LETM1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	LETM1
Entrez Gene ID	422898
Full Name	leucine zipper and EF-hand containing transmembrane protein 1
General protein information	Preferred Names leucine zipper and EF-hand containing transmembrane protein 1 Names mitochondrial proton/calcium exchanger protein LETM1 and EF-hand domain-containing protein 1, mitochondrial leucine zipper-EF-hand-containing transmembrane protein 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015285900.2	XP_015141386.1	mitochondrial proton/calcium exchanger protein isoform X1
XM_015285900.1	XP_015141386.1	LETM1 and EF-hand domain-containing protein 1, mitochondrial isoform X1
NM_001006461.2	NP_001006461.2	mitochondrial proton/calcium exchanger protein
NM_001006461.3	NP_001006461.2	mitochondrial proton/calcium exchanger protein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LETM1	NP_001069082.1
Mus musculus (house mouse)	Letm1	NP_062668.1
Danio rerio (zebrafish)	letm1	NP_001038673.1
Kluyveromyces lactis	KLLA0B11341g	XP_452034.1
Neurospora crassa	NCU03217	XP_965317.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003296	XP_003436436.1
Caenorhabditis elegans (roundworm)	letm-1	NP_506381.1
Saccharomyces cerevisiae (baker's yeast)	YLH47	NP_015450.1
Eremothecium gossypii	AGOS_AAR032W	NP_982573.2
Xenopus tropicalis (tropical clawed frog)	letm1	NP_001072793.1
Canis lupus familiaris (dog)	LETM1	XP_545925.2
Rattus norvegicus (Norway rat)	Letm1	NP_001005884.1
Magnaporthe oryzae (rice blast fungus)	MGG_03684	XP_003716197.1
Homo sapiens (human)	LETM1	NP_036450.1
Gallus gallus (chicken)	LETM1	NP_001006461.2
Pan troglodytes (chimpanzee)	LETM1	XP_003310276.1
	LETM1	XP_002802216.1
Schizosaccharomyces pombe (fission yeast)	mdm28	NP_593648.1

Gene Ontology
Bibliography

Related articles in PubMed

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Caldwell RB, Kierzek AM, Arakawa H, Bezzubov Y, Zaim J, Fiedler P, Kutter S, Blagodatski A, Kostovska D, Koter M, Plachy J, Carninci P, Hayashizaki Y, Buerstedde JM
Genome biology6(1)R6(2005)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LETM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LETM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa28182	NM_001006461.3 Latest version!	Gallus gallus leucine zipper and EF-hand containing transmembrane protein 1 (LETM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OGa35807	XM_015285900.2 Latest version!	Gallus gallus leucine zipper and EF-hand containing transmembrane protein 1 (LETM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
View Old Accession Versions >>
OGa35807	XM_015285900.1	Gallus gallus leucine zipper-EF-hand containing transmembrane protein 1 (LETM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OGa28182	NM_001006461.2	Gallus gallus leucine zipper and EF-hand containing transmembrane protein 1 (LETM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LETM1 ( NM_001006461.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001006461.2 Gallus gallus leucine zipper and EF-hand containing transmembrane protein 1 (LETM1), mRNA.

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CloneID	OGa28182
Clone ID Related Accession (Same CDS sequence)	NM_001006461.2 , NM_001006461.3
Accession Version	NM_001006461.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-01
Organism	Gallus gallus(chicken)
Product	mitochondrial proton/calcium exchanger protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AJ720251.1 and AADN04000201.1. On Sep 28, 2011 this sequence version replaced NM_001006461.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## Transcript exon combination :: AJ720251.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2201361, SAMEA2201363 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCCA TGCTGCTACA GAGCTGCGGC CGGCGGGCCT GCCGCCTGCC CCGCGCGCTG 
CGCCGCCAGG CCCCGCTGGG TAACTTGGGA GATCGCGCTT GTCTTAGTTG TACATCCCTG 
AGGTTGGCAA ACAAAATGAC TGTACACTTT AAATATTGCA CTAGTGTTGC TCCTGTTTAC 
GCATACTCCA AAAAAGATCA CTACTGTTGT TGGACTAAAG GATTGGAACG AACGTACTTT 
ACTCTTATGA GCTCTTCTGG CTCATGGACG CCCCTGGCAG CCATGGGTGT TTTAGGACCC 
AAATATCTCC CAGTTAGGTG GTGGCATTCT TCACGTTCTC TTCAAGATGA CTCCATAGTT 
GAAAAGTCCC TGAAGTCCTT AAAGGACAAA AACAAGAAGT TGGAAGAAGG GGGTCCTGTA 
TATAGTCCAA CAGAAGTGGA AGTTGTGAAA AAATCTATTG GACAGAGGAT TGTGGATGAG 
CTGAAGCACT ATTACCATGG TTTTCGATTA CTCTGGATTG ACACAAAAAT AGCTGCAAGG 
ATGCTTTGGC GAATACTACA TGGAAATACT CTGTCACGTC GAGAACGGAG ACAGTTTCTT 
CGAATATGTG CTGATCTCTT CCGCTTGGTT CCTTTCCTCG TTTTTCTCGT TGTCCCATTT 
ATGGAGTTTC TGCTTCCAGT AGCTCTGAAG TTGTTCCCTA ATATGCTTCC GTCTACATTT 
GAGACAAAGT CTAAAAAGGA GGAAAGATTG AAGAAACAAC TGAGAGTAAA GCTTGAATTA 
GCTAAATTCC TTCAAGATAC AATTGAGGAG ATGGCCTTAA AAAATAAGGC AGCCAAAGGA 
AATGTTACAA AAGATTTTTC AACGTTCTTT CAAAAGATCA GGGAGACTGG TGAAAGACCC 
AGTAATGAGG AGATCCTGAG GTTCTCAAAA CTATTTGAAG ATGAATTGAC TCTGGACAAT 
CTAACCAGGC CTCAGTTGGT GGCACTTTGT AAATTGTTGG AACTTCAGTC AATTGGGACG 
AATAACTTTC TCCGCTTCCA GCTGACTATG AGGTTGAGAA CTATAAAAGC AGATGACAAA 
ATGATTGCTG AAGAAGGAGT TGACAGCCTG ACTGTTAAAG AACTGCAGGC AGCCTGTCGT 
GCACGAGGAA TGAGAGCCCT TGGTGTGACA GAAGAGCGCC TCAGGGAACA GCTTAAACAG 
TGGCTAGATC TACACTTGAA CCAGGAAATC CCTACTTCAT TGCTCATTTT ATCCAGAGCC 
ATGTATCTTC CAGACACGCT TTCTCCAGCT GATCAGCTCA AAACAACGCT TCAGACACTA 
CCAGAGAGTG TTGCCAAAGA GGCTCAAGTC AAAGTGGCGG AAGTTGAAGG TGAAAAAGTA 
GATAACAAAG CACGGCTGGA GGCAACGCTT CAGGAAGAAG CAGCCATCAG AAAAGAAAAT 
GAGGAAAAAG AGATGGAAAG AATAACTGAA GCTGCAGAGA AAGCCAAAGA AACTCTTCAG 
GTTGCAGCCA TGAAATTGGA ATCAGCAGTA GATCTTGAGC CAGCTGTTCT GCAAGTTAAA 
GAAAGTCAGG TGGCTATGGA CAGCAAACAA GAGCTGGCAA AAGCAGATAT GGAAACGTTG 
AAAGATACAG CACCCGTACT GGAAGGCATT AAGGGTGAGG AAATAACTAA GGAGGAGATT 
GACATGCTGA GTGATGCTTG TAACAAGCTG CAGGAACAGA AGAAATCACT AACAAAGGAA 
AAGGAGGAGC TTGAGGAACT GAAGGGTGAT ATCCAGGAAT ATAATGAAGA TTTGCAAGAA 
ATAAAGGAGC TTTCTAAAGC TGGCCAGGAA GAAGTAGTAG AAGAATCAAA GGCGAGCAAG 
CGACTGACCA AAAGAGTGAA CCGGATGATT GGTCAGATAG ACAAAATTAT TAATGAGTTA 
GAGACGAATC AAAAGACGGT GGATGTAAAG CTTGATAGAG GTGACAGTCC TCCTGCTGGG 
GAGAATCTAA TCAGTATTGC TGAGCTAATT AATGCAATGA AACAAATCCA GAAGATTCCA 
GAGGAGAAGC TAACGAGGAT TGCAGAGGCA TTAGATGAAA ACAAAGATGG CAAAATTGAT 
ATAGATAATG TTGTTAAGGT AGTAGAATTG ATAGACAAAG AAGATGTTGA TATTGGCACC 
AGCCAGGTCG CTGAGATTAT GGCACTGCTT CAAAAAGAAG AAAAACTGGA AGAAAAGGAG 
AAAGCAAAGG AAAAGCAAGA TAAAGAAGCT GCAGAAGTAA AGAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001006461.2
CDS	89..2356
Translation	MASMLLQSCGRRACRLPRALRRQAPLGNLGDRACLSCTSLRLANKMTVHFKYCTSVAPVYAYSKKDHYCCWTKGLERTYFTLMSSSGSWTPLAAMGVLGPKYLPVRWWHSSRSLQDDSIVEKSLKSLKDKNKKLEEGGPVYSPTEVEVVKKSIGQRIVDELKHYYHGFRLLWIDTKIAARMLWRILHGNTLSRRERRQFLRICADLFRLVPFLVFLVVPFMEFLLPVALKLFPNMLPSTFETKSKKEERLKKQLRVKLELAKFLQDTIEEMALKNKAAKGNVTKDFSTFFQKIRETGERPSNEEILRFSKLFEDELTLDNLTRPQLVALCKLLELQSIGTNNFLRFQLTMRLRTIKADDKMIAEEGVDSLTVKELQAACRARGMRALGVTEERLREQLKQWLDLHLNQEIPTSLLILSRAMYLPDTLSPADQLKTTLQTLPESVAKEAQVKVAEVEGEKVDNKARLEATLQEEAAIRKENEEKEMERITEAAEKAKETLQVAAMKLESAVDLEPAVLQVKESQVAMDSKQELAKADMETLKDTAPVLEGIKGEEITKEEIDMLSDACNKLQEQKKSLTKEKEELEELKGDIQEYNEDLQEIKELSKAGQEEVVEESKASKRLTKRVNRMIGQIDKIINELETNQKTVDVKLDRGDSPPAGENLISIAELINAMKQIQKIPEEKLTRIAEALDENKDGKIDIDNVVKVVELIDKEDVDIGTSQVAEIMALLQKEEKLEEKEKAKEKQDKEAAEVKN

Target ORF information:

RefSeq Version	NM_001006461.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus leucine zipper and EF-hand containing transmembrane protein 1 (LETM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001006461.2

ORF Insert Sequence:

ATGGCGTCCA TGCTGCTACA GAGCTGCGGC CGGCGGGCCT GCCGCCTGCC CCGCGCGCTG 
CGCCGCCAGG CCCCGCTGGG TAACTTGGGA GATCGCGCTT GTCTTAGTTG TACATCCCTG 
AGGTTGGCAA ACAAAATGAC TGTACACTTT AAATATTGCA CTAGTGTTGC TCCTGTTTAC 
GCATACTCCA AAAAAGATCA CTACTGTTGT TGGACTAAAG GATTGGAACG AACGTACTTT 
ACTCTTATGA GCTCTTCTGG CTCATGGACG CCCCTGGCAG CCATGGGTGT TTTAGGACCC 
AAATATCTCC CAGTTAGGTG GTGGCATTCT TCACGTTCTC TTCAAGATGA CTCCATAGTT 
GAAAAGTCCC TGAAGTCCTT AAAGGACAAA AACAAGAAGT TGGAAGAAGG GGGTCCTGTA 
TATAGTCCAA CAGAAGTGGA AGTTGTGAAA AAATCTATTG GACAGAGGAT TGTGGATGAG 
CTGAAGCACT ATTACCATGG TTTTCGATTA CTCTGGATTG ACACAAAAAT AGCTGCAAGG 
ATGCTTTGGC GAATACTACA TGGAAATACT CTGTCACGTC GAGAACGGAG ACAGTTTCTT 
CGAATATGTG CTGATCTCTT CCGCTTGGTT CCTTTCCTCG TTTTTCTCGT TGTCCCATTT 
ATGGAGTTTC TGCTTCCAGT AGCTCTGAAG TTGTTCCCTA ATATGCTTCC GTCTACATTT 
GAGACAAAGT CTAAAAAGGA GGAAAGATTG AAGAAACAAC TGAGAGTAAA GCTTGAATTA 
GCTAAATTCC TTCAAGATAC AATTGAGGAG ATGGCCTTAA AAAATAAGGC AGCCAAAGGA 
AATGTTACAA AAGATTTTTC AACGTTCTTT CAAAAGATCA GGGAGACTGG TGAAAGACCC 
AGTAATGAGG AGATCCTGAG GTTCTCAAAA CTATTTGAAG ATGAATTGAC TCTGGACAAT 
CTAACCAGGC CTCAGTTGGT GGCACTTTGT AAATTGTTGG AACTTCAGTC AATTGGGACG 
AATAACTTTC TCCGCTTCCA GCTGACTATG AGGTTGAGAA CTATAAAAGC AGATGACAAA 
ATGATTGCTG AAGAAGGAGT TGACAGCCTG ACTGTTAAAG AACTGCAGGC AGCCTGTCGT 
GCACGAGGAA TGAGAGCCCT TGGTGTGACA GAAGAGCGCC TCAGGGAACA GCTTAAACAG 
TGGCTAGATC TACACTTGAA CCAGGAAATC CCTACTTCAT TGCTCATTTT ATCCAGAGCC 
ATGTATCTTC CAGACACGCT TTCTCCAGCT GATCAGCTCA AAACAACGCT TCAGACACTA 
CCAGAGAGTG TTGCCAAAGA GGCTCAAGTC AAAGTGGCGG AAGTTGAAGG TGAAAAAGTA 
GATAACAAAG CACGGCTGGA GGCAACGCTT CAGGAAGAAG CAGCCATCAG AAAAGAAAAT 
GAGGAAAAAG AGATGGAAAG AATAACTGAA GCTGCAGAGA AAGCCAAAGA AACTCTTCAG 
GTTGCAGCCA TGAAATTGGA ATCAGCAGTA GATCTTGAGC CAGCTGTTCT GCAAGTTAAA 
GAAAGTCAGG TGGCTATGGA CAGCAAACAA GAGCTGGCAA AAGCAGATAT GGAAACGTTG 
AAAGATACAG CACCCGTACT GGAAGGCATT AAGGGTGAGG AAATAACTAA GGAGGAGATT 
GACATGCTGA GTGATGCTTG TAACAAGCTG CAGGAACAGA AGAAATCACT AACAAAGGAA 
AAGGAGGAGC TTGAGGAACT GAAGGGTGAT ATCCAGGAAT ATAATGAAGA TTTGCAAGAA 
ATAAAGGAGC TTTCTAAAGC TGGCCAGGAA GAAGTAGTAG AAGAATCAAA GGCGAGCAAG 
CGACTGACCA AAAGAGTGAA CCGGATGATT GGTCAGATAG ACAAAATTAT TAATGAGTTA 
GAGACGAATC AAAAGACGGT GGATGTAAAG CTTGATAGAG GTGACAGTCC TCCTGCTGGG 
GAGAATCTAA TCAGTATTGC TGAGCTAATT AATGCAATGA AACAAATCCA GAAGATTCCA 
GAGGAGAAGC TAACGAGGAT TGCAGAGGCA TTAGATGAAA ACAAAGATGG CAAAATTGAT 
ATAGATAATG TTGTTAAGGT AGTAGAATTG ATAGACAAAG AAGATGTTGA TATTGGCACC 
AGCCAGGTCG CTGAGATTAT GGCACTGCTT CAAAAAGAAG AAAAACTGGA AGAAAAGGAG 
AAAGCAAAGG AAAAGCAAGA TAAAGAAGCT GCAGAAGTAA AGAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LETM1 ( NM_001006461.3 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001006461.2 Gallus gallus leucine zipper and EF-hand containing transmembrane protein 1 (LETM1), mRNA.

Price & Availability↑

CloneID	OGa28182
Clone ID Related Accession (Same CDS sequence)	NM_001006461.2 , NM_001006461.3
Accession Version	NM_001006461.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-19
Organism	Gallus gallus(chicken)
Product	mitochondrial proton/calcium exchanger protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000017.1. On Dec 20, 2018 this sequence version replaced NM_001006461.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## Transcript exon combination :: AJ720251.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2201361, SAMEA2201363 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCCA TGCTGCTACA GAGCTGCGGC CGGCGGGCCT GCCGCCTGCC CCGCGCGCTG 
CGCCGCCAGG CCCCGCTGGG TAACTTGGGA GATCGCGCTT GTCTTAGTTG TACATCCCTG 
AGGTTGGCAA ACAAAATGAC TGTACACTTT AAATATTGCA CTAGTGTTGC TCCTGTTTAC 
GCATACTCCA AAAAAGATCA CTACTGTTGT TGGACTAAAG GATTGGAACG AACGTACTTT 
ACTCTTATGA GCTCTTCTGG CTCATGGACG CCCCTGGCAG CCATGGGTGT TTTAGGACCC 
AAATATCTCC CAGTTAGGTG GTGGCATTCT TCACGTTCTC TTCAAGATGA CTCCATAGTT 
GAAAAGTCCC TGAAGTCCTT AAAGGACAAA AACAAGAAGT TGGAAGAAGG GGGTCCTGTA 
TATAGTCCAA CAGAAGTGGA AGTTGTGAAA AAATCTATTG GACAGAGGAT TGTGGATGAG 
CTGAAGCACT ATTACCATGG TTTTCGATTA CTCTGGATTG ACACAAAAAT AGCTGCAAGG 
ATGCTTTGGC GAATACTACA TGGAAATACT CTGTCACGTC GAGAACGGAG ACAGTTTCTT 
CGAATATGTG CTGATCTCTT CCGCTTGGTT CCTTTCCTCG TTTTTCTCGT TGTCCCATTT 
ATGGAGTTTC TGCTTCCAGT AGCTCTGAAG TTGTTCCCTA ATATGCTTCC GTCTACATTT 
GAGACAAAGT CTAAAAAGGA GGAAAGATTG AAGAAACAAC TGAGAGTAAA GCTTGAATTA 
GCTAAATTCC TTCAAGATAC AATTGAGGAG ATGGCCTTAA AAAATAAGGC AGCCAAAGGA 
AATGTTACAA AAGATTTTTC AACGTTCTTT CAAAAGATCA GGGAGACTGG TGAAAGACCC 
AGTAATGAGG AGATCCTGAG GTTCTCAAAA CTATTTGAAG ATGAATTGAC TCTGGACAAT 
CTAACCAGGC CTCAGTTGGT GGCACTTTGT AAATTGTTGG AACTTCAGTC AATTGGGACG 
AATAACTTTC TCCGCTTCCA GCTGACTATG AGGTTGAGAA CTATAAAAGC AGATGACAAA 
ATGATTGCTG AAGAAGGAGT TGACAGCCTG ACTGTTAAAG AACTGCAGGC AGCCTGTCGT 
GCACGAGGAA TGAGAGCCCT TGGTGTGACA GAAGAGCGCC TCAGGGAACA GCTTAAACAG 
TGGCTAGATC TACACTTGAA CCAGGAAATC CCTACTTCAT TGCTCATTTT ATCCAGAGCC 
ATGTATCTTC CAGACACGCT TTCTCCAGCT GATCAGCTCA AAACAACGCT TCAGACACTA 
CCAGAGAGTG TTGCCAAAGA GGCTCAAGTC AAAGTGGCGG AAGTTGAAGG TGAAAAAGTA 
GATAACAAAG CACGGCTGGA GGCAACGCTT CAGGAAGAAG CAGCCATCAG AAAAGAAAAT 
GAGGAAAAAG AGATGGAAAG AATAACTGAA GCTGCAGAGA AAGCCAAAGA AACTCTTCAG 
GTTGCAGCCA TGAAATTGGA ATCAGCAGTA GATCTTGAGC CAGCTGTTCT GCAAGTTAAA 
GAAAGTCAGG TGGCTATGGA CAGCAAACAA GAGCTGGCAA AAGCAGATAT GGAAACGTTG 
AAAGATACAG CACCCGTACT GGAAGGCATT AAGGGTGAGG AAATAACTAA GGAGGAGATT 
GACATGCTGA GTGATGCTTG TAACAAGCTG CAGGAACAGA AGAAATCACT AACAAAGGAA 
AAGGAGGAGC TTGAGGAACT GAAGGGTGAT ATCCAGGAAT ATAATGAAGA TTTGCAAGAA 
ATAAAGGAGC TTTCTAAAGC TGGCCAGGAA GAAGTAGTAG AAGAATCAAA GGCGAGCAAG 
CGACTGACCA AAAGAGTGAA CCGGATGATT GGTCAGATAG ACAAAATTAT TAATGAGTTA 
GAGACGAATC AAAAGACGGT GGATGTAAAG CTTGATAGAG GTGACAGTCC TCCTGCTGGG 
GAGAATCTAA TCAGTATTGC TGAGCTAATT AATGCAATGA AACAAATCCA GAAGATTCCA 
GAGGAGAAGC TAACGAGGAT TGCAGAGGCA TTAGATGAAA ACAAAGATGG CAAAATTGAT 
ATAGATAATG TTGTTAAGGT AGTAGAATTG ATAGACAAAG AAGATGTTGA TATTGGCACC 
AGCCAGGTCG CTGAGATTAT GGCACTGCTT CAAAAAGAAG AAAAACTGGA AGAAAAGGAG 
AAAGCAAAGG AAAAGCAAGA TAAAGAAGCT GCAGAAGTAA AGAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001006461.2
CDS	89..2356
Translation	MASMLLQSCGRRACRLPRALRRQAPLGNLGDRACLSCTSLRLANKMTVHFKYCTSVAPVYAYSKKDHYCCWTKGLERTYFTLMSSSGSWTPLAAMGVLGPKYLPVRWWHSSRSLQDDSIVEKSLKSLKDKNKKLEEGGPVYSPTEVEVVKKSIGQRIVDELKHYYHGFRLLWIDTKIAARMLWRILHGNTLSRRERRQFLRICADLFRLVPFLVFLVVPFMEFLLPVALKLFPNMLPSTFETKSKKEERLKKQLRVKLELAKFLQDTIEEMALKNKAAKGNVTKDFSTFFQKIRETGERPSNEEILRFSKLFEDELTLDNLTRPQLVALCKLLELQSIGTNNFLRFQLTMRLRTIKADDKMIAEEGVDSLTVKELQAACRARGMRALGVTEERLREQLKQWLDLHLNQEIPTSLLILSRAMYLPDTLSPADQLKTTLQTLPESVAKEAQVKVAEVEGEKVDNKARLEATLQEEAAIRKENEEKEMERITEAAEKAKETLQVAAMKLESAVDLEPAVLQVKESQVAMDSKQELAKADMETLKDTAPVLEGIKGEEITKEEIDMLSDACNKLQEQKKSLTKEKEELEELKGDIQEYNEDLQEIKELSKAGQEEVVEESKASKRLTKRVNRMIGQIDKIINELETNQKTVDVKLDRGDSPPAGENLISIAELINAMKQIQKIPEEKLTRIAEALDENKDGKIDIDNVVKVVELIDKEDVDIGTSQVAEIMALLQKEEKLEEKEKAKEKQDKEAAEVKN

Target ORF information:

RefSeq Version	NM_001006461.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus leucine zipper and EF-hand containing transmembrane protein 1 (LETM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001006461.3

ORF Insert Sequence:

ATGGCGTCCA TGCTGCTACA GAGCTGCGGC CGGCGGGCCT GCCGCCTGCC CCGCGCGCTG 
CGCCGCCAGG CCCCGCTGGG TAACTTGGGA GATCGCGCTT GTCTTAGTTG TACATCCCTG 
AGGTTGGCAA ACAAAATGAC TGTACACTTT AAATATTGCA CTAGTGTTGC TCCTGTTTAC 
GCATACTCCA AAAAAGATCA CTACTGTTGT TGGACTAAAG GATTGGAACG AACGTACTTT 
ACTCTTATGA GCTCTTCTGG CTCATGGACG CCCCTGGCAG CCATGGGTGT TTTAGGACCC 
AAATATCTCC CAGTTAGGTG GTGGCATTCT TCACGTTCTC TTCAAGATGA CTCCATAGTT 
GAAAAGTCCC TGAAGTCCTT AAAGGACAAA AACAAGAAGT TGGAAGAAGG GGGTCCTGTA 
TATAGTCCAA CAGAAGTGGA AGTTGTGAAA AAATCTATTG GACAGAGGAT TGTGGATGAG 
CTGAAGCACT ATTACCATGG TTTTCGATTA CTCTGGATTG ACACAAAAAT AGCTGCAAGG 
ATGCTTTGGC GAATACTACA TGGAAATACT CTGTCACGTC GAGAACGGAG ACAGTTTCTT 
CGAATATGTG CTGATCTCTT CCGCTTGGTT CCTTTCCTCG TTTTTCTCGT TGTCCCATTT 
ATGGAGTTTC TGCTTCCAGT AGCTCTGAAG TTGTTCCCTA ATATGCTTCC GTCTACATTT 
GAGACAAAGT CTAAAAAGGA GGAAAGATTG AAGAAACAAC TGAGAGTAAA GCTTGAATTA 
GCTAAATTCC TTCAAGATAC AATTGAGGAG ATGGCCTTAA AAAATAAGGC AGCCAAAGGA 
AATGTTACAA AAGATTTTTC AACGTTCTTT CAAAAGATCA GGGAGACTGG TGAAAGACCC 
AGTAATGAGG AGATCCTGAG GTTCTCAAAA CTATTTGAAG ATGAATTGAC TCTGGACAAT 
CTAACCAGGC CTCAGTTGGT GGCACTTTGT AAATTGTTGG AACTTCAGTC AATTGGGACG 
AATAACTTTC TCCGCTTCCA GCTGACTATG AGGTTGAGAA CTATAAAAGC AGATGACAAA 
ATGATTGCTG AAGAAGGAGT TGACAGCCTG ACTGTTAAAG AACTGCAGGC AGCCTGTCGT 
GCACGAGGAA TGAGAGCCCT TGGTGTGACA GAAGAGCGCC TCAGGGAACA GCTTAAACAG 
TGGCTAGATC TACACTTGAA CCAGGAAATC CCTACTTCAT TGCTCATTTT ATCCAGAGCC 
ATGTATCTTC CAGACACGCT TTCTCCAGCT GATCAGCTCA AAACAACGCT TCAGACACTA 
CCAGAGAGTG TTGCCAAAGA GGCTCAAGTC AAAGTGGCGG AAGTTGAAGG TGAAAAAGTA 
GATAACAAAG CACGGCTGGA GGCAACGCTT CAGGAAGAAG CAGCCATCAG AAAAGAAAAT 
GAGGAAAAAG AGATGGAAAG AATAACTGAA GCTGCAGAGA AAGCCAAAGA AACTCTTCAG 
GTTGCAGCCA TGAAATTGGA ATCAGCAGTA GATCTTGAGC CAGCTGTTCT GCAAGTTAAA 
GAAAGTCAGG TGGCTATGGA CAGCAAACAA GAGCTGGCAA AAGCAGATAT GGAAACGTTG 
AAAGATACAG CACCCGTACT GGAAGGCATT AAGGGTGAGG AAATAACTAA GGAGGAGATT 
GACATGCTGA GTGATGCTTG TAACAAGCTG CAGGAACAGA AGAAATCACT AACAAAGGAA 
AAGGAGGAGC TTGAGGAACT GAAGGGTGAT ATCCAGGAAT ATAATGAAGA TTTGCAAGAA 
ATAAAGGAGC TTTCTAAAGC TGGCCAGGAA GAAGTAGTAG AAGAATCAAA GGCGAGCAAG 
CGACTGACCA AAAGAGTGAA CCGGATGATT GGTCAGATAG ACAAAATTAT TAATGAGTTA 
GAGACGAATC AAAAGACGGT GGATGTAAAG CTTGATAGAG GTGACAGTCC TCCTGCTGGG 
GAGAATCTAA TCAGTATTGC TGAGCTAATT AATGCAATGA AACAAATCCA GAAGATTCCA 
GAGGAGAAGC TAACGAGGAT TGCAGAGGCA TTAGATGAAA ACAAAGATGG CAAAATTGAT 
ATAGATAATG TTGTTAAGGT AGTAGAATTG ATAGACAAAG AAGATGTTGA TATTGGCACC 
AGCCAGGTCG CTGAGATTAT GGCACTGCTT CAAAAAGAAG AAAAACTGGA AGAAAAGGAG 
AAAGCAAAGG AAAAGCAAGA TAAAGAAGCT GCAGAAGTAA AGAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LETM1 ( XM_015285900.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015141386.1 Gallus gallus leucine zipper and EF-hand containing transmembrane protein 1 (LETM1), transcript variant X1, mRNA.

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CloneID	OGa35807
Clone ID Related Accession (Same CDS sequence)	XM_015285900.2 , XM_015285900.1
Accession Version	XM_015285900.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	mitochondrial proton/calcium exchanger protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285900.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCA TGCTGCTACA GAGCTGCGGC CGGCGGGCCT GCCGCCTGCC CCGCGCGCTG 
CGCCGCCAGG CCCCGCTGGG TAACTTGGGA GATCGCGCTT GTCTTAGTTG TACATCCCTG 
AGGTTGGCAA ACAAAATGAC TGTACACTTT AAATATTGCA CTAGTGTTGC TCCTGTTTAC 
GCATACTCCA AAAAAGATCA CTACTGTTGT TGGACTAAAG GATTGGAACG AACGTACTTT 
ACTCTTATGA GCTCTTCTGG CTCATGGACG CCCCTGGCAG CCATGGGTGT TTTAGGACCC 
AAATATCTCC CAGTTAGGTG GTGGCATTCT TCACGTTCTC TTCAAGATGA CTCCATAGTT 
GAAAAGTCCC TGAAGTCCTT AAAGGACAAA AACAAGAAGT TGGAAGAAGG GGGTCCTGTA 
TATAGTCCAA CAGAAGTGGA AGTTGTGAAA AAATCTATTG GACAGAGGAT TGTGGATGAG 
CTGAAGCACT ATTACCATGG TTTTCGATTA CTCTGGATTG ACACAAAAAT AGCTGCAAGG 
ATGCTTTGGC GAATACTACA TGGAAATACT CTGTCACGTC GAGAACGGAG ACAGTTTCTT 
CGAATATGTG CTGATCTCTT CCGCTTGGTT CCTTTCCTCG TTTTTCTCGT TGTCCCATTT 
ATGGAGTTTC TGCTTCCAGT AGCTCTGAAG TTGTTCCCTA ATATGCTTCC GTCTACATTT 
GAGACAAAGT CTAAAAAGGA GGAAAGATTG AAGAAACAAC TGAGAGTAAA GCTTGAATTA 
GCTAAATTCC TTCAAGATAC AATTGAGGAG ATGGCCTTAA AAAATAAGGC AGCCAAAGGA 
AATGTTACAA AAGATTTTTC AACGTTCTTT CAAAAGATCA GGGAGACTGG TGAAAGACCC 
AGTAATGAGG AGATCCTGAG GTTCTCAAAA CTATTTGAAG ATGAATTGAC TCTGGACAAT 
CTAACCAGGC CTCAGTTGGT GGCACTTTGT AAATTGTTGG AACTTCAGTC AATTGGGACG 
AATAACTTTC TCCGCTTCCA GCTGACTATG AGGTTGAGAA CTATAAAAGC AGATGACAAA 
ATGATTGCTG AAGAAGGAGT TGACAGCCTG ACTGTTAAAG AACTGCAGGC AGCCTGTCGT 
GCACGAGGAA TGAGAGCCCT TGGTGTGACA GAAGAGCGCC TCAGGGAACA GCTTAAACAG 
TGGCTAGATC TACACTTGAA CCAGGAAATC CCTACTTCAT TGCTCATTTT ATCCAGAGCC 
ATGTATCTTC CAGACACGCT TTCTCCAGCT GATCAGCTCA AAACAACGCT TCAGACACTA 
CCAGAGAGTG TTGCCAAAGA GGCTCAAGTC AAAGTGGCGG AAGTTGAAGG TGAAAAAGTA 
GATAACAAAG CACGGCTGGA GGCAACGCTT CAGGAAGAAG CAGCCATCAG AAAAGAAAAT 
GAGGAAAAAG AGATGGAAAG AATAACTGAA GCTGCAGAGA AAGCCAAAGA AACTCTTCAG 
GTTGCAGCCA TGAAATTGGA ATCAGCAGTA GATCTTGAGC CAGCTGTTCT GCAAGTTAAA 
GAAAGTCAGG TGGCTATGGA CAGCAAACAA GAGCTGGCAA AAGCAGATAT GGAAACGTTG 
AAAGATACAG CACCCGTACT GGAAGGCATT AAGGGTGAGG AAATAACTAA GGAGGAGATT 
GACATGCTGA GTGATGCTTG TAACAAGCTG CAGGAACAGA AGAAATCACT AACAAAGGAA 
AAGGAGGAGC TTGAGGAACT GAAGGGTGAT ATCCAGGAAT ATAATGAAGA TTTGCAAGAA 
ATAAAGGAGC TTTCTAAAGC TGGCCAGGAA GAAGTAGTAG AAGAATCAAA GGCGAGCAAG 
CGACTGACCA AAAGAGTGAA CCGGATGATT GGTCAGATAG ACAAAATTAT TAATGAGTTA 
GAGACGAATC AAAAGACGGT GGATGTAAAG CTTGATAGAG GTGACAGTCC TCCTGCTGGA 
TTGTGCTTCC TCTTTAGGGA GAATCTAATC AGTATTGCTG AGCTAATTAA TGCAATGAAA 
CAAATCCAGA AGATTCCAGA GGAGAAGCTA ACGAGGATTG CAGAGGCATT AGATGAAAAC 
AAAGATGGCA AAATTGATAT AGATAATGTT GTTAAGGTAG TAGAATTGAT AGACAAAGAA 
GATGTTGATA TTGGCACCAG CCAGGTCGCT GAGATTATGG CACTGCTTCA AAAAGAAGAA 
AAACTGGAAG AAAAGGAGAA AGCAAAGGAA AAGCAAGATA AAGAAGCTGC AGAAGTAAAG 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015141386.1
CDS	130..2415
Translation	MASMLLQSCGRRACRLPRALRRQAPLGNLGDRACLSCTSLRLANKMTVHFKYCTSVAPVYAYSKKDHYCCWTKGLERTYFTLMSSSGSWTPLAAMGVLGPKYLPVRWWHSSRSLQDDSIVEKSLKSLKDKNKKLEEGGPVYSPTEVEVVKKSIGQRIVDELKHYYHGFRLLWIDTKIAARMLWRILHGNTLSRRERRQFLRICADLFRLVPFLVFLVVPFMEFLLPVALKLFPNMLPSTFETKSKKEERLKKQLRVKLELAKFLQDTIEEMALKNKAAKGNVTKDFSTFFQKIRETGERPSNEEILRFSKLFEDELTLDNLTRPQLVALCKLLELQSIGTNNFLRFQLTMRLRTIKADDKMIAEEGVDSLTVKELQAACRARGMRALGVTEERLREQLKQWLDLHLNQEIPTSLLILSRAMYLPDTLSPADQLKTTLQTLPESVAKEAQVKVAEVEGEKVDNKARLEATLQEEAAIRKENEEKEMERITEAAEKAKETLQVAAMKLESAVDLEPAVLQVKESQVAMDSKQELAKADMETLKDTAPVLEGIKGEEITKEEIDMLSDACNKLQEQKKSLTKEKEELEELKGDIQEYNEDLQEIKELSKAGQEEVVEESKASKRLTKRVNRMIGQIDKIINELETNQKTVDVKLDRGDSPPAGLCFLFRENLISIAELINAMKQIQKIPEEKLTRIAEALDENKDGKIDIDNVVKVVELIDKEDVDIGTSQVAEIMALLQKEEKLEEKEKAKEKQDKEAAEVKN

Target ORF information:

RefSeq Version	XM_015285900.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus leucine zipper and EF-hand containing transmembrane protein 1 (LETM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285900.2

ORF Insert Sequence:

ATGGCGTCCA TGCTGCTACA GAGCTGCGGC CGGCGGGCCT GCCGCCTGCC CCGCGCGCTG 
CGCCGCCAGG CCCCGCTGGG TAACTTGGGA GATCGCGCTT GTCTTAGTTG TACATCCCTG 
AGGTTGGCAA ACAAAATGAC TGTACACTTT AAATATTGCA CTAGTGTTGC TCCTGTTTAC 
GCATACTCCA AAAAAGATCA CTACTGTTGT TGGACTAAAG GATTGGAACG AACGTACTTT 
ACTCTTATGA GCTCTTCTGG CTCATGGACG CCCCTGGCAG CCATGGGTGT TTTAGGACCC 
AAATATCTCC CAGTTAGGTG GTGGCATTCT TCACGTTCTC TTCAAGATGA CTCCATAGTT 
GAAAAGTCCC TGAAGTCCTT AAAGGACAAA AACAAGAAGT TGGAAGAAGG GGGTCCTGTA 
TATAGTCCAA CAGAAGTGGA AGTTGTGAAA AAATCTATTG GACAGAGGAT TGTGGATGAG 
CTGAAGCACT ATTACCATGG TTTTCGATTA CTCTGGATTG ACACAAAAAT AGCTGCAAGG 
ATGCTTTGGC GAATACTACA TGGAAATACT CTGTCACGTC GAGAACGGAG ACAGTTTCTT 
CGAATATGTG CTGATCTCTT CCGCTTGGTT CCTTTCCTCG TTTTTCTCGT TGTCCCATTT 
ATGGAGTTTC TGCTTCCAGT AGCTCTGAAG TTGTTCCCTA ATATGCTTCC GTCTACATTT 
GAGACAAAGT CTAAAAAGGA GGAAAGATTG AAGAAACAAC TGAGAGTAAA GCTTGAATTA 
GCTAAATTCC TTCAAGATAC AATTGAGGAG ATGGCCTTAA AAAATAAGGC AGCCAAAGGA 
AATGTTACAA AAGATTTTTC AACGTTCTTT CAAAAGATCA GGGAGACTGG TGAAAGACCC 
AGTAATGAGG AGATCCTGAG GTTCTCAAAA CTATTTGAAG ATGAATTGAC TCTGGACAAT 
CTAACCAGGC CTCAGTTGGT GGCACTTTGT AAATTGTTGG AACTTCAGTC AATTGGGACG 
AATAACTTTC TCCGCTTCCA GCTGACTATG AGGTTGAGAA CTATAAAAGC AGATGACAAA 
ATGATTGCTG AAGAAGGAGT TGACAGCCTG ACTGTTAAAG AACTGCAGGC AGCCTGTCGT 
GCACGAGGAA TGAGAGCCCT TGGTGTGACA GAAGAGCGCC TCAGGGAACA GCTTAAACAG 
TGGCTAGATC TACACTTGAA CCAGGAAATC CCTACTTCAT TGCTCATTTT ATCCAGAGCC 
ATGTATCTTC CAGACACGCT TTCTCCAGCT GATCAGCTCA AAACAACGCT TCAGACACTA 
CCAGAGAGTG TTGCCAAAGA GGCTCAAGTC AAAGTGGCGG AAGTTGAAGG TGAAAAAGTA 
GATAACAAAG CACGGCTGGA GGCAACGCTT CAGGAAGAAG CAGCCATCAG AAAAGAAAAT 
GAGGAAAAAG AGATGGAAAG AATAACTGAA GCTGCAGAGA AAGCCAAAGA AACTCTTCAG 
GTTGCAGCCA TGAAATTGGA ATCAGCAGTA GATCTTGAGC CAGCTGTTCT GCAAGTTAAA 
GAAAGTCAGG TGGCTATGGA CAGCAAACAA GAGCTGGCAA AAGCAGATAT GGAAACGTTG 
AAAGATACAG CACCCGTACT GGAAGGCATT AAGGGTGAGG AAATAACTAA GGAGGAGATT 
GACATGCTGA GTGATGCTTG TAACAAGCTG CAGGAACAGA AGAAATCACT AACAAAGGAA 
AAGGAGGAGC TTGAGGAACT GAAGGGTGAT ATCCAGGAAT ATAATGAAGA TTTGCAAGAA 
ATAAAGGAGC TTTCTAAAGC TGGCCAGGAA GAAGTAGTAG AAGAATCAAA GGCGAGCAAG 
CGACTGACCA AAAGAGTGAA CCGGATGATT GGTCAGATAG ACAAAATTAT TAATGAGTTA 
GAGACGAATC AAAAGACGGT GGATGTAAAG CTTGATAGAG GTGACAGTCC TCCTGCTGGA 
TTGTGCTTCC TCTTTAGGGA GAATCTAATC AGTATTGCTG AGCTAATTAA TGCAATGAAA 
CAAATCCAGA AGATTCCAGA GGAGAAGCTA ACGAGGATTG CAGAGGCATT AGATGAAAAC 
AAAGATGGCA AAATTGATAT AGATAATGTT GTTAAGGTAG TAGAATTGAT AGACAAAGAA 
GATGTTGATA TTGGCACCAG CCAGGTCGCT GAGATTATGG CACTGCTTCA AAAAGAAGAA 
AAACTGGAAG AAAAGGAGAA AGCAAAGGAA AAGCAAGATA AAGAAGCTGC AGAAGTAAAG 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LETM1 ( XM_015285900.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015141386.1 Gallus gallus leucine zipper-EF-hand containing transmembrane protein 1 (LETM1), transcript variant X1, mRNA.

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CloneID	OGa35807
Clone ID Related Accession (Same CDS sequence)	XM_015285900.2 , XM_015285900.1
Accession Version	XM_015285900.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	LETM1 and EF-hand domain-containing protein 1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455864.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCA TGCTGCTACA GAGCTGCGGC CGGCGGGCCT GCCGCCTGCC CCGCGCGCTG 
CGCCGCCAGG CCCCGCTGGG TAACTTGGGA GATCGCGCTT GTCTTAGTTG TACATCCCTG 
AGGTTGGCAA ACAAAATGAC TGTACACTTT AAATATTGCA CTAGTGTTGC TCCTGTTTAC 
GCATACTCCA AAAAAGATCA CTACTGTTGT TGGACTAAAG GATTGGAACG AACGTACTTT 
ACTCTTATGA GCTCTTCTGG CTCATGGACG CCCCTGGCAG CCATGGGTGT TTTAGGACCC 
AAATATCTCC CAGTTAGGTG GTGGCATTCT TCACGTTCTC TTCAAGATGA CTCCATAGTT 
GAAAAGTCCC TGAAGTCCTT AAAGGACAAA AACAAGAAGT TGGAAGAAGG GGGTCCTGTA 
TATAGTCCAA CAGAAGTGGA AGTTGTGAAA AAATCTATTG GACAGAGGAT TGTGGATGAG 
CTGAAGCACT ATTACCATGG TTTTCGATTA CTCTGGATTG ACACAAAAAT AGCTGCAAGG 
ATGCTTTGGC GAATACTACA TGGAAATACT CTGTCACGTC GAGAACGGAG ACAGTTTCTT 
CGAATATGTG CTGATCTCTT CCGCTTGGTT CCTTTCCTCG TTTTTCTCGT TGTCCCATTT 
ATGGAGTTTC TGCTTCCAGT AGCTCTGAAG TTGTTCCCTA ATATGCTTCC GTCTACATTT 
GAGACAAAGT CTAAAAAGGA GGAAAGATTG AAGAAACAAC TGAGAGTAAA GCTTGAATTA 
GCTAAATTCC TTCAAGATAC AATTGAGGAG ATGGCCTTAA AAAATAAGGC AGCCAAAGGA 
AATGTTACAA AAGATTTTTC AACGTTCTTT CAAAAGATCA GGGAGACTGG TGAAAGACCC 
AGTAATGAGG AGATCCTGAG GTTCTCAAAA CTATTTGAAG ATGAATTGAC TCTGGACAAT 
CTAACCAGGC CTCAGTTGGT GGCACTTTGT AAATTGTTGG AACTTCAGTC AATTGGGACG 
AATAACTTTC TCCGCTTCCA GCTGACTATG AGGTTGAGAA CTATAAAAGC AGATGACAAA 
ATGATTGCTG AAGAAGGAGT TGACAGCCTG ACTGTTAAAG AACTGCAGGC AGCCTGTCGT 
GCACGAGGAA TGAGAGCCCT TGGTGTGACA GAAGAGCGCC TCAGGGAACA GCTTAAACAG 
TGGCTAGATC TACACTTGAA CCAGGAAATC CCTACTTCAT TGCTCATTTT ATCCAGAGCC 
ATGTATCTTC CAGACACGCT TTCTCCAGCT GATCAGCTCA AAACAACGCT TCAGACACTA 
CCAGAGAGTG TTGCCAAAGA GGCTCAAGTC AAAGTGGCGG AAGTTGAAGG TGAAAAAGTA 
GATAACAAAG CACGGCTGGA GGCAACGCTT CAGGAAGAAG CAGCCATCAG AAAAGAAAAT 
GAGGAAAAAG AGATGGAAAG AATAACTGAA GCTGCAGAGA AAGCCAAAGA AACTCTTCAG 
GTTGCAGCCA TGAAATTGGA ATCAGCAGTA GATCTTGAGC CAGCTGTTCT GCAAGTTAAA 
GAAAGTCAGG TGGCTATGGA CAGCAAACAA GAGCTGGCAA AAGCAGATAT GGAAACGTTG 
AAAGATACAG CACCCGTACT GGAAGGCATT AAGGGTGAGG AAATAACTAA GGAGGAGATT 
GACATGCTGA GTGATGCTTG TAACAAGCTG CAGGAACAGA AGAAATCACT AACAAAGGAA 
AAGGAGGAGC TTGAGGAACT GAAGGGTGAT ATCCAGGAAT ATAATGAAGA TTTGCAAGAA 
ATAAAGGAGC TTTCTAAAGC TGGCCAGGAA GAAGTAGTAG AAGAATCAAA GGCGAGCAAG 
CGACTGACCA AAAGAGTGAA CCGGATGATT GGTCAGATAG ACAAAATTAT TAATGAGTTA 
GAGACGAATC AAAAGACGGT GGATGTAAAG CTTGATAGAG GTGACAGTCC TCCTGCTGGA 
TTGTGCTTCC TCTTTAGGGA GAATCTAATC AGTATTGCTG AGCTAATTAA TGCAATGAAA 
CAAATCCAGA AGATTCCAGA GGAGAAGCTA ACGAGGATTG CAGAGGCATT AGATGAAAAC 
AAAGATGGCA AAATTGATAT AGATAATGTT GTTAAGGTAG TAGAATTGAT AGACAAAGAA 
GATGTTGATA TTGGCACCAG CCAGGTCGCT GAGATTATGG CACTGCTTCA AAAAGAAGAA 
AAACTGGAAG AAAAGGAGAA AGCAAAGGAA AAGCAAGATA AAGAAGCTGC AGAAGTAAAG 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015141386.1
CDS	135..2420
Translation	MASMLLQSCGRRACRLPRALRRQAPLGNLGDRACLSCTSLRLANKMTVHFKYCTSVAPVYAYSKKDHYCCWTKGLERTYFTLMSSSGSWTPLAAMGVLGPKYLPVRWWHSSRSLQDDSIVEKSLKSLKDKNKKLEEGGPVYSPTEVEVVKKSIGQRIVDELKHYYHGFRLLWIDTKIAARMLWRILHGNTLSRRERRQFLRICADLFRLVPFLVFLVVPFMEFLLPVALKLFPNMLPSTFETKSKKEERLKKQLRVKLELAKFLQDTIEEMALKNKAAKGNVTKDFSTFFQKIRETGERPSNEEILRFSKLFEDELTLDNLTRPQLVALCKLLELQSIGTNNFLRFQLTMRLRTIKADDKMIAEEGVDSLTVKELQAACRARGMRALGVTEERLREQLKQWLDLHLNQEIPTSLLILSRAMYLPDTLSPADQLKTTLQTLPESVAKEAQVKVAEVEGEKVDNKARLEATLQEEAAIRKENEEKEMERITEAAEKAKETLQVAAMKLESAVDLEPAVLQVKESQVAMDSKQELAKADMETLKDTAPVLEGIKGEEITKEEIDMLSDACNKLQEQKKSLTKEKEELEELKGDIQEYNEDLQEIKELSKAGQEEVVEESKASKRLTKRVNRMIGQIDKIINELETNQKTVDVKLDRGDSPPAGLCFLFRENLISIAELINAMKQIQKIPEEKLTRIAEALDENKDGKIDIDNVVKVVELIDKEDVDIGTSQVAEIMALLQKEEKLEEKEKAKEKQDKEAAEVKN

Target ORF information:

RefSeq Version	XM_015285900.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus leucine zipper-EF-hand containing transmembrane protein 1 (LETM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285900.1

ORF Insert Sequence:

ATGGCGTCCA TGCTGCTACA GAGCTGCGGC CGGCGGGCCT GCCGCCTGCC CCGCGCGCTG 
CGCCGCCAGG CCCCGCTGGG TAACTTGGGA GATCGCGCTT GTCTTAGTTG TACATCCCTG 
AGGTTGGCAA ACAAAATGAC TGTACACTTT AAATATTGCA CTAGTGTTGC TCCTGTTTAC 
GCATACTCCA AAAAAGATCA CTACTGTTGT TGGACTAAAG GATTGGAACG AACGTACTTT 
ACTCTTATGA GCTCTTCTGG CTCATGGACG CCCCTGGCAG CCATGGGTGT TTTAGGACCC 
AAATATCTCC CAGTTAGGTG GTGGCATTCT TCACGTTCTC TTCAAGATGA CTCCATAGTT 
GAAAAGTCCC TGAAGTCCTT AAAGGACAAA AACAAGAAGT TGGAAGAAGG GGGTCCTGTA 
TATAGTCCAA CAGAAGTGGA AGTTGTGAAA AAATCTATTG GACAGAGGAT TGTGGATGAG 
CTGAAGCACT ATTACCATGG TTTTCGATTA CTCTGGATTG ACACAAAAAT AGCTGCAAGG 
ATGCTTTGGC GAATACTACA TGGAAATACT CTGTCACGTC GAGAACGGAG ACAGTTTCTT 
CGAATATGTG CTGATCTCTT CCGCTTGGTT CCTTTCCTCG TTTTTCTCGT TGTCCCATTT 
ATGGAGTTTC TGCTTCCAGT AGCTCTGAAG TTGTTCCCTA ATATGCTTCC GTCTACATTT 
GAGACAAAGT CTAAAAAGGA GGAAAGATTG AAGAAACAAC TGAGAGTAAA GCTTGAATTA 
GCTAAATTCC TTCAAGATAC AATTGAGGAG ATGGCCTTAA AAAATAAGGC AGCCAAAGGA 
AATGTTACAA AAGATTTTTC AACGTTCTTT CAAAAGATCA GGGAGACTGG TGAAAGACCC 
AGTAATGAGG AGATCCTGAG GTTCTCAAAA CTATTTGAAG ATGAATTGAC TCTGGACAAT 
CTAACCAGGC CTCAGTTGGT GGCACTTTGT AAATTGTTGG AACTTCAGTC AATTGGGACG 
AATAACTTTC TCCGCTTCCA GCTGACTATG AGGTTGAGAA CTATAAAAGC AGATGACAAA 
ATGATTGCTG AAGAAGGAGT TGACAGCCTG ACTGTTAAAG AACTGCAGGC AGCCTGTCGT 
GCACGAGGAA TGAGAGCCCT TGGTGTGACA GAAGAGCGCC TCAGGGAACA GCTTAAACAG 
TGGCTAGATC TACACTTGAA CCAGGAAATC CCTACTTCAT TGCTCATTTT ATCCAGAGCC 
ATGTATCTTC CAGACACGCT TTCTCCAGCT GATCAGCTCA AAACAACGCT TCAGACACTA 
CCAGAGAGTG TTGCCAAAGA GGCTCAAGTC AAAGTGGCGG AAGTTGAAGG TGAAAAAGTA 
GATAACAAAG CACGGCTGGA GGCAACGCTT CAGGAAGAAG CAGCCATCAG AAAAGAAAAT 
GAGGAAAAAG AGATGGAAAG AATAACTGAA GCTGCAGAGA AAGCCAAAGA AACTCTTCAG 
GTTGCAGCCA TGAAATTGGA ATCAGCAGTA GATCTTGAGC CAGCTGTTCT GCAAGTTAAA 
GAAAGTCAGG TGGCTATGGA CAGCAAACAA GAGCTGGCAA AAGCAGATAT GGAAACGTTG 
AAAGATACAG CACCCGTACT GGAAGGCATT AAGGGTGAGG AAATAACTAA GGAGGAGATT 
GACATGCTGA GTGATGCTTG TAACAAGCTG CAGGAACAGA AGAAATCACT AACAAAGGAA 
AAGGAGGAGC TTGAGGAACT GAAGGGTGAT ATCCAGGAAT ATAATGAAGA TTTGCAAGAA 
ATAAAGGAGC TTTCTAAAGC TGGCCAGGAA GAAGTAGTAG AAGAATCAAA GGCGAGCAAG 
CGACTGACCA AAAGAGTGAA CCGGATGATT GGTCAGATAG ACAAAATTAT TAATGAGTTA 
GAGACGAATC AAAAGACGGT GGATGTAAAG CTTGATAGAG GTGACAGTCC TCCTGCTGGA 
TTGTGCTTCC TCTTTAGGGA GAATCTAATC AGTATTGCTG AGCTAATTAA TGCAATGAAA 
CAAATCCAGA AGATTCCAGA GGAGAAGCTA ACGAGGATTG CAGAGGCATT AGATGAAAAC 
AAAGATGGCA AAATTGATAT AGATAATGTT GTTAAGGTAG TAGAATTGAT AGACAAAGAA 
GATGTTGATA TTGGCACCAG CCAGGTCGCT GAGATTATGG CACTGCTTCA AAAAGAAGAA 
AAACTGGAAG AAAAGGAGAA AGCAAAGGAA AAGCAAGATA AAGAAGCTGC AGAAGTAAAG 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Genome biology6(1)R6(2005)
Caldwell RB,Kierzek AM,Arakawa H,Bezzubov Y,Zaim J,Fiedler P,Kutter S,Blagodatski A,Kostovska D,Koter M,Plachy J,Carninci P,Hayashizaki Y,Buerstedde JM

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LETM1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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