gRNAs
The following TRPM8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRPM8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OGa38186 | |
| Clone ID Related Accession (Same CDS sequence) | XM_025152160.1 , XM_015289172.1 | |
| Accession Version | XM_025152160.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3315bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-16 | |
| Organism | Gallus gallus(chicken) | |
| Product | transient receptor potential cation channel subfamily M member 8 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCTGTTCC AGGTTTCTAC AGGCACCATG AGGCACCGAA GAAATGGCAA TTTTGAGAGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_025007928.1 |
| CDS | 3362..6676 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_025152160.1 |
1 | ATGCTGTTCC AGGTTTCTAC AGGCACCATG AGGCACCGAA GAAATGGCAA TTTTGAGAGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa38186 | |
| Clone ID Related Accession (Same CDS sequence) | XM_025152160.1 , XM_015289172.1 | |
| Accession Version | XM_015289172.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3315bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-01-03 | |
| Organism | Gallus gallus(chicken) | |
| Product | transient receptor potential cation channel subfamily M member 8 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455901.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCTGTTCC AGGTTTCTAC AGGCACCATG AGGCACCGAA GAAATGGCAA TTTTGAGAGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015144658.1 |
| CDS | 3780..7094 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015289172.1 |
1 | ATGCTGTTCC AGGTTTCTAC AGGCACCATG AGGCACCGAA GAAATGGCAA TTTTGAGAGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa25468 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001007082.1 | |
| Accession Version | NM_001007082.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-20 | |
| Organism | Gallus gallus(chicken) | |
| Product | transient receptor potential cation channel subfamily M member 8 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY700573.1. On Nov 22, 2004 this sequence version replaced XM_421881.1. ##Evidence-Data-START## Transcript exon combination :: AY700573.1 [ECO:0000332] ##Evidence-Data-END## | |
1 | ATGAGGCACC AAAGAAATGG CAATTTTGAG AGTTCCAGAC TCCTCAATTC CAGCATATCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001007083.1 |
| CDS | 1..3288 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001007082.1 |
1 | ATGAGGCACC AAAGAAATGG CAATTTTGAG AGTTCCAGAC TCCTCAATTC CAGCATATCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Two different avian cold-sensitive sensory neurons: Transient receptor potential melastatin 8 (TRPM8)-dependent and -independent activation mechanisms. |
The super-cooling agent icilin reveals a mechanism of coincidence detection by a temperature-sensitive TRP channel. |
