MADD cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	MADD
Entrez Gene ID	423184
Full Name	MAP kinase activating death domain
General protein information	Preferred Names MAP kinase activating death domain Names MAP kinase-activating death domain protein
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015287154.1	XP_015142640.1	MAP kinase-activating death domain protein isoform X1
XM_015287160.1	XP_015142646.1	MAP kinase-activating death domain protein isoform X5
XM_015287163.1	XP_015142649.1	MAP kinase-activating death domain protein isoform X8
XM_015287166.2	XP_015142652.1	MAP kinase-activating death domain protein isoform X10
XM_015287177.2	XP_015142663.1	MAP kinase-activating death domain protein isoform X21
XM_015287184.2	XP_015142670.1	MAP kinase-activating death domain protein isoform X29
XM_015287155.1	XP_015142641.1	MAP kinase-activating death domain protein isoform X1
XM_015287157.1	XP_015142643.1	MAP kinase-activating death domain protein isoform X2
XM_015287170.1	XP_015142656.1	MAP kinase-activating death domain protein isoform X14
XM_015287175.1	XP_015142661.1	MAP kinase-activating death domain protein isoform X19
XM_015287178.1	XP_015142664.1	MAP kinase-activating death domain protein isoform X22
XM_015287162.2	XP_015142648.1	MAP kinase-activating death domain protein isoform X7
XM_015287169.2	XP_015142655.1	MAP kinase-activating death domain protein isoform X13
XM_015287175.2	XP_015142661.1	MAP kinase-activating death domain protein isoform X19
XM_015287183.2	XP_015142669.1	MAP kinase-activating death domain protein isoform X27
XM_015287168.1	XP_015142654.1	MAP kinase-activating death domain protein isoform X12
XM_015287172.1	XP_015142658.1	MAP kinase-activating death domain protein isoform X16
XM_015287174.1	XP_015142660.1	MAP kinase-activating death domain protein isoform X18
XM_015287177.1	XP_015142663.1	MAP kinase-activating death domain protein isoform X21
XM_015287184.1	XP_015142670.1	MAP kinase-activating death domain protein isoform X28
XM_015287159.2	XP_015142645.1	MAP kinase-activating death domain protein isoform X4
XM_015287160.2	XP_015142646.1	MAP kinase-activating death domain protein isoform X5
XM_015287167.2	XP_015142653.1	MAP kinase-activating death domain protein isoform X11
XM_015287174.2	XP_015142660.1	MAP kinase-activating death domain protein isoform X18
XM_015287178.2	XP_015142664.1	MAP kinase-activating death domain protein isoform X22
XM_015287180.2	XP_015142666.1	MAP kinase-activating death domain protein isoform X24
XM_015287156.1	XP_015142642.1	MAP kinase-activating death domain protein isoform X1
XM_015287166.1	XP_015142652.1	MAP kinase-activating death domain protein isoform X10
XM_015287167.1	XP_015142653.1	MAP kinase-activating death domain protein isoform X11
XM_015287171.1	XP_015142657.1	MAP kinase-activating death domain protein isoform X15
XM_015287176.1	XP_015142662.1	MAP kinase-activating death domain protein isoform X20
XM_015287156.2	XP_015142642.1	MAP kinase-activating death domain protein isoform X1
XM_015287157.2	XP_015142643.1	MAP kinase-activating death domain protein isoform X2
XM_015287165.2	XP_015142651.1	MAP kinase-activating death domain protein isoform X9
XM_015287168.2	XP_015142654.1	MAP kinase-activating death domain protein isoform X12
XM_015287171.2	XP_015142657.1	MAP kinase-activating death domain protein isoform X15
XM_015287181.2	XP_015142667.1	MAP kinase-activating death domain protein isoform X25
XM_015287182.2	XP_015142668.1	MAP kinase-activating death domain protein isoform X26
XM_015287158.1	XP_015142644.1	MAP kinase-activating death domain protein isoform X3
XM_015287159.1	XP_015142645.1	MAP kinase-activating death domain protein isoform X4
XM_015287161.1	XP_015142647.1	MAP kinase-activating death domain protein isoform X6
XM_015287162.1	XP_015142648.1	MAP kinase-activating death domain protein isoform X7
XM_015287165.1	XP_015142651.1	MAP kinase-activating death domain protein isoform X9
XM_015287169.1	XP_015142655.1	MAP kinase-activating death domain protein isoform X13
XM_015287173.1	XP_015142659.1	MAP kinase-activating death domain protein isoform X17
XM_015287179.1	XP_015142665.1	MAP kinase-activating death domain protein isoform X23
XM_015287180.1	XP_015142666.1	MAP kinase-activating death domain protein isoform X24
XM_015287181.1	XP_015142667.1	MAP kinase-activating death domain protein isoform X25
XM_015287182.1	XP_015142668.1	MAP kinase-activating death domain protein isoform X26
XM_015287183.1	XP_015142669.1	MAP kinase-activating death domain protein isoform X27
XM_015287154.2	XP_015142640.1	MAP kinase-activating death domain protein isoform X1
XM_015287155.2	XP_015142641.1	MAP kinase-activating death domain protein isoform X1
XM_015287158.2	XP_015142644.1	MAP kinase-activating death domain protein isoform X3
XM_015287161.2	XP_015142647.1	MAP kinase-activating death domain protein isoform X6
XM_015287163.2	XP_015142649.1	MAP kinase-activating death domain protein isoform X8
XM_015287170.2	XP_015142656.1	MAP kinase-activating death domain protein isoform X14
XM_015287172.2	XP_015142658.1	MAP kinase-activating death domain protein isoform X16
XM_015287173.2	XP_015142659.1	MAP kinase-activating death domain protein isoform X17
XM_015287176.2	XP_015142662.1	MAP kinase-activating death domain protein isoform X20
XM_015287179.2	XP_015142665.1	MAP kinase-activating death domain protein isoform X23
XM_025151374.1	XP_025007142.1	MAP kinase-activating death domain protein isoform X28

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MADD	NP_001180007.1
Rattus norvegicus (Norway rat)	Madd	NP_446037.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000630	XP_311160.5
Caenorhabditis elegans (roundworm)	aex-3	NP_741710.1
Xenopus tropicalis (tropical clawed frog)	madd	NP_001072717.1
Mus musculus (house mouse)	Madd	NP_001171191.1
Danio rerio (zebrafish)	madd	XP_005169012.1
	MADD	XP_001107689.1
Homo sapiens (human)	MADD	NP_003673.3
Pan troglodytes (chimpanzee)	MADD	XP_003951918.1
Canis lupus familiaris (dog)	MADD	XP_005631300.1
Gallus gallus (chicken)	MADD	XP_004941623.1
Drosophila melanogaster (fruit fly)	rab3-GEF	NP_001096983.2

Gene Ontology

The following MADD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MADD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa151373	XM_025151374.1 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OGa36674	XM_015287154.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa36674	XM_015287155.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa36674	XM_015287156.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa36675	XM_015287157.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa36676	XM_015287158.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa36677	XM_015287160.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa36678	XM_015287161.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa36679	XM_015287162.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa36680	XM_015287163.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa36681	XM_015287165.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa36682	XM_015287166.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa36683	XM_015287170.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa36684	XM_015287173.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa36685	XM_015287174.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa36686	XM_015287175.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa36687	XM_015287178.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa09157	XM_015287159.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa09162	XM_015287168.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa09163	XM_015287167.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa09165	XM_015287169.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa09166	XM_015287171.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa09167	XM_015287172.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa09168	XM_015287177.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa09169	XM_015287176.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa09171	XM_015287179.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa09172	XM_015287180.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa09174	XM_015287181.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa09175	XM_015287182.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa09176	XM_015287183.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa09177	XM_015287184.2 Latest version!	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OGa36686	XM_015287175.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa09168	XM_015287177.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa09177	XM_015287184.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa36677	XM_015287160.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa36685	XM_015287174.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa36687	XM_015287178.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa36682	XM_015287166.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa09163	XM_015287167.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa36674	XM_015287156.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa36675	XM_015287157.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa09162	XM_015287168.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa09166	XM_015287171.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa09157	XM_015287159.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa36679	XM_015287162.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa36681	XM_015287165.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa09165	XM_015287169.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa09172	XM_015287180.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa09174	XM_015287181.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa09175	XM_015287182.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa09176	XM_015287183.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa36674	XM_015287154.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa36674	XM_015287155.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa36676	XM_015287158.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa36678	XM_015287161.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa36680	XM_015287163.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa36683	XM_015287170.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa09167	XM_015287172.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa36684	XM_015287173.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa09169	XM_015287176.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa09171	XM_015287179.1	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MADD ( XM_025151374.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025007142.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X30, mRNA.

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CloneID	OGa151373
Clone ID Related Accession (Same CDS sequence)	XM_025151374.1
Accession Version	XM_025151374.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG TAGGGATAAA GGATCTGTCT GGGACCAGTT AGAGGATGCC 
GCAATGGAAA CCTTCTCTAT GAGCAAAGAG CGCTCAACAC TGTGGGACCA GATGCAATTC 
TGGGAAGATG CCTTTTTGGA TGCTGTGATG TTAGAGAGAG AAGGAATGGG GATGGATCAG 
GGACCCCAGG AGATGATTGA CAGGTATCTT TCCCTGGGAG AACATGATCG AAAACGTTTG 
GAGGATGATG AGGACCGCTT ATTGGCTACG CTGCTACATA ATATGATTGC TTATATGCTT 
ATGATAAAGG TGAATAAGAA TGATATTAGG AAAAAGGTGC GTCGTCTGAT GGGAAAATCG 
CATATTGGAT TGTTGCACAG CCAGCAGTTA AATGACATCC TAGACAAGCT TGCTAACCTG 
AATGGACGGG AACTTCCTGT GAGACCCAGC GGCAGCCGCC ACATCAAGAA GCAGACTTTT 
GTAGTCCATG CTGGGACAGA CACTACAGGA GATATATTTT TCATGGAGGT TTGTGATGAC 
TGCATTGTGC TGAGAAGCAA CATTGGAACT GTGTATGAAC GTTGGTGGTA TGAGAAACTC 
ATCAACATGA CTTACTGTCC CAAAACAAAA GTTCTGTGTC TCTGGCGGAG GAATGGTCAG 
GAAACACAAC TGAACAAGTT CTACACAAAG AAGTGTCGGG AATTATACTA CTGCGTAAAA 
GACAGCATGG AGCGAGCAGC AGCAAGACAG CAAAGCATTA AGCCAGGCCC TGAGTTGGGT 
GGTGAGTTTC CTGTGCAGGA TATGAAAACT GGCGAAGGAG GTCTTCTGCA GGTGACACTG 
GAAGGAATTA ACCTGAAATT TATGCACAGT CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CTGTGCGAGG AGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AACTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACGCCAATG GCTCACGAGA TCTGCTACTC CGTGCTGTGT 
CTCTTCTCTT ATGTGGCTGC CATTCGTGGG AAAGAGGCAG AAAACAAAAG CAAACCACCT 
CGGCCAGTTT CAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025007142.1
CDS	277..5154
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIGPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_025151374.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025151374.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG TAGGGATAAA GGATCTGTCT GGGACCAGTT AGAGGATGCC 
GCAATGGAAA CCTTCTCTAT GAGCAAAGAG CGCTCAACAC TGTGGGACCA GATGCAATTC 
TGGGAAGATG CCTTTTTGGA TGCTGTGATG TTAGAGAGAG AAGGAATGGG GATGGATCAG 
GGACCCCAGG AGATGATTGA CAGGTATCTT TCCCTGGGAG AACATGATCG AAAACGTTTG 
GAGGATGATG AGGACCGCTT ATTGGCTACG CTGCTACATA ATATGATTGC TTATATGCTT 
ATGATAAAGG TGAATAAGAA TGATATTAGG AAAAAGGTGC GTCGTCTGAT GGGAAAATCG 
CATATTGGAT TGTTGCACAG CCAGCAGTTA AATGACATCC TAGACAAGCT TGCTAACCTG 
AATGGACGGG AACTTCCTGT GAGACCCAGC GGCAGCCGCC ACATCAAGAA GCAGACTTTT 
GTAGTCCATG CTGGGACAGA CACTACAGGA GATATATTTT TCATGGAGGT TTGTGATGAC 
TGCATTGTGC TGAGAAGCAA CATTGGAACT GTGTATGAAC GTTGGTGGTA TGAGAAACTC 
ATCAACATGA CTTACTGTCC CAAAACAAAA GTTCTGTGTC TCTGGCGGAG GAATGGTCAG 
GAAACACAAC TGAACAAGTT CTACACAAAG AAGTGTCGGG AATTATACTA CTGCGTAAAA 
GACAGCATGG AGCGAGCAGC AGCAAGACAG CAAAGCATTA AGCCAGGCCC TGAGTTGGGT 
GGTGAGTTTC CTGTGCAGGA TATGAAAACT GGCGAAGGAG GTCTTCTGCA GGTGACACTG 
GAAGGAATTA ACCTGAAATT TATGCACAGT CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CTGTGCGAGG AGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AACTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACGCCAATG GCTCACGAGA TCTGCTACTC CGTGCTGTGT 
CTCTTCTCTT ATGTGGCTGC CATTCGTGGG AAAGAGGCAG AAAACAAAAG CAAACCACCT 
CGGCCAGTTT CAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287154.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142640.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X1, mRNA.

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CloneID	OGa36674
Clone ID Related Accession (Same CDS sequence)	XM_015287154.1 , XM_015287155.1 , XM_015287156.1 , XM_015287156.2 , XM_015287154.2 , XM_015287155.2
Accession Version	XM_015287154.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACTGTGCGA 
GGAGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAGTAC 
AAGACGCCAA TGGCTCACGA GATCTGCTAC TCCGTGCTGT GTCTCTTCTC TTATGTGGCT 
GCCATTCGTG GGAAAGAGGC AGAAAACAAA AGCAAACCAC CTCGGCCAGT TTCAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142640.1
CDS	233..5332
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKDYFNLEVGRDVNEVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287154.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287154.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACTGTGCGA 
GGAGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAGTAC 
AAGACGCCAA TGGCTCACGA GATCTGCTAC TCCGTGCTGT GTCTCTTCTC TTATGTGGCT 
GCCATTCGTG GGAAAGAGGC AGAAAACAAA AGCAAACCAC CTCGGCCAGT TTCAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287155.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142641.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X2, mRNA.

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CloneID	OGa36674
Clone ID Related Accession (Same CDS sequence)	XM_015287154.1 , XM_015287155.1 , XM_015287156.1 , XM_015287156.2 , XM_015287154.2 , XM_015287155.2
Accession Version	XM_015287155.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACTGTGCGA 
GGAGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAGTAC 
AAGACGCCAA TGGCTCACGA GATCTGCTAC TCCGTGCTGT GTCTCTTCTC TTATGTGGCT 
GCCATTCGTG GGAAAGAGGC AGAAAACAAA AGCAAACCAC CTCGGCCAGT TTCAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142641.1
CDS	234..5333
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKDYFNLEVGRDVNEVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287155.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287155.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACTGTGCGA 
GGAGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAGTAC 
AAGACGCCAA TGGCTCACGA GATCTGCTAC TCCGTGCTGT GTCTCTTCTC TTATGTGGCT 
GCCATTCGTG GGAAAGAGGC AGAAAACAAA AGCAAACCAC CTCGGCCAGT TTCAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287156.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142642.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X3, mRNA.

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CloneID	OGa36674
Clone ID Related Accession (Same CDS sequence)	XM_015287154.1 , XM_015287155.1 , XM_015287156.1 , XM_015287156.2 , XM_015287154.2 , XM_015287155.2
Accession Version	XM_015287156.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACTGTGCGA 
GGAGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAGTAC 
AAGACGCCAA TGGCTCACGA GATCTGCTAC TCCGTGCTGT GTCTCTTCTC TTATGTGGCT 
GCCATTCGTG GGAAAGAGGC AGAAAACAAA AGCAAACCAC CTCGGCCAGT TTCAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142642.1
CDS	445..5544
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKDYFNLEVGRDVNEVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287156.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287156.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACTGTGCGA 
GGAGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAGTAC 
AAGACGCCAA TGGCTCACGA GATCTGCTAC TCCGTGCTGT GTCTCTTCTC TTATGTGGCT 
GCCATTCGTG GGAAAGAGGC AGAAAACAAA AGCAAACCAC CTCGGCCAGT TTCAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287156.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142642.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X3, mRNA.

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CloneID	OGa36674
Clone ID Related Accession (Same CDS sequence)	XM_015287154.1 , XM_015287155.1 , XM_015287156.1 , XM_015287156.2 , XM_015287154.2 , XM_015287155.2
Accession Version	XM_015287156.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287156.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACTGTGCGA 
GGAGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAGTAC 
AAGACGCCAA TGGCTCACGA GATCTGCTAC TCCGTGCTGT GTCTCTTCTC TTATGTGGCT 
GCCATTCGTG GGAAAGAGGC AGAAAACAAA AGCAAACCAC CTCGGCCAGT TTCAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142642.1
CDS	430..5529
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKDYFNLEVGRDVNEVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287156.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287156.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACTGTGCGA 
GGAGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAGTAC 
AAGACGCCAA TGGCTCACGA GATCTGCTAC TCCGTGCTGT GTCTCTTCTC TTATGTGGCT 
GCCATTCGTG GGAAAGAGGC AGAAAACAAA AGCAAACCAC CTCGGCCAGT TTCAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287154.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142640.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa36674
Clone ID Related Accession (Same CDS sequence)	XM_015287154.1 , XM_015287155.1 , XM_015287156.1 , XM_015287156.2 , XM_015287154.2 , XM_015287155.2
Accession Version	XM_015287154.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287154.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACTGTGCGA 
GGAGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAGTAC 
AAGACGCCAA TGGCTCACGA GATCTGCTAC TCCGTGCTGT GTCTCTTCTC TTATGTGGCT 
GCCATTCGTG GGAAAGAGGC AGAAAACAAA AGCAAACCAC CTCGGCCAGT TTCAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142640.1
CDS	277..5376
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKDYFNLEVGRDVNEVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287154.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287154.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACTGTGCGA 
GGAGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAGTAC 
AAGACGCCAA TGGCTCACGA GATCTGCTAC TCCGTGCTGT GTCTCTTCTC TTATGTGGCT 
GCCATTCGTG GGAAAGAGGC AGAAAACAAA AGCAAACCAC CTCGGCCAGT TTCAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287155.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142641.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa36674
Clone ID Related Accession (Same CDS sequence)	XM_015287154.1 , XM_015287155.1 , XM_015287156.1 , XM_015287156.2 , XM_015287154.2 , XM_015287155.2
Accession Version	XM_015287155.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287155.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACTGTGCGA 
GGAGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAGTAC 
AAGACGCCAA TGGCTCACGA GATCTGCTAC TCCGTGCTGT GTCTCTTCTC TTATGTGGCT 
GCCATTCGTG GGAAAGAGGC AGAAAACAAA AGCAAACCAC CTCGGCCAGT TTCAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142641.1
CDS	249..5348
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKDYFNLEVGRDVNEVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287155.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287155.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACTGTGCGA 
GGAGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAGTAC 
AAGACGCCAA TGGCTCACGA GATCTGCTAC TCCGTGCTGT GTCTCTTCTC TTATGTGGCT 
GCCATTCGTG GGAAAGAGGC AGAAAACAAA AGCAAACCAC CTCGGCCAGT TTCAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287157.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142643.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X4, mRNA.

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CloneID	OGa36675
Clone ID Related Accession (Same CDS sequence)	XM_015287157.1 , XM_015287157.2
Accession Version	XM_015287157.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGTGG CAGTGACAGT GTCGACTATG ATGACTCAAG TTCCTCCTAC 
TCTTCCCTTG GAGACTTTGT CAGTGAGATG ATGAAATGTG ACATTAATGG CGATACTCCA 
AATGTTGACC CCCTGACTCA CGCAGCCCTT GGTGATGCCA GTGAAGTGGA GTTCGATGAT 
TTTCAAGAAT ATTCAGGAGA GCTGGATGAA CAGGCCATGG ACAGCGAGAA CTCCCAAGAG 
AACAATCAGC CTCGTTCAAG TTCCAGTACT ACAGCCAGTA GCAGCCCCAG TACCGTCATC 
CATGGAGTGA ATCATGAGTC TGCAGATTCA GCAGAGATGG AGGAGAAGCT AGTTGCCGGA 
TTTTCAAACC ATCTCCCTTC TTTGCCACTG CAACCAAGCT TTCCCAAGAT GAGCTTTGAT 
CGTCGTGAGA GTGATAGCCC AGCTGTCAGC ATGAGCTCCT CAGAAGGGGT GGTGAGGAAA 
CGAGAGTATG ATAATCCATA CTTCGAACCA CAGTATGGTT TTCCTACAGA GGATGAAGAT 
GATGAGCAGG AAGAGAGCTA CACCCCAAGA TTTAACCAGA ATCTCAATGG AAGCAGGTCT 
CAGAAGTTAC TTCGTCCAAA CAGTTTAAAA CTGGCCAATG ATTCTGATGC AGATTCAGAC 
TCCAGGGCCA GCTCTCCAAA CTCTACTGTC TCCAACAACA GCAGTGAAGG TTTTGGGGGC 
ATCATGTCTT TTGCAAGCAG CCTGTACAGA AATCACAGCA CAAGTTTCAG TCTGTCCAAT 
TTAGCCCTGC CAACCAAAGT TGGGAGAGAC AAGAATACCC CCTTTCCCAG CCTGAAAGAT 
TACTTTAATC TGGAAGTGGG AAGGGATGTG AATGAAGTAT TTGGGTTAAA TACTATAATG 
GAGATTATTA CTGAAGCTGG CCCAGTAAGC AATGAAGGAA ACAGGAGAGC CCTGGTGGAT 
CAAAAGTCTT CAGTCATTAA GCATAGCCCA ACAGTGAAGA GGGAATCTCC ATCACCTCAG 
GGACGAAGTA GTAACTCCAG TGAGAACCAG CAATTCCTGA AGGAGGTGGT ACACAATGTT 
CTCGATGGGC AAGGTGTTGG TTGGCTGAAC ATGAAGAGAG TCCGACGTCT GCTGGAGAGT 
GAGCAGCTCC GTGTCTTTGT ACTAAGCAAG CTAAATCGCA CGATCCAGTC GGAAGAAGAT 
GCTCAGCAGG ATATCATACA GGATGTGGAG ATCAGCCGCA AAGTTTATAA AGGCATGCTG 
GACTTGCTAA AGTGTACTGT GCTGAGCCTG GAGCAGTCAT ACGCAAATGC TGGCCTGGGA 
GGCATGGCCA GTGTTTTTGG CCTTCTAGAA ATAGCACATA CTCACTATTA CAACAAAGAA 
CCAGAAAAGA GAAAACGAAG CCCAACAGAT GGATCTGTCA CTCCGGTTGG CAAAGATCCT 
GCATCATCTC CAAGAGTGGA GCCGAAACCT GCCATGCAGC TGCCAGTACC ACAGCTGATG 
CCGAAGGCAC CAAGCCCTGC AGGGAAAGGG CTAAGGGAGT TTGATACAAG GAGTCTAAAG 
GAAGAAAATT TTATTGCTTC CATTGAATTG TGGAACAAGC ACCAGGAAGT GAAAAAGCAA 
AAATCTTTGG AAAAAACGAG GCCAGAAGGT GTGAAACATC TTGATTTGGG AGAAACAGAT 
GAGAAAAAAT CCCAAATCAG TGCAGACAGT GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG 
AGTGATTTTG ACTCTGTTCC CAGTGGAGGA CCGTCAGTTA TGGTCCGAAG TACCAGCCAG 
GATTCTGAAG TTAGCACAGT GGTTAGTAAC AGCTCTGGGG AGACACTAGG AGCAGACAGT 
GACTTGAGTA GCAATGCTGG TGATGGCCCA AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA 
TCCAGGGGCA CCGTGTCAGA CAGTGAAATT GAGACAAACT CTGCTACTAG CTCTATTTTC 
GCAAAGTCTC ATAACTTGAA GCAGAATGTA AAGGAGAACA AAGGCAGTAC TGCAGGGAAA 
GGTCCAGAGG ATGGGAACCA GCGTGTCTAT CTCTATGAAG GACTTTTGGG TAGGGATAAA 
GGATCTGTCT GGGACCAGTT AGAGGATGCC GCAATGGAAA CCTTCTCTAT GAGCAAAGAG 
CGCTCAACAC TGTGGGACCA GATGCAATTC TGGGAAGATG CCTTTTTGGA TGCTGTGATG 
TTAGAGAGAG AAGGAATGGG GATGGATCAG GGACCCCAGG AGATGATTGA CAGGTATCTT 
TCCCTGGGAG AACATGATCG AAAACGTTTG GAGGATGATG AGGACCGCTT ATTGGCTACG 
CTGCTACATA ATATGATTGC TTATATGCTT ATGATAAAGG TGAATAAGAA TGATATTAGG 
AAAAAGGTGC GTCGTCTGAT GGGAAAATCG CATATTGGAT TGTTGCACAG CCAGCAGTTA 
AATGACATCC TAGACAAGCT TGCTAACCTG AATGGACGGG AACTTCCTGT GAGACCCAGC 
GGCAGCCGCC ACATCAAGAA GCAGACTTTT GTAGTCCATG CTGGGACAGA CACTACAGGA 
GATATATTTT TCATGGAGGT TTGTGATGAC TGCATTGTGC TGAGAAGCAA CATTGGAACT 
GTGTATGAAC GTTGGTGGTA TGAGAAACTC ATCAACATGA CTTACTGTCC CAAAACAAAA 
GTTCTGTGTC TCTGGCGGAG GAATGGTCAG GAAACACAAC TGAACAAGTT CTACACAAAG 
AAGTGTCGGG AATTATACTA CTGCGTAAAA GACAGCATGG AGCGAGCAGC AGCAAGACAG 
CAAAGCATTA AGCCAGGCCC TGAGTTGGGT GGTGAGTTTC CTGTGCAGGA TATGAAAACT 
GGCGAAGGAG GTCTTCTGCA GGTGACACTG GAAGGAATTA ACCTGAAATT TATGCACAGT 
CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT CACATTAAAA AGTGCAATAC TGTGCGAGGA 
GTCTTTGTCC TGGAGGAATT TGTTCCTGAA ACTAAAGAAG TGGTGAGCCA CAAGTACAAG 
ACGCCAATGG CTCACGAGAT CTGCTACTCC GTGCTGTGTC TCTTCTCTTA TGTGGCTGCC 
ATTCGTGGGA AAGAGGCAGA AAACAAAAGC AAACCACCTC GGCCAGTTTC AAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142643.1
CDS	233..5329
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKDYFNLEVGRDVNEVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287157.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287157.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGTGG CAGTGACAGT GTCGACTATG ATGACTCAAG TTCCTCCTAC 
TCTTCCCTTG GAGACTTTGT CAGTGAGATG ATGAAATGTG ACATTAATGG CGATACTCCA 
AATGTTGACC CCCTGACTCA CGCAGCCCTT GGTGATGCCA GTGAAGTGGA GTTCGATGAT 
TTTCAAGAAT ATTCAGGAGA GCTGGATGAA CAGGCCATGG ACAGCGAGAA CTCCCAAGAG 
AACAATCAGC CTCGTTCAAG TTCCAGTACT ACAGCCAGTA GCAGCCCCAG TACCGTCATC 
CATGGAGTGA ATCATGAGTC TGCAGATTCA GCAGAGATGG AGGAGAAGCT AGTTGCCGGA 
TTTTCAAACC ATCTCCCTTC TTTGCCACTG CAACCAAGCT TTCCCAAGAT GAGCTTTGAT 
CGTCGTGAGA GTGATAGCCC AGCTGTCAGC ATGAGCTCCT CAGAAGGGGT GGTGAGGAAA 
CGAGAGTATG ATAATCCATA CTTCGAACCA CAGTATGGTT TTCCTACAGA GGATGAAGAT 
GATGAGCAGG AAGAGAGCTA CACCCCAAGA TTTAACCAGA ATCTCAATGG AAGCAGGTCT 
CAGAAGTTAC TTCGTCCAAA CAGTTTAAAA CTGGCCAATG ATTCTGATGC AGATTCAGAC 
TCCAGGGCCA GCTCTCCAAA CTCTACTGTC TCCAACAACA GCAGTGAAGG TTTTGGGGGC 
ATCATGTCTT TTGCAAGCAG CCTGTACAGA AATCACAGCA CAAGTTTCAG TCTGTCCAAT 
TTAGCCCTGC CAACCAAAGT TGGGAGAGAC AAGAATACCC CCTTTCCCAG CCTGAAAGAT 
TACTTTAATC TGGAAGTGGG AAGGGATGTG AATGAAGTAT TTGGGTTAAA TACTATAATG 
GAGATTATTA CTGAAGCTGG CCCAGTAAGC AATGAAGGAA ACAGGAGAGC CCTGGTGGAT 
CAAAAGTCTT CAGTCATTAA GCATAGCCCA ACAGTGAAGA GGGAATCTCC ATCACCTCAG 
GGACGAAGTA GTAACTCCAG TGAGAACCAG CAATTCCTGA AGGAGGTGGT ACACAATGTT 
CTCGATGGGC AAGGTGTTGG TTGGCTGAAC ATGAAGAGAG TCCGACGTCT GCTGGAGAGT 
GAGCAGCTCC GTGTCTTTGT ACTAAGCAAG CTAAATCGCA CGATCCAGTC GGAAGAAGAT 
GCTCAGCAGG ATATCATACA GGATGTGGAG ATCAGCCGCA AAGTTTATAA AGGCATGCTG 
GACTTGCTAA AGTGTACTGT GCTGAGCCTG GAGCAGTCAT ACGCAAATGC TGGCCTGGGA 
GGCATGGCCA GTGTTTTTGG CCTTCTAGAA ATAGCACATA CTCACTATTA CAACAAAGAA 
CCAGAAAAGA GAAAACGAAG CCCAACAGAT GGATCTGTCA CTCCGGTTGG CAAAGATCCT 
GCATCATCTC CAAGAGTGGA GCCGAAACCT GCCATGCAGC TGCCAGTACC ACAGCTGATG 
CCGAAGGCAC CAAGCCCTGC AGGGAAAGGG CTAAGGGAGT TTGATACAAG GAGTCTAAAG 
GAAGAAAATT TTATTGCTTC CATTGAATTG TGGAACAAGC ACCAGGAAGT GAAAAAGCAA 
AAATCTTTGG AAAAAACGAG GCCAGAAGGT GTGAAACATC TTGATTTGGG AGAAACAGAT 
GAGAAAAAAT CCCAAATCAG TGCAGACAGT GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG 
AGTGATTTTG ACTCTGTTCC CAGTGGAGGA CCGTCAGTTA TGGTCCGAAG TACCAGCCAG 
GATTCTGAAG TTAGCACAGT GGTTAGTAAC AGCTCTGGGG AGACACTAGG AGCAGACAGT 
GACTTGAGTA GCAATGCTGG TGATGGCCCA AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA 
TCCAGGGGCA CCGTGTCAGA CAGTGAAATT GAGACAAACT CTGCTACTAG CTCTATTTTC 
GCAAAGTCTC ATAACTTGAA GCAGAATGTA AAGGAGAACA AAGGCAGTAC TGCAGGGAAA 
GGTCCAGAGG ATGGGAACCA GCGTGTCTAT CTCTATGAAG GACTTTTGGG TAGGGATAAA 
GGATCTGTCT GGGACCAGTT AGAGGATGCC GCAATGGAAA CCTTCTCTAT GAGCAAAGAG 
CGCTCAACAC TGTGGGACCA GATGCAATTC TGGGAAGATG CCTTTTTGGA TGCTGTGATG 
TTAGAGAGAG AAGGAATGGG GATGGATCAG GGACCCCAGG AGATGATTGA CAGGTATCTT 
TCCCTGGGAG AACATGATCG AAAACGTTTG GAGGATGATG AGGACCGCTT ATTGGCTACG 
CTGCTACATA ATATGATTGC TTATATGCTT ATGATAAAGG TGAATAAGAA TGATATTAGG 
AAAAAGGTGC GTCGTCTGAT GGGAAAATCG CATATTGGAT TGTTGCACAG CCAGCAGTTA 
AATGACATCC TAGACAAGCT TGCTAACCTG AATGGACGGG AACTTCCTGT GAGACCCAGC 
GGCAGCCGCC ACATCAAGAA GCAGACTTTT GTAGTCCATG CTGGGACAGA CACTACAGGA 
GATATATTTT TCATGGAGGT TTGTGATGAC TGCATTGTGC TGAGAAGCAA CATTGGAACT 
GTGTATGAAC GTTGGTGGTA TGAGAAACTC ATCAACATGA CTTACTGTCC CAAAACAAAA 
GTTCTGTGTC TCTGGCGGAG GAATGGTCAG GAAACACAAC TGAACAAGTT CTACACAAAG 
AAGTGTCGGG AATTATACTA CTGCGTAAAA GACAGCATGG AGCGAGCAGC AGCAAGACAG 
CAAAGCATTA AGCCAGGCCC TGAGTTGGGT GGTGAGTTTC CTGTGCAGGA TATGAAAACT 
GGCGAAGGAG GTCTTCTGCA GGTGACACTG GAAGGAATTA ACCTGAAATT TATGCACAGT 
CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT CACATTAAAA AGTGCAATAC TGTGCGAGGA 
GTCTTTGTCC TGGAGGAATT TGTTCCTGAA ACTAAAGAAG TGGTGAGCCA CAAGTACAAG 
ACGCCAATGG CTCACGAGAT CTGCTACTCC GTGCTGTGTC TCTTCTCTTA TGTGGCTGCC 
ATTCGTGGGA AAGAGGCAGA AAACAAAAGC AAACCACCTC GGCCAGTTTC AAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287157.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142643.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X4, mRNA.

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CloneID	OGa36675
Clone ID Related Accession (Same CDS sequence)	XM_015287157.1 , XM_015287157.2
Accession Version	XM_015287157.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287157.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGTGG CAGTGACAGT GTCGACTATG ATGACTCAAG TTCCTCCTAC 
TCTTCCCTTG GAGACTTTGT CAGTGAGATG ATGAAATGTG ACATTAATGG CGATACTCCA 
AATGTTGACC CCCTGACTCA CGCAGCCCTT GGTGATGCCA GTGAAGTGGA GTTCGATGAT 
TTTCAAGAAT ATTCAGGAGA GCTGGATGAA CAGGCCATGG ACAGCGAGAA CTCCCAAGAG 
AACAATCAGC CTCGTTCAAG TTCCAGTACT ACAGCCAGTA GCAGCCCCAG TACCGTCATC 
CATGGAGTGA ATCATGAGTC TGCAGATTCA GCAGAGATGG AGGAGAAGCT AGTTGCCGGA 
TTTTCAAACC ATCTCCCTTC TTTGCCACTG CAACCAAGCT TTCCCAAGAT GAGCTTTGAT 
CGTCGTGAGA GTGATAGCCC AGCTGTCAGC ATGAGCTCCT CAGAAGGGGT GGTGAGGAAA 
CGAGAGTATG ATAATCCATA CTTCGAACCA CAGTATGGTT TTCCTACAGA GGATGAAGAT 
GATGAGCAGG AAGAGAGCTA CACCCCAAGA TTTAACCAGA ATCTCAATGG AAGCAGGTCT 
CAGAAGTTAC TTCGTCCAAA CAGTTTAAAA CTGGCCAATG ATTCTGATGC AGATTCAGAC 
TCCAGGGCCA GCTCTCCAAA CTCTACTGTC TCCAACAACA GCAGTGAAGG TTTTGGGGGC 
ATCATGTCTT TTGCAAGCAG CCTGTACAGA AATCACAGCA CAAGTTTCAG TCTGTCCAAT 
TTAGCCCTGC CAACCAAAGT TGGGAGAGAC AAGAATACCC CCTTTCCCAG CCTGAAAGAT 
TACTTTAATC TGGAAGTGGG AAGGGATGTG AATGAAGTAT TTGGGTTAAA TACTATAATG 
GAGATTATTA CTGAAGCTGG CCCAGTAAGC AATGAAGGAA ACAGGAGAGC CCTGGTGGAT 
CAAAAGTCTT CAGTCATTAA GCATAGCCCA ACAGTGAAGA GGGAATCTCC ATCACCTCAG 
GGACGAAGTA GTAACTCCAG TGAGAACCAG CAATTCCTGA AGGAGGTGGT ACACAATGTT 
CTCGATGGGC AAGGTGTTGG TTGGCTGAAC ATGAAGAGAG TCCGACGTCT GCTGGAGAGT 
GAGCAGCTCC GTGTCTTTGT ACTAAGCAAG CTAAATCGCA CGATCCAGTC GGAAGAAGAT 
GCTCAGCAGG ATATCATACA GGATGTGGAG ATCAGCCGCA AAGTTTATAA AGGCATGCTG 
GACTTGCTAA AGTGTACTGT GCTGAGCCTG GAGCAGTCAT ACGCAAATGC TGGCCTGGGA 
GGCATGGCCA GTGTTTTTGG CCTTCTAGAA ATAGCACATA CTCACTATTA CAACAAAGAA 
CCAGAAAAGA GAAAACGAAG CCCAACAGAT GGATCTGTCA CTCCGGTTGG CAAAGATCCT 
GCATCATCTC CAAGAGTGGA GCCGAAACCT GCCATGCAGC TGCCAGTACC ACAGCTGATG 
CCGAAGGCAC CAAGCCCTGC AGGGAAAGGG CTAAGGGAGT TTGATACAAG GAGTCTAAAG 
GAAGAAAATT TTATTGCTTC CATTGAATTG TGGAACAAGC ACCAGGAAGT GAAAAAGCAA 
AAATCTTTGG AAAAAACGAG GCCAGAAGGT GTGAAACATC TTGATTTGGG AGAAACAGAT 
GAGAAAAAAT CCCAAATCAG TGCAGACAGT GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG 
AGTGATTTTG ACTCTGTTCC CAGTGGAGGA CCGTCAGTTA TGGTCCGAAG TACCAGCCAG 
GATTCTGAAG TTAGCACAGT GGTTAGTAAC AGCTCTGGGG AGACACTAGG AGCAGACAGT 
GACTTGAGTA GCAATGCTGG TGATGGCCCA AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA 
TCCAGGGGCA CCGTGTCAGA CAGTGAAATT GAGACAAACT CTGCTACTAG CTCTATTTTC 
GCAAAGTCTC ATAACTTGAA GCAGAATGTA AAGGAGAACA AAGGCAGTAC TGCAGGGAAA 
GGTCCAGAGG ATGGGAACCA GCGTGTCTAT CTCTATGAAG GACTTTTGGG TAGGGATAAA 
GGATCTGTCT GGGACCAGTT AGAGGATGCC GCAATGGAAA CCTTCTCTAT GAGCAAAGAG 
CGCTCAACAC TGTGGGACCA GATGCAATTC TGGGAAGATG CCTTTTTGGA TGCTGTGATG 
TTAGAGAGAG AAGGAATGGG GATGGATCAG GGACCCCAGG AGATGATTGA CAGGTATCTT 
TCCCTGGGAG AACATGATCG AAAACGTTTG GAGGATGATG AGGACCGCTT ATTGGCTACG 
CTGCTACATA ATATGATTGC TTATATGCTT ATGATAAAGG TGAATAAGAA TGATATTAGG 
AAAAAGGTGC GTCGTCTGAT GGGAAAATCG CATATTGGAT TGTTGCACAG CCAGCAGTTA 
AATGACATCC TAGACAAGCT TGCTAACCTG AATGGACGGG AACTTCCTGT GAGACCCAGC 
GGCAGCCGCC ACATCAAGAA GCAGACTTTT GTAGTCCATG CTGGGACAGA CACTACAGGA 
GATATATTTT TCATGGAGGT TTGTGATGAC TGCATTGTGC TGAGAAGCAA CATTGGAACT 
GTGTATGAAC GTTGGTGGTA TGAGAAACTC ATCAACATGA CTTACTGTCC CAAAACAAAA 
GTTCTGTGTC TCTGGCGGAG GAATGGTCAG GAAACACAAC TGAACAAGTT CTACACAAAG 
AAGTGTCGGG AATTATACTA CTGCGTAAAA GACAGCATGG AGCGAGCAGC AGCAAGACAG 
CAAAGCATTA AGCCAGGCCC TGAGTTGGGT GGTGAGTTTC CTGTGCAGGA TATGAAAACT 
GGCGAAGGAG GTCTTCTGCA GGTGACACTG GAAGGAATTA ACCTGAAATT TATGCACAGT 
CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT CACATTAAAA AGTGCAATAC TGTGCGAGGA 
GTCTTTGTCC TGGAGGAATT TGTTCCTGAA ACTAAAGAAG TGGTGAGCCA CAAGTACAAG 
ACGCCAATGG CTCACGAGAT CTGCTACTCC GTGCTGTGTC TCTTCTCTTA TGTGGCTGCC 
ATTCGTGGGA AAGAGGCAGA AAACAAAAGC AAACCACCTC GGCCAGTTTC AAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142643.1
CDS	277..5373
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKDYFNLEVGRDVNEVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287157.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287157.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGTGG CAGTGACAGT GTCGACTATG ATGACTCAAG TTCCTCCTAC 
TCTTCCCTTG GAGACTTTGT CAGTGAGATG ATGAAATGTG ACATTAATGG CGATACTCCA 
AATGTTGACC CCCTGACTCA CGCAGCCCTT GGTGATGCCA GTGAAGTGGA GTTCGATGAT 
TTTCAAGAAT ATTCAGGAGA GCTGGATGAA CAGGCCATGG ACAGCGAGAA CTCCCAAGAG 
AACAATCAGC CTCGTTCAAG TTCCAGTACT ACAGCCAGTA GCAGCCCCAG TACCGTCATC 
CATGGAGTGA ATCATGAGTC TGCAGATTCA GCAGAGATGG AGGAGAAGCT AGTTGCCGGA 
TTTTCAAACC ATCTCCCTTC TTTGCCACTG CAACCAAGCT TTCCCAAGAT GAGCTTTGAT 
CGTCGTGAGA GTGATAGCCC AGCTGTCAGC ATGAGCTCCT CAGAAGGGGT GGTGAGGAAA 
CGAGAGTATG ATAATCCATA CTTCGAACCA CAGTATGGTT TTCCTACAGA GGATGAAGAT 
GATGAGCAGG AAGAGAGCTA CACCCCAAGA TTTAACCAGA ATCTCAATGG AAGCAGGTCT 
CAGAAGTTAC TTCGTCCAAA CAGTTTAAAA CTGGCCAATG ATTCTGATGC AGATTCAGAC 
TCCAGGGCCA GCTCTCCAAA CTCTACTGTC TCCAACAACA GCAGTGAAGG TTTTGGGGGC 
ATCATGTCTT TTGCAAGCAG CCTGTACAGA AATCACAGCA CAAGTTTCAG TCTGTCCAAT 
TTAGCCCTGC CAACCAAAGT TGGGAGAGAC AAGAATACCC CCTTTCCCAG CCTGAAAGAT 
TACTTTAATC TGGAAGTGGG AAGGGATGTG AATGAAGTAT TTGGGTTAAA TACTATAATG 
GAGATTATTA CTGAAGCTGG CCCAGTAAGC AATGAAGGAA ACAGGAGAGC CCTGGTGGAT 
CAAAAGTCTT CAGTCATTAA GCATAGCCCA ACAGTGAAGA GGGAATCTCC ATCACCTCAG 
GGACGAAGTA GTAACTCCAG TGAGAACCAG CAATTCCTGA AGGAGGTGGT ACACAATGTT 
CTCGATGGGC AAGGTGTTGG TTGGCTGAAC ATGAAGAGAG TCCGACGTCT GCTGGAGAGT 
GAGCAGCTCC GTGTCTTTGT ACTAAGCAAG CTAAATCGCA CGATCCAGTC GGAAGAAGAT 
GCTCAGCAGG ATATCATACA GGATGTGGAG ATCAGCCGCA AAGTTTATAA AGGCATGCTG 
GACTTGCTAA AGTGTACTGT GCTGAGCCTG GAGCAGTCAT ACGCAAATGC TGGCCTGGGA 
GGCATGGCCA GTGTTTTTGG CCTTCTAGAA ATAGCACATA CTCACTATTA CAACAAAGAA 
CCAGAAAAGA GAAAACGAAG CCCAACAGAT GGATCTGTCA CTCCGGTTGG CAAAGATCCT 
GCATCATCTC CAAGAGTGGA GCCGAAACCT GCCATGCAGC TGCCAGTACC ACAGCTGATG 
CCGAAGGCAC CAAGCCCTGC AGGGAAAGGG CTAAGGGAGT TTGATACAAG GAGTCTAAAG 
GAAGAAAATT TTATTGCTTC CATTGAATTG TGGAACAAGC ACCAGGAAGT GAAAAAGCAA 
AAATCTTTGG AAAAAACGAG GCCAGAAGGT GTGAAACATC TTGATTTGGG AGAAACAGAT 
GAGAAAAAAT CCCAAATCAG TGCAGACAGT GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG 
AGTGATTTTG ACTCTGTTCC CAGTGGAGGA CCGTCAGTTA TGGTCCGAAG TACCAGCCAG 
GATTCTGAAG TTAGCACAGT GGTTAGTAAC AGCTCTGGGG AGACACTAGG AGCAGACAGT 
GACTTGAGTA GCAATGCTGG TGATGGCCCA AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA 
TCCAGGGGCA CCGTGTCAGA CAGTGAAATT GAGACAAACT CTGCTACTAG CTCTATTTTC 
GCAAAGTCTC ATAACTTGAA GCAGAATGTA AAGGAGAACA AAGGCAGTAC TGCAGGGAAA 
GGTCCAGAGG ATGGGAACCA GCGTGTCTAT CTCTATGAAG GACTTTTGGG TAGGGATAAA 
GGATCTGTCT GGGACCAGTT AGAGGATGCC GCAATGGAAA CCTTCTCTAT GAGCAAAGAG 
CGCTCAACAC TGTGGGACCA GATGCAATTC TGGGAAGATG CCTTTTTGGA TGCTGTGATG 
TTAGAGAGAG AAGGAATGGG GATGGATCAG GGACCCCAGG AGATGATTGA CAGGTATCTT 
TCCCTGGGAG AACATGATCG AAAACGTTTG GAGGATGATG AGGACCGCTT ATTGGCTACG 
CTGCTACATA ATATGATTGC TTATATGCTT ATGATAAAGG TGAATAAGAA TGATATTAGG 
AAAAAGGTGC GTCGTCTGAT GGGAAAATCG CATATTGGAT TGTTGCACAG CCAGCAGTTA 
AATGACATCC TAGACAAGCT TGCTAACCTG AATGGACGGG AACTTCCTGT GAGACCCAGC 
GGCAGCCGCC ACATCAAGAA GCAGACTTTT GTAGTCCATG CTGGGACAGA CACTACAGGA 
GATATATTTT TCATGGAGGT TTGTGATGAC TGCATTGTGC TGAGAAGCAA CATTGGAACT 
GTGTATGAAC GTTGGTGGTA TGAGAAACTC ATCAACATGA CTTACTGTCC CAAAACAAAA 
GTTCTGTGTC TCTGGCGGAG GAATGGTCAG GAAACACAAC TGAACAAGTT CTACACAAAG 
AAGTGTCGGG AATTATACTA CTGCGTAAAA GACAGCATGG AGCGAGCAGC AGCAAGACAG 
CAAAGCATTA AGCCAGGCCC TGAGTTGGGT GGTGAGTTTC CTGTGCAGGA TATGAAAACT 
GGCGAAGGAG GTCTTCTGCA GGTGACACTG GAAGGAATTA ACCTGAAATT TATGCACAGT 
CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT CACATTAAAA AGTGCAATAC TGTGCGAGGA 
GTCTTTGTCC TGGAGGAATT TGTTCCTGAA ACTAAAGAAG TGGTGAGCCA CAAGTACAAG 
ACGCCAATGG CTCACGAGAT CTGCTACTCC GTGCTGTGTC TCTTCTCTTA TGTGGCTGCC 
ATTCGTGGGA AAGAGGCAGA AAACAAAAGC AAACCACCTC GGCCAGTTTC AAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287158.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142644.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X5, mRNA.

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CloneID	OGa36676
Clone ID Related Accession (Same CDS sequence)	XM_015287158.1 , XM_015287158.2
Accession Version	XM_015287158.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGTTT TCATAGAGCT GAATCACATT AAAAAGTGCA ATACTGTGCG AGGAGTCTTT 
GTCCTGGAGG AATTTGTTCC TGAAACTAAA GAAGTGGTGA GCCACAAGTA CAAGACGCCA 
ATGGCTCACG AGATCTGCTA CTCCGTGCTG TGTCTCTTCT CTTATGTGGC TGCCATTCGT 
GGGAAAGAGG CAGAAAACAA AAGCAAACCA CCTCGGCCAG TTTCAAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142644.1
CDS	233..5323
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKDYFNLEVGRDVNEVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287158.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287158.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGTTT TCATAGAGCT GAATCACATT AAAAAGTGCA ATACTGTGCG AGGAGTCTTT 
GTCCTGGAGG AATTTGTTCC TGAAACTAAA GAAGTGGTGA GCCACAAGTA CAAGACGCCA 
ATGGCTCACG AGATCTGCTA CTCCGTGCTG TGTCTCTTCT CTTATGTGGC TGCCATTCGT 
GGGAAAGAGG CAGAAAACAA AAGCAAACCA CCTCGGCCAG TTTCAAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287158.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142644.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X5, mRNA.

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CloneID	OGa36676
Clone ID Related Accession (Same CDS sequence)	XM_015287158.1 , XM_015287158.2
Accession Version	XM_015287158.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287158.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGTTT TCATAGAGCT GAATCACATT AAAAAGTGCA ATACTGTGCG AGGAGTCTTT 
GTCCTGGAGG AATTTGTTCC TGAAACTAAA GAAGTGGTGA GCCACAAGTA CAAGACGCCA 
ATGGCTCACG AGATCTGCTA CTCCGTGCTG TGTCTCTTCT CTTATGTGGC TGCCATTCGT 
GGGAAAGAGG CAGAAAACAA AAGCAAACCA CCTCGGCCAG TTTCAAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142644.1
CDS	277..5367
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKDYFNLEVGRDVNEVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287158.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287158.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGTTT TCATAGAGCT GAATCACATT AAAAAGTGCA ATACTGTGCG AGGAGTCTTT 
GTCCTGGAGG AATTTGTTCC TGAAACTAAA GAAGTGGTGA GCCACAAGTA CAAGACGCCA 
ATGGCTCACG AGATCTGCTA CTCCGTGCTG TGTCTCTTCT CTTATGTGGC TGCCATTCGT 
GGGAAAGAGG CAGAAAACAA AAGCAAACCA CCTCGGCCAG TTTCAAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287160.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142646.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X7, mRNA.

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CloneID	OGa36677
Clone ID Related Accession (Same CDS sequence)	XM_015287160.1 , XM_015287160.2
Accession Version	XM_015287160.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGGG CCAGAAGGTG TGAAACATCT TGATTTGGGA 
GAAACAGATG AGAAAAAATC CCAAATCAGT GCAGACAGTG GCCTCAGTTT GGCCTCAGGT 
TCTCAGAAGA GTGATTTTGA CTCTGTTCCC AGTGGAGGAC CGTCAGTTAT GGTCCGAAGT 
ACCAGCCAGG ATTCTGAAGT TAGCACAGTG GTTAGTAACA GCTCTGGGGA GACACTAGGA 
GCAGACAGTG ACTTGAGTAG CAATGCTGGT GATGGCCCAA GTGTGGAAAA TGGAGGCAAT 
CTGGCAGGAT CCAGGGGCAC CGTGTCAGAC AGTGAAATTG AGACAAACTC TGCTACTAGC 
TCTATTTTCG CAAAGTCTCA TAACTTGAAG CAGAATGTAA AGGAGAACAA AGGCAGTACT 
GCAGGGAAAG GTCCAGAGGA TGGGAACCAG CGTGTCTATC TCTATGAAGG ACTTTTGGGT 
AGGGATAAAG GATCTGTCTG GGACCAGTTA GAGGATGCCG CAATGGAAAC CTTCTCTATG 
AGCAAAGAGC GCTCAACACT GTGGGACCAG ATGCAATTCT GGGAAGATGC CTTTTTGGAT 
GCTGTGATGT TAGAGAGAGA AGGAATGGGG ATGGATCAGG GACCCCAGGA GATGATTGAC 
AGGTATCTTT CCCTGGGAGA ACATGATCGA AAACGTTTGG AGGATGATGA GGACCGCTTA 
TTGGCTACGC TGCTACATAA TATGATTGCT TATATGCTTA TGATAAAGGT GAATAAGAAT 
GATATTAGGA AAAAGGTGCG TCGTCTGATG GGAAAATCGC ATATTGGATT GTTGCACAGC 
CAGCAGTTAA ATGACATCCT AGACAAGCTT GCTAACCTGA ATGGACGGGA ACTTCCTGTG 
AGACCCAGCG GCAGCCGCCA CATCAAGAAG CAGACTTTTG TAGTCCATGC TGGGACAGAC 
ACTACAGGAG ATATATTTTT CATGGAGGTT TGTGATGACT GCATTGTGCT GAGAAGCAAC 
ATTGGAACTG TGTATGAACG TTGGTGGTAT GAGAAACTCA TCAACATGAC TTACTGTCCC 
AAAACAAAAG TTCTGTGTCT CTGGCGGAGG AATGGTCAGG AAACACAACT GAACAAGTTC 
TACACAAAGA AGTGTCGGGA ATTATACTAC TGCGTAAAAG ACAGCATGGA GCGAGCAGCA 
GCAAGACAGC AAAGCATTAA GCCAGGCCCT GAGTTGGGTG GTGAGTTTCC TGTGCAGGAT 
ATGAAAACTG GCGAAGGAGG TCTTCTGCAG GTGACACTGG AAGGAATTAA CCTGAAATTT 
ATGCACAGTC AGGAGCGGAA GGTTTTCATA GAGCTGAATC ACATTAAAAA GTGCAATACT 
GTGCGAGGAG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC 
AAGTACAAGA CGCCAATGGC TCACGAGATC TGCTACTCCG TGCTGTGTCT CTTCTCTTAT 
GTGGCTGCCA TTCGTGGGAA AGAGGCAGAA AACAAAAGCA AACCACCTCG GCCAGTTTCA 
AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142646.1
CDS	233..5278
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKDYFNLEVGRDVNEVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIGPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287160.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287160.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGGG CCAGAAGGTG TGAAACATCT TGATTTGGGA 
GAAACAGATG AGAAAAAATC CCAAATCAGT GCAGACAGTG GCCTCAGTTT GGCCTCAGGT 
TCTCAGAAGA GTGATTTTGA CTCTGTTCCC AGTGGAGGAC CGTCAGTTAT GGTCCGAAGT 
ACCAGCCAGG ATTCTGAAGT TAGCACAGTG GTTAGTAACA GCTCTGGGGA GACACTAGGA 
GCAGACAGTG ACTTGAGTAG CAATGCTGGT GATGGCCCAA GTGTGGAAAA TGGAGGCAAT 
CTGGCAGGAT CCAGGGGCAC CGTGTCAGAC AGTGAAATTG AGACAAACTC TGCTACTAGC 
TCTATTTTCG CAAAGTCTCA TAACTTGAAG CAGAATGTAA AGGAGAACAA AGGCAGTACT 
GCAGGGAAAG GTCCAGAGGA TGGGAACCAG CGTGTCTATC TCTATGAAGG ACTTTTGGGT 
AGGGATAAAG GATCTGTCTG GGACCAGTTA GAGGATGCCG CAATGGAAAC CTTCTCTATG 
AGCAAAGAGC GCTCAACACT GTGGGACCAG ATGCAATTCT GGGAAGATGC CTTTTTGGAT 
GCTGTGATGT TAGAGAGAGA AGGAATGGGG ATGGATCAGG GACCCCAGGA GATGATTGAC 
AGGTATCTTT CCCTGGGAGA ACATGATCGA AAACGTTTGG AGGATGATGA GGACCGCTTA 
TTGGCTACGC TGCTACATAA TATGATTGCT TATATGCTTA TGATAAAGGT GAATAAGAAT 
GATATTAGGA AAAAGGTGCG TCGTCTGATG GGAAAATCGC ATATTGGATT GTTGCACAGC 
CAGCAGTTAA ATGACATCCT AGACAAGCTT GCTAACCTGA ATGGACGGGA ACTTCCTGTG 
AGACCCAGCG GCAGCCGCCA CATCAAGAAG CAGACTTTTG TAGTCCATGC TGGGACAGAC 
ACTACAGGAG ATATATTTTT CATGGAGGTT TGTGATGACT GCATTGTGCT GAGAAGCAAC 
ATTGGAACTG TGTATGAACG TTGGTGGTAT GAGAAACTCA TCAACATGAC TTACTGTCCC 
AAAACAAAAG TTCTGTGTCT CTGGCGGAGG AATGGTCAGG AAACACAACT GAACAAGTTC 
TACACAAAGA AGTGTCGGGA ATTATACTAC TGCGTAAAAG ACAGCATGGA GCGAGCAGCA 
GCAAGACAGC AAAGCATTAA GCCAGGCCCT GAGTTGGGTG GTGAGTTTCC TGTGCAGGAT 
ATGAAAACTG GCGAAGGAGG TCTTCTGCAG GTGACACTGG AAGGAATTAA CCTGAAATTT 
ATGCACAGTC AGGAGCGGAA GGTTTTCATA GAGCTGAATC ACATTAAAAA GTGCAATACT 
GTGCGAGGAG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC 
AAGTACAAGA CGCCAATGGC TCACGAGATC TGCTACTCCG TGCTGTGTCT CTTCTCTTAT 
GTGGCTGCCA TTCGTGGGAA AGAGGCAGAA AACAAAAGCA AACCACCTCG GCCAGTTTCA 
AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287160.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142646.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X7, mRNA.

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CloneID	OGa36677
Clone ID Related Accession (Same CDS sequence)	XM_015287160.1 , XM_015287160.2
Accession Version	XM_015287160.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287160.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGGG CCAGAAGGTG TGAAACATCT TGATTTGGGA 
GAAACAGATG AGAAAAAATC CCAAATCAGT GCAGACAGTG GCCTCAGTTT GGCCTCAGGT 
TCTCAGAAGA GTGATTTTGA CTCTGTTCCC AGTGGAGGAC CGTCAGTTAT GGTCCGAAGT 
ACCAGCCAGG ATTCTGAAGT TAGCACAGTG GTTAGTAACA GCTCTGGGGA GACACTAGGA 
GCAGACAGTG ACTTGAGTAG CAATGCTGGT GATGGCCCAA GTGTGGAAAA TGGAGGCAAT 
CTGGCAGGAT CCAGGGGCAC CGTGTCAGAC AGTGAAATTG AGACAAACTC TGCTACTAGC 
TCTATTTTCG CAAAGTCTCA TAACTTGAAG CAGAATGTAA AGGAGAACAA AGGCAGTACT 
GCAGGGAAAG GTCCAGAGGA TGGGAACCAG CGTGTCTATC TCTATGAAGG ACTTTTGGGT 
AGGGATAAAG GATCTGTCTG GGACCAGTTA GAGGATGCCG CAATGGAAAC CTTCTCTATG 
AGCAAAGAGC GCTCAACACT GTGGGACCAG ATGCAATTCT GGGAAGATGC CTTTTTGGAT 
GCTGTGATGT TAGAGAGAGA AGGAATGGGG ATGGATCAGG GACCCCAGGA GATGATTGAC 
AGGTATCTTT CCCTGGGAGA ACATGATCGA AAACGTTTGG AGGATGATGA GGACCGCTTA 
TTGGCTACGC TGCTACATAA TATGATTGCT TATATGCTTA TGATAAAGGT GAATAAGAAT 
GATATTAGGA AAAAGGTGCG TCGTCTGATG GGAAAATCGC ATATTGGATT GTTGCACAGC 
CAGCAGTTAA ATGACATCCT AGACAAGCTT GCTAACCTGA ATGGACGGGA ACTTCCTGTG 
AGACCCAGCG GCAGCCGCCA CATCAAGAAG CAGACTTTTG TAGTCCATGC TGGGACAGAC 
ACTACAGGAG ATATATTTTT CATGGAGGTT TGTGATGACT GCATTGTGCT GAGAAGCAAC 
ATTGGAACTG TGTATGAACG TTGGTGGTAT GAGAAACTCA TCAACATGAC TTACTGTCCC 
AAAACAAAAG TTCTGTGTCT CTGGCGGAGG AATGGTCAGG AAACACAACT GAACAAGTTC 
TACACAAAGA AGTGTCGGGA ATTATACTAC TGCGTAAAAG ACAGCATGGA GCGAGCAGCA 
GCAAGACAGC AAAGCATTAA GCCAGGCCCT GAGTTGGGTG GTGAGTTTCC TGTGCAGGAT 
ATGAAAACTG GCGAAGGAGG TCTTCTGCAG GTGACACTGG AAGGAATTAA CCTGAAATTT 
ATGCACAGTC AGGAGCGGAA GGTTTTCATA GAGCTGAATC ACATTAAAAA GTGCAATACT 
GTGCGAGGAG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC 
AAGTACAAGA CGCCAATGGC TCACGAGATC TGCTACTCCG TGCTGTGTCT CTTCTCTTAT 
GTGGCTGCCA TTCGTGGGAA AGAGGCAGAA AACAAAAGCA AACCACCTCG GCCAGTTTCA 
AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142646.1
CDS	277..5322
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKDYFNLEVGRDVNEVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIGPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287160.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287160.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGGG CCAGAAGGTG TGAAACATCT TGATTTGGGA 
GAAACAGATG AGAAAAAATC CCAAATCAGT GCAGACAGTG GCCTCAGTTT GGCCTCAGGT 
TCTCAGAAGA GTGATTTTGA CTCTGTTCCC AGTGGAGGAC CGTCAGTTAT GGTCCGAAGT 
ACCAGCCAGG ATTCTGAAGT TAGCACAGTG GTTAGTAACA GCTCTGGGGA GACACTAGGA 
GCAGACAGTG ACTTGAGTAG CAATGCTGGT GATGGCCCAA GTGTGGAAAA TGGAGGCAAT 
CTGGCAGGAT CCAGGGGCAC CGTGTCAGAC AGTGAAATTG AGACAAACTC TGCTACTAGC 
TCTATTTTCG CAAAGTCTCA TAACTTGAAG CAGAATGTAA AGGAGAACAA AGGCAGTACT 
GCAGGGAAAG GTCCAGAGGA TGGGAACCAG CGTGTCTATC TCTATGAAGG ACTTTTGGGT 
AGGGATAAAG GATCTGTCTG GGACCAGTTA GAGGATGCCG CAATGGAAAC CTTCTCTATG 
AGCAAAGAGC GCTCAACACT GTGGGACCAG ATGCAATTCT GGGAAGATGC CTTTTTGGAT 
GCTGTGATGT TAGAGAGAGA AGGAATGGGG ATGGATCAGG GACCCCAGGA GATGATTGAC 
AGGTATCTTT CCCTGGGAGA ACATGATCGA AAACGTTTGG AGGATGATGA GGACCGCTTA 
TTGGCTACGC TGCTACATAA TATGATTGCT TATATGCTTA TGATAAAGGT GAATAAGAAT 
GATATTAGGA AAAAGGTGCG TCGTCTGATG GGAAAATCGC ATATTGGATT GTTGCACAGC 
CAGCAGTTAA ATGACATCCT AGACAAGCTT GCTAACCTGA ATGGACGGGA ACTTCCTGTG 
AGACCCAGCG GCAGCCGCCA CATCAAGAAG CAGACTTTTG TAGTCCATGC TGGGACAGAC 
ACTACAGGAG ATATATTTTT CATGGAGGTT TGTGATGACT GCATTGTGCT GAGAAGCAAC 
ATTGGAACTG TGTATGAACG TTGGTGGTAT GAGAAACTCA TCAACATGAC TTACTGTCCC 
AAAACAAAAG TTCTGTGTCT CTGGCGGAGG AATGGTCAGG AAACACAACT GAACAAGTTC 
TACACAAAGA AGTGTCGGGA ATTATACTAC TGCGTAAAAG ACAGCATGGA GCGAGCAGCA 
GCAAGACAGC AAAGCATTAA GCCAGGCCCT GAGTTGGGTG GTGAGTTTCC TGTGCAGGAT 
ATGAAAACTG GCGAAGGAGG TCTTCTGCAG GTGACACTGG AAGGAATTAA CCTGAAATTT 
ATGCACAGTC AGGAGCGGAA GGTTTTCATA GAGCTGAATC ACATTAAAAA GTGCAATACT 
GTGCGAGGAG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA CTAAAGAAGT GGTGAGCCAC 
AAGTACAAGA CGCCAATGGC TCACGAGATC TGCTACTCCG TGCTGTGTCT CTTCTCTTAT 
GTGGCTGCCA TTCGTGGGAA AGAGGCAGAA AACAAAAGCA AACCACCTCG GCCAGTTTCA 
AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287161.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142647.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OGa36678
Clone ID Related Accession (Same CDS sequence)	XM_015287161.1 , XM_015287161.2
Accession Version	XM_015287161.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG CAGTGGCAGT GACAGTGTCG ACTATGATGA CTCAAGTTCC 
TCCTACTCTT CCCTTGGAGA CTTTGTCAGT GAGATGATGA AATGTGACAT TAATGGCGAT 
ACTCCAAATG TTGACCCCCT GACTCACGCA GCCCTTGGTG ATGCCAGTGA AGTGGAGTTC 
GATGATTTTC AAGAATATTC AGGAGAGCTG GATGAACAGG CCATGGACAG CGAGAACTCC 
CAAGAGAACA ATCAGCCTCG TTCAAGTTCC AGTACTACAG CCAGTAGCAG CCCCAGTACC 
GTCATCCATG GAGTGAATCA TGAGTCTGCA GATTCAGCAG AGATGGAGGA GAAGCTAGTT 
GCCGGATTTT CAAACCATCT CCCTTCTTTG CCACTGCAAC CAAGCTTTCC CAAGATGAGC 
TTTGATCGTC GTGAGAGTGA TAGCCCAGCT GTCAGCATGA GCTCCTCAGA AGGGGTGGTG 
AGGAAACGAG AGTATGATAA TCCATACTTC GAACCACAGT ATGGTTTTCC TACAGAGGAT 
GAAGATGATG AGCAGGAAGA GAGCTACACC CCAAGATTTA ACCAGAATCT CAATGGAAGC 
AGGTCTCAGA AGTTACTTCG TCCAAACAGT TTAAAACTGG CCAATGATTC TGATGCAGAT 
TCAGACTCCA GGGCCAGCTC TCCAAACTCT ACTGTCTCCA ACAACAGCAG TGAAGGTTTT 
GGGGGCATCA TGTCTTTTGC AAGCAGCCTG TACAGAAATC ACAGCACAAG TTTCAGTCTG 
TCCAATTTAG CCCTGCCAAC CAAAGTTGGG AGAGACAAGA ATACCCCCTT TCCCAGCCTG 
AAAGATTACT TTAATCTGGA AGTGGGAAGG GATGTGAATG AAGTATTTGG GTTAAATACT 
ATAATGGAGA TTATTACTGA AGCTGGCCCA GTAAGCAATG AAGGAAACAG GAGAGCCCTG 
GTGGATCAAA AGTCTTCAGT CATTAAGCAT AGCCCAACAG TGAAGAGGGA ATCTCCATCA 
CCTCAGGGAC GAAGTAGTAA CTCCAGTGAG AACCAGCAAT TCCTGAAGGA GGTGGTACAC 
AATGTTCTCG ATGGGCAAGG TGTTGGTTGG CTGAACATGA AGAGAGTCCG ACGTCTGCTG 
GAGAGTGAGC AGCTCCGTGT CTTTGTACTA AGCAAGCTAA ATCGCACGAT CCAGTCGGAA 
GAAGATGCTC AGCAGGATAT CATACAGGAT GTGGAGATCA GCCGCAAAGT TTATAAAGGC 
ATGCTGGACT TGCTAAAGTG TACTGTGCTG AGCCTGGAGC AGTCATACGC AAATGCTGGC 
CTGGGAGGCA TGGCCAGTGT TTTTGGCCTT CTAGAAATAG CACATACTCA CTATTACAAC 
AAAGAACCAG AAAAGAGAAA ACGAAGCCCA ACAGATGGAT CTGTCACTCC GGTTGGCAAA 
GATCCTGCAT CATCTCCAAG AGTGGAGCCG AAACCTGCCA TGCAGCTGCC AGTACCACAG 
CTGATGCCGA AGGCACCAAG CCCTGCAGGG AAAGGGCTAA GGGAGTTTGA TACAAGGAGT 
CTAAAGGAAG AAAATTTTAT TGCTTCCATT GAATTGTGGA ACAAGCACCA GGAAGTGAAA 
AAGCAAAAAT CTTTGGAAAA AACGAGGCCA GAAGGTGTGA AACATCTTGA TTTGGGAGAA 
ACAGATGAGA AAAAATCCCA AATCAGTGCA GACAGTGGCC TCAGTTTGGC CTCAGGTTCT 
CAGAAGAGTG ATTTTGACTC TGTTCCCAGT GGAGGACCGT CAGTTATGGT CCGAAGTACC 
AGCCAGGATT CTGAAGTTAG CACAGTGGTT AGTAACAGCT CTGGGGAGAC ACTAGGAGCA 
GACAGTGACT TGAGTAGCAA TGCTGGTGAT GGCCCAAGTG TGGAAAATGG AGGCAATCTG 
GCAGGATCCA GGGGCACCGT GTCAGACAGT GAAATTGAGA CAAACTCTGC TACTAGCTCT 
ATTTTCGCAA AGTCTCATAA CTTGAAGCAG AATGTAAAGG AGAACAAAGG CAGTACTGCA 
GGGAAAGGTC CAGAGGATGG GAACCAGCGT GTCTATCTCT ATGAAGGACT TTTGGGTAGG 
GATAAAGGAT CTGTCTGGGA CCAGTTAGAG GATGCCGCAA TGGAAACCTT CTCTATGAGC 
AAAGAGCGCT CAACACTGTG GGACCAGATG CAATTCTGGG AAGATGCCTT TTTGGATGCT 
GTGATGTTAG AGAGAGAAGG AATGGGGATG GATCAGGGAC CCCAGGAGAT GATTGACAGG 
TATCTTTCCC TGGGAGAACA TGATCGAAAA CGTTTGGAGG ATGATGAGGA CCGCTTATTG 
GCTACGCTGC TACATAATAT GATTGCTTAT ATGCTTATGA TAAAGGTGAA TAAGAATGAT 
ATTAGGAAAA AGGTGCGTCG TCTGATGGGA AAATCGCATA TTGGATTGTT GCACAGCCAG 
CAGTTAAATG ACATCCTAGA CAAGCTTGCT AACCTGAATG GACGGGAACT TCCTGTGAGA 
CCCAGCGGCA GCCGCCACAT CAAGAAGCAG ACTTTTGTAG TCCATGCTGG GACAGACACT 
ACAGGAGATA TATTTTTCAT GGAGGTTTGT GATGACTGCA TTGTGCTGAG AAGCAACATT 
GGAACTGTGT ATGAACGTTG GTGGTATGAG AAACTCATCA ACATGACTTA CTGTCCCAAA 
ACAAAAGTTC TGTGTCTCTG GCGGAGGAAT GGTCAGGAAA CACAACTGAA CAAGTTCTAC 
ACAAAGAAGT GTCGGGAATT ATACTACTGC GTAAAAGACA GCATGGAGCG AGCAGCAGCA 
AGACAGCAAA GCATTAAGCC AGGCCCTGAG TTGGGTGGTG AGTTTCCTGT GCAGGATATG 
AAAACTGGCG AAGGAGGTCT TCTGCAGGTG ACACTGGAAG GAATTAACCT GAAATTTATG 
CACAGTCAGG AGCGGAAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACTGTG 
CGAGGAGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAG 
TACAAGACGC CAATGGCTCA CGAGATCTGC TACTCCGTGC TGTGTCTCTT CTCTTATGTG 
GCTGCCATTC GTGGGAAAGA GGCAGAAAAC AAAAGCAAAC CACCTCGGCC AGTTTCAAGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142647.1
CDS	233..5275
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKDYFNLEVGRDVNEVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287161.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287161.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG CAGTGGCAGT GACAGTGTCG ACTATGATGA CTCAAGTTCC 
TCCTACTCTT CCCTTGGAGA CTTTGTCAGT GAGATGATGA AATGTGACAT TAATGGCGAT 
ACTCCAAATG TTGACCCCCT GACTCACGCA GCCCTTGGTG ATGCCAGTGA AGTGGAGTTC 
GATGATTTTC AAGAATATTC AGGAGAGCTG GATGAACAGG CCATGGACAG CGAGAACTCC 
CAAGAGAACA ATCAGCCTCG TTCAAGTTCC AGTACTACAG CCAGTAGCAG CCCCAGTACC 
GTCATCCATG GAGTGAATCA TGAGTCTGCA GATTCAGCAG AGATGGAGGA GAAGCTAGTT 
GCCGGATTTT CAAACCATCT CCCTTCTTTG CCACTGCAAC CAAGCTTTCC CAAGATGAGC 
TTTGATCGTC GTGAGAGTGA TAGCCCAGCT GTCAGCATGA GCTCCTCAGA AGGGGTGGTG 
AGGAAACGAG AGTATGATAA TCCATACTTC GAACCACAGT ATGGTTTTCC TACAGAGGAT 
GAAGATGATG AGCAGGAAGA GAGCTACACC CCAAGATTTA ACCAGAATCT CAATGGAAGC 
AGGTCTCAGA AGTTACTTCG TCCAAACAGT TTAAAACTGG CCAATGATTC TGATGCAGAT 
TCAGACTCCA GGGCCAGCTC TCCAAACTCT ACTGTCTCCA ACAACAGCAG TGAAGGTTTT 
GGGGGCATCA TGTCTTTTGC AAGCAGCCTG TACAGAAATC ACAGCACAAG TTTCAGTCTG 
TCCAATTTAG CCCTGCCAAC CAAAGTTGGG AGAGACAAGA ATACCCCCTT TCCCAGCCTG 
AAAGATTACT TTAATCTGGA AGTGGGAAGG GATGTGAATG AAGTATTTGG GTTAAATACT 
ATAATGGAGA TTATTACTGA AGCTGGCCCA GTAAGCAATG AAGGAAACAG GAGAGCCCTG 
GTGGATCAAA AGTCTTCAGT CATTAAGCAT AGCCCAACAG TGAAGAGGGA ATCTCCATCA 
CCTCAGGGAC GAAGTAGTAA CTCCAGTGAG AACCAGCAAT TCCTGAAGGA GGTGGTACAC 
AATGTTCTCG ATGGGCAAGG TGTTGGTTGG CTGAACATGA AGAGAGTCCG ACGTCTGCTG 
GAGAGTGAGC AGCTCCGTGT CTTTGTACTA AGCAAGCTAA ATCGCACGAT CCAGTCGGAA 
GAAGATGCTC AGCAGGATAT CATACAGGAT GTGGAGATCA GCCGCAAAGT TTATAAAGGC 
ATGCTGGACT TGCTAAAGTG TACTGTGCTG AGCCTGGAGC AGTCATACGC AAATGCTGGC 
CTGGGAGGCA TGGCCAGTGT TTTTGGCCTT CTAGAAATAG CACATACTCA CTATTACAAC 
AAAGAACCAG AAAAGAGAAA ACGAAGCCCA ACAGATGGAT CTGTCACTCC GGTTGGCAAA 
GATCCTGCAT CATCTCCAAG AGTGGAGCCG AAACCTGCCA TGCAGCTGCC AGTACCACAG 
CTGATGCCGA AGGCACCAAG CCCTGCAGGG AAAGGGCTAA GGGAGTTTGA TACAAGGAGT 
CTAAAGGAAG AAAATTTTAT TGCTTCCATT GAATTGTGGA ACAAGCACCA GGAAGTGAAA 
AAGCAAAAAT CTTTGGAAAA AACGAGGCCA GAAGGTGTGA AACATCTTGA TTTGGGAGAA 
ACAGATGAGA AAAAATCCCA AATCAGTGCA GACAGTGGCC TCAGTTTGGC CTCAGGTTCT 
CAGAAGAGTG ATTTTGACTC TGTTCCCAGT GGAGGACCGT CAGTTATGGT CCGAAGTACC 
AGCCAGGATT CTGAAGTTAG CACAGTGGTT AGTAACAGCT CTGGGGAGAC ACTAGGAGCA 
GACAGTGACT TGAGTAGCAA TGCTGGTGAT GGCCCAAGTG TGGAAAATGG AGGCAATCTG 
GCAGGATCCA GGGGCACCGT GTCAGACAGT GAAATTGAGA CAAACTCTGC TACTAGCTCT 
ATTTTCGCAA AGTCTCATAA CTTGAAGCAG AATGTAAAGG AGAACAAAGG CAGTACTGCA 
GGGAAAGGTC CAGAGGATGG GAACCAGCGT GTCTATCTCT ATGAAGGACT TTTGGGTAGG 
GATAAAGGAT CTGTCTGGGA CCAGTTAGAG GATGCCGCAA TGGAAACCTT CTCTATGAGC 
AAAGAGCGCT CAACACTGTG GGACCAGATG CAATTCTGGG AAGATGCCTT TTTGGATGCT 
GTGATGTTAG AGAGAGAAGG AATGGGGATG GATCAGGGAC CCCAGGAGAT GATTGACAGG 
TATCTTTCCC TGGGAGAACA TGATCGAAAA CGTTTGGAGG ATGATGAGGA CCGCTTATTG 
GCTACGCTGC TACATAATAT GATTGCTTAT ATGCTTATGA TAAAGGTGAA TAAGAATGAT 
ATTAGGAAAA AGGTGCGTCG TCTGATGGGA AAATCGCATA TTGGATTGTT GCACAGCCAG 
CAGTTAAATG ACATCCTAGA CAAGCTTGCT AACCTGAATG GACGGGAACT TCCTGTGAGA 
CCCAGCGGCA GCCGCCACAT CAAGAAGCAG ACTTTTGTAG TCCATGCTGG GACAGACACT 
ACAGGAGATA TATTTTTCAT GGAGGTTTGT GATGACTGCA TTGTGCTGAG AAGCAACATT 
GGAACTGTGT ATGAACGTTG GTGGTATGAG AAACTCATCA ACATGACTTA CTGTCCCAAA 
ACAAAAGTTC TGTGTCTCTG GCGGAGGAAT GGTCAGGAAA CACAACTGAA CAAGTTCTAC 
ACAAAGAAGT GTCGGGAATT ATACTACTGC GTAAAAGACA GCATGGAGCG AGCAGCAGCA 
AGACAGCAAA GCATTAAGCC AGGCCCTGAG TTGGGTGGTG AGTTTCCTGT GCAGGATATG 
AAAACTGGCG AAGGAGGTCT TCTGCAGGTG ACACTGGAAG GAATTAACCT GAAATTTATG 
CACAGTCAGG AGCGGAAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACTGTG 
CGAGGAGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAG 
TACAAGACGC CAATGGCTCA CGAGATCTGC TACTCCGTGC TGTGTCTCTT CTCTTATGTG 
GCTGCCATTC GTGGGAAAGA GGCAGAAAAC AAAAGCAAAC CACCTCGGCC AGTTTCAAGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287161.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142647.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X8, mRNA.

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CloneID	OGa36678
Clone ID Related Accession (Same CDS sequence)	XM_015287161.1 , XM_015287161.2
Accession Version	XM_015287161.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287161.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG CAGTGGCAGT GACAGTGTCG ACTATGATGA CTCAAGTTCC 
TCCTACTCTT CCCTTGGAGA CTTTGTCAGT GAGATGATGA AATGTGACAT TAATGGCGAT 
ACTCCAAATG TTGACCCCCT GACTCACGCA GCCCTTGGTG ATGCCAGTGA AGTGGAGTTC 
GATGATTTTC AAGAATATTC AGGAGAGCTG GATGAACAGG CCATGGACAG CGAGAACTCC 
CAAGAGAACA ATCAGCCTCG TTCAAGTTCC AGTACTACAG CCAGTAGCAG CCCCAGTACC 
GTCATCCATG GAGTGAATCA TGAGTCTGCA GATTCAGCAG AGATGGAGGA GAAGCTAGTT 
GCCGGATTTT CAAACCATCT CCCTTCTTTG CCACTGCAAC CAAGCTTTCC CAAGATGAGC 
TTTGATCGTC GTGAGAGTGA TAGCCCAGCT GTCAGCATGA GCTCCTCAGA AGGGGTGGTG 
AGGAAACGAG AGTATGATAA TCCATACTTC GAACCACAGT ATGGTTTTCC TACAGAGGAT 
GAAGATGATG AGCAGGAAGA GAGCTACACC CCAAGATTTA ACCAGAATCT CAATGGAAGC 
AGGTCTCAGA AGTTACTTCG TCCAAACAGT TTAAAACTGG CCAATGATTC TGATGCAGAT 
TCAGACTCCA GGGCCAGCTC TCCAAACTCT ACTGTCTCCA ACAACAGCAG TGAAGGTTTT 
GGGGGCATCA TGTCTTTTGC AAGCAGCCTG TACAGAAATC ACAGCACAAG TTTCAGTCTG 
TCCAATTTAG CCCTGCCAAC CAAAGTTGGG AGAGACAAGA ATACCCCCTT TCCCAGCCTG 
AAAGATTACT TTAATCTGGA AGTGGGAAGG GATGTGAATG AAGTATTTGG GTTAAATACT 
ATAATGGAGA TTATTACTGA AGCTGGCCCA GTAAGCAATG AAGGAAACAG GAGAGCCCTG 
GTGGATCAAA AGTCTTCAGT CATTAAGCAT AGCCCAACAG TGAAGAGGGA ATCTCCATCA 
CCTCAGGGAC GAAGTAGTAA CTCCAGTGAG AACCAGCAAT TCCTGAAGGA GGTGGTACAC 
AATGTTCTCG ATGGGCAAGG TGTTGGTTGG CTGAACATGA AGAGAGTCCG ACGTCTGCTG 
GAGAGTGAGC AGCTCCGTGT CTTTGTACTA AGCAAGCTAA ATCGCACGAT CCAGTCGGAA 
GAAGATGCTC AGCAGGATAT CATACAGGAT GTGGAGATCA GCCGCAAAGT TTATAAAGGC 
ATGCTGGACT TGCTAAAGTG TACTGTGCTG AGCCTGGAGC AGTCATACGC AAATGCTGGC 
CTGGGAGGCA TGGCCAGTGT TTTTGGCCTT CTAGAAATAG CACATACTCA CTATTACAAC 
AAAGAACCAG AAAAGAGAAA ACGAAGCCCA ACAGATGGAT CTGTCACTCC GGTTGGCAAA 
GATCCTGCAT CATCTCCAAG AGTGGAGCCG AAACCTGCCA TGCAGCTGCC AGTACCACAG 
CTGATGCCGA AGGCACCAAG CCCTGCAGGG AAAGGGCTAA GGGAGTTTGA TACAAGGAGT 
CTAAAGGAAG AAAATTTTAT TGCTTCCATT GAATTGTGGA ACAAGCACCA GGAAGTGAAA 
AAGCAAAAAT CTTTGGAAAA AACGAGGCCA GAAGGTGTGA AACATCTTGA TTTGGGAGAA 
ACAGATGAGA AAAAATCCCA AATCAGTGCA GACAGTGGCC TCAGTTTGGC CTCAGGTTCT 
CAGAAGAGTG ATTTTGACTC TGTTCCCAGT GGAGGACCGT CAGTTATGGT CCGAAGTACC 
AGCCAGGATT CTGAAGTTAG CACAGTGGTT AGTAACAGCT CTGGGGAGAC ACTAGGAGCA 
GACAGTGACT TGAGTAGCAA TGCTGGTGAT GGCCCAAGTG TGGAAAATGG AGGCAATCTG 
GCAGGATCCA GGGGCACCGT GTCAGACAGT GAAATTGAGA CAAACTCTGC TACTAGCTCT 
ATTTTCGCAA AGTCTCATAA CTTGAAGCAG AATGTAAAGG AGAACAAAGG CAGTACTGCA 
GGGAAAGGTC CAGAGGATGG GAACCAGCGT GTCTATCTCT ATGAAGGACT TTTGGGTAGG 
GATAAAGGAT CTGTCTGGGA CCAGTTAGAG GATGCCGCAA TGGAAACCTT CTCTATGAGC 
AAAGAGCGCT CAACACTGTG GGACCAGATG CAATTCTGGG AAGATGCCTT TTTGGATGCT 
GTGATGTTAG AGAGAGAAGG AATGGGGATG GATCAGGGAC CCCAGGAGAT GATTGACAGG 
TATCTTTCCC TGGGAGAACA TGATCGAAAA CGTTTGGAGG ATGATGAGGA CCGCTTATTG 
GCTACGCTGC TACATAATAT GATTGCTTAT ATGCTTATGA TAAAGGTGAA TAAGAATGAT 
ATTAGGAAAA AGGTGCGTCG TCTGATGGGA AAATCGCATA TTGGATTGTT GCACAGCCAG 
CAGTTAAATG ACATCCTAGA CAAGCTTGCT AACCTGAATG GACGGGAACT TCCTGTGAGA 
CCCAGCGGCA GCCGCCACAT CAAGAAGCAG ACTTTTGTAG TCCATGCTGG GACAGACACT 
ACAGGAGATA TATTTTTCAT GGAGGTTTGT GATGACTGCA TTGTGCTGAG AAGCAACATT 
GGAACTGTGT ATGAACGTTG GTGGTATGAG AAACTCATCA ACATGACTTA CTGTCCCAAA 
ACAAAAGTTC TGTGTCTCTG GCGGAGGAAT GGTCAGGAAA CACAACTGAA CAAGTTCTAC 
ACAAAGAAGT GTCGGGAATT ATACTACTGC GTAAAAGACA GCATGGAGCG AGCAGCAGCA 
AGACAGCAAA GCATTAAGCC AGGCCCTGAG TTGGGTGGTG AGTTTCCTGT GCAGGATATG 
AAAACTGGCG AAGGAGGTCT TCTGCAGGTG ACACTGGAAG GAATTAACCT GAAATTTATG 
CACAGTCAGG AGCGGAAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACTGTG 
CGAGGAGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAG 
TACAAGACGC CAATGGCTCA CGAGATCTGC TACTCCGTGC TGTGTCTCTT CTCTTATGTG 
GCTGCCATTC GTGGGAAAGA GGCAGAAAAC AAAAGCAAAC CACCTCGGCC AGTTTCAAGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142647.1
CDS	277..5319
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKDYFNLEVGRDVNEVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287161.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287161.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG CAGTGGCAGT GACAGTGTCG ACTATGATGA CTCAAGTTCC 
TCCTACTCTT CCCTTGGAGA CTTTGTCAGT GAGATGATGA AATGTGACAT TAATGGCGAT 
ACTCCAAATG TTGACCCCCT GACTCACGCA GCCCTTGGTG ATGCCAGTGA AGTGGAGTTC 
GATGATTTTC AAGAATATTC AGGAGAGCTG GATGAACAGG CCATGGACAG CGAGAACTCC 
CAAGAGAACA ATCAGCCTCG TTCAAGTTCC AGTACTACAG CCAGTAGCAG CCCCAGTACC 
GTCATCCATG GAGTGAATCA TGAGTCTGCA GATTCAGCAG AGATGGAGGA GAAGCTAGTT 
GCCGGATTTT CAAACCATCT CCCTTCTTTG CCACTGCAAC CAAGCTTTCC CAAGATGAGC 
TTTGATCGTC GTGAGAGTGA TAGCCCAGCT GTCAGCATGA GCTCCTCAGA AGGGGTGGTG 
AGGAAACGAG AGTATGATAA TCCATACTTC GAACCACAGT ATGGTTTTCC TACAGAGGAT 
GAAGATGATG AGCAGGAAGA GAGCTACACC CCAAGATTTA ACCAGAATCT CAATGGAAGC 
AGGTCTCAGA AGTTACTTCG TCCAAACAGT TTAAAACTGG CCAATGATTC TGATGCAGAT 
TCAGACTCCA GGGCCAGCTC TCCAAACTCT ACTGTCTCCA ACAACAGCAG TGAAGGTTTT 
GGGGGCATCA TGTCTTTTGC AAGCAGCCTG TACAGAAATC ACAGCACAAG TTTCAGTCTG 
TCCAATTTAG CCCTGCCAAC CAAAGTTGGG AGAGACAAGA ATACCCCCTT TCCCAGCCTG 
AAAGATTACT TTAATCTGGA AGTGGGAAGG GATGTGAATG AAGTATTTGG GTTAAATACT 
ATAATGGAGA TTATTACTGA AGCTGGCCCA GTAAGCAATG AAGGAAACAG GAGAGCCCTG 
GTGGATCAAA AGTCTTCAGT CATTAAGCAT AGCCCAACAG TGAAGAGGGA ATCTCCATCA 
CCTCAGGGAC GAAGTAGTAA CTCCAGTGAG AACCAGCAAT TCCTGAAGGA GGTGGTACAC 
AATGTTCTCG ATGGGCAAGG TGTTGGTTGG CTGAACATGA AGAGAGTCCG ACGTCTGCTG 
GAGAGTGAGC AGCTCCGTGT CTTTGTACTA AGCAAGCTAA ATCGCACGAT CCAGTCGGAA 
GAAGATGCTC AGCAGGATAT CATACAGGAT GTGGAGATCA GCCGCAAAGT TTATAAAGGC 
ATGCTGGACT TGCTAAAGTG TACTGTGCTG AGCCTGGAGC AGTCATACGC AAATGCTGGC 
CTGGGAGGCA TGGCCAGTGT TTTTGGCCTT CTAGAAATAG CACATACTCA CTATTACAAC 
AAAGAACCAG AAAAGAGAAA ACGAAGCCCA ACAGATGGAT CTGTCACTCC GGTTGGCAAA 
GATCCTGCAT CATCTCCAAG AGTGGAGCCG AAACCTGCCA TGCAGCTGCC AGTACCACAG 
CTGATGCCGA AGGCACCAAG CCCTGCAGGG AAAGGGCTAA GGGAGTTTGA TACAAGGAGT 
CTAAAGGAAG AAAATTTTAT TGCTTCCATT GAATTGTGGA ACAAGCACCA GGAAGTGAAA 
AAGCAAAAAT CTTTGGAAAA AACGAGGCCA GAAGGTGTGA AACATCTTGA TTTGGGAGAA 
ACAGATGAGA AAAAATCCCA AATCAGTGCA GACAGTGGCC TCAGTTTGGC CTCAGGTTCT 
CAGAAGAGTG ATTTTGACTC TGTTCCCAGT GGAGGACCGT CAGTTATGGT CCGAAGTACC 
AGCCAGGATT CTGAAGTTAG CACAGTGGTT AGTAACAGCT CTGGGGAGAC ACTAGGAGCA 
GACAGTGACT TGAGTAGCAA TGCTGGTGAT GGCCCAAGTG TGGAAAATGG AGGCAATCTG 
GCAGGATCCA GGGGCACCGT GTCAGACAGT GAAATTGAGA CAAACTCTGC TACTAGCTCT 
ATTTTCGCAA AGTCTCATAA CTTGAAGCAG AATGTAAAGG AGAACAAAGG CAGTACTGCA 
GGGAAAGGTC CAGAGGATGG GAACCAGCGT GTCTATCTCT ATGAAGGACT TTTGGGTAGG 
GATAAAGGAT CTGTCTGGGA CCAGTTAGAG GATGCCGCAA TGGAAACCTT CTCTATGAGC 
AAAGAGCGCT CAACACTGTG GGACCAGATG CAATTCTGGG AAGATGCCTT TTTGGATGCT 
GTGATGTTAG AGAGAGAAGG AATGGGGATG GATCAGGGAC CCCAGGAGAT GATTGACAGG 
TATCTTTCCC TGGGAGAACA TGATCGAAAA CGTTTGGAGG ATGATGAGGA CCGCTTATTG 
GCTACGCTGC TACATAATAT GATTGCTTAT ATGCTTATGA TAAAGGTGAA TAAGAATGAT 
ATTAGGAAAA AGGTGCGTCG TCTGATGGGA AAATCGCATA TTGGATTGTT GCACAGCCAG 
CAGTTAAATG ACATCCTAGA CAAGCTTGCT AACCTGAATG GACGGGAACT TCCTGTGAGA 
CCCAGCGGCA GCCGCCACAT CAAGAAGCAG ACTTTTGTAG TCCATGCTGG GACAGACACT 
ACAGGAGATA TATTTTTCAT GGAGGTTTGT GATGACTGCA TTGTGCTGAG AAGCAACATT 
GGAACTGTGT ATGAACGTTG GTGGTATGAG AAACTCATCA ACATGACTTA CTGTCCCAAA 
ACAAAAGTTC TGTGTCTCTG GCGGAGGAAT GGTCAGGAAA CACAACTGAA CAAGTTCTAC 
ACAAAGAAGT GTCGGGAATT ATACTACTGC GTAAAAGACA GCATGGAGCG AGCAGCAGCA 
AGACAGCAAA GCATTAAGCC AGGCCCTGAG TTGGGTGGTG AGTTTCCTGT GCAGGATATG 
AAAACTGGCG AAGGAGGTCT TCTGCAGGTG ACACTGGAAG GAATTAACCT GAAATTTATG 
CACAGTCAGG AGCGGAAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACTGTG 
CGAGGAGTCT TTGTCCTGGA GGAATTTGTT CCTGAAACTA AAGAAGTGGT GAGCCACAAG 
TACAAGACGC CAATGGCTCA CGAGATCTGC TACTCCGTGC TGTGTCTCTT CTCTTATGTG 
GCTGCCATTC GTGGGAAAGA GGCAGAAAAC AAAAGCAAAC CACCTCGGCC AGTTTCAAGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287162.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142648.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X9, mRNA.

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CloneID	OGa36679
Clone ID Related Accession (Same CDS sequence)	XM_015287162.2 , XM_015287162.1
Accession Version	XM_015287162.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287162.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACTGTGCGA 
GGAGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAGTAC 
AAGACGCCAA TGGCTCACGA GATCTGCTAC TCCGTGCTGT GTCTCTTCTC TTATGTGGCT 
GCCATTCGTG GGAAAGAGGC AGAAAACAAA AGCAAACCAC CTCGGCCAGT TTCAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142648.1
CDS	277..5316
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKDYFNLEVGRDVNEVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287162.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287162.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACTGTGCGA 
GGAGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAGTAC 
AAGACGCCAA TGGCTCACGA GATCTGCTAC TCCGTGCTGT GTCTCTTCTC TTATGTGGCT 
GCCATTCGTG GGAAAGAGGC AGAAAACAAA AGCAAACCAC CTCGGCCAGT TTCAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287162.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142648.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X9, mRNA.

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CloneID	OGa36679
Clone ID Related Accession (Same CDS sequence)	XM_015287162.2 , XM_015287162.1
Accession Version	XM_015287162.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACTGTGCGA 
GGAGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAGTAC 
AAGACGCCAA TGGCTCACGA GATCTGCTAC TCCGTGCTGT GTCTCTTCTC TTATGTGGCT 
GCCATTCGTG GGAAAGAGGC AGAAAACAAA AGCAAACCAC CTCGGCCAGT TTCAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142648.1
CDS	233..5272
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKDYFNLEVGRDVNEVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287162.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287162.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACTGTGCGA 
GGAGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAGTAC 
AAGACGCCAA TGGCTCACGA GATCTGCTAC TCCGTGCTGT GTCTCTTCTC TTATGTGGCT 
GCCATTCGTG GGAAAGAGGC AGAAAACAAA AGCAAACCAC CTCGGCCAGT TTCAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287163.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142649.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X10, mRNA.

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CloneID	OGa36680
Clone ID Related Accession (Same CDS sequence)	XM_015287163.1 , XM_015287163.2
Accession Version	XM_015287163.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACTGTGCGA 
GGAGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAGTAC 
AAGACGCCAA TGGCTCACGA GATCTGCTAC TCCGTGCTGT GTCTCTTCTC TTATGTGGCT 
GCCATTCGTG GGAAAGAGGC AGAAAACAAA AGCAAACCAC CTCGGCCAGT TTCAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142649.1
CDS	233..5272
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKDYFNLEVGRDVNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287163.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287163.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACTGTGCGA 
GGAGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAGTAC 
AAGACGCCAA TGGCTCACGA GATCTGCTAC TCCGTGCTGT GTCTCTTCTC TTATGTGGCT 
GCCATTCGTG GGAAAGAGGC AGAAAACAAA AGCAAACCAC CTCGGCCAGT TTCAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287163.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142649.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X10, mRNA.

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CloneID	OGa36680
Clone ID Related Accession (Same CDS sequence)	XM_015287163.1 , XM_015287163.2
Accession Version	XM_015287163.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287163.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACTGTGCGA 
GGAGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAGTAC 
AAGACGCCAA TGGCTCACGA GATCTGCTAC TCCGTGCTGT GTCTCTTCTC TTATGTGGCT 
GCCATTCGTG GGAAAGAGGC AGAAAACAAA AGCAAACCAC CTCGGCCAGT TTCAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142649.1
CDS	277..5316
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKDYFNLEVGRDVNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287163.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287163.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACTGTGCGA 
GGAGTCTTTG TCCTGGAGGA ATTTGTTCCT GAAACTAAAG AAGTGGTGAG CCACAAGTAC 
AAGACGCCAA TGGCTCACGA GATCTGCTAC TCCGTGCTGT GTCTCTTCTC TTATGTGGCT 
GCCATTCGTG GGAAAGAGGC AGAAAACAAA AGCAAACCAC CTCGGCCAGT TTCAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287165.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142651.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OGa36681
Clone ID Related Accession (Same CDS sequence)	XM_015287165.2 , XM_015287165.1
Accession Version	XM_015287165.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287165.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGCAAAGAG 
CGCTCAACAC TGTGGGACCA GATGCAATTC TGGGAAGATG CCTTTTTGGA TGCTGTGATG 
TTAGAGAGAG AAGGAATGGG GATGGATCAG GGACCCCAGG AGATGATTGA CAGGTATCTT 
TCCCTGGGAG AACATGATCG AAAACGTTTG GAGGATGATG AGGACCGCTT ATTGGCTACG 
CTGCTACATA ATATGATTGC TTATATGCTT ATGATAAAGG TGAATAAGAA TGATATTAGG 
AAAAAGGTGC GTCGTCTGAT GGGAAAATCG CATATTGGAT TGTTGCACAG CCAGCAGTTA 
AATGACATCC TAGACAAGCT TGCTAACCTG AATGGACGGG AACTTCCTGT GAGACCCAGC 
GGCAGCCGCC ACATCAAGAA GCAGACTTTT GTAGTCCATG CTGGGACAGA CACTACAGGA 
GATATATTTT TCATGGAGGT TTGTGATGAC TGCATTGTGC TGAGAAGCAA CATTGGAACT 
GTGTATGAAC GTTGGTGGTA TGAGAAACTC ATCAACATGA CTTACTGTCC CAAAACAAAA 
GTTCTGTGTC TCTGGCGGAG GAATGGTCAG GAAACACAAC TGAACAAGTT CTACACAAAG 
AAGTGTCGGG AATTATACTA CTGCGTAAAA GACAGCATGG AGCGAGCAGC AGCAAGACAG 
CAAAGCATTA AGCCAGGCCC TGAGTTGGGT GGTGAGTTTC CTGTGCAGGA TATGAAAACT 
GGCGAAGGAG GTCTTCTGCA GGTGACACTG GAAGGAATTA ACCTGAAATT TATGCACAGT 
CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT CACATTAAAA AGTGCAATAC TGTGCGAGGA 
GTCTTTGTCC TGGAGGAATT TGTTCCTGAA ACTAAAGAAG TGGTGAGCCA CAAGTACAAG 
ACGCCAATGG CTCACGAGAT CTGCTACTCC GTGCTGTGTC TCTTCTCTTA TGTGGCTGCC 
ATTCGTGGGA AAGAGGCAGA AAACAAAAGC AAACCACCTC GGCCAGTTTC AAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142651.1
CDS	277..5313
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKDYFNLEVGRDVNEVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287165.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287165.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGCAAAGAG 
CGCTCAACAC TGTGGGACCA GATGCAATTC TGGGAAGATG CCTTTTTGGA TGCTGTGATG 
TTAGAGAGAG AAGGAATGGG GATGGATCAG GGACCCCAGG AGATGATTGA CAGGTATCTT 
TCCCTGGGAG AACATGATCG AAAACGTTTG GAGGATGATG AGGACCGCTT ATTGGCTACG 
CTGCTACATA ATATGATTGC TTATATGCTT ATGATAAAGG TGAATAAGAA TGATATTAGG 
AAAAAGGTGC GTCGTCTGAT GGGAAAATCG CATATTGGAT TGTTGCACAG CCAGCAGTTA 
AATGACATCC TAGACAAGCT TGCTAACCTG AATGGACGGG AACTTCCTGT GAGACCCAGC 
GGCAGCCGCC ACATCAAGAA GCAGACTTTT GTAGTCCATG CTGGGACAGA CACTACAGGA 
GATATATTTT TCATGGAGGT TTGTGATGAC TGCATTGTGC TGAGAAGCAA CATTGGAACT 
GTGTATGAAC GTTGGTGGTA TGAGAAACTC ATCAACATGA CTTACTGTCC CAAAACAAAA 
GTTCTGTGTC TCTGGCGGAG GAATGGTCAG GAAACACAAC TGAACAAGTT CTACACAAAG 
AAGTGTCGGG AATTATACTA CTGCGTAAAA GACAGCATGG AGCGAGCAGC AGCAAGACAG 
CAAAGCATTA AGCCAGGCCC TGAGTTGGGT GGTGAGTTTC CTGTGCAGGA TATGAAAACT 
GGCGAAGGAG GTCTTCTGCA GGTGACACTG GAAGGAATTA ACCTGAAATT TATGCACAGT 
CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT CACATTAAAA AGTGCAATAC TGTGCGAGGA 
GTCTTTGTCC TGGAGGAATT TGTTCCTGAA ACTAAAGAAG TGGTGAGCCA CAAGTACAAG 
ACGCCAATGG CTCACGAGAT CTGCTACTCC GTGCTGTGTC TCTTCTCTTA TGTGGCTGCC 
ATTCGTGGGA AAGAGGCAGA AAACAAAAGC AAACCACCTC GGCCAGTTTC AAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287165.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142651.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X11, mRNA.

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CloneID	OGa36681
Clone ID Related Accession (Same CDS sequence)	XM_015287165.2 , XM_015287165.1
Accession Version	XM_015287165.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGCAAAGAG 
CGCTCAACAC TGTGGGACCA GATGCAATTC TGGGAAGATG CCTTTTTGGA TGCTGTGATG 
TTAGAGAGAG AAGGAATGGG GATGGATCAG GGACCCCAGG AGATGATTGA CAGGTATCTT 
TCCCTGGGAG AACATGATCG AAAACGTTTG GAGGATGATG AGGACCGCTT ATTGGCTACG 
CTGCTACATA ATATGATTGC TTATATGCTT ATGATAAAGG TGAATAAGAA TGATATTAGG 
AAAAAGGTGC GTCGTCTGAT GGGAAAATCG CATATTGGAT TGTTGCACAG CCAGCAGTTA 
AATGACATCC TAGACAAGCT TGCTAACCTG AATGGACGGG AACTTCCTGT GAGACCCAGC 
GGCAGCCGCC ACATCAAGAA GCAGACTTTT GTAGTCCATG CTGGGACAGA CACTACAGGA 
GATATATTTT TCATGGAGGT TTGTGATGAC TGCATTGTGC TGAGAAGCAA CATTGGAACT 
GTGTATGAAC GTTGGTGGTA TGAGAAACTC ATCAACATGA CTTACTGTCC CAAAACAAAA 
GTTCTGTGTC TCTGGCGGAG GAATGGTCAG GAAACACAAC TGAACAAGTT CTACACAAAG 
AAGTGTCGGG AATTATACTA CTGCGTAAAA GACAGCATGG AGCGAGCAGC AGCAAGACAG 
CAAAGCATTA AGCCAGGCCC TGAGTTGGGT GGTGAGTTTC CTGTGCAGGA TATGAAAACT 
GGCGAAGGAG GTCTTCTGCA GGTGACACTG GAAGGAATTA ACCTGAAATT TATGCACAGT 
CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT CACATTAAAA AGTGCAATAC TGTGCGAGGA 
GTCTTTGTCC TGGAGGAATT TGTTCCTGAA ACTAAAGAAG TGGTGAGCCA CAAGTACAAG 
ACGCCAATGG CTCACGAGAT CTGCTACTCC GTGCTGTGTC TCTTCTCTTA TGTGGCTGCC 
ATTCGTGGGA AAGAGGCAGA AAACAAAAGC AAACCACCTC GGCCAGTTTC AAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142651.1
CDS	233..5269
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKDYFNLEVGRDVNEVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287165.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287165.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGCAAAGAG 
CGCTCAACAC TGTGGGACCA GATGCAATTC TGGGAAGATG CCTTTTTGGA TGCTGTGATG 
TTAGAGAGAG AAGGAATGGG GATGGATCAG GGACCCCAGG AGATGATTGA CAGGTATCTT 
TCCCTGGGAG AACATGATCG AAAACGTTTG GAGGATGATG AGGACCGCTT ATTGGCTACG 
CTGCTACATA ATATGATTGC TTATATGCTT ATGATAAAGG TGAATAAGAA TGATATTAGG 
AAAAAGGTGC GTCGTCTGAT GGGAAAATCG CATATTGGAT TGTTGCACAG CCAGCAGTTA 
AATGACATCC TAGACAAGCT TGCTAACCTG AATGGACGGG AACTTCCTGT GAGACCCAGC 
GGCAGCCGCC ACATCAAGAA GCAGACTTTT GTAGTCCATG CTGGGACAGA CACTACAGGA 
GATATATTTT TCATGGAGGT TTGTGATGAC TGCATTGTGC TGAGAAGCAA CATTGGAACT 
GTGTATGAAC GTTGGTGGTA TGAGAAACTC ATCAACATGA CTTACTGTCC CAAAACAAAA 
GTTCTGTGTC TCTGGCGGAG GAATGGTCAG GAAACACAAC TGAACAAGTT CTACACAAAG 
AAGTGTCGGG AATTATACTA CTGCGTAAAA GACAGCATGG AGCGAGCAGC AGCAAGACAG 
CAAAGCATTA AGCCAGGCCC TGAGTTGGGT GGTGAGTTTC CTGTGCAGGA TATGAAAACT 
GGCGAAGGAG GTCTTCTGCA GGTGACACTG GAAGGAATTA ACCTGAAATT TATGCACAGT 
CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT CACATTAAAA AGTGCAATAC TGTGCGAGGA 
GTCTTTGTCC TGGAGGAATT TGTTCCTGAA ACTAAAGAAG TGGTGAGCCA CAAGTACAAG 
ACGCCAATGG CTCACGAGAT CTGCTACTCC GTGCTGTGTC TCTTCTCTTA TGTGGCTGCC 
ATTCGTGGGA AAGAGGCAGA AAACAAAAGC AAACCACCTC GGCCAGTTTC AAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287166.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142652.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X12, mRNA.

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CloneID	OGa36682
Clone ID Related Accession (Same CDS sequence)	XM_015287166.2 , XM_015287166.1
Accession Version	XM_015287166.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287166.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGCAAAGAG 
CGCTCAACAC TGTGGGACCA GATGCAATTC TGGGAAGATG CCTTTTTGGA TGCTGTGATG 
TTAGAGAGAG AAGGAATGGG GATGGATCAG GGACCCCAGG AGATGATTGA CAGGTATCTT 
TCCCTGGGAG AACATGATCG AAAACGTTTG GAGGATGATG AGGACCGCTT ATTGGCTACG 
CTGCTACATA ATATGATTGC TTATATGCTT ATGATAAAGG TGAATAAGAA TGATATTAGG 
AAAAAGGTGC GTCGTCTGAT GGGAAAATCG CATATTGGAT TGTTGCACAG CCAGCAGTTA 
AATGACATCC TAGACAAGCT TGCTAACCTG AATGGACGGG AACTTCCTGT GAGACCCAGC 
GGCAGCCGCC ACATCAAGAA GCAGACTTTT GTAGTCCATG CTGGGACAGA CACTACAGGA 
GATATATTTT TCATGGAGGT TTGTGATGAC TGCATTGTGC TGAGAAGCAA CATTGGAACT 
GTGTATGAAC GTTGGTGGTA TGAGAAACTC ATCAACATGA CTTACTGTCC CAAAACAAAA 
GTTCTGTGTC TCTGGCGGAG GAATGGTCAG GAAACACAAC TGAACAAGTT CTACACAAAG 
AAGTGTCGGG AATTATACTA CTGCGTAAAA GACAGCATGG AGCGAGCAGC AGCAAGACAG 
CAAAGCATTA AGCCAGGCCC TGAGTTGGGT GGTGAGTTTC CTGTGCAGGA TATGAAAACT 
GGCGAAGGAG GTCTTCTGCA GGTGACACTG GAAGGAATTA ACCTGAAATT TATGCACAGT 
CAGGTTTTCA TAGAGCTGAA TCACATTAAA AAGTGCAATA CTGTGCGAGG AGTCTTTGTC 
CTGGAGGAAT TTGTTCCTGA AACTAAAGAA GTGGTGAGCC ACAAGTACAA GACGCCAATG 
GCTCACGAGA TCTGCTACTC CGTGCTGTGT CTCTTCTCTT ATGTGGCTGC CATTCGTGGG 
AAAGAGGCAG AAAACAAAAG CAAACCACCT CGGCCAGTTT CAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142652.1
CDS	277..5304
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKDYFNLEVGRDVNEVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287166.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287166.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGCAAAGAG 
CGCTCAACAC TGTGGGACCA GATGCAATTC TGGGAAGATG CCTTTTTGGA TGCTGTGATG 
TTAGAGAGAG AAGGAATGGG GATGGATCAG GGACCCCAGG AGATGATTGA CAGGTATCTT 
TCCCTGGGAG AACATGATCG AAAACGTTTG GAGGATGATG AGGACCGCTT ATTGGCTACG 
CTGCTACATA ATATGATTGC TTATATGCTT ATGATAAAGG TGAATAAGAA TGATATTAGG 
AAAAAGGTGC GTCGTCTGAT GGGAAAATCG CATATTGGAT TGTTGCACAG CCAGCAGTTA 
AATGACATCC TAGACAAGCT TGCTAACCTG AATGGACGGG AACTTCCTGT GAGACCCAGC 
GGCAGCCGCC ACATCAAGAA GCAGACTTTT GTAGTCCATG CTGGGACAGA CACTACAGGA 
GATATATTTT TCATGGAGGT TTGTGATGAC TGCATTGTGC TGAGAAGCAA CATTGGAACT 
GTGTATGAAC GTTGGTGGTA TGAGAAACTC ATCAACATGA CTTACTGTCC CAAAACAAAA 
GTTCTGTGTC TCTGGCGGAG GAATGGTCAG GAAACACAAC TGAACAAGTT CTACACAAAG 
AAGTGTCGGG AATTATACTA CTGCGTAAAA GACAGCATGG AGCGAGCAGC AGCAAGACAG 
CAAAGCATTA AGCCAGGCCC TGAGTTGGGT GGTGAGTTTC CTGTGCAGGA TATGAAAACT 
GGCGAAGGAG GTCTTCTGCA GGTGACACTG GAAGGAATTA ACCTGAAATT TATGCACAGT 
CAGGTTTTCA TAGAGCTGAA TCACATTAAA AAGTGCAATA CTGTGCGAGG AGTCTTTGTC 
CTGGAGGAAT TTGTTCCTGA AACTAAAGAA GTGGTGAGCC ACAAGTACAA GACGCCAATG 
GCTCACGAGA TCTGCTACTC CGTGCTGTGT CTCTTCTCTT ATGTGGCTGC CATTCGTGGG 
AAAGAGGCAG AAAACAAAAG CAAACCACCT CGGCCAGTTT CAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287166.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142652.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X12, mRNA.

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CloneID	OGa36682
Clone ID Related Accession (Same CDS sequence)	XM_015287166.2 , XM_015287166.1
Accession Version	XM_015287166.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGCAAAGAG 
CGCTCAACAC TGTGGGACCA GATGCAATTC TGGGAAGATG CCTTTTTGGA TGCTGTGATG 
TTAGAGAGAG AAGGAATGGG GATGGATCAG GGACCCCAGG AGATGATTGA CAGGTATCTT 
TCCCTGGGAG AACATGATCG AAAACGTTTG GAGGATGATG AGGACCGCTT ATTGGCTACG 
CTGCTACATA ATATGATTGC TTATATGCTT ATGATAAAGG TGAATAAGAA TGATATTAGG 
AAAAAGGTGC GTCGTCTGAT GGGAAAATCG CATATTGGAT TGTTGCACAG CCAGCAGTTA 
AATGACATCC TAGACAAGCT TGCTAACCTG AATGGACGGG AACTTCCTGT GAGACCCAGC 
GGCAGCCGCC ACATCAAGAA GCAGACTTTT GTAGTCCATG CTGGGACAGA CACTACAGGA 
GATATATTTT TCATGGAGGT TTGTGATGAC TGCATTGTGC TGAGAAGCAA CATTGGAACT 
GTGTATGAAC GTTGGTGGTA TGAGAAACTC ATCAACATGA CTTACTGTCC CAAAACAAAA 
GTTCTGTGTC TCTGGCGGAG GAATGGTCAG GAAACACAAC TGAACAAGTT CTACACAAAG 
AAGTGTCGGG AATTATACTA CTGCGTAAAA GACAGCATGG AGCGAGCAGC AGCAAGACAG 
CAAAGCATTA AGCCAGGCCC TGAGTTGGGT GGTGAGTTTC CTGTGCAGGA TATGAAAACT 
GGCGAAGGAG GTCTTCTGCA GGTGACACTG GAAGGAATTA ACCTGAAATT TATGCACAGT 
CAGGTTTTCA TAGAGCTGAA TCACATTAAA AAGTGCAATA CTGTGCGAGG AGTCTTTGTC 
CTGGAGGAAT TTGTTCCTGA AACTAAAGAA GTGGTGAGCC ACAAGTACAA GACGCCAATG 
GCTCACGAGA TCTGCTACTC CGTGCTGTGT CTCTTCTCTT ATGTGGCTGC CATTCGTGGG 
AAAGAGGCAG AAAACAAAAG CAAACCACCT CGGCCAGTTT CAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142652.1
CDS	233..5260
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKDYFNLEVGRDVNEVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287166.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287166.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGCAAAGAG 
CGCTCAACAC TGTGGGACCA GATGCAATTC TGGGAAGATG CCTTTTTGGA TGCTGTGATG 
TTAGAGAGAG AAGGAATGGG GATGGATCAG GGACCCCAGG AGATGATTGA CAGGTATCTT 
TCCCTGGGAG AACATGATCG AAAACGTTTG GAGGATGATG AGGACCGCTT ATTGGCTACG 
CTGCTACATA ATATGATTGC TTATATGCTT ATGATAAAGG TGAATAAGAA TGATATTAGG 
AAAAAGGTGC GTCGTCTGAT GGGAAAATCG CATATTGGAT TGTTGCACAG CCAGCAGTTA 
AATGACATCC TAGACAAGCT TGCTAACCTG AATGGACGGG AACTTCCTGT GAGACCCAGC 
GGCAGCCGCC ACATCAAGAA GCAGACTTTT GTAGTCCATG CTGGGACAGA CACTACAGGA 
GATATATTTT TCATGGAGGT TTGTGATGAC TGCATTGTGC TGAGAAGCAA CATTGGAACT 
GTGTATGAAC GTTGGTGGTA TGAGAAACTC ATCAACATGA CTTACTGTCC CAAAACAAAA 
GTTCTGTGTC TCTGGCGGAG GAATGGTCAG GAAACACAAC TGAACAAGTT CTACACAAAG 
AAGTGTCGGG AATTATACTA CTGCGTAAAA GACAGCATGG AGCGAGCAGC AGCAAGACAG 
CAAAGCATTA AGCCAGGCCC TGAGTTGGGT GGTGAGTTTC CTGTGCAGGA TATGAAAACT 
GGCGAAGGAG GTCTTCTGCA GGTGACACTG GAAGGAATTA ACCTGAAATT TATGCACAGT 
CAGGTTTTCA TAGAGCTGAA TCACATTAAA AAGTGCAATA CTGTGCGAGG AGTCTTTGTC 
CTGGAGGAAT TTGTTCCTGA AACTAAAGAA GTGGTGAGCC ACAAGTACAA GACGCCAATG 
GCTCACGAGA TCTGCTACTC CGTGCTGTGT CTCTTCTCTT ATGTGGCTGC CATTCGTGGG 
AAAGAGGCAG AAAACAAAAG CAAACCACCT CGGCCAGTTT CAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287170.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142656.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X16, mRNA.

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CloneID	OGa36683
Clone ID Related Accession (Same CDS sequence)	XM_015287170.1 , XM_015287170.2
Accession Version	XM_015287170.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG TAGGGATAAA GGATCTGTCT GGGACCAGTT AGAGGATGCC 
GCAATGGAAA CCTTCTCTAT GAGCAAAGAG CGCTCAACAC TGTGGGACCA GATGCAATTC 
TGGGAAGATG CCTTTTTGGA TGCTGTGATG TTAGAGAGAG AAGGAATGGG GATGGATCAG 
GGACCCCAGG AGATGATTGA CAGGTATCTT TCCCTGGGAG AACATGATCG AAAACGTTTG 
GAGGATGATG AGGACCGCTT ATTGGCTACG CTGCTACATA ATATGATTGC TTATATGCTT 
ATGATAAAGG TGAATAAGAA TGATATTAGG AAAAAGGTGC GTCGTCTGAT GGGAAAATCG 
CATATTGGAT TGTTGCACAG CCAGCAGTTA AATGACATCC TAGACAAGCT TGCTAACCTG 
AATGGACGGG AACTTCCTGT GAGACCCAGC GGCAGCCGCC ACATCAAGAA GCAGACTTTT 
GTAGTCCATG CTGGGACAGA CACTACAGGA GATATATTTT TCATGGAGGT TTGTGATGAC 
TGCATTGTGC TGAGAAGCAA CATTGGAACT GTGTATGAAC GTTGGTGGTA TGAGAAACTC 
ATCAACATGA CTTACTGTCC CAAAACAAAA GTTCTGTGTC TCTGGCGGAG GAATGGTCAG 
GAAACACAAC TGAACAAGTT CTACACAAAG AAGTGTCGGG AATTATACTA CTGCGTAAAA 
GACAGCATGG AGCGAGCAGC AGCAAGACAG CAAAGCATTA AGCCAGGCCC TGAGTTGGGT 
GGTGAGTTTC CTGTGCAGGA TATGAAAACT GGCGAAGGAG GTCTTCTGCA GGTGACACTG 
GAAGGAATTA ACCTGAAATT TATGCACAGT CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC TGTGCGAGGA GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ACTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCAATGG CTCACGAGAT CTGCTACTCC 
GTGCTGTGTC TCTTCTCTTA TGTGGCTGCC ATTCGTGGGA AAGAGGCAGA AAACAAAAGC 
AAACCACCTC GGCCAGTTTC AAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142656.1
CDS	233..5179
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIGPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287170.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287170.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG TAGGGATAAA GGATCTGTCT GGGACCAGTT AGAGGATGCC 
GCAATGGAAA CCTTCTCTAT GAGCAAAGAG CGCTCAACAC TGTGGGACCA GATGCAATTC 
TGGGAAGATG CCTTTTTGGA TGCTGTGATG TTAGAGAGAG AAGGAATGGG GATGGATCAG 
GGACCCCAGG AGATGATTGA CAGGTATCTT TCCCTGGGAG AACATGATCG AAAACGTTTG 
GAGGATGATG AGGACCGCTT ATTGGCTACG CTGCTACATA ATATGATTGC TTATATGCTT 
ATGATAAAGG TGAATAAGAA TGATATTAGG AAAAAGGTGC GTCGTCTGAT GGGAAAATCG 
CATATTGGAT TGTTGCACAG CCAGCAGTTA AATGACATCC TAGACAAGCT TGCTAACCTG 
AATGGACGGG AACTTCCTGT GAGACCCAGC GGCAGCCGCC ACATCAAGAA GCAGACTTTT 
GTAGTCCATG CTGGGACAGA CACTACAGGA GATATATTTT TCATGGAGGT TTGTGATGAC 
TGCATTGTGC TGAGAAGCAA CATTGGAACT GTGTATGAAC GTTGGTGGTA TGAGAAACTC 
ATCAACATGA CTTACTGTCC CAAAACAAAA GTTCTGTGTC TCTGGCGGAG GAATGGTCAG 
GAAACACAAC TGAACAAGTT CTACACAAAG AAGTGTCGGG AATTATACTA CTGCGTAAAA 
GACAGCATGG AGCGAGCAGC AGCAAGACAG CAAAGCATTA AGCCAGGCCC TGAGTTGGGT 
GGTGAGTTTC CTGTGCAGGA TATGAAAACT GGCGAAGGAG GTCTTCTGCA GGTGACACTG 
GAAGGAATTA ACCTGAAATT TATGCACAGT CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC TGTGCGAGGA GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ACTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCAATGG CTCACGAGAT CTGCTACTCC 
GTGCTGTGTC TCTTCTCTTA TGTGGCTGCC ATTCGTGGGA AAGAGGCAGA AAACAAAAGC 
AAACCACCTC GGCCAGTTTC AAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287170.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142656.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X16, mRNA.

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CloneID	OGa36683
Clone ID Related Accession (Same CDS sequence)	XM_015287170.1 , XM_015287170.2
Accession Version	XM_015287170.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287170.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG TAGGGATAAA GGATCTGTCT GGGACCAGTT AGAGGATGCC 
GCAATGGAAA CCTTCTCTAT GAGCAAAGAG CGCTCAACAC TGTGGGACCA GATGCAATTC 
TGGGAAGATG CCTTTTTGGA TGCTGTGATG TTAGAGAGAG AAGGAATGGG GATGGATCAG 
GGACCCCAGG AGATGATTGA CAGGTATCTT TCCCTGGGAG AACATGATCG AAAACGTTTG 
GAGGATGATG AGGACCGCTT ATTGGCTACG CTGCTACATA ATATGATTGC TTATATGCTT 
ATGATAAAGG TGAATAAGAA TGATATTAGG AAAAAGGTGC GTCGTCTGAT GGGAAAATCG 
CATATTGGAT TGTTGCACAG CCAGCAGTTA AATGACATCC TAGACAAGCT TGCTAACCTG 
AATGGACGGG AACTTCCTGT GAGACCCAGC GGCAGCCGCC ACATCAAGAA GCAGACTTTT 
GTAGTCCATG CTGGGACAGA CACTACAGGA GATATATTTT TCATGGAGGT TTGTGATGAC 
TGCATTGTGC TGAGAAGCAA CATTGGAACT GTGTATGAAC GTTGGTGGTA TGAGAAACTC 
ATCAACATGA CTTACTGTCC CAAAACAAAA GTTCTGTGTC TCTGGCGGAG GAATGGTCAG 
GAAACACAAC TGAACAAGTT CTACACAAAG AAGTGTCGGG AATTATACTA CTGCGTAAAA 
GACAGCATGG AGCGAGCAGC AGCAAGACAG CAAAGCATTA AGCCAGGCCC TGAGTTGGGT 
GGTGAGTTTC CTGTGCAGGA TATGAAAACT GGCGAAGGAG GTCTTCTGCA GGTGACACTG 
GAAGGAATTA ACCTGAAATT TATGCACAGT CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC TGTGCGAGGA GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ACTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCAATGG CTCACGAGAT CTGCTACTCC 
GTGCTGTGTC TCTTCTCTTA TGTGGCTGCC ATTCGTGGGA AAGAGGCAGA AAACAAAAGC 
AAACCACCTC GGCCAGTTTC AAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142656.1
CDS	277..5223
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIGPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287170.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287170.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG TAGGGATAAA GGATCTGTCT GGGACCAGTT AGAGGATGCC 
GCAATGGAAA CCTTCTCTAT GAGCAAAGAG CGCTCAACAC TGTGGGACCA GATGCAATTC 
TGGGAAGATG CCTTTTTGGA TGCTGTGATG TTAGAGAGAG AAGGAATGGG GATGGATCAG 
GGACCCCAGG AGATGATTGA CAGGTATCTT TCCCTGGGAG AACATGATCG AAAACGTTTG 
GAGGATGATG AGGACCGCTT ATTGGCTACG CTGCTACATA ATATGATTGC TTATATGCTT 
ATGATAAAGG TGAATAAGAA TGATATTAGG AAAAAGGTGC GTCGTCTGAT GGGAAAATCG 
CATATTGGAT TGTTGCACAG CCAGCAGTTA AATGACATCC TAGACAAGCT TGCTAACCTG 
AATGGACGGG AACTTCCTGT GAGACCCAGC GGCAGCCGCC ACATCAAGAA GCAGACTTTT 
GTAGTCCATG CTGGGACAGA CACTACAGGA GATATATTTT TCATGGAGGT TTGTGATGAC 
TGCATTGTGC TGAGAAGCAA CATTGGAACT GTGTATGAAC GTTGGTGGTA TGAGAAACTC 
ATCAACATGA CTTACTGTCC CAAAACAAAA GTTCTGTGTC TCTGGCGGAG GAATGGTCAG 
GAAACACAAC TGAACAAGTT CTACACAAAG AAGTGTCGGG AATTATACTA CTGCGTAAAA 
GACAGCATGG AGCGAGCAGC AGCAAGACAG CAAAGCATTA AGCCAGGCCC TGAGTTGGGT 
GGTGAGTTTC CTGTGCAGGA TATGAAAACT GGCGAAGGAG GTCTTCTGCA GGTGACACTG 
GAAGGAATTA ACCTGAAATT TATGCACAGT CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC TGTGCGAGGA GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ACTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCAATGG CTCACGAGAT CTGCTACTCC 
GTGCTGTGTC TCTTCTCTTA TGTGGCTGCC ATTCGTGGGA AAGAGGCAGA AAACAAAAGC 
AAACCACCTC GGCCAGTTTC AAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287173.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142659.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OGa36684
Clone ID Related Accession (Same CDS sequence)	XM_015287173.1 , XM_015287173.2
Accession Version	XM_015287173.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGTTCC TGAAACTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142659.1
CDS	233..5122
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKDYFNLEVGRDVNEVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_015287173.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287173.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGTTCC TGAAACTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287173.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142659.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OGa36684
Clone ID Related Accession (Same CDS sequence)	XM_015287173.1 , XM_015287173.2
Accession Version	XM_015287173.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287173.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGTTCC TGAAACTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142659.1
CDS	277..5166
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKDYFNLEVGRDVNEVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_015287173.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287173.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGTTCC TGAAACTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287174.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142660.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X20, mRNA.

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CloneID	OGa36685
Clone ID Related Accession (Same CDS sequence)	XM_015287174.1 , XM_015287174.2
Accession Version	XM_015287174.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG TAGGGATAAA GGATCTGTCT GGGACCAGTT AGAGGATGCC 
GCAATGGAAA CCTTCTCTAT GAGCAAAGAG CGCTCAACAC TGTGGGACCA GATGCAATTC 
TGGGAAGATG CCTTTTTGGA TGCTGTGATG TTAGAGAGAG AAGGAATGGG GATGGATCAG 
GGACCCCAGG AGATGATTGA CAGGTATCTT TCCCTGGGAG AACATGATCG AAAACGTTTG 
GAGGATGATG AGGACCGCTT ATTGGCTACG CTGCTACATA ATATGATTGC TTATATGCTT 
ATGATAAAGG TGAATAAGAA TGATATTAGG AAAAAGGTGC GTCGTCTGAT GGGAAAATCG 
CATATTGGAT TGTTGCACAG CCAGCAGTTA AATGACATCC TAGACAAGCT TGCTAACCTG 
AATGGACGGG AACTTCCTGT GAGACCCAGC GGCAGCCGCC ACATCAAGAA GCAGACTTTT 
GTAGTCCATG CTGGGACAGA CACTACAGGA GATATATTTT TCATGGAGGT TTGTGATGAC 
TGCATTGTGC TGAGAAGCAA CATTGGAACT GTGTATGAAC GTTGGTGGTA TGAGAAACTC 
ATCAACATGA CTTACTGTCC CAAAACAAAA GTTCTGTGTC TCTGGCGGAG GAATGGTCAG 
GAAACACAAC TGAACAAGTT CTACACAAAG AAGTGTCGGG AATTATACTA CTGCGTAAAA 
GACAGCATGG AGCGAGCAGC AGCAAGACAG CAAAGCATTA AGCCAGGCCC TGAGTTGGGT 
GGTGAGTTTC CTGTGCAGGA TATGAAAACT GGCGAAGGAG GTCTTCTGCA GGTGACACTG 
GAAGGAATTA ACCTGAAATT TATGCACAGT CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC TGTGCGAGGA GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ACTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCAATGG CTCACGAGAT CTGCTACTCC 
GTGCTGTGTC TCTTCTCTTA TGTGGCTGCC ATTCGTGGGA AAGAGGCAGA AAACAAAAGC 
AAACCACCTC GGCCAGTTTC AAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142660.1
CDS	233..5119
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIGPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287174.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287174.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG TAGGGATAAA GGATCTGTCT GGGACCAGTT AGAGGATGCC 
GCAATGGAAA CCTTCTCTAT GAGCAAAGAG CGCTCAACAC TGTGGGACCA GATGCAATTC 
TGGGAAGATG CCTTTTTGGA TGCTGTGATG TTAGAGAGAG AAGGAATGGG GATGGATCAG 
GGACCCCAGG AGATGATTGA CAGGTATCTT TCCCTGGGAG AACATGATCG AAAACGTTTG 
GAGGATGATG AGGACCGCTT ATTGGCTACG CTGCTACATA ATATGATTGC TTATATGCTT 
ATGATAAAGG TGAATAAGAA TGATATTAGG AAAAAGGTGC GTCGTCTGAT GGGAAAATCG 
CATATTGGAT TGTTGCACAG CCAGCAGTTA AATGACATCC TAGACAAGCT TGCTAACCTG 
AATGGACGGG AACTTCCTGT GAGACCCAGC GGCAGCCGCC ACATCAAGAA GCAGACTTTT 
GTAGTCCATG CTGGGACAGA CACTACAGGA GATATATTTT TCATGGAGGT TTGTGATGAC 
TGCATTGTGC TGAGAAGCAA CATTGGAACT GTGTATGAAC GTTGGTGGTA TGAGAAACTC 
ATCAACATGA CTTACTGTCC CAAAACAAAA GTTCTGTGTC TCTGGCGGAG GAATGGTCAG 
GAAACACAAC TGAACAAGTT CTACACAAAG AAGTGTCGGG AATTATACTA CTGCGTAAAA 
GACAGCATGG AGCGAGCAGC AGCAAGACAG CAAAGCATTA AGCCAGGCCC TGAGTTGGGT 
GGTGAGTTTC CTGTGCAGGA TATGAAAACT GGCGAAGGAG GTCTTCTGCA GGTGACACTG 
GAAGGAATTA ACCTGAAATT TATGCACAGT CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC TGTGCGAGGA GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ACTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCAATGG CTCACGAGAT CTGCTACTCC 
GTGCTGTGTC TCTTCTCTTA TGTGGCTGCC ATTCGTGGGA AAGAGGCAGA AAACAAAAGC 
AAACCACCTC GGCCAGTTTC AAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287174.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142660.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X20, mRNA.

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CloneID	OGa36685
Clone ID Related Accession (Same CDS sequence)	XM_015287174.1 , XM_015287174.2
Accession Version	XM_015287174.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287174.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG TAGGGATAAA GGATCTGTCT GGGACCAGTT AGAGGATGCC 
GCAATGGAAA CCTTCTCTAT GAGCAAAGAG CGCTCAACAC TGTGGGACCA GATGCAATTC 
TGGGAAGATG CCTTTTTGGA TGCTGTGATG TTAGAGAGAG AAGGAATGGG GATGGATCAG 
GGACCCCAGG AGATGATTGA CAGGTATCTT TCCCTGGGAG AACATGATCG AAAACGTTTG 
GAGGATGATG AGGACCGCTT ATTGGCTACG CTGCTACATA ATATGATTGC TTATATGCTT 
ATGATAAAGG TGAATAAGAA TGATATTAGG AAAAAGGTGC GTCGTCTGAT GGGAAAATCG 
CATATTGGAT TGTTGCACAG CCAGCAGTTA AATGACATCC TAGACAAGCT TGCTAACCTG 
AATGGACGGG AACTTCCTGT GAGACCCAGC GGCAGCCGCC ACATCAAGAA GCAGACTTTT 
GTAGTCCATG CTGGGACAGA CACTACAGGA GATATATTTT TCATGGAGGT TTGTGATGAC 
TGCATTGTGC TGAGAAGCAA CATTGGAACT GTGTATGAAC GTTGGTGGTA TGAGAAACTC 
ATCAACATGA CTTACTGTCC CAAAACAAAA GTTCTGTGTC TCTGGCGGAG GAATGGTCAG 
GAAACACAAC TGAACAAGTT CTACACAAAG AAGTGTCGGG AATTATACTA CTGCGTAAAA 
GACAGCATGG AGCGAGCAGC AGCAAGACAG CAAAGCATTA AGCCAGGCCC TGAGTTGGGT 
GGTGAGTTTC CTGTGCAGGA TATGAAAACT GGCGAAGGAG GTCTTCTGCA GGTGACACTG 
GAAGGAATTA ACCTGAAATT TATGCACAGT CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC TGTGCGAGGA GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ACTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCAATGG CTCACGAGAT CTGCTACTCC 
GTGCTGTGTC TCTTCTCTTA TGTGGCTGCC ATTCGTGGGA AAGAGGCAGA AAACAAAAGC 
AAACCACCTC GGCCAGTTTC AAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142660.1
CDS	277..5163
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIGPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287174.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287174.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG TAGGGATAAA GGATCTGTCT GGGACCAGTT AGAGGATGCC 
GCAATGGAAA CCTTCTCTAT GAGCAAAGAG CGCTCAACAC TGTGGGACCA GATGCAATTC 
TGGGAAGATG CCTTTTTGGA TGCTGTGATG TTAGAGAGAG AAGGAATGGG GATGGATCAG 
GGACCCCAGG AGATGATTGA CAGGTATCTT TCCCTGGGAG AACATGATCG AAAACGTTTG 
GAGGATGATG AGGACCGCTT ATTGGCTACG CTGCTACATA ATATGATTGC TTATATGCTT 
ATGATAAAGG TGAATAAGAA TGATATTAGG AAAAAGGTGC GTCGTCTGAT GGGAAAATCG 
CATATTGGAT TGTTGCACAG CCAGCAGTTA AATGACATCC TAGACAAGCT TGCTAACCTG 
AATGGACGGG AACTTCCTGT GAGACCCAGC GGCAGCCGCC ACATCAAGAA GCAGACTTTT 
GTAGTCCATG CTGGGACAGA CACTACAGGA GATATATTTT TCATGGAGGT TTGTGATGAC 
TGCATTGTGC TGAGAAGCAA CATTGGAACT GTGTATGAAC GTTGGTGGTA TGAGAAACTC 
ATCAACATGA CTTACTGTCC CAAAACAAAA GTTCTGTGTC TCTGGCGGAG GAATGGTCAG 
GAAACACAAC TGAACAAGTT CTACACAAAG AAGTGTCGGG AATTATACTA CTGCGTAAAA 
GACAGCATGG AGCGAGCAGC AGCAAGACAG CAAAGCATTA AGCCAGGCCC TGAGTTGGGT 
GGTGAGTTTC CTGTGCAGGA TATGAAAACT GGCGAAGGAG GTCTTCTGCA GGTGACACTG 
GAAGGAATTA ACCTGAAATT TATGCACAGT CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC TGTGCGAGGA GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ACTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCAATGG CTCACGAGAT CTGCTACTCC 
GTGCTGTGTC TCTTCTCTTA TGTGGCTGCC ATTCGTGGGA AAGAGGCAGA AAACAAAAGC 
AAACCACCTC GGCCAGTTTC AAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287175.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142661.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X21, mRNA.

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CloneID	OGa36686
Clone ID Related Accession (Same CDS sequence)	XM_015287175.1 , XM_015287175.2
Accession Version	XM_015287175.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG GAGCGGAAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACTGT GCGAGGAGTC TTTGTCCTGG AGGAATTTGT TCCTGAAACT 
AAAGAAGTGG TGAGCCACAA GTACAAGACG CCAATGGCTC ACGAGATCTG CTACTCCGTG 
CTGTGTCTCT TCTCTTATGT GGCTGCCATT CGTGGGAAAG AGGCAGAAAA CAAAAGCAAA 
CCACCTCGGC CAGTTTCAAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142661.1
CDS	233..5116
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIGPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287175.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287175.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG GAGCGGAAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACTGT GCGAGGAGTC TTTGTCCTGG AGGAATTTGT TCCTGAAACT 
AAAGAAGTGG TGAGCCACAA GTACAAGACG CCAATGGCTC ACGAGATCTG CTACTCCGTG 
CTGTGTCTCT TCTCTTATGT GGCTGCCATT CGTGGGAAAG AGGCAGAAAA CAAAAGCAAA 
CCACCTCGGC CAGTTTCAAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287175.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142661.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X21, mRNA.

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CloneID	OGa36686
Clone ID Related Accession (Same CDS sequence)	XM_015287175.1 , XM_015287175.2
Accession Version	XM_015287175.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287175.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG GAGCGGAAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACTGT GCGAGGAGTC TTTGTCCTGG AGGAATTTGT TCCTGAAACT 
AAAGAAGTGG TGAGCCACAA GTACAAGACG CCAATGGCTC ACGAGATCTG CTACTCCGTG 
CTGTGTCTCT TCTCTTATGT GGCTGCCATT CGTGGGAAAG AGGCAGAAAA CAAAAGCAAA 
CCACCTCGGC CAGTTTCAAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142661.1
CDS	277..5160
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIGPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287175.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287175.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG GAGCGGAAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACTGT GCGAGGAGTC TTTGTCCTGG AGGAATTTGT TCCTGAAACT 
AAAGAAGTGG TGAGCCACAA GTACAAGACG CCAATGGCTC ACGAGATCTG CTACTCCGTG 
CTGTGTCTCT TCTCTTATGT GGCTGCCATT CGTGGGAAAG AGGCAGAAAA CAAAAGCAAA 
CCACCTCGGC CAGTTTCAAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287178.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142664.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OGa36687
Clone ID Related Accession (Same CDS sequence)	XM_015287178.1 , XM_015287178.2
Accession Version	XM_015287178.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGTGT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142664.1
CDS	233..5107
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKDYFNLEVGRDVNEVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVC

Target ORF information:

RefSeq Version	XM_015287178.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287178.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGTGT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287178.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142664.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OGa36687
Clone ID Related Accession (Same CDS sequence)	XM_015287178.1 , XM_015287178.2
Accession Version	XM_015287178.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287178.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGTGT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142664.1
CDS	277..5151
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKDYFNLEVGRDVNEVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVC

Target ORF information:

RefSeq Version	XM_015287178.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287178.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GATTACTTTA ATCTGGAAGT GGGAAGGGAT GTGAATGAAG TATTTGGGTT AAATACTATA 
ATGGAGATTA TTACTGAAGC TGGCCCAGTA AGCAATGAAG GAAACAGGAG AGCCCTGGTG 
GATCAAAAGT CTTCAGTCAT TAAGCATAGC CCAACAGTGA AGAGGGAATC TCCATCACCT 
CAGGGACGAA GTAGTAACTC CAGTGAGAAC CAGCAATTCC TGAAGGAGGT GGTACACAAT 
GTTCTCGATG GGCAAGGTGT TGGTTGGCTG AACATGAAGA GAGTCCGACG TCTGCTGGAG 
AGTGAGCAGC TCCGTGTCTT TGTACTAAGC AAGCTAAATC GCACGATCCA GTCGGAAGAA 
GATGCTCAGC AGGATATCAT ACAGGATGTG GAGATCAGCC GCAAAGTTTA TAAAGGCATG 
CTGGACTTGC TAAAGTGTAC TGTGCTGAGC CTGGAGCAGT CATACGCAAA TGCTGGCCTG 
GGAGGCATGG CCAGTGTTTT TGGCCTTCTA GAAATAGCAC ATACTCACTA TTACAACAAA 
GAACCAGAAA AGAGAAAACG AAGCCCAACA GATGGATCTG TCACTCCGGT TGGCAAAGAT 
CCTGCATCAT CTCCAAGAGT GGAGCCGAAA CCTGCCATGC AGCTGCCAGT ACCACAGCTG 
ATGCCGAAGG CACCAAGCCC TGCAGGGAAA GGGCTAAGGG AGTTTGATAC AAGGAGTCTA 
AAGGAAGAAA ATTTTATTGC TTCCATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAATCTT TGGAAAAAAC GAGGCCAGAA GGTGTGAAAC ATCTTGATTT GGGAGAAACA 
GATGAGAAAA AATCCCAAAT CAGTGCAGAC AGTGGCCTCA GTTTGGCCTC AGGTTCTCAG 
AAGAGTGATT TTGACTCTGT TCCCAGTGGA GGACCGTCAG TTATGGTCCG AAGTACCAGC 
CAGGATTCTG AAGTTAGCAC AGTGGTTAGT AACAGCTCTG GGGAGACACT AGGAGCAGAC 
AGTGACTTGA GTAGCAATGC TGGTGATGGC CCAAGTGTGG AAAATGGAGG CAATCTGGCA 
GGATCCAGGG GCACCGTGTC AGACAGTGAA ATTGAGACAA ACTCTGCTAC TAGCTCTATT 
TTCGCAAAGT CTCATAACTT GAAGCAGAAT GTAAAGGAGA ACAAAGGCAG TACTGCAGGG 
AAAGGTCCAG AGGATGGGAA CCAGCGTGTC TATCTCTATG AAGGACTTTT GGGTAGGGAT 
AAAGGATCTG TCTGGGACCA GTTAGAGGAT GCCGCAATGG AAACCTTCTC TATGAGCAAA 
GAGCGCTCAA CACTGTGGGA CCAGATGCAA TTCTGGGAAG ATGCCTTTTT GGATGCTGTG 
ATGTTAGAGA GAGAAGGAAT GGGGATGGAT CAGGGACCCC AGGAGATGAT TGACAGGTAT 
CTTTCCCTGG GAGAACATGA TCGAAAACGT TTGGAGGATG ATGAGGACCG CTTATTGGCT 
ACGCTGCTAC ATAATATGAT TGCTTATATG CTTATGATAA AGGTGAATAA GAATGATATT 
AGGAAAAAGG TGCGTCGTCT GATGGGAAAA TCGCATATTG GATTGTTGCA CAGCCAGCAG 
TTAAATGACA TCCTAGACAA GCTTGCTAAC CTGAATGGAC GGGAACTTCC TGTGAGACCC 
AGCGGCAGCC GCCACATCAA GAAGCAGACT TTTGTAGTCC ATGCTGGGAC AGACACTACA 
GGAGATATAT TTTTCATGGA GGTTTGTGAT GACTGCATTG TGCTGAGAAG CAACATTGGA 
ACTGTGTATG AACGTTGGTG GTATGAGAAA CTCATCAACA TGACTTACTG TCCCAAAACA 
AAAGTTCTGT GTCTCTGGCG GAGGAATGGT CAGGAAACAC AACTGAACAA GTTCTACACA 
AAGAAGTGTC GGGAATTATA CTACTGCGTA AAAGACAGCA TGGAGCGAGC AGCAGCAAGA 
CAGCAAAGCA TTAAGCCAGG CCCTGAGTTG GGTGGTGAGT TTCCTGTGCA GGATATGAAA 
ACTGGCGAAG GAGGTCTTCT GCAGGTGACA CTGGAAGGAA TTAACCTGAA ATTTATGCAC 
AGTCAGGTGT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287159.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142645.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X6, mRNA.

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CloneID	OGa09157
Clone ID Related Accession (Same CDS sequence)	XM_015287159.2 , XM_015287159.1
Accession Version	XM_015287159.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287159.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACTGTGCG AGGAGTCTTT GTCCTGGAGG AATTTGTTCC TGAAACTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCTCACG AGATCTGCTA CTCCGTGCTG 
TGTCTCTTCT CTTATGTGGC TGCCATTCGT GGGAAAGAGG CAGAAAACAA AAGCAAACCA 
CCTCGGCCAG TTTCAAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142645.1
CDS	277..5337
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287159.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287159.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACTGTGCG AGGAGTCTTT GTCCTGGAGG AATTTGTTCC TGAAACTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCTCACG AGATCTGCTA CTCCGTGCTG 
TGTCTCTTCT CTTATGTGGC TGCCATTCGT GGGAAAGAGG CAGAAAACAA AAGCAAACCA 
CCTCGGCCAG TTTCAAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287159.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142645.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X6, mRNA.

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CloneID	OGa09157
Clone ID Related Accession (Same CDS sequence)	XM_015287159.2 , XM_015287159.1
Accession Version	XM_015287159.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACTGTGCG AGGAGTCTTT GTCCTGGAGG AATTTGTTCC TGAAACTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCTCACG AGATCTGCTA CTCCGTGCTG 
TGTCTCTTCT CTTATGTGGC TGCCATTCGT GGGAAAGAGG CAGAAAACAA AAGCAAACCA 
CCTCGGCCAG TTTCAAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142645.1
CDS	233..5293
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287159.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287159.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACTGTGCG AGGAGTCTTT GTCCTGGAGG AATTTGTTCC TGAAACTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCTCACG AGATCTGCTA CTCCGTGCTG 
TGTCTCTTCT CTTATGTGGC TGCCATTCGT GGGAAAGAGG CAGAAAACAA AAGCAAACCA 
CCTCGGCCAG TTTCAAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287168.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142654.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X14, mRNA.

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CloneID	OGa09162
Clone ID Related Accession (Same CDS sequence)	XM_015287168.1 , XM_015287168.2
Accession Version	XM_015287168.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACTGTGCG AGGAGTCTTT GTCCTGGAGG AATTTGTTCC TGAAACTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCTCACG AGATCTGCTA CTCCGTGCTG 
TGTCTCTTCT CTTATGTGGC TGCCATTCGT GGGAAAGAGG CAGAAAACAA AAGCAAACCA 
CCTCGGCCAG TTTCAAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142654.1
CDS	233..5233
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287168.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287168.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACTGTGCG AGGAGTCTTT GTCCTGGAGG AATTTGTTCC TGAAACTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCTCACG AGATCTGCTA CTCCGTGCTG 
TGTCTCTTCT CTTATGTGGC TGCCATTCGT GGGAAAGAGG CAGAAAACAA AAGCAAACCA 
CCTCGGCCAG TTTCAAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287168.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142654.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X14, mRNA.

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CloneID	OGa09162
Clone ID Related Accession (Same CDS sequence)	XM_015287168.1 , XM_015287168.2
Accession Version	XM_015287168.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287168.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACTGTGCG AGGAGTCTTT GTCCTGGAGG AATTTGTTCC TGAAACTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCTCACG AGATCTGCTA CTCCGTGCTG 
TGTCTCTTCT CTTATGTGGC TGCCATTCGT GGGAAAGAGG CAGAAAACAA AAGCAAACCA 
CCTCGGCCAG TTTCAAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142654.1
CDS	277..5277
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287168.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287168.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACTGTGCG AGGAGTCTTT GTCCTGGAGG AATTTGTTCC TGAAACTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCTCACG AGATCTGCTA CTCCGTGCTG 
TGTCTCTTCT CTTATGTGGC TGCCATTCGT GGGAAAGAGG CAGAAAACAA AAGCAAACCA 
CCTCGGCCAG TTTCAAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287167.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142653.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OGa09163
Clone ID Related Accession (Same CDS sequence)	XM_015287167.2 , XM_015287167.1
Accession Version	XM_015287167.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287167.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACTGTGCG AGGAGTCTTT GTCCTGGAGG AATTTGTTCC TGAAACTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCTCACG AGATCTGCTA CTCCGTGCTG 
TGTCTCTTCT CTTATGTGGC TGCCATTCGT GGGAAAGAGG CAGAAAACAA AAGCAAACCA 
CCTCGGCCAG TTTCAAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142653.1
CDS	277..5277
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287167.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287167.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACTGTGCG AGGAGTCTTT GTCCTGGAGG AATTTGTTCC TGAAACTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCTCACG AGATCTGCTA CTCCGTGCTG 
TGTCTCTTCT CTTATGTGGC TGCCATTCGT GGGAAAGAGG CAGAAAACAA AAGCAAACCA 
CCTCGGCCAG TTTCAAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287167.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142653.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OGa09163
Clone ID Related Accession (Same CDS sequence)	XM_015287167.2 , XM_015287167.1
Accession Version	XM_015287167.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACTGTGCG AGGAGTCTTT GTCCTGGAGG AATTTGTTCC TGAAACTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCTCACG AGATCTGCTA CTCCGTGCTG 
TGTCTCTTCT CTTATGTGGC TGCCATTCGT GGGAAAGAGG CAGAAAACAA AAGCAAACCA 
CCTCGGCCAG TTTCAAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142653.1
CDS	233..5233
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287167.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287167.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACTGTGCG AGGAGTCTTT GTCCTGGAGG AATTTGTTCC TGAAACTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCTCACG AGATCTGCTA CTCCGTGCTG 
TGTCTCTTCT CTTATGTGGC TGCCATTCGT GGGAAAGAGG CAGAAAACAA AAGCAAACCA 
CCTCGGCCAG TTTCAAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287169.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142655.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X15, mRNA.

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CloneID	OGa09165
Clone ID Related Accession (Same CDS sequence)	XM_015287169.2 , XM_015287169.1
Accession Version	XM_015287169.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287169.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC 
AATACTGTGC GAGGAGTCTT TGTCCTGGAG GAATTTGTTC CTGAAACTAA AGAAGTGGTG 
AGCCACAAGT ACAAGACGCC AATGGCTCAC GAGATCTGCT ACTCCGTGCT GTGTCTCTTC 
TCTTATGTGG CTGCCATTCG TGGGAAAGAG GCAGAAAACA AAAGCAAACC ACCTCGGCCA 
GTTTCAAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142655.1
CDS	277..5268
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287169.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287169.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC 
AATACTGTGC GAGGAGTCTT TGTCCTGGAG GAATTTGTTC CTGAAACTAA AGAAGTGGTG 
AGCCACAAGT ACAAGACGCC AATGGCTCAC GAGATCTGCT ACTCCGTGCT GTGTCTCTTC 
TCTTATGTGG CTGCCATTCG TGGGAAAGAG GCAGAAAACA AAAGCAAACC ACCTCGGCCA 
GTTTCAAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287169.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142655.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X15, mRNA.

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CloneID	OGa09165
Clone ID Related Accession (Same CDS sequence)	XM_015287169.2 , XM_015287169.1
Accession Version	XM_015287169.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC 
AATACTGTGC GAGGAGTCTT TGTCCTGGAG GAATTTGTTC CTGAAACTAA AGAAGTGGTG 
AGCCACAAGT ACAAGACGCC AATGGCTCAC GAGATCTGCT ACTCCGTGCT GTGTCTCTTC 
TCTTATGTGG CTGCCATTCG TGGGAAAGAG GCAGAAAACA AAAGCAAACC ACCTCGGCCA 
GTTTCAAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142655.1
CDS	233..5224
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287169.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287169.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC 
AATACTGTGC GAGGAGTCTT TGTCCTGGAG GAATTTGTTC CTGAAACTAA AGAAGTGGTG 
AGCCACAAGT ACAAGACGCC AATGGCTCAC GAGATCTGCT ACTCCGTGCT GTGTCTCTTC 
TCTTATGTGG CTGCCATTCG TGGGAAAGAG GCAGAAAACA AAAGCAAACC ACCTCGGCCA 
GTTTCAAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287171.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142657.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X17, mRNA.

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CloneID	OGa09166
Clone ID Related Accession (Same CDS sequence)	XM_015287171.1 , XM_015287171.2
Accession Version	XM_015287171.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACTGTGCG AGGAGTCTTT GTCCTGGAGG AATTTGTTCC TGAAACTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCTCACG AGATCTGCTA CTCCGTGCTG 
TGTCTCTTCT CTTATGTGGC TGCCATTCGT GGGAAAGAGG CAGAAAACAA AAGCAAACCA 
CCTCGGCCAG TTTCAAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142657.1
CDS	233..5173
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287171.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287171.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACTGTGCG AGGAGTCTTT GTCCTGGAGG AATTTGTTCC TGAAACTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCTCACG AGATCTGCTA CTCCGTGCTG 
TGTCTCTTCT CTTATGTGGC TGCCATTCGT GGGAAAGAGG CAGAAAACAA AAGCAAACCA 
CCTCGGCCAG TTTCAAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287171.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142657.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X17, mRNA.

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CloneID	OGa09166
Clone ID Related Accession (Same CDS sequence)	XM_015287171.1 , XM_015287171.2
Accession Version	XM_015287171.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287171.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACTGTGCG AGGAGTCTTT GTCCTGGAGG AATTTGTTCC TGAAACTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCTCACG AGATCTGCTA CTCCGTGCTG 
TGTCTCTTCT CTTATGTGGC TGCCATTCGT GGGAAAGAGG CAGAAAACAA AAGCAAACCA 
CCTCGGCCAG TTTCAAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142657.1
CDS	277..5217
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287171.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287171.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACTGTGCG AGGAGTCTTT GTCCTGGAGG AATTTGTTCC TGAAACTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCTCACG AGATCTGCTA CTCCGTGCTG 
TGTCTCTTCT CTTATGTGGC TGCCATTCGT GGGAAAGAGG CAGAAAACAA AAGCAAACCA 
CCTCGGCCAG TTTCAAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287172.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142658.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OGa09167
Clone ID Related Accession (Same CDS sequence)	XM_015287172.1 , XM_015287172.2
Accession Version	XM_015287172.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACTG TGCGAGGAGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCAATGGCT CACGAGATCT GCTACTCCGT GCTGTGTCTC 
TTCTCTTATG TGGCTGCCAT TCGTGGGAAA GAGGCAGAAA ACAAAAGCAA ACCACCTCGG 
CCAGTTTCAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142658.1
CDS	233..5167
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIGPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287172.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287172.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACTG TGCGAGGAGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCAATGGCT CACGAGATCT GCTACTCCGT GCTGTGTCTC 
TTCTCTTATG TGGCTGCCAT TCGTGGGAAA GAGGCAGAAA ACAAAAGCAA ACCACCTCGG 
CCAGTTTCAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287172.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142658.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OGa09167
Clone ID Related Accession (Same CDS sequence)	XM_015287172.1 , XM_015287172.2
Accession Version	XM_015287172.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287172.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACTG TGCGAGGAGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCAATGGCT CACGAGATCT GCTACTCCGT GCTGTGTCTC 
TTCTCTTATG TGGCTGCCAT TCGTGGGAAA GAGGCAGAAA ACAAAAGCAA ACCACCTCGG 
CCAGTTTCAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142658.1
CDS	277..5211
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIGPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287172.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287172.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACTG TGCGAGGAGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCAATGGCT CACGAGATCT GCTACTCCGT GCTGTGTCTC 
TTCTCTTATG TGGCTGCCAT TCGTGGGAAA GAGGCAGAAA ACAAAAGCAA ACCACCTCGG 
CCAGTTTCAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287177.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142663.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X23, mRNA.

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CloneID	OGa09168
Clone ID Related Accession (Same CDS sequence)	XM_015287177.2 , XM_015287177.1
Accession Version	XM_015287177.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287177.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACTG TGCGAGGAGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCAATGGCT CACGAGATCT GCTACTCCGT GCTGTGTCTC 
TTCTCTTATG TGGCTGCCAT TCGTGGGAAA GAGGCAGAAA ACAAAAGCAA ACCACCTCGG 
CCAGTTTCAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142663.1
CDS	277..5151
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIGPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287177.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287177.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACTG TGCGAGGAGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCAATGGCT CACGAGATCT GCTACTCCGT GCTGTGTCTC 
TTCTCTTATG TGGCTGCCAT TCGTGGGAAA GAGGCAGAAA ACAAAAGCAA ACCACCTCGG 
CCAGTTTCAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287177.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142663.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X23, mRNA.

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CloneID	OGa09168
Clone ID Related Accession (Same CDS sequence)	XM_015287177.2 , XM_015287177.1
Accession Version	XM_015287177.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACTG TGCGAGGAGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCAATGGCT CACGAGATCT GCTACTCCGT GCTGTGTCTC 
TTCTCTTATG TGGCTGCCAT TCGTGGGAAA GAGGCAGAAA ACAAAAGCAA ACCACCTCGG 
CCAGTTTCAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142663.1
CDS	233..5107
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIGPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287177.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287177.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACTG TGCGAGGAGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCAATGGCT CACGAGATCT GCTACTCCGT GCTGTGTCTC 
TTCTCTTATG TGGCTGCCAT TCGTGGGAAA GAGGCAGAAA ACAAAAGCAA ACCACCTCGG 
CCAGTTTCAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287176.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142662.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X22, mRNA.

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CloneID	OGa09169
Clone ID Related Accession (Same CDS sequence)	XM_015287176.1 , XM_015287176.2
Accession Version	XM_015287176.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACTG TGCGAGGAGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCAATGGCT CACGAGATCT GCTACTCCGT GCTGTGTCTC 
TTCTCTTATG TGGCTGCCAT TCGTGGGAAA GAGGCAGAAA ACAAAAGCAA ACCACCTCGG 
CCAGTTTCAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142662.1
CDS	233..5107
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIGPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287176.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287176.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACTG TGCGAGGAGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCAATGGCT CACGAGATCT GCTACTCCGT GCTGTGTCTC 
TTCTCTTATG TGGCTGCCAT TCGTGGGAAA GAGGCAGAAA ACAAAAGCAA ACCACCTCGG 
CCAGTTTCAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287176.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142662.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X22, mRNA.

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CloneID	OGa09169
Clone ID Related Accession (Same CDS sequence)	XM_015287176.1 , XM_015287176.2
Accession Version	XM_015287176.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287176.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACTG TGCGAGGAGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCAATGGCT CACGAGATCT GCTACTCCGT GCTGTGTCTC 
TTCTCTTATG TGGCTGCCAT TCGTGGGAAA GAGGCAGAAA ACAAAAGCAA ACCACCTCGG 
CCAGTTTCAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142662.1
CDS	277..5151
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIGPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287176.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287176.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACTG TGCGAGGAGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCAATGGCT CACGAGATCT GCTACTCCGT GCTGTGTCTC 
TTCTCTTATG TGGCTGCCAT TCGTGGGAAA GAGGCAGAAA ACAAAAGCAA ACCACCTCGG 
CCAGTTTCAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287179.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142665.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X25, mRNA.

Price & Availability↑

CloneID	OGa09171
Clone ID Related Accession (Same CDS sequence)	XM_015287179.1 , XM_015287179.2
Accession Version	XM_015287179.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG GAGCGGAAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACTGT GCGAGGAGTC TTTGTCCTGG AGGAATTTGT TCCTGAAACT 
AAAGAAGTGG TGAGCCACAA GTACAAGACG CCAATGGCTC ACGAGATCTG CTACTCCGTG 
CTGTGTCTCT TCTCTTATGT GGCTGCCATT CGTGGGAAAG AGGCAGAAAA CAAAAGCAAA 
CCACCTCGGC CAGTTTCAAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142665.1
CDS	233..5056
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIGPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287179.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287179.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG GAGCGGAAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACTGT GCGAGGAGTC TTTGTCCTGG AGGAATTTGT TCCTGAAACT 
AAAGAAGTGG TGAGCCACAA GTACAAGACG CCAATGGCTC ACGAGATCTG CTACTCCGTG 
CTGTGTCTCT TCTCTTATGT GGCTGCCATT CGTGGGAAAG AGGCAGAAAA CAAAAGCAAA 
CCACCTCGGC CAGTTTCAAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287179.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142665.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X25, mRNA.

Price & Availability↑

CloneID	OGa09171
Clone ID Related Accession (Same CDS sequence)	XM_015287179.1 , XM_015287179.2
Accession Version	XM_015287179.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287179.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG GAGCGGAAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACTGT GCGAGGAGTC TTTGTCCTGG AGGAATTTGT TCCTGAAACT 
AAAGAAGTGG TGAGCCACAA GTACAAGACG CCAATGGCTC ACGAGATCTG CTACTCCGTG 
CTGTGTCTCT TCTCTTATGT GGCTGCCATT CGTGGGAAAG AGGCAGAAAA CAAAAGCAAA 
CCACCTCGGC CAGTTTCAAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142665.1
CDS	277..5100
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIGPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287179.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287179.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG GAGCGGAAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACTGT GCGAGGAGTC TTTGTCCTGG AGGAATTTGT TCCTGAAACT 
AAAGAAGTGG TGAGCCACAA GTACAAGACG CCAATGGCTC ACGAGATCTG CTACTCCGTG 
CTGTGTCTCT TCTCTTATGT GGCTGCCATT CGTGGGAAAG AGGCAGAAAA CAAAAGCAAA 
CCACCTCGGC CAGTTTCAAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287180.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142666.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X26, mRNA.

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CloneID	OGa09172
Clone ID Related Accession (Same CDS sequence)	XM_015287180.2 , XM_015287180.1
Accession Version	XM_015287180.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287180.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACTG TGCGAGGAGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCAATGGCT CACGAGATCT GCTACTCCGT GCTGTGTCTC 
TTCTCTTATG TGGCTGCCAT TCGTGGGAAA GAGGCAGAAA ACAAAAGCAA ACCACCTCGG 
CCAGTTTCAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142666.1
CDS	277..5091
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIGPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287180.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287180.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACTG TGCGAGGAGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCAATGGCT CACGAGATCT GCTACTCCGT GCTGTGTCTC 
TTCTCTTATG TGGCTGCCAT TCGTGGGAAA GAGGCAGAAA ACAAAAGCAA ACCACCTCGG 
CCAGTTTCAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287180.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142666.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X26, mRNA.

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CloneID	OGa09172
Clone ID Related Accession (Same CDS sequence)	XM_015287180.2 , XM_015287180.1
Accession Version	XM_015287180.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACTG TGCGAGGAGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCAATGGCT CACGAGATCT GCTACTCCGT GCTGTGTCTC 
TTCTCTTATG TGGCTGCCAT TCGTGGGAAA GAGGCAGAAA ACAAAAGCAA ACCACCTCGG 
CCAGTTTCAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142666.1
CDS	233..5047
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIGPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIELNHIKKCNTVRGVFVLEEFVPETKEVVSHKYKTPMAHEICYSVLCLFSYVAAIRGKEAENKSKPPRPVSS

Target ORF information:

RefSeq Version	XM_015287180.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287180.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACTG TGCGAGGAGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAC TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCAATGGCT CACGAGATCT GCTACTCCGT GCTGTGTCTC 
TTCTCTTATG TGGCTGCCAT TCGTGGGAAA GAGGCAGAAA ACAAAAGCAA ACCACCTCGG 
CCAGTTTCAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287181.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142667.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X27, mRNA.

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CloneID	OGa09174
Clone ID Related Accession (Same CDS sequence)	XM_015287181.2 , XM_015287181.1
Accession Version	XM_015287181.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287181.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGTTC CTGAAACTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142667.1
CDS	277..5067
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_015287181.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287181.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGTTC CTGAAACTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287181.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142667.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OGa09174
Clone ID Related Accession (Same CDS sequence)	XM_015287181.2 , XM_015287181.1
Accession Version	XM_015287181.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGTTC CTGAAACTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142667.1
CDS	233..5023
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKVFGLNTIMEIITEAGPVSNEGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_015287181.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287181.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GTATTTGGGT TAAATACTAT AATGGAGATT ATTACTGAAG CTGGCCCAGT AAGCAATGAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGTTC CTGAAACTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287182.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142668.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X28, mRNA.

Price & Availability↑

CloneID	OGa09175
Clone ID Related Accession (Same CDS sequence)	XM_015287182.2 , XM_015287182.1
Accession Version	XM_015287182.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287182.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGTTC CTGAAACTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142668.1
CDS	277..5007
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_015287182.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287182.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGTTC CTGAAACTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287182.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142668.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X28, mRNA.

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CloneID	OGa09175
Clone ID Related Accession (Same CDS sequence)	XM_015287182.2 , XM_015287182.1
Accession Version	XM_015287182.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGTTC CTGAAACTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142668.1
CDS	233..4963
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIELWNKHQEVKKQKSLEKTRPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGRDKGSVWDQLEDAAMETFSMSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_015287182.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287182.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAATCT TTGGAAAAAA CGAGGCCAGA AGGTGTGAAA 
CATCTTGATT TGGGAGAAAC AGATGAGAAA AAATCCCAAA TCAGTGCAGA CAGTGGCCTC 
AGTTTGGCCT CAGGTTCTCA GAAGAGTGAT TTTGACTCTG TTCCCAGTGG AGGACCGTCA 
GTTATGGTCC GAAGTACCAG CCAGGATTCT GAAGTTAGCA CAGTGGTTAG TAACAGCTCT 
GGGGAGACAC TAGGAGCAGA CAGTGACTTG AGTAGCAATG CTGGTGATGG CCCAAGTGTG 
GAAAATGGAG GCAATCTGGC AGGATCCAGG GGCACCGTGT CAGACAGTGA AATTGAGACA 
AACTCTGCTA CTAGCTCTAT TTTCGCAAAG TCTCATAACT TGAAGCAGAA TGTAAAGGAG 
AACAAAGGCA GTACTGCAGG GAAAGGTCCA GAGGATGGGA ACCAGCGTGT CTATCTCTAT 
GAAGGACTTT TGGGTAGGGA TAAAGGATCT GTCTGGGACC AGTTAGAGGA TGCCGCAATG 
GAAACCTTCT CTATGAGCAA AGAGCGCTCA ACACTGTGGG ACCAGATGCA ATTCTGGGAA 
GATGCCTTTT TGGATGCTGT GATGTTAGAG AGAGAAGGAA TGGGGATGGA TCAGGGACCC 
CAGGAGATGA TTGACAGGTA TCTTTCCCTG GGAGAACATG ATCGAAAACG TTTGGAGGAT 
GATGAGGACC GCTTATTGGC TACGCTGCTA CATAATATGA TTGCTTATAT GCTTATGATA 
AAGGTGAATA AGAATGATAT TAGGAAAAAG GTGCGTCGTC TGATGGGAAA ATCGCATATT 
GGATTGTTGC ACAGCCAGCA GTTAAATGAC ATCCTAGACA AGCTTGCTAA CCTGAATGGA 
CGGGAACTTC CTGTGAGACC CAGCGGCAGC CGCCACATCA AGAAGCAGAC TTTTGTAGTC 
CATGCTGGGA CAGACACTAC AGGAGATATA TTTTTCATGG AGGTTTGTGA TGACTGCATT 
GTGCTGAGAA GCAACATTGG AACTGTGTAT GAACGTTGGT GGTATGAGAA ACTCATCAAC 
ATGACTTACT GTCCCAAAAC AAAAGTTCTG TGTCTCTGGC GGAGGAATGG TCAGGAAACA 
CAACTGAACA AGTTCTACAC AAAGAAGTGT CGGGAATTAT ACTACTGCGT AAAAGACAGC 
ATGGAGCGAG CAGCAGCAAG ACAGCAAAGC ATTAAGCCAG GCCCTGAGTT GGGTGGTGAG 
TTTCCTGTGC AGGATATGAA AACTGGCGAA GGAGGTCTTC TGCAGGTGAC ACTGGAAGGA 
ATTAACCTGA AATTTATGCA CAGTCAGTTC CTGAAACTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287183.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142669.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X29, mRNA.

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CloneID	OGa09176
Clone ID Related Accession (Same CDS sequence)	XM_015287183.2 , XM_015287183.1
Accession Version	XM_015287183.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287183.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG TTCCTGAAAC TAAAGAAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142669.1
CDS	277..4950
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIGPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_015287183.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287183.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG TTCCTGAAAC TAAAGAAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287183.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142669.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X29, mRNA.

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CloneID	OGa09176
Clone ID Related Accession (Same CDS sequence)	XM_015287183.2 , XM_015287183.1
Accession Version	XM_015287183.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG TTCCTGAAAC TAAAGAAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142669.1
CDS	233..4906
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQCLVSMTAITQQQLLTPDNKALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIGPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_015287183.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287183.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGTG TCTGGTTAGC 
ATGACTGCAA TCACTCAGCA ACAGCTGCTC ACTCCTGACA ACAAGGCACT GGCCAGCATG 
AGTCTGAATA CTCAGCCCAT TCTTAATCTA GAGAAGTTCC ACGAGGGGCA GGAGGTGCCA 
CTGCTGCTGG GAAGACCACA GAATGATTTA CAGTCTACTC CCTCCACGGA ATTCAACCCT 
CTGATCTATG GAAATGATGT AGACTCTGTG GATGTGGCTA CCAGGGTTGC TATGGTGAGA 
TTCTTCAATT CACCAAATGT TTTGCAAGGA TTCCAGATGC ATACTCGCAC TCTTCGTCTT 
TTTCCACGAC CAGTAGTGGC CTTCCAGGCA AATTCCTTTC TTGCTTCTAG GCCAAAGCAA 
ACACCTTTTG CAGATAAGCT TTCCAGGACG CAAGCAGTAG AATATTTTGG AGAATGGTCA 
CTCAACCCTA CTAACTATGC TTTCCAAAGA ATTCATAACA ATATGTTTGA CCCAGCACTG 
ATTGGTGACA AACCGAAGTG GTATGCTCAC CAGCTGCAGC CCATCCACTA TCGTGTCTAT 
GACAGTAATT CACAACTAGC TGAAGCACTG AATGTTCCAG TAGAAAAAGA AACGGACTCT 
GACCCCACTG ATGACAGCAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG TTCCTGAAAC TAAAGAAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287184.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142670.1 Gallus gallus MAP kinase activating death domain (MADD), transcript variant X31, mRNA.

Price & Availability↑

CloneID	OGa09177
Clone ID Related Accession (Same CDS sequence)	XM_015287184.2 , XM_015287184.1
Accession Version	XM_015287184.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287184.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG TTCCTGAAAC TAAAGAAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142670.1
CDS	277..4890
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIGPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_015287184.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP kinase activating death domain (MADD), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287184.2

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG TTCCTGAAAC TAAAGAAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015287184.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142670.1 Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X30, mRNA.

Price & Availability↑

CloneID	OGa09177
Clone ID Related Accession (Same CDS sequence)	XM_015287184.2 , XM_015287184.1
Accession Version	XM_015287184.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	MAP kinase-activating death domain protein isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG TTCCTGAAAC TAAAGAAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142670.1
CDS	233..4846
Translation	MVQKKKSCPRLLDYLVIIGARQPSSDSVAQTPELLRRYPLEDHVDFPLPPDVVFFCQPEGCLSVRQKRMSFRDDTSFVFTLTDKDTGVIRYGICVNFYRSFQKRVPKEKGEGTGGHRAREGQKIAKSGDTSAPQEDVGTESSESGSSLQAPSTEPTPDVNRSPHSKRLAKGSHRSRNSTLTSLCILSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTTWRIFTGSLLVEEKSSTLLHDLREIEAWIYRLLRSPMPVAGQKRVDVEVLPHELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLIDLDTNKVIVPTNAESLPALPEPEASELKKHLKQALASMSLNTQPILNLEKFHEGQEVPLLLGRPQNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQANSFLASRPKQTPFADKLSRTQAVEYFGEWSLNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALNVPVEKETDSDPTDDSGSDSVDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEFDDFQEYSGELDEQAMDSENSQENNQPRSSSSTTASSSPSTVIHGVNHESADSAEMEEKLVAGFSNHLPSLPLQPSFPKMSFDRRESDSPAVSMSSSEGVVRKREYDNPYFEPQYGFPTEDEDDEQEESYTPRFNQNLNGSRSQKLLRPNSLKLANDSDADSDSRASSPNSTVSNNSSEGFGGIMSFASSLYRNHSTSFSLSNLALPTKVGRDKNTPFPSLKGNRRALVDQKSSVIKHSPTVKRESPSPQGRSSNSSENQQFLKEVVHNVLDGQGVGWLNMKRVRRLLESEQLRVFVLSKLNRTIQSEEDAQQDIIQDVEISRKVYKGMLDLLKCTVLSLEQSYANAGLGGMASVFGLLEIAHTHYYNKEPEKRKRSPTDGSVTPVGKDPASSPRVEPKPAMQLPVPQLMPKAPSPAGKGLREFDTRSLKEENFIASIGPEGVKHLDLGETDEKKSQISADSGLSLASGSQKSDFDSVPSGGPSVMVRSTSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPSVENGGNLAGSRGTVSDSEIETNSATSSIFAKSHNLKQNVKENKGSTAGKGPEDGNQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMIKVNKNDIRKKVRRLMGKSHIGLLHSQQLNDILDKLANLNGRELPVRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQFLKLKKW

Target ORF information:

RefSeq Version	XM_015287184.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MAP-kinase activating death domain (MADD), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287184.1

ORF Insert Sequence:

ATGGTGCAGA AAAAGAAAAG CTGCCCTCGG TTACTCGACT ACCTTGTTAT CATCGGAGCC 
AGGCAGCCAA GTAGTGATAG TGTTGCTCAG ACTCCTGAGC TGCTACGACG TTATCCTCTA 
GAAGACCACG TGGACTTTCC TCTACCACCT GACGTTGTGT TCTTCTGCCA GCCAGAAGGA 
TGCCTGAGTG TGCGGCAGAA ACGCATGAGC TTCCGTGATG ACACTTCTTT TGTCTTCACA 
CTCACAGACA AAGATACGGG TGTCATTCGC TATGGAATCT GTGTCAACTT CTACCGCTCC 
TTCCAGAAGC GAGTACCCAA GGAGAAGGGA GAAGGCACCG GAGGACATCG AGCTCGGGAG 
GGACAGAAAA TCGCTAAGTC TGGGGATACG TCTGCACCCC AAGAGGATGT GGGCACTGAG 
AGTTCAGAGA GTGGTTCTTC ACTGCAGGCC CCAAGTACAG AGCCTACCCC AGATGTGAAT 
CGGTCGCCAC ACAGTAAGCG TCTGGCCAAA GGCAGCCATC GTTCTCGAAA CAGCACTCTG 
ACTTCTCTGT GCATCCTTAG TCACTATCCT TTCTTCTCCA CCTTCCGCGA GTGTCTGTAC 
ACCCTCAAGA GACTTGTGGA CTGCTGTAGT GAGAGACTGT TGGGCAAGAA GCTGGGAATT 
CCTCGTGGGG TTCAGAGGGA TACAACGTGG CGAATTTTCA CAGGCTCACT CCTGGTGGAG 
GAGAAGTCCA GCACACTGCT ACACGACTTG CGGGAGATTG AGGCCTGGAT ATACCGGCTG 
CTGCGTTCGC CAATGCCTGT CGCTGGTCAG AAGCGGGTGG ACGTGGAAGT TTTGCCACAT 
GAATTGCAGC CAGCTTTGAC CTTTGCTCTT CCTGATCCAT CCCGCTTCAC CTTGGTGGAT 
TTTCCATTGC ATCTGCCTTT GGAGTTGCTG GGAGTGGATG CCTGTCTTCA GGTGCTGACC 
TGCATCCTTC TGGAGCACAA GGTTGTACTG CAGTCTCGGG ATTATAATGC GCTCTCAATG 
TCTGTGATGG CCTTTGTGGC CATGATCTAC CCATTAGAAT ATATGTTTCC GGTGATCCCT 
CTGCTTCCCA CCTGCATGGC CTCTGCAGAA CAGTTGCTTC TAGCCCCTAC ACCTTACATC 
ATTGGTGTTC CAGCAAGTTT CTTCCTTTAC AAACTAGATT TTAAAATGCC TGATGATGTA 
TGGCTTATTG ACCTGGATAC CAATAAGGTG ATCGTTCCCA CAAATGCAGA ATCTTTGCCA 
GCACTGCCAG AACCAGAAGC TTCAGAGCTG AAAAAGCATC TGAAACAGGC ACTGGCCAGC 
ATGAGTCTGA ATACTCAGCC CATTCTTAAT CTAGAGAAGT TCCACGAGGG GCAGGAGGTG 
CCACTGCTGC TGGGAAGACC ACAGAATGAT TTACAGTCTA CTCCCTCCAC GGAATTCAAC 
CCTCTGATCT ATGGAAATGA TGTAGACTCT GTGGATGTGG CTACCAGGGT TGCTATGGTG 
AGATTCTTCA ATTCACCAAA TGTTTTGCAA GGATTCCAGA TGCATACTCG CACTCTTCGT 
CTTTTTCCAC GACCAGTAGT GGCCTTCCAG GCAAATTCCT TTCTTGCTTC TAGGCCAAAG 
CAAACACCTT TTGCAGATAA GCTTTCCAGG ACGCAAGCAG TAGAATATTT TGGAGAATGG 
TCACTCAACC CTACTAACTA TGCTTTCCAA AGAATTCATA ACAATATGTT TGACCCAGCA 
CTGATTGGTG ACAAACCGAA GTGGTATGCT CACCAGCTGC AGCCCATCCA CTATCGTGTC 
TATGACAGTA ATTCACAACT AGCTGAAGCA CTGAATGTTC CAGTAGAAAA AGAAACGGAC 
TCTGACCCCA CTGATGACAG TGGCAGTGAC AGTGTCGACT ATGATGACTC AAGTTCCTCC 
TACTCTTCCC TTGGAGACTT TGTCAGTGAG ATGATGAAAT GTGACATTAA TGGCGATACT 
CCAAATGTTG ACCCCCTGAC TCACGCAGCC CTTGGTGATG CCAGTGAAGT GGAGTTCGAT 
GATTTTCAAG AATATTCAGG AGAGCTGGAT GAACAGGCCA TGGACAGCGA GAACTCCCAA 
GAGAACAATC AGCCTCGTTC AAGTTCCAGT ACTACAGCCA GTAGCAGCCC CAGTACCGTC 
ATCCATGGAG TGAATCATGA GTCTGCAGAT TCAGCAGAGA TGGAGGAGAA GCTAGTTGCC 
GGATTTTCAA ACCATCTCCC TTCTTTGCCA CTGCAACCAA GCTTTCCCAA GATGAGCTTT 
GATCGTCGTG AGAGTGATAG CCCAGCTGTC AGCATGAGCT CCTCAGAAGG GGTGGTGAGG 
AAACGAGAGT ATGATAATCC ATACTTCGAA CCACAGTATG GTTTTCCTAC AGAGGATGAA 
GATGATGAGC AGGAAGAGAG CTACACCCCA AGATTTAACC AGAATCTCAA TGGAAGCAGG 
TCTCAGAAGT TACTTCGTCC AAACAGTTTA AAACTGGCCA ATGATTCTGA TGCAGATTCA 
GACTCCAGGG CCAGCTCTCC AAACTCTACT GTCTCCAACA ACAGCAGTGA AGGTTTTGGG 
GGCATCATGT CTTTTGCAAG CAGCCTGTAC AGAAATCACA GCACAAGTTT CAGTCTGTCC 
AATTTAGCCC TGCCAACCAA AGTTGGGAGA GACAAGAATA CCCCCTTTCC CAGCCTGAAA 
GGAAACAGGA GAGCCCTGGT GGATCAAAAG TCTTCAGTCA TTAAGCATAG CCCAACAGTG 
AAGAGGGAAT CTCCATCACC TCAGGGACGA AGTAGTAACT CCAGTGAGAA CCAGCAATTC 
CTGAAGGAGG TGGTACACAA TGTTCTCGAT GGGCAAGGTG TTGGTTGGCT GAACATGAAG 
AGAGTCCGAC GTCTGCTGGA GAGTGAGCAG CTCCGTGTCT TTGTACTAAG CAAGCTAAAT 
CGCACGATCC AGTCGGAAGA AGATGCTCAG CAGGATATCA TACAGGATGT GGAGATCAGC 
CGCAAAGTTT ATAAAGGCAT GCTGGACTTG CTAAAGTGTA CTGTGCTGAG CCTGGAGCAG 
TCATACGCAA ATGCTGGCCT GGGAGGCATG GCCAGTGTTT TTGGCCTTCT AGAAATAGCA 
CATACTCACT ATTACAACAA AGAACCAGAA AAGAGAAAAC GAAGCCCAAC AGATGGATCT 
GTCACTCCGG TTGGCAAAGA TCCTGCATCA TCTCCAAGAG TGGAGCCGAA ACCTGCCATG 
CAGCTGCCAG TACCACAGCT GATGCCGAAG GCACCAAGCC CTGCAGGGAA AGGGCTAAGG 
GAGTTTGATA CAAGGAGTCT AAAGGAAGAA AATTTTATTG CTTCCATTGG GCCAGAAGGT 
GTGAAACATC TTGATTTGGG AGAAACAGAT GAGAAAAAAT CCCAAATCAG TGCAGACAGT 
GGCCTCAGTT TGGCCTCAGG TTCTCAGAAG AGTGATTTTG ACTCTGTTCC CAGTGGAGGA 
CCGTCAGTTA TGGTCCGAAG TACCAGCCAG GATTCTGAAG TTAGCACAGT GGTTAGTAAC 
AGCTCTGGGG AGACACTAGG AGCAGACAGT GACTTGAGTA GCAATGCTGG TGATGGCCCA 
AGTGTGGAAA ATGGAGGCAA TCTGGCAGGA TCCAGGGGCA CCGTGTCAGA CAGTGAAATT 
GAGACAAACT CTGCTACTAG CTCTATTTTC GCAAAGTCTC ATAACTTGAA GCAGAATGTA 
AAGGAGAACA AAGGCAGTAC TGCAGGGAAA GGTCCAGAGG ATGGGAACCA GCGTGTCTAT 
CTCTATGAAG GACTTTTGGG CAAAGAGCGC TCAACACTGT GGGACCAGAT GCAATTCTGG 
GAAGATGCCT TTTTGGATGC TGTGATGTTA GAGAGAGAAG GAATGGGGAT GGATCAGGGA 
CCCCAGGAGA TGATTGACAG GTATCTTTCC CTGGGAGAAC ATGATCGAAA ACGTTTGGAG 
GATGATGAGG ACCGCTTATT GGCTACGCTG CTACATAATA TGATTGCTTA TATGCTTATG 
ATAAAGGTGA ATAAGAATGA TATTAGGAAA AAGGTGCGTC GTCTGATGGG AAAATCGCAT 
ATTGGATTGT TGCACAGCCA GCAGTTAAAT GACATCCTAG ACAAGCTTGC TAACCTGAAT 
GGACGGGAAC TTCCTGTGAG ACCCAGCGGC AGCCGCCACA TCAAGAAGCA GACTTTTGTA 
GTCCATGCTG GGACAGACAC TACAGGAGAT ATATTTTTCA TGGAGGTTTG TGATGACTGC 
ATTGTGCTGA GAAGCAACAT TGGAACTGTG TATGAACGTT GGTGGTATGA GAAACTCATC 
AACATGACTT ACTGTCCCAA AACAAAAGTT CTGTGTCTCT GGCGGAGGAA TGGTCAGGAA 
ACACAACTGA ACAAGTTCTA CACAAAGAAG TGTCGGGAAT TATACTACTG CGTAAAAGAC 
AGCATGGAGC GAGCAGCAGC AAGACAGCAA AGCATTAAGC CAGGCCCTGA GTTGGGTGGT 
GAGTTTCCTG TGCAGGATAT GAAAACTGGC GAAGGAGGTC TTCTGCAGGT GACACTGGAA 
GGAATTAACC TGAAATTTAT GCACAGTCAG TTCCTGAAAC TAAAGAAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

MADD cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: