FRAS1 cDNA ORF clone, Gallus gallus(chicken)

The following FRAS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FRAS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa07890 XM_025150291.1
Latest version!
Gallus gallus Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa07890 XM_420470.6
Latest version!
Gallus gallus Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa150560 XM_025150292.1
Latest version!
Gallus gallus Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa35354 XM_015276618.2
Latest version!
Gallus gallus Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
OGa07890 XM_420470.5 Gallus gallus Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa35354 XM_015276618.1 Gallus gallus Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa07890
    Clone ID Related Accession (Same CDS sequence) XM_420470.6 , XM_025150291.1 , XM_420470.5
    Accession Version XM_420470.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11970bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product extracellular matrix protein FRAS1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_420470.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
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    7621
    7681
    7741
    7801
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    7921
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    8041
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    8161
    8221
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    9001
    9061
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    9241
    9301
    9361
    9421
    9481
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    9601
    9661
    9721
    9781
    9841
    9901
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    10021
    10081
    10141
    10201
    10261
    10321
    10381
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    10501
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    11101
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    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    ATGGGCGCCT GGCTGCCGCT GTGCCTGCTC AGCGCCGCGC TGGCCGCCGC CGCCGCCGCC 
    GCCTCAGGTG CCTGTGTCTA TCAAGGCTTG TTGTTTGAGA ATAACACAAT TTGGAAGCCA
    GATTCTTGCC AAGACTGCAG TTGCCAAAGC AATGTTGTCA CCTGTGAACC TGCTGTCTGC
    GAACATCCTC AGTGTGACTT TGAGAAGGGA GAAGTGCTCC AAATAGCCCC AAACAAATGT
    TGCCCTAAAT GTGCCTCACA AACTGAAGGA TATTGCCAGC ATGAAGGACA GATCCACAGT
    CATGGCACGC AGTGGGCCAG CTCTGGATGT GTTCTGTGCT CCTGTGCCCA TGGGAAGGTG
    AGCTGCACGC CCATAGCCTG CCCAGTCCTC ACCTGTGAAA GGGATGAGCT GCCATACACT
    GCCCAGGGAG CCTGTTGTCC CACATGCGTG GGCCTTGGTG AGCCCTGCTA CTTTGATGGC
    CGTTCGTTCC GGGATGGTGA GGACTGGCGC CTTGGTCGGT GCTCCAAATG TGTCTGCAGG
    GATGGCGTGA CTCAGTGCTT CACAGCTTCC TGTGAGGCTG TTCTGTGCAA ACAGGATGAA
    GTTTTGGCTA TGTCTCCTGG AAAATGCTGT CCAGAATGTG TTCCAAAGTC CTGCTCGGTG
    TCAGGAAAGG TGTACCAGCA CGGAGAACGG TGGAAAAAAA ATGCCTGCAG TGCGTGTGCG
    TGTCATAGAG GGGCAGTCAG ATGTCTGAGA GAAACCTGTG ACTCTGTTAT TTGTGAGAAG
    GGGGATAACA AAGTGCAGCG TTCCGGGAAG TGCTGTGAGG AGTGTGTGTC TTCCAAGGAA
    AACTGCTTGT ATGATGGCAC TATCAGGTAC CACGGTGAGA TGTGGAATAT TACTCGCTGT
    GACTTCTGCA TGTGTGATGA AGGTCAAGTG ACTTGTCACA AAGCAGAGTG CGCCAAAGTA
    GAATGTGCCA AGGGTGAAGA ATTAATTCAC TTAGATGGGA AGTGCTGTCC TGAATGTATT
    TCAAGTAATC GTCATTGTTT TTACAAAGGA CATACCAAAG CTAGCGGAGA GACGTGGAAG
    GAAGGGCTGT GCAGAGAGTG TGAATGCCGC GACTCAGAGG TGGTCTGCTT TCAGCTTTCC
    TGTCCTCCCT GCCCACCGGG CAGCATGGCT GTCAGGGAGA AGGGTGATTG CTGTCCACGC
    TGCCAGCCAG GAAAGTGCCA CCCAGACTGT TTGACCTGCT CGCAGTCCTT TGATCACTGT
    ATTGCCTGCC GTGATGTGAA GAAGCAGCTG CAGAATGGGC GCTGTGTGGA GAGCTGTGGG
    TCAGGCTTCT ATCAGGATGG GGGGACTTGC TTAGCCTGCA ATGAAACATG CTCAGCCTGT
    ACGAATGGTT TTGAGTGTTC CTCATGCCAG ACGTCCCTGC TGATGAAGAA TGGGCAGTGT
    GTGACTTCAT GTGGGAAGGG ATTCTTTCAG GATCAACTCA TATGTGCAGT TTGTCATGAG
    TCCTGTTCCT CGTGTTGGGG ACCTGCTGAC AACAGTTGTC TCTCCTGCAA AGACACGTCA
    CATGTGCTAA AAGCAGGGCT CTGCGTGGCC AGCTGTGGAA AGGGATTTTA TGAGAAGGAT
    GGAATCTGCA GTGTTTGTGA CCCGTCCTGT GAAACGTGCA ATTCAGCCCA GCCCAGTTGT
    TTAACCTGTG CTTCAGATAA GGTGTTACAC AACGGGAACT GCATTTCAGA GTGCCCAGGC
    GGATATTTCC CAGGCAGCAA TGGAAGATGC AGGGCTTGTC ATGATTCGTG CTCCACCTGT
    GAGGGACCTC TGGCCACTCA CTGTACCTCC TGTTCCCTTC CTCTGGCTTT GCACCAGGGC
    CAGTGCCTGC AGGGCTGTGG AGAGGGCTTT TATCAGGATC ACAGCATTTG CAGGGGTTGC
    CACCCATCCT GCCGTAACTG CATTGGCCCA GGGGCCTCTC ATTGCCTGCA GTGCTGGGCA
    CCAGAGGATG TGCTGCAGCC CCATCAACCT GTGGAAGGAG CTGTGCATGG CTTTTGCCTC
    TCACACTGCC AGGCCCAGTT CTACCTGGAT GTCACAGGAG TCTGCAAACA GTGCCACTCC
    TCCTGTGCAG GCTGCATAGG GAACACTTCC CAGGACTGTA CGGCCTGTCC GTCTTCGCAC
    ATGCTGCTCG AGGGCAGATG CCTCTCCGAG TGTCCAGAGG GGTTGTTCAG CCAGCAGGAT
    CACTGCTCCT CCTGCCATCC GTCATGCAAG ACATGCCATG GCCCTTCTGA TTTGGAGTGC
    TTAACCTGCC ATCCCCATGC AACTCTGGCT GCTGGAAGGT GCAGGACTAG CTGCAAGGAA
    GAGCAGTATC TCAACTTGGT TGGATATTGC GTGGACTGCC ATCCTTTGTG CCATCAGTGT
    GTGGCCAACC TGTGGGACAC TGGCAGCCTC TGTCTCAAGT GCCAGAATGC CCGTCACTTG
    CTCCTTGGGG ATCACTGCGT TCCTGACTGT CCAGCAGGAT ACTACACAGA GAGCGGCGCC
    TGCAAACGAT GTCACCCCTC ATGTAAAACC TGCAGAGGTA GGGGTCCTCT TTCCTGCTCC
    TCCTGCAACA CCAGCCTGGT TCTGTCCCAC ACCAGCATGT GCGTTCCAGT CTGTTCTCCG
    GGATACTACA AGGATCAGAG CCAGACCTGC AAACCTTGCA ACAGCCAGTG CCTGAACTGT
    GAATCAGCTA CAGGCTGTAT GTCTTGCAGA GATCCAGCTA AGGTCCTGTT ATTTGGAGAA
    TGCCAGTATG AGAGCTGTGC TCAGCAGTAT TATCTGGATT TTTCAACCAA GACTTGCAAA
    GAGTGTGACT GGAGCTGCAA TGCTTGTAAA GGCCCTCTGA GGACCGATTG CCTGCAGTGT
    ATGGAGGGTA GTGTACTCCA CAAAGGAGCT TGTGTGGAAC AGTGCCCCAC AGCTTTCTAC
    AAGGAGTCAG ACATGTGCCA GAGGTGTGAT CAACATTGCC TTCAGTGTCA CCAAGCAAAT
    GAGTGCAGCC TTTGTGAAGC CCCATTTTTC CTCTTGGACA CTCAGTGCGT TTATAAGTGT
    GGGAAGGGAT ATTATGCAGA CCACGCACAG CGAAAATGCA TAGCTTGTCC TCACGGGTGC
    TTGGAGTGTG ACAGTGACGG CCTTTGCCAC CTCTGTGATG GCAACACCTT TCTGAAAAAC
    AAGATTTGCG TTTCTGCCTG TGGACAGGGA TTCTATGGCA ATAAGTGGAC CAGAGAGTGT
    GAAGTCAGCA GGCATCCTTC AAGCTTCCGT GTAAACAGTA CCCTCACAGT AGGGATTGGT
    GGGGTAAAGC CTCTAGACCT CTCCTTACTG GATGTGCGTG ATCTGGATGG TGACACAGGA
    CAGCTCTTGT TCCATATTGA CAGCGCTCCC TCCAATGGCA GGCTAGTGAT GGTGGTGAAT
    GGGAAAGAAG CGCAGCTGAG CAAAGGTGAC CACTTCAGCT TCAAGGATGT GAAGGAGAAT
    AGAGTCCGCT TTGTGCACAG CAGAGAGAAA TCCAGGAAAG GACAGCTTTC CTTGAGAGTC
    AGCGATCAAC AGTTCTCCCA TCCTGAGGTG ATCAACATTC AAGCATTTTC AACACAGGCC
    CCCTATCTGC TGAGAAATGA GGCCCTCCTT GTCCGCAGAG GGGAAATTGG AACTATAACA
    GACCTGTTGC TTGATGTGAG AGATGGTGAC AATCCTCAGG ATGTCATGTT GATGGTTTTA
    GAGCCTCCAC GTCATGGTCA GCTGATAAAA CCTCCCAGGG ACTCAGCTTC TTCAGTCTAC
    ATGTTCCACC TCGATGACCT TGCTGGTGGA CTGCTACGTT ATGCTCACGA TGGCTCTGAC
    ACCCAGGATG ATGCAATTCT TCTGCAAGTG AACGATGGAT ACCACTTCCA GAACATCCTG
    TTCCACATTA AGATCGCTCC CAAGAGTTAC AGAAGCCCTT GGCTGGTGAC AAATTCCCTG
    GTCTGGGTGC CCCAGGGAGG CATGCTGCAG ATCTCCAAGA CAATTCTGCA CGCTGAGGTA
    CCTGGAGCCC GTGACTCTGA GATAACCTAC AACATCATCA AGGACCAGCC AAGACATGGT
    GAAGTGGTGC TTCTGGTCCC AATGCCGGCA GATGGCCCAG CAGAGTCATG GCAGCTTTTG
    CCTGATGGAA GAGCGGCCTC ACCTACAACC TCTTTTACCC AGCAGGACAT CAATAATGGC
    ATCGTGTGGT ACAGGCACTC TGGAACTCAA GTAGAGAGTG ACTCCTTCCA GTTCCAGGTG
    TCCAGCTCTG CCAGTCCACA AGCCAGCTTG GAAAGCCACG TGTTCAACGT TGCTGTGCTC
    CCACAGACAC CCAGAGCACC TCAGCTTTCC CTTGGCTCCT CTTTGCACAT GGCTGTGCTG
    GAGGACCGCG TGACAGTGAT TGAGCCCCAT CACCTCTCCT TTGTAGACCC AGAGATTCCC
    AGTGAGAAAA TCCTGTTCAA CGTGACTGTC CCTCTGCTTC CTGGCCAAGG TATTGTGGAG
    CACAGAGACA GGCCTCACTC GCCAGTCAGG TATTTCACTC AAGCAGATAT AAACCATGGC
    AAGATTGCTT ATCGTCCACC AACTGCAGCT CCACACTTGA GAGAGATCAT TGCATTCTCA
    TTTGCAGGTC TCCCAGAGTC AGTGAACTTC CGATTCACAG TGTCTGGTGG AGAACACACA
    ACCCCGGAGA TGGCGTTTGT CATTCACTTG CTCTCTGCAG AGCAGCAGCC TCCAGTCTTC
    CAGATCACAG CTCCTTTCCT GGAGGTCAGC CAGGGGGGAA AGGCCACCAT CGGTGTCCAG
    TTGGCTGTGA GTGACACAGA TACAGCTCCT GAGGGCCTCT TCTTTGAGCT GGTGAAACCT
    CCCCATCATG GCATCGTGCT CAAGCATGAT GCAGGTGTGC AGGAGCACAT GAGAGCAGGT
    GACACCTTCA CCTATGAGGA CGTCACTCGA AATGCTCTGC AGTACGTACA TGATGGCTCA
    TCGGCAGCAG AAGACAGCAT GGAAATCTCT GTCACCGATG GCGTCACCAC AGTGACCACA
    GTGCTGAGAG TGGAGGTGTC CCTACTGGAT AACAACGGCC CACAGCTGGC CCCAGGCTGC
    CTGTTGGCAA TCACTGTGGC TAGTAAAAGC TCTGCAACTC TCTCTCGACT GCACCTCGCT
    TACATTGACA ACAATTCTCC TGACTCAGAG ATAAGAATCC AGTTAATATC TCTGCCCACG
    TACGGCACCC TCTTACGGAC GTCTGGCTCT CATGCTGAAG AACTTTCTAG GGTTTATAAT
    TTCACCATGG AAGACATCAA CAACCAGAGG ATCAGTTACT CCACTGTGTT TGAGACAAGC
    AATCAGCCAG TTACGGATGC CTTTCACTTC ACTGTTTTTG ATGCTGATAA TAACCGGCTT
    GACAGACAGA TGTTTACCAT CACCATCACG TCTGCCCAGA CAGCACCATC ACTCATTGTG
    TTTGCTGACC ATATCGCTGT GGATGAGGGG GGCAGGGTCC CACTTCTGGC TCATCACCAC
    TTAACTGCTG ATTATGATAC TGCTGCCAAG GAAGACCTGA GGGTCACGGT TATCACTCTA
    CCCGTGTATG GCTACATTGA AAACACTAAG ACAGGTGAGA CTTTTGGACC ACAGAGTGCT
    GTGACCACAG ATGCATCCTT CTCCATTCAA GATGTTCTAG AGAACAACAT TTACTACTTT
    CAGAGCGTCC ATGAAAGCAT CGAGCCAACA AGTGATGTTT TCAGCTTTTA CGTGAGCGAT
    GGCTTCAGCC AGTCTGAGGT GCACAGCATC AACATCACCA TTCAGCGACG GAATGATGAG
    CCCCCAAAGA TGGTGTTGGA GCCTGTCAGA GTGCATGAGA GCTCAGTGGT TGTTACCAGT
    GCCTCACTCA ACCTGCAGGA CCTGGACACC CAGAACAGTG AGCTTGTCAT CATGGTTACC
    AAAACTCCTG AGCACGGTCA CCTTCGCAGG AGGCAGACTG CTGTGGAGCC CCCAGAGCGT
    GGACACGTGC TGTCTCTGGG CTCATCCTTC ACCTACCAGG ACATTTTGGA TGAGCTGATT
    GTTTATTCTC AAAGCGGTGC AGCAGCACAG ACGGATGAGT TTGGCTTCTC CATCACAGAT
    GGTCTCTACA TGCAGACAGG GAAGCTGGAG TTCTCCATGG AGCTTCCAAA GAAGCAGCTG
    CCCACATTGA CTGCCAGCAG GGTCCTGCAG CTCCCCACTG GCTCTGCAGC ACACATCACA
    GATCAACACT TGAAAGCAGC AGCTGTTTAT CCAGATGACA CAATGATCAG ATTTGTCATG
    AAGAGGGATC CCAGCGTGGG CCATTTGCAG TTGACTGATA TTGATAATCC AGTCGAGATC
    TCTGTCAGAG GGCCTGTCAA AAGTTTTACC CAGGCTGATA TAAATAAAGG GCAAGTGGTT
    TATAGTCACA AGAAGGGGGA TCCTGGTGGA AATTTTGCCT TCACCTTTGA TGTGATCGAT
    GCAGATGGCA GCAGACTGAT GGACCAGTTT TTTCATATCA CTGTATTAGA AGACAGGTTC
    CCCCCCTCCA TCGTCGCTAA CACAGGGCTG GTCTTAGATG AGAACTCTGC AAAGAAGATC
    ACAACACTGC AGCTCTCTGC CACTGACCGG GACAGTGAGC CGACCCAGCT CCAGTACAAG
    CTCACCAAGC AGCCACAGCT GGGGCACCTG GAGCATGTGG CCTCCCCAGG GACAAAAATC
    AGTTCTTTCA GCCAGGCAGA CTTGGCCTCT CGCAGCATCC AATACGTTCA CACTAGCGAC
    ACTGAAAAGC ATACGGATGC CTTCACCTTC TCTGTGTCTG ATGGAACAAA TGAGGTCAGC
    CAGACATTTG ACATCACCAT AAACCCTGTG GATGATTCCT TACCTGTAGT GCAAAGCTTT
    GGGATGAGAG TTCAGGAAGG TGTGAGAAAA ACCATCACAG AGTTTGAGCT TAAAGCAACA
    GATGCTGACA CAAAGGCTGA GGCAATTACA TTCACAGTCG TGCAGCCCCC TCGACATGGC
    CTGATTGAAC ACACCAGCAA TGGGCAGCAT TACCACCAAG CCACCACCTT CACCATGGAT
    GACATTTACC AGAACCGCAT CAGCTACAGT CACGATGGAA GCAACTCACT GAAGGATCGC
    TTTGCTTTCA CTGTGTCTGA TGGGACCAAT CCCTTCTTCA TTATGGAGGA TGGGGGGAAA
    AAGGTTGTGA CAGCAGCACC ACAGCGCTTC AGAATGGACA TCCTCCCTGT GGATGATGGG
    ACACCCAGGG TGGTCACCAA CCAGGGTTTG CAATGGCTGG AGTATGTGGA TGGCAAGGCA
    ACCAATCTAA TCACCAAGAA GGAATTACTG ACAACAGATC CTGACACAGA TGACAAACAG
    CTGATCTATG AGATAACAGA TGGTCCCAGG AATGGTTATG TGGAGAACAA GCTGAAACCA
    GGAACAACTG TGACCATGTT TTCTCAGGAG GATGTGAATC AGGGCCTCAT TCGGTATGTG
    ATGCATGAGG AGAAGGTTCA GGAGACTATG GACAGTTTTC AGTTCCTCGT GAAGGACAGC
    AAACCCAATG TGGTCCGTGG CAACGTCTTC CACATCCAGT GGTCTCTCCT CAGCTTTACA
    TACAGCAGCT ACAACGTCAG TGAGAGCGCT GGCTCTGTGA GCGTCACCGT GCGGCGGATT
    GGTAACCTTA ACCAGTATGC CATTGTCTTG TGCCGCACGG AGCAGGGAAC AGCCACTTCC
    AGCTCTGGTT CACAGTCTGA AGAGCAGGAC TATGTGGAGT ACGCAGGGCA GGTGCAGTTT
    GAGGAGCGGG AAGACACCAA GGCCTGCACC ATCGTCATTA ACGATGACGA CATCTTTGAG
    AGCACCGAGA GCTTCACTGT GGAGCTCAGC ATGCCAGCCT ATGCTCTGCT GGGGAATGTC
    ACCCATGCCA CAGTCACCAT CACAGACGCT GAGGATGAGC CCATGCTGCA GTTCGACAGG
    AAGACGTACC ACGTCAGTGA GAGCGCTGGG TTTCTCTCTG CGCCTGTTGA AAGGAAAGGA
    GATGCTAGCA GCACTGTGTC TGCTGTCTGC TACACTGTCC CCAAATCAGC CAAGGGAAGC
    AGCCTTTATA TGCTGGAGTC TGGCTCCGAC TTCAAGTCCC GAGGGATGAC AGATGACAGC
    CGCATAGTTT TTGGACCAGG TGTTACCATG TCGACATGTG ATGTCATGCT TATTGATGAC
    AGTGAATATG AAGAAGAAGA GGAGTTTGAG ATAGTATTGG CTGATGCTTC GGAAAATGCA
    CGCATTGGCA ACCGGGCTTC AGCCAGCGTC TTCATCGGTG GTCCCAATGA TGCCTCGACA
    GTGTTCCTGG GAAATGCCAC GTTCACCGTC AGTGAGGCTG CAGGAAACAT TGAAATTCCA
    GTCCTCCGTC ACGGACCTGA TCTTTCAACC CCCACCTCAG TGTGGTGTGC TACTCGGACA
    TCAGATCCTC CATCTGCCAG CCCAGGAGTT GATTATGTCC CCAGCTCCAG AAAAATAGAG
    TTCAGGCCAG GCAAGACAGA AGAGTTCTGC ACTCTGACAA TCCTGGATGA TGCTCAGTAC
    CCAGTGATAG AAGGGCTGGA GACATTTGTT GTTTATCTCA GCTCACCCCA TGGAGCTGAG
    CTGATAAAGC CCTTCCAAGC CATTGTGGCC ATTAATGACA TCATCCAAGA TGTACCAACC
    ATGCAGTTCA CCAAGGATGT GTTCACAGTG AAGGAAAAGG AGGGTGCACT ACACATCCCC
    ATTTTCCGCA CTGGCGACCT GAGCTATGAG TCCTCCGTGA GGTGCTACAC CCAGAGCCAG
    ACAGCAGAAG TCATGGAGGA CTTTGCAGAG AGGAGAAATG CAGACGAGTC CCGCATCACT
    TTCTTGAAAG GGGAGAAGGT GAAAAACTGC AGTGTTTACG TCAGTGATGA CTCTGCCTTT
    GAACCAGAGG AGCAGTTTCA TGTCCATCTT GGTAGTCCGC TTGGAAACCA TTGGAGTGGA
    GCTAGGATTG GGAAGATAGA CATGGCAACT GTGACCATCA CTAATGATGA AGACGCACCC
    ACCATTGAGT TTGAAGAAGC TGCGTACCAG GTACGGGAGC CACCTGGACC AGAGGGTGTC
    GCTGCTTTAA ATATCAAAGT GGTCCGGAGA GGGGATCAGA ACAGGACCTC AAAAGTGCGC
    TGTAGCACTC GTGATGGCTC AGCTCAGGCC GGAGTGGATT ACTACCCCAA GAGTCGAGTG
    CTCAAATTCA GCCCAGGTGT GGACCACATC ATGTTTAAGG TGGAGATTAT GTCCAACGAA
    GATCGGGAGT GGCACGAGTC CTTTTCTCTG GTGCTGGGTC CAGATGACCC AGTGGAAGCT
    GTTCTTGGAG ACACCAGCAC TGCAACAGTG ACTATTTTGG ATCAGGAGGC AGCAGGCAGC
    CTGATTCTGC CAGCACCTCC TGTGGTCGTC TCCTTGGCTG ATTATGACCG TGTGGAAGAA
    GCGACCAAGC ACGGTGCTAA GAAATCCCCT TCTCCAGGCT ATCCTCTCAT CTGTGTCACT
    CCCTGTGATC CTCACTTCCC CAAGTTCATG GTCATGAAGG AGCGCTGCAG CGAGGCCGGG
    ATCAACCAGT CTTCCATACA GTTCAGCTGG GAAGTAGCAA CCCCCACAGA TGGGAATGGG
    GCCAGGTCGC CTTTTGAAAC CATCACTGAC AACACACCAT TCACCAGCGT CAACCATAAG
    GTGCTGGACA GCATTTATTT CAGCCGGCGG TTCCACGTGC GCTGTGTGGC CAAGGCTGTG
    GACAAGGCTG GCCATGTGGG CACCCCCTTG AGGAGCAACG CAGTTACTAT AGGGACAGAC
    AGTGCCATTT GTCACACTCC TGTGGTCGCG GGGACAGCTC GAGGCTTCCA GGCACAGTCG
    TTTGTTGCCA CTCTGAAGTA TCTGGACGTC AAACACAAGG AGCATCCCAA CAGGATCCAC
    ATCTCAGTGC AGATCCCCCA CCAGGATGGA ATGCTGCCAC TCATCTCCAC CCTGCCCCTG
    CACAATCTGC ATTTCCTGCT CTCTGAGTCT ATCTACAGAC ACCAGCATGT CTGCTCAAAC
    CTGGTCACCA TCAGAGACCT TAAAGGCATC TCAGAGGCAG GATTCCTTGA TGAAGTGACA
    TATGATAGCA TTGTTCTCGG TCCTGGCTAT GACCGCCCTT ACCAGTTCGA CCCCTCCGTG
    AGAGAGCCAA AGACAATCCA GCTGTACAAG CACCTCAATC TGAAGAGCTG CATATGGACC
    TTTGATGCCT ATTACGACAT GACTGAATTA ATTGATGTCT GTGGAGGCTC TGTAACAGCT
    GACTTCCAGG TACGGGACTC TGCTCAGTCC TTCCTAACAG TCCACGTCCC CCTCTATGTG
    TCCTATATTT ATGTGACAGC GCCGAGAGGC TGGGCTTCTC TTGAGCATCA CACAGAGATG
    GAGTTCTCCT TTTTCTACGA CACTGTTCTC TGGAGGACAG GGATTCAGAC GGACAGTGTC
    CTCTCAGCCC GGCTGCAGAT AATCAGAATC TACATCCGTG AGGATGGCCG ACTGGTTATT
    GAGTTCAAGA CCCAGGCCAA GTTCAGAGGG CTCTTTGTAA TGGAGCACCA CAGCTTGCCA
    GATGTGAAGT CGTCTTTGAT GACTCCAGAC CATCTGGGAG GGATCGAATT TGACCTGCAA
    CTGCTGTGGA GTGCTCAGAC TTTTGACTCT CCCTACCAGC TCTGGAGGGC AACCAGCTCT
    TATAACAGGA AGGACTACTC TGGAGAGTAC ACAATCCTCT TAATTCCTTG TACTGTGCAG
    CCTACACAGC CATGGATAGA GCCTGGAGAG AAGCCCCTGC CCTGTACAGC TCATGCTCCC
    GAGCGATTTT TGATCCCAAT CGCCTTCCAG CAGACAAACC GCCCAGTTCC TGTTGTCTAC
    TCCCTAAACA CGGAGTTTCA GCTGTGTAAC AATGAGAAGG TGTTTCTGAT GGATCCCACC
    AAGTCCGAAA TGTCTCTGGC AGAAATGGAT TACAAAGGGG CTTTTTCAAA AGGGCAAATC
    CTGTATGGCC GTGTGCTCTG GAATCCTGAA CAAAACCTAA ACGCTGCTTA CAAACTGCAG
    CTGGAGAAAG TCTACCTGTG CACTGGCAAG GATGGCTACG TGCCCTTCTT TGACCCAACA
    GGAACTGTCT ATAACGAAGG GCCTCAGTAT GGCTGTATTC AACCCAACAA GCACCTGAAA
    CACAGATTTC TCCTCTTGGA CCGAAACCAA CCAGAAGTCA CAGATAAGTA TTTCCACGAT
    GTGCCTTTTG ATGCCCACTT TGCCTCTGAA CTGTCTGAGT TTCACTCAGT AAGCAGCATG
    CCTGGTGTGG ATGGATTTAC TCTCAAGGTG GATGCTCTCT ACAAGGTGGA AGCTGGACAT
    CAGTGGTACC TTCAAGTTAT CTACATAATA GGCCCAGAGA GCATTGCGGG GCCTCGTGTT
    CAACGATCTC TTACTCACCA CTTAAAGCGT GGCCGCAGGG ACCTGGTTGA CAGTGATGGG
    AGGCTGATGC TGGATGACTC CTTGATCTAC GACAACGAGG GCGATCAGGT CAAGAACGGC
    ACCAACATGA AGTCGCTGAT CTTGGAGATG GAGGAAGCTG TGGTCGCAGC CTCCTTATCA
    CAGACGGGTG CTTCCCTCGG GAGTGCATTT GCTGCTGTCA TGCTGCTGTT GCTGGTGCTT
    CTGGGAGTCT GCCTTGTCAC CAGGAAGTGC AGGACGCTGC GGAGGAAGCG AGAGGCTGTC
    CGAGCTGCCC CAGAGGAACA CCCTCTGAAC ACTAAGGTGG AGCTGCCCAA GGGCACAGAG
    AAGGCAGTGA ACAGCCAGTA CTGCACCGTG AGGAACATCA ATATACTGAG GGAAACAGGG
    GGCATCTCCA GTGTCAAAAG CAGAAAGGTG AAGCAGGTGA ACTTAGAAGT TAAAGTCCAC
    AATAATTTGC ACGACGGAAC AGAAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_420470.4
    CDS458..12427
    Translation

    Target ORF information:

    RefSeq Version XM_420470.6
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_420470.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    ATGGGCGCCT GGCTGCCGCT GTGCCTGCTC AGCGCCGCGC TGGCCGCCGC CGCCGCCGCC 
    GCCTCAGGTG CCTGTGTCTA TCAAGGCTTG TTGTTTGAGA ATAACACAAT TTGGAAGCCA
    GATTCTTGCC AAGACTGCAG TTGCCAAAGC AATGTTGTCA CCTGTGAACC TGCTGTCTGC
    GAACATCCTC AGTGTGACTT TGAGAAGGGA GAAGTGCTCC AAATAGCCCC AAACAAATGT
    TGCCCTAAAT GTGCCTCACA AACTGAAGGA TATTGCCAGC ATGAAGGACA GATCCACAGT
    CATGGCACGC AGTGGGCCAG CTCTGGATGT GTTCTGTGCT CCTGTGCCCA TGGGAAGGTG
    AGCTGCACGC CCATAGCCTG CCCAGTCCTC ACCTGTGAAA GGGATGAGCT GCCATACACT
    GCCCAGGGAG CCTGTTGTCC CACATGCGTG GGCCTTGGTG AGCCCTGCTA CTTTGATGGC
    CGTTCGTTCC GGGATGGTGA GGACTGGCGC CTTGGTCGGT GCTCCAAATG TGTCTGCAGG
    GATGGCGTGA CTCAGTGCTT CACAGCTTCC TGTGAGGCTG TTCTGTGCAA ACAGGATGAA
    GTTTTGGCTA TGTCTCCTGG AAAATGCTGT CCAGAATGTG TTCCAAAGTC CTGCTCGGTG
    TCAGGAAAGG TGTACCAGCA CGGAGAACGG TGGAAAAAAA ATGCCTGCAG TGCGTGTGCG
    TGTCATAGAG GGGCAGTCAG ATGTCTGAGA GAAACCTGTG ACTCTGTTAT TTGTGAGAAG
    GGGGATAACA AAGTGCAGCG TTCCGGGAAG TGCTGTGAGG AGTGTGTGTC TTCCAAGGAA
    AACTGCTTGT ATGATGGCAC TATCAGGTAC CACGGTGAGA TGTGGAATAT TACTCGCTGT
    GACTTCTGCA TGTGTGATGA AGGTCAAGTG ACTTGTCACA AAGCAGAGTG CGCCAAAGTA
    GAATGTGCCA AGGGTGAAGA ATTAATTCAC TTAGATGGGA AGTGCTGTCC TGAATGTATT
    TCAAGTAATC GTCATTGTTT TTACAAAGGA CATACCAAAG CTAGCGGAGA GACGTGGAAG
    GAAGGGCTGT GCAGAGAGTG TGAATGCCGC GACTCAGAGG TGGTCTGCTT TCAGCTTTCC
    TGTCCTCCCT GCCCACCGGG CAGCATGGCT GTCAGGGAGA AGGGTGATTG CTGTCCACGC
    TGCCAGCCAG GAAAGTGCCA CCCAGACTGT TTGACCTGCT CGCAGTCCTT TGATCACTGT
    ATTGCCTGCC GTGATGTGAA GAAGCAGCTG CAGAATGGGC GCTGTGTGGA GAGCTGTGGG
    TCAGGCTTCT ATCAGGATGG GGGGACTTGC TTAGCCTGCA ATGAAACATG CTCAGCCTGT
    ACGAATGGTT TTGAGTGTTC CTCATGCCAG ACGTCCCTGC TGATGAAGAA TGGGCAGTGT
    GTGACTTCAT GTGGGAAGGG ATTCTTTCAG GATCAACTCA TATGTGCAGT TTGTCATGAG
    TCCTGTTCCT CGTGTTGGGG ACCTGCTGAC AACAGTTGTC TCTCCTGCAA AGACACGTCA
    CATGTGCTAA AAGCAGGGCT CTGCGTGGCC AGCTGTGGAA AGGGATTTTA TGAGAAGGAT
    GGAATCTGCA GTGTTTGTGA CCCGTCCTGT GAAACGTGCA ATTCAGCCCA GCCCAGTTGT
    TTAACCTGTG CTTCAGATAA GGTGTTACAC AACGGGAACT GCATTTCAGA GTGCCCAGGC
    GGATATTTCC CAGGCAGCAA TGGAAGATGC AGGGCTTGTC ATGATTCGTG CTCCACCTGT
    GAGGGACCTC TGGCCACTCA CTGTACCTCC TGTTCCCTTC CTCTGGCTTT GCACCAGGGC
    CAGTGCCTGC AGGGCTGTGG AGAGGGCTTT TATCAGGATC ACAGCATTTG CAGGGGTTGC
    CACCCATCCT GCCGTAACTG CATTGGCCCA GGGGCCTCTC ATTGCCTGCA GTGCTGGGCA
    CCAGAGGATG TGCTGCAGCC CCATCAACCT GTGGAAGGAG CTGTGCATGG CTTTTGCCTC
    TCACACTGCC AGGCCCAGTT CTACCTGGAT GTCACAGGAG TCTGCAAACA GTGCCACTCC
    TCCTGTGCAG GCTGCATAGG GAACACTTCC CAGGACTGTA CGGCCTGTCC GTCTTCGCAC
    ATGCTGCTCG AGGGCAGATG CCTCTCCGAG TGTCCAGAGG GGTTGTTCAG CCAGCAGGAT
    CACTGCTCCT CCTGCCATCC GTCATGCAAG ACATGCCATG GCCCTTCTGA TTTGGAGTGC
    TTAACCTGCC ATCCCCATGC AACTCTGGCT GCTGGAAGGT GCAGGACTAG CTGCAAGGAA
    GAGCAGTATC TCAACTTGGT TGGATATTGC GTGGACTGCC ATCCTTTGTG CCATCAGTGT
    GTGGCCAACC TGTGGGACAC TGGCAGCCTC TGTCTCAAGT GCCAGAATGC CCGTCACTTG
    CTCCTTGGGG ATCACTGCGT TCCTGACTGT CCAGCAGGAT ACTACACAGA GAGCGGCGCC
    TGCAAACGAT GTCACCCCTC ATGTAAAACC TGCAGAGGTA GGGGTCCTCT TTCCTGCTCC
    TCCTGCAACA CCAGCCTGGT TCTGTCCCAC ACCAGCATGT GCGTTCCAGT CTGTTCTCCG
    GGATACTACA AGGATCAGAG CCAGACCTGC AAACCTTGCA ACAGCCAGTG CCTGAACTGT
    GAATCAGCTA CAGGCTGTAT GTCTTGCAGA GATCCAGCTA AGGTCCTGTT ATTTGGAGAA
    TGCCAGTATG AGAGCTGTGC TCAGCAGTAT TATCTGGATT TTTCAACCAA GACTTGCAAA
    GAGTGTGACT GGAGCTGCAA TGCTTGTAAA GGCCCTCTGA GGACCGATTG CCTGCAGTGT
    ATGGAGGGTA GTGTACTCCA CAAAGGAGCT TGTGTGGAAC AGTGCCCCAC AGCTTTCTAC
    AAGGAGTCAG ACATGTGCCA GAGGTGTGAT CAACATTGCC TTCAGTGTCA CCAAGCAAAT
    GAGTGCAGCC TTTGTGAAGC CCCATTTTTC CTCTTGGACA CTCAGTGCGT TTATAAGTGT
    GGGAAGGGAT ATTATGCAGA CCACGCACAG CGAAAATGCA TAGCTTGTCC TCACGGGTGC
    TTGGAGTGTG ACAGTGACGG CCTTTGCCAC CTCTGTGATG GCAACACCTT TCTGAAAAAC
    AAGATTTGCG TTTCTGCCTG TGGACAGGGA TTCTATGGCA ATAAGTGGAC CAGAGAGTGT
    GAAGTCAGCA GGCATCCTTC AAGCTTCCGT GTAAACAGTA CCCTCACAGT AGGGATTGGT
    GGGGTAAAGC CTCTAGACCT CTCCTTACTG GATGTGCGTG ATCTGGATGG TGACACAGGA
    CAGCTCTTGT TCCATATTGA CAGCGCTCCC TCCAATGGCA GGCTAGTGAT GGTGGTGAAT
    GGGAAAGAAG CGCAGCTGAG CAAAGGTGAC CACTTCAGCT TCAAGGATGT GAAGGAGAAT
    AGAGTCCGCT TTGTGCACAG CAGAGAGAAA TCCAGGAAAG GACAGCTTTC CTTGAGAGTC
    AGCGATCAAC AGTTCTCCCA TCCTGAGGTG ATCAACATTC AAGCATTTTC AACACAGGCC
    CCCTATCTGC TGAGAAATGA GGCCCTCCTT GTCCGCAGAG GGGAAATTGG AACTATAACA
    GACCTGTTGC TTGATGTGAG AGATGGTGAC AATCCTCAGG ATGTCATGTT GATGGTTTTA
    GAGCCTCCAC GTCATGGTCA GCTGATAAAA CCTCCCAGGG ACTCAGCTTC TTCAGTCTAC
    ATGTTCCACC TCGATGACCT TGCTGGTGGA CTGCTACGTT ATGCTCACGA TGGCTCTGAC
    ACCCAGGATG ATGCAATTCT TCTGCAAGTG AACGATGGAT ACCACTTCCA GAACATCCTG
    TTCCACATTA AGATCGCTCC CAAGAGTTAC AGAAGCCCTT GGCTGGTGAC AAATTCCCTG
    GTCTGGGTGC CCCAGGGAGG CATGCTGCAG ATCTCCAAGA CAATTCTGCA CGCTGAGGTA
    CCTGGAGCCC GTGACTCTGA GATAACCTAC AACATCATCA AGGACCAGCC AAGACATGGT
    GAAGTGGTGC TTCTGGTCCC AATGCCGGCA GATGGCCCAG CAGAGTCATG GCAGCTTTTG
    CCTGATGGAA GAGCGGCCTC ACCTACAACC TCTTTTACCC AGCAGGACAT CAATAATGGC
    ATCGTGTGGT ACAGGCACTC TGGAACTCAA GTAGAGAGTG ACTCCTTCCA GTTCCAGGTG
    TCCAGCTCTG CCAGTCCACA AGCCAGCTTG GAAAGCCACG TGTTCAACGT TGCTGTGCTC
    CCACAGACAC CCAGAGCACC TCAGCTTTCC CTTGGCTCCT CTTTGCACAT GGCTGTGCTG
    GAGGACCGCG TGACAGTGAT TGAGCCCCAT CACCTCTCCT TTGTAGACCC AGAGATTCCC
    AGTGAGAAAA TCCTGTTCAA CGTGACTGTC CCTCTGCTTC CTGGCCAAGG TATTGTGGAG
    CACAGAGACA GGCCTCACTC GCCAGTCAGG TATTTCACTC AAGCAGATAT AAACCATGGC
    AAGATTGCTT ATCGTCCACC AACTGCAGCT CCACACTTGA GAGAGATCAT TGCATTCTCA
    TTTGCAGGTC TCCCAGAGTC AGTGAACTTC CGATTCACAG TGTCTGGTGG AGAACACACA
    ACCCCGGAGA TGGCGTTTGT CATTCACTTG CTCTCTGCAG AGCAGCAGCC TCCAGTCTTC
    CAGATCACAG CTCCTTTCCT GGAGGTCAGC CAGGGGGGAA AGGCCACCAT CGGTGTCCAG
    TTGGCTGTGA GTGACACAGA TACAGCTCCT GAGGGCCTCT TCTTTGAGCT GGTGAAACCT
    CCCCATCATG GCATCGTGCT CAAGCATGAT GCAGGTGTGC AGGAGCACAT GAGAGCAGGT
    GACACCTTCA CCTATGAGGA CGTCACTCGA AATGCTCTGC AGTACGTACA TGATGGCTCA
    TCGGCAGCAG AAGACAGCAT GGAAATCTCT GTCACCGATG GCGTCACCAC AGTGACCACA
    GTGCTGAGAG TGGAGGTGTC CCTACTGGAT AACAACGGCC CACAGCTGGC CCCAGGCTGC
    CTGTTGGCAA TCACTGTGGC TAGTAAAAGC TCTGCAACTC TCTCTCGACT GCACCTCGCT
    TACATTGACA ACAATTCTCC TGACTCAGAG ATAAGAATCC AGTTAATATC TCTGCCCACG
    TACGGCACCC TCTTACGGAC GTCTGGCTCT CATGCTGAAG AACTTTCTAG GGTTTATAAT
    TTCACCATGG AAGACATCAA CAACCAGAGG ATCAGTTACT CCACTGTGTT TGAGACAAGC
    AATCAGCCAG TTACGGATGC CTTTCACTTC ACTGTTTTTG ATGCTGATAA TAACCGGCTT
    GACAGACAGA TGTTTACCAT CACCATCACG TCTGCCCAGA CAGCACCATC ACTCATTGTG
    TTTGCTGACC ATATCGCTGT GGATGAGGGG GGCAGGGTCC CACTTCTGGC TCATCACCAC
    TTAACTGCTG ATTATGATAC TGCTGCCAAG GAAGACCTGA GGGTCACGGT TATCACTCTA
    CCCGTGTATG GCTACATTGA AAACACTAAG ACAGGTGAGA CTTTTGGACC ACAGAGTGCT
    GTGACCACAG ATGCATCCTT CTCCATTCAA GATGTTCTAG AGAACAACAT TTACTACTTT
    CAGAGCGTCC ATGAAAGCAT CGAGCCAACA AGTGATGTTT TCAGCTTTTA CGTGAGCGAT
    GGCTTCAGCC AGTCTGAGGT GCACAGCATC AACATCACCA TTCAGCGACG GAATGATGAG
    CCCCCAAAGA TGGTGTTGGA GCCTGTCAGA GTGCATGAGA GCTCAGTGGT TGTTACCAGT
    GCCTCACTCA ACCTGCAGGA CCTGGACACC CAGAACAGTG AGCTTGTCAT CATGGTTACC
    AAAACTCCTG AGCACGGTCA CCTTCGCAGG AGGCAGACTG CTGTGGAGCC CCCAGAGCGT
    GGACACGTGC TGTCTCTGGG CTCATCCTTC ACCTACCAGG ACATTTTGGA TGAGCTGATT
    GTTTATTCTC AAAGCGGTGC AGCAGCACAG ACGGATGAGT TTGGCTTCTC CATCACAGAT
    GGTCTCTACA TGCAGACAGG GAAGCTGGAG TTCTCCATGG AGCTTCCAAA GAAGCAGCTG
    CCCACATTGA CTGCCAGCAG GGTCCTGCAG CTCCCCACTG GCTCTGCAGC ACACATCACA
    GATCAACACT TGAAAGCAGC AGCTGTTTAT CCAGATGACA CAATGATCAG ATTTGTCATG
    AAGAGGGATC CCAGCGTGGG CCATTTGCAG TTGACTGATA TTGATAATCC AGTCGAGATC
    TCTGTCAGAG GGCCTGTCAA AAGTTTTACC CAGGCTGATA TAAATAAAGG GCAAGTGGTT
    TATAGTCACA AGAAGGGGGA TCCTGGTGGA AATTTTGCCT TCACCTTTGA TGTGATCGAT
    GCAGATGGCA GCAGACTGAT GGACCAGTTT TTTCATATCA CTGTATTAGA AGACAGGTTC
    CCCCCCTCCA TCGTCGCTAA CACAGGGCTG GTCTTAGATG AGAACTCTGC AAAGAAGATC
    ACAACACTGC AGCTCTCTGC CACTGACCGG GACAGTGAGC CGACCCAGCT CCAGTACAAG
    CTCACCAAGC AGCCACAGCT GGGGCACCTG GAGCATGTGG CCTCCCCAGG GACAAAAATC
    AGTTCTTTCA GCCAGGCAGA CTTGGCCTCT CGCAGCATCC AATACGTTCA CACTAGCGAC
    ACTGAAAAGC ATACGGATGC CTTCACCTTC TCTGTGTCTG ATGGAACAAA TGAGGTCAGC
    CAGACATTTG ACATCACCAT AAACCCTGTG GATGATTCCT TACCTGTAGT GCAAAGCTTT
    GGGATGAGAG TTCAGGAAGG TGTGAGAAAA ACCATCACAG AGTTTGAGCT TAAAGCAACA
    GATGCTGACA CAAAGGCTGA GGCAATTACA TTCACAGTCG TGCAGCCCCC TCGACATGGC
    CTGATTGAAC ACACCAGCAA TGGGCAGCAT TACCACCAAG CCACCACCTT CACCATGGAT
    GACATTTACC AGAACCGCAT CAGCTACAGT CACGATGGAA GCAACTCACT GAAGGATCGC
    TTTGCTTTCA CTGTGTCTGA TGGGACCAAT CCCTTCTTCA TTATGGAGGA TGGGGGGAAA
    AAGGTTGTGA CAGCAGCACC ACAGCGCTTC AGAATGGACA TCCTCCCTGT GGATGATGGG
    ACACCCAGGG TGGTCACCAA CCAGGGTTTG CAATGGCTGG AGTATGTGGA TGGCAAGGCA
    ACCAATCTAA TCACCAAGAA GGAATTACTG ACAACAGATC CTGACACAGA TGACAAACAG
    CTGATCTATG AGATAACAGA TGGTCCCAGG AATGGTTATG TGGAGAACAA GCTGAAACCA
    GGAACAACTG TGACCATGTT TTCTCAGGAG GATGTGAATC AGGGCCTCAT TCGGTATGTG
    ATGCATGAGG AGAAGGTTCA GGAGACTATG GACAGTTTTC AGTTCCTCGT GAAGGACAGC
    AAACCCAATG TGGTCCGTGG CAACGTCTTC CACATCCAGT GGTCTCTCCT CAGCTTTACA
    TACAGCAGCT ACAACGTCAG TGAGAGCGCT GGCTCTGTGA GCGTCACCGT GCGGCGGATT
    GGTAACCTTA ACCAGTATGC CATTGTCTTG TGCCGCACGG AGCAGGGAAC AGCCACTTCC
    AGCTCTGGTT CACAGTCTGA AGAGCAGGAC TATGTGGAGT ACGCAGGGCA GGTGCAGTTT
    GAGGAGCGGG AAGACACCAA GGCCTGCACC ATCGTCATTA ACGATGACGA CATCTTTGAG
    AGCACCGAGA GCTTCACTGT GGAGCTCAGC ATGCCAGCCT ATGCTCTGCT GGGGAATGTC
    ACCCATGCCA CAGTCACCAT CACAGACGCT GAGGATGAGC CCATGCTGCA GTTCGACAGG
    AAGACGTACC ACGTCAGTGA GAGCGCTGGG TTTCTCTCTG CGCCTGTTGA AAGGAAAGGA
    GATGCTAGCA GCACTGTGTC TGCTGTCTGC TACACTGTCC CCAAATCAGC CAAGGGAAGC
    AGCCTTTATA TGCTGGAGTC TGGCTCCGAC TTCAAGTCCC GAGGGATGAC AGATGACAGC
    CGCATAGTTT TTGGACCAGG TGTTACCATG TCGACATGTG ATGTCATGCT TATTGATGAC
    AGTGAATATG AAGAAGAAGA GGAGTTTGAG ATAGTATTGG CTGATGCTTC GGAAAATGCA
    CGCATTGGCA ACCGGGCTTC AGCCAGCGTC TTCATCGGTG GTCCCAATGA TGCCTCGACA
    GTGTTCCTGG GAAATGCCAC GTTCACCGTC AGTGAGGCTG CAGGAAACAT TGAAATTCCA
    GTCCTCCGTC ACGGACCTGA TCTTTCAACC CCCACCTCAG TGTGGTGTGC TACTCGGACA
    TCAGATCCTC CATCTGCCAG CCCAGGAGTT GATTATGTCC CCAGCTCCAG AAAAATAGAG
    TTCAGGCCAG GCAAGACAGA AGAGTTCTGC ACTCTGACAA TCCTGGATGA TGCTCAGTAC
    CCAGTGATAG AAGGGCTGGA GACATTTGTT GTTTATCTCA GCTCACCCCA TGGAGCTGAG
    CTGATAAAGC CCTTCCAAGC CATTGTGGCC ATTAATGACA TCATCCAAGA TGTACCAACC
    ATGCAGTTCA CCAAGGATGT GTTCACAGTG AAGGAAAAGG AGGGTGCACT ACACATCCCC
    ATTTTCCGCA CTGGCGACCT GAGCTATGAG TCCTCCGTGA GGTGCTACAC CCAGAGCCAG
    ACAGCAGAAG TCATGGAGGA CTTTGCAGAG AGGAGAAATG CAGACGAGTC CCGCATCACT
    TTCTTGAAAG GGGAGAAGGT GAAAAACTGC AGTGTTTACG TCAGTGATGA CTCTGCCTTT
    GAACCAGAGG AGCAGTTTCA TGTCCATCTT GGTAGTCCGC TTGGAAACCA TTGGAGTGGA
    GCTAGGATTG GGAAGATAGA CATGGCAACT GTGACCATCA CTAATGATGA AGACGCACCC
    ACCATTGAGT TTGAAGAAGC TGCGTACCAG GTACGGGAGC CACCTGGACC AGAGGGTGTC
    GCTGCTTTAA ATATCAAAGT GGTCCGGAGA GGGGATCAGA ACAGGACCTC AAAAGTGCGC
    TGTAGCACTC GTGATGGCTC AGCTCAGGCC GGAGTGGATT ACTACCCCAA GAGTCGAGTG
    CTCAAATTCA GCCCAGGTGT GGACCACATC ATGTTTAAGG TGGAGATTAT GTCCAACGAA
    GATCGGGAGT GGCACGAGTC CTTTTCTCTG GTGCTGGGTC CAGATGACCC AGTGGAAGCT
    GTTCTTGGAG ACACCAGCAC TGCAACAGTG ACTATTTTGG ATCAGGAGGC AGCAGGCAGC
    CTGATTCTGC CAGCACCTCC TGTGGTCGTC TCCTTGGCTG ATTATGACCG TGTGGAAGAA
    GCGACCAAGC ACGGTGCTAA GAAATCCCCT TCTCCAGGCT ATCCTCTCAT CTGTGTCACT
    CCCTGTGATC CTCACTTCCC CAAGTTCATG GTCATGAAGG AGCGCTGCAG CGAGGCCGGG
    ATCAACCAGT CTTCCATACA GTTCAGCTGG GAAGTAGCAA CCCCCACAGA TGGGAATGGG
    GCCAGGTCGC CTTTTGAAAC CATCACTGAC AACACACCAT TCACCAGCGT CAACCATAAG
    GTGCTGGACA GCATTTATTT CAGCCGGCGG TTCCACGTGC GCTGTGTGGC CAAGGCTGTG
    GACAAGGCTG GCCATGTGGG CACCCCCTTG AGGAGCAACG CAGTTACTAT AGGGACAGAC
    AGTGCCATTT GTCACACTCC TGTGGTCGCG GGGACAGCTC GAGGCTTCCA GGCACAGTCG
    TTTGTTGCCA CTCTGAAGTA TCTGGACGTC AAACACAAGG AGCATCCCAA CAGGATCCAC
    ATCTCAGTGC AGATCCCCCA CCAGGATGGA ATGCTGCCAC TCATCTCCAC CCTGCCCCTG
    CACAATCTGC ATTTCCTGCT CTCTGAGTCT ATCTACAGAC ACCAGCATGT CTGCTCAAAC
    CTGGTCACCA TCAGAGACCT TAAAGGCATC TCAGAGGCAG GATTCCTTGA TGAAGTGACA
    TATGATAGCA TTGTTCTCGG TCCTGGCTAT GACCGCCCTT ACCAGTTCGA CCCCTCCGTG
    AGAGAGCCAA AGACAATCCA GCTGTACAAG CACCTCAATC TGAAGAGCTG CATATGGACC
    TTTGATGCCT ATTACGACAT GACTGAATTA ATTGATGTCT GTGGAGGCTC TGTAACAGCT
    GACTTCCAGG TACGGGACTC TGCTCAGTCC TTCCTAACAG TCCACGTCCC CCTCTATGTG
    TCCTATATTT ATGTGACAGC GCCGAGAGGC TGGGCTTCTC TTGAGCATCA CACAGAGATG
    GAGTTCTCCT TTTTCTACGA CACTGTTCTC TGGAGGACAG GGATTCAGAC GGACAGTGTC
    CTCTCAGCCC GGCTGCAGAT AATCAGAATC TACATCCGTG AGGATGGCCG ACTGGTTATT
    GAGTTCAAGA CCCAGGCCAA GTTCAGAGGG CTCTTTGTAA TGGAGCACCA CAGCTTGCCA
    GATGTGAAGT CGTCTTTGAT GACTCCAGAC CATCTGGGAG GGATCGAATT TGACCTGCAA
    CTGCTGTGGA GTGCTCAGAC TTTTGACTCT CCCTACCAGC TCTGGAGGGC AACCAGCTCT
    TATAACAGGA AGGACTACTC TGGAGAGTAC ACAATCCTCT TAATTCCTTG TACTGTGCAG
    CCTACACAGC CATGGATAGA GCCTGGAGAG AAGCCCCTGC CCTGTACAGC TCATGCTCCC
    GAGCGATTTT TGATCCCAAT CGCCTTCCAG CAGACAAACC GCCCAGTTCC TGTTGTCTAC
    TCCCTAAACA CGGAGTTTCA GCTGTGTAAC AATGAGAAGG TGTTTCTGAT GGATCCCACC
    AAGTCCGAAA TGTCTCTGGC AGAAATGGAT TACAAAGGGG CTTTTTCAAA AGGGCAAATC
    CTGTATGGCC GTGTGCTCTG GAATCCTGAA CAAAACCTAA ACGCTGCTTA CAAACTGCAG
    CTGGAGAAAG TCTACCTGTG CACTGGCAAG GATGGCTACG TGCCCTTCTT TGACCCAACA
    GGAACTGTCT ATAACGAAGG GCCTCAGTAT GGCTGTATTC AACCCAACAA GCACCTGAAA
    CACAGATTTC TCCTCTTGGA CCGAAACCAA CCAGAAGTCA CAGATAAGTA TTTCCACGAT
    GTGCCTTTTG ATGCCCACTT TGCCTCTGAA CTGTCTGAGT TTCACTCAGT AAGCAGCATG
    CCTGGTGTGG ATGGATTTAC TCTCAAGGTG GATGCTCTCT ACAAGGTGGA AGCTGGACAT
    CAGTGGTACC TTCAAGTTAT CTACATAATA GGCCCAGAGA GCATTGCGGG GCCTCGTGTT
    CAACGATCTC TTACTCACCA CTTAAAGCGT GGCCGCAGGG ACCTGGTTGA CAGTGATGGG
    AGGCTGATGC TGGATGACTC CTTGATCTAC GACAACGAGG GCGATCAGGT CAAGAACGGC
    ACCAACATGA AGTCGCTGAT CTTGGAGATG GAGGAAGCTG TGGTCGCAGC CTCCTTATCA
    CAGACGGGTG CTTCCCTCGG GAGTGCATTT GCTGCTGTCA TGCTGCTGTT GCTGGTGCTT
    CTGGGAGTCT GCCTTGTCAC CAGGAAGTGC AGGACGCTGC GGAGGAAGCG AGAGGCTGTC
    CGAGCTGCCC CAGAGGAACA CCCTCTGAAC ACTAAGGTGG AGCTGCCCAA GGGCACAGAG
    AAGGCAGTGA ACAGCCAGTA CTGCACCGTG AGGAACATCA ATATACTGAG GGAAACAGGG
    GGCATCTCCA GTGTCAAAAG CAGAAAGGTG AAGCAGGTGA ACTTAGAAGT TAAAGTCCAC
    AATAATTTGC ACGACGGAAC AGAAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07890
    Clone ID Related Accession (Same CDS sequence) XM_420470.6 , XM_025150291.1 , XM_420470.5
    Accession Version XM_025150291.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11970bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product extracellular matrix protein FRAS1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    ATGGGCGCCT GGCTGCCGCT GTGCCTGCTC AGCGCCGCGC TGGCCGCCGC CGCCGCCGCC 
    GCCTCAGGTG CCTGTGTCTA TCAAGGCTTG TTGTTTGAGA ATAACACAAT TTGGAAGCCA
    GATTCTTGCC AAGACTGCAG TTGCCAAAGC AATGTTGTCA CCTGTGAACC TGCTGTCTGC
    GAACATCCTC AGTGTGACTT TGAGAAGGGA GAAGTGCTCC AAATAGCCCC AAACAAATGT
    TGCCCTAAAT GTGCCTCACA AACTGAAGGA TATTGCCAGC ATGAAGGACA GATCCACAGT
    CATGGCACGC AGTGGGCCAG CTCTGGATGT GTTCTGTGCT CCTGTGCCCA TGGGAAGGTG
    AGCTGCACGC CCATAGCCTG CCCAGTCCTC ACCTGTGAAA GGGATGAGCT GCCATACACT
    GCCCAGGGAG CCTGTTGTCC CACATGCGTG GGCCTTGGTG AGCCCTGCTA CTTTGATGGC
    CGTTCGTTCC GGGATGGTGA GGACTGGCGC CTTGGTCGGT GCTCCAAATG TGTCTGCAGG
    GATGGCGTGA CTCAGTGCTT CACAGCTTCC TGTGAGGCTG TTCTGTGCAA ACAGGATGAA
    GTTTTGGCTA TGTCTCCTGG AAAATGCTGT CCAGAATGTG TTCCAAAGTC CTGCTCGGTG
    TCAGGAAAGG TGTACCAGCA CGGAGAACGG TGGAAAAAAA ATGCCTGCAG TGCGTGTGCG
    TGTCATAGAG GGGCAGTCAG ATGTCTGAGA GAAACCTGTG ACTCTGTTAT TTGTGAGAAG
    GGGGATAACA AAGTGCAGCG TTCCGGGAAG TGCTGTGAGG AGTGTGTGTC TTCCAAGGAA
    AACTGCTTGT ATGATGGCAC TATCAGGTAC CACGGTGAGA TGTGGAATAT TACTCGCTGT
    GACTTCTGCA TGTGTGATGA AGGTCAAGTG ACTTGTCACA AAGCAGAGTG CGCCAAAGTA
    GAATGTGCCA AGGGTGAAGA ATTAATTCAC TTAGATGGGA AGTGCTGTCC TGAATGTATT
    TCAAGTAATC GTCATTGTTT TTACAAAGGA CATACCAAAG CTAGCGGAGA GACGTGGAAG
    GAAGGGCTGT GCAGAGAGTG TGAATGCCGC GACTCAGAGG TGGTCTGCTT TCAGCTTTCC
    TGTCCTCCCT GCCCACCGGG CAGCATGGCT GTCAGGGAGA AGGGTGATTG CTGTCCACGC
    TGCCAGCCAG GAAAGTGCCA CCCAGACTGT TTGACCTGCT CGCAGTCCTT TGATCACTGT
    ATTGCCTGCC GTGATGTGAA GAAGCAGCTG CAGAATGGGC GCTGTGTGGA GAGCTGTGGG
    TCAGGCTTCT ATCAGGATGG GGGGACTTGC TTAGCCTGCA ATGAAACATG CTCAGCCTGT
    ACGAATGGTT TTGAGTGTTC CTCATGCCAG ACGTCCCTGC TGATGAAGAA TGGGCAGTGT
    GTGACTTCAT GTGGGAAGGG ATTCTTTCAG GATCAACTCA TATGTGCAGT TTGTCATGAG
    TCCTGTTCCT CGTGTTGGGG ACCTGCTGAC AACAGTTGTC TCTCCTGCAA AGACACGTCA
    CATGTGCTAA AAGCAGGGCT CTGCGTGGCC AGCTGTGGAA AGGGATTTTA TGAGAAGGAT
    GGAATCTGCA GTGTTTGTGA CCCGTCCTGT GAAACGTGCA ATTCAGCCCA GCCCAGTTGT
    TTAACCTGTG CTTCAGATAA GGTGTTACAC AACGGGAACT GCATTTCAGA GTGCCCAGGC
    GGATATTTCC CAGGCAGCAA TGGAAGATGC AGGGCTTGTC ATGATTCGTG CTCCACCTGT
    GAGGGACCTC TGGCCACTCA CTGTACCTCC TGTTCCCTTC CTCTGGCTTT GCACCAGGGC
    CAGTGCCTGC AGGGCTGTGG AGAGGGCTTT TATCAGGATC ACAGCATTTG CAGGGGTTGC
    CACCCATCCT GCCGTAACTG CATTGGCCCA GGGGCCTCTC ATTGCCTGCA GTGCTGGGCA
    CCAGAGGATG TGCTGCAGCC CCATCAACCT GTGGAAGGAG CTGTGCATGG CTTTTGCCTC
    TCACACTGCC AGGCCCAGTT CTACCTGGAT GTCACAGGAG TCTGCAAACA GTGCCACTCC
    TCCTGTGCAG GCTGCATAGG GAACACTTCC CAGGACTGTA CGGCCTGTCC GTCTTCGCAC
    ATGCTGCTCG AGGGCAGATG CCTCTCCGAG TGTCCAGAGG GGTTGTTCAG CCAGCAGGAT
    CACTGCTCCT CCTGCCATCC GTCATGCAAG ACATGCCATG GCCCTTCTGA TTTGGAGTGC
    TTAACCTGCC ATCCCCATGC AACTCTGGCT GCTGGAAGGT GCAGGACTAG CTGCAAGGAA
    GAGCAGTATC TCAACTTGGT TGGATATTGC GTGGACTGCC ATCCTTTGTG CCATCAGTGT
    GTGGCCAACC TGTGGGACAC TGGCAGCCTC TGTCTCAAGT GCCAGAATGC CCGTCACTTG
    CTCCTTGGGG ATCACTGCGT TCCTGACTGT CCAGCAGGAT ACTACACAGA GAGCGGCGCC
    TGCAAACGAT GTCACCCCTC ATGTAAAACC TGCAGAGGTA GGGGTCCTCT TTCCTGCTCC
    TCCTGCAACA CCAGCCTGGT TCTGTCCCAC ACCAGCATGT GCGTTCCAGT CTGTTCTCCG
    GGATACTACA AGGATCAGAG CCAGACCTGC AAACCTTGCA ACAGCCAGTG CCTGAACTGT
    GAATCAGCTA CAGGCTGTAT GTCTTGCAGA GATCCAGCTA AGGTCCTGTT ATTTGGAGAA
    TGCCAGTATG AGAGCTGTGC TCAGCAGTAT TATCTGGATT TTTCAACCAA GACTTGCAAA
    GAGTGTGACT GGAGCTGCAA TGCTTGTAAA GGCCCTCTGA GGACCGATTG CCTGCAGTGT
    ATGGAGGGTA GTGTACTCCA CAAAGGAGCT TGTGTGGAAC AGTGCCCCAC AGCTTTCTAC
    AAGGAGTCAG ACATGTGCCA GAGGTGTGAT CAACATTGCC TTCAGTGTCA CCAAGCAAAT
    GAGTGCAGCC TTTGTGAAGC CCCATTTTTC CTCTTGGACA CTCAGTGCGT TTATAAGTGT
    GGGAAGGGAT ATTATGCAGA CCACGCACAG CGAAAATGCA TAGCTTGTCC TCACGGGTGC
    TTGGAGTGTG ACAGTGACGG CCTTTGCCAC CTCTGTGATG GCAACACCTT TCTGAAAAAC
    AAGATTTGCG TTTCTGCCTG TGGACAGGGA TTCTATGGCA ATAAGTGGAC CAGAGAGTGT
    GAAGTCAGCA GGCATCCTTC AAGCTTCCGT GTAAACAGTA CCCTCACAGT AGGGATTGGT
    GGGGTAAAGC CTCTAGACCT CTCCTTACTG GATGTGCGTG ATCTGGATGG TGACACAGGA
    CAGCTCTTGT TCCATATTGA CAGCGCTCCC TCCAATGGCA GGCTAGTGAT GGTGGTGAAT
    GGGAAAGAAG CGCAGCTGAG CAAAGGTGAC CACTTCAGCT TCAAGGATGT GAAGGAGAAT
    AGAGTCCGCT TTGTGCACAG CAGAGAGAAA TCCAGGAAAG GACAGCTTTC CTTGAGAGTC
    AGCGATCAAC AGTTCTCCCA TCCTGAGGTG ATCAACATTC AAGCATTTTC AACACAGGCC
    CCCTATCTGC TGAGAAATGA GGCCCTCCTT GTCCGCAGAG GGGAAATTGG AACTATAACA
    GACCTGTTGC TTGATGTGAG AGATGGTGAC AATCCTCAGG ATGTCATGTT GATGGTTTTA
    GAGCCTCCAC GTCATGGTCA GCTGATAAAA CCTCCCAGGG ACTCAGCTTC TTCAGTCTAC
    ATGTTCCACC TCGATGACCT TGCTGGTGGA CTGCTACGTT ATGCTCACGA TGGCTCTGAC
    ACCCAGGATG ATGCAATTCT TCTGCAAGTG AACGATGGAT ACCACTTCCA GAACATCCTG
    TTCCACATTA AGATCGCTCC CAAGAGTTAC AGAAGCCCTT GGCTGGTGAC AAATTCCCTG
    GTCTGGGTGC CCCAGGGAGG CATGCTGCAG ATCTCCAAGA CAATTCTGCA CGCTGAGGTA
    CCTGGAGCCC GTGACTCTGA GATAACCTAC AACATCATCA AGGACCAGCC AAGACATGGT
    GAAGTGGTGC TTCTGGTCCC AATGCCGGCA GATGGCCCAG CAGAGTCATG GCAGCTTTTG
    CCTGATGGAA GAGCGGCCTC ACCTACAACC TCTTTTACCC AGCAGGACAT CAATAATGGC
    ATCGTGTGGT ACAGGCACTC TGGAACTCAA GTAGAGAGTG ACTCCTTCCA GTTCCAGGTG
    TCCAGCTCTG CCAGTCCACA AGCCAGCTTG GAAAGCCACG TGTTCAACGT TGCTGTGCTC
    CCACAGACAC CCAGAGCACC TCAGCTTTCC CTTGGCTCCT CTTTGCACAT GGCTGTGCTG
    GAGGACCGCG TGACAGTGAT TGAGCCCCAT CACCTCTCCT TTGTAGACCC AGAGATTCCC
    AGTGAGAAAA TCCTGTTCAA CGTGACTGTC CCTCTGCTTC CTGGCCAAGG TATTGTGGAG
    CACAGAGACA GGCCTCACTC GCCAGTCAGG TATTTCACTC AAGCAGATAT AAACCATGGC
    AAGATTGCTT ATCGTCCACC AACTGCAGCT CCACACTTGA GAGAGATCAT TGCATTCTCA
    TTTGCAGGTC TCCCAGAGTC AGTGAACTTC CGATTCACAG TGTCTGGTGG AGAACACACA
    ACCCCGGAGA TGGCGTTTGT CATTCACTTG CTCTCTGCAG AGCAGCAGCC TCCAGTCTTC
    CAGATCACAG CTCCTTTCCT GGAGGTCAGC CAGGGGGGAA AGGCCACCAT CGGTGTCCAG
    TTGGCTGTGA GTGACACAGA TACAGCTCCT GAGGGCCTCT TCTTTGAGCT GGTGAAACCT
    CCCCATCATG GCATCGTGCT CAAGCATGAT GCAGGTGTGC AGGAGCACAT GAGAGCAGGT
    GACACCTTCA CCTATGAGGA CGTCACTCGA AATGCTCTGC AGTACGTACA TGATGGCTCA
    TCGGCAGCAG AAGACAGCAT GGAAATCTCT GTCACCGATG GCGTCACCAC AGTGACCACA
    GTGCTGAGAG TGGAGGTGTC CCTACTGGAT AACAACGGCC CACAGCTGGC CCCAGGCTGC
    CTGTTGGCAA TCACTGTGGC TAGTAAAAGC TCTGCAACTC TCTCTCGACT GCACCTCGCT
    TACATTGACA ACAATTCTCC TGACTCAGAG ATAAGAATCC AGTTAATATC TCTGCCCACG
    TACGGCACCC TCTTACGGAC GTCTGGCTCT CATGCTGAAG AACTTTCTAG GGTTTATAAT
    TTCACCATGG AAGACATCAA CAACCAGAGG ATCAGTTACT CCACTGTGTT TGAGACAAGC
    AATCAGCCAG TTACGGATGC CTTTCACTTC ACTGTTTTTG ATGCTGATAA TAACCGGCTT
    GACAGACAGA TGTTTACCAT CACCATCACG TCTGCCCAGA CAGCACCATC ACTCATTGTG
    TTTGCTGACC ATATCGCTGT GGATGAGGGG GGCAGGGTCC CACTTCTGGC TCATCACCAC
    TTAACTGCTG ATTATGATAC TGCTGCCAAG GAAGACCTGA GGGTCACGGT TATCACTCTA
    CCCGTGTATG GCTACATTGA AAACACTAAG ACAGGTGAGA CTTTTGGACC ACAGAGTGCT
    GTGACCACAG ATGCATCCTT CTCCATTCAA GATGTTCTAG AGAACAACAT TTACTACTTT
    CAGAGCGTCC ATGAAAGCAT CGAGCCAACA AGTGATGTTT TCAGCTTTTA CGTGAGCGAT
    GGCTTCAGCC AGTCTGAGGT GCACAGCATC AACATCACCA TTCAGCGACG GAATGATGAG
    CCCCCAAAGA TGGTGTTGGA GCCTGTCAGA GTGCATGAGA GCTCAGTGGT TGTTACCAGT
    GCCTCACTCA ACCTGCAGGA CCTGGACACC CAGAACAGTG AGCTTGTCAT CATGGTTACC
    AAAACTCCTG AGCACGGTCA CCTTCGCAGG AGGCAGACTG CTGTGGAGCC CCCAGAGCGT
    GGACACGTGC TGTCTCTGGG CTCATCCTTC ACCTACCAGG ACATTTTGGA TGAGCTGATT
    GTTTATTCTC AAAGCGGTGC AGCAGCACAG ACGGATGAGT TTGGCTTCTC CATCACAGAT
    GGTCTCTACA TGCAGACAGG GAAGCTGGAG TTCTCCATGG AGCTTCCAAA GAAGCAGCTG
    CCCACATTGA CTGCCAGCAG GGTCCTGCAG CTCCCCACTG GCTCTGCAGC ACACATCACA
    GATCAACACT TGAAAGCAGC AGCTGTTTAT CCAGATGACA CAATGATCAG ATTTGTCATG
    AAGAGGGATC CCAGCGTGGG CCATTTGCAG TTGACTGATA TTGATAATCC AGTCGAGATC
    TCTGTCAGAG GGCCTGTCAA AAGTTTTACC CAGGCTGATA TAAATAAAGG GCAAGTGGTT
    TATAGTCACA AGAAGGGGGA TCCTGGTGGA AATTTTGCCT TCACCTTTGA TGTGATCGAT
    GCAGATGGCA GCAGACTGAT GGACCAGTTT TTTCATATCA CTGTATTAGA AGACAGGTTC
    CCCCCCTCCA TCGTCGCTAA CACAGGGCTG GTCTTAGATG AGAACTCTGC AAAGAAGATC
    ACAACACTGC AGCTCTCTGC CACTGACCGG GACAGTGAGC CGACCCAGCT CCAGTACAAG
    CTCACCAAGC AGCCACAGCT GGGGCACCTG GAGCATGTGG CCTCCCCAGG GACAAAAATC
    AGTTCTTTCA GCCAGGCAGA CTTGGCCTCT CGCAGCATCC AATACGTTCA CACTAGCGAC
    ACTGAAAAGC ATACGGATGC CTTCACCTTC TCTGTGTCTG ATGGAACAAA TGAGGTCAGC
    CAGACATTTG ACATCACCAT AAACCCTGTG GATGATTCCT TACCTGTAGT GCAAAGCTTT
    GGGATGAGAG TTCAGGAAGG TGTGAGAAAA ACCATCACAG AGTTTGAGCT TAAAGCAACA
    GATGCTGACA CAAAGGCTGA GGCAATTACA TTCACAGTCG TGCAGCCCCC TCGACATGGC
    CTGATTGAAC ACACCAGCAA TGGGCAGCAT TACCACCAAG CCACCACCTT CACCATGGAT
    GACATTTACC AGAACCGCAT CAGCTACAGT CACGATGGAA GCAACTCACT GAAGGATCGC
    TTTGCTTTCA CTGTGTCTGA TGGGACCAAT CCCTTCTTCA TTATGGAGGA TGGGGGGAAA
    AAGGTTGTGA CAGCAGCACC ACAGCGCTTC AGAATGGACA TCCTCCCTGT GGATGATGGG
    ACACCCAGGG TGGTCACCAA CCAGGGTTTG CAATGGCTGG AGTATGTGGA TGGCAAGGCA
    ACCAATCTAA TCACCAAGAA GGAATTACTG ACAACAGATC CTGACACAGA TGACAAACAG
    CTGATCTATG AGATAACAGA TGGTCCCAGG AATGGTTATG TGGAGAACAA GCTGAAACCA
    GGAACAACTG TGACCATGTT TTCTCAGGAG GATGTGAATC AGGGCCTCAT TCGGTATGTG
    ATGCATGAGG AGAAGGTTCA GGAGACTATG GACAGTTTTC AGTTCCTCGT GAAGGACAGC
    AAACCCAATG TGGTCCGTGG CAACGTCTTC CACATCCAGT GGTCTCTCCT CAGCTTTACA
    TACAGCAGCT ACAACGTCAG TGAGAGCGCT GGCTCTGTGA GCGTCACCGT GCGGCGGATT
    GGTAACCTTA ACCAGTATGC CATTGTCTTG TGCCGCACGG AGCAGGGAAC AGCCACTTCC
    AGCTCTGGTT CACAGTCTGA AGAGCAGGAC TATGTGGAGT ACGCAGGGCA GGTGCAGTTT
    GAGGAGCGGG AAGACACCAA GGCCTGCACC ATCGTCATTA ACGATGACGA CATCTTTGAG
    AGCACCGAGA GCTTCACTGT GGAGCTCAGC ATGCCAGCCT ATGCTCTGCT GGGGAATGTC
    ACCCATGCCA CAGTCACCAT CACAGACGCT GAGGATGAGC CCATGCTGCA GTTCGACAGG
    AAGACGTACC ACGTCAGTGA GAGCGCTGGG TTTCTCTCTG CGCCTGTTGA AAGGAAAGGA
    GATGCTAGCA GCACTGTGTC TGCTGTCTGC TACACTGTCC CCAAATCAGC CAAGGGAAGC
    AGCCTTTATA TGCTGGAGTC TGGCTCCGAC TTCAAGTCCC GAGGGATGAC AGATGACAGC
    CGCATAGTTT TTGGACCAGG TGTTACCATG TCGACATGTG ATGTCATGCT TATTGATGAC
    AGTGAATATG AAGAAGAAGA GGAGTTTGAG ATAGTATTGG CTGATGCTTC GGAAAATGCA
    CGCATTGGCA ACCGGGCTTC AGCCAGCGTC TTCATCGGTG GTCCCAATGA TGCCTCGACA
    GTGTTCCTGG GAAATGCCAC GTTCACCGTC AGTGAGGCTG CAGGAAACAT TGAAATTCCA
    GTCCTCCGTC ACGGACCTGA TCTTTCAACC CCCACCTCAG TGTGGTGTGC TACTCGGACA
    TCAGATCCTC CATCTGCCAG CCCAGGAGTT GATTATGTCC CCAGCTCCAG AAAAATAGAG
    TTCAGGCCAG GCAAGACAGA AGAGTTCTGC ACTCTGACAA TCCTGGATGA TGCTCAGTAC
    CCAGTGATAG AAGGGCTGGA GACATTTGTT GTTTATCTCA GCTCACCCCA TGGAGCTGAG
    CTGATAAAGC CCTTCCAAGC CATTGTGGCC ATTAATGACA TCATCCAAGA TGTACCAACC
    ATGCAGTTCA CCAAGGATGT GTTCACAGTG AAGGAAAAGG AGGGTGCACT ACACATCCCC
    ATTTTCCGCA CTGGCGACCT GAGCTATGAG TCCTCCGTGA GGTGCTACAC CCAGAGCCAG
    ACAGCAGAAG TCATGGAGGA CTTTGCAGAG AGGAGAAATG CAGACGAGTC CCGCATCACT
    TTCTTGAAAG GGGAGAAGGT GAAAAACTGC AGTGTTTACG TCAGTGATGA CTCTGCCTTT
    GAACCAGAGG AGCAGTTTCA TGTCCATCTT GGTAGTCCGC TTGGAAACCA TTGGAGTGGA
    GCTAGGATTG GGAAGATAGA CATGGCAACT GTGACCATCA CTAATGATGA AGACGCACCC
    ACCATTGAGT TTGAAGAAGC TGCGTACCAG GTACGGGAGC CACCTGGACC AGAGGGTGTC
    GCTGCTTTAA ATATCAAAGT GGTCCGGAGA GGGGATCAGA ACAGGACCTC AAAAGTGCGC
    TGTAGCACTC GTGATGGCTC AGCTCAGGCC GGAGTGGATT ACTACCCCAA GAGTCGAGTG
    CTCAAATTCA GCCCAGGTGT GGACCACATC ATGTTTAAGG TGGAGATTAT GTCCAACGAA
    GATCGGGAGT GGCACGAGTC CTTTTCTCTG GTGCTGGGTC CAGATGACCC AGTGGAAGCT
    GTTCTTGGAG ACACCAGCAC TGCAACAGTG ACTATTTTGG ATCAGGAGGC AGCAGGCAGC
    CTGATTCTGC CAGCACCTCC TGTGGTCGTC TCCTTGGCTG ATTATGACCG TGTGGAAGAA
    GCGACCAAGC ACGGTGCTAA GAAATCCCCT TCTCCAGGCT ATCCTCTCAT CTGTGTCACT
    CCCTGTGATC CTCACTTCCC CAAGTTCATG GTCATGAAGG AGCGCTGCAG CGAGGCCGGG
    ATCAACCAGT CTTCCATACA GTTCAGCTGG GAAGTAGCAA CCCCCACAGA TGGGAATGGG
    GCCAGGTCGC CTTTTGAAAC CATCACTGAC AACACACCAT TCACCAGCGT CAACCATAAG
    GTGCTGGACA GCATTTATTT CAGCCGGCGG TTCCACGTGC GCTGTGTGGC CAAGGCTGTG
    GACAAGGCTG GCCATGTGGG CACCCCCTTG AGGAGCAACG CAGTTACTAT AGGGACAGAC
    AGTGCCATTT GTCACACTCC TGTGGTCGCG GGGACAGCTC GAGGCTTCCA GGCACAGTCG
    TTTGTTGCCA CTCTGAAGTA TCTGGACGTC AAACACAAGG AGCATCCCAA CAGGATCCAC
    ATCTCAGTGC AGATCCCCCA CCAGGATGGA ATGCTGCCAC TCATCTCCAC CCTGCCCCTG
    CACAATCTGC ATTTCCTGCT CTCTGAGTCT ATCTACAGAC ACCAGCATGT CTGCTCAAAC
    CTGGTCACCA TCAGAGACCT TAAAGGCATC TCAGAGGCAG GATTCCTTGA TGAAGTGACA
    TATGATAGCA TTGTTCTCGG TCCTGGCTAT GACCGCCCTT ACCAGTTCGA CCCCTCCGTG
    AGAGAGCCAA AGACAATCCA GCTGTACAAG CACCTCAATC TGAAGAGCTG CATATGGACC
    TTTGATGCCT ATTACGACAT GACTGAATTA ATTGATGTCT GTGGAGGCTC TGTAACAGCT
    GACTTCCAGG TACGGGACTC TGCTCAGTCC TTCCTAACAG TCCACGTCCC CCTCTATGTG
    TCCTATATTT ATGTGACAGC GCCGAGAGGC TGGGCTTCTC TTGAGCATCA CACAGAGATG
    GAGTTCTCCT TTTTCTACGA CACTGTTCTC TGGAGGACAG GGATTCAGAC GGACAGTGTC
    CTCTCAGCCC GGCTGCAGAT AATCAGAATC TACATCCGTG AGGATGGCCG ACTGGTTATT
    GAGTTCAAGA CCCAGGCCAA GTTCAGAGGG CTCTTTGTAA TGGAGCACCA CAGCTTGCCA
    GATGTGAAGT CGTCTTTGAT GACTCCAGAC CATCTGGGAG GGATCGAATT TGACCTGCAA
    CTGCTGTGGA GTGCTCAGAC TTTTGACTCT CCCTACCAGC TCTGGAGGGC AACCAGCTCT
    TATAACAGGA AGGACTACTC TGGAGAGTAC ACAATCCTCT TAATTCCTTG TACTGTGCAG
    CCTACACAGC CATGGATAGA GCCTGGAGAG AAGCCCCTGC CCTGTACAGC TCATGCTCCC
    GAGCGATTTT TGATCCCAAT CGCCTTCCAG CAGACAAACC GCCCAGTTCC TGTTGTCTAC
    TCCCTAAACA CGGAGTTTCA GCTGTGTAAC AATGAGAAGG TGTTTCTGAT GGATCCCACC
    AAGTCCGAAA TGTCTCTGGC AGAAATGGAT TACAAAGGGG CTTTTTCAAA AGGGCAAATC
    CTGTATGGCC GTGTGCTCTG GAATCCTGAA CAAAACCTAA ACGCTGCTTA CAAACTGCAG
    CTGGAGAAAG TCTACCTGTG CACTGGCAAG GATGGCTACG TGCCCTTCTT TGACCCAACA
    GGAACTGTCT ATAACGAAGG GCCTCAGTAT GGCTGTATTC AACCCAACAA GCACCTGAAA
    CACAGATTTC TCCTCTTGGA CCGAAACCAA CCAGAAGTCA CAGATAAGTA TTTCCACGAT
    GTGCCTTTTG ATGCCCACTT TGCCTCTGAA CTGTCTGAGT TTCACTCAGT AAGCAGCATG
    CCTGGTGTGG ATGGATTTAC TCTCAAGGTG GATGCTCTCT ACAAGGTGGA AGCTGGACAT
    CAGTGGTACC TTCAAGTTAT CTACATAATA GGCCCAGAGA GCATTGCGGG GCCTCGTGTT
    CAACGATCTC TTACTCACCA CTTAAAGCGT GGCCGCAGGG ACCTGGTTGA CAGTGATGGG
    AGGCTGATGC TGGATGACTC CTTGATCTAC GACAACGAGG GCGATCAGGT CAAGAACGGC
    ACCAACATGA AGTCGCTGAT CTTGGAGATG GAGGAAGCTG TGGTCGCAGC CTCCTTATCA
    CAGACGGGTG CTTCCCTCGG GAGTGCATTT GCTGCTGTCA TGCTGCTGTT GCTGGTGCTT
    CTGGGAGTCT GCCTTGTCAC CAGGAAGTGC AGGACGCTGC GGAGGAAGCG AGAGGCTGTC
    CGAGCTGCCC CAGAGGAACA CCCTCTGAAC ACTAAGGTGG AGCTGCCCAA GGGCACAGAG
    AAGGCAGTGA ACAGCCAGTA CTGCACCGTG AGGAACATCA ATATACTGAG GGAAACAGGG
    GGCATCTCCA GTGTCAAAAG CAGAAAGGTG AAGCAGGTGA ACTTAGAAGT TAAAGTCCAC
    AATAATTTGC ACGACGGAAC AGAAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025006059.1
    CDS3955..15924
    Translation

    Target ORF information:

    RefSeq Version XM_025150291.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025150291.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    ATGGGCGCCT GGCTGCCGCT GTGCCTGCTC AGCGCCGCGC TGGCCGCCGC CGCCGCCGCC 
    GCCTCAGGTG CCTGTGTCTA TCAAGGCTTG TTGTTTGAGA ATAACACAAT TTGGAAGCCA
    GATTCTTGCC AAGACTGCAG TTGCCAAAGC AATGTTGTCA CCTGTGAACC TGCTGTCTGC
    GAACATCCTC AGTGTGACTT TGAGAAGGGA GAAGTGCTCC AAATAGCCCC AAACAAATGT
    TGCCCTAAAT GTGCCTCACA AACTGAAGGA TATTGCCAGC ATGAAGGACA GATCCACAGT
    CATGGCACGC AGTGGGCCAG CTCTGGATGT GTTCTGTGCT CCTGTGCCCA TGGGAAGGTG
    AGCTGCACGC CCATAGCCTG CCCAGTCCTC ACCTGTGAAA GGGATGAGCT GCCATACACT
    GCCCAGGGAG CCTGTTGTCC CACATGCGTG GGCCTTGGTG AGCCCTGCTA CTTTGATGGC
    CGTTCGTTCC GGGATGGTGA GGACTGGCGC CTTGGTCGGT GCTCCAAATG TGTCTGCAGG
    GATGGCGTGA CTCAGTGCTT CACAGCTTCC TGTGAGGCTG TTCTGTGCAA ACAGGATGAA
    GTTTTGGCTA TGTCTCCTGG AAAATGCTGT CCAGAATGTG TTCCAAAGTC CTGCTCGGTG
    TCAGGAAAGG TGTACCAGCA CGGAGAACGG TGGAAAAAAA ATGCCTGCAG TGCGTGTGCG
    TGTCATAGAG GGGCAGTCAG ATGTCTGAGA GAAACCTGTG ACTCTGTTAT TTGTGAGAAG
    GGGGATAACA AAGTGCAGCG TTCCGGGAAG TGCTGTGAGG AGTGTGTGTC TTCCAAGGAA
    AACTGCTTGT ATGATGGCAC TATCAGGTAC CACGGTGAGA TGTGGAATAT TACTCGCTGT
    GACTTCTGCA TGTGTGATGA AGGTCAAGTG ACTTGTCACA AAGCAGAGTG CGCCAAAGTA
    GAATGTGCCA AGGGTGAAGA ATTAATTCAC TTAGATGGGA AGTGCTGTCC TGAATGTATT
    TCAAGTAATC GTCATTGTTT TTACAAAGGA CATACCAAAG CTAGCGGAGA GACGTGGAAG
    GAAGGGCTGT GCAGAGAGTG TGAATGCCGC GACTCAGAGG TGGTCTGCTT TCAGCTTTCC
    TGTCCTCCCT GCCCACCGGG CAGCATGGCT GTCAGGGAGA AGGGTGATTG CTGTCCACGC
    TGCCAGCCAG GAAAGTGCCA CCCAGACTGT TTGACCTGCT CGCAGTCCTT TGATCACTGT
    ATTGCCTGCC GTGATGTGAA GAAGCAGCTG CAGAATGGGC GCTGTGTGGA GAGCTGTGGG
    TCAGGCTTCT ATCAGGATGG GGGGACTTGC TTAGCCTGCA ATGAAACATG CTCAGCCTGT
    ACGAATGGTT TTGAGTGTTC CTCATGCCAG ACGTCCCTGC TGATGAAGAA TGGGCAGTGT
    GTGACTTCAT GTGGGAAGGG ATTCTTTCAG GATCAACTCA TATGTGCAGT TTGTCATGAG
    TCCTGTTCCT CGTGTTGGGG ACCTGCTGAC AACAGTTGTC TCTCCTGCAA AGACACGTCA
    CATGTGCTAA AAGCAGGGCT CTGCGTGGCC AGCTGTGGAA AGGGATTTTA TGAGAAGGAT
    GGAATCTGCA GTGTTTGTGA CCCGTCCTGT GAAACGTGCA ATTCAGCCCA GCCCAGTTGT
    TTAACCTGTG CTTCAGATAA GGTGTTACAC AACGGGAACT GCATTTCAGA GTGCCCAGGC
    GGATATTTCC CAGGCAGCAA TGGAAGATGC AGGGCTTGTC ATGATTCGTG CTCCACCTGT
    GAGGGACCTC TGGCCACTCA CTGTACCTCC TGTTCCCTTC CTCTGGCTTT GCACCAGGGC
    CAGTGCCTGC AGGGCTGTGG AGAGGGCTTT TATCAGGATC ACAGCATTTG CAGGGGTTGC
    CACCCATCCT GCCGTAACTG CATTGGCCCA GGGGCCTCTC ATTGCCTGCA GTGCTGGGCA
    CCAGAGGATG TGCTGCAGCC CCATCAACCT GTGGAAGGAG CTGTGCATGG CTTTTGCCTC
    TCACACTGCC AGGCCCAGTT CTACCTGGAT GTCACAGGAG TCTGCAAACA GTGCCACTCC
    TCCTGTGCAG GCTGCATAGG GAACACTTCC CAGGACTGTA CGGCCTGTCC GTCTTCGCAC
    ATGCTGCTCG AGGGCAGATG CCTCTCCGAG TGTCCAGAGG GGTTGTTCAG CCAGCAGGAT
    CACTGCTCCT CCTGCCATCC GTCATGCAAG ACATGCCATG GCCCTTCTGA TTTGGAGTGC
    TTAACCTGCC ATCCCCATGC AACTCTGGCT GCTGGAAGGT GCAGGACTAG CTGCAAGGAA
    GAGCAGTATC TCAACTTGGT TGGATATTGC GTGGACTGCC ATCCTTTGTG CCATCAGTGT
    GTGGCCAACC TGTGGGACAC TGGCAGCCTC TGTCTCAAGT GCCAGAATGC CCGTCACTTG
    CTCCTTGGGG ATCACTGCGT TCCTGACTGT CCAGCAGGAT ACTACACAGA GAGCGGCGCC
    TGCAAACGAT GTCACCCCTC ATGTAAAACC TGCAGAGGTA GGGGTCCTCT TTCCTGCTCC
    TCCTGCAACA CCAGCCTGGT TCTGTCCCAC ACCAGCATGT GCGTTCCAGT CTGTTCTCCG
    GGATACTACA AGGATCAGAG CCAGACCTGC AAACCTTGCA ACAGCCAGTG CCTGAACTGT
    GAATCAGCTA CAGGCTGTAT GTCTTGCAGA GATCCAGCTA AGGTCCTGTT ATTTGGAGAA
    TGCCAGTATG AGAGCTGTGC TCAGCAGTAT TATCTGGATT TTTCAACCAA GACTTGCAAA
    GAGTGTGACT GGAGCTGCAA TGCTTGTAAA GGCCCTCTGA GGACCGATTG CCTGCAGTGT
    ATGGAGGGTA GTGTACTCCA CAAAGGAGCT TGTGTGGAAC AGTGCCCCAC AGCTTTCTAC
    AAGGAGTCAG ACATGTGCCA GAGGTGTGAT CAACATTGCC TTCAGTGTCA CCAAGCAAAT
    GAGTGCAGCC TTTGTGAAGC CCCATTTTTC CTCTTGGACA CTCAGTGCGT TTATAAGTGT
    GGGAAGGGAT ATTATGCAGA CCACGCACAG CGAAAATGCA TAGCTTGTCC TCACGGGTGC
    TTGGAGTGTG ACAGTGACGG CCTTTGCCAC CTCTGTGATG GCAACACCTT TCTGAAAAAC
    AAGATTTGCG TTTCTGCCTG TGGACAGGGA TTCTATGGCA ATAAGTGGAC CAGAGAGTGT
    GAAGTCAGCA GGCATCCTTC AAGCTTCCGT GTAAACAGTA CCCTCACAGT AGGGATTGGT
    GGGGTAAAGC CTCTAGACCT CTCCTTACTG GATGTGCGTG ATCTGGATGG TGACACAGGA
    CAGCTCTTGT TCCATATTGA CAGCGCTCCC TCCAATGGCA GGCTAGTGAT GGTGGTGAAT
    GGGAAAGAAG CGCAGCTGAG CAAAGGTGAC CACTTCAGCT TCAAGGATGT GAAGGAGAAT
    AGAGTCCGCT TTGTGCACAG CAGAGAGAAA TCCAGGAAAG GACAGCTTTC CTTGAGAGTC
    AGCGATCAAC AGTTCTCCCA TCCTGAGGTG ATCAACATTC AAGCATTTTC AACACAGGCC
    CCCTATCTGC TGAGAAATGA GGCCCTCCTT GTCCGCAGAG GGGAAATTGG AACTATAACA
    GACCTGTTGC TTGATGTGAG AGATGGTGAC AATCCTCAGG ATGTCATGTT GATGGTTTTA
    GAGCCTCCAC GTCATGGTCA GCTGATAAAA CCTCCCAGGG ACTCAGCTTC TTCAGTCTAC
    ATGTTCCACC TCGATGACCT TGCTGGTGGA CTGCTACGTT ATGCTCACGA TGGCTCTGAC
    ACCCAGGATG ATGCAATTCT TCTGCAAGTG AACGATGGAT ACCACTTCCA GAACATCCTG
    TTCCACATTA AGATCGCTCC CAAGAGTTAC AGAAGCCCTT GGCTGGTGAC AAATTCCCTG
    GTCTGGGTGC CCCAGGGAGG CATGCTGCAG ATCTCCAAGA CAATTCTGCA CGCTGAGGTA
    CCTGGAGCCC GTGACTCTGA GATAACCTAC AACATCATCA AGGACCAGCC AAGACATGGT
    GAAGTGGTGC TTCTGGTCCC AATGCCGGCA GATGGCCCAG CAGAGTCATG GCAGCTTTTG
    CCTGATGGAA GAGCGGCCTC ACCTACAACC TCTTTTACCC AGCAGGACAT CAATAATGGC
    ATCGTGTGGT ACAGGCACTC TGGAACTCAA GTAGAGAGTG ACTCCTTCCA GTTCCAGGTG
    TCCAGCTCTG CCAGTCCACA AGCCAGCTTG GAAAGCCACG TGTTCAACGT TGCTGTGCTC
    CCACAGACAC CCAGAGCACC TCAGCTTTCC CTTGGCTCCT CTTTGCACAT GGCTGTGCTG
    GAGGACCGCG TGACAGTGAT TGAGCCCCAT CACCTCTCCT TTGTAGACCC AGAGATTCCC
    AGTGAGAAAA TCCTGTTCAA CGTGACTGTC CCTCTGCTTC CTGGCCAAGG TATTGTGGAG
    CACAGAGACA GGCCTCACTC GCCAGTCAGG TATTTCACTC AAGCAGATAT AAACCATGGC
    AAGATTGCTT ATCGTCCACC AACTGCAGCT CCACACTTGA GAGAGATCAT TGCATTCTCA
    TTTGCAGGTC TCCCAGAGTC AGTGAACTTC CGATTCACAG TGTCTGGTGG AGAACACACA
    ACCCCGGAGA TGGCGTTTGT CATTCACTTG CTCTCTGCAG AGCAGCAGCC TCCAGTCTTC
    CAGATCACAG CTCCTTTCCT GGAGGTCAGC CAGGGGGGAA AGGCCACCAT CGGTGTCCAG
    TTGGCTGTGA GTGACACAGA TACAGCTCCT GAGGGCCTCT TCTTTGAGCT GGTGAAACCT
    CCCCATCATG GCATCGTGCT CAAGCATGAT GCAGGTGTGC AGGAGCACAT GAGAGCAGGT
    GACACCTTCA CCTATGAGGA CGTCACTCGA AATGCTCTGC AGTACGTACA TGATGGCTCA
    TCGGCAGCAG AAGACAGCAT GGAAATCTCT GTCACCGATG GCGTCACCAC AGTGACCACA
    GTGCTGAGAG TGGAGGTGTC CCTACTGGAT AACAACGGCC CACAGCTGGC CCCAGGCTGC
    CTGTTGGCAA TCACTGTGGC TAGTAAAAGC TCTGCAACTC TCTCTCGACT GCACCTCGCT
    TACATTGACA ACAATTCTCC TGACTCAGAG ATAAGAATCC AGTTAATATC TCTGCCCACG
    TACGGCACCC TCTTACGGAC GTCTGGCTCT CATGCTGAAG AACTTTCTAG GGTTTATAAT
    TTCACCATGG AAGACATCAA CAACCAGAGG ATCAGTTACT CCACTGTGTT TGAGACAAGC
    AATCAGCCAG TTACGGATGC CTTTCACTTC ACTGTTTTTG ATGCTGATAA TAACCGGCTT
    GACAGACAGA TGTTTACCAT CACCATCACG TCTGCCCAGA CAGCACCATC ACTCATTGTG
    TTTGCTGACC ATATCGCTGT GGATGAGGGG GGCAGGGTCC CACTTCTGGC TCATCACCAC
    TTAACTGCTG ATTATGATAC TGCTGCCAAG GAAGACCTGA GGGTCACGGT TATCACTCTA
    CCCGTGTATG GCTACATTGA AAACACTAAG ACAGGTGAGA CTTTTGGACC ACAGAGTGCT
    GTGACCACAG ATGCATCCTT CTCCATTCAA GATGTTCTAG AGAACAACAT TTACTACTTT
    CAGAGCGTCC ATGAAAGCAT CGAGCCAACA AGTGATGTTT TCAGCTTTTA CGTGAGCGAT
    GGCTTCAGCC AGTCTGAGGT GCACAGCATC AACATCACCA TTCAGCGACG GAATGATGAG
    CCCCCAAAGA TGGTGTTGGA GCCTGTCAGA GTGCATGAGA GCTCAGTGGT TGTTACCAGT
    GCCTCACTCA ACCTGCAGGA CCTGGACACC CAGAACAGTG AGCTTGTCAT CATGGTTACC
    AAAACTCCTG AGCACGGTCA CCTTCGCAGG AGGCAGACTG CTGTGGAGCC CCCAGAGCGT
    GGACACGTGC TGTCTCTGGG CTCATCCTTC ACCTACCAGG ACATTTTGGA TGAGCTGATT
    GTTTATTCTC AAAGCGGTGC AGCAGCACAG ACGGATGAGT TTGGCTTCTC CATCACAGAT
    GGTCTCTACA TGCAGACAGG GAAGCTGGAG TTCTCCATGG AGCTTCCAAA GAAGCAGCTG
    CCCACATTGA CTGCCAGCAG GGTCCTGCAG CTCCCCACTG GCTCTGCAGC ACACATCACA
    GATCAACACT TGAAAGCAGC AGCTGTTTAT CCAGATGACA CAATGATCAG ATTTGTCATG
    AAGAGGGATC CCAGCGTGGG CCATTTGCAG TTGACTGATA TTGATAATCC AGTCGAGATC
    TCTGTCAGAG GGCCTGTCAA AAGTTTTACC CAGGCTGATA TAAATAAAGG GCAAGTGGTT
    TATAGTCACA AGAAGGGGGA TCCTGGTGGA AATTTTGCCT TCACCTTTGA TGTGATCGAT
    GCAGATGGCA GCAGACTGAT GGACCAGTTT TTTCATATCA CTGTATTAGA AGACAGGTTC
    CCCCCCTCCA TCGTCGCTAA CACAGGGCTG GTCTTAGATG AGAACTCTGC AAAGAAGATC
    ACAACACTGC AGCTCTCTGC CACTGACCGG GACAGTGAGC CGACCCAGCT CCAGTACAAG
    CTCACCAAGC AGCCACAGCT GGGGCACCTG GAGCATGTGG CCTCCCCAGG GACAAAAATC
    AGTTCTTTCA GCCAGGCAGA CTTGGCCTCT CGCAGCATCC AATACGTTCA CACTAGCGAC
    ACTGAAAAGC ATACGGATGC CTTCACCTTC TCTGTGTCTG ATGGAACAAA TGAGGTCAGC
    CAGACATTTG ACATCACCAT AAACCCTGTG GATGATTCCT TACCTGTAGT GCAAAGCTTT
    GGGATGAGAG TTCAGGAAGG TGTGAGAAAA ACCATCACAG AGTTTGAGCT TAAAGCAACA
    GATGCTGACA CAAAGGCTGA GGCAATTACA TTCACAGTCG TGCAGCCCCC TCGACATGGC
    CTGATTGAAC ACACCAGCAA TGGGCAGCAT TACCACCAAG CCACCACCTT CACCATGGAT
    GACATTTACC AGAACCGCAT CAGCTACAGT CACGATGGAA GCAACTCACT GAAGGATCGC
    TTTGCTTTCA CTGTGTCTGA TGGGACCAAT CCCTTCTTCA TTATGGAGGA TGGGGGGAAA
    AAGGTTGTGA CAGCAGCACC ACAGCGCTTC AGAATGGACA TCCTCCCTGT GGATGATGGG
    ACACCCAGGG TGGTCACCAA CCAGGGTTTG CAATGGCTGG AGTATGTGGA TGGCAAGGCA
    ACCAATCTAA TCACCAAGAA GGAATTACTG ACAACAGATC CTGACACAGA TGACAAACAG
    CTGATCTATG AGATAACAGA TGGTCCCAGG AATGGTTATG TGGAGAACAA GCTGAAACCA
    GGAACAACTG TGACCATGTT TTCTCAGGAG GATGTGAATC AGGGCCTCAT TCGGTATGTG
    ATGCATGAGG AGAAGGTTCA GGAGACTATG GACAGTTTTC AGTTCCTCGT GAAGGACAGC
    AAACCCAATG TGGTCCGTGG CAACGTCTTC CACATCCAGT GGTCTCTCCT CAGCTTTACA
    TACAGCAGCT ACAACGTCAG TGAGAGCGCT GGCTCTGTGA GCGTCACCGT GCGGCGGATT
    GGTAACCTTA ACCAGTATGC CATTGTCTTG TGCCGCACGG AGCAGGGAAC AGCCACTTCC
    AGCTCTGGTT CACAGTCTGA AGAGCAGGAC TATGTGGAGT ACGCAGGGCA GGTGCAGTTT
    GAGGAGCGGG AAGACACCAA GGCCTGCACC ATCGTCATTA ACGATGACGA CATCTTTGAG
    AGCACCGAGA GCTTCACTGT GGAGCTCAGC ATGCCAGCCT ATGCTCTGCT GGGGAATGTC
    ACCCATGCCA CAGTCACCAT CACAGACGCT GAGGATGAGC CCATGCTGCA GTTCGACAGG
    AAGACGTACC ACGTCAGTGA GAGCGCTGGG TTTCTCTCTG CGCCTGTTGA AAGGAAAGGA
    GATGCTAGCA GCACTGTGTC TGCTGTCTGC TACACTGTCC CCAAATCAGC CAAGGGAAGC
    AGCCTTTATA TGCTGGAGTC TGGCTCCGAC TTCAAGTCCC GAGGGATGAC AGATGACAGC
    CGCATAGTTT TTGGACCAGG TGTTACCATG TCGACATGTG ATGTCATGCT TATTGATGAC
    AGTGAATATG AAGAAGAAGA GGAGTTTGAG ATAGTATTGG CTGATGCTTC GGAAAATGCA
    CGCATTGGCA ACCGGGCTTC AGCCAGCGTC TTCATCGGTG GTCCCAATGA TGCCTCGACA
    GTGTTCCTGG GAAATGCCAC GTTCACCGTC AGTGAGGCTG CAGGAAACAT TGAAATTCCA
    GTCCTCCGTC ACGGACCTGA TCTTTCAACC CCCACCTCAG TGTGGTGTGC TACTCGGACA
    TCAGATCCTC CATCTGCCAG CCCAGGAGTT GATTATGTCC CCAGCTCCAG AAAAATAGAG
    TTCAGGCCAG GCAAGACAGA AGAGTTCTGC ACTCTGACAA TCCTGGATGA TGCTCAGTAC
    CCAGTGATAG AAGGGCTGGA GACATTTGTT GTTTATCTCA GCTCACCCCA TGGAGCTGAG
    CTGATAAAGC CCTTCCAAGC CATTGTGGCC ATTAATGACA TCATCCAAGA TGTACCAACC
    ATGCAGTTCA CCAAGGATGT GTTCACAGTG AAGGAAAAGG AGGGTGCACT ACACATCCCC
    ATTTTCCGCA CTGGCGACCT GAGCTATGAG TCCTCCGTGA GGTGCTACAC CCAGAGCCAG
    ACAGCAGAAG TCATGGAGGA CTTTGCAGAG AGGAGAAATG CAGACGAGTC CCGCATCACT
    TTCTTGAAAG GGGAGAAGGT GAAAAACTGC AGTGTTTACG TCAGTGATGA CTCTGCCTTT
    GAACCAGAGG AGCAGTTTCA TGTCCATCTT GGTAGTCCGC TTGGAAACCA TTGGAGTGGA
    GCTAGGATTG GGAAGATAGA CATGGCAACT GTGACCATCA CTAATGATGA AGACGCACCC
    ACCATTGAGT TTGAAGAAGC TGCGTACCAG GTACGGGAGC CACCTGGACC AGAGGGTGTC
    GCTGCTTTAA ATATCAAAGT GGTCCGGAGA GGGGATCAGA ACAGGACCTC AAAAGTGCGC
    TGTAGCACTC GTGATGGCTC AGCTCAGGCC GGAGTGGATT ACTACCCCAA GAGTCGAGTG
    CTCAAATTCA GCCCAGGTGT GGACCACATC ATGTTTAAGG TGGAGATTAT GTCCAACGAA
    GATCGGGAGT GGCACGAGTC CTTTTCTCTG GTGCTGGGTC CAGATGACCC AGTGGAAGCT
    GTTCTTGGAG ACACCAGCAC TGCAACAGTG ACTATTTTGG ATCAGGAGGC AGCAGGCAGC
    CTGATTCTGC CAGCACCTCC TGTGGTCGTC TCCTTGGCTG ATTATGACCG TGTGGAAGAA
    GCGACCAAGC ACGGTGCTAA GAAATCCCCT TCTCCAGGCT ATCCTCTCAT CTGTGTCACT
    CCCTGTGATC CTCACTTCCC CAAGTTCATG GTCATGAAGG AGCGCTGCAG CGAGGCCGGG
    ATCAACCAGT CTTCCATACA GTTCAGCTGG GAAGTAGCAA CCCCCACAGA TGGGAATGGG
    GCCAGGTCGC CTTTTGAAAC CATCACTGAC AACACACCAT TCACCAGCGT CAACCATAAG
    GTGCTGGACA GCATTTATTT CAGCCGGCGG TTCCACGTGC GCTGTGTGGC CAAGGCTGTG
    GACAAGGCTG GCCATGTGGG CACCCCCTTG AGGAGCAACG CAGTTACTAT AGGGACAGAC
    AGTGCCATTT GTCACACTCC TGTGGTCGCG GGGACAGCTC GAGGCTTCCA GGCACAGTCG
    TTTGTTGCCA CTCTGAAGTA TCTGGACGTC AAACACAAGG AGCATCCCAA CAGGATCCAC
    ATCTCAGTGC AGATCCCCCA CCAGGATGGA ATGCTGCCAC TCATCTCCAC CCTGCCCCTG
    CACAATCTGC ATTTCCTGCT CTCTGAGTCT ATCTACAGAC ACCAGCATGT CTGCTCAAAC
    CTGGTCACCA TCAGAGACCT TAAAGGCATC TCAGAGGCAG GATTCCTTGA TGAAGTGACA
    TATGATAGCA TTGTTCTCGG TCCTGGCTAT GACCGCCCTT ACCAGTTCGA CCCCTCCGTG
    AGAGAGCCAA AGACAATCCA GCTGTACAAG CACCTCAATC TGAAGAGCTG CATATGGACC
    TTTGATGCCT ATTACGACAT GACTGAATTA ATTGATGTCT GTGGAGGCTC TGTAACAGCT
    GACTTCCAGG TACGGGACTC TGCTCAGTCC TTCCTAACAG TCCACGTCCC CCTCTATGTG
    TCCTATATTT ATGTGACAGC GCCGAGAGGC TGGGCTTCTC TTGAGCATCA CACAGAGATG
    GAGTTCTCCT TTTTCTACGA CACTGTTCTC TGGAGGACAG GGATTCAGAC GGACAGTGTC
    CTCTCAGCCC GGCTGCAGAT AATCAGAATC TACATCCGTG AGGATGGCCG ACTGGTTATT
    GAGTTCAAGA CCCAGGCCAA GTTCAGAGGG CTCTTTGTAA TGGAGCACCA CAGCTTGCCA
    GATGTGAAGT CGTCTTTGAT GACTCCAGAC CATCTGGGAG GGATCGAATT TGACCTGCAA
    CTGCTGTGGA GTGCTCAGAC TTTTGACTCT CCCTACCAGC TCTGGAGGGC AACCAGCTCT
    TATAACAGGA AGGACTACTC TGGAGAGTAC ACAATCCTCT TAATTCCTTG TACTGTGCAG
    CCTACACAGC CATGGATAGA GCCTGGAGAG AAGCCCCTGC CCTGTACAGC TCATGCTCCC
    GAGCGATTTT TGATCCCAAT CGCCTTCCAG CAGACAAACC GCCCAGTTCC TGTTGTCTAC
    TCCCTAAACA CGGAGTTTCA GCTGTGTAAC AATGAGAAGG TGTTTCTGAT GGATCCCACC
    AAGTCCGAAA TGTCTCTGGC AGAAATGGAT TACAAAGGGG CTTTTTCAAA AGGGCAAATC
    CTGTATGGCC GTGTGCTCTG GAATCCTGAA CAAAACCTAA ACGCTGCTTA CAAACTGCAG
    CTGGAGAAAG TCTACCTGTG CACTGGCAAG GATGGCTACG TGCCCTTCTT TGACCCAACA
    GGAACTGTCT ATAACGAAGG GCCTCAGTAT GGCTGTATTC AACCCAACAA GCACCTGAAA
    CACAGATTTC TCCTCTTGGA CCGAAACCAA CCAGAAGTCA CAGATAAGTA TTTCCACGAT
    GTGCCTTTTG ATGCCCACTT TGCCTCTGAA CTGTCTGAGT TTCACTCAGT AAGCAGCATG
    CCTGGTGTGG ATGGATTTAC TCTCAAGGTG GATGCTCTCT ACAAGGTGGA AGCTGGACAT
    CAGTGGTACC TTCAAGTTAT CTACATAATA GGCCCAGAGA GCATTGCGGG GCCTCGTGTT
    CAACGATCTC TTACTCACCA CTTAAAGCGT GGCCGCAGGG ACCTGGTTGA CAGTGATGGG
    AGGCTGATGC TGGATGACTC CTTGATCTAC GACAACGAGG GCGATCAGGT CAAGAACGGC
    ACCAACATGA AGTCGCTGAT CTTGGAGATG GAGGAAGCTG TGGTCGCAGC CTCCTTATCA
    CAGACGGGTG CTTCCCTCGG GAGTGCATTT GCTGCTGTCA TGCTGCTGTT GCTGGTGCTT
    CTGGGAGTCT GCCTTGTCAC CAGGAAGTGC AGGACGCTGC GGAGGAAGCG AGAGGCTGTC
    CGAGCTGCCC CAGAGGAACA CCCTCTGAAC ACTAAGGTGG AGCTGCCCAA GGGCACAGAG
    AAGGCAGTGA ACAGCCAGTA CTGCACCGTG AGGAACATCA ATATACTGAG GGAAACAGGG
    GGCATCTCCA GTGTCAAAAG CAGAAAGGTG AAGCAGGTGA ACTTAGAAGT TAAAGTCCAC
    AATAATTTGC ACGACGGAAC AGAAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07890
    Clone ID Related Accession (Same CDS sequence) XM_420470.6 , XM_025150291.1 , XM_420470.5
    Accession Version XM_420470.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11970bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product extracellular matrix protein FRAS1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_420470.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    ATGGGCGCCT GGCTGCCGCT GTGCCTGCTC AGCGCCGCGC TGGCCGCCGC CGCCGCCGCC 
    GCCTCAGGTG CCTGTGTCTA TCAAGGCTTG TTGTTTGAGA ATAACACAAT TTGGAAGCCA
    GATTCTTGCC AAGACTGCAG TTGCCAAAGC AATGTTGTCA CCTGTGAACC TGCTGTCTGC
    GAACATCCTC AGTGTGACTT TGAGAAGGGA GAAGTGCTCC AAATAGCCCC AAACAAATGT
    TGCCCTAAAT GTGCCTCACA AACTGAAGGA TATTGCCAGC ATGAAGGACA GATCCACAGT
    CATGGCACGC AGTGGGCCAG CTCTGGATGT GTTCTGTGCT CCTGTGCCCA TGGGAAGGTG
    AGCTGCACGC CCATAGCCTG CCCAGTCCTC ACCTGTGAAA GGGATGAGCT GCCATACACT
    GCCCAGGGAG CCTGTTGTCC CACATGCGTG GGCCTTGGTG AGCCCTGCTA CTTTGATGGC
    CGTTCGTTCC GGGATGGTGA GGACTGGCGC CTTGGTCGGT GCTCCAAATG TGTCTGCAGG
    GATGGCGTGA CTCAGTGCTT CACAGCTTCC TGTGAGGCTG TTCTGTGCAA ACAGGATGAA
    GTTTTGGCTA TGTCTCCTGG AAAATGCTGT CCAGAATGTG TTCCAAAGTC CTGCTCGGTG
    TCAGGAAAGG TGTACCAGCA CGGAGAACGG TGGAAAAAAA ATGCCTGCAG TGCGTGTGCG
    TGTCATAGAG GGGCAGTCAG ATGTCTGAGA GAAACCTGTG ACTCTGTTAT TTGTGAGAAG
    GGGGATAACA AAGTGCAGCG TTCCGGGAAG TGCTGTGAGG AGTGTGTGTC TTCCAAGGAA
    AACTGCTTGT ATGATGGCAC TATCAGGTAC CACGGTGAGA TGTGGAATAT TACTCGCTGT
    GACTTCTGCA TGTGTGATGA AGGTCAAGTG ACTTGTCACA AAGCAGAGTG CGCCAAAGTA
    GAATGTGCCA AGGGTGAAGA ATTAATTCAC TTAGATGGGA AGTGCTGTCC TGAATGTATT
    TCAAGTAATC GTCATTGTTT TTACAAAGGA CATACCAAAG CTAGCGGAGA GACGTGGAAG
    GAAGGGCTGT GCAGAGAGTG TGAATGCCGC GACTCAGAGG TGGTCTGCTT TCAGCTTTCC
    TGTCCTCCCT GCCCACCGGG CAGCATGGCT GTCAGGGAGA AGGGTGATTG CTGTCCACGC
    TGCCAGCCAG GAAAGTGCCA CCCAGACTGT TTGACCTGCT CGCAGTCCTT TGATCACTGT
    ATTGCCTGCC GTGATGTGAA GAAGCAGCTG CAGAATGGGC GCTGTGTGGA GAGCTGTGGG
    TCAGGCTTCT ATCAGGATGG GGGGACTTGC TTAGCCTGCA ATGAAACATG CTCAGCCTGT
    ACGAATGGTT TTGAGTGTTC CTCATGCCAG ACGTCCCTGC TGATGAAGAA TGGGCAGTGT
    GTGACTTCAT GTGGGAAGGG ATTCTTTCAG GATCAACTCA TATGTGCAGT TTGTCATGAG
    TCCTGTTCCT CGTGTTGGGG ACCTGCTGAC AACAGTTGTC TCTCCTGCAA AGACACGTCA
    CATGTGCTAA AAGCAGGGCT CTGCGTGGCC AGCTGTGGAA AGGGATTTTA TGAGAAGGAT
    GGAATCTGCA GTGTTTGTGA CCCGTCCTGT GAAACGTGCA ATTCAGCCCA GCCCAGTTGT
    TTAACCTGTG CTTCAGATAA GGTGTTACAC AACGGGAACT GCATTTCAGA GTGCCCAGGC
    GGATATTTCC CAGGCAGCAA TGGAAGATGC AGGGCTTGTC ATGATTCGTG CTCCACCTGT
    GAGGGACCTC TGGCCACTCA CTGTACCTCC TGTTCCCTTC CTCTGGCTTT GCACCAGGGC
    CAGTGCCTGC AGGGCTGTGG AGAGGGCTTT TATCAGGATC ACAGCATTTG CAGGGGTTGC
    CACCCATCCT GCCGTAACTG CATTGGCCCA GGGGCCTCTC ATTGCCTGCA GTGCTGGGCA
    CCAGAGGATG TGCTGCAGCC CCATCAACCT GTGGAAGGAG CTGTGCATGG CTTTTGCCTC
    TCACACTGCC AGGCCCAGTT CTACCTGGAT GTCACAGGAG TCTGCAAACA GTGCCACTCC
    TCCTGTGCAG GCTGCATAGG GAACACTTCC CAGGACTGTA CGGCCTGTCC GTCTTCGCAC
    ATGCTGCTCG AGGGCAGATG CCTCTCCGAG TGTCCAGAGG GGTTGTTCAG CCAGCAGGAT
    CACTGCTCCT CCTGCCATCC GTCATGCAAG ACATGCCATG GCCCTTCTGA TTTGGAGTGC
    TTAACCTGCC ATCCCCATGC AACTCTGGCT GCTGGAAGGT GCAGGACTAG CTGCAAGGAA
    GAGCAGTATC TCAACTTGGT TGGATATTGC GTGGACTGCC ATCCTTTGTG CCATCAGTGT
    GTGGCCAACC TGTGGGACAC TGGCAGCCTC TGTCTCAAGT GCCAGAATGC CCGTCACTTG
    CTCCTTGGGG ATCACTGCGT TCCTGACTGT CCAGCAGGAT ACTACACAGA GAGCGGCGCC
    TGCAAACGAT GTCACCCCTC ATGTAAAACC TGCAGAGGTA GGGGTCCTCT TTCCTGCTCC
    TCCTGCAACA CCAGCCTGGT TCTGTCCCAC ACCAGCATGT GCGTTCCAGT CTGTTCTCCG
    GGATACTACA AGGATCAGAG CCAGACCTGC AAACCTTGCA ACAGCCAGTG CCTGAACTGT
    GAATCAGCTA CAGGCTGTAT GTCTTGCAGA GATCCAGCTA AGGTCCTGTT ATTTGGAGAA
    TGCCAGTATG AGAGCTGTGC TCAGCAGTAT TATCTGGATT TTTCAACCAA GACTTGCAAA
    GAGTGTGACT GGAGCTGCAA TGCTTGTAAA GGCCCTCTGA GGACCGATTG CCTGCAGTGT
    ATGGAGGGTA GTGTACTCCA CAAAGGAGCT TGTGTGGAAC AGTGCCCCAC AGCTTTCTAC
    AAGGAGTCAG ACATGTGCCA GAGGTGTGAT CAACATTGCC TTCAGTGTCA CCAAGCAAAT
    GAGTGCAGCC TTTGTGAAGC CCCATTTTTC CTCTTGGACA CTCAGTGCGT TTATAAGTGT
    GGGAAGGGAT ATTATGCAGA CCACGCACAG CGAAAATGCA TAGCTTGTCC TCACGGGTGC
    TTGGAGTGTG ACAGTGACGG CCTTTGCCAC CTCTGTGATG GCAACACCTT TCTGAAAAAC
    AAGATTTGCG TTTCTGCCTG TGGACAGGGA TTCTATGGCA ATAAGTGGAC CAGAGAGTGT
    GAAGTCAGCA GGCATCCTTC AAGCTTCCGT GTAAACAGTA CCCTCACAGT AGGGATTGGT
    GGGGTAAAGC CTCTAGACCT CTCCTTACTG GATGTGCGTG ATCTGGATGG TGACACAGGA
    CAGCTCTTGT TCCATATTGA CAGCGCTCCC TCCAATGGCA GGCTAGTGAT GGTGGTGAAT
    GGGAAAGAAG CGCAGCTGAG CAAAGGTGAC CACTTCAGCT TCAAGGATGT GAAGGAGAAT
    AGAGTCCGCT TTGTGCACAG CAGAGAGAAA TCCAGGAAAG GACAGCTTTC CTTGAGAGTC
    AGCGATCAAC AGTTCTCCCA TCCTGAGGTG ATCAACATTC AAGCATTTTC AACACAGGCC
    CCCTATCTGC TGAGAAATGA GGCCCTCCTT GTCCGCAGAG GGGAAATTGG AACTATAACA
    GACCTGTTGC TTGATGTGAG AGATGGTGAC AATCCTCAGG ATGTCATGTT GATGGTTTTA
    GAGCCTCCAC GTCATGGTCA GCTGATAAAA CCTCCCAGGG ACTCAGCTTC TTCAGTCTAC
    ATGTTCCACC TCGATGACCT TGCTGGTGGA CTGCTACGTT ATGCTCACGA TGGCTCTGAC
    ACCCAGGATG ATGCAATTCT TCTGCAAGTG AACGATGGAT ACCACTTCCA GAACATCCTG
    TTCCACATTA AGATCGCTCC CAAGAGTTAC AGAAGCCCTT GGCTGGTGAC AAATTCCCTG
    GTCTGGGTGC CCCAGGGAGG CATGCTGCAG ATCTCCAAGA CAATTCTGCA CGCTGAGGTA
    CCTGGAGCCC GTGACTCTGA GATAACCTAC AACATCATCA AGGACCAGCC AAGACATGGT
    GAAGTGGTGC TTCTGGTCCC AATGCCGGCA GATGGCCCAG CAGAGTCATG GCAGCTTTTG
    CCTGATGGAA GAGCGGCCTC ACCTACAACC TCTTTTACCC AGCAGGACAT CAATAATGGC
    ATCGTGTGGT ACAGGCACTC TGGAACTCAA GTAGAGAGTG ACTCCTTCCA GTTCCAGGTG
    TCCAGCTCTG CCAGTCCACA AGCCAGCTTG GAAAGCCACG TGTTCAACGT TGCTGTGCTC
    CCACAGACAC CCAGAGCACC TCAGCTTTCC CTTGGCTCCT CTTTGCACAT GGCTGTGCTG
    GAGGACCGCG TGACAGTGAT TGAGCCCCAT CACCTCTCCT TTGTAGACCC AGAGATTCCC
    AGTGAGAAAA TCCTGTTCAA CGTGACTGTC CCTCTGCTTC CTGGCCAAGG TATTGTGGAG
    CACAGAGACA GGCCTCACTC GCCAGTCAGG TATTTCACTC AAGCAGATAT AAACCATGGC
    AAGATTGCTT ATCGTCCACC AACTGCAGCT CCACACTTGA GAGAGATCAT TGCATTCTCA
    TTTGCAGGTC TCCCAGAGTC AGTGAACTTC CGATTCACAG TGTCTGGTGG AGAACACACA
    ACCCCGGAGA TGGCGTTTGT CATTCACTTG CTCTCTGCAG AGCAGCAGCC TCCAGTCTTC
    CAGATCACAG CTCCTTTCCT GGAGGTCAGC CAGGGGGGAA AGGCCACCAT CGGTGTCCAG
    TTGGCTGTGA GTGACACAGA TACAGCTCCT GAGGGCCTCT TCTTTGAGCT GGTGAAACCT
    CCCCATCATG GCATCGTGCT CAAGCATGAT GCAGGTGTGC AGGAGCACAT GAGAGCAGGT
    GACACCTTCA CCTATGAGGA CGTCACTCGA AATGCTCTGC AGTACGTACA TGATGGCTCA
    TCGGCAGCAG AAGACAGCAT GGAAATCTCT GTCACCGATG GCGTCACCAC AGTGACCACA
    GTGCTGAGAG TGGAGGTGTC CCTACTGGAT AACAACGGCC CACAGCTGGC CCCAGGCTGC
    CTGTTGGCAA TCACTGTGGC TAGTAAAAGC TCTGCAACTC TCTCTCGACT GCACCTCGCT
    TACATTGACA ACAATTCTCC TGACTCAGAG ATAAGAATCC AGTTAATATC TCTGCCCACG
    TACGGCACCC TCTTACGGAC GTCTGGCTCT CATGCTGAAG AACTTTCTAG GGTTTATAAT
    TTCACCATGG AAGACATCAA CAACCAGAGG ATCAGTTACT CCACTGTGTT TGAGACAAGC
    AATCAGCCAG TTACGGATGC CTTTCACTTC ACTGTTTTTG ATGCTGATAA TAACCGGCTT
    GACAGACAGA TGTTTACCAT CACCATCACG TCTGCCCAGA CAGCACCATC ACTCATTGTG
    TTTGCTGACC ATATCGCTGT GGATGAGGGG GGCAGGGTCC CACTTCTGGC TCATCACCAC
    TTAACTGCTG ATTATGATAC TGCTGCCAAG GAAGACCTGA GGGTCACGGT TATCACTCTA
    CCCGTGTATG GCTACATTGA AAACACTAAG ACAGGTGAGA CTTTTGGACC ACAGAGTGCT
    GTGACCACAG ATGCATCCTT CTCCATTCAA GATGTTCTAG AGAACAACAT TTACTACTTT
    CAGAGCGTCC ATGAAAGCAT CGAGCCAACA AGTGATGTTT TCAGCTTTTA CGTGAGCGAT
    GGCTTCAGCC AGTCTGAGGT GCACAGCATC AACATCACCA TTCAGCGACG GAATGATGAG
    CCCCCAAAGA TGGTGTTGGA GCCTGTCAGA GTGCATGAGA GCTCAGTGGT TGTTACCAGT
    GCCTCACTCA ACCTGCAGGA CCTGGACACC CAGAACAGTG AGCTTGTCAT CATGGTTACC
    AAAACTCCTG AGCACGGTCA CCTTCGCAGG AGGCAGACTG CTGTGGAGCC CCCAGAGCGT
    GGACACGTGC TGTCTCTGGG CTCATCCTTC ACCTACCAGG ACATTTTGGA TGAGCTGATT
    GTTTATTCTC AAAGCGGTGC AGCAGCACAG ACGGATGAGT TTGGCTTCTC CATCACAGAT
    GGTCTCTACA TGCAGACAGG GAAGCTGGAG TTCTCCATGG AGCTTCCAAA GAAGCAGCTG
    CCCACATTGA CTGCCAGCAG GGTCCTGCAG CTCCCCACTG GCTCTGCAGC ACACATCACA
    GATCAACACT TGAAAGCAGC AGCTGTTTAT CCAGATGACA CAATGATCAG ATTTGTCATG
    AAGAGGGATC CCAGCGTGGG CCATTTGCAG TTGACTGATA TTGATAATCC AGTCGAGATC
    TCTGTCAGAG GGCCTGTCAA AAGTTTTACC CAGGCTGATA TAAATAAAGG GCAAGTGGTT
    TATAGTCACA AGAAGGGGGA TCCTGGTGGA AATTTTGCCT TCACCTTTGA TGTGATCGAT
    GCAGATGGCA GCAGACTGAT GGACCAGTTT TTTCATATCA CTGTATTAGA AGACAGGTTC
    CCCCCCTCCA TCGTCGCTAA CACAGGGCTG GTCTTAGATG AGAACTCTGC AAAGAAGATC
    ACAACACTGC AGCTCTCTGC CACTGACCGG GACAGTGAGC CGACCCAGCT CCAGTACAAG
    CTCACCAAGC AGCCACAGCT GGGGCACCTG GAGCATGTGG CCTCCCCAGG GACAAAAATC
    AGTTCTTTCA GCCAGGCAGA CTTGGCCTCT CGCAGCATCC AATACGTTCA CACTAGCGAC
    ACTGAAAAGC ATACGGATGC CTTCACCTTC TCTGTGTCTG ATGGAACAAA TGAGGTCAGC
    CAGACATTTG ACATCACCAT AAACCCTGTG GATGATTCCT TACCTGTAGT GCAAAGCTTT
    GGGATGAGAG TTCAGGAAGG TGTGAGAAAA ACCATCACAG AGTTTGAGCT TAAAGCAACA
    GATGCTGACA CAAAGGCTGA GGCAATTACA TTCACAGTCG TGCAGCCCCC TCGACATGGC
    CTGATTGAAC ACACCAGCAA TGGGCAGCAT TACCACCAAG CCACCACCTT CACCATGGAT
    GACATTTACC AGAACCGCAT CAGCTACAGT CACGATGGAA GCAACTCACT GAAGGATCGC
    TTTGCTTTCA CTGTGTCTGA TGGGACCAAT CCCTTCTTCA TTATGGAGGA TGGGGGGAAA
    AAGGTTGTGA CAGCAGCACC ACAGCGCTTC AGAATGGACA TCCTCCCTGT GGATGATGGG
    ACACCCAGGG TGGTCACCAA CCAGGGTTTG CAATGGCTGG AGTATGTGGA TGGCAAGGCA
    ACCAATCTAA TCACCAAGAA GGAATTACTG ACAACAGATC CTGACACAGA TGACAAACAG
    CTGATCTATG AGATAACAGA TGGTCCCAGG AATGGTTATG TGGAGAACAA GCTGAAACCA
    GGAACAACTG TGACCATGTT TTCTCAGGAG GATGTGAATC AGGGCCTCAT TCGGTATGTG
    ATGCATGAGG AGAAGGTTCA GGAGACTATG GACAGTTTTC AGTTCCTCGT GAAGGACAGC
    AAACCCAATG TGGTCCGTGG CAACGTCTTC CACATCCAGT GGTCTCTCCT CAGCTTTACA
    TACAGCAGCT ACAACGTCAG TGAGAGCGCT GGCTCTGTGA GCGTCACCGT GCGGCGGATT
    GGTAACCTTA ACCAGTATGC CATTGTCTTG TGCCGCACGG AGCAGGGAAC AGCCACTTCC
    AGCTCTGGTT CACAGTCTGA AGAGCAGGAC TATGTGGAGT ACGCAGGGCA GGTGCAGTTT
    GAGGAGCGGG AAGACACCAA GGCCTGCACC ATCGTCATTA ACGATGACGA CATCTTTGAG
    AGCACCGAGA GCTTCACTGT GGAGCTCAGC ATGCCAGCCT ATGCTCTGCT GGGGAATGTC
    ACCCATGCCA CAGTCACCAT CACAGACGCT GAGGATGAGC CCATGCTGCA GTTCGACAGG
    AAGACGTACC ACGTCAGTGA GAGCGCTGGG TTTCTCTCTG CGCCTGTTGA AAGGAAAGGA
    GATGCTAGCA GCACTGTGTC TGCTGTCTGC TACACTGTCC CCAAATCAGC CAAGGGAAGC
    AGCCTTTATA TGCTGGAGTC TGGCTCCGAC TTCAAGTCCC GAGGGATGAC AGATGACAGC
    CGCATAGTTT TTGGACCAGG TGTTACCATG TCGACATGTG ATGTCATGCT TATTGATGAC
    AGTGAATATG AAGAAGAAGA GGAGTTTGAG ATAGTATTGG CTGATGCTTC GGAAAATGCA
    CGCATTGGCA ACCGGGCTTC AGCCAGCGTC TTCATCGGTG GTCCCAATGA TGCCTCGACA
    GTGTTCCTGG GAAATGCCAC GTTCACCGTC AGTGAGGCTG CAGGAAACAT TGAAATTCCA
    GTCCTCCGTC ACGGACCTGA TCTTTCAACC CCCACCTCAG TGTGGTGTGC TACTCGGACA
    TCAGATCCTC CATCTGCCAG CCCAGGAGTT GATTATGTCC CCAGCTCCAG AAAAATAGAG
    TTCAGGCCAG GCAAGACAGA AGAGTTCTGC ACTCTGACAA TCCTGGATGA TGCTCAGTAC
    CCAGTGATAG AAGGGCTGGA GACATTTGTT GTTTATCTCA GCTCACCCCA TGGAGCTGAG
    CTGATAAAGC CCTTCCAAGC CATTGTGGCC ATTAATGACA TCATCCAAGA TGTACCAACC
    ATGCAGTTCA CCAAGGATGT GTTCACAGTG AAGGAAAAGG AGGGTGCACT ACACATCCCC
    ATTTTCCGCA CTGGCGACCT GAGCTATGAG TCCTCCGTGA GGTGCTACAC CCAGAGCCAG
    ACAGCAGAAG TCATGGAGGA CTTTGCAGAG AGGAGAAATG CAGACGAGTC CCGCATCACT
    TTCTTGAAAG GGGAGAAGGT GAAAAACTGC AGTGTTTACG TCAGTGATGA CTCTGCCTTT
    GAACCAGAGG AGCAGTTTCA TGTCCATCTT GGTAGTCCGC TTGGAAACCA TTGGAGTGGA
    GCTAGGATTG GGAAGATAGA CATGGCAACT GTGACCATCA CTAATGATGA AGACGCACCC
    ACCATTGAGT TTGAAGAAGC TGCGTACCAG GTACGGGAGC CACCTGGACC AGAGGGTGTC
    GCTGCTTTAA ATATCAAAGT GGTCCGGAGA GGGGATCAGA ACAGGACCTC AAAAGTGCGC
    TGTAGCACTC GTGATGGCTC AGCTCAGGCC GGAGTGGATT ACTACCCCAA GAGTCGAGTG
    CTCAAATTCA GCCCAGGTGT GGACCACATC ATGTTTAAGG TGGAGATTAT GTCCAACGAA
    GATCGGGAGT GGCACGAGTC CTTTTCTCTG GTGCTGGGTC CAGATGACCC AGTGGAAGCT
    GTTCTTGGAG ACACCAGCAC TGCAACAGTG ACTATTTTGG ATCAGGAGGC AGCAGGCAGC
    CTGATTCTGC CAGCACCTCC TGTGGTCGTC TCCTTGGCTG ATTATGACCG TGTGGAAGAA
    GCGACCAAGC ACGGTGCTAA GAAATCCCCT TCTCCAGGCT ATCCTCTCAT CTGTGTCACT
    CCCTGTGATC CTCACTTCCC CAAGTTCATG GTCATGAAGG AGCGCTGCAG CGAGGCCGGG
    ATCAACCAGT CTTCCATACA GTTCAGCTGG GAAGTAGCAA CCCCCACAGA TGGGAATGGG
    GCCAGGTCGC CTTTTGAAAC CATCACTGAC AACACACCAT TCACCAGCGT CAACCATAAG
    GTGCTGGACA GCATTTATTT CAGCCGGCGG TTCCACGTGC GCTGTGTGGC CAAGGCTGTG
    GACAAGGCTG GCCATGTGGG CACCCCCTTG AGGAGCAACG CAGTTACTAT AGGGACAGAC
    AGTGCCATTT GTCACACTCC TGTGGTCGCG GGGACAGCTC GAGGCTTCCA GGCACAGTCG
    TTTGTTGCCA CTCTGAAGTA TCTGGACGTC AAACACAAGG AGCATCCCAA CAGGATCCAC
    ATCTCAGTGC AGATCCCCCA CCAGGATGGA ATGCTGCCAC TCATCTCCAC CCTGCCCCTG
    CACAATCTGC ATTTCCTGCT CTCTGAGTCT ATCTACAGAC ACCAGCATGT CTGCTCAAAC
    CTGGTCACCA TCAGAGACCT TAAAGGCATC TCAGAGGCAG GATTCCTTGA TGAAGTGACA
    TATGATAGCA TTGTTCTCGG TCCTGGCTAT GACCGCCCTT ACCAGTTCGA CCCCTCCGTG
    AGAGAGCCAA AGACAATCCA GCTGTACAAG CACCTCAATC TGAAGAGCTG CATATGGACC
    TTTGATGCCT ATTACGACAT GACTGAATTA ATTGATGTCT GTGGAGGCTC TGTAACAGCT
    GACTTCCAGG TACGGGACTC TGCTCAGTCC TTCCTAACAG TCCACGTCCC CCTCTATGTG
    TCCTATATTT ATGTGACAGC GCCGAGAGGC TGGGCTTCTC TTGAGCATCA CACAGAGATG
    GAGTTCTCCT TTTTCTACGA CACTGTTCTC TGGAGGACAG GGATTCAGAC GGACAGTGTC
    CTCTCAGCCC GGCTGCAGAT AATCAGAATC TACATCCGTG AGGATGGCCG ACTGGTTATT
    GAGTTCAAGA CCCAGGCCAA GTTCAGAGGG CTCTTTGTAA TGGAGCACCA CAGCTTGCCA
    GATGTGAAGT CGTCTTTGAT GACTCCAGAC CATCTGGGAG GGATCGAATT TGACCTGCAA
    CTGCTGTGGA GTGCTCAGAC TTTTGACTCT CCCTACCAGC TCTGGAGGGC AACCAGCTCT
    TATAACAGGA AGGACTACTC TGGAGAGTAC ACAATCCTCT TAATTCCTTG TACTGTGCAG
    CCTACACAGC CATGGATAGA GCCTGGAGAG AAGCCCCTGC CCTGTACAGC TCATGCTCCC
    GAGCGATTTT TGATCCCAAT CGCCTTCCAG CAGACAAACC GCCCAGTTCC TGTTGTCTAC
    TCCCTAAACA CGGAGTTTCA GCTGTGTAAC AATGAGAAGG TGTTTCTGAT GGATCCCACC
    AAGTCCGAAA TGTCTCTGGC AGAAATGGAT TACAAAGGGG CTTTTTCAAA AGGGCAAATC
    CTGTATGGCC GTGTGCTCTG GAATCCTGAA CAAAACCTAA ACGCTGCTTA CAAACTGCAG
    CTGGAGAAAG TCTACCTGTG CACTGGCAAG GATGGCTACG TGCCCTTCTT TGACCCAACA
    GGAACTGTCT ATAACGAAGG GCCTCAGTAT GGCTGTATTC AACCCAACAA GCACCTGAAA
    CACAGATTTC TCCTCTTGGA CCGAAACCAA CCAGAAGTCA CAGATAAGTA TTTCCACGAT
    GTGCCTTTTG ATGCCCACTT TGCCTCTGAA CTGTCTGAGT TTCACTCAGT AAGCAGCATG
    CCTGGTGTGG ATGGATTTAC TCTCAAGGTG GATGCTCTCT ACAAGGTGGA AGCTGGACAT
    CAGTGGTACC TTCAAGTTAT CTACATAATA GGCCCAGAGA GCATTGCGGG GCCTCGTGTT
    CAACGATCTC TTACTCACCA CTTAAAGCGT GGCCGCAGGG ACCTGGTTGA CAGTGATGGG
    AGGCTGATGC TGGATGACTC CTTGATCTAC GACAACGAGG GCGATCAGGT CAAGAACGGC
    ACCAACATGA AGTCGCTGAT CTTGGAGATG GAGGAAGCTG TGGTCGCAGC CTCCTTATCA
    CAGACGGGTG CTTCCCTCGG GAGTGCATTT GCTGCTGTCA TGCTGCTGTT GCTGGTGCTT
    CTGGGAGTCT GCCTTGTCAC CAGGAAGTGC AGGACGCTGC GGAGGAAGCG AGAGGCTGTC
    CGAGCTGCCC CAGAGGAACA CCCTCTGAAC ACTAAGGTGG AGCTGCCCAA GGGCACAGAG
    AAGGCAGTGA ACAGCCAGTA CTGCACCGTG AGGAACATCA ATATACTGAG GGAAACAGGG
    GGCATCTCCA GTGTCAAAAG CAGAAAGGTG AAGCAGGTGA ACTTAGAAGT TAAAGTCCAC
    AATAATTTGC ACGACGGAAC AGAAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_420470.4
    CDS136..12105
    Translation

    Target ORF information:

    RefSeq Version XM_420470.5
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_420470.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    ATGGGCGCCT GGCTGCCGCT GTGCCTGCTC AGCGCCGCGC TGGCCGCCGC CGCCGCCGCC 
    GCCTCAGGTG CCTGTGTCTA TCAAGGCTTG TTGTTTGAGA ATAACACAAT TTGGAAGCCA
    GATTCTTGCC AAGACTGCAG TTGCCAAAGC AATGTTGTCA CCTGTGAACC TGCTGTCTGC
    GAACATCCTC AGTGTGACTT TGAGAAGGGA GAAGTGCTCC AAATAGCCCC AAACAAATGT
    TGCCCTAAAT GTGCCTCACA AACTGAAGGA TATTGCCAGC ATGAAGGACA GATCCACAGT
    CATGGCACGC AGTGGGCCAG CTCTGGATGT GTTCTGTGCT CCTGTGCCCA TGGGAAGGTG
    AGCTGCACGC CCATAGCCTG CCCAGTCCTC ACCTGTGAAA GGGATGAGCT GCCATACACT
    GCCCAGGGAG CCTGTTGTCC CACATGCGTG GGCCTTGGTG AGCCCTGCTA CTTTGATGGC
    CGTTCGTTCC GGGATGGTGA GGACTGGCGC CTTGGTCGGT GCTCCAAATG TGTCTGCAGG
    GATGGCGTGA CTCAGTGCTT CACAGCTTCC TGTGAGGCTG TTCTGTGCAA ACAGGATGAA
    GTTTTGGCTA TGTCTCCTGG AAAATGCTGT CCAGAATGTG TTCCAAAGTC CTGCTCGGTG
    TCAGGAAAGG TGTACCAGCA CGGAGAACGG TGGAAAAAAA ATGCCTGCAG TGCGTGTGCG
    TGTCATAGAG GGGCAGTCAG ATGTCTGAGA GAAACCTGTG ACTCTGTTAT TTGTGAGAAG
    GGGGATAACA AAGTGCAGCG TTCCGGGAAG TGCTGTGAGG AGTGTGTGTC TTCCAAGGAA
    AACTGCTTGT ATGATGGCAC TATCAGGTAC CACGGTGAGA TGTGGAATAT TACTCGCTGT
    GACTTCTGCA TGTGTGATGA AGGTCAAGTG ACTTGTCACA AAGCAGAGTG CGCCAAAGTA
    GAATGTGCCA AGGGTGAAGA ATTAATTCAC TTAGATGGGA AGTGCTGTCC TGAATGTATT
    TCAAGTAATC GTCATTGTTT TTACAAAGGA CATACCAAAG CTAGCGGAGA GACGTGGAAG
    GAAGGGCTGT GCAGAGAGTG TGAATGCCGC GACTCAGAGG TGGTCTGCTT TCAGCTTTCC
    TGTCCTCCCT GCCCACCGGG CAGCATGGCT GTCAGGGAGA AGGGTGATTG CTGTCCACGC
    TGCCAGCCAG GAAAGTGCCA CCCAGACTGT TTGACCTGCT CGCAGTCCTT TGATCACTGT
    ATTGCCTGCC GTGATGTGAA GAAGCAGCTG CAGAATGGGC GCTGTGTGGA GAGCTGTGGG
    TCAGGCTTCT ATCAGGATGG GGGGACTTGC TTAGCCTGCA ATGAAACATG CTCAGCCTGT
    ACGAATGGTT TTGAGTGTTC CTCATGCCAG ACGTCCCTGC TGATGAAGAA TGGGCAGTGT
    GTGACTTCAT GTGGGAAGGG ATTCTTTCAG GATCAACTCA TATGTGCAGT TTGTCATGAG
    TCCTGTTCCT CGTGTTGGGG ACCTGCTGAC AACAGTTGTC TCTCCTGCAA AGACACGTCA
    CATGTGCTAA AAGCAGGGCT CTGCGTGGCC AGCTGTGGAA AGGGATTTTA TGAGAAGGAT
    GGAATCTGCA GTGTTTGTGA CCCGTCCTGT GAAACGTGCA ATTCAGCCCA GCCCAGTTGT
    TTAACCTGTG CTTCAGATAA GGTGTTACAC AACGGGAACT GCATTTCAGA GTGCCCAGGC
    GGATATTTCC CAGGCAGCAA TGGAAGATGC AGGGCTTGTC ATGATTCGTG CTCCACCTGT
    GAGGGACCTC TGGCCACTCA CTGTACCTCC TGTTCCCTTC CTCTGGCTTT GCACCAGGGC
    CAGTGCCTGC AGGGCTGTGG AGAGGGCTTT TATCAGGATC ACAGCATTTG CAGGGGTTGC
    CACCCATCCT GCCGTAACTG CATTGGCCCA GGGGCCTCTC ATTGCCTGCA GTGCTGGGCA
    CCAGAGGATG TGCTGCAGCC CCATCAACCT GTGGAAGGAG CTGTGCATGG CTTTTGCCTC
    TCACACTGCC AGGCCCAGTT CTACCTGGAT GTCACAGGAG TCTGCAAACA GTGCCACTCC
    TCCTGTGCAG GCTGCATAGG GAACACTTCC CAGGACTGTA CGGCCTGTCC GTCTTCGCAC
    ATGCTGCTCG AGGGCAGATG CCTCTCCGAG TGTCCAGAGG GGTTGTTCAG CCAGCAGGAT
    CACTGCTCCT CCTGCCATCC GTCATGCAAG ACATGCCATG GCCCTTCTGA TTTGGAGTGC
    TTAACCTGCC ATCCCCATGC AACTCTGGCT GCTGGAAGGT GCAGGACTAG CTGCAAGGAA
    GAGCAGTATC TCAACTTGGT TGGATATTGC GTGGACTGCC ATCCTTTGTG CCATCAGTGT
    GTGGCCAACC TGTGGGACAC TGGCAGCCTC TGTCTCAAGT GCCAGAATGC CCGTCACTTG
    CTCCTTGGGG ATCACTGCGT TCCTGACTGT CCAGCAGGAT ACTACACAGA GAGCGGCGCC
    TGCAAACGAT GTCACCCCTC ATGTAAAACC TGCAGAGGTA GGGGTCCTCT TTCCTGCTCC
    TCCTGCAACA CCAGCCTGGT TCTGTCCCAC ACCAGCATGT GCGTTCCAGT CTGTTCTCCG
    GGATACTACA AGGATCAGAG CCAGACCTGC AAACCTTGCA ACAGCCAGTG CCTGAACTGT
    GAATCAGCTA CAGGCTGTAT GTCTTGCAGA GATCCAGCTA AGGTCCTGTT ATTTGGAGAA
    TGCCAGTATG AGAGCTGTGC TCAGCAGTAT TATCTGGATT TTTCAACCAA GACTTGCAAA
    GAGTGTGACT GGAGCTGCAA TGCTTGTAAA GGCCCTCTGA GGACCGATTG CCTGCAGTGT
    ATGGAGGGTA GTGTACTCCA CAAAGGAGCT TGTGTGGAAC AGTGCCCCAC AGCTTTCTAC
    AAGGAGTCAG ACATGTGCCA GAGGTGTGAT CAACATTGCC TTCAGTGTCA CCAAGCAAAT
    GAGTGCAGCC TTTGTGAAGC CCCATTTTTC CTCTTGGACA CTCAGTGCGT TTATAAGTGT
    GGGAAGGGAT ATTATGCAGA CCACGCACAG CGAAAATGCA TAGCTTGTCC TCACGGGTGC
    TTGGAGTGTG ACAGTGACGG CCTTTGCCAC CTCTGTGATG GCAACACCTT TCTGAAAAAC
    AAGATTTGCG TTTCTGCCTG TGGACAGGGA TTCTATGGCA ATAAGTGGAC CAGAGAGTGT
    GAAGTCAGCA GGCATCCTTC AAGCTTCCGT GTAAACAGTA CCCTCACAGT AGGGATTGGT
    GGGGTAAAGC CTCTAGACCT CTCCTTACTG GATGTGCGTG ATCTGGATGG TGACACAGGA
    CAGCTCTTGT TCCATATTGA CAGCGCTCCC TCCAATGGCA GGCTAGTGAT GGTGGTGAAT
    GGGAAAGAAG CGCAGCTGAG CAAAGGTGAC CACTTCAGCT TCAAGGATGT GAAGGAGAAT
    AGAGTCCGCT TTGTGCACAG CAGAGAGAAA TCCAGGAAAG GACAGCTTTC CTTGAGAGTC
    AGCGATCAAC AGTTCTCCCA TCCTGAGGTG ATCAACATTC AAGCATTTTC AACACAGGCC
    CCCTATCTGC TGAGAAATGA GGCCCTCCTT GTCCGCAGAG GGGAAATTGG AACTATAACA
    GACCTGTTGC TTGATGTGAG AGATGGTGAC AATCCTCAGG ATGTCATGTT GATGGTTTTA
    GAGCCTCCAC GTCATGGTCA GCTGATAAAA CCTCCCAGGG ACTCAGCTTC TTCAGTCTAC
    ATGTTCCACC TCGATGACCT TGCTGGTGGA CTGCTACGTT ATGCTCACGA TGGCTCTGAC
    ACCCAGGATG ATGCAATTCT TCTGCAAGTG AACGATGGAT ACCACTTCCA GAACATCCTG
    TTCCACATTA AGATCGCTCC CAAGAGTTAC AGAAGCCCTT GGCTGGTGAC AAATTCCCTG
    GTCTGGGTGC CCCAGGGAGG CATGCTGCAG ATCTCCAAGA CAATTCTGCA CGCTGAGGTA
    CCTGGAGCCC GTGACTCTGA GATAACCTAC AACATCATCA AGGACCAGCC AAGACATGGT
    GAAGTGGTGC TTCTGGTCCC AATGCCGGCA GATGGCCCAG CAGAGTCATG GCAGCTTTTG
    CCTGATGGAA GAGCGGCCTC ACCTACAACC TCTTTTACCC AGCAGGACAT CAATAATGGC
    ATCGTGTGGT ACAGGCACTC TGGAACTCAA GTAGAGAGTG ACTCCTTCCA GTTCCAGGTG
    TCCAGCTCTG CCAGTCCACA AGCCAGCTTG GAAAGCCACG TGTTCAACGT TGCTGTGCTC
    CCACAGACAC CCAGAGCACC TCAGCTTTCC CTTGGCTCCT CTTTGCACAT GGCTGTGCTG
    GAGGACCGCG TGACAGTGAT TGAGCCCCAT CACCTCTCCT TTGTAGACCC AGAGATTCCC
    AGTGAGAAAA TCCTGTTCAA CGTGACTGTC CCTCTGCTTC CTGGCCAAGG TATTGTGGAG
    CACAGAGACA GGCCTCACTC GCCAGTCAGG TATTTCACTC AAGCAGATAT AAACCATGGC
    AAGATTGCTT ATCGTCCACC AACTGCAGCT CCACACTTGA GAGAGATCAT TGCATTCTCA
    TTTGCAGGTC TCCCAGAGTC AGTGAACTTC CGATTCACAG TGTCTGGTGG AGAACACACA
    ACCCCGGAGA TGGCGTTTGT CATTCACTTG CTCTCTGCAG AGCAGCAGCC TCCAGTCTTC
    CAGATCACAG CTCCTTTCCT GGAGGTCAGC CAGGGGGGAA AGGCCACCAT CGGTGTCCAG
    TTGGCTGTGA GTGACACAGA TACAGCTCCT GAGGGCCTCT TCTTTGAGCT GGTGAAACCT
    CCCCATCATG GCATCGTGCT CAAGCATGAT GCAGGTGTGC AGGAGCACAT GAGAGCAGGT
    GACACCTTCA CCTATGAGGA CGTCACTCGA AATGCTCTGC AGTACGTACA TGATGGCTCA
    TCGGCAGCAG AAGACAGCAT GGAAATCTCT GTCACCGATG GCGTCACCAC AGTGACCACA
    GTGCTGAGAG TGGAGGTGTC CCTACTGGAT AACAACGGCC CACAGCTGGC CCCAGGCTGC
    CTGTTGGCAA TCACTGTGGC TAGTAAAAGC TCTGCAACTC TCTCTCGACT GCACCTCGCT
    TACATTGACA ACAATTCTCC TGACTCAGAG ATAAGAATCC AGTTAATATC TCTGCCCACG
    TACGGCACCC TCTTACGGAC GTCTGGCTCT CATGCTGAAG AACTTTCTAG GGTTTATAAT
    TTCACCATGG AAGACATCAA CAACCAGAGG ATCAGTTACT CCACTGTGTT TGAGACAAGC
    AATCAGCCAG TTACGGATGC CTTTCACTTC ACTGTTTTTG ATGCTGATAA TAACCGGCTT
    GACAGACAGA TGTTTACCAT CACCATCACG TCTGCCCAGA CAGCACCATC ACTCATTGTG
    TTTGCTGACC ATATCGCTGT GGATGAGGGG GGCAGGGTCC CACTTCTGGC TCATCACCAC
    TTAACTGCTG ATTATGATAC TGCTGCCAAG GAAGACCTGA GGGTCACGGT TATCACTCTA
    CCCGTGTATG GCTACATTGA AAACACTAAG ACAGGTGAGA CTTTTGGACC ACAGAGTGCT
    GTGACCACAG ATGCATCCTT CTCCATTCAA GATGTTCTAG AGAACAACAT TTACTACTTT
    CAGAGCGTCC ATGAAAGCAT CGAGCCAACA AGTGATGTTT TCAGCTTTTA CGTGAGCGAT
    GGCTTCAGCC AGTCTGAGGT GCACAGCATC AACATCACCA TTCAGCGACG GAATGATGAG
    CCCCCAAAGA TGGTGTTGGA GCCTGTCAGA GTGCATGAGA GCTCAGTGGT TGTTACCAGT
    GCCTCACTCA ACCTGCAGGA CCTGGACACC CAGAACAGTG AGCTTGTCAT CATGGTTACC
    AAAACTCCTG AGCACGGTCA CCTTCGCAGG AGGCAGACTG CTGTGGAGCC CCCAGAGCGT
    GGACACGTGC TGTCTCTGGG CTCATCCTTC ACCTACCAGG ACATTTTGGA TGAGCTGATT
    GTTTATTCTC AAAGCGGTGC AGCAGCACAG ACGGATGAGT TTGGCTTCTC CATCACAGAT
    GGTCTCTACA TGCAGACAGG GAAGCTGGAG TTCTCCATGG AGCTTCCAAA GAAGCAGCTG
    CCCACATTGA CTGCCAGCAG GGTCCTGCAG CTCCCCACTG GCTCTGCAGC ACACATCACA
    GATCAACACT TGAAAGCAGC AGCTGTTTAT CCAGATGACA CAATGATCAG ATTTGTCATG
    AAGAGGGATC CCAGCGTGGG CCATTTGCAG TTGACTGATA TTGATAATCC AGTCGAGATC
    TCTGTCAGAG GGCCTGTCAA AAGTTTTACC CAGGCTGATA TAAATAAAGG GCAAGTGGTT
    TATAGTCACA AGAAGGGGGA TCCTGGTGGA AATTTTGCCT TCACCTTTGA TGTGATCGAT
    GCAGATGGCA GCAGACTGAT GGACCAGTTT TTTCATATCA CTGTATTAGA AGACAGGTTC
    CCCCCCTCCA TCGTCGCTAA CACAGGGCTG GTCTTAGATG AGAACTCTGC AAAGAAGATC
    ACAACACTGC AGCTCTCTGC CACTGACCGG GACAGTGAGC CGACCCAGCT CCAGTACAAG
    CTCACCAAGC AGCCACAGCT GGGGCACCTG GAGCATGTGG CCTCCCCAGG GACAAAAATC
    AGTTCTTTCA GCCAGGCAGA CTTGGCCTCT CGCAGCATCC AATACGTTCA CACTAGCGAC
    ACTGAAAAGC ATACGGATGC CTTCACCTTC TCTGTGTCTG ATGGAACAAA TGAGGTCAGC
    CAGACATTTG ACATCACCAT AAACCCTGTG GATGATTCCT TACCTGTAGT GCAAAGCTTT
    GGGATGAGAG TTCAGGAAGG TGTGAGAAAA ACCATCACAG AGTTTGAGCT TAAAGCAACA
    GATGCTGACA CAAAGGCTGA GGCAATTACA TTCACAGTCG TGCAGCCCCC TCGACATGGC
    CTGATTGAAC ACACCAGCAA TGGGCAGCAT TACCACCAAG CCACCACCTT CACCATGGAT
    GACATTTACC AGAACCGCAT CAGCTACAGT CACGATGGAA GCAACTCACT GAAGGATCGC
    TTTGCTTTCA CTGTGTCTGA TGGGACCAAT CCCTTCTTCA TTATGGAGGA TGGGGGGAAA
    AAGGTTGTGA CAGCAGCACC ACAGCGCTTC AGAATGGACA TCCTCCCTGT GGATGATGGG
    ACACCCAGGG TGGTCACCAA CCAGGGTTTG CAATGGCTGG AGTATGTGGA TGGCAAGGCA
    ACCAATCTAA TCACCAAGAA GGAATTACTG ACAACAGATC CTGACACAGA TGACAAACAG
    CTGATCTATG AGATAACAGA TGGTCCCAGG AATGGTTATG TGGAGAACAA GCTGAAACCA
    GGAACAACTG TGACCATGTT TTCTCAGGAG GATGTGAATC AGGGCCTCAT TCGGTATGTG
    ATGCATGAGG AGAAGGTTCA GGAGACTATG GACAGTTTTC AGTTCCTCGT GAAGGACAGC
    AAACCCAATG TGGTCCGTGG CAACGTCTTC CACATCCAGT GGTCTCTCCT CAGCTTTACA
    TACAGCAGCT ACAACGTCAG TGAGAGCGCT GGCTCTGTGA GCGTCACCGT GCGGCGGATT
    GGTAACCTTA ACCAGTATGC CATTGTCTTG TGCCGCACGG AGCAGGGAAC AGCCACTTCC
    AGCTCTGGTT CACAGTCTGA AGAGCAGGAC TATGTGGAGT ACGCAGGGCA GGTGCAGTTT
    GAGGAGCGGG AAGACACCAA GGCCTGCACC ATCGTCATTA ACGATGACGA CATCTTTGAG
    AGCACCGAGA GCTTCACTGT GGAGCTCAGC ATGCCAGCCT ATGCTCTGCT GGGGAATGTC
    ACCCATGCCA CAGTCACCAT CACAGACGCT GAGGATGAGC CCATGCTGCA GTTCGACAGG
    AAGACGTACC ACGTCAGTGA GAGCGCTGGG TTTCTCTCTG CGCCTGTTGA AAGGAAAGGA
    GATGCTAGCA GCACTGTGTC TGCTGTCTGC TACACTGTCC CCAAATCAGC CAAGGGAAGC
    AGCCTTTATA TGCTGGAGTC TGGCTCCGAC TTCAAGTCCC GAGGGATGAC AGATGACAGC
    CGCATAGTTT TTGGACCAGG TGTTACCATG TCGACATGTG ATGTCATGCT TATTGATGAC
    AGTGAATATG AAGAAGAAGA GGAGTTTGAG ATAGTATTGG CTGATGCTTC GGAAAATGCA
    CGCATTGGCA ACCGGGCTTC AGCCAGCGTC TTCATCGGTG GTCCCAATGA TGCCTCGACA
    GTGTTCCTGG GAAATGCCAC GTTCACCGTC AGTGAGGCTG CAGGAAACAT TGAAATTCCA
    GTCCTCCGTC ACGGACCTGA TCTTTCAACC CCCACCTCAG TGTGGTGTGC TACTCGGACA
    TCAGATCCTC CATCTGCCAG CCCAGGAGTT GATTATGTCC CCAGCTCCAG AAAAATAGAG
    TTCAGGCCAG GCAAGACAGA AGAGTTCTGC ACTCTGACAA TCCTGGATGA TGCTCAGTAC
    CCAGTGATAG AAGGGCTGGA GACATTTGTT GTTTATCTCA GCTCACCCCA TGGAGCTGAG
    CTGATAAAGC CCTTCCAAGC CATTGTGGCC ATTAATGACA TCATCCAAGA TGTACCAACC
    ATGCAGTTCA CCAAGGATGT GTTCACAGTG AAGGAAAAGG AGGGTGCACT ACACATCCCC
    ATTTTCCGCA CTGGCGACCT GAGCTATGAG TCCTCCGTGA GGTGCTACAC CCAGAGCCAG
    ACAGCAGAAG TCATGGAGGA CTTTGCAGAG AGGAGAAATG CAGACGAGTC CCGCATCACT
    TTCTTGAAAG GGGAGAAGGT GAAAAACTGC AGTGTTTACG TCAGTGATGA CTCTGCCTTT
    GAACCAGAGG AGCAGTTTCA TGTCCATCTT GGTAGTCCGC TTGGAAACCA TTGGAGTGGA
    GCTAGGATTG GGAAGATAGA CATGGCAACT GTGACCATCA CTAATGATGA AGACGCACCC
    ACCATTGAGT TTGAAGAAGC TGCGTACCAG GTACGGGAGC CACCTGGACC AGAGGGTGTC
    GCTGCTTTAA ATATCAAAGT GGTCCGGAGA GGGGATCAGA ACAGGACCTC AAAAGTGCGC
    TGTAGCACTC GTGATGGCTC AGCTCAGGCC GGAGTGGATT ACTACCCCAA GAGTCGAGTG
    CTCAAATTCA GCCCAGGTGT GGACCACATC ATGTTTAAGG TGGAGATTAT GTCCAACGAA
    GATCGGGAGT GGCACGAGTC CTTTTCTCTG GTGCTGGGTC CAGATGACCC AGTGGAAGCT
    GTTCTTGGAG ACACCAGCAC TGCAACAGTG ACTATTTTGG ATCAGGAGGC AGCAGGCAGC
    CTGATTCTGC CAGCACCTCC TGTGGTCGTC TCCTTGGCTG ATTATGACCG TGTGGAAGAA
    GCGACCAAGC ACGGTGCTAA GAAATCCCCT TCTCCAGGCT ATCCTCTCAT CTGTGTCACT
    CCCTGTGATC CTCACTTCCC CAAGTTCATG GTCATGAAGG AGCGCTGCAG CGAGGCCGGG
    ATCAACCAGT CTTCCATACA GTTCAGCTGG GAAGTAGCAA CCCCCACAGA TGGGAATGGG
    GCCAGGTCGC CTTTTGAAAC CATCACTGAC AACACACCAT TCACCAGCGT CAACCATAAG
    GTGCTGGACA GCATTTATTT CAGCCGGCGG TTCCACGTGC GCTGTGTGGC CAAGGCTGTG
    GACAAGGCTG GCCATGTGGG CACCCCCTTG AGGAGCAACG CAGTTACTAT AGGGACAGAC
    AGTGCCATTT GTCACACTCC TGTGGTCGCG GGGACAGCTC GAGGCTTCCA GGCACAGTCG
    TTTGTTGCCA CTCTGAAGTA TCTGGACGTC AAACACAAGG AGCATCCCAA CAGGATCCAC
    ATCTCAGTGC AGATCCCCCA CCAGGATGGA ATGCTGCCAC TCATCTCCAC CCTGCCCCTG
    CACAATCTGC ATTTCCTGCT CTCTGAGTCT ATCTACAGAC ACCAGCATGT CTGCTCAAAC
    CTGGTCACCA TCAGAGACCT TAAAGGCATC TCAGAGGCAG GATTCCTTGA TGAAGTGACA
    TATGATAGCA TTGTTCTCGG TCCTGGCTAT GACCGCCCTT ACCAGTTCGA CCCCTCCGTG
    AGAGAGCCAA AGACAATCCA GCTGTACAAG CACCTCAATC TGAAGAGCTG CATATGGACC
    TTTGATGCCT ATTACGACAT GACTGAATTA ATTGATGTCT GTGGAGGCTC TGTAACAGCT
    GACTTCCAGG TACGGGACTC TGCTCAGTCC TTCCTAACAG TCCACGTCCC CCTCTATGTG
    TCCTATATTT ATGTGACAGC GCCGAGAGGC TGGGCTTCTC TTGAGCATCA CACAGAGATG
    GAGTTCTCCT TTTTCTACGA CACTGTTCTC TGGAGGACAG GGATTCAGAC GGACAGTGTC
    CTCTCAGCCC GGCTGCAGAT AATCAGAATC TACATCCGTG AGGATGGCCG ACTGGTTATT
    GAGTTCAAGA CCCAGGCCAA GTTCAGAGGG CTCTTTGTAA TGGAGCACCA CAGCTTGCCA
    GATGTGAAGT CGTCTTTGAT GACTCCAGAC CATCTGGGAG GGATCGAATT TGACCTGCAA
    CTGCTGTGGA GTGCTCAGAC TTTTGACTCT CCCTACCAGC TCTGGAGGGC AACCAGCTCT
    TATAACAGGA AGGACTACTC TGGAGAGTAC ACAATCCTCT TAATTCCTTG TACTGTGCAG
    CCTACACAGC CATGGATAGA GCCTGGAGAG AAGCCCCTGC CCTGTACAGC TCATGCTCCC
    GAGCGATTTT TGATCCCAAT CGCCTTCCAG CAGACAAACC GCCCAGTTCC TGTTGTCTAC
    TCCCTAAACA CGGAGTTTCA GCTGTGTAAC AATGAGAAGG TGTTTCTGAT GGATCCCACC
    AAGTCCGAAA TGTCTCTGGC AGAAATGGAT TACAAAGGGG CTTTTTCAAA AGGGCAAATC
    CTGTATGGCC GTGTGCTCTG GAATCCTGAA CAAAACCTAA ACGCTGCTTA CAAACTGCAG
    CTGGAGAAAG TCTACCTGTG CACTGGCAAG GATGGCTACG TGCCCTTCTT TGACCCAACA
    GGAACTGTCT ATAACGAAGG GCCTCAGTAT GGCTGTATTC AACCCAACAA GCACCTGAAA
    CACAGATTTC TCCTCTTGGA CCGAAACCAA CCAGAAGTCA CAGATAAGTA TTTCCACGAT
    GTGCCTTTTG ATGCCCACTT TGCCTCTGAA CTGTCTGAGT TTCACTCAGT AAGCAGCATG
    CCTGGTGTGG ATGGATTTAC TCTCAAGGTG GATGCTCTCT ACAAGGTGGA AGCTGGACAT
    CAGTGGTACC TTCAAGTTAT CTACATAATA GGCCCAGAGA GCATTGCGGG GCCTCGTGTT
    CAACGATCTC TTACTCACCA CTTAAAGCGT GGCCGCAGGG ACCTGGTTGA CAGTGATGGG
    AGGCTGATGC TGGATGACTC CTTGATCTAC GACAACGAGG GCGATCAGGT CAAGAACGGC
    ACCAACATGA AGTCGCTGAT CTTGGAGATG GAGGAAGCTG TGGTCGCAGC CTCCTTATCA
    CAGACGGGTG CTTCCCTCGG GAGTGCATTT GCTGCTGTCA TGCTGCTGTT GCTGGTGCTT
    CTGGGAGTCT GCCTTGTCAC CAGGAAGTGC AGGACGCTGC GGAGGAAGCG AGAGGCTGTC
    CGAGCTGCCC CAGAGGAACA CCCTCTGAAC ACTAAGGTGG AGCTGCCCAA GGGCACAGAG
    AAGGCAGTGA ACAGCCAGTA CTGCACCGTG AGGAACATCA ATATACTGAG GGAAACAGGG
    GGCATCTCCA GTGTCAAAAG CAGAAAGGTG AAGCAGGTGA ACTTAGAAGT TAAAGTCCAC
    AATAATTTGC ACGACGGAAC AGAAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa150560
    Clone ID Related Accession (Same CDS sequence) XM_025150292.1
    Accession Version XM_025150292.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11829bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product extracellular matrix protein FRAS1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    ATGGGCGCCT GGCTGCCGCT GTGCCTGCTC AGCGCCGCGC TGGCCGCCGC CGCCGCCGCC 
    GCCTCAGGTG CCTGTGTCTA TCAAGGCTTG TTGTTTGAGA ATAACACAAT TTGGAAGCCA
    GATTCTTGCC AAGACTGCAG TTGCCAAAGC AATGTTGTCA CCTGTGAACC TGCTGTCTGC
    GAACATCCTC AGTGTGACTT TGAGAAGGGA GAAGTGCTCC AAATAGCCCC AAACAAATGT
    TGCCCTAAAT GTGCCTCACA AACTGAAGGA TATTGCCAGC ATGAAGGACA GATCCACAGT
    CATGGCACGC AGTGGGCCAG CTCTGGATGT GTTCTGTGCT CCTGTGCCCA TGGGAAGGTG
    AGCTGCACGC CCATAGCCTG CCCAGTCCTC ACCTGTGAAA GGGATGAGCT GCCATACACT
    GCCCAGGGAG CCTGTTGTCC CACATGCGTG GGCCTTGGTG AGCCCTGCTA CTTTGATGGC
    CGTTCGTTCC GGGATGGTGA GGACTGGCGC CTTGGTCGGT GCTCCAAATG TGTCTGCAGG
    GATGGCGTGA CTCAGTGCTT CACAGCTTCC TGTGAGGCTG TTCTGTGCAA ACAGGATGAA
    GTTTTGGCTA TGTCTCCTGG AAAATGCTGT CCAGAATGTG TTCCAAAGTC CTGCTCGGTG
    TCAGGAAAGG TGTACCAGCA CGGAGAACGG TGGAAAAAAA ATGCCTGCAG TGCGTGTGCG
    TGTCATAGAG GGGCAGTCAG ATGTCTGAGA GAAACCTGTG ACTCTGTTAT TTGTGAGAAG
    GGGGATAACA AAGTGCAGCG TTCCGGGAAG TGCTGTGAGG AGTGTGTGTC TTCCAAGGAA
    AACTGCTTGT ATGATGGCAC TATCAGGTAC CACGGTGAGA TGTGGAATAT TACTCGCTGT
    GACTTCTGCA TGTGTGATGA AGGTCAAGTG ACTTGTCACA AAGCAGAGTG CGCCAAAGTA
    GAATGTGCCA AGGGTGAAGA ATTAATTCAC TTAGATGGGA AGTGCTGTCC TGAATGTATT
    TCAAGTAATC GTCATTGTTT TTACAAAGGA CATACCAAAG CTAGCGGAGA GACGTGGAAG
    GAAGGGCTGT GCAGAGAGTG TGAATGCCGC GACTCAGAGG TGGTCTGCTT TCAGCTTTCC
    TGTCCTCCCT GCCCACCGGG CAGCATGGCT GTCAGGGAGA AGGGTGATTG CTGTCCACGC
    TGCCAGCCAG GAAAGTGCCA CCCAGACTGT TTGACCTGCT CGCAGTCCTT TGATCACTGT
    ATTGCCTGCC GTGATGTGAA GAAGCAGCTG CAGAATGGGC GCTGTGTGGA GAGCTGTGGG
    TCAGGCTTCT ATCAGGATGG GGGGACTTGC TTAGCCTGCA ATGAAACATG CTCAGCCTGT
    ACGAATGGTT TTGAGTGTTC CTCATGCCAG ACGTCCCTGC TGATGAAGAA TGGGCAGTGT
    GTGACTTCAT GTGGGAAGGG ATTCTTTCAG GATCAACTCA TATGTGCAGT TTGTCATGAG
    TCCTGTTCCT CGTGTTGGGG ACCTGCTGAC AACAGTTGTC TCTCCTGCAA AGACACGTCA
    CATGTGCTAA AAGCAGGGCT CTGCGTGGCC AGCTGTGGAA AGGGATTTTA TGAGAAGGAT
    GGAATCTGCA GTGTTTGTGA CCCGTCCTGT GAAACGTGCA ATTCAGCCCA GCCCAGTTGT
    TTAACCTGTG CTTCAGATAA GGTGTTACAC AACGGGAACT GCATTTCAGA GTGCCCAGGC
    GGATATTTCC CAGGCAGCAA TGGAAGATGC AGGGCTTGTC ATGATTCGTG CTCCACCTGT
    GAGGGACCTC TGGCCACTCA CTGTACCTCC TGTTCCCTTC CTCTGGCTTT GCACCAGGGC
    CAGTGCCTGC AGGGCTGTGG AGAGGGCTTT TATCAGGATC ACAGCATTTG CAGGGGTTGC
    CACCCATCCT GCCGTAACTG CATTGGCCCA GGGGCCTCTC ATTGCCTGCA GTGCTGGGCA
    CCAGAGGATG TGCTGCAGCC CCATCAACCT GTGGAAGGAG CTGTGCATGG CTTTTGCCTC
    TCACACTGCC AGGCCCAGTT CTACCTGGAT GTCACAGGAG TCTGCAAACA GTGCCACTCC
    TCCTGTGCAG GCTGCATAGG GAACACTTCC CAGGACTGTA CGGCCTGTCC GTCTTCGCAC
    ATGCTGCTCG AGGGCAGATG CCTCTCCGAG TGTCCAGAGG GGTTGTTCAG CCAGCAGGAT
    CACTGCTCCT CCTGCCATCC GTCATGCAAG ACATGCCATG GCCCTTCTGA TTTGGAGTGC
    TTAACCTGCC ATCCCCATGC AACTCTGGCT GCTGGAAGGT GCAGGACTAG CTGCAAGGAA
    GAGCAGTATC TCAACTTGGT TGGATATTGC GTGGACTGCC ATCCTTTGTG CCATCAGTGT
    GTGGCCAACC TGTGGGACAC TGGCAGCCTC TGTCTCAAGT GCCAGAATGC CCGTCACTTG
    CTCCTTGGGG ATCACTGCGT TCCTGACTGT CCAGCAGGAT ACTACACAGA GAGCGGCGCC
    TGCAAACGAT GTCACCCCTC ATGTAAAACC TGCAGAGGTA GGGGTCCTCT TTCCTGCTCC
    TCCTGCAACA CCAGCCTGGT TCTGTCCCAC ACCAGCATGT GCGTTCCAGT CTGTTCTCCG
    GGATACTACA AGGATCAGAG CCAGACCTGC AAACCTTGCA ACAGCCAGTG CCTGAACTGT
    GAATCAGCTA CAGGCTGTAT GTCTTGCAGA GATCCAGCTA AGGTCCTGTT ATTTGGAGAA
    TGCCAGTATG AGAGCTGTGC TCAGCAGTAT TATCTGGATT TTTCAACCAA GACTTGCAAA
    GGGTGTGATC AACATTGCCT TCAGTGTCAC CAAGCAAATG AGTGCAGCCT TTGTGAAGCC
    CCATTTTTCC TCTTGGACAC TCAGTGCGTT TATAAGTGTG GGAAGGGATA TTATGCAGAC
    CACGCACAGC GAAAATGCAT AGCTTGTCCT CACGGGTGCT TGGAGTGTGA CAGTGACGGC
    CTTTGCCACC TCTGTGATGG CAACACCTTT CTGAAAAACA AGATTTGCGT TTCTGCCTGT
    GGACAGGGAT TCTATGGCAA TAAGTGGACC AGAGAGTGTG AAGTCAGCAG GCATCCTTCA
    AGCTTCCGTG TAAACAGTAC CCTCACAGTA GGGATTGGTG GGGTAAAGCC TCTAGACCTC
    TCCTTACTGG ATGTGCGTGA TCTGGATGGT GACACAGGAC AGCTCTTGTT CCATATTGAC
    AGCGCTCCCT CCAATGGCAG GCTAGTGATG GTGGTGAATG GGAAAGAAGC GCAGCTGAGC
    AAAGGTGACC ACTTCAGCTT CAAGGATGTG AAGGAGAATA GAGTCCGCTT TGTGCACAGC
    AGAGAGAAAT CCAGGAAAGG ACAGCTTTCC TTGAGAGTCA GCGATCAACA GTTCTCCCAT
    CCTGAGGTGA TCAACATTCA AGCATTTTCA ACACAGGCCC CCTATCTGCT GAGAAATGAG
    GCCCTCCTTG TCCGCAGAGG GGAAATTGGA ACTATAACAG ACCTGTTGCT TGATGTGAGA
    GATGGTGACA ATCCTCAGGA TGTCATGTTG ATGGTTTTAG AGCCTCCACG TCATGGTCAG
    CTGATAAAAC CTCCCAGGGA CTCAGCTTCT TCAGTCTACA TGTTCCACCT CGATGACCTT
    GCTGGTGGAC TGCTACGTTA TGCTCACGAT GGCTCTGACA CCCAGGATGA TGCAATTCTT
    CTGCAAGTGA ACGATGGATA CCACTTCCAG AACATCCTGT TCCACATTAA GATCGCTCCC
    AAGAGTTACA GAAGCCCTTG GCTGGTGACA AATTCCCTGG TCTGGGTGCC CCAGGGAGGC
    ATGCTGCAGA TCTCCAAGAC AATTCTGCAC GCTGAGGTAC CTGGAGCCCG TGACTCTGAG
    ATAACCTACA ACATCATCAA GGACCAGCCA AGACATGGTG AAGTGGTGCT TCTGGTCCCA
    ATGCCGGCAG ATGGCCCAGC AGAGTCATGG CAGCTTTTGC CTGATGGAAG AGCGGCCTCA
    CCTACAACCT CTTTTACCCA GCAGGACATC AATAATGGCA TCGTGTGGTA CAGGCACTCT
    GGAACTCAAG TAGAGAGTGA CTCCTTCCAG TTCCAGGTGT CCAGCTCTGC CAGTCCACAA
    GCCAGCTTGG AAAGCCACGT GTTCAACGTT GCTGTGCTCC CACAGACACC CAGAGCACCT
    CAGCTTTCCC TTGGCTCCTC TTTGCACATG GCTGTGCTGG AGGACCGCGT GACAGTGATT
    GAGCCCCATC ACCTCTCCTT TGTAGACCCA GAGATTCCCA GTGAGAAAAT CCTGTTCAAC
    GTGACTGTCC CTCTGCTTCC TGGCCAAGGT ATTGTGGAGC ACAGAGACAG GCCTCACTCG
    CCAGTCAGGT ATTTCACTCA AGCAGATATA AACCATGGCA AGATTGCTTA TCGTCCACCA
    ACTGCAGCTC CACACTTGAG AGAGATCATT GCATTCTCAT TTGCAGGTCT CCCAGAGTCA
    GTGAACTTCC GATTCACAGT GTCTGGTGGA GAACACACAA CCCCGGAGAT GGCGTTTGTC
    ATTCACTTGC TCTCTGCAGA GCAGCAGCCT CCAGTCTTCC AGATCACAGC TCCTTTCCTG
    GAGGTCAGCC AGGGGGGAAA GGCCACCATC GGTGTCCAGT TGGCTGTGAG TGACACAGAT
    ACAGCTCCTG AGGGCCTCTT CTTTGAGCTG GTGAAACCTC CCCATCATGG CATCGTGCTC
    AAGCATGATG CAGGTGTGCA GGAGCACATG AGAGCAGGTG ACACCTTCAC CTATGAGGAC
    GTCACTCGAA ATGCTCTGCA GTACGTACAT GATGGCTCAT CGGCAGCAGA AGACAGCATG
    GAAATCTCTG TCACCGATGG CGTCACCACA GTGACCACAG TGCTGAGAGT GGAGGTGTCC
    CTACTGGATA ACAACGGCCC ACAGCTGGCC CCAGGCTGCC TGTTGGCAAT CACTGTGGCT
    AGTAAAAGCT CTGCAACTCT CTCTCGACTG CACCTCGCTT ACATTGACAA CAATTCTCCT
    GACTCAGAGA TAAGAATCCA GTTAATATCT CTGCCCACGT ACGGCACCCT CTTACGGACG
    TCTGGCTCTC ATGCTGAAGA ACTTTCTAGG GTTTATAATT TCACCATGGA AGACATCAAC
    AACCAGAGGA TCAGTTACTC CACTGTGTTT GAGACAAGCA ATCAGCCAGT TACGGATGCC
    TTTCACTTCA CTGTTTTTGA TGCTGATAAT AACCGGCTTG ACAGACAGAT GTTTACCATC
    ACCATCACGT CTGCCCAGAC AGCACCATCA CTCATTGTGT TTGCTGACCA TATCGCTGTG
    GATGAGGGGG GCAGGGTCCC ACTTCTGGCT CATCACCACT TAACTGCTGA TTATGATACT
    GCTGCCAAGG AAGACCTGAG GGTCACGGTT ATCACTCTAC CCGTGTATGG CTACATTGAA
    AACACTAAGA CAGGTGAGAC TTTTGGACCA CAGAGTGCTG TGACCACAGA TGCATCCTTC
    TCCATTCAAG ATGTTCTAGA GAACAACATT TACTACTTTC AGAGCGTCCA TGAAAGCATC
    GAGCCAACAA GTGATGTTTT CAGCTTTTAC GTGAGCGATG GCTTCAGCCA GTCTGAGGTG
    CACAGCATCA ACATCACCAT TCAGCGACGG AATGATGAGC CCCCAAAGAT GGTGTTGGAG
    CCTGTCAGAG TGCATGAGAG CTCAGTGGTT GTTACCAGTG CCTCACTCAA CCTGCAGGAC
    CTGGACACCC AGAACAGTGA GCTTGTCATC ATGGTTACCA AAACTCCTGA GCACGGTCAC
    CTTCGCAGGA GGCAGACTGC TGTGGAGCCC CCAGAGCGTG GACACGTGCT GTCTCTGGGC
    TCATCCTTCA CCTACCAGGA CATTTTGGAT GAGCTGATTG TTTATTCTCA AAGCGGTGCA
    GCAGCACAGA CGGATGAGTT TGGCTTCTCC ATCACAGATG GTCTCTACAT GCAGACAGGG
    AAGCTGGAGT TCTCCATGGA GCTTCCAAAG AAGCAGCTGC CCACATTGAC TGCCAGCAGG
    GTCCTGCAGC TCCCCACTGG CTCTGCAGCA CACATCACAG ATCAACACTT GAAAGCAGCA
    GCTGTTTATC CAGATGACAC AATGATCAGA TTTGTCATGA AGAGGGATCC CAGCGTGGGC
    CATTTGCAGT TGACTGATAT TGATAATCCA GTCGAGATCT CTGTCAGAGG GCCTGTCAAA
    AGTTTTACCC AGGCTGATAT AAATAAAGGG CAAGTGGTTT ATAGTCACAA GAAGGGGGAT
    CCTGGTGGAA ATTTTGCCTT CACCTTTGAT GTGATCGATG CAGATGGCAG CAGACTGATG
    GACCAGTTTT TTCATATCAC TGTATTAGAA GACAGGTTCC CCCCCTCCAT CGTCGCTAAC
    ACAGGGCTGG TCTTAGATGA GAACTCTGCA AAGAAGATCA CAACACTGCA GCTCTCTGCC
    ACTGACCGGG ACAGTGAGCC GACCCAGCTC CAGTACAAGC TCACCAAGCA GCCACAGCTG
    GGGCACCTGG AGCATGTGGC CTCCCCAGGG ACAAAAATCA GTTCTTTCAG CCAGGCAGAC
    TTGGCCTCTC GCAGCATCCA ATACGTTCAC ACTAGCGACA CTGAAAAGCA TACGGATGCC
    TTCACCTTCT CTGTGTCTGA TGGAACAAAT GAGGTCAGCC AGACATTTGA CATCACCATA
    AACCCTGTGG ATGATTCCTT ACCTGTAGTG CAAAGCTTTG GGATGAGAGT TCAGGAAGGT
    GTGAGAAAAA CCATCACAGA GTTTGAGCTT AAAGCAACAG ATGCTGACAC AAAGGCTGAG
    GCAATTACAT TCACAGTCGT GCAGCCCCCT CGACATGGCC TGATTGAACA CACCAGCAAT
    GGGCAGCATT ACCACCAAGC CACCACCTTC ACCATGGATG ACATTTACCA GAACCGCATC
    AGCTACAGTC ACGATGGAAG CAACTCACTG AAGGATCGCT TTGCTTTCAC TGTGTCTGAT
    GGGACCAATC CCTTCTTCAT TATGGAGGAT GGGGGGAAAA AGGTTGTGAC AGCAGCACCA
    CAGCGCTTCA GAATGGACAT CCTCCCTGTG GATGATGGGA CACCCAGGGT GGTCACCAAC
    CAGGGTTTGC AATGGCTGGA GTATGTGGAT GGCAAGGCAA CCAATCTAAT CACCAAGAAG
    GAATTACTGA CAACAGATCC TGACACAGAT GACAAACAGC TGATCTATGA GATAACAGAT
    GGTCCCAGGA ATGGTTATGT GGAGAACAAG CTGAAACCAG GAACAACTGT GACCATGTTT
    TCTCAGGAGG ATGTGAATCA GGGCCTCATT CGGTATGTGA TGCATGAGGA GAAGGTTCAG
    GAGACTATGG ACAGTTTTCA GTTCCTCGTG AAGGACAGCA AACCCAATGT GGTCCGTGGC
    AACGTCTTCC ACATCCAGTG GTCTCTCCTC AGCTTTACAT ACAGCAGCTA CAACGTCAGT
    GAGAGCGCTG GCTCTGTGAG CGTCACCGTG CGGCGGATTG GTAACCTTAA CCAGTATGCC
    ATTGTCTTGT GCCGCACGGA GCAGGGAACA GCCACTTCCA GCTCTGGTTC ACAGTCTGAA
    GAGCAGGACT ATGTGGAGTA CGCAGGGCAG GTGCAGTTTG AGGAGCGGGA AGACACCAAG
    GCCTGCACCA TCGTCATTAA CGATGACGAC ATCTTTGAGA GCACCGAGAG CTTCACTGTG
    GAGCTCAGCA TGCCAGCCTA TGCTCTGCTG GGGAATGTCA CCCATGCCAC AGTCACCATC
    ACAGACGCTG AGGATGAGCC CATGCTGCAG TTCGACAGGA AGACGTACCA CGTCAGTGAG
    AGCGCTGGGT TTCTCTCTGC GCCTGTTGAA AGGAAAGGAG ATGCTAGCAG CACTGTGTCT
    GCTGTCTGCT ACACTGTCCC CAAATCAGCC AAGGGAAGCA GCCTTTATAT GCTGGAGTCT
    GGCTCCGACT TCAAGTCCCG AGGGATGACA GATGACAGCC GCATAGTTTT TGGACCAGGT
    GTTACCATGT CGACATGTGA TGTCATGCTT ATTGATGACA GTGAATATGA AGAAGAAGAG
    GAGTTTGAGA TAGTATTGGC TGATGCTTCG GAAAATGCAC GCATTGGCAA CCGGGCTTCA
    GCCAGCGTCT TCATCGGTGG TCCCAATGAT GCCTCGACAG TGTTCCTGGG AAATGCCACG
    TTCACCGTCA GTGAGGCTGC AGGAAACATT GAAATTCCAG TCCTCCGTCA CGGACCTGAT
    CTTTCAACCC CCACCTCAGT GTGGTGTGCT ACTCGGACAT CAGATCCTCC ATCTGCCAGC
    CCAGGAGTTG ATTATGTCCC CAGCTCCAGA AAAATAGAGT TCAGGCCAGG CAAGACAGAA
    GAGTTCTGCA CTCTGACAAT CCTGGATGAT GCTCAGTACC CAGTGATAGA AGGGCTGGAG
    ACATTTGTTG TTTATCTCAG CTCACCCCAT GGAGCTGAGC TGATAAAGCC CTTCCAAGCC
    ATTGTGGCCA TTAATGACAT CATCCAAGAT GTACCAACCA TGCAGTTCAC CAAGGATGTG
    TTCACAGTGA AGGAAAAGGA GGGTGCACTA CACATCCCCA TTTTCCGCAC TGGCGACCTG
    AGCTATGAGT CCTCCGTGAG GTGCTACACC CAGAGCCAGA CAGCAGAAGT CATGGAGGAC
    TTTGCAGAGA GGAGAAATGC AGACGAGTCC CGCATCACTT TCTTGAAAGG GGAGAAGGTG
    AAAAACTGCA GTGTTTACGT CAGTGATGAC TCTGCCTTTG AACCAGAGGA GCAGTTTCAT
    GTCCATCTTG GTAGTCCGCT TGGAAACCAT TGGAGTGGAG CTAGGATTGG GAAGATAGAC
    ATGGCAACTG TGACCATCAC TAATGATGAA GACGCACCCA CCATTGAGTT TGAAGAAGCT
    GCGTACCAGG TACGGGAGCC ACCTGGACCA GAGGGTGTCG CTGCTTTAAA TATCAAAGTG
    GTCCGGAGAG GGGATCAGAA CAGGACCTCA AAAGTGCGCT GTAGCACTCG TGATGGCTCA
    GCTCAGGCCG GAGTGGATTA CTACCCCAAG AGTCGAGTGC TCAAATTCAG CCCAGGTGTG
    GACCACATCA TGTTTAAGGT GGAGATTATG TCCAACGAAG ATCGGGAGTG GCACGAGTCC
    TTTTCTCTGG TGCTGGGTCC AGATGACCCA GTGGAAGCTG TTCTTGGAGA CACCAGCACT
    GCAACAGTGA CTATTTTGGA TCAGGAGGCA GCAGGCAGCC TGATTCTGCC AGCACCTCCT
    GTGGTCGTCT CCTTGGCTGA TTATGACCGT GTGGAAGAAG CGACCAAGCA CGGTGCTAAG
    AAATCCCCTT CTCCAGGCTA TCCTCTCATC TGTGTCACTC CCTGTGATCC TCACTTCCCC
    AAGTTCATGG TCATGAAGGA GCGCTGCAGC GAGGCCGGGA TCAACCAGTC TTCCATACAG
    TTCAGCTGGG AAGTAGCAAC CCCCACAGAT GGGAATGGGG CCAGGTCGCC TTTTGAAACC
    ATCACTGACA ACACACCATT CACCAGCGTC AACCATAAGG TGCTGGACAG CATTTATTTC
    AGCCGGCGGT TCCACGTGCG CTGTGTGGCC AAGGCTGTGG ACAAGGCTGG CCATGTGGGC
    ACCCCCTTGA GGAGCAACGC AGTTACTATA GGGACAGACA GTGCCATTTG TCACACTCCT
    GTGGTCGCGG GGACAGCTCG AGGCTTCCAG GCACAGTCGT TTGTTGCCAC TCTGAAGTAT
    CTGGACGTCA AACACAAGGA GCATCCCAAC AGGATCCACA TCTCAGTGCA GATCCCCCAC
    CAGGATGGAA TGCTGCCACT CATCTCCACC CTGCCCCTGC ACAATCTGCA TTTCCTGCTC
    TCTGAGTCTA TCTACAGACA CCAGCATGTC TGCTCAAACC TGGTCACCAT CAGAGACCTT
    AAAGGCATCT CAGAGGCAGG ATTCCTTGAT GAAGTGACAT ATGATAGCAT TGTTCTCGGT
    CCTGGCTATG ACCGCCCTTA CCAGTTCGAC CCCTCCGTGA GAGAGCCAAA GACAATCCAG
    CTGTACAAGC ACCTCAATCT GAAGAGCTGC ATATGGACCT TTGATGCCTA TTACGACATG
    ACTGAATTAA TTGATGTCTG TGGAGGCTCT GTAACAGCTG ACTTCCAGGT ACGGGACTCT
    GCTCAGTCCT TCCTAACAGT CCACGTCCCC CTCTATGTGT CCTATATTTA TGTGACAGCG
    CCGAGAGGCT GGGCTTCTCT TGAGCATCAC ACAGAGATGG AGTTCTCCTT TTTCTACGAC
    ACTGTTCTCT GGAGGACAGG GATTCAGACG GACAGTGTCC TCTCAGCCCG GCTGCAGATA
    ATCAGAATCT ACATCCGTGA GGATGGCCGA CTGGTTATTG AGTTCAAGAC CCAGGCCAAG
    TTCAGAGGGC TCTTTGTAAT GGAGCACCAC AGCTTGCCAG ATGTGAAGTC GTCTTTGATG
    ACTCCAGACC ATCTGGGAGG GATCGAATTT GACCTGCAAC TGCTGTGGAG TGCTCAGACT
    TTTGACTCTC CCTACCAGCT CTGGAGGGCA ACCAGCTCTT ATAACAGGAA GGACTACTCT
    GGAGAGTACA CAATCCTCTT AATTCCTTGT ACTGTGCAGC CTACACAGCC ATGGATAGAG
    CCTGGAGAGA AGCCCCTGCC CTGTACAGCT CATGCTCCCG AGCGATTTTT GATCCCAATC
    GCCTTCCAGC AGACAAACCG CCCAGTTCCT GTTGTCTACT CCCTAAACAC GGAGTTTCAG
    CTGTGTAACA ATGAGAAGGT GTTTCTGATG GATCCCACCA AGTCCGAAAT GTCTCTGGCA
    GAAATGGATT ACAAAGGGGC TTTTTCAAAA GGGCAAATCC TGTATGGCCG TGTGCTCTGG
    AATCCTGAAC AAAACCTAAA CGCTGCTTAC AAACTGCAGC TGGAGAAAGT CTACCTGTGC
    ACTGGCAAGG ATGGCTACGT GCCCTTCTTT GACCCAACAG GAACTGTCTA TAACGAAGGG
    CCTCAGTATG GCTGTATTCA ACCCAACAAG CACCTGAAAC ACAGATTTCT CCTCTTGGAC
    CGAAACCAAC CAGAAGTCAC AGATAAGTAT TTCCACGATG TGCCTTTTGA TGCCCACTTT
    GCCTCTGAAC TGTCTGAGTT TCACTCAGTA AGCAGCATGC CTGGTGTGGA TGGATTTACT
    CTCAAGGTGG ATGCTCTCTA CAAGGTGGAA GCTGGACATC AGTGGTACCT TCAAGTTATC
    TACATAATAG GCCCAGAGAG CATTGCGGGG CCTCGTGTTC AACGATCTCT TACTCACCAC
    TTAAAGCGTG GCCGCAGGGA CCTGGTTGAC AGTGATGGGA GGCTGATGCT GGATGACTCC
    TTGATCTACG ACAACGAGGG CGATCAGGTC AAGAACGGCA CCAACATGAA GTCGCTGATC
    TTGGAGATGG AGGAAGCTGT GGTCGCAGCC TCCTTATCAC AGACGGGTGC TTCCCTCGGG
    AGTGCATTTG CTGCTGTCAT GCTGCTGTTG CTGGTGCTTC TGGGAGTCTG CCTTGTCACC
    AGGAAGTGCA GGACGCTGCG GAGGAAGCGA GAGGCTGTCC GAGCTGCCCC AGAGGAACAC
    CCTCTGAACA CTAAGGTGGA GCTGCCCAAG GGCACAGAGA AGGCAGTGAA CAGCCAGTAC
    TGCACCGTGA GGAACATCAA TATACTGAGG GAAACAGGGG GCATCTCCAG TGTCAAAAGC
    AGAAAGGTGA AGCAGGTGAA CTTAGAAGTT AAAGTCCACA ATAATTTGCA CGACGGAACA
    GAAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025006060.1
    CDS459..12287
    Translation

    Target ORF information:

    RefSeq Version XM_025150292.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025150292.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    ATGGGCGCCT GGCTGCCGCT GTGCCTGCTC AGCGCCGCGC TGGCCGCCGC CGCCGCCGCC 
    GCCTCAGGTG CCTGTGTCTA TCAAGGCTTG TTGTTTGAGA ATAACACAAT TTGGAAGCCA
    GATTCTTGCC AAGACTGCAG TTGCCAAAGC AATGTTGTCA CCTGTGAACC TGCTGTCTGC
    GAACATCCTC AGTGTGACTT TGAGAAGGGA GAAGTGCTCC AAATAGCCCC AAACAAATGT
    TGCCCTAAAT GTGCCTCACA AACTGAAGGA TATTGCCAGC ATGAAGGACA GATCCACAGT
    CATGGCACGC AGTGGGCCAG CTCTGGATGT GTTCTGTGCT CCTGTGCCCA TGGGAAGGTG
    AGCTGCACGC CCATAGCCTG CCCAGTCCTC ACCTGTGAAA GGGATGAGCT GCCATACACT
    GCCCAGGGAG CCTGTTGTCC CACATGCGTG GGCCTTGGTG AGCCCTGCTA CTTTGATGGC
    CGTTCGTTCC GGGATGGTGA GGACTGGCGC CTTGGTCGGT GCTCCAAATG TGTCTGCAGG
    GATGGCGTGA CTCAGTGCTT CACAGCTTCC TGTGAGGCTG TTCTGTGCAA ACAGGATGAA
    GTTTTGGCTA TGTCTCCTGG AAAATGCTGT CCAGAATGTG TTCCAAAGTC CTGCTCGGTG
    TCAGGAAAGG TGTACCAGCA CGGAGAACGG TGGAAAAAAA ATGCCTGCAG TGCGTGTGCG
    TGTCATAGAG GGGCAGTCAG ATGTCTGAGA GAAACCTGTG ACTCTGTTAT TTGTGAGAAG
    GGGGATAACA AAGTGCAGCG TTCCGGGAAG TGCTGTGAGG AGTGTGTGTC TTCCAAGGAA
    AACTGCTTGT ATGATGGCAC TATCAGGTAC CACGGTGAGA TGTGGAATAT TACTCGCTGT
    GACTTCTGCA TGTGTGATGA AGGTCAAGTG ACTTGTCACA AAGCAGAGTG CGCCAAAGTA
    GAATGTGCCA AGGGTGAAGA ATTAATTCAC TTAGATGGGA AGTGCTGTCC TGAATGTATT
    TCAAGTAATC GTCATTGTTT TTACAAAGGA CATACCAAAG CTAGCGGAGA GACGTGGAAG
    GAAGGGCTGT GCAGAGAGTG TGAATGCCGC GACTCAGAGG TGGTCTGCTT TCAGCTTTCC
    TGTCCTCCCT GCCCACCGGG CAGCATGGCT GTCAGGGAGA AGGGTGATTG CTGTCCACGC
    TGCCAGCCAG GAAAGTGCCA CCCAGACTGT TTGACCTGCT CGCAGTCCTT TGATCACTGT
    ATTGCCTGCC GTGATGTGAA GAAGCAGCTG CAGAATGGGC GCTGTGTGGA GAGCTGTGGG
    TCAGGCTTCT ATCAGGATGG GGGGACTTGC TTAGCCTGCA ATGAAACATG CTCAGCCTGT
    ACGAATGGTT TTGAGTGTTC CTCATGCCAG ACGTCCCTGC TGATGAAGAA TGGGCAGTGT
    GTGACTTCAT GTGGGAAGGG ATTCTTTCAG GATCAACTCA TATGTGCAGT TTGTCATGAG
    TCCTGTTCCT CGTGTTGGGG ACCTGCTGAC AACAGTTGTC TCTCCTGCAA AGACACGTCA
    CATGTGCTAA AAGCAGGGCT CTGCGTGGCC AGCTGTGGAA AGGGATTTTA TGAGAAGGAT
    GGAATCTGCA GTGTTTGTGA CCCGTCCTGT GAAACGTGCA ATTCAGCCCA GCCCAGTTGT
    TTAACCTGTG CTTCAGATAA GGTGTTACAC AACGGGAACT GCATTTCAGA GTGCCCAGGC
    GGATATTTCC CAGGCAGCAA TGGAAGATGC AGGGCTTGTC ATGATTCGTG CTCCACCTGT
    GAGGGACCTC TGGCCACTCA CTGTACCTCC TGTTCCCTTC CTCTGGCTTT GCACCAGGGC
    CAGTGCCTGC AGGGCTGTGG AGAGGGCTTT TATCAGGATC ACAGCATTTG CAGGGGTTGC
    CACCCATCCT GCCGTAACTG CATTGGCCCA GGGGCCTCTC ATTGCCTGCA GTGCTGGGCA
    CCAGAGGATG TGCTGCAGCC CCATCAACCT GTGGAAGGAG CTGTGCATGG CTTTTGCCTC
    TCACACTGCC AGGCCCAGTT CTACCTGGAT GTCACAGGAG TCTGCAAACA GTGCCACTCC
    TCCTGTGCAG GCTGCATAGG GAACACTTCC CAGGACTGTA CGGCCTGTCC GTCTTCGCAC
    ATGCTGCTCG AGGGCAGATG CCTCTCCGAG TGTCCAGAGG GGTTGTTCAG CCAGCAGGAT
    CACTGCTCCT CCTGCCATCC GTCATGCAAG ACATGCCATG GCCCTTCTGA TTTGGAGTGC
    TTAACCTGCC ATCCCCATGC AACTCTGGCT GCTGGAAGGT GCAGGACTAG CTGCAAGGAA
    GAGCAGTATC TCAACTTGGT TGGATATTGC GTGGACTGCC ATCCTTTGTG CCATCAGTGT
    GTGGCCAACC TGTGGGACAC TGGCAGCCTC TGTCTCAAGT GCCAGAATGC CCGTCACTTG
    CTCCTTGGGG ATCACTGCGT TCCTGACTGT CCAGCAGGAT ACTACACAGA GAGCGGCGCC
    TGCAAACGAT GTCACCCCTC ATGTAAAACC TGCAGAGGTA GGGGTCCTCT TTCCTGCTCC
    TCCTGCAACA CCAGCCTGGT TCTGTCCCAC ACCAGCATGT GCGTTCCAGT CTGTTCTCCG
    GGATACTACA AGGATCAGAG CCAGACCTGC AAACCTTGCA ACAGCCAGTG CCTGAACTGT
    GAATCAGCTA CAGGCTGTAT GTCTTGCAGA GATCCAGCTA AGGTCCTGTT ATTTGGAGAA
    TGCCAGTATG AGAGCTGTGC TCAGCAGTAT TATCTGGATT TTTCAACCAA GACTTGCAAA
    GGGTGTGATC AACATTGCCT TCAGTGTCAC CAAGCAAATG AGTGCAGCCT TTGTGAAGCC
    CCATTTTTCC TCTTGGACAC TCAGTGCGTT TATAAGTGTG GGAAGGGATA TTATGCAGAC
    CACGCACAGC GAAAATGCAT AGCTTGTCCT CACGGGTGCT TGGAGTGTGA CAGTGACGGC
    CTTTGCCACC TCTGTGATGG CAACACCTTT CTGAAAAACA AGATTTGCGT TTCTGCCTGT
    GGACAGGGAT TCTATGGCAA TAAGTGGACC AGAGAGTGTG AAGTCAGCAG GCATCCTTCA
    AGCTTCCGTG TAAACAGTAC CCTCACAGTA GGGATTGGTG GGGTAAAGCC TCTAGACCTC
    TCCTTACTGG ATGTGCGTGA TCTGGATGGT GACACAGGAC AGCTCTTGTT CCATATTGAC
    AGCGCTCCCT CCAATGGCAG GCTAGTGATG GTGGTGAATG GGAAAGAAGC GCAGCTGAGC
    AAAGGTGACC ACTTCAGCTT CAAGGATGTG AAGGAGAATA GAGTCCGCTT TGTGCACAGC
    AGAGAGAAAT CCAGGAAAGG ACAGCTTTCC TTGAGAGTCA GCGATCAACA GTTCTCCCAT
    CCTGAGGTGA TCAACATTCA AGCATTTTCA ACACAGGCCC CCTATCTGCT GAGAAATGAG
    GCCCTCCTTG TCCGCAGAGG GGAAATTGGA ACTATAACAG ACCTGTTGCT TGATGTGAGA
    GATGGTGACA ATCCTCAGGA TGTCATGTTG ATGGTTTTAG AGCCTCCACG TCATGGTCAG
    CTGATAAAAC CTCCCAGGGA CTCAGCTTCT TCAGTCTACA TGTTCCACCT CGATGACCTT
    GCTGGTGGAC TGCTACGTTA TGCTCACGAT GGCTCTGACA CCCAGGATGA TGCAATTCTT
    CTGCAAGTGA ACGATGGATA CCACTTCCAG AACATCCTGT TCCACATTAA GATCGCTCCC
    AAGAGTTACA GAAGCCCTTG GCTGGTGACA AATTCCCTGG TCTGGGTGCC CCAGGGAGGC
    ATGCTGCAGA TCTCCAAGAC AATTCTGCAC GCTGAGGTAC CTGGAGCCCG TGACTCTGAG
    ATAACCTACA ACATCATCAA GGACCAGCCA AGACATGGTG AAGTGGTGCT TCTGGTCCCA
    ATGCCGGCAG ATGGCCCAGC AGAGTCATGG CAGCTTTTGC CTGATGGAAG AGCGGCCTCA
    CCTACAACCT CTTTTACCCA GCAGGACATC AATAATGGCA TCGTGTGGTA CAGGCACTCT
    GGAACTCAAG TAGAGAGTGA CTCCTTCCAG TTCCAGGTGT CCAGCTCTGC CAGTCCACAA
    GCCAGCTTGG AAAGCCACGT GTTCAACGTT GCTGTGCTCC CACAGACACC CAGAGCACCT
    CAGCTTTCCC TTGGCTCCTC TTTGCACATG GCTGTGCTGG AGGACCGCGT GACAGTGATT
    GAGCCCCATC ACCTCTCCTT TGTAGACCCA GAGATTCCCA GTGAGAAAAT CCTGTTCAAC
    GTGACTGTCC CTCTGCTTCC TGGCCAAGGT ATTGTGGAGC ACAGAGACAG GCCTCACTCG
    CCAGTCAGGT ATTTCACTCA AGCAGATATA AACCATGGCA AGATTGCTTA TCGTCCACCA
    ACTGCAGCTC CACACTTGAG AGAGATCATT GCATTCTCAT TTGCAGGTCT CCCAGAGTCA
    GTGAACTTCC GATTCACAGT GTCTGGTGGA GAACACACAA CCCCGGAGAT GGCGTTTGTC
    ATTCACTTGC TCTCTGCAGA GCAGCAGCCT CCAGTCTTCC AGATCACAGC TCCTTTCCTG
    GAGGTCAGCC AGGGGGGAAA GGCCACCATC GGTGTCCAGT TGGCTGTGAG TGACACAGAT
    ACAGCTCCTG AGGGCCTCTT CTTTGAGCTG GTGAAACCTC CCCATCATGG CATCGTGCTC
    AAGCATGATG CAGGTGTGCA GGAGCACATG AGAGCAGGTG ACACCTTCAC CTATGAGGAC
    GTCACTCGAA ATGCTCTGCA GTACGTACAT GATGGCTCAT CGGCAGCAGA AGACAGCATG
    GAAATCTCTG TCACCGATGG CGTCACCACA GTGACCACAG TGCTGAGAGT GGAGGTGTCC
    CTACTGGATA ACAACGGCCC ACAGCTGGCC CCAGGCTGCC TGTTGGCAAT CACTGTGGCT
    AGTAAAAGCT CTGCAACTCT CTCTCGACTG CACCTCGCTT ACATTGACAA CAATTCTCCT
    GACTCAGAGA TAAGAATCCA GTTAATATCT CTGCCCACGT ACGGCACCCT CTTACGGACG
    TCTGGCTCTC ATGCTGAAGA ACTTTCTAGG GTTTATAATT TCACCATGGA AGACATCAAC
    AACCAGAGGA TCAGTTACTC CACTGTGTTT GAGACAAGCA ATCAGCCAGT TACGGATGCC
    TTTCACTTCA CTGTTTTTGA TGCTGATAAT AACCGGCTTG ACAGACAGAT GTTTACCATC
    ACCATCACGT CTGCCCAGAC AGCACCATCA CTCATTGTGT TTGCTGACCA TATCGCTGTG
    GATGAGGGGG GCAGGGTCCC ACTTCTGGCT CATCACCACT TAACTGCTGA TTATGATACT
    GCTGCCAAGG AAGACCTGAG GGTCACGGTT ATCACTCTAC CCGTGTATGG CTACATTGAA
    AACACTAAGA CAGGTGAGAC TTTTGGACCA CAGAGTGCTG TGACCACAGA TGCATCCTTC
    TCCATTCAAG ATGTTCTAGA GAACAACATT TACTACTTTC AGAGCGTCCA TGAAAGCATC
    GAGCCAACAA GTGATGTTTT CAGCTTTTAC GTGAGCGATG GCTTCAGCCA GTCTGAGGTG
    CACAGCATCA ACATCACCAT TCAGCGACGG AATGATGAGC CCCCAAAGAT GGTGTTGGAG
    CCTGTCAGAG TGCATGAGAG CTCAGTGGTT GTTACCAGTG CCTCACTCAA CCTGCAGGAC
    CTGGACACCC AGAACAGTGA GCTTGTCATC ATGGTTACCA AAACTCCTGA GCACGGTCAC
    CTTCGCAGGA GGCAGACTGC TGTGGAGCCC CCAGAGCGTG GACACGTGCT GTCTCTGGGC
    TCATCCTTCA CCTACCAGGA CATTTTGGAT GAGCTGATTG TTTATTCTCA AAGCGGTGCA
    GCAGCACAGA CGGATGAGTT TGGCTTCTCC ATCACAGATG GTCTCTACAT GCAGACAGGG
    AAGCTGGAGT TCTCCATGGA GCTTCCAAAG AAGCAGCTGC CCACATTGAC TGCCAGCAGG
    GTCCTGCAGC TCCCCACTGG CTCTGCAGCA CACATCACAG ATCAACACTT GAAAGCAGCA
    GCTGTTTATC CAGATGACAC AATGATCAGA TTTGTCATGA AGAGGGATCC CAGCGTGGGC
    CATTTGCAGT TGACTGATAT TGATAATCCA GTCGAGATCT CTGTCAGAGG GCCTGTCAAA
    AGTTTTACCC AGGCTGATAT AAATAAAGGG CAAGTGGTTT ATAGTCACAA GAAGGGGGAT
    CCTGGTGGAA ATTTTGCCTT CACCTTTGAT GTGATCGATG CAGATGGCAG CAGACTGATG
    GACCAGTTTT TTCATATCAC TGTATTAGAA GACAGGTTCC CCCCCTCCAT CGTCGCTAAC
    ACAGGGCTGG TCTTAGATGA GAACTCTGCA AAGAAGATCA CAACACTGCA GCTCTCTGCC
    ACTGACCGGG ACAGTGAGCC GACCCAGCTC CAGTACAAGC TCACCAAGCA GCCACAGCTG
    GGGCACCTGG AGCATGTGGC CTCCCCAGGG ACAAAAATCA GTTCTTTCAG CCAGGCAGAC
    TTGGCCTCTC GCAGCATCCA ATACGTTCAC ACTAGCGACA CTGAAAAGCA TACGGATGCC
    TTCACCTTCT CTGTGTCTGA TGGAACAAAT GAGGTCAGCC AGACATTTGA CATCACCATA
    AACCCTGTGG ATGATTCCTT ACCTGTAGTG CAAAGCTTTG GGATGAGAGT TCAGGAAGGT
    GTGAGAAAAA CCATCACAGA GTTTGAGCTT AAAGCAACAG ATGCTGACAC AAAGGCTGAG
    GCAATTACAT TCACAGTCGT GCAGCCCCCT CGACATGGCC TGATTGAACA CACCAGCAAT
    GGGCAGCATT ACCACCAAGC CACCACCTTC ACCATGGATG ACATTTACCA GAACCGCATC
    AGCTACAGTC ACGATGGAAG CAACTCACTG AAGGATCGCT TTGCTTTCAC TGTGTCTGAT
    GGGACCAATC CCTTCTTCAT TATGGAGGAT GGGGGGAAAA AGGTTGTGAC AGCAGCACCA
    CAGCGCTTCA GAATGGACAT CCTCCCTGTG GATGATGGGA CACCCAGGGT GGTCACCAAC
    CAGGGTTTGC AATGGCTGGA GTATGTGGAT GGCAAGGCAA CCAATCTAAT CACCAAGAAG
    GAATTACTGA CAACAGATCC TGACACAGAT GACAAACAGC TGATCTATGA GATAACAGAT
    GGTCCCAGGA ATGGTTATGT GGAGAACAAG CTGAAACCAG GAACAACTGT GACCATGTTT
    TCTCAGGAGG ATGTGAATCA GGGCCTCATT CGGTATGTGA TGCATGAGGA GAAGGTTCAG
    GAGACTATGG ACAGTTTTCA GTTCCTCGTG AAGGACAGCA AACCCAATGT GGTCCGTGGC
    AACGTCTTCC ACATCCAGTG GTCTCTCCTC AGCTTTACAT ACAGCAGCTA CAACGTCAGT
    GAGAGCGCTG GCTCTGTGAG CGTCACCGTG CGGCGGATTG GTAACCTTAA CCAGTATGCC
    ATTGTCTTGT GCCGCACGGA GCAGGGAACA GCCACTTCCA GCTCTGGTTC ACAGTCTGAA
    GAGCAGGACT ATGTGGAGTA CGCAGGGCAG GTGCAGTTTG AGGAGCGGGA AGACACCAAG
    GCCTGCACCA TCGTCATTAA CGATGACGAC ATCTTTGAGA GCACCGAGAG CTTCACTGTG
    GAGCTCAGCA TGCCAGCCTA TGCTCTGCTG GGGAATGTCA CCCATGCCAC AGTCACCATC
    ACAGACGCTG AGGATGAGCC CATGCTGCAG TTCGACAGGA AGACGTACCA CGTCAGTGAG
    AGCGCTGGGT TTCTCTCTGC GCCTGTTGAA AGGAAAGGAG ATGCTAGCAG CACTGTGTCT
    GCTGTCTGCT ACACTGTCCC CAAATCAGCC AAGGGAAGCA GCCTTTATAT GCTGGAGTCT
    GGCTCCGACT TCAAGTCCCG AGGGATGACA GATGACAGCC GCATAGTTTT TGGACCAGGT
    GTTACCATGT CGACATGTGA TGTCATGCTT ATTGATGACA GTGAATATGA AGAAGAAGAG
    GAGTTTGAGA TAGTATTGGC TGATGCTTCG GAAAATGCAC GCATTGGCAA CCGGGCTTCA
    GCCAGCGTCT TCATCGGTGG TCCCAATGAT GCCTCGACAG TGTTCCTGGG AAATGCCACG
    TTCACCGTCA GTGAGGCTGC AGGAAACATT GAAATTCCAG TCCTCCGTCA CGGACCTGAT
    CTTTCAACCC CCACCTCAGT GTGGTGTGCT ACTCGGACAT CAGATCCTCC ATCTGCCAGC
    CCAGGAGTTG ATTATGTCCC CAGCTCCAGA AAAATAGAGT TCAGGCCAGG CAAGACAGAA
    GAGTTCTGCA CTCTGACAAT CCTGGATGAT GCTCAGTACC CAGTGATAGA AGGGCTGGAG
    ACATTTGTTG TTTATCTCAG CTCACCCCAT GGAGCTGAGC TGATAAAGCC CTTCCAAGCC
    ATTGTGGCCA TTAATGACAT CATCCAAGAT GTACCAACCA TGCAGTTCAC CAAGGATGTG
    TTCACAGTGA AGGAAAAGGA GGGTGCACTA CACATCCCCA TTTTCCGCAC TGGCGACCTG
    AGCTATGAGT CCTCCGTGAG GTGCTACACC CAGAGCCAGA CAGCAGAAGT CATGGAGGAC
    TTTGCAGAGA GGAGAAATGC AGACGAGTCC CGCATCACTT TCTTGAAAGG GGAGAAGGTG
    AAAAACTGCA GTGTTTACGT CAGTGATGAC TCTGCCTTTG AACCAGAGGA GCAGTTTCAT
    GTCCATCTTG GTAGTCCGCT TGGAAACCAT TGGAGTGGAG CTAGGATTGG GAAGATAGAC
    ATGGCAACTG TGACCATCAC TAATGATGAA GACGCACCCA CCATTGAGTT TGAAGAAGCT
    GCGTACCAGG TACGGGAGCC ACCTGGACCA GAGGGTGTCG CTGCTTTAAA TATCAAAGTG
    GTCCGGAGAG GGGATCAGAA CAGGACCTCA AAAGTGCGCT GTAGCACTCG TGATGGCTCA
    GCTCAGGCCG GAGTGGATTA CTACCCCAAG AGTCGAGTGC TCAAATTCAG CCCAGGTGTG
    GACCACATCA TGTTTAAGGT GGAGATTATG TCCAACGAAG ATCGGGAGTG GCACGAGTCC
    TTTTCTCTGG TGCTGGGTCC AGATGACCCA GTGGAAGCTG TTCTTGGAGA CACCAGCACT
    GCAACAGTGA CTATTTTGGA TCAGGAGGCA GCAGGCAGCC TGATTCTGCC AGCACCTCCT
    GTGGTCGTCT CCTTGGCTGA TTATGACCGT GTGGAAGAAG CGACCAAGCA CGGTGCTAAG
    AAATCCCCTT CTCCAGGCTA TCCTCTCATC TGTGTCACTC CCTGTGATCC TCACTTCCCC
    AAGTTCATGG TCATGAAGGA GCGCTGCAGC GAGGCCGGGA TCAACCAGTC TTCCATACAG
    TTCAGCTGGG AAGTAGCAAC CCCCACAGAT GGGAATGGGG CCAGGTCGCC TTTTGAAACC
    ATCACTGACA ACACACCATT CACCAGCGTC AACCATAAGG TGCTGGACAG CATTTATTTC
    AGCCGGCGGT TCCACGTGCG CTGTGTGGCC AAGGCTGTGG ACAAGGCTGG CCATGTGGGC
    ACCCCCTTGA GGAGCAACGC AGTTACTATA GGGACAGACA GTGCCATTTG TCACACTCCT
    GTGGTCGCGG GGACAGCTCG AGGCTTCCAG GCACAGTCGT TTGTTGCCAC TCTGAAGTAT
    CTGGACGTCA AACACAAGGA GCATCCCAAC AGGATCCACA TCTCAGTGCA GATCCCCCAC
    CAGGATGGAA TGCTGCCACT CATCTCCACC CTGCCCCTGC ACAATCTGCA TTTCCTGCTC
    TCTGAGTCTA TCTACAGACA CCAGCATGTC TGCTCAAACC TGGTCACCAT CAGAGACCTT
    AAAGGCATCT CAGAGGCAGG ATTCCTTGAT GAAGTGACAT ATGATAGCAT TGTTCTCGGT
    CCTGGCTATG ACCGCCCTTA CCAGTTCGAC CCCTCCGTGA GAGAGCCAAA GACAATCCAG
    CTGTACAAGC ACCTCAATCT GAAGAGCTGC ATATGGACCT TTGATGCCTA TTACGACATG
    ACTGAATTAA TTGATGTCTG TGGAGGCTCT GTAACAGCTG ACTTCCAGGT ACGGGACTCT
    GCTCAGTCCT TCCTAACAGT CCACGTCCCC CTCTATGTGT CCTATATTTA TGTGACAGCG
    CCGAGAGGCT GGGCTTCTCT TGAGCATCAC ACAGAGATGG AGTTCTCCTT TTTCTACGAC
    ACTGTTCTCT GGAGGACAGG GATTCAGACG GACAGTGTCC TCTCAGCCCG GCTGCAGATA
    ATCAGAATCT ACATCCGTGA GGATGGCCGA CTGGTTATTG AGTTCAAGAC CCAGGCCAAG
    TTCAGAGGGC TCTTTGTAAT GGAGCACCAC AGCTTGCCAG ATGTGAAGTC GTCTTTGATG
    ACTCCAGACC ATCTGGGAGG GATCGAATTT GACCTGCAAC TGCTGTGGAG TGCTCAGACT
    TTTGACTCTC CCTACCAGCT CTGGAGGGCA ACCAGCTCTT ATAACAGGAA GGACTACTCT
    GGAGAGTACA CAATCCTCTT AATTCCTTGT ACTGTGCAGC CTACACAGCC ATGGATAGAG
    CCTGGAGAGA AGCCCCTGCC CTGTACAGCT CATGCTCCCG AGCGATTTTT GATCCCAATC
    GCCTTCCAGC AGACAAACCG CCCAGTTCCT GTTGTCTACT CCCTAAACAC GGAGTTTCAG
    CTGTGTAACA ATGAGAAGGT GTTTCTGATG GATCCCACCA AGTCCGAAAT GTCTCTGGCA
    GAAATGGATT ACAAAGGGGC TTTTTCAAAA GGGCAAATCC TGTATGGCCG TGTGCTCTGG
    AATCCTGAAC AAAACCTAAA CGCTGCTTAC AAACTGCAGC TGGAGAAAGT CTACCTGTGC
    ACTGGCAAGG ATGGCTACGT GCCCTTCTTT GACCCAACAG GAACTGTCTA TAACGAAGGG
    CCTCAGTATG GCTGTATTCA ACCCAACAAG CACCTGAAAC ACAGATTTCT CCTCTTGGAC
    CGAAACCAAC CAGAAGTCAC AGATAAGTAT TTCCACGATG TGCCTTTTGA TGCCCACTTT
    GCCTCTGAAC TGTCTGAGTT TCACTCAGTA AGCAGCATGC CTGGTGTGGA TGGATTTACT
    CTCAAGGTGG ATGCTCTCTA CAAGGTGGAA GCTGGACATC AGTGGTACCT TCAAGTTATC
    TACATAATAG GCCCAGAGAG CATTGCGGGG CCTCGTGTTC AACGATCTCT TACTCACCAC
    TTAAAGCGTG GCCGCAGGGA CCTGGTTGAC AGTGATGGGA GGCTGATGCT GGATGACTCC
    TTGATCTACG ACAACGAGGG CGATCAGGTC AAGAACGGCA CCAACATGAA GTCGCTGATC
    TTGGAGATGG AGGAAGCTGT GGTCGCAGCC TCCTTATCAC AGACGGGTGC TTCCCTCGGG
    AGTGCATTTG CTGCTGTCAT GCTGCTGTTG CTGGTGCTTC TGGGAGTCTG CCTTGTCACC
    AGGAAGTGCA GGACGCTGCG GAGGAAGCGA GAGGCTGTCC GAGCTGCCCC AGAGGAACAC
    CCTCTGAACA CTAAGGTGGA GCTGCCCAAG GGCACAGAGA AGGCAGTGAA CAGCCAGTAC
    TGCACCGTGA GGAACATCAA TATACTGAGG GAAACAGGGG GCATCTCCAG TGTCAAAAGC
    AGAAAGGTGA AGCAGGTGAA CTTAGAAGTT AAAGTCCACA ATAATTTGCA CGACGGAACA
    GAAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35354
    Clone ID Related Accession (Same CDS sequence) XM_015276618.1 , XM_015276618.2
    Accession Version XM_015276618.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8583bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product extracellular matrix protein FRAS1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGGGCGCCT GGCTGCCGCT GTGCCTGCTC AGCGCCGCGC TGGCCGCCGC CGCCGCCGCC 
    GCCTCAGGTG CCTGTGTCTA TCAAGGCTTG TTGTTTGAGA ATAACACAAT TTGGAAGCCA
    GATTCTTGCC AAGACTGCAG TTGCCAAAGC AATGTTGTCA CCTGTGAACC TGCTGTCTGC
    GAACATCCTC AGTGTGACTT TGAGAAGGGA GAAGTGCTCC AAATAGCCCC AAACAAATGT
    TGCCCTAAAT GTGCCTCACA AACTGAAGGA TATTGCCAGC ATGAAGGACA GATCCACAGT
    CATGGCACGC AGTGGGCCAG CTCTGGATGT GTTCTGTGCT CCTGTGCCCA TGGGAAGGTG
    AGCTGCACGC CCATAGCCTG CCCAGTCCTC ACCTGTGAAA GGGATGAGCT GCCATACACT
    GCCCAGGGAG CCTGTTGTCC CACATGCGTG GGCCTTGGTG AGCCCTGCTA CTTTGATGGC
    CGTTCGTTCC GGGATGGTGA GGACTGGCGC CTTGGTCGGT GCTCCAAATG TGTCTGCAGG
    GATGGCGTGA CTCAGTGCTT CACAGCTTCC TGTGAGGCTG TTCTGTGCAA ACAGGATGAA
    GTTTTGGCTA TGTCTCCTGG AAAATGCTGT CCAGAATGTG TTCCAAAGTC CTGCTCGGTG
    TCAGGAAAGG TGTACCAGCA CGGAGAACGG TGGAAAAAAA ATGCCTGCAG TGCGTGTGCG
    TGTCATAGAG GGGCAGTCAG ATGTCTGAGA GAAACCTGTG ACTCTGTTAT TTGTGAGAAG
    GGGGATAACA AAGTGCAGCG TTCCGGGAAG TGCTGTGAGG AGTGTGTGTC TTCCAAGGAA
    AACTGCTTGT ATGATGGCAC TATCAGGTAC CACGGTGAGA TGTGGAATAT TACTCGCTGT
    GACTTCTGCA TGTGTGATGA AGGTCAAGTG ACTTGTCACA AAGCAGAGTG CGCCAAAGTA
    GAATGTGCCA AGGGTGAAGA ATTAATTCAC TTAGATGGGA AGTGCTGTCC TGAATGTATT
    TCAAGTAATC GTCATTGTTT TTACAAAGGA CATACCAAAG CTAGCGGAGA GACGTGGAAG
    GAAGGGCTGT GCAGAGAGTG TGAATGCCGC GACTCAGAGG TGGTCTGCTT TCAGCTTTCC
    TGTCCTCCCT GCCCACCGGG CAGCATGGCT GTCAGGGAGA AGGGTGATTG CTGTCCACGC
    TGCCAGCCAG GAAAGTGCCA CCCAGACTGT TTGACCTGCT CGCAGTCCTT TGATCACTGT
    ATTGCCTGCC GTGATGTGAA GAAGCAGCTG CAGAATGGGC GCTGTGTGGA GAGCTGTGGG
    TCAGGCTTCT ATCAGGATGG GGGGACTTGC TTAGCCTGCA ATGAAACATG CTCAGCCTGT
    ACGAATGGTT TTGAGTGTTC CTCATGCCAG ACGTCCCTGC TGATGAAGAA TGGGCAGTGT
    GTGACTTCAT GTGGGAAGGG ATTCTTTCAG GATCAACTCA TATGTGCAGT TTGTCATGAG
    TCCTGTTCCT CGTGTTGGGG ACCTGCTGAC AACAGTTGTC TCTCCTGCAA AGACACGTCA
    CATGTGCTAA AAGCAGGGCT CTGCGTGGCC AGCTGTGGAA AGGGATTTTA TGAGAAGGAT
    GGAATCTGCA GTGTTTGTGA CCCGTCCTGT GAAACGTGCA ATTCAGCCCA GCCCAGTTGT
    TTAACCTGTG CTTCAGATAA GGTGTTACAC AACGGGAACT GCATTTCAGA GTGCCCAGGC
    GGATATTTCC CAGGCAGCAA TGGAAGATGC AGGGCTTGTC ATGATTCGTG CTCCACCTGT
    GAGGGACCTC TGGCCACTCA CTGTACCTCC TGTTCCCTTC CTCTGGCTTT GCACCAGGGC
    CAGTGCCTGC AGGGCTGTGG AGAGGGCTTT TATCAGGATC ACAGCATTTG CAGGGGTTGC
    CACCCATCCT GCCGTAACTG CATTGGCCCA GGGGCCTCTC ATTGCCTGCA GTGCTGGGCA
    CCAGAGGATG TGCTGCAGCC CCATCAACCT GTGGAAGGAG CTGTGCATGG CTTTTGCCTC
    TCACACTGCC AGGCCCAGTT CTACCTGGAT GTCACAGGAG TCTGCAAACA GTGCCACTCC
    TCCTGTGCAG GCTGCATAGG GAACACTTCC CAGGACTGTA CGGCCTGTCC GTCTTCGCAC
    ATGCTGCTCG AGGGCAGATG CCTCTCCGAG TGTCCAGAGG GGTTGTTCAG CCAGCAGGAT
    CACTGCTCCT CCTGCCATCC GTCATGCAAG ACATGCCATG GCCCTTCTGA TTTGGAGTGC
    TTAACCTGCC ATCCCCATGC AACTCTGGCT GCTGGAAGGT GCAGGACTAG CTGCAAGGAA
    GAGCAGTATC TCAACTTGGT TGGATATTGC GTGGACTGCC ATCCTTTGTG CCATCAGTGT
    GTGGCCAACC TGTGGGACAC TGGCAGCCTC TGTCTCAAGT GCCAGAATGC CCGTCACTTG
    CTCCTTGGGG ATCACTGCGT TCCTGACTGT CCAGCAGGAT ACTACACAGA GAGCGGCGCC
    TGCAAACGAT GTCACCCCTC ATGTAAAACC TGCAGAGGTA GGGGTCCTCT TTCCTGCTCC
    TCCTGCAACA CCAGCCTGGT TCTGTCCCAC ACCAGCATGT GCGTTCCAGT CTGTTCTCCG
    GGATACTACA AGGATCAGAG CCAGACCTGC AAACCTTGCA ACAGCCAGTG CCTGAACTGT
    GAATCAGCTA CAGGCTGTAT GTCTTGCAGA GATCCAGCTA AGGTCCTGTT ATTTGGAGAA
    TGCCAGTATG AGAGCTGTGC TCAGCAGTAT TATCTGGATT TTTCAACCAA GACTTGCAAA
    GAGTGTGACT GGAGCTGCAA TGCTTGTAAA GGCCCTCTGA GGACCGATTG CCTGCAGTGT
    ATGGAGGGTA GTGTACTCCA CAAAGGAGCT TGTGTGGAAC AGTGCCCCAC AGCTTTCTAC
    AAGGAGTCAG ACATGTGCCA GAGGTGTGAT CAACATTGCC TTCAGTGTCA CCAAGCAAAT
    GAGTGCAGCC TTTGTGAAGC CCCATTTTTC CTCTTGGACA CTCAGTGCGT TTATAAGTGT
    GGGAAGGGAT ATTATGCAGA CCACGCACAG CGAAAATGCA TAGCTTGTCC TCACGGGTGC
    TTGGAGTGTG ACAGTGACGG CCTTTGCCAC CTCTGTGATG GCAACACCTT TCTGAAAAAC
    AAGATTTGCG TTTCTGCCTG TGGACAGGGA TTCTATGGCA ATAAGTGGAC CAGAGAGTGT
    GAAGTCAGCA GGCATCCTTC AAGCTTCCGT GTAAACAGTA CCCTCACAGT AGGGATTGGT
    GGGGTAAAGC CTCTAGACCT CTCCTTACTG GATGTGCGTG ATCTGGATGG TGACACAGGA
    CAGCTCTTGT TCCATATTGA CAGCGCTCCC TCCAATGGCA GGCTAGTGAT GGTGGTGAAT
    GGGAAAGAAG CGCAGCTGAG CAAAGGTGAC CACTTCAGCT TCAAGGATGT GAAGGAGAAT
    AGAGTCCGCT TTGTGCACAG CAGAGAGAAA TCCAGGAAAG GACAGCTTTC CTTGAGAGTC
    AGCGATCAAC AGTTCTCCCA TCCTGAGGTG ATCAACATTC AAGCATTTTC AACACAGGCC
    CCCTATCTGC TGAGAAATGA GGCCCTCCTT GTCCGCAGAG GGGAAATTGG AACTATAACA
    GACCTGTTGC TTGATGTGAG AGATGGTGAC AATCCTCAGG ATGTCATGTT GATGGTTTTA
    GAGCCTCCAC GTCATGGTCA GCTGATAAAA CCTCCCAGGG ACTCAGCTTC TTCAGTCTAC
    ATGTTCCACC TCGATGACCT TGCTGGTGGA CTGCTACGTT ATGCTCACGA TGGCTCTGAC
    ACCCAGGATG ATGCAATTCT TCTGCAAGTG AACGATGGAT ACCACTTCCA GAACATCCTG
    TTCCACATTA AGATCGCTCC CAAGAGTTAC AGAAGCCCTT GGCTGGTGAC AAATTCCCTG
    GTCTGGGTGC CCCAGGGAGG CATGCTGCAG ATCTCCAAGA CAATTCTGCA CGCTGAGGTA
    CCTGGAGCCC GTGACTCTGA GATAACCTAC AACATCATCA AGGACCAGCC AAGACATGGT
    GAAGTGGTGC TTCTGGTCCC AATGCCGGCA GATGGCCCAG CAGAGTCATG GCAGCTTTTG
    CCTGATGGAA GAGCGGCCTC ACCTACAACC TCTTTTACCC AGCAGGACAT CAATAATGGC
    ATCGTGTGGT ACAGGCACTC TGGAACTCAA GTAGAGAGTG ACTCCTTCCA GTTCCAGGTG
    TCCAGCTCTG CCAGTCCACA AGCCAGCTTG GAAAGCCACG TGTTCAACGT TGCTGTGCTC
    CCACAGACAC CCAGAGCACC TCAGCTTTCC CTTGGCTCCT CTTTGCACAT GGCTGTGCTG
    GAGGACCGCG TGACAGTGAT TGAGCCCCAT CACCTCTCCT TTGTAGACCC AGAGATTCCC
    AGTGAGAAAA TCCTGTTCAA CGTGACTGTC CCTCTGCTTC CTGGCCAAGG TATTGTGGAG
    CACAGAGACA GGCCTCACTC GCCAGTCAGG TATTTCACTC AAGCAGATAT AAACCATGGC
    AAGATTGCTT ATCGTCCACC AACTGCAGCT CCACACTTGA GAGAGATCAT TGCATTCTCA
    TTTGCAGGTC TCCCAGAGTC AGTGAACTTC CGATTCACAG TGTCTGGTGG AGAACACACA
    ACCCCGGAGA TGGCGTTTGT CATTCACTTG CTCTCTGCAG AGCAGCAGCC TCCAGTCTTC
    CAGATCACAG CTCCTTTCCT GGAGGTCAGC CAGGGGGGAA AGGCCACCAT CGGTGTCCAG
    TTGGCTGTGA GTGACACAGA TACAGCTCCT GAGGGCCTCT TCTTTGAGCT GGTGAAACCT
    CCCCATCATG GCATCGTGCT CAAGCATGAT GCAGGTGTGC AGGAGCACAT GAGAGCAGGT
    GACACCTTCA CCTATGAGGA CGTCACTCGA AATGCTCTGC AGTACGTACA TGATGGCTCA
    TCGGCAGCAG AAGACAGCAT GGAAATCTCT GTCACCGATG GCGTCACCAC AGTGACCACA
    GTGCTGAGAG TGGAGGTGTC CCTACTGGAT AACAACGGCC CACAGCTGGC CCCAGGCTGC
    CTGTTGGCAA TCACTGTGGC TAGTAAAAGC TCTGCAACTC TCTCTCGACT GCACCTCGCT
    TACATTGACA ACAATTCTCC TGACTCAGAG ATAAGAATCC AGTTAATATC TCTGCCCACG
    TACGGCACCC TCTTACGGAC GTCTGGCTCT CATGCTGAAG AACTTTCTAG GGTTTATAAT
    TTCACCATGG AAGACATCAA CAACCAGAGG ATCAGTTACT CCACTGTGTT TGAGACAAGC
    AATCAGCCAG TTACGGATGC CTTTCACTTC ACTGTTTTTG ATGCTGATAA TAACCGGCTT
    GACAGACAGA TGTTTACCAT CACCATCACG TCTGCCCAGA CAGCACCATC ACTCATTGTG
    TTTGCTGACC ATATCGCTGT GGATGAGGGG GGCAGGGTCC CACTTCTGGC TCATCACCAC
    TTAACTGCTG ATTATGATAC TGCTGCCAAG GAAGACCTGA GGGTCACGGT TATCACTCTA
    CCCGTGTATG GCTACATTGA AAACACTAAG ACAGGTGAGA CTTTTGGACC ACAGAGTGCT
    GTGACCACAG ATGCATCCTT CTCCATTCAA GATGTTCTAG AGAACAACAT TTACTACTTT
    CAGAGCGTCC ATGAAAGCAT CGAGCCAACA AGTGATGTTT TCAGCTTTTA CGTGAGCGAT
    GGCTTCAGCC AGTCTGAGGT GCACAGCATC AACATCACCA TTCAGCGACG GAATGATGAG
    CCCCCAAAGA TGGTGTTGGA GCCTGTCAGA GTGCATGAGA GCTCAGTGGT TGTTACCAGT
    GCCTCACTCA ACCTGCAGGA CCTGGACACC CAGAACAGTG AGCTTGTCAT CATGGTTACC
    AAAACTCCTG AGCACGGTCA CCTTCGCAGG AGGCAGACTG CTGTGGAGCC CCCAGAGCGT
    GGACACGTGC TGTCTCTGGG CTCATCCTTC ACCTACCAGG ACATTTTGGA TGAGCTGATT
    GTTTATTCTC AAAGCGGTGC AGCAGCACAG ACGGATGAGT TTGGCTTCTC CATCACAGAT
    GGTCTCTACA TGCAGACAGG GAAGCTGGAG TTCTCCATGG AGCTTCCAAA GAAGCAGCTG
    CCCACATTGA CTGCCAGCAG GGTCCTGCAG CTCCCCACTG GCTCTGCAGC ACACATCACA
    GATCAACACT TGAAAGCAGC AGCTGTTTAT CCAGATGACA CAATGATCAG ATTTGTCATG
    AAGAGGGATC CCAGCGTGGG CCATTTGCAG TTGACTGATA TTGATAATCC AGTCGAGATC
    TCTGTCAGAG GGCCTGTCAA AAGTTTTACC CAGGCTGATA TAAATAAAGG GCAAGTGGTT
    TATAGTCACA AGAAGGGGGA TCCTGGTGGA AATTTTGCCT TCACCTTTGA TGTGATCGAT
    GCAGATGGCA GCAGACTGAT GGACCAGTTT TTTCATATCA CTGTATTAGA AGACAGGTTC
    CCCCCCTCCA TCGTCGCTAA CACAGGGCTG GTCTTAGATG AGAACTCTGC AAAGAAGATC
    ACAACACTGC AGCTCTCTGC CACTGACCGG GACAGTGAGC CGACCCAGCT CCAGTACAAG
    CTCACCAAGC AGCCACAGCT GGGGCACCTG GAGCATGTGG CCTCCCCAGG GACAAAAATC
    AGTTCTTTCA GCCAGGCAGA CTTGGCCTCT CGCAGCATCC AATACGTTCA CACTAGCGAC
    ACTGAAAAGC ATACGGATGC CTTCACCTTC TCTGTGTCTG ATGGAACAAA TGAGGTCAGC
    CAGACATTTG ACATCACCAT AAACCCTGTG GATGATTCCT TACCTGTAGT GCAAAGCTTT
    GGGATGAGAG TTCAGGAAGG TGTGAGAAAA ACCATCACAG AGTTTGAGCT TAAAGCAACA
    GATGCTGACA CAAAGGCTGA GGCAATTACA TTCACAGTCG TGCAGCCCCC TCGACATGGC
    CTGATTGAAC ACACCAGCAA TGGGCAGCAT TACCACCAAG CCACCACCTT CACCATGGAT
    GACATTTACC AGAACCGCAT CAGCTACAGT CACGATGGAA GCAACTCACT GAAGGATCGC
    TTTGCTTTCA CTGTGTCTGA TGGGACCAAT CCCTTCTTCA TTATGGAGGA TGGGGGGAAA
    AAGGTTGTGA CAGCAGCACC ACAGCGCTTC AGAATGGACA TCCTCCCTGT GGATGATGGG
    ACACCCAGGG TGGTCACCAA CCAGGGTTTG CAATGGCTGG AGTATGTGGA TGGCAAGGCA
    ACCAATCTAA TCACCAAGAA GGAATTACTG ACAACAGATC CTGACACAGA TGACAAACAG
    CTGATCTATG AGATAACAGA TGGTCCCAGG AATGGTTATG TGGAGAACAA GCTGAAACCA
    GGAACAACTG TGACCATGTT TTCTCAGGAG GATGTGAATC AGGGCCTCAT TCGGTATGTG
    ATGCATGAGG AGAAGGTTCA GGAGACTATG GACAGTTTTC AGTTCCTCGT GAAGGACAGC
    AAACCCAATG TGGTCCGTGG CAACGTCTTC CACATCCAGT GGTCTCTCCT CAGCTTTACA
    TACAGCAGCT ACAACGTCAG TGAGAGCGCT GGCTCTGTGA GCGTCACCGT GCGGCGGATT
    GGTAACCTTA ACCAGTATGC CATTGTCTTG TGCCGCACGG AGCAGGGAAC AGCCACTTCC
    AGCTCTGGTT CACAGTCTGA AGAGCAGGAC TATGTGGAGT ACGCAGGGCA GGTGCAGTTT
    GAGGAGCGGG AAGACACCAA GGCCTGCACC ATCGTCATTA ACGATGACGA CATCTTTGAG
    AGCACCGAGA GCTTCACTGT GGAGCTCAGC ATGCCAGCCT ATGCTCTGCT GGGGAATGTC
    ACCCATGCCA CAGTCACCAT CACAGACGCT GAGGATGAGC CCATGCTGCA GTTCGACAGG
    AAGACGTACC ACGTCAGTGA GAGCGCTGGG TTTCTCTCTG CGCCTGTTGA AAGGAAAGGA
    GATGCTAGCA GCACTGTGTC TGCTGTCTGC TACACTGTCC CCAAATCAGC CAAGGGAAGC
    AGCCTTTATA TGCTGGAGTC TGGCTCCGAC TTCAAGTCCC GAGGGATGAC AGATGACAGC
    CGCATAGTTT TTGGACCAGG TGTTACCATG TCGACATGTG ATGTCATGCT TATTGATGAC
    AGTGAATATG AAGAAGAAGA GGAGTTTGAG ATAGTATTGG CTGATGCTTC GGAAAATGCA
    CGCATTGGCA ACCGGGCTTC AGCCAGCGTC TTCATCGGTG GTCCCAATGA TGCCTCGACA
    GTGTTCCTGG GAAATGCCAC GTTCACCGTC AGTGAGGCTG CAGGAAACAT TGAAATTCCA
    GTCCTCCGTC ACGGACCTGA TCTTTCAACC CCCACCTCAG TGTGGTGTGC TACTCGGACA
    TCAGATCCTC CATCTGCCAG CCCAGGAGTT GATTATGTCC CCAGCTCCAG AAAAATAGAG
    TTCAGGCCAG GCAAGACAGA AGAGTACCAA CCATGCAGTT CACCAAGGAT GTGTTCACAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015132104.1
    CDS1735..10317
    Translation

    Target ORF information:

    RefSeq Version XM_015276618.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015276618.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGGGCGCCT GGCTGCCGCT GTGCCTGCTC AGCGCCGCGC TGGCCGCCGC CGCCGCCGCC 
    GCCTCAGGTG CCTGTGTCTA TCAAGGCTTG TTGTTTGAGA ATAACACAAT TTGGAAGCCA
    GATTCTTGCC AAGACTGCAG TTGCCAAAGC AATGTTGTCA CCTGTGAACC TGCTGTCTGC
    GAACATCCTC AGTGTGACTT TGAGAAGGGA GAAGTGCTCC AAATAGCCCC AAACAAATGT
    TGCCCTAAAT GTGCCTCACA AACTGAAGGA TATTGCCAGC ATGAAGGACA GATCCACAGT
    CATGGCACGC AGTGGGCCAG CTCTGGATGT GTTCTGTGCT CCTGTGCCCA TGGGAAGGTG
    AGCTGCACGC CCATAGCCTG CCCAGTCCTC ACCTGTGAAA GGGATGAGCT GCCATACACT
    GCCCAGGGAG CCTGTTGTCC CACATGCGTG GGCCTTGGTG AGCCCTGCTA CTTTGATGGC
    CGTTCGTTCC GGGATGGTGA GGACTGGCGC CTTGGTCGGT GCTCCAAATG TGTCTGCAGG
    GATGGCGTGA CTCAGTGCTT CACAGCTTCC TGTGAGGCTG TTCTGTGCAA ACAGGATGAA
    GTTTTGGCTA TGTCTCCTGG AAAATGCTGT CCAGAATGTG TTCCAAAGTC CTGCTCGGTG
    TCAGGAAAGG TGTACCAGCA CGGAGAACGG TGGAAAAAAA ATGCCTGCAG TGCGTGTGCG
    TGTCATAGAG GGGCAGTCAG ATGTCTGAGA GAAACCTGTG ACTCTGTTAT TTGTGAGAAG
    GGGGATAACA AAGTGCAGCG TTCCGGGAAG TGCTGTGAGG AGTGTGTGTC TTCCAAGGAA
    AACTGCTTGT ATGATGGCAC TATCAGGTAC CACGGTGAGA TGTGGAATAT TACTCGCTGT
    GACTTCTGCA TGTGTGATGA AGGTCAAGTG ACTTGTCACA AAGCAGAGTG CGCCAAAGTA
    GAATGTGCCA AGGGTGAAGA ATTAATTCAC TTAGATGGGA AGTGCTGTCC TGAATGTATT
    TCAAGTAATC GTCATTGTTT TTACAAAGGA CATACCAAAG CTAGCGGAGA GACGTGGAAG
    GAAGGGCTGT GCAGAGAGTG TGAATGCCGC GACTCAGAGG TGGTCTGCTT TCAGCTTTCC
    TGTCCTCCCT GCCCACCGGG CAGCATGGCT GTCAGGGAGA AGGGTGATTG CTGTCCACGC
    TGCCAGCCAG GAAAGTGCCA CCCAGACTGT TTGACCTGCT CGCAGTCCTT TGATCACTGT
    ATTGCCTGCC GTGATGTGAA GAAGCAGCTG CAGAATGGGC GCTGTGTGGA GAGCTGTGGG
    TCAGGCTTCT ATCAGGATGG GGGGACTTGC TTAGCCTGCA ATGAAACATG CTCAGCCTGT
    ACGAATGGTT TTGAGTGTTC CTCATGCCAG ACGTCCCTGC TGATGAAGAA TGGGCAGTGT
    GTGACTTCAT GTGGGAAGGG ATTCTTTCAG GATCAACTCA TATGTGCAGT TTGTCATGAG
    TCCTGTTCCT CGTGTTGGGG ACCTGCTGAC AACAGTTGTC TCTCCTGCAA AGACACGTCA
    CATGTGCTAA AAGCAGGGCT CTGCGTGGCC AGCTGTGGAA AGGGATTTTA TGAGAAGGAT
    GGAATCTGCA GTGTTTGTGA CCCGTCCTGT GAAACGTGCA ATTCAGCCCA GCCCAGTTGT
    TTAACCTGTG CTTCAGATAA GGTGTTACAC AACGGGAACT GCATTTCAGA GTGCCCAGGC
    GGATATTTCC CAGGCAGCAA TGGAAGATGC AGGGCTTGTC ATGATTCGTG CTCCACCTGT
    GAGGGACCTC TGGCCACTCA CTGTACCTCC TGTTCCCTTC CTCTGGCTTT GCACCAGGGC
    CAGTGCCTGC AGGGCTGTGG AGAGGGCTTT TATCAGGATC ACAGCATTTG CAGGGGTTGC
    CACCCATCCT GCCGTAACTG CATTGGCCCA GGGGCCTCTC ATTGCCTGCA GTGCTGGGCA
    CCAGAGGATG TGCTGCAGCC CCATCAACCT GTGGAAGGAG CTGTGCATGG CTTTTGCCTC
    TCACACTGCC AGGCCCAGTT CTACCTGGAT GTCACAGGAG TCTGCAAACA GTGCCACTCC
    TCCTGTGCAG GCTGCATAGG GAACACTTCC CAGGACTGTA CGGCCTGTCC GTCTTCGCAC
    ATGCTGCTCG AGGGCAGATG CCTCTCCGAG TGTCCAGAGG GGTTGTTCAG CCAGCAGGAT
    CACTGCTCCT CCTGCCATCC GTCATGCAAG ACATGCCATG GCCCTTCTGA TTTGGAGTGC
    TTAACCTGCC ATCCCCATGC AACTCTGGCT GCTGGAAGGT GCAGGACTAG CTGCAAGGAA
    GAGCAGTATC TCAACTTGGT TGGATATTGC GTGGACTGCC ATCCTTTGTG CCATCAGTGT
    GTGGCCAACC TGTGGGACAC TGGCAGCCTC TGTCTCAAGT GCCAGAATGC CCGTCACTTG
    CTCCTTGGGG ATCACTGCGT TCCTGACTGT CCAGCAGGAT ACTACACAGA GAGCGGCGCC
    TGCAAACGAT GTCACCCCTC ATGTAAAACC TGCAGAGGTA GGGGTCCTCT TTCCTGCTCC
    TCCTGCAACA CCAGCCTGGT TCTGTCCCAC ACCAGCATGT GCGTTCCAGT CTGTTCTCCG
    GGATACTACA AGGATCAGAG CCAGACCTGC AAACCTTGCA ACAGCCAGTG CCTGAACTGT
    GAATCAGCTA CAGGCTGTAT GTCTTGCAGA GATCCAGCTA AGGTCCTGTT ATTTGGAGAA
    TGCCAGTATG AGAGCTGTGC TCAGCAGTAT TATCTGGATT TTTCAACCAA GACTTGCAAA
    GAGTGTGACT GGAGCTGCAA TGCTTGTAAA GGCCCTCTGA GGACCGATTG CCTGCAGTGT
    ATGGAGGGTA GTGTACTCCA CAAAGGAGCT TGTGTGGAAC AGTGCCCCAC AGCTTTCTAC
    AAGGAGTCAG ACATGTGCCA GAGGTGTGAT CAACATTGCC TTCAGTGTCA CCAAGCAAAT
    GAGTGCAGCC TTTGTGAAGC CCCATTTTTC CTCTTGGACA CTCAGTGCGT TTATAAGTGT
    GGGAAGGGAT ATTATGCAGA CCACGCACAG CGAAAATGCA TAGCTTGTCC TCACGGGTGC
    TTGGAGTGTG ACAGTGACGG CCTTTGCCAC CTCTGTGATG GCAACACCTT TCTGAAAAAC
    AAGATTTGCG TTTCTGCCTG TGGACAGGGA TTCTATGGCA ATAAGTGGAC CAGAGAGTGT
    GAAGTCAGCA GGCATCCTTC AAGCTTCCGT GTAAACAGTA CCCTCACAGT AGGGATTGGT
    GGGGTAAAGC CTCTAGACCT CTCCTTACTG GATGTGCGTG ATCTGGATGG TGACACAGGA
    CAGCTCTTGT TCCATATTGA CAGCGCTCCC TCCAATGGCA GGCTAGTGAT GGTGGTGAAT
    GGGAAAGAAG CGCAGCTGAG CAAAGGTGAC CACTTCAGCT TCAAGGATGT GAAGGAGAAT
    AGAGTCCGCT TTGTGCACAG CAGAGAGAAA TCCAGGAAAG GACAGCTTTC CTTGAGAGTC
    AGCGATCAAC AGTTCTCCCA TCCTGAGGTG ATCAACATTC AAGCATTTTC AACACAGGCC
    CCCTATCTGC TGAGAAATGA GGCCCTCCTT GTCCGCAGAG GGGAAATTGG AACTATAACA
    GACCTGTTGC TTGATGTGAG AGATGGTGAC AATCCTCAGG ATGTCATGTT GATGGTTTTA
    GAGCCTCCAC GTCATGGTCA GCTGATAAAA CCTCCCAGGG ACTCAGCTTC TTCAGTCTAC
    ATGTTCCACC TCGATGACCT TGCTGGTGGA CTGCTACGTT ATGCTCACGA TGGCTCTGAC
    ACCCAGGATG ATGCAATTCT TCTGCAAGTG AACGATGGAT ACCACTTCCA GAACATCCTG
    TTCCACATTA AGATCGCTCC CAAGAGTTAC AGAAGCCCTT GGCTGGTGAC AAATTCCCTG
    GTCTGGGTGC CCCAGGGAGG CATGCTGCAG ATCTCCAAGA CAATTCTGCA CGCTGAGGTA
    CCTGGAGCCC GTGACTCTGA GATAACCTAC AACATCATCA AGGACCAGCC AAGACATGGT
    GAAGTGGTGC TTCTGGTCCC AATGCCGGCA GATGGCCCAG CAGAGTCATG GCAGCTTTTG
    CCTGATGGAA GAGCGGCCTC ACCTACAACC TCTTTTACCC AGCAGGACAT CAATAATGGC
    ATCGTGTGGT ACAGGCACTC TGGAACTCAA GTAGAGAGTG ACTCCTTCCA GTTCCAGGTG
    TCCAGCTCTG CCAGTCCACA AGCCAGCTTG GAAAGCCACG TGTTCAACGT TGCTGTGCTC
    CCACAGACAC CCAGAGCACC TCAGCTTTCC CTTGGCTCCT CTTTGCACAT GGCTGTGCTG
    GAGGACCGCG TGACAGTGAT TGAGCCCCAT CACCTCTCCT TTGTAGACCC AGAGATTCCC
    AGTGAGAAAA TCCTGTTCAA CGTGACTGTC CCTCTGCTTC CTGGCCAAGG TATTGTGGAG
    CACAGAGACA GGCCTCACTC GCCAGTCAGG TATTTCACTC AAGCAGATAT AAACCATGGC
    AAGATTGCTT ATCGTCCACC AACTGCAGCT CCACACTTGA GAGAGATCAT TGCATTCTCA
    TTTGCAGGTC TCCCAGAGTC AGTGAACTTC CGATTCACAG TGTCTGGTGG AGAACACACA
    ACCCCGGAGA TGGCGTTTGT CATTCACTTG CTCTCTGCAG AGCAGCAGCC TCCAGTCTTC
    CAGATCACAG CTCCTTTCCT GGAGGTCAGC CAGGGGGGAA AGGCCACCAT CGGTGTCCAG
    TTGGCTGTGA GTGACACAGA TACAGCTCCT GAGGGCCTCT TCTTTGAGCT GGTGAAACCT
    CCCCATCATG GCATCGTGCT CAAGCATGAT GCAGGTGTGC AGGAGCACAT GAGAGCAGGT
    GACACCTTCA CCTATGAGGA CGTCACTCGA AATGCTCTGC AGTACGTACA TGATGGCTCA
    TCGGCAGCAG AAGACAGCAT GGAAATCTCT GTCACCGATG GCGTCACCAC AGTGACCACA
    GTGCTGAGAG TGGAGGTGTC CCTACTGGAT AACAACGGCC CACAGCTGGC CCCAGGCTGC
    CTGTTGGCAA TCACTGTGGC TAGTAAAAGC TCTGCAACTC TCTCTCGACT GCACCTCGCT
    TACATTGACA ACAATTCTCC TGACTCAGAG ATAAGAATCC AGTTAATATC TCTGCCCACG
    TACGGCACCC TCTTACGGAC GTCTGGCTCT CATGCTGAAG AACTTTCTAG GGTTTATAAT
    TTCACCATGG AAGACATCAA CAACCAGAGG ATCAGTTACT CCACTGTGTT TGAGACAAGC
    AATCAGCCAG TTACGGATGC CTTTCACTTC ACTGTTTTTG ATGCTGATAA TAACCGGCTT
    GACAGACAGA TGTTTACCAT CACCATCACG TCTGCCCAGA CAGCACCATC ACTCATTGTG
    TTTGCTGACC ATATCGCTGT GGATGAGGGG GGCAGGGTCC CACTTCTGGC TCATCACCAC
    TTAACTGCTG ATTATGATAC TGCTGCCAAG GAAGACCTGA GGGTCACGGT TATCACTCTA
    CCCGTGTATG GCTACATTGA AAACACTAAG ACAGGTGAGA CTTTTGGACC ACAGAGTGCT
    GTGACCACAG ATGCATCCTT CTCCATTCAA GATGTTCTAG AGAACAACAT TTACTACTTT
    CAGAGCGTCC ATGAAAGCAT CGAGCCAACA AGTGATGTTT TCAGCTTTTA CGTGAGCGAT
    GGCTTCAGCC AGTCTGAGGT GCACAGCATC AACATCACCA TTCAGCGACG GAATGATGAG
    CCCCCAAAGA TGGTGTTGGA GCCTGTCAGA GTGCATGAGA GCTCAGTGGT TGTTACCAGT
    GCCTCACTCA ACCTGCAGGA CCTGGACACC CAGAACAGTG AGCTTGTCAT CATGGTTACC
    AAAACTCCTG AGCACGGTCA CCTTCGCAGG AGGCAGACTG CTGTGGAGCC CCCAGAGCGT
    GGACACGTGC TGTCTCTGGG CTCATCCTTC ACCTACCAGG ACATTTTGGA TGAGCTGATT
    GTTTATTCTC AAAGCGGTGC AGCAGCACAG ACGGATGAGT TTGGCTTCTC CATCACAGAT
    GGTCTCTACA TGCAGACAGG GAAGCTGGAG TTCTCCATGG AGCTTCCAAA GAAGCAGCTG
    CCCACATTGA CTGCCAGCAG GGTCCTGCAG CTCCCCACTG GCTCTGCAGC ACACATCACA
    GATCAACACT TGAAAGCAGC AGCTGTTTAT CCAGATGACA CAATGATCAG ATTTGTCATG
    AAGAGGGATC CCAGCGTGGG CCATTTGCAG TTGACTGATA TTGATAATCC AGTCGAGATC
    TCTGTCAGAG GGCCTGTCAA AAGTTTTACC CAGGCTGATA TAAATAAAGG GCAAGTGGTT
    TATAGTCACA AGAAGGGGGA TCCTGGTGGA AATTTTGCCT TCACCTTTGA TGTGATCGAT
    GCAGATGGCA GCAGACTGAT GGACCAGTTT TTTCATATCA CTGTATTAGA AGACAGGTTC
    CCCCCCTCCA TCGTCGCTAA CACAGGGCTG GTCTTAGATG AGAACTCTGC AAAGAAGATC
    ACAACACTGC AGCTCTCTGC CACTGACCGG GACAGTGAGC CGACCCAGCT CCAGTACAAG
    CTCACCAAGC AGCCACAGCT GGGGCACCTG GAGCATGTGG CCTCCCCAGG GACAAAAATC
    AGTTCTTTCA GCCAGGCAGA CTTGGCCTCT CGCAGCATCC AATACGTTCA CACTAGCGAC
    ACTGAAAAGC ATACGGATGC CTTCACCTTC TCTGTGTCTG ATGGAACAAA TGAGGTCAGC
    CAGACATTTG ACATCACCAT AAACCCTGTG GATGATTCCT TACCTGTAGT GCAAAGCTTT
    GGGATGAGAG TTCAGGAAGG TGTGAGAAAA ACCATCACAG AGTTTGAGCT TAAAGCAACA
    GATGCTGACA CAAAGGCTGA GGCAATTACA TTCACAGTCG TGCAGCCCCC TCGACATGGC
    CTGATTGAAC ACACCAGCAA TGGGCAGCAT TACCACCAAG CCACCACCTT CACCATGGAT
    GACATTTACC AGAACCGCAT CAGCTACAGT CACGATGGAA GCAACTCACT GAAGGATCGC
    TTTGCTTTCA CTGTGTCTGA TGGGACCAAT CCCTTCTTCA TTATGGAGGA TGGGGGGAAA
    AAGGTTGTGA CAGCAGCACC ACAGCGCTTC AGAATGGACA TCCTCCCTGT GGATGATGGG
    ACACCCAGGG TGGTCACCAA CCAGGGTTTG CAATGGCTGG AGTATGTGGA TGGCAAGGCA
    ACCAATCTAA TCACCAAGAA GGAATTACTG ACAACAGATC CTGACACAGA TGACAAACAG
    CTGATCTATG AGATAACAGA TGGTCCCAGG AATGGTTATG TGGAGAACAA GCTGAAACCA
    GGAACAACTG TGACCATGTT TTCTCAGGAG GATGTGAATC AGGGCCTCAT TCGGTATGTG
    ATGCATGAGG AGAAGGTTCA GGAGACTATG GACAGTTTTC AGTTCCTCGT GAAGGACAGC
    AAACCCAATG TGGTCCGTGG CAACGTCTTC CACATCCAGT GGTCTCTCCT CAGCTTTACA
    TACAGCAGCT ACAACGTCAG TGAGAGCGCT GGCTCTGTGA GCGTCACCGT GCGGCGGATT
    GGTAACCTTA ACCAGTATGC CATTGTCTTG TGCCGCACGG AGCAGGGAAC AGCCACTTCC
    AGCTCTGGTT CACAGTCTGA AGAGCAGGAC TATGTGGAGT ACGCAGGGCA GGTGCAGTTT
    GAGGAGCGGG AAGACACCAA GGCCTGCACC ATCGTCATTA ACGATGACGA CATCTTTGAG
    AGCACCGAGA GCTTCACTGT GGAGCTCAGC ATGCCAGCCT ATGCTCTGCT GGGGAATGTC
    ACCCATGCCA CAGTCACCAT CACAGACGCT GAGGATGAGC CCATGCTGCA GTTCGACAGG
    AAGACGTACC ACGTCAGTGA GAGCGCTGGG TTTCTCTCTG CGCCTGTTGA AAGGAAAGGA
    GATGCTAGCA GCACTGTGTC TGCTGTCTGC TACACTGTCC CCAAATCAGC CAAGGGAAGC
    AGCCTTTATA TGCTGGAGTC TGGCTCCGAC TTCAAGTCCC GAGGGATGAC AGATGACAGC
    CGCATAGTTT TTGGACCAGG TGTTACCATG TCGACATGTG ATGTCATGCT TATTGATGAC
    AGTGAATATG AAGAAGAAGA GGAGTTTGAG ATAGTATTGG CTGATGCTTC GGAAAATGCA
    CGCATTGGCA ACCGGGCTTC AGCCAGCGTC TTCATCGGTG GTCCCAATGA TGCCTCGACA
    GTGTTCCTGG GAAATGCCAC GTTCACCGTC AGTGAGGCTG CAGGAAACAT TGAAATTCCA
    GTCCTCCGTC ACGGACCTGA TCTTTCAACC CCCACCTCAG TGTGGTGTGC TACTCGGACA
    TCAGATCCTC CATCTGCCAG CCCAGGAGTT GATTATGTCC CCAGCTCCAG AAAAATAGAG
    TTCAGGCCAG GCAAGACAGA AGAGTACCAA CCATGCAGTT CACCAAGGAT GTGTTCACAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35354
    Clone ID Related Accession (Same CDS sequence) XM_015276618.1 , XM_015276618.2
    Accession Version XM_015276618.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8583bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product extracellular matrix protein FRAS1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276618.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGGGCGCCT GGCTGCCGCT GTGCCTGCTC AGCGCCGCGC TGGCCGCCGC CGCCGCCGCC 
    GCCTCAGGTG CCTGTGTCTA TCAAGGCTTG TTGTTTGAGA ATAACACAAT TTGGAAGCCA
    GATTCTTGCC AAGACTGCAG TTGCCAAAGC AATGTTGTCA CCTGTGAACC TGCTGTCTGC
    GAACATCCTC AGTGTGACTT TGAGAAGGGA GAAGTGCTCC AAATAGCCCC AAACAAATGT
    TGCCCTAAAT GTGCCTCACA AACTGAAGGA TATTGCCAGC ATGAAGGACA GATCCACAGT
    CATGGCACGC AGTGGGCCAG CTCTGGATGT GTTCTGTGCT CCTGTGCCCA TGGGAAGGTG
    AGCTGCACGC CCATAGCCTG CCCAGTCCTC ACCTGTGAAA GGGATGAGCT GCCATACACT
    GCCCAGGGAG CCTGTTGTCC CACATGCGTG GGCCTTGGTG AGCCCTGCTA CTTTGATGGC
    CGTTCGTTCC GGGATGGTGA GGACTGGCGC CTTGGTCGGT GCTCCAAATG TGTCTGCAGG
    GATGGCGTGA CTCAGTGCTT CACAGCTTCC TGTGAGGCTG TTCTGTGCAA ACAGGATGAA
    GTTTTGGCTA TGTCTCCTGG AAAATGCTGT CCAGAATGTG TTCCAAAGTC CTGCTCGGTG
    TCAGGAAAGG TGTACCAGCA CGGAGAACGG TGGAAAAAAA ATGCCTGCAG TGCGTGTGCG
    TGTCATAGAG GGGCAGTCAG ATGTCTGAGA GAAACCTGTG ACTCTGTTAT TTGTGAGAAG
    GGGGATAACA AAGTGCAGCG TTCCGGGAAG TGCTGTGAGG AGTGTGTGTC TTCCAAGGAA
    AACTGCTTGT ATGATGGCAC TATCAGGTAC CACGGTGAGA TGTGGAATAT TACTCGCTGT
    GACTTCTGCA TGTGTGATGA AGGTCAAGTG ACTTGTCACA AAGCAGAGTG CGCCAAAGTA
    GAATGTGCCA AGGGTGAAGA ATTAATTCAC TTAGATGGGA AGTGCTGTCC TGAATGTATT
    TCAAGTAATC GTCATTGTTT TTACAAAGGA CATACCAAAG CTAGCGGAGA GACGTGGAAG
    GAAGGGCTGT GCAGAGAGTG TGAATGCCGC GACTCAGAGG TGGTCTGCTT TCAGCTTTCC
    TGTCCTCCCT GCCCACCGGG CAGCATGGCT GTCAGGGAGA AGGGTGATTG CTGTCCACGC
    TGCCAGCCAG GAAAGTGCCA CCCAGACTGT TTGACCTGCT CGCAGTCCTT TGATCACTGT
    ATTGCCTGCC GTGATGTGAA GAAGCAGCTG CAGAATGGGC GCTGTGTGGA GAGCTGTGGG
    TCAGGCTTCT ATCAGGATGG GGGGACTTGC TTAGCCTGCA ATGAAACATG CTCAGCCTGT
    ACGAATGGTT TTGAGTGTTC CTCATGCCAG ACGTCCCTGC TGATGAAGAA TGGGCAGTGT
    GTGACTTCAT GTGGGAAGGG ATTCTTTCAG GATCAACTCA TATGTGCAGT TTGTCATGAG
    TCCTGTTCCT CGTGTTGGGG ACCTGCTGAC AACAGTTGTC TCTCCTGCAA AGACACGTCA
    CATGTGCTAA AAGCAGGGCT CTGCGTGGCC AGCTGTGGAA AGGGATTTTA TGAGAAGGAT
    GGAATCTGCA GTGTTTGTGA CCCGTCCTGT GAAACGTGCA ATTCAGCCCA GCCCAGTTGT
    TTAACCTGTG CTTCAGATAA GGTGTTACAC AACGGGAACT GCATTTCAGA GTGCCCAGGC
    GGATATTTCC CAGGCAGCAA TGGAAGATGC AGGGCTTGTC ATGATTCGTG CTCCACCTGT
    GAGGGACCTC TGGCCACTCA CTGTACCTCC TGTTCCCTTC CTCTGGCTTT GCACCAGGGC
    CAGTGCCTGC AGGGCTGTGG AGAGGGCTTT TATCAGGATC ACAGCATTTG CAGGGGTTGC
    CACCCATCCT GCCGTAACTG CATTGGCCCA GGGGCCTCTC ATTGCCTGCA GTGCTGGGCA
    CCAGAGGATG TGCTGCAGCC CCATCAACCT GTGGAAGGAG CTGTGCATGG CTTTTGCCTC
    TCACACTGCC AGGCCCAGTT CTACCTGGAT GTCACAGGAG TCTGCAAACA GTGCCACTCC
    TCCTGTGCAG GCTGCATAGG GAACACTTCC CAGGACTGTA CGGCCTGTCC GTCTTCGCAC
    ATGCTGCTCG AGGGCAGATG CCTCTCCGAG TGTCCAGAGG GGTTGTTCAG CCAGCAGGAT
    CACTGCTCCT CCTGCCATCC GTCATGCAAG ACATGCCATG GCCCTTCTGA TTTGGAGTGC
    TTAACCTGCC ATCCCCATGC AACTCTGGCT GCTGGAAGGT GCAGGACTAG CTGCAAGGAA
    GAGCAGTATC TCAACTTGGT TGGATATTGC GTGGACTGCC ATCCTTTGTG CCATCAGTGT
    GTGGCCAACC TGTGGGACAC TGGCAGCCTC TGTCTCAAGT GCCAGAATGC CCGTCACTTG
    CTCCTTGGGG ATCACTGCGT TCCTGACTGT CCAGCAGGAT ACTACACAGA GAGCGGCGCC
    TGCAAACGAT GTCACCCCTC ATGTAAAACC TGCAGAGGTA GGGGTCCTCT TTCCTGCTCC
    TCCTGCAACA CCAGCCTGGT TCTGTCCCAC ACCAGCATGT GCGTTCCAGT CTGTTCTCCG
    GGATACTACA AGGATCAGAG CCAGACCTGC AAACCTTGCA ACAGCCAGTG CCTGAACTGT
    GAATCAGCTA CAGGCTGTAT GTCTTGCAGA GATCCAGCTA AGGTCCTGTT ATTTGGAGAA
    TGCCAGTATG AGAGCTGTGC TCAGCAGTAT TATCTGGATT TTTCAACCAA GACTTGCAAA
    GAGTGTGACT GGAGCTGCAA TGCTTGTAAA GGCCCTCTGA GGACCGATTG CCTGCAGTGT
    ATGGAGGGTA GTGTACTCCA CAAAGGAGCT TGTGTGGAAC AGTGCCCCAC AGCTTTCTAC
    AAGGAGTCAG ACATGTGCCA GAGGTGTGAT CAACATTGCC TTCAGTGTCA CCAAGCAAAT
    GAGTGCAGCC TTTGTGAAGC CCCATTTTTC CTCTTGGACA CTCAGTGCGT TTATAAGTGT
    GGGAAGGGAT ATTATGCAGA CCACGCACAG CGAAAATGCA TAGCTTGTCC TCACGGGTGC
    TTGGAGTGTG ACAGTGACGG CCTTTGCCAC CTCTGTGATG GCAACACCTT TCTGAAAAAC
    AAGATTTGCG TTTCTGCCTG TGGACAGGGA TTCTATGGCA ATAAGTGGAC CAGAGAGTGT
    GAAGTCAGCA GGCATCCTTC AAGCTTCCGT GTAAACAGTA CCCTCACAGT AGGGATTGGT
    GGGGTAAAGC CTCTAGACCT CTCCTTACTG GATGTGCGTG ATCTGGATGG TGACACAGGA
    CAGCTCTTGT TCCATATTGA CAGCGCTCCC TCCAATGGCA GGCTAGTGAT GGTGGTGAAT
    GGGAAAGAAG CGCAGCTGAG CAAAGGTGAC CACTTCAGCT TCAAGGATGT GAAGGAGAAT
    AGAGTCCGCT TTGTGCACAG CAGAGAGAAA TCCAGGAAAG GACAGCTTTC CTTGAGAGTC
    AGCGATCAAC AGTTCTCCCA TCCTGAGGTG ATCAACATTC AAGCATTTTC AACACAGGCC
    CCCTATCTGC TGAGAAATGA GGCCCTCCTT GTCCGCAGAG GGGAAATTGG AACTATAACA
    GACCTGTTGC TTGATGTGAG AGATGGTGAC AATCCTCAGG ATGTCATGTT GATGGTTTTA
    GAGCCTCCAC GTCATGGTCA GCTGATAAAA CCTCCCAGGG ACTCAGCTTC TTCAGTCTAC
    ATGTTCCACC TCGATGACCT TGCTGGTGGA CTGCTACGTT ATGCTCACGA TGGCTCTGAC
    ACCCAGGATG ATGCAATTCT TCTGCAAGTG AACGATGGAT ACCACTTCCA GAACATCCTG
    TTCCACATTA AGATCGCTCC CAAGAGTTAC AGAAGCCCTT GGCTGGTGAC AAATTCCCTG
    GTCTGGGTGC CCCAGGGAGG CATGCTGCAG ATCTCCAAGA CAATTCTGCA CGCTGAGGTA
    CCTGGAGCCC GTGACTCTGA GATAACCTAC AACATCATCA AGGACCAGCC AAGACATGGT
    GAAGTGGTGC TTCTGGTCCC AATGCCGGCA GATGGCCCAG CAGAGTCATG GCAGCTTTTG
    CCTGATGGAA GAGCGGCCTC ACCTACAACC TCTTTTACCC AGCAGGACAT CAATAATGGC
    ATCGTGTGGT ACAGGCACTC TGGAACTCAA GTAGAGAGTG ACTCCTTCCA GTTCCAGGTG
    TCCAGCTCTG CCAGTCCACA AGCCAGCTTG GAAAGCCACG TGTTCAACGT TGCTGTGCTC
    CCACAGACAC CCAGAGCACC TCAGCTTTCC CTTGGCTCCT CTTTGCACAT GGCTGTGCTG
    GAGGACCGCG TGACAGTGAT TGAGCCCCAT CACCTCTCCT TTGTAGACCC AGAGATTCCC
    AGTGAGAAAA TCCTGTTCAA CGTGACTGTC CCTCTGCTTC CTGGCCAAGG TATTGTGGAG
    CACAGAGACA GGCCTCACTC GCCAGTCAGG TATTTCACTC AAGCAGATAT AAACCATGGC
    AAGATTGCTT ATCGTCCACC AACTGCAGCT CCACACTTGA GAGAGATCAT TGCATTCTCA
    TTTGCAGGTC TCCCAGAGTC AGTGAACTTC CGATTCACAG TGTCTGGTGG AGAACACACA
    ACCCCGGAGA TGGCGTTTGT CATTCACTTG CTCTCTGCAG AGCAGCAGCC TCCAGTCTTC
    CAGATCACAG CTCCTTTCCT GGAGGTCAGC CAGGGGGGAA AGGCCACCAT CGGTGTCCAG
    TTGGCTGTGA GTGACACAGA TACAGCTCCT GAGGGCCTCT TCTTTGAGCT GGTGAAACCT
    CCCCATCATG GCATCGTGCT CAAGCATGAT GCAGGTGTGC AGGAGCACAT GAGAGCAGGT
    GACACCTTCA CCTATGAGGA CGTCACTCGA AATGCTCTGC AGTACGTACA TGATGGCTCA
    TCGGCAGCAG AAGACAGCAT GGAAATCTCT GTCACCGATG GCGTCACCAC AGTGACCACA
    GTGCTGAGAG TGGAGGTGTC CCTACTGGAT AACAACGGCC CACAGCTGGC CCCAGGCTGC
    CTGTTGGCAA TCACTGTGGC TAGTAAAAGC TCTGCAACTC TCTCTCGACT GCACCTCGCT
    TACATTGACA ACAATTCTCC TGACTCAGAG ATAAGAATCC AGTTAATATC TCTGCCCACG
    TACGGCACCC TCTTACGGAC GTCTGGCTCT CATGCTGAAG AACTTTCTAG GGTTTATAAT
    TTCACCATGG AAGACATCAA CAACCAGAGG ATCAGTTACT CCACTGTGTT TGAGACAAGC
    AATCAGCCAG TTACGGATGC CTTTCACTTC ACTGTTTTTG ATGCTGATAA TAACCGGCTT
    GACAGACAGA TGTTTACCAT CACCATCACG TCTGCCCAGA CAGCACCATC ACTCATTGTG
    TTTGCTGACC ATATCGCTGT GGATGAGGGG GGCAGGGTCC CACTTCTGGC TCATCACCAC
    TTAACTGCTG ATTATGATAC TGCTGCCAAG GAAGACCTGA GGGTCACGGT TATCACTCTA
    CCCGTGTATG GCTACATTGA AAACACTAAG ACAGGTGAGA CTTTTGGACC ACAGAGTGCT
    GTGACCACAG ATGCATCCTT CTCCATTCAA GATGTTCTAG AGAACAACAT TTACTACTTT
    CAGAGCGTCC ATGAAAGCAT CGAGCCAACA AGTGATGTTT TCAGCTTTTA CGTGAGCGAT
    GGCTTCAGCC AGTCTGAGGT GCACAGCATC AACATCACCA TTCAGCGACG GAATGATGAG
    CCCCCAAAGA TGGTGTTGGA GCCTGTCAGA GTGCATGAGA GCTCAGTGGT TGTTACCAGT
    GCCTCACTCA ACCTGCAGGA CCTGGACACC CAGAACAGTG AGCTTGTCAT CATGGTTACC
    AAAACTCCTG AGCACGGTCA CCTTCGCAGG AGGCAGACTG CTGTGGAGCC CCCAGAGCGT
    GGACACGTGC TGTCTCTGGG CTCATCCTTC ACCTACCAGG ACATTTTGGA TGAGCTGATT
    GTTTATTCTC AAAGCGGTGC AGCAGCACAG ACGGATGAGT TTGGCTTCTC CATCACAGAT
    GGTCTCTACA TGCAGACAGG GAAGCTGGAG TTCTCCATGG AGCTTCCAAA GAAGCAGCTG
    CCCACATTGA CTGCCAGCAG GGTCCTGCAG CTCCCCACTG GCTCTGCAGC ACACATCACA
    GATCAACACT TGAAAGCAGC AGCTGTTTAT CCAGATGACA CAATGATCAG ATTTGTCATG
    AAGAGGGATC CCAGCGTGGG CCATTTGCAG TTGACTGATA TTGATAATCC AGTCGAGATC
    TCTGTCAGAG GGCCTGTCAA AAGTTTTACC CAGGCTGATA TAAATAAAGG GCAAGTGGTT
    TATAGTCACA AGAAGGGGGA TCCTGGTGGA AATTTTGCCT TCACCTTTGA TGTGATCGAT
    GCAGATGGCA GCAGACTGAT GGACCAGTTT TTTCATATCA CTGTATTAGA AGACAGGTTC
    CCCCCCTCCA TCGTCGCTAA CACAGGGCTG GTCTTAGATG AGAACTCTGC AAAGAAGATC
    ACAACACTGC AGCTCTCTGC CACTGACCGG GACAGTGAGC CGACCCAGCT CCAGTACAAG
    CTCACCAAGC AGCCACAGCT GGGGCACCTG GAGCATGTGG CCTCCCCAGG GACAAAAATC
    AGTTCTTTCA GCCAGGCAGA CTTGGCCTCT CGCAGCATCC AATACGTTCA CACTAGCGAC
    ACTGAAAAGC ATACGGATGC CTTCACCTTC TCTGTGTCTG ATGGAACAAA TGAGGTCAGC
    CAGACATTTG ACATCACCAT AAACCCTGTG GATGATTCCT TACCTGTAGT GCAAAGCTTT
    GGGATGAGAG TTCAGGAAGG TGTGAGAAAA ACCATCACAG AGTTTGAGCT TAAAGCAACA
    GATGCTGACA CAAAGGCTGA GGCAATTACA TTCACAGTCG TGCAGCCCCC TCGACATGGC
    CTGATTGAAC ACACCAGCAA TGGGCAGCAT TACCACCAAG CCACCACCTT CACCATGGAT
    GACATTTACC AGAACCGCAT CAGCTACAGT CACGATGGAA GCAACTCACT GAAGGATCGC
    TTTGCTTTCA CTGTGTCTGA TGGGACCAAT CCCTTCTTCA TTATGGAGGA TGGGGGGAAA
    AAGGTTGTGA CAGCAGCACC ACAGCGCTTC AGAATGGACA TCCTCCCTGT GGATGATGGG
    ACACCCAGGG TGGTCACCAA CCAGGGTTTG CAATGGCTGG AGTATGTGGA TGGCAAGGCA
    ACCAATCTAA TCACCAAGAA GGAATTACTG ACAACAGATC CTGACACAGA TGACAAACAG
    CTGATCTATG AGATAACAGA TGGTCCCAGG AATGGTTATG TGGAGAACAA GCTGAAACCA
    GGAACAACTG TGACCATGTT TTCTCAGGAG GATGTGAATC AGGGCCTCAT TCGGTATGTG
    ATGCATGAGG AGAAGGTTCA GGAGACTATG GACAGTTTTC AGTTCCTCGT GAAGGACAGC
    AAACCCAATG TGGTCCGTGG CAACGTCTTC CACATCCAGT GGTCTCTCCT CAGCTTTACA
    TACAGCAGCT ACAACGTCAG TGAGAGCGCT GGCTCTGTGA GCGTCACCGT GCGGCGGATT
    GGTAACCTTA ACCAGTATGC CATTGTCTTG TGCCGCACGG AGCAGGGAAC AGCCACTTCC
    AGCTCTGGTT CACAGTCTGA AGAGCAGGAC TATGTGGAGT ACGCAGGGCA GGTGCAGTTT
    GAGGAGCGGG AAGACACCAA GGCCTGCACC ATCGTCATTA ACGATGACGA CATCTTTGAG
    AGCACCGAGA GCTTCACTGT GGAGCTCAGC ATGCCAGCCT ATGCTCTGCT GGGGAATGTC
    ACCCATGCCA CAGTCACCAT CACAGACGCT GAGGATGAGC CCATGCTGCA GTTCGACAGG
    AAGACGTACC ACGTCAGTGA GAGCGCTGGG TTTCTCTCTG CGCCTGTTGA AAGGAAAGGA
    GATGCTAGCA GCACTGTGTC TGCTGTCTGC TACACTGTCC CCAAATCAGC CAAGGGAAGC
    AGCCTTTATA TGCTGGAGTC TGGCTCCGAC TTCAAGTCCC GAGGGATGAC AGATGACAGC
    CGCATAGTTT TTGGACCAGG TGTTACCATG TCGACATGTG ATGTCATGCT TATTGATGAC
    AGTGAATATG AAGAAGAAGA GGAGTTTGAG ATAGTATTGG CTGATGCTTC GGAAAATGCA
    CGCATTGGCA ACCGGGCTTC AGCCAGCGTC TTCATCGGTG GTCCCAATGA TGCCTCGACA
    GTGTTCCTGG GAAATGCCAC GTTCACCGTC AGTGAGGCTG CAGGAAACAT TGAAATTCCA
    GTCCTCCGTC ACGGACCTGA TCTTTCAACC CCCACCTCAG TGTGGTGTGC TACTCGGACA
    TCAGATCCTC CATCTGCCAG CCCAGGAGTT GATTATGTCC CCAGCTCCAG AAAAATAGAG
    TTCAGGCCAG GCAAGACAGA AGAGTACCAA CCATGCAGTT CACCAAGGAT GTGTTCACAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015132104.1
    CDS476..9058
    Translation

    Target ORF information:

    RefSeq Version XM_015276618.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015276618.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGGGCGCCT GGCTGCCGCT GTGCCTGCTC AGCGCCGCGC TGGCCGCCGC CGCCGCCGCC 
    GCCTCAGGTG CCTGTGTCTA TCAAGGCTTG TTGTTTGAGA ATAACACAAT TTGGAAGCCA
    GATTCTTGCC AAGACTGCAG TTGCCAAAGC AATGTTGTCA CCTGTGAACC TGCTGTCTGC
    GAACATCCTC AGTGTGACTT TGAGAAGGGA GAAGTGCTCC AAATAGCCCC AAACAAATGT
    TGCCCTAAAT GTGCCTCACA AACTGAAGGA TATTGCCAGC ATGAAGGACA GATCCACAGT
    CATGGCACGC AGTGGGCCAG CTCTGGATGT GTTCTGTGCT CCTGTGCCCA TGGGAAGGTG
    AGCTGCACGC CCATAGCCTG CCCAGTCCTC ACCTGTGAAA GGGATGAGCT GCCATACACT
    GCCCAGGGAG CCTGTTGTCC CACATGCGTG GGCCTTGGTG AGCCCTGCTA CTTTGATGGC
    CGTTCGTTCC GGGATGGTGA GGACTGGCGC CTTGGTCGGT GCTCCAAATG TGTCTGCAGG
    GATGGCGTGA CTCAGTGCTT CACAGCTTCC TGTGAGGCTG TTCTGTGCAA ACAGGATGAA
    GTTTTGGCTA TGTCTCCTGG AAAATGCTGT CCAGAATGTG TTCCAAAGTC CTGCTCGGTG
    TCAGGAAAGG TGTACCAGCA CGGAGAACGG TGGAAAAAAA ATGCCTGCAG TGCGTGTGCG
    TGTCATAGAG GGGCAGTCAG ATGTCTGAGA GAAACCTGTG ACTCTGTTAT TTGTGAGAAG
    GGGGATAACA AAGTGCAGCG TTCCGGGAAG TGCTGTGAGG AGTGTGTGTC TTCCAAGGAA
    AACTGCTTGT ATGATGGCAC TATCAGGTAC CACGGTGAGA TGTGGAATAT TACTCGCTGT
    GACTTCTGCA TGTGTGATGA AGGTCAAGTG ACTTGTCACA AAGCAGAGTG CGCCAAAGTA
    GAATGTGCCA AGGGTGAAGA ATTAATTCAC TTAGATGGGA AGTGCTGTCC TGAATGTATT
    TCAAGTAATC GTCATTGTTT TTACAAAGGA CATACCAAAG CTAGCGGAGA GACGTGGAAG
    GAAGGGCTGT GCAGAGAGTG TGAATGCCGC GACTCAGAGG TGGTCTGCTT TCAGCTTTCC
    TGTCCTCCCT GCCCACCGGG CAGCATGGCT GTCAGGGAGA AGGGTGATTG CTGTCCACGC
    TGCCAGCCAG GAAAGTGCCA CCCAGACTGT TTGACCTGCT CGCAGTCCTT TGATCACTGT
    ATTGCCTGCC GTGATGTGAA GAAGCAGCTG CAGAATGGGC GCTGTGTGGA GAGCTGTGGG
    TCAGGCTTCT ATCAGGATGG GGGGACTTGC TTAGCCTGCA ATGAAACATG CTCAGCCTGT
    ACGAATGGTT TTGAGTGTTC CTCATGCCAG ACGTCCCTGC TGATGAAGAA TGGGCAGTGT
    GTGACTTCAT GTGGGAAGGG ATTCTTTCAG GATCAACTCA TATGTGCAGT TTGTCATGAG
    TCCTGTTCCT CGTGTTGGGG ACCTGCTGAC AACAGTTGTC TCTCCTGCAA AGACACGTCA
    CATGTGCTAA AAGCAGGGCT CTGCGTGGCC AGCTGTGGAA AGGGATTTTA TGAGAAGGAT
    GGAATCTGCA GTGTTTGTGA CCCGTCCTGT GAAACGTGCA ATTCAGCCCA GCCCAGTTGT
    TTAACCTGTG CTTCAGATAA GGTGTTACAC AACGGGAACT GCATTTCAGA GTGCCCAGGC
    GGATATTTCC CAGGCAGCAA TGGAAGATGC AGGGCTTGTC ATGATTCGTG CTCCACCTGT
    GAGGGACCTC TGGCCACTCA CTGTACCTCC TGTTCCCTTC CTCTGGCTTT GCACCAGGGC
    CAGTGCCTGC AGGGCTGTGG AGAGGGCTTT TATCAGGATC ACAGCATTTG CAGGGGTTGC
    CACCCATCCT GCCGTAACTG CATTGGCCCA GGGGCCTCTC ATTGCCTGCA GTGCTGGGCA
    CCAGAGGATG TGCTGCAGCC CCATCAACCT GTGGAAGGAG CTGTGCATGG CTTTTGCCTC
    TCACACTGCC AGGCCCAGTT CTACCTGGAT GTCACAGGAG TCTGCAAACA GTGCCACTCC
    TCCTGTGCAG GCTGCATAGG GAACACTTCC CAGGACTGTA CGGCCTGTCC GTCTTCGCAC
    ATGCTGCTCG AGGGCAGATG CCTCTCCGAG TGTCCAGAGG GGTTGTTCAG CCAGCAGGAT
    CACTGCTCCT CCTGCCATCC GTCATGCAAG ACATGCCATG GCCCTTCTGA TTTGGAGTGC
    TTAACCTGCC ATCCCCATGC AACTCTGGCT GCTGGAAGGT GCAGGACTAG CTGCAAGGAA
    GAGCAGTATC TCAACTTGGT TGGATATTGC GTGGACTGCC ATCCTTTGTG CCATCAGTGT
    GTGGCCAACC TGTGGGACAC TGGCAGCCTC TGTCTCAAGT GCCAGAATGC CCGTCACTTG
    CTCCTTGGGG ATCACTGCGT TCCTGACTGT CCAGCAGGAT ACTACACAGA GAGCGGCGCC
    TGCAAACGAT GTCACCCCTC ATGTAAAACC TGCAGAGGTA GGGGTCCTCT TTCCTGCTCC
    TCCTGCAACA CCAGCCTGGT TCTGTCCCAC ACCAGCATGT GCGTTCCAGT CTGTTCTCCG
    GGATACTACA AGGATCAGAG CCAGACCTGC AAACCTTGCA ACAGCCAGTG CCTGAACTGT
    GAATCAGCTA CAGGCTGTAT GTCTTGCAGA GATCCAGCTA AGGTCCTGTT ATTTGGAGAA
    TGCCAGTATG AGAGCTGTGC TCAGCAGTAT TATCTGGATT TTTCAACCAA GACTTGCAAA
    GAGTGTGACT GGAGCTGCAA TGCTTGTAAA GGCCCTCTGA GGACCGATTG CCTGCAGTGT
    ATGGAGGGTA GTGTACTCCA CAAAGGAGCT TGTGTGGAAC AGTGCCCCAC AGCTTTCTAC
    AAGGAGTCAG ACATGTGCCA GAGGTGTGAT CAACATTGCC TTCAGTGTCA CCAAGCAAAT
    GAGTGCAGCC TTTGTGAAGC CCCATTTTTC CTCTTGGACA CTCAGTGCGT TTATAAGTGT
    GGGAAGGGAT ATTATGCAGA CCACGCACAG CGAAAATGCA TAGCTTGTCC TCACGGGTGC
    TTGGAGTGTG ACAGTGACGG CCTTTGCCAC CTCTGTGATG GCAACACCTT TCTGAAAAAC
    AAGATTTGCG TTTCTGCCTG TGGACAGGGA TTCTATGGCA ATAAGTGGAC CAGAGAGTGT
    GAAGTCAGCA GGCATCCTTC AAGCTTCCGT GTAAACAGTA CCCTCACAGT AGGGATTGGT
    GGGGTAAAGC CTCTAGACCT CTCCTTACTG GATGTGCGTG ATCTGGATGG TGACACAGGA
    CAGCTCTTGT TCCATATTGA CAGCGCTCCC TCCAATGGCA GGCTAGTGAT GGTGGTGAAT
    GGGAAAGAAG CGCAGCTGAG CAAAGGTGAC CACTTCAGCT TCAAGGATGT GAAGGAGAAT
    AGAGTCCGCT TTGTGCACAG CAGAGAGAAA TCCAGGAAAG GACAGCTTTC CTTGAGAGTC
    AGCGATCAAC AGTTCTCCCA TCCTGAGGTG ATCAACATTC AAGCATTTTC AACACAGGCC
    CCCTATCTGC TGAGAAATGA GGCCCTCCTT GTCCGCAGAG GGGAAATTGG AACTATAACA
    GACCTGTTGC TTGATGTGAG AGATGGTGAC AATCCTCAGG ATGTCATGTT GATGGTTTTA
    GAGCCTCCAC GTCATGGTCA GCTGATAAAA CCTCCCAGGG ACTCAGCTTC TTCAGTCTAC
    ATGTTCCACC TCGATGACCT TGCTGGTGGA CTGCTACGTT ATGCTCACGA TGGCTCTGAC
    ACCCAGGATG ATGCAATTCT TCTGCAAGTG AACGATGGAT ACCACTTCCA GAACATCCTG
    TTCCACATTA AGATCGCTCC CAAGAGTTAC AGAAGCCCTT GGCTGGTGAC AAATTCCCTG
    GTCTGGGTGC CCCAGGGAGG CATGCTGCAG ATCTCCAAGA CAATTCTGCA CGCTGAGGTA
    CCTGGAGCCC GTGACTCTGA GATAACCTAC AACATCATCA AGGACCAGCC AAGACATGGT
    GAAGTGGTGC TTCTGGTCCC AATGCCGGCA GATGGCCCAG CAGAGTCATG GCAGCTTTTG
    CCTGATGGAA GAGCGGCCTC ACCTACAACC TCTTTTACCC AGCAGGACAT CAATAATGGC
    ATCGTGTGGT ACAGGCACTC TGGAACTCAA GTAGAGAGTG ACTCCTTCCA GTTCCAGGTG
    TCCAGCTCTG CCAGTCCACA AGCCAGCTTG GAAAGCCACG TGTTCAACGT TGCTGTGCTC
    CCACAGACAC CCAGAGCACC TCAGCTTTCC CTTGGCTCCT CTTTGCACAT GGCTGTGCTG
    GAGGACCGCG TGACAGTGAT TGAGCCCCAT CACCTCTCCT TTGTAGACCC AGAGATTCCC
    AGTGAGAAAA TCCTGTTCAA CGTGACTGTC CCTCTGCTTC CTGGCCAAGG TATTGTGGAG
    CACAGAGACA GGCCTCACTC GCCAGTCAGG TATTTCACTC AAGCAGATAT AAACCATGGC
    AAGATTGCTT ATCGTCCACC AACTGCAGCT CCACACTTGA GAGAGATCAT TGCATTCTCA
    TTTGCAGGTC TCCCAGAGTC AGTGAACTTC CGATTCACAG TGTCTGGTGG AGAACACACA
    ACCCCGGAGA TGGCGTTTGT CATTCACTTG CTCTCTGCAG AGCAGCAGCC TCCAGTCTTC
    CAGATCACAG CTCCTTTCCT GGAGGTCAGC CAGGGGGGAA AGGCCACCAT CGGTGTCCAG
    TTGGCTGTGA GTGACACAGA TACAGCTCCT GAGGGCCTCT TCTTTGAGCT GGTGAAACCT
    CCCCATCATG GCATCGTGCT CAAGCATGAT GCAGGTGTGC AGGAGCACAT GAGAGCAGGT
    GACACCTTCA CCTATGAGGA CGTCACTCGA AATGCTCTGC AGTACGTACA TGATGGCTCA
    TCGGCAGCAG AAGACAGCAT GGAAATCTCT GTCACCGATG GCGTCACCAC AGTGACCACA
    GTGCTGAGAG TGGAGGTGTC CCTACTGGAT AACAACGGCC CACAGCTGGC CCCAGGCTGC
    CTGTTGGCAA TCACTGTGGC TAGTAAAAGC TCTGCAACTC TCTCTCGACT GCACCTCGCT
    TACATTGACA ACAATTCTCC TGACTCAGAG ATAAGAATCC AGTTAATATC TCTGCCCACG
    TACGGCACCC TCTTACGGAC GTCTGGCTCT CATGCTGAAG AACTTTCTAG GGTTTATAAT
    TTCACCATGG AAGACATCAA CAACCAGAGG ATCAGTTACT CCACTGTGTT TGAGACAAGC
    AATCAGCCAG TTACGGATGC CTTTCACTTC ACTGTTTTTG ATGCTGATAA TAACCGGCTT
    GACAGACAGA TGTTTACCAT CACCATCACG TCTGCCCAGA CAGCACCATC ACTCATTGTG
    TTTGCTGACC ATATCGCTGT GGATGAGGGG GGCAGGGTCC CACTTCTGGC TCATCACCAC
    TTAACTGCTG ATTATGATAC TGCTGCCAAG GAAGACCTGA GGGTCACGGT TATCACTCTA
    CCCGTGTATG GCTACATTGA AAACACTAAG ACAGGTGAGA CTTTTGGACC ACAGAGTGCT
    GTGACCACAG ATGCATCCTT CTCCATTCAA GATGTTCTAG AGAACAACAT TTACTACTTT
    CAGAGCGTCC ATGAAAGCAT CGAGCCAACA AGTGATGTTT TCAGCTTTTA CGTGAGCGAT
    GGCTTCAGCC AGTCTGAGGT GCACAGCATC AACATCACCA TTCAGCGACG GAATGATGAG
    CCCCCAAAGA TGGTGTTGGA GCCTGTCAGA GTGCATGAGA GCTCAGTGGT TGTTACCAGT
    GCCTCACTCA ACCTGCAGGA CCTGGACACC CAGAACAGTG AGCTTGTCAT CATGGTTACC
    AAAACTCCTG AGCACGGTCA CCTTCGCAGG AGGCAGACTG CTGTGGAGCC CCCAGAGCGT
    GGACACGTGC TGTCTCTGGG CTCATCCTTC ACCTACCAGG ACATTTTGGA TGAGCTGATT
    GTTTATTCTC AAAGCGGTGC AGCAGCACAG ACGGATGAGT TTGGCTTCTC CATCACAGAT
    GGTCTCTACA TGCAGACAGG GAAGCTGGAG TTCTCCATGG AGCTTCCAAA GAAGCAGCTG
    CCCACATTGA CTGCCAGCAG GGTCCTGCAG CTCCCCACTG GCTCTGCAGC ACACATCACA
    GATCAACACT TGAAAGCAGC AGCTGTTTAT CCAGATGACA CAATGATCAG ATTTGTCATG
    AAGAGGGATC CCAGCGTGGG CCATTTGCAG TTGACTGATA TTGATAATCC AGTCGAGATC
    TCTGTCAGAG GGCCTGTCAA AAGTTTTACC CAGGCTGATA TAAATAAAGG GCAAGTGGTT
    TATAGTCACA AGAAGGGGGA TCCTGGTGGA AATTTTGCCT TCACCTTTGA TGTGATCGAT
    GCAGATGGCA GCAGACTGAT GGACCAGTTT TTTCATATCA CTGTATTAGA AGACAGGTTC
    CCCCCCTCCA TCGTCGCTAA CACAGGGCTG GTCTTAGATG AGAACTCTGC AAAGAAGATC
    ACAACACTGC AGCTCTCTGC CACTGACCGG GACAGTGAGC CGACCCAGCT CCAGTACAAG
    CTCACCAAGC AGCCACAGCT GGGGCACCTG GAGCATGTGG CCTCCCCAGG GACAAAAATC
    AGTTCTTTCA GCCAGGCAGA CTTGGCCTCT CGCAGCATCC AATACGTTCA CACTAGCGAC
    ACTGAAAAGC ATACGGATGC CTTCACCTTC TCTGTGTCTG ATGGAACAAA TGAGGTCAGC
    CAGACATTTG ACATCACCAT AAACCCTGTG GATGATTCCT TACCTGTAGT GCAAAGCTTT
    GGGATGAGAG TTCAGGAAGG TGTGAGAAAA ACCATCACAG AGTTTGAGCT TAAAGCAACA
    GATGCTGACA CAAAGGCTGA GGCAATTACA TTCACAGTCG TGCAGCCCCC TCGACATGGC
    CTGATTGAAC ACACCAGCAA TGGGCAGCAT TACCACCAAG CCACCACCTT CACCATGGAT
    GACATTTACC AGAACCGCAT CAGCTACAGT CACGATGGAA GCAACTCACT GAAGGATCGC
    TTTGCTTTCA CTGTGTCTGA TGGGACCAAT CCCTTCTTCA TTATGGAGGA TGGGGGGAAA
    AAGGTTGTGA CAGCAGCACC ACAGCGCTTC AGAATGGACA TCCTCCCTGT GGATGATGGG
    ACACCCAGGG TGGTCACCAA CCAGGGTTTG CAATGGCTGG AGTATGTGGA TGGCAAGGCA
    ACCAATCTAA TCACCAAGAA GGAATTACTG ACAACAGATC CTGACACAGA TGACAAACAG
    CTGATCTATG AGATAACAGA TGGTCCCAGG AATGGTTATG TGGAGAACAA GCTGAAACCA
    GGAACAACTG TGACCATGTT TTCTCAGGAG GATGTGAATC AGGGCCTCAT TCGGTATGTG
    ATGCATGAGG AGAAGGTTCA GGAGACTATG GACAGTTTTC AGTTCCTCGT GAAGGACAGC
    AAACCCAATG TGGTCCGTGG CAACGTCTTC CACATCCAGT GGTCTCTCCT CAGCTTTACA
    TACAGCAGCT ACAACGTCAG TGAGAGCGCT GGCTCTGTGA GCGTCACCGT GCGGCGGATT
    GGTAACCTTA ACCAGTATGC CATTGTCTTG TGCCGCACGG AGCAGGGAAC AGCCACTTCC
    AGCTCTGGTT CACAGTCTGA AGAGCAGGAC TATGTGGAGT ACGCAGGGCA GGTGCAGTTT
    GAGGAGCGGG AAGACACCAA GGCCTGCACC ATCGTCATTA ACGATGACGA CATCTTTGAG
    AGCACCGAGA GCTTCACTGT GGAGCTCAGC ATGCCAGCCT ATGCTCTGCT GGGGAATGTC
    ACCCATGCCA CAGTCACCAT CACAGACGCT GAGGATGAGC CCATGCTGCA GTTCGACAGG
    AAGACGTACC ACGTCAGTGA GAGCGCTGGG TTTCTCTCTG CGCCTGTTGA AAGGAAAGGA
    GATGCTAGCA GCACTGTGTC TGCTGTCTGC TACACTGTCC CCAAATCAGC CAAGGGAAGC
    AGCCTTTATA TGCTGGAGTC TGGCTCCGAC TTCAAGTCCC GAGGGATGAC AGATGACAGC
    CGCATAGTTT TTGGACCAGG TGTTACCATG TCGACATGTG ATGTCATGCT TATTGATGAC
    AGTGAATATG AAGAAGAAGA GGAGTTTGAG ATAGTATTGG CTGATGCTTC GGAAAATGCA
    CGCATTGGCA ACCGGGCTTC AGCCAGCGTC TTCATCGGTG GTCCCAATGA TGCCTCGACA
    GTGTTCCTGG GAAATGCCAC GTTCACCGTC AGTGAGGCTG CAGGAAACAT TGAAATTCCA
    GTCCTCCGTC ACGGACCTGA TCTTTCAACC CCCACCTCAG TGTGGTGTGC TACTCGGACA
    TCAGATCCTC CATCTGCCAG CCCAGGAGTT GATTATGTCC CCAGCTCCAG AAAAATAGAG
    TTCAGGCCAG GCAAGACAGA AGAGTACCAA CCATGCAGTT CACCAAGGAT GTGTTCACAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.