NPC1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	NPC1
Entrez Gene ID	421076
Full Name	NPC intracellular cholesterol transporter 1
General protein information	Preferred Names NPC intracellular cholesterol transporter 1 Names Niemann-Pick C1 protein Nasopharyngeal carcinoma 1 Niemann-Pick disease, type C1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004939784.2	XP_004939841.1	Niemann-Pick C1 protein isoform X2
XM_419162.5	XP_419162.3	Niemann-Pick C1 protein isoform X3
XM_419162.6	XP_419162.3	NPC intracellular cholesterol transporter 1 isoform X3
XM_004939783.3	XP_004939840.2	NPC intracellular cholesterol transporter 1 isoform X1
XM_004939783.2	XP_004939840.2	Niemann-Pick C1 protein isoform X1
XM_004939784.3	XP_004939841.1	NPC intracellular cholesterol transporter 1 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NPC1	NP_001003107.1
Bos taurus (cattle)	NPC1	NP_777183.1
Kluyveromyces lactis	KLLA0F27137g	XP_456287.1
Magnaporthe oryzae (rice blast fungus)	MGG_00819	XP_003718164.1
Mus musculus (house mouse)	Npc1	NP_032746.2
Drosophila melanogaster (fruit fly)	Npc1a	NP_001188769.1
Homo sapiens (human)	NPC1	NP_000262.2
Pan troglodytes (chimpanzee)	NPC1	XP_001155285.1
	NPC1	XP_002800934.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008137	XP_317322.4
Rattus norvegicus (Norway rat)	Npc1	NP_705888.2
Danio rerio (zebrafish)	npc1	NP_001230804.1
Saccharomyces cerevisiae (baker's yeast)	NCR1	NP_015319.1
Eremothecium gossypii	AGOS_AGL008W	NP_986658.1
Neurospora crassa	NCU04090	XP_956086.1
Arabidopsis thaliana (thale cress)	AT1G42470	NP_174975.2
Gallus gallus (chicken)	NPC1	XP_419162.3
Arabidopsis thaliana (thale cress)	AT4G38350	NP_001190953.1
Xenopus tropicalis (tropical clawed frog)	npc1	XP_004915269.1

Gene Ontology

The following NPC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa32502	XM_004939783.3 Latest version!	Gallus gallus NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa05147	XM_004939784.3 Latest version!	Gallus gallus NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa05149	XM_419162.6 Latest version!	Gallus gallus NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
OGa05149	XM_419162.5	Gallus gallus Niemann-Pick disease, type C1 (NPC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa32502	XM_004939783.2	Gallus gallus Niemann-Pick disease, type C1 (NPC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa05147	XM_004939784.2	Gallus gallus Niemann-Pick disease, type C1 (NPC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NPC1 ( XM_004939783.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004939840.2 Gallus gallus NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X1, mRNA.

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CloneID	OGa32502
Clone ID Related Accession (Same CDS sequence)	XM_004939783.3 , XM_004939783.2
Accession Version	XM_004939783.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	NPC intracellular cholesterol transporter 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004939783.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTGT TTCAACGTGC CACTGAAGAA ACTTGGACTG AAGTACTTCC ACAACAGTGT 
GTCTGGTACG GAGAATGTGG AGTTGCATCT GGAGACAAGA GATACAACTG TGCTTATGAT 
GGACCACCTA TAGCTCTACC AAAGGGTGGC TATGACTTGA TGCAGGAGCT CTGTCCAGGT 
TTATTCTTTG GCAATGTCAG TACTTGCTGT GATATCCTTC AGCTTCAGAC TTTGAAAAAC 
AACTTGCAGC TGCCTCTGCA GTTTCTTTCC AGGTGTCCAT CATGTTTCTA CAACTTGATT 
AACCTCTTCT GTGAACTGAC TTGCAGTCCA AATCAGTCTG AGTTTTTGAA TGTTACAAGC 
ACCATACCTT ATTATGATCC AGTTTCGAAA GAAAACAAAA GCAGTATTAC AGAGCTGCAG 
TACTTCATTG GAGAACGCTT TGCAAATGAA ATGTATAATG CCTGCAAAGA TGTTGAGGCT 
CCATCAAGTA ATGTTAAGGC ATTGGGGCTG CTGTGTGGGA AGGACGTCAA GGACTGCAAT 
GCTACCAACT GGATTGAGTA CATGTTTAAT AAGGATAACG GACAAACTCC TTTCAGCATT 
ATTCCTATCT TTTCAGATGC TCCTGTCCAT GGAATGAATC CTATGAACAA TGCTACCAAA 
GGCTGTAATG AGTCTGTGGA TGATTCAACA GGACCATGCA GCTGTCAAGA CTGTTCAATT 
GTTTGTGGTC CAAAACCTCA GCCCCCTCCA TTACCTGCTC CTTGGCTTTT ATTTGGTTTG 
GATGCTGTGT ATATCATCAT GTGGATCTCT TACATGGGGT TTCTACTCAT ATTTTTTGCT 
CTAGTTTTTG GAGTCTGGTG TTACAGGAGG CGGCATTTTG TCTCTGAGTA TACACCAATT 
GACAGTAATG TAGCCTTTTC TGCGAACTCT CATCGTGATA ATGGGAAAAT TACCTGTGGT 
GAGAGGCTTG GTGAAAGGTT TGAGAATGGC CTAAGGATGA CATTTACATC ATGGGGTGCT 
TTCTGTGTCA GAAATCCGCG CCCGGTTATC CTCTTCTCTG TGGTCTTTAT TGCTATGTGC 
TGTTCAGGCT TTGTATATAT TAAAGCAACT ACAAACCCTG TAGATCTCTG GTCAGCTCCA 
AGCAGCCAAG CCCGCAAGGA GAAGGAGTAC TTTGACAAAC ACTTTGGGCC TTTCTTTCGT 
ACTGAGCAAC TTATTATTCA AGCGCCAAAC AGCCATCCAG ACATCTATTC ACCTTACCCA 
TCAGGAGCAG ATGTGCCTTT TGGACCTCCA CTCAACAAAG ATATTCTCCA TCAGGTGCTT 
GATTTGCAAG ATGCTATTGT GAACATAGCT GCGTCTTTTG ACAATGAAAC TGTTACGCTG 
AAAGACATTT GCTTGGCTCC TCTTGCTCCC TACAACAACA ACTGCACTAT ATTGAGTGTA 
CTCAACTACT TCCAAAACAG TCATTCTGTT CTAGATCACA GTGTGGGGGA TGACTTCTTT 
GTCTATGCTG ACTATCACAC TCACTTCTTA TATTGTGTTC GGGCTCCAGC ATCACTGAAT 
GACACAAGTT TGCTTCACGA TCCTTGTTTA GGAGCATTTG GTGGACCTGT CTTTCCATGG 
CTGGTGTTAG GGGGATACGA TGGTGATAAC TATAATAATG CTACTGCTCT TGTTATAACT 
CTTCCTGTTA ATAACTACTA CAATGACTCC AAGAAGATAA TGAAAGCCCT GGCGTGGGAA 
AAGGAGTTTA TTAACTTTTT GAAGAACTAT GATAATCCAA ATTTGACTAT ATCATTTTCT 
GCGGAACGGA GCATTGAAGA CGAAATCAAT CGTGAAAGCA GGAGTGATGT CAGCACTGTT 
GTAATAAGCT ATGTTGTAAT GTTTGTGTAT ATCTCCATAG CTTTGGGACA TATCCAGAGC 
TGGGGAAGGC TGCTGGTGGA TTCAAAGATC TCCCTGGGCA TTGCAGGCAT CCTGATCGTC 
CTGAGCTCAG TGGCATGTTC CATAGGCATT TTCAGTTACT TTGGAGTTCC AGTGACACTG 
ATTGTGGCAG AAGTTATTCC CTTCTTGGTG CTGGCTATTG GGGTGGACAA CTTATTTATT 
ATAGTCCAGA CACTTCAGAG AGATGAGCGT CTTGAGGGTG AAACTCTTGA TAAGCAGATT 
GGCAGAGTCC TGGGAGATGT GGCACCTAGC ATGTTTCTCT CATCTTTTTC TGAGACCGTG 
GCATTCTTCT TAGGGACACT GTCTACCATG CCAGCAGTCC GCACGTTCTC CCTGTTTGCT 
GGAATGGCTG TGCTCATAGA TTTTATCCTT CAAGTCACCT GCTTTGTAAG TCTCCTGGGC 
TTGGATATTA GGCGCCAAGA GAGGAATAGA CTAGATATTC TGTGTTGCAT CAAAGGCAGT 
GAAGAAATGA GGGGAGTCCA ACGCTCCGAG AGCATCTTAT TTCTGTTCTT CAAAAACCTC 
TATTCTCCAT ATTTGCTTAA GGATTGGATG AGACCAATAG TGATTGCCTT GTTTGTGGGT 
CTTCTGTCAT TCAGTACAGC AGTGATACAC AATGTAGAGA TTGGACTCGA TCAGTCTCTT 
TCAATGCCAG ATGATTCCTA TGTAATGAAT TACTTCAAAC AGTTGGGCAA ATACATGCAT 
GCAGGTCCTC CTGTCTACTT TGTTCTAGAA GAAGGGCACA ACTACACCTC TCTGGAAGGG 
CAAAACATGG TGTGTGGTGG AACGGGATGT AATAATGATT CACTTGTCCA GCAAGTCTTC 
AATGCTGCAG AAATTAGTAG CTACACAAGG ATAGGCTATG CCCCATCATC CTGGATTGAT 
GACTATTTTG ACTGGGTGAA ACCACAGTCA TCCTGTTGCA GAGTCTACAA TACCACGGGA 
CAGTTCTGCA ATGCTTCAGT CACAGATCCA TCCTGCACTC GTTGTCGACC ACTGAGTCAA 
GAAGGCAAGC AAAGACCACA GGGTGAAGAC TTCATGACAT TTTTGCCAAT GTTCCTGTCT 
GACAACCCTA ATCCTAAATG TGGCAAAGGA GGACATGCTG CATATAGCTC AGCTGTTGAC 
TTGATAAAGA ACAAAACTGA TGTTGGGGCT ACTTATTTTA TGACGTACCA TACTGTCCTG 
AAAAAATCAT CTGACTATAT TGATGCTATG AAGAAGGCTC GGGACATAGC AGACAACATC 
ACTGAAACCA TGGGCATCAA AGAGAAGAAC TATCGTGTGT TTCCTTACAG TGTGTTTTAT 
GTCTTCTATG AACAATATTT GACAATTGTG AATGATGCCA TCTTTAACCT CTGCATCTCC 
TTGGGATCGA TATTTTTGGT GACAGCTGTG CTGCTTGGGT TTGAAGTATG GGCTGCTGTT 
GTGGTTACCG TAACGATTGC TATGATAATT GTCAACATGT TTGGAGTGAT GTGGCTCTGG 
GGCATCAGCT TGAATGCAGT TTCATTAGTA AATCTCGTCA TGAGCTGTGG CATTGCTGTG 
GAATTCTGCA GCCACGTGAC GAGAGCATTC ACTGTTAGTA CTAAAGGCAG TAGAGTGGAG 
CGTGCAGAAG AAGCTCTGTC TCACATGGGC AGTTCAGTCT TCAGTGGAAT CACACTAACC 
AAGTTTGGAG GAATTGTAGT ACTGGCTTTT TCCAAGTCTC AGATCTTCAA GATATTCTAC 
TTCAGGATGT ATCTAGCTAT GGTTCTGCTG GGAGCAACGC ATGGACTAAT ATTTCTTCCA 
GTTCTGCTTA GTTACATAGG TCCATCAGTA AATAAAGCTA AAACTCGTGC TGCGCAGGAG 
AGGTCTAGAG GCACAGAACG AGAGAGACTC CTCTACTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004939840.2
CDS	16..3837
Translation	MDLFQRATEETWTEVLPQQCVWYGECGVASGDKRYNCAYDGPPIALPKGGYDLMQELCPGLFFGNVSTCCDILQLQTLKNNLQLPLQFLSRCPSCFYNLINLFCELTCSPNQSEFLNVTSTIPYYDPVSKENKSSITELQYFIGERFANEMYNACKDVEAPSSNVKALGLLCGKDVKDCNATNWIEYMFNKDNGQTPFSIIPIFSDAPVHGMNPMNNATKGCNESVDDSTGPCSCQDCSIVCGPKPQPPPLPAPWLLFGLDAVYIIMWISYMGFLLIFFALVFGVWCYRRRHFVSEYTPIDSNVAFSANSHRDNGKITCGERLGERFENGLRMTFTSWGAFCVRNPRPVILFSVVFIAMCCSGFVYIKATTNPVDLWSAPSSQARKEKEYFDKHFGPFFRTEQLIIQAPNSHPDIYSPYPSGADVPFGPPLNKDILHQVLDLQDAIVNIAASFDNETVTLKDICLAPLAPYNNNCTILSVLNYFQNSHSVLDHSVGDDFFVYADYHTHFLYCVRAPASLNDTSLLHDPCLGAFGGPVFPWLVLGGYDGDNYNNATALVITLPVNNYYNDSKKIMKALAWEKEFINFLKNYDNPNLTISFSAERSIEDEINRESRSDVSTVVISYVVMFVYISIALGHIQSWGRLLVDSKISLGIAGILIVLSSVACSIGIFSYFGVPVTLIVAEVIPFLVLAIGVDNLFIIVQTLQRDERLEGETLDKQIGRVLGDVAPSMFLSSFSETVAFFLGTLSTMPAVRTFSLFAGMAVLIDFILQVTCFVSLLGLDIRRQERNRLDILCCIKGSEEMRGVQRSESILFLFFKNLYSPYLLKDWMRPIVIALFVGLLSFSTAVIHNVEIGLDQSLSMPDDSYVMNYFKQLGKYMHAGPPVYFVLEEGHNYTSLEGQNMVCGGTGCNNDSLVQQVFNAAEISSYTRIGYAPSSWIDDYFDWVKPQSSCCRVYNTTGQFCNASVTDPSCTRCRPLSQEGKQRPQGEDFMTFLPMFLSDNPNPKCGKGGHAAYSSAVDLIKNKTDVGATYFMTYHTVLKKSSDYIDAMKKARDIADNITETMGIKEKNYRVFPYSVFYVFYEQYLTIVNDAIFNLCISLGSIFLVTAVLLGFEVWAAVVVTVTIAMIIVNMFGVMWLWGISLNAVSLVNLVMSCGIAVEFCSHVTRAFTVSTKGSRVERAEEALSHMGSSVFSGITLTKFGGIVVLAFSKSQIFKIFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAKTRAAQERSRGTERERLLYF

Target ORF information:

RefSeq Version	XM_004939783.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004939783.3

ORF Insert Sequence:

ATGGATTTGT TTCAACGTGC CACTGAAGAA ACTTGGACTG AAGTACTTCC ACAACAGTGT 
GTCTGGTACG GAGAATGTGG AGTTGCATCT GGAGACAAGA GATACAACTG TGCTTATGAT 
GGACCACCTA TAGCTCTACC AAAGGGTGGC TATGACTTGA TGCAGGAGCT CTGTCCAGGT 
TTATTCTTTG GCAATGTCAG TACTTGCTGT GATATCCTTC AGCTTCAGAC TTTGAAAAAC 
AACTTGCAGC TGCCTCTGCA GTTTCTTTCC AGGTGTCCAT CATGTTTCTA CAACTTGATT 
AACCTCTTCT GTGAACTGAC TTGCAGTCCA AATCAGTCTG AGTTTTTGAA TGTTACAAGC 
ACCATACCTT ATTATGATCC AGTTTCGAAA GAAAACAAAA GCAGTATTAC AGAGCTGCAG 
TACTTCATTG GAGAACGCTT TGCAAATGAA ATGTATAATG CCTGCAAAGA TGTTGAGGCT 
CCATCAAGTA ATGTTAAGGC ATTGGGGCTG CTGTGTGGGA AGGACGTCAA GGACTGCAAT 
GCTACCAACT GGATTGAGTA CATGTTTAAT AAGGATAACG GACAAACTCC TTTCAGCATT 
ATTCCTATCT TTTCAGATGC TCCTGTCCAT GGAATGAATC CTATGAACAA TGCTACCAAA 
GGCTGTAATG AGTCTGTGGA TGATTCAACA GGACCATGCA GCTGTCAAGA CTGTTCAATT 
GTTTGTGGTC CAAAACCTCA GCCCCCTCCA TTACCTGCTC CTTGGCTTTT ATTTGGTTTG 
GATGCTGTGT ATATCATCAT GTGGATCTCT TACATGGGGT TTCTACTCAT ATTTTTTGCT 
CTAGTTTTTG GAGTCTGGTG TTACAGGAGG CGGCATTTTG TCTCTGAGTA TACACCAATT 
GACAGTAATG TAGCCTTTTC TGCGAACTCT CATCGTGATA ATGGGAAAAT TACCTGTGGT 
GAGAGGCTTG GTGAAAGGTT TGAGAATGGC CTAAGGATGA CATTTACATC ATGGGGTGCT 
TTCTGTGTCA GAAATCCGCG CCCGGTTATC CTCTTCTCTG TGGTCTTTAT TGCTATGTGC 
TGTTCAGGCT TTGTATATAT TAAAGCAACT ACAAACCCTG TAGATCTCTG GTCAGCTCCA 
AGCAGCCAAG CCCGCAAGGA GAAGGAGTAC TTTGACAAAC ACTTTGGGCC TTTCTTTCGT 
ACTGAGCAAC TTATTATTCA AGCGCCAAAC AGCCATCCAG ACATCTATTC ACCTTACCCA 
TCAGGAGCAG ATGTGCCTTT TGGACCTCCA CTCAACAAAG ATATTCTCCA TCAGGTGCTT 
GATTTGCAAG ATGCTATTGT GAACATAGCT GCGTCTTTTG ACAATGAAAC TGTTACGCTG 
AAAGACATTT GCTTGGCTCC TCTTGCTCCC TACAACAACA ACTGCACTAT ATTGAGTGTA 
CTCAACTACT TCCAAAACAG TCATTCTGTT CTAGATCACA GTGTGGGGGA TGACTTCTTT 
GTCTATGCTG ACTATCACAC TCACTTCTTA TATTGTGTTC GGGCTCCAGC ATCACTGAAT 
GACACAAGTT TGCTTCACGA TCCTTGTTTA GGAGCATTTG GTGGACCTGT CTTTCCATGG 
CTGGTGTTAG GGGGATACGA TGGTGATAAC TATAATAATG CTACTGCTCT TGTTATAACT 
CTTCCTGTTA ATAACTACTA CAATGACTCC AAGAAGATAA TGAAAGCCCT GGCGTGGGAA 
AAGGAGTTTA TTAACTTTTT GAAGAACTAT GATAATCCAA ATTTGACTAT ATCATTTTCT 
GCGGAACGGA GCATTGAAGA CGAAATCAAT CGTGAAAGCA GGAGTGATGT CAGCACTGTT 
GTAATAAGCT ATGTTGTAAT GTTTGTGTAT ATCTCCATAG CTTTGGGACA TATCCAGAGC 
TGGGGAAGGC TGCTGGTGGA TTCAAAGATC TCCCTGGGCA TTGCAGGCAT CCTGATCGTC 
CTGAGCTCAG TGGCATGTTC CATAGGCATT TTCAGTTACT TTGGAGTTCC AGTGACACTG 
ATTGTGGCAG AAGTTATTCC CTTCTTGGTG CTGGCTATTG GGGTGGACAA CTTATTTATT 
ATAGTCCAGA CACTTCAGAG AGATGAGCGT CTTGAGGGTG AAACTCTTGA TAAGCAGATT 
GGCAGAGTCC TGGGAGATGT GGCACCTAGC ATGTTTCTCT CATCTTTTTC TGAGACCGTG 
GCATTCTTCT TAGGGACACT GTCTACCATG CCAGCAGTCC GCACGTTCTC CCTGTTTGCT 
GGAATGGCTG TGCTCATAGA TTTTATCCTT CAAGTCACCT GCTTTGTAAG TCTCCTGGGC 
TTGGATATTA GGCGCCAAGA GAGGAATAGA CTAGATATTC TGTGTTGCAT CAAAGGCAGT 
GAAGAAATGA GGGGAGTCCA ACGCTCCGAG AGCATCTTAT TTCTGTTCTT CAAAAACCTC 
TATTCTCCAT ATTTGCTTAA GGATTGGATG AGACCAATAG TGATTGCCTT GTTTGTGGGT 
CTTCTGTCAT TCAGTACAGC AGTGATACAC AATGTAGAGA TTGGACTCGA TCAGTCTCTT 
TCAATGCCAG ATGATTCCTA TGTAATGAAT TACTTCAAAC AGTTGGGCAA ATACATGCAT 
GCAGGTCCTC CTGTCTACTT TGTTCTAGAA GAAGGGCACA ACTACACCTC TCTGGAAGGG 
CAAAACATGG TGTGTGGTGG AACGGGATGT AATAATGATT CACTTGTCCA GCAAGTCTTC 
AATGCTGCAG AAATTAGTAG CTACACAAGG ATAGGCTATG CCCCATCATC CTGGATTGAT 
GACTATTTTG ACTGGGTGAA ACCACAGTCA TCCTGTTGCA GAGTCTACAA TACCACGGGA 
CAGTTCTGCA ATGCTTCAGT CACAGATCCA TCCTGCACTC GTTGTCGACC ACTGAGTCAA 
GAAGGCAAGC AAAGACCACA GGGTGAAGAC TTCATGACAT TTTTGCCAAT GTTCCTGTCT 
GACAACCCTA ATCCTAAATG TGGCAAAGGA GGACATGCTG CATATAGCTC AGCTGTTGAC 
TTGATAAAGA ACAAAACTGA TGTTGGGGCT ACTTATTTTA TGACGTACCA TACTGTCCTG 
AAAAAATCAT CTGACTATAT TGATGCTATG AAGAAGGCTC GGGACATAGC AGACAACATC 
ACTGAAACCA TGGGCATCAA AGAGAAGAAC TATCGTGTGT TTCCTTACAG TGTGTTTTAT 
GTCTTCTATG AACAATATTT GACAATTGTG AATGATGCCA TCTTTAACCT CTGCATCTCC 
TTGGGATCGA TATTTTTGGT GACAGCTGTG CTGCTTGGGT TTGAAGTATG GGCTGCTGTT 
GTGGTTACCG TAACGATTGC TATGATAATT GTCAACATGT TTGGAGTGAT GTGGCTCTGG 
GGCATCAGCT TGAATGCAGT TTCATTAGTA AATCTCGTCA TGAGCTGTGG CATTGCTGTG 
GAATTCTGCA GCCACGTGAC GAGAGCATTC ACTGTTAGTA CTAAAGGCAG TAGAGTGGAG 
CGTGCAGAAG AAGCTCTGTC TCACATGGGC AGTTCAGTCT TCAGTGGAAT CACACTAACC 
AAGTTTGGAG GAATTGTAGT ACTGGCTTTT TCCAAGTCTC AGATCTTCAA GATATTCTAC 
TTCAGGATGT ATCTAGCTAT GGTTCTGCTG GGAGCAACGC ATGGACTAAT ATTTCTTCCA 
GTTCTGCTTA GTTACATAGG TCCATCAGTA AATAAAGCTA AAACTCGTGC TGCGCAGGAG 
AGGTCTAGAG GCACAGAACG AGAGAGACTC CTCTACTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPC1 ( XM_004939783.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004939840.2 Gallus gallus Niemann-Pick disease, type C1 (NPC1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa32502
Clone ID Related Accession (Same CDS sequence)	XM_004939783.3 , XM_004939783.2
Accession Version	XM_004939783.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	Niemann-Pick C1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004939783.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTGT TTCAACGTGC CACTGAAGAA ACTTGGACTG AAGTACTTCC ACAACAGTGT 
GTCTGGTACG GAGAATGTGG AGTTGCATCT GGAGACAAGA GATACAACTG TGCTTATGAT 
GGACCACCTA TAGCTCTACC AAAGGGTGGC TATGACTTGA TGCAGGAGCT CTGTCCAGGT 
TTATTCTTTG GCAATGTCAG TACTTGCTGT GATATCCTTC AGCTTCAGAC TTTGAAAAAC 
AACTTGCAGC TGCCTCTGCA GTTTCTTTCC AGGTGTCCAT CATGTTTCTA CAACTTGATT 
AACCTCTTCT GTGAACTGAC TTGCAGTCCA AATCAGTCTG AGTTTTTGAA TGTTACAAGC 
ACCATACCTT ATTATGATCC AGTTTCGAAA GAAAACAAAA GCAGTATTAC AGAGCTGCAG 
TACTTCATTG GAGAACGCTT TGCAAATGAA ATGTATAATG CCTGCAAAGA TGTTGAGGCT 
CCATCAAGTA ATGTTAAGGC ATTGGGGCTG CTGTGTGGGA AGGACGTCAA GGACTGCAAT 
GCTACCAACT GGATTGAGTA CATGTTTAAT AAGGATAACG GACAAACTCC TTTCAGCATT 
ATTCCTATCT TTTCAGATGC TCCTGTCCAT GGAATGAATC CTATGAACAA TGCTACCAAA 
GGCTGTAATG AGTCTGTGGA TGATTCAACA GGACCATGCA GCTGTCAAGA CTGTTCAATT 
GTTTGTGGTC CAAAACCTCA GCCCCCTCCA TTACCTGCTC CTTGGCTTTT ATTTGGTTTG 
GATGCTGTGT ATATCATCAT GTGGATCTCT TACATGGGGT TTCTACTCAT ATTTTTTGCT 
CTAGTTTTTG GAGTCTGGTG TTACAGGAGG CGGCATTTTG TCTCTGAGTA TACACCAATT 
GACAGTAATG TAGCCTTTTC TGCGAACTCT CATCGTGATA ATGGGAAAAT TACCTGTGGT 
GAGAGGCTTG GTGAAAGGTT TGAGAATGGC CTAAGGATGA CATTTACATC ATGGGGTGCT 
TTCTGTGTCA GAAATCCGCG CCCGGTTATC CTCTTCTCTG TGGTCTTTAT TGCTATGTGC 
TGTTCAGGCT TTGTATATAT TAAAGCAACT ACAAACCCTG TAGATCTCTG GTCAGCTCCA 
AGCAGCCAAG CCCGCAAGGA GAAGGAGTAC TTTGACAAAC ACTTTGGGCC TTTCTTTCGT 
ACTGAGCAAC TTATTATTCA AGCGCCAAAC AGCCATCCAG ACATCTATTC ACCTTACCCA 
TCAGGAGCAG ATGTGCCTTT TGGACCTCCA CTCAACAAAG ATATTCTCCA TCAGGTGCTT 
GATTTGCAAG ATGCTATTGT GAACATAGCT GCGTCTTTTG ACAATGAAAC TGTTACGCTG 
AAAGACATTT GCTTGGCTCC TCTTGCTCCC TACAACAACA ACTGCACTAT ATTGAGTGTA 
CTCAACTACT TCCAAAACAG TCATTCTGTT CTAGATCACA GTGTGGGGGA TGACTTCTTT 
GTCTATGCTG ACTATCACAC TCACTTCTTA TATTGTGTTC GGGCTCCAGC ATCACTGAAT 
GACACAAGTT TGCTTCACGA TCCTTGTTTA GGAGCATTTG GTGGACCTGT CTTTCCATGG 
CTGGTGTTAG GGGGATACGA TGGTGATAAC TATAATAATG CTACTGCTCT TGTTATAACT 
CTTCCTGTTA ATAACTACTA CAATGACTCC AAGAAGATAA TGAAAGCCCT GGCGTGGGAA 
AAGGAGTTTA TTAACTTTTT GAAGAACTAT GATAATCCAA ATTTGACTAT ATCATTTTCT 
GCGGAACGGA GCATTGAAGA CGAAATCAAT CGTGAAAGCA GGAGTGATGT CAGCACTGTT 
GTAATAAGCT ATGTTGTAAT GTTTGTGTAT ATCTCCATAG CTTTGGGACA TATCCAGAGC 
TGGGGAAGGC TGCTGGTGGA TTCAAAGATC TCCCTGGGCA TTGCAGGCAT CCTGATCGTC 
CTGAGCTCAG TGGCATGTTC CATAGGCATT TTCAGTTACT TTGGAGTTCC AGTGACACTG 
ATTGTGGCAG AAGTTATTCC CTTCTTGGTG CTGGCTATTG GGGTGGACAA CTTATTTATT 
ATAGTCCAGA CACTTCAGAG AGATGAGCGT CTTGAGGGTG AAACTCTTGA TAAGCAGATT 
GGCAGAGTCC TGGGAGATGT GGCACCTAGC ATGTTTCTCT CATCTTTTTC TGAGACCGTG 
GCATTCTTCT TAGGGACACT GTCTACCATG CCAGCAGTCC GCACGTTCTC CCTGTTTGCT 
GGAATGGCTG TGCTCATAGA TTTTATCCTT CAAGTCACCT GCTTTGTAAG TCTCCTGGGC 
TTGGATATTA GGCGCCAAGA GAGGAATAGA CTAGATATTC TGTGTTGCAT CAAAGGCAGT 
GAAGAAATGA GGGGAGTCCA ACGCTCCGAG AGCATCTTAT TTCTGTTCTT CAAAAACCTC 
TATTCTCCAT ATTTGCTTAA GGATTGGATG AGACCAATAG TGATTGCCTT GTTTGTGGGT 
CTTCTGTCAT TCAGTACAGC AGTGATACAC AATGTAGAGA TTGGACTCGA TCAGTCTCTT 
TCAATGCCAG ATGATTCCTA TGTAATGAAT TACTTCAAAC AGTTGGGCAA ATACATGCAT 
GCAGGTCCTC CTGTCTACTT TGTTCTAGAA GAAGGGCACA ACTACACCTC TCTGGAAGGG 
CAAAACATGG TGTGTGGTGG AACGGGATGT AATAATGATT CACTTGTCCA GCAAGTCTTC 
AATGCTGCAG AAATTAGTAG CTACACAAGG ATAGGCTATG CCCCATCATC CTGGATTGAT 
GACTATTTTG ACTGGGTGAA ACCACAGTCA TCCTGTTGCA GAGTCTACAA TACCACGGGA 
CAGTTCTGCA ATGCTTCAGT CACAGATCCA TCCTGCACTC GTTGTCGACC ACTGAGTCAA 
GAAGGCAAGC AAAGACCACA GGGTGAAGAC TTCATGACAT TTTTGCCAAT GTTCCTGTCT 
GACAACCCTA ATCCTAAATG TGGCAAAGGA GGACATGCTG CATATAGCTC AGCTGTTGAC 
TTGATAAAGA ACAAAACTGA TGTTGGGGCT ACTTATTTTA TGACGTACCA TACTGTCCTG 
AAAAAATCAT CTGACTATAT TGATGCTATG AAGAAGGCTC GGGACATAGC AGACAACATC 
ACTGAAACCA TGGGCATCAA AGAGAAGAAC TATCGTGTGT TTCCTTACAG TGTGTTTTAT 
GTCTTCTATG AACAATATTT GACAATTGTG AATGATGCCA TCTTTAACCT CTGCATCTCC 
TTGGGATCGA TATTTTTGGT GACAGCTGTG CTGCTTGGGT TTGAAGTATG GGCTGCTGTT 
GTGGTTACCG TAACGATTGC TATGATAATT GTCAACATGT TTGGAGTGAT GTGGCTCTGG 
GGCATCAGCT TGAATGCAGT TTCATTAGTA AATCTCGTCA TGAGCTGTGG CATTGCTGTG 
GAATTCTGCA GCCACGTGAC GAGAGCATTC ACTGTTAGTA CTAAAGGCAG TAGAGTGGAG 
CGTGCAGAAG AAGCTCTGTC TCACATGGGC AGTTCAGTCT TCAGTGGAAT CACACTAACC 
AAGTTTGGAG GAATTGTAGT ACTGGCTTTT TCCAAGTCTC AGATCTTCAA GATATTCTAC 
TTCAGGATGT ATCTAGCTAT GGTTCTGCTG GGAGCAACGC ATGGACTAAT ATTTCTTCCA 
GTTCTGCTTA GTTACATAGG TCCATCAGTA AATAAAGCTA AAACTCGTGC TGCGCAGGAG 
AGGTCTAGAG GCACAGAACG AGAGAGACTC CTCTACTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004939840.2
CDS	16..3837
Translation	MDLFQRATEETWTEVLPQQCVWYGECGVASGDKRYNCAYDGPPIALPKGGYDLMQELCPGLFFGNVSTCCDILQLQTLKNNLQLPLQFLSRCPSCFYNLINLFCELTCSPNQSEFLNVTSTIPYYDPVSKENKSSITELQYFIGERFANEMYNACKDVEAPSSNVKALGLLCGKDVKDCNATNWIEYMFNKDNGQTPFSIIPIFSDAPVHGMNPMNNATKGCNESVDDSTGPCSCQDCSIVCGPKPQPPPLPAPWLLFGLDAVYIIMWISYMGFLLIFFALVFGVWCYRRRHFVSEYTPIDSNVAFSANSHRDNGKITCGERLGERFENGLRMTFTSWGAFCVRNPRPVILFSVVFIAMCCSGFVYIKATTNPVDLWSAPSSQARKEKEYFDKHFGPFFRTEQLIIQAPNSHPDIYSPYPSGADVPFGPPLNKDILHQVLDLQDAIVNIAASFDNETVTLKDICLAPLAPYNNNCTILSVLNYFQNSHSVLDHSVGDDFFVYADYHTHFLYCVRAPASLNDTSLLHDPCLGAFGGPVFPWLVLGGYDGDNYNNATALVITLPVNNYYNDSKKIMKALAWEKEFINFLKNYDNPNLTISFSAERSIEDEINRESRSDVSTVVISYVVMFVYISIALGHIQSWGRLLVDSKISLGIAGILIVLSSVACSIGIFSYFGVPVTLIVAEVIPFLVLAIGVDNLFIIVQTLQRDERLEGETLDKQIGRVLGDVAPSMFLSSFSETVAFFLGTLSTMPAVRTFSLFAGMAVLIDFILQVTCFVSLLGLDIRRQERNRLDILCCIKGSEEMRGVQRSESILFLFFKNLYSPYLLKDWMRPIVIALFVGLLSFSTAVIHNVEIGLDQSLSMPDDSYVMNYFKQLGKYMHAGPPVYFVLEEGHNYTSLEGQNMVCGGTGCNNDSLVQQVFNAAEISSYTRIGYAPSSWIDDYFDWVKPQSSCCRVYNTTGQFCNASVTDPSCTRCRPLSQEGKQRPQGEDFMTFLPMFLSDNPNPKCGKGGHAAYSSAVDLIKNKTDVGATYFMTYHTVLKKSSDYIDAMKKARDIADNITETMGIKEKNYRVFPYSVFYVFYEQYLTIVNDAIFNLCISLGSIFLVTAVLLGFEVWAAVVVTVTIAMIIVNMFGVMWLWGISLNAVSLVNLVMSCGIAVEFCSHVTRAFTVSTKGSRVERAEEALSHMGSSVFSGITLTKFGGIVVLAFSKSQIFKIFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAKTRAAQERSRGTERERLLYF

Target ORF information:

RefSeq Version	XM_004939783.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Niemann-Pick disease, type C1 (NPC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004939783.2

ORF Insert Sequence:

ATGGATTTGT TTCAACGTGC CACTGAAGAA ACTTGGACTG AAGTACTTCC ACAACAGTGT 
GTCTGGTACG GAGAATGTGG AGTTGCATCT GGAGACAAGA GATACAACTG TGCTTATGAT 
GGACCACCTA TAGCTCTACC AAAGGGTGGC TATGACTTGA TGCAGGAGCT CTGTCCAGGT 
TTATTCTTTG GCAATGTCAG TACTTGCTGT GATATCCTTC AGCTTCAGAC TTTGAAAAAC 
AACTTGCAGC TGCCTCTGCA GTTTCTTTCC AGGTGTCCAT CATGTTTCTA CAACTTGATT 
AACCTCTTCT GTGAACTGAC TTGCAGTCCA AATCAGTCTG AGTTTTTGAA TGTTACAAGC 
ACCATACCTT ATTATGATCC AGTTTCGAAA GAAAACAAAA GCAGTATTAC AGAGCTGCAG 
TACTTCATTG GAGAACGCTT TGCAAATGAA ATGTATAATG CCTGCAAAGA TGTTGAGGCT 
CCATCAAGTA ATGTTAAGGC ATTGGGGCTG CTGTGTGGGA AGGACGTCAA GGACTGCAAT 
GCTACCAACT GGATTGAGTA CATGTTTAAT AAGGATAACG GACAAACTCC TTTCAGCATT 
ATTCCTATCT TTTCAGATGC TCCTGTCCAT GGAATGAATC CTATGAACAA TGCTACCAAA 
GGCTGTAATG AGTCTGTGGA TGATTCAACA GGACCATGCA GCTGTCAAGA CTGTTCAATT 
GTTTGTGGTC CAAAACCTCA GCCCCCTCCA TTACCTGCTC CTTGGCTTTT ATTTGGTTTG 
GATGCTGTGT ATATCATCAT GTGGATCTCT TACATGGGGT TTCTACTCAT ATTTTTTGCT 
CTAGTTTTTG GAGTCTGGTG TTACAGGAGG CGGCATTTTG TCTCTGAGTA TACACCAATT 
GACAGTAATG TAGCCTTTTC TGCGAACTCT CATCGTGATA ATGGGAAAAT TACCTGTGGT 
GAGAGGCTTG GTGAAAGGTT TGAGAATGGC CTAAGGATGA CATTTACATC ATGGGGTGCT 
TTCTGTGTCA GAAATCCGCG CCCGGTTATC CTCTTCTCTG TGGTCTTTAT TGCTATGTGC 
TGTTCAGGCT TTGTATATAT TAAAGCAACT ACAAACCCTG TAGATCTCTG GTCAGCTCCA 
AGCAGCCAAG CCCGCAAGGA GAAGGAGTAC TTTGACAAAC ACTTTGGGCC TTTCTTTCGT 
ACTGAGCAAC TTATTATTCA AGCGCCAAAC AGCCATCCAG ACATCTATTC ACCTTACCCA 
TCAGGAGCAG ATGTGCCTTT TGGACCTCCA CTCAACAAAG ATATTCTCCA TCAGGTGCTT 
GATTTGCAAG ATGCTATTGT GAACATAGCT GCGTCTTTTG ACAATGAAAC TGTTACGCTG 
AAAGACATTT GCTTGGCTCC TCTTGCTCCC TACAACAACA ACTGCACTAT ATTGAGTGTA 
CTCAACTACT TCCAAAACAG TCATTCTGTT CTAGATCACA GTGTGGGGGA TGACTTCTTT 
GTCTATGCTG ACTATCACAC TCACTTCTTA TATTGTGTTC GGGCTCCAGC ATCACTGAAT 
GACACAAGTT TGCTTCACGA TCCTTGTTTA GGAGCATTTG GTGGACCTGT CTTTCCATGG 
CTGGTGTTAG GGGGATACGA TGGTGATAAC TATAATAATG CTACTGCTCT TGTTATAACT 
CTTCCTGTTA ATAACTACTA CAATGACTCC AAGAAGATAA TGAAAGCCCT GGCGTGGGAA 
AAGGAGTTTA TTAACTTTTT GAAGAACTAT GATAATCCAA ATTTGACTAT ATCATTTTCT 
GCGGAACGGA GCATTGAAGA CGAAATCAAT CGTGAAAGCA GGAGTGATGT CAGCACTGTT 
GTAATAAGCT ATGTTGTAAT GTTTGTGTAT ATCTCCATAG CTTTGGGACA TATCCAGAGC 
TGGGGAAGGC TGCTGGTGGA TTCAAAGATC TCCCTGGGCA TTGCAGGCAT CCTGATCGTC 
CTGAGCTCAG TGGCATGTTC CATAGGCATT TTCAGTTACT TTGGAGTTCC AGTGACACTG 
ATTGTGGCAG AAGTTATTCC CTTCTTGGTG CTGGCTATTG GGGTGGACAA CTTATTTATT 
ATAGTCCAGA CACTTCAGAG AGATGAGCGT CTTGAGGGTG AAACTCTTGA TAAGCAGATT 
GGCAGAGTCC TGGGAGATGT GGCACCTAGC ATGTTTCTCT CATCTTTTTC TGAGACCGTG 
GCATTCTTCT TAGGGACACT GTCTACCATG CCAGCAGTCC GCACGTTCTC CCTGTTTGCT 
GGAATGGCTG TGCTCATAGA TTTTATCCTT CAAGTCACCT GCTTTGTAAG TCTCCTGGGC 
TTGGATATTA GGCGCCAAGA GAGGAATAGA CTAGATATTC TGTGTTGCAT CAAAGGCAGT 
GAAGAAATGA GGGGAGTCCA ACGCTCCGAG AGCATCTTAT TTCTGTTCTT CAAAAACCTC 
TATTCTCCAT ATTTGCTTAA GGATTGGATG AGACCAATAG TGATTGCCTT GTTTGTGGGT 
CTTCTGTCAT TCAGTACAGC AGTGATACAC AATGTAGAGA TTGGACTCGA TCAGTCTCTT 
TCAATGCCAG ATGATTCCTA TGTAATGAAT TACTTCAAAC AGTTGGGCAA ATACATGCAT 
GCAGGTCCTC CTGTCTACTT TGTTCTAGAA GAAGGGCACA ACTACACCTC TCTGGAAGGG 
CAAAACATGG TGTGTGGTGG AACGGGATGT AATAATGATT CACTTGTCCA GCAAGTCTTC 
AATGCTGCAG AAATTAGTAG CTACACAAGG ATAGGCTATG CCCCATCATC CTGGATTGAT 
GACTATTTTG ACTGGGTGAA ACCACAGTCA TCCTGTTGCA GAGTCTACAA TACCACGGGA 
CAGTTCTGCA ATGCTTCAGT CACAGATCCA TCCTGCACTC GTTGTCGACC ACTGAGTCAA 
GAAGGCAAGC AAAGACCACA GGGTGAAGAC TTCATGACAT TTTTGCCAAT GTTCCTGTCT 
GACAACCCTA ATCCTAAATG TGGCAAAGGA GGACATGCTG CATATAGCTC AGCTGTTGAC 
TTGATAAAGA ACAAAACTGA TGTTGGGGCT ACTTATTTTA TGACGTACCA TACTGTCCTG 
AAAAAATCAT CTGACTATAT TGATGCTATG AAGAAGGCTC GGGACATAGC AGACAACATC 
ACTGAAACCA TGGGCATCAA AGAGAAGAAC TATCGTGTGT TTCCTTACAG TGTGTTTTAT 
GTCTTCTATG AACAATATTT GACAATTGTG AATGATGCCA TCTTTAACCT CTGCATCTCC 
TTGGGATCGA TATTTTTGGT GACAGCTGTG CTGCTTGGGT TTGAAGTATG GGCTGCTGTT 
GTGGTTACCG TAACGATTGC TATGATAATT GTCAACATGT TTGGAGTGAT GTGGCTCTGG 
GGCATCAGCT TGAATGCAGT TTCATTAGTA AATCTCGTCA TGAGCTGTGG CATTGCTGTG 
GAATTCTGCA GCCACGTGAC GAGAGCATTC ACTGTTAGTA CTAAAGGCAG TAGAGTGGAG 
CGTGCAGAAG AAGCTCTGTC TCACATGGGC AGTTCAGTCT TCAGTGGAAT CACACTAACC 
AAGTTTGGAG GAATTGTAGT ACTGGCTTTT TCCAAGTCTC AGATCTTCAA GATATTCTAC 
TTCAGGATGT ATCTAGCTAT GGTTCTGCTG GGAGCAACGC ATGGACTAAT ATTTCTTCCA 
GTTCTGCTTA GTTACATAGG TCCATCAGTA AATAAAGCTA AAACTCGTGC TGCGCAGGAG 
AGGTCTAGAG GCACAGAACG AGAGAGACTC CTCTACTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPC1 ( XM_004939784.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004939841.1 Gallus gallus Niemann-Pick disease, type C1 (NPC1), transcript variant X2, mRNA.

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CloneID	OGa05147
Clone ID Related Accession (Same CDS sequence)	XM_004939784.2 , XM_004939784.3
Accession Version	XM_004939784.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	Niemann-Pick C1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004939784.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTTG TCTCAGGACT TGAACAGAGT ACTGGAAGAG ACAGCGTACT TCCACAACAG 
TGTGTCTGGT ACGGAGAATG TGGAGTTGCA TCTGGAGACA AGAGATACAA CTGTGCTTAT 
GATGGACCAC CTATAGCTCT ACCAAAGGGT GGCTATGACT TGATGCAGGA GCTCTGTCCA 
GGTTTATTCT TTGGCAATGT CAGTACTTGC TGTGATATCC TTCAGCTTCA GACTTTGAAA 
AACAACTTGC AGCTGCCTCT GCAGTTTCTT TCCAGGTGTC CATCATGTTT CTACAACTTG 
ATTAACCTCT TCTGTGAACT GACTTGCAGT CCAAATCAGT CTGAGTTTTT GAATGTTACA 
AGCACCATAC CTTATTATGA TCCAGTTTCG AAAGAAAACA AAAGCAGTAT TACAGAGCTG 
CAGTACTTCA TTGGAGAACG CTTTGCAAAT GAAATGTATA ATGCCTGCAA AGATGTTGAG 
GCTCCATCAA GTAATGTTAA GGCATTGGGG CTGCTGTGTG GGAAGGACGT CAAGGACTGC 
AATGCTACCA ACTGGATTGA GTACATGTTT AATAAGGATA ACGGACAAAC TCCTTTCAGC 
ATTATTCCTA TCTTTTCAGA TGCTCCTGTC CATGGAATGA ATCCTATGAA CAATGCTACC 
AAAGGCTGTA ATGAGTCTGT GGATGATTCA ACAGGACCAT GCAGCTGTCA AGACTGTTCA 
ATTGTTTGTG GTCCAAAACC TCAGCCCCCT CCATTACCTG CTCCTTGGCT TTTATTTGGT 
TTGGATGCTG TGTATATCAT CATGTGGATC TCTTACATGG GGTTTCTACT CATATTTTTT 
GCTCTAGTTT TTGGAGTCTG GTGTTACAGG AGGCGGCATT TTGTCTCTGA GTATACACCA 
ATTGACAGTA ATGTAGCCTT TTCTGCGAAC TCTCATCGTG ATAATGGGAA AATTACCTGT 
GGTGAGAGGC TTGGTGAAAG GTTTGAGAAT GGCCTAAGGA TGACATTTAC ATCATGGGGT 
GCTTTCTGTG TCAGAAATCC GCGCCCGGTT ATCCTCTTCT CTGTGGTCTT TATTGCTATG 
TGCTGTTCAG GCTTTGTATA TATTAAAGCA ACTACAAACC CTGTAGATCT CTGGTCAGCT 
CCAAGCAGCC AAGCCCGCAA GGAGAAGGAG TACTTTGACA AACACTTTGG GCCTTTCTTT 
CGTACTGAGC AACTTATTAT TCAAGCGCCA AACAGCCATC CAGACATCTA TTCACCTTAC 
CCATCAGGAG CAGATGTGCC TTTTGGACCT CCACTCAACA AAGATATTCT CCATCAGGTG 
CTTGATTTGC AAGATGCTAT TGTGAACATA GCTGCGTCTT TTGACAATGA AACTGTTACG 
CTGAAAGACA TTTGCTTGGC TCCTCTTGCT CCCTACAACA ACAACTGCAC TATATTGAGT 
GTACTCAACT ACTTCCAAAA CAGTCATTCT GTTCTAGATC ACAGTGTGGG GGATGACTTC 
TTTGTCTATG CTGACTATCA CACTCACTTC TTATATTGTG TTCGGGCTCC AGCATCACTG 
AATGACACAA GTTTGCTTCA CGATCCTTGT TTAGGAGCAT TTGGTGGACC TGTCTTTCCA 
TGGCTGGTGT TAGGGGGATA CGATGGTGAT AACTATAATA ATGCTACTGC TCTTGTTATA 
ACTCTTCCTG TTAATAACTA CTACAATGAC TCCAAGAAGA TAATGAAAGC CCTGGCGTGG 
GAAAAGGAGT TTATTAACTT TTTGAAGAAC TATGATAATC CAAATTTGAC TATATCATTT 
TCTGCGGAAC GGAGCATTGA AGACGAAATC AATCGTGAAA GCAGGAGTGA TGTCAGCACT 
GTTGTAATAA GCTATGTTGT AATGTTTGTG TATATCTCCA TAGCTTTGGG ACATATCCAG 
AGCTGGGGAA GGCTGCTGGT GGATTCAAAG ATCTCCCTGG GCATTGCAGG CATCCTGATC 
GTCCTGAGCT CAGTGGCATG TTCCATAGGC ATTTTCAGTT ACTTTGGAGT TCCAGTGACA 
CTGATTGTGG CAGAAGTTAT TCCCTTCTTG GTGCTGGCTA TTGGGGTGGA CAACTTATTT 
ATTATAGTCC AGACACTTCA GAGAGATGAG CGTCTTGAGG GTGAAACTCT TGATAAGCAG 
ATTGGCAGAG TCCTGGGAGA TGTGGCACCT AGCATGTTTC TCTCATCTTT TTCTGAGACC 
GTGGCATTCT TCTTAGGGAC ACTGTCTACC ATGCCAGCAG TCCGCACGTT CTCCCTGTTT 
GCTGGAATGG CTGTGCTCAT AGATTTTATC CTTCAAGTCA CCTGCTTTGT AAGTCTCCTG 
GGCTTGGATA TTAGGCGCCA AGAGAGGAAT AGACTAGATA TTCTGTGTTG CATCAAAGGC 
AGTGAAGAAA TGAGGGGAGT CCAACGCTCC GAGAGCATCT TATTTCTGTT CTTCAAAAAC 
CTCTATTCTC CATATTTGCT TAAGGATTGG ATGAGACCAA TAGTGATTGC CTTGTTTGTG 
GGTCTTCTGT CATTCAGTAC AGCAGTGATA CACAATGTAG AGATTGGACT CGATCAGTCT 
CTTTCAATGC CAGATGATTC CTATGTAATG AATTACTTCA AACAGTTGGG CAAATACATG 
CATGCAGGTC CTCCTGTCTA CTTTGTTCTA GAAGAAGGGC ACAACTACAC CTCTCTGGAA 
GGGCAAAACA TGGTGTGTGG TGGAACGGGA TGTAATAATG ATTCACTTGT CCAGCAAGTC 
TTCAATGCTG CAGAAATTAG TAGCTACACA AGGATAGGCT ATGCCCCATC ATCCTGGATT 
GATGACTATT TTGACTGGGT GAAACCACAG TCATCCTGTT GCAGAGTCTA CAATACCACG 
GGACAGTTCT GCAATGCTTC AGTCACAGAT CCATCCTGCA CTCGTTGTCG ACCACTGAGT 
CAAGAAGGCA AGCAAAGACC ACAGGGTGAA GACTTCATGA CATTTTTGCC AATGTTCCTG 
TCTGACAACC CTAATCCTAA ATGTGGCAAA GGAGGACATG CTGCATATAG CTCAGCTGTT 
GACTTGATAA AGAACAAAAC TGATGTTGGG GCTACTTATT TTATGACGTA CCATACTGTC 
CTGAAAAAAT CATCTGACTA TATTGATGCT ATGAAGAAGG CTCGGGACAT AGCAGACAAC 
ATCACTGAAA CCATGGGCAT CAAAGAGAAG AACTATCGTG TGTTTCCTTA CAGTGTGTTT 
TATGTCTTCT ATGAACAATA TTTGACAATT GTGAATGATG CCATCTTTAA CCTCTGCATC 
TCCTTGGGAT CGATATTTTT GGTGACAGCT GTGCTGCTTG GGTTTGAAGT ATGGGCTGCT 
GTTGTGGTTA CCGTAACGAT TGCTATGATA ATTGTCAACA TGTTTGGAGT GATGTGGCTC 
TGGGGCATCA GCTTGAATGC AGTTTCATTA GTAAATCTCG TCATGAGCTG TGGCATTGCT 
GTGGAATTCT GCAGCCACGT GACGAGAGCA TTCACTGTTA GTACTAAAGG CAGTAGAGTG 
GAGCGTGCAG AAGAAGCTCT GTCTCACATG GGCAGTTCAG TCTTCAGTGG AATCACACTA 
ACCAAGTTTG GAGGAATTGT AGTACTGGCT TTTTCCAAGT CTCAGATCTT CAAGATATTC 
TACTTCAGGA TGTATCTAGC TATGGTTCTG CTGGGAGCAA CGCATGGACT AATATTTCTT 
CCAGTTCTGC TTAGTTACAT AGGTCCATCA GTAAATAAAG CTAAAACTCG TGCTGCGCAG 
GAGAGGTCTA GAGGCACAGA ACGAGAGAGA CTCCTCTACT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004939841.1
CDS	57..3881
Translation	MELVSGLEQSTGRDSVLPQQCVWYGECGVASGDKRYNCAYDGPPIALPKGGYDLMQELCPGLFFGNVSTCCDILQLQTLKNNLQLPLQFLSRCPSCFYNLINLFCELTCSPNQSEFLNVTSTIPYYDPVSKENKSSITELQYFIGERFANEMYNACKDVEAPSSNVKALGLLCGKDVKDCNATNWIEYMFNKDNGQTPFSIIPIFSDAPVHGMNPMNNATKGCNESVDDSTGPCSCQDCSIVCGPKPQPPPLPAPWLLFGLDAVYIIMWISYMGFLLIFFALVFGVWCYRRRHFVSEYTPIDSNVAFSANSHRDNGKITCGERLGERFENGLRMTFTSWGAFCVRNPRPVILFSVVFIAMCCSGFVYIKATTNPVDLWSAPSSQARKEKEYFDKHFGPFFRTEQLIIQAPNSHPDIYSPYPSGADVPFGPPLNKDILHQVLDLQDAIVNIAASFDNETVTLKDICLAPLAPYNNNCTILSVLNYFQNSHSVLDHSVGDDFFVYADYHTHFLYCVRAPASLNDTSLLHDPCLGAFGGPVFPWLVLGGYDGDNYNNATALVITLPVNNYYNDSKKIMKALAWEKEFINFLKNYDNPNLTISFSAERSIEDEINRESRSDVSTVVISYVVMFVYISIALGHIQSWGRLLVDSKISLGIAGILIVLSSVACSIGIFSYFGVPVTLIVAEVIPFLVLAIGVDNLFIIVQTLQRDERLEGETLDKQIGRVLGDVAPSMFLSSFSETVAFFLGTLSTMPAVRTFSLFAGMAVLIDFILQVTCFVSLLGLDIRRQERNRLDILCCIKGSEEMRGVQRSESILFLFFKNLYSPYLLKDWMRPIVIALFVGLLSFSTAVIHNVEIGLDQSLSMPDDSYVMNYFKQLGKYMHAGPPVYFVLEEGHNYTSLEGQNMVCGGTGCNNDSLVQQVFNAAEISSYTRIGYAPSSWIDDYFDWVKPQSSCCRVYNTTGQFCNASVTDPSCTRCRPLSQEGKQRPQGEDFMTFLPMFLSDNPNPKCGKGGHAAYSSAVDLIKNKTDVGATYFMTYHTVLKKSSDYIDAMKKARDIADNITETMGIKEKNYRVFPYSVFYVFYEQYLTIVNDAIFNLCISLGSIFLVTAVLLGFEVWAAVVVTVTIAMIIVNMFGVMWLWGISLNAVSLVNLVMSCGIAVEFCSHVTRAFTVSTKGSRVERAEEALSHMGSSVFSGITLTKFGGIVVLAFSKSQIFKIFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAKTRAAQERSRGTERERLLYF

Target ORF information:

RefSeq Version	XM_004939784.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Niemann-Pick disease, type C1 (NPC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004939784.2

ORF Insert Sequence:

ATGGAGCTTG TCTCAGGACT TGAACAGAGT ACTGGAAGAG ACAGCGTACT TCCACAACAG 
TGTGTCTGGT ACGGAGAATG TGGAGTTGCA TCTGGAGACA AGAGATACAA CTGTGCTTAT 
GATGGACCAC CTATAGCTCT ACCAAAGGGT GGCTATGACT TGATGCAGGA GCTCTGTCCA 
GGTTTATTCT TTGGCAATGT CAGTACTTGC TGTGATATCC TTCAGCTTCA GACTTTGAAA 
AACAACTTGC AGCTGCCTCT GCAGTTTCTT TCCAGGTGTC CATCATGTTT CTACAACTTG 
ATTAACCTCT TCTGTGAACT GACTTGCAGT CCAAATCAGT CTGAGTTTTT GAATGTTACA 
AGCACCATAC CTTATTATGA TCCAGTTTCG AAAGAAAACA AAAGCAGTAT TACAGAGCTG 
CAGTACTTCA TTGGAGAACG CTTTGCAAAT GAAATGTATA ATGCCTGCAA AGATGTTGAG 
GCTCCATCAA GTAATGTTAA GGCATTGGGG CTGCTGTGTG GGAAGGACGT CAAGGACTGC 
AATGCTACCA ACTGGATTGA GTACATGTTT AATAAGGATA ACGGACAAAC TCCTTTCAGC 
ATTATTCCTA TCTTTTCAGA TGCTCCTGTC CATGGAATGA ATCCTATGAA CAATGCTACC 
AAAGGCTGTA ATGAGTCTGT GGATGATTCA ACAGGACCAT GCAGCTGTCA AGACTGTTCA 
ATTGTTTGTG GTCCAAAACC TCAGCCCCCT CCATTACCTG CTCCTTGGCT TTTATTTGGT 
TTGGATGCTG TGTATATCAT CATGTGGATC TCTTACATGG GGTTTCTACT CATATTTTTT 
GCTCTAGTTT TTGGAGTCTG GTGTTACAGG AGGCGGCATT TTGTCTCTGA GTATACACCA 
ATTGACAGTA ATGTAGCCTT TTCTGCGAAC TCTCATCGTG ATAATGGGAA AATTACCTGT 
GGTGAGAGGC TTGGTGAAAG GTTTGAGAAT GGCCTAAGGA TGACATTTAC ATCATGGGGT 
GCTTTCTGTG TCAGAAATCC GCGCCCGGTT ATCCTCTTCT CTGTGGTCTT TATTGCTATG 
TGCTGTTCAG GCTTTGTATA TATTAAAGCA ACTACAAACC CTGTAGATCT CTGGTCAGCT 
CCAAGCAGCC AAGCCCGCAA GGAGAAGGAG TACTTTGACA AACACTTTGG GCCTTTCTTT 
CGTACTGAGC AACTTATTAT TCAAGCGCCA AACAGCCATC CAGACATCTA TTCACCTTAC 
CCATCAGGAG CAGATGTGCC TTTTGGACCT CCACTCAACA AAGATATTCT CCATCAGGTG 
CTTGATTTGC AAGATGCTAT TGTGAACATA GCTGCGTCTT TTGACAATGA AACTGTTACG 
CTGAAAGACA TTTGCTTGGC TCCTCTTGCT CCCTACAACA ACAACTGCAC TATATTGAGT 
GTACTCAACT ACTTCCAAAA CAGTCATTCT GTTCTAGATC ACAGTGTGGG GGATGACTTC 
TTTGTCTATG CTGACTATCA CACTCACTTC TTATATTGTG TTCGGGCTCC AGCATCACTG 
AATGACACAA GTTTGCTTCA CGATCCTTGT TTAGGAGCAT TTGGTGGACC TGTCTTTCCA 
TGGCTGGTGT TAGGGGGATA CGATGGTGAT AACTATAATA ATGCTACTGC TCTTGTTATA 
ACTCTTCCTG TTAATAACTA CTACAATGAC TCCAAGAAGA TAATGAAAGC CCTGGCGTGG 
GAAAAGGAGT TTATTAACTT TTTGAAGAAC TATGATAATC CAAATTTGAC TATATCATTT 
TCTGCGGAAC GGAGCATTGA AGACGAAATC AATCGTGAAA GCAGGAGTGA TGTCAGCACT 
GTTGTAATAA GCTATGTTGT AATGTTTGTG TATATCTCCA TAGCTTTGGG ACATATCCAG 
AGCTGGGGAA GGCTGCTGGT GGATTCAAAG ATCTCCCTGG GCATTGCAGG CATCCTGATC 
GTCCTGAGCT CAGTGGCATG TTCCATAGGC ATTTTCAGTT ACTTTGGAGT TCCAGTGACA 
CTGATTGTGG CAGAAGTTAT TCCCTTCTTG GTGCTGGCTA TTGGGGTGGA CAACTTATTT 
ATTATAGTCC AGACACTTCA GAGAGATGAG CGTCTTGAGG GTGAAACTCT TGATAAGCAG 
ATTGGCAGAG TCCTGGGAGA TGTGGCACCT AGCATGTTTC TCTCATCTTT TTCTGAGACC 
GTGGCATTCT TCTTAGGGAC ACTGTCTACC ATGCCAGCAG TCCGCACGTT CTCCCTGTTT 
GCTGGAATGG CTGTGCTCAT AGATTTTATC CTTCAAGTCA CCTGCTTTGT AAGTCTCCTG 
GGCTTGGATA TTAGGCGCCA AGAGAGGAAT AGACTAGATA TTCTGTGTTG CATCAAAGGC 
AGTGAAGAAA TGAGGGGAGT CCAACGCTCC GAGAGCATCT TATTTCTGTT CTTCAAAAAC 
CTCTATTCTC CATATTTGCT TAAGGATTGG ATGAGACCAA TAGTGATTGC CTTGTTTGTG 
GGTCTTCTGT CATTCAGTAC AGCAGTGATA CACAATGTAG AGATTGGACT CGATCAGTCT 
CTTTCAATGC CAGATGATTC CTATGTAATG AATTACTTCA AACAGTTGGG CAAATACATG 
CATGCAGGTC CTCCTGTCTA CTTTGTTCTA GAAGAAGGGC ACAACTACAC CTCTCTGGAA 
GGGCAAAACA TGGTGTGTGG TGGAACGGGA TGTAATAATG ATTCACTTGT CCAGCAAGTC 
TTCAATGCTG CAGAAATTAG TAGCTACACA AGGATAGGCT ATGCCCCATC ATCCTGGATT 
GATGACTATT TTGACTGGGT GAAACCACAG TCATCCTGTT GCAGAGTCTA CAATACCACG 
GGACAGTTCT GCAATGCTTC AGTCACAGAT CCATCCTGCA CTCGTTGTCG ACCACTGAGT 
CAAGAAGGCA AGCAAAGACC ACAGGGTGAA GACTTCATGA CATTTTTGCC AATGTTCCTG 
TCTGACAACC CTAATCCTAA ATGTGGCAAA GGAGGACATG CTGCATATAG CTCAGCTGTT 
GACTTGATAA AGAACAAAAC TGATGTTGGG GCTACTTATT TTATGACGTA CCATACTGTC 
CTGAAAAAAT CATCTGACTA TATTGATGCT ATGAAGAAGG CTCGGGACAT AGCAGACAAC 
ATCACTGAAA CCATGGGCAT CAAAGAGAAG AACTATCGTG TGTTTCCTTA CAGTGTGTTT 
TATGTCTTCT ATGAACAATA TTTGACAATT GTGAATGATG CCATCTTTAA CCTCTGCATC 
TCCTTGGGAT CGATATTTTT GGTGACAGCT GTGCTGCTTG GGTTTGAAGT ATGGGCTGCT 
GTTGTGGTTA CCGTAACGAT TGCTATGATA ATTGTCAACA TGTTTGGAGT GATGTGGCTC 
TGGGGCATCA GCTTGAATGC AGTTTCATTA GTAAATCTCG TCATGAGCTG TGGCATTGCT 
GTGGAATTCT GCAGCCACGT GACGAGAGCA TTCACTGTTA GTACTAAAGG CAGTAGAGTG 
GAGCGTGCAG AAGAAGCTCT GTCTCACATG GGCAGTTCAG TCTTCAGTGG AATCACACTA 
ACCAAGTTTG GAGGAATTGT AGTACTGGCT TTTTCCAAGT CTCAGATCTT CAAGATATTC 
TACTTCAGGA TGTATCTAGC TATGGTTCTG CTGGGAGCAA CGCATGGACT AATATTTCTT 
CCAGTTCTGC TTAGTTACAT AGGTCCATCA GTAAATAAAG CTAAAACTCG TGCTGCGCAG 
GAGAGGTCTA GAGGCACAGA ACGAGAGAGA CTCCTCTACT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPC1 ( XM_004939784.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004939841.1 Gallus gallus NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X2, mRNA.

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CloneID	OGa05147
Clone ID Related Accession (Same CDS sequence)	XM_004939784.2 , XM_004939784.3
Accession Version	XM_004939784.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	NPC intracellular cholesterol transporter 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004939784.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTTG TCTCAGGACT TGAACAGAGT ACTGGAAGAG ACAGCGTACT TCCACAACAG 
TGTGTCTGGT ACGGAGAATG TGGAGTTGCA TCTGGAGACA AGAGATACAA CTGTGCTTAT 
GATGGACCAC CTATAGCTCT ACCAAAGGGT GGCTATGACT TGATGCAGGA GCTCTGTCCA 
GGTTTATTCT TTGGCAATGT CAGTACTTGC TGTGATATCC TTCAGCTTCA GACTTTGAAA 
AACAACTTGC AGCTGCCTCT GCAGTTTCTT TCCAGGTGTC CATCATGTTT CTACAACTTG 
ATTAACCTCT TCTGTGAACT GACTTGCAGT CCAAATCAGT CTGAGTTTTT GAATGTTACA 
AGCACCATAC CTTATTATGA TCCAGTTTCG AAAGAAAACA AAAGCAGTAT TACAGAGCTG 
CAGTACTTCA TTGGAGAACG CTTTGCAAAT GAAATGTATA ATGCCTGCAA AGATGTTGAG 
GCTCCATCAA GTAATGTTAA GGCATTGGGG CTGCTGTGTG GGAAGGACGT CAAGGACTGC 
AATGCTACCA ACTGGATTGA GTACATGTTT AATAAGGATA ACGGACAAAC TCCTTTCAGC 
ATTATTCCTA TCTTTTCAGA TGCTCCTGTC CATGGAATGA ATCCTATGAA CAATGCTACC 
AAAGGCTGTA ATGAGTCTGT GGATGATTCA ACAGGACCAT GCAGCTGTCA AGACTGTTCA 
ATTGTTTGTG GTCCAAAACC TCAGCCCCCT CCATTACCTG CTCCTTGGCT TTTATTTGGT 
TTGGATGCTG TGTATATCAT CATGTGGATC TCTTACATGG GGTTTCTACT CATATTTTTT 
GCTCTAGTTT TTGGAGTCTG GTGTTACAGG AGGCGGCATT TTGTCTCTGA GTATACACCA 
ATTGACAGTA ATGTAGCCTT TTCTGCGAAC TCTCATCGTG ATAATGGGAA AATTACCTGT 
GGTGAGAGGC TTGGTGAAAG GTTTGAGAAT GGCCTAAGGA TGACATTTAC ATCATGGGGT 
GCTTTCTGTG TCAGAAATCC GCGCCCGGTT ATCCTCTTCT CTGTGGTCTT TATTGCTATG 
TGCTGTTCAG GCTTTGTATA TATTAAAGCA ACTACAAACC CTGTAGATCT CTGGTCAGCT 
CCAAGCAGCC AAGCCCGCAA GGAGAAGGAG TACTTTGACA AACACTTTGG GCCTTTCTTT 
CGTACTGAGC AACTTATTAT TCAAGCGCCA AACAGCCATC CAGACATCTA TTCACCTTAC 
CCATCAGGAG CAGATGTGCC TTTTGGACCT CCACTCAACA AAGATATTCT CCATCAGGTG 
CTTGATTTGC AAGATGCTAT TGTGAACATA GCTGCGTCTT TTGACAATGA AACTGTTACG 
CTGAAAGACA TTTGCTTGGC TCCTCTTGCT CCCTACAACA ACAACTGCAC TATATTGAGT 
GTACTCAACT ACTTCCAAAA CAGTCATTCT GTTCTAGATC ACAGTGTGGG GGATGACTTC 
TTTGTCTATG CTGACTATCA CACTCACTTC TTATATTGTG TTCGGGCTCC AGCATCACTG 
AATGACACAA GTTTGCTTCA CGATCCTTGT TTAGGAGCAT TTGGTGGACC TGTCTTTCCA 
TGGCTGGTGT TAGGGGGATA CGATGGTGAT AACTATAATA ATGCTACTGC TCTTGTTATA 
ACTCTTCCTG TTAATAACTA CTACAATGAC TCCAAGAAGA TAATGAAAGC CCTGGCGTGG 
GAAAAGGAGT TTATTAACTT TTTGAAGAAC TATGATAATC CAAATTTGAC TATATCATTT 
TCTGCGGAAC GGAGCATTGA AGACGAAATC AATCGTGAAA GCAGGAGTGA TGTCAGCACT 
GTTGTAATAA GCTATGTTGT AATGTTTGTG TATATCTCCA TAGCTTTGGG ACATATCCAG 
AGCTGGGGAA GGCTGCTGGT GGATTCAAAG ATCTCCCTGG GCATTGCAGG CATCCTGATC 
GTCCTGAGCT CAGTGGCATG TTCCATAGGC ATTTTCAGTT ACTTTGGAGT TCCAGTGACA 
CTGATTGTGG CAGAAGTTAT TCCCTTCTTG GTGCTGGCTA TTGGGGTGGA CAACTTATTT 
ATTATAGTCC AGACACTTCA GAGAGATGAG CGTCTTGAGG GTGAAACTCT TGATAAGCAG 
ATTGGCAGAG TCCTGGGAGA TGTGGCACCT AGCATGTTTC TCTCATCTTT TTCTGAGACC 
GTGGCATTCT TCTTAGGGAC ACTGTCTACC ATGCCAGCAG TCCGCACGTT CTCCCTGTTT 
GCTGGAATGG CTGTGCTCAT AGATTTTATC CTTCAAGTCA CCTGCTTTGT AAGTCTCCTG 
GGCTTGGATA TTAGGCGCCA AGAGAGGAAT AGACTAGATA TTCTGTGTTG CATCAAAGGC 
AGTGAAGAAA TGAGGGGAGT CCAACGCTCC GAGAGCATCT TATTTCTGTT CTTCAAAAAC 
CTCTATTCTC CATATTTGCT TAAGGATTGG ATGAGACCAA TAGTGATTGC CTTGTTTGTG 
GGTCTTCTGT CATTCAGTAC AGCAGTGATA CACAATGTAG AGATTGGACT CGATCAGTCT 
CTTTCAATGC CAGATGATTC CTATGTAATG AATTACTTCA AACAGTTGGG CAAATACATG 
CATGCAGGTC CTCCTGTCTA CTTTGTTCTA GAAGAAGGGC ACAACTACAC CTCTCTGGAA 
GGGCAAAACA TGGTGTGTGG TGGAACGGGA TGTAATAATG ATTCACTTGT CCAGCAAGTC 
TTCAATGCTG CAGAAATTAG TAGCTACACA AGGATAGGCT ATGCCCCATC ATCCTGGATT 
GATGACTATT TTGACTGGGT GAAACCACAG TCATCCTGTT GCAGAGTCTA CAATACCACG 
GGACAGTTCT GCAATGCTTC AGTCACAGAT CCATCCTGCA CTCGTTGTCG ACCACTGAGT 
CAAGAAGGCA AGCAAAGACC ACAGGGTGAA GACTTCATGA CATTTTTGCC AATGTTCCTG 
TCTGACAACC CTAATCCTAA ATGTGGCAAA GGAGGACATG CTGCATATAG CTCAGCTGTT 
GACTTGATAA AGAACAAAAC TGATGTTGGG GCTACTTATT TTATGACGTA CCATACTGTC 
CTGAAAAAAT CATCTGACTA TATTGATGCT ATGAAGAAGG CTCGGGACAT AGCAGACAAC 
ATCACTGAAA CCATGGGCAT CAAAGAGAAG AACTATCGTG TGTTTCCTTA CAGTGTGTTT 
TATGTCTTCT ATGAACAATA TTTGACAATT GTGAATGATG CCATCTTTAA CCTCTGCATC 
TCCTTGGGAT CGATATTTTT GGTGACAGCT GTGCTGCTTG GGTTTGAAGT ATGGGCTGCT 
GTTGTGGTTA CCGTAACGAT TGCTATGATA ATTGTCAACA TGTTTGGAGT GATGTGGCTC 
TGGGGCATCA GCTTGAATGC AGTTTCATTA GTAAATCTCG TCATGAGCTG TGGCATTGCT 
GTGGAATTCT GCAGCCACGT GACGAGAGCA TTCACTGTTA GTACTAAAGG CAGTAGAGTG 
GAGCGTGCAG AAGAAGCTCT GTCTCACATG GGCAGTTCAG TCTTCAGTGG AATCACACTA 
ACCAAGTTTG GAGGAATTGT AGTACTGGCT TTTTCCAAGT CTCAGATCTT CAAGATATTC 
TACTTCAGGA TGTATCTAGC TATGGTTCTG CTGGGAGCAA CGCATGGACT AATATTTCTT 
CCAGTTCTGC TTAGTTACAT AGGTCCATCA GTAAATAAAG CTAAAACTCG TGCTGCGCAG 
GAGAGGTCTA GAGGCACAGA ACGAGAGAGA CTCCTCTACT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004939841.1
CDS	74..3898
Translation	MELVSGLEQSTGRDSVLPQQCVWYGECGVASGDKRYNCAYDGPPIALPKGGYDLMQELCPGLFFGNVSTCCDILQLQTLKNNLQLPLQFLSRCPSCFYNLINLFCELTCSPNQSEFLNVTSTIPYYDPVSKENKSSITELQYFIGERFANEMYNACKDVEAPSSNVKALGLLCGKDVKDCNATNWIEYMFNKDNGQTPFSIIPIFSDAPVHGMNPMNNATKGCNESVDDSTGPCSCQDCSIVCGPKPQPPPLPAPWLLFGLDAVYIIMWISYMGFLLIFFALVFGVWCYRRRHFVSEYTPIDSNVAFSANSHRDNGKITCGERLGERFENGLRMTFTSWGAFCVRNPRPVILFSVVFIAMCCSGFVYIKATTNPVDLWSAPSSQARKEKEYFDKHFGPFFRTEQLIIQAPNSHPDIYSPYPSGADVPFGPPLNKDILHQVLDLQDAIVNIAASFDNETVTLKDICLAPLAPYNNNCTILSVLNYFQNSHSVLDHSVGDDFFVYADYHTHFLYCVRAPASLNDTSLLHDPCLGAFGGPVFPWLVLGGYDGDNYNNATALVITLPVNNYYNDSKKIMKALAWEKEFINFLKNYDNPNLTISFSAERSIEDEINRESRSDVSTVVISYVVMFVYISIALGHIQSWGRLLVDSKISLGIAGILIVLSSVACSIGIFSYFGVPVTLIVAEVIPFLVLAIGVDNLFIIVQTLQRDERLEGETLDKQIGRVLGDVAPSMFLSSFSETVAFFLGTLSTMPAVRTFSLFAGMAVLIDFILQVTCFVSLLGLDIRRQERNRLDILCCIKGSEEMRGVQRSESILFLFFKNLYSPYLLKDWMRPIVIALFVGLLSFSTAVIHNVEIGLDQSLSMPDDSYVMNYFKQLGKYMHAGPPVYFVLEEGHNYTSLEGQNMVCGGTGCNNDSLVQQVFNAAEISSYTRIGYAPSSWIDDYFDWVKPQSSCCRVYNTTGQFCNASVTDPSCTRCRPLSQEGKQRPQGEDFMTFLPMFLSDNPNPKCGKGGHAAYSSAVDLIKNKTDVGATYFMTYHTVLKKSSDYIDAMKKARDIADNITETMGIKEKNYRVFPYSVFYVFYEQYLTIVNDAIFNLCISLGSIFLVTAVLLGFEVWAAVVVTVTIAMIIVNMFGVMWLWGISLNAVSLVNLVMSCGIAVEFCSHVTRAFTVSTKGSRVERAEEALSHMGSSVFSGITLTKFGGIVVLAFSKSQIFKIFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAKTRAAQERSRGTERERLLYF

Target ORF information:

RefSeq Version	XM_004939784.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004939784.3

ORF Insert Sequence:

ATGGAGCTTG TCTCAGGACT TGAACAGAGT ACTGGAAGAG ACAGCGTACT TCCACAACAG 
TGTGTCTGGT ACGGAGAATG TGGAGTTGCA TCTGGAGACA AGAGATACAA CTGTGCTTAT 
GATGGACCAC CTATAGCTCT ACCAAAGGGT GGCTATGACT TGATGCAGGA GCTCTGTCCA 
GGTTTATTCT TTGGCAATGT CAGTACTTGC TGTGATATCC TTCAGCTTCA GACTTTGAAA 
AACAACTTGC AGCTGCCTCT GCAGTTTCTT TCCAGGTGTC CATCATGTTT CTACAACTTG 
ATTAACCTCT TCTGTGAACT GACTTGCAGT CCAAATCAGT CTGAGTTTTT GAATGTTACA 
AGCACCATAC CTTATTATGA TCCAGTTTCG AAAGAAAACA AAAGCAGTAT TACAGAGCTG 
CAGTACTTCA TTGGAGAACG CTTTGCAAAT GAAATGTATA ATGCCTGCAA AGATGTTGAG 
GCTCCATCAA GTAATGTTAA GGCATTGGGG CTGCTGTGTG GGAAGGACGT CAAGGACTGC 
AATGCTACCA ACTGGATTGA GTACATGTTT AATAAGGATA ACGGACAAAC TCCTTTCAGC 
ATTATTCCTA TCTTTTCAGA TGCTCCTGTC CATGGAATGA ATCCTATGAA CAATGCTACC 
AAAGGCTGTA ATGAGTCTGT GGATGATTCA ACAGGACCAT GCAGCTGTCA AGACTGTTCA 
ATTGTTTGTG GTCCAAAACC TCAGCCCCCT CCATTACCTG CTCCTTGGCT TTTATTTGGT 
TTGGATGCTG TGTATATCAT CATGTGGATC TCTTACATGG GGTTTCTACT CATATTTTTT 
GCTCTAGTTT TTGGAGTCTG GTGTTACAGG AGGCGGCATT TTGTCTCTGA GTATACACCA 
ATTGACAGTA ATGTAGCCTT TTCTGCGAAC TCTCATCGTG ATAATGGGAA AATTACCTGT 
GGTGAGAGGC TTGGTGAAAG GTTTGAGAAT GGCCTAAGGA TGACATTTAC ATCATGGGGT 
GCTTTCTGTG TCAGAAATCC GCGCCCGGTT ATCCTCTTCT CTGTGGTCTT TATTGCTATG 
TGCTGTTCAG GCTTTGTATA TATTAAAGCA ACTACAAACC CTGTAGATCT CTGGTCAGCT 
CCAAGCAGCC AAGCCCGCAA GGAGAAGGAG TACTTTGACA AACACTTTGG GCCTTTCTTT 
CGTACTGAGC AACTTATTAT TCAAGCGCCA AACAGCCATC CAGACATCTA TTCACCTTAC 
CCATCAGGAG CAGATGTGCC TTTTGGACCT CCACTCAACA AAGATATTCT CCATCAGGTG 
CTTGATTTGC AAGATGCTAT TGTGAACATA GCTGCGTCTT TTGACAATGA AACTGTTACG 
CTGAAAGACA TTTGCTTGGC TCCTCTTGCT CCCTACAACA ACAACTGCAC TATATTGAGT 
GTACTCAACT ACTTCCAAAA CAGTCATTCT GTTCTAGATC ACAGTGTGGG GGATGACTTC 
TTTGTCTATG CTGACTATCA CACTCACTTC TTATATTGTG TTCGGGCTCC AGCATCACTG 
AATGACACAA GTTTGCTTCA CGATCCTTGT TTAGGAGCAT TTGGTGGACC TGTCTTTCCA 
TGGCTGGTGT TAGGGGGATA CGATGGTGAT AACTATAATA ATGCTACTGC TCTTGTTATA 
ACTCTTCCTG TTAATAACTA CTACAATGAC TCCAAGAAGA TAATGAAAGC CCTGGCGTGG 
GAAAAGGAGT TTATTAACTT TTTGAAGAAC TATGATAATC CAAATTTGAC TATATCATTT 
TCTGCGGAAC GGAGCATTGA AGACGAAATC AATCGTGAAA GCAGGAGTGA TGTCAGCACT 
GTTGTAATAA GCTATGTTGT AATGTTTGTG TATATCTCCA TAGCTTTGGG ACATATCCAG 
AGCTGGGGAA GGCTGCTGGT GGATTCAAAG ATCTCCCTGG GCATTGCAGG CATCCTGATC 
GTCCTGAGCT CAGTGGCATG TTCCATAGGC ATTTTCAGTT ACTTTGGAGT TCCAGTGACA 
CTGATTGTGG CAGAAGTTAT TCCCTTCTTG GTGCTGGCTA TTGGGGTGGA CAACTTATTT 
ATTATAGTCC AGACACTTCA GAGAGATGAG CGTCTTGAGG GTGAAACTCT TGATAAGCAG 
ATTGGCAGAG TCCTGGGAGA TGTGGCACCT AGCATGTTTC TCTCATCTTT TTCTGAGACC 
GTGGCATTCT TCTTAGGGAC ACTGTCTACC ATGCCAGCAG TCCGCACGTT CTCCCTGTTT 
GCTGGAATGG CTGTGCTCAT AGATTTTATC CTTCAAGTCA CCTGCTTTGT AAGTCTCCTG 
GGCTTGGATA TTAGGCGCCA AGAGAGGAAT AGACTAGATA TTCTGTGTTG CATCAAAGGC 
AGTGAAGAAA TGAGGGGAGT CCAACGCTCC GAGAGCATCT TATTTCTGTT CTTCAAAAAC 
CTCTATTCTC CATATTTGCT TAAGGATTGG ATGAGACCAA TAGTGATTGC CTTGTTTGTG 
GGTCTTCTGT CATTCAGTAC AGCAGTGATA CACAATGTAG AGATTGGACT CGATCAGTCT 
CTTTCAATGC CAGATGATTC CTATGTAATG AATTACTTCA AACAGTTGGG CAAATACATG 
CATGCAGGTC CTCCTGTCTA CTTTGTTCTA GAAGAAGGGC ACAACTACAC CTCTCTGGAA 
GGGCAAAACA TGGTGTGTGG TGGAACGGGA TGTAATAATG ATTCACTTGT CCAGCAAGTC 
TTCAATGCTG CAGAAATTAG TAGCTACACA AGGATAGGCT ATGCCCCATC ATCCTGGATT 
GATGACTATT TTGACTGGGT GAAACCACAG TCATCCTGTT GCAGAGTCTA CAATACCACG 
GGACAGTTCT GCAATGCTTC AGTCACAGAT CCATCCTGCA CTCGTTGTCG ACCACTGAGT 
CAAGAAGGCA AGCAAAGACC ACAGGGTGAA GACTTCATGA CATTTTTGCC AATGTTCCTG 
TCTGACAACC CTAATCCTAA ATGTGGCAAA GGAGGACATG CTGCATATAG CTCAGCTGTT 
GACTTGATAA AGAACAAAAC TGATGTTGGG GCTACTTATT TTATGACGTA CCATACTGTC 
CTGAAAAAAT CATCTGACTA TATTGATGCT ATGAAGAAGG CTCGGGACAT AGCAGACAAC 
ATCACTGAAA CCATGGGCAT CAAAGAGAAG AACTATCGTG TGTTTCCTTA CAGTGTGTTT 
TATGTCTTCT ATGAACAATA TTTGACAATT GTGAATGATG CCATCTTTAA CCTCTGCATC 
TCCTTGGGAT CGATATTTTT GGTGACAGCT GTGCTGCTTG GGTTTGAAGT ATGGGCTGCT 
GTTGTGGTTA CCGTAACGAT TGCTATGATA ATTGTCAACA TGTTTGGAGT GATGTGGCTC 
TGGGGCATCA GCTTGAATGC AGTTTCATTA GTAAATCTCG TCATGAGCTG TGGCATTGCT 
GTGGAATTCT GCAGCCACGT GACGAGAGCA TTCACTGTTA GTACTAAAGG CAGTAGAGTG 
GAGCGTGCAG AAGAAGCTCT GTCTCACATG GGCAGTTCAG TCTTCAGTGG AATCACACTA 
ACCAAGTTTG GAGGAATTGT AGTACTGGCT TTTTCCAAGT CTCAGATCTT CAAGATATTC 
TACTTCAGGA TGTATCTAGC TATGGTTCTG CTGGGAGCAA CGCATGGACT AATATTTCTT 
CCAGTTCTGC TTAGTTACAT AGGTCCATCA GTAAATAAAG CTAAAACTCG TGCTGCGCAG 
GAGAGGTCTA GAGGCACAGA ACGAGAGAGA CTCCTCTACT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPC1 ( XM_419162.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_419162.3 Gallus gallus Niemann-Pick disease, type C1 (NPC1), transcript variant X3, mRNA.

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CloneID	OGa05149
Clone ID Related Accession (Same CDS sequence)	XM_419162.5 , XM_419162.6
Accession Version	XM_419162.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	Niemann-Pick C1 protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_419162.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGGCC CGCAAGGCAG CCCCGGGCCG GGCACCCGCC TCCTGCTCCT CTTTCTCCTG 
CTGCTGCTGT CCCCGGCACA GGTACTTCCA CAACAGTGTG TCTGGTACGG AGAATGTGGA 
GTTGCATCTG GAGACAAGAG ATACAACTGT GCTTATGATG GACCACCTAT AGCTCTACCA 
AAGGGTGGCT ATGACTTGAT GCAGGAGCTC TGTCCAGGTT TATTCTTTGG CAATGTCAGT 
ACTTGCTGTG ATATCCTTCA GCTTCAGACT TTGAAAAACA ACTTGCAGCT GCCTCTGCAG 
TTTCTTTCCA GGTGTCCATC ATGTTTCTAC AACTTGATTA ACCTCTTCTG TGAACTGACT 
TGCAGTCCAA ATCAGTCTGA GTTTTTGAAT GTTACAAGCA CCATACCTTA TTATGATCCA 
GTTTCGAAAG AAAACAAAAG CAGTATTACA GAGCTGCAGT ACTTCATTGG AGAACGCTTT 
GCAAATGAAA TGTATAATGC CTGCAAAGAT GTTGAGGCTC CATCAAGTAA TGTTAAGGCA 
TTGGGGCTGC TGTGTGGGAA GGACGTCAAG GACTGCAATG CTACCAACTG GATTGAGTAC 
ATGTTTAATA AGGATAACGG ACAAACTCCT TTCAGCATTA TTCCTATCTT TTCAGATGCT 
CCTGTCCATG GAATGAATCC TATGAACAAT GCTACCAAAG GCTGTAATGA GTCTGTGGAT 
GATTCAACAG GACCATGCAG CTGTCAAGAC TGTTCAATTG TTTGTGGTCC AAAACCTCAG 
CCCCCTCCAT TACCTGCTCC TTGGCTTTTA TTTGGTTTGG ATGCTGTGTA TATCATCATG 
TGGATCTCTT ACATGGGGTT TCTACTCATA TTTTTTGCTC TAGTTTTTGG AGTCTGGTGT 
TACAGGAGGC GGCATTTTGT CTCTGAGTAT ACACCAATTG ACAGTAATGT AGCCTTTTCT 
GCGAACTCTC ATCGTGATAA TGGGAAAATT ACCTGTGGTG AGAGGCTTGG TGAAAGGTTT 
GAGAATGGCC TAAGGATGAC ATTTACATCA TGGGGTGCTT TCTGTGTCAG AAATCCGCGC 
CCGGTTATCC TCTTCTCTGT GGTCTTTATT GCTATGTGCT GTTCAGGCTT TGTATATATT 
AAAGCAACTA CAAACCCTGT AGATCTCTGG TCAGCTCCAA GCAGCCAAGC CCGCAAGGAG 
AAGGAGTACT TTGACAAACA CTTTGGGCCT TTCTTTCGTA CTGAGCAACT TATTATTCAA 
GCGCCAAACA GCCATCCAGA CATCTATTCA CCTTACCCAT CAGGAGCAGA TGTGCCTTTT 
GGACCTCCAC TCAACAAAGA TATTCTCCAT CAGGTGCTTG ATTTGCAAGA TGCTATTGTG 
AACATAGCTG CGTCTTTTGA CAATGAAACT GTTACGCTGA AAGACATTTG CTTGGCTCCT 
CTTGCTCCCT ACAACAACAA CTGCACTATA TTGAGTGTAC TCAACTACTT CCAAAACAGT 
CATTCTGTTC TAGATCACAG TGTGGGGGAT GACTTCTTTG TCTATGCTGA CTATCACACT 
CACTTCTTAT ATTGTGTTCG GGCTCCAGCA TCACTGAATG ACACAAGTTT GCTTCACGAT 
CCTTGTTTAG GAGCATTTGG TGGACCTGTC TTTCCATGGC TGGTGTTAGG GGGATACGAT 
GGTGATAACT ATAATAATGC TACTGCTCTT GTTATAACTC TTCCTGTTAA TAACTACTAC 
AATGACTCCA AGAAGATAAT GAAAGCCCTG GCGTGGGAAA AGGAGTTTAT TAACTTTTTG 
AAGAACTATG ATAATCCAAA TTTGACTATA TCATTTTCTG CGGAACGGAG CATTGAAGAC 
GAAATCAATC GTGAAAGCAG GAGTGATGTC AGCACTGTTG TAATAAGCTA TGTTGTAATG 
TTTGTGTATA TCTCCATAGC TTTGGGACAT ATCCAGAGCT GGGGAAGGCT GCTGGTGGAT 
TCAAAGATCT CCCTGGGCAT TGCAGGCATC CTGATCGTCC TGAGCTCAGT GGCATGTTCC 
ATAGGCATTT TCAGTTACTT TGGAGTTCCA GTGACACTGA TTGTGGCAGA AGTTATTCCC 
TTCTTGGTGC TGGCTATTGG GGTGGACAAC TTATTTATTA TAGTCCAGAC ACTTCAGAGA 
GATGAGCGTC TTGAGGGTGA AACTCTTGAT AAGCAGATTG GCAGAGTCCT GGGAGATGTG 
GCACCTAGCA TGTTTCTCTC ATCTTTTTCT GAGACCGTGG CATTCTTCTT AGGGACACTG 
TCTACCATGC CAGCAGTCCG CACGTTCTCC CTGTTTGCTG GAATGGCTGT GCTCATAGAT 
TTTATCCTTC AAGTCACCTG CTTTGTAAGT CTCCTGGGCT TGGATATTAG GCGCCAAGAG 
AGGAATAGAC TAGATATTCT GTGTTGCATC AAAGGCAGTG AAGAAATGAG GGGAGTCCAA 
CGCTCCGAGA GCATCTTATT TCTGTTCTTC AAAAACCTCT ATTCTCCATA TTTGCTTAAG 
GATTGGATGA GACCAATAGT GATTGCCTTG TTTGTGGGTC TTCTGTCATT CAGTACAGCA 
GTGATACACA ATGTAGAGAT TGGACTCGAT CAGTCTCTTT CAATGCCAGA TGATTCCTAT 
GTAATGAATT ACTTCAAACA GTTGGGCAAA TACATGCATG CAGGTCCTCC TGTCTACTTT 
GTTCTAGAAG AAGGGCACAA CTACACCTCT CTGGAAGGGC AAAACATGGT GTGTGGTGGA 
ACGGGATGTA ATAATGATTC ACTTGTCCAG CAAGTCTTCA ATGCTGCAGA AATTAGTAGC 
TACACAAGGA TAGGCTATGC CCCATCATCC TGGATTGATG ACTATTTTGA CTGGGTGAAA 
CCACAGTCAT CCTGTTGCAG AGTCTACAAT ACCACGGGAC AGTTCTGCAA TGCTTCAGTC 
ACAGATCCAT CCTGCACTCG TTGTCGACCA CTGAGTCAAG AAGGCAAGCA AAGACCACAG 
GGTGAAGACT TCATGACATT TTTGCCAATG TTCCTGTCTG ACAACCCTAA TCCTAAATGT 
GGCAAAGGAG GACATGCTGC ATATAGCTCA GCTGTTGACT TGATAAAGAA CAAAACTGAT 
GTTGGGGCTA CTTATTTTAT GACGTACCAT ACTGTCCTGA AAAAATCATC TGACTATATT 
GATGCTATGA AGAAGGCTCG GGACATAGCA GACAACATCA CTGAAACCAT GGGCATCAAA 
GAGAAGAACT ATCGTGTGTT TCCTTACAGT GTGTTTTATG TCTTCTATGA ACAATATTTG 
ACAATTGTGA ATGATGCCAT CTTTAACCTC TGCATCTCCT TGGGATCGAT ATTTTTGGTG 
ACAGCTGTGC TGCTTGGGTT TGAAGTATGG GCTGCTGTTG TGGTTACCGT AACGATTGCT 
ATGATAATTG TCAACATGTT TGGAGTGATG TGGCTCTGGG GCATCAGCTT GAATGCAGTT 
TCATTAGTAA ATCTCGTCAT GAGCTGTGGC ATTGCTGTGG AATTCTGCAG CCACGTGACG 
AGAGCATTCA CTGTTAGTAC TAAAGGCAGT AGAGTGGAGC GTGCAGAAGA AGCTCTGTCT 
CACATGGGCA GTTCAGTCTT CAGTGGAATC ACACTAACCA AGTTTGGAGG AATTGTAGTA 
CTGGCTTTTT CCAAGTCTCA GATCTTCAAG ATATTCTACT TCAGGATGTA TCTAGCTATG 
GTTCTGCTGG GAGCAACGCA TGGACTAATA TTTCTTCCAG TTCTGCTTAG TTACATAGGT 
CCATCAGTAA ATAAAGCTAA AACTCGTGCT GCGCAGGAGA GGTCTAGAGG CACAGAACGA 
GAGAGACTCC TCTACTTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_419162.3
CDS	398..4258
Translation	MGGPQGSPGPGTRLLLLFLLLLLSPAQVLPQQCVWYGECGVASGDKRYNCAYDGPPIALPKGGYDLMQELCPGLFFGNVSTCCDILQLQTLKNNLQLPLQFLSRCPSCFYNLINLFCELTCSPNQSEFLNVTSTIPYYDPVSKENKSSITELQYFIGERFANEMYNACKDVEAPSSNVKALGLLCGKDVKDCNATNWIEYMFNKDNGQTPFSIIPIFSDAPVHGMNPMNNATKGCNESVDDSTGPCSCQDCSIVCGPKPQPPPLPAPWLLFGLDAVYIIMWISYMGFLLIFFALVFGVWCYRRRHFVSEYTPIDSNVAFSANSHRDNGKITCGERLGERFENGLRMTFTSWGAFCVRNPRPVILFSVVFIAMCCSGFVYIKATTNPVDLWSAPSSQARKEKEYFDKHFGPFFRTEQLIIQAPNSHPDIYSPYPSGADVPFGPPLNKDILHQVLDLQDAIVNIAASFDNETVTLKDICLAPLAPYNNNCTILSVLNYFQNSHSVLDHSVGDDFFVYADYHTHFLYCVRAPASLNDTSLLHDPCLGAFGGPVFPWLVLGGYDGDNYNNATALVITLPVNNYYNDSKKIMKALAWEKEFINFLKNYDNPNLTISFSAERSIEDEINRESRSDVSTVVISYVVMFVYISIALGHIQSWGRLLVDSKISLGIAGILIVLSSVACSIGIFSYFGVPVTLIVAEVIPFLVLAIGVDNLFIIVQTLQRDERLEGETLDKQIGRVLGDVAPSMFLSSFSETVAFFLGTLSTMPAVRTFSLFAGMAVLIDFILQVTCFVSLLGLDIRRQERNRLDILCCIKGSEEMRGVQRSESILFLFFKNLYSPYLLKDWMRPIVIALFVGLLSFSTAVIHNVEIGLDQSLSMPDDSYVMNYFKQLGKYMHAGPPVYFVLEEGHNYTSLEGQNMVCGGTGCNNDSLVQQVFNAAEISSYTRIGYAPSSWIDDYFDWVKPQSSCCRVYNTTGQFCNASVTDPSCTRCRPLSQEGKQRPQGEDFMTFLPMFLSDNPNPKCGKGGHAAYSSAVDLIKNKTDVGATYFMTYHTVLKKSSDYIDAMKKARDIADNITETMGIKEKNYRVFPYSVFYVFYEQYLTIVNDAIFNLCISLGSIFLVTAVLLGFEVWAAVVVTVTIAMIIVNMFGVMWLWGISLNAVSLVNLVMSCGIAVEFCSHVTRAFTVSTKGSRVERAEEALSHMGSSVFSGITLTKFGGIVVLAFSKSQIFKIFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAKTRAAQERSRGTERERLLYF

Target ORF information:

RefSeq Version	XM_419162.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Niemann-Pick disease, type C1 (NPC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_419162.5

ORF Insert Sequence:

ATGGGGGGCC CGCAAGGCAG CCCCGGGCCG GGCACCCGCC TCCTGCTCCT CTTTCTCCTG 
CTGCTGCTGT CCCCGGCACA GGTACTTCCA CAACAGTGTG TCTGGTACGG AGAATGTGGA 
GTTGCATCTG GAGACAAGAG ATACAACTGT GCTTATGATG GACCACCTAT AGCTCTACCA 
AAGGGTGGCT ATGACTTGAT GCAGGAGCTC TGTCCAGGTT TATTCTTTGG CAATGTCAGT 
ACTTGCTGTG ATATCCTTCA GCTTCAGACT TTGAAAAACA ACTTGCAGCT GCCTCTGCAG 
TTTCTTTCCA GGTGTCCATC ATGTTTCTAC AACTTGATTA ACCTCTTCTG TGAACTGACT 
TGCAGTCCAA ATCAGTCTGA GTTTTTGAAT GTTACAAGCA CCATACCTTA TTATGATCCA 
GTTTCGAAAG AAAACAAAAG CAGTATTACA GAGCTGCAGT ACTTCATTGG AGAACGCTTT 
GCAAATGAAA TGTATAATGC CTGCAAAGAT GTTGAGGCTC CATCAAGTAA TGTTAAGGCA 
TTGGGGCTGC TGTGTGGGAA GGACGTCAAG GACTGCAATG CTACCAACTG GATTGAGTAC 
ATGTTTAATA AGGATAACGG ACAAACTCCT TTCAGCATTA TTCCTATCTT TTCAGATGCT 
CCTGTCCATG GAATGAATCC TATGAACAAT GCTACCAAAG GCTGTAATGA GTCTGTGGAT 
GATTCAACAG GACCATGCAG CTGTCAAGAC TGTTCAATTG TTTGTGGTCC AAAACCTCAG 
CCCCCTCCAT TACCTGCTCC TTGGCTTTTA TTTGGTTTGG ATGCTGTGTA TATCATCATG 
TGGATCTCTT ACATGGGGTT TCTACTCATA TTTTTTGCTC TAGTTTTTGG AGTCTGGTGT 
TACAGGAGGC GGCATTTTGT CTCTGAGTAT ACACCAATTG ACAGTAATGT AGCCTTTTCT 
GCGAACTCTC ATCGTGATAA TGGGAAAATT ACCTGTGGTG AGAGGCTTGG TGAAAGGTTT 
GAGAATGGCC TAAGGATGAC ATTTACATCA TGGGGTGCTT TCTGTGTCAG AAATCCGCGC 
CCGGTTATCC TCTTCTCTGT GGTCTTTATT GCTATGTGCT GTTCAGGCTT TGTATATATT 
AAAGCAACTA CAAACCCTGT AGATCTCTGG TCAGCTCCAA GCAGCCAAGC CCGCAAGGAG 
AAGGAGTACT TTGACAAACA CTTTGGGCCT TTCTTTCGTA CTGAGCAACT TATTATTCAA 
GCGCCAAACA GCCATCCAGA CATCTATTCA CCTTACCCAT CAGGAGCAGA TGTGCCTTTT 
GGACCTCCAC TCAACAAAGA TATTCTCCAT CAGGTGCTTG ATTTGCAAGA TGCTATTGTG 
AACATAGCTG CGTCTTTTGA CAATGAAACT GTTACGCTGA AAGACATTTG CTTGGCTCCT 
CTTGCTCCCT ACAACAACAA CTGCACTATA TTGAGTGTAC TCAACTACTT CCAAAACAGT 
CATTCTGTTC TAGATCACAG TGTGGGGGAT GACTTCTTTG TCTATGCTGA CTATCACACT 
CACTTCTTAT ATTGTGTTCG GGCTCCAGCA TCACTGAATG ACACAAGTTT GCTTCACGAT 
CCTTGTTTAG GAGCATTTGG TGGACCTGTC TTTCCATGGC TGGTGTTAGG GGGATACGAT 
GGTGATAACT ATAATAATGC TACTGCTCTT GTTATAACTC TTCCTGTTAA TAACTACTAC 
AATGACTCCA AGAAGATAAT GAAAGCCCTG GCGTGGGAAA AGGAGTTTAT TAACTTTTTG 
AAGAACTATG ATAATCCAAA TTTGACTATA TCATTTTCTG CGGAACGGAG CATTGAAGAC 
GAAATCAATC GTGAAAGCAG GAGTGATGTC AGCACTGTTG TAATAAGCTA TGTTGTAATG 
TTTGTGTATA TCTCCATAGC TTTGGGACAT ATCCAGAGCT GGGGAAGGCT GCTGGTGGAT 
TCAAAGATCT CCCTGGGCAT TGCAGGCATC CTGATCGTCC TGAGCTCAGT GGCATGTTCC 
ATAGGCATTT TCAGTTACTT TGGAGTTCCA GTGACACTGA TTGTGGCAGA AGTTATTCCC 
TTCTTGGTGC TGGCTATTGG GGTGGACAAC TTATTTATTA TAGTCCAGAC ACTTCAGAGA 
GATGAGCGTC TTGAGGGTGA AACTCTTGAT AAGCAGATTG GCAGAGTCCT GGGAGATGTG 
GCACCTAGCA TGTTTCTCTC ATCTTTTTCT GAGACCGTGG CATTCTTCTT AGGGACACTG 
TCTACCATGC CAGCAGTCCG CACGTTCTCC CTGTTTGCTG GAATGGCTGT GCTCATAGAT 
TTTATCCTTC AAGTCACCTG CTTTGTAAGT CTCCTGGGCT TGGATATTAG GCGCCAAGAG 
AGGAATAGAC TAGATATTCT GTGTTGCATC AAAGGCAGTG AAGAAATGAG GGGAGTCCAA 
CGCTCCGAGA GCATCTTATT TCTGTTCTTC AAAAACCTCT ATTCTCCATA TTTGCTTAAG 
GATTGGATGA GACCAATAGT GATTGCCTTG TTTGTGGGTC TTCTGTCATT CAGTACAGCA 
GTGATACACA ATGTAGAGAT TGGACTCGAT CAGTCTCTTT CAATGCCAGA TGATTCCTAT 
GTAATGAATT ACTTCAAACA GTTGGGCAAA TACATGCATG CAGGTCCTCC TGTCTACTTT 
GTTCTAGAAG AAGGGCACAA CTACACCTCT CTGGAAGGGC AAAACATGGT GTGTGGTGGA 
ACGGGATGTA ATAATGATTC ACTTGTCCAG CAAGTCTTCA ATGCTGCAGA AATTAGTAGC 
TACACAAGGA TAGGCTATGC CCCATCATCC TGGATTGATG ACTATTTTGA CTGGGTGAAA 
CCACAGTCAT CCTGTTGCAG AGTCTACAAT ACCACGGGAC AGTTCTGCAA TGCTTCAGTC 
ACAGATCCAT CCTGCACTCG TTGTCGACCA CTGAGTCAAG AAGGCAAGCA AAGACCACAG 
GGTGAAGACT TCATGACATT TTTGCCAATG TTCCTGTCTG ACAACCCTAA TCCTAAATGT 
GGCAAAGGAG GACATGCTGC ATATAGCTCA GCTGTTGACT TGATAAAGAA CAAAACTGAT 
GTTGGGGCTA CTTATTTTAT GACGTACCAT ACTGTCCTGA AAAAATCATC TGACTATATT 
GATGCTATGA AGAAGGCTCG GGACATAGCA GACAACATCA CTGAAACCAT GGGCATCAAA 
GAGAAGAACT ATCGTGTGTT TCCTTACAGT GTGTTTTATG TCTTCTATGA ACAATATTTG 
ACAATTGTGA ATGATGCCAT CTTTAACCTC TGCATCTCCT TGGGATCGAT ATTTTTGGTG 
ACAGCTGTGC TGCTTGGGTT TGAAGTATGG GCTGCTGTTG TGGTTACCGT AACGATTGCT 
ATGATAATTG TCAACATGTT TGGAGTGATG TGGCTCTGGG GCATCAGCTT GAATGCAGTT 
TCATTAGTAA ATCTCGTCAT GAGCTGTGGC ATTGCTGTGG AATTCTGCAG CCACGTGACG 
AGAGCATTCA CTGTTAGTAC TAAAGGCAGT AGAGTGGAGC GTGCAGAAGA AGCTCTGTCT 
CACATGGGCA GTTCAGTCTT CAGTGGAATC ACACTAACCA AGTTTGGAGG AATTGTAGTA 
CTGGCTTTTT CCAAGTCTCA GATCTTCAAG ATATTCTACT TCAGGATGTA TCTAGCTATG 
GTTCTGCTGG GAGCAACGCA TGGACTAATA TTTCTTCCAG TTCTGCTTAG TTACATAGGT 
CCATCAGTAA ATAAAGCTAA AACTCGTGCT GCGCAGGAGA GGTCTAGAGG CACAGAACGA 
GAGAGACTCC TCTACTTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPC1 ( XM_419162.6 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_419162.3 Gallus gallus NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X3, mRNA.

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CloneID	OGa05149
Clone ID Related Accession (Same CDS sequence)	XM_419162.5 , XM_419162.6
Accession Version	XM_419162.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	NPC intracellular cholesterol transporter 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_419162.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGGCC CGCAAGGCAG CCCCGGGCCG GGCACCCGCC TCCTGCTCCT CTTTCTCCTG 
CTGCTGCTGT CCCCGGCACA GGTACTTCCA CAACAGTGTG TCTGGTACGG AGAATGTGGA 
GTTGCATCTG GAGACAAGAG ATACAACTGT GCTTATGATG GACCACCTAT AGCTCTACCA 
AAGGGTGGCT ATGACTTGAT GCAGGAGCTC TGTCCAGGTT TATTCTTTGG CAATGTCAGT 
ACTTGCTGTG ATATCCTTCA GCTTCAGACT TTGAAAAACA ACTTGCAGCT GCCTCTGCAG 
TTTCTTTCCA GGTGTCCATC ATGTTTCTAC AACTTGATTA ACCTCTTCTG TGAACTGACT 
TGCAGTCCAA ATCAGTCTGA GTTTTTGAAT GTTACAAGCA CCATACCTTA TTATGATCCA 
GTTTCGAAAG AAAACAAAAG CAGTATTACA GAGCTGCAGT ACTTCATTGG AGAACGCTTT 
GCAAATGAAA TGTATAATGC CTGCAAAGAT GTTGAGGCTC CATCAAGTAA TGTTAAGGCA 
TTGGGGCTGC TGTGTGGGAA GGACGTCAAG GACTGCAATG CTACCAACTG GATTGAGTAC 
ATGTTTAATA AGGATAACGG ACAAACTCCT TTCAGCATTA TTCCTATCTT TTCAGATGCT 
CCTGTCCATG GAATGAATCC TATGAACAAT GCTACCAAAG GCTGTAATGA GTCTGTGGAT 
GATTCAACAG GACCATGCAG CTGTCAAGAC TGTTCAATTG TTTGTGGTCC AAAACCTCAG 
CCCCCTCCAT TACCTGCTCC TTGGCTTTTA TTTGGTTTGG ATGCTGTGTA TATCATCATG 
TGGATCTCTT ACATGGGGTT TCTACTCATA TTTTTTGCTC TAGTTTTTGG AGTCTGGTGT 
TACAGGAGGC GGCATTTTGT CTCTGAGTAT ACACCAATTG ACAGTAATGT AGCCTTTTCT 
GCGAACTCTC ATCGTGATAA TGGGAAAATT ACCTGTGGTG AGAGGCTTGG TGAAAGGTTT 
GAGAATGGCC TAAGGATGAC ATTTACATCA TGGGGTGCTT TCTGTGTCAG AAATCCGCGC 
CCGGTTATCC TCTTCTCTGT GGTCTTTATT GCTATGTGCT GTTCAGGCTT TGTATATATT 
AAAGCAACTA CAAACCCTGT AGATCTCTGG TCAGCTCCAA GCAGCCAAGC CCGCAAGGAG 
AAGGAGTACT TTGACAAACA CTTTGGGCCT TTCTTTCGTA CTGAGCAACT TATTATTCAA 
GCGCCAAACA GCCATCCAGA CATCTATTCA CCTTACCCAT CAGGAGCAGA TGTGCCTTTT 
GGACCTCCAC TCAACAAAGA TATTCTCCAT CAGGTGCTTG ATTTGCAAGA TGCTATTGTG 
AACATAGCTG CGTCTTTTGA CAATGAAACT GTTACGCTGA AAGACATTTG CTTGGCTCCT 
CTTGCTCCCT ACAACAACAA CTGCACTATA TTGAGTGTAC TCAACTACTT CCAAAACAGT 
CATTCTGTTC TAGATCACAG TGTGGGGGAT GACTTCTTTG TCTATGCTGA CTATCACACT 
CACTTCTTAT ATTGTGTTCG GGCTCCAGCA TCACTGAATG ACACAAGTTT GCTTCACGAT 
CCTTGTTTAG GAGCATTTGG TGGACCTGTC TTTCCATGGC TGGTGTTAGG GGGATACGAT 
GGTGATAACT ATAATAATGC TACTGCTCTT GTTATAACTC TTCCTGTTAA TAACTACTAC 
AATGACTCCA AGAAGATAAT GAAAGCCCTG GCGTGGGAAA AGGAGTTTAT TAACTTTTTG 
AAGAACTATG ATAATCCAAA TTTGACTATA TCATTTTCTG CGGAACGGAG CATTGAAGAC 
GAAATCAATC GTGAAAGCAG GAGTGATGTC AGCACTGTTG TAATAAGCTA TGTTGTAATG 
TTTGTGTATA TCTCCATAGC TTTGGGACAT ATCCAGAGCT GGGGAAGGCT GCTGGTGGAT 
TCAAAGATCT CCCTGGGCAT TGCAGGCATC CTGATCGTCC TGAGCTCAGT GGCATGTTCC 
ATAGGCATTT TCAGTTACTT TGGAGTTCCA GTGACACTGA TTGTGGCAGA AGTTATTCCC 
TTCTTGGTGC TGGCTATTGG GGTGGACAAC TTATTTATTA TAGTCCAGAC ACTTCAGAGA 
GATGAGCGTC TTGAGGGTGA AACTCTTGAT AAGCAGATTG GCAGAGTCCT GGGAGATGTG 
GCACCTAGCA TGTTTCTCTC ATCTTTTTCT GAGACCGTGG CATTCTTCTT AGGGACACTG 
TCTACCATGC CAGCAGTCCG CACGTTCTCC CTGTTTGCTG GAATGGCTGT GCTCATAGAT 
TTTATCCTTC AAGTCACCTG CTTTGTAAGT CTCCTGGGCT TGGATATTAG GCGCCAAGAG 
AGGAATAGAC TAGATATTCT GTGTTGCATC AAAGGCAGTG AAGAAATGAG GGGAGTCCAA 
CGCTCCGAGA GCATCTTATT TCTGTTCTTC AAAAACCTCT ATTCTCCATA TTTGCTTAAG 
GATTGGATGA GACCAATAGT GATTGCCTTG TTTGTGGGTC TTCTGTCATT CAGTACAGCA 
GTGATACACA ATGTAGAGAT TGGACTCGAT CAGTCTCTTT CAATGCCAGA TGATTCCTAT 
GTAATGAATT ACTTCAAACA GTTGGGCAAA TACATGCATG CAGGTCCTCC TGTCTACTTT 
GTTCTAGAAG AAGGGCACAA CTACACCTCT CTGGAAGGGC AAAACATGGT GTGTGGTGGA 
ACGGGATGTA ATAATGATTC ACTTGTCCAG CAAGTCTTCA ATGCTGCAGA AATTAGTAGC 
TACACAAGGA TAGGCTATGC CCCATCATCC TGGATTGATG ACTATTTTGA CTGGGTGAAA 
CCACAGTCAT CCTGTTGCAG AGTCTACAAT ACCACGGGAC AGTTCTGCAA TGCTTCAGTC 
ACAGATCCAT CCTGCACTCG TTGTCGACCA CTGAGTCAAG AAGGCAAGCA AAGACCACAG 
GGTGAAGACT TCATGACATT TTTGCCAATG TTCCTGTCTG ACAACCCTAA TCCTAAATGT 
GGCAAAGGAG GACATGCTGC ATATAGCTCA GCTGTTGACT TGATAAAGAA CAAAACTGAT 
GTTGGGGCTA CTTATTTTAT GACGTACCAT ACTGTCCTGA AAAAATCATC TGACTATATT 
GATGCTATGA AGAAGGCTCG GGACATAGCA GACAACATCA CTGAAACCAT GGGCATCAAA 
GAGAAGAACT ATCGTGTGTT TCCTTACAGT GTGTTTTATG TCTTCTATGA ACAATATTTG 
ACAATTGTGA ATGATGCCAT CTTTAACCTC TGCATCTCCT TGGGATCGAT ATTTTTGGTG 
ACAGCTGTGC TGCTTGGGTT TGAAGTATGG GCTGCTGTTG TGGTTACCGT AACGATTGCT 
ATGATAATTG TCAACATGTT TGGAGTGATG TGGCTCTGGG GCATCAGCTT GAATGCAGTT 
TCATTAGTAA ATCTCGTCAT GAGCTGTGGC ATTGCTGTGG AATTCTGCAG CCACGTGACG 
AGAGCATTCA CTGTTAGTAC TAAAGGCAGT AGAGTGGAGC GTGCAGAAGA AGCTCTGTCT 
CACATGGGCA GTTCAGTCTT CAGTGGAATC ACACTAACCA AGTTTGGAGG AATTGTAGTA 
CTGGCTTTTT CCAAGTCTCA GATCTTCAAG ATATTCTACT TCAGGATGTA TCTAGCTATG 
GTTCTGCTGG GAGCAACGCA TGGACTAATA TTTCTTCCAG TTCTGCTTAG TTACATAGGT 
CCATCAGTAA ATAAAGCTAA AACTCGTGCT GCGCAGGAGA GGTCTAGAGG CACAGAACGA 
GAGAGACTCC TCTACTTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_419162.3
CDS	399..4259
Translation	MGGPQGSPGPGTRLLLLFLLLLLSPAQVLPQQCVWYGECGVASGDKRYNCAYDGPPIALPKGGYDLMQELCPGLFFGNVSTCCDILQLQTLKNNLQLPLQFLSRCPSCFYNLINLFCELTCSPNQSEFLNVTSTIPYYDPVSKENKSSITELQYFIGERFANEMYNACKDVEAPSSNVKALGLLCGKDVKDCNATNWIEYMFNKDNGQTPFSIIPIFSDAPVHGMNPMNNATKGCNESVDDSTGPCSCQDCSIVCGPKPQPPPLPAPWLLFGLDAVYIIMWISYMGFLLIFFALVFGVWCYRRRHFVSEYTPIDSNVAFSANSHRDNGKITCGERLGERFENGLRMTFTSWGAFCVRNPRPVILFSVVFIAMCCSGFVYIKATTNPVDLWSAPSSQARKEKEYFDKHFGPFFRTEQLIIQAPNSHPDIYSPYPSGADVPFGPPLNKDILHQVLDLQDAIVNIAASFDNETVTLKDICLAPLAPYNNNCTILSVLNYFQNSHSVLDHSVGDDFFVYADYHTHFLYCVRAPASLNDTSLLHDPCLGAFGGPVFPWLVLGGYDGDNYNNATALVITLPVNNYYNDSKKIMKALAWEKEFINFLKNYDNPNLTISFSAERSIEDEINRESRSDVSTVVISYVVMFVYISIALGHIQSWGRLLVDSKISLGIAGILIVLSSVACSIGIFSYFGVPVTLIVAEVIPFLVLAIGVDNLFIIVQTLQRDERLEGETLDKQIGRVLGDVAPSMFLSSFSETVAFFLGTLSTMPAVRTFSLFAGMAVLIDFILQVTCFVSLLGLDIRRQERNRLDILCCIKGSEEMRGVQRSESILFLFFKNLYSPYLLKDWMRPIVIALFVGLLSFSTAVIHNVEIGLDQSLSMPDDSYVMNYFKQLGKYMHAGPPVYFVLEEGHNYTSLEGQNMVCGGTGCNNDSLVQQVFNAAEISSYTRIGYAPSSWIDDYFDWVKPQSSCCRVYNTTGQFCNASVTDPSCTRCRPLSQEGKQRPQGEDFMTFLPMFLSDNPNPKCGKGGHAAYSSAVDLIKNKTDVGATYFMTYHTVLKKSSDYIDAMKKARDIADNITETMGIKEKNYRVFPYSVFYVFYEQYLTIVNDAIFNLCISLGSIFLVTAVLLGFEVWAAVVVTVTIAMIIVNMFGVMWLWGISLNAVSLVNLVMSCGIAVEFCSHVTRAFTVSTKGSRVERAEEALSHMGSSVFSGITLTKFGGIVVLAFSKSQIFKIFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAKTRAAQERSRGTERERLLYF

Target ORF information:

RefSeq Version	XM_419162.6
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_419162.6

ORF Insert Sequence:

ATGGGGGGCC CGCAAGGCAG CCCCGGGCCG GGCACCCGCC TCCTGCTCCT CTTTCTCCTG 
CTGCTGCTGT CCCCGGCACA GGTACTTCCA CAACAGTGTG TCTGGTACGG AGAATGTGGA 
GTTGCATCTG GAGACAAGAG ATACAACTGT GCTTATGATG GACCACCTAT AGCTCTACCA 
AAGGGTGGCT ATGACTTGAT GCAGGAGCTC TGTCCAGGTT TATTCTTTGG CAATGTCAGT 
ACTTGCTGTG ATATCCTTCA GCTTCAGACT TTGAAAAACA ACTTGCAGCT GCCTCTGCAG 
TTTCTTTCCA GGTGTCCATC ATGTTTCTAC AACTTGATTA ACCTCTTCTG TGAACTGACT 
TGCAGTCCAA ATCAGTCTGA GTTTTTGAAT GTTACAAGCA CCATACCTTA TTATGATCCA 
GTTTCGAAAG AAAACAAAAG CAGTATTACA GAGCTGCAGT ACTTCATTGG AGAACGCTTT 
GCAAATGAAA TGTATAATGC CTGCAAAGAT GTTGAGGCTC CATCAAGTAA TGTTAAGGCA 
TTGGGGCTGC TGTGTGGGAA GGACGTCAAG GACTGCAATG CTACCAACTG GATTGAGTAC 
ATGTTTAATA AGGATAACGG ACAAACTCCT TTCAGCATTA TTCCTATCTT TTCAGATGCT 
CCTGTCCATG GAATGAATCC TATGAACAAT GCTACCAAAG GCTGTAATGA GTCTGTGGAT 
GATTCAACAG GACCATGCAG CTGTCAAGAC TGTTCAATTG TTTGTGGTCC AAAACCTCAG 
CCCCCTCCAT TACCTGCTCC TTGGCTTTTA TTTGGTTTGG ATGCTGTGTA TATCATCATG 
TGGATCTCTT ACATGGGGTT TCTACTCATA TTTTTTGCTC TAGTTTTTGG AGTCTGGTGT 
TACAGGAGGC GGCATTTTGT CTCTGAGTAT ACACCAATTG ACAGTAATGT AGCCTTTTCT 
GCGAACTCTC ATCGTGATAA TGGGAAAATT ACCTGTGGTG AGAGGCTTGG TGAAAGGTTT 
GAGAATGGCC TAAGGATGAC ATTTACATCA TGGGGTGCTT TCTGTGTCAG AAATCCGCGC 
CCGGTTATCC TCTTCTCTGT GGTCTTTATT GCTATGTGCT GTTCAGGCTT TGTATATATT 
AAAGCAACTA CAAACCCTGT AGATCTCTGG TCAGCTCCAA GCAGCCAAGC CCGCAAGGAG 
AAGGAGTACT TTGACAAACA CTTTGGGCCT TTCTTTCGTA CTGAGCAACT TATTATTCAA 
GCGCCAAACA GCCATCCAGA CATCTATTCA CCTTACCCAT CAGGAGCAGA TGTGCCTTTT 
GGACCTCCAC TCAACAAAGA TATTCTCCAT CAGGTGCTTG ATTTGCAAGA TGCTATTGTG 
AACATAGCTG CGTCTTTTGA CAATGAAACT GTTACGCTGA AAGACATTTG CTTGGCTCCT 
CTTGCTCCCT ACAACAACAA CTGCACTATA TTGAGTGTAC TCAACTACTT CCAAAACAGT 
CATTCTGTTC TAGATCACAG TGTGGGGGAT GACTTCTTTG TCTATGCTGA CTATCACACT 
CACTTCTTAT ATTGTGTTCG GGCTCCAGCA TCACTGAATG ACACAAGTTT GCTTCACGAT 
CCTTGTTTAG GAGCATTTGG TGGACCTGTC TTTCCATGGC TGGTGTTAGG GGGATACGAT 
GGTGATAACT ATAATAATGC TACTGCTCTT GTTATAACTC TTCCTGTTAA TAACTACTAC 
AATGACTCCA AGAAGATAAT GAAAGCCCTG GCGTGGGAAA AGGAGTTTAT TAACTTTTTG 
AAGAACTATG ATAATCCAAA TTTGACTATA TCATTTTCTG CGGAACGGAG CATTGAAGAC 
GAAATCAATC GTGAAAGCAG GAGTGATGTC AGCACTGTTG TAATAAGCTA TGTTGTAATG 
TTTGTGTATA TCTCCATAGC TTTGGGACAT ATCCAGAGCT GGGGAAGGCT GCTGGTGGAT 
TCAAAGATCT CCCTGGGCAT TGCAGGCATC CTGATCGTCC TGAGCTCAGT GGCATGTTCC 
ATAGGCATTT TCAGTTACTT TGGAGTTCCA GTGACACTGA TTGTGGCAGA AGTTATTCCC 
TTCTTGGTGC TGGCTATTGG GGTGGACAAC TTATTTATTA TAGTCCAGAC ACTTCAGAGA 
GATGAGCGTC TTGAGGGTGA AACTCTTGAT AAGCAGATTG GCAGAGTCCT GGGAGATGTG 
GCACCTAGCA TGTTTCTCTC ATCTTTTTCT GAGACCGTGG CATTCTTCTT AGGGACACTG 
TCTACCATGC CAGCAGTCCG CACGTTCTCC CTGTTTGCTG GAATGGCTGT GCTCATAGAT 
TTTATCCTTC AAGTCACCTG CTTTGTAAGT CTCCTGGGCT TGGATATTAG GCGCCAAGAG 
AGGAATAGAC TAGATATTCT GTGTTGCATC AAAGGCAGTG AAGAAATGAG GGGAGTCCAA 
CGCTCCGAGA GCATCTTATT TCTGTTCTTC AAAAACCTCT ATTCTCCATA TTTGCTTAAG 
GATTGGATGA GACCAATAGT GATTGCCTTG TTTGTGGGTC TTCTGTCATT CAGTACAGCA 
GTGATACACA ATGTAGAGAT TGGACTCGAT CAGTCTCTTT CAATGCCAGA TGATTCCTAT 
GTAATGAATT ACTTCAAACA GTTGGGCAAA TACATGCATG CAGGTCCTCC TGTCTACTTT 
GTTCTAGAAG AAGGGCACAA CTACACCTCT CTGGAAGGGC AAAACATGGT GTGTGGTGGA 
ACGGGATGTA ATAATGATTC ACTTGTCCAG CAAGTCTTCA ATGCTGCAGA AATTAGTAGC 
TACACAAGGA TAGGCTATGC CCCATCATCC TGGATTGATG ACTATTTTGA CTGGGTGAAA 
CCACAGTCAT CCTGTTGCAG AGTCTACAAT ACCACGGGAC AGTTCTGCAA TGCTTCAGTC 
ACAGATCCAT CCTGCACTCG TTGTCGACCA CTGAGTCAAG AAGGCAAGCA AAGACCACAG 
GGTGAAGACT TCATGACATT TTTGCCAATG TTCCTGTCTG ACAACCCTAA TCCTAAATGT 
GGCAAAGGAG GACATGCTGC ATATAGCTCA GCTGTTGACT TGATAAAGAA CAAAACTGAT 
GTTGGGGCTA CTTATTTTAT GACGTACCAT ACTGTCCTGA AAAAATCATC TGACTATATT 
GATGCTATGA AGAAGGCTCG GGACATAGCA GACAACATCA CTGAAACCAT GGGCATCAAA 
GAGAAGAACT ATCGTGTGTT TCCTTACAGT GTGTTTTATG TCTTCTATGA ACAATATTTG 
ACAATTGTGA ATGATGCCAT CTTTAACCTC TGCATCTCCT TGGGATCGAT ATTTTTGGTG 
ACAGCTGTGC TGCTTGGGTT TGAAGTATGG GCTGCTGTTG TGGTTACCGT AACGATTGCT 
ATGATAATTG TCAACATGTT TGGAGTGATG TGGCTCTGGG GCATCAGCTT GAATGCAGTT 
TCATTAGTAA ATCTCGTCAT GAGCTGTGGC ATTGCTGTGG AATTCTGCAG CCACGTGACG 
AGAGCATTCA CTGTTAGTAC TAAAGGCAGT AGAGTGGAGC GTGCAGAAGA AGCTCTGTCT 
CACATGGGCA GTTCAGTCTT CAGTGGAATC ACACTAACCA AGTTTGGAGG AATTGTAGTA 
CTGGCTTTTT CCAAGTCTCA GATCTTCAAG ATATTCTACT TCAGGATGTA TCTAGCTATG 
GTTCTGCTGG GAGCAACGCA TGGACTAATA TTTCTTCCAG TTCTGCTTAG TTACATAGGT 
CCATCAGTAA ATAAAGCTAA AACTCGTGCT GCGCAGGAGA GGTCTAGAGG CACAGAACGA 
GAGAGACTCC TCTACTTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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NPC1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

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