COBL cDNA ORF clone, Gallus gallus(chicken)

The following COBL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COBL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa32267 XM_015282312.2
Latest version!
Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa32268 XM_025147765.1
Latest version!
Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa32268 XM_015282313.2
Latest version!
Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa32268 XM_015282314.2
Latest version!
Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa32269 XM_015282315.2
Latest version!
Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa32270 XM_015282316.2
Latest version!
Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa32271 XM_015282317.2
Latest version!
Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa32272 XM_015282318.2
Latest version!
Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa32272 XM_015282319.2
Latest version!
Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa04923 XM_004935146.3
Latest version!
Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OGa32272 XM_015282318.1 Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa32271 XM_015282317.1 Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa32267 XM_015282312.1 Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa32270 XM_015282316.1 Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa32268 XM_015282314.1 Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa32272 XM_015282319.1 Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa32268 XM_015282313.1 Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa04923 XM_004935146.2 Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OGa32269 XM_015282315.1 Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa32267
    Clone ID Related Accession (Same CDS sequence) XM_015282312.2 , XM_015282312.1
    Accession Version XM_015282312.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4194bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein cordon-bleu isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015282312.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGACTC TCGTCCGCCC CACGGCCGGC AAGCCGCCCA GCGGGAGAAG AATGAAAGCT 
    CGTGCTCCTC CTCCACCAAA CCAACCTTCT GCAACCAGTA GAATTCACAG TGAGCACAAG
    TCACCTGCAG AGACAGCCGT AATTTCTGAT CAGAGCCTTG TGAGCATGAA GGAGAATATG
    ATAAATAGAA TGGTGGACTT CACAGTCATT TTACCCAGCG GGGTGGAACA GAAGAGCACA
    GTACAAGGAA GTAAAGCTGT GATGGATCTT TTGGTTGATC TATGCAGCCG GTATCACTTG
    AATCCATCCC AGTACAGTCT TGAATTGAAG TCTTTGGAAA CTCAACAACC TTTGAGTTAC
    AAGCCAAACA CTTTGATAGG AGCACTGGAC GTACAGACAG TACTCCTGAA AGAGAAGGTT
    CCTGAAGAGA AGATCAAGAG ACCTCTGCCG AGGGCTCCTG AGAAGTCTGT GAGGCTGGTG
    GTAAATTACC TGAAGACACA GAAGGCAGTG GTTCGAGTCA GCCCGGACGT GCCCCTTCAC
    AACATCATCC CAGCTATTTG TGAAAAGTGT GAGGTCAGTC AGGAGCACAT TGTTCTTCTG
    CGAGACAGCA TCACTGGCGA GGAGCTGGAG CTTACCAAAT CCTTGGAGGA GCTGGGAATA
    AGCGAGCTGT ACGCCTGGGA CCGAAAAAGA GAACCAAGTC GAAGTGTTTC ACTAAGCAAC
    GATGCAATAG AAAAAGAAAA GAAAGGATTT CTGGGGTTTT TCAAAGCTAA TAGAAGCAAC
    AGCAAGACAG AAGAACACTT GAGGATGAAC AGAGATTATG GTGAGGAGGA TGTTTTTAGT
    TCTACATCTA CAAGTGAAGG AAGCTTGGAT GGTTTTTCCA CAGCACCAAA CTCCCCTTCG
    GTGAATTCCC GTTCCAGTAC CTTGGGTCCA TCGCTGTCCC TTGGTAATAT CTCAGGAATG
    ACAGCAAACC CAGAAGTGAA AAAGCGCAGA GCACCTCCTC CACCAGTTTT GACACCTCCG
    TTGCAGAATA TGGAGATGAG CTCCGCTCAG GAGAAGACAG CAGTACAGAT TTCACAAGGA
    GCATCTCTTA ACGATCTACA GAAAAAGAAG AGGCGTGCAC CTCTTCCACC AACTCAGTCT
    GCTCCACCTA CTCCAGCCAT GCCAAATAGG AGGGAAGAGA TGGAAGACAA GAGGAAGAGC
    ACAATGGGTG ATGGACGGCA GGTGCCACAA AAGCCTCCTA GGGGCCCCAC TCGTGACCCA
    CCCCAGTTAG TGATCCCCCC ACCCCCACCT TACCCTCCTC CTGAAAGAGA CAATATGGAT
    CCAGCAGTCT CTTACAGAGA AGCAGATGTT ACAGCACCAA CAAAACTGGT ACCTAAACAG
    AGCCTGCTGC CTGCACATGA TAATTTTTAT GTAGTGGATG ATACAGTTAT GGAGCTATCA
    GAGGTAGAAG AAACAGCGTC AGAAAGCAGC TGCTTTGCAT CTGAGGACAC TACCGAAGAC
    TCAGGTGTAG TGAGCTCCCC ATCTGACATT GTGTCACTGG ATTCACAAAA TGACAGTATG
    AAGTTGAGAG ACATCAAGCT GGTGAATGGC TATGTGGACT CAGACACGAT CTATGGTGCA
    GAGACTTGCT CTGTGAAGAA CGCGTACTGC GATGATATGG GATATGCCGG TACTCCACAG
    AGCACTAAAA ATGAAGAAAT GGCTGCGGCT AAGCATGAGA ATGAGGACAT ATTCATTGAT
    GCACAACTCC AGCAGACTTT GGCTGACCTG GATGAAGATT TAGAAGCTAT GGATGTTTTA
    AGTAACTCTG ACTCTGAGCA CATGAGTGAA GTGTTGAGCC CACATTCAGG AAAAGTTGAG
    GTCCCCCAAG ATGTTGCTAG TTCTGTTCCA GTAACAGTCA TAGATGATGC TCCAGAAGTT
    ACCATCTATA ACTCAGCTGG AAACCAAGAG ACAAGGGGTT CACAGAATCT ATCAGCTGAC
    TCTGTTAATA TGGGAAACTT CACAAATAGA AACAACAATG CAGGTTCCTT TGATAAGGGA
    CACACTGATG GTGGAGCTGT ATGTATACCC AAGGACCTAA TACATCAGCA GCCATCTGAT
    AATGAGTTCA GTCACATACC CGTGGAACAG AGGAGAGATA TGTTTGAATC TCTCACGTCC
    TCCTTTGAAA AAAGAAGTGA AAAGAATGTA AGAGTAGAAG CTCCGCCCAG ACACGTTATG
    AAGTCTGAGG AGTGTAAATC GAAGCTGACG TCATCTCACT CCAAGACTGC GACTGAAATC
    AATACAGCAA AAGAGAAAGT AAATCCATTT AATGAATGTA GTAAGAGGGA GAGACCTCTG
    AAAAAAAGTA AGTCAGAACT AGAAATCAAA AGGCCACCAG CAAAAAAGAT TGAAGTAGAA
    GAAGTTCCAT CAACACCTCC GTGGTGTCAT CGTGCCCAGA AATTGGAAAC AAGTTATGAA
    CCCAAAATGG GTTTGACGAC CTTCAAAGTT GTCCCTCCCA AACCTGAAGT AAAAAATTTT
    GATAGAGGGC TTTCAGTCTC CACTGGTGCT ATTAAGATTG ATGAACTAGG CAACCTTATC
    AACCCAAAGC CTGTTGTCAG TAAGAACATA CCAGGTACCT CTGCTGGTGA AAGTGAGGAA
    ATTCCTCTTG GGAAAGTGAA GGCATTCTGG AGGTCAAGTT CTATGGAAAA ACAAAGTGAT
    GACTCTGCTG AGCATCTTGC TAAAAAGCCA GCAGTTACCA CAAACTCTAA GTCCTTTGTT
    ACAAAGCAAG AATGCAAACC TGTCTGTCTT GCAGCTCCAA AACCTGTACC ACCACAAACT
    GTTATTTCCC AGGTAGGTGA AAAGCAGTCT GAGAATGAGA AGACCAAACC ACCTCTTCCA
    GTTACACAGT CTCAGGTAAA ACCATTAGTG GTCCCAGCCA TTAATAAGGA CAAGCCGGAG
    CTTCCATTTC TAAAGCCTCA CAGAAGAACT TCCAGTCAGT ATGTGGCCTC TGCCATTGCA
    AAACACATCA ATCTGCCTAC CTTTAAGACT GATTCTACAG ATTTGCATGA GAAAGATGAA
    AATAAGCACG GGGCAGGAGA GGTTAAAACT GAAGCTGAAG TTTCCCCCAA AAGGTGTATT
    ATTGTGGCCA AGTACAGCCC TGTGGAAACA GAATCAGCAG AAACAAGAGA AAAAATTTCA
    AGTATTTTTA CTTGTAGTCA AAAAGCATCC CACAGATTCA CACCTGGTGA TAATTCTGGC
    ATGGAAAACA AAGTAGTGAA CATCAGGGCA CCGAGTCAAG CAACTCCTGC TAGTTTCTAT
    AAAAAGAATG CCAGTGTCCC ACTTACTAAA TCCTCTTCAA AGGATAACAA CAAAGCAGAA
    GATGATCACA GTTTAAACAG CAAACAAGGT ACAGCAGAGA AAAAGATGCA TCTTTTGTTT
    GACCAGAAAT ACGAGACTTT GCCCCGTAGT AATTCAGCCT CCTCTCTCAA GTCACCTGAT
    CTCCAAGGAG CCTCATCTTC TTTCAGTTCA TCTTTGCGCA TCACTAAATG GCCTCCTGCT
    GACAGCGTGC AAGTTAGTTC CCTGAAAGCA TCGCCCGAGT TCAGTGGTGT GACAGCAAAG
    GCAGAATCAG AACAGAATGA CAAATCTGAT GATTCGGCTG CTAATGCTAT TGGCGAATTG
    GATGTTAATG TGCAGACTAA TATCTTCGGA CCAAAGAAGA AGTTCAAACC TGTTGTCCAA
    AAACCAATTC CGAAAGATAC ATCACTGCAC AGTGCCCTCA TGGAAGCTAT TCAAACAGGA
    GGGGGAAAAG AGAAACTCAG AAAGGTTTCA GACAGCGCGC TAAATGGCAA TCACAAGAAA
    CCATCATACA GTGAGCCAGA GAATGAGCGG TCGGCCCTAC TAGCGGCAAT TAGAGGCCAC
    AGTGGCACTT GCAAGCTGAA AAAAATCTCC TCATTGGCCT CCGAAGAGCT CCAGAGTTTT
    AGGAATGCAG AACTGTCTTC ACAGAAAGCA GAAGACCCTC AGGAAGAGCA GCTCTGCATT
    CCACCTGCCC CTGCCCAGCC ACCACCACCG CCTCCCCCTC AGCTGTCAAC AGCAACTTTC
    AAATTCTCTG CCACAACTAC GGGTGACCCT GGAGAGGCCA GGCGAGCCCT GATGGAGGCC
    ATTCGCTCTG GTGCTGGAGC AGCAAAGTTA AAAAAGGTCC CTCTGCTCGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137798.1
    CDS368..4561
    Translation

    Target ORF information:

    RefSeq Version XM_015282312.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282312.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGACTC TCGTCCGCCC CACGGCCGGC AAGCCGCCCA GCGGGAGAAG AATGAAAGCT 
    CGTGCTCCTC CTCCACCAAA CCAACCTTCT GCAACCAGTA GAATTCACAG TGAGCACAAG
    TCACCTGCAG AGACAGCCGT AATTTCTGAT CAGAGCCTTG TGAGCATGAA GGAGAATATG
    ATAAATAGAA TGGTGGACTT CACAGTCATT TTACCCAGCG GGGTGGAACA GAAGAGCACA
    GTACAAGGAA GTAAAGCTGT GATGGATCTT TTGGTTGATC TATGCAGCCG GTATCACTTG
    AATCCATCCC AGTACAGTCT TGAATTGAAG TCTTTGGAAA CTCAACAACC TTTGAGTTAC
    AAGCCAAACA CTTTGATAGG AGCACTGGAC GTACAGACAG TACTCCTGAA AGAGAAGGTT
    CCTGAAGAGA AGATCAAGAG ACCTCTGCCG AGGGCTCCTG AGAAGTCTGT GAGGCTGGTG
    GTAAATTACC TGAAGACACA GAAGGCAGTG GTTCGAGTCA GCCCGGACGT GCCCCTTCAC
    AACATCATCC CAGCTATTTG TGAAAAGTGT GAGGTCAGTC AGGAGCACAT TGTTCTTCTG
    CGAGACAGCA TCACTGGCGA GGAGCTGGAG CTTACCAAAT CCTTGGAGGA GCTGGGAATA
    AGCGAGCTGT ACGCCTGGGA CCGAAAAAGA GAACCAAGTC GAAGTGTTTC ACTAAGCAAC
    GATGCAATAG AAAAAGAAAA GAAAGGATTT CTGGGGTTTT TCAAAGCTAA TAGAAGCAAC
    AGCAAGACAG AAGAACACTT GAGGATGAAC AGAGATTATG GTGAGGAGGA TGTTTTTAGT
    TCTACATCTA CAAGTGAAGG AAGCTTGGAT GGTTTTTCCA CAGCACCAAA CTCCCCTTCG
    GTGAATTCCC GTTCCAGTAC CTTGGGTCCA TCGCTGTCCC TTGGTAATAT CTCAGGAATG
    ACAGCAAACC CAGAAGTGAA AAAGCGCAGA GCACCTCCTC CACCAGTTTT GACACCTCCG
    TTGCAGAATA TGGAGATGAG CTCCGCTCAG GAGAAGACAG CAGTACAGAT TTCACAAGGA
    GCATCTCTTA ACGATCTACA GAAAAAGAAG AGGCGTGCAC CTCTTCCACC AACTCAGTCT
    GCTCCACCTA CTCCAGCCAT GCCAAATAGG AGGGAAGAGA TGGAAGACAA GAGGAAGAGC
    ACAATGGGTG ATGGACGGCA GGTGCCACAA AAGCCTCCTA GGGGCCCCAC TCGTGACCCA
    CCCCAGTTAG TGATCCCCCC ACCCCCACCT TACCCTCCTC CTGAAAGAGA CAATATGGAT
    CCAGCAGTCT CTTACAGAGA AGCAGATGTT ACAGCACCAA CAAAACTGGT ACCTAAACAG
    AGCCTGCTGC CTGCACATGA TAATTTTTAT GTAGTGGATG ATACAGTTAT GGAGCTATCA
    GAGGTAGAAG AAACAGCGTC AGAAAGCAGC TGCTTTGCAT CTGAGGACAC TACCGAAGAC
    TCAGGTGTAG TGAGCTCCCC ATCTGACATT GTGTCACTGG ATTCACAAAA TGACAGTATG
    AAGTTGAGAG ACATCAAGCT GGTGAATGGC TATGTGGACT CAGACACGAT CTATGGTGCA
    GAGACTTGCT CTGTGAAGAA CGCGTACTGC GATGATATGG GATATGCCGG TACTCCACAG
    AGCACTAAAA ATGAAGAAAT GGCTGCGGCT AAGCATGAGA ATGAGGACAT ATTCATTGAT
    GCACAACTCC AGCAGACTTT GGCTGACCTG GATGAAGATT TAGAAGCTAT GGATGTTTTA
    AGTAACTCTG ACTCTGAGCA CATGAGTGAA GTGTTGAGCC CACATTCAGG AAAAGTTGAG
    GTCCCCCAAG ATGTTGCTAG TTCTGTTCCA GTAACAGTCA TAGATGATGC TCCAGAAGTT
    ACCATCTATA ACTCAGCTGG AAACCAAGAG ACAAGGGGTT CACAGAATCT ATCAGCTGAC
    TCTGTTAATA TGGGAAACTT CACAAATAGA AACAACAATG CAGGTTCCTT TGATAAGGGA
    CACACTGATG GTGGAGCTGT ATGTATACCC AAGGACCTAA TACATCAGCA GCCATCTGAT
    AATGAGTTCA GTCACATACC CGTGGAACAG AGGAGAGATA TGTTTGAATC TCTCACGTCC
    TCCTTTGAAA AAAGAAGTGA AAAGAATGTA AGAGTAGAAG CTCCGCCCAG ACACGTTATG
    AAGTCTGAGG AGTGTAAATC GAAGCTGACG TCATCTCACT CCAAGACTGC GACTGAAATC
    AATACAGCAA AAGAGAAAGT AAATCCATTT AATGAATGTA GTAAGAGGGA GAGACCTCTG
    AAAAAAAGTA AGTCAGAACT AGAAATCAAA AGGCCACCAG CAAAAAAGAT TGAAGTAGAA
    GAAGTTCCAT CAACACCTCC GTGGTGTCAT CGTGCCCAGA AATTGGAAAC AAGTTATGAA
    CCCAAAATGG GTTTGACGAC CTTCAAAGTT GTCCCTCCCA AACCTGAAGT AAAAAATTTT
    GATAGAGGGC TTTCAGTCTC CACTGGTGCT ATTAAGATTG ATGAACTAGG CAACCTTATC
    AACCCAAAGC CTGTTGTCAG TAAGAACATA CCAGGTACCT CTGCTGGTGA AAGTGAGGAA
    ATTCCTCTTG GGAAAGTGAA GGCATTCTGG AGGTCAAGTT CTATGGAAAA ACAAAGTGAT
    GACTCTGCTG AGCATCTTGC TAAAAAGCCA GCAGTTACCA CAAACTCTAA GTCCTTTGTT
    ACAAAGCAAG AATGCAAACC TGTCTGTCTT GCAGCTCCAA AACCTGTACC ACCACAAACT
    GTTATTTCCC AGGTAGGTGA AAAGCAGTCT GAGAATGAGA AGACCAAACC ACCTCTTCCA
    GTTACACAGT CTCAGGTAAA ACCATTAGTG GTCCCAGCCA TTAATAAGGA CAAGCCGGAG
    CTTCCATTTC TAAAGCCTCA CAGAAGAACT TCCAGTCAGT ATGTGGCCTC TGCCATTGCA
    AAACACATCA ATCTGCCTAC CTTTAAGACT GATTCTACAG ATTTGCATGA GAAAGATGAA
    AATAAGCACG GGGCAGGAGA GGTTAAAACT GAAGCTGAAG TTTCCCCCAA AAGGTGTATT
    ATTGTGGCCA AGTACAGCCC TGTGGAAACA GAATCAGCAG AAACAAGAGA AAAAATTTCA
    AGTATTTTTA CTTGTAGTCA AAAAGCATCC CACAGATTCA CACCTGGTGA TAATTCTGGC
    ATGGAAAACA AAGTAGTGAA CATCAGGGCA CCGAGTCAAG CAACTCCTGC TAGTTTCTAT
    AAAAAGAATG CCAGTGTCCC ACTTACTAAA TCCTCTTCAA AGGATAACAA CAAAGCAGAA
    GATGATCACA GTTTAAACAG CAAACAAGGT ACAGCAGAGA AAAAGATGCA TCTTTTGTTT
    GACCAGAAAT ACGAGACTTT GCCCCGTAGT AATTCAGCCT CCTCTCTCAA GTCACCTGAT
    CTCCAAGGAG CCTCATCTTC TTTCAGTTCA TCTTTGCGCA TCACTAAATG GCCTCCTGCT
    GACAGCGTGC AAGTTAGTTC CCTGAAAGCA TCGCCCGAGT TCAGTGGTGT GACAGCAAAG
    GCAGAATCAG AACAGAATGA CAAATCTGAT GATTCGGCTG CTAATGCTAT TGGCGAATTG
    GATGTTAATG TGCAGACTAA TATCTTCGGA CCAAAGAAGA AGTTCAAACC TGTTGTCCAA
    AAACCAATTC CGAAAGATAC ATCACTGCAC AGTGCCCTCA TGGAAGCTAT TCAAACAGGA
    GGGGGAAAAG AGAAACTCAG AAAGGTTTCA GACAGCGCGC TAAATGGCAA TCACAAGAAA
    CCATCATACA GTGAGCCAGA GAATGAGCGG TCGGCCCTAC TAGCGGCAAT TAGAGGCCAC
    AGTGGCACTT GCAAGCTGAA AAAAATCTCC TCATTGGCCT CCGAAGAGCT CCAGAGTTTT
    AGGAATGCAG AACTGTCTTC ACAGAAAGCA GAAGACCCTC AGGAAGAGCA GCTCTGCATT
    CCACCTGCCC CTGCCCAGCC ACCACCACCG CCTCCCCCTC AGCTGTCAAC AGCAACTTTC
    AAATTCTCTG CCACAACTAC GGGTGACCCT GGAGAGGCCA GGCGAGCCCT GATGGAGGCC
    ATTCGCTCTG GTGCTGGAGC AGCAAAGTTA AAAAAGGTCC CTCTGCTCGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32267
    Clone ID Related Accession (Same CDS sequence) XM_015282312.2 , XM_015282312.1
    Accession Version XM_015282312.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4194bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product protein cordon-bleu isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455789.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGACTC TCGTCCGCCC CACGGCCGGC AAGCCGCCCA GCGGGAGAAG AATGAAAGCT 
    CGTGCTCCTC CTCCACCAAA CCAACCTTCT GCAACCAGTA GAATTCACAG TGAGCACAAG
    TCACCTGCAG AGACAGCCGT AATTTCTGAT CAGAGCCTTG TGAGCATGAA GGAGAATATG
    ATAAATAGAA TGGTGGACTT CACAGTCATT TTACCCAGCG GGGTGGAACA GAAGAGCACA
    GTACAAGGAA GTAAAGCTGT GATGGATCTT TTGGTTGATC TATGCAGCCG GTATCACTTG
    AATCCATCCC AGTACAGTCT TGAATTGAAG TCTTTGGAAA CTCAACAACC TTTGAGTTAC
    AAGCCAAACA CTTTGATAGG AGCACTGGAC GTACAGACAG TACTCCTGAA AGAGAAGGTT
    CCTGAAGAGA AGATCAAGAG ACCTCTGCCG AGGGCTCCTG AGAAGTCTGT GAGGCTGGTG
    GTAAATTACC TGAAGACACA GAAGGCAGTG GTTCGAGTCA GCCCGGACGT GCCCCTTCAC
    AACATCATCC CAGCTATTTG TGAAAAGTGT GAGGTCAGTC AGGAGCACAT TGTTCTTCTG
    CGAGACAGCA TCACTGGCGA GGAGCTGGAG CTTACCAAAT CCTTGGAGGA GCTGGGAATA
    AGCGAGCTGT ACGCCTGGGA CCGAAAAAGA GAACCAAGTC GAAGTGTTTC ACTAAGCAAC
    GATGCAATAG AAAAAGAAAA GAAAGGATTT CTGGGGTTTT TCAAAGCTAA TAGAAGCAAC
    AGCAAGACAG AAGAACACTT GAGGATGAAC AGAGATTATG GTGAGGAGGA TGTTTTTAGT
    TCTACATCTA CAAGTGAAGG AAGCTTGGAT GGTTTTTCCA CAGCACCAAA CTCCCCTTCG
    GTGAATTCCC GTTCCAGTAC CTTGGGTCCA TCGCTGTCCC TTGGTAATAT CTCAGGAATG
    ACAGCAAACC CAGAAGTGAA AAAGCGCAGA GCACCTCCTC CACCAGTTTT GACACCTCCG
    TTGCAGAATA TGGAGATGAG CTCCGCTCAG GAGAAGACAG CAGTACAGAT TTCACAAGGA
    GCATCTCTTA ACGATCTACA GAAAAAGAAG AGGCGTGCAC CTCTTCCACC AACTCAGTCT
    GCTCCACCTA CTCCAGCCAT GCCAAATAGG AGGGAAGAGA TGGAAGACAA GAGGAAGAGC
    ACAATGGGTG ATGGACGGCA GGTGCCACAA AAGCCTCCTA GGGGCCCCAC TCGTGACCCA
    CCCCAGTTAG TGATCCCCCC ACCCCCACCT TACCCTCCTC CTGAAAGAGA CAATATGGAT
    CCAGCAGTCT CTTACAGAGA AGCAGATGTT ACAGCACCAA CAAAACTGGT ACCTAAACAG
    AGCCTGCTGC CTGCACATGA TAATTTTTAT GTAGTGGATG ATACAGTTAT GGAGCTATCA
    GAGGTAGAAG AAACAGCGTC AGAAAGCAGC TGCTTTGCAT CTGAGGACAC TACCGAAGAC
    TCAGGTGTAG TGAGCTCCCC ATCTGACATT GTGTCACTGG ATTCACAAAA TGACAGTATG
    AAGTTGAGAG ACATCAAGCT GGTGAATGGC TATGTGGACT CAGACACGAT CTATGGTGCA
    GAGACTTGCT CTGTGAAGAA CGCGTACTGC GATGATATGG GATATGCCGG TACTCCACAG
    AGCACTAAAA ATGAAGAAAT GGCTGCGGCT AAGCATGAGA ATGAGGACAT ATTCATTGAT
    GCACAACTCC AGCAGACTTT GGCTGACCTG GATGAAGATT TAGAAGCTAT GGATGTTTTA
    AGTAACTCTG ACTCTGAGCA CATGAGTGAA GTGTTGAGCC CACATTCAGG AAAAGTTGAG
    GTCCCCCAAG ATGTTGCTAG TTCTGTTCCA GTAACAGTCA TAGATGATGC TCCAGAAGTT
    ACCATCTATA ACTCAGCTGG AAACCAAGAG ACAAGGGGTT CACAGAATCT ATCAGCTGAC
    TCTGTTAATA TGGGAAACTT CACAAATAGA AACAACAATG CAGGTTCCTT TGATAAGGGA
    CACACTGATG GTGGAGCTGT ATGTATACCC AAGGACCTAA TACATCAGCA GCCATCTGAT
    AATGAGTTCA GTCACATACC CGTGGAACAG AGGAGAGATA TGTTTGAATC TCTCACGTCC
    TCCTTTGAAA AAAGAAGTGA AAAGAATGTA AGAGTAGAAG CTCCGCCCAG ACACGTTATG
    AAGTCTGAGG AGTGTAAATC GAAGCTGACG TCATCTCACT CCAAGACTGC GACTGAAATC
    AATACAGCAA AAGAGAAAGT AAATCCATTT AATGAATGTA GTAAGAGGGA GAGACCTCTG
    AAAAAAAGTA AGTCAGAACT AGAAATCAAA AGGCCACCAG CAAAAAAGAT TGAAGTAGAA
    GAAGTTCCAT CAACACCTCC GTGGTGTCAT CGTGCCCAGA AATTGGAAAC AAGTTATGAA
    CCCAAAATGG GTTTGACGAC CTTCAAAGTT GTCCCTCCCA AACCTGAAGT AAAAAATTTT
    GATAGAGGGC TTTCAGTCTC CACTGGTGCT ATTAAGATTG ATGAACTAGG CAACCTTATC
    AACCCAAAGC CTGTTGTCAG TAAGAACATA CCAGGTACCT CTGCTGGTGA AAGTGAGGAA
    ATTCCTCTTG GGAAAGTGAA GGCATTCTGG AGGTCAAGTT CTATGGAAAA ACAAAGTGAT
    GACTCTGCTG AGCATCTTGC TAAAAAGCCA GCAGTTACCA CAAACTCTAA GTCCTTTGTT
    ACAAAGCAAG AATGCAAACC TGTCTGTCTT GCAGCTCCAA AACCTGTACC ACCACAAACT
    GTTATTTCCC AGGTAGGTGA AAAGCAGTCT GAGAATGAGA AGACCAAACC ACCTCTTCCA
    GTTACACAGT CTCAGGTAAA ACCATTAGTG GTCCCAGCCA TTAATAAGGA CAAGCCGGAG
    CTTCCATTTC TAAAGCCTCA CAGAAGAACT TCCAGTCAGT ATGTGGCCTC TGCCATTGCA
    AAACACATCA ATCTGCCTAC CTTTAAGACT GATTCTACAG ATTTGCATGA GAAAGATGAA
    AATAAGCACG GGGCAGGAGA GGTTAAAACT GAAGCTGAAG TTTCCCCCAA AAGGTGTATT
    ATTGTGGCCA AGTACAGCCC TGTGGAAACA GAATCAGCAG AAACAAGAGA AAAAATTTCA
    AGTATTTTTA CTTGTAGTCA AAAAGCATCC CACAGATTCA CACCTGGTGA TAATTCTGGC
    ATGGAAAACA AAGTAGTGAA CATCAGGGCA CCGAGTCAAG CAACTCCTGC TAGTTTCTAT
    AAAAAGAATG CCAGTGTCCC ACTTACTAAA TCCTCTTCAA AGGATAACAA CAAAGCAGAA
    GATGATCACA GTTTAAACAG CAAACAAGGT ACAGCAGAGA AAAAGATGCA TCTTTTGTTT
    GACCAGAAAT ACGAGACTTT GCCCCGTAGT AATTCAGCCT CCTCTCTCAA GTCACCTGAT
    CTCCAAGGAG CCTCATCTTC TTTCAGTTCA TCTTTGCGCA TCACTAAATG GCCTCCTGCT
    GACAGCGTGC AAGTTAGTTC CCTGAAAGCA TCGCCCGAGT TCAGTGGTGT GACAGCAAAG
    GCAGAATCAG AACAGAATGA CAAATCTGAT GATTCGGCTG CTAATGCTAT TGGCGAATTG
    GATGTTAATG TGCAGACTAA TATCTTCGGA CCAAAGAAGA AGTTCAAACC TGTTGTCCAA
    AAACCAATTC CGAAAGATAC ATCACTGCAC AGTGCCCTCA TGGAAGCTAT TCAAACAGGA
    GGGGGAAAAG AGAAACTCAG AAAGGTTTCA GACAGCGCGC TAAATGGCAA TCACAAGAAA
    CCATCATACA GTGAGCCAGA GAATGAGCGG TCGGCCCTAC TAGCGGCAAT TAGAGGCCAC
    AGTGGCACTT GCAAGCTGAA AAAAATCTCC TCATTGGCCT CCGAAGAGCT CCAGAGTTTT
    AGGAATGCAG AACTGTCTTC ACAGAAAGCA GAAGACCCTC AGGAAGAGCA GCTCTGCATT
    CCACCTGCCC CTGCCCAGCC ACCACCACCG CCTCCCCCTC AGCTGTCAAC AGCAACTTTC
    AAATTCTCTG CCACAACTAC GGGTGACCCT GGAGAGGCCA GGCGAGCCCT GATGGAGGCC
    ATTCGCTCTG GTGCTGGAGC AGCAAAGTTA AAAAAGGTCC CTCTGCTCGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137798.1
    CDS194..4387
    Translation

    Target ORF information:

    RefSeq Version XM_015282312.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282312.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGACTC TCGTCCGCCC CACGGCCGGC AAGCCGCCCA GCGGGAGAAG AATGAAAGCT 
    CGTGCTCCTC CTCCACCAAA CCAACCTTCT GCAACCAGTA GAATTCACAG TGAGCACAAG
    TCACCTGCAG AGACAGCCGT AATTTCTGAT CAGAGCCTTG TGAGCATGAA GGAGAATATG
    ATAAATAGAA TGGTGGACTT CACAGTCATT TTACCCAGCG GGGTGGAACA GAAGAGCACA
    GTACAAGGAA GTAAAGCTGT GATGGATCTT TTGGTTGATC TATGCAGCCG GTATCACTTG
    AATCCATCCC AGTACAGTCT TGAATTGAAG TCTTTGGAAA CTCAACAACC TTTGAGTTAC
    AAGCCAAACA CTTTGATAGG AGCACTGGAC GTACAGACAG TACTCCTGAA AGAGAAGGTT
    CCTGAAGAGA AGATCAAGAG ACCTCTGCCG AGGGCTCCTG AGAAGTCTGT GAGGCTGGTG
    GTAAATTACC TGAAGACACA GAAGGCAGTG GTTCGAGTCA GCCCGGACGT GCCCCTTCAC
    AACATCATCC CAGCTATTTG TGAAAAGTGT GAGGTCAGTC AGGAGCACAT TGTTCTTCTG
    CGAGACAGCA TCACTGGCGA GGAGCTGGAG CTTACCAAAT CCTTGGAGGA GCTGGGAATA
    AGCGAGCTGT ACGCCTGGGA CCGAAAAAGA GAACCAAGTC GAAGTGTTTC ACTAAGCAAC
    GATGCAATAG AAAAAGAAAA GAAAGGATTT CTGGGGTTTT TCAAAGCTAA TAGAAGCAAC
    AGCAAGACAG AAGAACACTT GAGGATGAAC AGAGATTATG GTGAGGAGGA TGTTTTTAGT
    TCTACATCTA CAAGTGAAGG AAGCTTGGAT GGTTTTTCCA CAGCACCAAA CTCCCCTTCG
    GTGAATTCCC GTTCCAGTAC CTTGGGTCCA TCGCTGTCCC TTGGTAATAT CTCAGGAATG
    ACAGCAAACC CAGAAGTGAA AAAGCGCAGA GCACCTCCTC CACCAGTTTT GACACCTCCG
    TTGCAGAATA TGGAGATGAG CTCCGCTCAG GAGAAGACAG CAGTACAGAT TTCACAAGGA
    GCATCTCTTA ACGATCTACA GAAAAAGAAG AGGCGTGCAC CTCTTCCACC AACTCAGTCT
    GCTCCACCTA CTCCAGCCAT GCCAAATAGG AGGGAAGAGA TGGAAGACAA GAGGAAGAGC
    ACAATGGGTG ATGGACGGCA GGTGCCACAA AAGCCTCCTA GGGGCCCCAC TCGTGACCCA
    CCCCAGTTAG TGATCCCCCC ACCCCCACCT TACCCTCCTC CTGAAAGAGA CAATATGGAT
    CCAGCAGTCT CTTACAGAGA AGCAGATGTT ACAGCACCAA CAAAACTGGT ACCTAAACAG
    AGCCTGCTGC CTGCACATGA TAATTTTTAT GTAGTGGATG ATACAGTTAT GGAGCTATCA
    GAGGTAGAAG AAACAGCGTC AGAAAGCAGC TGCTTTGCAT CTGAGGACAC TACCGAAGAC
    TCAGGTGTAG TGAGCTCCCC ATCTGACATT GTGTCACTGG ATTCACAAAA TGACAGTATG
    AAGTTGAGAG ACATCAAGCT GGTGAATGGC TATGTGGACT CAGACACGAT CTATGGTGCA
    GAGACTTGCT CTGTGAAGAA CGCGTACTGC GATGATATGG GATATGCCGG TACTCCACAG
    AGCACTAAAA ATGAAGAAAT GGCTGCGGCT AAGCATGAGA ATGAGGACAT ATTCATTGAT
    GCACAACTCC AGCAGACTTT GGCTGACCTG GATGAAGATT TAGAAGCTAT GGATGTTTTA
    AGTAACTCTG ACTCTGAGCA CATGAGTGAA GTGTTGAGCC CACATTCAGG AAAAGTTGAG
    GTCCCCCAAG ATGTTGCTAG TTCTGTTCCA GTAACAGTCA TAGATGATGC TCCAGAAGTT
    ACCATCTATA ACTCAGCTGG AAACCAAGAG ACAAGGGGTT CACAGAATCT ATCAGCTGAC
    TCTGTTAATA TGGGAAACTT CACAAATAGA AACAACAATG CAGGTTCCTT TGATAAGGGA
    CACACTGATG GTGGAGCTGT ATGTATACCC AAGGACCTAA TACATCAGCA GCCATCTGAT
    AATGAGTTCA GTCACATACC CGTGGAACAG AGGAGAGATA TGTTTGAATC TCTCACGTCC
    TCCTTTGAAA AAAGAAGTGA AAAGAATGTA AGAGTAGAAG CTCCGCCCAG ACACGTTATG
    AAGTCTGAGG AGTGTAAATC GAAGCTGACG TCATCTCACT CCAAGACTGC GACTGAAATC
    AATACAGCAA AAGAGAAAGT AAATCCATTT AATGAATGTA GTAAGAGGGA GAGACCTCTG
    AAAAAAAGTA AGTCAGAACT AGAAATCAAA AGGCCACCAG CAAAAAAGAT TGAAGTAGAA
    GAAGTTCCAT CAACACCTCC GTGGTGTCAT CGTGCCCAGA AATTGGAAAC AAGTTATGAA
    CCCAAAATGG GTTTGACGAC CTTCAAAGTT GTCCCTCCCA AACCTGAAGT AAAAAATTTT
    GATAGAGGGC TTTCAGTCTC CACTGGTGCT ATTAAGATTG ATGAACTAGG CAACCTTATC
    AACCCAAAGC CTGTTGTCAG TAAGAACATA CCAGGTACCT CTGCTGGTGA AAGTGAGGAA
    ATTCCTCTTG GGAAAGTGAA GGCATTCTGG AGGTCAAGTT CTATGGAAAA ACAAAGTGAT
    GACTCTGCTG AGCATCTTGC TAAAAAGCCA GCAGTTACCA CAAACTCTAA GTCCTTTGTT
    ACAAAGCAAG AATGCAAACC TGTCTGTCTT GCAGCTCCAA AACCTGTACC ACCACAAACT
    GTTATTTCCC AGGTAGGTGA AAAGCAGTCT GAGAATGAGA AGACCAAACC ACCTCTTCCA
    GTTACACAGT CTCAGGTAAA ACCATTAGTG GTCCCAGCCA TTAATAAGGA CAAGCCGGAG
    CTTCCATTTC TAAAGCCTCA CAGAAGAACT TCCAGTCAGT ATGTGGCCTC TGCCATTGCA
    AAACACATCA ATCTGCCTAC CTTTAAGACT GATTCTACAG ATTTGCATGA GAAAGATGAA
    AATAAGCACG GGGCAGGAGA GGTTAAAACT GAAGCTGAAG TTTCCCCCAA AAGGTGTATT
    ATTGTGGCCA AGTACAGCCC TGTGGAAACA GAATCAGCAG AAACAAGAGA AAAAATTTCA
    AGTATTTTTA CTTGTAGTCA AAAAGCATCC CACAGATTCA CACCTGGTGA TAATTCTGGC
    ATGGAAAACA AAGTAGTGAA CATCAGGGCA CCGAGTCAAG CAACTCCTGC TAGTTTCTAT
    AAAAAGAATG CCAGTGTCCC ACTTACTAAA TCCTCTTCAA AGGATAACAA CAAAGCAGAA
    GATGATCACA GTTTAAACAG CAAACAAGGT ACAGCAGAGA AAAAGATGCA TCTTTTGTTT
    GACCAGAAAT ACGAGACTTT GCCCCGTAGT AATTCAGCCT CCTCTCTCAA GTCACCTGAT
    CTCCAAGGAG CCTCATCTTC TTTCAGTTCA TCTTTGCGCA TCACTAAATG GCCTCCTGCT
    GACAGCGTGC AAGTTAGTTC CCTGAAAGCA TCGCCCGAGT TCAGTGGTGT GACAGCAAAG
    GCAGAATCAG AACAGAATGA CAAATCTGAT GATTCGGCTG CTAATGCTAT TGGCGAATTG
    GATGTTAATG TGCAGACTAA TATCTTCGGA CCAAAGAAGA AGTTCAAACC TGTTGTCCAA
    AAACCAATTC CGAAAGATAC ATCACTGCAC AGTGCCCTCA TGGAAGCTAT TCAAACAGGA
    GGGGGAAAAG AGAAACTCAG AAAGGTTTCA GACAGCGCGC TAAATGGCAA TCACAAGAAA
    CCATCATACA GTGAGCCAGA GAATGAGCGG TCGGCCCTAC TAGCGGCAAT TAGAGGCCAC
    AGTGGCACTT GCAAGCTGAA AAAAATCTCC TCATTGGCCT CCGAAGAGCT CCAGAGTTTT
    AGGAATGCAG AACTGTCTTC ACAGAAAGCA GAAGACCCTC AGGAAGAGCA GCTCTGCATT
    CCACCTGCCC CTGCCCAGCC ACCACCACCG CCTCCCCCTC AGCTGTCAAC AGCAACTTTC
    AAATTCTCTG CCACAACTAC GGGTGACCCT GGAGAGGCCA GGCGAGCCCT GATGGAGGCC
    ATTCGCTCTG GTGCTGGAGC AGCAAAGTTA AAAAAGGTCC CTCTGCTCGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32268
    Clone ID Related Accession (Same CDS sequence) XM_025147765.1 , XM_015282313.2 , XM_015282314.1 , XM_015282314.2 , XM_015282313.1
    Accession Version XM_025147765.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein cordon-bleu isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAAGCTC GTGCTCCTCC TCCACCAAAC CAACCTTCTG CAACCAGTAG AATTCACAGT 
    GAGCACAAGT CACCTGCAGA GACAGCCGTA ATTTCTGATC AGAGCCTTGT GAGCATGAAG
    GAGAATATGA TAAATAGAAT GGTGGACTTC ACAGTCATTT TACCCAGCGG GGTGGAACAG
    AAGAGCACAG TACAAGGAAG TAAAGCTGTG ATGGATCTTT TGGTTGATCT ATGCAGCCGG
    TATCACTTGA ATCCATCCCA GTACAGTCTT GAATTGAAGT CTTTGGAAAC TCAACAACCT
    TTGAGTTACA AGCCAAACAC TTTGATAGGA GCACTGGACG TACAGACAGT ACTCCTGAAA
    GAGAAGGTTC CTGAAGAGAA GATCAAGAGA CCTCTGCCGA GGGCTCCTGA GAAGTCTGTG
    AGGCTGGTGG TAAATTACCT GAAGACACAG AAGGCAGTGG TTCGAGTCAG CCCGGACGTG
    CCCCTTCACA ACATCATCCC AGCTATTTGT GAAAAGTGTG AGGTCAGTCA GGAGCACATT
    GTTCTTCTGC GAGACAGCAT CACTGGCGAG GAGCTGGAGC TTACCAAATC CTTGGAGGAG
    CTGGGAATAA GCGAGCTGTA CGCCTGGGAC CGAAAAAGAG TTCCTCCATC AAAAACTCAG
    TCTGACCCTT CTCTGAACTA CAGAGAACCA AGTCGAAGTG TTTCACTAAG CAACGATGCA
    ATAGAAAAAG AAAAGAAAGG ATTTCTGGGG TTTTTCAAAG CTAATAGAAG CAACAGCAAG
    ACAGAAGAAC ACTTGAGGAT GAACAGAGAT TATGGTGAGG AGGATGTTTT TAGTTCTACA
    TCTACAAGTG AAGGAAGCTT GGATGGTTTT TCCACAGCAC CAAACTCCCC TTCGGTGAAT
    TCCCGTTCCA GTACCTTGGG TCCATCGCTG TCCCTTGGTA ATATCTCAGG AATGACAGCA
    AACCCAGAAG TGAAAAAGCG CAGAGCACCT CCTCCACCAG TTTTGACACC TCCGTTGCAG
    AATATGGAGA TGAGCTCCGC TCAGGAGAAG ACAGCAGTAC AGATTTCACA AGGAGCATCT
    CTTAACGATC TACAGAAAAA GAAGAGGCGT GCACCTCTTC CACCAACTCA GTCTGCTCCA
    CCTACTCCAG CCATGCCAAA TAGGAGGGAA GAGATGGAAG ACAAGAGGAA GAGCACAATG
    GGTGATGGAC GGCAGGTGCC ACAAAAGCCT CCTAGGGGCC CCACTCGTGA CCCACCCCAG
    TTAGTGATCC CCCCACCCCC ACCTTACCCT CCTCCTGAAA GAGACAATAT GGATCCAGCA
    GTCTCTTACA GAGAAGCAGA TGTTACAGCA CCAACAAAAC TGGTACCTAA ACAGAGCCTG
    CTGCCTGCAC ATGATAATTT TTATGTAGTG GATGATACAG TTATGGAGCT ATCAGAGGTA
    GAAGAAACAG CGTCAGAAAG CAGCTGCTTT GCATCTGAGG ACACTACCGA AGACTCAGGT
    GTAGTGAGCT CCCCATCTGA CATTGTGTCA CTGGATTCAC AAAATGACAG TATGAAGTTG
    AGAGACATCA AGCTGGTGAA TGGCTATGTG GACTCAGACA CGATCTATGG TGCAGAGACT
    TGCTCTGTGA AGAACGCGTA CTGCGATGAT ATGGGATATG CCGGTACTCC ACAGAGCACT
    AAAAATGAAG AAATGGCTGC GGCTAAGCAT GAGAATGAGG ACATATTCAT TGATGCACAA
    CTCCAGCAGA CTTTGGCTGA CCTGGATGAA GATTTAGAAG CTATGGATGT TTTAAGTAAC
    TCTGACTCTG AGCACATGAG TGAAGTGTTG AGCCCACATT CAGGAAAAGT TGAGGTCCCC
    CAAGATGTTG CTAGTTCTGT TCCAGTAACA GTCATAGATG ATGCTCCAGA AGTTACCATC
    TATAACTCAG CTGGAAACCA AGAGACAAGG GGTTCACAGA ATCTATCAGC TGACTCTGTT
    AATATGGGAA ACTTCACAAA TAGAAACAAC AATGCAGGTT CCTTTGATAA GGGACACACT
    GATGGTGGAG CTGTATGTAT ACCCAAGGAC CTAATACATC AGCAGCCATC TGATAATGAG
    TTCAGTCACA TACCCGTGGA ACAGAGGAGA GATATGTTTG AATCTCTCAC GTCCTCCTTT
    GAAAAAAGAA GTGAAAAGAA TGTAAGAGTA GAAGCTCCGC CCAGACACGT TATGAAGTCT
    GAGGAGTGTA AATCGAAGCT GACGTCATCT CACTCCAAGA CTGCGACTGA AATCAATACA
    GCAAAAGAGA AAGTAAATCC ATTTAATGAA TGTAGTAAGA GGGAGAGACC TCTGAAAAAA
    AGTAAGTCAG AACTAGAAAT CAAAAGGCCA CCAGCAAAAA AGATTGAAGT AGAAGAAGTT
    CCATCAACAC CTCCGTGGTG TCATCGTGCC CAGAAATTGG AAACAAGTTA TGAACCCAAA
    ATGGGTTTGA CGACCTTCAA AGTTGTCCCT CCCAAACCTG AAGTAAAAAA TTTTGATAGA
    GGGCTTTCAG TCTCCACTGG TGCTATTAAG ATTGATGAAC TAGGCAACCT TATCAACCCA
    AAGCCTGTTG TCAGTAAGAA CATACCAGGT ACCTCTGCTG GTGAAAGTGA GGAAATTCCT
    CTTGGGAAAG TGAAGGCATT CTGGAGGTCA AGTTCTATGG AAAAACAAAG TGATGACTCT
    GCTGAGCATC TTGCTAAAAA GCCAGCAGTT ACCACAAACT CTAAGTCCTT TGTTACAAAG
    CAAGAATGCA AACCTGTCTG TCTTGCAGCT CCAAAACCTG TACCACCACA AACTGTTATT
    TCCCAGGTAG GTGAAAAGCA GTCTGAGAAT GAGAAGACCA AACCACCTCT TCCAGTTACA
    CAGTCTCAGG TAAAACCATT AGTGGTCCCA GCCATTAATA AGGACAAGCC GGAGCTTCCA
    TTTCTAAAGC CTCACAGAAG AACTTCCAGT CAGTATGTGG CCTCTGCCAT TGCAAAACAC
    ATCAATCTGC CTACCTTTAA GACTGATTCT ACAGATTTGC ATGAGAAAGA TGAAAATAAG
    CACGGGGCAG GAGAGGTTAA AACTGAAGCT GAAGTTTCCC CCAAAAGGTG TATTATTGTG
    GCCAAGTACA GCCCTGTGGA AACAGAATCA GCAGAAACAA GAGAAAAAAT TTCAAGTATT
    TTTACTTGTA GTCAAAAAGC ATCCCACAGA TTCACACCTG GTGATAATTC TGGCATGGAA
    AACAAAGTAG TGAACATCAG GGCACCGAGT CAAGCAACTC CTGCTAGTTT CTATAAAAAG
    AATGCCAGTG TCCCACTTAC TAAATCCTCT TCAAAGGATA ACAACAAAGC AGAAGATGAT
    CACAGTTTAA ACAGCAAACA AGGTACAGCA GAGAAAAAGA TGCATCTTTT GTTTGACCAG
    AAATACGAGA CTTTGCCCCG TAGTAATTCA GCCTCCTCTC TCAAGTCACC TGATCTCCAA
    GGAGCCTCAT CTTCTTTCAG TTCATCTTTG CGCATCACTA AATGGCCTCC TGCTGACAGC
    GTGCAAGTTA GTTCCCTGAA AGCATCGCCC GAGTTCAGTG GTGTGACAGC AAAGGCAGAA
    TCAGAACAGA ATGACAAATC TGATGATTCG GCTGCTAATG CTATTGGCGA ATTGGATGTT
    AATGTGCAGA CTAATATCTT CGGACCAAAG AAGAAGTTCA AACCTGTTGT CCAAAAACCA
    ATTCCGAAAG ATACATCACT GCACAGTGCC CTCATGGAAG CTATTCAAAC AGGAGGGGGA
    AAAGAGAAAC TCAGAAAGGT TTCAGACAGC GCGCTAAATG GCAATCACAA GAAACCATCA
    TACAGTGAGC CAGAGAATGA GCGGTCGGCC CTACTAGCGG CAATTAGAGG CCACAGTGGC
    ACTTGCAAGC TGAAAAAAAT CTCCTCATTG GCCTCCGAAG AGCTCCAGAG TTTTAGGAAT
    GCAGAACTGT CTTCACAGAA AGCAGAAGAC CCTCAGGAAG AGCAGCTCTG CATTCCACCT
    GCCCCTGCCC AGCCACCACC ACCGCCTCCC CCTCAGCTGT CAACAGCAAC TTTCAAATTC
    TCTGCCACAA CTACGGGTGA CCCTGGAGAG GCCAGGCGAG CCCTGATGGA GGCCATTCGC
    TCTGGTGCTG GAGCAGCAAA GTTAAAAAAG GTCCCTCTGC TCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025003533.1
    CDS124..4311
    Translation

    Target ORF information:

    RefSeq Version XM_025147765.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025147765.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAAGCTC GTGCTCCTCC TCCACCAAAC CAACCTTCTG CAACCAGTAG AATTCACAGT 
    GAGCACAAGT CACCTGCAGA GACAGCCGTA ATTTCTGATC AGAGCCTTGT GAGCATGAAG
    GAGAATATGA TAAATAGAAT GGTGGACTTC ACAGTCATTT TACCCAGCGG GGTGGAACAG
    AAGAGCACAG TACAAGGAAG TAAAGCTGTG ATGGATCTTT TGGTTGATCT ATGCAGCCGG
    TATCACTTGA ATCCATCCCA GTACAGTCTT GAATTGAAGT CTTTGGAAAC TCAACAACCT
    TTGAGTTACA AGCCAAACAC TTTGATAGGA GCACTGGACG TACAGACAGT ACTCCTGAAA
    GAGAAGGTTC CTGAAGAGAA GATCAAGAGA CCTCTGCCGA GGGCTCCTGA GAAGTCTGTG
    AGGCTGGTGG TAAATTACCT GAAGACACAG AAGGCAGTGG TTCGAGTCAG CCCGGACGTG
    CCCCTTCACA ACATCATCCC AGCTATTTGT GAAAAGTGTG AGGTCAGTCA GGAGCACATT
    GTTCTTCTGC GAGACAGCAT CACTGGCGAG GAGCTGGAGC TTACCAAATC CTTGGAGGAG
    CTGGGAATAA GCGAGCTGTA CGCCTGGGAC CGAAAAAGAG TTCCTCCATC AAAAACTCAG
    TCTGACCCTT CTCTGAACTA CAGAGAACCA AGTCGAAGTG TTTCACTAAG CAACGATGCA
    ATAGAAAAAG AAAAGAAAGG ATTTCTGGGG TTTTTCAAAG CTAATAGAAG CAACAGCAAG
    ACAGAAGAAC ACTTGAGGAT GAACAGAGAT TATGGTGAGG AGGATGTTTT TAGTTCTACA
    TCTACAAGTG AAGGAAGCTT GGATGGTTTT TCCACAGCAC CAAACTCCCC TTCGGTGAAT
    TCCCGTTCCA GTACCTTGGG TCCATCGCTG TCCCTTGGTA ATATCTCAGG AATGACAGCA
    AACCCAGAAG TGAAAAAGCG CAGAGCACCT CCTCCACCAG TTTTGACACC TCCGTTGCAG
    AATATGGAGA TGAGCTCCGC TCAGGAGAAG ACAGCAGTAC AGATTTCACA AGGAGCATCT
    CTTAACGATC TACAGAAAAA GAAGAGGCGT GCACCTCTTC CACCAACTCA GTCTGCTCCA
    CCTACTCCAG CCATGCCAAA TAGGAGGGAA GAGATGGAAG ACAAGAGGAA GAGCACAATG
    GGTGATGGAC GGCAGGTGCC ACAAAAGCCT CCTAGGGGCC CCACTCGTGA CCCACCCCAG
    TTAGTGATCC CCCCACCCCC ACCTTACCCT CCTCCTGAAA GAGACAATAT GGATCCAGCA
    GTCTCTTACA GAGAAGCAGA TGTTACAGCA CCAACAAAAC TGGTACCTAA ACAGAGCCTG
    CTGCCTGCAC ATGATAATTT TTATGTAGTG GATGATACAG TTATGGAGCT ATCAGAGGTA
    GAAGAAACAG CGTCAGAAAG CAGCTGCTTT GCATCTGAGG ACACTACCGA AGACTCAGGT
    GTAGTGAGCT CCCCATCTGA CATTGTGTCA CTGGATTCAC AAAATGACAG TATGAAGTTG
    AGAGACATCA AGCTGGTGAA TGGCTATGTG GACTCAGACA CGATCTATGG TGCAGAGACT
    TGCTCTGTGA AGAACGCGTA CTGCGATGAT ATGGGATATG CCGGTACTCC ACAGAGCACT
    AAAAATGAAG AAATGGCTGC GGCTAAGCAT GAGAATGAGG ACATATTCAT TGATGCACAA
    CTCCAGCAGA CTTTGGCTGA CCTGGATGAA GATTTAGAAG CTATGGATGT TTTAAGTAAC
    TCTGACTCTG AGCACATGAG TGAAGTGTTG AGCCCACATT CAGGAAAAGT TGAGGTCCCC
    CAAGATGTTG CTAGTTCTGT TCCAGTAACA GTCATAGATG ATGCTCCAGA AGTTACCATC
    TATAACTCAG CTGGAAACCA AGAGACAAGG GGTTCACAGA ATCTATCAGC TGACTCTGTT
    AATATGGGAA ACTTCACAAA TAGAAACAAC AATGCAGGTT CCTTTGATAA GGGACACACT
    GATGGTGGAG CTGTATGTAT ACCCAAGGAC CTAATACATC AGCAGCCATC TGATAATGAG
    TTCAGTCACA TACCCGTGGA ACAGAGGAGA GATATGTTTG AATCTCTCAC GTCCTCCTTT
    GAAAAAAGAA GTGAAAAGAA TGTAAGAGTA GAAGCTCCGC CCAGACACGT TATGAAGTCT
    GAGGAGTGTA AATCGAAGCT GACGTCATCT CACTCCAAGA CTGCGACTGA AATCAATACA
    GCAAAAGAGA AAGTAAATCC ATTTAATGAA TGTAGTAAGA GGGAGAGACC TCTGAAAAAA
    AGTAAGTCAG AACTAGAAAT CAAAAGGCCA CCAGCAAAAA AGATTGAAGT AGAAGAAGTT
    CCATCAACAC CTCCGTGGTG TCATCGTGCC CAGAAATTGG AAACAAGTTA TGAACCCAAA
    ATGGGTTTGA CGACCTTCAA AGTTGTCCCT CCCAAACCTG AAGTAAAAAA TTTTGATAGA
    GGGCTTTCAG TCTCCACTGG TGCTATTAAG ATTGATGAAC TAGGCAACCT TATCAACCCA
    AAGCCTGTTG TCAGTAAGAA CATACCAGGT ACCTCTGCTG GTGAAAGTGA GGAAATTCCT
    CTTGGGAAAG TGAAGGCATT CTGGAGGTCA AGTTCTATGG AAAAACAAAG TGATGACTCT
    GCTGAGCATC TTGCTAAAAA GCCAGCAGTT ACCACAAACT CTAAGTCCTT TGTTACAAAG
    CAAGAATGCA AACCTGTCTG TCTTGCAGCT CCAAAACCTG TACCACCACA AACTGTTATT
    TCCCAGGTAG GTGAAAAGCA GTCTGAGAAT GAGAAGACCA AACCACCTCT TCCAGTTACA
    CAGTCTCAGG TAAAACCATT AGTGGTCCCA GCCATTAATA AGGACAAGCC GGAGCTTCCA
    TTTCTAAAGC CTCACAGAAG AACTTCCAGT CAGTATGTGG CCTCTGCCAT TGCAAAACAC
    ATCAATCTGC CTACCTTTAA GACTGATTCT ACAGATTTGC ATGAGAAAGA TGAAAATAAG
    CACGGGGCAG GAGAGGTTAA AACTGAAGCT GAAGTTTCCC CCAAAAGGTG TATTATTGTG
    GCCAAGTACA GCCCTGTGGA AACAGAATCA GCAGAAACAA GAGAAAAAAT TTCAAGTATT
    TTTACTTGTA GTCAAAAAGC ATCCCACAGA TTCACACCTG GTGATAATTC TGGCATGGAA
    AACAAAGTAG TGAACATCAG GGCACCGAGT CAAGCAACTC CTGCTAGTTT CTATAAAAAG
    AATGCCAGTG TCCCACTTAC TAAATCCTCT TCAAAGGATA ACAACAAAGC AGAAGATGAT
    CACAGTTTAA ACAGCAAACA AGGTACAGCA GAGAAAAAGA TGCATCTTTT GTTTGACCAG
    AAATACGAGA CTTTGCCCCG TAGTAATTCA GCCTCCTCTC TCAAGTCACC TGATCTCCAA
    GGAGCCTCAT CTTCTTTCAG TTCATCTTTG CGCATCACTA AATGGCCTCC TGCTGACAGC
    GTGCAAGTTA GTTCCCTGAA AGCATCGCCC GAGTTCAGTG GTGTGACAGC AAAGGCAGAA
    TCAGAACAGA ATGACAAATC TGATGATTCG GCTGCTAATG CTATTGGCGA ATTGGATGTT
    AATGTGCAGA CTAATATCTT CGGACCAAAG AAGAAGTTCA AACCTGTTGT CCAAAAACCA
    ATTCCGAAAG ATACATCACT GCACAGTGCC CTCATGGAAG CTATTCAAAC AGGAGGGGGA
    AAAGAGAAAC TCAGAAAGGT TTCAGACAGC GCGCTAAATG GCAATCACAA GAAACCATCA
    TACAGTGAGC CAGAGAATGA GCGGTCGGCC CTACTAGCGG CAATTAGAGG CCACAGTGGC
    ACTTGCAAGC TGAAAAAAAT CTCCTCATTG GCCTCCGAAG AGCTCCAGAG TTTTAGGAAT
    GCAGAACTGT CTTCACAGAA AGCAGAAGAC CCTCAGGAAG AGCAGCTCTG CATTCCACCT
    GCCCCTGCCC AGCCACCACC ACCGCCTCCC CCTCAGCTGT CAACAGCAAC TTTCAAATTC
    TCTGCCACAA CTACGGGTGA CCCTGGAGAG GCCAGGCGAG CCCTGATGGA GGCCATTCGC
    TCTGGTGCTG GAGCAGCAAA GTTAAAAAAG GTCCCTCTGC TCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32268
    Clone ID Related Accession (Same CDS sequence) XM_025147765.1 , XM_015282313.2 , XM_015282314.1 , XM_015282314.2 , XM_015282313.1
    Accession Version XM_015282313.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein cordon-bleu isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015282313.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAAGCTC GTGCTCCTCC TCCACCAAAC CAACCTTCTG CAACCAGTAG AATTCACAGT 
    GAGCACAAGT CACCTGCAGA GACAGCCGTA ATTTCTGATC AGAGCCTTGT GAGCATGAAG
    GAGAATATGA TAAATAGAAT GGTGGACTTC ACAGTCATTT TACCCAGCGG GGTGGAACAG
    AAGAGCACAG TACAAGGAAG TAAAGCTGTG ATGGATCTTT TGGTTGATCT ATGCAGCCGG
    TATCACTTGA ATCCATCCCA GTACAGTCTT GAATTGAAGT CTTTGGAAAC TCAACAACCT
    TTGAGTTACA AGCCAAACAC TTTGATAGGA GCACTGGACG TACAGACAGT ACTCCTGAAA
    GAGAAGGTTC CTGAAGAGAA GATCAAGAGA CCTCTGCCGA GGGCTCCTGA GAAGTCTGTG
    AGGCTGGTGG TAAATTACCT GAAGACACAG AAGGCAGTGG TTCGAGTCAG CCCGGACGTG
    CCCCTTCACA ACATCATCCC AGCTATTTGT GAAAAGTGTG AGGTCAGTCA GGAGCACATT
    GTTCTTCTGC GAGACAGCAT CACTGGCGAG GAGCTGGAGC TTACCAAATC CTTGGAGGAG
    CTGGGAATAA GCGAGCTGTA CGCCTGGGAC CGAAAAAGAG TTCCTCCATC AAAAACTCAG
    TCTGACCCTT CTCTGAACTA CAGAGAACCA AGTCGAAGTG TTTCACTAAG CAACGATGCA
    ATAGAAAAAG AAAAGAAAGG ATTTCTGGGG TTTTTCAAAG CTAATAGAAG CAACAGCAAG
    ACAGAAGAAC ACTTGAGGAT GAACAGAGAT TATGGTGAGG AGGATGTTTT TAGTTCTACA
    TCTACAAGTG AAGGAAGCTT GGATGGTTTT TCCACAGCAC CAAACTCCCC TTCGGTGAAT
    TCCCGTTCCA GTACCTTGGG TCCATCGCTG TCCCTTGGTA ATATCTCAGG AATGACAGCA
    AACCCAGAAG TGAAAAAGCG CAGAGCACCT CCTCCACCAG TTTTGACACC TCCGTTGCAG
    AATATGGAGA TGAGCTCCGC TCAGGAGAAG ACAGCAGTAC AGATTTCACA AGGAGCATCT
    CTTAACGATC TACAGAAAAA GAAGAGGCGT GCACCTCTTC CACCAACTCA GTCTGCTCCA
    CCTACTCCAG CCATGCCAAA TAGGAGGGAA GAGATGGAAG ACAAGAGGAA GAGCACAATG
    GGTGATGGAC GGCAGGTGCC ACAAAAGCCT CCTAGGGGCC CCACTCGTGA CCCACCCCAG
    TTAGTGATCC CCCCACCCCC ACCTTACCCT CCTCCTGAAA GAGACAATAT GGATCCAGCA
    GTCTCTTACA GAGAAGCAGA TGTTACAGCA CCAACAAAAC TGGTACCTAA ACAGAGCCTG
    CTGCCTGCAC ATGATAATTT TTATGTAGTG GATGATACAG TTATGGAGCT ATCAGAGGTA
    GAAGAAACAG CGTCAGAAAG CAGCTGCTTT GCATCTGAGG ACACTACCGA AGACTCAGGT
    GTAGTGAGCT CCCCATCTGA CATTGTGTCA CTGGATTCAC AAAATGACAG TATGAAGTTG
    AGAGACATCA AGCTGGTGAA TGGCTATGTG GACTCAGACA CGATCTATGG TGCAGAGACT
    TGCTCTGTGA AGAACGCGTA CTGCGATGAT ATGGGATATG CCGGTACTCC ACAGAGCACT
    AAAAATGAAG AAATGGCTGC GGCTAAGCAT GAGAATGAGG ACATATTCAT TGATGCACAA
    CTCCAGCAGA CTTTGGCTGA CCTGGATGAA GATTTAGAAG CTATGGATGT TTTAAGTAAC
    TCTGACTCTG AGCACATGAG TGAAGTGTTG AGCCCACATT CAGGAAAAGT TGAGGTCCCC
    CAAGATGTTG CTAGTTCTGT TCCAGTAACA GTCATAGATG ATGCTCCAGA AGTTACCATC
    TATAACTCAG CTGGAAACCA AGAGACAAGG GGTTCACAGA ATCTATCAGC TGACTCTGTT
    AATATGGGAA ACTTCACAAA TAGAAACAAC AATGCAGGTT CCTTTGATAA GGGACACACT
    GATGGTGGAG CTGTATGTAT ACCCAAGGAC CTAATACATC AGCAGCCATC TGATAATGAG
    TTCAGTCACA TACCCGTGGA ACAGAGGAGA GATATGTTTG AATCTCTCAC GTCCTCCTTT
    GAAAAAAGAA GTGAAAAGAA TGTAAGAGTA GAAGCTCCGC CCAGACACGT TATGAAGTCT
    GAGGAGTGTA AATCGAAGCT GACGTCATCT CACTCCAAGA CTGCGACTGA AATCAATACA
    GCAAAAGAGA AAGTAAATCC ATTTAATGAA TGTAGTAAGA GGGAGAGACC TCTGAAAAAA
    AGTAAGTCAG AACTAGAAAT CAAAAGGCCA CCAGCAAAAA AGATTGAAGT AGAAGAAGTT
    CCATCAACAC CTCCGTGGTG TCATCGTGCC CAGAAATTGG AAACAAGTTA TGAACCCAAA
    ATGGGTTTGA CGACCTTCAA AGTTGTCCCT CCCAAACCTG AAGTAAAAAA TTTTGATAGA
    GGGCTTTCAG TCTCCACTGG TGCTATTAAG ATTGATGAAC TAGGCAACCT TATCAACCCA
    AAGCCTGTTG TCAGTAAGAA CATACCAGGT ACCTCTGCTG GTGAAAGTGA GGAAATTCCT
    CTTGGGAAAG TGAAGGCATT CTGGAGGTCA AGTTCTATGG AAAAACAAAG TGATGACTCT
    GCTGAGCATC TTGCTAAAAA GCCAGCAGTT ACCACAAACT CTAAGTCCTT TGTTACAAAG
    CAAGAATGCA AACCTGTCTG TCTTGCAGCT CCAAAACCTG TACCACCACA AACTGTTATT
    TCCCAGGTAG GTGAAAAGCA GTCTGAGAAT GAGAAGACCA AACCACCTCT TCCAGTTACA
    CAGTCTCAGG TAAAACCATT AGTGGTCCCA GCCATTAATA AGGACAAGCC GGAGCTTCCA
    TTTCTAAAGC CTCACAGAAG AACTTCCAGT CAGTATGTGG CCTCTGCCAT TGCAAAACAC
    ATCAATCTGC CTACCTTTAA GACTGATTCT ACAGATTTGC ATGAGAAAGA TGAAAATAAG
    CACGGGGCAG GAGAGGTTAA AACTGAAGCT GAAGTTTCCC CCAAAAGGTG TATTATTGTG
    GCCAAGTACA GCCCTGTGGA AACAGAATCA GCAGAAACAA GAGAAAAAAT TTCAAGTATT
    TTTACTTGTA GTCAAAAAGC ATCCCACAGA TTCACACCTG GTGATAATTC TGGCATGGAA
    AACAAAGTAG TGAACATCAG GGCACCGAGT CAAGCAACTC CTGCTAGTTT CTATAAAAAG
    AATGCCAGTG TCCCACTTAC TAAATCCTCT TCAAAGGATA ACAACAAAGC AGAAGATGAT
    CACAGTTTAA ACAGCAAACA AGGTACAGCA GAGAAAAAGA TGCATCTTTT GTTTGACCAG
    AAATACGAGA CTTTGCCCCG TAGTAATTCA GCCTCCTCTC TCAAGTCACC TGATCTCCAA
    GGAGCCTCAT CTTCTTTCAG TTCATCTTTG CGCATCACTA AATGGCCTCC TGCTGACAGC
    GTGCAAGTTA GTTCCCTGAA AGCATCGCCC GAGTTCAGTG GTGTGACAGC AAAGGCAGAA
    TCAGAACAGA ATGACAAATC TGATGATTCG GCTGCTAATG CTATTGGCGA ATTGGATGTT
    AATGTGCAGA CTAATATCTT CGGACCAAAG AAGAAGTTCA AACCTGTTGT CCAAAAACCA
    ATTCCGAAAG ATACATCACT GCACAGTGCC CTCATGGAAG CTATTCAAAC AGGAGGGGGA
    AAAGAGAAAC TCAGAAAGGT TTCAGACAGC GCGCTAAATG GCAATCACAA GAAACCATCA
    TACAGTGAGC CAGAGAATGA GCGGTCGGCC CTACTAGCGG CAATTAGAGG CCACAGTGGC
    ACTTGCAAGC TGAAAAAAAT CTCCTCATTG GCCTCCGAAG AGCTCCAGAG TTTTAGGAAT
    GCAGAACTGT CTTCACAGAA AGCAGAAGAC CCTCAGGAAG AGCAGCTCTG CATTCCACCT
    GCCCCTGCCC AGCCACCACC ACCGCCTCCC CCTCAGCTGT CAACAGCAAC TTTCAAATTC
    TCTGCCACAA CTACGGGTGA CCCTGGAGAG GCCAGGCGAG CCCTGATGGA GGCCATTCGC
    TCTGGTGCTG GAGCAGCAAA GTTAAAAAAG GTCCCTCTGC TCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137799.1
    CDS84..4271
    Translation

    Target ORF information:

    RefSeq Version XM_015282313.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282313.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAAGCTC GTGCTCCTCC TCCACCAAAC CAACCTTCTG CAACCAGTAG AATTCACAGT 
    GAGCACAAGT CACCTGCAGA GACAGCCGTA ATTTCTGATC AGAGCCTTGT GAGCATGAAG
    GAGAATATGA TAAATAGAAT GGTGGACTTC ACAGTCATTT TACCCAGCGG GGTGGAACAG
    AAGAGCACAG TACAAGGAAG TAAAGCTGTG ATGGATCTTT TGGTTGATCT ATGCAGCCGG
    TATCACTTGA ATCCATCCCA GTACAGTCTT GAATTGAAGT CTTTGGAAAC TCAACAACCT
    TTGAGTTACA AGCCAAACAC TTTGATAGGA GCACTGGACG TACAGACAGT ACTCCTGAAA
    GAGAAGGTTC CTGAAGAGAA GATCAAGAGA CCTCTGCCGA GGGCTCCTGA GAAGTCTGTG
    AGGCTGGTGG TAAATTACCT GAAGACACAG AAGGCAGTGG TTCGAGTCAG CCCGGACGTG
    CCCCTTCACA ACATCATCCC AGCTATTTGT GAAAAGTGTG AGGTCAGTCA GGAGCACATT
    GTTCTTCTGC GAGACAGCAT CACTGGCGAG GAGCTGGAGC TTACCAAATC CTTGGAGGAG
    CTGGGAATAA GCGAGCTGTA CGCCTGGGAC CGAAAAAGAG TTCCTCCATC AAAAACTCAG
    TCTGACCCTT CTCTGAACTA CAGAGAACCA AGTCGAAGTG TTTCACTAAG CAACGATGCA
    ATAGAAAAAG AAAAGAAAGG ATTTCTGGGG TTTTTCAAAG CTAATAGAAG CAACAGCAAG
    ACAGAAGAAC ACTTGAGGAT GAACAGAGAT TATGGTGAGG AGGATGTTTT TAGTTCTACA
    TCTACAAGTG AAGGAAGCTT GGATGGTTTT TCCACAGCAC CAAACTCCCC TTCGGTGAAT
    TCCCGTTCCA GTACCTTGGG TCCATCGCTG TCCCTTGGTA ATATCTCAGG AATGACAGCA
    AACCCAGAAG TGAAAAAGCG CAGAGCACCT CCTCCACCAG TTTTGACACC TCCGTTGCAG
    AATATGGAGA TGAGCTCCGC TCAGGAGAAG ACAGCAGTAC AGATTTCACA AGGAGCATCT
    CTTAACGATC TACAGAAAAA GAAGAGGCGT GCACCTCTTC CACCAACTCA GTCTGCTCCA
    CCTACTCCAG CCATGCCAAA TAGGAGGGAA GAGATGGAAG ACAAGAGGAA GAGCACAATG
    GGTGATGGAC GGCAGGTGCC ACAAAAGCCT CCTAGGGGCC CCACTCGTGA CCCACCCCAG
    TTAGTGATCC CCCCACCCCC ACCTTACCCT CCTCCTGAAA GAGACAATAT GGATCCAGCA
    GTCTCTTACA GAGAAGCAGA TGTTACAGCA CCAACAAAAC TGGTACCTAA ACAGAGCCTG
    CTGCCTGCAC ATGATAATTT TTATGTAGTG GATGATACAG TTATGGAGCT ATCAGAGGTA
    GAAGAAACAG CGTCAGAAAG CAGCTGCTTT GCATCTGAGG ACACTACCGA AGACTCAGGT
    GTAGTGAGCT CCCCATCTGA CATTGTGTCA CTGGATTCAC AAAATGACAG TATGAAGTTG
    AGAGACATCA AGCTGGTGAA TGGCTATGTG GACTCAGACA CGATCTATGG TGCAGAGACT
    TGCTCTGTGA AGAACGCGTA CTGCGATGAT ATGGGATATG CCGGTACTCC ACAGAGCACT
    AAAAATGAAG AAATGGCTGC GGCTAAGCAT GAGAATGAGG ACATATTCAT TGATGCACAA
    CTCCAGCAGA CTTTGGCTGA CCTGGATGAA GATTTAGAAG CTATGGATGT TTTAAGTAAC
    TCTGACTCTG AGCACATGAG TGAAGTGTTG AGCCCACATT CAGGAAAAGT TGAGGTCCCC
    CAAGATGTTG CTAGTTCTGT TCCAGTAACA GTCATAGATG ATGCTCCAGA AGTTACCATC
    TATAACTCAG CTGGAAACCA AGAGACAAGG GGTTCACAGA ATCTATCAGC TGACTCTGTT
    AATATGGGAA ACTTCACAAA TAGAAACAAC AATGCAGGTT CCTTTGATAA GGGACACACT
    GATGGTGGAG CTGTATGTAT ACCCAAGGAC CTAATACATC AGCAGCCATC TGATAATGAG
    TTCAGTCACA TACCCGTGGA ACAGAGGAGA GATATGTTTG AATCTCTCAC GTCCTCCTTT
    GAAAAAAGAA GTGAAAAGAA TGTAAGAGTA GAAGCTCCGC CCAGACACGT TATGAAGTCT
    GAGGAGTGTA AATCGAAGCT GACGTCATCT CACTCCAAGA CTGCGACTGA AATCAATACA
    GCAAAAGAGA AAGTAAATCC ATTTAATGAA TGTAGTAAGA GGGAGAGACC TCTGAAAAAA
    AGTAAGTCAG AACTAGAAAT CAAAAGGCCA CCAGCAAAAA AGATTGAAGT AGAAGAAGTT
    CCATCAACAC CTCCGTGGTG TCATCGTGCC CAGAAATTGG AAACAAGTTA TGAACCCAAA
    ATGGGTTTGA CGACCTTCAA AGTTGTCCCT CCCAAACCTG AAGTAAAAAA TTTTGATAGA
    GGGCTTTCAG TCTCCACTGG TGCTATTAAG ATTGATGAAC TAGGCAACCT TATCAACCCA
    AAGCCTGTTG TCAGTAAGAA CATACCAGGT ACCTCTGCTG GTGAAAGTGA GGAAATTCCT
    CTTGGGAAAG TGAAGGCATT CTGGAGGTCA AGTTCTATGG AAAAACAAAG TGATGACTCT
    GCTGAGCATC TTGCTAAAAA GCCAGCAGTT ACCACAAACT CTAAGTCCTT TGTTACAAAG
    CAAGAATGCA AACCTGTCTG TCTTGCAGCT CCAAAACCTG TACCACCACA AACTGTTATT
    TCCCAGGTAG GTGAAAAGCA GTCTGAGAAT GAGAAGACCA AACCACCTCT TCCAGTTACA
    CAGTCTCAGG TAAAACCATT AGTGGTCCCA GCCATTAATA AGGACAAGCC GGAGCTTCCA
    TTTCTAAAGC CTCACAGAAG AACTTCCAGT CAGTATGTGG CCTCTGCCAT TGCAAAACAC
    ATCAATCTGC CTACCTTTAA GACTGATTCT ACAGATTTGC ATGAGAAAGA TGAAAATAAG
    CACGGGGCAG GAGAGGTTAA AACTGAAGCT GAAGTTTCCC CCAAAAGGTG TATTATTGTG
    GCCAAGTACA GCCCTGTGGA AACAGAATCA GCAGAAACAA GAGAAAAAAT TTCAAGTATT
    TTTACTTGTA GTCAAAAAGC ATCCCACAGA TTCACACCTG GTGATAATTC TGGCATGGAA
    AACAAAGTAG TGAACATCAG GGCACCGAGT CAAGCAACTC CTGCTAGTTT CTATAAAAAG
    AATGCCAGTG TCCCACTTAC TAAATCCTCT TCAAAGGATA ACAACAAAGC AGAAGATGAT
    CACAGTTTAA ACAGCAAACA AGGTACAGCA GAGAAAAAGA TGCATCTTTT GTTTGACCAG
    AAATACGAGA CTTTGCCCCG TAGTAATTCA GCCTCCTCTC TCAAGTCACC TGATCTCCAA
    GGAGCCTCAT CTTCTTTCAG TTCATCTTTG CGCATCACTA AATGGCCTCC TGCTGACAGC
    GTGCAAGTTA GTTCCCTGAA AGCATCGCCC GAGTTCAGTG GTGTGACAGC AAAGGCAGAA
    TCAGAACAGA ATGACAAATC TGATGATTCG GCTGCTAATG CTATTGGCGA ATTGGATGTT
    AATGTGCAGA CTAATATCTT CGGACCAAAG AAGAAGTTCA AACCTGTTGT CCAAAAACCA
    ATTCCGAAAG ATACATCACT GCACAGTGCC CTCATGGAAG CTATTCAAAC AGGAGGGGGA
    AAAGAGAAAC TCAGAAAGGT TTCAGACAGC GCGCTAAATG GCAATCACAA GAAACCATCA
    TACAGTGAGC CAGAGAATGA GCGGTCGGCC CTACTAGCGG CAATTAGAGG CCACAGTGGC
    ACTTGCAAGC TGAAAAAAAT CTCCTCATTG GCCTCCGAAG AGCTCCAGAG TTTTAGGAAT
    GCAGAACTGT CTTCACAGAA AGCAGAAGAC CCTCAGGAAG AGCAGCTCTG CATTCCACCT
    GCCCCTGCCC AGCCACCACC ACCGCCTCCC CCTCAGCTGT CAACAGCAAC TTTCAAATTC
    TCTGCCACAA CTACGGGTGA CCCTGGAGAG GCCAGGCGAG CCCTGATGGA GGCCATTCGC
    TCTGGTGCTG GAGCAGCAAA GTTAAAAAAG GTCCCTCTGC TCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32268
    Clone ID Related Accession (Same CDS sequence) XM_025147765.1 , XM_015282313.2 , XM_015282314.1 , XM_015282314.2 , XM_015282313.1
    Accession Version XM_015282314.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product protein cordon-bleu isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455789.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAAGCTC GTGCTCCTCC TCCACCAAAC CAACCTTCTG CAACCAGTAG AATTCACAGT 
    GAGCACAAGT CACCTGCAGA GACAGCCGTA ATTTCTGATC AGAGCCTTGT GAGCATGAAG
    GAGAATATGA TAAATAGAAT GGTGGACTTC ACAGTCATTT TACCCAGCGG GGTGGAACAG
    AAGAGCACAG TACAAGGAAG TAAAGCTGTG ATGGATCTTT TGGTTGATCT ATGCAGCCGG
    TATCACTTGA ATCCATCCCA GTACAGTCTT GAATTGAAGT CTTTGGAAAC TCAACAACCT
    TTGAGTTACA AGCCAAACAC TTTGATAGGA GCACTGGACG TACAGACAGT ACTCCTGAAA
    GAGAAGGTTC CTGAAGAGAA GATCAAGAGA CCTCTGCCGA GGGCTCCTGA GAAGTCTGTG
    AGGCTGGTGG TAAATTACCT GAAGACACAG AAGGCAGTGG TTCGAGTCAG CCCGGACGTG
    CCCCTTCACA ACATCATCCC AGCTATTTGT GAAAAGTGTG AGGTCAGTCA GGAGCACATT
    GTTCTTCTGC GAGACAGCAT CACTGGCGAG GAGCTGGAGC TTACCAAATC CTTGGAGGAG
    CTGGGAATAA GCGAGCTGTA CGCCTGGGAC CGAAAAAGAG TTCCTCCATC AAAAACTCAG
    TCTGACCCTT CTCTGAACTA CAGAGAACCA AGTCGAAGTG TTTCACTAAG CAACGATGCA
    ATAGAAAAAG AAAAGAAAGG ATTTCTGGGG TTTTTCAAAG CTAATAGAAG CAACAGCAAG
    ACAGAAGAAC ACTTGAGGAT GAACAGAGAT TATGGTGAGG AGGATGTTTT TAGTTCTACA
    TCTACAAGTG AAGGAAGCTT GGATGGTTTT TCCACAGCAC CAAACTCCCC TTCGGTGAAT
    TCCCGTTCCA GTACCTTGGG TCCATCGCTG TCCCTTGGTA ATATCTCAGG AATGACAGCA
    AACCCAGAAG TGAAAAAGCG CAGAGCACCT CCTCCACCAG TTTTGACACC TCCGTTGCAG
    AATATGGAGA TGAGCTCCGC TCAGGAGAAG ACAGCAGTAC AGATTTCACA AGGAGCATCT
    CTTAACGATC TACAGAAAAA GAAGAGGCGT GCACCTCTTC CACCAACTCA GTCTGCTCCA
    CCTACTCCAG CCATGCCAAA TAGGAGGGAA GAGATGGAAG ACAAGAGGAA GAGCACAATG
    GGTGATGGAC GGCAGGTGCC ACAAAAGCCT CCTAGGGGCC CCACTCGTGA CCCACCCCAG
    TTAGTGATCC CCCCACCCCC ACCTTACCCT CCTCCTGAAA GAGACAATAT GGATCCAGCA
    GTCTCTTACA GAGAAGCAGA TGTTACAGCA CCAACAAAAC TGGTACCTAA ACAGAGCCTG
    CTGCCTGCAC ATGATAATTT TTATGTAGTG GATGATACAG TTATGGAGCT ATCAGAGGTA
    GAAGAAACAG CGTCAGAAAG CAGCTGCTTT GCATCTGAGG ACACTACCGA AGACTCAGGT
    GTAGTGAGCT CCCCATCTGA CATTGTGTCA CTGGATTCAC AAAATGACAG TATGAAGTTG
    AGAGACATCA AGCTGGTGAA TGGCTATGTG GACTCAGACA CGATCTATGG TGCAGAGACT
    TGCTCTGTGA AGAACGCGTA CTGCGATGAT ATGGGATATG CCGGTACTCC ACAGAGCACT
    AAAAATGAAG AAATGGCTGC GGCTAAGCAT GAGAATGAGG ACATATTCAT TGATGCACAA
    CTCCAGCAGA CTTTGGCTGA CCTGGATGAA GATTTAGAAG CTATGGATGT TTTAAGTAAC
    TCTGACTCTG AGCACATGAG TGAAGTGTTG AGCCCACATT CAGGAAAAGT TGAGGTCCCC
    CAAGATGTTG CTAGTTCTGT TCCAGTAACA GTCATAGATG ATGCTCCAGA AGTTACCATC
    TATAACTCAG CTGGAAACCA AGAGACAAGG GGTTCACAGA ATCTATCAGC TGACTCTGTT
    AATATGGGAA ACTTCACAAA TAGAAACAAC AATGCAGGTT CCTTTGATAA GGGACACACT
    GATGGTGGAG CTGTATGTAT ACCCAAGGAC CTAATACATC AGCAGCCATC TGATAATGAG
    TTCAGTCACA TACCCGTGGA ACAGAGGAGA GATATGTTTG AATCTCTCAC GTCCTCCTTT
    GAAAAAAGAA GTGAAAAGAA TGTAAGAGTA GAAGCTCCGC CCAGACACGT TATGAAGTCT
    GAGGAGTGTA AATCGAAGCT GACGTCATCT CACTCCAAGA CTGCGACTGA AATCAATACA
    GCAAAAGAGA AAGTAAATCC ATTTAATGAA TGTAGTAAGA GGGAGAGACC TCTGAAAAAA
    AGTAAGTCAG AACTAGAAAT CAAAAGGCCA CCAGCAAAAA AGATTGAAGT AGAAGAAGTT
    CCATCAACAC CTCCGTGGTG TCATCGTGCC CAGAAATTGG AAACAAGTTA TGAACCCAAA
    ATGGGTTTGA CGACCTTCAA AGTTGTCCCT CCCAAACCTG AAGTAAAAAA TTTTGATAGA
    GGGCTTTCAG TCTCCACTGG TGCTATTAAG ATTGATGAAC TAGGCAACCT TATCAACCCA
    AAGCCTGTTG TCAGTAAGAA CATACCAGGT ACCTCTGCTG GTGAAAGTGA GGAAATTCCT
    CTTGGGAAAG TGAAGGCATT CTGGAGGTCA AGTTCTATGG AAAAACAAAG TGATGACTCT
    GCTGAGCATC TTGCTAAAAA GCCAGCAGTT ACCACAAACT CTAAGTCCTT TGTTACAAAG
    CAAGAATGCA AACCTGTCTG TCTTGCAGCT CCAAAACCTG TACCACCACA AACTGTTATT
    TCCCAGGTAG GTGAAAAGCA GTCTGAGAAT GAGAAGACCA AACCACCTCT TCCAGTTACA
    CAGTCTCAGG TAAAACCATT AGTGGTCCCA GCCATTAATA AGGACAAGCC GGAGCTTCCA
    TTTCTAAAGC CTCACAGAAG AACTTCCAGT CAGTATGTGG CCTCTGCCAT TGCAAAACAC
    ATCAATCTGC CTACCTTTAA GACTGATTCT ACAGATTTGC ATGAGAAAGA TGAAAATAAG
    CACGGGGCAG GAGAGGTTAA AACTGAAGCT GAAGTTTCCC CCAAAAGGTG TATTATTGTG
    GCCAAGTACA GCCCTGTGGA AACAGAATCA GCAGAAACAA GAGAAAAAAT TTCAAGTATT
    TTTACTTGTA GTCAAAAAGC ATCCCACAGA TTCACACCTG GTGATAATTC TGGCATGGAA
    AACAAAGTAG TGAACATCAG GGCACCGAGT CAAGCAACTC CTGCTAGTTT CTATAAAAAG
    AATGCCAGTG TCCCACTTAC TAAATCCTCT TCAAAGGATA ACAACAAAGC AGAAGATGAT
    CACAGTTTAA ACAGCAAACA AGGTACAGCA GAGAAAAAGA TGCATCTTTT GTTTGACCAG
    AAATACGAGA CTTTGCCCCG TAGTAATTCA GCCTCCTCTC TCAAGTCACC TGATCTCCAA
    GGAGCCTCAT CTTCTTTCAG TTCATCTTTG CGCATCACTA AATGGCCTCC TGCTGACAGC
    GTGCAAGTTA GTTCCCTGAA AGCATCGCCC GAGTTCAGTG GTGTGACAGC AAAGGCAGAA
    TCAGAACAGA ATGACAAATC TGATGATTCG GCTGCTAATG CTATTGGCGA ATTGGATGTT
    AATGTGCAGA CTAATATCTT CGGACCAAAG AAGAAGTTCA AACCTGTTGT CCAAAAACCA
    ATTCCGAAAG ATACATCACT GCACAGTGCC CTCATGGAAG CTATTCAAAC AGGAGGGGGA
    AAAGAGAAAC TCAGAAAGGT TTCAGACAGC GCGCTAAATG GCAATCACAA GAAACCATCA
    TACAGTGAGC CAGAGAATGA GCGGTCGGCC CTACTAGCGG CAATTAGAGG CCACAGTGGC
    ACTTGCAAGC TGAAAAAAAT CTCCTCATTG GCCTCCGAAG AGCTCCAGAG TTTTAGGAAT
    GCAGAACTGT CTTCACAGAA AGCAGAAGAC CCTCAGGAAG AGCAGCTCTG CATTCCACCT
    GCCCCTGCCC AGCCACCACC ACCGCCTCCC CCTCAGCTGT CAACAGCAAC TTTCAAATTC
    TCTGCCACAA CTACGGGTGA CCCTGGAGAG GCCAGGCGAG CCCTGATGGA GGCCATTCGC
    TCTGGTGCTG GAGCAGCAAA GTTAAAAAAG GTCCCTCTGC TCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137800.1
    CDS73..4260
    Translation

    Target ORF information:

    RefSeq Version XM_015282314.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282314.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAAGCTC GTGCTCCTCC TCCACCAAAC CAACCTTCTG CAACCAGTAG AATTCACAGT 
    GAGCACAAGT CACCTGCAGA GACAGCCGTA ATTTCTGATC AGAGCCTTGT GAGCATGAAG
    GAGAATATGA TAAATAGAAT GGTGGACTTC ACAGTCATTT TACCCAGCGG GGTGGAACAG
    AAGAGCACAG TACAAGGAAG TAAAGCTGTG ATGGATCTTT TGGTTGATCT ATGCAGCCGG
    TATCACTTGA ATCCATCCCA GTACAGTCTT GAATTGAAGT CTTTGGAAAC TCAACAACCT
    TTGAGTTACA AGCCAAACAC TTTGATAGGA GCACTGGACG TACAGACAGT ACTCCTGAAA
    GAGAAGGTTC CTGAAGAGAA GATCAAGAGA CCTCTGCCGA GGGCTCCTGA GAAGTCTGTG
    AGGCTGGTGG TAAATTACCT GAAGACACAG AAGGCAGTGG TTCGAGTCAG CCCGGACGTG
    CCCCTTCACA ACATCATCCC AGCTATTTGT GAAAAGTGTG AGGTCAGTCA GGAGCACATT
    GTTCTTCTGC GAGACAGCAT CACTGGCGAG GAGCTGGAGC TTACCAAATC CTTGGAGGAG
    CTGGGAATAA GCGAGCTGTA CGCCTGGGAC CGAAAAAGAG TTCCTCCATC AAAAACTCAG
    TCTGACCCTT CTCTGAACTA CAGAGAACCA AGTCGAAGTG TTTCACTAAG CAACGATGCA
    ATAGAAAAAG AAAAGAAAGG ATTTCTGGGG TTTTTCAAAG CTAATAGAAG CAACAGCAAG
    ACAGAAGAAC ACTTGAGGAT GAACAGAGAT TATGGTGAGG AGGATGTTTT TAGTTCTACA
    TCTACAAGTG AAGGAAGCTT GGATGGTTTT TCCACAGCAC CAAACTCCCC TTCGGTGAAT
    TCCCGTTCCA GTACCTTGGG TCCATCGCTG TCCCTTGGTA ATATCTCAGG AATGACAGCA
    AACCCAGAAG TGAAAAAGCG CAGAGCACCT CCTCCACCAG TTTTGACACC TCCGTTGCAG
    AATATGGAGA TGAGCTCCGC TCAGGAGAAG ACAGCAGTAC AGATTTCACA AGGAGCATCT
    CTTAACGATC TACAGAAAAA GAAGAGGCGT GCACCTCTTC CACCAACTCA GTCTGCTCCA
    CCTACTCCAG CCATGCCAAA TAGGAGGGAA GAGATGGAAG ACAAGAGGAA GAGCACAATG
    GGTGATGGAC GGCAGGTGCC ACAAAAGCCT CCTAGGGGCC CCACTCGTGA CCCACCCCAG
    TTAGTGATCC CCCCACCCCC ACCTTACCCT CCTCCTGAAA GAGACAATAT GGATCCAGCA
    GTCTCTTACA GAGAAGCAGA TGTTACAGCA CCAACAAAAC TGGTACCTAA ACAGAGCCTG
    CTGCCTGCAC ATGATAATTT TTATGTAGTG GATGATACAG TTATGGAGCT ATCAGAGGTA
    GAAGAAACAG CGTCAGAAAG CAGCTGCTTT GCATCTGAGG ACACTACCGA AGACTCAGGT
    GTAGTGAGCT CCCCATCTGA CATTGTGTCA CTGGATTCAC AAAATGACAG TATGAAGTTG
    AGAGACATCA AGCTGGTGAA TGGCTATGTG GACTCAGACA CGATCTATGG TGCAGAGACT
    TGCTCTGTGA AGAACGCGTA CTGCGATGAT ATGGGATATG CCGGTACTCC ACAGAGCACT
    AAAAATGAAG AAATGGCTGC GGCTAAGCAT GAGAATGAGG ACATATTCAT TGATGCACAA
    CTCCAGCAGA CTTTGGCTGA CCTGGATGAA GATTTAGAAG CTATGGATGT TTTAAGTAAC
    TCTGACTCTG AGCACATGAG TGAAGTGTTG AGCCCACATT CAGGAAAAGT TGAGGTCCCC
    CAAGATGTTG CTAGTTCTGT TCCAGTAACA GTCATAGATG ATGCTCCAGA AGTTACCATC
    TATAACTCAG CTGGAAACCA AGAGACAAGG GGTTCACAGA ATCTATCAGC TGACTCTGTT
    AATATGGGAA ACTTCACAAA TAGAAACAAC AATGCAGGTT CCTTTGATAA GGGACACACT
    GATGGTGGAG CTGTATGTAT ACCCAAGGAC CTAATACATC AGCAGCCATC TGATAATGAG
    TTCAGTCACA TACCCGTGGA ACAGAGGAGA GATATGTTTG AATCTCTCAC GTCCTCCTTT
    GAAAAAAGAA GTGAAAAGAA TGTAAGAGTA GAAGCTCCGC CCAGACACGT TATGAAGTCT
    GAGGAGTGTA AATCGAAGCT GACGTCATCT CACTCCAAGA CTGCGACTGA AATCAATACA
    GCAAAAGAGA AAGTAAATCC ATTTAATGAA TGTAGTAAGA GGGAGAGACC TCTGAAAAAA
    AGTAAGTCAG AACTAGAAAT CAAAAGGCCA CCAGCAAAAA AGATTGAAGT AGAAGAAGTT
    CCATCAACAC CTCCGTGGTG TCATCGTGCC CAGAAATTGG AAACAAGTTA TGAACCCAAA
    ATGGGTTTGA CGACCTTCAA AGTTGTCCCT CCCAAACCTG AAGTAAAAAA TTTTGATAGA
    GGGCTTTCAG TCTCCACTGG TGCTATTAAG ATTGATGAAC TAGGCAACCT TATCAACCCA
    AAGCCTGTTG TCAGTAAGAA CATACCAGGT ACCTCTGCTG GTGAAAGTGA GGAAATTCCT
    CTTGGGAAAG TGAAGGCATT CTGGAGGTCA AGTTCTATGG AAAAACAAAG TGATGACTCT
    GCTGAGCATC TTGCTAAAAA GCCAGCAGTT ACCACAAACT CTAAGTCCTT TGTTACAAAG
    CAAGAATGCA AACCTGTCTG TCTTGCAGCT CCAAAACCTG TACCACCACA AACTGTTATT
    TCCCAGGTAG GTGAAAAGCA GTCTGAGAAT GAGAAGACCA AACCACCTCT TCCAGTTACA
    CAGTCTCAGG TAAAACCATT AGTGGTCCCA GCCATTAATA AGGACAAGCC GGAGCTTCCA
    TTTCTAAAGC CTCACAGAAG AACTTCCAGT CAGTATGTGG CCTCTGCCAT TGCAAAACAC
    ATCAATCTGC CTACCTTTAA GACTGATTCT ACAGATTTGC ATGAGAAAGA TGAAAATAAG
    CACGGGGCAG GAGAGGTTAA AACTGAAGCT GAAGTTTCCC CCAAAAGGTG TATTATTGTG
    GCCAAGTACA GCCCTGTGGA AACAGAATCA GCAGAAACAA GAGAAAAAAT TTCAAGTATT
    TTTACTTGTA GTCAAAAAGC ATCCCACAGA TTCACACCTG GTGATAATTC TGGCATGGAA
    AACAAAGTAG TGAACATCAG GGCACCGAGT CAAGCAACTC CTGCTAGTTT CTATAAAAAG
    AATGCCAGTG TCCCACTTAC TAAATCCTCT TCAAAGGATA ACAACAAAGC AGAAGATGAT
    CACAGTTTAA ACAGCAAACA AGGTACAGCA GAGAAAAAGA TGCATCTTTT GTTTGACCAG
    AAATACGAGA CTTTGCCCCG TAGTAATTCA GCCTCCTCTC TCAAGTCACC TGATCTCCAA
    GGAGCCTCAT CTTCTTTCAG TTCATCTTTG CGCATCACTA AATGGCCTCC TGCTGACAGC
    GTGCAAGTTA GTTCCCTGAA AGCATCGCCC GAGTTCAGTG GTGTGACAGC AAAGGCAGAA
    TCAGAACAGA ATGACAAATC TGATGATTCG GCTGCTAATG CTATTGGCGA ATTGGATGTT
    AATGTGCAGA CTAATATCTT CGGACCAAAG AAGAAGTTCA AACCTGTTGT CCAAAAACCA
    ATTCCGAAAG ATACATCACT GCACAGTGCC CTCATGGAAG CTATTCAAAC AGGAGGGGGA
    AAAGAGAAAC TCAGAAAGGT TTCAGACAGC GCGCTAAATG GCAATCACAA GAAACCATCA
    TACAGTGAGC CAGAGAATGA GCGGTCGGCC CTACTAGCGG CAATTAGAGG CCACAGTGGC
    ACTTGCAAGC TGAAAAAAAT CTCCTCATTG GCCTCCGAAG AGCTCCAGAG TTTTAGGAAT
    GCAGAACTGT CTTCACAGAA AGCAGAAGAC CCTCAGGAAG AGCAGCTCTG CATTCCACCT
    GCCCCTGCCC AGCCACCACC ACCGCCTCCC CCTCAGCTGT CAACAGCAAC TTTCAAATTC
    TCTGCCACAA CTACGGGTGA CCCTGGAGAG GCCAGGCGAG CCCTGATGGA GGCCATTCGC
    TCTGGTGCTG GAGCAGCAAA GTTAAAAAAG GTCCCTCTGC TCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32268
    Clone ID Related Accession (Same CDS sequence) XM_025147765.1 , XM_015282313.2 , XM_015282314.1 , XM_015282314.2 , XM_015282313.1
    Accession Version XM_015282314.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein cordon-bleu isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015282314.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAAGCTC GTGCTCCTCC TCCACCAAAC CAACCTTCTG CAACCAGTAG AATTCACAGT 
    GAGCACAAGT CACCTGCAGA GACAGCCGTA ATTTCTGATC AGAGCCTTGT GAGCATGAAG
    GAGAATATGA TAAATAGAAT GGTGGACTTC ACAGTCATTT TACCCAGCGG GGTGGAACAG
    AAGAGCACAG TACAAGGAAG TAAAGCTGTG ATGGATCTTT TGGTTGATCT ATGCAGCCGG
    TATCACTTGA ATCCATCCCA GTACAGTCTT GAATTGAAGT CTTTGGAAAC TCAACAACCT
    TTGAGTTACA AGCCAAACAC TTTGATAGGA GCACTGGACG TACAGACAGT ACTCCTGAAA
    GAGAAGGTTC CTGAAGAGAA GATCAAGAGA CCTCTGCCGA GGGCTCCTGA GAAGTCTGTG
    AGGCTGGTGG TAAATTACCT GAAGACACAG AAGGCAGTGG TTCGAGTCAG CCCGGACGTG
    CCCCTTCACA ACATCATCCC AGCTATTTGT GAAAAGTGTG AGGTCAGTCA GGAGCACATT
    GTTCTTCTGC GAGACAGCAT CACTGGCGAG GAGCTGGAGC TTACCAAATC CTTGGAGGAG
    CTGGGAATAA GCGAGCTGTA CGCCTGGGAC CGAAAAAGAG TTCCTCCATC AAAAACTCAG
    TCTGACCCTT CTCTGAACTA CAGAGAACCA AGTCGAAGTG TTTCACTAAG CAACGATGCA
    ATAGAAAAAG AAAAGAAAGG ATTTCTGGGG TTTTTCAAAG CTAATAGAAG CAACAGCAAG
    ACAGAAGAAC ACTTGAGGAT GAACAGAGAT TATGGTGAGG AGGATGTTTT TAGTTCTACA
    TCTACAAGTG AAGGAAGCTT GGATGGTTTT TCCACAGCAC CAAACTCCCC TTCGGTGAAT
    TCCCGTTCCA GTACCTTGGG TCCATCGCTG TCCCTTGGTA ATATCTCAGG AATGACAGCA
    AACCCAGAAG TGAAAAAGCG CAGAGCACCT CCTCCACCAG TTTTGACACC TCCGTTGCAG
    AATATGGAGA TGAGCTCCGC TCAGGAGAAG ACAGCAGTAC AGATTTCACA AGGAGCATCT
    CTTAACGATC TACAGAAAAA GAAGAGGCGT GCACCTCTTC CACCAACTCA GTCTGCTCCA
    CCTACTCCAG CCATGCCAAA TAGGAGGGAA GAGATGGAAG ACAAGAGGAA GAGCACAATG
    GGTGATGGAC GGCAGGTGCC ACAAAAGCCT CCTAGGGGCC CCACTCGTGA CCCACCCCAG
    TTAGTGATCC CCCCACCCCC ACCTTACCCT CCTCCTGAAA GAGACAATAT GGATCCAGCA
    GTCTCTTACA GAGAAGCAGA TGTTACAGCA CCAACAAAAC TGGTACCTAA ACAGAGCCTG
    CTGCCTGCAC ATGATAATTT TTATGTAGTG GATGATACAG TTATGGAGCT ATCAGAGGTA
    GAAGAAACAG CGTCAGAAAG CAGCTGCTTT GCATCTGAGG ACACTACCGA AGACTCAGGT
    GTAGTGAGCT CCCCATCTGA CATTGTGTCA CTGGATTCAC AAAATGACAG TATGAAGTTG
    AGAGACATCA AGCTGGTGAA TGGCTATGTG GACTCAGACA CGATCTATGG TGCAGAGACT
    TGCTCTGTGA AGAACGCGTA CTGCGATGAT ATGGGATATG CCGGTACTCC ACAGAGCACT
    AAAAATGAAG AAATGGCTGC GGCTAAGCAT GAGAATGAGG ACATATTCAT TGATGCACAA
    CTCCAGCAGA CTTTGGCTGA CCTGGATGAA GATTTAGAAG CTATGGATGT TTTAAGTAAC
    TCTGACTCTG AGCACATGAG TGAAGTGTTG AGCCCACATT CAGGAAAAGT TGAGGTCCCC
    CAAGATGTTG CTAGTTCTGT TCCAGTAACA GTCATAGATG ATGCTCCAGA AGTTACCATC
    TATAACTCAG CTGGAAACCA AGAGACAAGG GGTTCACAGA ATCTATCAGC TGACTCTGTT
    AATATGGGAA ACTTCACAAA TAGAAACAAC AATGCAGGTT CCTTTGATAA GGGACACACT
    GATGGTGGAG CTGTATGTAT ACCCAAGGAC CTAATACATC AGCAGCCATC TGATAATGAG
    TTCAGTCACA TACCCGTGGA ACAGAGGAGA GATATGTTTG AATCTCTCAC GTCCTCCTTT
    GAAAAAAGAA GTGAAAAGAA TGTAAGAGTA GAAGCTCCGC CCAGACACGT TATGAAGTCT
    GAGGAGTGTA AATCGAAGCT GACGTCATCT CACTCCAAGA CTGCGACTGA AATCAATACA
    GCAAAAGAGA AAGTAAATCC ATTTAATGAA TGTAGTAAGA GGGAGAGACC TCTGAAAAAA
    AGTAAGTCAG AACTAGAAAT CAAAAGGCCA CCAGCAAAAA AGATTGAAGT AGAAGAAGTT
    CCATCAACAC CTCCGTGGTG TCATCGTGCC CAGAAATTGG AAACAAGTTA TGAACCCAAA
    ATGGGTTTGA CGACCTTCAA AGTTGTCCCT CCCAAACCTG AAGTAAAAAA TTTTGATAGA
    GGGCTTTCAG TCTCCACTGG TGCTATTAAG ATTGATGAAC TAGGCAACCT TATCAACCCA
    AAGCCTGTTG TCAGTAAGAA CATACCAGGT ACCTCTGCTG GTGAAAGTGA GGAAATTCCT
    CTTGGGAAAG TGAAGGCATT CTGGAGGTCA AGTTCTATGG AAAAACAAAG TGATGACTCT
    GCTGAGCATC TTGCTAAAAA GCCAGCAGTT ACCACAAACT CTAAGTCCTT TGTTACAAAG
    CAAGAATGCA AACCTGTCTG TCTTGCAGCT CCAAAACCTG TACCACCACA AACTGTTATT
    TCCCAGGTAG GTGAAAAGCA GTCTGAGAAT GAGAAGACCA AACCACCTCT TCCAGTTACA
    CAGTCTCAGG TAAAACCATT AGTGGTCCCA GCCATTAATA AGGACAAGCC GGAGCTTCCA
    TTTCTAAAGC CTCACAGAAG AACTTCCAGT CAGTATGTGG CCTCTGCCAT TGCAAAACAC
    ATCAATCTGC CTACCTTTAA GACTGATTCT ACAGATTTGC ATGAGAAAGA TGAAAATAAG
    CACGGGGCAG GAGAGGTTAA AACTGAAGCT GAAGTTTCCC CCAAAAGGTG TATTATTGTG
    GCCAAGTACA GCCCTGTGGA AACAGAATCA GCAGAAACAA GAGAAAAAAT TTCAAGTATT
    TTTACTTGTA GTCAAAAAGC ATCCCACAGA TTCACACCTG GTGATAATTC TGGCATGGAA
    AACAAAGTAG TGAACATCAG GGCACCGAGT CAAGCAACTC CTGCTAGTTT CTATAAAAAG
    AATGCCAGTG TCCCACTTAC TAAATCCTCT TCAAAGGATA ACAACAAAGC AGAAGATGAT
    CACAGTTTAA ACAGCAAACA AGGTACAGCA GAGAAAAAGA TGCATCTTTT GTTTGACCAG
    AAATACGAGA CTTTGCCCCG TAGTAATTCA GCCTCCTCTC TCAAGTCACC TGATCTCCAA
    GGAGCCTCAT CTTCTTTCAG TTCATCTTTG CGCATCACTA AATGGCCTCC TGCTGACAGC
    GTGCAAGTTA GTTCCCTGAA AGCATCGCCC GAGTTCAGTG GTGTGACAGC AAAGGCAGAA
    TCAGAACAGA ATGACAAATC TGATGATTCG GCTGCTAATG CTATTGGCGA ATTGGATGTT
    AATGTGCAGA CTAATATCTT CGGACCAAAG AAGAAGTTCA AACCTGTTGT CCAAAAACCA
    ATTCCGAAAG ATACATCACT GCACAGTGCC CTCATGGAAG CTATTCAAAC AGGAGGGGGA
    AAAGAGAAAC TCAGAAAGGT TTCAGACAGC GCGCTAAATG GCAATCACAA GAAACCATCA
    TACAGTGAGC CAGAGAATGA GCGGTCGGCC CTACTAGCGG CAATTAGAGG CCACAGTGGC
    ACTTGCAAGC TGAAAAAAAT CTCCTCATTG GCCTCCGAAG AGCTCCAGAG TTTTAGGAAT
    GCAGAACTGT CTTCACAGAA AGCAGAAGAC CCTCAGGAAG AGCAGCTCTG CATTCCACCT
    GCCCCTGCCC AGCCACCACC ACCGCCTCCC CCTCAGCTGT CAACAGCAAC TTTCAAATTC
    TCTGCCACAA CTACGGGTGA CCCTGGAGAG GCCAGGCGAG CCCTGATGGA GGCCATTCGC
    TCTGGTGCTG GAGCAGCAAA GTTAAAAAAG GTCCCTCTGC TCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137800.1
    CDS30..4217
    Translation

    Target ORF information:

    RefSeq Version XM_015282314.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282314.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAAGCTC GTGCTCCTCC TCCACCAAAC CAACCTTCTG CAACCAGTAG AATTCACAGT 
    GAGCACAAGT CACCTGCAGA GACAGCCGTA ATTTCTGATC AGAGCCTTGT GAGCATGAAG
    GAGAATATGA TAAATAGAAT GGTGGACTTC ACAGTCATTT TACCCAGCGG GGTGGAACAG
    AAGAGCACAG TACAAGGAAG TAAAGCTGTG ATGGATCTTT TGGTTGATCT ATGCAGCCGG
    TATCACTTGA ATCCATCCCA GTACAGTCTT GAATTGAAGT CTTTGGAAAC TCAACAACCT
    TTGAGTTACA AGCCAAACAC TTTGATAGGA GCACTGGACG TACAGACAGT ACTCCTGAAA
    GAGAAGGTTC CTGAAGAGAA GATCAAGAGA CCTCTGCCGA GGGCTCCTGA GAAGTCTGTG
    AGGCTGGTGG TAAATTACCT GAAGACACAG AAGGCAGTGG TTCGAGTCAG CCCGGACGTG
    CCCCTTCACA ACATCATCCC AGCTATTTGT GAAAAGTGTG AGGTCAGTCA GGAGCACATT
    GTTCTTCTGC GAGACAGCAT CACTGGCGAG GAGCTGGAGC TTACCAAATC CTTGGAGGAG
    CTGGGAATAA GCGAGCTGTA CGCCTGGGAC CGAAAAAGAG TTCCTCCATC AAAAACTCAG
    TCTGACCCTT CTCTGAACTA CAGAGAACCA AGTCGAAGTG TTTCACTAAG CAACGATGCA
    ATAGAAAAAG AAAAGAAAGG ATTTCTGGGG TTTTTCAAAG CTAATAGAAG CAACAGCAAG
    ACAGAAGAAC ACTTGAGGAT GAACAGAGAT TATGGTGAGG AGGATGTTTT TAGTTCTACA
    TCTACAAGTG AAGGAAGCTT GGATGGTTTT TCCACAGCAC CAAACTCCCC TTCGGTGAAT
    TCCCGTTCCA GTACCTTGGG TCCATCGCTG TCCCTTGGTA ATATCTCAGG AATGACAGCA
    AACCCAGAAG TGAAAAAGCG CAGAGCACCT CCTCCACCAG TTTTGACACC TCCGTTGCAG
    AATATGGAGA TGAGCTCCGC TCAGGAGAAG ACAGCAGTAC AGATTTCACA AGGAGCATCT
    CTTAACGATC TACAGAAAAA GAAGAGGCGT GCACCTCTTC CACCAACTCA GTCTGCTCCA
    CCTACTCCAG CCATGCCAAA TAGGAGGGAA GAGATGGAAG ACAAGAGGAA GAGCACAATG
    GGTGATGGAC GGCAGGTGCC ACAAAAGCCT CCTAGGGGCC CCACTCGTGA CCCACCCCAG
    TTAGTGATCC CCCCACCCCC ACCTTACCCT CCTCCTGAAA GAGACAATAT GGATCCAGCA
    GTCTCTTACA GAGAAGCAGA TGTTACAGCA CCAACAAAAC TGGTACCTAA ACAGAGCCTG
    CTGCCTGCAC ATGATAATTT TTATGTAGTG GATGATACAG TTATGGAGCT ATCAGAGGTA
    GAAGAAACAG CGTCAGAAAG CAGCTGCTTT GCATCTGAGG ACACTACCGA AGACTCAGGT
    GTAGTGAGCT CCCCATCTGA CATTGTGTCA CTGGATTCAC AAAATGACAG TATGAAGTTG
    AGAGACATCA AGCTGGTGAA TGGCTATGTG GACTCAGACA CGATCTATGG TGCAGAGACT
    TGCTCTGTGA AGAACGCGTA CTGCGATGAT ATGGGATATG CCGGTACTCC ACAGAGCACT
    AAAAATGAAG AAATGGCTGC GGCTAAGCAT GAGAATGAGG ACATATTCAT TGATGCACAA
    CTCCAGCAGA CTTTGGCTGA CCTGGATGAA GATTTAGAAG CTATGGATGT TTTAAGTAAC
    TCTGACTCTG AGCACATGAG TGAAGTGTTG AGCCCACATT CAGGAAAAGT TGAGGTCCCC
    CAAGATGTTG CTAGTTCTGT TCCAGTAACA GTCATAGATG ATGCTCCAGA AGTTACCATC
    TATAACTCAG CTGGAAACCA AGAGACAAGG GGTTCACAGA ATCTATCAGC TGACTCTGTT
    AATATGGGAA ACTTCACAAA TAGAAACAAC AATGCAGGTT CCTTTGATAA GGGACACACT
    GATGGTGGAG CTGTATGTAT ACCCAAGGAC CTAATACATC AGCAGCCATC TGATAATGAG
    TTCAGTCACA TACCCGTGGA ACAGAGGAGA GATATGTTTG AATCTCTCAC GTCCTCCTTT
    GAAAAAAGAA GTGAAAAGAA TGTAAGAGTA GAAGCTCCGC CCAGACACGT TATGAAGTCT
    GAGGAGTGTA AATCGAAGCT GACGTCATCT CACTCCAAGA CTGCGACTGA AATCAATACA
    GCAAAAGAGA AAGTAAATCC ATTTAATGAA TGTAGTAAGA GGGAGAGACC TCTGAAAAAA
    AGTAAGTCAG AACTAGAAAT CAAAAGGCCA CCAGCAAAAA AGATTGAAGT AGAAGAAGTT
    CCATCAACAC CTCCGTGGTG TCATCGTGCC CAGAAATTGG AAACAAGTTA TGAACCCAAA
    ATGGGTTTGA CGACCTTCAA AGTTGTCCCT CCCAAACCTG AAGTAAAAAA TTTTGATAGA
    GGGCTTTCAG TCTCCACTGG TGCTATTAAG ATTGATGAAC TAGGCAACCT TATCAACCCA
    AAGCCTGTTG TCAGTAAGAA CATACCAGGT ACCTCTGCTG GTGAAAGTGA GGAAATTCCT
    CTTGGGAAAG TGAAGGCATT CTGGAGGTCA AGTTCTATGG AAAAACAAAG TGATGACTCT
    GCTGAGCATC TTGCTAAAAA GCCAGCAGTT ACCACAAACT CTAAGTCCTT TGTTACAAAG
    CAAGAATGCA AACCTGTCTG TCTTGCAGCT CCAAAACCTG TACCACCACA AACTGTTATT
    TCCCAGGTAG GTGAAAAGCA GTCTGAGAAT GAGAAGACCA AACCACCTCT TCCAGTTACA
    CAGTCTCAGG TAAAACCATT AGTGGTCCCA GCCATTAATA AGGACAAGCC GGAGCTTCCA
    TTTCTAAAGC CTCACAGAAG AACTTCCAGT CAGTATGTGG CCTCTGCCAT TGCAAAACAC
    ATCAATCTGC CTACCTTTAA GACTGATTCT ACAGATTTGC ATGAGAAAGA TGAAAATAAG
    CACGGGGCAG GAGAGGTTAA AACTGAAGCT GAAGTTTCCC CCAAAAGGTG TATTATTGTG
    GCCAAGTACA GCCCTGTGGA AACAGAATCA GCAGAAACAA GAGAAAAAAT TTCAAGTATT
    TTTACTTGTA GTCAAAAAGC ATCCCACAGA TTCACACCTG GTGATAATTC TGGCATGGAA
    AACAAAGTAG TGAACATCAG GGCACCGAGT CAAGCAACTC CTGCTAGTTT CTATAAAAAG
    AATGCCAGTG TCCCACTTAC TAAATCCTCT TCAAAGGATA ACAACAAAGC AGAAGATGAT
    CACAGTTTAA ACAGCAAACA AGGTACAGCA GAGAAAAAGA TGCATCTTTT GTTTGACCAG
    AAATACGAGA CTTTGCCCCG TAGTAATTCA GCCTCCTCTC TCAAGTCACC TGATCTCCAA
    GGAGCCTCAT CTTCTTTCAG TTCATCTTTG CGCATCACTA AATGGCCTCC TGCTGACAGC
    GTGCAAGTTA GTTCCCTGAA AGCATCGCCC GAGTTCAGTG GTGTGACAGC AAAGGCAGAA
    TCAGAACAGA ATGACAAATC TGATGATTCG GCTGCTAATG CTATTGGCGA ATTGGATGTT
    AATGTGCAGA CTAATATCTT CGGACCAAAG AAGAAGTTCA AACCTGTTGT CCAAAAACCA
    ATTCCGAAAG ATACATCACT GCACAGTGCC CTCATGGAAG CTATTCAAAC AGGAGGGGGA
    AAAGAGAAAC TCAGAAAGGT TTCAGACAGC GCGCTAAATG GCAATCACAA GAAACCATCA
    TACAGTGAGC CAGAGAATGA GCGGTCGGCC CTACTAGCGG CAATTAGAGG CCACAGTGGC
    ACTTGCAAGC TGAAAAAAAT CTCCTCATTG GCCTCCGAAG AGCTCCAGAG TTTTAGGAAT
    GCAGAACTGT CTTCACAGAA AGCAGAAGAC CCTCAGGAAG AGCAGCTCTG CATTCCACCT
    GCCCCTGCCC AGCCACCACC ACCGCCTCCC CCTCAGCTGT CAACAGCAAC TTTCAAATTC
    TCTGCCACAA CTACGGGTGA CCCTGGAGAG GCCAGGCGAG CCCTGATGGA GGCCATTCGC
    TCTGGTGCTG GAGCAGCAAA GTTAAAAAAG GTCCCTCTGC TCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32268
    Clone ID Related Accession (Same CDS sequence) XM_025147765.1 , XM_015282313.2 , XM_015282314.1 , XM_015282314.2 , XM_015282313.1
    Accession Version XM_015282313.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product protein cordon-bleu isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455789.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAAGCTC GTGCTCCTCC TCCACCAAAC CAACCTTCTG CAACCAGTAG AATTCACAGT 
    GAGCACAAGT CACCTGCAGA GACAGCCGTA ATTTCTGATC AGAGCCTTGT GAGCATGAAG
    GAGAATATGA TAAATAGAAT GGTGGACTTC ACAGTCATTT TACCCAGCGG GGTGGAACAG
    AAGAGCACAG TACAAGGAAG TAAAGCTGTG ATGGATCTTT TGGTTGATCT ATGCAGCCGG
    TATCACTTGA ATCCATCCCA GTACAGTCTT GAATTGAAGT CTTTGGAAAC TCAACAACCT
    TTGAGTTACA AGCCAAACAC TTTGATAGGA GCACTGGACG TACAGACAGT ACTCCTGAAA
    GAGAAGGTTC CTGAAGAGAA GATCAAGAGA CCTCTGCCGA GGGCTCCTGA GAAGTCTGTG
    AGGCTGGTGG TAAATTACCT GAAGACACAG AAGGCAGTGG TTCGAGTCAG CCCGGACGTG
    CCCCTTCACA ACATCATCCC AGCTATTTGT GAAAAGTGTG AGGTCAGTCA GGAGCACATT
    GTTCTTCTGC GAGACAGCAT CACTGGCGAG GAGCTGGAGC TTACCAAATC CTTGGAGGAG
    CTGGGAATAA GCGAGCTGTA CGCCTGGGAC CGAAAAAGAG TTCCTCCATC AAAAACTCAG
    TCTGACCCTT CTCTGAACTA CAGAGAACCA AGTCGAAGTG TTTCACTAAG CAACGATGCA
    ATAGAAAAAG AAAAGAAAGG ATTTCTGGGG TTTTTCAAAG CTAATAGAAG CAACAGCAAG
    ACAGAAGAAC ACTTGAGGAT GAACAGAGAT TATGGTGAGG AGGATGTTTT TAGTTCTACA
    TCTACAAGTG AAGGAAGCTT GGATGGTTTT TCCACAGCAC CAAACTCCCC TTCGGTGAAT
    TCCCGTTCCA GTACCTTGGG TCCATCGCTG TCCCTTGGTA ATATCTCAGG AATGACAGCA
    AACCCAGAAG TGAAAAAGCG CAGAGCACCT CCTCCACCAG TTTTGACACC TCCGTTGCAG
    AATATGGAGA TGAGCTCCGC TCAGGAGAAG ACAGCAGTAC AGATTTCACA AGGAGCATCT
    CTTAACGATC TACAGAAAAA GAAGAGGCGT GCACCTCTTC CACCAACTCA GTCTGCTCCA
    CCTACTCCAG CCATGCCAAA TAGGAGGGAA GAGATGGAAG ACAAGAGGAA GAGCACAATG
    GGTGATGGAC GGCAGGTGCC ACAAAAGCCT CCTAGGGGCC CCACTCGTGA CCCACCCCAG
    TTAGTGATCC CCCCACCCCC ACCTTACCCT CCTCCTGAAA GAGACAATAT GGATCCAGCA
    GTCTCTTACA GAGAAGCAGA TGTTACAGCA CCAACAAAAC TGGTACCTAA ACAGAGCCTG
    CTGCCTGCAC ATGATAATTT TTATGTAGTG GATGATACAG TTATGGAGCT ATCAGAGGTA
    GAAGAAACAG CGTCAGAAAG CAGCTGCTTT GCATCTGAGG ACACTACCGA AGACTCAGGT
    GTAGTGAGCT CCCCATCTGA CATTGTGTCA CTGGATTCAC AAAATGACAG TATGAAGTTG
    AGAGACATCA AGCTGGTGAA TGGCTATGTG GACTCAGACA CGATCTATGG TGCAGAGACT
    TGCTCTGTGA AGAACGCGTA CTGCGATGAT ATGGGATATG CCGGTACTCC ACAGAGCACT
    AAAAATGAAG AAATGGCTGC GGCTAAGCAT GAGAATGAGG ACATATTCAT TGATGCACAA
    CTCCAGCAGA CTTTGGCTGA CCTGGATGAA GATTTAGAAG CTATGGATGT TTTAAGTAAC
    TCTGACTCTG AGCACATGAG TGAAGTGTTG AGCCCACATT CAGGAAAAGT TGAGGTCCCC
    CAAGATGTTG CTAGTTCTGT TCCAGTAACA GTCATAGATG ATGCTCCAGA AGTTACCATC
    TATAACTCAG CTGGAAACCA AGAGACAAGG GGTTCACAGA ATCTATCAGC TGACTCTGTT
    AATATGGGAA ACTTCACAAA TAGAAACAAC AATGCAGGTT CCTTTGATAA GGGACACACT
    GATGGTGGAG CTGTATGTAT ACCCAAGGAC CTAATACATC AGCAGCCATC TGATAATGAG
    TTCAGTCACA TACCCGTGGA ACAGAGGAGA GATATGTTTG AATCTCTCAC GTCCTCCTTT
    GAAAAAAGAA GTGAAAAGAA TGTAAGAGTA GAAGCTCCGC CCAGACACGT TATGAAGTCT
    GAGGAGTGTA AATCGAAGCT GACGTCATCT CACTCCAAGA CTGCGACTGA AATCAATACA
    GCAAAAGAGA AAGTAAATCC ATTTAATGAA TGTAGTAAGA GGGAGAGACC TCTGAAAAAA
    AGTAAGTCAG AACTAGAAAT CAAAAGGCCA CCAGCAAAAA AGATTGAAGT AGAAGAAGTT
    CCATCAACAC CTCCGTGGTG TCATCGTGCC CAGAAATTGG AAACAAGTTA TGAACCCAAA
    ATGGGTTTGA CGACCTTCAA AGTTGTCCCT CCCAAACCTG AAGTAAAAAA TTTTGATAGA
    GGGCTTTCAG TCTCCACTGG TGCTATTAAG ATTGATGAAC TAGGCAACCT TATCAACCCA
    AAGCCTGTTG TCAGTAAGAA CATACCAGGT ACCTCTGCTG GTGAAAGTGA GGAAATTCCT
    CTTGGGAAAG TGAAGGCATT CTGGAGGTCA AGTTCTATGG AAAAACAAAG TGATGACTCT
    GCTGAGCATC TTGCTAAAAA GCCAGCAGTT ACCACAAACT CTAAGTCCTT TGTTACAAAG
    CAAGAATGCA AACCTGTCTG TCTTGCAGCT CCAAAACCTG TACCACCACA AACTGTTATT
    TCCCAGGTAG GTGAAAAGCA GTCTGAGAAT GAGAAGACCA AACCACCTCT TCCAGTTACA
    CAGTCTCAGG TAAAACCATT AGTGGTCCCA GCCATTAATA AGGACAAGCC GGAGCTTCCA
    TTTCTAAAGC CTCACAGAAG AACTTCCAGT CAGTATGTGG CCTCTGCCAT TGCAAAACAC
    ATCAATCTGC CTACCTTTAA GACTGATTCT ACAGATTTGC ATGAGAAAGA TGAAAATAAG
    CACGGGGCAG GAGAGGTTAA AACTGAAGCT GAAGTTTCCC CCAAAAGGTG TATTATTGTG
    GCCAAGTACA GCCCTGTGGA AACAGAATCA GCAGAAACAA GAGAAAAAAT TTCAAGTATT
    TTTACTTGTA GTCAAAAAGC ATCCCACAGA TTCACACCTG GTGATAATTC TGGCATGGAA
    AACAAAGTAG TGAACATCAG GGCACCGAGT CAAGCAACTC CTGCTAGTTT CTATAAAAAG
    AATGCCAGTG TCCCACTTAC TAAATCCTCT TCAAAGGATA ACAACAAAGC AGAAGATGAT
    CACAGTTTAA ACAGCAAACA AGGTACAGCA GAGAAAAAGA TGCATCTTTT GTTTGACCAG
    AAATACGAGA CTTTGCCCCG TAGTAATTCA GCCTCCTCTC TCAAGTCACC TGATCTCCAA
    GGAGCCTCAT CTTCTTTCAG TTCATCTTTG CGCATCACTA AATGGCCTCC TGCTGACAGC
    GTGCAAGTTA GTTCCCTGAA AGCATCGCCC GAGTTCAGTG GTGTGACAGC AAAGGCAGAA
    TCAGAACAGA ATGACAAATC TGATGATTCG GCTGCTAATG CTATTGGCGA ATTGGATGTT
    AATGTGCAGA CTAATATCTT CGGACCAAAG AAGAAGTTCA AACCTGTTGT CCAAAAACCA
    ATTCCGAAAG ATACATCACT GCACAGTGCC CTCATGGAAG CTATTCAAAC AGGAGGGGGA
    AAAGAGAAAC TCAGAAAGGT TTCAGACAGC GCGCTAAATG GCAATCACAA GAAACCATCA
    TACAGTGAGC CAGAGAATGA GCGGTCGGCC CTACTAGCGG CAATTAGAGG CCACAGTGGC
    ACTTGCAAGC TGAAAAAAAT CTCCTCATTG GCCTCCGAAG AGCTCCAGAG TTTTAGGAAT
    GCAGAACTGT CTTCACAGAA AGCAGAAGAC CCTCAGGAAG AGCAGCTCTG CATTCCACCT
    GCCCCTGCCC AGCCACCACC ACCGCCTCCC CCTCAGCTGT CAACAGCAAC TTTCAAATTC
    TCTGCCACAA CTACGGGTGA CCCTGGAGAG GCCAGGCGAG CCCTGATGGA GGCCATTCGC
    TCTGGTGCTG GAGCAGCAAA GTTAAAAAAG GTCCCTCTGC TCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137799.1
    CDS93..4280
    Translation

    Target ORF information:

    RefSeq Version XM_015282313.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282313.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAAGCTC GTGCTCCTCC TCCACCAAAC CAACCTTCTG CAACCAGTAG AATTCACAGT 
    GAGCACAAGT CACCTGCAGA GACAGCCGTA ATTTCTGATC AGAGCCTTGT GAGCATGAAG
    GAGAATATGA TAAATAGAAT GGTGGACTTC ACAGTCATTT TACCCAGCGG GGTGGAACAG
    AAGAGCACAG TACAAGGAAG TAAAGCTGTG ATGGATCTTT TGGTTGATCT ATGCAGCCGG
    TATCACTTGA ATCCATCCCA GTACAGTCTT GAATTGAAGT CTTTGGAAAC TCAACAACCT
    TTGAGTTACA AGCCAAACAC TTTGATAGGA GCACTGGACG TACAGACAGT ACTCCTGAAA
    GAGAAGGTTC CTGAAGAGAA GATCAAGAGA CCTCTGCCGA GGGCTCCTGA GAAGTCTGTG
    AGGCTGGTGG TAAATTACCT GAAGACACAG AAGGCAGTGG TTCGAGTCAG CCCGGACGTG
    CCCCTTCACA ACATCATCCC AGCTATTTGT GAAAAGTGTG AGGTCAGTCA GGAGCACATT
    GTTCTTCTGC GAGACAGCAT CACTGGCGAG GAGCTGGAGC TTACCAAATC CTTGGAGGAG
    CTGGGAATAA GCGAGCTGTA CGCCTGGGAC CGAAAAAGAG TTCCTCCATC AAAAACTCAG
    TCTGACCCTT CTCTGAACTA CAGAGAACCA AGTCGAAGTG TTTCACTAAG CAACGATGCA
    ATAGAAAAAG AAAAGAAAGG ATTTCTGGGG TTTTTCAAAG CTAATAGAAG CAACAGCAAG
    ACAGAAGAAC ACTTGAGGAT GAACAGAGAT TATGGTGAGG AGGATGTTTT TAGTTCTACA
    TCTACAAGTG AAGGAAGCTT GGATGGTTTT TCCACAGCAC CAAACTCCCC TTCGGTGAAT
    TCCCGTTCCA GTACCTTGGG TCCATCGCTG TCCCTTGGTA ATATCTCAGG AATGACAGCA
    AACCCAGAAG TGAAAAAGCG CAGAGCACCT CCTCCACCAG TTTTGACACC TCCGTTGCAG
    AATATGGAGA TGAGCTCCGC TCAGGAGAAG ACAGCAGTAC AGATTTCACA AGGAGCATCT
    CTTAACGATC TACAGAAAAA GAAGAGGCGT GCACCTCTTC CACCAACTCA GTCTGCTCCA
    CCTACTCCAG CCATGCCAAA TAGGAGGGAA GAGATGGAAG ACAAGAGGAA GAGCACAATG
    GGTGATGGAC GGCAGGTGCC ACAAAAGCCT CCTAGGGGCC CCACTCGTGA CCCACCCCAG
    TTAGTGATCC CCCCACCCCC ACCTTACCCT CCTCCTGAAA GAGACAATAT GGATCCAGCA
    GTCTCTTACA GAGAAGCAGA TGTTACAGCA CCAACAAAAC TGGTACCTAA ACAGAGCCTG
    CTGCCTGCAC ATGATAATTT TTATGTAGTG GATGATACAG TTATGGAGCT ATCAGAGGTA
    GAAGAAACAG CGTCAGAAAG CAGCTGCTTT GCATCTGAGG ACACTACCGA AGACTCAGGT
    GTAGTGAGCT CCCCATCTGA CATTGTGTCA CTGGATTCAC AAAATGACAG TATGAAGTTG
    AGAGACATCA AGCTGGTGAA TGGCTATGTG GACTCAGACA CGATCTATGG TGCAGAGACT
    TGCTCTGTGA AGAACGCGTA CTGCGATGAT ATGGGATATG CCGGTACTCC ACAGAGCACT
    AAAAATGAAG AAATGGCTGC GGCTAAGCAT GAGAATGAGG ACATATTCAT TGATGCACAA
    CTCCAGCAGA CTTTGGCTGA CCTGGATGAA GATTTAGAAG CTATGGATGT TTTAAGTAAC
    TCTGACTCTG AGCACATGAG TGAAGTGTTG AGCCCACATT CAGGAAAAGT TGAGGTCCCC
    CAAGATGTTG CTAGTTCTGT TCCAGTAACA GTCATAGATG ATGCTCCAGA AGTTACCATC
    TATAACTCAG CTGGAAACCA AGAGACAAGG GGTTCACAGA ATCTATCAGC TGACTCTGTT
    AATATGGGAA ACTTCACAAA TAGAAACAAC AATGCAGGTT CCTTTGATAA GGGACACACT
    GATGGTGGAG CTGTATGTAT ACCCAAGGAC CTAATACATC AGCAGCCATC TGATAATGAG
    TTCAGTCACA TACCCGTGGA ACAGAGGAGA GATATGTTTG AATCTCTCAC GTCCTCCTTT
    GAAAAAAGAA GTGAAAAGAA TGTAAGAGTA GAAGCTCCGC CCAGACACGT TATGAAGTCT
    GAGGAGTGTA AATCGAAGCT GACGTCATCT CACTCCAAGA CTGCGACTGA AATCAATACA
    GCAAAAGAGA AAGTAAATCC ATTTAATGAA TGTAGTAAGA GGGAGAGACC TCTGAAAAAA
    AGTAAGTCAG AACTAGAAAT CAAAAGGCCA CCAGCAAAAA AGATTGAAGT AGAAGAAGTT
    CCATCAACAC CTCCGTGGTG TCATCGTGCC CAGAAATTGG AAACAAGTTA TGAACCCAAA
    ATGGGTTTGA CGACCTTCAA AGTTGTCCCT CCCAAACCTG AAGTAAAAAA TTTTGATAGA
    GGGCTTTCAG TCTCCACTGG TGCTATTAAG ATTGATGAAC TAGGCAACCT TATCAACCCA
    AAGCCTGTTG TCAGTAAGAA CATACCAGGT ACCTCTGCTG GTGAAAGTGA GGAAATTCCT
    CTTGGGAAAG TGAAGGCATT CTGGAGGTCA AGTTCTATGG AAAAACAAAG TGATGACTCT
    GCTGAGCATC TTGCTAAAAA GCCAGCAGTT ACCACAAACT CTAAGTCCTT TGTTACAAAG
    CAAGAATGCA AACCTGTCTG TCTTGCAGCT CCAAAACCTG TACCACCACA AACTGTTATT
    TCCCAGGTAG GTGAAAAGCA GTCTGAGAAT GAGAAGACCA AACCACCTCT TCCAGTTACA
    CAGTCTCAGG TAAAACCATT AGTGGTCCCA GCCATTAATA AGGACAAGCC GGAGCTTCCA
    TTTCTAAAGC CTCACAGAAG AACTTCCAGT CAGTATGTGG CCTCTGCCAT TGCAAAACAC
    ATCAATCTGC CTACCTTTAA GACTGATTCT ACAGATTTGC ATGAGAAAGA TGAAAATAAG
    CACGGGGCAG GAGAGGTTAA AACTGAAGCT GAAGTTTCCC CCAAAAGGTG TATTATTGTG
    GCCAAGTACA GCCCTGTGGA AACAGAATCA GCAGAAACAA GAGAAAAAAT TTCAAGTATT
    TTTACTTGTA GTCAAAAAGC ATCCCACAGA TTCACACCTG GTGATAATTC TGGCATGGAA
    AACAAAGTAG TGAACATCAG GGCACCGAGT CAAGCAACTC CTGCTAGTTT CTATAAAAAG
    AATGCCAGTG TCCCACTTAC TAAATCCTCT TCAAAGGATA ACAACAAAGC AGAAGATGAT
    CACAGTTTAA ACAGCAAACA AGGTACAGCA GAGAAAAAGA TGCATCTTTT GTTTGACCAG
    AAATACGAGA CTTTGCCCCG TAGTAATTCA GCCTCCTCTC TCAAGTCACC TGATCTCCAA
    GGAGCCTCAT CTTCTTTCAG TTCATCTTTG CGCATCACTA AATGGCCTCC TGCTGACAGC
    GTGCAAGTTA GTTCCCTGAA AGCATCGCCC GAGTTCAGTG GTGTGACAGC AAAGGCAGAA
    TCAGAACAGA ATGACAAATC TGATGATTCG GCTGCTAATG CTATTGGCGA ATTGGATGTT
    AATGTGCAGA CTAATATCTT CGGACCAAAG AAGAAGTTCA AACCTGTTGT CCAAAAACCA
    ATTCCGAAAG ATACATCACT GCACAGTGCC CTCATGGAAG CTATTCAAAC AGGAGGGGGA
    AAAGAGAAAC TCAGAAAGGT TTCAGACAGC GCGCTAAATG GCAATCACAA GAAACCATCA
    TACAGTGAGC CAGAGAATGA GCGGTCGGCC CTACTAGCGG CAATTAGAGG CCACAGTGGC
    ACTTGCAAGC TGAAAAAAAT CTCCTCATTG GCCTCCGAAG AGCTCCAGAG TTTTAGGAAT
    GCAGAACTGT CTTCACAGAA AGCAGAAGAC CCTCAGGAAG AGCAGCTCTG CATTCCACCT
    GCCCCTGCCC AGCCACCACC ACCGCCTCCC CCTCAGCTGT CAACAGCAAC TTTCAAATTC
    TCTGCCACAA CTACGGGTGA CCCTGGAGAG GCCAGGCGAG CCCTGATGGA GGCCATTCGC
    TCTGGTGCTG GAGCAGCAAA GTTAAAAAAG GTCCCTCTGC TCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32269
    Clone ID Related Accession (Same CDS sequence) XM_015282315.1 , XM_015282315.2
    Accession Version XM_015282315.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4155bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product protein cordon-bleu isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455789.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGACTC TCGTCCGCCC CACGGCCGGC AAGCCGCCCA GCGGGAGAAG AATGAAAGCT 
    CGTGCTCCTC CTCCACCAAA CCAACCTTCT GCAACCAGTA GAATTCACAG TGAGCACAAG
    TCACCTGCAG AGACAGCCGT AATTTCTGAT CAGAGCCTTG TGAGCATGAA GGAGAATATG
    ATAAATAGAA TGGTGGACTT CACAGTCATT TTACCCAGCG GGGTGGAACA GAAGAGCACA
    GTACAAGGAA GTAAAGCTGT GATGGATCTT TTGGTTGATC TATGCAGCCG GTATCACTTG
    AATCCATCCC AGTACAGTCT TGAATTGAAG TCTTTGGAAA CTCAACAACC TTTGAGTTAC
    AAGCCAAACA CTTTGATAGG AGCACTGGAC GTACAGACAG TACTCCTGAA AGAGAAGGTT
    CCTGAAGAGA AGATCAAGAG ACCTCTGCCG AGGGCTCCTG AGAAGTCTGT GAGGCTGGTG
    GTAAATTACC TGAAGACACA GAAGGCAGTG GTTCGAGTCA GCCCGGACGT GCCCCTTCAC
    AACATCATCC CAGCTATTTG TGAAAAGTGT GAGGTCAGTC AGGAGCACAT TGTTCTTCTG
    CGAGACAGCA TCACTGGCGA GGAGCTGGAG CTTACCAAAT CCTTGGAGGA GCTGGGAATA
    AGCGAGCTGT ACGCCTGGGA CCGAAAAAGA GTTCCTCCAT CAAAAACTCA GTCTGACCCT
    TCTCTGAACT ACAGAGAACC AAGTCGAAGT GTTTCACTAA GCAACGATGC AATAGAAAAA
    GAAAAGAAAG GATTTCTGGG GTTTTTCAAA GCTAATAGAA GCAACAGCAA GGGTTTTTCC
    ACAGCACCAA ACTCCCCTTC GGTGAATTCC CGTTCCAGTA CCTTGGGTCC ATCGCTGTCC
    CTTGGTAATA TCTCAGGAAT GACAGCAAAC CCAGAAGTGA AAAAGCGCAG AGCACCTCCT
    CCACCAGTTT TGACACCTCC GTTGCAGAAT ATGGAGATGA GCTCCGCTCA GGAGAAGACA
    GCAGTACAGA TTTCACAAGG AGCATCTCTT AACGATCTAC AGAAAAAGAA GAGGCGTGCA
    CCTCTTCCAC CAACTCAGTC TGCTCCACCT ACTCCAGCCA TGCCAAATAG GAGGGAAGAG
    ATGGAAGACA AGAGGAAGAG CACAATGGGT GATGGACGGC AGGTGCCACA AAAGCCTCCT
    AGGGGCCCCA CTCGTGACCC ACCCCAGTTA GTGATCCCCC CACCCCCACC TTACCCTCCT
    CCTGAAAGAG ACAATATGGA TCCAGCAGTC TCTTACAGAG AAGCAGATGT TACAGCACCA
    ACAAAACTGG TACCTAAACA GAGCCTGCTG CCTGCACATG ATAATTTTTA TGTAGTGGAT
    GATACAGTTA TGGAGCTATC AGAGGTAGAA GAAACAGCGT CAGAAAGCAG CTGCTTTGCA
    TCTGAGGACA CTACCGAAGA CTCAGGTGTA GTGAGCTCCC CATCTGACAT TGTGTCACTG
    GATTCACAAA ATGACAGTAT GAAGTTGAGA GACATCAAGC TGGTGAATGG CTATGTGGAC
    TCAGACACGA TCTATGGTGC AGAGACTTGC TCTGTGAAGA ACGCGTACTG CGATGATATG
    GGATATGCCG GTACTCCACA GAGCACTAAA AATGAAGAAA TGGCTGCGGC TAAGCATGAG
    AATGAGGACA TATTCATTGA TGCACAACTC CAGCAGACTT TGGCTGACCT GGATGAAGAT
    TTAGAAGCTA TGGATGTTTT AAGTAACTCT GACTCTGAGC ACATGAGTGA AGTGTTGAGC
    CCACATTCAG GAAAAGTTGA GGTCCCCCAA GATGTTGCTA GTTCTGTTCC AGTAACAGTC
    ATAGATGATG CTCCAGAAGT TACCATCTAT AACTCAGCTG GAAACCAAGA GACAAGGGGT
    TCACAGAATC TATCAGCTGA CTCTGTTAAT ATGGGAAACT TCACAAATAG AAACAACAAT
    GCAGGTTCCT TTGATAAGGG ACACACTGAT GGTGGAGCTG TATGTATACC CAAGGACCTA
    ATACATCAGC AGCCATCTGA TAATGAGTTC AGTCACATAC CCGTGGAACA GAGGAGAGAT
    ATGTTTGAAT CTCTCACGTC CTCCTTTGAA AAAAGAAGTG AAAAGAATGT AAGAGTAGAA
    GCTCCGCCCA GACACGTTAT GAAGTCTGAG GAGTGTAAAT CGAAGCTGAC GTCATCTCAC
    TCCAAGACTG CGACTGAAAT CAATACAGCA AAAGAGAAAG TAAATCCATT TAATGAATGT
    AGTAAGAGGG AGAGACCTCT GAAAAAAAGT AAGTCAGAAC TAGAAATCAA AAGGCCACCA
    GCAAAAAAGA TTGAAGTAGA AGAAGTTCCA TCAACACCTC CGTGGTGTCA TCGTGCCCAG
    AAATTGGAAA CAAGTTATGA ACCCAAAATG GGTTTGACGA CCTTCAAAGT TGTCCCTCCC
    AAACCTGAAG TAAAAAATTT TGATAGAGGG CTTTCAGTCT CCACTGGTGC TATTAAGATT
    GATGAACTAG GCAACCTTAT CAACCCAAAG CCTGTTGTCA GTAAGAACAT ACCAGGTACC
    TCTGCTGGTG AAAGTGAGGA AATTCCTCTT GGGAAAGTGA AGGCATTCTG GAGGTCAAGT
    TCTATGGAAA AACAAAGTGA TGACTCTGCT GAGCATCTTG CTAAAAAGCC AGCAGTTACC
    ACAAACTCTA AGTCCTTTGT TACAAAGCAA GAATGCAAAC CTGTCTGTCT TGCAGCTCCA
    AAACCTGTAC CACCACAAAC TGTTATTTCC CAGGTAGGTG AAAAGCAGTC TGAGAATGAG
    AAGACCAAAC CACCTCTTCC AGTTACACAG TCTCAGGTAA AACCATTAGT GGTCCCAGCC
    ATTAATAAGG ACAAGCCGGA GCTTCCATTT CTAAAGCCTC ACAGAAGAAC TTCCAGTCAG
    TATGTGGCCT CTGCCATTGC AAAACACATC AATCTGCCTA CCTTTAAGAC TGATTCTACA
    GATTTGCATG AGAAAGATGA AAATAAGCAC GGGGCAGGAG AGGTTAAAAC TGAAGCTGAA
    GTTTCCCCCA AAAGGTGTAT TATTGTGGCC AAGTACAGCC CTGTGGAAAC AGAATCAGCA
    GAAACAAGAG AAAAAATTTC AAGTATTTTT ACTTGTAGTC AAAAAGCATC CCACAGATTC
    ACACCTGGTG ATAATTCTGG CATGGAAAAC AAAGTAGTGA ACATCAGGGC ACCGAGTCAA
    GCAACTCCTG CTAGTTTCTA TAAAAAGAAT GCCAGTGTCC CACTTACTAA ATCCTCTTCA
    AAGGATAACA ACAAAGCAGA AGATGATCAC AGTTTAAACA GCAAACAAGG TACAGCAGAG
    AAAAAGATGC ATCTTTTGTT TGACCAGAAA TACGAGACTT TGCCCCGTAG TAATTCAGCC
    TCCTCTCTCA AGTCACCTGA TCTCCAAGGA GCCTCATCTT CTTTCAGTTC ATCTTTGCGC
    ATCACTAAAT GGCCTCCTGC TGACAGCGTG CAAGTTAGTT CCCTGAAAGC ATCGCCCGAG
    TTCAGTGGTG TGACAGCAAA GGCAGAATCA GAACAGAATG ACAAATCTGA TGATTCGGCT
    GCTAATGCTA TTGGCGAATT GGATGTTAAT GTGCAGACTA ATATCTTCGG ACCAAAGAAG
    AAGTTCAAAC CTGTTGTCCA AAAACCAATT CCGAAAGATA CATCACTGCA CAGTGCCCTC
    ATGGAAGCTA TTCAAACAGG AGGGGGAAAA GAGAAACTCA GAAAGGTTTC AGACAGCGCG
    CTAAATGGCA ATCACAAGAA ACCATCATAC AGTGAGCCAG AGAATGAGCG GTCGGCCCTA
    CTAGCGGCAA TTAGAGGCCA CAGTGGCACT TGCAAGCTGA AAAAAATCTC CTCATTGGCC
    TCCGAAGAGC TCCAGAGTTT TAGGAATGCA GAACTGTCTT CACAGAAAGC AGAAGACCCT
    CAGGAAGAGC AGCTCTGCAT TCCACCTGCC CCTGCCCAGC CACCACCACC GCCTCCCCCT
    CAGCTGTCAA CAGCAACTTT CAAATTCTCT GCCACAACTA CGGGTGACCC TGGAGAGGCC
    AGGCGAGCCC TGATGGAGGC CATTCGCTCT GGTGCTGGAG CAGCAAAGTT AAAAAAGGTC
    CCTCTGCTCG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137801.1
    CDS194..4348
    Translation

    Target ORF information:

    RefSeq Version XM_015282315.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282315.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGACTC TCGTCCGCCC CACGGCCGGC AAGCCGCCCA GCGGGAGAAG AATGAAAGCT 
    CGTGCTCCTC CTCCACCAAA CCAACCTTCT GCAACCAGTA GAATTCACAG TGAGCACAAG
    TCACCTGCAG AGACAGCCGT AATTTCTGAT CAGAGCCTTG TGAGCATGAA GGAGAATATG
    ATAAATAGAA TGGTGGACTT CACAGTCATT TTACCCAGCG GGGTGGAACA GAAGAGCACA
    GTACAAGGAA GTAAAGCTGT GATGGATCTT TTGGTTGATC TATGCAGCCG GTATCACTTG
    AATCCATCCC AGTACAGTCT TGAATTGAAG TCTTTGGAAA CTCAACAACC TTTGAGTTAC
    AAGCCAAACA CTTTGATAGG AGCACTGGAC GTACAGACAG TACTCCTGAA AGAGAAGGTT
    CCTGAAGAGA AGATCAAGAG ACCTCTGCCG AGGGCTCCTG AGAAGTCTGT GAGGCTGGTG
    GTAAATTACC TGAAGACACA GAAGGCAGTG GTTCGAGTCA GCCCGGACGT GCCCCTTCAC
    AACATCATCC CAGCTATTTG TGAAAAGTGT GAGGTCAGTC AGGAGCACAT TGTTCTTCTG
    CGAGACAGCA TCACTGGCGA GGAGCTGGAG CTTACCAAAT CCTTGGAGGA GCTGGGAATA
    AGCGAGCTGT ACGCCTGGGA CCGAAAAAGA GTTCCTCCAT CAAAAACTCA GTCTGACCCT
    TCTCTGAACT ACAGAGAACC AAGTCGAAGT GTTTCACTAA GCAACGATGC AATAGAAAAA
    GAAAAGAAAG GATTTCTGGG GTTTTTCAAA GCTAATAGAA GCAACAGCAA GGGTTTTTCC
    ACAGCACCAA ACTCCCCTTC GGTGAATTCC CGTTCCAGTA CCTTGGGTCC ATCGCTGTCC
    CTTGGTAATA TCTCAGGAAT GACAGCAAAC CCAGAAGTGA AAAAGCGCAG AGCACCTCCT
    CCACCAGTTT TGACACCTCC GTTGCAGAAT ATGGAGATGA GCTCCGCTCA GGAGAAGACA
    GCAGTACAGA TTTCACAAGG AGCATCTCTT AACGATCTAC AGAAAAAGAA GAGGCGTGCA
    CCTCTTCCAC CAACTCAGTC TGCTCCACCT ACTCCAGCCA TGCCAAATAG GAGGGAAGAG
    ATGGAAGACA AGAGGAAGAG CACAATGGGT GATGGACGGC AGGTGCCACA AAAGCCTCCT
    AGGGGCCCCA CTCGTGACCC ACCCCAGTTA GTGATCCCCC CACCCCCACC TTACCCTCCT
    CCTGAAAGAG ACAATATGGA TCCAGCAGTC TCTTACAGAG AAGCAGATGT TACAGCACCA
    ACAAAACTGG TACCTAAACA GAGCCTGCTG CCTGCACATG ATAATTTTTA TGTAGTGGAT
    GATACAGTTA TGGAGCTATC AGAGGTAGAA GAAACAGCGT CAGAAAGCAG CTGCTTTGCA
    TCTGAGGACA CTACCGAAGA CTCAGGTGTA GTGAGCTCCC CATCTGACAT TGTGTCACTG
    GATTCACAAA ATGACAGTAT GAAGTTGAGA GACATCAAGC TGGTGAATGG CTATGTGGAC
    TCAGACACGA TCTATGGTGC AGAGACTTGC TCTGTGAAGA ACGCGTACTG CGATGATATG
    GGATATGCCG GTACTCCACA GAGCACTAAA AATGAAGAAA TGGCTGCGGC TAAGCATGAG
    AATGAGGACA TATTCATTGA TGCACAACTC CAGCAGACTT TGGCTGACCT GGATGAAGAT
    TTAGAAGCTA TGGATGTTTT AAGTAACTCT GACTCTGAGC ACATGAGTGA AGTGTTGAGC
    CCACATTCAG GAAAAGTTGA GGTCCCCCAA GATGTTGCTA GTTCTGTTCC AGTAACAGTC
    ATAGATGATG CTCCAGAAGT TACCATCTAT AACTCAGCTG GAAACCAAGA GACAAGGGGT
    TCACAGAATC TATCAGCTGA CTCTGTTAAT ATGGGAAACT TCACAAATAG AAACAACAAT
    GCAGGTTCCT TTGATAAGGG ACACACTGAT GGTGGAGCTG TATGTATACC CAAGGACCTA
    ATACATCAGC AGCCATCTGA TAATGAGTTC AGTCACATAC CCGTGGAACA GAGGAGAGAT
    ATGTTTGAAT CTCTCACGTC CTCCTTTGAA AAAAGAAGTG AAAAGAATGT AAGAGTAGAA
    GCTCCGCCCA GACACGTTAT GAAGTCTGAG GAGTGTAAAT CGAAGCTGAC GTCATCTCAC
    TCCAAGACTG CGACTGAAAT CAATACAGCA AAAGAGAAAG TAAATCCATT TAATGAATGT
    AGTAAGAGGG AGAGACCTCT GAAAAAAAGT AAGTCAGAAC TAGAAATCAA AAGGCCACCA
    GCAAAAAAGA TTGAAGTAGA AGAAGTTCCA TCAACACCTC CGTGGTGTCA TCGTGCCCAG
    AAATTGGAAA CAAGTTATGA ACCCAAAATG GGTTTGACGA CCTTCAAAGT TGTCCCTCCC
    AAACCTGAAG TAAAAAATTT TGATAGAGGG CTTTCAGTCT CCACTGGTGC TATTAAGATT
    GATGAACTAG GCAACCTTAT CAACCCAAAG CCTGTTGTCA GTAAGAACAT ACCAGGTACC
    TCTGCTGGTG AAAGTGAGGA AATTCCTCTT GGGAAAGTGA AGGCATTCTG GAGGTCAAGT
    TCTATGGAAA AACAAAGTGA TGACTCTGCT GAGCATCTTG CTAAAAAGCC AGCAGTTACC
    ACAAACTCTA AGTCCTTTGT TACAAAGCAA GAATGCAAAC CTGTCTGTCT TGCAGCTCCA
    AAACCTGTAC CACCACAAAC TGTTATTTCC CAGGTAGGTG AAAAGCAGTC TGAGAATGAG
    AAGACCAAAC CACCTCTTCC AGTTACACAG TCTCAGGTAA AACCATTAGT GGTCCCAGCC
    ATTAATAAGG ACAAGCCGGA GCTTCCATTT CTAAAGCCTC ACAGAAGAAC TTCCAGTCAG
    TATGTGGCCT CTGCCATTGC AAAACACATC AATCTGCCTA CCTTTAAGAC TGATTCTACA
    GATTTGCATG AGAAAGATGA AAATAAGCAC GGGGCAGGAG AGGTTAAAAC TGAAGCTGAA
    GTTTCCCCCA AAAGGTGTAT TATTGTGGCC AAGTACAGCC CTGTGGAAAC AGAATCAGCA
    GAAACAAGAG AAAAAATTTC AAGTATTTTT ACTTGTAGTC AAAAAGCATC CCACAGATTC
    ACACCTGGTG ATAATTCTGG CATGGAAAAC AAAGTAGTGA ACATCAGGGC ACCGAGTCAA
    GCAACTCCTG CTAGTTTCTA TAAAAAGAAT GCCAGTGTCC CACTTACTAA ATCCTCTTCA
    AAGGATAACA ACAAAGCAGA AGATGATCAC AGTTTAAACA GCAAACAAGG TACAGCAGAG
    AAAAAGATGC ATCTTTTGTT TGACCAGAAA TACGAGACTT TGCCCCGTAG TAATTCAGCC
    TCCTCTCTCA AGTCACCTGA TCTCCAAGGA GCCTCATCTT CTTTCAGTTC ATCTTTGCGC
    ATCACTAAAT GGCCTCCTGC TGACAGCGTG CAAGTTAGTT CCCTGAAAGC ATCGCCCGAG
    TTCAGTGGTG TGACAGCAAA GGCAGAATCA GAACAGAATG ACAAATCTGA TGATTCGGCT
    GCTAATGCTA TTGGCGAATT GGATGTTAAT GTGCAGACTA ATATCTTCGG ACCAAAGAAG
    AAGTTCAAAC CTGTTGTCCA AAAACCAATT CCGAAAGATA CATCACTGCA CAGTGCCCTC
    ATGGAAGCTA TTCAAACAGG AGGGGGAAAA GAGAAACTCA GAAAGGTTTC AGACAGCGCG
    CTAAATGGCA ATCACAAGAA ACCATCATAC AGTGAGCCAG AGAATGAGCG GTCGGCCCTA
    CTAGCGGCAA TTAGAGGCCA CAGTGGCACT TGCAAGCTGA AAAAAATCTC CTCATTGGCC
    TCCGAAGAGC TCCAGAGTTT TAGGAATGCA GAACTGTCTT CACAGAAAGC AGAAGACCCT
    CAGGAAGAGC AGCTCTGCAT TCCACCTGCC CCTGCCCAGC CACCACCACC GCCTCCCCCT
    CAGCTGTCAA CAGCAACTTT CAAATTCTCT GCCACAACTA CGGGTGACCC TGGAGAGGCC
    AGGCGAGCCC TGATGGAGGC CATTCGCTCT GGTGCTGGAG CAGCAAAGTT AAAAAAGGTC
    CCTCTGCTCG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32269
    Clone ID Related Accession (Same CDS sequence) XM_015282315.1 , XM_015282315.2
    Accession Version XM_015282315.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4155bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein cordon-bleu isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015282315.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGACTC TCGTCCGCCC CACGGCCGGC AAGCCGCCCA GCGGGAGAAG AATGAAAGCT 
    CGTGCTCCTC CTCCACCAAA CCAACCTTCT GCAACCAGTA GAATTCACAG TGAGCACAAG
    TCACCTGCAG AGACAGCCGT AATTTCTGAT CAGAGCCTTG TGAGCATGAA GGAGAATATG
    ATAAATAGAA TGGTGGACTT CACAGTCATT TTACCCAGCG GGGTGGAACA GAAGAGCACA
    GTACAAGGAA GTAAAGCTGT GATGGATCTT TTGGTTGATC TATGCAGCCG GTATCACTTG
    AATCCATCCC AGTACAGTCT TGAATTGAAG TCTTTGGAAA CTCAACAACC TTTGAGTTAC
    AAGCCAAACA CTTTGATAGG AGCACTGGAC GTACAGACAG TACTCCTGAA AGAGAAGGTT
    CCTGAAGAGA AGATCAAGAG ACCTCTGCCG AGGGCTCCTG AGAAGTCTGT GAGGCTGGTG
    GTAAATTACC TGAAGACACA GAAGGCAGTG GTTCGAGTCA GCCCGGACGT GCCCCTTCAC
    AACATCATCC CAGCTATTTG TGAAAAGTGT GAGGTCAGTC AGGAGCACAT TGTTCTTCTG
    CGAGACAGCA TCACTGGCGA GGAGCTGGAG CTTACCAAAT CCTTGGAGGA GCTGGGAATA
    AGCGAGCTGT ACGCCTGGGA CCGAAAAAGA GTTCCTCCAT CAAAAACTCA GTCTGACCCT
    TCTCTGAACT ACAGAGAACC AAGTCGAAGT GTTTCACTAA GCAACGATGC AATAGAAAAA
    GAAAAGAAAG GATTTCTGGG GTTTTTCAAA GCTAATAGAA GCAACAGCAA GGGTTTTTCC
    ACAGCACCAA ACTCCCCTTC GGTGAATTCC CGTTCCAGTA CCTTGGGTCC ATCGCTGTCC
    CTTGGTAATA TCTCAGGAAT GACAGCAAAC CCAGAAGTGA AAAAGCGCAG AGCACCTCCT
    CCACCAGTTT TGACACCTCC GTTGCAGAAT ATGGAGATGA GCTCCGCTCA GGAGAAGACA
    GCAGTACAGA TTTCACAAGG AGCATCTCTT AACGATCTAC AGAAAAAGAA GAGGCGTGCA
    CCTCTTCCAC CAACTCAGTC TGCTCCACCT ACTCCAGCCA TGCCAAATAG GAGGGAAGAG
    ATGGAAGACA AGAGGAAGAG CACAATGGGT GATGGACGGC AGGTGCCACA AAAGCCTCCT
    AGGGGCCCCA CTCGTGACCC ACCCCAGTTA GTGATCCCCC CACCCCCACC TTACCCTCCT
    CCTGAAAGAG ACAATATGGA TCCAGCAGTC TCTTACAGAG AAGCAGATGT TACAGCACCA
    ACAAAACTGG TACCTAAACA GAGCCTGCTG CCTGCACATG ATAATTTTTA TGTAGTGGAT
    GATACAGTTA TGGAGCTATC AGAGGTAGAA GAAACAGCGT CAGAAAGCAG CTGCTTTGCA
    TCTGAGGACA CTACCGAAGA CTCAGGTGTA GTGAGCTCCC CATCTGACAT TGTGTCACTG
    GATTCACAAA ATGACAGTAT GAAGTTGAGA GACATCAAGC TGGTGAATGG CTATGTGGAC
    TCAGACACGA TCTATGGTGC AGAGACTTGC TCTGTGAAGA ACGCGTACTG CGATGATATG
    GGATATGCCG GTACTCCACA GAGCACTAAA AATGAAGAAA TGGCTGCGGC TAAGCATGAG
    AATGAGGACA TATTCATTGA TGCACAACTC CAGCAGACTT TGGCTGACCT GGATGAAGAT
    TTAGAAGCTA TGGATGTTTT AAGTAACTCT GACTCTGAGC ACATGAGTGA AGTGTTGAGC
    CCACATTCAG GAAAAGTTGA GGTCCCCCAA GATGTTGCTA GTTCTGTTCC AGTAACAGTC
    ATAGATGATG CTCCAGAAGT TACCATCTAT AACTCAGCTG GAAACCAAGA GACAAGGGGT
    TCACAGAATC TATCAGCTGA CTCTGTTAAT ATGGGAAACT TCACAAATAG AAACAACAAT
    GCAGGTTCCT TTGATAAGGG ACACACTGAT GGTGGAGCTG TATGTATACC CAAGGACCTA
    ATACATCAGC AGCCATCTGA TAATGAGTTC AGTCACATAC CCGTGGAACA GAGGAGAGAT
    ATGTTTGAAT CTCTCACGTC CTCCTTTGAA AAAAGAAGTG AAAAGAATGT AAGAGTAGAA
    GCTCCGCCCA GACACGTTAT GAAGTCTGAG GAGTGTAAAT CGAAGCTGAC GTCATCTCAC
    TCCAAGACTG CGACTGAAAT CAATACAGCA AAAGAGAAAG TAAATCCATT TAATGAATGT
    AGTAAGAGGG AGAGACCTCT GAAAAAAAGT AAGTCAGAAC TAGAAATCAA AAGGCCACCA
    GCAAAAAAGA TTGAAGTAGA AGAAGTTCCA TCAACACCTC CGTGGTGTCA TCGTGCCCAG
    AAATTGGAAA CAAGTTATGA ACCCAAAATG GGTTTGACGA CCTTCAAAGT TGTCCCTCCC
    AAACCTGAAG TAAAAAATTT TGATAGAGGG CTTTCAGTCT CCACTGGTGC TATTAAGATT
    GATGAACTAG GCAACCTTAT CAACCCAAAG CCTGTTGTCA GTAAGAACAT ACCAGGTACC
    TCTGCTGGTG AAAGTGAGGA AATTCCTCTT GGGAAAGTGA AGGCATTCTG GAGGTCAAGT
    TCTATGGAAA AACAAAGTGA TGACTCTGCT GAGCATCTTG CTAAAAAGCC AGCAGTTACC
    ACAAACTCTA AGTCCTTTGT TACAAAGCAA GAATGCAAAC CTGTCTGTCT TGCAGCTCCA
    AAACCTGTAC CACCACAAAC TGTTATTTCC CAGGTAGGTG AAAAGCAGTC TGAGAATGAG
    AAGACCAAAC CACCTCTTCC AGTTACACAG TCTCAGGTAA AACCATTAGT GGTCCCAGCC
    ATTAATAAGG ACAAGCCGGA GCTTCCATTT CTAAAGCCTC ACAGAAGAAC TTCCAGTCAG
    TATGTGGCCT CTGCCATTGC AAAACACATC AATCTGCCTA CCTTTAAGAC TGATTCTACA
    GATTTGCATG AGAAAGATGA AAATAAGCAC GGGGCAGGAG AGGTTAAAAC TGAAGCTGAA
    GTTTCCCCCA AAAGGTGTAT TATTGTGGCC AAGTACAGCC CTGTGGAAAC AGAATCAGCA
    GAAACAAGAG AAAAAATTTC AAGTATTTTT ACTTGTAGTC AAAAAGCATC CCACAGATTC
    ACACCTGGTG ATAATTCTGG CATGGAAAAC AAAGTAGTGA ACATCAGGGC ACCGAGTCAA
    GCAACTCCTG CTAGTTTCTA TAAAAAGAAT GCCAGTGTCC CACTTACTAA ATCCTCTTCA
    AAGGATAACA ACAAAGCAGA AGATGATCAC AGTTTAAACA GCAAACAAGG TACAGCAGAG
    AAAAAGATGC ATCTTTTGTT TGACCAGAAA TACGAGACTT TGCCCCGTAG TAATTCAGCC
    TCCTCTCTCA AGTCACCTGA TCTCCAAGGA GCCTCATCTT CTTTCAGTTC ATCTTTGCGC
    ATCACTAAAT GGCCTCCTGC TGACAGCGTG CAAGTTAGTT CCCTGAAAGC ATCGCCCGAG
    TTCAGTGGTG TGACAGCAAA GGCAGAATCA GAACAGAATG ACAAATCTGA TGATTCGGCT
    GCTAATGCTA TTGGCGAATT GGATGTTAAT GTGCAGACTA ATATCTTCGG ACCAAAGAAG
    AAGTTCAAAC CTGTTGTCCA AAAACCAATT CCGAAAGATA CATCACTGCA CAGTGCCCTC
    ATGGAAGCTA TTCAAACAGG AGGGGGAAAA GAGAAACTCA GAAAGGTTTC AGACAGCGCG
    CTAAATGGCA ATCACAAGAA ACCATCATAC AGTGAGCCAG AGAATGAGCG GTCGGCCCTA
    CTAGCGGCAA TTAGAGGCCA CAGTGGCACT TGCAAGCTGA AAAAAATCTC CTCATTGGCC
    TCCGAAGAGC TCCAGAGTTT TAGGAATGCA GAACTGTCTT CACAGAAAGC AGAAGACCCT
    CAGGAAGAGC AGCTCTGCAT TCCACCTGCC CCTGCCCAGC CACCACCACC GCCTCCCCCT
    CAGCTGTCAA CAGCAACTTT CAAATTCTCT GCCACAACTA CGGGTGACCC TGGAGAGGCC
    AGGCGAGCCC TGATGGAGGC CATTCGCTCT GGTGCTGGAG CAGCAAAGTT AAAAAAGGTC
    CCTCTGCTCG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137801.1
    CDS367..4521
    Translation

    Target ORF information:

    RefSeq Version XM_015282315.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282315.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGACTC TCGTCCGCCC CACGGCCGGC AAGCCGCCCA GCGGGAGAAG AATGAAAGCT 
    CGTGCTCCTC CTCCACCAAA CCAACCTTCT GCAACCAGTA GAATTCACAG TGAGCACAAG
    TCACCTGCAG AGACAGCCGT AATTTCTGAT CAGAGCCTTG TGAGCATGAA GGAGAATATG
    ATAAATAGAA TGGTGGACTT CACAGTCATT TTACCCAGCG GGGTGGAACA GAAGAGCACA
    GTACAAGGAA GTAAAGCTGT GATGGATCTT TTGGTTGATC TATGCAGCCG GTATCACTTG
    AATCCATCCC AGTACAGTCT TGAATTGAAG TCTTTGGAAA CTCAACAACC TTTGAGTTAC
    AAGCCAAACA CTTTGATAGG AGCACTGGAC GTACAGACAG TACTCCTGAA AGAGAAGGTT
    CCTGAAGAGA AGATCAAGAG ACCTCTGCCG AGGGCTCCTG AGAAGTCTGT GAGGCTGGTG
    GTAAATTACC TGAAGACACA GAAGGCAGTG GTTCGAGTCA GCCCGGACGT GCCCCTTCAC
    AACATCATCC CAGCTATTTG TGAAAAGTGT GAGGTCAGTC AGGAGCACAT TGTTCTTCTG
    CGAGACAGCA TCACTGGCGA GGAGCTGGAG CTTACCAAAT CCTTGGAGGA GCTGGGAATA
    AGCGAGCTGT ACGCCTGGGA CCGAAAAAGA GTTCCTCCAT CAAAAACTCA GTCTGACCCT
    TCTCTGAACT ACAGAGAACC AAGTCGAAGT GTTTCACTAA GCAACGATGC AATAGAAAAA
    GAAAAGAAAG GATTTCTGGG GTTTTTCAAA GCTAATAGAA GCAACAGCAA GGGTTTTTCC
    ACAGCACCAA ACTCCCCTTC GGTGAATTCC CGTTCCAGTA CCTTGGGTCC ATCGCTGTCC
    CTTGGTAATA TCTCAGGAAT GACAGCAAAC CCAGAAGTGA AAAAGCGCAG AGCACCTCCT
    CCACCAGTTT TGACACCTCC GTTGCAGAAT ATGGAGATGA GCTCCGCTCA GGAGAAGACA
    GCAGTACAGA TTTCACAAGG AGCATCTCTT AACGATCTAC AGAAAAAGAA GAGGCGTGCA
    CCTCTTCCAC CAACTCAGTC TGCTCCACCT ACTCCAGCCA TGCCAAATAG GAGGGAAGAG
    ATGGAAGACA AGAGGAAGAG CACAATGGGT GATGGACGGC AGGTGCCACA AAAGCCTCCT
    AGGGGCCCCA CTCGTGACCC ACCCCAGTTA GTGATCCCCC CACCCCCACC TTACCCTCCT
    CCTGAAAGAG ACAATATGGA TCCAGCAGTC TCTTACAGAG AAGCAGATGT TACAGCACCA
    ACAAAACTGG TACCTAAACA GAGCCTGCTG CCTGCACATG ATAATTTTTA TGTAGTGGAT
    GATACAGTTA TGGAGCTATC AGAGGTAGAA GAAACAGCGT CAGAAAGCAG CTGCTTTGCA
    TCTGAGGACA CTACCGAAGA CTCAGGTGTA GTGAGCTCCC CATCTGACAT TGTGTCACTG
    GATTCACAAA ATGACAGTAT GAAGTTGAGA GACATCAAGC TGGTGAATGG CTATGTGGAC
    TCAGACACGA TCTATGGTGC AGAGACTTGC TCTGTGAAGA ACGCGTACTG CGATGATATG
    GGATATGCCG GTACTCCACA GAGCACTAAA AATGAAGAAA TGGCTGCGGC TAAGCATGAG
    AATGAGGACA TATTCATTGA TGCACAACTC CAGCAGACTT TGGCTGACCT GGATGAAGAT
    TTAGAAGCTA TGGATGTTTT AAGTAACTCT GACTCTGAGC ACATGAGTGA AGTGTTGAGC
    CCACATTCAG GAAAAGTTGA GGTCCCCCAA GATGTTGCTA GTTCTGTTCC AGTAACAGTC
    ATAGATGATG CTCCAGAAGT TACCATCTAT AACTCAGCTG GAAACCAAGA GACAAGGGGT
    TCACAGAATC TATCAGCTGA CTCTGTTAAT ATGGGAAACT TCACAAATAG AAACAACAAT
    GCAGGTTCCT TTGATAAGGG ACACACTGAT GGTGGAGCTG TATGTATACC CAAGGACCTA
    ATACATCAGC AGCCATCTGA TAATGAGTTC AGTCACATAC CCGTGGAACA GAGGAGAGAT
    ATGTTTGAAT CTCTCACGTC CTCCTTTGAA AAAAGAAGTG AAAAGAATGT AAGAGTAGAA
    GCTCCGCCCA GACACGTTAT GAAGTCTGAG GAGTGTAAAT CGAAGCTGAC GTCATCTCAC
    TCCAAGACTG CGACTGAAAT CAATACAGCA AAAGAGAAAG TAAATCCATT TAATGAATGT
    AGTAAGAGGG AGAGACCTCT GAAAAAAAGT AAGTCAGAAC TAGAAATCAA AAGGCCACCA
    GCAAAAAAGA TTGAAGTAGA AGAAGTTCCA TCAACACCTC CGTGGTGTCA TCGTGCCCAG
    AAATTGGAAA CAAGTTATGA ACCCAAAATG GGTTTGACGA CCTTCAAAGT TGTCCCTCCC
    AAACCTGAAG TAAAAAATTT TGATAGAGGG CTTTCAGTCT CCACTGGTGC TATTAAGATT
    GATGAACTAG GCAACCTTAT CAACCCAAAG CCTGTTGTCA GTAAGAACAT ACCAGGTACC
    TCTGCTGGTG AAAGTGAGGA AATTCCTCTT GGGAAAGTGA AGGCATTCTG GAGGTCAAGT
    TCTATGGAAA AACAAAGTGA TGACTCTGCT GAGCATCTTG CTAAAAAGCC AGCAGTTACC
    ACAAACTCTA AGTCCTTTGT TACAAAGCAA GAATGCAAAC CTGTCTGTCT TGCAGCTCCA
    AAACCTGTAC CACCACAAAC TGTTATTTCC CAGGTAGGTG AAAAGCAGTC TGAGAATGAG
    AAGACCAAAC CACCTCTTCC AGTTACACAG TCTCAGGTAA AACCATTAGT GGTCCCAGCC
    ATTAATAAGG ACAAGCCGGA GCTTCCATTT CTAAAGCCTC ACAGAAGAAC TTCCAGTCAG
    TATGTGGCCT CTGCCATTGC AAAACACATC AATCTGCCTA CCTTTAAGAC TGATTCTACA
    GATTTGCATG AGAAAGATGA AAATAAGCAC GGGGCAGGAG AGGTTAAAAC TGAAGCTGAA
    GTTTCCCCCA AAAGGTGTAT TATTGTGGCC AAGTACAGCC CTGTGGAAAC AGAATCAGCA
    GAAACAAGAG AAAAAATTTC AAGTATTTTT ACTTGTAGTC AAAAAGCATC CCACAGATTC
    ACACCTGGTG ATAATTCTGG CATGGAAAAC AAAGTAGTGA ACATCAGGGC ACCGAGTCAA
    GCAACTCCTG CTAGTTTCTA TAAAAAGAAT GCCAGTGTCC CACTTACTAA ATCCTCTTCA
    AAGGATAACA ACAAAGCAGA AGATGATCAC AGTTTAAACA GCAAACAAGG TACAGCAGAG
    AAAAAGATGC ATCTTTTGTT TGACCAGAAA TACGAGACTT TGCCCCGTAG TAATTCAGCC
    TCCTCTCTCA AGTCACCTGA TCTCCAAGGA GCCTCATCTT CTTTCAGTTC ATCTTTGCGC
    ATCACTAAAT GGCCTCCTGC TGACAGCGTG CAAGTTAGTT CCCTGAAAGC ATCGCCCGAG
    TTCAGTGGTG TGACAGCAAA GGCAGAATCA GAACAGAATG ACAAATCTGA TGATTCGGCT
    GCTAATGCTA TTGGCGAATT GGATGTTAAT GTGCAGACTA ATATCTTCGG ACCAAAGAAG
    AAGTTCAAAC CTGTTGTCCA AAAACCAATT CCGAAAGATA CATCACTGCA CAGTGCCCTC
    ATGGAAGCTA TTCAAACAGG AGGGGGAAAA GAGAAACTCA GAAAGGTTTC AGACAGCGCG
    CTAAATGGCA ATCACAAGAA ACCATCATAC AGTGAGCCAG AGAATGAGCG GTCGGCCCTA
    CTAGCGGCAA TTAGAGGCCA CAGTGGCACT TGCAAGCTGA AAAAAATCTC CTCATTGGCC
    TCCGAAGAGC TCCAGAGTTT TAGGAATGCA GAACTGTCTT CACAGAAAGC AGAAGACCCT
    CAGGAAGAGC AGCTCTGCAT TCCACCTGCC CCTGCCCAGC CACCACCACC GCCTCCCCCT
    CAGCTGTCAA CAGCAACTTT CAAATTCTCT GCCACAACTA CGGGTGACCC TGGAGAGGCC
    AGGCGAGCCC TGATGGAGGC CATTCGCTCT GGTGCTGGAG CAGCAAAGTT AAAAAAGGTC
    CCTCTGCTCG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32270
    Clone ID Related Accession (Same CDS sequence) XM_015282316.1 , XM_015282316.2
    Accession Version XM_015282316.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4110bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product protein cordon-bleu isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455789.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGACTC TCGTCCGCCC CACGGCCGGC AAGCCGCCCA GCGGGAGAAG AATGAAAGCT 
    CGTGCTCCTC CTCCACCAAA CCAACCTTCT GCAACCAGTA GAATTCACAG TGAGCACAAG
    TCACCTGCAG AGACAGCCGT AATTTCTGAT CAGAGCCTTG TGAGCATGAA GGAGAATATG
    ATAAATAGAA TGGTGGACTT CACAGTCATT TTACCCAGCG GGGTGGAACA GAAGAGCACA
    GTACAAGGAA GTAAAGCTGT GATGGATCTT TTGGTTGATC TATGCAGCCG GTATCACTTG
    AATCCATCCC AGTACAGTCT TGAATTGAAG TCTTTGGAAA CTCAACAACC TTTGAGTTAC
    AAGCCAAACA CTTTGATAGG AGCACTGGAC GTACAGACAG TACTCCTGAA AGAGAAGGTT
    CCTGAAGAGA AGATCAAGAG ACCTCTGCCG AGGGCTCCTG AGAAGTCTGT GAGGCTGGTG
    GTAAATTACC TGAAGACACA GAAGGCAGTG GTTCGAGTCA GCCCGGACGT GCCCCTTCAC
    AACATCATCC CAGCTATTTG TGAAAAGTGT GAGGTCAGTC AGGAGCACAT TGTTCTTCTG
    CGAGACAGCA TCACTGGCGA GGAGCTGGAG CTTACCAAAT CCTTGGAGGA GCTGGGAATA
    AGCGAGCTGT ACGCCTGGGA CCGAAAAAGA GAACCAAGTC GAAGTGTTTC ACTAAGCAAC
    GATGCAATAG AAAAAGAAAA GAAAGGATTT CTGGGGTTTT TCAAAGCTAA TAGAAGCAAC
    AGCAAGGGTT TTTCCACAGC ACCAAACTCC CCTTCGGTGA ATTCCCGTTC CAGTACCTTG
    GGTCCATCGC TGTCCCTTGG TAATATCTCA GGAATGACAG CAAACCCAGA AGTGAAAAAG
    CGCAGAGCAC CTCCTCCACC AGTTTTGACA CCTCCGTTGC AGAATATGGA GATGAGCTCC
    GCTCAGGAGA AGACAGCAGT ACAGATTTCA CAAGGAGCAT CTCTTAACGA TCTACAGAAA
    AAGAAGAGGC GTGCACCTCT TCCACCAACT CAGTCTGCTC CACCTACTCC AGCCATGCCA
    AATAGGAGGG AAGAGATGGA AGACAAGAGG AAGAGCACAA TGGGTGATGG ACGGCAGGTG
    CCACAAAAGC CTCCTAGGGG CCCCACTCGT GACCCACCCC AGTTAGTGAT CCCCCCACCC
    CCACCTTACC CTCCTCCTGA AAGAGACAAT ATGGATCCAG CAGTCTCTTA CAGAGAAGCA
    GATGTTACAG CACCAACAAA ACTGGTACCT AAACAGAGCC TGCTGCCTGC ACATGATAAT
    TTTTATGTAG TGGATGATAC AGTTATGGAG CTATCAGAGG TAGAAGAAAC AGCGTCAGAA
    AGCAGCTGCT TTGCATCTGA GGACACTACC GAAGACTCAG GTGTAGTGAG CTCCCCATCT
    GACATTGTGT CACTGGATTC ACAAAATGAC AGTATGAAGT TGAGAGACAT CAAGCTGGTG
    AATGGCTATG TGGACTCAGA CACGATCTAT GGTGCAGAGA CTTGCTCTGT GAAGAACGCG
    TACTGCGATG ATATGGGATA TGCCGGTACT CCACAGAGCA CTAAAAATGA AGAAATGGCT
    GCGGCTAAGC ATGAGAATGA GGACATATTC ATTGATGCAC AACTCCAGCA GACTTTGGCT
    GACCTGGATG AAGATTTAGA AGCTATGGAT GTTTTAAGTA ACTCTGACTC TGAGCACATG
    AGTGAAGTGT TGAGCCCACA TTCAGGAAAA GTTGAGGTCC CCCAAGATGT TGCTAGTTCT
    GTTCCAGTAA CAGTCATAGA TGATGCTCCA GAAGTTACCA TCTATAACTC AGCTGGAAAC
    CAAGAGACAA GGGGTTCACA GAATCTATCA GCTGACTCTG TTAATATGGG AAACTTCACA
    AATAGAAACA ACAATGCAGG TTCCTTTGAT AAGGGACACA CTGATGGTGG AGCTGTATGT
    ATACCCAAGG ACCTAATACA TCAGCAGCCA TCTGATAATG AGTTCAGTCA CATACCCGTG
    GAACAGAGGA GAGATATGTT TGAATCTCTC ACGTCCTCCT TTGAAAAAAG AAGTGAAAAG
    AATGTAAGAG TAGAAGCTCC GCCCAGACAC GTTATGAAGT CTGAGGAGTG TAAATCGAAG
    CTGACGTCAT CTCACTCCAA GACTGCGACT GAAATCAATA CAGCAAAAGA GAAAGTAAAT
    CCATTTAATG AATGTAGTAA GAGGGAGAGA CCTCTGAAAA AAAGTAAGTC AGAACTAGAA
    ATCAAAAGGC CACCAGCAAA AAAGATTGAA GTAGAAGAAG TTCCATCAAC ACCTCCGTGG
    TGTCATCGTG CCCAGAAATT GGAAACAAGT TATGAACCCA AAATGGGTTT GACGACCTTC
    AAAGTTGTCC CTCCCAAACC TGAAGTAAAA AATTTTGATA GAGGGCTTTC AGTCTCCACT
    GGTGCTATTA AGATTGATGA ACTAGGCAAC CTTATCAACC CAAAGCCTGT TGTCAGTAAG
    AACATACCAG GTACCTCTGC TGGTGAAAGT GAGGAAATTC CTCTTGGGAA AGTGAAGGCA
    TTCTGGAGGT CAAGTTCTAT GGAAAAACAA AGTGATGACT CTGCTGAGCA TCTTGCTAAA
    AAGCCAGCAG TTACCACAAA CTCTAAGTCC TTTGTTACAA AGCAAGAATG CAAACCTGTC
    TGTCTTGCAG CTCCAAAACC TGTACCACCA CAAACTGTTA TTTCCCAGGT AGGTGAAAAG
    CAGTCTGAGA ATGAGAAGAC CAAACCACCT CTTCCAGTTA CACAGTCTCA GGTAAAACCA
    TTAGTGGTCC CAGCCATTAA TAAGGACAAG CCGGAGCTTC CATTTCTAAA GCCTCACAGA
    AGAACTTCCA GTCAGTATGT GGCCTCTGCC ATTGCAAAAC ACATCAATCT GCCTACCTTT
    AAGACTGATT CTACAGATTT GCATGAGAAA GATGAAAATA AGCACGGGGC AGGAGAGGTT
    AAAACTGAAG CTGAAGTTTC CCCCAAAAGG TGTATTATTG TGGCCAAGTA CAGCCCTGTG
    GAAACAGAAT CAGCAGAAAC AAGAGAAAAA ATTTCAAGTA TTTTTACTTG TAGTCAAAAA
    GCATCCCACA GATTCACACC TGGTGATAAT TCTGGCATGG AAAACAAAGT AGTGAACATC
    AGGGCACCGA GTCAAGCAAC TCCTGCTAGT TTCTATAAAA AGAATGCCAG TGTCCCACTT
    ACTAAATCCT CTTCAAAGGA TAACAACAAA GCAGAAGATG ATCACAGTTT AAACAGCAAA
    CAAGGTACAG CAGAGAAAAA GATGCATCTT TTGTTTGACC AGAAATACGA GACTTTGCCC
    CGTAGTAATT CAGCCTCCTC TCTCAAGTCA CCTGATCTCC AAGGAGCCTC ATCTTCTTTC
    AGTTCATCTT TGCGCATCAC TAAATGGCCT CCTGCTGACA GCGTGCAAGT TAGTTCCCTG
    AAAGCATCGC CCGAGTTCAG TGGTGTGACA GCAAAGGCAG AATCAGAACA GAATGACAAA
    TCTGATGATT CGGCTGCTAA TGCTATTGGC GAATTGGATG TTAATGTGCA GACTAATATC
    TTCGGACCAA AGAAGAAGTT CAAACCTGTT GTCCAAAAAC CAATTCCGAA AGATACATCA
    CTGCACAGTG CCCTCATGGA AGCTATTCAA ACAGGAGGGG GAAAAGAGAA ACTCAGAAAG
    GTTTCAGACA GCGCGCTAAA TGGCAATCAC AAGAAACCAT CATACAGTGA GCCAGAGAAT
    GAGCGGTCGG CCCTACTAGC GGCAATTAGA GGCCACAGTG GCACTTGCAA GCTGAAAAAA
    ATCTCCTCAT TGGCCTCCGA AGAGCTCCAG AGTTTTAGGA ATGCAGAACT GTCTTCACAG
    AAAGCAGAAG ACCCTCAGGA AGAGCAGCTC TGCATTCCAC CTGCCCCTGC CCAGCCACCA
    CCACCGCCTC CCCCTCAGCT GTCAACAGCA ACTTTCAAAT TCTCTGCCAC AACTACGGGT
    GACCCTGGAG AGGCCAGGCG AGCCCTGATG GAGGCCATTC GCTCTGGTGC TGGAGCAGCA
    AAGTTAAAAA AGGTCCCTCT GCTCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137802.1
    CDS194..4303
    Translation

    Target ORF information:

    RefSeq Version XM_015282316.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282316.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGACTC TCGTCCGCCC CACGGCCGGC AAGCCGCCCA GCGGGAGAAG AATGAAAGCT 
    CGTGCTCCTC CTCCACCAAA CCAACCTTCT GCAACCAGTA GAATTCACAG TGAGCACAAG
    TCACCTGCAG AGACAGCCGT AATTTCTGAT CAGAGCCTTG TGAGCATGAA GGAGAATATG
    ATAAATAGAA TGGTGGACTT CACAGTCATT TTACCCAGCG GGGTGGAACA GAAGAGCACA
    GTACAAGGAA GTAAAGCTGT GATGGATCTT TTGGTTGATC TATGCAGCCG GTATCACTTG
    AATCCATCCC AGTACAGTCT TGAATTGAAG TCTTTGGAAA CTCAACAACC TTTGAGTTAC
    AAGCCAAACA CTTTGATAGG AGCACTGGAC GTACAGACAG TACTCCTGAA AGAGAAGGTT
    CCTGAAGAGA AGATCAAGAG ACCTCTGCCG AGGGCTCCTG AGAAGTCTGT GAGGCTGGTG
    GTAAATTACC TGAAGACACA GAAGGCAGTG GTTCGAGTCA GCCCGGACGT GCCCCTTCAC
    AACATCATCC CAGCTATTTG TGAAAAGTGT GAGGTCAGTC AGGAGCACAT TGTTCTTCTG
    CGAGACAGCA TCACTGGCGA GGAGCTGGAG CTTACCAAAT CCTTGGAGGA GCTGGGAATA
    AGCGAGCTGT ACGCCTGGGA CCGAAAAAGA GAACCAAGTC GAAGTGTTTC ACTAAGCAAC
    GATGCAATAG AAAAAGAAAA GAAAGGATTT CTGGGGTTTT TCAAAGCTAA TAGAAGCAAC
    AGCAAGGGTT TTTCCACAGC ACCAAACTCC CCTTCGGTGA ATTCCCGTTC CAGTACCTTG
    GGTCCATCGC TGTCCCTTGG TAATATCTCA GGAATGACAG CAAACCCAGA AGTGAAAAAG
    CGCAGAGCAC CTCCTCCACC AGTTTTGACA CCTCCGTTGC AGAATATGGA GATGAGCTCC
    GCTCAGGAGA AGACAGCAGT ACAGATTTCA CAAGGAGCAT CTCTTAACGA TCTACAGAAA
    AAGAAGAGGC GTGCACCTCT TCCACCAACT CAGTCTGCTC CACCTACTCC AGCCATGCCA
    AATAGGAGGG AAGAGATGGA AGACAAGAGG AAGAGCACAA TGGGTGATGG ACGGCAGGTG
    CCACAAAAGC CTCCTAGGGG CCCCACTCGT GACCCACCCC AGTTAGTGAT CCCCCCACCC
    CCACCTTACC CTCCTCCTGA AAGAGACAAT ATGGATCCAG CAGTCTCTTA CAGAGAAGCA
    GATGTTACAG CACCAACAAA ACTGGTACCT AAACAGAGCC TGCTGCCTGC ACATGATAAT
    TTTTATGTAG TGGATGATAC AGTTATGGAG CTATCAGAGG TAGAAGAAAC AGCGTCAGAA
    AGCAGCTGCT TTGCATCTGA GGACACTACC GAAGACTCAG GTGTAGTGAG CTCCCCATCT
    GACATTGTGT CACTGGATTC ACAAAATGAC AGTATGAAGT TGAGAGACAT CAAGCTGGTG
    AATGGCTATG TGGACTCAGA CACGATCTAT GGTGCAGAGA CTTGCTCTGT GAAGAACGCG
    TACTGCGATG ATATGGGATA TGCCGGTACT CCACAGAGCA CTAAAAATGA AGAAATGGCT
    GCGGCTAAGC ATGAGAATGA GGACATATTC ATTGATGCAC AACTCCAGCA GACTTTGGCT
    GACCTGGATG AAGATTTAGA AGCTATGGAT GTTTTAAGTA ACTCTGACTC TGAGCACATG
    AGTGAAGTGT TGAGCCCACA TTCAGGAAAA GTTGAGGTCC CCCAAGATGT TGCTAGTTCT
    GTTCCAGTAA CAGTCATAGA TGATGCTCCA GAAGTTACCA TCTATAACTC AGCTGGAAAC
    CAAGAGACAA GGGGTTCACA GAATCTATCA GCTGACTCTG TTAATATGGG AAACTTCACA
    AATAGAAACA ACAATGCAGG TTCCTTTGAT AAGGGACACA CTGATGGTGG AGCTGTATGT
    ATACCCAAGG ACCTAATACA TCAGCAGCCA TCTGATAATG AGTTCAGTCA CATACCCGTG
    GAACAGAGGA GAGATATGTT TGAATCTCTC ACGTCCTCCT TTGAAAAAAG AAGTGAAAAG
    AATGTAAGAG TAGAAGCTCC GCCCAGACAC GTTATGAAGT CTGAGGAGTG TAAATCGAAG
    CTGACGTCAT CTCACTCCAA GACTGCGACT GAAATCAATA CAGCAAAAGA GAAAGTAAAT
    CCATTTAATG AATGTAGTAA GAGGGAGAGA CCTCTGAAAA AAAGTAAGTC AGAACTAGAA
    ATCAAAAGGC CACCAGCAAA AAAGATTGAA GTAGAAGAAG TTCCATCAAC ACCTCCGTGG
    TGTCATCGTG CCCAGAAATT GGAAACAAGT TATGAACCCA AAATGGGTTT GACGACCTTC
    AAAGTTGTCC CTCCCAAACC TGAAGTAAAA AATTTTGATA GAGGGCTTTC AGTCTCCACT
    GGTGCTATTA AGATTGATGA ACTAGGCAAC CTTATCAACC CAAAGCCTGT TGTCAGTAAG
    AACATACCAG GTACCTCTGC TGGTGAAAGT GAGGAAATTC CTCTTGGGAA AGTGAAGGCA
    TTCTGGAGGT CAAGTTCTAT GGAAAAACAA AGTGATGACT CTGCTGAGCA TCTTGCTAAA
    AAGCCAGCAG TTACCACAAA CTCTAAGTCC TTTGTTACAA AGCAAGAATG CAAACCTGTC
    TGTCTTGCAG CTCCAAAACC TGTACCACCA CAAACTGTTA TTTCCCAGGT AGGTGAAAAG
    CAGTCTGAGA ATGAGAAGAC CAAACCACCT CTTCCAGTTA CACAGTCTCA GGTAAAACCA
    TTAGTGGTCC CAGCCATTAA TAAGGACAAG CCGGAGCTTC CATTTCTAAA GCCTCACAGA
    AGAACTTCCA GTCAGTATGT GGCCTCTGCC ATTGCAAAAC ACATCAATCT GCCTACCTTT
    AAGACTGATT CTACAGATTT GCATGAGAAA GATGAAAATA AGCACGGGGC AGGAGAGGTT
    AAAACTGAAG CTGAAGTTTC CCCCAAAAGG TGTATTATTG TGGCCAAGTA CAGCCCTGTG
    GAAACAGAAT CAGCAGAAAC AAGAGAAAAA ATTTCAAGTA TTTTTACTTG TAGTCAAAAA
    GCATCCCACA GATTCACACC TGGTGATAAT TCTGGCATGG AAAACAAAGT AGTGAACATC
    AGGGCACCGA GTCAAGCAAC TCCTGCTAGT TTCTATAAAA AGAATGCCAG TGTCCCACTT
    ACTAAATCCT CTTCAAAGGA TAACAACAAA GCAGAAGATG ATCACAGTTT AAACAGCAAA
    CAAGGTACAG CAGAGAAAAA GATGCATCTT TTGTTTGACC AGAAATACGA GACTTTGCCC
    CGTAGTAATT CAGCCTCCTC TCTCAAGTCA CCTGATCTCC AAGGAGCCTC ATCTTCTTTC
    AGTTCATCTT TGCGCATCAC TAAATGGCCT CCTGCTGACA GCGTGCAAGT TAGTTCCCTG
    AAAGCATCGC CCGAGTTCAG TGGTGTGACA GCAAAGGCAG AATCAGAACA GAATGACAAA
    TCTGATGATT CGGCTGCTAA TGCTATTGGC GAATTGGATG TTAATGTGCA GACTAATATC
    TTCGGACCAA AGAAGAAGTT CAAACCTGTT GTCCAAAAAC CAATTCCGAA AGATACATCA
    CTGCACAGTG CCCTCATGGA AGCTATTCAA ACAGGAGGGG GAAAAGAGAA ACTCAGAAAG
    GTTTCAGACA GCGCGCTAAA TGGCAATCAC AAGAAACCAT CATACAGTGA GCCAGAGAAT
    GAGCGGTCGG CCCTACTAGC GGCAATTAGA GGCCACAGTG GCACTTGCAA GCTGAAAAAA
    ATCTCCTCAT TGGCCTCCGA AGAGCTCCAG AGTTTTAGGA ATGCAGAACT GTCTTCACAG
    AAAGCAGAAG ACCCTCAGGA AGAGCAGCTC TGCATTCCAC CTGCCCCTGC CCAGCCACCA
    CCACCGCCTC CCCCTCAGCT GTCAACAGCA ACTTTCAAAT TCTCTGCCAC AACTACGGGT
    GACCCTGGAG AGGCCAGGCG AGCCCTGATG GAGGCCATTC GCTCTGGTGC TGGAGCAGCA
    AAGTTAAAAA AGGTCCCTCT GCTCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32270
    Clone ID Related Accession (Same CDS sequence) XM_015282316.1 , XM_015282316.2
    Accession Version XM_015282316.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4110bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein cordon-bleu isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015282316.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGACTC TCGTCCGCCC CACGGCCGGC AAGCCGCCCA GCGGGAGAAG AATGAAAGCT 
    CGTGCTCCTC CTCCACCAAA CCAACCTTCT GCAACCAGTA GAATTCACAG TGAGCACAAG
    TCACCTGCAG AGACAGCCGT AATTTCTGAT CAGAGCCTTG TGAGCATGAA GGAGAATATG
    ATAAATAGAA TGGTGGACTT CACAGTCATT TTACCCAGCG GGGTGGAACA GAAGAGCACA
    GTACAAGGAA GTAAAGCTGT GATGGATCTT TTGGTTGATC TATGCAGCCG GTATCACTTG
    AATCCATCCC AGTACAGTCT TGAATTGAAG TCTTTGGAAA CTCAACAACC TTTGAGTTAC
    AAGCCAAACA CTTTGATAGG AGCACTGGAC GTACAGACAG TACTCCTGAA AGAGAAGGTT
    CCTGAAGAGA AGATCAAGAG ACCTCTGCCG AGGGCTCCTG AGAAGTCTGT GAGGCTGGTG
    GTAAATTACC TGAAGACACA GAAGGCAGTG GTTCGAGTCA GCCCGGACGT GCCCCTTCAC
    AACATCATCC CAGCTATTTG TGAAAAGTGT GAGGTCAGTC AGGAGCACAT TGTTCTTCTG
    CGAGACAGCA TCACTGGCGA GGAGCTGGAG CTTACCAAAT CCTTGGAGGA GCTGGGAATA
    AGCGAGCTGT ACGCCTGGGA CCGAAAAAGA GAACCAAGTC GAAGTGTTTC ACTAAGCAAC
    GATGCAATAG AAAAAGAAAA GAAAGGATTT CTGGGGTTTT TCAAAGCTAA TAGAAGCAAC
    AGCAAGGGTT TTTCCACAGC ACCAAACTCC CCTTCGGTGA ATTCCCGTTC CAGTACCTTG
    GGTCCATCGC TGTCCCTTGG TAATATCTCA GGAATGACAG CAAACCCAGA AGTGAAAAAG
    CGCAGAGCAC CTCCTCCACC AGTTTTGACA CCTCCGTTGC AGAATATGGA GATGAGCTCC
    GCTCAGGAGA AGACAGCAGT ACAGATTTCA CAAGGAGCAT CTCTTAACGA TCTACAGAAA
    AAGAAGAGGC GTGCACCTCT TCCACCAACT CAGTCTGCTC CACCTACTCC AGCCATGCCA
    AATAGGAGGG AAGAGATGGA AGACAAGAGG AAGAGCACAA TGGGTGATGG ACGGCAGGTG
    CCACAAAAGC CTCCTAGGGG CCCCACTCGT GACCCACCCC AGTTAGTGAT CCCCCCACCC
    CCACCTTACC CTCCTCCTGA AAGAGACAAT ATGGATCCAG CAGTCTCTTA CAGAGAAGCA
    GATGTTACAG CACCAACAAA ACTGGTACCT AAACAGAGCC TGCTGCCTGC ACATGATAAT
    TTTTATGTAG TGGATGATAC AGTTATGGAG CTATCAGAGG TAGAAGAAAC AGCGTCAGAA
    AGCAGCTGCT TTGCATCTGA GGACACTACC GAAGACTCAG GTGTAGTGAG CTCCCCATCT
    GACATTGTGT CACTGGATTC ACAAAATGAC AGTATGAAGT TGAGAGACAT CAAGCTGGTG
    AATGGCTATG TGGACTCAGA CACGATCTAT GGTGCAGAGA CTTGCTCTGT GAAGAACGCG
    TACTGCGATG ATATGGGATA TGCCGGTACT CCACAGAGCA CTAAAAATGA AGAAATGGCT
    GCGGCTAAGC ATGAGAATGA GGACATATTC ATTGATGCAC AACTCCAGCA GACTTTGGCT
    GACCTGGATG AAGATTTAGA AGCTATGGAT GTTTTAAGTA ACTCTGACTC TGAGCACATG
    AGTGAAGTGT TGAGCCCACA TTCAGGAAAA GTTGAGGTCC CCCAAGATGT TGCTAGTTCT
    GTTCCAGTAA CAGTCATAGA TGATGCTCCA GAAGTTACCA TCTATAACTC AGCTGGAAAC
    CAAGAGACAA GGGGTTCACA GAATCTATCA GCTGACTCTG TTAATATGGG AAACTTCACA
    AATAGAAACA ACAATGCAGG TTCCTTTGAT AAGGGACACA CTGATGGTGG AGCTGTATGT
    ATACCCAAGG ACCTAATACA TCAGCAGCCA TCTGATAATG AGTTCAGTCA CATACCCGTG
    GAACAGAGGA GAGATATGTT TGAATCTCTC ACGTCCTCCT TTGAAAAAAG AAGTGAAAAG
    AATGTAAGAG TAGAAGCTCC GCCCAGACAC GTTATGAAGT CTGAGGAGTG TAAATCGAAG
    CTGACGTCAT CTCACTCCAA GACTGCGACT GAAATCAATA CAGCAAAAGA GAAAGTAAAT
    CCATTTAATG AATGTAGTAA GAGGGAGAGA CCTCTGAAAA AAAGTAAGTC AGAACTAGAA
    ATCAAAAGGC CACCAGCAAA AAAGATTGAA GTAGAAGAAG TTCCATCAAC ACCTCCGTGG
    TGTCATCGTG CCCAGAAATT GGAAACAAGT TATGAACCCA AAATGGGTTT GACGACCTTC
    AAAGTTGTCC CTCCCAAACC TGAAGTAAAA AATTTTGATA GAGGGCTTTC AGTCTCCACT
    GGTGCTATTA AGATTGATGA ACTAGGCAAC CTTATCAACC CAAAGCCTGT TGTCAGTAAG
    AACATACCAG GTACCTCTGC TGGTGAAAGT GAGGAAATTC CTCTTGGGAA AGTGAAGGCA
    TTCTGGAGGT CAAGTTCTAT GGAAAAACAA AGTGATGACT CTGCTGAGCA TCTTGCTAAA
    AAGCCAGCAG TTACCACAAA CTCTAAGTCC TTTGTTACAA AGCAAGAATG CAAACCTGTC
    TGTCTTGCAG CTCCAAAACC TGTACCACCA CAAACTGTTA TTTCCCAGGT AGGTGAAAAG
    CAGTCTGAGA ATGAGAAGAC CAAACCACCT CTTCCAGTTA CACAGTCTCA GGTAAAACCA
    TTAGTGGTCC CAGCCATTAA TAAGGACAAG CCGGAGCTTC CATTTCTAAA GCCTCACAGA
    AGAACTTCCA GTCAGTATGT GGCCTCTGCC ATTGCAAAAC ACATCAATCT GCCTACCTTT
    AAGACTGATT CTACAGATTT GCATGAGAAA GATGAAAATA AGCACGGGGC AGGAGAGGTT
    AAAACTGAAG CTGAAGTTTC CCCCAAAAGG TGTATTATTG TGGCCAAGTA CAGCCCTGTG
    GAAACAGAAT CAGCAGAAAC AAGAGAAAAA ATTTCAAGTA TTTTTACTTG TAGTCAAAAA
    GCATCCCACA GATTCACACC TGGTGATAAT TCTGGCATGG AAAACAAAGT AGTGAACATC
    AGGGCACCGA GTCAAGCAAC TCCTGCTAGT TTCTATAAAA AGAATGCCAG TGTCCCACTT
    ACTAAATCCT CTTCAAAGGA TAACAACAAA GCAGAAGATG ATCACAGTTT AAACAGCAAA
    CAAGGTACAG CAGAGAAAAA GATGCATCTT TTGTTTGACC AGAAATACGA GACTTTGCCC
    CGTAGTAATT CAGCCTCCTC TCTCAAGTCA CCTGATCTCC AAGGAGCCTC ATCTTCTTTC
    AGTTCATCTT TGCGCATCAC TAAATGGCCT CCTGCTGACA GCGTGCAAGT TAGTTCCCTG
    AAAGCATCGC CCGAGTTCAG TGGTGTGACA GCAAAGGCAG AATCAGAACA GAATGACAAA
    TCTGATGATT CGGCTGCTAA TGCTATTGGC GAATTGGATG TTAATGTGCA GACTAATATC
    TTCGGACCAA AGAAGAAGTT CAAACCTGTT GTCCAAAAAC CAATTCCGAA AGATACATCA
    CTGCACAGTG CCCTCATGGA AGCTATTCAA ACAGGAGGGG GAAAAGAGAA ACTCAGAAAG
    GTTTCAGACA GCGCGCTAAA TGGCAATCAC AAGAAACCAT CATACAGTGA GCCAGAGAAT
    GAGCGGTCGG CCCTACTAGC GGCAATTAGA GGCCACAGTG GCACTTGCAA GCTGAAAAAA
    ATCTCCTCAT TGGCCTCCGA AGAGCTCCAG AGTTTTAGGA ATGCAGAACT GTCTTCACAG
    AAAGCAGAAG ACCCTCAGGA AGAGCAGCTC TGCATTCCAC CTGCCCCTGC CCAGCCACCA
    CCACCGCCTC CCCCTCAGCT GTCAACAGCA ACTTTCAAAT TCTCTGCCAC AACTACGGGT
    GACCCTGGAG AGGCCAGGCG AGCCCTGATG GAGGCCATTC GCTCTGGTGC TGGAGCAGCA
    AAGTTAAAAA AGGTCCCTCT GCTCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137802.1
    CDS367..4476
    Translation

    Target ORF information:

    RefSeq Version XM_015282316.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282316.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGACTC TCGTCCGCCC CACGGCCGGC AAGCCGCCCA GCGGGAGAAG AATGAAAGCT 
    CGTGCTCCTC CTCCACCAAA CCAACCTTCT GCAACCAGTA GAATTCACAG TGAGCACAAG
    TCACCTGCAG AGACAGCCGT AATTTCTGAT CAGAGCCTTG TGAGCATGAA GGAGAATATG
    ATAAATAGAA TGGTGGACTT CACAGTCATT TTACCCAGCG GGGTGGAACA GAAGAGCACA
    GTACAAGGAA GTAAAGCTGT GATGGATCTT TTGGTTGATC TATGCAGCCG GTATCACTTG
    AATCCATCCC AGTACAGTCT TGAATTGAAG TCTTTGGAAA CTCAACAACC TTTGAGTTAC
    AAGCCAAACA CTTTGATAGG AGCACTGGAC GTACAGACAG TACTCCTGAA AGAGAAGGTT
    CCTGAAGAGA AGATCAAGAG ACCTCTGCCG AGGGCTCCTG AGAAGTCTGT GAGGCTGGTG
    GTAAATTACC TGAAGACACA GAAGGCAGTG GTTCGAGTCA GCCCGGACGT GCCCCTTCAC
    AACATCATCC CAGCTATTTG TGAAAAGTGT GAGGTCAGTC AGGAGCACAT TGTTCTTCTG
    CGAGACAGCA TCACTGGCGA GGAGCTGGAG CTTACCAAAT CCTTGGAGGA GCTGGGAATA
    AGCGAGCTGT ACGCCTGGGA CCGAAAAAGA GAACCAAGTC GAAGTGTTTC ACTAAGCAAC
    GATGCAATAG AAAAAGAAAA GAAAGGATTT CTGGGGTTTT TCAAAGCTAA TAGAAGCAAC
    AGCAAGGGTT TTTCCACAGC ACCAAACTCC CCTTCGGTGA ATTCCCGTTC CAGTACCTTG
    GGTCCATCGC TGTCCCTTGG TAATATCTCA GGAATGACAG CAAACCCAGA AGTGAAAAAG
    CGCAGAGCAC CTCCTCCACC AGTTTTGACA CCTCCGTTGC AGAATATGGA GATGAGCTCC
    GCTCAGGAGA AGACAGCAGT ACAGATTTCA CAAGGAGCAT CTCTTAACGA TCTACAGAAA
    AAGAAGAGGC GTGCACCTCT TCCACCAACT CAGTCTGCTC CACCTACTCC AGCCATGCCA
    AATAGGAGGG AAGAGATGGA AGACAAGAGG AAGAGCACAA TGGGTGATGG ACGGCAGGTG
    CCACAAAAGC CTCCTAGGGG CCCCACTCGT GACCCACCCC AGTTAGTGAT CCCCCCACCC
    CCACCTTACC CTCCTCCTGA AAGAGACAAT ATGGATCCAG CAGTCTCTTA CAGAGAAGCA
    GATGTTACAG CACCAACAAA ACTGGTACCT AAACAGAGCC TGCTGCCTGC ACATGATAAT
    TTTTATGTAG TGGATGATAC AGTTATGGAG CTATCAGAGG TAGAAGAAAC AGCGTCAGAA
    AGCAGCTGCT TTGCATCTGA GGACACTACC GAAGACTCAG GTGTAGTGAG CTCCCCATCT
    GACATTGTGT CACTGGATTC ACAAAATGAC AGTATGAAGT TGAGAGACAT CAAGCTGGTG
    AATGGCTATG TGGACTCAGA CACGATCTAT GGTGCAGAGA CTTGCTCTGT GAAGAACGCG
    TACTGCGATG ATATGGGATA TGCCGGTACT CCACAGAGCA CTAAAAATGA AGAAATGGCT
    GCGGCTAAGC ATGAGAATGA GGACATATTC ATTGATGCAC AACTCCAGCA GACTTTGGCT
    GACCTGGATG AAGATTTAGA AGCTATGGAT GTTTTAAGTA ACTCTGACTC TGAGCACATG
    AGTGAAGTGT TGAGCCCACA TTCAGGAAAA GTTGAGGTCC CCCAAGATGT TGCTAGTTCT
    GTTCCAGTAA CAGTCATAGA TGATGCTCCA GAAGTTACCA TCTATAACTC AGCTGGAAAC
    CAAGAGACAA GGGGTTCACA GAATCTATCA GCTGACTCTG TTAATATGGG AAACTTCACA
    AATAGAAACA ACAATGCAGG TTCCTTTGAT AAGGGACACA CTGATGGTGG AGCTGTATGT
    ATACCCAAGG ACCTAATACA TCAGCAGCCA TCTGATAATG AGTTCAGTCA CATACCCGTG
    GAACAGAGGA GAGATATGTT TGAATCTCTC ACGTCCTCCT TTGAAAAAAG AAGTGAAAAG
    AATGTAAGAG TAGAAGCTCC GCCCAGACAC GTTATGAAGT CTGAGGAGTG TAAATCGAAG
    CTGACGTCAT CTCACTCCAA GACTGCGACT GAAATCAATA CAGCAAAAGA GAAAGTAAAT
    CCATTTAATG AATGTAGTAA GAGGGAGAGA CCTCTGAAAA AAAGTAAGTC AGAACTAGAA
    ATCAAAAGGC CACCAGCAAA AAAGATTGAA GTAGAAGAAG TTCCATCAAC ACCTCCGTGG
    TGTCATCGTG CCCAGAAATT GGAAACAAGT TATGAACCCA AAATGGGTTT GACGACCTTC
    AAAGTTGTCC CTCCCAAACC TGAAGTAAAA AATTTTGATA GAGGGCTTTC AGTCTCCACT
    GGTGCTATTA AGATTGATGA ACTAGGCAAC CTTATCAACC CAAAGCCTGT TGTCAGTAAG
    AACATACCAG GTACCTCTGC TGGTGAAAGT GAGGAAATTC CTCTTGGGAA AGTGAAGGCA
    TTCTGGAGGT CAAGTTCTAT GGAAAAACAA AGTGATGACT CTGCTGAGCA TCTTGCTAAA
    AAGCCAGCAG TTACCACAAA CTCTAAGTCC TTTGTTACAA AGCAAGAATG CAAACCTGTC
    TGTCTTGCAG CTCCAAAACC TGTACCACCA CAAACTGTTA TTTCCCAGGT AGGTGAAAAG
    CAGTCTGAGA ATGAGAAGAC CAAACCACCT CTTCCAGTTA CACAGTCTCA GGTAAAACCA
    TTAGTGGTCC CAGCCATTAA TAAGGACAAG CCGGAGCTTC CATTTCTAAA GCCTCACAGA
    AGAACTTCCA GTCAGTATGT GGCCTCTGCC ATTGCAAAAC ACATCAATCT GCCTACCTTT
    AAGACTGATT CTACAGATTT GCATGAGAAA GATGAAAATA AGCACGGGGC AGGAGAGGTT
    AAAACTGAAG CTGAAGTTTC CCCCAAAAGG TGTATTATTG TGGCCAAGTA CAGCCCTGTG
    GAAACAGAAT CAGCAGAAAC AAGAGAAAAA ATTTCAAGTA TTTTTACTTG TAGTCAAAAA
    GCATCCCACA GATTCACACC TGGTGATAAT TCTGGCATGG AAAACAAAGT AGTGAACATC
    AGGGCACCGA GTCAAGCAAC TCCTGCTAGT TTCTATAAAA AGAATGCCAG TGTCCCACTT
    ACTAAATCCT CTTCAAAGGA TAACAACAAA GCAGAAGATG ATCACAGTTT AAACAGCAAA
    CAAGGTACAG CAGAGAAAAA GATGCATCTT TTGTTTGACC AGAAATACGA GACTTTGCCC
    CGTAGTAATT CAGCCTCCTC TCTCAAGTCA CCTGATCTCC AAGGAGCCTC ATCTTCTTTC
    AGTTCATCTT TGCGCATCAC TAAATGGCCT CCTGCTGACA GCGTGCAAGT TAGTTCCCTG
    AAAGCATCGC CCGAGTTCAG TGGTGTGACA GCAAAGGCAG AATCAGAACA GAATGACAAA
    TCTGATGATT CGGCTGCTAA TGCTATTGGC GAATTGGATG TTAATGTGCA GACTAATATC
    TTCGGACCAA AGAAGAAGTT CAAACCTGTT GTCCAAAAAC CAATTCCGAA AGATACATCA
    CTGCACAGTG CCCTCATGGA AGCTATTCAA ACAGGAGGGG GAAAAGAGAA ACTCAGAAAG
    GTTTCAGACA GCGCGCTAAA TGGCAATCAC AAGAAACCAT CATACAGTGA GCCAGAGAAT
    GAGCGGTCGG CCCTACTAGC GGCAATTAGA GGCCACAGTG GCACTTGCAA GCTGAAAAAA
    ATCTCCTCAT TGGCCTCCGA AGAGCTCCAG AGTTTTAGGA ATGCAGAACT GTCTTCACAG
    AAAGCAGAAG ACCCTCAGGA AGAGCAGCTC TGCATTCCAC CTGCCCCTGC CCAGCCACCA
    CCACCGCCTC CCCCTCAGCT GTCAACAGCA ACTTTCAAAT TCTCTGCCAC AACTACGGGT
    GACCCTGGAG AGGCCAGGCG AGCCCTGATG GAGGCCATTC GCTCTGGTGC TGGAGCAGCA
    AAGTTAAAAA AGGTCCCTCT GCTCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32271
    Clone ID Related Accession (Same CDS sequence) XM_015282317.2 , XM_015282317.1
    Accession Version XM_015282317.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4068bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein cordon-bleu isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015282317.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGACTC TCGTCCGCCC CACGGCCGGC AAGCCGCCCA GCGGGAGAAG AATGAAAGCT 
    CGTGCTCCTC CTCCACCAAA CCAACCTTCT GCAACCAGTA GAATTCACAG TGAGCACAAG
    TCACCTGCAG AGACAGCCGT AATTTCTGAT CAGAGCCTTG TGAGCATGAA GGAGAATATG
    ATAAATAGAA TGGTGGACTT CACAGTCATT TTACCCAGCG GGGTGGAACA GAAGAGCACA
    GTACAAGGAA GTAAAGCTGT GATGGATCTT TTGGTTGATC TATGCAGCCG GTATCACTTG
    AATCCATCCC AGTACAGTCT TGAATTGAAG TCTTTGGAAA CTCAACAACC TTTGAGTTAC
    AAGCCAAACA CTTTGATAGG AGCACTGGAC GTACAGACAG TACTCCTGAA AGAGAAGGTT
    CCTGAAGAGA AGATCAAGAG ACCTCTGCCG AGGGCTCCTG AGAAGTCTGT GAGGCTGGTG
    GTAAATTACC TGAAGACACA GAAGGCAGTG GTTCGAGTCA GCCCGGACGT GCCCCTTCAC
    AACATCATCC CAGCTATTTG TGAAAAGTGT GAGGTCAGTC AGGAGCACAT TGTTCTTCTG
    CGAGACAGCA TCACTGGCGA GGAGCTGGAG CTTACCAAAT CCTTGGAGGA GCTGGGAATA
    AGCGAGCTGT ACGCCTGGGA CCGAAAAAGA GTTCCTCCAT CAAAAACTCA GTCTGACCCT
    TCTCTGAACT ACAGAGAACC AAGTCGAAGT GTTTCACTAA GCAACGATGC AATAGAAAAA
    GAAAAGAAAG GATTTCTGGG GTTTTTCAAA GCTAATAGAA GCAACAGCAA GACAGAAGAA
    CACTTGAGGA TGAACAGAGA TTATGGTGAG GAGGATGTTT TTAGTTCTAC ATCTACAAGT
    GAAGGAAGCT TGGATGGTTT TTCCACAGCA CCAAACTCCC CTTCGGTGAA TTCCCGTTCC
    AGTACCTTGG GTCCATCGCT GTCCCTTGGT AATATCTCAG GAATGACAGC AAACCCAGAA
    GTGAAAAAGC GCAGAGCACC TCCTCCACCA GTTTTGACAC CTCCGTTGCA GAATATGGAG
    ATGAGCTCCG CTCAGGAGAA GACAGCAGTA CAGATTTCAC AAGGAGCATC TCTTAACGAT
    CTACAGAAAA AGAAGAGGCG TGCACCTCTT CCACCAACTC AGTCTGCTCC ACCTACTCCA
    GCCATGCCAA ATAGGAGGGA AGAGATGGAA GACAAGAGGA AGAGCACAAT GGAGAGCCTG
    CTGCCTGCAC ATGATAATTT TTATGTAGTG GATGATACAG TTATGGAGCT ATCAGAGGTA
    GAAGAAACAG CGTCAGAAAG CAGCTGCTTT GCATCTGAGG ACACTACCGA AGACTCAGGT
    GTAGTGAGCT CCCCATCTGA CATTGTGTCA CTGGATTCAC AAAATGACAG TATGAAGTTG
    AGAGACATCA AGCTGGTGAA TGGCTATGTG GACTCAGACA CGATCTATGG TGCAGAGACT
    TGCTCTGTGA AGAACGCGTA CTGCGATGAT ATGGGATATG CCGGTACTCC ACAGAGCACT
    AAAAATGAAG AAATGGCTGC GGCTAAGCAT GAGAATGAGG ACATATTCAT TGATGCACAA
    CTCCAGCAGA CTTTGGCTGA CCTGGATGAA GATTTAGAAG CTATGGATGT TTTAAGTAAC
    TCTGACTCTG AGCACATGAG TGAAGTGTTG AGCCCACATT CAGGAAAAGT TGAGGTCCCC
    CAAGATGTTG CTAGTTCTGT TCCAGTAACA GTCATAGATG ATGCTCCAGA AGTTACCATC
    TATAACTCAG CTGGAAACCA AGAGACAAGG GGTTCACAGA ATCTATCAGC TGACTCTGTT
    AATATGGGAA ACTTCACAAA TAGAAACAAC AATGCAGGTT CCTTTGATAA GGGACACACT
    GATGGTGGAG CTGTATGTAT ACCCAAGGAC CTAATACATC AGCAGCCATC TGATAATGAG
    TTCAGTCACA TACCCGTGGA ACAGAGGAGA GATATGTTTG AATCTCTCAC GTCCTCCTTT
    GAAAAAAGAA GTGAAAAGAA TGTAAGAGTA GAAGCTCCGC CCAGACACGT TATGAAGTCT
    GAGGAGTGTA AATCGAAGCT GACGTCATCT CACTCCAAGA CTGCGACTGA AATCAATACA
    GCAAAAGAGA AAGTAAATCC ATTTAATGAA TGTAGTAAGA GGGAGAGACC TCTGAAAAAA
    AGTAAGTCAG AACTAGAAAT CAAAAGGCCA CCAGCAAAAA AGATTGAAGT AGAAGAAGTT
    CCATCAACAC CTCCGTGGTG TCATCGTGCC CAGAAATTGG AAACAAGTTA TGAACCCAAA
    ATGGGTTTGA CGACCTTCAA AGTTGTCCCT CCCAAACCTG AAGTAAAAAA TTTTGATAGA
    GGGCTTTCAG TCTCCACTGG TGCTATTAAG ATTGATGAAC TAGGCAACCT TATCAACCCA
    AAGCCTGTTG TCAGTAAGAA CATACCAGGT ACCTCTGCTG GTGAAAGTGA GGAAATTCCT
    CTTGGGAAAG TGAAGGCATT CTGGAGGTCA AGTTCTATGG AAAAACAAAG TGATGACTCT
    GCTGAGCATC TTGCTAAAAA GCCAGCAGTT ACCACAAACT CTAAGTCCTT TGTTACAAAG
    CAAGAATGCA AACCTGTCTG TCTTGCAGCT CCAAAACCTG TACCACCACA AACTGTTATT
    TCCCAGGTAG GTGAAAAGCA GTCTGAGAAT GAGAAGACCA AACCACCTCT TCCAGTTACA
    CAGTCTCAGG TAAAACCATT AGTGGTCCCA GCCATTAATA AGGACAAGCC GGAGCTTCCA
    TTTCTAAAGC CTCACAGAAG AACTTCCAGT CAGTATGTGG CCTCTGCCAT TGCAAAACAC
    ATCAATCTGC CTACCTTTAA GACTGATTCT ACAGATTTGC ATGAGAAAGA TGAAAATAAG
    CACGGGGCAG GAGAGGTTAA AACTGAAGCT GAAGTTTCCC CCAAAAGGTG TATTATTGTG
    GCCAAGTACA GCCCTGTGGA AACAGAATCA GCAGAAACAA GAGAAAAAAT TTCAAGTATT
    TTTACTTGTA GTCAAAAAGC ATCCCACAGA TTCACACCTG GTGATAATTC TGGCATGGAA
    AACAAAGTAG TGAACATCAG GGCACCGAGT CAAGCAACTC CTGCTAGTTT CTATAAAAAG
    AATGCCAGTG TCCCACTTAC TAAATCCTCT TCAAAGGATA ACAACAAAGC AGAAGATGAT
    CACAGTTTAA ACAGCAAACA AGGTACAGCA GAGAAAAAGA TGCATCTTTT GTTTGACCAG
    AAATACGAGA CTTTGCCCCG TAGTAATTCA GCCTCCTCTC TCAAGTCACC TGATCTCCAA
    GGAGCCTCAT CTTCTTTCAG TTCATCTTTG CGCATCACTA AATGGCCTCC TGCTGACAGC
    GTGCAAGTTA GTTCCCTGAA AGCATCGCCC GAGTTCAGTG GTGTGACAGC AAAGGCAGAA
    TCAGAACAGA ATGACAAATC TGATGATTCG GCTGCTAATG CTATTGGCGA ATTGGATGTT
    AATGTGCAGA CTAATATCTT CGGACCAAAG AAGAAGTTCA AACCTGTTGT CCAAAAACCA
    ATTCCGAAAG ATACATCACT GCACAGTGCC CTCATGGAAG CTATTCAAAC AGGAGGGGGA
    AAAGAGAAAC TCAGAAAGGT TTCAGACAGC GCGCTAAATG GCAATCACAA GAAACCATCA
    TACAGTGAGC CAGAGAATGA GCGGTCGGCC CTACTAGCGG CAATTAGAGG CCACAGTGGC
    ACTTGCAAGC TGAAAAAAAT CTCCTCATTG GCCTCCGAAG AGCTCCAGAG TTTTAGGAAT
    GCAGAACTGT CTTCACAGAA AGCAGAAGAC CCTCAGGAAG AGCAGCTCTG CATTCCACCT
    GCCCCTGCCC AGCCACCACC ACCGCCTCCC CCTCAGCTGT CAACAGCAAC TTTCAAATTC
    TCTGCCACAA CTACGGGTGA CCCTGGAGAG GCCAGGCGAG CCCTGATGGA GGCCATTCGC
    TCTGGTGCTG GAGCAGCAAA GTTAAAAAAG GTCCCTCTGC TCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137803.1
    CDS367..4434
    Translation

    Target ORF information:

    RefSeq Version XM_015282317.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282317.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGACTC TCGTCCGCCC CACGGCCGGC AAGCCGCCCA GCGGGAGAAG AATGAAAGCT 
    CGTGCTCCTC CTCCACCAAA CCAACCTTCT GCAACCAGTA GAATTCACAG TGAGCACAAG
    TCACCTGCAG AGACAGCCGT AATTTCTGAT CAGAGCCTTG TGAGCATGAA GGAGAATATG
    ATAAATAGAA TGGTGGACTT CACAGTCATT TTACCCAGCG GGGTGGAACA GAAGAGCACA
    GTACAAGGAA GTAAAGCTGT GATGGATCTT TTGGTTGATC TATGCAGCCG GTATCACTTG
    AATCCATCCC AGTACAGTCT TGAATTGAAG TCTTTGGAAA CTCAACAACC TTTGAGTTAC
    AAGCCAAACA CTTTGATAGG AGCACTGGAC GTACAGACAG TACTCCTGAA AGAGAAGGTT
    CCTGAAGAGA AGATCAAGAG ACCTCTGCCG AGGGCTCCTG AGAAGTCTGT GAGGCTGGTG
    GTAAATTACC TGAAGACACA GAAGGCAGTG GTTCGAGTCA GCCCGGACGT GCCCCTTCAC
    AACATCATCC CAGCTATTTG TGAAAAGTGT GAGGTCAGTC AGGAGCACAT TGTTCTTCTG
    CGAGACAGCA TCACTGGCGA GGAGCTGGAG CTTACCAAAT CCTTGGAGGA GCTGGGAATA
    AGCGAGCTGT ACGCCTGGGA CCGAAAAAGA GTTCCTCCAT CAAAAACTCA GTCTGACCCT
    TCTCTGAACT ACAGAGAACC AAGTCGAAGT GTTTCACTAA GCAACGATGC AATAGAAAAA
    GAAAAGAAAG GATTTCTGGG GTTTTTCAAA GCTAATAGAA GCAACAGCAA GACAGAAGAA
    CACTTGAGGA TGAACAGAGA TTATGGTGAG GAGGATGTTT TTAGTTCTAC ATCTACAAGT
    GAAGGAAGCT TGGATGGTTT TTCCACAGCA CCAAACTCCC CTTCGGTGAA TTCCCGTTCC
    AGTACCTTGG GTCCATCGCT GTCCCTTGGT AATATCTCAG GAATGACAGC AAACCCAGAA
    GTGAAAAAGC GCAGAGCACC TCCTCCACCA GTTTTGACAC CTCCGTTGCA GAATATGGAG
    ATGAGCTCCG CTCAGGAGAA GACAGCAGTA CAGATTTCAC AAGGAGCATC TCTTAACGAT
    CTACAGAAAA AGAAGAGGCG TGCACCTCTT CCACCAACTC AGTCTGCTCC ACCTACTCCA
    GCCATGCCAA ATAGGAGGGA AGAGATGGAA GACAAGAGGA AGAGCACAAT GGAGAGCCTG
    CTGCCTGCAC ATGATAATTT TTATGTAGTG GATGATACAG TTATGGAGCT ATCAGAGGTA
    GAAGAAACAG CGTCAGAAAG CAGCTGCTTT GCATCTGAGG ACACTACCGA AGACTCAGGT
    GTAGTGAGCT CCCCATCTGA CATTGTGTCA CTGGATTCAC AAAATGACAG TATGAAGTTG
    AGAGACATCA AGCTGGTGAA TGGCTATGTG GACTCAGACA CGATCTATGG TGCAGAGACT
    TGCTCTGTGA AGAACGCGTA CTGCGATGAT ATGGGATATG CCGGTACTCC ACAGAGCACT
    AAAAATGAAG AAATGGCTGC GGCTAAGCAT GAGAATGAGG ACATATTCAT TGATGCACAA
    CTCCAGCAGA CTTTGGCTGA CCTGGATGAA GATTTAGAAG CTATGGATGT TTTAAGTAAC
    TCTGACTCTG AGCACATGAG TGAAGTGTTG AGCCCACATT CAGGAAAAGT TGAGGTCCCC
    CAAGATGTTG CTAGTTCTGT TCCAGTAACA GTCATAGATG ATGCTCCAGA AGTTACCATC
    TATAACTCAG CTGGAAACCA AGAGACAAGG GGTTCACAGA ATCTATCAGC TGACTCTGTT
    AATATGGGAA ACTTCACAAA TAGAAACAAC AATGCAGGTT CCTTTGATAA GGGACACACT
    GATGGTGGAG CTGTATGTAT ACCCAAGGAC CTAATACATC AGCAGCCATC TGATAATGAG
    TTCAGTCACA TACCCGTGGA ACAGAGGAGA GATATGTTTG AATCTCTCAC GTCCTCCTTT
    GAAAAAAGAA GTGAAAAGAA TGTAAGAGTA GAAGCTCCGC CCAGACACGT TATGAAGTCT
    GAGGAGTGTA AATCGAAGCT GACGTCATCT CACTCCAAGA CTGCGACTGA AATCAATACA
    GCAAAAGAGA AAGTAAATCC ATTTAATGAA TGTAGTAAGA GGGAGAGACC TCTGAAAAAA
    AGTAAGTCAG AACTAGAAAT CAAAAGGCCA CCAGCAAAAA AGATTGAAGT AGAAGAAGTT
    CCATCAACAC CTCCGTGGTG TCATCGTGCC CAGAAATTGG AAACAAGTTA TGAACCCAAA
    ATGGGTTTGA CGACCTTCAA AGTTGTCCCT CCCAAACCTG AAGTAAAAAA TTTTGATAGA
    GGGCTTTCAG TCTCCACTGG TGCTATTAAG ATTGATGAAC TAGGCAACCT TATCAACCCA
    AAGCCTGTTG TCAGTAAGAA CATACCAGGT ACCTCTGCTG GTGAAAGTGA GGAAATTCCT
    CTTGGGAAAG TGAAGGCATT CTGGAGGTCA AGTTCTATGG AAAAACAAAG TGATGACTCT
    GCTGAGCATC TTGCTAAAAA GCCAGCAGTT ACCACAAACT CTAAGTCCTT TGTTACAAAG
    CAAGAATGCA AACCTGTCTG TCTTGCAGCT CCAAAACCTG TACCACCACA AACTGTTATT
    TCCCAGGTAG GTGAAAAGCA GTCTGAGAAT GAGAAGACCA AACCACCTCT TCCAGTTACA
    CAGTCTCAGG TAAAACCATT AGTGGTCCCA GCCATTAATA AGGACAAGCC GGAGCTTCCA
    TTTCTAAAGC CTCACAGAAG AACTTCCAGT CAGTATGTGG CCTCTGCCAT TGCAAAACAC
    ATCAATCTGC CTACCTTTAA GACTGATTCT ACAGATTTGC ATGAGAAAGA TGAAAATAAG
    CACGGGGCAG GAGAGGTTAA AACTGAAGCT GAAGTTTCCC CCAAAAGGTG TATTATTGTG
    GCCAAGTACA GCCCTGTGGA AACAGAATCA GCAGAAACAA GAGAAAAAAT TTCAAGTATT
    TTTACTTGTA GTCAAAAAGC ATCCCACAGA TTCACACCTG GTGATAATTC TGGCATGGAA
    AACAAAGTAG TGAACATCAG GGCACCGAGT CAAGCAACTC CTGCTAGTTT CTATAAAAAG
    AATGCCAGTG TCCCACTTAC TAAATCCTCT TCAAAGGATA ACAACAAAGC AGAAGATGAT
    CACAGTTTAA ACAGCAAACA AGGTACAGCA GAGAAAAAGA TGCATCTTTT GTTTGACCAG
    AAATACGAGA CTTTGCCCCG TAGTAATTCA GCCTCCTCTC TCAAGTCACC TGATCTCCAA
    GGAGCCTCAT CTTCTTTCAG TTCATCTTTG CGCATCACTA AATGGCCTCC TGCTGACAGC
    GTGCAAGTTA GTTCCCTGAA AGCATCGCCC GAGTTCAGTG GTGTGACAGC AAAGGCAGAA
    TCAGAACAGA ATGACAAATC TGATGATTCG GCTGCTAATG CTATTGGCGA ATTGGATGTT
    AATGTGCAGA CTAATATCTT CGGACCAAAG AAGAAGTTCA AACCTGTTGT CCAAAAACCA
    ATTCCGAAAG ATACATCACT GCACAGTGCC CTCATGGAAG CTATTCAAAC AGGAGGGGGA
    AAAGAGAAAC TCAGAAAGGT TTCAGACAGC GCGCTAAATG GCAATCACAA GAAACCATCA
    TACAGTGAGC CAGAGAATGA GCGGTCGGCC CTACTAGCGG CAATTAGAGG CCACAGTGGC
    ACTTGCAAGC TGAAAAAAAT CTCCTCATTG GCCTCCGAAG AGCTCCAGAG TTTTAGGAAT
    GCAGAACTGT CTTCACAGAA AGCAGAAGAC CCTCAGGAAG AGCAGCTCTG CATTCCACCT
    GCCCCTGCCC AGCCACCACC ACCGCCTCCC CCTCAGCTGT CAACAGCAAC TTTCAAATTC
    TCTGCCACAA CTACGGGTGA CCCTGGAGAG GCCAGGCGAG CCCTGATGGA GGCCATTCGC
    TCTGGTGCTG GAGCAGCAAA GTTAAAAAAG GTCCCTCTGC TCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32271
    Clone ID Related Accession (Same CDS sequence) XM_015282317.2 , XM_015282317.1
    Accession Version XM_015282317.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4068bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product protein cordon-bleu isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455789.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGACTC TCGTCCGCCC CACGGCCGGC AAGCCGCCCA GCGGGAGAAG AATGAAAGCT 
    CGTGCTCCTC CTCCACCAAA CCAACCTTCT GCAACCAGTA GAATTCACAG TGAGCACAAG
    TCACCTGCAG AGACAGCCGT AATTTCTGAT CAGAGCCTTG TGAGCATGAA GGAGAATATG
    ATAAATAGAA TGGTGGACTT CACAGTCATT TTACCCAGCG GGGTGGAACA GAAGAGCACA
    GTACAAGGAA GTAAAGCTGT GATGGATCTT TTGGTTGATC TATGCAGCCG GTATCACTTG
    AATCCATCCC AGTACAGTCT TGAATTGAAG TCTTTGGAAA CTCAACAACC TTTGAGTTAC
    AAGCCAAACA CTTTGATAGG AGCACTGGAC GTACAGACAG TACTCCTGAA AGAGAAGGTT
    CCTGAAGAGA AGATCAAGAG ACCTCTGCCG AGGGCTCCTG AGAAGTCTGT GAGGCTGGTG
    GTAAATTACC TGAAGACACA GAAGGCAGTG GTTCGAGTCA GCCCGGACGT GCCCCTTCAC
    AACATCATCC CAGCTATTTG TGAAAAGTGT GAGGTCAGTC AGGAGCACAT TGTTCTTCTG
    CGAGACAGCA TCACTGGCGA GGAGCTGGAG CTTACCAAAT CCTTGGAGGA GCTGGGAATA
    AGCGAGCTGT ACGCCTGGGA CCGAAAAAGA GTTCCTCCAT CAAAAACTCA GTCTGACCCT
    TCTCTGAACT ACAGAGAACC AAGTCGAAGT GTTTCACTAA GCAACGATGC AATAGAAAAA
    GAAAAGAAAG GATTTCTGGG GTTTTTCAAA GCTAATAGAA GCAACAGCAA GACAGAAGAA
    CACTTGAGGA TGAACAGAGA TTATGGTGAG GAGGATGTTT TTAGTTCTAC ATCTACAAGT
    GAAGGAAGCT TGGATGGTTT TTCCACAGCA CCAAACTCCC CTTCGGTGAA TTCCCGTTCC
    AGTACCTTGG GTCCATCGCT GTCCCTTGGT AATATCTCAG GAATGACAGC AAACCCAGAA
    GTGAAAAAGC GCAGAGCACC TCCTCCACCA GTTTTGACAC CTCCGTTGCA GAATATGGAG
    ATGAGCTCCG CTCAGGAGAA GACAGCAGTA CAGATTTCAC AAGGAGCATC TCTTAACGAT
    CTACAGAAAA AGAAGAGGCG TGCACCTCTT CCACCAACTC AGTCTGCTCC ACCTACTCCA
    GCCATGCCAA ATAGGAGGGA AGAGATGGAA GACAAGAGGA AGAGCACAAT GGAGAGCCTG
    CTGCCTGCAC ATGATAATTT TTATGTAGTG GATGATACAG TTATGGAGCT ATCAGAGGTA
    GAAGAAACAG CGTCAGAAAG CAGCTGCTTT GCATCTGAGG ACACTACCGA AGACTCAGGT
    GTAGTGAGCT CCCCATCTGA CATTGTGTCA CTGGATTCAC AAAATGACAG TATGAAGTTG
    AGAGACATCA AGCTGGTGAA TGGCTATGTG GACTCAGACA CGATCTATGG TGCAGAGACT
    TGCTCTGTGA AGAACGCGTA CTGCGATGAT ATGGGATATG CCGGTACTCC ACAGAGCACT
    AAAAATGAAG AAATGGCTGC GGCTAAGCAT GAGAATGAGG ACATATTCAT TGATGCACAA
    CTCCAGCAGA CTTTGGCTGA CCTGGATGAA GATTTAGAAG CTATGGATGT TTTAAGTAAC
    TCTGACTCTG AGCACATGAG TGAAGTGTTG AGCCCACATT CAGGAAAAGT TGAGGTCCCC
    CAAGATGTTG CTAGTTCTGT TCCAGTAACA GTCATAGATG ATGCTCCAGA AGTTACCATC
    TATAACTCAG CTGGAAACCA AGAGACAAGG GGTTCACAGA ATCTATCAGC TGACTCTGTT
    AATATGGGAA ACTTCACAAA TAGAAACAAC AATGCAGGTT CCTTTGATAA GGGACACACT
    GATGGTGGAG CTGTATGTAT ACCCAAGGAC CTAATACATC AGCAGCCATC TGATAATGAG
    TTCAGTCACA TACCCGTGGA ACAGAGGAGA GATATGTTTG AATCTCTCAC GTCCTCCTTT
    GAAAAAAGAA GTGAAAAGAA TGTAAGAGTA GAAGCTCCGC CCAGACACGT TATGAAGTCT
    GAGGAGTGTA AATCGAAGCT GACGTCATCT CACTCCAAGA CTGCGACTGA AATCAATACA
    GCAAAAGAGA AAGTAAATCC ATTTAATGAA TGTAGTAAGA GGGAGAGACC TCTGAAAAAA
    AGTAAGTCAG AACTAGAAAT CAAAAGGCCA CCAGCAAAAA AGATTGAAGT AGAAGAAGTT
    CCATCAACAC CTCCGTGGTG TCATCGTGCC CAGAAATTGG AAACAAGTTA TGAACCCAAA
    ATGGGTTTGA CGACCTTCAA AGTTGTCCCT CCCAAACCTG AAGTAAAAAA TTTTGATAGA
    GGGCTTTCAG TCTCCACTGG TGCTATTAAG ATTGATGAAC TAGGCAACCT TATCAACCCA
    AAGCCTGTTG TCAGTAAGAA CATACCAGGT ACCTCTGCTG GTGAAAGTGA GGAAATTCCT
    CTTGGGAAAG TGAAGGCATT CTGGAGGTCA AGTTCTATGG AAAAACAAAG TGATGACTCT
    GCTGAGCATC TTGCTAAAAA GCCAGCAGTT ACCACAAACT CTAAGTCCTT TGTTACAAAG
    CAAGAATGCA AACCTGTCTG TCTTGCAGCT CCAAAACCTG TACCACCACA AACTGTTATT
    TCCCAGGTAG GTGAAAAGCA GTCTGAGAAT GAGAAGACCA AACCACCTCT TCCAGTTACA
    CAGTCTCAGG TAAAACCATT AGTGGTCCCA GCCATTAATA AGGACAAGCC GGAGCTTCCA
    TTTCTAAAGC CTCACAGAAG AACTTCCAGT CAGTATGTGG CCTCTGCCAT TGCAAAACAC
    ATCAATCTGC CTACCTTTAA GACTGATTCT ACAGATTTGC ATGAGAAAGA TGAAAATAAG
    CACGGGGCAG GAGAGGTTAA AACTGAAGCT GAAGTTTCCC CCAAAAGGTG TATTATTGTG
    GCCAAGTACA GCCCTGTGGA AACAGAATCA GCAGAAACAA GAGAAAAAAT TTCAAGTATT
    TTTACTTGTA GTCAAAAAGC ATCCCACAGA TTCACACCTG GTGATAATTC TGGCATGGAA
    AACAAAGTAG TGAACATCAG GGCACCGAGT CAAGCAACTC CTGCTAGTTT CTATAAAAAG
    AATGCCAGTG TCCCACTTAC TAAATCCTCT TCAAAGGATA ACAACAAAGC AGAAGATGAT
    CACAGTTTAA ACAGCAAACA AGGTACAGCA GAGAAAAAGA TGCATCTTTT GTTTGACCAG
    AAATACGAGA CTTTGCCCCG TAGTAATTCA GCCTCCTCTC TCAAGTCACC TGATCTCCAA
    GGAGCCTCAT CTTCTTTCAG TTCATCTTTG CGCATCACTA AATGGCCTCC TGCTGACAGC
    GTGCAAGTTA GTTCCCTGAA AGCATCGCCC GAGTTCAGTG GTGTGACAGC AAAGGCAGAA
    TCAGAACAGA ATGACAAATC TGATGATTCG GCTGCTAATG CTATTGGCGA ATTGGATGTT
    AATGTGCAGA CTAATATCTT CGGACCAAAG AAGAAGTTCA AACCTGTTGT CCAAAAACCA
    ATTCCGAAAG ATACATCACT GCACAGTGCC CTCATGGAAG CTATTCAAAC AGGAGGGGGA
    AAAGAGAAAC TCAGAAAGGT TTCAGACAGC GCGCTAAATG GCAATCACAA GAAACCATCA
    TACAGTGAGC CAGAGAATGA GCGGTCGGCC CTACTAGCGG CAATTAGAGG CCACAGTGGC
    ACTTGCAAGC TGAAAAAAAT CTCCTCATTG GCCTCCGAAG AGCTCCAGAG TTTTAGGAAT
    GCAGAACTGT CTTCACAGAA AGCAGAAGAC CCTCAGGAAG AGCAGCTCTG CATTCCACCT
    GCCCCTGCCC AGCCACCACC ACCGCCTCCC CCTCAGCTGT CAACAGCAAC TTTCAAATTC
    TCTGCCACAA CTACGGGTGA CCCTGGAGAG GCCAGGCGAG CCCTGATGGA GGCCATTCGC
    TCTGGTGCTG GAGCAGCAAA GTTAAAAAAG GTCCCTCTGC TCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137803.1
    CDS194..4261
    Translation

    Target ORF information:

    RefSeq Version XM_015282317.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282317.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGACTC TCGTCCGCCC CACGGCCGGC AAGCCGCCCA GCGGGAGAAG AATGAAAGCT 
    CGTGCTCCTC CTCCACCAAA CCAACCTTCT GCAACCAGTA GAATTCACAG TGAGCACAAG
    TCACCTGCAG AGACAGCCGT AATTTCTGAT CAGAGCCTTG TGAGCATGAA GGAGAATATG
    ATAAATAGAA TGGTGGACTT CACAGTCATT TTACCCAGCG GGGTGGAACA GAAGAGCACA
    GTACAAGGAA GTAAAGCTGT GATGGATCTT TTGGTTGATC TATGCAGCCG GTATCACTTG
    AATCCATCCC AGTACAGTCT TGAATTGAAG TCTTTGGAAA CTCAACAACC TTTGAGTTAC
    AAGCCAAACA CTTTGATAGG AGCACTGGAC GTACAGACAG TACTCCTGAA AGAGAAGGTT
    CCTGAAGAGA AGATCAAGAG ACCTCTGCCG AGGGCTCCTG AGAAGTCTGT GAGGCTGGTG
    GTAAATTACC TGAAGACACA GAAGGCAGTG GTTCGAGTCA GCCCGGACGT GCCCCTTCAC
    AACATCATCC CAGCTATTTG TGAAAAGTGT GAGGTCAGTC AGGAGCACAT TGTTCTTCTG
    CGAGACAGCA TCACTGGCGA GGAGCTGGAG CTTACCAAAT CCTTGGAGGA GCTGGGAATA
    AGCGAGCTGT ACGCCTGGGA CCGAAAAAGA GTTCCTCCAT CAAAAACTCA GTCTGACCCT
    TCTCTGAACT ACAGAGAACC AAGTCGAAGT GTTTCACTAA GCAACGATGC AATAGAAAAA
    GAAAAGAAAG GATTTCTGGG GTTTTTCAAA GCTAATAGAA GCAACAGCAA GACAGAAGAA
    CACTTGAGGA TGAACAGAGA TTATGGTGAG GAGGATGTTT TTAGTTCTAC ATCTACAAGT
    GAAGGAAGCT TGGATGGTTT TTCCACAGCA CCAAACTCCC CTTCGGTGAA TTCCCGTTCC
    AGTACCTTGG GTCCATCGCT GTCCCTTGGT AATATCTCAG GAATGACAGC AAACCCAGAA
    GTGAAAAAGC GCAGAGCACC TCCTCCACCA GTTTTGACAC CTCCGTTGCA GAATATGGAG
    ATGAGCTCCG CTCAGGAGAA GACAGCAGTA CAGATTTCAC AAGGAGCATC TCTTAACGAT
    CTACAGAAAA AGAAGAGGCG TGCACCTCTT CCACCAACTC AGTCTGCTCC ACCTACTCCA
    GCCATGCCAA ATAGGAGGGA AGAGATGGAA GACAAGAGGA AGAGCACAAT GGAGAGCCTG
    CTGCCTGCAC ATGATAATTT TTATGTAGTG GATGATACAG TTATGGAGCT ATCAGAGGTA
    GAAGAAACAG CGTCAGAAAG CAGCTGCTTT GCATCTGAGG ACACTACCGA AGACTCAGGT
    GTAGTGAGCT CCCCATCTGA CATTGTGTCA CTGGATTCAC AAAATGACAG TATGAAGTTG
    AGAGACATCA AGCTGGTGAA TGGCTATGTG GACTCAGACA CGATCTATGG TGCAGAGACT
    TGCTCTGTGA AGAACGCGTA CTGCGATGAT ATGGGATATG CCGGTACTCC ACAGAGCACT
    AAAAATGAAG AAATGGCTGC GGCTAAGCAT GAGAATGAGG ACATATTCAT TGATGCACAA
    CTCCAGCAGA CTTTGGCTGA CCTGGATGAA GATTTAGAAG CTATGGATGT TTTAAGTAAC
    TCTGACTCTG AGCACATGAG TGAAGTGTTG AGCCCACATT CAGGAAAAGT TGAGGTCCCC
    CAAGATGTTG CTAGTTCTGT TCCAGTAACA GTCATAGATG ATGCTCCAGA AGTTACCATC
    TATAACTCAG CTGGAAACCA AGAGACAAGG GGTTCACAGA ATCTATCAGC TGACTCTGTT
    AATATGGGAA ACTTCACAAA TAGAAACAAC AATGCAGGTT CCTTTGATAA GGGACACACT
    GATGGTGGAG CTGTATGTAT ACCCAAGGAC CTAATACATC AGCAGCCATC TGATAATGAG
    TTCAGTCACA TACCCGTGGA ACAGAGGAGA GATATGTTTG AATCTCTCAC GTCCTCCTTT
    GAAAAAAGAA GTGAAAAGAA TGTAAGAGTA GAAGCTCCGC CCAGACACGT TATGAAGTCT
    GAGGAGTGTA AATCGAAGCT GACGTCATCT CACTCCAAGA CTGCGACTGA AATCAATACA
    GCAAAAGAGA AAGTAAATCC ATTTAATGAA TGTAGTAAGA GGGAGAGACC TCTGAAAAAA
    AGTAAGTCAG AACTAGAAAT CAAAAGGCCA CCAGCAAAAA AGATTGAAGT AGAAGAAGTT
    CCATCAACAC CTCCGTGGTG TCATCGTGCC CAGAAATTGG AAACAAGTTA TGAACCCAAA
    ATGGGTTTGA CGACCTTCAA AGTTGTCCCT CCCAAACCTG AAGTAAAAAA TTTTGATAGA
    GGGCTTTCAG TCTCCACTGG TGCTATTAAG ATTGATGAAC TAGGCAACCT TATCAACCCA
    AAGCCTGTTG TCAGTAAGAA CATACCAGGT ACCTCTGCTG GTGAAAGTGA GGAAATTCCT
    CTTGGGAAAG TGAAGGCATT CTGGAGGTCA AGTTCTATGG AAAAACAAAG TGATGACTCT
    GCTGAGCATC TTGCTAAAAA GCCAGCAGTT ACCACAAACT CTAAGTCCTT TGTTACAAAG
    CAAGAATGCA AACCTGTCTG TCTTGCAGCT CCAAAACCTG TACCACCACA AACTGTTATT
    TCCCAGGTAG GTGAAAAGCA GTCTGAGAAT GAGAAGACCA AACCACCTCT TCCAGTTACA
    CAGTCTCAGG TAAAACCATT AGTGGTCCCA GCCATTAATA AGGACAAGCC GGAGCTTCCA
    TTTCTAAAGC CTCACAGAAG AACTTCCAGT CAGTATGTGG CCTCTGCCAT TGCAAAACAC
    ATCAATCTGC CTACCTTTAA GACTGATTCT ACAGATTTGC ATGAGAAAGA TGAAAATAAG
    CACGGGGCAG GAGAGGTTAA AACTGAAGCT GAAGTTTCCC CCAAAAGGTG TATTATTGTG
    GCCAAGTACA GCCCTGTGGA AACAGAATCA GCAGAAACAA GAGAAAAAAT TTCAAGTATT
    TTTACTTGTA GTCAAAAAGC ATCCCACAGA TTCACACCTG GTGATAATTC TGGCATGGAA
    AACAAAGTAG TGAACATCAG GGCACCGAGT CAAGCAACTC CTGCTAGTTT CTATAAAAAG
    AATGCCAGTG TCCCACTTAC TAAATCCTCT TCAAAGGATA ACAACAAAGC AGAAGATGAT
    CACAGTTTAA ACAGCAAACA AGGTACAGCA GAGAAAAAGA TGCATCTTTT GTTTGACCAG
    AAATACGAGA CTTTGCCCCG TAGTAATTCA GCCTCCTCTC TCAAGTCACC TGATCTCCAA
    GGAGCCTCAT CTTCTTTCAG TTCATCTTTG CGCATCACTA AATGGCCTCC TGCTGACAGC
    GTGCAAGTTA GTTCCCTGAA AGCATCGCCC GAGTTCAGTG GTGTGACAGC AAAGGCAGAA
    TCAGAACAGA ATGACAAATC TGATGATTCG GCTGCTAATG CTATTGGCGA ATTGGATGTT
    AATGTGCAGA CTAATATCTT CGGACCAAAG AAGAAGTTCA AACCTGTTGT CCAAAAACCA
    ATTCCGAAAG ATACATCACT GCACAGTGCC CTCATGGAAG CTATTCAAAC AGGAGGGGGA
    AAAGAGAAAC TCAGAAAGGT TTCAGACAGC GCGCTAAATG GCAATCACAA GAAACCATCA
    TACAGTGAGC CAGAGAATGA GCGGTCGGCC CTACTAGCGG CAATTAGAGG CCACAGTGGC
    ACTTGCAAGC TGAAAAAAAT CTCCTCATTG GCCTCCGAAG AGCTCCAGAG TTTTAGGAAT
    GCAGAACTGT CTTCACAGAA AGCAGAAGAC CCTCAGGAAG AGCAGCTCTG CATTCCACCT
    GCCCCTGCCC AGCCACCACC ACCGCCTCCC CCTCAGCTGT CAACAGCAAC TTTCAAATTC
    TCTGCCACAA CTACGGGTGA CCCTGGAGAG GCCAGGCGAG CCCTGATGGA GGCCATTCGC
    TCTGGTGCTG GAGCAGCAAA GTTAAAAAAG GTCCCTCTGC TCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32272
    Clone ID Related Accession (Same CDS sequence) XM_015282318.1 , XM_015282318.2 , XM_015282319.1 , XM_015282319.2
    Accession Version XM_015282318.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3390bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product protein cordon-bleu isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455789.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAACAGAG ATTATGGTGA GGAGGATGTT TTTAGTTCTA CATCTACAAG TGAAGGAAGC 
    TTGGATGGTT TTTCCACAGC ACCAAACTCC CCTTCGGTGA ATTCCCGTTC CAGTACCTTG
    GGTCCATCGC TGTCCCTTGG TAATATCTCA GGAATGACAG CAAACCCAGA AGTGAAAAAG
    CGCAGAGCAC CTCCTCCACC AGTTTTGACA CCTCCGTTGC AGAATATGGA GATGAGCTCC
    GCTCAGGAGA AGACAGCAGT ACAGATTTCA CAAGGAGCAT CTCTTAACGA TCTACAGAAA
    AAGAAGAGGC GTGCACCTCT TCCACCAACT CAGTCTGCTC CACCTACTCC AGCCATGCCA
    AATAGGAGGG AAGAGATGGA AGACAAGAGG AAGAGCACAA TGGGTGATGG ACGGCAGGTG
    CCACAAAAGC CTCCTAGGGG CCCCACTCGT GACCCACCCC AGTTAGTGAT CCCCCCACCC
    CCACCTTACC CTCCTCCTGA AAGAGACAAT ATGGATCCAG CAGTCTCTTA CAGAGAAGCA
    GATGTTACAG CACCAACAAA ACTGGTACCT AAACAGAGCC TGCTGCCTGC ACATGATAAT
    TTTTATGTAG TGGATGATAC AGTTATGGAG CTATCAGAGG TAGAAGAAAC AGCGTCAGAA
    AGCAGCTGCT TTGCATCTGA GGACACTACC GAAGACTCAG GTGTAGTGAG CTCCCCATCT
    GACATTGTGT CACTGGATTC ACAAAATGAC AGTATGAAGT TGAGAGACAT CAAGCTGGTG
    AATGGCTATG TGGACTCAGA CACGATCTAT GGTGCAGAGA CTTGCTCTGT GAAGAACGCG
    TACTGCGATG ATATGGGATA TGCCGGTACT CCACAGAGCA CTAAAAATGA AGAAATGGCT
    GCGGCTAAGC ATGAGAATGA GGACATATTC ATTGATGCAC AACTCCAGCA GACTTTGGCT
    GACCTGGATG AAGATTTAGA AGCTATGGAT GTTTTAAGTA ACTCTGACTC TGAGCACATG
    AGTGAAGTGT TGAGCCCACA TTCAGGAAAA GTTGAGGTCC CCCAAGATGT TGCTAGTTCT
    GTTCCAGTAA CAGTCATAGA TGATGCTCCA GAAGTTACCA TCTATAACTC AGCTGGAAAC
    CAAGAGACAA GGGGTTCACA GAATCTATCA GCTGACTCTG TTAATATGGG AAACTTCACA
    AATAGAAACA ACAATGCAGG TTCCTTTGAT AAGGGACACA CTGATGGTGG AGCTGTATGT
    ATACCCAAGG ACCTAATACA TCAGCAGCCA TCTGATAATG AGTTCAGTCA CATACCCGTG
    GAACAGAGGA GAGATATGTT TGAATCTCTC ACGTCCTCCT TTGAAAAAAG AAGTGAAAAG
    AATGTAAGAG TAGAAGCTCC GCCCAGACAC GTTATGAAGT CTGAGGAGTG TAAATCGAAG
    CTGACGTCAT CTCACTCCAA GACTGCGACT GAAATCAATA CAGCAAAAGA GAAAGTAAAT
    CCATTTAATG AATGTAGTAA GAGGGAGAGA CCTCTGAAAA AAAGTAAGTC AGAACTAGAA
    ATCAAAAGGC CACCAGCAAA AAAGATTGAA GTAGAAGAAG TTCCATCAAC ACCTCCGTGG
    TGTCATCGTG CCCAGAAATT GGAAACAAGT TATGAACCCA AAATGGGTTT GACGACCTTC
    AAAGTTGTCC CTCCCAAACC TGAAGTAAAA AATTTTGATA GAGGGCTTTC AGTCTCCACT
    GGTGCTATTA AGATTGATGA ACTAGGCAAC CTTATCAACC CAAAGCCTGT TGTCAGTAAG
    AACATACCAG GTACCTCTGC TGGTGAAAGT GAGGAAATTC CTCTTGGGAA AGTGAAGGCA
    TTCTGGAGGT CAAGTTCTAT GGAAAAACAA AGTGATGACT CTGCTGAGCA TCTTGCTAAA
    AAGCCAGCAG TTACCACAAA CTCTAAGTCC TTTGTTACAA AGCAAGAATG CAAACCTGTC
    TGTCTTGCAG CTCCAAAACC TGTACCACCA CAAACTGTTA TTTCCCAGGT AGGTGAAAAG
    CAGTCTGAGA ATGAGAAGAC CAAACCACCT CTTCCAGTTA CACAGTCTCA GGTAAAACCA
    TTAGTGGTCC CAGCCATTAA TAAGGACAAG CCGGAGCTTC CATTTCTAAA GCCTCACAGA
    AGAACTTCCA GTCAGTATGT GGCCTCTGCC ATTGCAAAAC ACATCAATCT GCCTACCTTT
    AAGACTGATT CTACAGATTT GCATGAGAAA GATGAAAATA AGCACGGGGC AGGAGAGGTT
    AAAACTGAAG CTGAAGTTTC CCCCAAAAGG TGTATTATTG TGGCCAAGTA CAGCCCTGTG
    GAAACAGAAT CAGCAGAAAC AAGAGAAAAA ATTTCAAGTA TTTTTACTTG TAGTCAAAAA
    GCATCCCACA GATTCACACC TGGTGATAAT TCTGGCATGG AAAACAAAGT AGTGAACATC
    AGGGCACCGA GTCAAGCAAC TCCTGCTAGT TTCTATAAAA AGAATGCCAG TGTCCCACTT
    ACTAAATCCT CTTCAAAGGA TAACAACAAA GCAGAAGATG ATCACAGTTT AAACAGCAAA
    CAAGGTACAG CAGAGAAAAA GATGCATCTT TTGTTTGACC AGAAATACGA GACTTTGCCC
    CGTAGTAATT CAGCCTCCTC TCTCAAGTCA CCTGATCTCC AAGGAGCCTC ATCTTCTTTC
    AGTTCATCTT TGCGCATCAC TAAATGGCCT CCTGCTGACA GCGTGCAAGT TAGTTCCCTG
    AAAGCATCGC CCGAGTTCAG TGGTGTGACA GCAAAGGCAG AATCAGAACA GAATGACAAA
    TCTGATGATT CGGCTGCTAA TGCTATTGGC GAATTGGATG TTAATGTGCA GACTAATATC
    TTCGGACCAA AGAAGAAGTT CAAACCTGTT GTCCAAAAAC CAATTCCGAA AGATACATCA
    CTGCACAGTG CCCTCATGGA AGCTATTCAA ACAGGAGGGG GAAAAGAGAA ACTCAGAAAG
    GTTTCAGACA GCGCGCTAAA TGGCAATCAC AAGAAACCAT CATACAGTGA GCCAGAGAAT
    GAGCGGTCGG CCCTACTAGC GGCAATTAGA GGCCACAGTG GCACTTGCAA GCTGAAAAAA
    ATCTCCTCAT TGGCCTCCGA AGAGCTCCAG AGTTTTAGGA ATGCAGAACT GTCTTCACAG
    AAAGCAGAAG ACCCTCAGGA AGAGCAGCTC TGCATTCCAC CTGCCCCTGC CCAGCCACCA
    CCACCGCCTC CCCCTCAGCT GTCAACAGCA ACTTTCAAAT TCTCTGCCAC AACTACGGGT
    GACCCTGGAG AGGCCAGGCG AGCCCTGATG GAGGCCATTC GCTCTGGTGC TGGAGCAGCA
    AAGTTAAAAA AGGTCCCTCT GCTCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137804.1
    CDS571..3960
    Translation

    Target ORF information:

    RefSeq Version XM_015282318.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282318.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAACAGAG ATTATGGTGA GGAGGATGTT TTTAGTTCTA CATCTACAAG TGAAGGAAGC 
    TTGGATGGTT TTTCCACAGC ACCAAACTCC CCTTCGGTGA ATTCCCGTTC CAGTACCTTG
    GGTCCATCGC TGTCCCTTGG TAATATCTCA GGAATGACAG CAAACCCAGA AGTGAAAAAG
    CGCAGAGCAC CTCCTCCACC AGTTTTGACA CCTCCGTTGC AGAATATGGA GATGAGCTCC
    GCTCAGGAGA AGACAGCAGT ACAGATTTCA CAAGGAGCAT CTCTTAACGA TCTACAGAAA
    AAGAAGAGGC GTGCACCTCT TCCACCAACT CAGTCTGCTC CACCTACTCC AGCCATGCCA
    AATAGGAGGG AAGAGATGGA AGACAAGAGG AAGAGCACAA TGGGTGATGG ACGGCAGGTG
    CCACAAAAGC CTCCTAGGGG CCCCACTCGT GACCCACCCC AGTTAGTGAT CCCCCCACCC
    CCACCTTACC CTCCTCCTGA AAGAGACAAT ATGGATCCAG CAGTCTCTTA CAGAGAAGCA
    GATGTTACAG CACCAACAAA ACTGGTACCT AAACAGAGCC TGCTGCCTGC ACATGATAAT
    TTTTATGTAG TGGATGATAC AGTTATGGAG CTATCAGAGG TAGAAGAAAC AGCGTCAGAA
    AGCAGCTGCT TTGCATCTGA GGACACTACC GAAGACTCAG GTGTAGTGAG CTCCCCATCT
    GACATTGTGT CACTGGATTC ACAAAATGAC AGTATGAAGT TGAGAGACAT CAAGCTGGTG
    AATGGCTATG TGGACTCAGA CACGATCTAT GGTGCAGAGA CTTGCTCTGT GAAGAACGCG
    TACTGCGATG ATATGGGATA TGCCGGTACT CCACAGAGCA CTAAAAATGA AGAAATGGCT
    GCGGCTAAGC ATGAGAATGA GGACATATTC ATTGATGCAC AACTCCAGCA GACTTTGGCT
    GACCTGGATG AAGATTTAGA AGCTATGGAT GTTTTAAGTA ACTCTGACTC TGAGCACATG
    AGTGAAGTGT TGAGCCCACA TTCAGGAAAA GTTGAGGTCC CCCAAGATGT TGCTAGTTCT
    GTTCCAGTAA CAGTCATAGA TGATGCTCCA GAAGTTACCA TCTATAACTC AGCTGGAAAC
    CAAGAGACAA GGGGTTCACA GAATCTATCA GCTGACTCTG TTAATATGGG AAACTTCACA
    AATAGAAACA ACAATGCAGG TTCCTTTGAT AAGGGACACA CTGATGGTGG AGCTGTATGT
    ATACCCAAGG ACCTAATACA TCAGCAGCCA TCTGATAATG AGTTCAGTCA CATACCCGTG
    GAACAGAGGA GAGATATGTT TGAATCTCTC ACGTCCTCCT TTGAAAAAAG AAGTGAAAAG
    AATGTAAGAG TAGAAGCTCC GCCCAGACAC GTTATGAAGT CTGAGGAGTG TAAATCGAAG
    CTGACGTCAT CTCACTCCAA GACTGCGACT GAAATCAATA CAGCAAAAGA GAAAGTAAAT
    CCATTTAATG AATGTAGTAA GAGGGAGAGA CCTCTGAAAA AAAGTAAGTC AGAACTAGAA
    ATCAAAAGGC CACCAGCAAA AAAGATTGAA GTAGAAGAAG TTCCATCAAC ACCTCCGTGG
    TGTCATCGTG CCCAGAAATT GGAAACAAGT TATGAACCCA AAATGGGTTT GACGACCTTC
    AAAGTTGTCC CTCCCAAACC TGAAGTAAAA AATTTTGATA GAGGGCTTTC AGTCTCCACT
    GGTGCTATTA AGATTGATGA ACTAGGCAAC CTTATCAACC CAAAGCCTGT TGTCAGTAAG
    AACATACCAG GTACCTCTGC TGGTGAAAGT GAGGAAATTC CTCTTGGGAA AGTGAAGGCA
    TTCTGGAGGT CAAGTTCTAT GGAAAAACAA AGTGATGACT CTGCTGAGCA TCTTGCTAAA
    AAGCCAGCAG TTACCACAAA CTCTAAGTCC TTTGTTACAA AGCAAGAATG CAAACCTGTC
    TGTCTTGCAG CTCCAAAACC TGTACCACCA CAAACTGTTA TTTCCCAGGT AGGTGAAAAG
    CAGTCTGAGA ATGAGAAGAC CAAACCACCT CTTCCAGTTA CACAGTCTCA GGTAAAACCA
    TTAGTGGTCC CAGCCATTAA TAAGGACAAG CCGGAGCTTC CATTTCTAAA GCCTCACAGA
    AGAACTTCCA GTCAGTATGT GGCCTCTGCC ATTGCAAAAC ACATCAATCT GCCTACCTTT
    AAGACTGATT CTACAGATTT GCATGAGAAA GATGAAAATA AGCACGGGGC AGGAGAGGTT
    AAAACTGAAG CTGAAGTTTC CCCCAAAAGG TGTATTATTG TGGCCAAGTA CAGCCCTGTG
    GAAACAGAAT CAGCAGAAAC AAGAGAAAAA ATTTCAAGTA TTTTTACTTG TAGTCAAAAA
    GCATCCCACA GATTCACACC TGGTGATAAT TCTGGCATGG AAAACAAAGT AGTGAACATC
    AGGGCACCGA GTCAAGCAAC TCCTGCTAGT TTCTATAAAA AGAATGCCAG TGTCCCACTT
    ACTAAATCCT CTTCAAAGGA TAACAACAAA GCAGAAGATG ATCACAGTTT AAACAGCAAA
    CAAGGTACAG CAGAGAAAAA GATGCATCTT TTGTTTGACC AGAAATACGA GACTTTGCCC
    CGTAGTAATT CAGCCTCCTC TCTCAAGTCA CCTGATCTCC AAGGAGCCTC ATCTTCTTTC
    AGTTCATCTT TGCGCATCAC TAAATGGCCT CCTGCTGACA GCGTGCAAGT TAGTTCCCTG
    AAAGCATCGC CCGAGTTCAG TGGTGTGACA GCAAAGGCAG AATCAGAACA GAATGACAAA
    TCTGATGATT CGGCTGCTAA TGCTATTGGC GAATTGGATG TTAATGTGCA GACTAATATC
    TTCGGACCAA AGAAGAAGTT CAAACCTGTT GTCCAAAAAC CAATTCCGAA AGATACATCA
    CTGCACAGTG CCCTCATGGA AGCTATTCAA ACAGGAGGGG GAAAAGAGAA ACTCAGAAAG
    GTTTCAGACA GCGCGCTAAA TGGCAATCAC AAGAAACCAT CATACAGTGA GCCAGAGAAT
    GAGCGGTCGG CCCTACTAGC GGCAATTAGA GGCCACAGTG GCACTTGCAA GCTGAAAAAA
    ATCTCCTCAT TGGCCTCCGA AGAGCTCCAG AGTTTTAGGA ATGCAGAACT GTCTTCACAG
    AAAGCAGAAG ACCCTCAGGA AGAGCAGCTC TGCATTCCAC CTGCCCCTGC CCAGCCACCA
    CCACCGCCTC CCCCTCAGCT GTCAACAGCA ACTTTCAAAT TCTCTGCCAC AACTACGGGT
    GACCCTGGAG AGGCCAGGCG AGCCCTGATG GAGGCCATTC GCTCTGGTGC TGGAGCAGCA
    AAGTTAAAAA AGGTCCCTCT GCTCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32272
    Clone ID Related Accession (Same CDS sequence) XM_015282318.1 , XM_015282318.2 , XM_015282319.1 , XM_015282319.2
    Accession Version XM_015282318.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3390bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein cordon-bleu isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015282318.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAACAGAG ATTATGGTGA GGAGGATGTT TTTAGTTCTA CATCTACAAG TGAAGGAAGC 
    TTGGATGGTT TTTCCACAGC ACCAAACTCC CCTTCGGTGA ATTCCCGTTC CAGTACCTTG
    GGTCCATCGC TGTCCCTTGG TAATATCTCA GGAATGACAG CAAACCCAGA AGTGAAAAAG
    CGCAGAGCAC CTCCTCCACC AGTTTTGACA CCTCCGTTGC AGAATATGGA GATGAGCTCC
    GCTCAGGAGA AGACAGCAGT ACAGATTTCA CAAGGAGCAT CTCTTAACGA TCTACAGAAA
    AAGAAGAGGC GTGCACCTCT TCCACCAACT CAGTCTGCTC CACCTACTCC AGCCATGCCA
    AATAGGAGGG AAGAGATGGA AGACAAGAGG AAGAGCACAA TGGGTGATGG ACGGCAGGTG
    CCACAAAAGC CTCCTAGGGG CCCCACTCGT GACCCACCCC AGTTAGTGAT CCCCCCACCC
    CCACCTTACC CTCCTCCTGA AAGAGACAAT ATGGATCCAG CAGTCTCTTA CAGAGAAGCA
    GATGTTACAG CACCAACAAA ACTGGTACCT AAACAGAGCC TGCTGCCTGC ACATGATAAT
    TTTTATGTAG TGGATGATAC AGTTATGGAG CTATCAGAGG TAGAAGAAAC AGCGTCAGAA
    AGCAGCTGCT TTGCATCTGA GGACACTACC GAAGACTCAG GTGTAGTGAG CTCCCCATCT
    GACATTGTGT CACTGGATTC ACAAAATGAC AGTATGAAGT TGAGAGACAT CAAGCTGGTG
    AATGGCTATG TGGACTCAGA CACGATCTAT GGTGCAGAGA CTTGCTCTGT GAAGAACGCG
    TACTGCGATG ATATGGGATA TGCCGGTACT CCACAGAGCA CTAAAAATGA AGAAATGGCT
    GCGGCTAAGC ATGAGAATGA GGACATATTC ATTGATGCAC AACTCCAGCA GACTTTGGCT
    GACCTGGATG AAGATTTAGA AGCTATGGAT GTTTTAAGTA ACTCTGACTC TGAGCACATG
    AGTGAAGTGT TGAGCCCACA TTCAGGAAAA GTTGAGGTCC CCCAAGATGT TGCTAGTTCT
    GTTCCAGTAA CAGTCATAGA TGATGCTCCA GAAGTTACCA TCTATAACTC AGCTGGAAAC
    CAAGAGACAA GGGGTTCACA GAATCTATCA GCTGACTCTG TTAATATGGG AAACTTCACA
    AATAGAAACA ACAATGCAGG TTCCTTTGAT AAGGGACACA CTGATGGTGG AGCTGTATGT
    ATACCCAAGG ACCTAATACA TCAGCAGCCA TCTGATAATG AGTTCAGTCA CATACCCGTG
    GAACAGAGGA GAGATATGTT TGAATCTCTC ACGTCCTCCT TTGAAAAAAG AAGTGAAAAG
    AATGTAAGAG TAGAAGCTCC GCCCAGACAC GTTATGAAGT CTGAGGAGTG TAAATCGAAG
    CTGACGTCAT CTCACTCCAA GACTGCGACT GAAATCAATA CAGCAAAAGA GAAAGTAAAT
    CCATTTAATG AATGTAGTAA GAGGGAGAGA CCTCTGAAAA AAAGTAAGTC AGAACTAGAA
    ATCAAAAGGC CACCAGCAAA AAAGATTGAA GTAGAAGAAG TTCCATCAAC ACCTCCGTGG
    TGTCATCGTG CCCAGAAATT GGAAACAAGT TATGAACCCA AAATGGGTTT GACGACCTTC
    AAAGTTGTCC CTCCCAAACC TGAAGTAAAA AATTTTGATA GAGGGCTTTC AGTCTCCACT
    GGTGCTATTA AGATTGATGA ACTAGGCAAC CTTATCAACC CAAAGCCTGT TGTCAGTAAG
    AACATACCAG GTACCTCTGC TGGTGAAAGT GAGGAAATTC CTCTTGGGAA AGTGAAGGCA
    TTCTGGAGGT CAAGTTCTAT GGAAAAACAA AGTGATGACT CTGCTGAGCA TCTTGCTAAA
    AAGCCAGCAG TTACCACAAA CTCTAAGTCC TTTGTTACAA AGCAAGAATG CAAACCTGTC
    TGTCTTGCAG CTCCAAAACC TGTACCACCA CAAACTGTTA TTTCCCAGGT AGGTGAAAAG
    CAGTCTGAGA ATGAGAAGAC CAAACCACCT CTTCCAGTTA CACAGTCTCA GGTAAAACCA
    TTAGTGGTCC CAGCCATTAA TAAGGACAAG CCGGAGCTTC CATTTCTAAA GCCTCACAGA
    AGAACTTCCA GTCAGTATGT GGCCTCTGCC ATTGCAAAAC ACATCAATCT GCCTACCTTT
    AAGACTGATT CTACAGATTT GCATGAGAAA GATGAAAATA AGCACGGGGC AGGAGAGGTT
    AAAACTGAAG CTGAAGTTTC CCCCAAAAGG TGTATTATTG TGGCCAAGTA CAGCCCTGTG
    GAAACAGAAT CAGCAGAAAC AAGAGAAAAA ATTTCAAGTA TTTTTACTTG TAGTCAAAAA
    GCATCCCACA GATTCACACC TGGTGATAAT TCTGGCATGG AAAACAAAGT AGTGAACATC
    AGGGCACCGA GTCAAGCAAC TCCTGCTAGT TTCTATAAAA AGAATGCCAG TGTCCCACTT
    ACTAAATCCT CTTCAAAGGA TAACAACAAA GCAGAAGATG ATCACAGTTT AAACAGCAAA
    CAAGGTACAG CAGAGAAAAA GATGCATCTT TTGTTTGACC AGAAATACGA GACTTTGCCC
    CGTAGTAATT CAGCCTCCTC TCTCAAGTCA CCTGATCTCC AAGGAGCCTC ATCTTCTTTC
    AGTTCATCTT TGCGCATCAC TAAATGGCCT CCTGCTGACA GCGTGCAAGT TAGTTCCCTG
    AAAGCATCGC CCGAGTTCAG TGGTGTGACA GCAAAGGCAG AATCAGAACA GAATGACAAA
    TCTGATGATT CGGCTGCTAA TGCTATTGGC GAATTGGATG TTAATGTGCA GACTAATATC
    TTCGGACCAA AGAAGAAGTT CAAACCTGTT GTCCAAAAAC CAATTCCGAA AGATACATCA
    CTGCACAGTG CCCTCATGGA AGCTATTCAA ACAGGAGGGG GAAAAGAGAA ACTCAGAAAG
    GTTTCAGACA GCGCGCTAAA TGGCAATCAC AAGAAACCAT CATACAGTGA GCCAGAGAAT
    GAGCGGTCGG CCCTACTAGC GGCAATTAGA GGCCACAGTG GCACTTGCAA GCTGAAAAAA
    ATCTCCTCAT TGGCCTCCGA AGAGCTCCAG AGTTTTAGGA ATGCAGAACT GTCTTCACAG
    AAAGCAGAAG ACCCTCAGGA AGAGCAGCTC TGCATTCCAC CTGCCCCTGC CCAGCCACCA
    CCACCGCCTC CCCCTCAGCT GTCAACAGCA ACTTTCAAAT TCTCTGCCAC AACTACGGGT
    GACCCTGGAG AGGCCAGGCG AGCCCTGATG GAGGCCATTC GCTCTGGTGC TGGAGCAGCA
    AAGTTAAAAA AGGTCCCTCT GCTCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137804.1
    CDS564..3953
    Translation

    Target ORF information:

    RefSeq Version XM_015282318.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282318.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAACAGAG ATTATGGTGA GGAGGATGTT TTTAGTTCTA CATCTACAAG TGAAGGAAGC 
    TTGGATGGTT TTTCCACAGC ACCAAACTCC CCTTCGGTGA ATTCCCGTTC CAGTACCTTG
    GGTCCATCGC TGTCCCTTGG TAATATCTCA GGAATGACAG CAAACCCAGA AGTGAAAAAG
    CGCAGAGCAC CTCCTCCACC AGTTTTGACA CCTCCGTTGC AGAATATGGA GATGAGCTCC
    GCTCAGGAGA AGACAGCAGT ACAGATTTCA CAAGGAGCAT CTCTTAACGA TCTACAGAAA
    AAGAAGAGGC GTGCACCTCT TCCACCAACT CAGTCTGCTC CACCTACTCC AGCCATGCCA
    AATAGGAGGG AAGAGATGGA AGACAAGAGG AAGAGCACAA TGGGTGATGG ACGGCAGGTG
    CCACAAAAGC CTCCTAGGGG CCCCACTCGT GACCCACCCC AGTTAGTGAT CCCCCCACCC
    CCACCTTACC CTCCTCCTGA AAGAGACAAT ATGGATCCAG CAGTCTCTTA CAGAGAAGCA
    GATGTTACAG CACCAACAAA ACTGGTACCT AAACAGAGCC TGCTGCCTGC ACATGATAAT
    TTTTATGTAG TGGATGATAC AGTTATGGAG CTATCAGAGG TAGAAGAAAC AGCGTCAGAA
    AGCAGCTGCT TTGCATCTGA GGACACTACC GAAGACTCAG GTGTAGTGAG CTCCCCATCT
    GACATTGTGT CACTGGATTC ACAAAATGAC AGTATGAAGT TGAGAGACAT CAAGCTGGTG
    AATGGCTATG TGGACTCAGA CACGATCTAT GGTGCAGAGA CTTGCTCTGT GAAGAACGCG
    TACTGCGATG ATATGGGATA TGCCGGTACT CCACAGAGCA CTAAAAATGA AGAAATGGCT
    GCGGCTAAGC ATGAGAATGA GGACATATTC ATTGATGCAC AACTCCAGCA GACTTTGGCT
    GACCTGGATG AAGATTTAGA AGCTATGGAT GTTTTAAGTA ACTCTGACTC TGAGCACATG
    AGTGAAGTGT TGAGCCCACA TTCAGGAAAA GTTGAGGTCC CCCAAGATGT TGCTAGTTCT
    GTTCCAGTAA CAGTCATAGA TGATGCTCCA GAAGTTACCA TCTATAACTC AGCTGGAAAC
    CAAGAGACAA GGGGTTCACA GAATCTATCA GCTGACTCTG TTAATATGGG AAACTTCACA
    AATAGAAACA ACAATGCAGG TTCCTTTGAT AAGGGACACA CTGATGGTGG AGCTGTATGT
    ATACCCAAGG ACCTAATACA TCAGCAGCCA TCTGATAATG AGTTCAGTCA CATACCCGTG
    GAACAGAGGA GAGATATGTT TGAATCTCTC ACGTCCTCCT TTGAAAAAAG AAGTGAAAAG
    AATGTAAGAG TAGAAGCTCC GCCCAGACAC GTTATGAAGT CTGAGGAGTG TAAATCGAAG
    CTGACGTCAT CTCACTCCAA GACTGCGACT GAAATCAATA CAGCAAAAGA GAAAGTAAAT
    CCATTTAATG AATGTAGTAA GAGGGAGAGA CCTCTGAAAA AAAGTAAGTC AGAACTAGAA
    ATCAAAAGGC CACCAGCAAA AAAGATTGAA GTAGAAGAAG TTCCATCAAC ACCTCCGTGG
    TGTCATCGTG CCCAGAAATT GGAAACAAGT TATGAACCCA AAATGGGTTT GACGACCTTC
    AAAGTTGTCC CTCCCAAACC TGAAGTAAAA AATTTTGATA GAGGGCTTTC AGTCTCCACT
    GGTGCTATTA AGATTGATGA ACTAGGCAAC CTTATCAACC CAAAGCCTGT TGTCAGTAAG
    AACATACCAG GTACCTCTGC TGGTGAAAGT GAGGAAATTC CTCTTGGGAA AGTGAAGGCA
    TTCTGGAGGT CAAGTTCTAT GGAAAAACAA AGTGATGACT CTGCTGAGCA TCTTGCTAAA
    AAGCCAGCAG TTACCACAAA CTCTAAGTCC TTTGTTACAA AGCAAGAATG CAAACCTGTC
    TGTCTTGCAG CTCCAAAACC TGTACCACCA CAAACTGTTA TTTCCCAGGT AGGTGAAAAG
    CAGTCTGAGA ATGAGAAGAC CAAACCACCT CTTCCAGTTA CACAGTCTCA GGTAAAACCA
    TTAGTGGTCC CAGCCATTAA TAAGGACAAG CCGGAGCTTC CATTTCTAAA GCCTCACAGA
    AGAACTTCCA GTCAGTATGT GGCCTCTGCC ATTGCAAAAC ACATCAATCT GCCTACCTTT
    AAGACTGATT CTACAGATTT GCATGAGAAA GATGAAAATA AGCACGGGGC AGGAGAGGTT
    AAAACTGAAG CTGAAGTTTC CCCCAAAAGG TGTATTATTG TGGCCAAGTA CAGCCCTGTG
    GAAACAGAAT CAGCAGAAAC AAGAGAAAAA ATTTCAAGTA TTTTTACTTG TAGTCAAAAA
    GCATCCCACA GATTCACACC TGGTGATAAT TCTGGCATGG AAAACAAAGT AGTGAACATC
    AGGGCACCGA GTCAAGCAAC TCCTGCTAGT TTCTATAAAA AGAATGCCAG TGTCCCACTT
    ACTAAATCCT CTTCAAAGGA TAACAACAAA GCAGAAGATG ATCACAGTTT AAACAGCAAA
    CAAGGTACAG CAGAGAAAAA GATGCATCTT TTGTTTGACC AGAAATACGA GACTTTGCCC
    CGTAGTAATT CAGCCTCCTC TCTCAAGTCA CCTGATCTCC AAGGAGCCTC ATCTTCTTTC
    AGTTCATCTT TGCGCATCAC TAAATGGCCT CCTGCTGACA GCGTGCAAGT TAGTTCCCTG
    AAAGCATCGC CCGAGTTCAG TGGTGTGACA GCAAAGGCAG AATCAGAACA GAATGACAAA
    TCTGATGATT CGGCTGCTAA TGCTATTGGC GAATTGGATG TTAATGTGCA GACTAATATC
    TTCGGACCAA AGAAGAAGTT CAAACCTGTT GTCCAAAAAC CAATTCCGAA AGATACATCA
    CTGCACAGTG CCCTCATGGA AGCTATTCAA ACAGGAGGGG GAAAAGAGAA ACTCAGAAAG
    GTTTCAGACA GCGCGCTAAA TGGCAATCAC AAGAAACCAT CATACAGTGA GCCAGAGAAT
    GAGCGGTCGG CCCTACTAGC GGCAATTAGA GGCCACAGTG GCACTTGCAA GCTGAAAAAA
    ATCTCCTCAT TGGCCTCCGA AGAGCTCCAG AGTTTTAGGA ATGCAGAACT GTCTTCACAG
    AAAGCAGAAG ACCCTCAGGA AGAGCAGCTC TGCATTCCAC CTGCCCCTGC CCAGCCACCA
    CCACCGCCTC CCCCTCAGCT GTCAACAGCA ACTTTCAAAT TCTCTGCCAC AACTACGGGT
    GACCCTGGAG AGGCCAGGCG AGCCCTGATG GAGGCCATTC GCTCTGGTGC TGGAGCAGCA
    AAGTTAAAAA AGGTCCCTCT GCTCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32272
    Clone ID Related Accession (Same CDS sequence) XM_015282318.1 , XM_015282318.2 , XM_015282319.1 , XM_015282319.2
    Accession Version XM_015282319.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3390bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product protein cordon-bleu isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455789.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAACAGAG ATTATGGTGA GGAGGATGTT TTTAGTTCTA CATCTACAAG TGAAGGAAGC 
    TTGGATGGTT TTTCCACAGC ACCAAACTCC CCTTCGGTGA ATTCCCGTTC CAGTACCTTG
    GGTCCATCGC TGTCCCTTGG TAATATCTCA GGAATGACAG CAAACCCAGA AGTGAAAAAG
    CGCAGAGCAC CTCCTCCACC AGTTTTGACA CCTCCGTTGC AGAATATGGA GATGAGCTCC
    GCTCAGGAGA AGACAGCAGT ACAGATTTCA CAAGGAGCAT CTCTTAACGA TCTACAGAAA
    AAGAAGAGGC GTGCACCTCT TCCACCAACT CAGTCTGCTC CACCTACTCC AGCCATGCCA
    AATAGGAGGG AAGAGATGGA AGACAAGAGG AAGAGCACAA TGGGTGATGG ACGGCAGGTG
    CCACAAAAGC CTCCTAGGGG CCCCACTCGT GACCCACCCC AGTTAGTGAT CCCCCCACCC
    CCACCTTACC CTCCTCCTGA AAGAGACAAT ATGGATCCAG CAGTCTCTTA CAGAGAAGCA
    GATGTTACAG CACCAACAAA ACTGGTACCT AAACAGAGCC TGCTGCCTGC ACATGATAAT
    TTTTATGTAG TGGATGATAC AGTTATGGAG CTATCAGAGG TAGAAGAAAC AGCGTCAGAA
    AGCAGCTGCT TTGCATCTGA GGACACTACC GAAGACTCAG GTGTAGTGAG CTCCCCATCT
    GACATTGTGT CACTGGATTC ACAAAATGAC AGTATGAAGT TGAGAGACAT CAAGCTGGTG
    AATGGCTATG TGGACTCAGA CACGATCTAT GGTGCAGAGA CTTGCTCTGT GAAGAACGCG
    TACTGCGATG ATATGGGATA TGCCGGTACT CCACAGAGCA CTAAAAATGA AGAAATGGCT
    GCGGCTAAGC ATGAGAATGA GGACATATTC ATTGATGCAC AACTCCAGCA GACTTTGGCT
    GACCTGGATG AAGATTTAGA AGCTATGGAT GTTTTAAGTA ACTCTGACTC TGAGCACATG
    AGTGAAGTGT TGAGCCCACA TTCAGGAAAA GTTGAGGTCC CCCAAGATGT TGCTAGTTCT
    GTTCCAGTAA CAGTCATAGA TGATGCTCCA GAAGTTACCA TCTATAACTC AGCTGGAAAC
    CAAGAGACAA GGGGTTCACA GAATCTATCA GCTGACTCTG TTAATATGGG AAACTTCACA
    AATAGAAACA ACAATGCAGG TTCCTTTGAT AAGGGACACA CTGATGGTGG AGCTGTATGT
    ATACCCAAGG ACCTAATACA TCAGCAGCCA TCTGATAATG AGTTCAGTCA CATACCCGTG
    GAACAGAGGA GAGATATGTT TGAATCTCTC ACGTCCTCCT TTGAAAAAAG AAGTGAAAAG
    AATGTAAGAG TAGAAGCTCC GCCCAGACAC GTTATGAAGT CTGAGGAGTG TAAATCGAAG
    CTGACGTCAT CTCACTCCAA GACTGCGACT GAAATCAATA CAGCAAAAGA GAAAGTAAAT
    CCATTTAATG AATGTAGTAA GAGGGAGAGA CCTCTGAAAA AAAGTAAGTC AGAACTAGAA
    ATCAAAAGGC CACCAGCAAA AAAGATTGAA GTAGAAGAAG TTCCATCAAC ACCTCCGTGG
    TGTCATCGTG CCCAGAAATT GGAAACAAGT TATGAACCCA AAATGGGTTT GACGACCTTC
    AAAGTTGTCC CTCCCAAACC TGAAGTAAAA AATTTTGATA GAGGGCTTTC AGTCTCCACT
    GGTGCTATTA AGATTGATGA ACTAGGCAAC CTTATCAACC CAAAGCCTGT TGTCAGTAAG
    AACATACCAG GTACCTCTGC TGGTGAAAGT GAGGAAATTC CTCTTGGGAA AGTGAAGGCA
    TTCTGGAGGT CAAGTTCTAT GGAAAAACAA AGTGATGACT CTGCTGAGCA TCTTGCTAAA
    AAGCCAGCAG TTACCACAAA CTCTAAGTCC TTTGTTACAA AGCAAGAATG CAAACCTGTC
    TGTCTTGCAG CTCCAAAACC TGTACCACCA CAAACTGTTA TTTCCCAGGT AGGTGAAAAG
    CAGTCTGAGA ATGAGAAGAC CAAACCACCT CTTCCAGTTA CACAGTCTCA GGTAAAACCA
    TTAGTGGTCC CAGCCATTAA TAAGGACAAG CCGGAGCTTC CATTTCTAAA GCCTCACAGA
    AGAACTTCCA GTCAGTATGT GGCCTCTGCC ATTGCAAAAC ACATCAATCT GCCTACCTTT
    AAGACTGATT CTACAGATTT GCATGAGAAA GATGAAAATA AGCACGGGGC AGGAGAGGTT
    AAAACTGAAG CTGAAGTTTC CCCCAAAAGG TGTATTATTG TGGCCAAGTA CAGCCCTGTG
    GAAACAGAAT CAGCAGAAAC AAGAGAAAAA ATTTCAAGTA TTTTTACTTG TAGTCAAAAA
    GCATCCCACA GATTCACACC TGGTGATAAT TCTGGCATGG AAAACAAAGT AGTGAACATC
    AGGGCACCGA GTCAAGCAAC TCCTGCTAGT TTCTATAAAA AGAATGCCAG TGTCCCACTT
    ACTAAATCCT CTTCAAAGGA TAACAACAAA GCAGAAGATG ATCACAGTTT AAACAGCAAA
    CAAGGTACAG CAGAGAAAAA GATGCATCTT TTGTTTGACC AGAAATACGA GACTTTGCCC
    CGTAGTAATT CAGCCTCCTC TCTCAAGTCA CCTGATCTCC AAGGAGCCTC ATCTTCTTTC
    AGTTCATCTT TGCGCATCAC TAAATGGCCT CCTGCTGACA GCGTGCAAGT TAGTTCCCTG
    AAAGCATCGC CCGAGTTCAG TGGTGTGACA GCAAAGGCAG AATCAGAACA GAATGACAAA
    TCTGATGATT CGGCTGCTAA TGCTATTGGC GAATTGGATG TTAATGTGCA GACTAATATC
    TTCGGACCAA AGAAGAAGTT CAAACCTGTT GTCCAAAAAC CAATTCCGAA AGATACATCA
    CTGCACAGTG CCCTCATGGA AGCTATTCAA ACAGGAGGGG GAAAAGAGAA ACTCAGAAAG
    GTTTCAGACA GCGCGCTAAA TGGCAATCAC AAGAAACCAT CATACAGTGA GCCAGAGAAT
    GAGCGGTCGG CCCTACTAGC GGCAATTAGA GGCCACAGTG GCACTTGCAA GCTGAAAAAA
    ATCTCCTCAT TGGCCTCCGA AGAGCTCCAG AGTTTTAGGA ATGCAGAACT GTCTTCACAG
    AAAGCAGAAG ACCCTCAGGA AGAGCAGCTC TGCATTCCAC CTGCCCCTGC CCAGCCACCA
    CCACCGCCTC CCCCTCAGCT GTCAACAGCA ACTTTCAAAT TCTCTGCCAC AACTACGGGT
    GACCCTGGAG AGGCCAGGCG AGCCCTGATG GAGGCCATTC GCTCTGGTGC TGGAGCAGCA
    AAGTTAAAAA AGGTCCCTCT GCTCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137805.1
    CDS526..3915
    Translation

    Target ORF information:

    RefSeq Version XM_015282319.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282319.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAACAGAG ATTATGGTGA GGAGGATGTT TTTAGTTCTA CATCTACAAG TGAAGGAAGC 
    TTGGATGGTT TTTCCACAGC ACCAAACTCC CCTTCGGTGA ATTCCCGTTC CAGTACCTTG
    GGTCCATCGC TGTCCCTTGG TAATATCTCA GGAATGACAG CAAACCCAGA AGTGAAAAAG
    CGCAGAGCAC CTCCTCCACC AGTTTTGACA CCTCCGTTGC AGAATATGGA GATGAGCTCC
    GCTCAGGAGA AGACAGCAGT ACAGATTTCA CAAGGAGCAT CTCTTAACGA TCTACAGAAA
    AAGAAGAGGC GTGCACCTCT TCCACCAACT CAGTCTGCTC CACCTACTCC AGCCATGCCA
    AATAGGAGGG AAGAGATGGA AGACAAGAGG AAGAGCACAA TGGGTGATGG ACGGCAGGTG
    CCACAAAAGC CTCCTAGGGG CCCCACTCGT GACCCACCCC AGTTAGTGAT CCCCCCACCC
    CCACCTTACC CTCCTCCTGA AAGAGACAAT ATGGATCCAG CAGTCTCTTA CAGAGAAGCA
    GATGTTACAG CACCAACAAA ACTGGTACCT AAACAGAGCC TGCTGCCTGC ACATGATAAT
    TTTTATGTAG TGGATGATAC AGTTATGGAG CTATCAGAGG TAGAAGAAAC AGCGTCAGAA
    AGCAGCTGCT TTGCATCTGA GGACACTACC GAAGACTCAG GTGTAGTGAG CTCCCCATCT
    GACATTGTGT CACTGGATTC ACAAAATGAC AGTATGAAGT TGAGAGACAT CAAGCTGGTG
    AATGGCTATG TGGACTCAGA CACGATCTAT GGTGCAGAGA CTTGCTCTGT GAAGAACGCG
    TACTGCGATG ATATGGGATA TGCCGGTACT CCACAGAGCA CTAAAAATGA AGAAATGGCT
    GCGGCTAAGC ATGAGAATGA GGACATATTC ATTGATGCAC AACTCCAGCA GACTTTGGCT
    GACCTGGATG AAGATTTAGA AGCTATGGAT GTTTTAAGTA ACTCTGACTC TGAGCACATG
    AGTGAAGTGT TGAGCCCACA TTCAGGAAAA GTTGAGGTCC CCCAAGATGT TGCTAGTTCT
    GTTCCAGTAA CAGTCATAGA TGATGCTCCA GAAGTTACCA TCTATAACTC AGCTGGAAAC
    CAAGAGACAA GGGGTTCACA GAATCTATCA GCTGACTCTG TTAATATGGG AAACTTCACA
    AATAGAAACA ACAATGCAGG TTCCTTTGAT AAGGGACACA CTGATGGTGG AGCTGTATGT
    ATACCCAAGG ACCTAATACA TCAGCAGCCA TCTGATAATG AGTTCAGTCA CATACCCGTG
    GAACAGAGGA GAGATATGTT TGAATCTCTC ACGTCCTCCT TTGAAAAAAG AAGTGAAAAG
    AATGTAAGAG TAGAAGCTCC GCCCAGACAC GTTATGAAGT CTGAGGAGTG TAAATCGAAG
    CTGACGTCAT CTCACTCCAA GACTGCGACT GAAATCAATA CAGCAAAAGA GAAAGTAAAT
    CCATTTAATG AATGTAGTAA GAGGGAGAGA CCTCTGAAAA AAAGTAAGTC AGAACTAGAA
    ATCAAAAGGC CACCAGCAAA AAAGATTGAA GTAGAAGAAG TTCCATCAAC ACCTCCGTGG
    TGTCATCGTG CCCAGAAATT GGAAACAAGT TATGAACCCA AAATGGGTTT GACGACCTTC
    AAAGTTGTCC CTCCCAAACC TGAAGTAAAA AATTTTGATA GAGGGCTTTC AGTCTCCACT
    GGTGCTATTA AGATTGATGA ACTAGGCAAC CTTATCAACC CAAAGCCTGT TGTCAGTAAG
    AACATACCAG GTACCTCTGC TGGTGAAAGT GAGGAAATTC CTCTTGGGAA AGTGAAGGCA
    TTCTGGAGGT CAAGTTCTAT GGAAAAACAA AGTGATGACT CTGCTGAGCA TCTTGCTAAA
    AAGCCAGCAG TTACCACAAA CTCTAAGTCC TTTGTTACAA AGCAAGAATG CAAACCTGTC
    TGTCTTGCAG CTCCAAAACC TGTACCACCA CAAACTGTTA TTTCCCAGGT AGGTGAAAAG
    CAGTCTGAGA ATGAGAAGAC CAAACCACCT CTTCCAGTTA CACAGTCTCA GGTAAAACCA
    TTAGTGGTCC CAGCCATTAA TAAGGACAAG CCGGAGCTTC CATTTCTAAA GCCTCACAGA
    AGAACTTCCA GTCAGTATGT GGCCTCTGCC ATTGCAAAAC ACATCAATCT GCCTACCTTT
    AAGACTGATT CTACAGATTT GCATGAGAAA GATGAAAATA AGCACGGGGC AGGAGAGGTT
    AAAACTGAAG CTGAAGTTTC CCCCAAAAGG TGTATTATTG TGGCCAAGTA CAGCCCTGTG
    GAAACAGAAT CAGCAGAAAC AAGAGAAAAA ATTTCAAGTA TTTTTACTTG TAGTCAAAAA
    GCATCCCACA GATTCACACC TGGTGATAAT TCTGGCATGG AAAACAAAGT AGTGAACATC
    AGGGCACCGA GTCAAGCAAC TCCTGCTAGT TTCTATAAAA AGAATGCCAG TGTCCCACTT
    ACTAAATCCT CTTCAAAGGA TAACAACAAA GCAGAAGATG ATCACAGTTT AAACAGCAAA
    CAAGGTACAG CAGAGAAAAA GATGCATCTT TTGTTTGACC AGAAATACGA GACTTTGCCC
    CGTAGTAATT CAGCCTCCTC TCTCAAGTCA CCTGATCTCC AAGGAGCCTC ATCTTCTTTC
    AGTTCATCTT TGCGCATCAC TAAATGGCCT CCTGCTGACA GCGTGCAAGT TAGTTCCCTG
    AAAGCATCGC CCGAGTTCAG TGGTGTGACA GCAAAGGCAG AATCAGAACA GAATGACAAA
    TCTGATGATT CGGCTGCTAA TGCTATTGGC GAATTGGATG TTAATGTGCA GACTAATATC
    TTCGGACCAA AGAAGAAGTT CAAACCTGTT GTCCAAAAAC CAATTCCGAA AGATACATCA
    CTGCACAGTG CCCTCATGGA AGCTATTCAA ACAGGAGGGG GAAAAGAGAA ACTCAGAAAG
    GTTTCAGACA GCGCGCTAAA TGGCAATCAC AAGAAACCAT CATACAGTGA GCCAGAGAAT
    GAGCGGTCGG CCCTACTAGC GGCAATTAGA GGCCACAGTG GCACTTGCAA GCTGAAAAAA
    ATCTCCTCAT TGGCCTCCGA AGAGCTCCAG AGTTTTAGGA ATGCAGAACT GTCTTCACAG
    AAAGCAGAAG ACCCTCAGGA AGAGCAGCTC TGCATTCCAC CTGCCCCTGC CCAGCCACCA
    CCACCGCCTC CCCCTCAGCT GTCAACAGCA ACTTTCAAAT TCTCTGCCAC AACTACGGGT
    GACCCTGGAG AGGCCAGGCG AGCCCTGATG GAGGCCATTC GCTCTGGTGC TGGAGCAGCA
    AAGTTAAAAA AGGTCCCTCT GCTCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32272
    Clone ID Related Accession (Same CDS sequence) XM_015282318.1 , XM_015282318.2 , XM_015282319.1 , XM_015282319.2
    Accession Version XM_015282319.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3390bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein cordon-bleu isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015282319.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAACAGAG ATTATGGTGA GGAGGATGTT TTTAGTTCTA CATCTACAAG TGAAGGAAGC 
    TTGGATGGTT TTTCCACAGC ACCAAACTCC CCTTCGGTGA ATTCCCGTTC CAGTACCTTG
    GGTCCATCGC TGTCCCTTGG TAATATCTCA GGAATGACAG CAAACCCAGA AGTGAAAAAG
    CGCAGAGCAC CTCCTCCACC AGTTTTGACA CCTCCGTTGC AGAATATGGA GATGAGCTCC
    GCTCAGGAGA AGACAGCAGT ACAGATTTCA CAAGGAGCAT CTCTTAACGA TCTACAGAAA
    AAGAAGAGGC GTGCACCTCT TCCACCAACT CAGTCTGCTC CACCTACTCC AGCCATGCCA
    AATAGGAGGG AAGAGATGGA AGACAAGAGG AAGAGCACAA TGGGTGATGG ACGGCAGGTG
    CCACAAAAGC CTCCTAGGGG CCCCACTCGT GACCCACCCC AGTTAGTGAT CCCCCCACCC
    CCACCTTACC CTCCTCCTGA AAGAGACAAT ATGGATCCAG CAGTCTCTTA CAGAGAAGCA
    GATGTTACAG CACCAACAAA ACTGGTACCT AAACAGAGCC TGCTGCCTGC ACATGATAAT
    TTTTATGTAG TGGATGATAC AGTTATGGAG CTATCAGAGG TAGAAGAAAC AGCGTCAGAA
    AGCAGCTGCT TTGCATCTGA GGACACTACC GAAGACTCAG GTGTAGTGAG CTCCCCATCT
    GACATTGTGT CACTGGATTC ACAAAATGAC AGTATGAAGT TGAGAGACAT CAAGCTGGTG
    AATGGCTATG TGGACTCAGA CACGATCTAT GGTGCAGAGA CTTGCTCTGT GAAGAACGCG
    TACTGCGATG ATATGGGATA TGCCGGTACT CCACAGAGCA CTAAAAATGA AGAAATGGCT
    GCGGCTAAGC ATGAGAATGA GGACATATTC ATTGATGCAC AACTCCAGCA GACTTTGGCT
    GACCTGGATG AAGATTTAGA AGCTATGGAT GTTTTAAGTA ACTCTGACTC TGAGCACATG
    AGTGAAGTGT TGAGCCCACA TTCAGGAAAA GTTGAGGTCC CCCAAGATGT TGCTAGTTCT
    GTTCCAGTAA CAGTCATAGA TGATGCTCCA GAAGTTACCA TCTATAACTC AGCTGGAAAC
    CAAGAGACAA GGGGTTCACA GAATCTATCA GCTGACTCTG TTAATATGGG AAACTTCACA
    AATAGAAACA ACAATGCAGG TTCCTTTGAT AAGGGACACA CTGATGGTGG AGCTGTATGT
    ATACCCAAGG ACCTAATACA TCAGCAGCCA TCTGATAATG AGTTCAGTCA CATACCCGTG
    GAACAGAGGA GAGATATGTT TGAATCTCTC ACGTCCTCCT TTGAAAAAAG AAGTGAAAAG
    AATGTAAGAG TAGAAGCTCC GCCCAGACAC GTTATGAAGT CTGAGGAGTG TAAATCGAAG
    CTGACGTCAT CTCACTCCAA GACTGCGACT GAAATCAATA CAGCAAAAGA GAAAGTAAAT
    CCATTTAATG AATGTAGTAA GAGGGAGAGA CCTCTGAAAA AAAGTAAGTC AGAACTAGAA
    ATCAAAAGGC CACCAGCAAA AAAGATTGAA GTAGAAGAAG TTCCATCAAC ACCTCCGTGG
    TGTCATCGTG CCCAGAAATT GGAAACAAGT TATGAACCCA AAATGGGTTT GACGACCTTC
    AAAGTTGTCC CTCCCAAACC TGAAGTAAAA AATTTTGATA GAGGGCTTTC AGTCTCCACT
    GGTGCTATTA AGATTGATGA ACTAGGCAAC CTTATCAACC CAAAGCCTGT TGTCAGTAAG
    AACATACCAG GTACCTCTGC TGGTGAAAGT GAGGAAATTC CTCTTGGGAA AGTGAAGGCA
    TTCTGGAGGT CAAGTTCTAT GGAAAAACAA AGTGATGACT CTGCTGAGCA TCTTGCTAAA
    AAGCCAGCAG TTACCACAAA CTCTAAGTCC TTTGTTACAA AGCAAGAATG CAAACCTGTC
    TGTCTTGCAG CTCCAAAACC TGTACCACCA CAAACTGTTA TTTCCCAGGT AGGTGAAAAG
    CAGTCTGAGA ATGAGAAGAC CAAACCACCT CTTCCAGTTA CACAGTCTCA GGTAAAACCA
    TTAGTGGTCC CAGCCATTAA TAAGGACAAG CCGGAGCTTC CATTTCTAAA GCCTCACAGA
    AGAACTTCCA GTCAGTATGT GGCCTCTGCC ATTGCAAAAC ACATCAATCT GCCTACCTTT
    AAGACTGATT CTACAGATTT GCATGAGAAA GATGAAAATA AGCACGGGGC AGGAGAGGTT
    AAAACTGAAG CTGAAGTTTC CCCCAAAAGG TGTATTATTG TGGCCAAGTA CAGCCCTGTG
    GAAACAGAAT CAGCAGAAAC AAGAGAAAAA ATTTCAAGTA TTTTTACTTG TAGTCAAAAA
    GCATCCCACA GATTCACACC TGGTGATAAT TCTGGCATGG AAAACAAAGT AGTGAACATC
    AGGGCACCGA GTCAAGCAAC TCCTGCTAGT TTCTATAAAA AGAATGCCAG TGTCCCACTT
    ACTAAATCCT CTTCAAAGGA TAACAACAAA GCAGAAGATG ATCACAGTTT AAACAGCAAA
    CAAGGTACAG CAGAGAAAAA GATGCATCTT TTGTTTGACC AGAAATACGA GACTTTGCCC
    CGTAGTAATT CAGCCTCCTC TCTCAAGTCA CCTGATCTCC AAGGAGCCTC ATCTTCTTTC
    AGTTCATCTT TGCGCATCAC TAAATGGCCT CCTGCTGACA GCGTGCAAGT TAGTTCCCTG
    AAAGCATCGC CCGAGTTCAG TGGTGTGACA GCAAAGGCAG AATCAGAACA GAATGACAAA
    TCTGATGATT CGGCTGCTAA TGCTATTGGC GAATTGGATG TTAATGTGCA GACTAATATC
    TTCGGACCAA AGAAGAAGTT CAAACCTGTT GTCCAAAAAC CAATTCCGAA AGATACATCA
    CTGCACAGTG CCCTCATGGA AGCTATTCAA ACAGGAGGGG GAAAAGAGAA ACTCAGAAAG
    GTTTCAGACA GCGCGCTAAA TGGCAATCAC AAGAAACCAT CATACAGTGA GCCAGAGAAT
    GAGCGGTCGG CCCTACTAGC GGCAATTAGA GGCCACAGTG GCACTTGCAA GCTGAAAAAA
    ATCTCCTCAT TGGCCTCCGA AGAGCTCCAG AGTTTTAGGA ATGCAGAACT GTCTTCACAG
    AAAGCAGAAG ACCCTCAGGA AGAGCAGCTC TGCATTCCAC CTGCCCCTGC CCAGCCACCA
    CCACCGCCTC CCCCTCAGCT GTCAACAGCA ACTTTCAAAT TCTCTGCCAC AACTACGGGT
    GACCCTGGAG AGGCCAGGCG AGCCCTGATG GAGGCCATTC GCTCTGGTGC TGGAGCAGCA
    AAGTTAAAAA AGGTCCCTCT GCTCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137805.1
    CDS519..3908
    Translation

    Target ORF information:

    RefSeq Version XM_015282319.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282319.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAACAGAG ATTATGGTGA GGAGGATGTT TTTAGTTCTA CATCTACAAG TGAAGGAAGC 
    TTGGATGGTT TTTCCACAGC ACCAAACTCC CCTTCGGTGA ATTCCCGTTC CAGTACCTTG
    GGTCCATCGC TGTCCCTTGG TAATATCTCA GGAATGACAG CAAACCCAGA AGTGAAAAAG
    CGCAGAGCAC CTCCTCCACC AGTTTTGACA CCTCCGTTGC AGAATATGGA GATGAGCTCC
    GCTCAGGAGA AGACAGCAGT ACAGATTTCA CAAGGAGCAT CTCTTAACGA TCTACAGAAA
    AAGAAGAGGC GTGCACCTCT TCCACCAACT CAGTCTGCTC CACCTACTCC AGCCATGCCA
    AATAGGAGGG AAGAGATGGA AGACAAGAGG AAGAGCACAA TGGGTGATGG ACGGCAGGTG
    CCACAAAAGC CTCCTAGGGG CCCCACTCGT GACCCACCCC AGTTAGTGAT CCCCCCACCC
    CCACCTTACC CTCCTCCTGA AAGAGACAAT ATGGATCCAG CAGTCTCTTA CAGAGAAGCA
    GATGTTACAG CACCAACAAA ACTGGTACCT AAACAGAGCC TGCTGCCTGC ACATGATAAT
    TTTTATGTAG TGGATGATAC AGTTATGGAG CTATCAGAGG TAGAAGAAAC AGCGTCAGAA
    AGCAGCTGCT TTGCATCTGA GGACACTACC GAAGACTCAG GTGTAGTGAG CTCCCCATCT
    GACATTGTGT CACTGGATTC ACAAAATGAC AGTATGAAGT TGAGAGACAT CAAGCTGGTG
    AATGGCTATG TGGACTCAGA CACGATCTAT GGTGCAGAGA CTTGCTCTGT GAAGAACGCG
    TACTGCGATG ATATGGGATA TGCCGGTACT CCACAGAGCA CTAAAAATGA AGAAATGGCT
    GCGGCTAAGC ATGAGAATGA GGACATATTC ATTGATGCAC AACTCCAGCA GACTTTGGCT
    GACCTGGATG AAGATTTAGA AGCTATGGAT GTTTTAAGTA ACTCTGACTC TGAGCACATG
    AGTGAAGTGT TGAGCCCACA TTCAGGAAAA GTTGAGGTCC CCCAAGATGT TGCTAGTTCT
    GTTCCAGTAA CAGTCATAGA TGATGCTCCA GAAGTTACCA TCTATAACTC AGCTGGAAAC
    CAAGAGACAA GGGGTTCACA GAATCTATCA GCTGACTCTG TTAATATGGG AAACTTCACA
    AATAGAAACA ACAATGCAGG TTCCTTTGAT AAGGGACACA CTGATGGTGG AGCTGTATGT
    ATACCCAAGG ACCTAATACA TCAGCAGCCA TCTGATAATG AGTTCAGTCA CATACCCGTG
    GAACAGAGGA GAGATATGTT TGAATCTCTC ACGTCCTCCT TTGAAAAAAG AAGTGAAAAG
    AATGTAAGAG TAGAAGCTCC GCCCAGACAC GTTATGAAGT CTGAGGAGTG TAAATCGAAG
    CTGACGTCAT CTCACTCCAA GACTGCGACT GAAATCAATA CAGCAAAAGA GAAAGTAAAT
    CCATTTAATG AATGTAGTAA GAGGGAGAGA CCTCTGAAAA AAAGTAAGTC AGAACTAGAA
    ATCAAAAGGC CACCAGCAAA AAAGATTGAA GTAGAAGAAG TTCCATCAAC ACCTCCGTGG
    TGTCATCGTG CCCAGAAATT GGAAACAAGT TATGAACCCA AAATGGGTTT GACGACCTTC
    AAAGTTGTCC CTCCCAAACC TGAAGTAAAA AATTTTGATA GAGGGCTTTC AGTCTCCACT
    GGTGCTATTA AGATTGATGA ACTAGGCAAC CTTATCAACC CAAAGCCTGT TGTCAGTAAG
    AACATACCAG GTACCTCTGC TGGTGAAAGT GAGGAAATTC CTCTTGGGAA AGTGAAGGCA
    TTCTGGAGGT CAAGTTCTAT GGAAAAACAA AGTGATGACT CTGCTGAGCA TCTTGCTAAA
    AAGCCAGCAG TTACCACAAA CTCTAAGTCC TTTGTTACAA AGCAAGAATG CAAACCTGTC
    TGTCTTGCAG CTCCAAAACC TGTACCACCA CAAACTGTTA TTTCCCAGGT AGGTGAAAAG
    CAGTCTGAGA ATGAGAAGAC CAAACCACCT CTTCCAGTTA CACAGTCTCA GGTAAAACCA
    TTAGTGGTCC CAGCCATTAA TAAGGACAAG CCGGAGCTTC CATTTCTAAA GCCTCACAGA
    AGAACTTCCA GTCAGTATGT GGCCTCTGCC ATTGCAAAAC ACATCAATCT GCCTACCTTT
    AAGACTGATT CTACAGATTT GCATGAGAAA GATGAAAATA AGCACGGGGC AGGAGAGGTT
    AAAACTGAAG CTGAAGTTTC CCCCAAAAGG TGTATTATTG TGGCCAAGTA CAGCCCTGTG
    GAAACAGAAT CAGCAGAAAC AAGAGAAAAA ATTTCAAGTA TTTTTACTTG TAGTCAAAAA
    GCATCCCACA GATTCACACC TGGTGATAAT TCTGGCATGG AAAACAAAGT AGTGAACATC
    AGGGCACCGA GTCAAGCAAC TCCTGCTAGT TTCTATAAAA AGAATGCCAG TGTCCCACTT
    ACTAAATCCT CTTCAAAGGA TAACAACAAA GCAGAAGATG ATCACAGTTT AAACAGCAAA
    CAAGGTACAG CAGAGAAAAA GATGCATCTT TTGTTTGACC AGAAATACGA GACTTTGCCC
    CGTAGTAATT CAGCCTCCTC TCTCAAGTCA CCTGATCTCC AAGGAGCCTC ATCTTCTTTC
    AGTTCATCTT TGCGCATCAC TAAATGGCCT CCTGCTGACA GCGTGCAAGT TAGTTCCCTG
    AAAGCATCGC CCGAGTTCAG TGGTGTGACA GCAAAGGCAG AATCAGAACA GAATGACAAA
    TCTGATGATT CGGCTGCTAA TGCTATTGGC GAATTGGATG TTAATGTGCA GACTAATATC
    TTCGGACCAA AGAAGAAGTT CAAACCTGTT GTCCAAAAAC CAATTCCGAA AGATACATCA
    CTGCACAGTG CCCTCATGGA AGCTATTCAA ACAGGAGGGG GAAAAGAGAA ACTCAGAAAG
    GTTTCAGACA GCGCGCTAAA TGGCAATCAC AAGAAACCAT CATACAGTGA GCCAGAGAAT
    GAGCGGTCGG CCCTACTAGC GGCAATTAGA GGCCACAGTG GCACTTGCAA GCTGAAAAAA
    ATCTCCTCAT TGGCCTCCGA AGAGCTCCAG AGTTTTAGGA ATGCAGAACT GTCTTCACAG
    AAAGCAGAAG ACCCTCAGGA AGAGCAGCTC TGCATTCCAC CTGCCCCTGC CCAGCCACCA
    CCACCGCCTC CCCCTCAGCT GTCAACAGCA ACTTTCAAAT TCTCTGCCAC AACTACGGGT
    GACCCTGGAG AGGCCAGGCG AGCCCTGATG GAGGCCATTC GCTCTGGTGC TGGAGCAGCA
    AAGTTAAAAA AGGTCCCTCT GCTCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa04923
    Clone ID Related Accession (Same CDS sequence) XM_004935146.2 , XM_004935146.3
    Accession Version XM_004935146.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4239bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product protein cordon-bleu isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455789.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004935146.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAGACTC TCGTCCGCCC CACGGCCGGC AAGCCGCCCA GCGGGAGAAG AATGAAAGCT 
    CGTGCTCCTC CTCCACCAAA CCAACCTTCT GCAACCAGTA GAATTCACAG TGAGCACAAG
    TCACCTGCAG AGACAGCCGT AATTTCTGAT CAGAGCCTTG TGAGCATGAA GGAGAATATG
    ATAAATAGAA TGGTGGACTT CACAGTCATT TTACCCAGCG GGGTGGAACA GAAGAGCACA
    GTACAAGGAA GTAAAGCTGT GATGGATCTT TTGGTTGATC TATGCAGCCG GTATCACTTG
    AATCCATCCC AGTACAGTCT TGAATTGAAG TCTTTGGAAA CTCAACAACC TTTGAGTTAC
    AAGCCAAACA CTTTGATAGG AGCACTGGAC GTACAGACAG TACTCCTGAA AGAGAAGGTT
    CCTGAAGAGA AGATCAAGAG ACCTCTGCCG AGGGCTCCTG AGAAGTCTGT GAGGCTGGTG
    GTAAATTACC TGAAGACACA GAAGGCAGTG GTTCGAGTCA GCCCGGACGT GCCCCTTCAC
    AACATCATCC CAGCTATTTG TGAAAAGTGT GAGGTCAGTC AGGAGCACAT TGTTCTTCTG
    CGAGACAGCA TCACTGGCGA GGAGCTGGAG CTTACCAAAT CCTTGGAGGA GCTGGGAATA
    AGCGAGCTGT ACGCCTGGGA CCGAAAAAGA GTTCCTCCAT CAAAAACTCA GTCTGACCCT
    TCTCTGAACT ACAGAGAACC AAGTCGAAGT GTTTCACTAA GCAACGATGC AATAGAAAAA
    GAAAAGAAAG GATTTCTGGG GTTTTTCAAA GCTAATAGAA GCAACAGCAA GACAGAAGAA
    CACTTGAGGA TGAACAGAGA TTATGGTGAG GAGGATGTTT TTAGTTCTAC ATCTACAAGT
    GAAGGAAGCT TGGATGGTTT TTCCACAGCA CCAAACTCCC CTTCGGTGAA TTCCCGTTCC
    AGTACCTTGG GTCCATCGCT GTCCCTTGGT AATATCTCAG GAATGACAGC AAACCCAGAA
    GTGAAAAAGC GCAGAGCACC TCCTCCACCA GTTTTGACAC CTCCGTTGCA GAATATGGAG
    ATGAGCTCCG CTCAGGAGAA GACAGCAGTA CAGATTTCAC AAGGAGCATC TCTTAACGAT
    CTACAGAAAA AGAAGAGGCG TGCACCTCTT CCACCAACTC AGTCTGCTCC ACCTACTCCA
    GCCATGCCAA ATAGGAGGGA AGAGATGGAA GACAAGAGGA AGAGCACAAT GGGTGATGGA
    CGGCAGGTGC CACAAAAGCC TCCTAGGGGC CCCACTCGTG ACCCACCCCA GTTAGTGATC
    CCCCCACCCC CACCTTACCC TCCTCCTGAA AGAGACAATA TGGATCCAGC AGTCTCTTAC
    AGAGAAGCAG ATGTTACAGC ACCAACAAAA CTGGTACCTA AACAGAGCCT GCTGCCTGCA
    CATGATAATT TTTATGTAGT GGATGATACA GTTATGGAGC TATCAGAGGT AGAAGAAACA
    GCGTCAGAAA GCAGCTGCTT TGCATCTGAG GACACTACCG AAGACTCAGG TGTAGTGAGC
    TCCCCATCTG ACATTGTGTC ACTGGATTCA CAAAATGACA GTATGAAGTT GAGAGACATC
    AAGCTGGTGA ATGGCTATGT GGACTCAGAC ACGATCTATG GTGCAGAGAC TTGCTCTGTG
    AAGAACGCGT ACTGCGATGA TATGGGATAT GCCGGTACTC CACAGAGCAC TAAAAATGAA
    GAAATGGCTG CGGCTAAGCA TGAGAATGAG GACATATTCA TTGATGCACA ACTCCAGCAG
    ACTTTGGCTG ACCTGGATGA AGATTTAGAA GCTATGGATG TTTTAAGTAA CTCTGACTCT
    GAGCACATGA GTGAAGTGTT GAGCCCACAT TCAGGAAAAG TTGAGGTCCC CCAAGATGTT
    GCTAGTTCTG TTCCAGTAAC AGTCATAGAT GATGCTCCAG AAGTTACCAT CTATAACTCA
    GCTGGAAACC AAGAGACAAG GGGTTCACAG AATCTATCAG CTGACTCTGT TAATATGGGA
    AACTTCACAA ATAGAAACAA CAATGCAGGT TCCTTTGATA AGGGACACAC TGATGGTGGA
    GCTGTATGTA TACCCAAGGA CCTAATACAT CAGCAGCCAT CTGATAATGA GTTCAGTCAC
    ATACCCGTGG AACAGAGGAG AGATATGTTT GAATCTCTCA CGTCCTCCTT TGAAAAAAGA
    AGTGAAAAGA ATGTAAGAGT AGAAGCTCCG CCCAGACACG TTATGAAGTC TGAGGAGTGT
    AAATCGAAGC TGACGTCATC TCACTCCAAG ACTGCGACTG AAATCAATAC AGCAAAAGAG
    AAAGTAAATC CATTTAATGA ATGTAGTAAG AGGGAGAGAC CTCTGAAAAA AAGTAAGTCA
    GAACTAGAAA TCAAAAGGCC ACCAGCAAAA AAGATTGAAG TAGAAGAAGT TCCATCAACA
    CCTCCGTGGT GTCATCGTGC CCAGAAATTG GAAACAAGTT ATGAACCCAA AATGGGTTTG
    ACGACCTTCA AAGTTGTCCC TCCCAAACCT GAAGTAAAAA ATTTTGATAG AGGGCTTTCA
    GTCTCCACTG GTGCTATTAA GATTGATGAA CTAGGCAACC TTATCAACCC AAAGCCTGTT
    GTCAGTAAGA ACATACCAGG TACCTCTGCT GGTGAAAGTG AGGAAATTCC TCTTGGGAAA
    GTGAAGGCAT TCTGGAGGTC AAGTTCTATG GAAAAACAAA GTGATGACTC TGCTGAGCAT
    CTTGCTAAAA AGCCAGCAGT TACCACAAAC TCTAAGTCCT TTGTTACAAA GCAAGAATGC
    AAACCTGTCT GTCTTGCAGC TCCAAAACCT GTACCACCAC AAACTGTTAT TTCCCAGGTA
    GGTGAAAAGC AGTCTGAGAA TGAGAAGACC AAACCACCTC TTCCAGTTAC ACAGTCTCAG
    GTAAAACCAT TAGTGGTCCC AGCCATTAAT AAGGACAAGC CGGAGCTTCC ATTTCTAAAG
    CCTCACAGAA GAACTTCCAG TCAGTATGTG GCCTCTGCCA TTGCAAAACA CATCAATCTG
    CCTACCTTTA AGACTGATTC TACAGATTTG CATGAGAAAG ATGAAAATAA GCACGGGGCA
    GGAGAGGTTA AAACTGAAGC TGAAGTTTCC CCCAAAAGGT GTATTATTGT GGCCAAGTAC
    AGCCCTGTGG AAACAGAATC AGCAGAAACA AGAGAAAAAA TTTCAAGTAT TTTTACTTGT
    AGTCAAAAAG CATCCCACAG ATTCACACCT GGTGATAATT CTGGCATGGA AAACAAAGTA
    GTGAACATCA GGGCACCGAG TCAAGCAACT CCTGCTAGTT TCTATAAAAA GAATGCCAGT
    GTCCCACTTA CTAAATCCTC TTCAAAGGAT AACAACAAAG CAGAAGATGA TCACAGTTTA
    AACAGCAAAC AAGGTACAGC AGAGAAAAAG ATGCATCTTT TGTTTGACCA GAAATACGAG
    ACTTTGCCCC GTAGTAATTC AGCCTCCTCT CTCAAGTCAC CTGATCTCCA AGGAGCCTCA
    TCTTCTTTCA GTTCATCTTT GCGCATCACT AAATGGCCTC CTGCTGACAG CGTGCAAGTT
    AGTTCCCTGA AAGCATCGCC CGAGTTCAGT GGTGTGACAG CAAAGGCAGA ATCAGAACAG
    AATGACAAAT CTGATGATTC GGCTGCTAAT GCTATTGGCG AATTGGATGT TAATGTGCAG
    ACTAATATCT TCGGACCAAA GAAGAAGTTC AAACCTGTTG TCCAAAAACC AATTCCGAAA
    GATACATCAC TGCACAGTGC CCTCATGGAA GCTATTCAAA CAGGAGGGGG AAAAGAGAAA
    CTCAGAAAGG TTTCAGACAG CGCGCTAAAT GGCAATCACA AGAAACCATC ATACAGTGAG
    CCAGAGAATG AGCGGTCGGC CCTACTAGCG GCAATTAGAG GCCACAGTGG CACTTGCAAG
    CTGAAAAAAA TCTCCTCATT GGCCTCCGAA GAGCTCCAGA GTTTTAGGAA TGCAGAACTG
    TCTTCACAGA AAGCAGAAGA CCCTCAGGAA GAGCAGCTCT GCATTCCACC TGCCCCTGCC
    CAGCCACCAC CACCGCCTCC CCCTCAGCTG TCAACAGCAA CTTTCAAATT CTCTGCCACA
    ACTACGGGTG ACCCTGGAGA GGCCAGGCGA GCCCTGATGG AGGCCATTCG CTCTGGTGCT
    GGAGCAGCAA AGTTAAAAAA GGTCCCTCTG CTCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004935203.1
    CDS194..4432
    Translation

    Target ORF information:

    RefSeq Version XM_004935146.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004935146.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAGACTC TCGTCCGCCC CACGGCCGGC AAGCCGCCCA GCGGGAGAAG AATGAAAGCT 
    CGTGCTCCTC CTCCACCAAA CCAACCTTCT GCAACCAGTA GAATTCACAG TGAGCACAAG
    TCACCTGCAG AGACAGCCGT AATTTCTGAT CAGAGCCTTG TGAGCATGAA GGAGAATATG
    ATAAATAGAA TGGTGGACTT CACAGTCATT TTACCCAGCG GGGTGGAACA GAAGAGCACA
    GTACAAGGAA GTAAAGCTGT GATGGATCTT TTGGTTGATC TATGCAGCCG GTATCACTTG
    AATCCATCCC AGTACAGTCT TGAATTGAAG TCTTTGGAAA CTCAACAACC TTTGAGTTAC
    AAGCCAAACA CTTTGATAGG AGCACTGGAC GTACAGACAG TACTCCTGAA AGAGAAGGTT
    CCTGAAGAGA AGATCAAGAG ACCTCTGCCG AGGGCTCCTG AGAAGTCTGT GAGGCTGGTG
    GTAAATTACC TGAAGACACA GAAGGCAGTG GTTCGAGTCA GCCCGGACGT GCCCCTTCAC
    AACATCATCC CAGCTATTTG TGAAAAGTGT GAGGTCAGTC AGGAGCACAT TGTTCTTCTG
    CGAGACAGCA TCACTGGCGA GGAGCTGGAG CTTACCAAAT CCTTGGAGGA GCTGGGAATA
    AGCGAGCTGT ACGCCTGGGA CCGAAAAAGA GTTCCTCCAT CAAAAACTCA GTCTGACCCT
    TCTCTGAACT ACAGAGAACC AAGTCGAAGT GTTTCACTAA GCAACGATGC AATAGAAAAA
    GAAAAGAAAG GATTTCTGGG GTTTTTCAAA GCTAATAGAA GCAACAGCAA GACAGAAGAA
    CACTTGAGGA TGAACAGAGA TTATGGTGAG GAGGATGTTT TTAGTTCTAC ATCTACAAGT
    GAAGGAAGCT TGGATGGTTT TTCCACAGCA CCAAACTCCC CTTCGGTGAA TTCCCGTTCC
    AGTACCTTGG GTCCATCGCT GTCCCTTGGT AATATCTCAG GAATGACAGC AAACCCAGAA
    GTGAAAAAGC GCAGAGCACC TCCTCCACCA GTTTTGACAC CTCCGTTGCA GAATATGGAG
    ATGAGCTCCG CTCAGGAGAA GACAGCAGTA CAGATTTCAC AAGGAGCATC TCTTAACGAT
    CTACAGAAAA AGAAGAGGCG TGCACCTCTT CCACCAACTC AGTCTGCTCC ACCTACTCCA
    GCCATGCCAA ATAGGAGGGA AGAGATGGAA GACAAGAGGA AGAGCACAAT GGGTGATGGA
    CGGCAGGTGC CACAAAAGCC TCCTAGGGGC CCCACTCGTG ACCCACCCCA GTTAGTGATC
    CCCCCACCCC CACCTTACCC TCCTCCTGAA AGAGACAATA TGGATCCAGC AGTCTCTTAC
    AGAGAAGCAG ATGTTACAGC ACCAACAAAA CTGGTACCTA AACAGAGCCT GCTGCCTGCA
    CATGATAATT TTTATGTAGT GGATGATACA GTTATGGAGC TATCAGAGGT AGAAGAAACA
    GCGTCAGAAA GCAGCTGCTT TGCATCTGAG GACACTACCG AAGACTCAGG TGTAGTGAGC
    TCCCCATCTG ACATTGTGTC ACTGGATTCA CAAAATGACA GTATGAAGTT GAGAGACATC
    AAGCTGGTGA ATGGCTATGT GGACTCAGAC ACGATCTATG GTGCAGAGAC TTGCTCTGTG
    AAGAACGCGT ACTGCGATGA TATGGGATAT GCCGGTACTC CACAGAGCAC TAAAAATGAA
    GAAATGGCTG CGGCTAAGCA TGAGAATGAG GACATATTCA TTGATGCACA ACTCCAGCAG
    ACTTTGGCTG ACCTGGATGA AGATTTAGAA GCTATGGATG TTTTAAGTAA CTCTGACTCT
    GAGCACATGA GTGAAGTGTT GAGCCCACAT TCAGGAAAAG TTGAGGTCCC CCAAGATGTT
    GCTAGTTCTG TTCCAGTAAC AGTCATAGAT GATGCTCCAG AAGTTACCAT CTATAACTCA
    GCTGGAAACC AAGAGACAAG GGGTTCACAG AATCTATCAG CTGACTCTGT TAATATGGGA
    AACTTCACAA ATAGAAACAA CAATGCAGGT TCCTTTGATA AGGGACACAC TGATGGTGGA
    GCTGTATGTA TACCCAAGGA CCTAATACAT CAGCAGCCAT CTGATAATGA GTTCAGTCAC
    ATACCCGTGG AACAGAGGAG AGATATGTTT GAATCTCTCA CGTCCTCCTT TGAAAAAAGA
    AGTGAAAAGA ATGTAAGAGT AGAAGCTCCG CCCAGACACG TTATGAAGTC TGAGGAGTGT
    AAATCGAAGC TGACGTCATC TCACTCCAAG ACTGCGACTG AAATCAATAC AGCAAAAGAG
    AAAGTAAATC CATTTAATGA ATGTAGTAAG AGGGAGAGAC CTCTGAAAAA AAGTAAGTCA
    GAACTAGAAA TCAAAAGGCC ACCAGCAAAA AAGATTGAAG TAGAAGAAGT TCCATCAACA
    CCTCCGTGGT GTCATCGTGC CCAGAAATTG GAAACAAGTT ATGAACCCAA AATGGGTTTG
    ACGACCTTCA AAGTTGTCCC TCCCAAACCT GAAGTAAAAA ATTTTGATAG AGGGCTTTCA
    GTCTCCACTG GTGCTATTAA GATTGATGAA CTAGGCAACC TTATCAACCC AAAGCCTGTT
    GTCAGTAAGA ACATACCAGG TACCTCTGCT GGTGAAAGTG AGGAAATTCC TCTTGGGAAA
    GTGAAGGCAT TCTGGAGGTC AAGTTCTATG GAAAAACAAA GTGATGACTC TGCTGAGCAT
    CTTGCTAAAA AGCCAGCAGT TACCACAAAC TCTAAGTCCT TTGTTACAAA GCAAGAATGC
    AAACCTGTCT GTCTTGCAGC TCCAAAACCT GTACCACCAC AAACTGTTAT TTCCCAGGTA
    GGTGAAAAGC AGTCTGAGAA TGAGAAGACC AAACCACCTC TTCCAGTTAC ACAGTCTCAG
    GTAAAACCAT TAGTGGTCCC AGCCATTAAT AAGGACAAGC CGGAGCTTCC ATTTCTAAAG
    CCTCACAGAA GAACTTCCAG TCAGTATGTG GCCTCTGCCA TTGCAAAACA CATCAATCTG
    CCTACCTTTA AGACTGATTC TACAGATTTG CATGAGAAAG ATGAAAATAA GCACGGGGCA
    GGAGAGGTTA AAACTGAAGC TGAAGTTTCC CCCAAAAGGT GTATTATTGT GGCCAAGTAC
    AGCCCTGTGG AAACAGAATC AGCAGAAACA AGAGAAAAAA TTTCAAGTAT TTTTACTTGT
    AGTCAAAAAG CATCCCACAG ATTCACACCT GGTGATAATT CTGGCATGGA AAACAAAGTA
    GTGAACATCA GGGCACCGAG TCAAGCAACT CCTGCTAGTT TCTATAAAAA GAATGCCAGT
    GTCCCACTTA CTAAATCCTC TTCAAAGGAT AACAACAAAG CAGAAGATGA TCACAGTTTA
    AACAGCAAAC AAGGTACAGC AGAGAAAAAG ATGCATCTTT TGTTTGACCA GAAATACGAG
    ACTTTGCCCC GTAGTAATTC AGCCTCCTCT CTCAAGTCAC CTGATCTCCA AGGAGCCTCA
    TCTTCTTTCA GTTCATCTTT GCGCATCACT AAATGGCCTC CTGCTGACAG CGTGCAAGTT
    AGTTCCCTGA AAGCATCGCC CGAGTTCAGT GGTGTGACAG CAAAGGCAGA ATCAGAACAG
    AATGACAAAT CTGATGATTC GGCTGCTAAT GCTATTGGCG AATTGGATGT TAATGTGCAG
    ACTAATATCT TCGGACCAAA GAAGAAGTTC AAACCTGTTG TCCAAAAACC AATTCCGAAA
    GATACATCAC TGCACAGTGC CCTCATGGAA GCTATTCAAA CAGGAGGGGG AAAAGAGAAA
    CTCAGAAAGG TTTCAGACAG CGCGCTAAAT GGCAATCACA AGAAACCATC ATACAGTGAG
    CCAGAGAATG AGCGGTCGGC CCTACTAGCG GCAATTAGAG GCCACAGTGG CACTTGCAAG
    CTGAAAAAAA TCTCCTCATT GGCCTCCGAA GAGCTCCAGA GTTTTAGGAA TGCAGAACTG
    TCTTCACAGA AAGCAGAAGA CCCTCAGGAA GAGCAGCTCT GCATTCCACC TGCCCCTGCC
    CAGCCACCAC CACCGCCTCC CCCTCAGCTG TCAACAGCAA CTTTCAAATT CTCTGCCACA
    ACTACGGGTG ACCCTGGAGA GGCCAGGCGA GCCCTGATGG AGGCCATTCG CTCTGGTGCT
    GGAGCAGCAA AGTTAAAAAA GGTCCCTCTG CTCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa04923
    Clone ID Related Accession (Same CDS sequence) XM_004935146.2 , XM_004935146.3
    Accession Version XM_004935146.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4239bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein cordon-bleu isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004935146.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAGACTC TCGTCCGCCC CACGGCCGGC AAGCCGCCCA GCGGGAGAAG AATGAAAGCT 
    CGTGCTCCTC CTCCACCAAA CCAACCTTCT GCAACCAGTA GAATTCACAG TGAGCACAAG
    TCACCTGCAG AGACAGCCGT AATTTCTGAT CAGAGCCTTG TGAGCATGAA GGAGAATATG
    ATAAATAGAA TGGTGGACTT CACAGTCATT TTACCCAGCG GGGTGGAACA GAAGAGCACA
    GTACAAGGAA GTAAAGCTGT GATGGATCTT TTGGTTGATC TATGCAGCCG GTATCACTTG
    AATCCATCCC AGTACAGTCT TGAATTGAAG TCTTTGGAAA CTCAACAACC TTTGAGTTAC
    AAGCCAAACA CTTTGATAGG AGCACTGGAC GTACAGACAG TACTCCTGAA AGAGAAGGTT
    CCTGAAGAGA AGATCAAGAG ACCTCTGCCG AGGGCTCCTG AGAAGTCTGT GAGGCTGGTG
    GTAAATTACC TGAAGACACA GAAGGCAGTG GTTCGAGTCA GCCCGGACGT GCCCCTTCAC
    AACATCATCC CAGCTATTTG TGAAAAGTGT GAGGTCAGTC AGGAGCACAT TGTTCTTCTG
    CGAGACAGCA TCACTGGCGA GGAGCTGGAG CTTACCAAAT CCTTGGAGGA GCTGGGAATA
    AGCGAGCTGT ACGCCTGGGA CCGAAAAAGA GTTCCTCCAT CAAAAACTCA GTCTGACCCT
    TCTCTGAACT ACAGAGAACC AAGTCGAAGT GTTTCACTAA GCAACGATGC AATAGAAAAA
    GAAAAGAAAG GATTTCTGGG GTTTTTCAAA GCTAATAGAA GCAACAGCAA GACAGAAGAA
    CACTTGAGGA TGAACAGAGA TTATGGTGAG GAGGATGTTT TTAGTTCTAC ATCTACAAGT
    GAAGGAAGCT TGGATGGTTT TTCCACAGCA CCAAACTCCC CTTCGGTGAA TTCCCGTTCC
    AGTACCTTGG GTCCATCGCT GTCCCTTGGT AATATCTCAG GAATGACAGC AAACCCAGAA
    GTGAAAAAGC GCAGAGCACC TCCTCCACCA GTTTTGACAC CTCCGTTGCA GAATATGGAG
    ATGAGCTCCG CTCAGGAGAA GACAGCAGTA CAGATTTCAC AAGGAGCATC TCTTAACGAT
    CTACAGAAAA AGAAGAGGCG TGCACCTCTT CCACCAACTC AGTCTGCTCC ACCTACTCCA
    GCCATGCCAA ATAGGAGGGA AGAGATGGAA GACAAGAGGA AGAGCACAAT GGGTGATGGA
    CGGCAGGTGC CACAAAAGCC TCCTAGGGGC CCCACTCGTG ACCCACCCCA GTTAGTGATC
    CCCCCACCCC CACCTTACCC TCCTCCTGAA AGAGACAATA TGGATCCAGC AGTCTCTTAC
    AGAGAAGCAG ATGTTACAGC ACCAACAAAA CTGGTACCTA AACAGAGCCT GCTGCCTGCA
    CATGATAATT TTTATGTAGT GGATGATACA GTTATGGAGC TATCAGAGGT AGAAGAAACA
    GCGTCAGAAA GCAGCTGCTT TGCATCTGAG GACACTACCG AAGACTCAGG TGTAGTGAGC
    TCCCCATCTG ACATTGTGTC ACTGGATTCA CAAAATGACA GTATGAAGTT GAGAGACATC
    AAGCTGGTGA ATGGCTATGT GGACTCAGAC ACGATCTATG GTGCAGAGAC TTGCTCTGTG
    AAGAACGCGT ACTGCGATGA TATGGGATAT GCCGGTACTC CACAGAGCAC TAAAAATGAA
    GAAATGGCTG CGGCTAAGCA TGAGAATGAG GACATATTCA TTGATGCACA ACTCCAGCAG
    ACTTTGGCTG ACCTGGATGA AGATTTAGAA GCTATGGATG TTTTAAGTAA CTCTGACTCT
    GAGCACATGA GTGAAGTGTT GAGCCCACAT TCAGGAAAAG TTGAGGTCCC CCAAGATGTT
    GCTAGTTCTG TTCCAGTAAC AGTCATAGAT GATGCTCCAG AAGTTACCAT CTATAACTCA
    GCTGGAAACC AAGAGACAAG GGGTTCACAG AATCTATCAG CTGACTCTGT TAATATGGGA
    AACTTCACAA ATAGAAACAA CAATGCAGGT TCCTTTGATA AGGGACACAC TGATGGTGGA
    GCTGTATGTA TACCCAAGGA CCTAATACAT CAGCAGCCAT CTGATAATGA GTTCAGTCAC
    ATACCCGTGG AACAGAGGAG AGATATGTTT GAATCTCTCA CGTCCTCCTT TGAAAAAAGA
    AGTGAAAAGA ATGTAAGAGT AGAAGCTCCG CCCAGACACG TTATGAAGTC TGAGGAGTGT
    AAATCGAAGC TGACGTCATC TCACTCCAAG ACTGCGACTG AAATCAATAC AGCAAAAGAG
    AAAGTAAATC CATTTAATGA ATGTAGTAAG AGGGAGAGAC CTCTGAAAAA AAGTAAGTCA
    GAACTAGAAA TCAAAAGGCC ACCAGCAAAA AAGATTGAAG TAGAAGAAGT TCCATCAACA
    CCTCCGTGGT GTCATCGTGC CCAGAAATTG GAAACAAGTT ATGAACCCAA AATGGGTTTG
    ACGACCTTCA AAGTTGTCCC TCCCAAACCT GAAGTAAAAA ATTTTGATAG AGGGCTTTCA
    GTCTCCACTG GTGCTATTAA GATTGATGAA CTAGGCAACC TTATCAACCC AAAGCCTGTT
    GTCAGTAAGA ACATACCAGG TACCTCTGCT GGTGAAAGTG AGGAAATTCC TCTTGGGAAA
    GTGAAGGCAT TCTGGAGGTC AAGTTCTATG GAAAAACAAA GTGATGACTC TGCTGAGCAT
    CTTGCTAAAA AGCCAGCAGT TACCACAAAC TCTAAGTCCT TTGTTACAAA GCAAGAATGC
    AAACCTGTCT GTCTTGCAGC TCCAAAACCT GTACCACCAC AAACTGTTAT TTCCCAGGTA
    GGTGAAAAGC AGTCTGAGAA TGAGAAGACC AAACCACCTC TTCCAGTTAC ACAGTCTCAG
    GTAAAACCAT TAGTGGTCCC AGCCATTAAT AAGGACAAGC CGGAGCTTCC ATTTCTAAAG
    CCTCACAGAA GAACTTCCAG TCAGTATGTG GCCTCTGCCA TTGCAAAACA CATCAATCTG
    CCTACCTTTA AGACTGATTC TACAGATTTG CATGAGAAAG ATGAAAATAA GCACGGGGCA
    GGAGAGGTTA AAACTGAAGC TGAAGTTTCC CCCAAAAGGT GTATTATTGT GGCCAAGTAC
    AGCCCTGTGG AAACAGAATC AGCAGAAACA AGAGAAAAAA TTTCAAGTAT TTTTACTTGT
    AGTCAAAAAG CATCCCACAG ATTCACACCT GGTGATAATT CTGGCATGGA AAACAAAGTA
    GTGAACATCA GGGCACCGAG TCAAGCAACT CCTGCTAGTT TCTATAAAAA GAATGCCAGT
    GTCCCACTTA CTAAATCCTC TTCAAAGGAT AACAACAAAG CAGAAGATGA TCACAGTTTA
    AACAGCAAAC AAGGTACAGC AGAGAAAAAG ATGCATCTTT TGTTTGACCA GAAATACGAG
    ACTTTGCCCC GTAGTAATTC AGCCTCCTCT CTCAAGTCAC CTGATCTCCA AGGAGCCTCA
    TCTTCTTTCA GTTCATCTTT GCGCATCACT AAATGGCCTC CTGCTGACAG CGTGCAAGTT
    AGTTCCCTGA AAGCATCGCC CGAGTTCAGT GGTGTGACAG CAAAGGCAGA ATCAGAACAG
    AATGACAAAT CTGATGATTC GGCTGCTAAT GCTATTGGCG AATTGGATGT TAATGTGCAG
    ACTAATATCT TCGGACCAAA GAAGAAGTTC AAACCTGTTG TCCAAAAACC AATTCCGAAA
    GATACATCAC TGCACAGTGC CCTCATGGAA GCTATTCAAA CAGGAGGGGG AAAAGAGAAA
    CTCAGAAAGG TTTCAGACAG CGCGCTAAAT GGCAATCACA AGAAACCATC ATACAGTGAG
    CCAGAGAATG AGCGGTCGGC CCTACTAGCG GCAATTAGAG GCCACAGTGG CACTTGCAAG
    CTGAAAAAAA TCTCCTCATT GGCCTCCGAA GAGCTCCAGA GTTTTAGGAA TGCAGAACTG
    TCTTCACAGA AAGCAGAAGA CCCTCAGGAA GAGCAGCTCT GCATTCCACC TGCCCCTGCC
    CAGCCACCAC CACCGCCTCC CCCTCAGCTG TCAACAGCAA CTTTCAAATT CTCTGCCACA
    ACTACGGGTG ACCCTGGAGA GGCCAGGCGA GCCCTGATGG AGGCCATTCG CTCTGGTGCT
    GGAGCAGCAA AGTTAAAAAA GGTCCCTCTG CTCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004935203.1
    CDS369..4607
    Translation

    Target ORF information:

    RefSeq Version XM_004935146.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus cordon-bleu WH2 repeat protein (COBL), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004935146.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAGACTC TCGTCCGCCC CACGGCCGGC AAGCCGCCCA GCGGGAGAAG AATGAAAGCT 
    CGTGCTCCTC CTCCACCAAA CCAACCTTCT GCAACCAGTA GAATTCACAG TGAGCACAAG
    TCACCTGCAG AGACAGCCGT AATTTCTGAT CAGAGCCTTG TGAGCATGAA GGAGAATATG
    ATAAATAGAA TGGTGGACTT CACAGTCATT TTACCCAGCG GGGTGGAACA GAAGAGCACA
    GTACAAGGAA GTAAAGCTGT GATGGATCTT TTGGTTGATC TATGCAGCCG GTATCACTTG
    AATCCATCCC AGTACAGTCT TGAATTGAAG TCTTTGGAAA CTCAACAACC TTTGAGTTAC
    AAGCCAAACA CTTTGATAGG AGCACTGGAC GTACAGACAG TACTCCTGAA AGAGAAGGTT
    CCTGAAGAGA AGATCAAGAG ACCTCTGCCG AGGGCTCCTG AGAAGTCTGT GAGGCTGGTG
    GTAAATTACC TGAAGACACA GAAGGCAGTG GTTCGAGTCA GCCCGGACGT GCCCCTTCAC
    AACATCATCC CAGCTATTTG TGAAAAGTGT GAGGTCAGTC AGGAGCACAT TGTTCTTCTG
    CGAGACAGCA TCACTGGCGA GGAGCTGGAG CTTACCAAAT CCTTGGAGGA GCTGGGAATA
    AGCGAGCTGT ACGCCTGGGA CCGAAAAAGA GTTCCTCCAT CAAAAACTCA GTCTGACCCT
    TCTCTGAACT ACAGAGAACC AAGTCGAAGT GTTTCACTAA GCAACGATGC AATAGAAAAA
    GAAAAGAAAG GATTTCTGGG GTTTTTCAAA GCTAATAGAA GCAACAGCAA GACAGAAGAA
    CACTTGAGGA TGAACAGAGA TTATGGTGAG GAGGATGTTT TTAGTTCTAC ATCTACAAGT
    GAAGGAAGCT TGGATGGTTT TTCCACAGCA CCAAACTCCC CTTCGGTGAA TTCCCGTTCC
    AGTACCTTGG GTCCATCGCT GTCCCTTGGT AATATCTCAG GAATGACAGC AAACCCAGAA
    GTGAAAAAGC GCAGAGCACC TCCTCCACCA GTTTTGACAC CTCCGTTGCA GAATATGGAG
    ATGAGCTCCG CTCAGGAGAA GACAGCAGTA CAGATTTCAC AAGGAGCATC TCTTAACGAT
    CTACAGAAAA AGAAGAGGCG TGCACCTCTT CCACCAACTC AGTCTGCTCC ACCTACTCCA
    GCCATGCCAA ATAGGAGGGA AGAGATGGAA GACAAGAGGA AGAGCACAAT GGGTGATGGA
    CGGCAGGTGC CACAAAAGCC TCCTAGGGGC CCCACTCGTG ACCCACCCCA GTTAGTGATC
    CCCCCACCCC CACCTTACCC TCCTCCTGAA AGAGACAATA TGGATCCAGC AGTCTCTTAC
    AGAGAAGCAG ATGTTACAGC ACCAACAAAA CTGGTACCTA AACAGAGCCT GCTGCCTGCA
    CATGATAATT TTTATGTAGT GGATGATACA GTTATGGAGC TATCAGAGGT AGAAGAAACA
    GCGTCAGAAA GCAGCTGCTT TGCATCTGAG GACACTACCG AAGACTCAGG TGTAGTGAGC
    TCCCCATCTG ACATTGTGTC ACTGGATTCA CAAAATGACA GTATGAAGTT GAGAGACATC
    AAGCTGGTGA ATGGCTATGT GGACTCAGAC ACGATCTATG GTGCAGAGAC TTGCTCTGTG
    AAGAACGCGT ACTGCGATGA TATGGGATAT GCCGGTACTC CACAGAGCAC TAAAAATGAA
    GAAATGGCTG CGGCTAAGCA TGAGAATGAG GACATATTCA TTGATGCACA ACTCCAGCAG
    ACTTTGGCTG ACCTGGATGA AGATTTAGAA GCTATGGATG TTTTAAGTAA CTCTGACTCT
    GAGCACATGA GTGAAGTGTT GAGCCCACAT TCAGGAAAAG TTGAGGTCCC CCAAGATGTT
    GCTAGTTCTG TTCCAGTAAC AGTCATAGAT GATGCTCCAG AAGTTACCAT CTATAACTCA
    GCTGGAAACC AAGAGACAAG GGGTTCACAG AATCTATCAG CTGACTCTGT TAATATGGGA
    AACTTCACAA ATAGAAACAA CAATGCAGGT TCCTTTGATA AGGGACACAC TGATGGTGGA
    GCTGTATGTA TACCCAAGGA CCTAATACAT CAGCAGCCAT CTGATAATGA GTTCAGTCAC
    ATACCCGTGG AACAGAGGAG AGATATGTTT GAATCTCTCA CGTCCTCCTT TGAAAAAAGA
    AGTGAAAAGA ATGTAAGAGT AGAAGCTCCG CCCAGACACG TTATGAAGTC TGAGGAGTGT
    AAATCGAAGC TGACGTCATC TCACTCCAAG ACTGCGACTG AAATCAATAC AGCAAAAGAG
    AAAGTAAATC CATTTAATGA ATGTAGTAAG AGGGAGAGAC CTCTGAAAAA AAGTAAGTCA
    GAACTAGAAA TCAAAAGGCC ACCAGCAAAA AAGATTGAAG TAGAAGAAGT TCCATCAACA
    CCTCCGTGGT GTCATCGTGC CCAGAAATTG GAAACAAGTT ATGAACCCAA AATGGGTTTG
    ACGACCTTCA AAGTTGTCCC TCCCAAACCT GAAGTAAAAA ATTTTGATAG AGGGCTTTCA
    GTCTCCACTG GTGCTATTAA GATTGATGAA CTAGGCAACC TTATCAACCC AAAGCCTGTT
    GTCAGTAAGA ACATACCAGG TACCTCTGCT GGTGAAAGTG AGGAAATTCC TCTTGGGAAA
    GTGAAGGCAT TCTGGAGGTC AAGTTCTATG GAAAAACAAA GTGATGACTC TGCTGAGCAT
    CTTGCTAAAA AGCCAGCAGT TACCACAAAC TCTAAGTCCT TTGTTACAAA GCAAGAATGC
    AAACCTGTCT GTCTTGCAGC TCCAAAACCT GTACCACCAC AAACTGTTAT TTCCCAGGTA
    GGTGAAAAGC AGTCTGAGAA TGAGAAGACC AAACCACCTC TTCCAGTTAC ACAGTCTCAG
    GTAAAACCAT TAGTGGTCCC AGCCATTAAT AAGGACAAGC CGGAGCTTCC ATTTCTAAAG
    CCTCACAGAA GAACTTCCAG TCAGTATGTG GCCTCTGCCA TTGCAAAACA CATCAATCTG
    CCTACCTTTA AGACTGATTC TACAGATTTG CATGAGAAAG ATGAAAATAA GCACGGGGCA
    GGAGAGGTTA AAACTGAAGC TGAAGTTTCC CCCAAAAGGT GTATTATTGT GGCCAAGTAC
    AGCCCTGTGG AAACAGAATC AGCAGAAACA AGAGAAAAAA TTTCAAGTAT TTTTACTTGT
    AGTCAAAAAG CATCCCACAG ATTCACACCT GGTGATAATT CTGGCATGGA AAACAAAGTA
    GTGAACATCA GGGCACCGAG TCAAGCAACT CCTGCTAGTT TCTATAAAAA GAATGCCAGT
    GTCCCACTTA CTAAATCCTC TTCAAAGGAT AACAACAAAG CAGAAGATGA TCACAGTTTA
    AACAGCAAAC AAGGTACAGC AGAGAAAAAG ATGCATCTTT TGTTTGACCA GAAATACGAG
    ACTTTGCCCC GTAGTAATTC AGCCTCCTCT CTCAAGTCAC CTGATCTCCA AGGAGCCTCA
    TCTTCTTTCA GTTCATCTTT GCGCATCACT AAATGGCCTC CTGCTGACAG CGTGCAAGTT
    AGTTCCCTGA AAGCATCGCC CGAGTTCAGT GGTGTGACAG CAAAGGCAGA ATCAGAACAG
    AATGACAAAT CTGATGATTC GGCTGCTAAT GCTATTGGCG AATTGGATGT TAATGTGCAG
    ACTAATATCT TCGGACCAAA GAAGAAGTTC AAACCTGTTG TCCAAAAACC AATTCCGAAA
    GATACATCAC TGCACAGTGC CCTCATGGAA GCTATTCAAA CAGGAGGGGG AAAAGAGAAA
    CTCAGAAAGG TTTCAGACAG CGCGCTAAAT GGCAATCACA AGAAACCATC ATACAGTGAG
    CCAGAGAATG AGCGGTCGGC CCTACTAGCG GCAATTAGAG GCCACAGTGG CACTTGCAAG
    CTGAAAAAAA TCTCCTCATT GGCCTCCGAA GAGCTCCAGA GTTTTAGGAA TGCAGAACTG
    TCTTCACAGA AAGCAGAAGA CCCTCAGGAA GAGCAGCTCT GCATTCCACC TGCCCCTGCC
    CAGCCACCAC CACCGCCTCC CCCTCAGCTG TCAACAGCAA CTTTCAAATT CTCTGCCACA
    ACTACGGGTG ACCCTGGAGA GGCCAGGCGA GCCCTGATGG AGGCCATTCG CTCTGGTGCT
    GGAGCAGCAA AGTTAAAAAA GGTCCCTCTG CTCGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.