AFF2 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	AFF2
Entrez Gene ID	422394
Full Name	AF4/FMR2 family member 2
General protein information	Preferred Names AF4/FMR2 family member 2 Names AF4/FMR2 family member 2
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015278653.1	XP_015134139.1	AF4/FMR2 family member 2
XM_025150465.1	XP_025006233.1	AF4/FMR2 family member 2 isoform X3
XM_025150467.1	XP_025006235.1	AF4/FMR2 family member 2 isoform X5
XM_025150468.1	XP_025006236.1	AF4/FMR2 family member 2 isoform X6
XM_025150464.1	XP_025006232.1	AF4/FMR2 family member 2 isoform X2
XM_025150466.1	XP_025006234.1	AF4/FMR2 family member 2 isoform X4
XM_015278653.2	XP_015134139.2	AF4/FMR2 family member 2 isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	aff2	XP_002934593.2
Homo sapiens (human)	AFF2	NP_001162593.1
Gallus gallus (chicken)	AFF2	XP_003641137.2
Danio rerio (zebrafish)	LOC100330019	XP_002664429.2
	AFF2	XP_001088220.2

Gene Ontology

The following AFF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa34892	XM_015278653.1 Latest version!	Gallus gallus AF4/FMR2 family, member 2 (AFF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OGa150688	XM_015278653.2 Latest version!	Gallus gallus AF4/FMR2 family member 2 (AFF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa150689	XM_025150464.1 Latest version!	Gallus gallus AF4/FMR2 family member 2 (AFF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa150690	XM_025150465.1 Latest version!	Gallus gallus AF4/FMR2 family member 2 (AFF2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa150691	XM_025150466.1 Latest version!	Gallus gallus AF4/FMR2 family member 2 (AFF2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa150692	XM_025150467.1 Latest version!	Gallus gallus AF4/FMR2 family member 2 (AFF2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa150693	XM_025150468.1 Latest version!	Gallus gallus AF4/FMR2 family member 2 (AFF2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AFF2 ( XM_015278653.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134139.1 Gallus gallus AF4/FMR2 family, member 2 (AFF2), mRNA.

Price & Availability↑

CloneID	OGa34892
Clone ID Related Accession (Same CDS sequence)	XM_015278653.1
Accession Version	XM_015278653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	AF4/FMR2 family member 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763735.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGTTG TGCATTGGAT CAGCAACAGC GTAAGTGGAT CGAGGAGTGC AGCAAGCTGT 
GGATCGAAGG TTACCCTTCC CAATGACTCC AGCAGTGTGG AAGAAATACT GCGTGAGATG 
TCCCATTCCT GGCCTCCTCC TCTCACTGCT ATTCACACTC CTGGAAAGGC TGAACAGACC 
AAGTTTTCTA TCCCAAGCAA AGTACAAGAC TCCCAACATC TGACCTCAGG ATACAATGTA 
CAAAAGTGGA GTGATCCAGG AGGGAAAGTT GCAACTAAGT CAGTGACACA AAAGTCGATG 
CTTGAGGATG ATCTGAAACT CAGCAGTGAT GAAGATGACA ATGAACAGGC TGCTGAAAAG 
ACAAAATTGA GAAACATTCC TGTAAACGGC CTGCCGGTGT CGGTGGCACA TGCAGCTGGC 
TTAGCCCATT CCAGTGGTGG CTCTGGGAGC TCCAGTGAGT CCGAGAGCAG CTCTGAGTCT 
GACTCCGACA GTGAGAGCAG CTCCAGTGAC AGCGACTGCA ATGAGGAATC ACGCAGTGCC 
ACGCCAGAGC CTGAACCTCC CTCAACAAAC AAATGGCAGC TGGATAAGTG GCTAAATAAA 
GTGAACCCCC ACAACAAGAC CATCATCAGC AATCAAAATG AGCCTCACAG TGAGACCAGC 
TCCCAGCCAC AGAACAGCCA GCAGGCTGAA GGGCAAAACA AAGGGAAGCT CAGCAACAGC 
CTGCCCCCAG CTGATTCCAA AGACCAGGCG ATCCTCAGTC CCATCCGAGA GAAAGCCAAA 
CCACGGGTTG CCCAGAAAAC TCCAGAGGCA AAAAGCTCCA AGCAGAAGTC GCCAGCTCAT 
AATGAGCCAA CTGCACAAAG GACTACTGGG AAAAAGCAAC CCAAAAAGGT AGAAAGAACT 
TCCAGTGTAG AAGAGTATAA CTGGCCCAAA CCAAATAATA CCAGCAACAC TCCCAAAGAA 
AAAGACACAC AGGAACCCCC CGAGCAGCCA AAGGTTCGAA CTAAAGCAGC AGTTGGCAAG 
ACTGCCCCAA GGAAGGAACC TCGAACAAGC ACAGGTCTCA CTTCGGAGAA GAAAAAGTAC 
AGAGGCCCCA GCAAAATTGT CCCCAAGTCC CGAGAATTTA TTGAAACTGA TTCATCTACT 
TCTGACTCAA ATACAGATCA GGAGGAGAGC CTGCAGGCTA AGATATTGCC AGCTTGTAGT 
GGACCAGCCA ACAGCATCAA AGTGAAGGAC CCTGGCAGTA ACATCCTCAG TGTAAGTAGC 
TTAACTAGCA ATGGCAGCAA CACATCCATC GACCAGACTA GCAATGACTT GGAGGAGCAG 
CTCTTTTCTC CTATCCCAAT TGTACAAAAT GAACTTCTGT CCCCACTGAG AGAATATGAA 
GAACTCAAAT CTCTTTGGGT GAAAATAGAC CTTAGTTTAC TCTCTAGAAT ACCTGGACAG 
GAGCCTTTTG AGACCATATC TGTGAAAACT GAACCAAGAG AGGCAAATGT GAAACACAGA 
CGACCATCTC GAGAGTCCCC TACCCCAGCA GCTGAAAAAA CATCTGCAAA ATCCAAGAGG 
AAACACAAGT CGGAAAACCT GGAAACCTTT GTTGAAACCA AGAAGCAGCG CCTGGAGGAC 
CCTTCAGCTT CGTGCTTGCT CCCACCTTGT ATCTCTCCAA TACCAAACCA CAGATTAACC 
AGCACTAAAG AGAGCAGCAG TAACTTGGTG AAGAAGATGG CAAAGAAAAG AGAAGAGAAG 
CTCTTCCCTC CTCCATTATC CCCCCTGTTG GATGAGCCTG TGCACCGTCG GCACAGCAAT 
GACAACAGCT CCTTCAGCCA AGAAACCAGT ATCACAAACA CCTGTCAGAC CAGCAGCTCT 
TCCTCCTCAG TTTCTAAACA CAGAAAGAAT GAAAATAAAT CTAACCCCAA AGGAATCTGT 
GAGGAAGAAA CCCACAATGC ACCAACAGCC AATGCTCTTC TTGATACGAA CCATCTAGAA 
ACAGATATCT GGTCTGCAAT GCCAACTCTT TCCAACGGGA ATTCTGAGCC CAGAAGACCT 
AAAATCACAT TTGATGATGT GCTTCACAAT GCAGATTATT ATATGCAAGA AGCGAAGAAG 
CTAAAGCATA AAGCGGATGC ATTGCTGGAG AAGTTCGGCA AAGCAGTGAA TTATGCTGAT 
GCTGCCTTGT CCTTCATCGA GTGTGGGAAT GCTATGGAGC GAGATCCATT AGAAGCTAAA 
TCTCCATACA CCATGTACTC AGAGACTGTG GAACTCATCA GGTATGCAAT GAGACTCAAG 
AATTTCACAG GCTCTTCTGC CACAGAAGGG GACAAGAAAC TAGCAGTTTT ATGCTACCGA 
TGCTTATCGC TTCTGTACTT GAGAATGTTT AAACTAAAGA AGGACCATGC TATGAAGTAC 
TCCAGATCTC TGATGGAATA TTTTAAGAAC TCTTCAAAAG CCTCACAGGC CCCCTCACCT 
TGGGGCGGCA ATGGAAAGAG CACAGAAACA CCATCACCCA TGCCCCTGAG CCCATCCCCT 
GTCAGCTCTG CTGGAGGCAG TGCAGGGATC ACTGGCTCCT CCTCAGCAGG GACCATCACC 
ATTCCTCAGC GCATTCACCA CATGGCTGCC AGCCATGTCA ACATCACCAA CAATGTTCTG 
CGAAGCTACG AGCACTGGGA CATGGCTGAC AAGCTGACTA AGGAGAATAA AGAATTCTTT 
GTGGACCTGG ACACAGTGAT GGGACCCTTA ACACAACACA GCAGTATGAC CAATCTGGTT 
CGTTACGTTC GCCAAGGACT CCACTGGCTC CACATTGAGG CTCATTTGTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134139.1
CDS	3..2816
Translation	MGVVHWISNSVSGSRSAASCGSKVTLPNDSSSVEEILREMSHSWPPPLTAIHTPGKAEQTKFSIPSKVQDSQHLTSGYNVQKWSDPGGKVATKSVTQKSMLEDDLKLSSDEDDNEQAAEKTKLRNIPVNGLPVSVAHAAGLAHSSGGSGSSSESESSSESDSDSESSSSDSDCNEESRSATPEPEPPSTNKWQLDKWLNKVNPHNKTIISNQNEPHSETSSQPQNSQQAEGQNKGKLSNSLPPADSKDQAILSPIREKAKPRVAQKTPEAKSSKQKSPAHNEPTAQRTTGKKQPKKVERTSSVEEYNWPKPNNTSNTPKEKDTQEPPEQPKVRTKAAVGKTAPRKEPRTSTGLTSEKKKYRGPSKIVPKSREFIETDSSTSDSNTDQEESLQAKILPACSGPANSIKVKDPGSNILSVSSLTSNGSNTSIDQTSNDLEEQLFSPIPIVQNELLSPLREYEELKSLWVKIDLSLLSRIPGQEPFETISVKTEPREANVKHRRPSRESPTPAAEKTSAKSKRKHKSENLETFVETKKQRLEDPSASCLLPPCISPIPNHRLTSTKESSSNLVKKMAKKREEKLFPPPLSPLLDEPVHRRHSNDNSSFSQETSITNTCQTSSSSSSVSKHRKNENKSNPKGICEEETHNAPTANALLDTNHLETDIWSAMPTLSNGNSEPRRPKITFDDVLHNADYYMQEAKKLKHKADALLEKFGKAVNYADAALSFIECGNAMERDPLEAKSPYTMYSETVELIRYAMRLKNFTGSSATEGDKKLAVLCYRCLSLLYLRMFKLKKDHAMKYSRSLMEYFKNSSKASQAPSPWGGNGKSTETPSPMPLSPSPVSSAGGSAGITGSSSAGTITIPQRIHHMAASHVNITNNVLRSYEHWDMADKLTKENKEFFVDLDTVMGPLTQHSSMTNLVRYVRQGLHWLHIEAHLL

Target ORF information:

RefSeq Version	XM_015278653.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus AF4/FMR2 family, member 2 (AFF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015278653.1

ORF Insert Sequence:

ATGGGAGTTG TGCATTGGAT CAGCAACAGC GTAAGTGGAT CGAGGAGTGC AGCAAGCTGT 
GGATCGAAGG TTACCCTTCC CAATGACTCC AGCAGTGTGG AAGAAATACT GCGTGAGATG 
TCCCATTCCT GGCCTCCTCC TCTCACTGCT ATTCACACTC CTGGAAAGGC TGAACAGACC 
AAGTTTTCTA TCCCAAGCAA AGTACAAGAC TCCCAACATC TGACCTCAGG ATACAATGTA 
CAAAAGTGGA GTGATCCAGG AGGGAAAGTT GCAACTAAGT CAGTGACACA AAAGTCGATG 
CTTGAGGATG ATCTGAAACT CAGCAGTGAT GAAGATGACA ATGAACAGGC TGCTGAAAAG 
ACAAAATTGA GAAACATTCC TGTAAACGGC CTGCCGGTGT CGGTGGCACA TGCAGCTGGC 
TTAGCCCATT CCAGTGGTGG CTCTGGGAGC TCCAGTGAGT CCGAGAGCAG CTCTGAGTCT 
GACTCCGACA GTGAGAGCAG CTCCAGTGAC AGCGACTGCA ATGAGGAATC ACGCAGTGCC 
ACGCCAGAGC CTGAACCTCC CTCAACAAAC AAATGGCAGC TGGATAAGTG GCTAAATAAA 
GTGAACCCCC ACAACAAGAC CATCATCAGC AATCAAAATG AGCCTCACAG TGAGACCAGC 
TCCCAGCCAC AGAACAGCCA GCAGGCTGAA GGGCAAAACA AAGGGAAGCT CAGCAACAGC 
CTGCCCCCAG CTGATTCCAA AGACCAGGCG ATCCTCAGTC CCATCCGAGA GAAAGCCAAA 
CCACGGGTTG CCCAGAAAAC TCCAGAGGCA AAAAGCTCCA AGCAGAAGTC GCCAGCTCAT 
AATGAGCCAA CTGCACAAAG GACTACTGGG AAAAAGCAAC CCAAAAAGGT AGAAAGAACT 
TCCAGTGTAG AAGAGTATAA CTGGCCCAAA CCAAATAATA CCAGCAACAC TCCCAAAGAA 
AAAGACACAC AGGAACCCCC CGAGCAGCCA AAGGTTCGAA CTAAAGCAGC AGTTGGCAAG 
ACTGCCCCAA GGAAGGAACC TCGAACAAGC ACAGGTCTCA CTTCGGAGAA GAAAAAGTAC 
AGAGGCCCCA GCAAAATTGT CCCCAAGTCC CGAGAATTTA TTGAAACTGA TTCATCTACT 
TCTGACTCAA ATACAGATCA GGAGGAGAGC CTGCAGGCTA AGATATTGCC AGCTTGTAGT 
GGACCAGCCA ACAGCATCAA AGTGAAGGAC CCTGGCAGTA ACATCCTCAG TGTAAGTAGC 
TTAACTAGCA ATGGCAGCAA CACATCCATC GACCAGACTA GCAATGACTT GGAGGAGCAG 
CTCTTTTCTC CTATCCCAAT TGTACAAAAT GAACTTCTGT CCCCACTGAG AGAATATGAA 
GAACTCAAAT CTCTTTGGGT GAAAATAGAC CTTAGTTTAC TCTCTAGAAT ACCTGGACAG 
GAGCCTTTTG AGACCATATC TGTGAAAACT GAACCAAGAG AGGCAAATGT GAAACACAGA 
CGACCATCTC GAGAGTCCCC TACCCCAGCA GCTGAAAAAA CATCTGCAAA ATCCAAGAGG 
AAACACAAGT CGGAAAACCT GGAAACCTTT GTTGAAACCA AGAAGCAGCG CCTGGAGGAC 
CCTTCAGCTT CGTGCTTGCT CCCACCTTGT ATCTCTCCAA TACCAAACCA CAGATTAACC 
AGCACTAAAG AGAGCAGCAG TAACTTGGTG AAGAAGATGG CAAAGAAAAG AGAAGAGAAG 
CTCTTCCCTC CTCCATTATC CCCCCTGTTG GATGAGCCTG TGCACCGTCG GCACAGCAAT 
GACAACAGCT CCTTCAGCCA AGAAACCAGT ATCACAAACA CCTGTCAGAC CAGCAGCTCT 
TCCTCCTCAG TTTCTAAACA CAGAAAGAAT GAAAATAAAT CTAACCCCAA AGGAATCTGT 
GAGGAAGAAA CCCACAATGC ACCAACAGCC AATGCTCTTC TTGATACGAA CCATCTAGAA 
ACAGATATCT GGTCTGCAAT GCCAACTCTT TCCAACGGGA ATTCTGAGCC CAGAAGACCT 
AAAATCACAT TTGATGATGT GCTTCACAAT GCAGATTATT ATATGCAAGA AGCGAAGAAG 
CTAAAGCATA AAGCGGATGC ATTGCTGGAG AAGTTCGGCA AAGCAGTGAA TTATGCTGAT 
GCTGCCTTGT CCTTCATCGA GTGTGGGAAT GCTATGGAGC GAGATCCATT AGAAGCTAAA 
TCTCCATACA CCATGTACTC AGAGACTGTG GAACTCATCA GGTATGCAAT GAGACTCAAG 
AATTTCACAG GCTCTTCTGC CACAGAAGGG GACAAGAAAC TAGCAGTTTT ATGCTACCGA 
TGCTTATCGC TTCTGTACTT GAGAATGTTT AAACTAAAGA AGGACCATGC TATGAAGTAC 
TCCAGATCTC TGATGGAATA TTTTAAGAAC TCTTCAAAAG CCTCACAGGC CCCCTCACCT 
TGGGGCGGCA ATGGAAAGAG CACAGAAACA CCATCACCCA TGCCCCTGAG CCCATCCCCT 
GTCAGCTCTG CTGGAGGCAG TGCAGGGATC ACTGGCTCCT CCTCAGCAGG GACCATCACC 
ATTCCTCAGC GCATTCACCA CATGGCTGCC AGCCATGTCA ACATCACCAA CAATGTTCTG 
CGAAGCTACG AGCACTGGGA CATGGCTGAC AAGCTGACTA AGGAGAATAA AGAATTCTTT 
GTGGACCTGG ACACAGTGAT GGGACCCTTA ACACAACACA GCAGTATGAC CAATCTGGTT 
CGTTACGTTC GCCAAGGACT CCACTGGCTC CACATTGAGG CTCATTTGTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF2 ( XM_015278653.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134139.2 Gallus gallus AF4/FMR2 family member 2 (AFF2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa150688
Clone ID Related Accession (Same CDS sequence)	XM_015278653.2
Accession Version	XM_015278653.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	AF4/FMR2 family member 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015278653.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTATGT GTGCGCGCGC CAGGGCCGCT GCCGCCGCTG TGCCCGCCTC TCCCAGCCAG 
GCAGCAGCCG CGCGCGCTGC TGGCGGCGGG CGGCGGCGGC GGCGCACCAC GAGCCCCGGG 
CGGCTCCGCG GCGGCCGCCG CGGCCGCCGC TGCCCGTGCC CCTGCCCCTG CCGCGCTGCC 
TCTGCTCGGC TCGGCGCGGG GAGAGGGTAC CACTTCTTCG AGTCTCCGCA CCAGATCGTA 
ATGGACCTAT TCGACTTTTT CAGGGACTGG GACTTAGAGC AGCAGTGTCA CTATGAGCAA 
GACCGGAATG CACTTAAGCG AAAGGAATGG GAGAGAAGAA ACCAAGAAGT CCAGCAAGAT 
GAAGACCTCT TTGCTTCAGG TTTTAATCTC TTTGGGGAAC CCTACAAGAC AAATAAAGGT 
GATGCTCTTG CAAACCGTGT CCAGAACACA TTGGGAAACT ATGATGAGAT GAAGGATCTG 
CTAACTAACC ATTCCAATCA GAGCCATCTT GTAGGAATCC CAAAGAATTC TGTCCCACAG 
ACACCCATCG AGAAAAATGA ACAGAACTTT TTCTCAGAAC AAAGAAACAG GGTGATCCCA 
TCTCATCAGA TCAGTGGCCA CTCATCGACA TCAATGCCTC CACCTTCGTC ACTGTCATCT 
AATTCTGCTT TGCTGCACAG TCACCAGAGC AGCAGGAAGT CGAGAACAGA CTGGTCACGC 
AGTGGTCACA ACTGTAGTGG GACCCAGCAA AGCCAATCCA GTAGTCAGCA AAGTCGGACA 
AAGCATAGCT CTTCTCATGA CCTGCCACAA GGCAGATATG AAGACCTCTA TAATTGTCAG 
AGTGAGCAGC ATAAAGGTGG AGGAACTGAG GAAGCAAATT CTATGGCAGC ATCTTCACAT 
TCAAGGCGGC ATACTCATTC CAAGTCAGCA GCTGGAGAAC ATACTTACAA AGAAAACAGT 
CATTCAAAGT CACCCACGGA GCTTGAGTTT GTAGGTCATG GTCCTGGATC TCCACTTCCT 
TCCACATCTT TGTTGTCTTC AAACAATGGT CTTTCAAGCC AGAATTTCCC ACCAGGACTT 
CACTGTAAAA GCAGCATGGT CCAGCAAAAG CCTACAGCAT ATGTTAGGCC GATGGATGGT 
CAGGACCAGG TACCGAATGA CTCACCTGAA CTGAAACCTC CCATAGAAAT TGAGAGTGGA 
TATGGAAATC AGTCCTTTGG AACACTGTTG GAAGGAAAAG TGAACACACC TAGTTCAAAA 
AATAAAGTAC CAAAGCTTAA TATTCCTCCT GTTGGTGAAG CAATGGGAGT TGTGCATTGG 
ATCAGCAACA GCGTAAGTGG ATCGAGGAGT GCAGCAAGCT GTGGATCGAA GGTTACCCTT 
CCCAATGACT CCAGCAGTGT GGAAGAAATA CTGCGTGAGA TGTCCCATTC CTGGCCTCCT 
CCTCTCACTG CTATTCACAC TCCTGGAAAG GCTGAACAGA CCAAGTTTTC TATCCCAAGC 
AAAGTACAAG ACTCCCAACA TCTGACCTCA GGATACAATG TACAAAAGTG GAGTGATCCA 
GGAGGGAAAG TTGCAACTAA GTCAGTGACA CAAAAGTCGA TGCTTGAGGA TGATCTGAAA 
CTCAGCAGTG ATGAAGATGA CAATGAACAG GCTGCTGAAA AGACAAAATT GAGAAACATT 
CCTGTAAACG GCCTGCCGGT GTCGGTGGCA CATGCAGCTG GCTTAGCCCA TTCCAGTGGT 
GGCTCTGGGA GCTCCAGTGA GTCCGAGAGC AGCTCTGAGT CTGACTCCGA CAGTGAGAGC 
AGCTCCAGTG ACAGCGACTG CAATGAGGAA TCACGCAGTG CCACGCCAGA GCCTGAACCT 
CCCTCAACAA ACAAATGGCA GCTGGATAAG TGGCTAAATA AAGTGAACCC CCACAACAAG 
ACCATCATCA GCAATCAAAA TGAGCCTCAC AGTGAGACCA GCTCCCAGCC ACAGAACAGC 
CAGCAGGCTG AAGGGCAAAA CAAAGGGAAG CTCAGCAACA GCCTGCCCCC AGCTGATTCC 
AAAGACCAGG CGATCCTCAG TCCCATCCGA GAGAAAGCCA AACCACGGGT TGCCCAGAAA 
ACTCCAGAGG CAAAAAGCTC CAAGCAGAAG TCGCCAGCTC ATAATGAGCC AACTGCACAA 
AGGACTACTG GGAAAAAGCA ACCCAAAAAG GTAGAAAGAA CTTCCAGTGT AGAAGAGTAT 
AACTGGCCCA AACCAAATAA TACCAGCAAC ACTCCCAAAG AAAAAGACAC ACAGGAACCC 
CCCGAGCAGC CAAAGGTTCG AACTAAAGCA GCAGTTGGCA AGACTGCCCC AAGGAAGGAA 
CCTCGAACAA GCACAGGTCT CACTTCGGAG AAGAAAAAGT ACAGAGGCCC CAGCAAAATT 
GTCCCCAAGT CCCGAGAATT TATTGAAACT GATTCATCTA CTTCTGACTC AAATACAGAT 
CAGGAGGAGA GCCTGCAGGC TAAGATATTG CCAGCTTGTA GTGGACCAGC CAACAGCATC 
AAAGTGAAGG ACCCTGGCAG TAACATCCTC AGTGTAAGTA GCTTAACTAG CAATGGCAGC 
AACACATCCA TCGACCAGAC TAGCAATGAC TTGGAGGAGC AGCTCTTTTC TCCTATCCCA 
ATTGTACAAA ATGAACTTCT GTCCCCACTG AGAGAATATG AAGAACTCAA ATCTCTTTGG 
GTGAAAATAG ACCTTAGTTT ACTCTCTAGA ATACCTGGAC AGGAGCCTTT TGAGACCATA 
TCTGTGAAAA CTGAACCAAG AGAGGCAAAT GTGAAACACA GACGACCATC TCGAGAGTCC 
CCTACCCCAG CAGCTGAAAA AACATCTGCA AAATCCAAGA GGAAACACAA GTCGGAAAAC 
CTGGAAACCT TTGTTGAAAC CAAGAAGCAG CGCCTGGAGG ACCCTTCAGC TTCGTGCTTG 
CTCCCACCTT GTATCTCTCC AATACCAAAC CACAGATTAA CCAGCACTAA AGAGAGCAGC 
AGTAACTTGG TGAAGAAGAT GGCAAAGAAA AGAGAAGAGA AGCTCTTCCC TCCTCCATTA 
TCCCCCCTGT TGGATGAGCC TGTGCACCGT CGGCACAGCA ATGACAACAG CTCCTTCAGC 
CAAGAAACCA GTATCACAAA CACCTGTCAG ACCAGCAGCT CTTCCTCCTC AGTTTCTAAA 
CACAGAAAGA ATGAAAATAA ATCTAACCCC AAAGGAATCT GTGAGGAAGA AACCCACAAT 
GCACCAACAG CCAATGCTCT TCTTGATACG AACCATCTAG AAACAGATAT CTGGTCTGCA 
ATGCCAACTC TTTCCAACGG GAATTCTGAG CCCAGAAGAC CTAAAATCAC ATTTGATGAT 
GTGCTTCACA ATGCAGATTA TTATATGCAA GAAGCGAAGA AGCTAAAGCA TAAAGCGGAT 
GCATTGCTGG AGAAGTTCGG CAAAGCAGTG AATTATGCTG ATGCTGCCTT GTCCTTCATC 
GAGTGTGGGA ATGCTATGGA GCGAGATCCA TTAGAAGCTA AATCTCCATA CACCATGTAC 
TCAGAGACTG TGGAACTCAT CAGGTATGCA ATGAGACTCA AGAATTTCAC AGGCTCTTCT 
GCCACAGAAG GGGACAAGAA ACTAGCAGTT TTATGCTACC GATGCTTATC GCTTCTGTAC 
TTGAGAATGT TTAAACTAAA GAAGGACCAT GCTATGAAGT ACTCCAGATC TCTGATGGAA 
TATTTTAAGA ACTCTTCAAA AGCCTCACAG GCCCCCTCAC CTTGGGGCGG CAATGGAAAG 
AGCACAGAAA CACCATCACC CATGCCCCTG AGCCCATCCC CTGTCAGCTC TGCTGGAGGC 
AGTGCAGGGA TCACTGGCTC CTCCTCAGCA GGGACCATCA CCATTCCTCA GCGCATTCAC 
CACATGGCTG CCAGCCATGT CAACATCACC AACAATGTTC TGCGAAGCTA CGAGCACTGG 
GACATGGCTG ACAAGCTGAC TAAGGAGAAT AAAGAATTCT TTGTGGACCT GGACACAGTG 
ATGGGACCCT TAACACAACA CAGCAGTATG ACCAATCTGG TTCGTTACGT TCGCCAAGGA 
CTCCACTGGC TCCACATTGA GGCTCATTTG TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134139.2
CDS	16..4131
Translation	MCMCARARAAAAAVPASPSQAAAARAAGGGRRRRRTTSPGRLRGGRRGRRCPCPCPCRAASARLGAGRGYHFFESPHQIVMDLFDFFRDWDLEQQCHYEQDRNALKRKEWERRNQEVQQDEDLFASGFNLFGEPYKTNKGDALANRVQNTLGNYDEMKDLLTNHSNQSHLVGIPKNSVPQTPIEKNEQNFFSEQRNRVIPSHQISGHSSTSMPPPSSLSSNSALLHSHQSSRKSRTDWSRSGHNCSGTQQSQSSSQQSRTKHSSSHDLPQGRYEDLYNCQSEQHKGGGTEEANSMAASSHSRRHTHSKSAAGEHTYKENSHSKSPTELEFVGHGPGSPLPSTSLLSSNNGLSSQNFPPGLHCKSSMVQQKPTAYVRPMDGQDQVPNDSPELKPPIEIESGYGNQSFGTLLEGKVNTPSSKNKVPKLNIPPVGEAMGVVHWISNSVSGSRSAASCGSKVTLPNDSSSVEEILREMSHSWPPPLTAIHTPGKAEQTKFSIPSKVQDSQHLTSGYNVQKWSDPGGKVATKSVTQKSMLEDDLKLSSDEDDNEQAAEKTKLRNIPVNGLPVSVAHAAGLAHSSGGSGSSSESESSSESDSDSESSSSDSDCNEESRSATPEPEPPSTNKWQLDKWLNKVNPHNKTIISNQNEPHSETSSQPQNSQQAEGQNKGKLSNSLPPADSKDQAILSPIREKAKPRVAQKTPEAKSSKQKSPAHNEPTAQRTTGKKQPKKVERTSSVEEYNWPKPNNTSNTPKEKDTQEPPEQPKVRTKAAVGKTAPRKEPRTSTGLTSEKKKYRGPSKIVPKSREFIETDSSTSDSNTDQEESLQAKILPACSGPANSIKVKDPGSNILSVSSLTSNGSNTSIDQTSNDLEEQLFSPIPIVQNELLSPLREYEELKSLWVKIDLSLLSRIPGQEPFETISVKTEPREANVKHRRPSRESPTPAAEKTSAKSKRKHKSENLETFVETKKQRLEDPSASCLLPPCISPIPNHRLTSTKESSSNLVKKMAKKREEKLFPPPLSPLLDEPVHRRHSNDNSSFSQETSITNTCQTSSSSSSVSKHRKNENKSNPKGICEEETHNAPTANALLDTNHLETDIWSAMPTLSNGNSEPRRPKITFDDVLHNADYYMQEAKKLKHKADALLEKFGKAVNYADAALSFIECGNAMERDPLEAKSPYTMYSETVELIRYAMRLKNFTGSSATEGDKKLAVLCYRCLSLLYLRMFKLKKDHAMKYSRSLMEYFKNSSKASQAPSPWGGNGKSTETPSPMPLSPSPVSSAGGSAGITGSSSAGTITIPQRIHHMAASHVNITNNVLRSYEHWDMADKLTKENKEFFVDLDTVMGPLTQHSSMTNLVRYVRQGLHWLHIEAHLL

Target ORF information:

RefSeq Version	XM_015278653.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus AF4/FMR2 family member 2 (AFF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015278653.2

ORF Insert Sequence:

ATGTGTATGT GTGCGCGCGC CAGGGCCGCT GCCGCCGCTG TGCCCGCCTC TCCCAGCCAG 
GCAGCAGCCG CGCGCGCTGC TGGCGGCGGG CGGCGGCGGC GGCGCACCAC GAGCCCCGGG 
CGGCTCCGCG GCGGCCGCCG CGGCCGCCGC TGCCCGTGCC CCTGCCCCTG CCGCGCTGCC 
TCTGCTCGGC TCGGCGCGGG GAGAGGGTAC CACTTCTTCG AGTCTCCGCA CCAGATCGTA 
ATGGACCTAT TCGACTTTTT CAGGGACTGG GACTTAGAGC AGCAGTGTCA CTATGAGCAA 
GACCGGAATG CACTTAAGCG AAAGGAATGG GAGAGAAGAA ACCAAGAAGT CCAGCAAGAT 
GAAGACCTCT TTGCTTCAGG TTTTAATCTC TTTGGGGAAC CCTACAAGAC AAATAAAGGT 
GATGCTCTTG CAAACCGTGT CCAGAACACA TTGGGAAACT ATGATGAGAT GAAGGATCTG 
CTAACTAACC ATTCCAATCA GAGCCATCTT GTAGGAATCC CAAAGAATTC TGTCCCACAG 
ACACCCATCG AGAAAAATGA ACAGAACTTT TTCTCAGAAC AAAGAAACAG GGTGATCCCA 
TCTCATCAGA TCAGTGGCCA CTCATCGACA TCAATGCCTC CACCTTCGTC ACTGTCATCT 
AATTCTGCTT TGCTGCACAG TCACCAGAGC AGCAGGAAGT CGAGAACAGA CTGGTCACGC 
AGTGGTCACA ACTGTAGTGG GACCCAGCAA AGCCAATCCA GTAGTCAGCA AAGTCGGACA 
AAGCATAGCT CTTCTCATGA CCTGCCACAA GGCAGATATG AAGACCTCTA TAATTGTCAG 
AGTGAGCAGC ATAAAGGTGG AGGAACTGAG GAAGCAAATT CTATGGCAGC ATCTTCACAT 
TCAAGGCGGC ATACTCATTC CAAGTCAGCA GCTGGAGAAC ATACTTACAA AGAAAACAGT 
CATTCAAAGT CACCCACGGA GCTTGAGTTT GTAGGTCATG GTCCTGGATC TCCACTTCCT 
TCCACATCTT TGTTGTCTTC AAACAATGGT CTTTCAAGCC AGAATTTCCC ACCAGGACTT 
CACTGTAAAA GCAGCATGGT CCAGCAAAAG CCTACAGCAT ATGTTAGGCC GATGGATGGT 
CAGGACCAGG TACCGAATGA CTCACCTGAA CTGAAACCTC CCATAGAAAT TGAGAGTGGA 
TATGGAAATC AGTCCTTTGG AACACTGTTG GAAGGAAAAG TGAACACACC TAGTTCAAAA 
AATAAAGTAC CAAAGCTTAA TATTCCTCCT GTTGGTGAAG CAATGGGAGT TGTGCATTGG 
ATCAGCAACA GCGTAAGTGG ATCGAGGAGT GCAGCAAGCT GTGGATCGAA GGTTACCCTT 
CCCAATGACT CCAGCAGTGT GGAAGAAATA CTGCGTGAGA TGTCCCATTC CTGGCCTCCT 
CCTCTCACTG CTATTCACAC TCCTGGAAAG GCTGAACAGA CCAAGTTTTC TATCCCAAGC 
AAAGTACAAG ACTCCCAACA TCTGACCTCA GGATACAATG TACAAAAGTG GAGTGATCCA 
GGAGGGAAAG TTGCAACTAA GTCAGTGACA CAAAAGTCGA TGCTTGAGGA TGATCTGAAA 
CTCAGCAGTG ATGAAGATGA CAATGAACAG GCTGCTGAAA AGACAAAATT GAGAAACATT 
CCTGTAAACG GCCTGCCGGT GTCGGTGGCA CATGCAGCTG GCTTAGCCCA TTCCAGTGGT 
GGCTCTGGGA GCTCCAGTGA GTCCGAGAGC AGCTCTGAGT CTGACTCCGA CAGTGAGAGC 
AGCTCCAGTG ACAGCGACTG CAATGAGGAA TCACGCAGTG CCACGCCAGA GCCTGAACCT 
CCCTCAACAA ACAAATGGCA GCTGGATAAG TGGCTAAATA AAGTGAACCC CCACAACAAG 
ACCATCATCA GCAATCAAAA TGAGCCTCAC AGTGAGACCA GCTCCCAGCC ACAGAACAGC 
CAGCAGGCTG AAGGGCAAAA CAAAGGGAAG CTCAGCAACA GCCTGCCCCC AGCTGATTCC 
AAAGACCAGG CGATCCTCAG TCCCATCCGA GAGAAAGCCA AACCACGGGT TGCCCAGAAA 
ACTCCAGAGG CAAAAAGCTC CAAGCAGAAG TCGCCAGCTC ATAATGAGCC AACTGCACAA 
AGGACTACTG GGAAAAAGCA ACCCAAAAAG GTAGAAAGAA CTTCCAGTGT AGAAGAGTAT 
AACTGGCCCA AACCAAATAA TACCAGCAAC ACTCCCAAAG AAAAAGACAC ACAGGAACCC 
CCCGAGCAGC CAAAGGTTCG AACTAAAGCA GCAGTTGGCA AGACTGCCCC AAGGAAGGAA 
CCTCGAACAA GCACAGGTCT CACTTCGGAG AAGAAAAAGT ACAGAGGCCC CAGCAAAATT 
GTCCCCAAGT CCCGAGAATT TATTGAAACT GATTCATCTA CTTCTGACTC AAATACAGAT 
CAGGAGGAGA GCCTGCAGGC TAAGATATTG CCAGCTTGTA GTGGACCAGC CAACAGCATC 
AAAGTGAAGG ACCCTGGCAG TAACATCCTC AGTGTAAGTA GCTTAACTAG CAATGGCAGC 
AACACATCCA TCGACCAGAC TAGCAATGAC TTGGAGGAGC AGCTCTTTTC TCCTATCCCA 
ATTGTACAAA ATGAACTTCT GTCCCCACTG AGAGAATATG AAGAACTCAA ATCTCTTTGG 
GTGAAAATAG ACCTTAGTTT ACTCTCTAGA ATACCTGGAC AGGAGCCTTT TGAGACCATA 
TCTGTGAAAA CTGAACCAAG AGAGGCAAAT GTGAAACACA GACGACCATC TCGAGAGTCC 
CCTACCCCAG CAGCTGAAAA AACATCTGCA AAATCCAAGA GGAAACACAA GTCGGAAAAC 
CTGGAAACCT TTGTTGAAAC CAAGAAGCAG CGCCTGGAGG ACCCTTCAGC TTCGTGCTTG 
CTCCCACCTT GTATCTCTCC AATACCAAAC CACAGATTAA CCAGCACTAA AGAGAGCAGC 
AGTAACTTGG TGAAGAAGAT GGCAAAGAAA AGAGAAGAGA AGCTCTTCCC TCCTCCATTA 
TCCCCCCTGT TGGATGAGCC TGTGCACCGT CGGCACAGCA ATGACAACAG CTCCTTCAGC 
CAAGAAACCA GTATCACAAA CACCTGTCAG ACCAGCAGCT CTTCCTCCTC AGTTTCTAAA 
CACAGAAAGA ATGAAAATAA ATCTAACCCC AAAGGAATCT GTGAGGAAGA AACCCACAAT 
GCACCAACAG CCAATGCTCT TCTTGATACG AACCATCTAG AAACAGATAT CTGGTCTGCA 
ATGCCAACTC TTTCCAACGG GAATTCTGAG CCCAGAAGAC CTAAAATCAC ATTTGATGAT 
GTGCTTCACA ATGCAGATTA TTATATGCAA GAAGCGAAGA AGCTAAAGCA TAAAGCGGAT 
GCATTGCTGG AGAAGTTCGG CAAAGCAGTG AATTATGCTG ATGCTGCCTT GTCCTTCATC 
GAGTGTGGGA ATGCTATGGA GCGAGATCCA TTAGAAGCTA AATCTCCATA CACCATGTAC 
TCAGAGACTG TGGAACTCAT CAGGTATGCA ATGAGACTCA AGAATTTCAC AGGCTCTTCT 
GCCACAGAAG GGGACAAGAA ACTAGCAGTT TTATGCTACC GATGCTTATC GCTTCTGTAC 
TTGAGAATGT TTAAACTAAA GAAGGACCAT GCTATGAAGT ACTCCAGATC TCTGATGGAA 
TATTTTAAGA ACTCTTCAAA AGCCTCACAG GCCCCCTCAC CTTGGGGCGG CAATGGAAAG 
AGCACAGAAA CACCATCACC CATGCCCCTG AGCCCATCCC CTGTCAGCTC TGCTGGAGGC 
AGTGCAGGGA TCACTGGCTC CTCCTCAGCA GGGACCATCA CCATTCCTCA GCGCATTCAC 
CACATGGCTG CCAGCCATGT CAACATCACC AACAATGTTC TGCGAAGCTA CGAGCACTGG 
GACATGGCTG ACAAGCTGAC TAAGGAGAAT AAAGAATTCT TTGTGGACCT GGACACAGTG 
ATGGGACCCT TAACACAACA CAGCAGTATG ACCAATCTGG TTCGTTACGT TCGCCAAGGA 
CTCCACTGGC TCCACATTGA GGCTCATTTG TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF2 ( XM_025150464.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025006232.1 Gallus gallus AF4/FMR2 family member 2 (AFF2), transcript variant X2, mRNA.

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CloneID	OGa150689
Clone ID Related Accession (Same CDS sequence)	XM_025150464.1
Accession Version	XM_025150464.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	AF4/FMR2 family member 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTATGT GTGCGCGCGC CAGGGCCGCT GCCGCCGCTG TGCCCGCCTC TCCCAGCCAG 
GCAGCAGCCG CGCGCGCTGC TGGCGGCGGG CGGCGGCGGC GGCGCACCAC GAGCCCCGGG 
CGGCTCCGCG GCGGCCGCCG CGGCCGCCGC TGCCCGTGCC CCTGCCCCTG CCGCGCTGCC 
TCTGCTCGGC TCGGCGCGGG GAGAGGGTAC CACTTCTTCG AGTCTCCGCA CCAGATCGTA 
ATGGACCTAT TCGACTTTTT CAGGGACTGG GACTTAGAGC AGCAGTGTCA CTATGAGCAA 
GACCGGAATG CACTTAAGCG AAAGGAATGG GAGAGAAGAA ACCAAGAAGT CCAGCAAGAT 
GAAGACCTCT TTGCTTCAGG TTTTAATCTC TTTGGGGAAC CCTACAAGAC AAATAAAGGT 
GATGCTCTTG CAAACCGTGT CCAGAACACA TTGGGAAACT ATGATGAGAT GAAGGATCTG 
CTAACTAACC ATTCCAATCA GAGCCATCTT GTAGGAATCC CAAAGAATTC TGTCCCACAG 
ACACCCATCG AGAAAAATGA ACAGAACTTT TTCTCAGAAC AAAGAAACAG GGTGATCCCA 
TCTCATCAGA TCAGTGGCCA CTCATCGACA TCAATGCCTC CACCTTCGTC ACTGTCATCT 
AATTCTGCTT TGCTGCACAG TCACCAGAGC AGCAGGAAGT CGAGAACAGA CTGGTCACGC 
AGTGGTCACA ACTGTAGTGG GACCCAGCAA AGCCAATCCA GTAGTCAGCA AAGTCGGACA 
AAGCATAGCT CTTCTCATGA CCTGCCACAA GGCAGATATG AAGACCTCTA TAATTGTCAG 
AGTGAGCAGC ATAAAGGTGG AGGAACTGAG GAAGCAAATT CTATGGCAGC ATCTTCACAT 
TCAAGGCGGC ATACTCATTC CAAGTCAGCA GCTGGAGAAC ATACTTACAA AGAAAACAGT 
CATTCAAAGT CACCCACGGA GCTTGAGTTT GTAGGTCATG GTCCTGGATC TCCACTTCCT 
TCCACATCTT TGTTGTCTTC AAACAATGGT CTTTCAAGCC AGAATTTCCC ACCAGGACTT 
CACTGTAAAA GCAGCATGGT CCAGCAAAAG CCTACAGCAT ATGTTAGGCC GATGGATGGT 
CAGGACCAGG TACCGAATGA CTCACCTGAA CTGAAACCTC CCATAGAAAT TGAGAGTGGA 
TATGGAAATC AGTCCTTTGG AACACTGTTG GAAGGAAAAG TGAACACACC TAGTTCAAAA 
AATAAAGTAC CAAAGCTTAA TATTCCTCCT GTTGGTGAAG TTACCCTTCC CAATGACTCC 
AGCAGTGTGG AAGAAATACT GCGTGAGATG TCCCATTCCT GGCCTCCTCC TCTCACTGCT 
ATTCACACTC CTGGAAAGGC TGAACAGACC AAGTTTTCTA TCCCAAGCAA AGTACAAGAC 
TCCCAACATC TGACCTCAGG ATACAATGTA CAAAAGTGGA GTGATCCAGG AGGGAAAGTT 
GCAACTAAGT CAGTGACACA AAAGTCGATG CTTGAGGATG ATCTGAAACT CAGCAGTGAT 
GAAGATGACA ATGAACAGGC TGCTGAAAAG ACAAAATTGA GAAACATTCC TGTAAACGGC 
CTGCCGGTGT CGGTGGCACA TGCAGCTGGC TTAGCCCATT CCAGTGGTGG CTCTGGGAGC 
TCCAGTGAGT CCGAGAGCAG CTCTGAGTCT GACTCCGACA GTGAGAGCAG CTCCAGTGAC 
AGCGACTGCA ATGAGGAATC ACGCAGTGCC ACGCCAGAGC CTGAACCTCC CTCAACAAAC 
AAATGGCAGC TGGATAAGTG GCTAAATAAA GTGAACCCCC ACAACAAGAC CATCATCAGC 
AATCAAAATG AGCCTCACAG TGAGACCAGC TCCCAGCCAC AGAACAGCCA GCAGGCTGAA 
GGGCAAAACA AAGGGAAGCT CAGCAACAGC CTGCCCCCAG CTGATTCCAA AGACCAGGCG 
ATCCTCAGTC CCATCCGAGA GAAAGCCAAA CCACGGGTTG CCCAGAAAAC TCCAGAGGCA 
AAAAGCTCCA AGCAGAAGTC GCCAGCTCAT AATGAGCCAA CTGCACAAAG GACTACTGGG 
AAAAAGCAAC CCAAAAAGGT AGAAAGAACT TCCAGTGTAG AAGAGTATAA CTGGCCCAAA 
CCAAATAATA CCAGCAACAC TCCCAAAGAA AAAGACACAC AGGAACCCCC CGAGCAGCCA 
AAGGTTCGAA CTAAAGCAGC AGTTGGCAAG ACTGCCCCAA GGAAGGAACC TCGAACAAGC 
ACAGGTCTCA CTTCGGAGAA GAAAAAGTAC AGAGGCCCCA GCAAAATTGT CCCCAAGTCC 
CGAGAATTTA TTGAAACTGA TTCATCTACT TCTGACTCAA ATACAGATCA GGAGGAGAGC 
CTGCAGGCTA AGATATTGCC AGCTTGTAGT GGACCAGCCA ACAGCATCAA AGTGAAGGAC 
CCTGGCAGTA ACATCCTCAG TGTAAGTAGC TTAACTAGCA ATGGCAGCAA CACATCCATC 
GACCAGACTA GCAATGACTT GGAGGAGCAG CTCTTTTCTC CTATCCCAAT TGTACAAAAT 
GAACTTCTGT CCCCACTGAG AGAATATGAA GAACTCAAAT CTCTTTGGGT GAAAATAGAC 
CTTAGTTTAC TCTCTAGAAT ACCTGGACAG GAGCCTTTTG AGACCATATC TGTGAAAACT 
GAACCAAGAG AGGCAAATGT GAAACACAGA CGACCATCTC GAGAGTCCCC TACCCCAGCA 
GCTGAAAAAA CATCTGCAAA ATCCAAGAGG AAACACAAGT CGGAAAACCT GGAAACCTTT 
GTTGAAACCA AGAAGCAGCG CCTGGAGGAC CCTTCAGCTT CGTGCTTGCT CCCACCTTGT 
ATCTCTCCAA TACCAAACCA CAGATTAACC AGCACTAAAG AGAGCAGCAG TAACTTGGTG 
AAGAAGATGG CAAAGAAAAG AGAAGAGAAG CTCTTCCCTC CTCCATTATC CCCCCTGTTG 
GATGAGCCTG TGCACCGTCG GCACAGCAAT GACAACAGCT CCTTCAGCCA AGAAACCAGT 
ATCACAAACA CCTGTCAGAC CAGCAGCTCT TCCTCCTCAG TTTCTAAACA CAGAAAGAAT 
GAAAATAAAT CTAACCCCAA AGGAATCTGT GAGGAAGAAA CCCACAATGC ACCAACAGCC 
AATGCTCTTC TTGATACGAA CCATCTAGAA ACAGATATCT GGTCTGCAAT GCCAACTCTT 
TCCAACGGGA ATTCTGAGCC CAGAAGACCT AAAATCACAT TTGATGATGT GCTTCACAAT 
GCAGATTATT ATATGCAAGA AGCGAAGAAG CTAAAGCATA AAGCGGATGC ATTGCTGGAG 
AAGTTCGGCA AAGCAGTGAA TTATGCTGAT GCTGCCTTGT CCTTCATCGA GTGTGGGAAT 
GCTATGGAGC GAGATCCATT AGAAGCTAAA TCTCCATACA CCATGTACTC AGAGACTGTG 
GAACTCATCA GGTATGCAAT GAGACTCAAG AATTTCACAG GCTCTTCTGC CACAGAAGGG 
GACAAGAAAC TAGCAGTTTT ATGCTACCGA TGCTTATCGC TTCTGTACTT GAGAATGTTT 
AAACTAAAGA AGGACCATGC TATGAAGTAC TCCAGATCTC TGATGGAATA TTTTAAGAAC 
TCTTCAAAAG CCTCACAGGC CCCCTCACCT TGGGGCGGCA ATGGAAAGAG CACAGAAACA 
CCATCACCCA TGCCCCTGAG CCCATCCCCT GTCAGCTCTG CTGGAGGCAG TGCAGGGATC 
ACTGGCTCCT CCTCAGCAGG GACCATCACC ATTCCTCAGC GCATTCACCA CATGGCTGCC 
AGCCATGTCA ACATCACCAA CAATGTTCTG CGAAGCTACG AGCACTGGGA CATGGCTGAC 
AAGCTGACTA AGGAGAATAA AGAATTCTTT GTGGACCTGG ACACAGTGAT GGGACCCTTA 
ACACAACACA GCAGTATGAC CAATCTGGTT CGTTACGTTC GCCAAGGACT CCACTGGCTC 
CACATTGAGG CTCATTTGTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025006232.1
CDS	16..4059
Translation	MCMCARARAAAAAVPASPSQAAAARAAGGGRRRRRTTSPGRLRGGRRGRRCPCPCPCRAASARLGAGRGYHFFESPHQIVMDLFDFFRDWDLEQQCHYEQDRNALKRKEWERRNQEVQQDEDLFASGFNLFGEPYKTNKGDALANRVQNTLGNYDEMKDLLTNHSNQSHLVGIPKNSVPQTPIEKNEQNFFSEQRNRVIPSHQISGHSSTSMPPPSSLSSNSALLHSHQSSRKSRTDWSRSGHNCSGTQQSQSSSQQSRTKHSSSHDLPQGRYEDLYNCQSEQHKGGGTEEANSMAASSHSRRHTHSKSAAGEHTYKENSHSKSPTELEFVGHGPGSPLPSTSLLSSNNGLSSQNFPPGLHCKSSMVQQKPTAYVRPMDGQDQVPNDSPELKPPIEIESGYGNQSFGTLLEGKVNTPSSKNKVPKLNIPPVGEVTLPNDSSSVEEILREMSHSWPPPLTAIHTPGKAEQTKFSIPSKVQDSQHLTSGYNVQKWSDPGGKVATKSVTQKSMLEDDLKLSSDEDDNEQAAEKTKLRNIPVNGLPVSVAHAAGLAHSSGGSGSSSESESSSESDSDSESSSSDSDCNEESRSATPEPEPPSTNKWQLDKWLNKVNPHNKTIISNQNEPHSETSSQPQNSQQAEGQNKGKLSNSLPPADSKDQAILSPIREKAKPRVAQKTPEAKSSKQKSPAHNEPTAQRTTGKKQPKKVERTSSVEEYNWPKPNNTSNTPKEKDTQEPPEQPKVRTKAAVGKTAPRKEPRTSTGLTSEKKKYRGPSKIVPKSREFIETDSSTSDSNTDQEESLQAKILPACSGPANSIKVKDPGSNILSVSSLTSNGSNTSIDQTSNDLEEQLFSPIPIVQNELLSPLREYEELKSLWVKIDLSLLSRIPGQEPFETISVKTEPREANVKHRRPSRESPTPAAEKTSAKSKRKHKSENLETFVETKKQRLEDPSASCLLPPCISPIPNHRLTSTKESSSNLVKKMAKKREEKLFPPPLSPLLDEPVHRRHSNDNSSFSQETSITNTCQTSSSSSSVSKHRKNENKSNPKGICEEETHNAPTANALLDTNHLETDIWSAMPTLSNGNSEPRRPKITFDDVLHNADYYMQEAKKLKHKADALLEKFGKAVNYADAALSFIECGNAMERDPLEAKSPYTMYSETVELIRYAMRLKNFTGSSATEGDKKLAVLCYRCLSLLYLRMFKLKKDHAMKYSRSLMEYFKNSSKASQAPSPWGGNGKSTETPSPMPLSPSPVSSAGGSAGITGSSSAGTITIPQRIHHMAASHVNITNNVLRSYEHWDMADKLTKENKEFFVDLDTVMGPLTQHSSMTNLVRYVRQGLHWLHIEAHLL

Target ORF information:

RefSeq Version	XM_025150464.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus AF4/FMR2 family member 2 (AFF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025150464.1

ORF Insert Sequence:

ATGTGTATGT GTGCGCGCGC CAGGGCCGCT GCCGCCGCTG TGCCCGCCTC TCCCAGCCAG 
GCAGCAGCCG CGCGCGCTGC TGGCGGCGGG CGGCGGCGGC GGCGCACCAC GAGCCCCGGG 
CGGCTCCGCG GCGGCCGCCG CGGCCGCCGC TGCCCGTGCC CCTGCCCCTG CCGCGCTGCC 
TCTGCTCGGC TCGGCGCGGG GAGAGGGTAC CACTTCTTCG AGTCTCCGCA CCAGATCGTA 
ATGGACCTAT TCGACTTTTT CAGGGACTGG GACTTAGAGC AGCAGTGTCA CTATGAGCAA 
GACCGGAATG CACTTAAGCG AAAGGAATGG GAGAGAAGAA ACCAAGAAGT CCAGCAAGAT 
GAAGACCTCT TTGCTTCAGG TTTTAATCTC TTTGGGGAAC CCTACAAGAC AAATAAAGGT 
GATGCTCTTG CAAACCGTGT CCAGAACACA TTGGGAAACT ATGATGAGAT GAAGGATCTG 
CTAACTAACC ATTCCAATCA GAGCCATCTT GTAGGAATCC CAAAGAATTC TGTCCCACAG 
ACACCCATCG AGAAAAATGA ACAGAACTTT TTCTCAGAAC AAAGAAACAG GGTGATCCCA 
TCTCATCAGA TCAGTGGCCA CTCATCGACA TCAATGCCTC CACCTTCGTC ACTGTCATCT 
AATTCTGCTT TGCTGCACAG TCACCAGAGC AGCAGGAAGT CGAGAACAGA CTGGTCACGC 
AGTGGTCACA ACTGTAGTGG GACCCAGCAA AGCCAATCCA GTAGTCAGCA AAGTCGGACA 
AAGCATAGCT CTTCTCATGA CCTGCCACAA GGCAGATATG AAGACCTCTA TAATTGTCAG 
AGTGAGCAGC ATAAAGGTGG AGGAACTGAG GAAGCAAATT CTATGGCAGC ATCTTCACAT 
TCAAGGCGGC ATACTCATTC CAAGTCAGCA GCTGGAGAAC ATACTTACAA AGAAAACAGT 
CATTCAAAGT CACCCACGGA GCTTGAGTTT GTAGGTCATG GTCCTGGATC TCCACTTCCT 
TCCACATCTT TGTTGTCTTC AAACAATGGT CTTTCAAGCC AGAATTTCCC ACCAGGACTT 
CACTGTAAAA GCAGCATGGT CCAGCAAAAG CCTACAGCAT ATGTTAGGCC GATGGATGGT 
CAGGACCAGG TACCGAATGA CTCACCTGAA CTGAAACCTC CCATAGAAAT TGAGAGTGGA 
TATGGAAATC AGTCCTTTGG AACACTGTTG GAAGGAAAAG TGAACACACC TAGTTCAAAA 
AATAAAGTAC CAAAGCTTAA TATTCCTCCT GTTGGTGAAG TTACCCTTCC CAATGACTCC 
AGCAGTGTGG AAGAAATACT GCGTGAGATG TCCCATTCCT GGCCTCCTCC TCTCACTGCT 
ATTCACACTC CTGGAAAGGC TGAACAGACC AAGTTTTCTA TCCCAAGCAA AGTACAAGAC 
TCCCAACATC TGACCTCAGG ATACAATGTA CAAAAGTGGA GTGATCCAGG AGGGAAAGTT 
GCAACTAAGT CAGTGACACA AAAGTCGATG CTTGAGGATG ATCTGAAACT CAGCAGTGAT 
GAAGATGACA ATGAACAGGC TGCTGAAAAG ACAAAATTGA GAAACATTCC TGTAAACGGC 
CTGCCGGTGT CGGTGGCACA TGCAGCTGGC TTAGCCCATT CCAGTGGTGG CTCTGGGAGC 
TCCAGTGAGT CCGAGAGCAG CTCTGAGTCT GACTCCGACA GTGAGAGCAG CTCCAGTGAC 
AGCGACTGCA ATGAGGAATC ACGCAGTGCC ACGCCAGAGC CTGAACCTCC CTCAACAAAC 
AAATGGCAGC TGGATAAGTG GCTAAATAAA GTGAACCCCC ACAACAAGAC CATCATCAGC 
AATCAAAATG AGCCTCACAG TGAGACCAGC TCCCAGCCAC AGAACAGCCA GCAGGCTGAA 
GGGCAAAACA AAGGGAAGCT CAGCAACAGC CTGCCCCCAG CTGATTCCAA AGACCAGGCG 
ATCCTCAGTC CCATCCGAGA GAAAGCCAAA CCACGGGTTG CCCAGAAAAC TCCAGAGGCA 
AAAAGCTCCA AGCAGAAGTC GCCAGCTCAT AATGAGCCAA CTGCACAAAG GACTACTGGG 
AAAAAGCAAC CCAAAAAGGT AGAAAGAACT TCCAGTGTAG AAGAGTATAA CTGGCCCAAA 
CCAAATAATA CCAGCAACAC TCCCAAAGAA AAAGACACAC AGGAACCCCC CGAGCAGCCA 
AAGGTTCGAA CTAAAGCAGC AGTTGGCAAG ACTGCCCCAA GGAAGGAACC TCGAACAAGC 
ACAGGTCTCA CTTCGGAGAA GAAAAAGTAC AGAGGCCCCA GCAAAATTGT CCCCAAGTCC 
CGAGAATTTA TTGAAACTGA TTCATCTACT TCTGACTCAA ATACAGATCA GGAGGAGAGC 
CTGCAGGCTA AGATATTGCC AGCTTGTAGT GGACCAGCCA ACAGCATCAA AGTGAAGGAC 
CCTGGCAGTA ACATCCTCAG TGTAAGTAGC TTAACTAGCA ATGGCAGCAA CACATCCATC 
GACCAGACTA GCAATGACTT GGAGGAGCAG CTCTTTTCTC CTATCCCAAT TGTACAAAAT 
GAACTTCTGT CCCCACTGAG AGAATATGAA GAACTCAAAT CTCTTTGGGT GAAAATAGAC 
CTTAGTTTAC TCTCTAGAAT ACCTGGACAG GAGCCTTTTG AGACCATATC TGTGAAAACT 
GAACCAAGAG AGGCAAATGT GAAACACAGA CGACCATCTC GAGAGTCCCC TACCCCAGCA 
GCTGAAAAAA CATCTGCAAA ATCCAAGAGG AAACACAAGT CGGAAAACCT GGAAACCTTT 
GTTGAAACCA AGAAGCAGCG CCTGGAGGAC CCTTCAGCTT CGTGCTTGCT CCCACCTTGT 
ATCTCTCCAA TACCAAACCA CAGATTAACC AGCACTAAAG AGAGCAGCAG TAACTTGGTG 
AAGAAGATGG CAAAGAAAAG AGAAGAGAAG CTCTTCCCTC CTCCATTATC CCCCCTGTTG 
GATGAGCCTG TGCACCGTCG GCACAGCAAT GACAACAGCT CCTTCAGCCA AGAAACCAGT 
ATCACAAACA CCTGTCAGAC CAGCAGCTCT TCCTCCTCAG TTTCTAAACA CAGAAAGAAT 
GAAAATAAAT CTAACCCCAA AGGAATCTGT GAGGAAGAAA CCCACAATGC ACCAACAGCC 
AATGCTCTTC TTGATACGAA CCATCTAGAA ACAGATATCT GGTCTGCAAT GCCAACTCTT 
TCCAACGGGA ATTCTGAGCC CAGAAGACCT AAAATCACAT TTGATGATGT GCTTCACAAT 
GCAGATTATT ATATGCAAGA AGCGAAGAAG CTAAAGCATA AAGCGGATGC ATTGCTGGAG 
AAGTTCGGCA AAGCAGTGAA TTATGCTGAT GCTGCCTTGT CCTTCATCGA GTGTGGGAAT 
GCTATGGAGC GAGATCCATT AGAAGCTAAA TCTCCATACA CCATGTACTC AGAGACTGTG 
GAACTCATCA GGTATGCAAT GAGACTCAAG AATTTCACAG GCTCTTCTGC CACAGAAGGG 
GACAAGAAAC TAGCAGTTTT ATGCTACCGA TGCTTATCGC TTCTGTACTT GAGAATGTTT 
AAACTAAAGA AGGACCATGC TATGAAGTAC TCCAGATCTC TGATGGAATA TTTTAAGAAC 
TCTTCAAAAG CCTCACAGGC CCCCTCACCT TGGGGCGGCA ATGGAAAGAG CACAGAAACA 
CCATCACCCA TGCCCCTGAG CCCATCCCCT GTCAGCTCTG CTGGAGGCAG TGCAGGGATC 
ACTGGCTCCT CCTCAGCAGG GACCATCACC ATTCCTCAGC GCATTCACCA CATGGCTGCC 
AGCCATGTCA ACATCACCAA CAATGTTCTG CGAAGCTACG AGCACTGGGA CATGGCTGAC 
AAGCTGACTA AGGAGAATAA AGAATTCTTT GTGGACCTGG ACACAGTGAT GGGACCCTTA 
ACACAACACA GCAGTATGAC CAATCTGGTT CGTTACGTTC GCCAAGGACT CCACTGGCTC 
CACATTGAGG CTCATTTGTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF2 ( XM_025150465.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025006233.1 Gallus gallus AF4/FMR2 family member 2 (AFF2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa150690
Clone ID Related Accession (Same CDS sequence)	XM_025150465.1
Accession Version	XM_025150465.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	AF4/FMR2 family member 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTATGT GTGCGCGCGC CAGGGCCGCT GCCGCCGCTG TGCCCGCCTC TCCCAGCCAG 
GCAGCAGCCG CGCGCGCTGC TGGCGGCGGG CGGCGGCGGC GGCGCACCAC GAGCCCCGGG 
CGGCTCCGCG GCGGCCGCCG CGGCCGCCGC TGCCCGTGCC CCTGCCCCTG CCGCGCTGCC 
TCTGCTCGGC TCGGCGCGGG GAGAGGGTAC CACTTCTTCG AGTCTCCGCA CCAGATCGTA 
ATGGACCTAT TCGACTTTTT CAGGGACTGG GACTTAGAGC AGCAGTGTCA CTATGAGCAA 
GACCGGAATG CACTTAAGCG AAAGGAATGG GAGAGAAGAA ACCAAGAAGT CCAGCAAGAT 
GAAGACCTCT TTGCTTCAGG TTTTAATCTC TTTGGGGAAC CCTACAAGAC AAATAAAGGT 
GATGCTCTTG CAAACCGTGT CCAGAACACA TTGGGAAACT ATGATGAGAT GAAGGATCTG 
CTAACTAACC ATTCCAATCA GAGCCATCTT GTAGGAATCC CAAAGAATTC TGTCCCACAG 
ACACCCATCG AGAAAAATGA ACAGAACTTT TTCTCAGAAC AAAGAAACAG GGTGATCCCA 
TCTCATCAGA TCAGTGGCCA CTCATCGACA TCAATGCCTC CACCTTCGTC ACTGTCATCT 
AATTCTGCTT TGCTGCACAG TCACCAGAGC AGCAGGAAGT CGAGAACAGA CTGGTCACGC 
AGTGGTCACA ACTGTAGTGG GACCCAGCAA AGCCAATCCA GTAGTCAGCA AAGTCGGACA 
AAGCATAGCT CTTCTCATGA CCTGCCACAA GGCAGATATG AAGACCTCTA TAATTGTCAG 
AGTGAGCAGC ATAAAGGTGG AGGAACTGAG GAAGCAAATT CTATGGCAGC ATCTTCACAT 
TCAAGGCGGC ATACTCATTC CAAGTCAGCA GCTGGAGAAC ATACTTACAA AGAAAACAGT 
CATTCAAAGT CACCCACGGA GCTTGAGTTT GTAGGTCATG GTCCTGGATC TCCACTTCCT 
TCCACATCTT TGTTGTCTTC AAACAATGGT CTTTCAAGCC AGAATTTCCC ACCAGGACTT 
CACTGTAAAA GCAGCATGGT CCAGCAAAAG CCTACAGCAT ATGTTAGGCC GATGGATGGT 
CAGGACCAGG TACCGAATGA CTCACCTGAA CTGAAACCTC CCATAGAAAT TGAGAGTGGA 
TATGGAAATC AGTCCTTTGG AACACTGTTG GAAGGAAAAG TGAACACACC TAGTTCAAAA 
AATAAAGTAC CAAAGCTTAA TATTCCTCCT GTTGGTGAAG CAATGGGAGT TGTGCATTGG 
ATCAGCAACA GCGTAAGTGG ATCGAGGAGT GCAGCAAGCT GTGGATCGAA GGTTACCCTT 
CCCAATGACT CCAGCAGTGT GGAAGAAATA CTGCGTGACT CCCAACATCT GACCTCAGGA 
TACAATGTAC AAAAGTGGAG TGATCCAGGA GGGAAAGTTG CAACTAAGTC AGTGACACAA 
AAGTCGATGC TTGAGGATGA TCTGAAACTC AGCAGTGATG AAGATGACAA TGAACAGGCT 
GCTGAAAAGA CAAAATTGAG AAACATTCCT GTAAACGGCC TGCCGGTGTC GGTGGCACAT 
GCAGCTGGCT TAGCCCATTC CAGTGGTGGC TCTGGGAGCT CCAGTGAGTC CGAGAGCAGC 
TCTGAGTCTG ACTCCGACAG TGAGAGCAGC TCCAGTGACA GCGACTGCAA TGAGGAATCA 
CGCAGTGCCA CGCCAGAGCC TGAACCTCCC TCAACAAACA AATGGCAGCT GGATAAGTGG 
CTAAATAAAG TGAACCCCCA CAACAAGACC ATCATCAGCA ATCAAAATGA GCCTCACAGT 
GAGACCAGCT CCCAGCCACA GAACAGCCAG CAGGCTGAAG GGCAAAACAA AGGGAAGCTC 
AGCAACAGCC TGCCCCCAGC TGATTCCAAA GACCAGGCGA TCCTCAGTCC CATCCGAGAG 
AAAGCCAAAC CACGGGTTGC CCAGAAAACT CCAGAGGCAA AAAGCTCCAA GCAGAAGTCG 
CCAGCTCATA ATGAGCCAAC TGCACAAAGG ACTACTGGGA AAAAGCAACC CAAAAAGGTA 
GAAAGAACTT CCAGTGTAGA AGAGTATAAC TGGCCCAAAC CAAATAATAC CAGCAACACT 
CCCAAAGAAA AAGACACACA GGAACCCCCC GAGCAGCCAA AGGTTCGAAC TAAAGCAGCA 
GTTGGCAAGA CTGCCCCAAG GAAGGAACCT CGAACAAGCA CAGGTCTCAC TTCGGAGAAG 
AAAAAGTACA GAGGCCCCAG CAAAATTGTC CCCAAGTCCC GAGAATTTAT TGAAACTGAT 
TCATCTACTT CTGACTCAAA TACAGATCAG GAGGAGAGCC TGCAGGCTAA GATATTGCCA 
GCTTGTAGTG GACCAGCCAA CAGCATCAAA GTGAAGGACC CTGGCAGTAA CATCCTCAGT 
GTAAGTAGCT TAACTAGCAA TGGCAGCAAC ACATCCATCG ACCAGACTAG CAATGACTTG 
GAGGAGCAGC TCTTTTCTCC TATCCCAATT GTACAAAATG AACTTCTGTC CCCACTGAGA 
GAATATGAAG AACTCAAATC TCTTTGGGTG AAAATAGACC TTAGTTTACT CTCTAGAATA 
CCTGGACAGG AGCCTTTTGA GACCATATCT GTGAAAACTG AACCAAGAGA GGCAAATGTG 
AAACACAGAC GACCATCTCG AGAGTCCCCT ACCCCAGCAG CTGAAAAAAC ATCTGCAAAA 
TCCAAGAGGA AACACAAGTC GGAAAACCTG GAAACCTTTG TTGAAACCAA GAAGCAGCGC 
CTGGAGGACC CTTCAGCTTC GTGCTTGCTC CCACCTTGTA TCTCTCCAAT ACCAAACCAC 
AGATTAACCA GCACTAAAGA GAGCAGCAGT AACTTGGTGA AGAAGATGGC AAAGAAAAGA 
GAAGAGAAGC TCTTCCCTCC TCCATTATCC CCCCTGTTGG ATGAGCCTGT GCACCGTCGG 
CACAGCAATG ACAACAGCTC CTTCAGCCAA GAAACCAGTA TCACAAACAC CTGTCAGACC 
AGCAGCTCTT CCTCCTCAGT TTCTAAACAC AGAAAGAATG AAAATAAATC TAACCCCAAA 
GGAATCTGTG AGGAAGAAAC CCACAATGCA CCAACAGCCA ATGCTCTTCT TGATACGAAC 
CATCTAGAAA CAGATATCTG GTCTGCAATG CCAACTCTTT CCAACGGGAA TTCTGAGCCC 
AGAAGACCTA AAATCACATT TGATGATGTG CTTCACAATG CAGATTATTA TATGCAAGAA 
GCGAAGAAGC TAAAGCATAA AGCGGATGCA TTGCTGGAGA AGTTCGGCAA AGCAGTGAAT 
TATGCTGATG CTGCCTTGTC CTTCATCGAG TGTGGGAATG CTATGGAGCG AGATCCATTA 
GAAGCTAAAT CTCCATACAC CATGTACTCA GAGACTGTGG AACTCATCAG GTATGCAATG 
AGACTCAAGA ATTTCACAGG CTCTTCTGCC ACAGAAGGGG ACAAGAAACT AGCAGTTTTA 
TGCTACCGAT GCTTATCGCT TCTGTACTTG AGAATGTTTA AACTAAAGAA GGACCATGCT 
ATGAAGTACT CCAGATCTCT GATGGAATAT TTTAAGAACT CTTCAAAAGC CTCACAGGCC 
CCCTCACCTT GGGGCGGCAA TGGAAAGAGC ACAGAAACAC CATCACCCAT GCCCCTGAGC 
CCATCCCCTG TCAGCTCTGC TGGAGGCAGT GCAGGGATCA CTGGCTCCTC CTCAGCAGGG 
ACCATCACCA TTCCTCAGCG CATTCACCAC ATGGCTGCCA GCCATGTCAA CATCACCAAC 
AATGTTCTGC GAAGCTACGA GCACTGGGAC ATGGCTGACA AGCTGACTAA GGAGAATAAA 
GAATTCTTTG TGGACCTGGA CACAGTGATG GGACCCTTAA CACAACACAG CAGTATGACC 
AATCTGGTTC GTTACGTTCG CCAAGGACTC CACTGGCTCC ACATTGAGGC TCATTTGTTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025006233.1
CDS	16..4038
Translation	MCMCARARAAAAAVPASPSQAAAARAAGGGRRRRRTTSPGRLRGGRRGRRCPCPCPCRAASARLGAGRGYHFFESPHQIVMDLFDFFRDWDLEQQCHYEQDRNALKRKEWERRNQEVQQDEDLFASGFNLFGEPYKTNKGDALANRVQNTLGNYDEMKDLLTNHSNQSHLVGIPKNSVPQTPIEKNEQNFFSEQRNRVIPSHQISGHSSTSMPPPSSLSSNSALLHSHQSSRKSRTDWSRSGHNCSGTQQSQSSSQQSRTKHSSSHDLPQGRYEDLYNCQSEQHKGGGTEEANSMAASSHSRRHTHSKSAAGEHTYKENSHSKSPTELEFVGHGPGSPLPSTSLLSSNNGLSSQNFPPGLHCKSSMVQQKPTAYVRPMDGQDQVPNDSPELKPPIEIESGYGNQSFGTLLEGKVNTPSSKNKVPKLNIPPVGEAMGVVHWISNSVSGSRSAASCGSKVTLPNDSSSVEEILRDSQHLTSGYNVQKWSDPGGKVATKSVTQKSMLEDDLKLSSDEDDNEQAAEKTKLRNIPVNGLPVSVAHAAGLAHSSGGSGSSSESESSSESDSDSESSSSDSDCNEESRSATPEPEPPSTNKWQLDKWLNKVNPHNKTIISNQNEPHSETSSQPQNSQQAEGQNKGKLSNSLPPADSKDQAILSPIREKAKPRVAQKTPEAKSSKQKSPAHNEPTAQRTTGKKQPKKVERTSSVEEYNWPKPNNTSNTPKEKDTQEPPEQPKVRTKAAVGKTAPRKEPRTSTGLTSEKKKYRGPSKIVPKSREFIETDSSTSDSNTDQEESLQAKILPACSGPANSIKVKDPGSNILSVSSLTSNGSNTSIDQTSNDLEEQLFSPIPIVQNELLSPLREYEELKSLWVKIDLSLLSRIPGQEPFETISVKTEPREANVKHRRPSRESPTPAAEKTSAKSKRKHKSENLETFVETKKQRLEDPSASCLLPPCISPIPNHRLTSTKESSSNLVKKMAKKREEKLFPPPLSPLLDEPVHRRHSNDNSSFSQETSITNTCQTSSSSSSVSKHRKNENKSNPKGICEEETHNAPTANALLDTNHLETDIWSAMPTLSNGNSEPRRPKITFDDVLHNADYYMQEAKKLKHKADALLEKFGKAVNYADAALSFIECGNAMERDPLEAKSPYTMYSETVELIRYAMRLKNFTGSSATEGDKKLAVLCYRCLSLLYLRMFKLKKDHAMKYSRSLMEYFKNSSKASQAPSPWGGNGKSTETPSPMPLSPSPVSSAGGSAGITGSSSAGTITIPQRIHHMAASHVNITNNVLRSYEHWDMADKLTKENKEFFVDLDTVMGPLTQHSSMTNLVRYVRQGLHWLHIEAHLL

Target ORF information:

RefSeq Version	XM_025150465.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus AF4/FMR2 family member 2 (AFF2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025150465.1

ORF Insert Sequence:

ATGTGTATGT GTGCGCGCGC CAGGGCCGCT GCCGCCGCTG TGCCCGCCTC TCCCAGCCAG 
GCAGCAGCCG CGCGCGCTGC TGGCGGCGGG CGGCGGCGGC GGCGCACCAC GAGCCCCGGG 
CGGCTCCGCG GCGGCCGCCG CGGCCGCCGC TGCCCGTGCC CCTGCCCCTG CCGCGCTGCC 
TCTGCTCGGC TCGGCGCGGG GAGAGGGTAC CACTTCTTCG AGTCTCCGCA CCAGATCGTA 
ATGGACCTAT TCGACTTTTT CAGGGACTGG GACTTAGAGC AGCAGTGTCA CTATGAGCAA 
GACCGGAATG CACTTAAGCG AAAGGAATGG GAGAGAAGAA ACCAAGAAGT CCAGCAAGAT 
GAAGACCTCT TTGCTTCAGG TTTTAATCTC TTTGGGGAAC CCTACAAGAC AAATAAAGGT 
GATGCTCTTG CAAACCGTGT CCAGAACACA TTGGGAAACT ATGATGAGAT GAAGGATCTG 
CTAACTAACC ATTCCAATCA GAGCCATCTT GTAGGAATCC CAAAGAATTC TGTCCCACAG 
ACACCCATCG AGAAAAATGA ACAGAACTTT TTCTCAGAAC AAAGAAACAG GGTGATCCCA 
TCTCATCAGA TCAGTGGCCA CTCATCGACA TCAATGCCTC CACCTTCGTC ACTGTCATCT 
AATTCTGCTT TGCTGCACAG TCACCAGAGC AGCAGGAAGT CGAGAACAGA CTGGTCACGC 
AGTGGTCACA ACTGTAGTGG GACCCAGCAA AGCCAATCCA GTAGTCAGCA AAGTCGGACA 
AAGCATAGCT CTTCTCATGA CCTGCCACAA GGCAGATATG AAGACCTCTA TAATTGTCAG 
AGTGAGCAGC ATAAAGGTGG AGGAACTGAG GAAGCAAATT CTATGGCAGC ATCTTCACAT 
TCAAGGCGGC ATACTCATTC CAAGTCAGCA GCTGGAGAAC ATACTTACAA AGAAAACAGT 
CATTCAAAGT CACCCACGGA GCTTGAGTTT GTAGGTCATG GTCCTGGATC TCCACTTCCT 
TCCACATCTT TGTTGTCTTC AAACAATGGT CTTTCAAGCC AGAATTTCCC ACCAGGACTT 
CACTGTAAAA GCAGCATGGT CCAGCAAAAG CCTACAGCAT ATGTTAGGCC GATGGATGGT 
CAGGACCAGG TACCGAATGA CTCACCTGAA CTGAAACCTC CCATAGAAAT TGAGAGTGGA 
TATGGAAATC AGTCCTTTGG AACACTGTTG GAAGGAAAAG TGAACACACC TAGTTCAAAA 
AATAAAGTAC CAAAGCTTAA TATTCCTCCT GTTGGTGAAG CAATGGGAGT TGTGCATTGG 
ATCAGCAACA GCGTAAGTGG ATCGAGGAGT GCAGCAAGCT GTGGATCGAA GGTTACCCTT 
CCCAATGACT CCAGCAGTGT GGAAGAAATA CTGCGTGACT CCCAACATCT GACCTCAGGA 
TACAATGTAC AAAAGTGGAG TGATCCAGGA GGGAAAGTTG CAACTAAGTC AGTGACACAA 
AAGTCGATGC TTGAGGATGA TCTGAAACTC AGCAGTGATG AAGATGACAA TGAACAGGCT 
GCTGAAAAGA CAAAATTGAG AAACATTCCT GTAAACGGCC TGCCGGTGTC GGTGGCACAT 
GCAGCTGGCT TAGCCCATTC CAGTGGTGGC TCTGGGAGCT CCAGTGAGTC CGAGAGCAGC 
TCTGAGTCTG ACTCCGACAG TGAGAGCAGC TCCAGTGACA GCGACTGCAA TGAGGAATCA 
CGCAGTGCCA CGCCAGAGCC TGAACCTCCC TCAACAAACA AATGGCAGCT GGATAAGTGG 
CTAAATAAAG TGAACCCCCA CAACAAGACC ATCATCAGCA ATCAAAATGA GCCTCACAGT 
GAGACCAGCT CCCAGCCACA GAACAGCCAG CAGGCTGAAG GGCAAAACAA AGGGAAGCTC 
AGCAACAGCC TGCCCCCAGC TGATTCCAAA GACCAGGCGA TCCTCAGTCC CATCCGAGAG 
AAAGCCAAAC CACGGGTTGC CCAGAAAACT CCAGAGGCAA AAAGCTCCAA GCAGAAGTCG 
CCAGCTCATA ATGAGCCAAC TGCACAAAGG ACTACTGGGA AAAAGCAACC CAAAAAGGTA 
GAAAGAACTT CCAGTGTAGA AGAGTATAAC TGGCCCAAAC CAAATAATAC CAGCAACACT 
CCCAAAGAAA AAGACACACA GGAACCCCCC GAGCAGCCAA AGGTTCGAAC TAAAGCAGCA 
GTTGGCAAGA CTGCCCCAAG GAAGGAACCT CGAACAAGCA CAGGTCTCAC TTCGGAGAAG 
AAAAAGTACA GAGGCCCCAG CAAAATTGTC CCCAAGTCCC GAGAATTTAT TGAAACTGAT 
TCATCTACTT CTGACTCAAA TACAGATCAG GAGGAGAGCC TGCAGGCTAA GATATTGCCA 
GCTTGTAGTG GACCAGCCAA CAGCATCAAA GTGAAGGACC CTGGCAGTAA CATCCTCAGT 
GTAAGTAGCT TAACTAGCAA TGGCAGCAAC ACATCCATCG ACCAGACTAG CAATGACTTG 
GAGGAGCAGC TCTTTTCTCC TATCCCAATT GTACAAAATG AACTTCTGTC CCCACTGAGA 
GAATATGAAG AACTCAAATC TCTTTGGGTG AAAATAGACC TTAGTTTACT CTCTAGAATA 
CCTGGACAGG AGCCTTTTGA GACCATATCT GTGAAAACTG AACCAAGAGA GGCAAATGTG 
AAACACAGAC GACCATCTCG AGAGTCCCCT ACCCCAGCAG CTGAAAAAAC ATCTGCAAAA 
TCCAAGAGGA AACACAAGTC GGAAAACCTG GAAACCTTTG TTGAAACCAA GAAGCAGCGC 
CTGGAGGACC CTTCAGCTTC GTGCTTGCTC CCACCTTGTA TCTCTCCAAT ACCAAACCAC 
AGATTAACCA GCACTAAAGA GAGCAGCAGT AACTTGGTGA AGAAGATGGC AAAGAAAAGA 
GAAGAGAAGC TCTTCCCTCC TCCATTATCC CCCCTGTTGG ATGAGCCTGT GCACCGTCGG 
CACAGCAATG ACAACAGCTC CTTCAGCCAA GAAACCAGTA TCACAAACAC CTGTCAGACC 
AGCAGCTCTT CCTCCTCAGT TTCTAAACAC AGAAAGAATG AAAATAAATC TAACCCCAAA 
GGAATCTGTG AGGAAGAAAC CCACAATGCA CCAACAGCCA ATGCTCTTCT TGATACGAAC 
CATCTAGAAA CAGATATCTG GTCTGCAATG CCAACTCTTT CCAACGGGAA TTCTGAGCCC 
AGAAGACCTA AAATCACATT TGATGATGTG CTTCACAATG CAGATTATTA TATGCAAGAA 
GCGAAGAAGC TAAAGCATAA AGCGGATGCA TTGCTGGAGA AGTTCGGCAA AGCAGTGAAT 
TATGCTGATG CTGCCTTGTC CTTCATCGAG TGTGGGAATG CTATGGAGCG AGATCCATTA 
GAAGCTAAAT CTCCATACAC CATGTACTCA GAGACTGTGG AACTCATCAG GTATGCAATG 
AGACTCAAGA ATTTCACAGG CTCTTCTGCC ACAGAAGGGG ACAAGAAACT AGCAGTTTTA 
TGCTACCGAT GCTTATCGCT TCTGTACTTG AGAATGTTTA AACTAAAGAA GGACCATGCT 
ATGAAGTACT CCAGATCTCT GATGGAATAT TTTAAGAACT CTTCAAAAGC CTCACAGGCC 
CCCTCACCTT GGGGCGGCAA TGGAAAGAGC ACAGAAACAC CATCACCCAT GCCCCTGAGC 
CCATCCCCTG TCAGCTCTGC TGGAGGCAGT GCAGGGATCA CTGGCTCCTC CTCAGCAGGG 
ACCATCACCA TTCCTCAGCG CATTCACCAC ATGGCTGCCA GCCATGTCAA CATCACCAAC 
AATGTTCTGC GAAGCTACGA GCACTGGGAC ATGGCTGACA AGCTGACTAA GGAGAATAAA 
GAATTCTTTG TGGACCTGGA CACAGTGATG GGACCCTTAA CACAACACAG CAGTATGACC 
AATCTGGTTC GTTACGTTCG CCAAGGACTC CACTGGCTCC ACATTGAGGC TCATTTGTTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF2 ( XM_025150466.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025006234.1 Gallus gallus AF4/FMR2 family member 2 (AFF2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OGa150691
Clone ID Related Accession (Same CDS sequence)	XM_025150466.1
Accession Version	XM_025150466.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	AF4/FMR2 family member 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTATGT GTGCGCGCGC CAGGGCCGCT GCCGCCGCTG TGCCCGCCTC TCCCAGCCAG 
GCAGCAGCCG CGCGCGCTGC TGGCGGCGGG CGGCGGCGGC GGCGCACCAC GAGCCCCGGG 
CGGCTCCGCG GCGGCCGCCG CGGCCGCCGC TGCCCGTGCC CCTGCCCCTG CCGCGCTGCC 
TCTGCTCGGC TCGGCGCGGG GAGAGGGTAC CACTTCTTCG AGTCTCCGCA CCAGATCGTA 
ATGGACCTAT TCGACTTTTT CAGGGACTGG GACTTAGAGC AGCAGTGTCA CTATGAGCAA 
GACCGGAATG CACTTAAGCG AAAGGAATGG GAGAGAAGAA ACCAAGAAGT CCAGCAAGAT 
GAAGACCTCT TTGCTTCAGG TTTTAATCTC TTTGGGGAAC CCTACAAGAC AAATAAAGGT 
GATGCTCTTG CAAACCGTGT CCAGAACACA TTGGGAAACT ATGATGAGAT GAAGGATCTG 
CTAACTAACC ATTCCAATCA GAGCCATCTT GTAGGAATCC CAAAGAATTC TGTCCCACAG 
ACACCCATCG AGAAAAATGA ACAGAACTTT TTCTCAGAAC AAAGAAACAG GGTGATCCCA 
TCTCATCAGA TCAGTGGCCA CTCATCGACA TCAATGCCTC CACCTTCGTC ACTGTCATCT 
AATTCTGCTT TGCTGCACAG TCACCAGAGC AGCAGGAAGT CGAGAACAGA CTGGTCACGC 
AGTGGTCACA ACTGTAGTGG GACCCAGCAA AGCCAATCCA GTAGTCAGCA AAGTCGGACA 
AAGCATAGCT CTTCTCATGA CCTGCCACAA GGCAGATATG AAGACCTCTA TAATTGTCAG 
AGTGAGCAGC ATAAAGGTGG AGGAACTGAG GAAGCAAATT CTATGGCAGC ATCTTCACAT 
TCAAGGCGGC ATACTCATTC CAAGTCAGCA GCTGGAGAAC ATACTTACAA AGAAAACAGT 
CATTCAAAGT CACCCACGGA GCTTGAGTTT GTAGGTCATG GTCCTGGATC TCCACTTCCT 
TCCACATCTT TGTTGTCTTC AAACAATGGT CTTTCAAGCC AGAATTTCCC ACCAGGACTT 
CACTGTAAAA GCAGCATGGT CCAGCAAAAG CCTACAGCAT ATGTTAGGCC GATGGATGGT 
CAGGACCAGG TACCGAATGA CTCACCTGAA CTGAAACCTC CCATAGAAAT TGAGAGTGGA 
TATGGAAATC AGTCCTTTGG AACACTGTTG GAAGGAAAAG TGAACACACC TAGTTCAAAA 
AATAAAGTAC CAAAGCTTAA TATTCCTCCT GTTGGTGAAG AGATGTCCCA TTCCTGGCCT 
CCTCCTCTCA CTGCTATTCA CACTCCTGGA AAGGCTGAAC AGACCAAGTT TTCTATCCCA 
AGCAAAGTAC AAGACTCCCA ACATCTGACC TCAGGATACA ATGTACAAAA GTGGAGTGAT 
CCAGGAGGGA AAGTTGCAAC TAAGTCAGTG ACACAAAAGT CGATGCTTGA GGATGATCTG 
AAACTCAGCA GTGATGAAGA TGACAATGAA CAGGCTGCTG AAAAGACAAA ATTGAGAAAC 
ATTCCTGTAA ACGGCCTGCC GGTGTCGGTG GCACATGCAG CTGGCTTAGC CCATTCCAGT 
GGTGGCTCTG GGAGCTCCAG TGAGTCCGAG AGCAGCTCTG AGTCTGACTC CGACAGTGAG 
AGCAGCTCCA GTGACAGCGA CTGCAATGAG GAATCACGCA GTGCCACGCC AGAGCCTGAA 
CCTCCCTCAA CAAACAAATG GCAGCTGGAT AAGTGGCTAA ATAAAGTGAA CCCCCACAAC 
AAGACCATCA TCAGCAATCA AAATGAGCCT CACAGTGAGA CCAGCTCCCA GCCACAGAAC 
AGCCAGCAGG CTGAAGGGCA AAACAAAGGG AAGCTCAGCA ACAGCCTGCC CCCAGCTGAT 
TCCAAAGACC AGGCGATCCT CAGTCCCATC CGAGAGAAAG CCAAACCACG GGTTGCCCAG 
AAAACTCCAG AGGCAAAAAG CTCCAAGCAG AAGTCGCCAG CTCATAATGA GCCAACTGCA 
CAAAGGACTA CTGGGAAAAA GCAACCCAAA AAGGTAGAAA GAACTTCCAG TGTAGAAGAG 
TATAACTGGC CCAAACCAAA TAATACCAGC AACACTCCCA AAGAAAAAGA CACACAGGAA 
CCCCCCGAGC AGCCAAAGGT TCGAACTAAA GCAGCAGTTG GCAAGACTGC CCCAAGGAAG 
GAACCTCGAA CAAGCACAGG TCTCACTTCG GAGAAGAAAA AGTACAGAGG CCCCAGCAAA 
ATTGTCCCCA AGTCCCGAGA ATTTATTGAA ACTGATTCAT CTACTTCTGA CTCAAATACA 
GATCAGGAGG AGAGCCTGCA GGCTAAGATA TTGCCAGCTT GTAGTGGACC AGCCAACAGC 
ATCAAAGTGA AGGACCCTGG CAGTAACATC CTCAGTGTAA GTAGCTTAAC TAGCAATGGC 
AGCAACACAT CCATCGACCA GACTAGCAAT GACTTGGAGG AGCAGCTCTT TTCTCCTATC 
CCAATTGTAC AAAATGAACT TCTGTCCCCA CTGAGAGAAT ATGAAGAACT CAAATCTCTT 
TGGGTGAAAA TAGACCTTAG TTTACTCTCT AGAATACCTG GACAGGAGCC TTTTGAGACC 
ATATCTGTGA AAACTGAACC AAGAGAGGCA AATGTGAAAC ACAGACGACC ATCTCGAGAG 
TCCCCTACCC CAGCAGCTGA AAAAACATCT GCAAAATCCA AGAGGAAACA CAAGTCGGAA 
AACCTGGAAA CCTTTGTTGA AACCAAGAAG CAGCGCCTGG AGGACCCTTC AGCTTCGTGC 
TTGCTCCCAC CTTGTATCTC TCCAATACCA AACCACAGAT TAACCAGCAC TAAAGAGAGC 
AGCAGTAACT TGGTGAAGAA GATGGCAAAG AAAAGAGAAG AGAAGCTCTT CCCTCCTCCA 
TTATCCCCCC TGTTGGATGA GCCTGTGCAC CGTCGGCACA GCAATGACAA CAGCTCCTTC 
AGCCAAGAAA CCAGTATCAC AAACACCTGT CAGACCAGCA GCTCTTCCTC CTCAGTTTCT 
AAACACAGAA AGAATGAAAA TAAATCTAAC CCCAAAGGAA TCTGTGAGGA AGAAACCCAC 
AATGCACCAA CAGCCAATGC TCTTCTTGAT ACGAACCATC TAGAAACAGA TATCTGGTCT 
GCAATGCCAA CTCTTTCCAA CGGGAATTCT GAGCCCAGAA GACCTAAAAT CACATTTGAT 
GATGTGCTTC ACAATGCAGA TTATTATATG CAAGAAGCGA AGAAGCTAAA GCATAAAGCG 
GATGCATTGC TGGAGAAGTT CGGCAAAGCA GTGAATTATG CTGATGCTGC CTTGTCCTTC 
ATCGAGTGTG GGAATGCTAT GGAGCGAGAT CCATTAGAAG CTAAATCTCC ATACACCATG 
TACTCAGAGA CTGTGGAACT CATCAGGTAT GCAATGAGAC TCAAGAATTT CACAGGCTCT 
TCTGCCACAG AAGGGGACAA GAAACTAGCA GTTTTATGCT ACCGATGCTT ATCGCTTCTG 
TACTTGAGAA TGTTTAAACT AAAGAAGGAC CATGCTATGA AGTACTCCAG ATCTCTGATG 
GAATATTTTA AGAACTCTTC AAAAGCCTCA CAGGCCCCCT CACCTTGGGG CGGCAATGGA 
AAGAGCACAG AAACACCATC ACCCATGCCC CTGAGCCCAT CCCCTGTCAG CTCTGCTGGA 
GGCAGTGCAG GGATCACTGG CTCCTCCTCA GCAGGGACCA TCACCATTCC TCAGCGCATT 
CACCACATGG CTGCCAGCCA TGTCAACATC ACCAACAATG TTCTGCGAAG CTACGAGCAC 
TGGGACATGG CTGACAAGCT GACTAAGGAG AATAAAGAAT TCTTTGTGGA CCTGGACACA 
GTGATGGGAC CCTTAACACA ACACAGCAGT ATGACCAATC TGGTTCGTTA CGTTCGCCAA 
GGACTCCACT GGCTCCACAT TGAGGCTCAT TTGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025006234.1
CDS	16..4014
Translation	MCMCARARAAAAAVPASPSQAAAARAAGGGRRRRRTTSPGRLRGGRRGRRCPCPCPCRAASARLGAGRGYHFFESPHQIVMDLFDFFRDWDLEQQCHYEQDRNALKRKEWERRNQEVQQDEDLFASGFNLFGEPYKTNKGDALANRVQNTLGNYDEMKDLLTNHSNQSHLVGIPKNSVPQTPIEKNEQNFFSEQRNRVIPSHQISGHSSTSMPPPSSLSSNSALLHSHQSSRKSRTDWSRSGHNCSGTQQSQSSSQQSRTKHSSSHDLPQGRYEDLYNCQSEQHKGGGTEEANSMAASSHSRRHTHSKSAAGEHTYKENSHSKSPTELEFVGHGPGSPLPSTSLLSSNNGLSSQNFPPGLHCKSSMVQQKPTAYVRPMDGQDQVPNDSPELKPPIEIESGYGNQSFGTLLEGKVNTPSSKNKVPKLNIPPVGEEMSHSWPPPLTAIHTPGKAEQTKFSIPSKVQDSQHLTSGYNVQKWSDPGGKVATKSVTQKSMLEDDLKLSSDEDDNEQAAEKTKLRNIPVNGLPVSVAHAAGLAHSSGGSGSSSESESSSESDSDSESSSSDSDCNEESRSATPEPEPPSTNKWQLDKWLNKVNPHNKTIISNQNEPHSETSSQPQNSQQAEGQNKGKLSNSLPPADSKDQAILSPIREKAKPRVAQKTPEAKSSKQKSPAHNEPTAQRTTGKKQPKKVERTSSVEEYNWPKPNNTSNTPKEKDTQEPPEQPKVRTKAAVGKTAPRKEPRTSTGLTSEKKKYRGPSKIVPKSREFIETDSSTSDSNTDQEESLQAKILPACSGPANSIKVKDPGSNILSVSSLTSNGSNTSIDQTSNDLEEQLFSPIPIVQNELLSPLREYEELKSLWVKIDLSLLSRIPGQEPFETISVKTEPREANVKHRRPSRESPTPAAEKTSAKSKRKHKSENLETFVETKKQRLEDPSASCLLPPCISPIPNHRLTSTKESSSNLVKKMAKKREEKLFPPPLSPLLDEPVHRRHSNDNSSFSQETSITNTCQTSSSSSSVSKHRKNENKSNPKGICEEETHNAPTANALLDTNHLETDIWSAMPTLSNGNSEPRRPKITFDDVLHNADYYMQEAKKLKHKADALLEKFGKAVNYADAALSFIECGNAMERDPLEAKSPYTMYSETVELIRYAMRLKNFTGSSATEGDKKLAVLCYRCLSLLYLRMFKLKKDHAMKYSRSLMEYFKNSSKASQAPSPWGGNGKSTETPSPMPLSPSPVSSAGGSAGITGSSSAGTITIPQRIHHMAASHVNITNNVLRSYEHWDMADKLTKENKEFFVDLDTVMGPLTQHSSMTNLVRYVRQGLHWLHIEAHLL

Target ORF information:

RefSeq Version	XM_025150466.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus AF4/FMR2 family member 2 (AFF2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025150466.1

ORF Insert Sequence:

ATGTGTATGT GTGCGCGCGC CAGGGCCGCT GCCGCCGCTG TGCCCGCCTC TCCCAGCCAG 
GCAGCAGCCG CGCGCGCTGC TGGCGGCGGG CGGCGGCGGC GGCGCACCAC GAGCCCCGGG 
CGGCTCCGCG GCGGCCGCCG CGGCCGCCGC TGCCCGTGCC CCTGCCCCTG CCGCGCTGCC 
TCTGCTCGGC TCGGCGCGGG GAGAGGGTAC CACTTCTTCG AGTCTCCGCA CCAGATCGTA 
ATGGACCTAT TCGACTTTTT CAGGGACTGG GACTTAGAGC AGCAGTGTCA CTATGAGCAA 
GACCGGAATG CACTTAAGCG AAAGGAATGG GAGAGAAGAA ACCAAGAAGT CCAGCAAGAT 
GAAGACCTCT TTGCTTCAGG TTTTAATCTC TTTGGGGAAC CCTACAAGAC AAATAAAGGT 
GATGCTCTTG CAAACCGTGT CCAGAACACA TTGGGAAACT ATGATGAGAT GAAGGATCTG 
CTAACTAACC ATTCCAATCA GAGCCATCTT GTAGGAATCC CAAAGAATTC TGTCCCACAG 
ACACCCATCG AGAAAAATGA ACAGAACTTT TTCTCAGAAC AAAGAAACAG GGTGATCCCA 
TCTCATCAGA TCAGTGGCCA CTCATCGACA TCAATGCCTC CACCTTCGTC ACTGTCATCT 
AATTCTGCTT TGCTGCACAG TCACCAGAGC AGCAGGAAGT CGAGAACAGA CTGGTCACGC 
AGTGGTCACA ACTGTAGTGG GACCCAGCAA AGCCAATCCA GTAGTCAGCA AAGTCGGACA 
AAGCATAGCT CTTCTCATGA CCTGCCACAA GGCAGATATG AAGACCTCTA TAATTGTCAG 
AGTGAGCAGC ATAAAGGTGG AGGAACTGAG GAAGCAAATT CTATGGCAGC ATCTTCACAT 
TCAAGGCGGC ATACTCATTC CAAGTCAGCA GCTGGAGAAC ATACTTACAA AGAAAACAGT 
CATTCAAAGT CACCCACGGA GCTTGAGTTT GTAGGTCATG GTCCTGGATC TCCACTTCCT 
TCCACATCTT TGTTGTCTTC AAACAATGGT CTTTCAAGCC AGAATTTCCC ACCAGGACTT 
CACTGTAAAA GCAGCATGGT CCAGCAAAAG CCTACAGCAT ATGTTAGGCC GATGGATGGT 
CAGGACCAGG TACCGAATGA CTCACCTGAA CTGAAACCTC CCATAGAAAT TGAGAGTGGA 
TATGGAAATC AGTCCTTTGG AACACTGTTG GAAGGAAAAG TGAACACACC TAGTTCAAAA 
AATAAAGTAC CAAAGCTTAA TATTCCTCCT GTTGGTGAAG AGATGTCCCA TTCCTGGCCT 
CCTCCTCTCA CTGCTATTCA CACTCCTGGA AAGGCTGAAC AGACCAAGTT TTCTATCCCA 
AGCAAAGTAC AAGACTCCCA ACATCTGACC TCAGGATACA ATGTACAAAA GTGGAGTGAT 
CCAGGAGGGA AAGTTGCAAC TAAGTCAGTG ACACAAAAGT CGATGCTTGA GGATGATCTG 
AAACTCAGCA GTGATGAAGA TGACAATGAA CAGGCTGCTG AAAAGACAAA ATTGAGAAAC 
ATTCCTGTAA ACGGCCTGCC GGTGTCGGTG GCACATGCAG CTGGCTTAGC CCATTCCAGT 
GGTGGCTCTG GGAGCTCCAG TGAGTCCGAG AGCAGCTCTG AGTCTGACTC CGACAGTGAG 
AGCAGCTCCA GTGACAGCGA CTGCAATGAG GAATCACGCA GTGCCACGCC AGAGCCTGAA 
CCTCCCTCAA CAAACAAATG GCAGCTGGAT AAGTGGCTAA ATAAAGTGAA CCCCCACAAC 
AAGACCATCA TCAGCAATCA AAATGAGCCT CACAGTGAGA CCAGCTCCCA GCCACAGAAC 
AGCCAGCAGG CTGAAGGGCA AAACAAAGGG AAGCTCAGCA ACAGCCTGCC CCCAGCTGAT 
TCCAAAGACC AGGCGATCCT CAGTCCCATC CGAGAGAAAG CCAAACCACG GGTTGCCCAG 
AAAACTCCAG AGGCAAAAAG CTCCAAGCAG AAGTCGCCAG CTCATAATGA GCCAACTGCA 
CAAAGGACTA CTGGGAAAAA GCAACCCAAA AAGGTAGAAA GAACTTCCAG TGTAGAAGAG 
TATAACTGGC CCAAACCAAA TAATACCAGC AACACTCCCA AAGAAAAAGA CACACAGGAA 
CCCCCCGAGC AGCCAAAGGT TCGAACTAAA GCAGCAGTTG GCAAGACTGC CCCAAGGAAG 
GAACCTCGAA CAAGCACAGG TCTCACTTCG GAGAAGAAAA AGTACAGAGG CCCCAGCAAA 
ATTGTCCCCA AGTCCCGAGA ATTTATTGAA ACTGATTCAT CTACTTCTGA CTCAAATACA 
GATCAGGAGG AGAGCCTGCA GGCTAAGATA TTGCCAGCTT GTAGTGGACC AGCCAACAGC 
ATCAAAGTGA AGGACCCTGG CAGTAACATC CTCAGTGTAA GTAGCTTAAC TAGCAATGGC 
AGCAACACAT CCATCGACCA GACTAGCAAT GACTTGGAGG AGCAGCTCTT TTCTCCTATC 
CCAATTGTAC AAAATGAACT TCTGTCCCCA CTGAGAGAAT ATGAAGAACT CAAATCTCTT 
TGGGTGAAAA TAGACCTTAG TTTACTCTCT AGAATACCTG GACAGGAGCC TTTTGAGACC 
ATATCTGTGA AAACTGAACC AAGAGAGGCA AATGTGAAAC ACAGACGACC ATCTCGAGAG 
TCCCCTACCC CAGCAGCTGA AAAAACATCT GCAAAATCCA AGAGGAAACA CAAGTCGGAA 
AACCTGGAAA CCTTTGTTGA AACCAAGAAG CAGCGCCTGG AGGACCCTTC AGCTTCGTGC 
TTGCTCCCAC CTTGTATCTC TCCAATACCA AACCACAGAT TAACCAGCAC TAAAGAGAGC 
AGCAGTAACT TGGTGAAGAA GATGGCAAAG AAAAGAGAAG AGAAGCTCTT CCCTCCTCCA 
TTATCCCCCC TGTTGGATGA GCCTGTGCAC CGTCGGCACA GCAATGACAA CAGCTCCTTC 
AGCCAAGAAA CCAGTATCAC AAACACCTGT CAGACCAGCA GCTCTTCCTC CTCAGTTTCT 
AAACACAGAA AGAATGAAAA TAAATCTAAC CCCAAAGGAA TCTGTGAGGA AGAAACCCAC 
AATGCACCAA CAGCCAATGC TCTTCTTGAT ACGAACCATC TAGAAACAGA TATCTGGTCT 
GCAATGCCAA CTCTTTCCAA CGGGAATTCT GAGCCCAGAA GACCTAAAAT CACATTTGAT 
GATGTGCTTC ACAATGCAGA TTATTATATG CAAGAAGCGA AGAAGCTAAA GCATAAAGCG 
GATGCATTGC TGGAGAAGTT CGGCAAAGCA GTGAATTATG CTGATGCTGC CTTGTCCTTC 
ATCGAGTGTG GGAATGCTAT GGAGCGAGAT CCATTAGAAG CTAAATCTCC ATACACCATG 
TACTCAGAGA CTGTGGAACT CATCAGGTAT GCAATGAGAC TCAAGAATTT CACAGGCTCT 
TCTGCCACAG AAGGGGACAA GAAACTAGCA GTTTTATGCT ACCGATGCTT ATCGCTTCTG 
TACTTGAGAA TGTTTAAACT AAAGAAGGAC CATGCTATGA AGTACTCCAG ATCTCTGATG 
GAATATTTTA AGAACTCTTC AAAAGCCTCA CAGGCCCCCT CACCTTGGGG CGGCAATGGA 
AAGAGCACAG AAACACCATC ACCCATGCCC CTGAGCCCAT CCCCTGTCAG CTCTGCTGGA 
GGCAGTGCAG GGATCACTGG CTCCTCCTCA GCAGGGACCA TCACCATTCC TCAGCGCATT 
CACCACATGG CTGCCAGCCA TGTCAACATC ACCAACAATG TTCTGCGAAG CTACGAGCAC 
TGGGACATGG CTGACAAGCT GACTAAGGAG AATAAAGAAT TCTTTGTGGA CCTGGACACA 
GTGATGGGAC CCTTAACACA ACACAGCAGT ATGACCAATC TGGTTCGTTA CGTTCGCCAA 
GGACTCCACT GGCTCCACAT TGAGGCTCAT TTGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF2 ( XM_025150467.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025006235.1 Gallus gallus AF4/FMR2 family member 2 (AFF2), transcript variant X5, mRNA.

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CloneID	OGa150692
Clone ID Related Accession (Same CDS sequence)	XM_025150467.1
Accession Version	XM_025150467.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	AF4/FMR2 family member 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTATGT GTGCGCGCGC CAGGGCCGCT GCCGCCGCTG TGCCCGCCTC TCCCAGCCAG 
GCAGCAGCCG CGCGCGCTGC TGGCGGCGGG CGGCGGCGGC GGCGCACCAC GAGCCCCGGG 
CGGCTCCGCG GCGGCCGCCG CGGCCGCCGC TGCCCGTGCC CCTGCCCCTG CCGCGCTGCC 
TCTGCTCGGC TCGGCGCGGG GAGAGGGTAC CACTTCTTCG AGTCTCCGCA CCAGATCGTA 
ATGGACCTAT TCGACTTTTT CAGGGACTGG GACTTAGAGC AGCAGTGTCA CTATGAGCAA 
GACCGGAATG CACTTAAGCG AAAGGAATGG GAGAGAAGAA ACCAAGAAGT CCAGCAAGAT 
GAAGACCTCT TTGCTTCAGG TTTTAATCTC TTTGGGGAAC CCTACAAGAC AAATAAAGGT 
GATGCTCTTG CAAACCGTGT CCAGAACACA TTGGGAAACT ATGATGAGAT GAAGGATCTG 
CTAACTAACC ATTCCAATCA GAGCCATCTT GTAGGAATCC CAAAGAATTC TGTCCCACAG 
ACACCCATCG AGAAAAATGA ACAGAACTTT TTCTCAGAAC AAAGAAACAG GGTGATCCCA 
TCTCATCAGA TCAGTGGCCA CTCATCGACA TCAATGCCTC CACCTTCGTC ACTGTCATCT 
AATTCTGCTT TGCTGCACAG TCACCAGAGC AGCAGGAAGT CGAGAACAGA CTGGTCACGC 
AGTGGTCACA ACTGTAGTGG GACCCAGCAA AGCCAATCCA GTAGTCAGCA AAGTCGGACA 
AAGCATAGCT CTTCTCATGA CCTGCCACAA GGCAGATATG AAGACCTCTA TAATTGTCAG 
AGTGAGCAGC ATAAAGGTGG AGGAACTGAG GAAGCAAATT CTATGGCAGC ATCTTCACAT 
TCAAGGCGGC ATACTCATTC CAAGTCAGCA GCTGGAGAAC ATACTTACAA AGAAAACAGT 
CATTCAAAGT CACCCACGGA GCTTGAGTTT GTAGGTCATG GTCCTGGATC TCCACTTCCT 
TCCACATCTT TGTTGTCTTC AAACAATGGT CTTTCAAGCC AGAATTTCCC ACCAGGACTT 
CACTGTAAAA GCAGCATGGT CCAGCAAAAG CCTACAGCAT ATGTTAGGCC GATGGATGGT 
CAGGACCAGG TACCGAATGA CTCACCTGAA CTGAAACCTC CCATAGAAAT TGAGAGTGGA 
TATGGAAATC AGTCCTTTGG AACACTGTTG GAAGGAAAAG TGAACACACC TAGTTCAAAA 
AATAAAGTAC CAAAGCTTAA TATTCCTCCT GTTGGTGAAG TTACCCTTCC CAATGACTCC 
AGCAGTGTGG AAGAAATACT GCGTGACTCC CAACATCTGA CCTCAGGATA CAATGTACAA 
AAGTGGAGTG ATCCAGGAGG GAAAGTTGCA ACTAAGTCAG TGACACAAAA GTCGATGCTT 
GAGGATGATC TGAAACTCAG CAGTGATGAA GATGACAATG AACAGGCTGC TGAAAAGACA 
AAATTGAGAA ACATTCCTGT AAACGGCCTG CCGGTGTCGG TGGCACATGC AGCTGGCTTA 
GCCCATTCCA GTGGTGGCTC TGGGAGCTCC AGTGAGTCCG AGAGCAGCTC TGAGTCTGAC 
TCCGACAGTG AGAGCAGCTC CAGTGACAGC GACTGCAATG AGGAATCACG CAGTGCCACG 
CCAGAGCCTG AACCTCCCTC AACAAACAAA TGGCAGCTGG ATAAGTGGCT AAATAAAGTG 
AACCCCCACA ACAAGACCAT CATCAGCAAT CAAAATGAGC CTCACAGTGA GACCAGCTCC 
CAGCCACAGA ACAGCCAGCA GGCTGAAGGG CAAAACAAAG GGAAGCTCAG CAACAGCCTG 
CCCCCAGCTG ATTCCAAAGA CCAGGCGATC CTCAGTCCCA TCCGAGAGAA AGCCAAACCA 
CGGGTTGCCC AGAAAACTCC AGAGGCAAAA AGCTCCAAGC AGAAGTCGCC AGCTCATAAT 
GAGCCAACTG CACAAAGGAC TACTGGGAAA AAGCAACCCA AAAAGGTAGA AAGAACTTCC 
AGTGTAGAAG AGTATAACTG GCCCAAACCA AATAATACCA GCAACACTCC CAAAGAAAAA 
GACACACAGG AACCCCCCGA GCAGCCAAAG GTTCGAACTA AAGCAGCAGT TGGCAAGACT 
GCCCCAAGGA AGGAACCTCG AACAAGCACA GGTCTCACTT CGGAGAAGAA AAAGTACAGA 
GGCCCCAGCA AAATTGTCCC CAAGTCCCGA GAATTTATTG AAACTGATTC ATCTACTTCT 
GACTCAAATA CAGATCAGGA GGAGAGCCTG CAGGCTAAGA TATTGCCAGC TTGTAGTGGA 
CCAGCCAACA GCATCAAAGT GAAGGACCCT GGCAGTAACA TCCTCAGTGT AAGTAGCTTA 
ACTAGCAATG GCAGCAACAC ATCCATCGAC CAGACTAGCA ATGACTTGGA GGAGCAGCTC 
TTTTCTCCTA TCCCAATTGT ACAAAATGAA CTTCTGTCCC CACTGAGAGA ATATGAAGAA 
CTCAAATCTC TTTGGGTGAA AATAGACCTT AGTTTACTCT CTAGAATACC TGGACAGGAG 
CCTTTTGAGA CCATATCTGT GAAAACTGAA CCAAGAGAGG CAAATGTGAA ACACAGACGA 
CCATCTCGAG AGTCCCCTAC CCCAGCAGCT GAAAAAACAT CTGCAAAATC CAAGAGGAAA 
CACAAGTCGG AAAACCTGGA AACCTTTGTT GAAACCAAGA AGCAGCGCCT GGAGGACCCT 
TCAGCTTCGT GCTTGCTCCC ACCTTGTATC TCTCCAATAC CAAACCACAG ATTAACCAGC 
ACTAAAGAGA GCAGCAGTAA CTTGGTGAAG AAGATGGCAA AGAAAAGAGA AGAGAAGCTC 
TTCCCTCCTC CATTATCCCC CCTGTTGGAT GAGCCTGTGC ACCGTCGGCA CAGCAATGAC 
AACAGCTCCT TCAGCCAAGA AACCAGTATC ACAAACACCT GTCAGACCAG CAGCTCTTCC 
TCCTCAGTTT CTAAACACAG AAAGAATGAA AATAAATCTA ACCCCAAAGG AATCTGTGAG 
GAAGAAACCC ACAATGCACC AACAGCCAAT GCTCTTCTTG ATACGAACCA TCTAGAAACA 
GATATCTGGT CTGCAATGCC AACTCTTTCC AACGGGAATT CTGAGCCCAG AAGACCTAAA 
ATCACATTTG ATGATGTGCT TCACAATGCA GATTATTATA TGCAAGAAGC GAAGAAGCTA 
AAGCATAAAG CGGATGCATT GCTGGAGAAG TTCGGCAAAG CAGTGAATTA TGCTGATGCT 
GCCTTGTCCT TCATCGAGTG TGGGAATGCT ATGGAGCGAG ATCCATTAGA AGCTAAATCT 
CCATACACCA TGTACTCAGA GACTGTGGAA CTCATCAGGT ATGCAATGAG ACTCAAGAAT 
TTCACAGGCT CTTCTGCCAC AGAAGGGGAC AAGAAACTAG CAGTTTTATG CTACCGATGC 
TTATCGCTTC TGTACTTGAG AATGTTTAAA CTAAAGAAGG ACCATGCTAT GAAGTACTCC 
AGATCTCTGA TGGAATATTT TAAGAACTCT TCAAAAGCCT CACAGGCCCC CTCACCTTGG 
GGCGGCAATG GAAAGAGCAC AGAAACACCA TCACCCATGC CCCTGAGCCC ATCCCCTGTC 
AGCTCTGCTG GAGGCAGTGC AGGGATCACT GGCTCCTCCT CAGCAGGGAC CATCACCATT 
CCTCAGCGCA TTCACCACAT GGCTGCCAGC CATGTCAACA TCACCAACAA TGTTCTGCGA 
AGCTACGAGC ACTGGGACAT GGCTGACAAG CTGACTAAGG AGAATAAAGA ATTCTTTGTG 
GACCTGGACA CAGTGATGGG ACCCTTAACA CAACACAGCA GTATGACCAA TCTGGTTCGT 
TACGTTCGCC AAGGACTCCA CTGGCTCCAC ATTGAGGCTC ATTTGTTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025006235.1
CDS	16..3966
Translation	MCMCARARAAAAAVPASPSQAAAARAAGGGRRRRRTTSPGRLRGGRRGRRCPCPCPCRAASARLGAGRGYHFFESPHQIVMDLFDFFRDWDLEQQCHYEQDRNALKRKEWERRNQEVQQDEDLFASGFNLFGEPYKTNKGDALANRVQNTLGNYDEMKDLLTNHSNQSHLVGIPKNSVPQTPIEKNEQNFFSEQRNRVIPSHQISGHSSTSMPPPSSLSSNSALLHSHQSSRKSRTDWSRSGHNCSGTQQSQSSSQQSRTKHSSSHDLPQGRYEDLYNCQSEQHKGGGTEEANSMAASSHSRRHTHSKSAAGEHTYKENSHSKSPTELEFVGHGPGSPLPSTSLLSSNNGLSSQNFPPGLHCKSSMVQQKPTAYVRPMDGQDQVPNDSPELKPPIEIESGYGNQSFGTLLEGKVNTPSSKNKVPKLNIPPVGEVTLPNDSSSVEEILRDSQHLTSGYNVQKWSDPGGKVATKSVTQKSMLEDDLKLSSDEDDNEQAAEKTKLRNIPVNGLPVSVAHAAGLAHSSGGSGSSSESESSSESDSDSESSSSDSDCNEESRSATPEPEPPSTNKWQLDKWLNKVNPHNKTIISNQNEPHSETSSQPQNSQQAEGQNKGKLSNSLPPADSKDQAILSPIREKAKPRVAQKTPEAKSSKQKSPAHNEPTAQRTTGKKQPKKVERTSSVEEYNWPKPNNTSNTPKEKDTQEPPEQPKVRTKAAVGKTAPRKEPRTSTGLTSEKKKYRGPSKIVPKSREFIETDSSTSDSNTDQEESLQAKILPACSGPANSIKVKDPGSNILSVSSLTSNGSNTSIDQTSNDLEEQLFSPIPIVQNELLSPLREYEELKSLWVKIDLSLLSRIPGQEPFETISVKTEPREANVKHRRPSRESPTPAAEKTSAKSKRKHKSENLETFVETKKQRLEDPSASCLLPPCISPIPNHRLTSTKESSSNLVKKMAKKREEKLFPPPLSPLLDEPVHRRHSNDNSSFSQETSITNTCQTSSSSSSVSKHRKNENKSNPKGICEEETHNAPTANALLDTNHLETDIWSAMPTLSNGNSEPRRPKITFDDVLHNADYYMQEAKKLKHKADALLEKFGKAVNYADAALSFIECGNAMERDPLEAKSPYTMYSETVELIRYAMRLKNFTGSSATEGDKKLAVLCYRCLSLLYLRMFKLKKDHAMKYSRSLMEYFKNSSKASQAPSPWGGNGKSTETPSPMPLSPSPVSSAGGSAGITGSSSAGTITIPQRIHHMAASHVNITNNVLRSYEHWDMADKLTKENKEFFVDLDTVMGPLTQHSSMTNLVRYVRQGLHWLHIEAHLL

Target ORF information:

RefSeq Version	XM_025150467.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus AF4/FMR2 family member 2 (AFF2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025150467.1

ORF Insert Sequence:

ATGTGTATGT GTGCGCGCGC CAGGGCCGCT GCCGCCGCTG TGCCCGCCTC TCCCAGCCAG 
GCAGCAGCCG CGCGCGCTGC TGGCGGCGGG CGGCGGCGGC GGCGCACCAC GAGCCCCGGG 
CGGCTCCGCG GCGGCCGCCG CGGCCGCCGC TGCCCGTGCC CCTGCCCCTG CCGCGCTGCC 
TCTGCTCGGC TCGGCGCGGG GAGAGGGTAC CACTTCTTCG AGTCTCCGCA CCAGATCGTA 
ATGGACCTAT TCGACTTTTT CAGGGACTGG GACTTAGAGC AGCAGTGTCA CTATGAGCAA 
GACCGGAATG CACTTAAGCG AAAGGAATGG GAGAGAAGAA ACCAAGAAGT CCAGCAAGAT 
GAAGACCTCT TTGCTTCAGG TTTTAATCTC TTTGGGGAAC CCTACAAGAC AAATAAAGGT 
GATGCTCTTG CAAACCGTGT CCAGAACACA TTGGGAAACT ATGATGAGAT GAAGGATCTG 
CTAACTAACC ATTCCAATCA GAGCCATCTT GTAGGAATCC CAAAGAATTC TGTCCCACAG 
ACACCCATCG AGAAAAATGA ACAGAACTTT TTCTCAGAAC AAAGAAACAG GGTGATCCCA 
TCTCATCAGA TCAGTGGCCA CTCATCGACA TCAATGCCTC CACCTTCGTC ACTGTCATCT 
AATTCTGCTT TGCTGCACAG TCACCAGAGC AGCAGGAAGT CGAGAACAGA CTGGTCACGC 
AGTGGTCACA ACTGTAGTGG GACCCAGCAA AGCCAATCCA GTAGTCAGCA AAGTCGGACA 
AAGCATAGCT CTTCTCATGA CCTGCCACAA GGCAGATATG AAGACCTCTA TAATTGTCAG 
AGTGAGCAGC ATAAAGGTGG AGGAACTGAG GAAGCAAATT CTATGGCAGC ATCTTCACAT 
TCAAGGCGGC ATACTCATTC CAAGTCAGCA GCTGGAGAAC ATACTTACAA AGAAAACAGT 
CATTCAAAGT CACCCACGGA GCTTGAGTTT GTAGGTCATG GTCCTGGATC TCCACTTCCT 
TCCACATCTT TGTTGTCTTC AAACAATGGT CTTTCAAGCC AGAATTTCCC ACCAGGACTT 
CACTGTAAAA GCAGCATGGT CCAGCAAAAG CCTACAGCAT ATGTTAGGCC GATGGATGGT 
CAGGACCAGG TACCGAATGA CTCACCTGAA CTGAAACCTC CCATAGAAAT TGAGAGTGGA 
TATGGAAATC AGTCCTTTGG AACACTGTTG GAAGGAAAAG TGAACACACC TAGTTCAAAA 
AATAAAGTAC CAAAGCTTAA TATTCCTCCT GTTGGTGAAG TTACCCTTCC CAATGACTCC 
AGCAGTGTGG AAGAAATACT GCGTGACTCC CAACATCTGA CCTCAGGATA CAATGTACAA 
AAGTGGAGTG ATCCAGGAGG GAAAGTTGCA ACTAAGTCAG TGACACAAAA GTCGATGCTT 
GAGGATGATC TGAAACTCAG CAGTGATGAA GATGACAATG AACAGGCTGC TGAAAAGACA 
AAATTGAGAA ACATTCCTGT AAACGGCCTG CCGGTGTCGG TGGCACATGC AGCTGGCTTA 
GCCCATTCCA GTGGTGGCTC TGGGAGCTCC AGTGAGTCCG AGAGCAGCTC TGAGTCTGAC 
TCCGACAGTG AGAGCAGCTC CAGTGACAGC GACTGCAATG AGGAATCACG CAGTGCCACG 
CCAGAGCCTG AACCTCCCTC AACAAACAAA TGGCAGCTGG ATAAGTGGCT AAATAAAGTG 
AACCCCCACA ACAAGACCAT CATCAGCAAT CAAAATGAGC CTCACAGTGA GACCAGCTCC 
CAGCCACAGA ACAGCCAGCA GGCTGAAGGG CAAAACAAAG GGAAGCTCAG CAACAGCCTG 
CCCCCAGCTG ATTCCAAAGA CCAGGCGATC CTCAGTCCCA TCCGAGAGAA AGCCAAACCA 
CGGGTTGCCC AGAAAACTCC AGAGGCAAAA AGCTCCAAGC AGAAGTCGCC AGCTCATAAT 
GAGCCAACTG CACAAAGGAC TACTGGGAAA AAGCAACCCA AAAAGGTAGA AAGAACTTCC 
AGTGTAGAAG AGTATAACTG GCCCAAACCA AATAATACCA GCAACACTCC CAAAGAAAAA 
GACACACAGG AACCCCCCGA GCAGCCAAAG GTTCGAACTA AAGCAGCAGT TGGCAAGACT 
GCCCCAAGGA AGGAACCTCG AACAAGCACA GGTCTCACTT CGGAGAAGAA AAAGTACAGA 
GGCCCCAGCA AAATTGTCCC CAAGTCCCGA GAATTTATTG AAACTGATTC ATCTACTTCT 
GACTCAAATA CAGATCAGGA GGAGAGCCTG CAGGCTAAGA TATTGCCAGC TTGTAGTGGA 
CCAGCCAACA GCATCAAAGT GAAGGACCCT GGCAGTAACA TCCTCAGTGT AAGTAGCTTA 
ACTAGCAATG GCAGCAACAC ATCCATCGAC CAGACTAGCA ATGACTTGGA GGAGCAGCTC 
TTTTCTCCTA TCCCAATTGT ACAAAATGAA CTTCTGTCCC CACTGAGAGA ATATGAAGAA 
CTCAAATCTC TTTGGGTGAA AATAGACCTT AGTTTACTCT CTAGAATACC TGGACAGGAG 
CCTTTTGAGA CCATATCTGT GAAAACTGAA CCAAGAGAGG CAAATGTGAA ACACAGACGA 
CCATCTCGAG AGTCCCCTAC CCCAGCAGCT GAAAAAACAT CTGCAAAATC CAAGAGGAAA 
CACAAGTCGG AAAACCTGGA AACCTTTGTT GAAACCAAGA AGCAGCGCCT GGAGGACCCT 
TCAGCTTCGT GCTTGCTCCC ACCTTGTATC TCTCCAATAC CAAACCACAG ATTAACCAGC 
ACTAAAGAGA GCAGCAGTAA CTTGGTGAAG AAGATGGCAA AGAAAAGAGA AGAGAAGCTC 
TTCCCTCCTC CATTATCCCC CCTGTTGGAT GAGCCTGTGC ACCGTCGGCA CAGCAATGAC 
AACAGCTCCT TCAGCCAAGA AACCAGTATC ACAAACACCT GTCAGACCAG CAGCTCTTCC 
TCCTCAGTTT CTAAACACAG AAAGAATGAA AATAAATCTA ACCCCAAAGG AATCTGTGAG 
GAAGAAACCC ACAATGCACC AACAGCCAAT GCTCTTCTTG ATACGAACCA TCTAGAAACA 
GATATCTGGT CTGCAATGCC AACTCTTTCC AACGGGAATT CTGAGCCCAG AAGACCTAAA 
ATCACATTTG ATGATGTGCT TCACAATGCA GATTATTATA TGCAAGAAGC GAAGAAGCTA 
AAGCATAAAG CGGATGCATT GCTGGAGAAG TTCGGCAAAG CAGTGAATTA TGCTGATGCT 
GCCTTGTCCT TCATCGAGTG TGGGAATGCT ATGGAGCGAG ATCCATTAGA AGCTAAATCT 
CCATACACCA TGTACTCAGA GACTGTGGAA CTCATCAGGT ATGCAATGAG ACTCAAGAAT 
TTCACAGGCT CTTCTGCCAC AGAAGGGGAC AAGAAACTAG CAGTTTTATG CTACCGATGC 
TTATCGCTTC TGTACTTGAG AATGTTTAAA CTAAAGAAGG ACCATGCTAT GAAGTACTCC 
AGATCTCTGA TGGAATATTT TAAGAACTCT TCAAAAGCCT CACAGGCCCC CTCACCTTGG 
GGCGGCAATG GAAAGAGCAC AGAAACACCA TCACCCATGC CCCTGAGCCC ATCCCCTGTC 
AGCTCTGCTG GAGGCAGTGC AGGGATCACT GGCTCCTCCT CAGCAGGGAC CATCACCATT 
CCTCAGCGCA TTCACCACAT GGCTGCCAGC CATGTCAACA TCACCAACAA TGTTCTGCGA 
AGCTACGAGC ACTGGGACAT GGCTGACAAG CTGACTAAGG AGAATAAAGA ATTCTTTGTG 
GACCTGGACA CAGTGATGGG ACCCTTAACA CAACACAGCA GTATGACCAA TCTGGTTCGT 
TACGTTCGCC AAGGACTCCA CTGGCTCCAC ATTGAGGCTC ATTTGTTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF2 ( XM_025150468.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025006236.1 Gallus gallus AF4/FMR2 family member 2 (AFF2), transcript variant X6, mRNA.

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CloneID	OGa150693
Clone ID Related Accession (Same CDS sequence)	XM_025150468.1
Accession Version	XM_025150468.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	AF4/FMR2 family member 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGATC TGCTAACTAA CCATTCCAAT CAGAGCCATC TTGTAGGAAT CCCAAAGAAT 
TCTGTCCCAC AGACACCCAT CGAGAAAAAT GAACAGAACT TTTTCTCAGA ACAAAGAAAC 
AGGGTGATCC CATCTCATCA GATCAGTGGC CACTCATCGA CATCAATGCC TCCACCTTCG 
TCACTGTCAT CTAATTCTGC TTTGCTGCAC AGTCACCAGA GCAGCAGGAA GTCGAGAACA 
GACTGGTCAC GCAGTGGTCA CAACTGTAGT GGGACCCAGC AAAGCCAATC CAGTAGTCAG 
CAAAGTCGGA CAAAGCATAG CTCTTCTCAT GACCTGCCAC AAGGCAGATA TGAAGACCTC 
TATAATTGTC AGAGTGAGCA GCATAAAGGT GGAGGAACTG AGGAAGCAAA TTCTATGGCA 
GCATCTTCAC ATTCAAGGCG GCATACTCAT TCCAAGTCAG CAGCTGGAGA ACATACTTAC 
AAAGAAAACA GTCATTCAAA GTCACCCACG GAGCTTGAGT TTGTAGGTCA TGGTCCTGGA 
TCTCCACTTC CTTCCACATC TTTGTTGTCT TCAAACAATG GTCTTTCAAG CCAGAATTTC 
CCACCAGGAC TTCACTGTAA AAGCAGCATG GTCCAGCAAA AGCCTACAGC ATATGTTAGG 
CCGATGGATG GTCAGGACCA GGTACCGAAT GACTCACCTG AACTGAAACC TCCCATAGAA 
ATTGAGAGTG GATATGGAAA TCAGTCCTTT GGAACACTGT TGGAAGGAAA AGTGAACACA 
CCTAGTTCAA AAAATAAAGT ACCAAAGCTT AATATTCCTC CTGTTGGTGA AGCAATGGGA 
GTTGTGCATT GGATCAGCAA CAGCGTAAGT GGATCGAGGA GTGCAGCAAG CTGTGGATCG 
AAGGTTACCC TTCCCAATGA CTCCAGCAGT GTGGAAGAAA TACTGCGTGA GATGTCCCAT 
TCCTGGCCTC CTCCTCTCAC TGCTATTCAC ACTCCTGGAA AGGCTGAACA GACCAAGTTT 
TCTATCCCAA GCAAAGTACA AGACTCCCAA CATCTGACCT CAGGATACAA TGTACAAAAG 
TGGAGTGATC CAGGAGGGAA AGTTGCAACT AAGTCAGTGA CACAAAAGTC GATGCTTGAG 
GATGATCTGA AACTCAGCAG TGATGAAGAT GACAATGAAC AGGCTGCTGA AAAGACAAAA 
TTGAGAAACA TTCCTGTAAA CGGCCTGCCG GTGTCGGTGG CACATGCAGC TGGCTTAGCC 
CATTCCAGTG GTGGCTCTGG GAGCTCCAGT GAGTCCGAGA GCAGCTCTGA GTCTGACTCC 
GACAGTGAGA GCAGCTCCAG TGACAGCGAC TGCAATGAGG AATCACGCAG TGCCACGCCA 
GAGCCTGAAC CTCCCTCAAC AAACAAATGG CAGCTGGATA AGTGGCTAAA TAAAGTGAAC 
CCCCACAACA AGACCATCAT CAGCAATCAA AATGAGCCTC ACAGTGAGAC CAGCTCCCAG 
CCACAGAACA GCCAGCAGGC TGAAGGGCAA AACAAAGGGA AGCTCAGCAA CAGCCTGCCC 
CCAGCTGATT CCAAAGACCA GGCGATCCTC AGTCCCATCC GAGAGAAAGC CAAACCACGG 
GTTGCCCAGA AAACTCCAGA GGCAAAAAGC TCCAAGCAGA AGTCGCCAGC TCATAATGAG 
CCAACTGCAC AAAGGACTAC TGGGAAAAAG CAACCCAAAA AGGTAGAAAG AACTTCCAGT 
GTAGAAGAGT ATAACTGGCC CAAACCAAAT AATACCAGCA ACACTCCCAA AGAAAAAGAC 
ACACAGGAAC CCCCCGAGCA GCCAAAGGTT CGAACTAAAG CAGCAGTTGG CAAGACTGCC 
CCAAGGAAGG AACCTCGAAC AAGCACAGGT CTCACTTCGG AGAAGAAAAA GTACAGAGGC 
CCCAGCAAAA TTGTCCCCAA GTCCCGAGAA TTTATTGAAA CTGATTCATC TACTTCTGAC 
TCAAATACAG ATCAGGAGGA GAGCCTGCAG GCTAAGATAT TGCCAGCTTG TAGTGGACCA 
GCCAACAGCA TCAAAGTGAA GGACCCTGGC AGTAACATCC TCAGTGTAAG TAGCTTAACT 
AGCAATGGCA GCAACACATC CATCGACCAG ACTAGCAATG ACTTGGAGGA GCAGCTCTTT 
TCTCCTATCC CAATTGTACA AAATGAACTT CTGTCCCCAC TGAGAGAATA TGAAGAACTC 
AAATCTCTTT GGGTGAAAAT AGACCTTAGT TTACTCTCTA GAATACCTGG ACAGGAGCCT 
TTTGAGACCA TATCTGTGAA AACTGAACCA AGAGAGGCAA ATGTGAAACA CAGACGACCA 
TCTCGAGAGT CCCCTACCCC AGCAGCTGAA AAAACATCTG CAAAATCCAA GAGGAAACAC 
AAGTCGGAAA ACCTGGAAAC CTTTGTTGAA ACCAAGAAGC AGCGCCTGGA GGACCCTTCA 
GCTTCGTGCT TGCTCCCACC TTGTATCTCT CCAATACCAA ACCACAGATT AACCAGCACT 
AAAGAGAGCA GCAGTAACTT GGTGAAGAAG ATGGCAAAGA AAAGAGAAGA GAAGCTCTTC 
CCTCCTCCAT TATCCCCCCT GTTGGATGAG CCTGTGCACC GTCGGCACAG CAATGACAAC 
AGCTCCTTCA GCCAAGAAAC CAGTATCACA AACACCTGTC AGACCAGCAG CTCTTCCTCC 
TCAGTTTCTA AACACAGAAA GAATGAAAAT AAATCTAACC CCAAAGGAAT CTGTGAGGAA 
GAAACCCACA ATGCACCAAC AGCCAATGCT CTTCTTGATA CGAACCATCT AGAAACAGAT 
ATCTGGTCTG CAATGCCAAC TCTTTCCAAC GGGAATTCTG AGCCCAGAAG ACCTAAAATC 
ACATTTGATG ATGTGCTTCA CAATGCAGAT TATTATATGC AAGAAGCGAA GAAGCTAAAG 
CATAAAGCGG ATGCATTGCT GGAGAAGTTC GGCAAAGCAG TGAATTATGC TGATGCTGCC 
TTGTCCTTCA TCGAGTGTGG GAATGCTATG GAGCGAGATC CATTAGAAGC TAAATCTCCA 
TACACCATGT ACTCAGAGAC TGTGGAACTC ATCAGGTATG CAATGAGACT CAAGAATTTC 
ACAGGCTCTT CTGCCACAGA AGGGGACAAG AAACTAGCAG TTTTATGCTA CCGATGCTTA 
TCGCTTCTGT ACTTGAGAAT GTTTAAACTA AAGAAGGACC ATGCTATGAA GTACTCCAGA 
TCTCTGATGG AATATTTTAA GAACTCTTCA AAAGCCTCAC AGGCCCCCTC ACCTTGGGGC 
GGCAATGGAA AGAGCACAGA AACACCATCA CCCATGCCCC TGAGCCCATC CCCTGTCAGC 
TCTGCTGGAG GCAGTGCAGG GATCACTGGC TCCTCCTCAG CAGGGACCAT CACCATTCCT 
CAGCGCATTC ACCACATGGC TGCCAGCCAT GTCAACATCA CCAACAATGT TCTGCGAAGC 
TACGAGCACT GGGACATGGC TGACAAGCTG ACTAAGGAGA ATAAAGAATT CTTTGTGGAC 
CTGGACACAG TGATGGGACC CTTAACACAA CACAGCAGTA TGACCAATCT GGTTCGTTAC 
GTTCGCCAAG GACTCCACTG GCTCCACATT GAGGCTCATT TGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025006236.1
CDS	388..4035
Translation	MKDLLTNHSNQSHLVGIPKNSVPQTPIEKNEQNFFSEQRNRVIPSHQISGHSSTSMPPPSSLSSNSALLHSHQSSRKSRTDWSRSGHNCSGTQQSQSSSQQSRTKHSSSHDLPQGRYEDLYNCQSEQHKGGGTEEANSMAASSHSRRHTHSKSAAGEHTYKENSHSKSPTELEFVGHGPGSPLPSTSLLSSNNGLSSQNFPPGLHCKSSMVQQKPTAYVRPMDGQDQVPNDSPELKPPIEIESGYGNQSFGTLLEGKVNTPSSKNKVPKLNIPPVGEAMGVVHWISNSVSGSRSAASCGSKVTLPNDSSSVEEILREMSHSWPPPLTAIHTPGKAEQTKFSIPSKVQDSQHLTSGYNVQKWSDPGGKVATKSVTQKSMLEDDLKLSSDEDDNEQAAEKTKLRNIPVNGLPVSVAHAAGLAHSSGGSGSSSESESSSESDSDSESSSSDSDCNEESRSATPEPEPPSTNKWQLDKWLNKVNPHNKTIISNQNEPHSETSSQPQNSQQAEGQNKGKLSNSLPPADSKDQAILSPIREKAKPRVAQKTPEAKSSKQKSPAHNEPTAQRTTGKKQPKKVERTSSVEEYNWPKPNNTSNTPKEKDTQEPPEQPKVRTKAAVGKTAPRKEPRTSTGLTSEKKKYRGPSKIVPKSREFIETDSSTSDSNTDQEESLQAKILPACSGPANSIKVKDPGSNILSVSSLTSNGSNTSIDQTSNDLEEQLFSPIPIVQNELLSPLREYEELKSLWVKIDLSLLSRIPGQEPFETISVKTEPREANVKHRRPSRESPTPAAEKTSAKSKRKHKSENLETFVETKKQRLEDPSASCLLPPCISPIPNHRLTSTKESSSNLVKKMAKKREEKLFPPPLSPLLDEPVHRRHSNDNSSFSQETSITNTCQTSSSSSSVSKHRKNENKSNPKGICEEETHNAPTANALLDTNHLETDIWSAMPTLSNGNSEPRRPKITFDDVLHNADYYMQEAKKLKHKADALLEKFGKAVNYADAALSFIECGNAMERDPLEAKSPYTMYSETVELIRYAMRLKNFTGSSATEGDKKLAVLCYRCLSLLYLRMFKLKKDHAMKYSRSLMEYFKNSSKASQAPSPWGGNGKSTETPSPMPLSPSPVSSAGGSAGITGSSSAGTITIPQRIHHMAASHVNITNNVLRSYEHWDMADKLTKENKEFFVDLDTVMGPLTQHSSMTNLVRYVRQGLHWLHIEAHLL

Target ORF information:

RefSeq Version	XM_025150468.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus AF4/FMR2 family member 2 (AFF2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025150468.1

ORF Insert Sequence:

ATGAAGGATC TGCTAACTAA CCATTCCAAT CAGAGCCATC TTGTAGGAAT CCCAAAGAAT 
TCTGTCCCAC AGACACCCAT CGAGAAAAAT GAACAGAACT TTTTCTCAGA ACAAAGAAAC 
AGGGTGATCC CATCTCATCA GATCAGTGGC CACTCATCGA CATCAATGCC TCCACCTTCG 
TCACTGTCAT CTAATTCTGC TTTGCTGCAC AGTCACCAGA GCAGCAGGAA GTCGAGAACA 
GACTGGTCAC GCAGTGGTCA CAACTGTAGT GGGACCCAGC AAAGCCAATC CAGTAGTCAG 
CAAAGTCGGA CAAAGCATAG CTCTTCTCAT GACCTGCCAC AAGGCAGATA TGAAGACCTC 
TATAATTGTC AGAGTGAGCA GCATAAAGGT GGAGGAACTG AGGAAGCAAA TTCTATGGCA 
GCATCTTCAC ATTCAAGGCG GCATACTCAT TCCAAGTCAG CAGCTGGAGA ACATACTTAC 
AAAGAAAACA GTCATTCAAA GTCACCCACG GAGCTTGAGT TTGTAGGTCA TGGTCCTGGA 
TCTCCACTTC CTTCCACATC TTTGTTGTCT TCAAACAATG GTCTTTCAAG CCAGAATTTC 
CCACCAGGAC TTCACTGTAA AAGCAGCATG GTCCAGCAAA AGCCTACAGC ATATGTTAGG 
CCGATGGATG GTCAGGACCA GGTACCGAAT GACTCACCTG AACTGAAACC TCCCATAGAA 
ATTGAGAGTG GATATGGAAA TCAGTCCTTT GGAACACTGT TGGAAGGAAA AGTGAACACA 
CCTAGTTCAA AAAATAAAGT ACCAAAGCTT AATATTCCTC CTGTTGGTGA AGCAATGGGA 
GTTGTGCATT GGATCAGCAA CAGCGTAAGT GGATCGAGGA GTGCAGCAAG CTGTGGATCG 
AAGGTTACCC TTCCCAATGA CTCCAGCAGT GTGGAAGAAA TACTGCGTGA GATGTCCCAT 
TCCTGGCCTC CTCCTCTCAC TGCTATTCAC ACTCCTGGAA AGGCTGAACA GACCAAGTTT 
TCTATCCCAA GCAAAGTACA AGACTCCCAA CATCTGACCT CAGGATACAA TGTACAAAAG 
TGGAGTGATC CAGGAGGGAA AGTTGCAACT AAGTCAGTGA CACAAAAGTC GATGCTTGAG 
GATGATCTGA AACTCAGCAG TGATGAAGAT GACAATGAAC AGGCTGCTGA AAAGACAAAA 
TTGAGAAACA TTCCTGTAAA CGGCCTGCCG GTGTCGGTGG CACATGCAGC TGGCTTAGCC 
CATTCCAGTG GTGGCTCTGG GAGCTCCAGT GAGTCCGAGA GCAGCTCTGA GTCTGACTCC 
GACAGTGAGA GCAGCTCCAG TGACAGCGAC TGCAATGAGG AATCACGCAG TGCCACGCCA 
GAGCCTGAAC CTCCCTCAAC AAACAAATGG CAGCTGGATA AGTGGCTAAA TAAAGTGAAC 
CCCCACAACA AGACCATCAT CAGCAATCAA AATGAGCCTC ACAGTGAGAC CAGCTCCCAG 
CCACAGAACA GCCAGCAGGC TGAAGGGCAA AACAAAGGGA AGCTCAGCAA CAGCCTGCCC 
CCAGCTGATT CCAAAGACCA GGCGATCCTC AGTCCCATCC GAGAGAAAGC CAAACCACGG 
GTTGCCCAGA AAACTCCAGA GGCAAAAAGC TCCAAGCAGA AGTCGCCAGC TCATAATGAG 
CCAACTGCAC AAAGGACTAC TGGGAAAAAG CAACCCAAAA AGGTAGAAAG AACTTCCAGT 
GTAGAAGAGT ATAACTGGCC CAAACCAAAT AATACCAGCA ACACTCCCAA AGAAAAAGAC 
ACACAGGAAC CCCCCGAGCA GCCAAAGGTT CGAACTAAAG CAGCAGTTGG CAAGACTGCC 
CCAAGGAAGG AACCTCGAAC AAGCACAGGT CTCACTTCGG AGAAGAAAAA GTACAGAGGC 
CCCAGCAAAA TTGTCCCCAA GTCCCGAGAA TTTATTGAAA CTGATTCATC TACTTCTGAC 
TCAAATACAG ATCAGGAGGA GAGCCTGCAG GCTAAGATAT TGCCAGCTTG TAGTGGACCA 
GCCAACAGCA TCAAAGTGAA GGACCCTGGC AGTAACATCC TCAGTGTAAG TAGCTTAACT 
AGCAATGGCA GCAACACATC CATCGACCAG ACTAGCAATG ACTTGGAGGA GCAGCTCTTT 
TCTCCTATCC CAATTGTACA AAATGAACTT CTGTCCCCAC TGAGAGAATA TGAAGAACTC 
AAATCTCTTT GGGTGAAAAT AGACCTTAGT TTACTCTCTA GAATACCTGG ACAGGAGCCT 
TTTGAGACCA TATCTGTGAA AACTGAACCA AGAGAGGCAA ATGTGAAACA CAGACGACCA 
TCTCGAGAGT CCCCTACCCC AGCAGCTGAA AAAACATCTG CAAAATCCAA GAGGAAACAC 
AAGTCGGAAA ACCTGGAAAC CTTTGTTGAA ACCAAGAAGC AGCGCCTGGA GGACCCTTCA 
GCTTCGTGCT TGCTCCCACC TTGTATCTCT CCAATACCAA ACCACAGATT AACCAGCACT 
AAAGAGAGCA GCAGTAACTT GGTGAAGAAG ATGGCAAAGA AAAGAGAAGA GAAGCTCTTC 
CCTCCTCCAT TATCCCCCCT GTTGGATGAG CCTGTGCACC GTCGGCACAG CAATGACAAC 
AGCTCCTTCA GCCAAGAAAC CAGTATCACA AACACCTGTC AGACCAGCAG CTCTTCCTCC 
TCAGTTTCTA AACACAGAAA GAATGAAAAT AAATCTAACC CCAAAGGAAT CTGTGAGGAA 
GAAACCCACA ATGCACCAAC AGCCAATGCT CTTCTTGATA CGAACCATCT AGAAACAGAT 
ATCTGGTCTG CAATGCCAAC TCTTTCCAAC GGGAATTCTG AGCCCAGAAG ACCTAAAATC 
ACATTTGATG ATGTGCTTCA CAATGCAGAT TATTATATGC AAGAAGCGAA GAAGCTAAAG 
CATAAAGCGG ATGCATTGCT GGAGAAGTTC GGCAAAGCAG TGAATTATGC TGATGCTGCC 
TTGTCCTTCA TCGAGTGTGG GAATGCTATG GAGCGAGATC CATTAGAAGC TAAATCTCCA 
TACACCATGT ACTCAGAGAC TGTGGAACTC ATCAGGTATG CAATGAGACT CAAGAATTTC 
ACAGGCTCTT CTGCCACAGA AGGGGACAAG AAACTAGCAG TTTTATGCTA CCGATGCTTA 
TCGCTTCTGT ACTTGAGAAT GTTTAAACTA AAGAAGGACC ATGCTATGAA GTACTCCAGA 
TCTCTGATGG AATATTTTAA GAACTCTTCA AAAGCCTCAC AGGCCCCCTC ACCTTGGGGC 
GGCAATGGAA AGAGCACAGA AACACCATCA CCCATGCCCC TGAGCCCATC CCCTGTCAGC 
TCTGCTGGAG GCAGTGCAGG GATCACTGGC TCCTCCTCAG CAGGGACCAT CACCATTCCT 
CAGCGCATTC ACCACATGGC TGCCAGCCAT GTCAACATCA CCAACAATGT TCTGCGAAGC 
TACGAGCACT GGGACATGGC TGACAAGCTG ACTAAGGAGA ATAAAGAATT CTTTGTGGAC 
CTGGACACAG TGATGGGACC CTTAACACAA CACAGCAGTA TGACCAATCT GGTTCGTTAC 
GTTCGCCAAG GACTCCACTG GCTCCACATT GAGGCTCATT TGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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AFF2 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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