC TCATCCTGGA ACACGCAGGG 
ACTTTCTTTG ATGAAATTCA GCAAAGGCAT GGTCTGGCCA ACTCTATCTC TTCTTATTTA 
ATCAAGCCTG TCCAGAGGAT CACAAAATAT CAACTCCTCC TGAAGGAACT GCTTACCTGC 
TGTGAGGAAG GCAAAGGGGA ACTCAAGGAT GGTCTGGAAG TGATGCTCAG TGTCCCCAAG 
AAAGCCAATG ATGCAATGCA CGTCAGCATG CTGGAAGGGT TTGATGAGAA TCTAGACGTC 
CAGGGAGAGC TGATTCTTCA GGATTCCTTC CAAGTGTGGG ACCCCAAGTC TCTCATTCGG 
AAAGGCCGTG AGAGACATTT GTTTCTCTTT GAAATCTCTT TGGTGTTTAG CAAAGAGATC 
AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGTTACTGAC CTCAGAACTG 
GGAGTGACTG AACATGTTGA AGGAGATCCC TGTAAATTTG CCTTGTGGTC TGGACGAACA 
CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCATCGAGTA TTGAAACAAA ACAGGAGTGG 
ATCAAAAATA TCCGAGAAGT CATCCAAGAA AGAATTATCC ATCTGAAAGG AGCTCTGAAA 
GAGCCAATTC AGCTCCCCAA GACACCAGCA AAGCAGCGAA ACAACAGTAG AAGAGATGGA 
GTAGATGATG CAGACAGCCA AGGAGATGGA AGCAGTCAAC CTGACACCAT TTCCATTGCA 
TCCAGGACAT CACAGAATAC AGTGGACAGT GATAAGCATT TCGTCTACGG CAGAGACACA 
GGACAGTTGT CTGGAGGGTG TGAACTAACC GTGGTGCTCC AGGACTTCAC GGCGGGCCAC 
AGCAGCGAGC TGAGCATTCA GGTGGGGCAG ACAGTGGAGC TGCTGGAGAG GCCAAGTGAA 
AGGCCAGGCT GGTGCTTGGT GCGGACCACG GAGCGGAGCC CACCTCAGGA AGGCCTGGTC 
CCCAGCAGCG CTCTCTGCAT TTCCCATTCC CGCAGCAGCG TGGAGATGGA TTGCTTCTTC 
CCTTCAGGAA AAGAGGCATA TTCAGTCTCT TCCAATGAAA ACGGGGGCAA GTCCGATTCA 
GTGGCCAACT TACAGCCCCA GCCATCCCTC AACTCCATCC AGAGCTCTCC CGGCCCCAAG 
CGCTCGACCA ACACGCTGAA GAAATGGCTG ACGAGCCCCG TGCGCCGGCT GAACAGCGGG 
AAGGCCGAGA GCAACATCAA GAAGCAAAAG AAGGTGCGAG ATGGCAGGAA GAGCTTTGAC 
CTCGGCTCGC CGAAGACTGG AGATGAAACC ACTCCTCAAG GAGACAGCGC GGATGAGAGC 
AAGAAGGGTT GGGGAGAAGA TGAGCCAGAT GAAGAGTCTC ACACACCTCT TCCTCCTCCT 
ATGAAGATTT TTGACAATGA TCCAACCCAA GATGAGATGA CCCTTGAAGG AGGATCTTAC 
CGAGGAAGCT TAAAAGATGC CACAGGCTGC TTGAATGAAG GAATGGCGCC TTCAACTCCC 
CCAAGAAACC TTGAAGAGGA ACAAAAAGCC AAAGCAATGA GAGGCAGGAT GTTTGTCTTA 
AATGAACTGG TCCAGACTGA AAAAGACTAC GTCAAAGACT TGGGAACCGT TGTGGAGGGC 
TTCATGAAGA GAATAGAAGA AAAGGGAGTT TCCGAAGATA TGAAAGGGAA AGACAAGATA 
GTGTTTGGCA ATATTCACCA GATCTATGAC TGGCACAAAG ACTTTTTCCT GGCTGAACTG 
GAAAAATGTC TTCAGGAGCC TGACCGTTTA GCACAGCTTT TTATTAAACA TGAGCGGCGA 
TTACACATGT ATGTGGTGTA CTGCCAAAAC AAGCCACGAT CTGAATATAT AGTAGCTGAA 
TACGAGGCAT ATTTTGAGGA GGTTCAACAG GAAATAAACC AACGGCTGAC CATCAGTGAC 
TTTCTGATCA AACCCATTCA GAGAATAACT AAATATCAGC TTCTTCTCAA GGACTTCCTG 
AGGTACAGTG AAAAAGCTGG TCTGGAGTGC TCTGAAATAG AGAAAGCAGT TGAGTTAATG 
TGCCTTGTAC CAAAACGTTG CAATGATATG ATGAACCTGG GTCGCCTCCA AGGCTTTGAG 
GGTAAGCTGA CAGCCCAGGG CAAGCTGCTG CAGCAGGACA CCTTCTACGT GACAGAGCAT 
GACTCCGGAG TTCAGTCCCG ACCAAAGGAA CGTAGGGTGT TTCTCTTTGA GCAAATAGTT 
ATCTTCAGCG AGCTGCTTAG GAAAGGGTCT TTAACACCAG GCTACATGTT CAAGAGAAGC 
ATAAAGATGA ACTACCTTGT CATTGAAGAA AATGTGGATG GTGATCCCTG CAAGTTTGCT 
CTAATGAATC GGGAAACATC TGAGAAAGTT GTTTTACAGG CAGCTAATCC AGAAATTCAG 
CAGGCTTGGG TACAGGACAT CAACCAGGTC CTGGACACAC AAAGGGATTT CCTAAATGCA 
CTGCAGTCTC CCATAGAGTA CCAACGCAAA GAAAGTAGCA CAGCAGTGCT GAGGCCCCAG 
GCTGGTAGGG TCGCTCAGCC CAACAGCAGA CCCTATTCTT CTGTTCCAGT AGGGTCTGAG 
AAGCCTTTGA AGGCTACAAG CCGTAACCCA TCTCTCCCAC CCCTGAAGAT ATCTACCTCC 
AACGGCAGTA CAGGGTATGA ACAGCCCGGA GACAAGTATG AACAAAGCAA GACTGATTTG 
GGAGGGTGCA ATGGGACATC TTCCATGGTG GTGATTAAAG ATTACTATGC AGTGAAGGAG 
GACGAGATCT GTGTGAATCA AGGAGAGGTG GTTCAGATCC TTGCTATCAA CCAGCAAAAC 
ATGTTCCTGG TGTACCAGCC TGCAAATGAC CACTCTCCAG CTGCTGAAGG ATGGATCCCT 
GGCAATATCT TGGCTCCCCT CACTAAACCC ACTACAGATA ATAGCGACGG AAGCATCAAG 
AAGTCATGTT CATGGCATAC CCTGCGCATG AGAAAGCGGG CGGAAAAGGA GAGCAGTGGC 
AAAAATGAAG CCAATGGGCC ACGTAAATCC AAGGATATTC TGGGGAACAA AGTCTCTGTT 
AAAGAGACAA ACAGCTCAGA GGAATCAGAG TGTGATGACC TTGACCCCAA TACTAGCATG 
GAGATAATCA ACCCCAATTT CATTCAGGAA GTGGCTCCAG AGTTCCTTGT GCTGTTGGTG 
GATATCACCT GCCTGCTTGG CGACACTGTG ATGCTGCAGT GCAAAGTCTG CGGCCGGCCC 
AAACCAACAA TAACCTGGAA AGGACCTGAT CAAAACATTC TTGACAATGA CAACAGCACG 
GCCACATACA CGGTGTCATC CTGTGACTCT GGAGAGCTCA TGCTGAAGAT CTGCAATGTG 
ATGCCTCAGG ACAGTGGCAT TTACACTTGT GTGGCAACTA ACGAGCATGG AACGGCATCA 
ACCTCAGCCA CAATCAAAGT GCAAGGTGTA CCGGCTGCCC CAAATCGCCC CATTGCTCAG 
GAGAGAAGCT GCACCTCTGT GATCCTTCGC TGGCTGCCTC CATCCAGTAC AGGCAATTGC 
ACCATTTCTG GCTACACAGT GGAGTACAGA GAAGAAGGCT CACAGGTCTG GCAGCAGTCA 
GTGGCCTCCA CACTGGACAC TTACCTTGTC ATTGAAGACC TGTCCCCAGG CTGCCAGTAT 
CAGTTCCGTG TGAGTGCCAG CAACCCCTGG GGGATCAGCT TGCCCAGTGA CCCATCTGAA 
TTCGTGAGGC TTCCAGAGTA TGATTCTGCT GCTGATGGTG CCACTATTTC GTGGAAGGAA 
AACTTTGATC TCGCTTATGC GGAGCTGCAT GAGATTGGGA GGGGACGATT CTCCATTGTA 
AAGAAATGCG TTCACAAAGC TACCCGGAAA GACGTGGCTG TAAAATTCAT CAGTAAAAAA 
ATGAAGAAGA AGGAGCAAGC AGCCCACGAG GCAGCTCTGT TACAGCACTT ACAGCATCCT 
CAGTACATCA CTATCCATGA TACCTATGAG TCTCCTACTT CTTACATCCT AGTCCTGGAG 
CTGATGGATG ATGGTCGTCT CCTAGATTAT CTAATGAACC ATGATGAACT TATGGAGGAA 
AAAGTAGCTT TCTACATAAG AGACACAATG GAAGCCTTGC AGTATCTTCA CAATTGCCGA 
GTGGCTCATT TAGATATAAA GCCAGAGAAT CTGCTCATCG ATTTAAGGAT CCCAGTTCCT 
CGTGTCAAGA TCATTGATTT GGAAGATGCA GTTCAAATCA CAGGCCATTA CCACGTCCAC 
CATCTCCTTG GAAACCCAGA GTTTGCAGCC CCTGAAGTTA TCCAGGGCCT CCCCGTGTCC 
CTGAGCACAG ACATCTGGAG CATCGGGGTC CTCACCTACG TCATGCTGAG CGGTGTCTCC 
CCGTTCCTGG ATGAAAGCAA AGAGGAGACG TGTATCAATG TGTGCAGAGT CGATTTCAGC 
TTCCCGCCCG AGTACTTCTC TGATGTGAGC CATGCTGCTA GGGATTTCAT CAACGTCATC 
CTCCAGGAAG ACTCCAGGAG GAGGCCGACA GCAGCCACTT GTTTACAGCA CCCATGGCTG 
CAGCCACACA ATGGCAGCTA CTCCAAAATC CCGCTGGATA CCTCCCGCTT GGCATCCTTC 
ATTGAGCGCC GCAGACACCA GTACGACGTG CACCCCGTCC CCGGTGTCAA GAGTTTCCTG 
ATGAGCAGGC TGAACCCCGG GACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KALRN ( XM_015289987.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145473.1 Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X12, mRNA.

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CloneID	OGa38682
Clone ID Related Accession (Same CDS sequence)	XM_015289987.1
Accession Version	XM_015289987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	kalirin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG AAGACACACC AGAGTGCCCT GCAGGTCCAG 
CAGAAAGCCG AAGTGCTGCT GCAGGCTGGG CATTATGATG CTGATGCCAT CAGAGAATGC 
GCTGAAAAGG TGGCCCTGCA CTGGCAGCAG CTGATGCTGA AGATGGAGGA CAGGCTCAAG 
CTGGTCAATG CCTCGGTGGC CTTCTACAAA ACCTCTGAGC AGGTGTGCAG TGTATTAGAG 
AGCCTGGAGC AAGAATACAG ACGTGATGAA GACTGGTGTG GAGGTCGAGA TAAACTTGGA 
CCAGCAGCTG AGATAGACCA CGTCATCCCT CTGATCAGCA AGCATCTGGA GCAGAAGGAG 
GCTTTTCTCA AGGCCTGCAC ACTGGCTCGA AGGAATGCTG AGGTATTCCT CAAGTACATC 
CACAGGAACA ATGTCAGCAT GCCCGGAGTC GCAAGCCATA CACGGGGACC TGAGCAGCAA 
GTGAAAGCCA TCCTGAGTGA GTTGCTGCAG AGGGAGAACC GGGTCCTTCA CTTCTGGACC 
CTGAAGAAGC GGAGGTTAGA TCAGTGCCAG CAGTACGTTG TATTTGAGCG CAGTGCCAAG 
CAGGCCCTGG ACTGGATCCA AGAGACAGGC GAATATTACC TCTCTACTCA CAACTCTACA 
GGGGAATCAG CAGAAGAAAC TCAGGAACTC CTGAAGGAAT ATGGGGAATT CAGAGTACCT 
GCGAAGCAAA CAAAGGAGAA AGTGAAGCTC CTCATCCAGC TGGCTGACAG CTTTGTAGAA 
AAAGGACATA TACACGCCAC TGAAATCAGG AAGTGGGTCA CCACCGTTGA CAAACGCTAC 
CGTGACTTCT CTCTCAGGAT GGGGAAATAC CGCTACTCCC TGGAGAAAGC CCTTGGAATC 
AATACAGAGG ATAACAAGGA CCTCGAACTA GATATTATTC CAGCAAGTCT TTCAGACCGG 
GAGGTAAAGC TGCGAGATGC AAATCACGAA GTCAATGAAG AAAAAAGAAA GTCTGCTAGA 
AAGAAAGAAT TCATTATGGC CGAGCTTCTT CAGACAGAGA AAGCTTATGT CAGGGACTTA 
CATGAATGTT TAGAGACTTA CCTTTGGGAA ATGACAAGTG GGGTGGAAGA AATACCCGCT 
GGGATCCTTA ATAAAGAACA CATCATCTTT GGCAATATTC AGGAGATATA TGACTTCCAT 
AACAACATTT TCCTGAAAGA ACTCGAGAAG TATGAGCAAC TGCCTGAGGA TGTGGGTCAC 
TGCTTTGTCA CCTGGGCAGA TAAATTTCAG ATGTATGTCA CCTACTGCAA AAACAAGCCT 
GACTCCAGCC AGCTCATCCT GGAACACGCA GGGACTTTCT TTGATGAAAT TCAGCAAAGG 
CATGGTCTGG CCAACTCTAT CTCTTCTTAT TTAATCAAGC CTGTCCAGAG GATCACAAAA 
TATCAACTCC TCCTGAAGGA ACTGCTTACC TGCTGTGAGG AAGGCAAAGG GGAACTCAAG 
GATGGTCTGG AAGTGATGCT CAGTGTCCCC AAGAAAGCCA ATGATGCAAT GCACGTCAGC 
ATGCTGGAAG GGTTTGATGA GAATCTAGAC GTCCAGGGAG AGCTGATTCT TCAGGATTCC 
TTCCAAGTGT GGGACCCCAA GTCTCTCATT CGGAAAGGCC GTGAGAGACA TTTGTTTCTC 
TTTGAAATCT CTTTGGTGTT TAGCAAAGAG ATCAAAGACT CTTCAGGACA CACAAAATAT 
GTTTACAAGA ACAAGTTACT GACCTCAGAA CTGGGAGTGA CTGAACATGT TGAAGGAGAT 
CCCTGTAAAT TTGCCTTGTG GTCTGGACGA ACACCATCCT CAGACAATAA AACAGTGCTG 
AAAGCATCGA GTATTGAAAC AAAACAGGAG TGGATCAAAA ATATCCGAGA AGTCATCCAA 
GAAAGAATTA TCCATCTGAA AGGAGCTCTG AAAGAGCCAA TTCAGCTCCC CAAGACACCA 
GCAAAGCAGC GAAACAACAG TAGAAGAGAT GGAGTAGATG ATGCAGACAG CCAAGGAGAT 
GGAAGCAGTC AACCTGACAC CATTTCCATT GCATCCAGGA CATCACAGAA TACAGTGGAC 
AGTGATAAGT TGTCTGGAGG GTGTGAACTA ACCGTGGTGC TCCAGGACTT CACGGCGGGC 
CACAGCAGCG AGCTGAGCAT TCAGGTGGGG CAGACAGTGG AGCTGCTGGA GAGGCCAAGT 
GAAAGGCCAG GCTGGTGCTT GGTGCGGACC ACGGAGCGGA GCCCACCTCA GGAAGGCCTG 
GTCCCCAGCA GCGCTCTCTG CATTTCCCAT TCCCGCAGCA GCGTGGAGAT GGATTGCTTC 
TTCCCTTCAG GAAAAGAGGC ATATTCAGTC TCTTCCAATG AAAACGGGGG CAAGTCCGAT 
TCAGTGGCCA ACTTACAGCC CCAGCCATCC CTCAACTCCA TCCAGAGCTC TCCCGGCCCC 
AAGCGCTCGA CCAACACGCT GAAGAAATGG CTGACGAGCC CCGTGCGCCG GCTGAACAGC 
GGGAAGGCCG AGAGCAACAT CAAGAAGCAA AAGAAGGTGC GAGATGGCAG GAAGAGCTTT 
GACCTCGGCT CGCCGAAGAC TGGAGATGAA ACCACTCCTC AAGGAGACAG CGCGGATGAG 
AAGAGCAAGA AGGGTTGGGG AGAAGATGAG CCAGATGAAG AGTCTCACAC ACCTCTTCCT 
CCTCCTATGA AGATTTTTGA CAATGATCCA ACCCAAGATG AGATGTCCTC TTCTTTGCTA 
GCTGCTCGGC AGAGCTCCTC CGATGTCCCA ACTGCTGCGG ATCTTGTCAG TGCAATAGAA 
CAGTTGGTCA AAAGTAAGCT GACCCTTGAA GGAGGATCTT ACCGAGGAAG CTTAAAAGAT 
GCCACAGGCT GCTTGAATGA AGGAATGGCG CCTTCAACTC CCCCAAGAAA CCTTGAAGAG 
GAACAAAAAG CCAAAGCAAT GAGAGGCAGG ATGTTTGTCT TAAATGAACT GGTCCAGACT 
GAAAAAGACT ACGTCAAAGA CTTGGGAACC GTTGTGGAGG GCTTCATGAA GAGAATAGAA 
GAAAAGGGAG TTTCCGAAGA TATGAAAGGG AAAGACAAGA TAGTGTTTGG CAATATTCAC 
CAGATCTATG ACTGGCACAA AGACTTTTTC CTGGCTGAAC TGGAAAAATG TCTTCAGGAG 
CCTGACCGTT TAGCACAGCT TTTTATTAAA CATGAGCGGC GATTACACAT GTATGTGGTG 
TACTGCCAAA ACAAGCCACG ATCTGAATAT ATAGTAGCTG AATACGAGGC ATATTTTGAG 
GAGGTTCAAC AGGAAATAAA CCAACGGCTG ACCATCAGTG ACTTTCTGAT CAAACCCATT 
CAGAGAATAA CTAAATATCA GCTTCTTCTC AAGGACTTCC TGAGGTACAG TGAAAAAGCT 
GGTCTGGAGT GCTCTGAAAT AGAGAAAGCA GTTGAGTTAA TGTGCCTTGT ACCAAAACGT 
TGCAATGATA TGATGAACCT GGGTCGCCTC CAAGGCTTTG AGGGTAAGCT GACAGCCCAG 
GGCAAGCTGC TGCAGCAGGA CACCTTCTAC GTGACAGAGC ATGACTCCGG AGTTCAGTCC 
CGACCAAAGG AACGTAGGGT GTTTCTCTTT GAGCAAATAG TTATCTTCAG CGAGCTGCTT 
AGGAAAGGGT CTTTAACACC AGGCTACATG TTCAAGAGAA GCATAAAGAT GAACTACCTT 
GTCATTGAAG AAAATGTGGA TGGTGATCCC TGCAAGTTTG CTCTAATGAA TCGGGAAACA 
TCTGAGAAAG TTGTTTTACA GGCAGCTAAT CCAGAAATTC AGCAGGCTTG GGTACAGGAC 
ATCAACCAGG TCCTGGACAC ACAAAGGGAT TTCCTAAATG CACTGCAGTC TCCCATAGAG 
TACCAACGCA AAGAAAGTAG CACAGCAGTG CTGAGGCCCC AGGCTGGTAG GGTCGCTCAG 
CCCAACAGCA GACCCTATTC TTCTGTTCCA GTAGGGTCTG AGAAGCCTTT GAAGGCTACA 
AGCCGTAACC CATCTCTCCC ACCCCTGAAG ATATCTACCT CCAACGGCAG TACAGGGTAT 
GAACAGCCCG GAGACAAGTA TGAACAAAGC AAGACTGATT TGGGAGGGTG CAATGGGACA 
TCTTCCATGG TGGTGATTAA AGATTACTAT GCAGTGAAGG AGGACGAGAT CTGTGTGAAT 
CAAGGAGAGG TGGTTCAGAT CCTTGCTATC AACCAGCAAA ACATGTTCCT GGTGTACCAG 
CCTGCAAATG ACCACTCTCC AGCTGCTGAA GGATGGATCC CTGGCAATAT CTTGGCTCCC 
CTCACTAAAC CCACTACAGA TAATAGCGAC GGAAGCATCA AGAAGTCATG TTCATGGCAT 
ACCCTGCGCA TGAGAAAGCG GGCGGAAAAG GAGAGCAGTG GCAAAAATGA AGCCAATGGG 
CCACGTAAAT CCAAGGATAT TCTGGGGAAC AAAGTCTCTG TTAAAATAAT CAACCCCAAT 
TTCATTCAGG AAGTGGCTCC AGAGTTCCTT GTGCTGTTGG TGGATATCAC CTGCCTGCTT 
GGCGACACTG TGATGCTGCA GTGCAAAGTC TGCGGCCGGC CCAAACCAAC AATAACCTGG 
AAAGGACCTG ATCAAAACAT TCTTGACAAT GACAACAGCA CGGCCACATA CACGGTGTCA 
TCCTGTGACT CTGGAGAGCT CATGCTGAAG ATCTGCAATG TGATGCCTCA GGACAGTGGC 
ATTTACACTT GTGTGGCAAC TAACGAGCAT GGAACGGCAT CAACCTCAGC CACAATCAAA 
GTGCAAGGTG TACCGGCTGC CCCAAATCGC CCCATTGCTC AGGAGAGAAG CTGCACCTCT 
GTGATCCTTC GCTGGCTGCC TCCATCCAGT ACAGGCAATT GCACCATTTC TGGCTACACA 
GTGGAGTACA GAGAAGAAGG CTCACAGGTC TGGCAGCAGT CAGTGGCCTC CACACTGGAC 
ACTTACCTTG TCATTGAAGA CCTGTCCCCA GGCTGCCAGT ATCAGTTCCG TGTGAGTGCC 
AGCAACCCCT GGGGGATCAG CTTGCCCAGT GACCCATCTG AATTCGTGAG GCTTCCAGAG 
TATGATTCTG CTGCTGATGG TGCCACTATT TCGTGGAAGG AAAACTTTGA TCTCGCTTAT 
GCGGAGCTGC ATGAGATTGG GAGGGGACGA TTCTCCATTG TAAAGAAATG CGTTCACAAA 
GCTACCCGGA AAGACGTGGC TGTAAAATTC ATCAGTAAAA AAATGAAGAA GAAGGAGCAA 
GCAGCCCACG AGGCAGCTCT GTTACAGCAC TTACAGCATC CTCAGTACAT CACTATCCAT 
GATACCTATG AGTCTCCTAC TTCTTACATC CTAGTCCTGG AGCTGATGGA TGATGGTCGT 
CTCCTAGATT ATCTAATGAA CCATGATGAA CTTATGGAGG AAAAAGTAGC TTTCTACATA 
AGAGACACAA TGGAAGCCTT GCAGTATCTT CACAATTGCC GAGTGGCTCA TTTAGATATA 
AAGCCAGAGA ATCTGCTCAT CGATTTAAGG ATCCCAGTTC CTCGTGTCAA GATCATTGAT 
TTGGAAGATG CAGTTCAAAT CACAGGCCAT TACCACGTCC ACCATCTCCT TGGAAACCCA 
GAGTTTGCAG CCCCTGAAGT TATCCAGGGC CTCCCCGTGT CCCTGAGCAC AGACATCTGG 
AGCATCGGGG TCCTCACCTA CGTCATGCTG AGCGGTGTCT CCCCGTTCCT GGATGAAAGC 
AAAGAGGAGA CGTGTATCAA TGTGTGCAGA GTCGATTTCA GCTTCCCGCC CGAGTACTTC 
TCTGATGTGA GCCATGCTGC TAGGGATTTC ATCAACGTCA TCCTCCAGGA AGACTCCAGG 
AGGAGGCCGA CAGCAGCCAC TTGTTTACAG CACCCATGGC TGCAGCCACA CAATGGCAGC 
TACTCCAAAA TCCCGCTGGA TACCTCCCGC TTGGCATCCT TCATTGAGCG CCGCAGACAC 
CAGTACGACG TGCACCCCGT CCCCGGTGTC AAGAGTTTCC TGATGAGCAG GCTGAACCCC 
GGGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145473.1
CDS	234..9062
Translation	MNPTEGAAEEGAVPDSEVDAFFRTGSFRNDGLKAADVLPILKEKVAFVSGGRDKRGGPVLTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLAKLVDPSQLTDEFEGSLDYNHDEWIELRVSLEEFFNSAIHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVIKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKELLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAVSNNKTPHNSSISHIESVLQQLDEAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDIDLATQVQELLEFLHEKQHELELNADQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIEKTHQSALQVQQKAEVLLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEIDHVIPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPGVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHNSTGESAEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKRYRDFSLRMGKYRYSLEKALGINTEDNKDLELDIIPASLSDREVKLRDANHEVNEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPAGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSSQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDSFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASSIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKQRNNSRRDGVDDADSQGDGSSQPDTISIASRTSQNTVDSDKLSGGCELTVVLQDFTAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPSGKEAYSVSSNENGGKSDSVANLQPQPSLNSIQSSPGPKRSTNTLKKWLTSPVRRLNSGKAESNIKKQKKVRDGRKSFDLGSPKTGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSSLLAARQSSSDVPTAADLVSAIEQLVKSKLTLEGGSYRGSLKDATGCLNEGMAPSTPPRNLEEEQKAKAMRGRMFVLNELVQTEKDYVKDLGTVVEGFMKRIEEKGVSEDMKGKDKIVFGNIHQIYDWHKDFFLAELEKCLQEPDRLAQLFIKHERRLHMYVVYCQNKPRSEYIVAEYEAYFEEVQQEINQRLTISDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSEIEKAVELMCLVPKRCNDMMNLGRLQGFEGKLTAQGKLLQQDTFYVTEHDSGVQSRPKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVIEENVDGDPCKFALMNRETSEKVVLQAANPEIQQAWVQDINQVLDTQRDFLNALQSPIEYQRKESSTAVLRPQAGRVAQPNSRPYSSVPVGSEKPLKATSRNPSLPPLKISTSNGSTGYEQPGDKYEQSKTDLGGCNGTSSMVVIKDYYAVKEDEICVNQGEVVQILAINQQNMFLVYQPANDHSPAAEGWIPGNILAPLTKPTTDNSDGSIKKSCSWHTLRMRKRAEKESSGKNEANGPRKSKDILGNKVSVKIINPNFIQEVAPEFLVLLVDITCLLGDTVMLQCKVCGRPKPTITWKGPDQNILDNDNSTATYTVSSCDSGELMLKICNVMPQDSGIYTCVATNEHGTASTSATIKVQGVPAAPNRPIAQERSCTSVILRWLPPSSTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCQYQFRVSASNPWGISLPSDPSEFVRLPEYDSAADGATISWKENFDLAYAELHEIGRGRFSIVKKCVHKATRKDVAVKFISKKMKKKEQAAHEAALLQHLQHPQYITIHDTYESPTSYILVLELMDDGRLLDYLMNHDELMEEKVAFYIRDTMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKIIDLEDAVQITGHYHVHHLLGNPEFAAPEVIQGLPVSLSTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPPEYFSDVSHAARDFINVILQEDSRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLASFIERRRHQYDVHPVPGVKSFLMSRLNPGT

Target ORF information:

RefSeq Version	XM_015289987.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus kalirin, RhoGEF kinase (KALRN), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289987.1

ORF Insert Sequence:

ATGAATCCCA CCGAAGGGGC GGCGGAGGAA GGCGCTGTCC CCGACTCGGA GGTGGATGCT 
TTCTTCCGAA CAGGCTCATT TCGAAATGAT GGCTTGAAAG CTGCTGATGT CCTTCCTATA 
CTAAAGGAGA AAGTGGCCTT CGTATCAGGT GGCCGTGATA AACGAGGTGG TCCTGTCTTG 
ACCTTCCCAG CCCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTTCG GAAACTTGTG 
ACTTATTTGG CCAGCGTGCC AAGCGAAGAT GTCTGTAAAC GAGGATTCAC TGTTATTATT 
GACATGCGGG GATCAAAATG GGATTTGATC AAGCCTCTCC TAAAGACCCT TCAAGAAGCC 
TTTCCTGCCG AGATTCATGT TGCCCTGATC ATAAAACCTG ATAATTTCTG GCAGAAGCAG 
AAGACAAACT TCGGCAGTTC CAAGTTCATC TTTGAGACAA GTATGGTGTC TGTTGAAGGC 
CTGGCAAAAC TTGTAGATCC GTCACAACTG ACAGATGAGT TTGAAGGATC CTTGGATTAC 
AACCACGATG AATGGATAGA GCTGCGTGTC TCCTTGGAAG AGTTTTTCAA CAGTGCCATC 
CATTTATTAT CAAGGCTGGA GGACCTCCAG GAAATGCTGG CTAGGAAGGA GTTTCCAGTT 
GACGTCGAGG GCTCCAGGAG GCTGATTGAT GAGCACACAC AGCTTAAGAA GAAAGTGATC 
AAAGCTCCTG TGGAAGAACT GGACCGTGAG GGGCAGAGGT TATTACAATG CATAAGGTGC 
AGTGATGGTT TTTCCGGTAG GAACTGCATT CCTGGAAGTG CGGATTTTCA AAGTCTTGTG 
CCAAAGATTA CCAGCCTTTT AGACAAGCTG CATTCTACCC GGCAACACTT ACATCAGATG 
TGGCATGTCC GCAAGTTGAA ACTAGACCAG TGCTTTCAAC TCCGGCTTTT TGAGCAAGAT 
GCTGAGAAGA TGTTTGACTG GATCAGCCAC AACAAAGAAT TGTTCCTGCA GAGTCACACG 
GAGATCGGCG TGAGCTACCA ACATGCCCTT GACCTGCAGA CGCAGCACAA TCACTTTGCC 
ATGAATTCGA TGAACGCCTA TGTCAACATC AATCGGATCA TGTCTGTTGC ATCCAGGCTG 
TCTGAGGCAG GCCATTACGC TTCCCAGCAA ATTAAACAAA TTTCTACACA GCTGGATCAA 
GAGTGGAAGA GTTTTGCTGC AGCTCTGGAT GAACGCAGCA CTATCTTAGC CATGTCTGCT 
GTCTTTCACC AGAAAGCTGA ACAGTTCCTT TCAGGTGTGG ATGCCTGGTG TAAAATGTGC 
AGTGAAGGAG GCCTCCCCTC AGAAATGCAA GACCTGGAGC TGGCCATCCA TCATCATCAG 
TCTCTGTATG AGCAAGTAAC ACAGGCCTAC ACAGAGGTAA GCCAGGATGG AAAAGCCTTG 
CTGGATGTCC TGCAGCGTCC CCTAAGCCCT GGGAATTCCG AATCCCTCAC TGCTACTGCA 
AACTACTCCA AGGCTGTGCA CCAGGTGCTG GACGTGGTAC ATGAGGTCCT ACATCACCAG 
CGGCGCCTGG AGAGCATCTG GCAGCACCGA AAGGTCCGGC TGCACCAAAG ACTGCAGCTC 
TGCGTTTTCC AGCAGGATGT TCAGCAGGTA CTGGACTGGA TTGAAAATCA TGGAGAAGCC 
TTTCTGAGCA AACACACAGG AGTGGGGAAG TCTCTGCACA GAGCACGTGC GCTGCAGAAA 
AGACACGATG ACTTTGAAGA GGTAGCACAG AATACGTACA CCAATGCAGA CAAGCTTTTA 
GAGGCTGCAG AGCAGTTGGC TCAGACTGGG GAATGTGACC CTGAAGAGAT CTATAAAGCA 
GCCCGGCATC TGGAAGTACG GATTCAGGAC TTCGTCCGGA GGGTGGAACA GAGGAAGCTT 
CTCTTGGACA TGTCAGTCTC CTTCCATACC CACACCAAAG AGCTGTGGAC ATGGATGGAA 
GATCTGCAGA AGGAGCTCTT AGAGGATGTC TGTGCTGACT CTGTGGATGC GGTTCAGGAG 
CTTATCAAGC AGTTTCAGCA GCAGCAAACA GCCACCTTGG ATGCTACCCT CAATGTTATT 
AAGGAAGGGG AAGATCTAAT CCAGCAGCTC AGGGACTCCG CCGTTTCCAA CAATAAGACC 
CCACACAACA GCTCCATCAG CCACATTGAA TCTGTACTTC AGCAGCTGGA CGAGGCCCAG 
GTACAGATGG AAGAGCTCTT CCATGAGAGG AAGATTAAGC TAGATATCTT TCTTCAGCTC 
CGGATCTTTG AGCAGTACAC CATTGAGGTT ACAGCAGAGT TAGATGCCTG GAATGAGGAT 
CTGCTGAGAC AAATGAATGA TTTCAATACC GAGGACCTTA CTCTGGCTGA GCAACGTTTG 
CAGCGTCACA CCGAGAGGAA GTTGGCCATG AACAACATGA CTTTTGAAGT CATCCAGCAA 
GGGCAAGATT TACACCAATA CATCATGGAG GTCCAGGCTT CAGGCATCGA GCTGATCTGT 
GAAAAGGACA TTGACCTTGC AACACAGGTT CAAGAGCTGC TGGAATTCCT TCATGAGAAG 
CAGCATGAGC TGGAGCTTAA TGCTGACCAA ACTCACAAGA GGTTAGAGCA GTGCCTACAG 
CTCCGGCACC TACAGGCTGA GGTCAAGCAG GTGCTTGGTT GGATCCGGAA TGGTGAATCA 
ATGCTGAATG CAAGCCTCGT CAATGCAAGC TCCCTCTCTG AGGCTGAGCA GCTCCAGCGA 
GAGCATGAGC AATTTCAGCT GGCCATAGAG AAGACACACC AGAGTGCCCT GCAGGTCCAG 
CAGAAAGCCG AAGTGCTGCT GCAGGCTGGG CATTATGATG CTGATGCCAT CAGAGAATGC 
GCTGAAAAGG TGGCCCTGCA CTGGCAGCAG CTGATGCTGA AGATGGAGGA CAGGCTCAAG 
CTGGTCAATG CCTCGGTGGC CTTCTACAAA ACCTCTGAGC AGGTGTGCAG TGTATTAGAG 
AGCCTGGAGC AAGAATACAG ACGTGATGAA GACTGGTGTG GAGGTCGAGA TAAACTTGGA 
CCAGCAGCTG AGATAGACCA CGTCATCCCT CTGATCAGCA AGCATCTGGA GCAGAAGGAG 
GCTTTTCTCA AGGCCTGCAC ACTGGCTCGA AGGAATGCTG AGGTATTCCT CAAGTACATC 
CACAGGAACA ATGTCAGCAT GCCCGGAGTC GCAAGCCATA CACGGGGACC TGAGCAGCAA 
GTGAAAGCCA TCCTGAGTGA GTTGCTGCAG AGGGAGAACC GGGTCCTTCA CTTCTGGACC 
CTGAAGAAGC GGAGGTTAGA TCAGTGCCAG CAGTACGTTG TATTTGAGCG CAGTGCCAAG 
CAGGCCCTGG ACTGGATCCA AGAGACAGGC GAATATTACC TCTCTACTCA CAACTCTACA 
GGGGAATCAG CAGAAGAAAC TCAGGAACTC CTGAAGGAAT ATGGGGAATT CAGAGTACCT 
GCGAAGCAAA CAAAGGAGAA AGTGAAGCTC CTCATCCAGC TGGCTGACAG CTTTGTAGAA 
AAAGGACATA TACACGCCAC TGAAATCAGG AAGTGGGTCA CCACCGTTGA CAAACGCTAC 
CGTGACTTCT CTCTCAGGAT GGGGAAATAC CGCTACTCCC TGGAGAAAGC CCTTGGAATC 
AATACAGAGG ATAACAAGGA CCTCGAACTA GATATTATTC CAGCAAGTCT TTCAGACCGG 
GAGGTAAAGC TGCGAGATGC AAATCACGAA GTCAATGAAG AAAAAAGAAA GTCTGCTAGA 
AAGAAAGAAT TCATTATGGC CGAGCTTCTT CAGACAGAGA AAGCTTATGT CAGGGACTTA 
CATGAATGTT TAGAGACTTA CCTTTGGGAA ATGACAAGTG GGGTGGAAGA AATACCCGCT 
GGGATCCTTA ATAAAGAACA CATCATCTTT GGCAATATTC AGGAGATATA TGACTTCCAT 
AACAACATTT TCCTGAAAGA ACTCGAGAAG TATGAGCAAC TGCCTGAGGA TGTGGGTCAC 
TGCTTTGTCA CCTGGGCAGA TAAATTTCAG ATGTATGTCA CCTACTGCAA AAACAAGCCT 
GACTCCAGCC AGCTCATCCT GGAACACGCA GGGACTTTCT TTGATGAAAT TCAGCAAAGG 
CATGGTCTGG CCAACTCTAT CTCTTCTTAT TTAATCAAGC CTGTCCAGAG GATCACAAAA 
TATCAACTCC TCCTGAAGGA ACTGCTTACC TGCTGTGAGG AAGGCAAAGG GGAACTCAAG 
GATGGTCTGG AAGTGATGCT CAGTGTCCCC AAGAAAGCCA ATGATGCAAT GCACGTCAGC 
ATGCTGGAAG GGTTTGATGA GAATCTAGAC GTCCAGGGAG AGCTGATTCT TCAGGATTCC 
TTCCAAGTGT GGGACCCCAA GTCTCTCATT CGGAAAGGCC GTGAGAGACA TTTGTTTCTC 
TTTGAAATCT CTTTGGTGTT TAGCAAAGAG ATCAAAGACT CTTCAGGACA CACAAAATAT 
GTTTACAAGA ACAAGTTACT GACCTCAGAA CTGGGAGTGA CTGAACATGT TGAAGGAGAT 
CCCTGTAAAT TTGCCTTGTG GTCTGGACGA ACACCATCCT CAGACAATAA AACAGTGCTG 
AAAGCATCGA GTATTGAAAC AAAACAGGAG TGGATCAAAA ATATCCGAGA AGTCATCCAA 
GAAAGAATTA TCCATCTGAA AGGAGCTCTG AAAGAGCCAA TTCAGCTCCC CAAGACACCA 
GCAAAGCAGC GAAACAACAG TAGAAGAGAT GGAGTAGATG ATGCAGACAG CCAAGGAGAT 
GGAAGCAGTC AACCTGACAC CATTTCCATT GCATCCAGGA CATCACAGAA TACAGTGGAC 
AGTGATAAGT TGTCTGGAGG GTGTGAACTA ACCGTGGTGC TCCAGGACTT CACGGCGGGC 
CACAGCAGCG AGCTGAGCAT TCAGGTGGGG CAGACAGTGG AGCTGCTGGA GAGGCCAAGT 
GAAAGGCCAG GCTGGTGCTT GGTGCGGACC ACGGAGCGGA GCCCACCTCA GGAAGGCCTG 
GTCCCCAGCA GCGCTCTCTG CATTTCCCAT TCCCGCAGCA GCGTGGAGAT GGATTGCTTC 
TTCCCTTCAG GAAAAGAGGC ATATTCAGTC TCTTCCAATG AAAACGGGGG CAAGTCCGAT 
TCAGTGGCCA ACTTACAGCC CCAGCCATCC CTCAACTCCA TCCAGAGCTC TCCCGGCCCC 
AAGCGCTCGA CCAACACGCT GAAGAAATGG CTGACGAGCC CCGTGCGCCG GCTGAACAGC 
GGGAAGGCCG AGAGCAACAT CAAGAAGCAA AAGAAGGTGC GAGATGGCAG GAAGAGCTTT 
GACCTCGGCT CGCCGAAGAC TGGAGATGAA ACCACTCCTC AAGGAGACAG CGCGGATGAG 
AAGAGCAAGA AGGGTTGGGG AGAAGATGAG CCAGATGAAG AGTCTCACAC ACCTCTTCCT 
CCTCCTATGA AGATTTTTGA CAATGATCCA ACCCAAGATG AGATGTCCTC TTCTTTGCTA 
GCTGCTCGGC AGAGCTCCTC CGATGTCCCA ACTGCTGCGG ATCTTGTCAG TGCAATAGAA 
CAGTTGGTCA AAAGTAAGCT GACCCTTGAA GGAGGATCTT ACCGAGGAAG CTTAAAAGAT 
GCCACAGGCT GCTTGAATGA AGGAATGGCG CCTTCAACTC CCCCAAGAAA CCTTGAAGAG 
GAACAAAAAG CCAAAGCAAT GAGAGGCAGG ATGTTTGTCT TAAATGAACT GGTCCAGACT 
GAAAAAGACT ACGTCAAAGA CTTGGGAACC GTTGTGGAGG GCTTCATGAA GAGAATAGAA 
GAAAAGGGAG TTTCCGAAGA TATGAAAGGG AAAGACAAGA TAGTGTTTGG CAATATTCAC 
CAGATCTATG ACTGGCACAA AGACTTTTTC CTGGCTGAAC TGGAAAAATG TCTTCAGGAG 
CCTGACCGTT TAGCACAGCT TTTTATTAAA CATGAGCGGC GATTACACAT GTATGTGGTG 
TACTGCCAAA ACAAGCCACG ATCTGAATAT ATAGTAGCTG AATACGAGGC ATATTTTGAG 
GAGGTTCAAC AGGAAATAAA CCAACGGCTG ACCATCAGTG ACTTTCTGAT CAAACCCATT 
CAGAGAATAA CTAAATATCA GCTTCTTCTC AAGGACTTCC TGAGGTACAG TGAAAAAGCT 
GGTCTGGAGT GCTCTGAAAT AGAGAAAGCA GTTGAGTTAA TGTGCCTTGT ACCAAAACGT 
TGCAATGATA TGATGAACCT GGGTCGCCTC CAAGGCTTTG AGGGTAAGCT GACAGCCCAG 
GGCAAGCTGC TGCAGCAGGA CACCTTCTAC GTGACAGAGC ATGACTCCGG AGTTCAGTCC 
CGACCAAAGG AACGTAGGGT GTTTCTCTTT GAGCAAATAG TTATCTTCAG CGAGCTGCTT 
AGGAAAGGGT CTTTAACACC AGGCTACATG TTCAAGAGAA GCATAAAGAT GAACTACCTT 
GTCATTGAAG AAAATGTGGA TGGTGATCCC TGCAAGTTTG CTCTAATGAA TCGGGAAACA 
TCTGAGAAAG TTGTTTTACA GGCAGCTAAT CCAGAAATTC AGCAGGCTTG GGTACAGGAC 
ATCAACCAGG TCCTGGACAC ACAAAGGGAT TTCCTAAATG CACTGCAGTC TCCCATAGAG 
TACCAACGCA AAGAAAGTAG CACAGCAGTG CTGAGGCCCC AGGCTGGTAG GGTCGCTCAG 
CCCAACAGCA GACCCTATTC TTCTGTTCCA GTAGGGTCTG AGAAGCCTTT GAAGGCTACA 
AGCCGTAACC CATCTCTCCC ACCCCTGAAG ATATCTACCT CCAACGGCAG TACAGGGTAT 
GAACAGCCCG GAGACAAGTA TGAACAAAGC AAGACTGATT TGGGAGGGTG CAATGGGACA 
TCTTCCATGG TGGTGATTAA AGATTACTAT GCAGTGAAGG AGGACGAGAT CTGTGTGAAT 
CAAGGAGAGG TGGTTCAGAT CCTTGCTATC AACCAGCAAA ACATGTTCCT GGTGTACCAG 
CCTGCAAATG ACCACTCTCC AGCTGCTGAA GGATGGATCC CTGGCAATAT CTTGGCTCCC 
CTCACTAAAC CCACTACAGA TAATAGCGAC GGAAGCATCA AGAAGTCATG TTCATGGCAT 
ACCCTGCGCA TGAGAAAGCG GGCGGAAAAG GAGAGCAGTG GCAAAAATGA AGCCAATGGG 
CCACGTAAAT CCAAGGATAT TCTGGGGAAC AAAGTCTCTG TTAAAATAAT CAACCCCAAT 
TTCATTCAGG AAGTGGCTCC AGAGTTCCTT GTGCTGTTGG TGGATATCAC CTGCCTGCTT 
GGCGACACTG TGATGCTGCA GTGCAAAGTC TGCGGCCGGC CCAAACCAAC AATAACCTGG 
AAAGGACCTG ATCAAAACAT TCTTGACAAT GACAACAGCA CGGCCACATA CACGGTGTCA 
TCCTGTGACT CTGGAGAGCT CATGCTGAAG ATCTGCAATG TGATGCCTCA GGACAGTGGC 
ATTTACACTT GTGTGGCAAC TAACGAGCAT GGAACGGCAT CAACCTCAGC CACAATCAAA 
GTGCAAGGTG TACCGGCTGC CCCAAATCGC CCCATTGCTC AGGAGAGAAG CTGCACCTCT 
GTGATCCTTC GCTGGCTGCC TCCATCCAGT ACAGGCAATT GCACCATTTC TGGCTACACA 
GTGGAGTACA GAGAAGAAGG CTCACAGGTC TGGCAGCAGT CAGTGGCCTC CACACTGGAC 
ACTTACCTTG TCATTGAAGA CCTGTCCCCA GGCTGCCAGT ATCAGTTCCG TGTGAGTGCC 
AGCAACCCCT GGGGGATCAG CTTGCCCAGT GACCCATCTG AATTCGTGAG GCTTCCAGAG 
TATGATTCTG CTGCTGATGG TGCCACTATT TCGTGGAAGG AAAACTTTGA TCTCGCTTAT 
GCGGAGCTGC ATGAGATTGG GAGGGGACGA TTCTCCATTG TAAAGAAATG CGTTCACAAA 
GCTACCCGGA AAGACGTGGC TGTAAAATTC ATCAGTAAAA AAATGAAGAA GAAGGAGCAA 
GCAGCCCACG AGGCAGCTCT GTTACAGCAC TTACAGCATC CTCAGTACAT CACTATCCAT 
GATACCTATG AGTCTCCTAC TTCTTACATC CTAGTCCTGG AGCTGATGGA TGATGGTCGT 
CTCCTAGATT ATCTAATGAA CCATGATGAA CTTATGGAGG AAAAAGTAGC TTTCTACATA 
AGAGACACAA TGGAAGCCTT GCAGTATCTT CACAATTGCC GAGTGGCTCA TTTAGATATA 
AAGCCAGAGA ATCTGCTCAT CGATTTAAGG ATCCCAGTTC CTCGTGTCAA GATCATTGAT 
TTGGAAGATG CAGTTCAAAT CACAGGCCAT TACCACGTCC ACCATCTCCT TGGAAACCCA 
GAGTTTGCAG CCCCTGAAGT TATCCAGGGC CTCCCCGTGT CCCTGAGCAC AGACATCTGG 
AGCATCGGGG TCCTCACCTA CGTCATGCTG AGCGGTGTCT CCCCGTTCCT GGATGAAAGC 
AAAGAGGAGA CGTGTATCAA TGTGTGCAGA GTCGATTTCA GCTTCCCGCC CGAGTACTTC 
TCTGATGTGA GCCATGCTGC TAGGGATTTC ATCAACGTCA TCCTCCAGGA AGACTCCAGG 
AGGAGGCCGA CAGCAGCCAC TTGTTTACAG CACCCATGGC TGCAGCCACA CAATGGCAGC 
TACTCCAAAA TCCCGCTGGA TACCTCCCGC TTGGCATCCT TCATTGAGCG CCGCAGACAC 
CAGTACGACG TGCACCCCGT CCCCGGTGTC AAGAGTTTCC TGATGAGCAG GCTGAACCCC 
GGGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	KALRN
Entrez Gene ID	424253
Full Name	kalirin, RhoGEF kinase
General protein information	Preferred Names kalirin, RhoGEF kinase Names kalirin
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Homo sapiens (human)	KALRN	NP_001019831.2
	KALRN	XP_002802737.1
Gallus gallus (chicken)	KALRN	XP_004942990.1
Mus musculus (house mouse)	Kalrn	NP_796331.2
Rattus norvegicus (Norway rat)	Kalrn	NP_114451.2
Bos taurus (cattle)	KALRN	NP_001178293.1
Canis lupus familiaris (dog)	KALRN	XP_535768.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

KALRN cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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