ZNF521 cDNA ORF clone, Gallus gallus(chicken)

The following ZNF521 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZNF521 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa146358 XM_004939803.3
Latest version!
Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa146359 XM_025147905.1
Latest version!
Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa32510 XM_004939804.3
Latest version!
Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa32511 XM_015277987.2
Latest version!
Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa32512 XM_015277988.2
Latest version!
Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa32513 XM_015277989.2
Latest version!
Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa32514 XM_015277990.2
Latest version!
Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa32515 XM_015277991.2
Latest version!
Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa32516 XM_004939803.2
Latest version!
Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa32517 XM_015277992.2
Latest version!
Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
View Old Accession Versions >>
OGa32510 XM_004939804.2 Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa32513 XM_015277989.1 Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa32512 XM_015277988.1 Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa32514 XM_015277990.1 Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa32511 XM_015277987.1 Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa32515 XM_015277991.1 Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa32517 XM_015277992.1 Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa146358
    Clone ID Related Accession (Same CDS sequence) XM_004939803.3
    Accession Version XM_004939803.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product zinc finger protein 521 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004939803.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCTTTGG GGGCCTTGCT TCCATTTTCC ATTATTATGT GGACTACCGG AGCGACAGCG 
    CAGTCCAAGA CCTTGCAGCT GTTTGCTTTT AGGATGTCTC GCCGCAAGCA AGCTAAACCG
    AGATCACTCA AAGAAACCCA GATTCTAGAA ACAACCAATT GTATGAAGCC TTGGGGTTGG
    CGGAGGAGGA AAAGAGTAGA AGAAAATGAA ACAGAGGACC TGCAGGCAGG TGTGGGACAC
    ACAGCCGCGC AGACTGACCC TAACTGTAAA CTAGAAGACA AGACAGAAGA TGGAGAAGTG
    TTAGACTGTA AGAAAAGGCC GGATGAAGGG GAGGAGTTGG AAGAAGAAGC CGTGCACAGC
    TGTGACAGCT GCCTCCAGGT GTTTGAGTCG CTGAGTGATA TCACAGAACA CAAGATTAAT
    CAATGTCAAC TGACAGATGG AGTGGATGTT GAGGATGACC CCACTTGCTC ATGGCCAGCA
    TCATCGCCTT CCAGCAAGGA CCAGACTTCC CCAAGCCATG GAGAAGGTTG TGATTTTGGT
    GAGGAGGAAG GAGGCCCAGG GTTGCCCTAT CAGTGCCAGT TTTGTGACAA ATCGTTCAGC
    CGCCTCAGCT ACTTAAAGCA CCACGAGCAA AGTCACAGTG ACAAACTCCC TTTCAAATGC
    ACTTATTGCA GTCGTCTCTT CAAACACAAA CGAAGCAGGG ACCGCCACAT AAAGCTTCAT
    ACGGGAGACA AGAAGTATCA CTGCAGTGAG TGTGATGCAG CATTCTCAAG GAGCGATCAT
    CTTAAAATTC ACTTAAAGAC TCATACGTCC AACAAGCCAT ATAAATGTGC CATTTGTAGA
    CGTGGATTCC TGTCATCCAG TTCGCTGCAC GGCCACATGC AGGTTCACGA GAGGAACAAG
    GATGGCTCTC AGTCTGCTTC CCGGATGGAG GACTGGAAAA TGAAGGACAC TCAGAAGTGC
    AGTCAGTGTG AAGAAGGCTT TGATTTTCCT GAAGATCTTC AGAAGCACAT TGCGGAATGT
    CACCCTGAGT GTTCTCCTAA TGAAGACCGA TCTGCTCTCC AGTGTGTTTA CTGTCACGAA
    CTCTTTGTAG AGGAAACGTC TCTGGTGAAT CACATGGAGC AGGCTCATAA TGGTGAGAAG
    AAGAATTCAT GCAGCATTTG CTCTGAGAAC TTTCACACTG TGGAAGAGCT GTACAGCCAC
    ATGGACAGTC ACCAGCAGCC AGAATCATGC AACCACAGCA ACAGCCCATC ATTGGTAACT
    GTTGGCTACA CCTCAGTCTC AAGTACCACT CCAGATTCCA ATCTCTCTGT GGATAGTTCA
    ACAATGGTGG AAACGGCTCC CCCTATACCA AAAGGCCGTG GAAGAAAGCG GGCAGCTCAG
    CAAGTGCCAG ATATCACTGG TCCTTCAAGT AAACAGGCAA AAGTTACCTA TAGCTGCATT
    TATTGCAACA AACAGTTATT TTCCAGCCTT GCAGTTTTGC AGATACACCT GAAAACTATG
    CATTTAGATA AACCTGAACA AGCCCATATC TGTCAGTATT GTTTGGAGGT ATTGCCATCT
    CTCTACAATC TGAACGAACA TCTTAAGCAA GTCCACGAAG CTCCGGATCC AGGGCTGATT
    GTTTCCACTA TGCCTGCCAT GGTGTACCAG TGCAACTTCT GCTCTGAAGT GTTCAATGAC
    CTCAACACGC TTCAAGAACA TATCCGATGT TCTCATGGAT TTGCCAACCC TGCTGCTAAG
    GACAGTAATG CGTTCTTTTG CCCCCATTGC TACATGGGGT TTCTTACAGA TTCTTCTCTG
    GAAGAGCATA TTAGACAAGT CCATTGTGAT CTTAGCAGTT CCCGTTTTGG TTCCCCTGTG
    CTCGGAACCC CAAAAGATCC AGTAGTGGAA GTGTATTCTT GTTCTTACTG TACAAATTCT
    CCAATATTTA ATAGCGTTCT TAAACTGAAC AAGCATATCA AGGAGAACCA TAAGAACATT
    CCTTTGGCAC TGAATTACAT CCACAATGGA AAAAAATCCA GAGCCATGAG TCCCTTATCT
    CCTGTAACGA TTGAGCAGAC CTCTTTAAAA ATGATGCAGG CGGTTGGAGG TGCTCCTCCT
    CGTCCTGCAG GTGAATATAT TTGTAATCAA TGTGGTGCTA AGTATACTTC TTTGGACGGT
    TTTCAGACTC ATTTAAAAAC ACATCTTGAT ACTGTCCTGC CAAAACTGAC CTGCCCTCAG
    TGCAACAAGG AGTTTCCAAA TCAGGAGTCT CTGCTGAAGC ATGTTACCAT TCATTTTATG
    ATCACTTCAA CCTACTACAT CTGTGAAAGC TGTGACAAGC AGTTTACTTC TGTGGATGAC
    TTGCAGAAGC ACCTACTAGA CATGCATACA TTTGTATTCT TCCGCTGCAC CTTGTGCCAA
    GAGGTTTTTG ACTCCAAAGT CTCCATCCAA CTACACTTGG CTGTGAAGCA CAGCAATGAA
    AAGAAGGTAT ATAGGTGTAC GTCTTGCAAC TGGGATTTCC GCAATGAGAC TGACCTACAG
    CTTCACGTTA AACACAACCA CTTGGAGAAC CAAGGCAAAG TGCACAAGTG CATCTTCTGT
    GGTGAGTCCT TTGGTACAGA GGTGGAGCTG CAGTGCCATA TTACTACGCA CAGCAAGAAG
    TACAACTGTA AGTTCTGCAG CAAAGCTTTC CATGCCATCA TCCTGCTGGA AAAGCATTTA
    AGGGAAAAAC ATTGTGTTTT TGAAACAAAA ACTCCAAATT GTGGAACGAA TGGGGCATCT
    GAACAGGTTC AGAAGGAAGA AGTGGAGCTC CAGACCTTGT TGACAAATAG CCAGGAGTCC
    CATAACAGCC ATGATGGCAG CGAAGAGGAT GTGGACACAT CTGAGCCTAT GTATGGCTGT
    GACATTTGTG GAGCGGCTTA CACCATGGAG ACTCTCCTGC AAAACCACCA GCTTCGAGAC
    CACAACATTC GACCTGGAGA AAGTGCCATA GTGAAGAAAA AGGCTGAACT CATTAAAGGG
    AATTACAAGT GTAATGTGTG TTCTCGAACA TTCTTCTCTG AAAATGGGCT CCGAGAACAC
    ATGCAGACAC ACTTGGGACC AGTGAAACAT TACATGTGCC CAATCTGTGG CGAGCGGTTT
    CCATCTCTTC TGACTCTTAC TGAACATAAA GTCACACATA GTAAGAGCCT GGACACCGGA
    AACTGCAGGA TTTGCAAGAT GCCTCTCCAG AGTGAGGAGG AATTTTTAGA GCATTGCCAG
    ATGCACCCTG ACTTGAGGAA CTCTTTGACA GGGTTCCGCT GTGTGGTGTG CATGCAGACT
    GTGACCTCCA CGTTGGAACT GAAAATCCAC GGCACATTCC ACATGCAGAA AACTGGAAAT
    GGTTCTGCCG TGCAGTCCAC GGGGCGCGCA CAGCATCTCC AGAAACTGTA CAAGTGTGCT
    TCTTGCCTGA AGGAATTTCG TTCAAAACAG GATTTAGTGA AACTTGACAT CAATGGTCTG
    CCATATGGTC TGTGTGCTAG CTGTGTTAAT CTCAGTAAAA GTGGTAGTCC AAGTGTCAAC
    ATCCCATCAA GCAGTAACAG ACAAGGCATG GGCCAAAATG AGAACTTGAG TTCCATTGAG
    AACAAAAGCA AGGCAGGGGG ACTGAAGACT CGATGTTCTA GTTGCAATGT TAAATTTGAA
    TCAGAAAGTG AACTCCAAAA CCATATTCAG TCAATCCACA GAGAGCTTGT TCCAGACAGC
    AACAGTACAC AGCTGAAAAC ACCACAAGTA TCTCCAATGC CCAGAATCAG CCCCTCCCAA
    ACTGAAGAGA AGAAGACCTA CCAGTGCATC AAATGTCAGA TGGTTTTCTA CAATGAATGG
    GATATCCAAG TTCATGTTGC AAACCACATG ATTGATGAAG GACTGAACCA TGAATGCAAG
    CTCTGCAATC AGACATTTGA CTCTCCTGCC AAACTTCAGT GCCACCTGAT AGAGCACAGC
    TTTGAAGGCA TGGGGGGCAC CTTCAAATGT CCAGTGTGCT TTACAGTGTT TGTCCAAGCA
    AACAAGTTGC AGCAGCACAT TTTCTCAGCC CATGGACAAG AGGACAAGAT CTATGACTGC
    ACACAGTGTC CACAGAAGTT CTTCTTCCAA ACAGAGCTGC AGAATCATAC GATGACCCAG
    CATAGCAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004939860.3
    CDS47..4198
    Translation

    Target ORF information:

    RefSeq Version XM_004939803.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004939803.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCTTTGG GGGCCTTGCT TCCATTTTCC ATTATTATGT GGACTACCGG AGCGACAGCG 
    CAGTCCAAGA CCTTGCAGCT GTTTGCTTTT AGGATGTCTC GCCGCAAGCA AGCTAAACCG
    AGATCACTCA AAGAAACCCA GATTCTAGAA ACAACCAATT GTATGAAGCC TTGGGGTTGG
    CGGAGGAGGA AAAGAGTAGA AGAAAATGAA ACAGAGGACC TGCAGGCAGG TGTGGGACAC
    ACAGCCGCGC AGACTGACCC TAACTGTAAA CTAGAAGACA AGACAGAAGA TGGAGAAGTG
    TTAGACTGTA AGAAAAGGCC GGATGAAGGG GAGGAGTTGG AAGAAGAAGC CGTGCACAGC
    TGTGACAGCT GCCTCCAGGT GTTTGAGTCG CTGAGTGATA TCACAGAACA CAAGATTAAT
    CAATGTCAAC TGACAGATGG AGTGGATGTT GAGGATGACC CCACTTGCTC ATGGCCAGCA
    TCATCGCCTT CCAGCAAGGA CCAGACTTCC CCAAGCCATG GAGAAGGTTG TGATTTTGGT
    GAGGAGGAAG GAGGCCCAGG GTTGCCCTAT CAGTGCCAGT TTTGTGACAA ATCGTTCAGC
    CGCCTCAGCT ACTTAAAGCA CCACGAGCAA AGTCACAGTG ACAAACTCCC TTTCAAATGC
    ACTTATTGCA GTCGTCTCTT CAAACACAAA CGAAGCAGGG ACCGCCACAT AAAGCTTCAT
    ACGGGAGACA AGAAGTATCA CTGCAGTGAG TGTGATGCAG CATTCTCAAG GAGCGATCAT
    CTTAAAATTC ACTTAAAGAC TCATACGTCC AACAAGCCAT ATAAATGTGC CATTTGTAGA
    CGTGGATTCC TGTCATCCAG TTCGCTGCAC GGCCACATGC AGGTTCACGA GAGGAACAAG
    GATGGCTCTC AGTCTGCTTC CCGGATGGAG GACTGGAAAA TGAAGGACAC TCAGAAGTGC
    AGTCAGTGTG AAGAAGGCTT TGATTTTCCT GAAGATCTTC AGAAGCACAT TGCGGAATGT
    CACCCTGAGT GTTCTCCTAA TGAAGACCGA TCTGCTCTCC AGTGTGTTTA CTGTCACGAA
    CTCTTTGTAG AGGAAACGTC TCTGGTGAAT CACATGGAGC AGGCTCATAA TGGTGAGAAG
    AAGAATTCAT GCAGCATTTG CTCTGAGAAC TTTCACACTG TGGAAGAGCT GTACAGCCAC
    ATGGACAGTC ACCAGCAGCC AGAATCATGC AACCACAGCA ACAGCCCATC ATTGGTAACT
    GTTGGCTACA CCTCAGTCTC AAGTACCACT CCAGATTCCA ATCTCTCTGT GGATAGTTCA
    ACAATGGTGG AAACGGCTCC CCCTATACCA AAAGGCCGTG GAAGAAAGCG GGCAGCTCAG
    CAAGTGCCAG ATATCACTGG TCCTTCAAGT AAACAGGCAA AAGTTACCTA TAGCTGCATT
    TATTGCAACA AACAGTTATT TTCCAGCCTT GCAGTTTTGC AGATACACCT GAAAACTATG
    CATTTAGATA AACCTGAACA AGCCCATATC TGTCAGTATT GTTTGGAGGT ATTGCCATCT
    CTCTACAATC TGAACGAACA TCTTAAGCAA GTCCACGAAG CTCCGGATCC AGGGCTGATT
    GTTTCCACTA TGCCTGCCAT GGTGTACCAG TGCAACTTCT GCTCTGAAGT GTTCAATGAC
    CTCAACACGC TTCAAGAACA TATCCGATGT TCTCATGGAT TTGCCAACCC TGCTGCTAAG
    GACAGTAATG CGTTCTTTTG CCCCCATTGC TACATGGGGT TTCTTACAGA TTCTTCTCTG
    GAAGAGCATA TTAGACAAGT CCATTGTGAT CTTAGCAGTT CCCGTTTTGG TTCCCCTGTG
    CTCGGAACCC CAAAAGATCC AGTAGTGGAA GTGTATTCTT GTTCTTACTG TACAAATTCT
    CCAATATTTA ATAGCGTTCT TAAACTGAAC AAGCATATCA AGGAGAACCA TAAGAACATT
    CCTTTGGCAC TGAATTACAT CCACAATGGA AAAAAATCCA GAGCCATGAG TCCCTTATCT
    CCTGTAACGA TTGAGCAGAC CTCTTTAAAA ATGATGCAGG CGGTTGGAGG TGCTCCTCCT
    CGTCCTGCAG GTGAATATAT TTGTAATCAA TGTGGTGCTA AGTATACTTC TTTGGACGGT
    TTTCAGACTC ATTTAAAAAC ACATCTTGAT ACTGTCCTGC CAAAACTGAC CTGCCCTCAG
    TGCAACAAGG AGTTTCCAAA TCAGGAGTCT CTGCTGAAGC ATGTTACCAT TCATTTTATG
    ATCACTTCAA CCTACTACAT CTGTGAAAGC TGTGACAAGC AGTTTACTTC TGTGGATGAC
    TTGCAGAAGC ACCTACTAGA CATGCATACA TTTGTATTCT TCCGCTGCAC CTTGTGCCAA
    GAGGTTTTTG ACTCCAAAGT CTCCATCCAA CTACACTTGG CTGTGAAGCA CAGCAATGAA
    AAGAAGGTAT ATAGGTGTAC GTCTTGCAAC TGGGATTTCC GCAATGAGAC TGACCTACAG
    CTTCACGTTA AACACAACCA CTTGGAGAAC CAAGGCAAAG TGCACAAGTG CATCTTCTGT
    GGTGAGTCCT TTGGTACAGA GGTGGAGCTG CAGTGCCATA TTACTACGCA CAGCAAGAAG
    TACAACTGTA AGTTCTGCAG CAAAGCTTTC CATGCCATCA TCCTGCTGGA AAAGCATTTA
    AGGGAAAAAC ATTGTGTTTT TGAAACAAAA ACTCCAAATT GTGGAACGAA TGGGGCATCT
    GAACAGGTTC AGAAGGAAGA AGTGGAGCTC CAGACCTTGT TGACAAATAG CCAGGAGTCC
    CATAACAGCC ATGATGGCAG CGAAGAGGAT GTGGACACAT CTGAGCCTAT GTATGGCTGT
    GACATTTGTG GAGCGGCTTA CACCATGGAG ACTCTCCTGC AAAACCACCA GCTTCGAGAC
    CACAACATTC GACCTGGAGA AAGTGCCATA GTGAAGAAAA AGGCTGAACT CATTAAAGGG
    AATTACAAGT GTAATGTGTG TTCTCGAACA TTCTTCTCTG AAAATGGGCT CCGAGAACAC
    ATGCAGACAC ACTTGGGACC AGTGAAACAT TACATGTGCC CAATCTGTGG CGAGCGGTTT
    CCATCTCTTC TGACTCTTAC TGAACATAAA GTCACACATA GTAAGAGCCT GGACACCGGA
    AACTGCAGGA TTTGCAAGAT GCCTCTCCAG AGTGAGGAGG AATTTTTAGA GCATTGCCAG
    ATGCACCCTG ACTTGAGGAA CTCTTTGACA GGGTTCCGCT GTGTGGTGTG CATGCAGACT
    GTGACCTCCA CGTTGGAACT GAAAATCCAC GGCACATTCC ACATGCAGAA AACTGGAAAT
    GGTTCTGCCG TGCAGTCCAC GGGGCGCGCA CAGCATCTCC AGAAACTGTA CAAGTGTGCT
    TCTTGCCTGA AGGAATTTCG TTCAAAACAG GATTTAGTGA AACTTGACAT CAATGGTCTG
    CCATATGGTC TGTGTGCTAG CTGTGTTAAT CTCAGTAAAA GTGGTAGTCC AAGTGTCAAC
    ATCCCATCAA GCAGTAACAG ACAAGGCATG GGCCAAAATG AGAACTTGAG TTCCATTGAG
    AACAAAAGCA AGGCAGGGGG ACTGAAGACT CGATGTTCTA GTTGCAATGT TAAATTTGAA
    TCAGAAAGTG AACTCCAAAA CCATATTCAG TCAATCCACA GAGAGCTTGT TCCAGACAGC
    AACAGTACAC AGCTGAAAAC ACCACAAGTA TCTCCAATGC CCAGAATCAG CCCCTCCCAA
    ACTGAAGAGA AGAAGACCTA CCAGTGCATC AAATGTCAGA TGGTTTTCTA CAATGAATGG
    GATATCCAAG TTCATGTTGC AAACCACATG ATTGATGAAG GACTGAACCA TGAATGCAAG
    CTCTGCAATC AGACATTTGA CTCTCCTGCC AAACTTCAGT GCCACCTGAT AGAGCACAGC
    TTTGAAGGCA TGGGGGGCAC CTTCAAATGT CCAGTGTGCT TTACAGTGTT TGTCCAAGCA
    AACAAGTTGC AGCAGCACAT TTTCTCAGCC CATGGACAAG AGGACAAGAT CTATGACTGC
    ACACAGTGTC CACAGAAGTT CTTCTTCCAA ACAGAGCTGC AGAATCATAC GATGACCCAG
    CATAGCAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa146359
    Clone ID Related Accession (Same CDS sequence) XM_025147905.1
    Accession Version XM_025147905.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3990bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product zinc finger protein 521 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAAGCCTT GGGGTTGGCG GAGGAGGAAA AGAGTAGAAG AAAATGAAAC AGAGGACCTG 
    CAGGCAGGTG TGGGACACAC AGCCGCGCAG ACTGACCCTA ACTGTAAACT AGAAGACAAG
    ACAGAAGATG GAGAAGTGTT AGACTGTAAG AAAAGGCCGG ATGAAGGGGA GGAGTTGGAA
    GAAGAAGCCG TGCACAGCTG TGACAGCTGC CTCCAGGTGT TTGAGTCGCT GAGTGATATC
    ACAGAACACA AGATTAATCA ATGTCAACTG ACAGATGGAG TGGATGTTGA GGATGACCCC
    ACTTGCTCAT GGCCAGCATC ATCGCCTTCC AGCAAGGACC AGACTTCCCC AAGCCATGGA
    GAAGGTTGTG ATTTTGGTGA GGAGGAAGGA GGCCCAGGGT TGCCCTATCA GTGCCAGTTT
    TGTGACAAAT CGTTCAGCCG CCTCAGCTAC TTAAAGCACC ACGAGCAAAG TCACAGTGAC
    AAACTCCCTT TCAAATGCAC TTATTGCAGT CGTCTCTTCA AACACAAACG AAGCAGGGAC
    CGCCACATAA AGCTTCATAC GGGAGACAAG AAGTATCACT GCAGTGAGTG TGATGCAGCA
    TTCTCAAGGA GCGATCATCT TAAAATTCAC TTAAAGACTC ATACGTCCAA CAAGCCATAT
    AAATGTGCCA TTTGTAGACG TGGATTCCTG TCATCCAGTT CGCTGCACGG CCACATGCAG
    GTTCACGAGA GGAACAAGGA TGGCTCTCAG TCTGCTTCCC GGATGGAGGA CTGGAAAATG
    AAGGACACTC AGAAGTGCAG TCAGTGTGAA GAAGGCTTTG ATTTTCCTGA AGATCTTCAG
    AAGCACATTG CGGAATGTCA CCCTGAGTGT TCTCCTAATG AAGACCGATC TGCTCTCCAG
    TGTGTTTACT GTCACGAACT CTTTGTAGAG GAAACGTCTC TGGTGAATCA CATGGAGCAG
    GCTCATAATG GTGAGAAGAA GAATTCATGC AGCATTTGCT CTGAGAACTT TCACACTGTG
    GAAGAGCTGT ACAGCCACAT GGACAGTCAC CAGCAGCCAG AATCATGCAA CCACAGCAAC
    AGCCCATCAT TGGTAACTGT TGGCTACACC TCAGTCTCAA GTACCACTCC AGATTCCAAT
    CTCTCTGTGG ATAGTTCAAC AATGGTGGAA ACGGCTCCCC CTATACCAAA AGGCCGTGGA
    AGAAAGCGGG CAGCTCAGCA AGTGCCAGAT ATCACTGGTC CTTCAAGTAA ACAGGCAAAA
    GTTACCTATA GCTGCATTTA TTGCAACAAA CAGTTATTTT CCAGCCTTGC AGTTTTGCAG
    ATACACCTGA AAACTATGCA TTTAGATAAA CCTGAACAAG CCCATATCTG TCAGTATTGT
    TTGGAGGTAT TGCCATCTCT CTACAATCTG AACGAACATC TTAAGCAAGT CCACGAAGCT
    CCGGATCCAG GGCTGATTGT TTCCACTATG CCTGCCATGG TGTACCAGTG CAACTTCTGC
    TCTGAAGTGT TCAATGACCT CAACACGCTT CAAGAACATA TCCGATGTTC TCATGGATTT
    GCCAACCCTG CTGCTAAGGA CAGTAATGCG TTCTTTTGCC CCCATTGCTA CATGGGGTTT
    CTTACAGATT CTTCTCTGGA AGAGCATATT AGACAAGTCC ATTGTGATCT TAGCAGTTCC
    CGTTTTGGTT CCCCTGTGCT CGGAACCCCA AAAGATCCAG TAGTGGAAGT GTATTCTTGT
    TCTTACTGTA CAAATTCTCC AATATTTAAT AGCGTTCTTA AACTGAACAA GCATATCAAG
    GAGAACCATA AGAACATTCC TTTGGCACTG AATTACATCC ACAATGGAAA AAAATCCAGA
    GCCATGAGTC CCTTATCTCC TGTAACGATT GAGCAGACCT CTTTAAAAAT GATGCAGGCG
    GTTGGAGGTG CTCCTCCTCG TCCTGCAGGT GAATATATTT GTAATCAATG TGGTGCTAAG
    TATACTTCTT TGGACGGTTT TCAGACTCAT TTAAAAACAC ATCTTGATAC TGTCCTGCCA
    AAACTGACCT GCCCTCAGTG CAACAAGGAG TTTCCAAATC AGGAGTCTCT GCTGAAGCAT
    GTTACCATTC ATTTTATGAT CACTTCAACC TACTACATCT GTGAAAGCTG TGACAAGCAG
    TTTACTTCTG TGGATGACTT GCAGAAGCAC CTACTAGACA TGCATACATT TGTATTCTTC
    CGCTGCACCT TGTGCCAAGA GGTTTTTGAC TCCAAAGTCT CCATCCAACT ACACTTGGCT
    GTGAAGCACA GCAATGAAAA GAAGGTATAT AGGTGTACGT CTTGCAACTG GGATTTCCGC
    AATGAGACTG ACCTACAGCT TCACGTTAAA CACAACCACT TGGAGAACCA AGGCAAAGTG
    CACAAGTGCA TCTTCTGTGG TGAGTCCTTT GGTACAGAGG TGGAGCTGCA GTGCCATATT
    ACTACGCACA GCAAGAAGTA CAACTGTAAG TTCTGCAGCA AAGCTTTCCA TGCCATCATC
    CTGCTGGAAA AGCATTTAAG GGAAAAACAT TGTGTTTTTG AAACAAAAAC TCCAAATTGT
    GGAACGAATG GGGCATCTGA ACAGGTTCAG AAGGAAGAAG TGGAGCTCCA GACCTTGTTG
    ACAAATAGCC AGGAGTCCCA TAACAGCCAT GATGGCAGCG AAGAGGATGT GGACACATCT
    GAGCCTATGT ATGGCTGTGA CATTTGTGGA GCGGCTTACA CCATGGAGAC TCTCCTGCAA
    AACCACCAGC TTCGAGACCA CAACATTCGA CCTGGAGAAA GTGCCATAGT GAAGAAAAAG
    GCTGAACTCA TTAAAGGGAA TTACAAGTGT AATGTGTGTT CTCGAACATT CTTCTCTGAA
    AATGGGCTCC GAGAACACAT GCAGACACAC TTGGGACCAG TGAAACATTA CATGTGCCCA
    ATCTGTGGCG AGCGGTTTCC ATCTCTTCTG ACTCTTACTG AACATAAAGT CACACATAGT
    AAGAGCCTGG ACACCGGAAA CTGCAGGATT TGCAAGATGC CTCTCCAGAG TGAGGAGGAA
    TTTTTAGAGC ATTGCCAGAT GCACCCTGAC TTGAGGAACT CTTTGACAGG GTTCCGCTGT
    GTGGTGTGCA TGCAGACTGT GACCTCCACG TTGGAACTGA AAATCCACGG CACATTCCAC
    ATGCAGAAAA CTGGAAATGG TTCTGCCGTG CAGTCCACGG GGCGCGCACA GCATCTCCAG
    AAACTGTACA AGTGTGCTTC TTGCCTGAAG GAATTTCGTT CAAAACAGGA TTTAGTGAAA
    CTTGACATCA ATGGTCTGCC ATATGGTCTG TGTGCTAGCT GTGTTAATCT CAGTAAAAGT
    GGTAGTCCAA GTGTCAACAT CCCATCAAGC AGTAACAGAC AAGGCATGGG CCAAAATGAG
    AACTTGAGTT CCATTGAGAA CAAAAGCAAG GCAGGGGGAC TGAAGACTCG ATGTTCTAGT
    TGCAATGTTA AATTTGAATC AGAAAGTGAA CTCCAAAACC ATATTCAGTC AATCCACAGA
    GAGCTTGTTC CAGACAGCAA CAGTACACAG CTGAAAACAC CACAAGTATC TCCAATGCCC
    AGAATCAGCC CCTCCCAAAC TGAAGAGAAG AAGACCTACC AGTGCATCAA ATGTCAGATG
    GTTTTCTACA ATGAATGGGA TATCCAAGTT CATGTTGCAA ACCACATGAT TGATGAAGGA
    CTGAACCATG AATGCAAGCT CTGCAATCAG ACATTTGACT CTCCTGCCAA ACTTCAGTGC
    CACCTGATAG AGCACAGCTT TGAAGGCATG GGGGGCACCT TCAAATGTCC AGTGTGCTTT
    ACAGTGTTTG TCCAAGCAAA CAAGTTGCAG CAGCACATTT TCTCAGCCCA TGGACAAGAG
    GACAAGATCT ATGACTGCAC ACAGTGTCCA CAGAAGTTCT TCTTCCAAAC AGAGCTGCAG
    AATCATACGA TGACCCAGCA TAGCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025003673.1
    CDS1022..5011
    Translation

    Target ORF information:

    RefSeq Version XM_025147905.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025147905.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAAGCCTT GGGGTTGGCG GAGGAGGAAA AGAGTAGAAG AAAATGAAAC AGAGGACCTG 
    CAGGCAGGTG TGGGACACAC AGCCGCGCAG ACTGACCCTA ACTGTAAACT AGAAGACAAG
    ACAGAAGATG GAGAAGTGTT AGACTGTAAG AAAAGGCCGG ATGAAGGGGA GGAGTTGGAA
    GAAGAAGCCG TGCACAGCTG TGACAGCTGC CTCCAGGTGT TTGAGTCGCT GAGTGATATC
    ACAGAACACA AGATTAATCA ATGTCAACTG ACAGATGGAG TGGATGTTGA GGATGACCCC
    ACTTGCTCAT GGCCAGCATC ATCGCCTTCC AGCAAGGACC AGACTTCCCC AAGCCATGGA
    GAAGGTTGTG ATTTTGGTGA GGAGGAAGGA GGCCCAGGGT TGCCCTATCA GTGCCAGTTT
    TGTGACAAAT CGTTCAGCCG CCTCAGCTAC TTAAAGCACC ACGAGCAAAG TCACAGTGAC
    AAACTCCCTT TCAAATGCAC TTATTGCAGT CGTCTCTTCA AACACAAACG AAGCAGGGAC
    CGCCACATAA AGCTTCATAC GGGAGACAAG AAGTATCACT GCAGTGAGTG TGATGCAGCA
    TTCTCAAGGA GCGATCATCT TAAAATTCAC TTAAAGACTC ATACGTCCAA CAAGCCATAT
    AAATGTGCCA TTTGTAGACG TGGATTCCTG TCATCCAGTT CGCTGCACGG CCACATGCAG
    GTTCACGAGA GGAACAAGGA TGGCTCTCAG TCTGCTTCCC GGATGGAGGA CTGGAAAATG
    AAGGACACTC AGAAGTGCAG TCAGTGTGAA GAAGGCTTTG ATTTTCCTGA AGATCTTCAG
    AAGCACATTG CGGAATGTCA CCCTGAGTGT TCTCCTAATG AAGACCGATC TGCTCTCCAG
    TGTGTTTACT GTCACGAACT CTTTGTAGAG GAAACGTCTC TGGTGAATCA CATGGAGCAG
    GCTCATAATG GTGAGAAGAA GAATTCATGC AGCATTTGCT CTGAGAACTT TCACACTGTG
    GAAGAGCTGT ACAGCCACAT GGACAGTCAC CAGCAGCCAG AATCATGCAA CCACAGCAAC
    AGCCCATCAT TGGTAACTGT TGGCTACACC TCAGTCTCAA GTACCACTCC AGATTCCAAT
    CTCTCTGTGG ATAGTTCAAC AATGGTGGAA ACGGCTCCCC CTATACCAAA AGGCCGTGGA
    AGAAAGCGGG CAGCTCAGCA AGTGCCAGAT ATCACTGGTC CTTCAAGTAA ACAGGCAAAA
    GTTACCTATA GCTGCATTTA TTGCAACAAA CAGTTATTTT CCAGCCTTGC AGTTTTGCAG
    ATACACCTGA AAACTATGCA TTTAGATAAA CCTGAACAAG CCCATATCTG TCAGTATTGT
    TTGGAGGTAT TGCCATCTCT CTACAATCTG AACGAACATC TTAAGCAAGT CCACGAAGCT
    CCGGATCCAG GGCTGATTGT TTCCACTATG CCTGCCATGG TGTACCAGTG CAACTTCTGC
    TCTGAAGTGT TCAATGACCT CAACACGCTT CAAGAACATA TCCGATGTTC TCATGGATTT
    GCCAACCCTG CTGCTAAGGA CAGTAATGCG TTCTTTTGCC CCCATTGCTA CATGGGGTTT
    CTTACAGATT CTTCTCTGGA AGAGCATATT AGACAAGTCC ATTGTGATCT TAGCAGTTCC
    CGTTTTGGTT CCCCTGTGCT CGGAACCCCA AAAGATCCAG TAGTGGAAGT GTATTCTTGT
    TCTTACTGTA CAAATTCTCC AATATTTAAT AGCGTTCTTA AACTGAACAA GCATATCAAG
    GAGAACCATA AGAACATTCC TTTGGCACTG AATTACATCC ACAATGGAAA AAAATCCAGA
    GCCATGAGTC CCTTATCTCC TGTAACGATT GAGCAGACCT CTTTAAAAAT GATGCAGGCG
    GTTGGAGGTG CTCCTCCTCG TCCTGCAGGT GAATATATTT GTAATCAATG TGGTGCTAAG
    TATACTTCTT TGGACGGTTT TCAGACTCAT TTAAAAACAC ATCTTGATAC TGTCCTGCCA
    AAACTGACCT GCCCTCAGTG CAACAAGGAG TTTCCAAATC AGGAGTCTCT GCTGAAGCAT
    GTTACCATTC ATTTTATGAT CACTTCAACC TACTACATCT GTGAAAGCTG TGACAAGCAG
    TTTACTTCTG TGGATGACTT GCAGAAGCAC CTACTAGACA TGCATACATT TGTATTCTTC
    CGCTGCACCT TGTGCCAAGA GGTTTTTGAC TCCAAAGTCT CCATCCAACT ACACTTGGCT
    GTGAAGCACA GCAATGAAAA GAAGGTATAT AGGTGTACGT CTTGCAACTG GGATTTCCGC
    AATGAGACTG ACCTACAGCT TCACGTTAAA CACAACCACT TGGAGAACCA AGGCAAAGTG
    CACAAGTGCA TCTTCTGTGG TGAGTCCTTT GGTACAGAGG TGGAGCTGCA GTGCCATATT
    ACTACGCACA GCAAGAAGTA CAACTGTAAG TTCTGCAGCA AAGCTTTCCA TGCCATCATC
    CTGCTGGAAA AGCATTTAAG GGAAAAACAT TGTGTTTTTG AAACAAAAAC TCCAAATTGT
    GGAACGAATG GGGCATCTGA ACAGGTTCAG AAGGAAGAAG TGGAGCTCCA GACCTTGTTG
    ACAAATAGCC AGGAGTCCCA TAACAGCCAT GATGGCAGCG AAGAGGATGT GGACACATCT
    GAGCCTATGT ATGGCTGTGA CATTTGTGGA GCGGCTTACA CCATGGAGAC TCTCCTGCAA
    AACCACCAGC TTCGAGACCA CAACATTCGA CCTGGAGAAA GTGCCATAGT GAAGAAAAAG
    GCTGAACTCA TTAAAGGGAA TTACAAGTGT AATGTGTGTT CTCGAACATT CTTCTCTGAA
    AATGGGCTCC GAGAACACAT GCAGACACAC TTGGGACCAG TGAAACATTA CATGTGCCCA
    ATCTGTGGCG AGCGGTTTCC ATCTCTTCTG ACTCTTACTG AACATAAAGT CACACATAGT
    AAGAGCCTGG ACACCGGAAA CTGCAGGATT TGCAAGATGC CTCTCCAGAG TGAGGAGGAA
    TTTTTAGAGC ATTGCCAGAT GCACCCTGAC TTGAGGAACT CTTTGACAGG GTTCCGCTGT
    GTGGTGTGCA TGCAGACTGT GACCTCCACG TTGGAACTGA AAATCCACGG CACATTCCAC
    ATGCAGAAAA CTGGAAATGG TTCTGCCGTG CAGTCCACGG GGCGCGCACA GCATCTCCAG
    AAACTGTACA AGTGTGCTTC TTGCCTGAAG GAATTTCGTT CAAAACAGGA TTTAGTGAAA
    CTTGACATCA ATGGTCTGCC ATATGGTCTG TGTGCTAGCT GTGTTAATCT CAGTAAAAGT
    GGTAGTCCAA GTGTCAACAT CCCATCAAGC AGTAACAGAC AAGGCATGGG CCAAAATGAG
    AACTTGAGTT CCATTGAGAA CAAAAGCAAG GCAGGGGGAC TGAAGACTCG ATGTTCTAGT
    TGCAATGTTA AATTTGAATC AGAAAGTGAA CTCCAAAACC ATATTCAGTC AATCCACAGA
    GAGCTTGTTC CAGACAGCAA CAGTACACAG CTGAAAACAC CACAAGTATC TCCAATGCCC
    AGAATCAGCC CCTCCCAAAC TGAAGAGAAG AAGACCTACC AGTGCATCAA ATGTCAGATG
    GTTTTCTACA ATGAATGGGA TATCCAAGTT CATGTTGCAA ACCACATGAT TGATGAAGGA
    CTGAACCATG AATGCAAGCT CTGCAATCAG ACATTTGACT CTCCTGCCAA ACTTCAGTGC
    CACCTGATAG AGCACAGCTT TGAAGGCATG GGGGGCACCT TCAAATGTCC AGTGTGCTTT
    ACAGTGTTTG TCCAAGCAAA CAAGTTGCAG CAGCACATTT TCTCAGCCCA TGGACAAGAG
    GACAAGATCT ATGACTGCAC ACAGTGTCCA CAGAAGTTCT TCTTCCAAAC AGAGCTGCAG
    AATCATACGA TGACCCAGCA TAGCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32510
    Clone ID Related Accession (Same CDS sequence) XM_004939804.2 , XM_004939804.3
    Accession Version XM_004939804.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product zinc finger protein 521 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004939804.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCTTTGG GGGCCTTGCT TCCATTTTCC ATTATTATGT GGACTACCGG AGCGACAGCG 
    CAGTCCAAGA CCTTGCAGCT GTTTGCTTTT AGGATGTCTC GCCGCAAGCA AGCTAAACCG
    AGATCACTCA AAGAAACCCA GATTCTAGAA ACAACCAATT GTATGAAGCC TTGGGGTTGG
    CGGAGGAGGA AAAGAGTAGA AGAAAATGAA ACAGAGGACC TGCAGGCAGG TGTGGGACAC
    ACAGCCGCGC AGACTGACCC TAACTGTAAA CTAGAAGACA AGACAGAAGA TGGAGAAGTG
    TTAGACTGTA AGAAAAGGCC GGATGAAGGG GAGGAGTTGG AAGAAGAAGC CGTGCACAGC
    TGTGACAGCT GCCTCCAGGT GTTTGAGTCG CTGAGTGATA TCACAGAACA CAAGATTAAT
    CAATGTCAAC TGACAGATGG AGTGGATGTT GAGGATGACC CCACTTGCTC ATGGCCAGCA
    TCATCGCCTT CCAGCAAGGA CCAGACTTCC CCAAGCCATG GAGAAGGTTG TGATTTTGGT
    GAGGAGGAAG GAGGCCCAGG GTTGCCCTAT CAGTGCCAGT TTTGTGACAA ATCGTTCAGC
    CGCCTCAGCT ACTTAAAGCA CCACGAGCAA AGTCACAGTG ACAAACTCCC TTTCAAATGC
    ACTTATTGCA GTCGTCTCTT CAAACACAAA CGAAGCAGGG ACCGCCACAT AAAGCTTCAT
    ACGGGAGACA AGAAGTATCA CTGCAGTGAG TGTGATGCAG CATTCTCAAG GAGCGATCAT
    CTTAAAATTC ACTTAAAGAC TCATACGTCC AACAAGCCAT ATAAATGTGC CATTTGTAGA
    CGTGGATTCC TGTCATCCAG TTCGCTGCAC GGCCACATGC AGGTTCACGA GAGGAACAAG
    GATGGCTCTC AGTCTGCTTC CCGGATGGAG GACTGGAAAA TGAAGGACAC TCAGAAGTGC
    AGTCAGTGTG AAGAAGGCTT TGATTTTCCT GAAGATCTTC AGAAGCACAT TGCGGAATGT
    CACCCTGAGT GTTCTCCTAA TGAAGACCGA TCTGCTCTCC AGTGTGTTTA CTGTCACGAA
    CTCTTTGTAG AGGAAACGTC TCTGGTGAAT CACATGGAGC AGGCTCATAA TGGTGAGAAG
    AAGAATTCAT GCAGCATTTG CTCTGAGAAC TTTCACACTG TGGAAGAGCT GTACAGCCAC
    ATGGACAGTC ACCAGCAGCC AGAATCATGC AACCACAGCA ACAGCCCATC ATTGGTAACT
    GTTGGCTACA CCTCAGTCTC AAGTACCACT CCAGATTCCA ATCTCTCTGT GGATAGTTCA
    ACAATGGTGG AAACGGCTCC CCCTATACCA AAAGGCCGTG GAAGAAAGCG GGCAGCTCAG
    CAAGTGCCAG ATATCACTGG TCCTTCAAGT AAACAGGCAA AAGTTACCTA TAGCTGCATT
    TATTGCAACA AACAGTTATT TTCCAGCCTT GCAGTTTTGC AGATACACCT GAAAACTATG
    CATTTAGATA AACCTGAACA AGCCCATATC TGTCAGTATT GTTTGGAGGT ATTGCCATCT
    CTCTACAATC TGAACGAACA TCTTAAGCAA GTCCACGAAG CTCCGGATCC AGGGCTGATT
    GTTTCCACTA TGCCTGCCAT GGTGTACCAG TGCAACTTCT GCTCTGAAGT GTTCAATGAC
    CTCAACACGC TTCAAGAACA TATCCGATGT TCTCATGGAT TTGCCAACCC TGCTGCTAAG
    GACAGTAATG CGTTCTTTTG CCCCCATTGC TACATGGGGT TTCTTACAGA TTCTTCTCTG
    GAAGAGCATA TTAGACAAGT CCATTGTGAT CTTAGCAGTT CCCGTTTTGG TTCCCCTGTG
    CTCGGAACCC CAAAAGATCC AGTAGTGGAA GTGTATTCTT GTTCTTACTG TACAAATTCT
    CCAATATTTA ATAGCGTTCT TAAACTGAAC AAGCATATCA AGGAGAACCA TAAGAACATT
    CCTTTGGCAC TGAATTACAT CCACAATGGA AAAAAATCCA GAGCCATGAG TCCCTTATCT
    CCTGTAACGA TTGAGCAGAC CTCTTTAAAA ATGATGCAGG CGGTTGGAGG TGCTCCTCCT
    CGTCCTGCAG GTGAATATAT TTGTAATCAA TGTGGTGCTA AGTATACTTC TTTGGACGGT
    TTTCAGACTC ATTTAAAAAC ACATCTTGAT ACTGTCCTGC CAAAACTGAC CTGCCCTCAG
    TGCAACAAGG AGTTTCCAAA TCAGGAGTCT CTGCTGAAGC ATGTTACCAT TCATTTTATG
    ATCACTTCAA CCTACTACAT CTGTGAAAGC TGTGACAAGC AGTTTACTTC TGTGGATGAC
    TTGCAGAAGC ACCTACTAGA CATGCATACA TTTGTATTCT TCCGCTGCAC CTTGTGCCAA
    GAGGTTTTTG ACTCCAAAGT CTCCATCCAA CTACACTTGG CTGTGAAGCA CAGCAATGAA
    AAGAAGGTAT ATAGGTGTAC GTCTTGCAAC TGGGATTTCC GCAATGAGAC TGACCTACAG
    CTTCACGTTA AACACAACCA CTTGGAGAAC CAAGGCAAAG TGCACAAGTG CATCTTCTGT
    GGTGAGTCCT TTGGTACAGA GGTGGAGCTG CAGTGCCATA TTACTACGCA CAGCAAGAAG
    TACAACTGTA AGTTCTGCAG CAAAGCTTTC CATGCCATCA TCCTGCTGGA AAAGCATTTA
    AGGGAAAAAC ATTGTGTTTT TGAAACAAAA ACTCCAAATT GTGGAACGAA TGGGGCATCT
    GAACAGGTTC AGAAGGAAGA AGTGGAGCTC CAGACCTTGT TGACAAATAG CCAGGAGTCC
    CATAACAGCC ATGATGGCAG CGAAGAGGAT GTGGACACAT CTGAGCCTAT GTATGGCTGT
    GACATTTGTG GAGCGGCTTA CACCATGGAG ACTCTCCTGC AAAACCACCA GCTTCGAGAC
    CACAACATTC GACCTGGAGA AAGTGCCATA GTGAAGAAAA AGGCTGAACT CATTAAAGGG
    AATTACAAGT GTAATGTGTG TTCTCGAACA TTCTTCTCTG AAAATGGGCT CCGAGAACAC
    ATGCAGACAC ACTTGGGACC AGTGAAACAT TACATGTGCC CAATCTGTGG CGAGCGGTTT
    CCATCTCTTC TGACTCTTAC TGAACATAAA GTCACACATA GTAAGAGCCT GGACACCGGA
    AACTGCAGGA TTTGCAAGAT GCCTCTCCAG AGTGAGGAGG AATTTTTAGA GCATTGCCAG
    ATGCACCCTG ACTTGAGGAA CTCTTTGACA GGGTTCCGCT GTGTGGTGTG CATGCAGACT
    GTGACCTCCA CGTTGGAACT GAAAATCCAC GGCACATTCC ACATGCAGAA AACTGGAAAT
    GGTTCTGCCG TGCAGTCCAC GGGGCGCGCA CAGCATCTCC AGAAACTGTA CAAGTGTGCT
    TCTTGCCTGA AGGAATTTCG TTCAAAACAG GATTTAGTGA AACTTGACAT CAATGGTCTG
    CCATATGGTC TGTGTGCTAG CTGTGTTAAT CTCAGTAAAA GTGGTAGTCC AAGTGTCAAC
    ATCCCATCAA GCAGTAACAG ACAAGGCATG GGCCAAAATG AGAACTTGAG TTCCATTGAG
    AACAAAAGCA AGGCAGGGGG ACTGAAGACT CGATGTTCTA GTTGCAATGT TAAATTTGAA
    TCAGAAAGTG AACTCCAAAA CCATATTCAG TCAATCCACA GAGAGCTTGT TCCAGACAGC
    AACAGTACAC AGCTGAAAAC ACCACAAGTA TCTCCAATGC CCAGAATCAG CCCCTCCCAA
    ACTGAAGAGA AGACCTACCA GTGCATCAAA TGTCAGATGG TTTTCTACAA TGAATGGGAT
    ATCCAAGTTC ATGTTGCAAA CCACATGATT GATGAAGGAC TGAACCATGA ATGCAAGCTC
    TGCAATCAGA CATTTGACTC TCCTGCCAAA CTTCAGTGCC ACCTGATAGA GCACAGCTTT
    GAAGGCATGG GGGGCACCTT CAAATGTCCA GTGTGCTTTA CAGTGTTTGT CCAAGCAAAC
    AAGTTGCAGC AGCACATTTT CTCAGCCCAT GGACAAGAGG ACAAGATCTA TGACTGCACA
    CAGTGTCCAC AGAAGTTCTT CTTCCAAACA GAGCTGCAGA ATCATACGAT GACCCAGCAT
    AGCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004939861.2
    CDS5..4153
    Translation

    Target ORF information:

    RefSeq Version XM_004939804.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004939804.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCTTTGG GGGCCTTGCT TCCATTTTCC ATTATTATGT GGACTACCGG AGCGACAGCG 
    CAGTCCAAGA CCTTGCAGCT GTTTGCTTTT AGGATGTCTC GCCGCAAGCA AGCTAAACCG
    AGATCACTCA AAGAAACCCA GATTCTAGAA ACAACCAATT GTATGAAGCC TTGGGGTTGG
    CGGAGGAGGA AAAGAGTAGA AGAAAATGAA ACAGAGGACC TGCAGGCAGG TGTGGGACAC
    ACAGCCGCGC AGACTGACCC TAACTGTAAA CTAGAAGACA AGACAGAAGA TGGAGAAGTG
    TTAGACTGTA AGAAAAGGCC GGATGAAGGG GAGGAGTTGG AAGAAGAAGC CGTGCACAGC
    TGTGACAGCT GCCTCCAGGT GTTTGAGTCG CTGAGTGATA TCACAGAACA CAAGATTAAT
    CAATGTCAAC TGACAGATGG AGTGGATGTT GAGGATGACC CCACTTGCTC ATGGCCAGCA
    TCATCGCCTT CCAGCAAGGA CCAGACTTCC CCAAGCCATG GAGAAGGTTG TGATTTTGGT
    GAGGAGGAAG GAGGCCCAGG GTTGCCCTAT CAGTGCCAGT TTTGTGACAA ATCGTTCAGC
    CGCCTCAGCT ACTTAAAGCA CCACGAGCAA AGTCACAGTG ACAAACTCCC TTTCAAATGC
    ACTTATTGCA GTCGTCTCTT CAAACACAAA CGAAGCAGGG ACCGCCACAT AAAGCTTCAT
    ACGGGAGACA AGAAGTATCA CTGCAGTGAG TGTGATGCAG CATTCTCAAG GAGCGATCAT
    CTTAAAATTC ACTTAAAGAC TCATACGTCC AACAAGCCAT ATAAATGTGC CATTTGTAGA
    CGTGGATTCC TGTCATCCAG TTCGCTGCAC GGCCACATGC AGGTTCACGA GAGGAACAAG
    GATGGCTCTC AGTCTGCTTC CCGGATGGAG GACTGGAAAA TGAAGGACAC TCAGAAGTGC
    AGTCAGTGTG AAGAAGGCTT TGATTTTCCT GAAGATCTTC AGAAGCACAT TGCGGAATGT
    CACCCTGAGT GTTCTCCTAA TGAAGACCGA TCTGCTCTCC AGTGTGTTTA CTGTCACGAA
    CTCTTTGTAG AGGAAACGTC TCTGGTGAAT CACATGGAGC AGGCTCATAA TGGTGAGAAG
    AAGAATTCAT GCAGCATTTG CTCTGAGAAC TTTCACACTG TGGAAGAGCT GTACAGCCAC
    ATGGACAGTC ACCAGCAGCC AGAATCATGC AACCACAGCA ACAGCCCATC ATTGGTAACT
    GTTGGCTACA CCTCAGTCTC AAGTACCACT CCAGATTCCA ATCTCTCTGT GGATAGTTCA
    ACAATGGTGG AAACGGCTCC CCCTATACCA AAAGGCCGTG GAAGAAAGCG GGCAGCTCAG
    CAAGTGCCAG ATATCACTGG TCCTTCAAGT AAACAGGCAA AAGTTACCTA TAGCTGCATT
    TATTGCAACA AACAGTTATT TTCCAGCCTT GCAGTTTTGC AGATACACCT GAAAACTATG
    CATTTAGATA AACCTGAACA AGCCCATATC TGTCAGTATT GTTTGGAGGT ATTGCCATCT
    CTCTACAATC TGAACGAACA TCTTAAGCAA GTCCACGAAG CTCCGGATCC AGGGCTGATT
    GTTTCCACTA TGCCTGCCAT GGTGTACCAG TGCAACTTCT GCTCTGAAGT GTTCAATGAC
    CTCAACACGC TTCAAGAACA TATCCGATGT TCTCATGGAT TTGCCAACCC TGCTGCTAAG
    GACAGTAATG CGTTCTTTTG CCCCCATTGC TACATGGGGT TTCTTACAGA TTCTTCTCTG
    GAAGAGCATA TTAGACAAGT CCATTGTGAT CTTAGCAGTT CCCGTTTTGG TTCCCCTGTG
    CTCGGAACCC CAAAAGATCC AGTAGTGGAA GTGTATTCTT GTTCTTACTG TACAAATTCT
    CCAATATTTA ATAGCGTTCT TAAACTGAAC AAGCATATCA AGGAGAACCA TAAGAACATT
    CCTTTGGCAC TGAATTACAT CCACAATGGA AAAAAATCCA GAGCCATGAG TCCCTTATCT
    CCTGTAACGA TTGAGCAGAC CTCTTTAAAA ATGATGCAGG CGGTTGGAGG TGCTCCTCCT
    CGTCCTGCAG GTGAATATAT TTGTAATCAA TGTGGTGCTA AGTATACTTC TTTGGACGGT
    TTTCAGACTC ATTTAAAAAC ACATCTTGAT ACTGTCCTGC CAAAACTGAC CTGCCCTCAG
    TGCAACAAGG AGTTTCCAAA TCAGGAGTCT CTGCTGAAGC ATGTTACCAT TCATTTTATG
    ATCACTTCAA CCTACTACAT CTGTGAAAGC TGTGACAAGC AGTTTACTTC TGTGGATGAC
    TTGCAGAAGC ACCTACTAGA CATGCATACA TTTGTATTCT TCCGCTGCAC CTTGTGCCAA
    GAGGTTTTTG ACTCCAAAGT CTCCATCCAA CTACACTTGG CTGTGAAGCA CAGCAATGAA
    AAGAAGGTAT ATAGGTGTAC GTCTTGCAAC TGGGATTTCC GCAATGAGAC TGACCTACAG
    CTTCACGTTA AACACAACCA CTTGGAGAAC CAAGGCAAAG TGCACAAGTG CATCTTCTGT
    GGTGAGTCCT TTGGTACAGA GGTGGAGCTG CAGTGCCATA TTACTACGCA CAGCAAGAAG
    TACAACTGTA AGTTCTGCAG CAAAGCTTTC CATGCCATCA TCCTGCTGGA AAAGCATTTA
    AGGGAAAAAC ATTGTGTTTT TGAAACAAAA ACTCCAAATT GTGGAACGAA TGGGGCATCT
    GAACAGGTTC AGAAGGAAGA AGTGGAGCTC CAGACCTTGT TGACAAATAG CCAGGAGTCC
    CATAACAGCC ATGATGGCAG CGAAGAGGAT GTGGACACAT CTGAGCCTAT GTATGGCTGT
    GACATTTGTG GAGCGGCTTA CACCATGGAG ACTCTCCTGC AAAACCACCA GCTTCGAGAC
    CACAACATTC GACCTGGAGA AAGTGCCATA GTGAAGAAAA AGGCTGAACT CATTAAAGGG
    AATTACAAGT GTAATGTGTG TTCTCGAACA TTCTTCTCTG AAAATGGGCT CCGAGAACAC
    ATGCAGACAC ACTTGGGACC AGTGAAACAT TACATGTGCC CAATCTGTGG CGAGCGGTTT
    CCATCTCTTC TGACTCTTAC TGAACATAAA GTCACACATA GTAAGAGCCT GGACACCGGA
    AACTGCAGGA TTTGCAAGAT GCCTCTCCAG AGTGAGGAGG AATTTTTAGA GCATTGCCAG
    ATGCACCCTG ACTTGAGGAA CTCTTTGACA GGGTTCCGCT GTGTGGTGTG CATGCAGACT
    GTGACCTCCA CGTTGGAACT GAAAATCCAC GGCACATTCC ACATGCAGAA AACTGGAAAT
    GGTTCTGCCG TGCAGTCCAC GGGGCGCGCA CAGCATCTCC AGAAACTGTA CAAGTGTGCT
    TCTTGCCTGA AGGAATTTCG TTCAAAACAG GATTTAGTGA AACTTGACAT CAATGGTCTG
    CCATATGGTC TGTGTGCTAG CTGTGTTAAT CTCAGTAAAA GTGGTAGTCC AAGTGTCAAC
    ATCCCATCAA GCAGTAACAG ACAAGGCATG GGCCAAAATG AGAACTTGAG TTCCATTGAG
    AACAAAAGCA AGGCAGGGGG ACTGAAGACT CGATGTTCTA GTTGCAATGT TAAATTTGAA
    TCAGAAAGTG AACTCCAAAA CCATATTCAG TCAATCCACA GAGAGCTTGT TCCAGACAGC
    AACAGTACAC AGCTGAAAAC ACCACAAGTA TCTCCAATGC CCAGAATCAG CCCCTCCCAA
    ACTGAAGAGA AGACCTACCA GTGCATCAAA TGTCAGATGG TTTTCTACAA TGAATGGGAT
    ATCCAAGTTC ATGTTGCAAA CCACATGATT GATGAAGGAC TGAACCATGA ATGCAAGCTC
    TGCAATCAGA CATTTGACTC TCCTGCCAAA CTTCAGTGCC ACCTGATAGA GCACAGCTTT
    GAAGGCATGG GGGGCACCTT CAAATGTCCA GTGTGCTTTA CAGTGTTTGT CCAAGCAAAC
    AAGTTGCAGC AGCACATTTT CTCAGCCCAT GGACAAGAGG ACAAGATCTA TGACTGCACA
    CAGTGTCCAC AGAAGTTCTT CTTCCAAACA GAGCTGCAGA ATCATACGAT GACCCAGCAT
    AGCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32510
    Clone ID Related Accession (Same CDS sequence) XM_004939804.2 , XM_004939804.3
    Accession Version XM_004939804.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product zinc finger protein 521 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004939804.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCTTTGG GGGCCTTGCT TCCATTTTCC ATTATTATGT GGACTACCGG AGCGACAGCG 
    CAGTCCAAGA CCTTGCAGCT GTTTGCTTTT AGGATGTCTC GCCGCAAGCA AGCTAAACCG
    AGATCACTCA AAGAAACCCA GATTCTAGAA ACAACCAATT GTATGAAGCC TTGGGGTTGG
    CGGAGGAGGA AAAGAGTAGA AGAAAATGAA ACAGAGGACC TGCAGGCAGG TGTGGGACAC
    ACAGCCGCGC AGACTGACCC TAACTGTAAA CTAGAAGACA AGACAGAAGA TGGAGAAGTG
    TTAGACTGTA AGAAAAGGCC GGATGAAGGG GAGGAGTTGG AAGAAGAAGC CGTGCACAGC
    TGTGACAGCT GCCTCCAGGT GTTTGAGTCG CTGAGTGATA TCACAGAACA CAAGATTAAT
    CAATGTCAAC TGACAGATGG AGTGGATGTT GAGGATGACC CCACTTGCTC ATGGCCAGCA
    TCATCGCCTT CCAGCAAGGA CCAGACTTCC CCAAGCCATG GAGAAGGTTG TGATTTTGGT
    GAGGAGGAAG GAGGCCCAGG GTTGCCCTAT CAGTGCCAGT TTTGTGACAA ATCGTTCAGC
    CGCCTCAGCT ACTTAAAGCA CCACGAGCAA AGTCACAGTG ACAAACTCCC TTTCAAATGC
    ACTTATTGCA GTCGTCTCTT CAAACACAAA CGAAGCAGGG ACCGCCACAT AAAGCTTCAT
    ACGGGAGACA AGAAGTATCA CTGCAGTGAG TGTGATGCAG CATTCTCAAG GAGCGATCAT
    CTTAAAATTC ACTTAAAGAC TCATACGTCC AACAAGCCAT ATAAATGTGC CATTTGTAGA
    CGTGGATTCC TGTCATCCAG TTCGCTGCAC GGCCACATGC AGGTTCACGA GAGGAACAAG
    GATGGCTCTC AGTCTGCTTC CCGGATGGAG GACTGGAAAA TGAAGGACAC TCAGAAGTGC
    AGTCAGTGTG AAGAAGGCTT TGATTTTCCT GAAGATCTTC AGAAGCACAT TGCGGAATGT
    CACCCTGAGT GTTCTCCTAA TGAAGACCGA TCTGCTCTCC AGTGTGTTTA CTGTCACGAA
    CTCTTTGTAG AGGAAACGTC TCTGGTGAAT CACATGGAGC AGGCTCATAA TGGTGAGAAG
    AAGAATTCAT GCAGCATTTG CTCTGAGAAC TTTCACACTG TGGAAGAGCT GTACAGCCAC
    ATGGACAGTC ACCAGCAGCC AGAATCATGC AACCACAGCA ACAGCCCATC ATTGGTAACT
    GTTGGCTACA CCTCAGTCTC AAGTACCACT CCAGATTCCA ATCTCTCTGT GGATAGTTCA
    ACAATGGTGG AAACGGCTCC CCCTATACCA AAAGGCCGTG GAAGAAAGCG GGCAGCTCAG
    CAAGTGCCAG ATATCACTGG TCCTTCAAGT AAACAGGCAA AAGTTACCTA TAGCTGCATT
    TATTGCAACA AACAGTTATT TTCCAGCCTT GCAGTTTTGC AGATACACCT GAAAACTATG
    CATTTAGATA AACCTGAACA AGCCCATATC TGTCAGTATT GTTTGGAGGT ATTGCCATCT
    CTCTACAATC TGAACGAACA TCTTAAGCAA GTCCACGAAG CTCCGGATCC AGGGCTGATT
    GTTTCCACTA TGCCTGCCAT GGTGTACCAG TGCAACTTCT GCTCTGAAGT GTTCAATGAC
    CTCAACACGC TTCAAGAACA TATCCGATGT TCTCATGGAT TTGCCAACCC TGCTGCTAAG
    GACAGTAATG CGTTCTTTTG CCCCCATTGC TACATGGGGT TTCTTACAGA TTCTTCTCTG
    GAAGAGCATA TTAGACAAGT CCATTGTGAT CTTAGCAGTT CCCGTTTTGG TTCCCCTGTG
    CTCGGAACCC CAAAAGATCC AGTAGTGGAA GTGTATTCTT GTTCTTACTG TACAAATTCT
    CCAATATTTA ATAGCGTTCT TAAACTGAAC AAGCATATCA AGGAGAACCA TAAGAACATT
    CCTTTGGCAC TGAATTACAT CCACAATGGA AAAAAATCCA GAGCCATGAG TCCCTTATCT
    CCTGTAACGA TTGAGCAGAC CTCTTTAAAA ATGATGCAGG CGGTTGGAGG TGCTCCTCCT
    CGTCCTGCAG GTGAATATAT TTGTAATCAA TGTGGTGCTA AGTATACTTC TTTGGACGGT
    TTTCAGACTC ATTTAAAAAC ACATCTTGAT ACTGTCCTGC CAAAACTGAC CTGCCCTCAG
    TGCAACAAGG AGTTTCCAAA TCAGGAGTCT CTGCTGAAGC ATGTTACCAT TCATTTTATG
    ATCACTTCAA CCTACTACAT CTGTGAAAGC TGTGACAAGC AGTTTACTTC TGTGGATGAC
    TTGCAGAAGC ACCTACTAGA CATGCATACA TTTGTATTCT TCCGCTGCAC CTTGTGCCAA
    GAGGTTTTTG ACTCCAAAGT CTCCATCCAA CTACACTTGG CTGTGAAGCA CAGCAATGAA
    AAGAAGGTAT ATAGGTGTAC GTCTTGCAAC TGGGATTTCC GCAATGAGAC TGACCTACAG
    CTTCACGTTA AACACAACCA CTTGGAGAAC CAAGGCAAAG TGCACAAGTG CATCTTCTGT
    GGTGAGTCCT TTGGTACAGA GGTGGAGCTG CAGTGCCATA TTACTACGCA CAGCAAGAAG
    TACAACTGTA AGTTCTGCAG CAAAGCTTTC CATGCCATCA TCCTGCTGGA AAAGCATTTA
    AGGGAAAAAC ATTGTGTTTT TGAAACAAAA ACTCCAAATT GTGGAACGAA TGGGGCATCT
    GAACAGGTTC AGAAGGAAGA AGTGGAGCTC CAGACCTTGT TGACAAATAG CCAGGAGTCC
    CATAACAGCC ATGATGGCAG CGAAGAGGAT GTGGACACAT CTGAGCCTAT GTATGGCTGT
    GACATTTGTG GAGCGGCTTA CACCATGGAG ACTCTCCTGC AAAACCACCA GCTTCGAGAC
    CACAACATTC GACCTGGAGA AAGTGCCATA GTGAAGAAAA AGGCTGAACT CATTAAAGGG
    AATTACAAGT GTAATGTGTG TTCTCGAACA TTCTTCTCTG AAAATGGGCT CCGAGAACAC
    ATGCAGACAC ACTTGGGACC AGTGAAACAT TACATGTGCC CAATCTGTGG CGAGCGGTTT
    CCATCTCTTC TGACTCTTAC TGAACATAAA GTCACACATA GTAAGAGCCT GGACACCGGA
    AACTGCAGGA TTTGCAAGAT GCCTCTCCAG AGTGAGGAGG AATTTTTAGA GCATTGCCAG
    ATGCACCCTG ACTTGAGGAA CTCTTTGACA GGGTTCCGCT GTGTGGTGTG CATGCAGACT
    GTGACCTCCA CGTTGGAACT GAAAATCCAC GGCACATTCC ACATGCAGAA AACTGGAAAT
    GGTTCTGCCG TGCAGTCCAC GGGGCGCGCA CAGCATCTCC AGAAACTGTA CAAGTGTGCT
    TCTTGCCTGA AGGAATTTCG TTCAAAACAG GATTTAGTGA AACTTGACAT CAATGGTCTG
    CCATATGGTC TGTGTGCTAG CTGTGTTAAT CTCAGTAAAA GTGGTAGTCC AAGTGTCAAC
    ATCCCATCAA GCAGTAACAG ACAAGGCATG GGCCAAAATG AGAACTTGAG TTCCATTGAG
    AACAAAAGCA AGGCAGGGGG ACTGAAGACT CGATGTTCTA GTTGCAATGT TAAATTTGAA
    TCAGAAAGTG AACTCCAAAA CCATATTCAG TCAATCCACA GAGAGCTTGT TCCAGACAGC
    AACAGTACAC AGCTGAAAAC ACCACAAGTA TCTCCAATGC CCAGAATCAG CCCCTCCCAA
    ACTGAAGAGA AGACCTACCA GTGCATCAAA TGTCAGATGG TTTTCTACAA TGAATGGGAT
    ATCCAAGTTC ATGTTGCAAA CCACATGATT GATGAAGGAC TGAACCATGA ATGCAAGCTC
    TGCAATCAGA CATTTGACTC TCCTGCCAAA CTTCAGTGCC ACCTGATAGA GCACAGCTTT
    GAAGGCATGG GGGGCACCTT CAAATGTCCA GTGTGCTTTA CAGTGTTTGT CCAAGCAAAC
    AAGTTGCAGC AGCACATTTT CTCAGCCCAT GGACAAGAGG ACAAGATCTA TGACTGCACA
    CAGTGTCCAC AGAAGTTCTT CTTCCAAACA GAGCTGCAGA ATCATACGAT GACCCAGCAT
    AGCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004939861.2
    CDS47..4195
    Translation

    Target ORF information:

    RefSeq Version XM_004939804.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004939804.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCTTTGG GGGCCTTGCT TCCATTTTCC ATTATTATGT GGACTACCGG AGCGACAGCG 
    CAGTCCAAGA CCTTGCAGCT GTTTGCTTTT AGGATGTCTC GCCGCAAGCA AGCTAAACCG
    AGATCACTCA AAGAAACCCA GATTCTAGAA ACAACCAATT GTATGAAGCC TTGGGGTTGG
    CGGAGGAGGA AAAGAGTAGA AGAAAATGAA ACAGAGGACC TGCAGGCAGG TGTGGGACAC
    ACAGCCGCGC AGACTGACCC TAACTGTAAA CTAGAAGACA AGACAGAAGA TGGAGAAGTG
    TTAGACTGTA AGAAAAGGCC GGATGAAGGG GAGGAGTTGG AAGAAGAAGC CGTGCACAGC
    TGTGACAGCT GCCTCCAGGT GTTTGAGTCG CTGAGTGATA TCACAGAACA CAAGATTAAT
    CAATGTCAAC TGACAGATGG AGTGGATGTT GAGGATGACC CCACTTGCTC ATGGCCAGCA
    TCATCGCCTT CCAGCAAGGA CCAGACTTCC CCAAGCCATG GAGAAGGTTG TGATTTTGGT
    GAGGAGGAAG GAGGCCCAGG GTTGCCCTAT CAGTGCCAGT TTTGTGACAA ATCGTTCAGC
    CGCCTCAGCT ACTTAAAGCA CCACGAGCAA AGTCACAGTG ACAAACTCCC TTTCAAATGC
    ACTTATTGCA GTCGTCTCTT CAAACACAAA CGAAGCAGGG ACCGCCACAT AAAGCTTCAT
    ACGGGAGACA AGAAGTATCA CTGCAGTGAG TGTGATGCAG CATTCTCAAG GAGCGATCAT
    CTTAAAATTC ACTTAAAGAC TCATACGTCC AACAAGCCAT ATAAATGTGC CATTTGTAGA
    CGTGGATTCC TGTCATCCAG TTCGCTGCAC GGCCACATGC AGGTTCACGA GAGGAACAAG
    GATGGCTCTC AGTCTGCTTC CCGGATGGAG GACTGGAAAA TGAAGGACAC TCAGAAGTGC
    AGTCAGTGTG AAGAAGGCTT TGATTTTCCT GAAGATCTTC AGAAGCACAT TGCGGAATGT
    CACCCTGAGT GTTCTCCTAA TGAAGACCGA TCTGCTCTCC AGTGTGTTTA CTGTCACGAA
    CTCTTTGTAG AGGAAACGTC TCTGGTGAAT CACATGGAGC AGGCTCATAA TGGTGAGAAG
    AAGAATTCAT GCAGCATTTG CTCTGAGAAC TTTCACACTG TGGAAGAGCT GTACAGCCAC
    ATGGACAGTC ACCAGCAGCC AGAATCATGC AACCACAGCA ACAGCCCATC ATTGGTAACT
    GTTGGCTACA CCTCAGTCTC AAGTACCACT CCAGATTCCA ATCTCTCTGT GGATAGTTCA
    ACAATGGTGG AAACGGCTCC CCCTATACCA AAAGGCCGTG GAAGAAAGCG GGCAGCTCAG
    CAAGTGCCAG ATATCACTGG TCCTTCAAGT AAACAGGCAA AAGTTACCTA TAGCTGCATT
    TATTGCAACA AACAGTTATT TTCCAGCCTT GCAGTTTTGC AGATACACCT GAAAACTATG
    CATTTAGATA AACCTGAACA AGCCCATATC TGTCAGTATT GTTTGGAGGT ATTGCCATCT
    CTCTACAATC TGAACGAACA TCTTAAGCAA GTCCACGAAG CTCCGGATCC AGGGCTGATT
    GTTTCCACTA TGCCTGCCAT GGTGTACCAG TGCAACTTCT GCTCTGAAGT GTTCAATGAC
    CTCAACACGC TTCAAGAACA TATCCGATGT TCTCATGGAT TTGCCAACCC TGCTGCTAAG
    GACAGTAATG CGTTCTTTTG CCCCCATTGC TACATGGGGT TTCTTACAGA TTCTTCTCTG
    GAAGAGCATA TTAGACAAGT CCATTGTGAT CTTAGCAGTT CCCGTTTTGG TTCCCCTGTG
    CTCGGAACCC CAAAAGATCC AGTAGTGGAA GTGTATTCTT GTTCTTACTG TACAAATTCT
    CCAATATTTA ATAGCGTTCT TAAACTGAAC AAGCATATCA AGGAGAACCA TAAGAACATT
    CCTTTGGCAC TGAATTACAT CCACAATGGA AAAAAATCCA GAGCCATGAG TCCCTTATCT
    CCTGTAACGA TTGAGCAGAC CTCTTTAAAA ATGATGCAGG CGGTTGGAGG TGCTCCTCCT
    CGTCCTGCAG GTGAATATAT TTGTAATCAA TGTGGTGCTA AGTATACTTC TTTGGACGGT
    TTTCAGACTC ATTTAAAAAC ACATCTTGAT ACTGTCCTGC CAAAACTGAC CTGCCCTCAG
    TGCAACAAGG AGTTTCCAAA TCAGGAGTCT CTGCTGAAGC ATGTTACCAT TCATTTTATG
    ATCACTTCAA CCTACTACAT CTGTGAAAGC TGTGACAAGC AGTTTACTTC TGTGGATGAC
    TTGCAGAAGC ACCTACTAGA CATGCATACA TTTGTATTCT TCCGCTGCAC CTTGTGCCAA
    GAGGTTTTTG ACTCCAAAGT CTCCATCCAA CTACACTTGG CTGTGAAGCA CAGCAATGAA
    AAGAAGGTAT ATAGGTGTAC GTCTTGCAAC TGGGATTTCC GCAATGAGAC TGACCTACAG
    CTTCACGTTA AACACAACCA CTTGGAGAAC CAAGGCAAAG TGCACAAGTG CATCTTCTGT
    GGTGAGTCCT TTGGTACAGA GGTGGAGCTG CAGTGCCATA TTACTACGCA CAGCAAGAAG
    TACAACTGTA AGTTCTGCAG CAAAGCTTTC CATGCCATCA TCCTGCTGGA AAAGCATTTA
    AGGGAAAAAC ATTGTGTTTT TGAAACAAAA ACTCCAAATT GTGGAACGAA TGGGGCATCT
    GAACAGGTTC AGAAGGAAGA AGTGGAGCTC CAGACCTTGT TGACAAATAG CCAGGAGTCC
    CATAACAGCC ATGATGGCAG CGAAGAGGAT GTGGACACAT CTGAGCCTAT GTATGGCTGT
    GACATTTGTG GAGCGGCTTA CACCATGGAG ACTCTCCTGC AAAACCACCA GCTTCGAGAC
    CACAACATTC GACCTGGAGA AAGTGCCATA GTGAAGAAAA AGGCTGAACT CATTAAAGGG
    AATTACAAGT GTAATGTGTG TTCTCGAACA TTCTTCTCTG AAAATGGGCT CCGAGAACAC
    ATGCAGACAC ACTTGGGACC AGTGAAACAT TACATGTGCC CAATCTGTGG CGAGCGGTTT
    CCATCTCTTC TGACTCTTAC TGAACATAAA GTCACACATA GTAAGAGCCT GGACACCGGA
    AACTGCAGGA TTTGCAAGAT GCCTCTCCAG AGTGAGGAGG AATTTTTAGA GCATTGCCAG
    ATGCACCCTG ACTTGAGGAA CTCTTTGACA GGGTTCCGCT GTGTGGTGTG CATGCAGACT
    GTGACCTCCA CGTTGGAACT GAAAATCCAC GGCACATTCC ACATGCAGAA AACTGGAAAT
    GGTTCTGCCG TGCAGTCCAC GGGGCGCGCA CAGCATCTCC AGAAACTGTA CAAGTGTGCT
    TCTTGCCTGA AGGAATTTCG TTCAAAACAG GATTTAGTGA AACTTGACAT CAATGGTCTG
    CCATATGGTC TGTGTGCTAG CTGTGTTAAT CTCAGTAAAA GTGGTAGTCC AAGTGTCAAC
    ATCCCATCAA GCAGTAACAG ACAAGGCATG GGCCAAAATG AGAACTTGAG TTCCATTGAG
    AACAAAAGCA AGGCAGGGGG ACTGAAGACT CGATGTTCTA GTTGCAATGT TAAATTTGAA
    TCAGAAAGTG AACTCCAAAA CCATATTCAG TCAATCCACA GAGAGCTTGT TCCAGACAGC
    AACAGTACAC AGCTGAAAAC ACCACAAGTA TCTCCAATGC CCAGAATCAG CCCCTCCCAA
    ACTGAAGAGA AGACCTACCA GTGCATCAAA TGTCAGATGG TTTTCTACAA TGAATGGGAT
    ATCCAAGTTC ATGTTGCAAA CCACATGATT GATGAAGGAC TGAACCATGA ATGCAAGCTC
    TGCAATCAGA CATTTGACTC TCCTGCCAAA CTTCAGTGCC ACCTGATAGA GCACAGCTTT
    GAAGGCATGG GGGGCACCTT CAAATGTCCA GTGTGCTTTA CAGTGTTTGT CCAAGCAAAC
    AAGTTGCAGC AGCACATTTT CTCAGCCCAT GGACAAGAGG ACAAGATCTA TGACTGCACA
    CAGTGTCCAC AGAAGTTCTT CTTCCAAACA GAGCTGCAGA ATCATACGAT GACCCAGCAT
    AGCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32511
    Clone ID Related Accession (Same CDS sequence) XM_015277987.2 , XM_015277987.1
    Accession Version XM_015277987.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4089bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product zinc finger protein 521 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277987.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCTTTGG GGGCCTTGCT TCCATTTTCC ATTATTATGT GGACTACCGG AGCGACAGCG 
    CAGTCCAAGA CCTTGCAGCT GTTTGCTTTT AGGATGTCTC GCCGCAAGCA AGCTAAACCG
    AGATCACTCA AAGTAGAAGA AAATGAAACA GAGGACCTGC AGGCAGGTGT GGGACACACA
    GCCGCGCAGA CTGACCCTAA CTGTAAACTA GAAGACAAGA CAGAAGATGG AGAAGTGTTA
    GACTGTAAGA AAAGGCCGGA TGAAGGGGAG GAGTTGGAAG AAGAAGCCGT GCACAGCTGT
    GACAGCTGCC TCCAGGTGTT TGAGTCGCTG AGTGATATCA CAGAACACAA GATTAATCAA
    TGTCAACTGA CAGATGGAGT GGATGTTGAG GATGACCCCA CTTGCTCATG GCCAGCATCA
    TCGCCTTCCA GCAAGGACCA GACTTCCCCA AGCCATGGAG AAGGTTGTGA TTTTGGTGAG
    GAGGAAGGAG GCCCAGGGTT GCCCTATCAG TGCCAGTTTT GTGACAAATC GTTCAGCCGC
    CTCAGCTACT TAAAGCACCA CGAGCAAAGT CACAGTGACA AACTCCCTTT CAAATGCACT
    TATTGCAGTC GTCTCTTCAA ACACAAACGA AGCAGGGACC GCCACATAAA GCTTCATACG
    GGAGACAAGA AGTATCACTG CAGTGAGTGT GATGCAGCAT TCTCAAGGAG CGATCATCTT
    AAAATTCACT TAAAGACTCA TACGTCCAAC AAGCCATATA AATGTGCCAT TTGTAGACGT
    GGATTCCTGT CATCCAGTTC GCTGCACGGC CACATGCAGG TTCACGAGAG GAACAAGGAT
    GGCTCTCAGT CTGCTTCCCG GATGGAGGAC TGGAAAATGA AGGACACTCA GAAGTGCAGT
    CAGTGTGAAG AAGGCTTTGA TTTTCCTGAA GATCTTCAGA AGCACATTGC GGAATGTCAC
    CCTGAGTGTT CTCCTAATGA AGACCGATCT GCTCTCCAGT GTGTTTACTG TCACGAACTC
    TTTGTAGAGG AAACGTCTCT GGTGAATCAC ATGGAGCAGG CTCATAATGG TGAGAAGAAG
    AATTCATGCA GCATTTGCTC TGAGAACTTT CACACTGTGG AAGAGCTGTA CAGCCACATG
    GACAGTCACC AGCAGCCAGA ATCATGCAAC CACAGCAACA GCCCATCATT GGTAACTGTT
    GGCTACACCT CAGTCTCAAG TACCACTCCA GATTCCAATC TCTCTGTGGA TAGTTCAACA
    ATGGTGGAAA CGGCTCCCCC TATACCAAAA GGCCGTGGAA GAAAGCGGGC AGCTCAGCAA
    GTGCCAGATA TCACTGGTCC TTCAAGTAAA CAGGCAAAAG TTACCTATAG CTGCATTTAT
    TGCAACAAAC AGTTATTTTC CAGCCTTGCA GTTTTGCAGA TACACCTGAA AACTATGCAT
    TTAGATAAAC CTGAACAAGC CCATATCTGT CAGTATTGTT TGGAGGTATT GCCATCTCTC
    TACAATCTGA ACGAACATCT TAAGCAAGTC CACGAAGCTC CGGATCCAGG GCTGATTGTT
    TCCACTATGC CTGCCATGGT GTACCAGTGC AACTTCTGCT CTGAAGTGTT CAATGACCTC
    AACACGCTTC AAGAACATAT CCGATGTTCT CATGGATTTG CCAACCCTGC TGCTAAGGAC
    AGTAATGCGT TCTTTTGCCC CCATTGCTAC ATGGGGTTTC TTACAGATTC TTCTCTGGAA
    GAGCATATTA GACAAGTCCA TTGTGATCTT AGCAGTTCCC GTTTTGGTTC CCCTGTGCTC
    GGAACCCCAA AAGATCCAGT AGTGGAAGTG TATTCTTGTT CTTACTGTAC AAATTCTCCA
    ATATTTAATA GCGTTCTTAA ACTGAACAAG CATATCAAGG AGAACCATAA GAACATTCCT
    TTGGCACTGA ATTACATCCA CAATGGAAAA AAATCCAGAG CCATGAGTCC CTTATCTCCT
    GTAACGATTG AGCAGACCTC TTTAAAAATG ATGCAGGCGG TTGGAGGTGC TCCTCCTCGT
    CCTGCAGGTG AATATATTTG TAATCAATGT GGTGCTAAGT ATACTTCTTT GGACGGTTTT
    CAGACTCATT TAAAAACACA TCTTGATACT GTCCTGCCAA AACTGACCTG CCCTCAGTGC
    AACAAGGAGT TTCCAAATCA GGAGTCTCTG CTGAAGCATG TTACCATTCA TTTTATGATC
    ACTTCAACCT ACTACATCTG TGAAAGCTGT GACAAGCAGT TTACTTCTGT GGATGACTTG
    CAGAAGCACC TACTAGACAT GCATACATTT GTATTCTTCC GCTGCACCTT GTGCCAAGAG
    GTTTTTGACT CCAAAGTCTC CATCCAACTA CACTTGGCTG TGAAGCACAG CAATGAAAAG
    AAGGTATATA GGTGTACGTC TTGCAACTGG GATTTCCGCA ATGAGACTGA CCTACAGCTT
    CACGTTAAAC ACAACCACTT GGAGAACCAA GGCAAAGTGC ACAAGTGCAT CTTCTGTGGT
    GAGTCCTTTG GTACAGAGGT GGAGCTGCAG TGCCATATTA CTACGCACAG CAAGAAGTAC
    AACTGTAAGT TCTGCAGCAA AGCTTTCCAT GCCATCATCC TGCTGGAAAA GCATTTAAGG
    GAAAAACATT GTGTTTTTGA AACAAAAACT CCAAATTGTG GAACGAATGG GGCATCTGAA
    CAGGTTCAGA AGGAAGAAGT GGAGCTCCAG ACCTTGTTGA CAAATAGCCA GGAGTCCCAT
    AACAGCCATG ATGGCAGCGA AGAGGATGTG GACACATCTG AGCCTATGTA TGGCTGTGAC
    ATTTGTGGAG CGGCTTACAC CATGGAGACT CTCCTGCAAA ACCACCAGCT TCGAGACCAC
    AACATTCGAC CTGGAGAAAG TGCCATAGTG AAGAAAAAGG CTGAACTCAT TAAAGGGAAT
    TACAAGTGTA ATGTGTGTTC TCGAACATTC TTCTCTGAAA ATGGGCTCCG AGAACACATG
    CAGACACACT TGGGACCAGT GAAACATTAC ATGTGCCCAA TCTGTGGCGA GCGGTTTCCA
    TCTCTTCTGA CTCTTACTGA ACATAAAGTC ACACATAGTA AGAGCCTGGA CACCGGAAAC
    TGCAGGATTT GCAAGATGCC TCTCCAGAGT GAGGAGGAAT TTTTAGAGCA TTGCCAGATG
    CACCCTGACT TGAGGAACTC TTTGACAGGG TTCCGCTGTG TGGTGTGCAT GCAGACTGTG
    ACCTCCACGT TGGAACTGAA AATCCACGGC ACATTCCACA TGCAGAAAAC TGGAAATGGT
    TCTGCCGTGC AGTCCACGGG GCGCGCACAG CATCTCCAGA AACTGTACAA GTGTGCTTCT
    TGCCTGAAGG AATTTCGTTC AAAACAGGAT TTAGTGAAAC TTGACATCAA TGGTCTGCCA
    TATGGTCTGT GTGCTAGCTG TGTTAATCTC AGTAAAAGTG GTAGTCCAAG TGTCAACATC
    CCATCAAGCA GTAACAGACA AGGCATGGGC CAAAATGAGA ACTTGAGTTC CATTGAGAAC
    AAAAGCAAGG CAGGGGGACT GAAGACTCGA TGTTCTAGTT GCAATGTTAA ATTTGAATCA
    GAAAGTGAAC TCCAAAACCA TATTCAGTCA ATCCACAGAG AGCTTGTTCC AGACAGCAAC
    AGTACACAGC TGAAAACACC ACAAGTATCT CCAATGCCCA GAATCAGCCC CTCCCAAACT
    GAAGAGAAGA AGACCTACCA GTGCATCAAA TGTCAGATGG TTTTCTACAA TGAATGGGAT
    ATCCAAGTTC ATGTTGCAAA CCACATGATT GATGAAGGAC TGAACCATGA ATGCAAGCTC
    TGCAATCAGA CATTTGACTC TCCTGCCAAA CTTCAGTGCC ACCTGATAGA GCACAGCTTT
    GAAGGCATGG GGGGCACCTT CAAATGTCCA GTGTGCTTTA CAGTGTTTGT CCAAGCAAAC
    AAGTTGCAGC AGCACATTTT CTCAGCCCAT GGACAAGAGG ACAAGATCTA TGACTGCACA
    CAGTGTCCAC AGAAGTTCTT CTTCCAAACA GAGCTGCAGA ATCATACGAT GACCCAGCAT
    AGCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133473.1
    CDS47..4135
    Translation

    Target ORF information:

    RefSeq Version XM_015277987.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277987.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCTTTGG GGGCCTTGCT TCCATTTTCC ATTATTATGT GGACTACCGG AGCGACAGCG 
    CAGTCCAAGA CCTTGCAGCT GTTTGCTTTT AGGATGTCTC GCCGCAAGCA AGCTAAACCG
    AGATCACTCA AAGTAGAAGA AAATGAAACA GAGGACCTGC AGGCAGGTGT GGGACACACA
    GCCGCGCAGA CTGACCCTAA CTGTAAACTA GAAGACAAGA CAGAAGATGG AGAAGTGTTA
    GACTGTAAGA AAAGGCCGGA TGAAGGGGAG GAGTTGGAAG AAGAAGCCGT GCACAGCTGT
    GACAGCTGCC TCCAGGTGTT TGAGTCGCTG AGTGATATCA CAGAACACAA GATTAATCAA
    TGTCAACTGA CAGATGGAGT GGATGTTGAG GATGACCCCA CTTGCTCATG GCCAGCATCA
    TCGCCTTCCA GCAAGGACCA GACTTCCCCA AGCCATGGAG AAGGTTGTGA TTTTGGTGAG
    GAGGAAGGAG GCCCAGGGTT GCCCTATCAG TGCCAGTTTT GTGACAAATC GTTCAGCCGC
    CTCAGCTACT TAAAGCACCA CGAGCAAAGT CACAGTGACA AACTCCCTTT CAAATGCACT
    TATTGCAGTC GTCTCTTCAA ACACAAACGA AGCAGGGACC GCCACATAAA GCTTCATACG
    GGAGACAAGA AGTATCACTG CAGTGAGTGT GATGCAGCAT TCTCAAGGAG CGATCATCTT
    AAAATTCACT TAAAGACTCA TACGTCCAAC AAGCCATATA AATGTGCCAT TTGTAGACGT
    GGATTCCTGT CATCCAGTTC GCTGCACGGC CACATGCAGG TTCACGAGAG GAACAAGGAT
    GGCTCTCAGT CTGCTTCCCG GATGGAGGAC TGGAAAATGA AGGACACTCA GAAGTGCAGT
    CAGTGTGAAG AAGGCTTTGA TTTTCCTGAA GATCTTCAGA AGCACATTGC GGAATGTCAC
    CCTGAGTGTT CTCCTAATGA AGACCGATCT GCTCTCCAGT GTGTTTACTG TCACGAACTC
    TTTGTAGAGG AAACGTCTCT GGTGAATCAC ATGGAGCAGG CTCATAATGG TGAGAAGAAG
    AATTCATGCA GCATTTGCTC TGAGAACTTT CACACTGTGG AAGAGCTGTA CAGCCACATG
    GACAGTCACC AGCAGCCAGA ATCATGCAAC CACAGCAACA GCCCATCATT GGTAACTGTT
    GGCTACACCT CAGTCTCAAG TACCACTCCA GATTCCAATC TCTCTGTGGA TAGTTCAACA
    ATGGTGGAAA CGGCTCCCCC TATACCAAAA GGCCGTGGAA GAAAGCGGGC AGCTCAGCAA
    GTGCCAGATA TCACTGGTCC TTCAAGTAAA CAGGCAAAAG TTACCTATAG CTGCATTTAT
    TGCAACAAAC AGTTATTTTC CAGCCTTGCA GTTTTGCAGA TACACCTGAA AACTATGCAT
    TTAGATAAAC CTGAACAAGC CCATATCTGT CAGTATTGTT TGGAGGTATT GCCATCTCTC
    TACAATCTGA ACGAACATCT TAAGCAAGTC CACGAAGCTC CGGATCCAGG GCTGATTGTT
    TCCACTATGC CTGCCATGGT GTACCAGTGC AACTTCTGCT CTGAAGTGTT CAATGACCTC
    AACACGCTTC AAGAACATAT CCGATGTTCT CATGGATTTG CCAACCCTGC TGCTAAGGAC
    AGTAATGCGT TCTTTTGCCC CCATTGCTAC ATGGGGTTTC TTACAGATTC TTCTCTGGAA
    GAGCATATTA GACAAGTCCA TTGTGATCTT AGCAGTTCCC GTTTTGGTTC CCCTGTGCTC
    GGAACCCCAA AAGATCCAGT AGTGGAAGTG TATTCTTGTT CTTACTGTAC AAATTCTCCA
    ATATTTAATA GCGTTCTTAA ACTGAACAAG CATATCAAGG AGAACCATAA GAACATTCCT
    TTGGCACTGA ATTACATCCA CAATGGAAAA AAATCCAGAG CCATGAGTCC CTTATCTCCT
    GTAACGATTG AGCAGACCTC TTTAAAAATG ATGCAGGCGG TTGGAGGTGC TCCTCCTCGT
    CCTGCAGGTG AATATATTTG TAATCAATGT GGTGCTAAGT ATACTTCTTT GGACGGTTTT
    CAGACTCATT TAAAAACACA TCTTGATACT GTCCTGCCAA AACTGACCTG CCCTCAGTGC
    AACAAGGAGT TTCCAAATCA GGAGTCTCTG CTGAAGCATG TTACCATTCA TTTTATGATC
    ACTTCAACCT ACTACATCTG TGAAAGCTGT GACAAGCAGT TTACTTCTGT GGATGACTTG
    CAGAAGCACC TACTAGACAT GCATACATTT GTATTCTTCC GCTGCACCTT GTGCCAAGAG
    GTTTTTGACT CCAAAGTCTC CATCCAACTA CACTTGGCTG TGAAGCACAG CAATGAAAAG
    AAGGTATATA GGTGTACGTC TTGCAACTGG GATTTCCGCA ATGAGACTGA CCTACAGCTT
    CACGTTAAAC ACAACCACTT GGAGAACCAA GGCAAAGTGC ACAAGTGCAT CTTCTGTGGT
    GAGTCCTTTG GTACAGAGGT GGAGCTGCAG TGCCATATTA CTACGCACAG CAAGAAGTAC
    AACTGTAAGT TCTGCAGCAA AGCTTTCCAT GCCATCATCC TGCTGGAAAA GCATTTAAGG
    GAAAAACATT GTGTTTTTGA AACAAAAACT CCAAATTGTG GAACGAATGG GGCATCTGAA
    CAGGTTCAGA AGGAAGAAGT GGAGCTCCAG ACCTTGTTGA CAAATAGCCA GGAGTCCCAT
    AACAGCCATG ATGGCAGCGA AGAGGATGTG GACACATCTG AGCCTATGTA TGGCTGTGAC
    ATTTGTGGAG CGGCTTACAC CATGGAGACT CTCCTGCAAA ACCACCAGCT TCGAGACCAC
    AACATTCGAC CTGGAGAAAG TGCCATAGTG AAGAAAAAGG CTGAACTCAT TAAAGGGAAT
    TACAAGTGTA ATGTGTGTTC TCGAACATTC TTCTCTGAAA ATGGGCTCCG AGAACACATG
    CAGACACACT TGGGACCAGT GAAACATTAC ATGTGCCCAA TCTGTGGCGA GCGGTTTCCA
    TCTCTTCTGA CTCTTACTGA ACATAAAGTC ACACATAGTA AGAGCCTGGA CACCGGAAAC
    TGCAGGATTT GCAAGATGCC TCTCCAGAGT GAGGAGGAAT TTTTAGAGCA TTGCCAGATG
    CACCCTGACT TGAGGAACTC TTTGACAGGG TTCCGCTGTG TGGTGTGCAT GCAGACTGTG
    ACCTCCACGT TGGAACTGAA AATCCACGGC ACATTCCACA TGCAGAAAAC TGGAAATGGT
    TCTGCCGTGC AGTCCACGGG GCGCGCACAG CATCTCCAGA AACTGTACAA GTGTGCTTCT
    TGCCTGAAGG AATTTCGTTC AAAACAGGAT TTAGTGAAAC TTGACATCAA TGGTCTGCCA
    TATGGTCTGT GTGCTAGCTG TGTTAATCTC AGTAAAAGTG GTAGTCCAAG TGTCAACATC
    CCATCAAGCA GTAACAGACA AGGCATGGGC CAAAATGAGA ACTTGAGTTC CATTGAGAAC
    AAAAGCAAGG CAGGGGGACT GAAGACTCGA TGTTCTAGTT GCAATGTTAA ATTTGAATCA
    GAAAGTGAAC TCCAAAACCA TATTCAGTCA ATCCACAGAG AGCTTGTTCC AGACAGCAAC
    AGTACACAGC TGAAAACACC ACAAGTATCT CCAATGCCCA GAATCAGCCC CTCCCAAACT
    GAAGAGAAGA AGACCTACCA GTGCATCAAA TGTCAGATGG TTTTCTACAA TGAATGGGAT
    ATCCAAGTTC ATGTTGCAAA CCACATGATT GATGAAGGAC TGAACCATGA ATGCAAGCTC
    TGCAATCAGA CATTTGACTC TCCTGCCAAA CTTCAGTGCC ACCTGATAGA GCACAGCTTT
    GAAGGCATGG GGGGCACCTT CAAATGTCCA GTGTGCTTTA CAGTGTTTGT CCAAGCAAAC
    AAGTTGCAGC AGCACATTTT CTCAGCCCAT GGACAAGAGG ACAAGATCTA TGACTGCACA
    CAGTGTCCAC AGAAGTTCTT CTTCCAAACA GAGCTGCAGA ATCATACGAT GACCCAGCAT
    AGCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32511
    Clone ID Related Accession (Same CDS sequence) XM_015277987.2 , XM_015277987.1
    Accession Version XM_015277987.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4089bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product zinc finger protein 521 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCTTTGG GGGCCTTGCT TCCATTTTCC ATTATTATGT GGACTACCGG AGCGACAGCG 
    CAGTCCAAGA CCTTGCAGCT GTTTGCTTTT AGGATGTCTC GCCGCAAGCA AGCTAAACCG
    AGATCACTCA AAGTAGAAGA AAATGAAACA GAGGACCTGC AGGCAGGTGT GGGACACACA
    GCCGCGCAGA CTGACCCTAA CTGTAAACTA GAAGACAAGA CAGAAGATGG AGAAGTGTTA
    GACTGTAAGA AAAGGCCGGA TGAAGGGGAG GAGTTGGAAG AAGAAGCCGT GCACAGCTGT
    GACAGCTGCC TCCAGGTGTT TGAGTCGCTG AGTGATATCA CAGAACACAA GATTAATCAA
    TGTCAACTGA CAGATGGAGT GGATGTTGAG GATGACCCCA CTTGCTCATG GCCAGCATCA
    TCGCCTTCCA GCAAGGACCA GACTTCCCCA AGCCATGGAG AAGGTTGTGA TTTTGGTGAG
    GAGGAAGGAG GCCCAGGGTT GCCCTATCAG TGCCAGTTTT GTGACAAATC GTTCAGCCGC
    CTCAGCTACT TAAAGCACCA CGAGCAAAGT CACAGTGACA AACTCCCTTT CAAATGCACT
    TATTGCAGTC GTCTCTTCAA ACACAAACGA AGCAGGGACC GCCACATAAA GCTTCATACG
    GGAGACAAGA AGTATCACTG CAGTGAGTGT GATGCAGCAT TCTCAAGGAG CGATCATCTT
    AAAATTCACT TAAAGACTCA TACGTCCAAC AAGCCATATA AATGTGCCAT TTGTAGACGT
    GGATTCCTGT CATCCAGTTC GCTGCACGGC CACATGCAGG TTCACGAGAG GAACAAGGAT
    GGCTCTCAGT CTGCTTCCCG GATGGAGGAC TGGAAAATGA AGGACACTCA GAAGTGCAGT
    CAGTGTGAAG AAGGCTTTGA TTTTCCTGAA GATCTTCAGA AGCACATTGC GGAATGTCAC
    CCTGAGTGTT CTCCTAATGA AGACCGATCT GCTCTCCAGT GTGTTTACTG TCACGAACTC
    TTTGTAGAGG AAACGTCTCT GGTGAATCAC ATGGAGCAGG CTCATAATGG TGAGAAGAAG
    AATTCATGCA GCATTTGCTC TGAGAACTTT CACACTGTGG AAGAGCTGTA CAGCCACATG
    GACAGTCACC AGCAGCCAGA ATCATGCAAC CACAGCAACA GCCCATCATT GGTAACTGTT
    GGCTACACCT CAGTCTCAAG TACCACTCCA GATTCCAATC TCTCTGTGGA TAGTTCAACA
    ATGGTGGAAA CGGCTCCCCC TATACCAAAA GGCCGTGGAA GAAAGCGGGC AGCTCAGCAA
    GTGCCAGATA TCACTGGTCC TTCAAGTAAA CAGGCAAAAG TTACCTATAG CTGCATTTAT
    TGCAACAAAC AGTTATTTTC CAGCCTTGCA GTTTTGCAGA TACACCTGAA AACTATGCAT
    TTAGATAAAC CTGAACAAGC CCATATCTGT CAGTATTGTT TGGAGGTATT GCCATCTCTC
    TACAATCTGA ACGAACATCT TAAGCAAGTC CACGAAGCTC CGGATCCAGG GCTGATTGTT
    TCCACTATGC CTGCCATGGT GTACCAGTGC AACTTCTGCT CTGAAGTGTT CAATGACCTC
    AACACGCTTC AAGAACATAT CCGATGTTCT CATGGATTTG CCAACCCTGC TGCTAAGGAC
    AGTAATGCGT TCTTTTGCCC CCATTGCTAC ATGGGGTTTC TTACAGATTC TTCTCTGGAA
    GAGCATATTA GACAAGTCCA TTGTGATCTT AGCAGTTCCC GTTTTGGTTC CCCTGTGCTC
    GGAACCCCAA AAGATCCAGT AGTGGAAGTG TATTCTTGTT CTTACTGTAC AAATTCTCCA
    ATATTTAATA GCGTTCTTAA ACTGAACAAG CATATCAAGG AGAACCATAA GAACATTCCT
    TTGGCACTGA ATTACATCCA CAATGGAAAA AAATCCAGAG CCATGAGTCC CTTATCTCCT
    GTAACGATTG AGCAGACCTC TTTAAAAATG ATGCAGGCGG TTGGAGGTGC TCCTCCTCGT
    CCTGCAGGTG AATATATTTG TAATCAATGT GGTGCTAAGT ATACTTCTTT GGACGGTTTT
    CAGACTCATT TAAAAACACA TCTTGATACT GTCCTGCCAA AACTGACCTG CCCTCAGTGC
    AACAAGGAGT TTCCAAATCA GGAGTCTCTG CTGAAGCATG TTACCATTCA TTTTATGATC
    ACTTCAACCT ACTACATCTG TGAAAGCTGT GACAAGCAGT TTACTTCTGT GGATGACTTG
    CAGAAGCACC TACTAGACAT GCATACATTT GTATTCTTCC GCTGCACCTT GTGCCAAGAG
    GTTTTTGACT CCAAAGTCTC CATCCAACTA CACTTGGCTG TGAAGCACAG CAATGAAAAG
    AAGGTATATA GGTGTACGTC TTGCAACTGG GATTTCCGCA ATGAGACTGA CCTACAGCTT
    CACGTTAAAC ACAACCACTT GGAGAACCAA GGCAAAGTGC ACAAGTGCAT CTTCTGTGGT
    GAGTCCTTTG GTACAGAGGT GGAGCTGCAG TGCCATATTA CTACGCACAG CAAGAAGTAC
    AACTGTAAGT TCTGCAGCAA AGCTTTCCAT GCCATCATCC TGCTGGAAAA GCATTTAAGG
    GAAAAACATT GTGTTTTTGA AACAAAAACT CCAAATTGTG GAACGAATGG GGCATCTGAA
    CAGGTTCAGA AGGAAGAAGT GGAGCTCCAG ACCTTGTTGA CAAATAGCCA GGAGTCCCAT
    AACAGCCATG ATGGCAGCGA AGAGGATGTG GACACATCTG AGCCTATGTA TGGCTGTGAC
    ATTTGTGGAG CGGCTTACAC CATGGAGACT CTCCTGCAAA ACCACCAGCT TCGAGACCAC
    AACATTCGAC CTGGAGAAAG TGCCATAGTG AAGAAAAAGG CTGAACTCAT TAAAGGGAAT
    TACAAGTGTA ATGTGTGTTC TCGAACATTC TTCTCTGAAA ATGGGCTCCG AGAACACATG
    CAGACACACT TGGGACCAGT GAAACATTAC ATGTGCCCAA TCTGTGGCGA GCGGTTTCCA
    TCTCTTCTGA CTCTTACTGA ACATAAAGTC ACACATAGTA AGAGCCTGGA CACCGGAAAC
    TGCAGGATTT GCAAGATGCC TCTCCAGAGT GAGGAGGAAT TTTTAGAGCA TTGCCAGATG
    CACCCTGACT TGAGGAACTC TTTGACAGGG TTCCGCTGTG TGGTGTGCAT GCAGACTGTG
    ACCTCCACGT TGGAACTGAA AATCCACGGC ACATTCCACA TGCAGAAAAC TGGAAATGGT
    TCTGCCGTGC AGTCCACGGG GCGCGCACAG CATCTCCAGA AACTGTACAA GTGTGCTTCT
    TGCCTGAAGG AATTTCGTTC AAAACAGGAT TTAGTGAAAC TTGACATCAA TGGTCTGCCA
    TATGGTCTGT GTGCTAGCTG TGTTAATCTC AGTAAAAGTG GTAGTCCAAG TGTCAACATC
    CCATCAAGCA GTAACAGACA AGGCATGGGC CAAAATGAGA ACTTGAGTTC CATTGAGAAC
    AAAAGCAAGG CAGGGGGACT GAAGACTCGA TGTTCTAGTT GCAATGTTAA ATTTGAATCA
    GAAAGTGAAC TCCAAAACCA TATTCAGTCA ATCCACAGAG AGCTTGTTCC AGACAGCAAC
    AGTACACAGC TGAAAACACC ACAAGTATCT CCAATGCCCA GAATCAGCCC CTCCCAAACT
    GAAGAGAAGA AGACCTACCA GTGCATCAAA TGTCAGATGG TTTTCTACAA TGAATGGGAT
    ATCCAAGTTC ATGTTGCAAA CCACATGATT GATGAAGGAC TGAACCATGA ATGCAAGCTC
    TGCAATCAGA CATTTGACTC TCCTGCCAAA CTTCAGTGCC ACCTGATAGA GCACAGCTTT
    GAAGGCATGG GGGGCACCTT CAAATGTCCA GTGTGCTTTA CAGTGTTTGT CCAAGCAAAC
    AAGTTGCAGC AGCACATTTT CTCAGCCCAT GGACAAGAGG ACAAGATCTA TGACTGCACA
    CAGTGTCCAC AGAAGTTCTT CTTCCAAACA GAGCTGCAGA ATCATACGAT GACCCAGCAT
    AGCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133473.1
    CDS5..4093
    Translation

    Target ORF information:

    RefSeq Version XM_015277987.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277987.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCTTTGG GGGCCTTGCT TCCATTTTCC ATTATTATGT GGACTACCGG AGCGACAGCG 
    CAGTCCAAGA CCTTGCAGCT GTTTGCTTTT AGGATGTCTC GCCGCAAGCA AGCTAAACCG
    AGATCACTCA AAGTAGAAGA AAATGAAACA GAGGACCTGC AGGCAGGTGT GGGACACACA
    GCCGCGCAGA CTGACCCTAA CTGTAAACTA GAAGACAAGA CAGAAGATGG AGAAGTGTTA
    GACTGTAAGA AAAGGCCGGA TGAAGGGGAG GAGTTGGAAG AAGAAGCCGT GCACAGCTGT
    GACAGCTGCC TCCAGGTGTT TGAGTCGCTG AGTGATATCA CAGAACACAA GATTAATCAA
    TGTCAACTGA CAGATGGAGT GGATGTTGAG GATGACCCCA CTTGCTCATG GCCAGCATCA
    TCGCCTTCCA GCAAGGACCA GACTTCCCCA AGCCATGGAG AAGGTTGTGA TTTTGGTGAG
    GAGGAAGGAG GCCCAGGGTT GCCCTATCAG TGCCAGTTTT GTGACAAATC GTTCAGCCGC
    CTCAGCTACT TAAAGCACCA CGAGCAAAGT CACAGTGACA AACTCCCTTT CAAATGCACT
    TATTGCAGTC GTCTCTTCAA ACACAAACGA AGCAGGGACC GCCACATAAA GCTTCATACG
    GGAGACAAGA AGTATCACTG CAGTGAGTGT GATGCAGCAT TCTCAAGGAG CGATCATCTT
    AAAATTCACT TAAAGACTCA TACGTCCAAC AAGCCATATA AATGTGCCAT TTGTAGACGT
    GGATTCCTGT CATCCAGTTC GCTGCACGGC CACATGCAGG TTCACGAGAG GAACAAGGAT
    GGCTCTCAGT CTGCTTCCCG GATGGAGGAC TGGAAAATGA AGGACACTCA GAAGTGCAGT
    CAGTGTGAAG AAGGCTTTGA TTTTCCTGAA GATCTTCAGA AGCACATTGC GGAATGTCAC
    CCTGAGTGTT CTCCTAATGA AGACCGATCT GCTCTCCAGT GTGTTTACTG TCACGAACTC
    TTTGTAGAGG AAACGTCTCT GGTGAATCAC ATGGAGCAGG CTCATAATGG TGAGAAGAAG
    AATTCATGCA GCATTTGCTC TGAGAACTTT CACACTGTGG AAGAGCTGTA CAGCCACATG
    GACAGTCACC AGCAGCCAGA ATCATGCAAC CACAGCAACA GCCCATCATT GGTAACTGTT
    GGCTACACCT CAGTCTCAAG TACCACTCCA GATTCCAATC TCTCTGTGGA TAGTTCAACA
    ATGGTGGAAA CGGCTCCCCC TATACCAAAA GGCCGTGGAA GAAAGCGGGC AGCTCAGCAA
    GTGCCAGATA TCACTGGTCC TTCAAGTAAA CAGGCAAAAG TTACCTATAG CTGCATTTAT
    TGCAACAAAC AGTTATTTTC CAGCCTTGCA GTTTTGCAGA TACACCTGAA AACTATGCAT
    TTAGATAAAC CTGAACAAGC CCATATCTGT CAGTATTGTT TGGAGGTATT GCCATCTCTC
    TACAATCTGA ACGAACATCT TAAGCAAGTC CACGAAGCTC CGGATCCAGG GCTGATTGTT
    TCCACTATGC CTGCCATGGT GTACCAGTGC AACTTCTGCT CTGAAGTGTT CAATGACCTC
    AACACGCTTC AAGAACATAT CCGATGTTCT CATGGATTTG CCAACCCTGC TGCTAAGGAC
    AGTAATGCGT TCTTTTGCCC CCATTGCTAC ATGGGGTTTC TTACAGATTC TTCTCTGGAA
    GAGCATATTA GACAAGTCCA TTGTGATCTT AGCAGTTCCC GTTTTGGTTC CCCTGTGCTC
    GGAACCCCAA AAGATCCAGT AGTGGAAGTG TATTCTTGTT CTTACTGTAC AAATTCTCCA
    ATATTTAATA GCGTTCTTAA ACTGAACAAG CATATCAAGG AGAACCATAA GAACATTCCT
    TTGGCACTGA ATTACATCCA CAATGGAAAA AAATCCAGAG CCATGAGTCC CTTATCTCCT
    GTAACGATTG AGCAGACCTC TTTAAAAATG ATGCAGGCGG TTGGAGGTGC TCCTCCTCGT
    CCTGCAGGTG AATATATTTG TAATCAATGT GGTGCTAAGT ATACTTCTTT GGACGGTTTT
    CAGACTCATT TAAAAACACA TCTTGATACT GTCCTGCCAA AACTGACCTG CCCTCAGTGC
    AACAAGGAGT TTCCAAATCA GGAGTCTCTG CTGAAGCATG TTACCATTCA TTTTATGATC
    ACTTCAACCT ACTACATCTG TGAAAGCTGT GACAAGCAGT TTACTTCTGT GGATGACTTG
    CAGAAGCACC TACTAGACAT GCATACATTT GTATTCTTCC GCTGCACCTT GTGCCAAGAG
    GTTTTTGACT CCAAAGTCTC CATCCAACTA CACTTGGCTG TGAAGCACAG CAATGAAAAG
    AAGGTATATA GGTGTACGTC TTGCAACTGG GATTTCCGCA ATGAGACTGA CCTACAGCTT
    CACGTTAAAC ACAACCACTT GGAGAACCAA GGCAAAGTGC ACAAGTGCAT CTTCTGTGGT
    GAGTCCTTTG GTACAGAGGT GGAGCTGCAG TGCCATATTA CTACGCACAG CAAGAAGTAC
    AACTGTAAGT TCTGCAGCAA AGCTTTCCAT GCCATCATCC TGCTGGAAAA GCATTTAAGG
    GAAAAACATT GTGTTTTTGA AACAAAAACT CCAAATTGTG GAACGAATGG GGCATCTGAA
    CAGGTTCAGA AGGAAGAAGT GGAGCTCCAG ACCTTGTTGA CAAATAGCCA GGAGTCCCAT
    AACAGCCATG ATGGCAGCGA AGAGGATGTG GACACATCTG AGCCTATGTA TGGCTGTGAC
    ATTTGTGGAG CGGCTTACAC CATGGAGACT CTCCTGCAAA ACCACCAGCT TCGAGACCAC
    AACATTCGAC CTGGAGAAAG TGCCATAGTG AAGAAAAAGG CTGAACTCAT TAAAGGGAAT
    TACAAGTGTA ATGTGTGTTC TCGAACATTC TTCTCTGAAA ATGGGCTCCG AGAACACATG
    CAGACACACT TGGGACCAGT GAAACATTAC ATGTGCCCAA TCTGTGGCGA GCGGTTTCCA
    TCTCTTCTGA CTCTTACTGA ACATAAAGTC ACACATAGTA AGAGCCTGGA CACCGGAAAC
    TGCAGGATTT GCAAGATGCC TCTCCAGAGT GAGGAGGAAT TTTTAGAGCA TTGCCAGATG
    CACCCTGACT TGAGGAACTC TTTGACAGGG TTCCGCTGTG TGGTGTGCAT GCAGACTGTG
    ACCTCCACGT TGGAACTGAA AATCCACGGC ACATTCCACA TGCAGAAAAC TGGAAATGGT
    TCTGCCGTGC AGTCCACGGG GCGCGCACAG CATCTCCAGA AACTGTACAA GTGTGCTTCT
    TGCCTGAAGG AATTTCGTTC AAAACAGGAT TTAGTGAAAC TTGACATCAA TGGTCTGCCA
    TATGGTCTGT GTGCTAGCTG TGTTAATCTC AGTAAAAGTG GTAGTCCAAG TGTCAACATC
    CCATCAAGCA GTAACAGACA AGGCATGGGC CAAAATGAGA ACTTGAGTTC CATTGAGAAC
    AAAAGCAAGG CAGGGGGACT GAAGACTCGA TGTTCTAGTT GCAATGTTAA ATTTGAATCA
    GAAAGTGAAC TCCAAAACCA TATTCAGTCA ATCCACAGAG AGCTTGTTCC AGACAGCAAC
    AGTACACAGC TGAAAACACC ACAAGTATCT CCAATGCCCA GAATCAGCCC CTCCCAAACT
    GAAGAGAAGA AGACCTACCA GTGCATCAAA TGTCAGATGG TTTTCTACAA TGAATGGGAT
    ATCCAAGTTC ATGTTGCAAA CCACATGATT GATGAAGGAC TGAACCATGA ATGCAAGCTC
    TGCAATCAGA CATTTGACTC TCCTGCCAAA CTTCAGTGCC ACCTGATAGA GCACAGCTTT
    GAAGGCATGG GGGGCACCTT CAAATGTCCA GTGTGCTTTA CAGTGTTTGT CCAAGCAAAC
    AAGTTGCAGC AGCACATTTT CTCAGCCCAT GGACAAGAGG ACAAGATCTA TGACTGCACA
    CAGTGTCCAC AGAAGTTCTT CTTCCAAACA GAGCTGCAGA ATCATACGAT GACCCAGCAT
    AGCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32512
    Clone ID Related Accession (Same CDS sequence) XM_015277988.1 , XM_015277988.2
    Accession Version XM_015277988.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product zinc finger protein 521 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCTCGCC GCAAGCAAGC TAAACCGAGA TCACTCAAAG AAACCCAGAT TCTAGAAACA 
    ACCAATTGTA TGAAGCCTTG GGGTTGGCGG AGGAGGAAAA GAGTAGAAGA AAATGAAACA
    GAGGACCTGC AGGCAGGTGT GGGACACACA GCCGCGCAGA CTGACCCTAA CTGTAAACTA
    GAAGACAAGA CAGAAGATGG AGAAGTGTTA GACTGTAAGA AAAGGCCGGA TGAAGGGGAG
    GAGTTGGAAG AAGAAGCCGT GCACAGCTGT GACAGCTGCC TCCAGGTGTT TGAGTCGCTG
    AGTGATATCA CAGAACACAA GATTAATCAA TGTCAACTGA CAGATGGAGT GGATGTTGAG
    GATGACCCCA CTTGCTCATG GCCAGCATCA TCGCCTTCCA GCAAGGACCA GACTTCCCCA
    AGCCATGGAG AAGGTTGTGA TTTTGGTGAG GAGGAAGGAG GCCCAGGGTT GCCCTATCAG
    TGCCAGTTTT GTGACAAATC GTTCAGCCGC CTCAGCTACT TAAAGCACCA CGAGCAAAGT
    CACAGTGACA AACTCCCTTT CAAATGCACT TATTGCAGTC GTCTCTTCAA ACACAAACGA
    AGCAGGGACC GCCACATAAA GCTTCATACG GGAGACAAGA AGTATCACTG CAGTGAGTGT
    GATGCAGCAT TCTCAAGGAG CGATCATCTT AAAATTCACT TAAAGACTCA TACGTCCAAC
    AAGCCATATA AATGTGCCAT TTGTAGACGT GGATTCCTGT CATCCAGTTC GCTGCACGGC
    CACATGCAGG TTCACGAGAG GAACAAGGAT GGCTCTCAGT CTGCTTCCCG GATGGAGGAC
    TGGAAAATGA AGGACACTCA GAAGTGCAGT CAGTGTGAAG AAGGCTTTGA TTTTCCTGAA
    GATCTTCAGA AGCACATTGC GGAATGTCAC CCTGAGTGTT CTCCTAATGA AGACCGATCT
    GCTCTCCAGT GTGTTTACTG TCACGAACTC TTTGTAGAGG AAACGTCTCT GGTGAATCAC
    ATGGAGCAGG CTCATAATGG TGAGAAGAAG AATTCATGCA GCATTTGCTC TGAGAACTTT
    CACACTGTGG AAGAGCTGTA CAGCCACATG GACAGTCACC AGCAGCCAGA ATCATGCAAC
    CACAGCAACA GCCCATCATT GGTAACTGTT GGCTACACCT CAGTCTCAAG TACCACTCCA
    GATTCCAATC TCTCTGTGGA TAGTTCAACA ATGGTGGAAA CGGCTCCCCC TATACCAAAA
    GGCCGTGGAA GAAAGCGGGC AGCTCAGCAA GTGCCAGATA TCACTGGTCC TTCAAGTAAA
    CAGGCAAAAG TTACCTATAG CTGCATTTAT TGCAACAAAC AGTTATTTTC CAGCCTTGCA
    GTTTTGCAGA TACACCTGAA AACTATGCAT TTAGATAAAC CTGAACAAGC CCATATCTGT
    CAGTATTGTT TGGAGGTATT GCCATCTCTC TACAATCTGA ACGAACATCT TAAGCAAGTC
    CACGAAGCTC CGGATCCAGG GCTGATTGTT TCCACTATGC CTGCCATGGT GTACCAGTGC
    AACTTCTGCT CTGAAGTGTT CAATGACCTC AACACGCTTC AAGAACATAT CCGATGTTCT
    CATGGATTTG CCAACCCTGC TGCTAAGGAC AGTAATGCGT TCTTTTGCCC CCATTGCTAC
    ATGGGGTTTC TTACAGATTC TTCTCTGGAA GAGCATATTA GACAAGTCCA TTGTGATCTT
    AGCAGTTCCC GTTTTGGTTC CCCTGTGCTC GGAACCCCAA AAGATCCAGT AGTGGAAGTG
    TATTCTTGTT CTTACTGTAC AAATTCTCCA ATATTTAATA GCGTTCTTAA ACTGAACAAG
    CATATCAAGG AGAACCATAA GAACATTCCT TTGGCACTGA ATTACATCCA CAATGGAAAA
    AAATCCAGAG CCATGAGTCC CTTATCTCCT GTAACGATTG AGCAGACCTC TTTAAAAATG
    ATGCAGGCGG TTGGAGGTGC TCCTCCTCGT CCTGCAGGTG AATATATTTG TAATCAATGT
    GGTGCTAAGT ATACTTCTTT GGACGGTTTT CAGACTCATT TAAAAACACA TCTTGATACT
    GTCCTGCCAA AACTGACCTG CCCTCAGTGC AACAAGGAGT TTCCAAATCA GGAGTCTCTG
    CTGAAGCATG TTACCATTCA TTTTATGATC ACTTCAACCT ACTACATCTG TGAAAGCTGT
    GACAAGCAGT TTACTTCTGT GGATGACTTG CAGAAGCACC TACTAGACAT GCATACATTT
    GTATTCTTCC GCTGCACCTT GTGCCAAGAG GTTTTTGACT CCAAAGTCTC CATCCAACTA
    CACTTGGCTG TGAAGCACAG CAATGAAAAG AAGGTATATA GGTGTACGTC TTGCAACTGG
    GATTTCCGCA ATGAGACTGA CCTACAGCTT CACGTTAAAC ACAACCACTT GGAGAACCAA
    GGCAAAGTGC ACAAGTGCAT CTTCTGTGGT GAGTCCTTTG GTACAGAGGT GGAGCTGCAG
    TGCCATATTA CTACGCACAG CAAGAAGTAC AACTGTAAGT TCTGCAGCAA AGCTTTCCAT
    GCCATCATCC TGCTGGAAAA GCATTTAAGG GAAAAACATT GTGTTTTTGA AACAAAAACT
    CCAAATTGTG GAACGAATGG GGCATCTGAA CAGGTTCAGA AGGAAGAAGT GGAGCTCCAG
    ACCTTGTTGA CAAATAGCCA GGAGTCCCAT AACAGCCATG ATGGCAGCGA AGAGGATGTG
    GACACATCTG AGCCTATGTA TGGCTGTGAC ATTTGTGGAG CGGCTTACAC CATGGAGACT
    CTCCTGCAAA ACCACCAGCT TCGAGACCAC AACATTCGAC CTGGAGAAAG TGCCATAGTG
    AAGAAAAAGG CTGAACTCAT TAAAGGGAAT TACAAGTGTA ATGTGTGTTC TCGAACATTC
    TTCTCTGAAA ATGGGCTCCG AGAACACATG CAGACACACT TGGGACCAGT GAAACATTAC
    ATGTGCCCAA TCTGTGGCGA GCGGTTTCCA TCTCTTCTGA CTCTTACTGA ACATAAAGTC
    ACACATAGTA AGAGCCTGGA CACCGGAAAC TGCAGGATTT GCAAGATGCC TCTCCAGAGT
    GAGGAGGAAT TTTTAGAGCA TTGCCAGATG CACCCTGACT TGAGGAACTC TTTGACAGGG
    TTCCGCTGTG TGGTGTGCAT GCAGACTGTG ACCTCCACGT TGGAACTGAA AATCCACGGC
    ACATTCCACA TGCAGAAAAC TGGAAATGGT TCTGCCGTGC AGTCCACGGG GCGCGCACAG
    CATCTCCAGA AACTGTACAA GTGTGCTTCT TGCCTGAAGG AATTTCGTTC AAAACAGGAT
    TTAGTGAAAC TTGACATCAA TGGTCTGCCA TATGGTCTGT GTGCTAGCTG TGTTAATCTC
    AGTAAAAGTG GTAGTCCAAG TGTCAACATC CCATCAAGCA GTAACAGACA AGGCATGGGC
    CAAAATGAGA ACTTGAGTTC CATTGAGAAC AAAAGCAAGG CAGGGGGACT GAAGACTCGA
    TGTTCTAGTT GCAATGTTAA ATTTGAATCA GAAAGTGAAC TCCAAAACCA TATTCAGTCA
    ATCCACAGAG AGCTTGTTCC AGACAGCAAC AGTACACAGC TGAAAACACC ACAAGTATCT
    CCAATGCCCA GAATCAGCCC CTCCCAAACT GAAGAGAAGA AGACCTACCA GTGCATCAAA
    TGTCAGATGG TTTTCTACAA TGAATGGGAT ATCCAAGTTC ATGTTGCAAA CCACATGATT
    GATGAAGGAC TGAACCATGA ATGCAAGCTC TGCAATCAGA CATTTGACTC TCCTGCCAAA
    CTTCAGTGCC ACCTGATAGA GCACAGCTTT GAAGGCATGG GGGGCACCTT CAAATGTCCA
    GTGTGCTTTA CAGTGTTTGT CCAAGCAAAC AAGTTGCAGC AGCACATTTT CTCAGCCCAT
    GGACAAGAGG ACAAGATCTA TGACTGCACA CAGTGTCCAC AGAAGTTCTT CTTCCAAACA
    GAGCTGCAGA ATCATACGAT GACCCAGCAT AGCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133474.1
    CDS174..4232
    Translation

    Target ORF information:

    RefSeq Version XM_015277988.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277988.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCTCGCC GCAAGCAAGC TAAACCGAGA TCACTCAAAG AAACCCAGAT TCTAGAAACA 
    ACCAATTGTA TGAAGCCTTG GGGTTGGCGG AGGAGGAAAA GAGTAGAAGA AAATGAAACA
    GAGGACCTGC AGGCAGGTGT GGGACACACA GCCGCGCAGA CTGACCCTAA CTGTAAACTA
    GAAGACAAGA CAGAAGATGG AGAAGTGTTA GACTGTAAGA AAAGGCCGGA TGAAGGGGAG
    GAGTTGGAAG AAGAAGCCGT GCACAGCTGT GACAGCTGCC TCCAGGTGTT TGAGTCGCTG
    AGTGATATCA CAGAACACAA GATTAATCAA TGTCAACTGA CAGATGGAGT GGATGTTGAG
    GATGACCCCA CTTGCTCATG GCCAGCATCA TCGCCTTCCA GCAAGGACCA GACTTCCCCA
    AGCCATGGAG AAGGTTGTGA TTTTGGTGAG GAGGAAGGAG GCCCAGGGTT GCCCTATCAG
    TGCCAGTTTT GTGACAAATC GTTCAGCCGC CTCAGCTACT TAAAGCACCA CGAGCAAAGT
    CACAGTGACA AACTCCCTTT CAAATGCACT TATTGCAGTC GTCTCTTCAA ACACAAACGA
    AGCAGGGACC GCCACATAAA GCTTCATACG GGAGACAAGA AGTATCACTG CAGTGAGTGT
    GATGCAGCAT TCTCAAGGAG CGATCATCTT AAAATTCACT TAAAGACTCA TACGTCCAAC
    AAGCCATATA AATGTGCCAT TTGTAGACGT GGATTCCTGT CATCCAGTTC GCTGCACGGC
    CACATGCAGG TTCACGAGAG GAACAAGGAT GGCTCTCAGT CTGCTTCCCG GATGGAGGAC
    TGGAAAATGA AGGACACTCA GAAGTGCAGT CAGTGTGAAG AAGGCTTTGA TTTTCCTGAA
    GATCTTCAGA AGCACATTGC GGAATGTCAC CCTGAGTGTT CTCCTAATGA AGACCGATCT
    GCTCTCCAGT GTGTTTACTG TCACGAACTC TTTGTAGAGG AAACGTCTCT GGTGAATCAC
    ATGGAGCAGG CTCATAATGG TGAGAAGAAG AATTCATGCA GCATTTGCTC TGAGAACTTT
    CACACTGTGG AAGAGCTGTA CAGCCACATG GACAGTCACC AGCAGCCAGA ATCATGCAAC
    CACAGCAACA GCCCATCATT GGTAACTGTT GGCTACACCT CAGTCTCAAG TACCACTCCA
    GATTCCAATC TCTCTGTGGA TAGTTCAACA ATGGTGGAAA CGGCTCCCCC TATACCAAAA
    GGCCGTGGAA GAAAGCGGGC AGCTCAGCAA GTGCCAGATA TCACTGGTCC TTCAAGTAAA
    CAGGCAAAAG TTACCTATAG CTGCATTTAT TGCAACAAAC AGTTATTTTC CAGCCTTGCA
    GTTTTGCAGA TACACCTGAA AACTATGCAT TTAGATAAAC CTGAACAAGC CCATATCTGT
    CAGTATTGTT TGGAGGTATT GCCATCTCTC TACAATCTGA ACGAACATCT TAAGCAAGTC
    CACGAAGCTC CGGATCCAGG GCTGATTGTT TCCACTATGC CTGCCATGGT GTACCAGTGC
    AACTTCTGCT CTGAAGTGTT CAATGACCTC AACACGCTTC AAGAACATAT CCGATGTTCT
    CATGGATTTG CCAACCCTGC TGCTAAGGAC AGTAATGCGT TCTTTTGCCC CCATTGCTAC
    ATGGGGTTTC TTACAGATTC TTCTCTGGAA GAGCATATTA GACAAGTCCA TTGTGATCTT
    AGCAGTTCCC GTTTTGGTTC CCCTGTGCTC GGAACCCCAA AAGATCCAGT AGTGGAAGTG
    TATTCTTGTT CTTACTGTAC AAATTCTCCA ATATTTAATA GCGTTCTTAA ACTGAACAAG
    CATATCAAGG AGAACCATAA GAACATTCCT TTGGCACTGA ATTACATCCA CAATGGAAAA
    AAATCCAGAG CCATGAGTCC CTTATCTCCT GTAACGATTG AGCAGACCTC TTTAAAAATG
    ATGCAGGCGG TTGGAGGTGC TCCTCCTCGT CCTGCAGGTG AATATATTTG TAATCAATGT
    GGTGCTAAGT ATACTTCTTT GGACGGTTTT CAGACTCATT TAAAAACACA TCTTGATACT
    GTCCTGCCAA AACTGACCTG CCCTCAGTGC AACAAGGAGT TTCCAAATCA GGAGTCTCTG
    CTGAAGCATG TTACCATTCA TTTTATGATC ACTTCAACCT ACTACATCTG TGAAAGCTGT
    GACAAGCAGT TTACTTCTGT GGATGACTTG CAGAAGCACC TACTAGACAT GCATACATTT
    GTATTCTTCC GCTGCACCTT GTGCCAAGAG GTTTTTGACT CCAAAGTCTC CATCCAACTA
    CACTTGGCTG TGAAGCACAG CAATGAAAAG AAGGTATATA GGTGTACGTC TTGCAACTGG
    GATTTCCGCA ATGAGACTGA CCTACAGCTT CACGTTAAAC ACAACCACTT GGAGAACCAA
    GGCAAAGTGC ACAAGTGCAT CTTCTGTGGT GAGTCCTTTG GTACAGAGGT GGAGCTGCAG
    TGCCATATTA CTACGCACAG CAAGAAGTAC AACTGTAAGT TCTGCAGCAA AGCTTTCCAT
    GCCATCATCC TGCTGGAAAA GCATTTAAGG GAAAAACATT GTGTTTTTGA AACAAAAACT
    CCAAATTGTG GAACGAATGG GGCATCTGAA CAGGTTCAGA AGGAAGAAGT GGAGCTCCAG
    ACCTTGTTGA CAAATAGCCA GGAGTCCCAT AACAGCCATG ATGGCAGCGA AGAGGATGTG
    GACACATCTG AGCCTATGTA TGGCTGTGAC ATTTGTGGAG CGGCTTACAC CATGGAGACT
    CTCCTGCAAA ACCACCAGCT TCGAGACCAC AACATTCGAC CTGGAGAAAG TGCCATAGTG
    AAGAAAAAGG CTGAACTCAT TAAAGGGAAT TACAAGTGTA ATGTGTGTTC TCGAACATTC
    TTCTCTGAAA ATGGGCTCCG AGAACACATG CAGACACACT TGGGACCAGT GAAACATTAC
    ATGTGCCCAA TCTGTGGCGA GCGGTTTCCA TCTCTTCTGA CTCTTACTGA ACATAAAGTC
    ACACATAGTA AGAGCCTGGA CACCGGAAAC TGCAGGATTT GCAAGATGCC TCTCCAGAGT
    GAGGAGGAAT TTTTAGAGCA TTGCCAGATG CACCCTGACT TGAGGAACTC TTTGACAGGG
    TTCCGCTGTG TGGTGTGCAT GCAGACTGTG ACCTCCACGT TGGAACTGAA AATCCACGGC
    ACATTCCACA TGCAGAAAAC TGGAAATGGT TCTGCCGTGC AGTCCACGGG GCGCGCACAG
    CATCTCCAGA AACTGTACAA GTGTGCTTCT TGCCTGAAGG AATTTCGTTC AAAACAGGAT
    TTAGTGAAAC TTGACATCAA TGGTCTGCCA TATGGTCTGT GTGCTAGCTG TGTTAATCTC
    AGTAAAAGTG GTAGTCCAAG TGTCAACATC CCATCAAGCA GTAACAGACA AGGCATGGGC
    CAAAATGAGA ACTTGAGTTC CATTGAGAAC AAAAGCAAGG CAGGGGGACT GAAGACTCGA
    TGTTCTAGTT GCAATGTTAA ATTTGAATCA GAAAGTGAAC TCCAAAACCA TATTCAGTCA
    ATCCACAGAG AGCTTGTTCC AGACAGCAAC AGTACACAGC TGAAAACACC ACAAGTATCT
    CCAATGCCCA GAATCAGCCC CTCCCAAACT GAAGAGAAGA AGACCTACCA GTGCATCAAA
    TGTCAGATGG TTTTCTACAA TGAATGGGAT ATCCAAGTTC ATGTTGCAAA CCACATGATT
    GATGAAGGAC TGAACCATGA ATGCAAGCTC TGCAATCAGA CATTTGACTC TCCTGCCAAA
    CTTCAGTGCC ACCTGATAGA GCACAGCTTT GAAGGCATGG GGGGCACCTT CAAATGTCCA
    GTGTGCTTTA CAGTGTTTGT CCAAGCAAAC AAGTTGCAGC AGCACATTTT CTCAGCCCAT
    GGACAAGAGG ACAAGATCTA TGACTGCACA CAGTGTCCAC AGAAGTTCTT CTTCCAAACA
    GAGCTGCAGA ATCATACGAT GACCCAGCAT AGCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32512
    Clone ID Related Accession (Same CDS sequence) XM_015277988.1 , XM_015277988.2
    Accession Version XM_015277988.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product zinc finger protein 521 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277988.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCTCGCC GCAAGCAAGC TAAACCGAGA TCACTCAAAG AAACCCAGAT TCTAGAAACA 
    ACCAATTGTA TGAAGCCTTG GGGTTGGCGG AGGAGGAAAA GAGTAGAAGA AAATGAAACA
    GAGGACCTGC AGGCAGGTGT GGGACACACA GCCGCGCAGA CTGACCCTAA CTGTAAACTA
    GAAGACAAGA CAGAAGATGG AGAAGTGTTA GACTGTAAGA AAAGGCCGGA TGAAGGGGAG
    GAGTTGGAAG AAGAAGCCGT GCACAGCTGT GACAGCTGCC TCCAGGTGTT TGAGTCGCTG
    AGTGATATCA CAGAACACAA GATTAATCAA TGTCAACTGA CAGATGGAGT GGATGTTGAG
    GATGACCCCA CTTGCTCATG GCCAGCATCA TCGCCTTCCA GCAAGGACCA GACTTCCCCA
    AGCCATGGAG AAGGTTGTGA TTTTGGTGAG GAGGAAGGAG GCCCAGGGTT GCCCTATCAG
    TGCCAGTTTT GTGACAAATC GTTCAGCCGC CTCAGCTACT TAAAGCACCA CGAGCAAAGT
    CACAGTGACA AACTCCCTTT CAAATGCACT TATTGCAGTC GTCTCTTCAA ACACAAACGA
    AGCAGGGACC GCCACATAAA GCTTCATACG GGAGACAAGA AGTATCACTG CAGTGAGTGT
    GATGCAGCAT TCTCAAGGAG CGATCATCTT AAAATTCACT TAAAGACTCA TACGTCCAAC
    AAGCCATATA AATGTGCCAT TTGTAGACGT GGATTCCTGT CATCCAGTTC GCTGCACGGC
    CACATGCAGG TTCACGAGAG GAACAAGGAT GGCTCTCAGT CTGCTTCCCG GATGGAGGAC
    TGGAAAATGA AGGACACTCA GAAGTGCAGT CAGTGTGAAG AAGGCTTTGA TTTTCCTGAA
    GATCTTCAGA AGCACATTGC GGAATGTCAC CCTGAGTGTT CTCCTAATGA AGACCGATCT
    GCTCTCCAGT GTGTTTACTG TCACGAACTC TTTGTAGAGG AAACGTCTCT GGTGAATCAC
    ATGGAGCAGG CTCATAATGG TGAGAAGAAG AATTCATGCA GCATTTGCTC TGAGAACTTT
    CACACTGTGG AAGAGCTGTA CAGCCACATG GACAGTCACC AGCAGCCAGA ATCATGCAAC
    CACAGCAACA GCCCATCATT GGTAACTGTT GGCTACACCT CAGTCTCAAG TACCACTCCA
    GATTCCAATC TCTCTGTGGA TAGTTCAACA ATGGTGGAAA CGGCTCCCCC TATACCAAAA
    GGCCGTGGAA GAAAGCGGGC AGCTCAGCAA GTGCCAGATA TCACTGGTCC TTCAAGTAAA
    CAGGCAAAAG TTACCTATAG CTGCATTTAT TGCAACAAAC AGTTATTTTC CAGCCTTGCA
    GTTTTGCAGA TACACCTGAA AACTATGCAT TTAGATAAAC CTGAACAAGC CCATATCTGT
    CAGTATTGTT TGGAGGTATT GCCATCTCTC TACAATCTGA ACGAACATCT TAAGCAAGTC
    CACGAAGCTC CGGATCCAGG GCTGATTGTT TCCACTATGC CTGCCATGGT GTACCAGTGC
    AACTTCTGCT CTGAAGTGTT CAATGACCTC AACACGCTTC AAGAACATAT CCGATGTTCT
    CATGGATTTG CCAACCCTGC TGCTAAGGAC AGTAATGCGT TCTTTTGCCC CCATTGCTAC
    ATGGGGTTTC TTACAGATTC TTCTCTGGAA GAGCATATTA GACAAGTCCA TTGTGATCTT
    AGCAGTTCCC GTTTTGGTTC CCCTGTGCTC GGAACCCCAA AAGATCCAGT AGTGGAAGTG
    TATTCTTGTT CTTACTGTAC AAATTCTCCA ATATTTAATA GCGTTCTTAA ACTGAACAAG
    CATATCAAGG AGAACCATAA GAACATTCCT TTGGCACTGA ATTACATCCA CAATGGAAAA
    AAATCCAGAG CCATGAGTCC CTTATCTCCT GTAACGATTG AGCAGACCTC TTTAAAAATG
    ATGCAGGCGG TTGGAGGTGC TCCTCCTCGT CCTGCAGGTG AATATATTTG TAATCAATGT
    GGTGCTAAGT ATACTTCTTT GGACGGTTTT CAGACTCATT TAAAAACACA TCTTGATACT
    GTCCTGCCAA AACTGACCTG CCCTCAGTGC AACAAGGAGT TTCCAAATCA GGAGTCTCTG
    CTGAAGCATG TTACCATTCA TTTTATGATC ACTTCAACCT ACTACATCTG TGAAAGCTGT
    GACAAGCAGT TTACTTCTGT GGATGACTTG CAGAAGCACC TACTAGACAT GCATACATTT
    GTATTCTTCC GCTGCACCTT GTGCCAAGAG GTTTTTGACT CCAAAGTCTC CATCCAACTA
    CACTTGGCTG TGAAGCACAG CAATGAAAAG AAGGTATATA GGTGTACGTC TTGCAACTGG
    GATTTCCGCA ATGAGACTGA CCTACAGCTT CACGTTAAAC ACAACCACTT GGAGAACCAA
    GGCAAAGTGC ACAAGTGCAT CTTCTGTGGT GAGTCCTTTG GTACAGAGGT GGAGCTGCAG
    TGCCATATTA CTACGCACAG CAAGAAGTAC AACTGTAAGT TCTGCAGCAA AGCTTTCCAT
    GCCATCATCC TGCTGGAAAA GCATTTAAGG GAAAAACATT GTGTTTTTGA AACAAAAACT
    CCAAATTGTG GAACGAATGG GGCATCTGAA CAGGTTCAGA AGGAAGAAGT GGAGCTCCAG
    ACCTTGTTGA CAAATAGCCA GGAGTCCCAT AACAGCCATG ATGGCAGCGA AGAGGATGTG
    GACACATCTG AGCCTATGTA TGGCTGTGAC ATTTGTGGAG CGGCTTACAC CATGGAGACT
    CTCCTGCAAA ACCACCAGCT TCGAGACCAC AACATTCGAC CTGGAGAAAG TGCCATAGTG
    AAGAAAAAGG CTGAACTCAT TAAAGGGAAT TACAAGTGTA ATGTGTGTTC TCGAACATTC
    TTCTCTGAAA ATGGGCTCCG AGAACACATG CAGACACACT TGGGACCAGT GAAACATTAC
    ATGTGCCCAA TCTGTGGCGA GCGGTTTCCA TCTCTTCTGA CTCTTACTGA ACATAAAGTC
    ACACATAGTA AGAGCCTGGA CACCGGAAAC TGCAGGATTT GCAAGATGCC TCTCCAGAGT
    GAGGAGGAAT TTTTAGAGCA TTGCCAGATG CACCCTGACT TGAGGAACTC TTTGACAGGG
    TTCCGCTGTG TGGTGTGCAT GCAGACTGTG ACCTCCACGT TGGAACTGAA AATCCACGGC
    ACATTCCACA TGCAGAAAAC TGGAAATGGT TCTGCCGTGC AGTCCACGGG GCGCGCACAG
    CATCTCCAGA AACTGTACAA GTGTGCTTCT TGCCTGAAGG AATTTCGTTC AAAACAGGAT
    TTAGTGAAAC TTGACATCAA TGGTCTGCCA TATGGTCTGT GTGCTAGCTG TGTTAATCTC
    AGTAAAAGTG GTAGTCCAAG TGTCAACATC CCATCAAGCA GTAACAGACA AGGCATGGGC
    CAAAATGAGA ACTTGAGTTC CATTGAGAAC AAAAGCAAGG CAGGGGGACT GAAGACTCGA
    TGTTCTAGTT GCAATGTTAA ATTTGAATCA GAAAGTGAAC TCCAAAACCA TATTCAGTCA
    ATCCACAGAG AGCTTGTTCC AGACAGCAAC AGTACACAGC TGAAAACACC ACAAGTATCT
    CCAATGCCCA GAATCAGCCC CTCCCAAACT GAAGAGAAGA AGACCTACCA GTGCATCAAA
    TGTCAGATGG TTTTCTACAA TGAATGGGAT ATCCAAGTTC ATGTTGCAAA CCACATGATT
    GATGAAGGAC TGAACCATGA ATGCAAGCTC TGCAATCAGA CATTTGACTC TCCTGCCAAA
    CTTCAGTGCC ACCTGATAGA GCACAGCTTT GAAGGCATGG GGGGCACCTT CAAATGTCCA
    GTGTGCTTTA CAGTGTTTGT CCAAGCAAAC AAGTTGCAGC AGCACATTTT CTCAGCCCAT
    GGACAAGAGG ACAAGATCTA TGACTGCACA CAGTGTCCAC AGAAGTTCTT CTTCCAAACA
    GAGCTGCAGA ATCATACGAT GACCCAGCAT AGCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133474.1
    CDS174..4232
    Translation

    Target ORF information:

    RefSeq Version XM_015277988.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277988.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCTCGCC GCAAGCAAGC TAAACCGAGA TCACTCAAAG AAACCCAGAT TCTAGAAACA 
    ACCAATTGTA TGAAGCCTTG GGGTTGGCGG AGGAGGAAAA GAGTAGAAGA AAATGAAACA
    GAGGACCTGC AGGCAGGTGT GGGACACACA GCCGCGCAGA CTGACCCTAA CTGTAAACTA
    GAAGACAAGA CAGAAGATGG AGAAGTGTTA GACTGTAAGA AAAGGCCGGA TGAAGGGGAG
    GAGTTGGAAG AAGAAGCCGT GCACAGCTGT GACAGCTGCC TCCAGGTGTT TGAGTCGCTG
    AGTGATATCA CAGAACACAA GATTAATCAA TGTCAACTGA CAGATGGAGT GGATGTTGAG
    GATGACCCCA CTTGCTCATG GCCAGCATCA TCGCCTTCCA GCAAGGACCA GACTTCCCCA
    AGCCATGGAG AAGGTTGTGA TTTTGGTGAG GAGGAAGGAG GCCCAGGGTT GCCCTATCAG
    TGCCAGTTTT GTGACAAATC GTTCAGCCGC CTCAGCTACT TAAAGCACCA CGAGCAAAGT
    CACAGTGACA AACTCCCTTT CAAATGCACT TATTGCAGTC GTCTCTTCAA ACACAAACGA
    AGCAGGGACC GCCACATAAA GCTTCATACG GGAGACAAGA AGTATCACTG CAGTGAGTGT
    GATGCAGCAT TCTCAAGGAG CGATCATCTT AAAATTCACT TAAAGACTCA TACGTCCAAC
    AAGCCATATA AATGTGCCAT TTGTAGACGT GGATTCCTGT CATCCAGTTC GCTGCACGGC
    CACATGCAGG TTCACGAGAG GAACAAGGAT GGCTCTCAGT CTGCTTCCCG GATGGAGGAC
    TGGAAAATGA AGGACACTCA GAAGTGCAGT CAGTGTGAAG AAGGCTTTGA TTTTCCTGAA
    GATCTTCAGA AGCACATTGC GGAATGTCAC CCTGAGTGTT CTCCTAATGA AGACCGATCT
    GCTCTCCAGT GTGTTTACTG TCACGAACTC TTTGTAGAGG AAACGTCTCT GGTGAATCAC
    ATGGAGCAGG CTCATAATGG TGAGAAGAAG AATTCATGCA GCATTTGCTC TGAGAACTTT
    CACACTGTGG AAGAGCTGTA CAGCCACATG GACAGTCACC AGCAGCCAGA ATCATGCAAC
    CACAGCAACA GCCCATCATT GGTAACTGTT GGCTACACCT CAGTCTCAAG TACCACTCCA
    GATTCCAATC TCTCTGTGGA TAGTTCAACA ATGGTGGAAA CGGCTCCCCC TATACCAAAA
    GGCCGTGGAA GAAAGCGGGC AGCTCAGCAA GTGCCAGATA TCACTGGTCC TTCAAGTAAA
    CAGGCAAAAG TTACCTATAG CTGCATTTAT TGCAACAAAC AGTTATTTTC CAGCCTTGCA
    GTTTTGCAGA TACACCTGAA AACTATGCAT TTAGATAAAC CTGAACAAGC CCATATCTGT
    CAGTATTGTT TGGAGGTATT GCCATCTCTC TACAATCTGA ACGAACATCT TAAGCAAGTC
    CACGAAGCTC CGGATCCAGG GCTGATTGTT TCCACTATGC CTGCCATGGT GTACCAGTGC
    AACTTCTGCT CTGAAGTGTT CAATGACCTC AACACGCTTC AAGAACATAT CCGATGTTCT
    CATGGATTTG CCAACCCTGC TGCTAAGGAC AGTAATGCGT TCTTTTGCCC CCATTGCTAC
    ATGGGGTTTC TTACAGATTC TTCTCTGGAA GAGCATATTA GACAAGTCCA TTGTGATCTT
    AGCAGTTCCC GTTTTGGTTC CCCTGTGCTC GGAACCCCAA AAGATCCAGT AGTGGAAGTG
    TATTCTTGTT CTTACTGTAC AAATTCTCCA ATATTTAATA GCGTTCTTAA ACTGAACAAG
    CATATCAAGG AGAACCATAA GAACATTCCT TTGGCACTGA ATTACATCCA CAATGGAAAA
    AAATCCAGAG CCATGAGTCC CTTATCTCCT GTAACGATTG AGCAGACCTC TTTAAAAATG
    ATGCAGGCGG TTGGAGGTGC TCCTCCTCGT CCTGCAGGTG AATATATTTG TAATCAATGT
    GGTGCTAAGT ATACTTCTTT GGACGGTTTT CAGACTCATT TAAAAACACA TCTTGATACT
    GTCCTGCCAA AACTGACCTG CCCTCAGTGC AACAAGGAGT TTCCAAATCA GGAGTCTCTG
    CTGAAGCATG TTACCATTCA TTTTATGATC ACTTCAACCT ACTACATCTG TGAAAGCTGT
    GACAAGCAGT TTACTTCTGT GGATGACTTG CAGAAGCACC TACTAGACAT GCATACATTT
    GTATTCTTCC GCTGCACCTT GTGCCAAGAG GTTTTTGACT CCAAAGTCTC CATCCAACTA
    CACTTGGCTG TGAAGCACAG CAATGAAAAG AAGGTATATA GGTGTACGTC TTGCAACTGG
    GATTTCCGCA ATGAGACTGA CCTACAGCTT CACGTTAAAC ACAACCACTT GGAGAACCAA
    GGCAAAGTGC ACAAGTGCAT CTTCTGTGGT GAGTCCTTTG GTACAGAGGT GGAGCTGCAG
    TGCCATATTA CTACGCACAG CAAGAAGTAC AACTGTAAGT TCTGCAGCAA AGCTTTCCAT
    GCCATCATCC TGCTGGAAAA GCATTTAAGG GAAAAACATT GTGTTTTTGA AACAAAAACT
    CCAAATTGTG GAACGAATGG GGCATCTGAA CAGGTTCAGA AGGAAGAAGT GGAGCTCCAG
    ACCTTGTTGA CAAATAGCCA GGAGTCCCAT AACAGCCATG ATGGCAGCGA AGAGGATGTG
    GACACATCTG AGCCTATGTA TGGCTGTGAC ATTTGTGGAG CGGCTTACAC CATGGAGACT
    CTCCTGCAAA ACCACCAGCT TCGAGACCAC AACATTCGAC CTGGAGAAAG TGCCATAGTG
    AAGAAAAAGG CTGAACTCAT TAAAGGGAAT TACAAGTGTA ATGTGTGTTC TCGAACATTC
    TTCTCTGAAA ATGGGCTCCG AGAACACATG CAGACACACT TGGGACCAGT GAAACATTAC
    ATGTGCCCAA TCTGTGGCGA GCGGTTTCCA TCTCTTCTGA CTCTTACTGA ACATAAAGTC
    ACACATAGTA AGAGCCTGGA CACCGGAAAC TGCAGGATTT GCAAGATGCC TCTCCAGAGT
    GAGGAGGAAT TTTTAGAGCA TTGCCAGATG CACCCTGACT TGAGGAACTC TTTGACAGGG
    TTCCGCTGTG TGGTGTGCAT GCAGACTGTG ACCTCCACGT TGGAACTGAA AATCCACGGC
    ACATTCCACA TGCAGAAAAC TGGAAATGGT TCTGCCGTGC AGTCCACGGG GCGCGCACAG
    CATCTCCAGA AACTGTACAA GTGTGCTTCT TGCCTGAAGG AATTTCGTTC AAAACAGGAT
    TTAGTGAAAC TTGACATCAA TGGTCTGCCA TATGGTCTGT GTGCTAGCTG TGTTAATCTC
    AGTAAAAGTG GTAGTCCAAG TGTCAACATC CCATCAAGCA GTAACAGACA AGGCATGGGC
    CAAAATGAGA ACTTGAGTTC CATTGAGAAC AAAAGCAAGG CAGGGGGACT GAAGACTCGA
    TGTTCTAGTT GCAATGTTAA ATTTGAATCA GAAAGTGAAC TCCAAAACCA TATTCAGTCA
    ATCCACAGAG AGCTTGTTCC AGACAGCAAC AGTACACAGC TGAAAACACC ACAAGTATCT
    CCAATGCCCA GAATCAGCCC CTCCCAAACT GAAGAGAAGA AGACCTACCA GTGCATCAAA
    TGTCAGATGG TTTTCTACAA TGAATGGGAT ATCCAAGTTC ATGTTGCAAA CCACATGATT
    GATGAAGGAC TGAACCATGA ATGCAAGCTC TGCAATCAGA CATTTGACTC TCCTGCCAAA
    CTTCAGTGCC ACCTGATAGA GCACAGCTTT GAAGGCATGG GGGGCACCTT CAAATGTCCA
    GTGTGCTTTA CAGTGTTTGT CCAAGCAAAC AAGTTGCAGC AGCACATTTT CTCAGCCCAT
    GGACAAGAGG ACAAGATCTA TGACTGCACA CAGTGTCCAC AGAAGTTCTT CTTCCAAACA
    GAGCTGCAGA ATCATACGAT GACCCAGCAT AGCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32513
    Clone ID Related Accession (Same CDS sequence) XM_015277989.2 , XM_015277989.1
    Accession Version XM_015277989.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product zinc finger protein 521 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277989.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCTTTGG GGGCCTTGCT TCCATTTTCC ATTATTATGT GGACTACCGG AGCGACAGCG 
    CAGTCCAAGA CCTTGCAGCT GTTTGCTTTT AGGATGTCTC GCCGCAAGCA AGCTAAACCG
    AGATCACTCA AAGACCCTAA CTGTAAACTA GAAGACAAGA CAGAAGATGG AGAAGTGTTA
    GACTGTAAGA AAAGGCCGGA TGAAGGGGAG GAGTTGGAAG AAGAAGCCGT GCACAGCTGT
    GACAGCTGCC TCCAGGTGTT TGAGTCGCTG AGTGATATCA CAGAACACAA GATTAATCAA
    TGTCAACTGA CAGATGGAGT GGATGTTGAG GATGACCCCA CTTGCTCATG GCCAGCATCA
    TCGCCTTCCA GCAAGGACCA GACTTCCCCA AGCCATGGAG AAGGTTGTGA TTTTGGTGAG
    GAGGAAGGAG GCCCAGGGTT GCCCTATCAG TGCCAGTTTT GTGACAAATC GTTCAGCCGC
    CTCAGCTACT TAAAGCACCA CGAGCAAAGT CACAGTGACA AACTCCCTTT CAAATGCACT
    TATTGCAGTC GTCTCTTCAA ACACAAACGA AGCAGGGACC GCCACATAAA GCTTCATACG
    GGAGACAAGA AGTATCACTG CAGTGAGTGT GATGCAGCAT TCTCAAGGAG CGATCATCTT
    AAAATTCACT TAAAGACTCA TACGTCCAAC AAGCCATATA AATGTGCCAT TTGTAGACGT
    GGATTCCTGT CATCCAGTTC GCTGCACGGC CACATGCAGG TTCACGAGAG GAACAAGGAT
    GGCTCTCAGT CTGCTTCCCG GATGGAGGAC TGGAAAATGA AGGACACTCA GAAGTGCAGT
    CAGTGTGAAG AAGGCTTTGA TTTTCCTGAA GATCTTCAGA AGCACATTGC GGAATGTCAC
    CCTGAGTGTT CTCCTAATGA AGACCGATCT GCTCTCCAGT GTGTTTACTG TCACGAACTC
    TTTGTAGAGG AAACGTCTCT GGTGAATCAC ATGGAGCAGG CTCATAATGG TGAGAAGAAG
    AATTCATGCA GCATTTGCTC TGAGAACTTT CACACTGTGG AAGAGCTGTA CAGCCACATG
    GACAGTCACC AGCAGCCAGA ATCATGCAAC CACAGCAACA GCCCATCATT GGTAACTGTT
    GGCTACACCT CAGTCTCAAG TACCACTCCA GATTCCAATC TCTCTGTGGA TAGTTCAACA
    ATGGTGGAAA CGGCTCCCCC TATACCAAAA GGCCGTGGAA GAAAGCGGGC AGCTCAGCAA
    GTGCCAGATA TCACTGGTCC TTCAAGTAAA CAGGCAAAAG TTACCTATAG CTGCATTTAT
    TGCAACAAAC AGTTATTTTC CAGCCTTGCA GTTTTGCAGA TACACCTGAA AACTATGCAT
    TTAGATAAAC CTGAACAAGC CCATATCTGT CAGTATTGTT TGGAGGTATT GCCATCTCTC
    TACAATCTGA ACGAACATCT TAAGCAAGTC CACGAAGCTC CGGATCCAGG GCTGATTGTT
    TCCACTATGC CTGCCATGGT GTACCAGTGC AACTTCTGCT CTGAAGTGTT CAATGACCTC
    AACACGCTTC AAGAACATAT CCGATGTTCT CATGGATTTG CCAACCCTGC TGCTAAGGAC
    AGTAATGCGT TCTTTTGCCC CCATTGCTAC ATGGGGTTTC TTACAGATTC TTCTCTGGAA
    GAGCATATTA GACAAGTCCA TTGTGATCTT AGCAGTTCCC GTTTTGGTTC CCCTGTGCTC
    GGAACCCCAA AAGATCCAGT AGTGGAAGTG TATTCTTGTT CTTACTGTAC AAATTCTCCA
    ATATTTAATA GCGTTCTTAA ACTGAACAAG CATATCAAGG AGAACCATAA GAACATTCCT
    TTGGCACTGA ATTACATCCA CAATGGAAAA AAATCCAGAG CCATGAGTCC CTTATCTCCT
    GTAACGATTG AGCAGACCTC TTTAAAAATG ATGCAGGCGG TTGGAGGTGC TCCTCCTCGT
    CCTGCAGGTG AATATATTTG TAATCAATGT GGTGCTAAGT ATACTTCTTT GGACGGTTTT
    CAGACTCATT TAAAAACACA TCTTGATACT GTCCTGCCAA AACTGACCTG CCCTCAGTGC
    AACAAGGAGT TTCCAAATCA GGAGTCTCTG CTGAAGCATG TTACCATTCA TTTTATGATC
    ACTTCAACCT ACTACATCTG TGAAAGCTGT GACAAGCAGT TTACTTCTGT GGATGACTTG
    CAGAAGCACC TACTAGACAT GCATACATTT GTATTCTTCC GCTGCACCTT GTGCCAAGAG
    GTTTTTGACT CCAAAGTCTC CATCCAACTA CACTTGGCTG TGAAGCACAG CAATGAAAAG
    AAGGTATATA GGTGTACGTC TTGCAACTGG GATTTCCGCA ATGAGACTGA CCTACAGCTT
    CACGTTAAAC ACAACCACTT GGAGAACCAA GGCAAAGTGC ACAAGTGCAT CTTCTGTGGT
    GAGTCCTTTG GTACAGAGGT GGAGCTGCAG TGCCATATTA CTACGCACAG CAAGAAGTAC
    AACTGTAAGT TCTGCAGCAA AGCTTTCCAT GCCATCATCC TGCTGGAAAA GCATTTAAGG
    GAAAAACATT GTGTTTTTGA AACAAAAACT CCAAATTGTG GAACGAATGG GGCATCTGAA
    CAGGTTCAGA AGGAAGAAGT GGAGCTCCAG ACCTTGTTGA CAAATAGCCA GGAGTCCCAT
    AACAGCCATG ATGGCAGCGA AGAGGATGTG GACACATCTG AGCCTATGTA TGGCTGTGAC
    ATTTGTGGAG CGGCTTACAC CATGGAGACT CTCCTGCAAA ACCACCAGCT TCGAGACCAC
    AACATTCGAC CTGGAGAAAG TGCCATAGTG AAGAAAAAGG CTGAACTCAT TAAAGGGAAT
    TACAAGTGTA ATGTGTGTTC TCGAACATTC TTCTCTGAAA ATGGGCTCCG AGAACACATG
    CAGACACACT TGGGACCAGT GAAACATTAC ATGTGCCCAA TCTGTGGCGA GCGGTTTCCA
    TCTCTTCTGA CTCTTACTGA ACATAAAGTC ACACATAGTA AGAGCCTGGA CACCGGAAAC
    TGCAGGATTT GCAAGATGCC TCTCCAGAGT GAGGAGGAAT TTTTAGAGCA TTGCCAGATG
    CACCCTGACT TGAGGAACTC TTTGACAGGG TTCCGCTGTG TGGTGTGCAT GCAGACTGTG
    ACCTCCACGT TGGAACTGAA AATCCACGGC ACATTCCACA TGCAGAAAAC TGGAAATGGT
    TCTGCCGTGC AGTCCACGGG GCGCGCACAG CATCTCCAGA AACTGTACAA GTGTGCTTCT
    TGCCTGAAGG AATTTCGTTC AAAACAGGAT TTAGTGAAAC TTGACATCAA TGGTCTGCCA
    TATGGTCTGT GTGCTAGCTG TGTTAATCTC AGTAAAAGTG GTAGTCCAAG TGTCAACATC
    CCATCAAGCA GTAACAGACA AGGCATGGGC CAAAATGAGA ACTTGAGTTC CATTGAGAAC
    AAAAGCAAGG CAGGGGGACT GAAGACTCGA TGTTCTAGTT GCAATGTTAA ATTTGAATCA
    GAAAGTGAAC TCCAAAACCA TATTCAGTCA ATCCACAGAG AGCTTGTTCC AGACAGCAAC
    AGTACACAGC TGAAAACACC ACAAGTATCT CCAATGCCCA GAATCAGCCC CTCCCAAACT
    GAAGAGAAGA AGACCTACCA GTGCATCAAA TGTCAGATGG TTTTCTACAA TGAATGGGAT
    ATCCAAGTTC ATGTTGCAAA CCACATGATT GATGAAGGAC TGAACCATGA ATGCAAGCTC
    TGCAATCAGA CATTTGACTC TCCTGCCAAA CTTCAGTGCC ACCTGATAGA GCACAGCTTT
    GAAGGCATGG GGGGCACCTT CAAATGTCCA GTGTGCTTTA CAGTGTTTGT CCAAGCAAAC
    AAGTTGCAGC AGCACATTTT CTCAGCCCAT GGACAAGAGG ACAAGATCTA TGACTGCACA
    CAGTGTCCAC AGAAGTTCTT CTTCCAAACA GAGCTGCAGA ATCATACGAT GACCCAGCAT
    AGCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133475.1
    CDS47..4075
    Translation

    Target ORF information:

    RefSeq Version XM_015277989.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277989.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCTTTGG GGGCCTTGCT TCCATTTTCC ATTATTATGT GGACTACCGG AGCGACAGCG 
    CAGTCCAAGA CCTTGCAGCT GTTTGCTTTT AGGATGTCTC GCCGCAAGCA AGCTAAACCG
    AGATCACTCA AAGACCCTAA CTGTAAACTA GAAGACAAGA CAGAAGATGG AGAAGTGTTA
    GACTGTAAGA AAAGGCCGGA TGAAGGGGAG GAGTTGGAAG AAGAAGCCGT GCACAGCTGT
    GACAGCTGCC TCCAGGTGTT TGAGTCGCTG AGTGATATCA CAGAACACAA GATTAATCAA
    TGTCAACTGA CAGATGGAGT GGATGTTGAG GATGACCCCA CTTGCTCATG GCCAGCATCA
    TCGCCTTCCA GCAAGGACCA GACTTCCCCA AGCCATGGAG AAGGTTGTGA TTTTGGTGAG
    GAGGAAGGAG GCCCAGGGTT GCCCTATCAG TGCCAGTTTT GTGACAAATC GTTCAGCCGC
    CTCAGCTACT TAAAGCACCA CGAGCAAAGT CACAGTGACA AACTCCCTTT CAAATGCACT
    TATTGCAGTC GTCTCTTCAA ACACAAACGA AGCAGGGACC GCCACATAAA GCTTCATACG
    GGAGACAAGA AGTATCACTG CAGTGAGTGT GATGCAGCAT TCTCAAGGAG CGATCATCTT
    AAAATTCACT TAAAGACTCA TACGTCCAAC AAGCCATATA AATGTGCCAT TTGTAGACGT
    GGATTCCTGT CATCCAGTTC GCTGCACGGC CACATGCAGG TTCACGAGAG GAACAAGGAT
    GGCTCTCAGT CTGCTTCCCG GATGGAGGAC TGGAAAATGA AGGACACTCA GAAGTGCAGT
    CAGTGTGAAG AAGGCTTTGA TTTTCCTGAA GATCTTCAGA AGCACATTGC GGAATGTCAC
    CCTGAGTGTT CTCCTAATGA AGACCGATCT GCTCTCCAGT GTGTTTACTG TCACGAACTC
    TTTGTAGAGG AAACGTCTCT GGTGAATCAC ATGGAGCAGG CTCATAATGG TGAGAAGAAG
    AATTCATGCA GCATTTGCTC TGAGAACTTT CACACTGTGG AAGAGCTGTA CAGCCACATG
    GACAGTCACC AGCAGCCAGA ATCATGCAAC CACAGCAACA GCCCATCATT GGTAACTGTT
    GGCTACACCT CAGTCTCAAG TACCACTCCA GATTCCAATC TCTCTGTGGA TAGTTCAACA
    ATGGTGGAAA CGGCTCCCCC TATACCAAAA GGCCGTGGAA GAAAGCGGGC AGCTCAGCAA
    GTGCCAGATA TCACTGGTCC TTCAAGTAAA CAGGCAAAAG TTACCTATAG CTGCATTTAT
    TGCAACAAAC AGTTATTTTC CAGCCTTGCA GTTTTGCAGA TACACCTGAA AACTATGCAT
    TTAGATAAAC CTGAACAAGC CCATATCTGT CAGTATTGTT TGGAGGTATT GCCATCTCTC
    TACAATCTGA ACGAACATCT TAAGCAAGTC CACGAAGCTC CGGATCCAGG GCTGATTGTT
    TCCACTATGC CTGCCATGGT GTACCAGTGC AACTTCTGCT CTGAAGTGTT CAATGACCTC
    AACACGCTTC AAGAACATAT CCGATGTTCT CATGGATTTG CCAACCCTGC TGCTAAGGAC
    AGTAATGCGT TCTTTTGCCC CCATTGCTAC ATGGGGTTTC TTACAGATTC TTCTCTGGAA
    GAGCATATTA GACAAGTCCA TTGTGATCTT AGCAGTTCCC GTTTTGGTTC CCCTGTGCTC
    GGAACCCCAA AAGATCCAGT AGTGGAAGTG TATTCTTGTT CTTACTGTAC AAATTCTCCA
    ATATTTAATA GCGTTCTTAA ACTGAACAAG CATATCAAGG AGAACCATAA GAACATTCCT
    TTGGCACTGA ATTACATCCA CAATGGAAAA AAATCCAGAG CCATGAGTCC CTTATCTCCT
    GTAACGATTG AGCAGACCTC TTTAAAAATG ATGCAGGCGG TTGGAGGTGC TCCTCCTCGT
    CCTGCAGGTG AATATATTTG TAATCAATGT GGTGCTAAGT ATACTTCTTT GGACGGTTTT
    CAGACTCATT TAAAAACACA TCTTGATACT GTCCTGCCAA AACTGACCTG CCCTCAGTGC
    AACAAGGAGT TTCCAAATCA GGAGTCTCTG CTGAAGCATG TTACCATTCA TTTTATGATC
    ACTTCAACCT ACTACATCTG TGAAAGCTGT GACAAGCAGT TTACTTCTGT GGATGACTTG
    CAGAAGCACC TACTAGACAT GCATACATTT GTATTCTTCC GCTGCACCTT GTGCCAAGAG
    GTTTTTGACT CCAAAGTCTC CATCCAACTA CACTTGGCTG TGAAGCACAG CAATGAAAAG
    AAGGTATATA GGTGTACGTC TTGCAACTGG GATTTCCGCA ATGAGACTGA CCTACAGCTT
    CACGTTAAAC ACAACCACTT GGAGAACCAA GGCAAAGTGC ACAAGTGCAT CTTCTGTGGT
    GAGTCCTTTG GTACAGAGGT GGAGCTGCAG TGCCATATTA CTACGCACAG CAAGAAGTAC
    AACTGTAAGT TCTGCAGCAA AGCTTTCCAT GCCATCATCC TGCTGGAAAA GCATTTAAGG
    GAAAAACATT GTGTTTTTGA AACAAAAACT CCAAATTGTG GAACGAATGG GGCATCTGAA
    CAGGTTCAGA AGGAAGAAGT GGAGCTCCAG ACCTTGTTGA CAAATAGCCA GGAGTCCCAT
    AACAGCCATG ATGGCAGCGA AGAGGATGTG GACACATCTG AGCCTATGTA TGGCTGTGAC
    ATTTGTGGAG CGGCTTACAC CATGGAGACT CTCCTGCAAA ACCACCAGCT TCGAGACCAC
    AACATTCGAC CTGGAGAAAG TGCCATAGTG AAGAAAAAGG CTGAACTCAT TAAAGGGAAT
    TACAAGTGTA ATGTGTGTTC TCGAACATTC TTCTCTGAAA ATGGGCTCCG AGAACACATG
    CAGACACACT TGGGACCAGT GAAACATTAC ATGTGCCCAA TCTGTGGCGA GCGGTTTCCA
    TCTCTTCTGA CTCTTACTGA ACATAAAGTC ACACATAGTA AGAGCCTGGA CACCGGAAAC
    TGCAGGATTT GCAAGATGCC TCTCCAGAGT GAGGAGGAAT TTTTAGAGCA TTGCCAGATG
    CACCCTGACT TGAGGAACTC TTTGACAGGG TTCCGCTGTG TGGTGTGCAT GCAGACTGTG
    ACCTCCACGT TGGAACTGAA AATCCACGGC ACATTCCACA TGCAGAAAAC TGGAAATGGT
    TCTGCCGTGC AGTCCACGGG GCGCGCACAG CATCTCCAGA AACTGTACAA GTGTGCTTCT
    TGCCTGAAGG AATTTCGTTC AAAACAGGAT TTAGTGAAAC TTGACATCAA TGGTCTGCCA
    TATGGTCTGT GTGCTAGCTG TGTTAATCTC AGTAAAAGTG GTAGTCCAAG TGTCAACATC
    CCATCAAGCA GTAACAGACA AGGCATGGGC CAAAATGAGA ACTTGAGTTC CATTGAGAAC
    AAAAGCAAGG CAGGGGGACT GAAGACTCGA TGTTCTAGTT GCAATGTTAA ATTTGAATCA
    GAAAGTGAAC TCCAAAACCA TATTCAGTCA ATCCACAGAG AGCTTGTTCC AGACAGCAAC
    AGTACACAGC TGAAAACACC ACAAGTATCT CCAATGCCCA GAATCAGCCC CTCCCAAACT
    GAAGAGAAGA AGACCTACCA GTGCATCAAA TGTCAGATGG TTTTCTACAA TGAATGGGAT
    ATCCAAGTTC ATGTTGCAAA CCACATGATT GATGAAGGAC TGAACCATGA ATGCAAGCTC
    TGCAATCAGA CATTTGACTC TCCTGCCAAA CTTCAGTGCC ACCTGATAGA GCACAGCTTT
    GAAGGCATGG GGGGCACCTT CAAATGTCCA GTGTGCTTTA CAGTGTTTGT CCAAGCAAAC
    AAGTTGCAGC AGCACATTTT CTCAGCCCAT GGACAAGAGG ACAAGATCTA TGACTGCACA
    CAGTGTCCAC AGAAGTTCTT CTTCCAAACA GAGCTGCAGA ATCATACGAT GACCCAGCAT
    AGCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32513
    Clone ID Related Accession (Same CDS sequence) XM_015277989.2 , XM_015277989.1
    Accession Version XM_015277989.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product zinc finger protein 521 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCTTTGG GGGCCTTGCT TCCATTTTCC ATTATTATGT GGACTACCGG AGCGACAGCG 
    CAGTCCAAGA CCTTGCAGCT GTTTGCTTTT AGGATGTCTC GCCGCAAGCA AGCTAAACCG
    AGATCACTCA AAGACCCTAA CTGTAAACTA GAAGACAAGA CAGAAGATGG AGAAGTGTTA
    GACTGTAAGA AAAGGCCGGA TGAAGGGGAG GAGTTGGAAG AAGAAGCCGT GCACAGCTGT
    GACAGCTGCC TCCAGGTGTT TGAGTCGCTG AGTGATATCA CAGAACACAA GATTAATCAA
    TGTCAACTGA CAGATGGAGT GGATGTTGAG GATGACCCCA CTTGCTCATG GCCAGCATCA
    TCGCCTTCCA GCAAGGACCA GACTTCCCCA AGCCATGGAG AAGGTTGTGA TTTTGGTGAG
    GAGGAAGGAG GCCCAGGGTT GCCCTATCAG TGCCAGTTTT GTGACAAATC GTTCAGCCGC
    CTCAGCTACT TAAAGCACCA CGAGCAAAGT CACAGTGACA AACTCCCTTT CAAATGCACT
    TATTGCAGTC GTCTCTTCAA ACACAAACGA AGCAGGGACC GCCACATAAA GCTTCATACG
    GGAGACAAGA AGTATCACTG CAGTGAGTGT GATGCAGCAT TCTCAAGGAG CGATCATCTT
    AAAATTCACT TAAAGACTCA TACGTCCAAC AAGCCATATA AATGTGCCAT TTGTAGACGT
    GGATTCCTGT CATCCAGTTC GCTGCACGGC CACATGCAGG TTCACGAGAG GAACAAGGAT
    GGCTCTCAGT CTGCTTCCCG GATGGAGGAC TGGAAAATGA AGGACACTCA GAAGTGCAGT
    CAGTGTGAAG AAGGCTTTGA TTTTCCTGAA GATCTTCAGA AGCACATTGC GGAATGTCAC
    CCTGAGTGTT CTCCTAATGA AGACCGATCT GCTCTCCAGT GTGTTTACTG TCACGAACTC
    TTTGTAGAGG AAACGTCTCT GGTGAATCAC ATGGAGCAGG CTCATAATGG TGAGAAGAAG
    AATTCATGCA GCATTTGCTC TGAGAACTTT CACACTGTGG AAGAGCTGTA CAGCCACATG
    GACAGTCACC AGCAGCCAGA ATCATGCAAC CACAGCAACA GCCCATCATT GGTAACTGTT
    GGCTACACCT CAGTCTCAAG TACCACTCCA GATTCCAATC TCTCTGTGGA TAGTTCAACA
    ATGGTGGAAA CGGCTCCCCC TATACCAAAA GGCCGTGGAA GAAAGCGGGC AGCTCAGCAA
    GTGCCAGATA TCACTGGTCC TTCAAGTAAA CAGGCAAAAG TTACCTATAG CTGCATTTAT
    TGCAACAAAC AGTTATTTTC CAGCCTTGCA GTTTTGCAGA TACACCTGAA AACTATGCAT
    TTAGATAAAC CTGAACAAGC CCATATCTGT CAGTATTGTT TGGAGGTATT GCCATCTCTC
    TACAATCTGA ACGAACATCT TAAGCAAGTC CACGAAGCTC CGGATCCAGG GCTGATTGTT
    TCCACTATGC CTGCCATGGT GTACCAGTGC AACTTCTGCT CTGAAGTGTT CAATGACCTC
    AACACGCTTC AAGAACATAT CCGATGTTCT CATGGATTTG CCAACCCTGC TGCTAAGGAC
    AGTAATGCGT TCTTTTGCCC CCATTGCTAC ATGGGGTTTC TTACAGATTC TTCTCTGGAA
    GAGCATATTA GACAAGTCCA TTGTGATCTT AGCAGTTCCC GTTTTGGTTC CCCTGTGCTC
    GGAACCCCAA AAGATCCAGT AGTGGAAGTG TATTCTTGTT CTTACTGTAC AAATTCTCCA
    ATATTTAATA GCGTTCTTAA ACTGAACAAG CATATCAAGG AGAACCATAA GAACATTCCT
    TTGGCACTGA ATTACATCCA CAATGGAAAA AAATCCAGAG CCATGAGTCC CTTATCTCCT
    GTAACGATTG AGCAGACCTC TTTAAAAATG ATGCAGGCGG TTGGAGGTGC TCCTCCTCGT
    CCTGCAGGTG AATATATTTG TAATCAATGT GGTGCTAAGT ATACTTCTTT GGACGGTTTT
    CAGACTCATT TAAAAACACA TCTTGATACT GTCCTGCCAA AACTGACCTG CCCTCAGTGC
    AACAAGGAGT TTCCAAATCA GGAGTCTCTG CTGAAGCATG TTACCATTCA TTTTATGATC
    ACTTCAACCT ACTACATCTG TGAAAGCTGT GACAAGCAGT TTACTTCTGT GGATGACTTG
    CAGAAGCACC TACTAGACAT GCATACATTT GTATTCTTCC GCTGCACCTT GTGCCAAGAG
    GTTTTTGACT CCAAAGTCTC CATCCAACTA CACTTGGCTG TGAAGCACAG CAATGAAAAG
    AAGGTATATA GGTGTACGTC TTGCAACTGG GATTTCCGCA ATGAGACTGA CCTACAGCTT
    CACGTTAAAC ACAACCACTT GGAGAACCAA GGCAAAGTGC ACAAGTGCAT CTTCTGTGGT
    GAGTCCTTTG GTACAGAGGT GGAGCTGCAG TGCCATATTA CTACGCACAG CAAGAAGTAC
    AACTGTAAGT TCTGCAGCAA AGCTTTCCAT GCCATCATCC TGCTGGAAAA GCATTTAAGG
    GAAAAACATT GTGTTTTTGA AACAAAAACT CCAAATTGTG GAACGAATGG GGCATCTGAA
    CAGGTTCAGA AGGAAGAAGT GGAGCTCCAG ACCTTGTTGA CAAATAGCCA GGAGTCCCAT
    AACAGCCATG ATGGCAGCGA AGAGGATGTG GACACATCTG AGCCTATGTA TGGCTGTGAC
    ATTTGTGGAG CGGCTTACAC CATGGAGACT CTCCTGCAAA ACCACCAGCT TCGAGACCAC
    AACATTCGAC CTGGAGAAAG TGCCATAGTG AAGAAAAAGG CTGAACTCAT TAAAGGGAAT
    TACAAGTGTA ATGTGTGTTC TCGAACATTC TTCTCTGAAA ATGGGCTCCG AGAACACATG
    CAGACACACT TGGGACCAGT GAAACATTAC ATGTGCCCAA TCTGTGGCGA GCGGTTTCCA
    TCTCTTCTGA CTCTTACTGA ACATAAAGTC ACACATAGTA AGAGCCTGGA CACCGGAAAC
    TGCAGGATTT GCAAGATGCC TCTCCAGAGT GAGGAGGAAT TTTTAGAGCA TTGCCAGATG
    CACCCTGACT TGAGGAACTC TTTGACAGGG TTCCGCTGTG TGGTGTGCAT GCAGACTGTG
    ACCTCCACGT TGGAACTGAA AATCCACGGC ACATTCCACA TGCAGAAAAC TGGAAATGGT
    TCTGCCGTGC AGTCCACGGG GCGCGCACAG CATCTCCAGA AACTGTACAA GTGTGCTTCT
    TGCCTGAAGG AATTTCGTTC AAAACAGGAT TTAGTGAAAC TTGACATCAA TGGTCTGCCA
    TATGGTCTGT GTGCTAGCTG TGTTAATCTC AGTAAAAGTG GTAGTCCAAG TGTCAACATC
    CCATCAAGCA GTAACAGACA AGGCATGGGC CAAAATGAGA ACTTGAGTTC CATTGAGAAC
    AAAAGCAAGG CAGGGGGACT GAAGACTCGA TGTTCTAGTT GCAATGTTAA ATTTGAATCA
    GAAAGTGAAC TCCAAAACCA TATTCAGTCA ATCCACAGAG AGCTTGTTCC AGACAGCAAC
    AGTACACAGC TGAAAACACC ACAAGTATCT CCAATGCCCA GAATCAGCCC CTCCCAAACT
    GAAGAGAAGA AGACCTACCA GTGCATCAAA TGTCAGATGG TTTTCTACAA TGAATGGGAT
    ATCCAAGTTC ATGTTGCAAA CCACATGATT GATGAAGGAC TGAACCATGA ATGCAAGCTC
    TGCAATCAGA CATTTGACTC TCCTGCCAAA CTTCAGTGCC ACCTGATAGA GCACAGCTTT
    GAAGGCATGG GGGGCACCTT CAAATGTCCA GTGTGCTTTA CAGTGTTTGT CCAAGCAAAC
    AAGTTGCAGC AGCACATTTT CTCAGCCCAT GGACAAGAGG ACAAGATCTA TGACTGCACA
    CAGTGTCCAC AGAAGTTCTT CTTCCAAACA GAGCTGCAGA ATCATACGAT GACCCAGCAT
    AGCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133475.1
    CDS5..4033
    Translation

    Target ORF information:

    RefSeq Version XM_015277989.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277989.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCTTTGG GGGCCTTGCT TCCATTTTCC ATTATTATGT GGACTACCGG AGCGACAGCG 
    CAGTCCAAGA CCTTGCAGCT GTTTGCTTTT AGGATGTCTC GCCGCAAGCA AGCTAAACCG
    AGATCACTCA AAGACCCTAA CTGTAAACTA GAAGACAAGA CAGAAGATGG AGAAGTGTTA
    GACTGTAAGA AAAGGCCGGA TGAAGGGGAG GAGTTGGAAG AAGAAGCCGT GCACAGCTGT
    GACAGCTGCC TCCAGGTGTT TGAGTCGCTG AGTGATATCA CAGAACACAA GATTAATCAA
    TGTCAACTGA CAGATGGAGT GGATGTTGAG GATGACCCCA CTTGCTCATG GCCAGCATCA
    TCGCCTTCCA GCAAGGACCA GACTTCCCCA AGCCATGGAG AAGGTTGTGA TTTTGGTGAG
    GAGGAAGGAG GCCCAGGGTT GCCCTATCAG TGCCAGTTTT GTGACAAATC GTTCAGCCGC
    CTCAGCTACT TAAAGCACCA CGAGCAAAGT CACAGTGACA AACTCCCTTT CAAATGCACT
    TATTGCAGTC GTCTCTTCAA ACACAAACGA AGCAGGGACC GCCACATAAA GCTTCATACG
    GGAGACAAGA AGTATCACTG CAGTGAGTGT GATGCAGCAT TCTCAAGGAG CGATCATCTT
    AAAATTCACT TAAAGACTCA TACGTCCAAC AAGCCATATA AATGTGCCAT TTGTAGACGT
    GGATTCCTGT CATCCAGTTC GCTGCACGGC CACATGCAGG TTCACGAGAG GAACAAGGAT
    GGCTCTCAGT CTGCTTCCCG GATGGAGGAC TGGAAAATGA AGGACACTCA GAAGTGCAGT
    CAGTGTGAAG AAGGCTTTGA TTTTCCTGAA GATCTTCAGA AGCACATTGC GGAATGTCAC
    CCTGAGTGTT CTCCTAATGA AGACCGATCT GCTCTCCAGT GTGTTTACTG TCACGAACTC
    TTTGTAGAGG AAACGTCTCT GGTGAATCAC ATGGAGCAGG CTCATAATGG TGAGAAGAAG
    AATTCATGCA GCATTTGCTC TGAGAACTTT CACACTGTGG AAGAGCTGTA CAGCCACATG
    GACAGTCACC AGCAGCCAGA ATCATGCAAC CACAGCAACA GCCCATCATT GGTAACTGTT
    GGCTACACCT CAGTCTCAAG TACCACTCCA GATTCCAATC TCTCTGTGGA TAGTTCAACA
    ATGGTGGAAA CGGCTCCCCC TATACCAAAA GGCCGTGGAA GAAAGCGGGC AGCTCAGCAA
    GTGCCAGATA TCACTGGTCC TTCAAGTAAA CAGGCAAAAG TTACCTATAG CTGCATTTAT
    TGCAACAAAC AGTTATTTTC CAGCCTTGCA GTTTTGCAGA TACACCTGAA AACTATGCAT
    TTAGATAAAC CTGAACAAGC CCATATCTGT CAGTATTGTT TGGAGGTATT GCCATCTCTC
    TACAATCTGA ACGAACATCT TAAGCAAGTC CACGAAGCTC CGGATCCAGG GCTGATTGTT
    TCCACTATGC CTGCCATGGT GTACCAGTGC AACTTCTGCT CTGAAGTGTT CAATGACCTC
    AACACGCTTC AAGAACATAT CCGATGTTCT CATGGATTTG CCAACCCTGC TGCTAAGGAC
    AGTAATGCGT TCTTTTGCCC CCATTGCTAC ATGGGGTTTC TTACAGATTC TTCTCTGGAA
    GAGCATATTA GACAAGTCCA TTGTGATCTT AGCAGTTCCC GTTTTGGTTC CCCTGTGCTC
    GGAACCCCAA AAGATCCAGT AGTGGAAGTG TATTCTTGTT CTTACTGTAC AAATTCTCCA
    ATATTTAATA GCGTTCTTAA ACTGAACAAG CATATCAAGG AGAACCATAA GAACATTCCT
    TTGGCACTGA ATTACATCCA CAATGGAAAA AAATCCAGAG CCATGAGTCC CTTATCTCCT
    GTAACGATTG AGCAGACCTC TTTAAAAATG ATGCAGGCGG TTGGAGGTGC TCCTCCTCGT
    CCTGCAGGTG AATATATTTG TAATCAATGT GGTGCTAAGT ATACTTCTTT GGACGGTTTT
    CAGACTCATT TAAAAACACA TCTTGATACT GTCCTGCCAA AACTGACCTG CCCTCAGTGC
    AACAAGGAGT TTCCAAATCA GGAGTCTCTG CTGAAGCATG TTACCATTCA TTTTATGATC
    ACTTCAACCT ACTACATCTG TGAAAGCTGT GACAAGCAGT TTACTTCTGT GGATGACTTG
    CAGAAGCACC TACTAGACAT GCATACATTT GTATTCTTCC GCTGCACCTT GTGCCAAGAG
    GTTTTTGACT CCAAAGTCTC CATCCAACTA CACTTGGCTG TGAAGCACAG CAATGAAAAG
    AAGGTATATA GGTGTACGTC TTGCAACTGG GATTTCCGCA ATGAGACTGA CCTACAGCTT
    CACGTTAAAC ACAACCACTT GGAGAACCAA GGCAAAGTGC ACAAGTGCAT CTTCTGTGGT
    GAGTCCTTTG GTACAGAGGT GGAGCTGCAG TGCCATATTA CTACGCACAG CAAGAAGTAC
    AACTGTAAGT TCTGCAGCAA AGCTTTCCAT GCCATCATCC TGCTGGAAAA GCATTTAAGG
    GAAAAACATT GTGTTTTTGA AACAAAAACT CCAAATTGTG GAACGAATGG GGCATCTGAA
    CAGGTTCAGA AGGAAGAAGT GGAGCTCCAG ACCTTGTTGA CAAATAGCCA GGAGTCCCAT
    AACAGCCATG ATGGCAGCGA AGAGGATGTG GACACATCTG AGCCTATGTA TGGCTGTGAC
    ATTTGTGGAG CGGCTTACAC CATGGAGACT CTCCTGCAAA ACCACCAGCT TCGAGACCAC
    AACATTCGAC CTGGAGAAAG TGCCATAGTG AAGAAAAAGG CTGAACTCAT TAAAGGGAAT
    TACAAGTGTA ATGTGTGTTC TCGAACATTC TTCTCTGAAA ATGGGCTCCG AGAACACATG
    CAGACACACT TGGGACCAGT GAAACATTAC ATGTGCCCAA TCTGTGGCGA GCGGTTTCCA
    TCTCTTCTGA CTCTTACTGA ACATAAAGTC ACACATAGTA AGAGCCTGGA CACCGGAAAC
    TGCAGGATTT GCAAGATGCC TCTCCAGAGT GAGGAGGAAT TTTTAGAGCA TTGCCAGATG
    CACCCTGACT TGAGGAACTC TTTGACAGGG TTCCGCTGTG TGGTGTGCAT GCAGACTGTG
    ACCTCCACGT TGGAACTGAA AATCCACGGC ACATTCCACA TGCAGAAAAC TGGAAATGGT
    TCTGCCGTGC AGTCCACGGG GCGCGCACAG CATCTCCAGA AACTGTACAA GTGTGCTTCT
    TGCCTGAAGG AATTTCGTTC AAAACAGGAT TTAGTGAAAC TTGACATCAA TGGTCTGCCA
    TATGGTCTGT GTGCTAGCTG TGTTAATCTC AGTAAAAGTG GTAGTCCAAG TGTCAACATC
    CCATCAAGCA GTAACAGACA AGGCATGGGC CAAAATGAGA ACTTGAGTTC CATTGAGAAC
    AAAAGCAAGG CAGGGGGACT GAAGACTCGA TGTTCTAGTT GCAATGTTAA ATTTGAATCA
    GAAAGTGAAC TCCAAAACCA TATTCAGTCA ATCCACAGAG AGCTTGTTCC AGACAGCAAC
    AGTACACAGC TGAAAACACC ACAAGTATCT CCAATGCCCA GAATCAGCCC CTCCCAAACT
    GAAGAGAAGA AGACCTACCA GTGCATCAAA TGTCAGATGG TTTTCTACAA TGAATGGGAT
    ATCCAAGTTC ATGTTGCAAA CCACATGATT GATGAAGGAC TGAACCATGA ATGCAAGCTC
    TGCAATCAGA CATTTGACTC TCCTGCCAAA CTTCAGTGCC ACCTGATAGA GCACAGCTTT
    GAAGGCATGG GGGGCACCTT CAAATGTCCA GTGTGCTTTA CAGTGTTTGT CCAAGCAAAC
    AAGTTGCAGC AGCACATTTT CTCAGCCCAT GGACAAGAGG ACAAGATCTA TGACTGCACA
    CAGTGTCCAC AGAAGTTCTT CTTCCAAACA GAGCTGCAGA ATCATACGAT GACCCAGCAT
    AGCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32514
    Clone ID Related Accession (Same CDS sequence) XM_015277990.1 , XM_015277990.2
    Accession Version XM_015277990.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product zinc finger protein 521 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCTCGCC GCAAGCAAGC TAAACCGAGA TCACTCAAAG TAGAAGAAAA TGAAACAGAG 
    GACCTGCAGG CAGGTGTGGG ACACACAGCC GCGCAGACTG ACCCTAACTG TAAACTAGAA
    GACAAGACAG AAGATGGAGA AGTGTTAGAC TGTAAGAAAA GGCCGGATGA AGGGGAGGAG
    TTGGAAGAAG AAGCCGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTTGA GTCGCTGAGT
    GATATCACAG AACACAAGAT TAATCAATGT CAACTGACAG ATGGAGTGGA TGTTGAGGAT
    GACCCCACTT GCTCATGGCC AGCATCATCG CCTTCCAGCA AGGACCAGAC TTCCCCAAGC
    CATGGAGAAG GTTGTGATTT TGGTGAGGAG GAAGGAGGCC CAGGGTTGCC CTATCAGTGC
    CAGTTTTGTG ACAAATCGTT CAGCCGCCTC AGCTACTTAA AGCACCACGA GCAAAGTCAC
    AGTGACAAAC TCCCTTTCAA ATGCACTTAT TGCAGTCGTC TCTTCAAACA CAAACGAAGC
    AGGGACCGCC ACATAAAGCT TCATACGGGA GACAAGAAGT ATCACTGCAG TGAGTGTGAT
    GCAGCATTCT CAAGGAGCGA TCATCTTAAA ATTCACTTAA AGACTCATAC GTCCAACAAG
    CCATATAAAT GTGCCATTTG TAGACGTGGA TTCCTGTCAT CCAGTTCGCT GCACGGCCAC
    ATGCAGGTTC ACGAGAGGAA CAAGGATGGC TCTCAGTCTG CTTCCCGGAT GGAGGACTGG
    AAAATGAAGG ACACTCAGAA GTGCAGTCAG TGTGAAGAAG GCTTTGATTT TCCTGAAGAT
    CTTCAGAAGC ACATTGCGGA ATGTCACCCT GAGTGTTCTC CTAATGAAGA CCGATCTGCT
    CTCCAGTGTG TTTACTGTCA CGAACTCTTT GTAGAGGAAA CGTCTCTGGT GAATCACATG
    GAGCAGGCTC ATAATGGTGA GAAGAAGAAT TCATGCAGCA TTTGCTCTGA GAACTTTCAC
    ACTGTGGAAG AGCTGTACAG CCACATGGAC AGTCACCAGC AGCCAGAATC ATGCAACCAC
    AGCAACAGCC CATCATTGGT AACTGTTGGC TACACCTCAG TCTCAAGTAC CACTCCAGAT
    TCCAATCTCT CTGTGGATAG TTCAACAATG GTGGAAACGG CTCCCCCTAT ACCAAAAGGC
    CGTGGAAGAA AGCGGGCAGC TCAGCAAGTG CCAGATATCA CTGGTCCTTC AAGTAAACAG
    GCAAAAGTTA CCTATAGCTG CATTTATTGC AACAAACAGT TATTTTCCAG CCTTGCAGTT
    TTGCAGATAC ACCTGAAAAC TATGCATTTA GATAAACCTG AACAAGCCCA TATCTGTCAG
    TATTGTTTGG AGGTATTGCC ATCTCTCTAC AATCTGAACG AACATCTTAA GCAAGTCCAC
    GAAGCTCCGG ATCCAGGGCT GATTGTTTCC ACTATGCCTG CCATGGTGTA CCAGTGCAAC
    TTCTGCTCTG AAGTGTTCAA TGACCTCAAC ACGCTTCAAG AACATATCCG ATGTTCTCAT
    GGATTTGCCA ACCCTGCTGC TAAGGACAGT AATGCGTTCT TTTGCCCCCA TTGCTACATG
    GGGTTTCTTA CAGATTCTTC TCTGGAAGAG CATATTAGAC AAGTCCATTG TGATCTTAGC
    AGTTCCCGTT TTGGTTCCCC TGTGCTCGGA ACCCCAAAAG ATCCAGTAGT GGAAGTGTAT
    TCTTGTTCTT ACTGTACAAA TTCTCCAATA TTTAATAGCG TTCTTAAACT GAACAAGCAT
    ATCAAGGAGA ACCATAAGAA CATTCCTTTG GCACTGAATT ACATCCACAA TGGAAAAAAA
    TCCAGAGCCA TGAGTCCCTT ATCTCCTGTA ACGATTGAGC AGACCTCTTT AAAAATGATG
    CAGGCGGTTG GAGGTGCTCC TCCTCGTCCT GCAGGTGAAT ATATTTGTAA TCAATGTGGT
    GCTAAGTATA CTTCTTTGGA CGGTTTTCAG ACTCATTTAA AAACACATCT TGATACTGTC
    CTGCCAAAAC TGACCTGCCC TCAGTGCAAC AAGGAGTTTC CAAATCAGGA GTCTCTGCTG
    AAGCATGTTA CCATTCATTT TATGATCACT TCAACCTACT ACATCTGTGA AAGCTGTGAC
    AAGCAGTTTA CTTCTGTGGA TGACTTGCAG AAGCACCTAC TAGACATGCA TACATTTGTA
    TTCTTCCGCT GCACCTTGTG CCAAGAGGTT TTTGACTCCA AAGTCTCCAT CCAACTACAC
    TTGGCTGTGA AGCACAGCAA TGAAAAGAAG GTATATAGGT GTACGTCTTG CAACTGGGAT
    TTCCGCAATG AGACTGACCT ACAGCTTCAC GTTAAACACA ACCACTTGGA GAACCAAGGC
    AAAGTGCACA AGTGCATCTT CTGTGGTGAG TCCTTTGGTA CAGAGGTGGA GCTGCAGTGC
    CATATTACTA CGCACAGCAA GAAGTACAAC TGTAAGTTCT GCAGCAAAGC TTTCCATGCC
    ATCATCCTGC TGGAAAAGCA TTTAAGGGAA AAACATTGTG TTTTTGAAAC AAAAACTCCA
    AATTGTGGAA CGAATGGGGC ATCTGAACAG GTTCAGAAGG AAGAAGTGGA GCTCCAGACC
    TTGTTGACAA ATAGCCAGGA GTCCCATAAC AGCCATGATG GCAGCGAAGA GGATGTGGAC
    ACATCTGAGC CTATGTATGG CTGTGACATT TGTGGAGCGG CTTACACCAT GGAGACTCTC
    CTGCAAAACC ACCAGCTTCG AGACCACAAC ATTCGACCTG GAGAAAGTGC CATAGTGAAG
    AAAAAGGCTG AACTCATTAA AGGGAATTAC AAGTGTAATG TGTGTTCTCG AACATTCTTC
    TCTGAAAATG GGCTCCGAGA ACACATGCAG ACACACTTGG GACCAGTGAA ACATTACATG
    TGCCCAATCT GTGGCGAGCG GTTTCCATCT CTTCTGACTC TTACTGAACA TAAAGTCACA
    CATAGTAAGA GCCTGGACAC CGGAAACTGC AGGATTTGCA AGATGCCTCT CCAGAGTGAG
    GAGGAATTTT TAGAGCATTG CCAGATGCAC CCTGACTTGA GGAACTCTTT GACAGGGTTC
    CGCTGTGTGG TGTGCATGCA GACTGTGACC TCCACGTTGG AACTGAAAAT CCACGGCACA
    TTCCACATGC AGAAAACTGG AAATGGTTCT GCCGTGCAGT CCACGGGGCG CGCACAGCAT
    CTCCAGAAAC TGTACAAGTG TGCTTCTTGC CTGAAGGAAT TTCGTTCAAA ACAGGATTTA
    GTGAAACTTG ACATCAATGG TCTGCCATAT GGTCTGTGTG CTAGCTGTGT TAATCTCAGT
    AAAAGTGGTA GTCCAAGTGT CAACATCCCA TCAAGCAGTA ACAGACAAGG CATGGGCCAA
    AATGAGAACT TGAGTTCCAT TGAGAACAAA AGCAAGGCAG GGGGACTGAA GACTCGATGT
    TCTAGTTGCA ATGTTAAATT TGAATCAGAA AGTGAACTCC AAAACCATAT TCAGTCAATC
    CACAGAGAGC TTGTTCCAGA CAGCAACAGT ACACAGCTGA AAACACCACA AGTATCTCCA
    ATGCCCAGAA TCAGCCCCTC CCAAACTGAA GAGAAGAAGA CCTACCAGTG CATCAAATGT
    CAGATGGTTT TCTACAATGA ATGGGATATC CAAGTTCATG TTGCAAACCA CATGATTGAT
    GAAGGACTGA ACCATGAATG CAAGCTCTGC AATCAGACAT TTGACTCTCC TGCCAAACTT
    CAGTGCCACC TGATAGAGCA CAGCTTTGAA GGCATGGGGG GCACCTTCAA ATGTCCAGTG
    TGCTTTACAG TGTTTGTCCA AGCAAACAAG TTGCAGCAGC ACATTTTCTC AGCCCATGGA
    CAAGAGGACA AGATCTATGA CTGCACACAG TGTCCACAGA AGTTCTTCTT CCAAACAGAG
    CTGCAGAATC ATACGATGAC CCAGCATAGC AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133476.1
    CDS174..4169
    Translation

    Target ORF information:

    RefSeq Version XM_015277990.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277990.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCTCGCC GCAAGCAAGC TAAACCGAGA TCACTCAAAG TAGAAGAAAA TGAAACAGAG 
    GACCTGCAGG CAGGTGTGGG ACACACAGCC GCGCAGACTG ACCCTAACTG TAAACTAGAA
    GACAAGACAG AAGATGGAGA AGTGTTAGAC TGTAAGAAAA GGCCGGATGA AGGGGAGGAG
    TTGGAAGAAG AAGCCGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTTGA GTCGCTGAGT
    GATATCACAG AACACAAGAT TAATCAATGT CAACTGACAG ATGGAGTGGA TGTTGAGGAT
    GACCCCACTT GCTCATGGCC AGCATCATCG CCTTCCAGCA AGGACCAGAC TTCCCCAAGC
    CATGGAGAAG GTTGTGATTT TGGTGAGGAG GAAGGAGGCC CAGGGTTGCC CTATCAGTGC
    CAGTTTTGTG ACAAATCGTT CAGCCGCCTC AGCTACTTAA AGCACCACGA GCAAAGTCAC
    AGTGACAAAC TCCCTTTCAA ATGCACTTAT TGCAGTCGTC TCTTCAAACA CAAACGAAGC
    AGGGACCGCC ACATAAAGCT TCATACGGGA GACAAGAAGT ATCACTGCAG TGAGTGTGAT
    GCAGCATTCT CAAGGAGCGA TCATCTTAAA ATTCACTTAA AGACTCATAC GTCCAACAAG
    CCATATAAAT GTGCCATTTG TAGACGTGGA TTCCTGTCAT CCAGTTCGCT GCACGGCCAC
    ATGCAGGTTC ACGAGAGGAA CAAGGATGGC TCTCAGTCTG CTTCCCGGAT GGAGGACTGG
    AAAATGAAGG ACACTCAGAA GTGCAGTCAG TGTGAAGAAG GCTTTGATTT TCCTGAAGAT
    CTTCAGAAGC ACATTGCGGA ATGTCACCCT GAGTGTTCTC CTAATGAAGA CCGATCTGCT
    CTCCAGTGTG TTTACTGTCA CGAACTCTTT GTAGAGGAAA CGTCTCTGGT GAATCACATG
    GAGCAGGCTC ATAATGGTGA GAAGAAGAAT TCATGCAGCA TTTGCTCTGA GAACTTTCAC
    ACTGTGGAAG AGCTGTACAG CCACATGGAC AGTCACCAGC AGCCAGAATC ATGCAACCAC
    AGCAACAGCC CATCATTGGT AACTGTTGGC TACACCTCAG TCTCAAGTAC CACTCCAGAT
    TCCAATCTCT CTGTGGATAG TTCAACAATG GTGGAAACGG CTCCCCCTAT ACCAAAAGGC
    CGTGGAAGAA AGCGGGCAGC TCAGCAAGTG CCAGATATCA CTGGTCCTTC AAGTAAACAG
    GCAAAAGTTA CCTATAGCTG CATTTATTGC AACAAACAGT TATTTTCCAG CCTTGCAGTT
    TTGCAGATAC ACCTGAAAAC TATGCATTTA GATAAACCTG AACAAGCCCA TATCTGTCAG
    TATTGTTTGG AGGTATTGCC ATCTCTCTAC AATCTGAACG AACATCTTAA GCAAGTCCAC
    GAAGCTCCGG ATCCAGGGCT GATTGTTTCC ACTATGCCTG CCATGGTGTA CCAGTGCAAC
    TTCTGCTCTG AAGTGTTCAA TGACCTCAAC ACGCTTCAAG AACATATCCG ATGTTCTCAT
    GGATTTGCCA ACCCTGCTGC TAAGGACAGT AATGCGTTCT TTTGCCCCCA TTGCTACATG
    GGGTTTCTTA CAGATTCTTC TCTGGAAGAG CATATTAGAC AAGTCCATTG TGATCTTAGC
    AGTTCCCGTT TTGGTTCCCC TGTGCTCGGA ACCCCAAAAG ATCCAGTAGT GGAAGTGTAT
    TCTTGTTCTT ACTGTACAAA TTCTCCAATA TTTAATAGCG TTCTTAAACT GAACAAGCAT
    ATCAAGGAGA ACCATAAGAA CATTCCTTTG GCACTGAATT ACATCCACAA TGGAAAAAAA
    TCCAGAGCCA TGAGTCCCTT ATCTCCTGTA ACGATTGAGC AGACCTCTTT AAAAATGATG
    CAGGCGGTTG GAGGTGCTCC TCCTCGTCCT GCAGGTGAAT ATATTTGTAA TCAATGTGGT
    GCTAAGTATA CTTCTTTGGA CGGTTTTCAG ACTCATTTAA AAACACATCT TGATACTGTC
    CTGCCAAAAC TGACCTGCCC TCAGTGCAAC AAGGAGTTTC CAAATCAGGA GTCTCTGCTG
    AAGCATGTTA CCATTCATTT TATGATCACT TCAACCTACT ACATCTGTGA AAGCTGTGAC
    AAGCAGTTTA CTTCTGTGGA TGACTTGCAG AAGCACCTAC TAGACATGCA TACATTTGTA
    TTCTTCCGCT GCACCTTGTG CCAAGAGGTT TTTGACTCCA AAGTCTCCAT CCAACTACAC
    TTGGCTGTGA AGCACAGCAA TGAAAAGAAG GTATATAGGT GTACGTCTTG CAACTGGGAT
    TTCCGCAATG AGACTGACCT ACAGCTTCAC GTTAAACACA ACCACTTGGA GAACCAAGGC
    AAAGTGCACA AGTGCATCTT CTGTGGTGAG TCCTTTGGTA CAGAGGTGGA GCTGCAGTGC
    CATATTACTA CGCACAGCAA GAAGTACAAC TGTAAGTTCT GCAGCAAAGC TTTCCATGCC
    ATCATCCTGC TGGAAAAGCA TTTAAGGGAA AAACATTGTG TTTTTGAAAC AAAAACTCCA
    AATTGTGGAA CGAATGGGGC ATCTGAACAG GTTCAGAAGG AAGAAGTGGA GCTCCAGACC
    TTGTTGACAA ATAGCCAGGA GTCCCATAAC AGCCATGATG GCAGCGAAGA GGATGTGGAC
    ACATCTGAGC CTATGTATGG CTGTGACATT TGTGGAGCGG CTTACACCAT GGAGACTCTC
    CTGCAAAACC ACCAGCTTCG AGACCACAAC ATTCGACCTG GAGAAAGTGC CATAGTGAAG
    AAAAAGGCTG AACTCATTAA AGGGAATTAC AAGTGTAATG TGTGTTCTCG AACATTCTTC
    TCTGAAAATG GGCTCCGAGA ACACATGCAG ACACACTTGG GACCAGTGAA ACATTACATG
    TGCCCAATCT GTGGCGAGCG GTTTCCATCT CTTCTGACTC TTACTGAACA TAAAGTCACA
    CATAGTAAGA GCCTGGACAC CGGAAACTGC AGGATTTGCA AGATGCCTCT CCAGAGTGAG
    GAGGAATTTT TAGAGCATTG CCAGATGCAC CCTGACTTGA GGAACTCTTT GACAGGGTTC
    CGCTGTGTGG TGTGCATGCA GACTGTGACC TCCACGTTGG AACTGAAAAT CCACGGCACA
    TTCCACATGC AGAAAACTGG AAATGGTTCT GCCGTGCAGT CCACGGGGCG CGCACAGCAT
    CTCCAGAAAC TGTACAAGTG TGCTTCTTGC CTGAAGGAAT TTCGTTCAAA ACAGGATTTA
    GTGAAACTTG ACATCAATGG TCTGCCATAT GGTCTGTGTG CTAGCTGTGT TAATCTCAGT
    AAAAGTGGTA GTCCAAGTGT CAACATCCCA TCAAGCAGTA ACAGACAAGG CATGGGCCAA
    AATGAGAACT TGAGTTCCAT TGAGAACAAA AGCAAGGCAG GGGGACTGAA GACTCGATGT
    TCTAGTTGCA ATGTTAAATT TGAATCAGAA AGTGAACTCC AAAACCATAT TCAGTCAATC
    CACAGAGAGC TTGTTCCAGA CAGCAACAGT ACACAGCTGA AAACACCACA AGTATCTCCA
    ATGCCCAGAA TCAGCCCCTC CCAAACTGAA GAGAAGAAGA CCTACCAGTG CATCAAATGT
    CAGATGGTTT TCTACAATGA ATGGGATATC CAAGTTCATG TTGCAAACCA CATGATTGAT
    GAAGGACTGA ACCATGAATG CAAGCTCTGC AATCAGACAT TTGACTCTCC TGCCAAACTT
    CAGTGCCACC TGATAGAGCA CAGCTTTGAA GGCATGGGGG GCACCTTCAA ATGTCCAGTG
    TGCTTTACAG TGTTTGTCCA AGCAAACAAG TTGCAGCAGC ACATTTTCTC AGCCCATGGA
    CAAGAGGACA AGATCTATGA CTGCACACAG TGTCCACAGA AGTTCTTCTT CCAAACAGAG
    CTGCAGAATC ATACGATGAC CCAGCATAGC AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32514
    Clone ID Related Accession (Same CDS sequence) XM_015277990.1 , XM_015277990.2
    Accession Version XM_015277990.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product zinc finger protein 521 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277990.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCTCGCC GCAAGCAAGC TAAACCGAGA TCACTCAAAG TAGAAGAAAA TGAAACAGAG 
    GACCTGCAGG CAGGTGTGGG ACACACAGCC GCGCAGACTG ACCCTAACTG TAAACTAGAA
    GACAAGACAG AAGATGGAGA AGTGTTAGAC TGTAAGAAAA GGCCGGATGA AGGGGAGGAG
    TTGGAAGAAG AAGCCGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTTGA GTCGCTGAGT
    GATATCACAG AACACAAGAT TAATCAATGT CAACTGACAG ATGGAGTGGA TGTTGAGGAT
    GACCCCACTT GCTCATGGCC AGCATCATCG CCTTCCAGCA AGGACCAGAC TTCCCCAAGC
    CATGGAGAAG GTTGTGATTT TGGTGAGGAG GAAGGAGGCC CAGGGTTGCC CTATCAGTGC
    CAGTTTTGTG ACAAATCGTT CAGCCGCCTC AGCTACTTAA AGCACCACGA GCAAAGTCAC
    AGTGACAAAC TCCCTTTCAA ATGCACTTAT TGCAGTCGTC TCTTCAAACA CAAACGAAGC
    AGGGACCGCC ACATAAAGCT TCATACGGGA GACAAGAAGT ATCACTGCAG TGAGTGTGAT
    GCAGCATTCT CAAGGAGCGA TCATCTTAAA ATTCACTTAA AGACTCATAC GTCCAACAAG
    CCATATAAAT GTGCCATTTG TAGACGTGGA TTCCTGTCAT CCAGTTCGCT GCACGGCCAC
    ATGCAGGTTC ACGAGAGGAA CAAGGATGGC TCTCAGTCTG CTTCCCGGAT GGAGGACTGG
    AAAATGAAGG ACACTCAGAA GTGCAGTCAG TGTGAAGAAG GCTTTGATTT TCCTGAAGAT
    CTTCAGAAGC ACATTGCGGA ATGTCACCCT GAGTGTTCTC CTAATGAAGA CCGATCTGCT
    CTCCAGTGTG TTTACTGTCA CGAACTCTTT GTAGAGGAAA CGTCTCTGGT GAATCACATG
    GAGCAGGCTC ATAATGGTGA GAAGAAGAAT TCATGCAGCA TTTGCTCTGA GAACTTTCAC
    ACTGTGGAAG AGCTGTACAG CCACATGGAC AGTCACCAGC AGCCAGAATC ATGCAACCAC
    AGCAACAGCC CATCATTGGT AACTGTTGGC TACACCTCAG TCTCAAGTAC CACTCCAGAT
    TCCAATCTCT CTGTGGATAG TTCAACAATG GTGGAAACGG CTCCCCCTAT ACCAAAAGGC
    CGTGGAAGAA AGCGGGCAGC TCAGCAAGTG CCAGATATCA CTGGTCCTTC AAGTAAACAG
    GCAAAAGTTA CCTATAGCTG CATTTATTGC AACAAACAGT TATTTTCCAG CCTTGCAGTT
    TTGCAGATAC ACCTGAAAAC TATGCATTTA GATAAACCTG AACAAGCCCA TATCTGTCAG
    TATTGTTTGG AGGTATTGCC ATCTCTCTAC AATCTGAACG AACATCTTAA GCAAGTCCAC
    GAAGCTCCGG ATCCAGGGCT GATTGTTTCC ACTATGCCTG CCATGGTGTA CCAGTGCAAC
    TTCTGCTCTG AAGTGTTCAA TGACCTCAAC ACGCTTCAAG AACATATCCG ATGTTCTCAT
    GGATTTGCCA ACCCTGCTGC TAAGGACAGT AATGCGTTCT TTTGCCCCCA TTGCTACATG
    GGGTTTCTTA CAGATTCTTC TCTGGAAGAG CATATTAGAC AAGTCCATTG TGATCTTAGC
    AGTTCCCGTT TTGGTTCCCC TGTGCTCGGA ACCCCAAAAG ATCCAGTAGT GGAAGTGTAT
    TCTTGTTCTT ACTGTACAAA TTCTCCAATA TTTAATAGCG TTCTTAAACT GAACAAGCAT
    ATCAAGGAGA ACCATAAGAA CATTCCTTTG GCACTGAATT ACATCCACAA TGGAAAAAAA
    TCCAGAGCCA TGAGTCCCTT ATCTCCTGTA ACGATTGAGC AGACCTCTTT AAAAATGATG
    CAGGCGGTTG GAGGTGCTCC TCCTCGTCCT GCAGGTGAAT ATATTTGTAA TCAATGTGGT
    GCTAAGTATA CTTCTTTGGA CGGTTTTCAG ACTCATTTAA AAACACATCT TGATACTGTC
    CTGCCAAAAC TGACCTGCCC TCAGTGCAAC AAGGAGTTTC CAAATCAGGA GTCTCTGCTG
    AAGCATGTTA CCATTCATTT TATGATCACT TCAACCTACT ACATCTGTGA AAGCTGTGAC
    AAGCAGTTTA CTTCTGTGGA TGACTTGCAG AAGCACCTAC TAGACATGCA TACATTTGTA
    TTCTTCCGCT GCACCTTGTG CCAAGAGGTT TTTGACTCCA AAGTCTCCAT CCAACTACAC
    TTGGCTGTGA AGCACAGCAA TGAAAAGAAG GTATATAGGT GTACGTCTTG CAACTGGGAT
    TTCCGCAATG AGACTGACCT ACAGCTTCAC GTTAAACACA ACCACTTGGA GAACCAAGGC
    AAAGTGCACA AGTGCATCTT CTGTGGTGAG TCCTTTGGTA CAGAGGTGGA GCTGCAGTGC
    CATATTACTA CGCACAGCAA GAAGTACAAC TGTAAGTTCT GCAGCAAAGC TTTCCATGCC
    ATCATCCTGC TGGAAAAGCA TTTAAGGGAA AAACATTGTG TTTTTGAAAC AAAAACTCCA
    AATTGTGGAA CGAATGGGGC ATCTGAACAG GTTCAGAAGG AAGAAGTGGA GCTCCAGACC
    TTGTTGACAA ATAGCCAGGA GTCCCATAAC AGCCATGATG GCAGCGAAGA GGATGTGGAC
    ACATCTGAGC CTATGTATGG CTGTGACATT TGTGGAGCGG CTTACACCAT GGAGACTCTC
    CTGCAAAACC ACCAGCTTCG AGACCACAAC ATTCGACCTG GAGAAAGTGC CATAGTGAAG
    AAAAAGGCTG AACTCATTAA AGGGAATTAC AAGTGTAATG TGTGTTCTCG AACATTCTTC
    TCTGAAAATG GGCTCCGAGA ACACATGCAG ACACACTTGG GACCAGTGAA ACATTACATG
    TGCCCAATCT GTGGCGAGCG GTTTCCATCT CTTCTGACTC TTACTGAACA TAAAGTCACA
    CATAGTAAGA GCCTGGACAC CGGAAACTGC AGGATTTGCA AGATGCCTCT CCAGAGTGAG
    GAGGAATTTT TAGAGCATTG CCAGATGCAC CCTGACTTGA GGAACTCTTT GACAGGGTTC
    CGCTGTGTGG TGTGCATGCA GACTGTGACC TCCACGTTGG AACTGAAAAT CCACGGCACA
    TTCCACATGC AGAAAACTGG AAATGGTTCT GCCGTGCAGT CCACGGGGCG CGCACAGCAT
    CTCCAGAAAC TGTACAAGTG TGCTTCTTGC CTGAAGGAAT TTCGTTCAAA ACAGGATTTA
    GTGAAACTTG ACATCAATGG TCTGCCATAT GGTCTGTGTG CTAGCTGTGT TAATCTCAGT
    AAAAGTGGTA GTCCAAGTGT CAACATCCCA TCAAGCAGTA ACAGACAAGG CATGGGCCAA
    AATGAGAACT TGAGTTCCAT TGAGAACAAA AGCAAGGCAG GGGGACTGAA GACTCGATGT
    TCTAGTTGCA ATGTTAAATT TGAATCAGAA AGTGAACTCC AAAACCATAT TCAGTCAATC
    CACAGAGAGC TTGTTCCAGA CAGCAACAGT ACACAGCTGA AAACACCACA AGTATCTCCA
    ATGCCCAGAA TCAGCCCCTC CCAAACTGAA GAGAAGAAGA CCTACCAGTG CATCAAATGT
    CAGATGGTTT TCTACAATGA ATGGGATATC CAAGTTCATG TTGCAAACCA CATGATTGAT
    GAAGGACTGA ACCATGAATG CAAGCTCTGC AATCAGACAT TTGACTCTCC TGCCAAACTT
    CAGTGCCACC TGATAGAGCA CAGCTTTGAA GGCATGGGGG GCACCTTCAA ATGTCCAGTG
    TGCTTTACAG TGTTTGTCCA AGCAAACAAG TTGCAGCAGC ACATTTTCTC AGCCCATGGA
    CAAGAGGACA AGATCTATGA CTGCACACAG TGTCCACAGA AGTTCTTCTT CCAAACAGAG
    CTGCAGAATC ATACGATGAC CCAGCATAGC AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133476.1
    CDS174..4169
    Translation

    Target ORF information:

    RefSeq Version XM_015277990.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277990.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCTCGCC GCAAGCAAGC TAAACCGAGA TCACTCAAAG TAGAAGAAAA TGAAACAGAG 
    GACCTGCAGG CAGGTGTGGG ACACACAGCC GCGCAGACTG ACCCTAACTG TAAACTAGAA
    GACAAGACAG AAGATGGAGA AGTGTTAGAC TGTAAGAAAA GGCCGGATGA AGGGGAGGAG
    TTGGAAGAAG AAGCCGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTTGA GTCGCTGAGT
    GATATCACAG AACACAAGAT TAATCAATGT CAACTGACAG ATGGAGTGGA TGTTGAGGAT
    GACCCCACTT GCTCATGGCC AGCATCATCG CCTTCCAGCA AGGACCAGAC TTCCCCAAGC
    CATGGAGAAG GTTGTGATTT TGGTGAGGAG GAAGGAGGCC CAGGGTTGCC CTATCAGTGC
    CAGTTTTGTG ACAAATCGTT CAGCCGCCTC AGCTACTTAA AGCACCACGA GCAAAGTCAC
    AGTGACAAAC TCCCTTTCAA ATGCACTTAT TGCAGTCGTC TCTTCAAACA CAAACGAAGC
    AGGGACCGCC ACATAAAGCT TCATACGGGA GACAAGAAGT ATCACTGCAG TGAGTGTGAT
    GCAGCATTCT CAAGGAGCGA TCATCTTAAA ATTCACTTAA AGACTCATAC GTCCAACAAG
    CCATATAAAT GTGCCATTTG TAGACGTGGA TTCCTGTCAT CCAGTTCGCT GCACGGCCAC
    ATGCAGGTTC ACGAGAGGAA CAAGGATGGC TCTCAGTCTG CTTCCCGGAT GGAGGACTGG
    AAAATGAAGG ACACTCAGAA GTGCAGTCAG TGTGAAGAAG GCTTTGATTT TCCTGAAGAT
    CTTCAGAAGC ACATTGCGGA ATGTCACCCT GAGTGTTCTC CTAATGAAGA CCGATCTGCT
    CTCCAGTGTG TTTACTGTCA CGAACTCTTT GTAGAGGAAA CGTCTCTGGT GAATCACATG
    GAGCAGGCTC ATAATGGTGA GAAGAAGAAT TCATGCAGCA TTTGCTCTGA GAACTTTCAC
    ACTGTGGAAG AGCTGTACAG CCACATGGAC AGTCACCAGC AGCCAGAATC ATGCAACCAC
    AGCAACAGCC CATCATTGGT AACTGTTGGC TACACCTCAG TCTCAAGTAC CACTCCAGAT
    TCCAATCTCT CTGTGGATAG TTCAACAATG GTGGAAACGG CTCCCCCTAT ACCAAAAGGC
    CGTGGAAGAA AGCGGGCAGC TCAGCAAGTG CCAGATATCA CTGGTCCTTC AAGTAAACAG
    GCAAAAGTTA CCTATAGCTG CATTTATTGC AACAAACAGT TATTTTCCAG CCTTGCAGTT
    TTGCAGATAC ACCTGAAAAC TATGCATTTA GATAAACCTG AACAAGCCCA TATCTGTCAG
    TATTGTTTGG AGGTATTGCC ATCTCTCTAC AATCTGAACG AACATCTTAA GCAAGTCCAC
    GAAGCTCCGG ATCCAGGGCT GATTGTTTCC ACTATGCCTG CCATGGTGTA CCAGTGCAAC
    TTCTGCTCTG AAGTGTTCAA TGACCTCAAC ACGCTTCAAG AACATATCCG ATGTTCTCAT
    GGATTTGCCA ACCCTGCTGC TAAGGACAGT AATGCGTTCT TTTGCCCCCA TTGCTACATG
    GGGTTTCTTA CAGATTCTTC TCTGGAAGAG CATATTAGAC AAGTCCATTG TGATCTTAGC
    AGTTCCCGTT TTGGTTCCCC TGTGCTCGGA ACCCCAAAAG ATCCAGTAGT GGAAGTGTAT
    TCTTGTTCTT ACTGTACAAA TTCTCCAATA TTTAATAGCG TTCTTAAACT GAACAAGCAT
    ATCAAGGAGA ACCATAAGAA CATTCCTTTG GCACTGAATT ACATCCACAA TGGAAAAAAA
    TCCAGAGCCA TGAGTCCCTT ATCTCCTGTA ACGATTGAGC AGACCTCTTT AAAAATGATG
    CAGGCGGTTG GAGGTGCTCC TCCTCGTCCT GCAGGTGAAT ATATTTGTAA TCAATGTGGT
    GCTAAGTATA CTTCTTTGGA CGGTTTTCAG ACTCATTTAA AAACACATCT TGATACTGTC
    CTGCCAAAAC TGACCTGCCC TCAGTGCAAC AAGGAGTTTC CAAATCAGGA GTCTCTGCTG
    AAGCATGTTA CCATTCATTT TATGATCACT TCAACCTACT ACATCTGTGA AAGCTGTGAC
    AAGCAGTTTA CTTCTGTGGA TGACTTGCAG AAGCACCTAC TAGACATGCA TACATTTGTA
    TTCTTCCGCT GCACCTTGTG CCAAGAGGTT TTTGACTCCA AAGTCTCCAT CCAACTACAC
    TTGGCTGTGA AGCACAGCAA TGAAAAGAAG GTATATAGGT GTACGTCTTG CAACTGGGAT
    TTCCGCAATG AGACTGACCT ACAGCTTCAC GTTAAACACA ACCACTTGGA GAACCAAGGC
    AAAGTGCACA AGTGCATCTT CTGTGGTGAG TCCTTTGGTA CAGAGGTGGA GCTGCAGTGC
    CATATTACTA CGCACAGCAA GAAGTACAAC TGTAAGTTCT GCAGCAAAGC TTTCCATGCC
    ATCATCCTGC TGGAAAAGCA TTTAAGGGAA AAACATTGTG TTTTTGAAAC AAAAACTCCA
    AATTGTGGAA CGAATGGGGC ATCTGAACAG GTTCAGAAGG AAGAAGTGGA GCTCCAGACC
    TTGTTGACAA ATAGCCAGGA GTCCCATAAC AGCCATGATG GCAGCGAAGA GGATGTGGAC
    ACATCTGAGC CTATGTATGG CTGTGACATT TGTGGAGCGG CTTACACCAT GGAGACTCTC
    CTGCAAAACC ACCAGCTTCG AGACCACAAC ATTCGACCTG GAGAAAGTGC CATAGTGAAG
    AAAAAGGCTG AACTCATTAA AGGGAATTAC AAGTGTAATG TGTGTTCTCG AACATTCTTC
    TCTGAAAATG GGCTCCGAGA ACACATGCAG ACACACTTGG GACCAGTGAA ACATTACATG
    TGCCCAATCT GTGGCGAGCG GTTTCCATCT CTTCTGACTC TTACTGAACA TAAAGTCACA
    CATAGTAAGA GCCTGGACAC CGGAAACTGC AGGATTTGCA AGATGCCTCT CCAGAGTGAG
    GAGGAATTTT TAGAGCATTG CCAGATGCAC CCTGACTTGA GGAACTCTTT GACAGGGTTC
    CGCTGTGTGG TGTGCATGCA GACTGTGACC TCCACGTTGG AACTGAAAAT CCACGGCACA
    TTCCACATGC AGAAAACTGG AAATGGTTCT GCCGTGCAGT CCACGGGGCG CGCACAGCAT
    CTCCAGAAAC TGTACAAGTG TGCTTCTTGC CTGAAGGAAT TTCGTTCAAA ACAGGATTTA
    GTGAAACTTG ACATCAATGG TCTGCCATAT GGTCTGTGTG CTAGCTGTGT TAATCTCAGT
    AAAAGTGGTA GTCCAAGTGT CAACATCCCA TCAAGCAGTA ACAGACAAGG CATGGGCCAA
    AATGAGAACT TGAGTTCCAT TGAGAACAAA AGCAAGGCAG GGGGACTGAA GACTCGATGT
    TCTAGTTGCA ATGTTAAATT TGAATCAGAA AGTGAACTCC AAAACCATAT TCAGTCAATC
    CACAGAGAGC TTGTTCCAGA CAGCAACAGT ACACAGCTGA AAACACCACA AGTATCTCCA
    ATGCCCAGAA TCAGCCCCTC CCAAACTGAA GAGAAGAAGA CCTACCAGTG CATCAAATGT
    CAGATGGTTT TCTACAATGA ATGGGATATC CAAGTTCATG TTGCAAACCA CATGATTGAT
    GAAGGACTGA ACCATGAATG CAAGCTCTGC AATCAGACAT TTGACTCTCC TGCCAAACTT
    CAGTGCCACC TGATAGAGCA CAGCTTTGAA GGCATGGGGG GCACCTTCAA ATGTCCAGTG
    TGCTTTACAG TGTTTGTCCA AGCAAACAAG TTGCAGCAGC ACATTTTCTC AGCCCATGGA
    CAAGAGGACA AGATCTATGA CTGCACACAG TGTCCACAGA AGTTCTTCTT CCAAACAGAG
    CTGCAGAATC ATACGATGAC CCAGCATAGC AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32515
    Clone ID Related Accession (Same CDS sequence) XM_015277991.1 , XM_015277991.2
    Accession Version XM_015277991.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3936bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product zinc finger protein 521 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCTCGCC GCAAGCAAGC TAAACCGAGA TCACTCAAAG ACCCTAACTG TAAACTAGAA 
    GACAAGACAG AAGATGGAGA AGTGTTAGAC TGTAAGAAAA GGCCGGATGA AGGGGAGGAG
    TTGGAAGAAG AAGCCGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTTGA GTCGCTGAGT
    GATATCACAG AACACAAGAT TAATCAATGT CAACTGACAG ATGGAGTGGA TGTTGAGGAT
    GACCCCACTT GCTCATGGCC AGCATCATCG CCTTCCAGCA AGGACCAGAC TTCCCCAAGC
    CATGGAGAAG GTTGTGATTT TGGTGAGGAG GAAGGAGGCC CAGGGTTGCC CTATCAGTGC
    CAGTTTTGTG ACAAATCGTT CAGCCGCCTC AGCTACTTAA AGCACCACGA GCAAAGTCAC
    AGTGACAAAC TCCCTTTCAA ATGCACTTAT TGCAGTCGTC TCTTCAAACA CAAACGAAGC
    AGGGACCGCC ACATAAAGCT TCATACGGGA GACAAGAAGT ATCACTGCAG TGAGTGTGAT
    GCAGCATTCT CAAGGAGCGA TCATCTTAAA ATTCACTTAA AGACTCATAC GTCCAACAAG
    CCATATAAAT GTGCCATTTG TAGACGTGGA TTCCTGTCAT CCAGTTCGCT GCACGGCCAC
    ATGCAGGTTC ACGAGAGGAA CAAGGATGGC TCTCAGTCTG CTTCCCGGAT GGAGGACTGG
    AAAATGAAGG ACACTCAGAA GTGCAGTCAG TGTGAAGAAG GCTTTGATTT TCCTGAAGAT
    CTTCAGAAGC ACATTGCGGA ATGTCACCCT GAGTGTTCTC CTAATGAAGA CCGATCTGCT
    CTCCAGTGTG TTTACTGTCA CGAACTCTTT GTAGAGGAAA CGTCTCTGGT GAATCACATG
    GAGCAGGCTC ATAATGGTGA GAAGAAGAAT TCATGCAGCA TTTGCTCTGA GAACTTTCAC
    ACTGTGGAAG AGCTGTACAG CCACATGGAC AGTCACCAGC AGCCAGAATC ATGCAACCAC
    AGCAACAGCC CATCATTGGT AACTGTTGGC TACACCTCAG TCTCAAGTAC CACTCCAGAT
    TCCAATCTCT CTGTGGATAG TTCAACAATG GTGGAAACGG CTCCCCCTAT ACCAAAAGGC
    CGTGGAAGAA AGCGGGCAGC TCAGCAAGTG CCAGATATCA CTGGTCCTTC AAGTAAACAG
    GCAAAAGTTA CCTATAGCTG CATTTATTGC AACAAACAGT TATTTTCCAG CCTTGCAGTT
    TTGCAGATAC ACCTGAAAAC TATGCATTTA GATAAACCTG AACAAGCCCA TATCTGTCAG
    TATTGTTTGG AGGTATTGCC ATCTCTCTAC AATCTGAACG AACATCTTAA GCAAGTCCAC
    GAAGCTCCGG ATCCAGGGCT GATTGTTTCC ACTATGCCTG CCATGGTGTA CCAGTGCAAC
    TTCTGCTCTG AAGTGTTCAA TGACCTCAAC ACGCTTCAAG AACATATCCG ATGTTCTCAT
    GGATTTGCCA ACCCTGCTGC TAAGGACAGT AATGCGTTCT TTTGCCCCCA TTGCTACATG
    GGGTTTCTTA CAGATTCTTC TCTGGAAGAG CATATTAGAC AAGTCCATTG TGATCTTAGC
    AGTTCCCGTT TTGGTTCCCC TGTGCTCGGA ACCCCAAAAG ATCCAGTAGT GGAAGTGTAT
    TCTTGTTCTT ACTGTACAAA TTCTCCAATA TTTAATAGCG TTCTTAAACT GAACAAGCAT
    ATCAAGGAGA ACCATAAGAA CATTCCTTTG GCACTGAATT ACATCCACAA TGGAAAAAAA
    TCCAGAGCCA TGAGTCCCTT ATCTCCTGTA ACGATTGAGC AGACCTCTTT AAAAATGATG
    CAGGCGGTTG GAGGTGCTCC TCCTCGTCCT GCAGGTGAAT ATATTTGTAA TCAATGTGGT
    GCTAAGTATA CTTCTTTGGA CGGTTTTCAG ACTCATTTAA AAACACATCT TGATACTGTC
    CTGCCAAAAC TGACCTGCCC TCAGTGCAAC AAGGAGTTTC CAAATCAGGA GTCTCTGCTG
    AAGCATGTTA CCATTCATTT TATGATCACT TCAACCTACT ACATCTGTGA AAGCTGTGAC
    AAGCAGTTTA CTTCTGTGGA TGACTTGCAG AAGCACCTAC TAGACATGCA TACATTTGTA
    TTCTTCCGCT GCACCTTGTG CCAAGAGGTT TTTGACTCCA AAGTCTCCAT CCAACTACAC
    TTGGCTGTGA AGCACAGCAA TGAAAAGAAG GTATATAGGT GTACGTCTTG CAACTGGGAT
    TTCCGCAATG AGACTGACCT ACAGCTTCAC GTTAAACACA ACCACTTGGA GAACCAAGGC
    AAAGTGCACA AGTGCATCTT CTGTGGTGAG TCCTTTGGTA CAGAGGTGGA GCTGCAGTGC
    CATATTACTA CGCACAGCAA GAAGTACAAC TGTAAGTTCT GCAGCAAAGC TTTCCATGCC
    ATCATCCTGC TGGAAAAGCA TTTAAGGGAA AAACATTGTG TTTTTGAAAC AAAAACTCCA
    AATTGTGGAA CGAATGGGGC ATCTGAACAG GTTCAGAAGG AAGAAGTGGA GCTCCAGACC
    TTGTTGACAA ATAGCCAGGA GTCCCATAAC AGCCATGATG GCAGCGAAGA GGATGTGGAC
    ACATCTGAGC CTATGTATGG CTGTGACATT TGTGGAGCGG CTTACACCAT GGAGACTCTC
    CTGCAAAACC ACCAGCTTCG AGACCACAAC ATTCGACCTG GAGAAAGTGC CATAGTGAAG
    AAAAAGGCTG AACTCATTAA AGGGAATTAC AAGTGTAATG TGTGTTCTCG AACATTCTTC
    TCTGAAAATG GGCTCCGAGA ACACATGCAG ACACACTTGG GACCAGTGAA ACATTACATG
    TGCCCAATCT GTGGCGAGCG GTTTCCATCT CTTCTGACTC TTACTGAACA TAAAGTCACA
    CATAGTAAGA GCCTGGACAC CGGAAACTGC AGGATTTGCA AGATGCCTCT CCAGAGTGAG
    GAGGAATTTT TAGAGCATTG CCAGATGCAC CCTGACTTGA GGAACTCTTT GACAGGGTTC
    CGCTGTGTGG TGTGCATGCA GACTGTGACC TCCACGTTGG AACTGAAAAT CCACGGCACA
    TTCCACATGC AGAAAACTGG AAATGGTTCT GCCGTGCAGT CCACGGGGCG CGCACAGCAT
    CTCCAGAAAC TGTACAAGTG TGCTTCTTGC CTGAAGGAAT TTCGTTCAAA ACAGGATTTA
    GTGAAACTTG ACATCAATGG TCTGCCATAT GGTCTGTGTG CTAGCTGTGT TAATCTCAGT
    AAAAGTGGTA GTCCAAGTGT CAACATCCCA TCAAGCAGTA ACAGACAAGG CATGGGCCAA
    AATGAGAACT TGAGTTCCAT TGAGAACAAA AGCAAGGCAG GGGGACTGAA GACTCGATGT
    TCTAGTTGCA ATGTTAAATT TGAATCAGAA AGTGAACTCC AAAACCATAT TCAGTCAATC
    CACAGAGAGC TTGTTCCAGA CAGCAACAGT ACACAGCTGA AAACACCACA AGTATCTCCA
    ATGCCCAGAA TCAGCCCCTC CCAAACTGAA GAGAAGAAGA CCTACCAGTG CATCAAATGT
    CAGATGGTTT TCTACAATGA ATGGGATATC CAAGTTCATG TTGCAAACCA CATGATTGAT
    GAAGGACTGA ACCATGAATG CAAGCTCTGC AATCAGACAT TTGACTCTCC TGCCAAACTT
    CAGTGCCACC TGATAGAGCA CAGCTTTGAA GGCATGGGGG GCACCTTCAA ATGTCCAGTG
    TGCTTTACAG TGTTTGTCCA AGCAAACAAG TTGCAGCAGC ACATTTTCTC AGCCCATGGA
    CAAGAGGACA AGATCTATGA CTGCACACAG TGTCCACAGA AGTTCTTCTT CCAAACAGAG
    CTGCAGAATC ATACGATGAC CCAGCATAGC AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133477.1
    CDS174..4109
    Translation

    Target ORF information:

    RefSeq Version XM_015277991.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277991.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCTCGCC GCAAGCAAGC TAAACCGAGA TCACTCAAAG ACCCTAACTG TAAACTAGAA 
    GACAAGACAG AAGATGGAGA AGTGTTAGAC TGTAAGAAAA GGCCGGATGA AGGGGAGGAG
    TTGGAAGAAG AAGCCGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTTGA GTCGCTGAGT
    GATATCACAG AACACAAGAT TAATCAATGT CAACTGACAG ATGGAGTGGA TGTTGAGGAT
    GACCCCACTT GCTCATGGCC AGCATCATCG CCTTCCAGCA AGGACCAGAC TTCCCCAAGC
    CATGGAGAAG GTTGTGATTT TGGTGAGGAG GAAGGAGGCC CAGGGTTGCC CTATCAGTGC
    CAGTTTTGTG ACAAATCGTT CAGCCGCCTC AGCTACTTAA AGCACCACGA GCAAAGTCAC
    AGTGACAAAC TCCCTTTCAA ATGCACTTAT TGCAGTCGTC TCTTCAAACA CAAACGAAGC
    AGGGACCGCC ACATAAAGCT TCATACGGGA GACAAGAAGT ATCACTGCAG TGAGTGTGAT
    GCAGCATTCT CAAGGAGCGA TCATCTTAAA ATTCACTTAA AGACTCATAC GTCCAACAAG
    CCATATAAAT GTGCCATTTG TAGACGTGGA TTCCTGTCAT CCAGTTCGCT GCACGGCCAC
    ATGCAGGTTC ACGAGAGGAA CAAGGATGGC TCTCAGTCTG CTTCCCGGAT GGAGGACTGG
    AAAATGAAGG ACACTCAGAA GTGCAGTCAG TGTGAAGAAG GCTTTGATTT TCCTGAAGAT
    CTTCAGAAGC ACATTGCGGA ATGTCACCCT GAGTGTTCTC CTAATGAAGA CCGATCTGCT
    CTCCAGTGTG TTTACTGTCA CGAACTCTTT GTAGAGGAAA CGTCTCTGGT GAATCACATG
    GAGCAGGCTC ATAATGGTGA GAAGAAGAAT TCATGCAGCA TTTGCTCTGA GAACTTTCAC
    ACTGTGGAAG AGCTGTACAG CCACATGGAC AGTCACCAGC AGCCAGAATC ATGCAACCAC
    AGCAACAGCC CATCATTGGT AACTGTTGGC TACACCTCAG TCTCAAGTAC CACTCCAGAT
    TCCAATCTCT CTGTGGATAG TTCAACAATG GTGGAAACGG CTCCCCCTAT ACCAAAAGGC
    CGTGGAAGAA AGCGGGCAGC TCAGCAAGTG CCAGATATCA CTGGTCCTTC AAGTAAACAG
    GCAAAAGTTA CCTATAGCTG CATTTATTGC AACAAACAGT TATTTTCCAG CCTTGCAGTT
    TTGCAGATAC ACCTGAAAAC TATGCATTTA GATAAACCTG AACAAGCCCA TATCTGTCAG
    TATTGTTTGG AGGTATTGCC ATCTCTCTAC AATCTGAACG AACATCTTAA GCAAGTCCAC
    GAAGCTCCGG ATCCAGGGCT GATTGTTTCC ACTATGCCTG CCATGGTGTA CCAGTGCAAC
    TTCTGCTCTG AAGTGTTCAA TGACCTCAAC ACGCTTCAAG AACATATCCG ATGTTCTCAT
    GGATTTGCCA ACCCTGCTGC TAAGGACAGT AATGCGTTCT TTTGCCCCCA TTGCTACATG
    GGGTTTCTTA CAGATTCTTC TCTGGAAGAG CATATTAGAC AAGTCCATTG TGATCTTAGC
    AGTTCCCGTT TTGGTTCCCC TGTGCTCGGA ACCCCAAAAG ATCCAGTAGT GGAAGTGTAT
    TCTTGTTCTT ACTGTACAAA TTCTCCAATA TTTAATAGCG TTCTTAAACT GAACAAGCAT
    ATCAAGGAGA ACCATAAGAA CATTCCTTTG GCACTGAATT ACATCCACAA TGGAAAAAAA
    TCCAGAGCCA TGAGTCCCTT ATCTCCTGTA ACGATTGAGC AGACCTCTTT AAAAATGATG
    CAGGCGGTTG GAGGTGCTCC TCCTCGTCCT GCAGGTGAAT ATATTTGTAA TCAATGTGGT
    GCTAAGTATA CTTCTTTGGA CGGTTTTCAG ACTCATTTAA AAACACATCT TGATACTGTC
    CTGCCAAAAC TGACCTGCCC TCAGTGCAAC AAGGAGTTTC CAAATCAGGA GTCTCTGCTG
    AAGCATGTTA CCATTCATTT TATGATCACT TCAACCTACT ACATCTGTGA AAGCTGTGAC
    AAGCAGTTTA CTTCTGTGGA TGACTTGCAG AAGCACCTAC TAGACATGCA TACATTTGTA
    TTCTTCCGCT GCACCTTGTG CCAAGAGGTT TTTGACTCCA AAGTCTCCAT CCAACTACAC
    TTGGCTGTGA AGCACAGCAA TGAAAAGAAG GTATATAGGT GTACGTCTTG CAACTGGGAT
    TTCCGCAATG AGACTGACCT ACAGCTTCAC GTTAAACACA ACCACTTGGA GAACCAAGGC
    AAAGTGCACA AGTGCATCTT CTGTGGTGAG TCCTTTGGTA CAGAGGTGGA GCTGCAGTGC
    CATATTACTA CGCACAGCAA GAAGTACAAC TGTAAGTTCT GCAGCAAAGC TTTCCATGCC
    ATCATCCTGC TGGAAAAGCA TTTAAGGGAA AAACATTGTG TTTTTGAAAC AAAAACTCCA
    AATTGTGGAA CGAATGGGGC ATCTGAACAG GTTCAGAAGG AAGAAGTGGA GCTCCAGACC
    TTGTTGACAA ATAGCCAGGA GTCCCATAAC AGCCATGATG GCAGCGAAGA GGATGTGGAC
    ACATCTGAGC CTATGTATGG CTGTGACATT TGTGGAGCGG CTTACACCAT GGAGACTCTC
    CTGCAAAACC ACCAGCTTCG AGACCACAAC ATTCGACCTG GAGAAAGTGC CATAGTGAAG
    AAAAAGGCTG AACTCATTAA AGGGAATTAC AAGTGTAATG TGTGTTCTCG AACATTCTTC
    TCTGAAAATG GGCTCCGAGA ACACATGCAG ACACACTTGG GACCAGTGAA ACATTACATG
    TGCCCAATCT GTGGCGAGCG GTTTCCATCT CTTCTGACTC TTACTGAACA TAAAGTCACA
    CATAGTAAGA GCCTGGACAC CGGAAACTGC AGGATTTGCA AGATGCCTCT CCAGAGTGAG
    GAGGAATTTT TAGAGCATTG CCAGATGCAC CCTGACTTGA GGAACTCTTT GACAGGGTTC
    CGCTGTGTGG TGTGCATGCA GACTGTGACC TCCACGTTGG AACTGAAAAT CCACGGCACA
    TTCCACATGC AGAAAACTGG AAATGGTTCT GCCGTGCAGT CCACGGGGCG CGCACAGCAT
    CTCCAGAAAC TGTACAAGTG TGCTTCTTGC CTGAAGGAAT TTCGTTCAAA ACAGGATTTA
    GTGAAACTTG ACATCAATGG TCTGCCATAT GGTCTGTGTG CTAGCTGTGT TAATCTCAGT
    AAAAGTGGTA GTCCAAGTGT CAACATCCCA TCAAGCAGTA ACAGACAAGG CATGGGCCAA
    AATGAGAACT TGAGTTCCAT TGAGAACAAA AGCAAGGCAG GGGGACTGAA GACTCGATGT
    TCTAGTTGCA ATGTTAAATT TGAATCAGAA AGTGAACTCC AAAACCATAT TCAGTCAATC
    CACAGAGAGC TTGTTCCAGA CAGCAACAGT ACACAGCTGA AAACACCACA AGTATCTCCA
    ATGCCCAGAA TCAGCCCCTC CCAAACTGAA GAGAAGAAGA CCTACCAGTG CATCAAATGT
    CAGATGGTTT TCTACAATGA ATGGGATATC CAAGTTCATG TTGCAAACCA CATGATTGAT
    GAAGGACTGA ACCATGAATG CAAGCTCTGC AATCAGACAT TTGACTCTCC TGCCAAACTT
    CAGTGCCACC TGATAGAGCA CAGCTTTGAA GGCATGGGGG GCACCTTCAA ATGTCCAGTG
    TGCTTTACAG TGTTTGTCCA AGCAAACAAG TTGCAGCAGC ACATTTTCTC AGCCCATGGA
    CAAGAGGACA AGATCTATGA CTGCACACAG TGTCCACAGA AGTTCTTCTT CCAAACAGAG
    CTGCAGAATC ATACGATGAC CCAGCATAGC AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32515
    Clone ID Related Accession (Same CDS sequence) XM_015277991.1 , XM_015277991.2
    Accession Version XM_015277991.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3936bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product zinc finger protein 521 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277991.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCTCGCC GCAAGCAAGC TAAACCGAGA TCACTCAAAG ACCCTAACTG TAAACTAGAA 
    GACAAGACAG AAGATGGAGA AGTGTTAGAC TGTAAGAAAA GGCCGGATGA AGGGGAGGAG
    TTGGAAGAAG AAGCCGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTTGA GTCGCTGAGT
    GATATCACAG AACACAAGAT TAATCAATGT CAACTGACAG ATGGAGTGGA TGTTGAGGAT
    GACCCCACTT GCTCATGGCC AGCATCATCG CCTTCCAGCA AGGACCAGAC TTCCCCAAGC
    CATGGAGAAG GTTGTGATTT TGGTGAGGAG GAAGGAGGCC CAGGGTTGCC CTATCAGTGC
    CAGTTTTGTG ACAAATCGTT CAGCCGCCTC AGCTACTTAA AGCACCACGA GCAAAGTCAC
    AGTGACAAAC TCCCTTTCAA ATGCACTTAT TGCAGTCGTC TCTTCAAACA CAAACGAAGC
    AGGGACCGCC ACATAAAGCT TCATACGGGA GACAAGAAGT ATCACTGCAG TGAGTGTGAT
    GCAGCATTCT CAAGGAGCGA TCATCTTAAA ATTCACTTAA AGACTCATAC GTCCAACAAG
    CCATATAAAT GTGCCATTTG TAGACGTGGA TTCCTGTCAT CCAGTTCGCT GCACGGCCAC
    ATGCAGGTTC ACGAGAGGAA CAAGGATGGC TCTCAGTCTG CTTCCCGGAT GGAGGACTGG
    AAAATGAAGG ACACTCAGAA GTGCAGTCAG TGTGAAGAAG GCTTTGATTT TCCTGAAGAT
    CTTCAGAAGC ACATTGCGGA ATGTCACCCT GAGTGTTCTC CTAATGAAGA CCGATCTGCT
    CTCCAGTGTG TTTACTGTCA CGAACTCTTT GTAGAGGAAA CGTCTCTGGT GAATCACATG
    GAGCAGGCTC ATAATGGTGA GAAGAAGAAT TCATGCAGCA TTTGCTCTGA GAACTTTCAC
    ACTGTGGAAG AGCTGTACAG CCACATGGAC AGTCACCAGC AGCCAGAATC ATGCAACCAC
    AGCAACAGCC CATCATTGGT AACTGTTGGC TACACCTCAG TCTCAAGTAC CACTCCAGAT
    TCCAATCTCT CTGTGGATAG TTCAACAATG GTGGAAACGG CTCCCCCTAT ACCAAAAGGC
    CGTGGAAGAA AGCGGGCAGC TCAGCAAGTG CCAGATATCA CTGGTCCTTC AAGTAAACAG
    GCAAAAGTTA CCTATAGCTG CATTTATTGC AACAAACAGT TATTTTCCAG CCTTGCAGTT
    TTGCAGATAC ACCTGAAAAC TATGCATTTA GATAAACCTG AACAAGCCCA TATCTGTCAG
    TATTGTTTGG AGGTATTGCC ATCTCTCTAC AATCTGAACG AACATCTTAA GCAAGTCCAC
    GAAGCTCCGG ATCCAGGGCT GATTGTTTCC ACTATGCCTG CCATGGTGTA CCAGTGCAAC
    TTCTGCTCTG AAGTGTTCAA TGACCTCAAC ACGCTTCAAG AACATATCCG ATGTTCTCAT
    GGATTTGCCA ACCCTGCTGC TAAGGACAGT AATGCGTTCT TTTGCCCCCA TTGCTACATG
    GGGTTTCTTA CAGATTCTTC TCTGGAAGAG CATATTAGAC AAGTCCATTG TGATCTTAGC
    AGTTCCCGTT TTGGTTCCCC TGTGCTCGGA ACCCCAAAAG ATCCAGTAGT GGAAGTGTAT
    TCTTGTTCTT ACTGTACAAA TTCTCCAATA TTTAATAGCG TTCTTAAACT GAACAAGCAT
    ATCAAGGAGA ACCATAAGAA CATTCCTTTG GCACTGAATT ACATCCACAA TGGAAAAAAA
    TCCAGAGCCA TGAGTCCCTT ATCTCCTGTA ACGATTGAGC AGACCTCTTT AAAAATGATG
    CAGGCGGTTG GAGGTGCTCC TCCTCGTCCT GCAGGTGAAT ATATTTGTAA TCAATGTGGT
    GCTAAGTATA CTTCTTTGGA CGGTTTTCAG ACTCATTTAA AAACACATCT TGATACTGTC
    CTGCCAAAAC TGACCTGCCC TCAGTGCAAC AAGGAGTTTC CAAATCAGGA GTCTCTGCTG
    AAGCATGTTA CCATTCATTT TATGATCACT TCAACCTACT ACATCTGTGA AAGCTGTGAC
    AAGCAGTTTA CTTCTGTGGA TGACTTGCAG AAGCACCTAC TAGACATGCA TACATTTGTA
    TTCTTCCGCT GCACCTTGTG CCAAGAGGTT TTTGACTCCA AAGTCTCCAT CCAACTACAC
    TTGGCTGTGA AGCACAGCAA TGAAAAGAAG GTATATAGGT GTACGTCTTG CAACTGGGAT
    TTCCGCAATG AGACTGACCT ACAGCTTCAC GTTAAACACA ACCACTTGGA GAACCAAGGC
    AAAGTGCACA AGTGCATCTT CTGTGGTGAG TCCTTTGGTA CAGAGGTGGA GCTGCAGTGC
    CATATTACTA CGCACAGCAA GAAGTACAAC TGTAAGTTCT GCAGCAAAGC TTTCCATGCC
    ATCATCCTGC TGGAAAAGCA TTTAAGGGAA AAACATTGTG TTTTTGAAAC AAAAACTCCA
    AATTGTGGAA CGAATGGGGC ATCTGAACAG GTTCAGAAGG AAGAAGTGGA GCTCCAGACC
    TTGTTGACAA ATAGCCAGGA GTCCCATAAC AGCCATGATG GCAGCGAAGA GGATGTGGAC
    ACATCTGAGC CTATGTATGG CTGTGACATT TGTGGAGCGG CTTACACCAT GGAGACTCTC
    CTGCAAAACC ACCAGCTTCG AGACCACAAC ATTCGACCTG GAGAAAGTGC CATAGTGAAG
    AAAAAGGCTG AACTCATTAA AGGGAATTAC AAGTGTAATG TGTGTTCTCG AACATTCTTC
    TCTGAAAATG GGCTCCGAGA ACACATGCAG ACACACTTGG GACCAGTGAA ACATTACATG
    TGCCCAATCT GTGGCGAGCG GTTTCCATCT CTTCTGACTC TTACTGAACA TAAAGTCACA
    CATAGTAAGA GCCTGGACAC CGGAAACTGC AGGATTTGCA AGATGCCTCT CCAGAGTGAG
    GAGGAATTTT TAGAGCATTG CCAGATGCAC CCTGACTTGA GGAACTCTTT GACAGGGTTC
    CGCTGTGTGG TGTGCATGCA GACTGTGACC TCCACGTTGG AACTGAAAAT CCACGGCACA
    TTCCACATGC AGAAAACTGG AAATGGTTCT GCCGTGCAGT CCACGGGGCG CGCACAGCAT
    CTCCAGAAAC TGTACAAGTG TGCTTCTTGC CTGAAGGAAT TTCGTTCAAA ACAGGATTTA
    GTGAAACTTG ACATCAATGG TCTGCCATAT GGTCTGTGTG CTAGCTGTGT TAATCTCAGT
    AAAAGTGGTA GTCCAAGTGT CAACATCCCA TCAAGCAGTA ACAGACAAGG CATGGGCCAA
    AATGAGAACT TGAGTTCCAT TGAGAACAAA AGCAAGGCAG GGGGACTGAA GACTCGATGT
    TCTAGTTGCA ATGTTAAATT TGAATCAGAA AGTGAACTCC AAAACCATAT TCAGTCAATC
    CACAGAGAGC TTGTTCCAGA CAGCAACAGT ACACAGCTGA AAACACCACA AGTATCTCCA
    ATGCCCAGAA TCAGCCCCTC CCAAACTGAA GAGAAGAAGA CCTACCAGTG CATCAAATGT
    CAGATGGTTT TCTACAATGA ATGGGATATC CAAGTTCATG TTGCAAACCA CATGATTGAT
    GAAGGACTGA ACCATGAATG CAAGCTCTGC AATCAGACAT TTGACTCTCC TGCCAAACTT
    CAGTGCCACC TGATAGAGCA CAGCTTTGAA GGCATGGGGG GCACCTTCAA ATGTCCAGTG
    TGCTTTACAG TGTTTGTCCA AGCAAACAAG TTGCAGCAGC ACATTTTCTC AGCCCATGGA
    CAAGAGGACA AGATCTATGA CTGCACACAG TGTCCACAGA AGTTCTTCTT CCAAACAGAG
    CTGCAGAATC ATACGATGAC CCAGCATAGC AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133477.1
    CDS174..4109
    Translation

    Target ORF information:

    RefSeq Version XM_015277991.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277991.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCTCGCC GCAAGCAAGC TAAACCGAGA TCACTCAAAG ACCCTAACTG TAAACTAGAA 
    GACAAGACAG AAGATGGAGA AGTGTTAGAC TGTAAGAAAA GGCCGGATGA AGGGGAGGAG
    TTGGAAGAAG AAGCCGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTTGA GTCGCTGAGT
    GATATCACAG AACACAAGAT TAATCAATGT CAACTGACAG ATGGAGTGGA TGTTGAGGAT
    GACCCCACTT GCTCATGGCC AGCATCATCG CCTTCCAGCA AGGACCAGAC TTCCCCAAGC
    CATGGAGAAG GTTGTGATTT TGGTGAGGAG GAAGGAGGCC CAGGGTTGCC CTATCAGTGC
    CAGTTTTGTG ACAAATCGTT CAGCCGCCTC AGCTACTTAA AGCACCACGA GCAAAGTCAC
    AGTGACAAAC TCCCTTTCAA ATGCACTTAT TGCAGTCGTC TCTTCAAACA CAAACGAAGC
    AGGGACCGCC ACATAAAGCT TCATACGGGA GACAAGAAGT ATCACTGCAG TGAGTGTGAT
    GCAGCATTCT CAAGGAGCGA TCATCTTAAA ATTCACTTAA AGACTCATAC GTCCAACAAG
    CCATATAAAT GTGCCATTTG TAGACGTGGA TTCCTGTCAT CCAGTTCGCT GCACGGCCAC
    ATGCAGGTTC ACGAGAGGAA CAAGGATGGC TCTCAGTCTG CTTCCCGGAT GGAGGACTGG
    AAAATGAAGG ACACTCAGAA GTGCAGTCAG TGTGAAGAAG GCTTTGATTT TCCTGAAGAT
    CTTCAGAAGC ACATTGCGGA ATGTCACCCT GAGTGTTCTC CTAATGAAGA CCGATCTGCT
    CTCCAGTGTG TTTACTGTCA CGAACTCTTT GTAGAGGAAA CGTCTCTGGT GAATCACATG
    GAGCAGGCTC ATAATGGTGA GAAGAAGAAT TCATGCAGCA TTTGCTCTGA GAACTTTCAC
    ACTGTGGAAG AGCTGTACAG CCACATGGAC AGTCACCAGC AGCCAGAATC ATGCAACCAC
    AGCAACAGCC CATCATTGGT AACTGTTGGC TACACCTCAG TCTCAAGTAC CACTCCAGAT
    TCCAATCTCT CTGTGGATAG TTCAACAATG GTGGAAACGG CTCCCCCTAT ACCAAAAGGC
    CGTGGAAGAA AGCGGGCAGC TCAGCAAGTG CCAGATATCA CTGGTCCTTC AAGTAAACAG
    GCAAAAGTTA CCTATAGCTG CATTTATTGC AACAAACAGT TATTTTCCAG CCTTGCAGTT
    TTGCAGATAC ACCTGAAAAC TATGCATTTA GATAAACCTG AACAAGCCCA TATCTGTCAG
    TATTGTTTGG AGGTATTGCC ATCTCTCTAC AATCTGAACG AACATCTTAA GCAAGTCCAC
    GAAGCTCCGG ATCCAGGGCT GATTGTTTCC ACTATGCCTG CCATGGTGTA CCAGTGCAAC
    TTCTGCTCTG AAGTGTTCAA TGACCTCAAC ACGCTTCAAG AACATATCCG ATGTTCTCAT
    GGATTTGCCA ACCCTGCTGC TAAGGACAGT AATGCGTTCT TTTGCCCCCA TTGCTACATG
    GGGTTTCTTA CAGATTCTTC TCTGGAAGAG CATATTAGAC AAGTCCATTG TGATCTTAGC
    AGTTCCCGTT TTGGTTCCCC TGTGCTCGGA ACCCCAAAAG ATCCAGTAGT GGAAGTGTAT
    TCTTGTTCTT ACTGTACAAA TTCTCCAATA TTTAATAGCG TTCTTAAACT GAACAAGCAT
    ATCAAGGAGA ACCATAAGAA CATTCCTTTG GCACTGAATT ACATCCACAA TGGAAAAAAA
    TCCAGAGCCA TGAGTCCCTT ATCTCCTGTA ACGATTGAGC AGACCTCTTT AAAAATGATG
    CAGGCGGTTG GAGGTGCTCC TCCTCGTCCT GCAGGTGAAT ATATTTGTAA TCAATGTGGT
    GCTAAGTATA CTTCTTTGGA CGGTTTTCAG ACTCATTTAA AAACACATCT TGATACTGTC
    CTGCCAAAAC TGACCTGCCC TCAGTGCAAC AAGGAGTTTC CAAATCAGGA GTCTCTGCTG
    AAGCATGTTA CCATTCATTT TATGATCACT TCAACCTACT ACATCTGTGA AAGCTGTGAC
    AAGCAGTTTA CTTCTGTGGA TGACTTGCAG AAGCACCTAC TAGACATGCA TACATTTGTA
    TTCTTCCGCT GCACCTTGTG CCAAGAGGTT TTTGACTCCA AAGTCTCCAT CCAACTACAC
    TTGGCTGTGA AGCACAGCAA TGAAAAGAAG GTATATAGGT GTACGTCTTG CAACTGGGAT
    TTCCGCAATG AGACTGACCT ACAGCTTCAC GTTAAACACA ACCACTTGGA GAACCAAGGC
    AAAGTGCACA AGTGCATCTT CTGTGGTGAG TCCTTTGGTA CAGAGGTGGA GCTGCAGTGC
    CATATTACTA CGCACAGCAA GAAGTACAAC TGTAAGTTCT GCAGCAAAGC TTTCCATGCC
    ATCATCCTGC TGGAAAAGCA TTTAAGGGAA AAACATTGTG TTTTTGAAAC AAAAACTCCA
    AATTGTGGAA CGAATGGGGC ATCTGAACAG GTTCAGAAGG AAGAAGTGGA GCTCCAGACC
    TTGTTGACAA ATAGCCAGGA GTCCCATAAC AGCCATGATG GCAGCGAAGA GGATGTGGAC
    ACATCTGAGC CTATGTATGG CTGTGACATT TGTGGAGCGG CTTACACCAT GGAGACTCTC
    CTGCAAAACC ACCAGCTTCG AGACCACAAC ATTCGACCTG GAGAAAGTGC CATAGTGAAG
    AAAAAGGCTG AACTCATTAA AGGGAATTAC AAGTGTAATG TGTGTTCTCG AACATTCTTC
    TCTGAAAATG GGCTCCGAGA ACACATGCAG ACACACTTGG GACCAGTGAA ACATTACATG
    TGCCCAATCT GTGGCGAGCG GTTTCCATCT CTTCTGACTC TTACTGAACA TAAAGTCACA
    CATAGTAAGA GCCTGGACAC CGGAAACTGC AGGATTTGCA AGATGCCTCT CCAGAGTGAG
    GAGGAATTTT TAGAGCATTG CCAGATGCAC CCTGACTTGA GGAACTCTTT GACAGGGTTC
    CGCTGTGTGG TGTGCATGCA GACTGTGACC TCCACGTTGG AACTGAAAAT CCACGGCACA
    TTCCACATGC AGAAAACTGG AAATGGTTCT GCCGTGCAGT CCACGGGGCG CGCACAGCAT
    CTCCAGAAAC TGTACAAGTG TGCTTCTTGC CTGAAGGAAT TTCGTTCAAA ACAGGATTTA
    GTGAAACTTG ACATCAATGG TCTGCCATAT GGTCTGTGTG CTAGCTGTGT TAATCTCAGT
    AAAAGTGGTA GTCCAAGTGT CAACATCCCA TCAAGCAGTA ACAGACAAGG CATGGGCCAA
    AATGAGAACT TGAGTTCCAT TGAGAACAAA AGCAAGGCAG GGGGACTGAA GACTCGATGT
    TCTAGTTGCA ATGTTAAATT TGAATCAGAA AGTGAACTCC AAAACCATAT TCAGTCAATC
    CACAGAGAGC TTGTTCCAGA CAGCAACAGT ACACAGCTGA AAACACCACA AGTATCTCCA
    ATGCCCAGAA TCAGCCCCTC CCAAACTGAA GAGAAGAAGA CCTACCAGTG CATCAAATGT
    CAGATGGTTT TCTACAATGA ATGGGATATC CAAGTTCATG TTGCAAACCA CATGATTGAT
    GAAGGACTGA ACCATGAATG CAAGCTCTGC AATCAGACAT TTGACTCTCC TGCCAAACTT
    CAGTGCCACC TGATAGAGCA CAGCTTTGAA GGCATGGGGG GCACCTTCAA ATGTCCAGTG
    TGCTTTACAG TGTTTGTCCA AGCAAACAAG TTGCAGCAGC ACATTTTCTC AGCCCATGGA
    CAAGAGGACA AGATCTATGA CTGCACACAG TGTCCACAGA AGTTCTTCTT CCAAACAGAG
    CTGCAGAATC ATACGATGAC CCAGCATAGC AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32516
    Clone ID Related Accession (Same CDS sequence) XM_004939803.2
    Accession Version XM_004939803.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4221bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product zinc finger protein 521 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004939803.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGATTTCCT CAGTGATTAC GTACAGAGCG AGTCCCTGCG GGTTAGGGGC CCCCTCTGGA 
    GCCATCCTGA TGGCTTTGGG GGCCTTGCTT CCATTTTCCA TTATTATGTG GACTACCGGA
    GCGACAGCGC AGTCCAAGAC CTTGCAGCTG TTTGCTTTTA GGATGTCTCG CCGCAAGCAA
    GCTAAACCGA GATCACTCAA AGAAACCCAG ATTCTAGAAA CAACCAATTG TATGAAGCCT
    TGGGGTTGGC GGAGGAGGAA AAGAGTAGAA GAAAATGAAA CAGAGGACCT GCAGGCAGGT
    GTGGGACACA CAGCCGCGCA GACTGACCCT AACTGTAAAC TAGAAGACAA GACAGAAGAT
    GGAGAAGTGT TAGACTGTAA GAAAAGGCCG GATGAAGGGG AGGAGTTGGA AGAAGAAGCC
    GTGCACAGCT GTGACAGCTG CCTCCAGGTG TTTGAGTCGC TGAGTGATAT CACAGAACAC
    AAGATTAATC AATGTCAACT GACAGATGGA GTGGATGTTG AGGATGACCC CACTTGCTCA
    TGGCCAGCAT CATCGCCTTC CAGCAAGGAC CAGACTTCCC CAAGCCATGG AGAAGGTTGT
    GATTTTGGTG AGGAGGAAGG AGGCCCAGGG TTGCCCTATC AGTGCCAGTT TTGTGACAAA
    TCGTTCAGCC GCCTCAGCTA CTTAAAGCAC CACGAGCAAA GTCACAGTGA CAAACTCCCT
    TTCAAATGCA CTTATTGCAG TCGTCTCTTC AAACACAAAC GAAGCAGGGA CCGCCACATA
    AAGCTTCATA CGGGAGACAA GAAGTATCAC TGCAGTGAGT GTGATGCAGC ATTCTCAAGG
    AGCGATCATC TTAAAATTCA CTTAAAGACT CATACGTCCA ACAAGCCATA TAAATGTGCC
    ATTTGTAGAC GTGGATTCCT GTCATCCAGT TCGCTGCACG GCCACATGCA GGTTCACGAG
    AGGAACAAGG ATGGCTCTCA GTCTGCTTCC CGGATGGAGG ACTGGAAAAT GAAGGACACT
    CAGAAGTGCA GTCAGTGTGA AGAAGGCTTT GATTTTCCTG AAGATCTTCA GAAGCACATT
    GCGGAATGTC ACCCTGAGTG TTCTCCTAAT GAAGACCGAT CTGCTCTCCA GTGTGTTTAC
    TGTCACGAAC TCTTTGTAGA GGAAACGTCT CTGGTGAATC ACATGGAGCA GGCTCATAAT
    GGTGAGAAGA AGAATTCATG CAGCATTTGC TCTGAGAACT TTCACACTGT GGAAGAGCTG
    TACAGCCACA TGGACAGTCA CCAGCAGCCA GAATCATGCA ACCACAGCAA CAGCCCATCA
    TTGGTAACTG TTGGCTACAC CTCAGTCTCA AGTACCACTC CAGATTCCAA TCTCTCTGTG
    GATAGTTCAA CAATGGTGGA AACGGCTCCC CCTATACCAA AAGGCCGTGG AAGAAAGCGG
    GCAGCTCAGC AAGTGCCAGA TATCACTGGT CCTTCAAGTA AACAGGCAAA AGTTACCTAT
    AGCTGCATTT ATTGCAACAA ACAGTTATTT TCCAGCCTTG CAGTTTTGCA GATACACCTG
    AAAACTATGC ATTTAGATAA ACCTGAACAA GCCCATATCT GTCAGTATTG TTTGGAGGTA
    TTGCCATCTC TCTACAATCT GAACGAACAT CTTAAGCAAG TCCACGAAGC TCCGGATCCA
    GGGCTGATTG TTTCCACTAT GCCTGCCATG GTGTACCAGT GCAACTTCTG CTCTGAAGTG
    TTCAATGACC TCAACACGCT TCAAGAACAT ATCCGATGTT CTCATGGATT TGCCAACCCT
    GCTGCTAAGG ACAGTAATGC GTTCTTTTGC CCCCATTGCT ACATGGGGTT TCTTACAGAT
    TCTTCTCTGG AAGAGCATAT TAGACAAGTC CATTGTGATC TTAGCAGTTC CCGTTTTGGT
    TCCCCTGTGC TCGGAACCCC AAAAGATCCA GTAGTGGAAG TGTATTCTTG TTCTTACTGT
    ACAAATTCTC CAATATTTAA TAGCGTTCTT AAACTGAACA AGCATATCAA GGAGAACCAT
    AAGAACATTC CTTTGGCACT GAATTACATC CACAATGGAA AAAAATCCAG AGCCATGAGT
    CCCTTATCTC CTGTAACGAT TGAGCAGACC TCTTTAAAAA TGATGCAGGC GGTTGGAGGT
    GCTCCTCCTC GTCCTGCAGG TGAATATATT TGTAATCAAT GTGGTGCTAA GTATACTTCT
    TTGGACGGTT TTCAGACTCA TTTAAAAACA CATCTTGATA CTGTCCTGCC AAAACTGACC
    TGCCCTCAGT GCAACAAGGA GTTTCCAAAT CAGGAGTCTC TGCTGAAGCA TGTTACCATT
    CATTTTATGA TCACTTCAAC CTACTACATC TGTGAAAGCT GTGACAAGCA GTTTACTTCT
    GTGGATGACT TGCAGAAGCA CCTACTAGAC ATGCATACAT TTGTATTCTT CCGCTGCACC
    TTGTGCCAAG AGGTTTTTGA CTCCAAAGTC TCCATCCAAC TACACTTGGC TGTGAAGCAC
    AGCAATGAAA AGAAGGTATA TAGGTGTACG TCTTGCAACT GGGATTTCCG CAATGAGACT
    GACCTACAGC TTCACGTTAA ACACAACCAC TTGGAGAACC AAGGCAAAGT GCACAAGTGC
    ATCTTCTGTG GTGAGTCCTT TGGTACAGAG GTGGAGCTGC AGTGCCATAT TACTACGCAC
    AGCAAGAAGT ACAACTGTAA GTTCTGCAGC AAAGCTTTCC ATGCCATCAT CCTGCTGGAA
    AAGCATTTAA GGGAAAAACA TTGTGTTTTT GAAACAAAAA CTCCAAATTG TGGAACGAAT
    GGGGCATCTG AACAGGTTCA GAAGGAAGAA GTGGAGCTCC AGACCTTGTT GACAAATAGC
    CAGGAGTCCC ATAACAGCCA TGATGGCAGC GAAGAGGATG TGGACACATC TGAGCCTATG
    TATGGCTGTG ACATTTGTGG AGCGGCTTAC ACCATGGAGA CTCTCCTGCA AAACCACCAG
    CTTCGAGACC ACAACATTCG ACCTGGAGAA AGTGCCATAG TGAAGAAAAA GGCTGAACTC
    ATTAAAGGGA ATTACAAGTG TAATGTGTGT TCTCGAACAT TCTTCTCTGA AAATGGGCTC
    CGAGAACACA TGCAGACACA CTTGGGACCA GTGAAACATT ACATGTGCCC AATCTGTGGC
    GAGCGGTTTC CATCTCTTCT GACTCTTACT GAACATAAAG TCACACATAG TAAGAGCCTG
    GACACCGGAA ACTGCAGGAT TTGCAAGATG CCTCTCCAGA GTGAGGAGGA ATTTTTAGAG
    CATTGCCAGA TGCACCCTGA CTTGAGGAAC TCTTTGACAG GGTTCCGCTG TGTGGTGTGC
    ATGCAGACTG TGACCTCCAC GTTGGAACTG AAAATCCACG GCACATTCCA CATGCAGAAA
    ACTGGAAATG GTTCTGCCGT GCAGTCCACG GGGCGCGCAC AGCATCTCCA GAAACTGTAC
    AAGTGTGCTT CTTGCCTGAA GGAATTTCGT TCAAAACAGG ATTTAGTGAA ACTTGACATC
    AATGGTCTGC CATATGGTCT GTGTGCTAGC TGTGTTAATC TCAGTAAAAG TGGTAGTCCA
    AGTGTCAACA TCCCATCAAG CAGTAACAGA CAAGGCATGG GCCAAAATGA GAACTTGAGT
    TCCATTGAGA ACAAAAGCAA GGCAGGGGGA CTGAAGACTC GATGTTCTAG TTGCAATGTT
    AAATTTGAAT CAGAAAGTGA ACTCCAAAAC CATATTCAGT CAATCCACAG AGAGCTTGTT
    CCAGACAGCA ACAGTACACA GCTGAAAACA CCACAAGTAT CTCCAATGCC CAGAATCAGC
    CCCTCCCAAA CTGAAGAGAA GAAGACCTAC CAGTGCATCA AATGTCAGAT GGTTTTCTAC
    AATGAATGGG ATATCCAAGT TCATGTTGCA AACCACATGA TTGATGAAGG ACTGAACCAT
    GAATGCAAGC TCTGCAATCA GACATTTGAC TCTCCTGCCA AACTTCAGTG CCACCTGATA
    GAGCACAGCT TTGAAGGCAT GGGGGGCACC TTCAAATGTC CAGTGTGCTT TACAGTGTTT
    GTCCAAGCAA ACAAGTTGCA GCAGCACATT TTCTCAGCCC ATGGACAAGA GGACAAGATC
    TATGACTGCA CACAGTGTCC ACAGAAGTTC TTCTTCCAAA CAGAGCTGCA GAATCATACG
    ATGACCCAGC ATAGCAGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004939860.2
    CDS26..4246
    Translation

    Target ORF information:

    RefSeq Version XM_004939803.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004939803.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGATTTCCT CAGTGATTAC GTACAGAGCG AGTCCCTGCG GGTTAGGGGC CCCCTCTGGA 
    GCCATCCTGA TGGCTTTGGG GGCCTTGCTT CCATTTTCCA TTATTATGTG GACTACCGGA
    GCGACAGCGC AGTCCAAGAC CTTGCAGCTG TTTGCTTTTA GGATGTCTCG CCGCAAGCAA
    GCTAAACCGA GATCACTCAA AGAAACCCAG ATTCTAGAAA CAACCAATTG TATGAAGCCT
    TGGGGTTGGC GGAGGAGGAA AAGAGTAGAA GAAAATGAAA CAGAGGACCT GCAGGCAGGT
    GTGGGACACA CAGCCGCGCA GACTGACCCT AACTGTAAAC TAGAAGACAA GACAGAAGAT
    GGAGAAGTGT TAGACTGTAA GAAAAGGCCG GATGAAGGGG AGGAGTTGGA AGAAGAAGCC
    GTGCACAGCT GTGACAGCTG CCTCCAGGTG TTTGAGTCGC TGAGTGATAT CACAGAACAC
    AAGATTAATC AATGTCAACT GACAGATGGA GTGGATGTTG AGGATGACCC CACTTGCTCA
    TGGCCAGCAT CATCGCCTTC CAGCAAGGAC CAGACTTCCC CAAGCCATGG AGAAGGTTGT
    GATTTTGGTG AGGAGGAAGG AGGCCCAGGG TTGCCCTATC AGTGCCAGTT TTGTGACAAA
    TCGTTCAGCC GCCTCAGCTA CTTAAAGCAC CACGAGCAAA GTCACAGTGA CAAACTCCCT
    TTCAAATGCA CTTATTGCAG TCGTCTCTTC AAACACAAAC GAAGCAGGGA CCGCCACATA
    AAGCTTCATA CGGGAGACAA GAAGTATCAC TGCAGTGAGT GTGATGCAGC ATTCTCAAGG
    AGCGATCATC TTAAAATTCA CTTAAAGACT CATACGTCCA ACAAGCCATA TAAATGTGCC
    ATTTGTAGAC GTGGATTCCT GTCATCCAGT TCGCTGCACG GCCACATGCA GGTTCACGAG
    AGGAACAAGG ATGGCTCTCA GTCTGCTTCC CGGATGGAGG ACTGGAAAAT GAAGGACACT
    CAGAAGTGCA GTCAGTGTGA AGAAGGCTTT GATTTTCCTG AAGATCTTCA GAAGCACATT
    GCGGAATGTC ACCCTGAGTG TTCTCCTAAT GAAGACCGAT CTGCTCTCCA GTGTGTTTAC
    TGTCACGAAC TCTTTGTAGA GGAAACGTCT CTGGTGAATC ACATGGAGCA GGCTCATAAT
    GGTGAGAAGA AGAATTCATG CAGCATTTGC TCTGAGAACT TTCACACTGT GGAAGAGCTG
    TACAGCCACA TGGACAGTCA CCAGCAGCCA GAATCATGCA ACCACAGCAA CAGCCCATCA
    TTGGTAACTG TTGGCTACAC CTCAGTCTCA AGTACCACTC CAGATTCCAA TCTCTCTGTG
    GATAGTTCAA CAATGGTGGA AACGGCTCCC CCTATACCAA AAGGCCGTGG AAGAAAGCGG
    GCAGCTCAGC AAGTGCCAGA TATCACTGGT CCTTCAAGTA AACAGGCAAA AGTTACCTAT
    AGCTGCATTT ATTGCAACAA ACAGTTATTT TCCAGCCTTG CAGTTTTGCA GATACACCTG
    AAAACTATGC ATTTAGATAA ACCTGAACAA GCCCATATCT GTCAGTATTG TTTGGAGGTA
    TTGCCATCTC TCTACAATCT GAACGAACAT CTTAAGCAAG TCCACGAAGC TCCGGATCCA
    GGGCTGATTG TTTCCACTAT GCCTGCCATG GTGTACCAGT GCAACTTCTG CTCTGAAGTG
    TTCAATGACC TCAACACGCT TCAAGAACAT ATCCGATGTT CTCATGGATT TGCCAACCCT
    GCTGCTAAGG ACAGTAATGC GTTCTTTTGC CCCCATTGCT ACATGGGGTT TCTTACAGAT
    TCTTCTCTGG AAGAGCATAT TAGACAAGTC CATTGTGATC TTAGCAGTTC CCGTTTTGGT
    TCCCCTGTGC TCGGAACCCC AAAAGATCCA GTAGTGGAAG TGTATTCTTG TTCTTACTGT
    ACAAATTCTC CAATATTTAA TAGCGTTCTT AAACTGAACA AGCATATCAA GGAGAACCAT
    AAGAACATTC CTTTGGCACT GAATTACATC CACAATGGAA AAAAATCCAG AGCCATGAGT
    CCCTTATCTC CTGTAACGAT TGAGCAGACC TCTTTAAAAA TGATGCAGGC GGTTGGAGGT
    GCTCCTCCTC GTCCTGCAGG TGAATATATT TGTAATCAAT GTGGTGCTAA GTATACTTCT
    TTGGACGGTT TTCAGACTCA TTTAAAAACA CATCTTGATA CTGTCCTGCC AAAACTGACC
    TGCCCTCAGT GCAACAAGGA GTTTCCAAAT CAGGAGTCTC TGCTGAAGCA TGTTACCATT
    CATTTTATGA TCACTTCAAC CTACTACATC TGTGAAAGCT GTGACAAGCA GTTTACTTCT
    GTGGATGACT TGCAGAAGCA CCTACTAGAC ATGCATACAT TTGTATTCTT CCGCTGCACC
    TTGTGCCAAG AGGTTTTTGA CTCCAAAGTC TCCATCCAAC TACACTTGGC TGTGAAGCAC
    AGCAATGAAA AGAAGGTATA TAGGTGTACG TCTTGCAACT GGGATTTCCG CAATGAGACT
    GACCTACAGC TTCACGTTAA ACACAACCAC TTGGAGAACC AAGGCAAAGT GCACAAGTGC
    ATCTTCTGTG GTGAGTCCTT TGGTACAGAG GTGGAGCTGC AGTGCCATAT TACTACGCAC
    AGCAAGAAGT ACAACTGTAA GTTCTGCAGC AAAGCTTTCC ATGCCATCAT CCTGCTGGAA
    AAGCATTTAA GGGAAAAACA TTGTGTTTTT GAAACAAAAA CTCCAAATTG TGGAACGAAT
    GGGGCATCTG AACAGGTTCA GAAGGAAGAA GTGGAGCTCC AGACCTTGTT GACAAATAGC
    CAGGAGTCCC ATAACAGCCA TGATGGCAGC GAAGAGGATG TGGACACATC TGAGCCTATG
    TATGGCTGTG ACATTTGTGG AGCGGCTTAC ACCATGGAGA CTCTCCTGCA AAACCACCAG
    CTTCGAGACC ACAACATTCG ACCTGGAGAA AGTGCCATAG TGAAGAAAAA GGCTGAACTC
    ATTAAAGGGA ATTACAAGTG TAATGTGTGT TCTCGAACAT TCTTCTCTGA AAATGGGCTC
    CGAGAACACA TGCAGACACA CTTGGGACCA GTGAAACATT ACATGTGCCC AATCTGTGGC
    GAGCGGTTTC CATCTCTTCT GACTCTTACT GAACATAAAG TCACACATAG TAAGAGCCTG
    GACACCGGAA ACTGCAGGAT TTGCAAGATG CCTCTCCAGA GTGAGGAGGA ATTTTTAGAG
    CATTGCCAGA TGCACCCTGA CTTGAGGAAC TCTTTGACAG GGTTCCGCTG TGTGGTGTGC
    ATGCAGACTG TGACCTCCAC GTTGGAACTG AAAATCCACG GCACATTCCA CATGCAGAAA
    ACTGGAAATG GTTCTGCCGT GCAGTCCACG GGGCGCGCAC AGCATCTCCA GAAACTGTAC
    AAGTGTGCTT CTTGCCTGAA GGAATTTCGT TCAAAACAGG ATTTAGTGAA ACTTGACATC
    AATGGTCTGC CATATGGTCT GTGTGCTAGC TGTGTTAATC TCAGTAAAAG TGGTAGTCCA
    AGTGTCAACA TCCCATCAAG CAGTAACAGA CAAGGCATGG GCCAAAATGA GAACTTGAGT
    TCCATTGAGA ACAAAAGCAA GGCAGGGGGA CTGAAGACTC GATGTTCTAG TTGCAATGTT
    AAATTTGAAT CAGAAAGTGA ACTCCAAAAC CATATTCAGT CAATCCACAG AGAGCTTGTT
    CCAGACAGCA ACAGTACACA GCTGAAAACA CCACAAGTAT CTCCAATGCC CAGAATCAGC
    CCCTCCCAAA CTGAAGAGAA GAAGACCTAC CAGTGCATCA AATGTCAGAT GGTTTTCTAC
    AATGAATGGG ATATCCAAGT TCATGTTGCA AACCACATGA TTGATGAAGG ACTGAACCAT
    GAATGCAAGC TCTGCAATCA GACATTTGAC TCTCCTGCCA AACTTCAGTG CCACCTGATA
    GAGCACAGCT TTGAAGGCAT GGGGGGCACC TTCAAATGTC CAGTGTGCTT TACAGTGTTT
    GTCCAAGCAA ACAAGTTGCA GCAGCACATT TTCTCAGCCC ATGGACAAGA GGACAAGATC
    TATGACTGCA CACAGTGTCC ACAGAAGTTC TTCTTCCAAA CAGAGCTGCA GAATCATACG
    ATGACCCAGC ATAGCAGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32517
    Clone ID Related Accession (Same CDS sequence) XM_015277992.1 , XM_015277992.2
    Accession Version XM_015277992.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product zinc finger protein 521 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCAGGTTC ACGAGAGGAA CAAGGATGGC TCTCAGTCTG CTTCCCGGAT GGAGGACTGG 
    AAAATGAAGG ACACTCAGAA GTGCAGTCAG TGTGAAGAAG GCTTTGATTT TCCTGAAGAT
    CTTCAGAAGC ACATTGCGGA ATGTCACCCT GAGTGTTCTC CTAATGAAGA CCGATCTGCT
    CTCCAGTGTG TTTACTGTCA CGAACTCTTT GTAGAGGAAA CGTCTCTGGT GAATCACATG
    GAGCAGGCTC ATAATGGTGA GAAGAAGAAT TCATGCAGCA TTTGCTCTGA GAACTTTCAC
    ACTGTGGAAG AGCTGTACAG CCACATGGAC AGTCACCAGC AGCCAGAATC ATGCAACCAC
    AGCAACAGCC CATCATTGGT AACTGTTGGC TACACCTCAG TCTCAAGTAC CACTCCAGAT
    TCCAATCTCT CTGTGGATAG TTCAACAATG GTGGAAACGG CTCCCCCTAT ACCAAAAGGC
    CGTGGAAGAA AGCGGGCAGC TCAGCAAGTG CCAGATATCA CTGGTCCTTC AAGTAAACAG
    GCAAAAGTTA CCTATAGCTG CATTTATTGC AACAAACAGT TATTTTCCAG CCTTGCAGTT
    TTGCAGATAC ACCTGAAAAC TATGCATTTA GATAAACCTG AACAAGCCCA TATCTGTCAG
    TATTGTTTGG AGGTATTGCC ATCTCTCTAC AATCTGAACG AACATCTTAA GCAAGTCCAC
    GAAGCTCCGG ATCCAGGGCT GATTGTTTCC ACTATGCCTG CCATGGTGTA CCAGTGCAAC
    TTCTGCTCTG AAGTGTTCAA TGACCTCAAC ACGCTTCAAG AACATATCCG ATGTTCTCAT
    GGATTTGCCA ACCCTGCTGC TAAGGACAGT AATGCGTTCT TTTGCCCCCA TTGCTACATG
    GGGTTTCTTA CAGATTCTTC TCTGGAAGAG CATATTAGAC AAGTCCATTG TGATCTTAGC
    AGTTCCCGTT TTGGTTCCCC TGTGCTCGGA ACCCCAAAAG ATCCAGTAGT GGAAGTGTAT
    TCTTGTTCTT ACTGTACAAA TTCTCCAATA TTTAATAGCG TTCTTAAACT GAACAAGCAT
    ATCAAGGAGA ACCATAAGAA CATTCCTTTG GCACTGAATT ACATCCACAA TGGAAAAAAA
    TCCAGAGCCA TGAGTCCCTT ATCTCCTGTA ACGATTGAGC AGACCTCTTT AAAAATGATG
    CAGGCGGTTG GAGGTGCTCC TCCTCGTCCT GCAGGTGAAT ATATTTGTAA TCAATGTGGT
    GCTAAGTATA CTTCTTTGGA CGGTTTTCAG ACTCATTTAA AAACACATCT TGATACTGTC
    CTGCCAAAAC TGACCTGCCC TCAGTGCAAC AAGGAGTTTC CAAATCAGGA GTCTCTGCTG
    AAGCATGTTA CCATTCATTT TATGATCACT TCAACCTACT ACATCTGTGA AAGCTGTGAC
    AAGCAGTTTA CTTCTGTGGA TGACTTGCAG AAGCACCTAC TAGACATGCA TACATTTGTA
    TTCTTCCGCT GCACCTTGTG CCAAGAGGTT TTTGACTCCA AAGTCTCCAT CCAACTACAC
    TTGGCTGTGA AGCACAGCAA TGAAAAGAAG GTATATAGGT GTACGTCTTG CAACTGGGAT
    TTCCGCAATG AGACTGACCT ACAGCTTCAC GTTAAACACA ACCACTTGGA GAACCAAGGC
    AAAGTGCACA AGTGCATCTT CTGTGGTGAG TCCTTTGGTA CAGAGGTGGA GCTGCAGTGC
    CATATTACTA CGCACAGCAA GAAGTACAAC TGTAAGTTCT GCAGCAAAGC TTTCCATGCC
    ATCATCCTGC TGGAAAAGCA TTTAAGGGAA AAACATTGTG TTTTTGAAAC AAAAACTCCA
    AATTGTGGAA CGAATGGGGC ATCTGAACAG GTTCAGAAGG AAGAAGTGGA GCTCCAGACC
    TTGTTGACAA ATAGCCAGGA GTCCCATAAC AGCCATGATG GCAGCGAAGA GGATGTGGAC
    ACATCTGAGC CTATGTATGG CTGTGACATT TGTGGAGCGG CTTACACCAT GGAGACTCTC
    CTGCAAAACC ACCAGCTTCG AGACCACAAC ATTCGACCTG GAGAAAGTGC CATAGTGAAG
    AAAAAGGCTG AACTCATTAA AGGGAATTAC AAGTGTAATG TGTGTTCTCG AACATTCTTC
    TCTGAAAATG GGCTCCGAGA ACACATGCAG ACACACTTGG GACCAGTGAA ACATTACATG
    TGCCCAATCT GTGGCGAGCG GTTTCCATCT CTTCTGACTC TTACTGAACA TAAAGTCACA
    CATAGTAAGA GCCTGGACAC CGGAAACTGC AGGATTTGCA AGATGCCTCT CCAGAGTGAG
    GAGGAATTTT TAGAGCATTG CCAGATGCAC CCTGACTTGA GGAACTCTTT GACAGGGTTC
    CGCTGTGTGG TGTGCATGCA GACTGTGACC TCCACGTTGG AACTGAAAAT CCACGGCACA
    TTCCACATGC AGAAAACTGG AAATGGTTCT GCCGTGCAGT CCACGGGGCG CGCACAGCAT
    CTCCAGAAAC TGTACAAGTG TGCTTCTTGC CTGAAGGAAT TTCGTTCAAA ACAGGATTTA
    GTGAAACTTG ACATCAATGG TCTGCCATAT GGTCTGTGTG CTAGCTGTGT TAATCTCAGT
    AAAAGTGGTA GTCCAAGTGT CAACATCCCA TCAAGCAGTA ACAGACAAGG CATGGGCCAA
    AATGAGAACT TGAGTTCCAT TGAGAACAAA AGCAAGGCAG GGGGACTGAA GACTCGATGT
    TCTAGTTGCA ATGTTAAATT TGAATCAGAA AGTGAACTCC AAAACCATAT TCAGTCAATC
    CACAGAGAGC TTGTTCCAGA CAGCAACAGT ACACAGCTGA AAACACCACA AGTATCTCCA
    ATGCCCAGAA TCAGCCCCTC CCAAACTGAA GAGAAGAAGA CCTACCAGTG CATCAAATGT
    CAGATGGTTT TCTACAATGA ATGGGATATC CAAGTTCATG TTGCAAACCA CATGATTGAT
    GAAGGACTGA ACCATGAATG CAAGCTCTGC AATCAGACAT TTGACTCTCC TGCCAAACTT
    CAGTGCCACC TGATAGAGCA CAGCTTTGAA GGCATGGGGG GCACCTTCAA ATGTCCAGTG
    TGCTTTACAG TGTTTGTCCA AGCAAACAAG TTGCAGCAGC ACATTTTCTC AGCCCATGGA
    CAAGAGGACA AGATCTATGA CTGCACACAG TGTCCACAGA AGTTCTTCTT CCAAACAGAG
    CTGCAGAATC ATACGATGAC CCAGCATAGC AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133478.1
    CDS870..4145
    Translation

    Target ORF information:

    RefSeq Version XM_015277992.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277992.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCAGGTTC ACGAGAGGAA CAAGGATGGC TCTCAGTCTG CTTCCCGGAT GGAGGACTGG 
    AAAATGAAGG ACACTCAGAA GTGCAGTCAG TGTGAAGAAG GCTTTGATTT TCCTGAAGAT
    CTTCAGAAGC ACATTGCGGA ATGTCACCCT GAGTGTTCTC CTAATGAAGA CCGATCTGCT
    CTCCAGTGTG TTTACTGTCA CGAACTCTTT GTAGAGGAAA CGTCTCTGGT GAATCACATG
    GAGCAGGCTC ATAATGGTGA GAAGAAGAAT TCATGCAGCA TTTGCTCTGA GAACTTTCAC
    ACTGTGGAAG AGCTGTACAG CCACATGGAC AGTCACCAGC AGCCAGAATC ATGCAACCAC
    AGCAACAGCC CATCATTGGT AACTGTTGGC TACACCTCAG TCTCAAGTAC CACTCCAGAT
    TCCAATCTCT CTGTGGATAG TTCAACAATG GTGGAAACGG CTCCCCCTAT ACCAAAAGGC
    CGTGGAAGAA AGCGGGCAGC TCAGCAAGTG CCAGATATCA CTGGTCCTTC AAGTAAACAG
    GCAAAAGTTA CCTATAGCTG CATTTATTGC AACAAACAGT TATTTTCCAG CCTTGCAGTT
    TTGCAGATAC ACCTGAAAAC TATGCATTTA GATAAACCTG AACAAGCCCA TATCTGTCAG
    TATTGTTTGG AGGTATTGCC ATCTCTCTAC AATCTGAACG AACATCTTAA GCAAGTCCAC
    GAAGCTCCGG ATCCAGGGCT GATTGTTTCC ACTATGCCTG CCATGGTGTA CCAGTGCAAC
    TTCTGCTCTG AAGTGTTCAA TGACCTCAAC ACGCTTCAAG AACATATCCG ATGTTCTCAT
    GGATTTGCCA ACCCTGCTGC TAAGGACAGT AATGCGTTCT TTTGCCCCCA TTGCTACATG
    GGGTTTCTTA CAGATTCTTC TCTGGAAGAG CATATTAGAC AAGTCCATTG TGATCTTAGC
    AGTTCCCGTT TTGGTTCCCC TGTGCTCGGA ACCCCAAAAG ATCCAGTAGT GGAAGTGTAT
    TCTTGTTCTT ACTGTACAAA TTCTCCAATA TTTAATAGCG TTCTTAAACT GAACAAGCAT
    ATCAAGGAGA ACCATAAGAA CATTCCTTTG GCACTGAATT ACATCCACAA TGGAAAAAAA
    TCCAGAGCCA TGAGTCCCTT ATCTCCTGTA ACGATTGAGC AGACCTCTTT AAAAATGATG
    CAGGCGGTTG GAGGTGCTCC TCCTCGTCCT GCAGGTGAAT ATATTTGTAA TCAATGTGGT
    GCTAAGTATA CTTCTTTGGA CGGTTTTCAG ACTCATTTAA AAACACATCT TGATACTGTC
    CTGCCAAAAC TGACCTGCCC TCAGTGCAAC AAGGAGTTTC CAAATCAGGA GTCTCTGCTG
    AAGCATGTTA CCATTCATTT TATGATCACT TCAACCTACT ACATCTGTGA AAGCTGTGAC
    AAGCAGTTTA CTTCTGTGGA TGACTTGCAG AAGCACCTAC TAGACATGCA TACATTTGTA
    TTCTTCCGCT GCACCTTGTG CCAAGAGGTT TTTGACTCCA AAGTCTCCAT CCAACTACAC
    TTGGCTGTGA AGCACAGCAA TGAAAAGAAG GTATATAGGT GTACGTCTTG CAACTGGGAT
    TTCCGCAATG AGACTGACCT ACAGCTTCAC GTTAAACACA ACCACTTGGA GAACCAAGGC
    AAAGTGCACA AGTGCATCTT CTGTGGTGAG TCCTTTGGTA CAGAGGTGGA GCTGCAGTGC
    CATATTACTA CGCACAGCAA GAAGTACAAC TGTAAGTTCT GCAGCAAAGC TTTCCATGCC
    ATCATCCTGC TGGAAAAGCA TTTAAGGGAA AAACATTGTG TTTTTGAAAC AAAAACTCCA
    AATTGTGGAA CGAATGGGGC ATCTGAACAG GTTCAGAAGG AAGAAGTGGA GCTCCAGACC
    TTGTTGACAA ATAGCCAGGA GTCCCATAAC AGCCATGATG GCAGCGAAGA GGATGTGGAC
    ACATCTGAGC CTATGTATGG CTGTGACATT TGTGGAGCGG CTTACACCAT GGAGACTCTC
    CTGCAAAACC ACCAGCTTCG AGACCACAAC ATTCGACCTG GAGAAAGTGC CATAGTGAAG
    AAAAAGGCTG AACTCATTAA AGGGAATTAC AAGTGTAATG TGTGTTCTCG AACATTCTTC
    TCTGAAAATG GGCTCCGAGA ACACATGCAG ACACACTTGG GACCAGTGAA ACATTACATG
    TGCCCAATCT GTGGCGAGCG GTTTCCATCT CTTCTGACTC TTACTGAACA TAAAGTCACA
    CATAGTAAGA GCCTGGACAC CGGAAACTGC AGGATTTGCA AGATGCCTCT CCAGAGTGAG
    GAGGAATTTT TAGAGCATTG CCAGATGCAC CCTGACTTGA GGAACTCTTT GACAGGGTTC
    CGCTGTGTGG TGTGCATGCA GACTGTGACC TCCACGTTGG AACTGAAAAT CCACGGCACA
    TTCCACATGC AGAAAACTGG AAATGGTTCT GCCGTGCAGT CCACGGGGCG CGCACAGCAT
    CTCCAGAAAC TGTACAAGTG TGCTTCTTGC CTGAAGGAAT TTCGTTCAAA ACAGGATTTA
    GTGAAACTTG ACATCAATGG TCTGCCATAT GGTCTGTGTG CTAGCTGTGT TAATCTCAGT
    AAAAGTGGTA GTCCAAGTGT CAACATCCCA TCAAGCAGTA ACAGACAAGG CATGGGCCAA
    AATGAGAACT TGAGTTCCAT TGAGAACAAA AGCAAGGCAG GGGGACTGAA GACTCGATGT
    TCTAGTTGCA ATGTTAAATT TGAATCAGAA AGTGAACTCC AAAACCATAT TCAGTCAATC
    CACAGAGAGC TTGTTCCAGA CAGCAACAGT ACACAGCTGA AAACACCACA AGTATCTCCA
    ATGCCCAGAA TCAGCCCCTC CCAAACTGAA GAGAAGAAGA CCTACCAGTG CATCAAATGT
    CAGATGGTTT TCTACAATGA ATGGGATATC CAAGTTCATG TTGCAAACCA CATGATTGAT
    GAAGGACTGA ACCATGAATG CAAGCTCTGC AATCAGACAT TTGACTCTCC TGCCAAACTT
    CAGTGCCACC TGATAGAGCA CAGCTTTGAA GGCATGGGGG GCACCTTCAA ATGTCCAGTG
    TGCTTTACAG TGTTTGTCCA AGCAAACAAG TTGCAGCAGC ACATTTTCTC AGCCCATGGA
    CAAGAGGACA AGATCTATGA CTGCACACAG TGTCCACAGA AGTTCTTCTT CCAAACAGAG
    CTGCAGAATC ATACGATGAC CCAGCATAGC AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32517
    Clone ID Related Accession (Same CDS sequence) XM_015277992.1 , XM_015277992.2
    Accession Version XM_015277992.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product zinc finger protein 521 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277992.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCAGGTTC ACGAGAGGAA CAAGGATGGC TCTCAGTCTG CTTCCCGGAT GGAGGACTGG 
    AAAATGAAGG ACACTCAGAA GTGCAGTCAG TGTGAAGAAG GCTTTGATTT TCCTGAAGAT
    CTTCAGAAGC ACATTGCGGA ATGTCACCCT GAGTGTTCTC CTAATGAAGA CCGATCTGCT
    CTCCAGTGTG TTTACTGTCA CGAACTCTTT GTAGAGGAAA CGTCTCTGGT GAATCACATG
    GAGCAGGCTC ATAATGGTGA GAAGAAGAAT TCATGCAGCA TTTGCTCTGA GAACTTTCAC
    ACTGTGGAAG AGCTGTACAG CCACATGGAC AGTCACCAGC AGCCAGAATC ATGCAACCAC
    AGCAACAGCC CATCATTGGT AACTGTTGGC TACACCTCAG TCTCAAGTAC CACTCCAGAT
    TCCAATCTCT CTGTGGATAG TTCAACAATG GTGGAAACGG CTCCCCCTAT ACCAAAAGGC
    CGTGGAAGAA AGCGGGCAGC TCAGCAAGTG CCAGATATCA CTGGTCCTTC AAGTAAACAG
    GCAAAAGTTA CCTATAGCTG CATTTATTGC AACAAACAGT TATTTTCCAG CCTTGCAGTT
    TTGCAGATAC ACCTGAAAAC TATGCATTTA GATAAACCTG AACAAGCCCA TATCTGTCAG
    TATTGTTTGG AGGTATTGCC ATCTCTCTAC AATCTGAACG AACATCTTAA GCAAGTCCAC
    GAAGCTCCGG ATCCAGGGCT GATTGTTTCC ACTATGCCTG CCATGGTGTA CCAGTGCAAC
    TTCTGCTCTG AAGTGTTCAA TGACCTCAAC ACGCTTCAAG AACATATCCG ATGTTCTCAT
    GGATTTGCCA ACCCTGCTGC TAAGGACAGT AATGCGTTCT TTTGCCCCCA TTGCTACATG
    GGGTTTCTTA CAGATTCTTC TCTGGAAGAG CATATTAGAC AAGTCCATTG TGATCTTAGC
    AGTTCCCGTT TTGGTTCCCC TGTGCTCGGA ACCCCAAAAG ATCCAGTAGT GGAAGTGTAT
    TCTTGTTCTT ACTGTACAAA TTCTCCAATA TTTAATAGCG TTCTTAAACT GAACAAGCAT
    ATCAAGGAGA ACCATAAGAA CATTCCTTTG GCACTGAATT ACATCCACAA TGGAAAAAAA
    TCCAGAGCCA TGAGTCCCTT ATCTCCTGTA ACGATTGAGC AGACCTCTTT AAAAATGATG
    CAGGCGGTTG GAGGTGCTCC TCCTCGTCCT GCAGGTGAAT ATATTTGTAA TCAATGTGGT
    GCTAAGTATA CTTCTTTGGA CGGTTTTCAG ACTCATTTAA AAACACATCT TGATACTGTC
    CTGCCAAAAC TGACCTGCCC TCAGTGCAAC AAGGAGTTTC CAAATCAGGA GTCTCTGCTG
    AAGCATGTTA CCATTCATTT TATGATCACT TCAACCTACT ACATCTGTGA AAGCTGTGAC
    AAGCAGTTTA CTTCTGTGGA TGACTTGCAG AAGCACCTAC TAGACATGCA TACATTTGTA
    TTCTTCCGCT GCACCTTGTG CCAAGAGGTT TTTGACTCCA AAGTCTCCAT CCAACTACAC
    TTGGCTGTGA AGCACAGCAA TGAAAAGAAG GTATATAGGT GTACGTCTTG CAACTGGGAT
    TTCCGCAATG AGACTGACCT ACAGCTTCAC GTTAAACACA ACCACTTGGA GAACCAAGGC
    AAAGTGCACA AGTGCATCTT CTGTGGTGAG TCCTTTGGTA CAGAGGTGGA GCTGCAGTGC
    CATATTACTA CGCACAGCAA GAAGTACAAC TGTAAGTTCT GCAGCAAAGC TTTCCATGCC
    ATCATCCTGC TGGAAAAGCA TTTAAGGGAA AAACATTGTG TTTTTGAAAC AAAAACTCCA
    AATTGTGGAA CGAATGGGGC ATCTGAACAG GTTCAGAAGG AAGAAGTGGA GCTCCAGACC
    TTGTTGACAA ATAGCCAGGA GTCCCATAAC AGCCATGATG GCAGCGAAGA GGATGTGGAC
    ACATCTGAGC CTATGTATGG CTGTGACATT TGTGGAGCGG CTTACACCAT GGAGACTCTC
    CTGCAAAACC ACCAGCTTCG AGACCACAAC ATTCGACCTG GAGAAAGTGC CATAGTGAAG
    AAAAAGGCTG AACTCATTAA AGGGAATTAC AAGTGTAATG TGTGTTCTCG AACATTCTTC
    TCTGAAAATG GGCTCCGAGA ACACATGCAG ACACACTTGG GACCAGTGAA ACATTACATG
    TGCCCAATCT GTGGCGAGCG GTTTCCATCT CTTCTGACTC TTACTGAACA TAAAGTCACA
    CATAGTAAGA GCCTGGACAC CGGAAACTGC AGGATTTGCA AGATGCCTCT CCAGAGTGAG
    GAGGAATTTT TAGAGCATTG CCAGATGCAC CCTGACTTGA GGAACTCTTT GACAGGGTTC
    CGCTGTGTGG TGTGCATGCA GACTGTGACC TCCACGTTGG AACTGAAAAT CCACGGCACA
    TTCCACATGC AGAAAACTGG AAATGGTTCT GCCGTGCAGT CCACGGGGCG CGCACAGCAT
    CTCCAGAAAC TGTACAAGTG TGCTTCTTGC CTGAAGGAAT TTCGTTCAAA ACAGGATTTA
    GTGAAACTTG ACATCAATGG TCTGCCATAT GGTCTGTGTG CTAGCTGTGT TAATCTCAGT
    AAAAGTGGTA GTCCAAGTGT CAACATCCCA TCAAGCAGTA ACAGACAAGG CATGGGCCAA
    AATGAGAACT TGAGTTCCAT TGAGAACAAA AGCAAGGCAG GGGGACTGAA GACTCGATGT
    TCTAGTTGCA ATGTTAAATT TGAATCAGAA AGTGAACTCC AAAACCATAT TCAGTCAATC
    CACAGAGAGC TTGTTCCAGA CAGCAACAGT ACACAGCTGA AAACACCACA AGTATCTCCA
    ATGCCCAGAA TCAGCCCCTC CCAAACTGAA GAGAAGAAGA CCTACCAGTG CATCAAATGT
    CAGATGGTTT TCTACAATGA ATGGGATATC CAAGTTCATG TTGCAAACCA CATGATTGAT
    GAAGGACTGA ACCATGAATG CAAGCTCTGC AATCAGACAT TTGACTCTCC TGCCAAACTT
    CAGTGCCACC TGATAGAGCA CAGCTTTGAA GGCATGGGGG GCACCTTCAA ATGTCCAGTG
    TGCTTTACAG TGTTTGTCCA AGCAAACAAG TTGCAGCAGC ACATTTTCTC AGCCCATGGA
    CAAGAGGACA AGATCTATGA CTGCACACAG TGTCCACAGA AGTTCTTCTT CCAAACAGAG
    CTGCAGAATC ATACGATGAC CCAGCATAGC AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133478.1
    CDS904..4179
    Translation

    Target ORF information:

    RefSeq Version XM_015277992.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus zinc finger protein 521 (ZNF521), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277992.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCAGGTTC ACGAGAGGAA CAAGGATGGC TCTCAGTCTG CTTCCCGGAT GGAGGACTGG 
    AAAATGAAGG ACACTCAGAA GTGCAGTCAG TGTGAAGAAG GCTTTGATTT TCCTGAAGAT
    CTTCAGAAGC ACATTGCGGA ATGTCACCCT GAGTGTTCTC CTAATGAAGA CCGATCTGCT
    CTCCAGTGTG TTTACTGTCA CGAACTCTTT GTAGAGGAAA CGTCTCTGGT GAATCACATG
    GAGCAGGCTC ATAATGGTGA GAAGAAGAAT TCATGCAGCA TTTGCTCTGA GAACTTTCAC
    ACTGTGGAAG AGCTGTACAG CCACATGGAC AGTCACCAGC AGCCAGAATC ATGCAACCAC
    AGCAACAGCC CATCATTGGT AACTGTTGGC TACACCTCAG TCTCAAGTAC CACTCCAGAT
    TCCAATCTCT CTGTGGATAG TTCAACAATG GTGGAAACGG CTCCCCCTAT ACCAAAAGGC
    CGTGGAAGAA AGCGGGCAGC TCAGCAAGTG CCAGATATCA CTGGTCCTTC AAGTAAACAG
    GCAAAAGTTA CCTATAGCTG CATTTATTGC AACAAACAGT TATTTTCCAG CCTTGCAGTT
    TTGCAGATAC ACCTGAAAAC TATGCATTTA GATAAACCTG AACAAGCCCA TATCTGTCAG
    TATTGTTTGG AGGTATTGCC ATCTCTCTAC AATCTGAACG AACATCTTAA GCAAGTCCAC
    GAAGCTCCGG ATCCAGGGCT GATTGTTTCC ACTATGCCTG CCATGGTGTA CCAGTGCAAC
    TTCTGCTCTG AAGTGTTCAA TGACCTCAAC ACGCTTCAAG AACATATCCG ATGTTCTCAT
    GGATTTGCCA ACCCTGCTGC TAAGGACAGT AATGCGTTCT TTTGCCCCCA TTGCTACATG
    GGGTTTCTTA CAGATTCTTC TCTGGAAGAG CATATTAGAC AAGTCCATTG TGATCTTAGC
    AGTTCCCGTT TTGGTTCCCC TGTGCTCGGA ACCCCAAAAG ATCCAGTAGT GGAAGTGTAT
    TCTTGTTCTT ACTGTACAAA TTCTCCAATA TTTAATAGCG TTCTTAAACT GAACAAGCAT
    ATCAAGGAGA ACCATAAGAA CATTCCTTTG GCACTGAATT ACATCCACAA TGGAAAAAAA
    TCCAGAGCCA TGAGTCCCTT ATCTCCTGTA ACGATTGAGC AGACCTCTTT AAAAATGATG
    CAGGCGGTTG GAGGTGCTCC TCCTCGTCCT GCAGGTGAAT ATATTTGTAA TCAATGTGGT
    GCTAAGTATA CTTCTTTGGA CGGTTTTCAG ACTCATTTAA AAACACATCT TGATACTGTC
    CTGCCAAAAC TGACCTGCCC TCAGTGCAAC AAGGAGTTTC CAAATCAGGA GTCTCTGCTG
    AAGCATGTTA CCATTCATTT TATGATCACT TCAACCTACT ACATCTGTGA AAGCTGTGAC
    AAGCAGTTTA CTTCTGTGGA TGACTTGCAG AAGCACCTAC TAGACATGCA TACATTTGTA
    TTCTTCCGCT GCACCTTGTG CCAAGAGGTT TTTGACTCCA AAGTCTCCAT CCAACTACAC
    TTGGCTGTGA AGCACAGCAA TGAAAAGAAG GTATATAGGT GTACGTCTTG CAACTGGGAT
    TTCCGCAATG AGACTGACCT ACAGCTTCAC GTTAAACACA ACCACTTGGA GAACCAAGGC
    AAAGTGCACA AGTGCATCTT CTGTGGTGAG TCCTTTGGTA CAGAGGTGGA GCTGCAGTGC
    CATATTACTA CGCACAGCAA GAAGTACAAC TGTAAGTTCT GCAGCAAAGC TTTCCATGCC
    ATCATCCTGC TGGAAAAGCA TTTAAGGGAA AAACATTGTG TTTTTGAAAC AAAAACTCCA
    AATTGTGGAA CGAATGGGGC ATCTGAACAG GTTCAGAAGG AAGAAGTGGA GCTCCAGACC
    TTGTTGACAA ATAGCCAGGA GTCCCATAAC AGCCATGATG GCAGCGAAGA GGATGTGGAC
    ACATCTGAGC CTATGTATGG CTGTGACATT TGTGGAGCGG CTTACACCAT GGAGACTCTC
    CTGCAAAACC ACCAGCTTCG AGACCACAAC ATTCGACCTG GAGAAAGTGC CATAGTGAAG
    AAAAAGGCTG AACTCATTAA AGGGAATTAC AAGTGTAATG TGTGTTCTCG AACATTCTTC
    TCTGAAAATG GGCTCCGAGA ACACATGCAG ACACACTTGG GACCAGTGAA ACATTACATG
    TGCCCAATCT GTGGCGAGCG GTTTCCATCT CTTCTGACTC TTACTGAACA TAAAGTCACA
    CATAGTAAGA GCCTGGACAC CGGAAACTGC AGGATTTGCA AGATGCCTCT CCAGAGTGAG
    GAGGAATTTT TAGAGCATTG CCAGATGCAC CCTGACTTGA GGAACTCTTT GACAGGGTTC
    CGCTGTGTGG TGTGCATGCA GACTGTGACC TCCACGTTGG AACTGAAAAT CCACGGCACA
    TTCCACATGC AGAAAACTGG AAATGGTTCT GCCGTGCAGT CCACGGGGCG CGCACAGCAT
    CTCCAGAAAC TGTACAAGTG TGCTTCTTGC CTGAAGGAAT TTCGTTCAAA ACAGGATTTA
    GTGAAACTTG ACATCAATGG TCTGCCATAT GGTCTGTGTG CTAGCTGTGT TAATCTCAGT
    AAAAGTGGTA GTCCAAGTGT CAACATCCCA TCAAGCAGTA ACAGACAAGG CATGGGCCAA
    AATGAGAACT TGAGTTCCAT TGAGAACAAA AGCAAGGCAG GGGGACTGAA GACTCGATGT
    TCTAGTTGCA ATGTTAAATT TGAATCAGAA AGTGAACTCC AAAACCATAT TCAGTCAATC
    CACAGAGAGC TTGTTCCAGA CAGCAACAGT ACACAGCTGA AAACACCACA AGTATCTCCA
    ATGCCCAGAA TCAGCCCCTC CCAAACTGAA GAGAAGAAGA CCTACCAGTG CATCAAATGT
    CAGATGGTTT TCTACAATGA ATGGGATATC CAAGTTCATG TTGCAAACCA CATGATTGAT
    GAAGGACTGA ACCATGAATG CAAGCTCTGC AATCAGACAT TTGACTCTCC TGCCAAACTT
    CAGTGCCACC TGATAGAGCA CAGCTTTGAA GGCATGGGGG GCACCTTCAA ATGTCCAGTG
    TGCTTTACAG TGTTTGTCCA AGCAAACAAG TTGCAGCAGC ACATTTTCTC AGCCCATGGA
    CAAGAGGACA AGATCTATGA CTGCACACAG TGTCCACAGA AGTTCTTCTT CCAAACAGAG
    CTGCAGAATC ATACGATGAC CCAGCATAGC AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.