P4HA1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	P4HA1
Entrez Gene ID	423704
Full Name	prolyl 4-hydroxylase subunit alpha 1
General protein information	Preferred Names prolyl 4-hydroxylase subunit alpha 1 Names prolyl 4-hydroxylase subunit alpha-1 4-PH alpha-1 procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1 prolyl 4-hydroxylase, alpha polypeptide I
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015288205.1	XP_015143691.1	prolyl 4-hydroxylase subunit alpha-1 isoform X1
XM_025151565.1	XP_025007333.1	prolyl 4-hydroxylase subunit alpha-1 isoform X1
NM_001198737.1	NP_001185666.1	prolyl 4-hydroxylase subunit alpha-1 isoform 3 precursor
NM_001198738.1	NP_001185667.1	prolyl 4-hydroxylase subunit alpha-1 isoform 4 precursor
XM_025151564.1	XP_025007332.1	prolyl 4-hydroxylase subunit alpha-1 isoform X1
NM_001198735.1	NP_001185664.1	prolyl 4-hydroxylase subunit alpha-1 isoform 1 precursor
NM_001198736.1	NP_001185665.1	prolyl 4-hydroxylase subunit alpha-1 isoform 2 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	P4HA1	XP_005619064.1
Bos taurus (cattle)	P4HA1	NP_001069238.1
Danio rerio (zebrafish)	p4ha1a	NP_001038463.1
Drosophila melanogaster (fruit fly)	PH4alphaEFB	NP_733371.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000971	XP_309164.4
Rattus norvegicus (Norway rat)	P4ha1	NP_742059.2
Caenorhabditis elegans (roundworm)	dpy-18	NP_499464.1
Xenopus tropicalis (tropical clawed frog)	LOC100490484	XP_002936004.1
	P4HA1	XP_001103971.2
Xenopus tropicalis (tropical clawed frog)	p4ha1	XP_004915844.1
Homo sapiens (human)	P4HA1	NP_001136067.1
Mus musculus (house mouse)	P4ha1	NP_035160.1
Danio rerio (zebrafish)	LOC556879	XP_005156147.1
Caenorhabditis elegans (roundworm)	phy-2	NP_502317.1
Pan troglodytes (chimpanzee)	P4HA1	XP_001140652.1
Gallus gallus (chicken)	P4HA1	NP_001185667.1

Gene Ontology
Bibliography

Related articles in PubMed

Cell density regulates prolyl 4-hydroxylase activity independent of mRNA levels.
Lee EH, Kao WW, Schwarz RI
Matrix biology : journal of the International Society for Matrix Biology19(8)779-82(2001 Jan)

Prolyl 4-hydroxylase: molecular cloning and the primary structure of the alpha subunit from chicken embryo.
Bassuk JA, Kao WW, Herzer P, Kedersha NL, Seyer J, DeMartino JA, Daugherty BL, Mark GE 3rd, Berg RA
Proceedings of the National Academy of Sciences of the United States of America86(19)7382-6(1989 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following P4HA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the P4HA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa00008	NM_001198735.1 Latest version!	Gallus gallus prolyl 4-hydroxylase subunit alpha 1 (P4HA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OGa00022	NM_001198738.1 Latest version!	Gallus gallus prolyl 4-hydroxylase subunit alpha 1 (P4HA1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OGa00064	NM_001198736.1 Latest version!	Gallus gallus prolyl 4-hydroxylase subunit alpha 1 (P4HA1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OGa00084	NM_001198737.1 Latest version!	Gallus gallus prolyl 4-hydroxylase subunit alpha 1 (P4HA1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OGa00008	XM_025151565.1 Latest version!	Gallus gallus prolyl 4-hydroxylase subunit alpha 1 (P4HA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OGa00008	XM_025151564.1 Latest version!	Gallus gallus prolyl 4-hydroxylase subunit alpha 1 (P4HA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OGa00084	XM_015288205.1 Latest version!	Gallus gallus prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

P4HA1 ( NM_001198735.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001185664.1 Gallus gallus prolyl 4-hydroxylase subunit alpha 1 (P4HA1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OGa00008
Clone ID Related Accession (Same CDS sequence)	XM_025151565.1 , XM_025151564.1 , NM_001198735.1
Accession Version	NM_001198735.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Gallus gallus(chicken)
Product	prolyl 4-hydroxylase subunit alpha-1 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000336.1. On Nov 14, 2010 this sequence version replaced XM_421583.2. Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: HAEK01030624.1, ERR2368565.4434.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2201357, SAMEA2201358 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCATCCA ACAAGATGTG GTACACAGTT GCCCTTGGAT TTCTGCTGCC TTTTTCTTAC 
GCCCATACGG ACTTCTTCAC CTCAATTGGC CATATGACAG ACTTAATTAA TACAGAGAAA 
GATCTGGTGA TATCACTGAA AGATTACATC AAGGCAGAAG AAAGCAAGCT GGAACAGATC 
AAAAAATGGG CAGAGAAATT AGATAAGCTG ACAGATACTG CTACGAAAGA CCCTGAGGGG 
TTTTTGGGAC ATCCTGTAAA TGCATTCAAG CTGATGAAAC GGCTTAACAC GGAGTGGGGC 
GAGCTGGAGA GCCTGGTTCT GAAGGACATG TCAGATGGCT TCATCTCCAA CATGACGATC 
CAGCGGCAGT TTTTTCCAAA TGATGAAGAT CAGACTGGCG CAGCAAAAGC TCTCTTGCGG 
CTTCAAGACA CGTATAATTT GGATACAGAC ACCCTCTCAA GAGGGAATCT TCCAGGTGTA 
AAGCACAAAT CCTTTTTAAC AGCTGAAGAT TGCTTTGAGC TGGGAAAGAT TGCATACACG 
GAGGCCGATT ACTACCACAC TGAGCTCTGG ATGGAGCAAG CTCTGAAGCA GCTGGATGAG 
GGAGAAGTGT CTTCTGCAGA TAAAGTCTAT ATTCTGGACT ACCTGAGTTA TGCTGTGTAT 
CAGCAGGGGG ATCTGAGCAA AGCAATGATG CTCACCAAGC GCCTTCTCGA ACTGGATCCT 
GAGCATCAAA GAGCAAATGG AAACATGAAG TATTTTGAAT ATATCATGGC CAAAGAAAAA 
GAAGCCAATA AGTCCAGTAC AGATGCAGAA GACCAGACAG AGAAGGAAAC TGAGGTTAAG 
AAAAAGGATT ACCTGCCTGA GAGAAGGAAG TACGAAATGC TGTGCCGTGG GGAGGGTCTC 
AAAATGACTC CTCGGAGACA AAAAAGACTG TTCTGCCGCT ACTACGATGG AAACAGGAAT 
CCAAGATACA TTCTGGGCCC TGTCAAGCAG GAAGATGAGT GGGACAAGCC TCGAATTGTT 
CGCTTTCTTG ATATCATTTC CGATGAAGAG ATTGAAACTG TGAAAGAACT AGCAAAGCCA 
AGGCTGAGCC GTGCTACTGT TCATGACCCC GAGACTGGGA AACTGACCAC AGCACATTAC 
AGAGTCTCTA AGAGTGCTTG GTTGTCTGGA TATGAAAGCC CTGTGGTATC TCGCATTAAC 
ACCAGGATAC AGGACCTAAC AGGGCTCGAT GTCTCCACAG CAGAGGAACT TCAGGTAGCC 
AACTACGGCG TAGGAGGCCA GTATGAGCCT CATTTTGATT TCGGAAGGAA AGACGAGCCA 
GATGCTTTTA AAGAATTGGG AACAGGCAAC AGAATTGCTA CTTGGTTGTT TTATATGAGC 
GACGTGTCAG CAGGGGGAGC TACCGTCTTT CCTGAAGTAG GAGCAAGCGT TTGGCCTAAA 
AAAGGAACAG CTGTATTCTG GTACAATCTC TTCCCAAGTG GAGAAGGAGA TTACAGCACA 
CGGCATGCAG CCTGCCCAGT ACTAGTTGGA AACAAATGGG TGTCCAATAA ATGGCTCCAC 
GAGCGGGGAC AGGAATTCCG AAGGCCGTGC ACGTTATCAG AGTTGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185664.1
CDS	106..1716
Translation	MASNKMWYTVALGFLLPFSYAHTDFFTSIGHMTDLINTEKDLVISLKDYIKAEESKLEQIKKWAEKLDKLTDTATKDPEGFLGHPVNAFKLMKRLNTEWGELESLVLKDMSDGFISNMTIQRQFFPNDEDQTGAAKALLRLQDTYNLDTDTLSRGNLPGVKHKSFLTAEDCFELGKIAYTEADYYHTELWMEQALKQLDEGEVSSADKVYILDYLSYAVYQQGDLSKAMMLTKRLLELDPEHQRANGNMKYFEYIMAKEKEANKSSTDAEDQTEKETEVKKKDYLPERRKYEMLCRGEGLKMTPRRQKRLFCRYYDGNRNPRYILGPVKQEDEWDKPRIVRFLDIISDEEIETVKELAKPRLSRATVHDPETGKLTTAHYRVSKSAWLSGYESPVVSRINTRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFGRKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFPSGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE

Target ORF information:

RefSeq Version	NM_001198735.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus prolyl 4-hydroxylase subunit alpha 1 (P4HA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198735.1

ORF Insert Sequence:

ATGGCATCCA ACAAGATGTG GTACACAGTT GCCCTTGGAT TTCTGCTGCC TTTTTCTTAC 
GCCCATACGG ACTTCTTCAC CTCAATTGGC CATATGACAG ACTTAATTAA TACAGAGAAA 
GATCTGGTGA TATCACTGAA AGATTACATC AAGGCAGAAG AAAGCAAGCT GGAACAGATC 
AAAAAATGGG CAGAGAAATT AGATAAGCTG ACAGATACTG CTACGAAAGA CCCTGAGGGG 
TTTTTGGGAC ATCCTGTAAA TGCATTCAAG CTGATGAAAC GGCTTAACAC GGAGTGGGGC 
GAGCTGGAGA GCCTGGTTCT GAAGGACATG TCAGATGGCT TCATCTCCAA CATGACGATC 
CAGCGGCAGT TTTTTCCAAA TGATGAAGAT CAGACTGGCG CAGCAAAAGC TCTCTTGCGG 
CTTCAAGACA CGTATAATTT GGATACAGAC ACCCTCTCAA GAGGGAATCT TCCAGGTGTA 
AAGCACAAAT CCTTTTTAAC AGCTGAAGAT TGCTTTGAGC TGGGAAAGAT TGCATACACG 
GAGGCCGATT ACTACCACAC TGAGCTCTGG ATGGAGCAAG CTCTGAAGCA GCTGGATGAG 
GGAGAAGTGT CTTCTGCAGA TAAAGTCTAT ATTCTGGACT ACCTGAGTTA TGCTGTGTAT 
CAGCAGGGGG ATCTGAGCAA AGCAATGATG CTCACCAAGC GCCTTCTCGA ACTGGATCCT 
GAGCATCAAA GAGCAAATGG AAACATGAAG TATTTTGAAT ATATCATGGC CAAAGAAAAA 
GAAGCCAATA AGTCCAGTAC AGATGCAGAA GACCAGACAG AGAAGGAAAC TGAGGTTAAG 
AAAAAGGATT ACCTGCCTGA GAGAAGGAAG TACGAAATGC TGTGCCGTGG GGAGGGTCTC 
AAAATGACTC CTCGGAGACA AAAAAGACTG TTCTGCCGCT ACTACGATGG AAACAGGAAT 
CCAAGATACA TTCTGGGCCC TGTCAAGCAG GAAGATGAGT GGGACAAGCC TCGAATTGTT 
CGCTTTCTTG ATATCATTTC CGATGAAGAG ATTGAAACTG TGAAAGAACT AGCAAAGCCA 
AGGCTGAGCC GTGCTACTGT TCATGACCCC GAGACTGGGA AACTGACCAC AGCACATTAC 
AGAGTCTCTA AGAGTGCTTG GTTGTCTGGA TATGAAAGCC CTGTGGTATC TCGCATTAAC 
ACCAGGATAC AGGACCTAAC AGGGCTCGAT GTCTCCACAG CAGAGGAACT TCAGGTAGCC 
AACTACGGCG TAGGAGGCCA GTATGAGCCT CATTTTGATT TCGGAAGGAA AGACGAGCCA 
GATGCTTTTA AAGAATTGGG AACAGGCAAC AGAATTGCTA CTTGGTTGTT TTATATGAGC 
GACGTGTCAG CAGGGGGAGC TACCGTCTTT CCTGAAGTAG GAGCAAGCGT TTGGCCTAAA 
AAAGGAACAG CTGTATTCTG GTACAATCTC TTCCCAAGTG GAGAAGGAGA TTACAGCACA 
CGGCATGCAG CCTGCCCAGT ACTAGTTGGA AACAAATGGG TGTCCAATAA ATGGCTCCAC 
GAGCGGGGAC AGGAATTCCG AAGGCCGTGC ACGTTATCAG AGTTGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P4HA1 ( NM_001198738.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001185667.1 Gallus gallus prolyl 4-hydroxylase subunit alpha 1 (P4HA1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OGa00022
Clone ID Related Accession (Same CDS sequence)	NM_001198738.1
Accession Version	NM_001198738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Gallus gallus(chicken)
Product	prolyl 4-hydroxylase subunit alpha-1 isoform 4 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000336.1. Transcript Variant: This variant (4) contains two alternate in-frame exons, compared to variant 1. It encodes isoform 4, which differs in sequence but is the same length as isoform 1. Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: HAEK01007518.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2201357, SAMEA2201358 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCATCCA ACAAGATGTG GTACACAGTT GCCCTTGGAT TTCTGCTGCC TTTTTCTTAC 
GCCCATACGG ACTTCTTCAC CTCAATTGGC CATATGACAG ACTTAATTAA TACAGAGAAA 
GATCTGGTGA TATCACTGAA AGATTACATC AAGGCAGAAG AAAGCAAGCT GGAACAGATC 
AAAAAATGGG CAGAGAAATT AGATAAGCTG ACAGATACTG CTACGAAAGA CCCTGAGGGG 
TTTTTGGGAC ATCCTGTAAA TGCATTCAAG CTGATGAAAC GGCTTAACAC GGAGTGGGGC 
GAGCTGGAGA GCCTGGTTCT GAAGGACATG TCAGATGGCT TCATCTCCAA CATGACGATC 
CAGCGGCAGT TTTTTCCAAA TGATGAAGAT CAGACTGGCG CAGCAAAAGC TCTCTTGCGG 
CTTCAAGACA CGTATAATTT GGATACAGAC ACCCTCTCAA GAGGGAATCT TCCAGGTGTA 
AAGCACAAAT CCTTTTTAAC AGCTGAAGAT TGCTTTGAGC TGGGAAAGAT TGCATACACG 
GAGGCCGATT ACTACCACAC TGAGCTCTGG ATGGAGCAAG CTCTGAAGCA GCTGGATGAG 
GGAGAAGTGT CTTCTGCAGA TAAAGTCTAT ATTCTGGACT ACCTGAGTTA TGCTGTGTAT 
CAGCAGGGGG ATCTGAGCAA AGCAATGATG CTCACCAAGC GCCTTCTCGA ACTGGATCCT 
GAGCATCAAA GAGCAAATGG AAACATGAAG TATTTTGAAT ATATCATGGC CAAAGAAAAA 
GAAGCCAATA AGTCCAGTAC AGATGCAGAA GACCAGACAG AGAAGGAAAC TGAGGTTAAG 
AAAAAGGATT ACCTGCCTGA GAGAAGGAAG TACGAAATGC TGTGCCGTGG GGAGGGTCTC 
AAAATGACTC CTCGGAGACA AAAAAGACTG TTCTGCCGCT ACTACGATGG AAACAGGAAT 
CCAAGATACA TTCTGGGCCC TGTCAAGCAG GAAGATGAGT GGGACAAGCC TCGAATTGTT 
CGCTTTCTTG ATATCATTTC CGATGAAGAG ATTGAAACTG TGAAAGAACT AGCAAAGCCA 
AGGCTGAGGC GAGCCACCAT TTCAAACCCC ATAACAGGAG CCTTGGAGAC GGCACATTAC 
AGAATTAGCA AAAGTGCTTG GTTGTCTGGA TATGAAAGCC CTGTGGTATC TCGCATTAAC 
ACCAGGATAC AGGACCTAAC AGGGCTCGAT GTCTCCACAG CAGAGGAACT TCAGGTGGCA 
AATTATGGAG TGGGAGGACA GTATGAACCC CACTTTGACT TTGCACGGAA AGACGAGCCA 
GATGCTTTTA AAGAATTGGG AACAGGCAAC AGAATTGCTA CTTGGTTGTT TTATATGAGC 
GACGTGTCAG CAGGGGGAGC TACCGTCTTT CCTGAAGTAG GAGCAAGCGT TTGGCCTAAA 
AAAGGAACAG CTGTATTCTG GTACAATCTC TTCCCAAGTG GAGAAGGAGA TTACAGCACA 
CGGCATGCAG CCTGCCCAGT ACTAGTTGGA AACAAATGGG TGTCCAATAA ATGGCTCCAC 
GAGCGGGGAC AGGAATTCCG AAGGCCGTGC ACGTTATCAG AGTTGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185667.1
CDS	106..1716
Translation	MASNKMWYTVALGFLLPFSYAHTDFFTSIGHMTDLINTEKDLVISLKDYIKAEESKLEQIKKWAEKLDKLTDTATKDPEGFLGHPVNAFKLMKRLNTEWGELESLVLKDMSDGFISNMTIQRQFFPNDEDQTGAAKALLRLQDTYNLDTDTLSRGNLPGVKHKSFLTAEDCFELGKIAYTEADYYHTELWMEQALKQLDEGEVSSADKVYILDYLSYAVYQQGDLSKAMMLTKRLLELDPEHQRANGNMKYFEYIMAKEKEANKSSTDAEDQTEKETEVKKKDYLPERRKYEMLCRGEGLKMTPRRQKRLFCRYYDGNRNPRYILGPVKQEDEWDKPRIVRFLDIISDEEIETVKELAKPRLRRATISNPITGALETAHYRISKSAWLSGYESPVVSRINTRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFPSGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE

Target ORF information:

RefSeq Version	NM_001198738.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus prolyl 4-hydroxylase subunit alpha 1 (P4HA1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198738.1

ORF Insert Sequence:

ATGGCATCCA ACAAGATGTG GTACACAGTT GCCCTTGGAT TTCTGCTGCC TTTTTCTTAC 
GCCCATACGG ACTTCTTCAC CTCAATTGGC CATATGACAG ACTTAATTAA TACAGAGAAA 
GATCTGGTGA TATCACTGAA AGATTACATC AAGGCAGAAG AAAGCAAGCT GGAACAGATC 
AAAAAATGGG CAGAGAAATT AGATAAGCTG ACAGATACTG CTACGAAAGA CCCTGAGGGG 
TTTTTGGGAC ATCCTGTAAA TGCATTCAAG CTGATGAAAC GGCTTAACAC GGAGTGGGGC 
GAGCTGGAGA GCCTGGTTCT GAAGGACATG TCAGATGGCT TCATCTCCAA CATGACGATC 
CAGCGGCAGT TTTTTCCAAA TGATGAAGAT CAGACTGGCG CAGCAAAAGC TCTCTTGCGG 
CTTCAAGACA CGTATAATTT GGATACAGAC ACCCTCTCAA GAGGGAATCT TCCAGGTGTA 
AAGCACAAAT CCTTTTTAAC AGCTGAAGAT TGCTTTGAGC TGGGAAAGAT TGCATACACG 
GAGGCCGATT ACTACCACAC TGAGCTCTGG ATGGAGCAAG CTCTGAAGCA GCTGGATGAG 
GGAGAAGTGT CTTCTGCAGA TAAAGTCTAT ATTCTGGACT ACCTGAGTTA TGCTGTGTAT 
CAGCAGGGGG ATCTGAGCAA AGCAATGATG CTCACCAAGC GCCTTCTCGA ACTGGATCCT 
GAGCATCAAA GAGCAAATGG AAACATGAAG TATTTTGAAT ATATCATGGC CAAAGAAAAA 
GAAGCCAATA AGTCCAGTAC AGATGCAGAA GACCAGACAG AGAAGGAAAC TGAGGTTAAG 
AAAAAGGATT ACCTGCCTGA GAGAAGGAAG TACGAAATGC TGTGCCGTGG GGAGGGTCTC 
AAAATGACTC CTCGGAGACA AAAAAGACTG TTCTGCCGCT ACTACGATGG AAACAGGAAT 
CCAAGATACA TTCTGGGCCC TGTCAAGCAG GAAGATGAGT GGGACAAGCC TCGAATTGTT 
CGCTTTCTTG ATATCATTTC CGATGAAGAG ATTGAAACTG TGAAAGAACT AGCAAAGCCA 
AGGCTGAGGC GAGCCACCAT TTCAAACCCC ATAACAGGAG CCTTGGAGAC GGCACATTAC 
AGAATTAGCA AAAGTGCTTG GTTGTCTGGA TATGAAAGCC CTGTGGTATC TCGCATTAAC 
ACCAGGATAC AGGACCTAAC AGGGCTCGAT GTCTCCACAG CAGAGGAACT TCAGGTGGCA 
AATTATGGAG TGGGAGGACA GTATGAACCC CACTTTGACT TTGCACGGAA AGACGAGCCA 
GATGCTTTTA AAGAATTGGG AACAGGCAAC AGAATTGCTA CTTGGTTGTT TTATATGAGC 
GACGTGTCAG CAGGGGGAGC TACCGTCTTT CCTGAAGTAG GAGCAAGCGT TTGGCCTAAA 
AAAGGAACAG CTGTATTCTG GTACAATCTC TTCCCAAGTG GAGAAGGAGA TTACAGCACA 
CGGCATGCAG CCTGCCCAGT ACTAGTTGGA AACAAATGGG TGTCCAATAA ATGGCTCCAC 
GAGCGGGGAC AGGAATTCCG AAGGCCGTGC ACGTTATCAG AGTTGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P4HA1 ( NM_001198736.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001185665.1 Gallus gallus prolyl 4-hydroxylase subunit alpha 1 (P4HA1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OGa00064
Clone ID Related Accession (Same CDS sequence)	NM_001198736.1
Accession Version	NM_001198736.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Gallus gallus(chicken)
Product	prolyl 4-hydroxylase subunit alpha-1 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000336.1. Transcript Variant: This variant (2) contains an alternate in-frame exon, compared to variant 1. It encodes isoform 2, which differs in sequence but is the same length as isoform 1. Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201357, SAMEA2201358 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCATCCA ACAAGATGTG GTACACAGTT GCCCTTGGAT TTCTGCTGCC TTTTTCTTAC 
GCCCATACGG ACTTCTTCAC CTCAATTGGC CATATGACAG ACTTAATTAA TACAGAGAAA 
GATCTGGTGA TATCACTGAA AGATTACATC AAGGCAGAAG AAAGCAAGCT GGAACAGATC 
AAAAAATGGG CAGAGAAATT AGATAAGCTG ACAGATACTG CTACGAAAGA CCCTGAGGGG 
TTTTTGGGAC ATCCTGTAAA TGCATTCAAG CTGATGAAAC GGCTTAACAC GGAGTGGGGC 
GAGCTGGAGA GCCTGGTTCT GAAGGACATG TCAGATGGCT TCATCTCCAA CATGACGATC 
CAGCGGCAGT TTTTTCCAAA TGATGAAGAT CAGACTGGCG CAGCAAAAGC TCTCTTGCGG 
CTTCAAGACA CGTATAATTT GGATACAGAC ACCCTCTCAA GAGGGAATCT TCCAGGTGTA 
AAGCACAAAT CCTTTTTAAC AGCTGAAGAT TGCTTTGAGC TGGGAAAGAT TGCATACACG 
GAGGCCGATT ACTACCACAC TGAGCTCTGG ATGGAGCAAG CTCTGAAGCA GCTGGATGAG 
GGAGAAGTGT CTTCTGCAGA TAAAGTCTAT ATTCTGGACT ACCTGAGTTA TGCTGTGTAT 
CAGCAGGGGG ATCTGAGCAA AGCAATGATG CTCACCAAGC GCCTTCTCGA ACTGGATCCT 
GAGCATCAAA GAGCAAATGG AAACATGAAG TATTTTGAAT ATATCATGGC CAAAGAAAAA 
GAAGCCAATA AGTCCAGTAC AGATGCAGAA GACCAGACAG AGAAGGAAAC TGAGGTTAAG 
AAAAAGGATT ACCTGCCTGA GAGAAGGAAG TACGAAATGC TGTGCCGTGG GGAGGGTCTC 
AAAATGACTC CTCGGAGACA AAAAAGACTG TTCTGCCGCT ACTACGATGG AAACAGGAAT 
CCAAGATACA TTCTGGGCCC TGTCAAGCAG GAAGATGAGT GGGACAAGCC TCGAATTGTT 
CGCTTTCTTG ATATCATTTC CGATGAAGAG ATTGAAACTG TGAAAGAACT AGCAAAGCCA 
AGGCTGAGCC GTGCTACTGT TCATGACCCC GAGACTGGGA AACTGACCAC AGCACATTAC 
AGAGTCTCTA AGAGTGCTTG GTTGTCTGGA TATGAAAGCC CTGTGGTATC TCGCATTAAC 
ACCAGGATAC AGGACCTAAC AGGGCTCGAT GTCTCCACAG CAGAGGAACT TCAGGTGGCA 
AATTATGGAG TGGGAGGACA GTATGAACCC CACTTTGACT TTGCACGGAA AGACGAGCCA 
GATGCTTTTA AAGAATTGGG AACAGGCAAC AGAATTGCTA CTTGGTTGTT TTATATGAGC 
GACGTGTCAG CAGGGGGAGC TACCGTCTTT CCTGAAGTAG GAGCAAGCGT TTGGCCTAAA 
AAAGGAACAG CTGTATTCTG GTACAATCTC TTCCCAAGTG GAGAAGGAGA TTACAGCACA 
CGGCATGCAG CCTGCCCAGT ACTAGTTGGA AACAAATGGG TGTCCAATAA ATGGCTCCAC 
GAGCGGGGAC AGGAATTCCG AAGGCCGTGC ACGTTATCAG AGTTGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185665.1
CDS	106..1716
Translation	MASNKMWYTVALGFLLPFSYAHTDFFTSIGHMTDLINTEKDLVISLKDYIKAEESKLEQIKKWAEKLDKLTDTATKDPEGFLGHPVNAFKLMKRLNTEWGELESLVLKDMSDGFISNMTIQRQFFPNDEDQTGAAKALLRLQDTYNLDTDTLSRGNLPGVKHKSFLTAEDCFELGKIAYTEADYYHTELWMEQALKQLDEGEVSSADKVYILDYLSYAVYQQGDLSKAMMLTKRLLELDPEHQRANGNMKYFEYIMAKEKEANKSSTDAEDQTEKETEVKKKDYLPERRKYEMLCRGEGLKMTPRRQKRLFCRYYDGNRNPRYILGPVKQEDEWDKPRIVRFLDIISDEEIETVKELAKPRLSRATVHDPETGKLTTAHYRVSKSAWLSGYESPVVSRINTRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFPSGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE

Target ORF information:

RefSeq Version	NM_001198736.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus prolyl 4-hydroxylase subunit alpha 1 (P4HA1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198736.1

ORF Insert Sequence:

ATGGCATCCA ACAAGATGTG GTACACAGTT GCCCTTGGAT TTCTGCTGCC TTTTTCTTAC 
GCCCATACGG ACTTCTTCAC CTCAATTGGC CATATGACAG ACTTAATTAA TACAGAGAAA 
GATCTGGTGA TATCACTGAA AGATTACATC AAGGCAGAAG AAAGCAAGCT GGAACAGATC 
AAAAAATGGG CAGAGAAATT AGATAAGCTG ACAGATACTG CTACGAAAGA CCCTGAGGGG 
TTTTTGGGAC ATCCTGTAAA TGCATTCAAG CTGATGAAAC GGCTTAACAC GGAGTGGGGC 
GAGCTGGAGA GCCTGGTTCT GAAGGACATG TCAGATGGCT TCATCTCCAA CATGACGATC 
CAGCGGCAGT TTTTTCCAAA TGATGAAGAT CAGACTGGCG CAGCAAAAGC TCTCTTGCGG 
CTTCAAGACA CGTATAATTT GGATACAGAC ACCCTCTCAA GAGGGAATCT TCCAGGTGTA 
AAGCACAAAT CCTTTTTAAC AGCTGAAGAT TGCTTTGAGC TGGGAAAGAT TGCATACACG 
GAGGCCGATT ACTACCACAC TGAGCTCTGG ATGGAGCAAG CTCTGAAGCA GCTGGATGAG 
GGAGAAGTGT CTTCTGCAGA TAAAGTCTAT ATTCTGGACT ACCTGAGTTA TGCTGTGTAT 
CAGCAGGGGG ATCTGAGCAA AGCAATGATG CTCACCAAGC GCCTTCTCGA ACTGGATCCT 
GAGCATCAAA GAGCAAATGG AAACATGAAG TATTTTGAAT ATATCATGGC CAAAGAAAAA 
GAAGCCAATA AGTCCAGTAC AGATGCAGAA GACCAGACAG AGAAGGAAAC TGAGGTTAAG 
AAAAAGGATT ACCTGCCTGA GAGAAGGAAG TACGAAATGC TGTGCCGTGG GGAGGGTCTC 
AAAATGACTC CTCGGAGACA AAAAAGACTG TTCTGCCGCT ACTACGATGG AAACAGGAAT 
CCAAGATACA TTCTGGGCCC TGTCAAGCAG GAAGATGAGT GGGACAAGCC TCGAATTGTT 
CGCTTTCTTG ATATCATTTC CGATGAAGAG ATTGAAACTG TGAAAGAACT AGCAAAGCCA 
AGGCTGAGCC GTGCTACTGT TCATGACCCC GAGACTGGGA AACTGACCAC AGCACATTAC 
AGAGTCTCTA AGAGTGCTTG GTTGTCTGGA TATGAAAGCC CTGTGGTATC TCGCATTAAC 
ACCAGGATAC AGGACCTAAC AGGGCTCGAT GTCTCCACAG CAGAGGAACT TCAGGTGGCA 
AATTATGGAG TGGGAGGACA GTATGAACCC CACTTTGACT TTGCACGGAA AGACGAGCCA 
GATGCTTTTA AAGAATTGGG AACAGGCAAC AGAATTGCTA CTTGGTTGTT TTATATGAGC 
GACGTGTCAG CAGGGGGAGC TACCGTCTTT CCTGAAGTAG GAGCAAGCGT TTGGCCTAAA 
AAAGGAACAG CTGTATTCTG GTACAATCTC TTCCCAAGTG GAGAAGGAGA TTACAGCACA 
CGGCATGCAG CCTGCCCAGT ACTAGTTGGA AACAAATGGG TGTCCAATAA ATGGCTCCAC 
GAGCGGGGAC AGGAATTCCG AAGGCCGTGC ACGTTATCAG AGTTGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P4HA1 ( NM_001198737.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001185666.1 Gallus gallus prolyl 4-hydroxylase subunit alpha 1 (P4HA1), transcript variant 3, mRNA.

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CloneID	OGa00084
Clone ID Related Accession (Same CDS sequence)	XM_015288205.1 , NM_001198737.1
Accession Version	NM_001198737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Gallus gallus(chicken)
Product	prolyl 4-hydroxylase subunit alpha-1 isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000336.1. Transcript Variant: This variant (3) contains an alternate in-frame exon, compared to variant 1. It encodes isoform 3, which differs in sequence but is the same length as isoform 1. Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: HAEL01005850.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2201357, SAMEA2201358 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCATCCA ACAAGATGTG GTACACAGTT GCCCTTGGAT TTCTGCTGCC TTTTTCTTAC 
GCCCATACGG ACTTCTTCAC CTCAATTGGC CATATGACAG ACTTAATTAA TACAGAGAAA 
GATCTGGTGA TATCACTGAA AGATTACATC AAGGCAGAAG AAAGCAAGCT GGAACAGATC 
AAAAAATGGG CAGAGAAATT AGATAAGCTG ACAGATACTG CTACGAAAGA CCCTGAGGGG 
TTTTTGGGAC ATCCTGTAAA TGCATTCAAG CTGATGAAAC GGCTTAACAC GGAGTGGGGC 
GAGCTGGAGA GCCTGGTTCT GAAGGACATG TCAGATGGCT TCATCTCCAA CATGACGATC 
CAGCGGCAGT TTTTTCCAAA TGATGAAGAT CAGACTGGCG CAGCAAAAGC TCTCTTGCGG 
CTTCAAGACA CGTATAATTT GGATACAGAC ACCCTCTCAA GAGGGAATCT TCCAGGTGTA 
AAGCACAAAT CCTTTTTAAC AGCTGAAGAT TGCTTTGAGC TGGGAAAGAT TGCATACACG 
GAGGCCGATT ACTACCACAC TGAGCTCTGG ATGGAGCAAG CTCTGAAGCA GCTGGATGAG 
GGAGAAGTGT CTTCTGCAGA TAAAGTCTAT ATTCTGGACT ACCTGAGTTA TGCTGTGTAT 
CAGCAGGGGG ATCTGAGCAA AGCAATGATG CTCACCAAGC GCCTTCTCGA ACTGGATCCT 
GAGCATCAAA GAGCAAATGG AAACATGAAG TATTTTGAAT ATATCATGGC CAAAGAAAAA 
GAAGCCAATA AGTCCAGTAC AGATGCAGAA GACCAGACAG AGAAGGAAAC TGAGGTTAAG 
AAAAAGGATT ACCTGCCTGA GAGAAGGAAG TACGAAATGC TGTGCCGTGG GGAGGGTCTC 
AAAATGACTC CTCGGAGACA AAAAAGACTG TTCTGCCGCT ACTACGATGG AAACAGGAAT 
CCAAGATACA TTCTGGGCCC TGTCAAGCAG GAAGATGAGT GGGACAAGCC TCGAATTGTT 
CGCTTTCTTG ATATCATTTC CGATGAAGAG ATTGAAACTG TGAAAGAACT AGCAAAGCCA 
AGGCTGAGGC GAGCCACCAT TTCAAACCCC ATAACAGGAG CCTTGGAGAC GGCACATTAC 
AGAATTAGCA AAAGTGCTTG GTTGTCTGGA TATGAAAGCC CTGTGGTATC TCGCATTAAC 
ACCAGGATAC AGGACCTAAC AGGGCTCGAT GTCTCCACAG CAGAGGAACT TCAGGTAGCC 
AACTACGGCG TAGGAGGCCA GTATGAGCCT CATTTTGATT TCGGAAGGAA AGACGAGCCA 
GATGCTTTTA AAGAATTGGG AACAGGCAAC AGAATTGCTA CTTGGTTGTT TTATATGAGC 
GACGTGTCAG CAGGGGGAGC TACCGTCTTT CCTGAAGTAG GAGCAAGCGT TTGGCCTAAA 
AAAGGAACAG CTGTATTCTG GTACAATCTC TTCCCAAGTG GAGAAGGAGA TTACAGCACA 
CGGCATGCAG CCTGCCCAGT ACTAGTTGGA AACAAATGGG TGTCCAATAA ATGGCTCCAC 
GAGCGGGGAC AGGAATTCCG AAGGCCGTGC ACGTTATCAG AGTTGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185666.1
CDS	106..1716
Translation	MASNKMWYTVALGFLLPFSYAHTDFFTSIGHMTDLINTEKDLVISLKDYIKAEESKLEQIKKWAEKLDKLTDTATKDPEGFLGHPVNAFKLMKRLNTEWGELESLVLKDMSDGFISNMTIQRQFFPNDEDQTGAAKALLRLQDTYNLDTDTLSRGNLPGVKHKSFLTAEDCFELGKIAYTEADYYHTELWMEQALKQLDEGEVSSADKVYILDYLSYAVYQQGDLSKAMMLTKRLLELDPEHQRANGNMKYFEYIMAKEKEANKSSTDAEDQTEKETEVKKKDYLPERRKYEMLCRGEGLKMTPRRQKRLFCRYYDGNRNPRYILGPVKQEDEWDKPRIVRFLDIISDEEIETVKELAKPRLRRATISNPITGALETAHYRISKSAWLSGYESPVVSRINTRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFGRKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFPSGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE

Target ORF information:

RefSeq Version	NM_001198737.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus prolyl 4-hydroxylase subunit alpha 1 (P4HA1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198737.1

ORF Insert Sequence:

ATGGCATCCA ACAAGATGTG GTACACAGTT GCCCTTGGAT TTCTGCTGCC TTTTTCTTAC 
GCCCATACGG ACTTCTTCAC CTCAATTGGC CATATGACAG ACTTAATTAA TACAGAGAAA 
GATCTGGTGA TATCACTGAA AGATTACATC AAGGCAGAAG AAAGCAAGCT GGAACAGATC 
AAAAAATGGG CAGAGAAATT AGATAAGCTG ACAGATACTG CTACGAAAGA CCCTGAGGGG 
TTTTTGGGAC ATCCTGTAAA TGCATTCAAG CTGATGAAAC GGCTTAACAC GGAGTGGGGC 
GAGCTGGAGA GCCTGGTTCT GAAGGACATG TCAGATGGCT TCATCTCCAA CATGACGATC 
CAGCGGCAGT TTTTTCCAAA TGATGAAGAT CAGACTGGCG CAGCAAAAGC TCTCTTGCGG 
CTTCAAGACA CGTATAATTT GGATACAGAC ACCCTCTCAA GAGGGAATCT TCCAGGTGTA 
AAGCACAAAT CCTTTTTAAC AGCTGAAGAT TGCTTTGAGC TGGGAAAGAT TGCATACACG 
GAGGCCGATT ACTACCACAC TGAGCTCTGG ATGGAGCAAG CTCTGAAGCA GCTGGATGAG 
GGAGAAGTGT CTTCTGCAGA TAAAGTCTAT ATTCTGGACT ACCTGAGTTA TGCTGTGTAT 
CAGCAGGGGG ATCTGAGCAA AGCAATGATG CTCACCAAGC GCCTTCTCGA ACTGGATCCT 
GAGCATCAAA GAGCAAATGG AAACATGAAG TATTTTGAAT ATATCATGGC CAAAGAAAAA 
GAAGCCAATA AGTCCAGTAC AGATGCAGAA GACCAGACAG AGAAGGAAAC TGAGGTTAAG 
AAAAAGGATT ACCTGCCTGA GAGAAGGAAG TACGAAATGC TGTGCCGTGG GGAGGGTCTC 
AAAATGACTC CTCGGAGACA AAAAAGACTG TTCTGCCGCT ACTACGATGG AAACAGGAAT 
CCAAGATACA TTCTGGGCCC TGTCAAGCAG GAAGATGAGT GGGACAAGCC TCGAATTGTT 
CGCTTTCTTG ATATCATTTC CGATGAAGAG ATTGAAACTG TGAAAGAACT AGCAAAGCCA 
AGGCTGAGGC GAGCCACCAT TTCAAACCCC ATAACAGGAG CCTTGGAGAC GGCACATTAC 
AGAATTAGCA AAAGTGCTTG GTTGTCTGGA TATGAAAGCC CTGTGGTATC TCGCATTAAC 
ACCAGGATAC AGGACCTAAC AGGGCTCGAT GTCTCCACAG CAGAGGAACT TCAGGTAGCC 
AACTACGGCG TAGGAGGCCA GTATGAGCCT CATTTTGATT TCGGAAGGAA AGACGAGCCA 
GATGCTTTTA AAGAATTGGG AACAGGCAAC AGAATTGCTA CTTGGTTGTT TTATATGAGC 
GACGTGTCAG CAGGGGGAGC TACCGTCTTT CCTGAAGTAG GAGCAAGCGT TTGGCCTAAA 
AAAGGAACAG CTGTATTCTG GTACAATCTC TTCCCAAGTG GAGAAGGAGA TTACAGCACA 
CGGCATGCAG CCTGCCCAGT ACTAGTTGGA AACAAATGGG TGTCCAATAA ATGGCTCCAC 
GAGCGGGGAC AGGAATTCCG AAGGCCGTGC ACGTTATCAG AGTTGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P4HA1 ( XM_025151565.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025007333.1 Gallus gallus prolyl 4-hydroxylase subunit alpha 1 (P4HA1), transcript variant X2, mRNA.

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CloneID	OGa00008
Clone ID Related Accession (Same CDS sequence)	XM_025151565.1 , XM_025151564.1 , NM_001198735.1
Accession Version	XM_025151565.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	prolyl 4-hydroxylase subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCCA ACAAGATGTG GTACACAGTT GCCCTTGGAT TTCTGCTGCC TTTTTCTTAC 
GCCCATACGG ACTTCTTCAC CTCAATTGGC CATATGACAG ACTTAATTAA TACAGAGAAA 
GATCTGGTGA TATCACTGAA AGATTACATC AAGGCAGAAG AAAGCAAGCT GGAACAGATC 
AAAAAATGGG CAGAGAAATT AGATAAGCTG ACAGATACTG CTACGAAAGA CCCTGAGGGG 
TTTTTGGGAC ATCCTGTAAA TGCATTCAAG CTGATGAAAC GGCTTAACAC GGAGTGGGGC 
GAGCTGGAGA GCCTGGTTCT GAAGGACATG TCAGATGGCT TCATCTCCAA CATGACGATC 
CAGCGGCAGT TTTTTCCAAA TGATGAAGAT CAGACTGGCG CAGCAAAAGC TCTCTTGCGG 
CTTCAAGACA CGTATAATTT GGATACAGAC ACCCTCTCAA GAGGGAATCT TCCAGGTGTA 
AAGCACAAAT CCTTTTTAAC AGCTGAAGAT TGCTTTGAGC TGGGAAAGAT TGCATACACG 
GAGGCCGATT ACTACCACAC TGAGCTCTGG ATGGAGCAAG CTCTGAAGCA GCTGGATGAG 
GGAGAAGTGT CTTCTGCAGA TAAAGTCTAT ATTCTGGACT ACCTGAGTTA TGCTGTGTAT 
CAGCAGGGGG ATCTGAGCAA AGCAATGATG CTCACCAAGC GCCTTCTCGA ACTGGATCCT 
GAGCATCAAA GAGCAAATGG AAACATGAAG TATTTTGAAT ATATCATGGC CAAAGAAAAA 
GAAGCCAATA AGTCCAGTAC AGATGCAGAA GACCAGACAG AGAAGGAAAC TGAGGTTAAG 
AAAAAGGATT ACCTGCCTGA GAGAAGGAAG TACGAAATGC TGTGCCGTGG GGAGGGTCTC 
AAAATGACTC CTCGGAGACA AAAAAGACTG TTCTGCCGCT ACTACGATGG AAACAGGAAT 
CCAAGATACA TTCTGGGCCC TGTCAAGCAG GAAGATGAGT GGGACAAGCC TCGAATTGTT 
CGCTTTCTTG ATATCATTTC CGATGAAGAG ATTGAAACTG TGAAAGAACT AGCAAAGCCA 
AGGCTGAGCC GTGCTACTGT TCATGACCCC GAGACTGGGA AACTGACCAC AGCACATTAC 
AGAGTCTCTA AGAGTGCTTG GTTGTCTGGA TATGAAAGCC CTGTGGTATC TCGCATTAAC 
ACCAGGATAC AGGACCTAAC AGGGCTCGAT GTCTCCACAG CAGAGGAACT TCAGGTAGCC 
AACTACGGCG TAGGAGGCCA GTATGAGCCT CATTTTGATT TCGGAAGGAA AGACGAGCCA 
GATGCTTTTA AAGAATTGGG AACAGGCAAC AGAATTGCTA CTTGGTTGTT TTATATGAGC 
GACGTGTCAG CAGGGGGAGC TACCGTCTTT CCTGAAGTAG GAGCAAGCGT TTGGCCTAAA 
AAAGGAACAG CTGTATTCTG GTACAATCTC TTCCCAAGTG GAGAAGGAGA TTACAGCACA 
CGGCATGCAG CCTGCCCAGT ACTAGTTGGA AACAAATGGG TGTCCAATAA ATGGCTCCAC 
GAGCGGGGAC AGGAATTCCG AAGGCCGTGC ACGTTATCAG AGTTGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025007333.1
CDS	137..1747
Translation	MASNKMWYTVALGFLLPFSYAHTDFFTSIGHMTDLINTEKDLVISLKDYIKAEESKLEQIKKWAEKLDKLTDTATKDPEGFLGHPVNAFKLMKRLNTEWGELESLVLKDMSDGFISNMTIQRQFFPNDEDQTGAAKALLRLQDTYNLDTDTLSRGNLPGVKHKSFLTAEDCFELGKIAYTEADYYHTELWMEQALKQLDEGEVSSADKVYILDYLSYAVYQQGDLSKAMMLTKRLLELDPEHQRANGNMKYFEYIMAKEKEANKSSTDAEDQTEKETEVKKKDYLPERRKYEMLCRGEGLKMTPRRQKRLFCRYYDGNRNPRYILGPVKQEDEWDKPRIVRFLDIISDEEIETVKELAKPRLSRATVHDPETGKLTTAHYRVSKSAWLSGYESPVVSRINTRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFGRKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFPSGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE

Target ORF information:

RefSeq Version	XM_025151565.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus prolyl 4-hydroxylase subunit alpha 1 (P4HA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025151565.1

ORF Insert Sequence:

ATGGCATCCA ACAAGATGTG GTACACAGTT GCCCTTGGAT TTCTGCTGCC TTTTTCTTAC 
GCCCATACGG ACTTCTTCAC CTCAATTGGC CATATGACAG ACTTAATTAA TACAGAGAAA 
GATCTGGTGA TATCACTGAA AGATTACATC AAGGCAGAAG AAAGCAAGCT GGAACAGATC 
AAAAAATGGG CAGAGAAATT AGATAAGCTG ACAGATACTG CTACGAAAGA CCCTGAGGGG 
TTTTTGGGAC ATCCTGTAAA TGCATTCAAG CTGATGAAAC GGCTTAACAC GGAGTGGGGC 
GAGCTGGAGA GCCTGGTTCT GAAGGACATG TCAGATGGCT TCATCTCCAA CATGACGATC 
CAGCGGCAGT TTTTTCCAAA TGATGAAGAT CAGACTGGCG CAGCAAAAGC TCTCTTGCGG 
CTTCAAGACA CGTATAATTT GGATACAGAC ACCCTCTCAA GAGGGAATCT TCCAGGTGTA 
AAGCACAAAT CCTTTTTAAC AGCTGAAGAT TGCTTTGAGC TGGGAAAGAT TGCATACACG 
GAGGCCGATT ACTACCACAC TGAGCTCTGG ATGGAGCAAG CTCTGAAGCA GCTGGATGAG 
GGAGAAGTGT CTTCTGCAGA TAAAGTCTAT ATTCTGGACT ACCTGAGTTA TGCTGTGTAT 
CAGCAGGGGG ATCTGAGCAA AGCAATGATG CTCACCAAGC GCCTTCTCGA ACTGGATCCT 
GAGCATCAAA GAGCAAATGG AAACATGAAG TATTTTGAAT ATATCATGGC CAAAGAAAAA 
GAAGCCAATA AGTCCAGTAC AGATGCAGAA GACCAGACAG AGAAGGAAAC TGAGGTTAAG 
AAAAAGGATT ACCTGCCTGA GAGAAGGAAG TACGAAATGC TGTGCCGTGG GGAGGGTCTC 
AAAATGACTC CTCGGAGACA AAAAAGACTG TTCTGCCGCT ACTACGATGG AAACAGGAAT 
CCAAGATACA TTCTGGGCCC TGTCAAGCAG GAAGATGAGT GGGACAAGCC TCGAATTGTT 
CGCTTTCTTG ATATCATTTC CGATGAAGAG ATTGAAACTG TGAAAGAACT AGCAAAGCCA 
AGGCTGAGCC GTGCTACTGT TCATGACCCC GAGACTGGGA AACTGACCAC AGCACATTAC 
AGAGTCTCTA AGAGTGCTTG GTTGTCTGGA TATGAAAGCC CTGTGGTATC TCGCATTAAC 
ACCAGGATAC AGGACCTAAC AGGGCTCGAT GTCTCCACAG CAGAGGAACT TCAGGTAGCC 
AACTACGGCG TAGGAGGCCA GTATGAGCCT CATTTTGATT TCGGAAGGAA AGACGAGCCA 
GATGCTTTTA AAGAATTGGG AACAGGCAAC AGAATTGCTA CTTGGTTGTT TTATATGAGC 
GACGTGTCAG CAGGGGGAGC TACCGTCTTT CCTGAAGTAG GAGCAAGCGT TTGGCCTAAA 
AAAGGAACAG CTGTATTCTG GTACAATCTC TTCCCAAGTG GAGAAGGAGA TTACAGCACA 
CGGCATGCAG CCTGCCCAGT ACTAGTTGGA AACAAATGGG TGTCCAATAA ATGGCTCCAC 
GAGCGGGGAC AGGAATTCCG AAGGCCGTGC ACGTTATCAG AGTTGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P4HA1 ( XM_025151564.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025007332.1 Gallus gallus prolyl 4-hydroxylase subunit alpha 1 (P4HA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa00008
Clone ID Related Accession (Same CDS sequence)	XM_025151565.1 , XM_025151564.1 , NM_001198735.1
Accession Version	XM_025151564.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	prolyl 4-hydroxylase subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCCA ACAAGATGTG GTACACAGTT GCCCTTGGAT TTCTGCTGCC TTTTTCTTAC 
GCCCATACGG ACTTCTTCAC CTCAATTGGC CATATGACAG ACTTAATTAA TACAGAGAAA 
GATCTGGTGA TATCACTGAA AGATTACATC AAGGCAGAAG AAAGCAAGCT GGAACAGATC 
AAAAAATGGG CAGAGAAATT AGATAAGCTG ACAGATACTG CTACGAAAGA CCCTGAGGGG 
TTTTTGGGAC ATCCTGTAAA TGCATTCAAG CTGATGAAAC GGCTTAACAC GGAGTGGGGC 
GAGCTGGAGA GCCTGGTTCT GAAGGACATG TCAGATGGCT TCATCTCCAA CATGACGATC 
CAGCGGCAGT TTTTTCCAAA TGATGAAGAT CAGACTGGCG CAGCAAAAGC TCTCTTGCGG 
CTTCAAGACA CGTATAATTT GGATACAGAC ACCCTCTCAA GAGGGAATCT TCCAGGTGTA 
AAGCACAAAT CCTTTTTAAC AGCTGAAGAT TGCTTTGAGC TGGGAAAGAT TGCATACACG 
GAGGCCGATT ACTACCACAC TGAGCTCTGG ATGGAGCAAG CTCTGAAGCA GCTGGATGAG 
GGAGAAGTGT CTTCTGCAGA TAAAGTCTAT ATTCTGGACT ACCTGAGTTA TGCTGTGTAT 
CAGCAGGGGG ATCTGAGCAA AGCAATGATG CTCACCAAGC GCCTTCTCGA ACTGGATCCT 
GAGCATCAAA GAGCAAATGG AAACATGAAG TATTTTGAAT ATATCATGGC CAAAGAAAAA 
GAAGCCAATA AGTCCAGTAC AGATGCAGAA GACCAGACAG AGAAGGAAAC TGAGGTTAAG 
AAAAAGGATT ACCTGCCTGA GAGAAGGAAG TACGAAATGC TGTGCCGTGG GGAGGGTCTC 
AAAATGACTC CTCGGAGACA AAAAAGACTG TTCTGCCGCT ACTACGATGG AAACAGGAAT 
CCAAGATACA TTCTGGGCCC TGTCAAGCAG GAAGATGAGT GGGACAAGCC TCGAATTGTT 
CGCTTTCTTG ATATCATTTC CGATGAAGAG ATTGAAACTG TGAAAGAACT AGCAAAGCCA 
AGGCTGAGCC GTGCTACTGT TCATGACCCC GAGACTGGGA AACTGACCAC AGCACATTAC 
AGAGTCTCTA AGAGTGCTTG GTTGTCTGGA TATGAAAGCC CTGTGGTATC TCGCATTAAC 
ACCAGGATAC AGGACCTAAC AGGGCTCGAT GTCTCCACAG CAGAGGAACT TCAGGTAGCC 
AACTACGGCG TAGGAGGCCA GTATGAGCCT CATTTTGATT TCGGAAGGAA AGACGAGCCA 
GATGCTTTTA AAGAATTGGG AACAGGCAAC AGAATTGCTA CTTGGTTGTT TTATATGAGC 
GACGTGTCAG CAGGGGGAGC TACCGTCTTT CCTGAAGTAG GAGCAAGCGT TTGGCCTAAA 
AAAGGAACAG CTGTATTCTG GTACAATCTC TTCCCAAGTG GAGAAGGAGA TTACAGCACA 
CGGCATGCAG CCTGCCCAGT ACTAGTTGGA AACAAATGGG TGTCCAATAA ATGGCTCCAC 
GAGCGGGGAC AGGAATTCCG AAGGCCGTGC ACGTTATCAG AGTTGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025007332.1
CDS	170..1780
Translation	MASNKMWYTVALGFLLPFSYAHTDFFTSIGHMTDLINTEKDLVISLKDYIKAEESKLEQIKKWAEKLDKLTDTATKDPEGFLGHPVNAFKLMKRLNTEWGELESLVLKDMSDGFISNMTIQRQFFPNDEDQTGAAKALLRLQDTYNLDTDTLSRGNLPGVKHKSFLTAEDCFELGKIAYTEADYYHTELWMEQALKQLDEGEVSSADKVYILDYLSYAVYQQGDLSKAMMLTKRLLELDPEHQRANGNMKYFEYIMAKEKEANKSSTDAEDQTEKETEVKKKDYLPERRKYEMLCRGEGLKMTPRRQKRLFCRYYDGNRNPRYILGPVKQEDEWDKPRIVRFLDIISDEEIETVKELAKPRLSRATVHDPETGKLTTAHYRVSKSAWLSGYESPVVSRINTRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFGRKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFPSGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE

Target ORF information:

RefSeq Version	XM_025151564.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus prolyl 4-hydroxylase subunit alpha 1 (P4HA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025151564.1

ORF Insert Sequence:

ATGGCATCCA ACAAGATGTG GTACACAGTT GCCCTTGGAT TTCTGCTGCC TTTTTCTTAC 
GCCCATACGG ACTTCTTCAC CTCAATTGGC CATATGACAG ACTTAATTAA TACAGAGAAA 
GATCTGGTGA TATCACTGAA AGATTACATC AAGGCAGAAG AAAGCAAGCT GGAACAGATC 
AAAAAATGGG CAGAGAAATT AGATAAGCTG ACAGATACTG CTACGAAAGA CCCTGAGGGG 
TTTTTGGGAC ATCCTGTAAA TGCATTCAAG CTGATGAAAC GGCTTAACAC GGAGTGGGGC 
GAGCTGGAGA GCCTGGTTCT GAAGGACATG TCAGATGGCT TCATCTCCAA CATGACGATC 
CAGCGGCAGT TTTTTCCAAA TGATGAAGAT CAGACTGGCG CAGCAAAAGC TCTCTTGCGG 
CTTCAAGACA CGTATAATTT GGATACAGAC ACCCTCTCAA GAGGGAATCT TCCAGGTGTA 
AAGCACAAAT CCTTTTTAAC AGCTGAAGAT TGCTTTGAGC TGGGAAAGAT TGCATACACG 
GAGGCCGATT ACTACCACAC TGAGCTCTGG ATGGAGCAAG CTCTGAAGCA GCTGGATGAG 
GGAGAAGTGT CTTCTGCAGA TAAAGTCTAT ATTCTGGACT ACCTGAGTTA TGCTGTGTAT 
CAGCAGGGGG ATCTGAGCAA AGCAATGATG CTCACCAAGC GCCTTCTCGA ACTGGATCCT 
GAGCATCAAA GAGCAAATGG AAACATGAAG TATTTTGAAT ATATCATGGC CAAAGAAAAA 
GAAGCCAATA AGTCCAGTAC AGATGCAGAA GACCAGACAG AGAAGGAAAC TGAGGTTAAG 
AAAAAGGATT ACCTGCCTGA GAGAAGGAAG TACGAAATGC TGTGCCGTGG GGAGGGTCTC 
AAAATGACTC CTCGGAGACA AAAAAGACTG TTCTGCCGCT ACTACGATGG AAACAGGAAT 
CCAAGATACA TTCTGGGCCC TGTCAAGCAG GAAGATGAGT GGGACAAGCC TCGAATTGTT 
CGCTTTCTTG ATATCATTTC CGATGAAGAG ATTGAAACTG TGAAAGAACT AGCAAAGCCA 
AGGCTGAGCC GTGCTACTGT TCATGACCCC GAGACTGGGA AACTGACCAC AGCACATTAC 
AGAGTCTCTA AGAGTGCTTG GTTGTCTGGA TATGAAAGCC CTGTGGTATC TCGCATTAAC 
ACCAGGATAC AGGACCTAAC AGGGCTCGAT GTCTCCACAG CAGAGGAACT TCAGGTAGCC 
AACTACGGCG TAGGAGGCCA GTATGAGCCT CATTTTGATT TCGGAAGGAA AGACGAGCCA 
GATGCTTTTA AAGAATTGGG AACAGGCAAC AGAATTGCTA CTTGGTTGTT TTATATGAGC 
GACGTGTCAG CAGGGGGAGC TACCGTCTTT CCTGAAGTAG GAGCAAGCGT TTGGCCTAAA 
AAAGGAACAG CTGTATTCTG GTACAATCTC TTCCCAAGTG GAGAAGGAGA TTACAGCACA 
CGGCATGCAG CCTGCCCAGT ACTAGTTGGA AACAAATGGG TGTCCAATAA ATGGCTCCAC 
GAGCGGGGAC AGGAATTCCG AAGGCCGTGC ACGTTATCAG AGTTGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P4HA1 ( XM_015288205.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015143691.1 Gallus gallus prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant X1, mRNA.

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CloneID	OGa00084
Clone ID Related Accession (Same CDS sequence)	XM_015288205.1 , NM_001198737.1
Accession Version	XM_015288205.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	prolyl 4-hydroxylase subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCCA ACAAGATGTG GTACACAGTT GCCCTTGGAT TTCTGCTGCC TTTTTCTTAC 
GCCCATACGG ACTTCTTCAC CTCAATTGGC CATATGACAG ACTTAATTAA TACAGAGAAA 
GATCTGGTGA TATCACTGAA AGATTACATC AAGGCAGAAG AAAGCAAGCT GGAACAGATC 
AAAAAATGGG CAGAGAAATT AGATAAGCTG ACAGATACTG CTACGAAAGA CCCTGAGGGG 
TTTTTGGGAC ATCCTGTAAA TGCATTCAAG CTGATGAAAC GGCTTAACAC GGAGTGGGGC 
GAGCTGGAGA GCCTGGTTCT GAAGGACATG TCAGATGGCT TCATCTCCAA CATGACGATC 
CAGCGGCAGT TTTTTCCAAA TGATGAAGAT CAGACTGGCG CAGCAAAAGC TCTCTTGCGG 
CTTCAAGACA CGTATAATTT GGATACAGAC ACCCTCTCAA GAGGGAATCT TCCAGGTGTA 
AAGCACAAAT CCTTTTTAAC AGCTGAAGAT TGCTTTGAGC TGGGAAAGAT TGCATACACG 
GAGGCCGATT ACTACCACAC TGAGCTCTGG ATGGAGCAAG CTCTGAAGCA GCTGGATGAG 
GGAGAAGTGT CTTCTGCAGA TAAAGTCTAT ATTCTGGACT ACCTGAGTTA TGCTGTGTAT 
CAGCAGGGGG ATCTGAGCAA AGCAATGATG CTCACCAAGC GCCTTCTCGA ACTGGATCCT 
GAGCATCAAA GAGCAAATGG AAACATGAAG TATTTTGAAT ATATCATGGC CAAAGAAAAA 
GAAGCCAATA AGTCCAGTAC AGATGCAGAA GACCAGACAG AGAAGGAAAC TGAGGTTAAG 
AAAAAGGATT ACCTGCCTGA GAGAAGGAAG TACGAAATGC TGTGCCGTGG GGAGGGTCTC 
AAAATGACTC CTCGGAGACA AAAAAGACTG TTCTGCCGCT ACTACGATGG AAACAGGAAT 
CCAAGATACA TTCTGGGCCC TGTCAAGCAG GAAGATGAGT GGGACAAGCC TCGAATTGTT 
CGCTTTCTTG ATATCATTTC CGATGAAGAG ATTGAAACTG TGAAAGAACT AGCAAAGCCA 
AGGCTGAGGC GAGCCACCAT TTCAAACCCC ATAACAGGAG CCTTGGAGAC GGCACATTAC 
AGAATTAGCA AAAGTGCTTG GTTGTCTGGA TATGAAAGCC CTGTGGTATC TCGCATTAAC 
ACCAGGATAC AGGACCTAAC AGGGCTCGAT GTCTCCACAG CAGAGGAACT TCAGGTAGCC 
AACTACGGCG TAGGAGGCCA GTATGAGCCT CATTTTGATT TCGGAAGGAA AGACGAGCCA 
GATGCTTTTA AAGAATTGGG AACAGGCAAC AGAATTGCTA CTTGGTTGTT TTATATGAGC 
GACGTGTCAG CAGGGGGAGC TACCGTCTTT CCTGAAGTAG GAGCAAGCGT TTGGCCTAAA 
AAAGGAACAG CTGTATTCTG GTACAATCTC TTCCCAAGTG GAGAAGGAGA TTACAGCACA 
CGGCATGCAG CCTGCCCAGT ACTAGTTGGA AACAAATGGG TGTCCAATAA ATGGCTCCAC 
GAGCGGGGAC AGGAATTCCG AAGGCCGTGC ACGTTATCAG AGTTGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015143691.1
CDS	128..1738
Translation	MASNKMWYTVALGFLLPFSYAHTDFFTSIGHMTDLINTEKDLVISLKDYIKAEESKLEQIKKWAEKLDKLTDTATKDPEGFLGHPVNAFKLMKRLNTEWGELESLVLKDMSDGFISNMTIQRQFFPNDEDQTGAAKALLRLQDTYNLDTDTLSRGNLPGVKHKSFLTAEDCFELGKIAYTEADYYHTELWMEQALKQLDEGEVSSADKVYILDYLSYAVYQQGDLSKAMMLTKRLLELDPEHQRANGNMKYFEYIMAKEKEANKSSTDAEDQTEKETEVKKKDYLPERRKYEMLCRGEGLKMTPRRQKRLFCRYYDGNRNPRYILGPVKQEDEWDKPRIVRFLDIISDEEIETVKELAKPRLRRATISNPITGALETAHYRISKSAWLSGYESPVVSRINTRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFGRKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFPSGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE

Target ORF information:

RefSeq Version	XM_015288205.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015288205.1

ORF Insert Sequence:

ATGGCATCCA ACAAGATGTG GTACACAGTT GCCCTTGGAT TTCTGCTGCC TTTTTCTTAC 
GCCCATACGG ACTTCTTCAC CTCAATTGGC CATATGACAG ACTTAATTAA TACAGAGAAA 
GATCTGGTGA TATCACTGAA AGATTACATC AAGGCAGAAG AAAGCAAGCT GGAACAGATC 
AAAAAATGGG CAGAGAAATT AGATAAGCTG ACAGATACTG CTACGAAAGA CCCTGAGGGG 
TTTTTGGGAC ATCCTGTAAA TGCATTCAAG CTGATGAAAC GGCTTAACAC GGAGTGGGGC 
GAGCTGGAGA GCCTGGTTCT GAAGGACATG TCAGATGGCT TCATCTCCAA CATGACGATC 
CAGCGGCAGT TTTTTCCAAA TGATGAAGAT CAGACTGGCG CAGCAAAAGC TCTCTTGCGG 
CTTCAAGACA CGTATAATTT GGATACAGAC ACCCTCTCAA GAGGGAATCT TCCAGGTGTA 
AAGCACAAAT CCTTTTTAAC AGCTGAAGAT TGCTTTGAGC TGGGAAAGAT TGCATACACG 
GAGGCCGATT ACTACCACAC TGAGCTCTGG ATGGAGCAAG CTCTGAAGCA GCTGGATGAG 
GGAGAAGTGT CTTCTGCAGA TAAAGTCTAT ATTCTGGACT ACCTGAGTTA TGCTGTGTAT 
CAGCAGGGGG ATCTGAGCAA AGCAATGATG CTCACCAAGC GCCTTCTCGA ACTGGATCCT 
GAGCATCAAA GAGCAAATGG AAACATGAAG TATTTTGAAT ATATCATGGC CAAAGAAAAA 
GAAGCCAATA AGTCCAGTAC AGATGCAGAA GACCAGACAG AGAAGGAAAC TGAGGTTAAG 
AAAAAGGATT ACCTGCCTGA GAGAAGGAAG TACGAAATGC TGTGCCGTGG GGAGGGTCTC 
AAAATGACTC CTCGGAGACA AAAAAGACTG TTCTGCCGCT ACTACGATGG AAACAGGAAT 
CCAAGATACA TTCTGGGCCC TGTCAAGCAG GAAGATGAGT GGGACAAGCC TCGAATTGTT 
CGCTTTCTTG ATATCATTTC CGATGAAGAG ATTGAAACTG TGAAAGAACT AGCAAAGCCA 
AGGCTGAGGC GAGCCACCAT TTCAAACCCC ATAACAGGAG CCTTGGAGAC GGCACATTAC 
AGAATTAGCA AAAGTGCTTG GTTGTCTGGA TATGAAAGCC CTGTGGTATC TCGCATTAAC 
ACCAGGATAC AGGACCTAAC AGGGCTCGAT GTCTCCACAG CAGAGGAACT TCAGGTAGCC 
AACTACGGCG TAGGAGGCCA GTATGAGCCT CATTTTGATT TCGGAAGGAA AGACGAGCCA 
GATGCTTTTA AAGAATTGGG AACAGGCAAC AGAATTGCTA CTTGGTTGTT TTATATGAGC 
GACGTGTCAG CAGGGGGAGC TACCGTCTTT CCTGAAGTAG GAGCAAGCGT TTGGCCTAAA 
AAAGGAACAG CTGTATTCTG GTACAATCTC TTCCCAAGTG GAGAAGGAGA TTACAGCACA 
CGGCATGCAG CCTGCCCAGT ACTAGTTGGA AACAAATGGG TGTCCAATAA ATGGCTCCAC 
GAGCGGGGAC AGGAATTCCG AAGGCCGTGC ACGTTATCAG AGTTGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cell density regulates prolyl 4-hydroxylase activity independent of mRNA levels.
Matrix biology : journal of the International Society for Matrix Biology19(8)779-82(2001 Jan)
Lee EH,Kao WW,Schwarz RI

Prolyl 4-hydroxylase: molecular cloning and the primary structure of the alpha subunit from chicken embryo.
Proceedings of the National Academy of Sciences of the United States of America86(19)7382-6(1989 Oct)
Bassuk JA,Kao WW,Herzer P,Kedersha NL,Seyer J,DeMartino JA,Daugherty BL,Mark GE 3rd,Berg RA

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P4HA1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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