PITRM1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	PITRM1
Entrez Gene ID	420462
Full Name	pitrilysin metallopeptidase 1
General protein information	Preferred Names pitrilysin metallopeptidase 1 Names presequence protease, mitochondrial
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_418564.4	XP_418564.2	presequence protease, mitochondrial
XM_418564.5	XP_418564.2	presequence protease, mitochondrial

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PITRM1	XP_001140483.1
Schizosaccharomyces pombe (fission yeast)	SPBC119.17	NP_595299.2
Magnaporthe oryzae (rice blast fungus)	MGG_02440	XP_003709229.1
Neurospora crassa	NCU01272	XP_960750.1
Arabidopsis thaliana (thale cress)	PREP1	NP_188548.2
Bos taurus (cattle)	PITRM1	XP_002692237.2
Gallus gallus (chicken)	PITRM1	XP_418564.2
Arabidopsis thaliana (thale cress)	PREP2	NP_850962.1
Homo sapiens (human)	PITRM1	NP_055704.2
Canis lupus familiaris (dog)	PITRM1	XP_535200.2
Rattus norvegicus (Norway rat)	Pitrm1	NP_001100833.1
	PITRM1	XP_001102165.1
Drosophila melanogaster (fruit fly)	CG3107	NP_001036470.1
Mus musculus (house mouse)	Pitrm1	NP_660113.1
Danio rerio (zebrafish)	pitrm1	NP_998652.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006616	XP_316646.4
Eremothecium gossypii	AGOS_ADR184W	NP_984280.2
Xenopus tropicalis (tropical clawed frog)	pitrm1	NP_001039262.1

Gene Ontology

The following PITRM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PITRM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa04027	XM_418564.5 Latest version!	Gallus gallus pitrilysin metallopeptidase 1 (PITRM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OGa04027	XM_418564.4	Gallus gallus pitrilysin metallopeptidase 1 (PITRM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PITRM1 ( XM_418564.4 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_418564.2 Gallus gallus pitrilysin metallopeptidase 1 (PITRM1), mRNA.

Price & Availability↑

CloneID	OGa04027
Clone ID Related Accession (Same CDS sequence)	XM_418564.4 , XM_418564.5
Accession Version	XM_418564.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	presequence protease, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_418564.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAGCC GCTACCGGGC GGCCGCCGGG CTGCTGAGCC GGGGAAGGTC TCTGAATCAG 
AAGTCATGGA GGTGGAAGAG CATTCCTGCC AACGAACGGG CGCTTCTTTA TAGGGTTGGA 
GAACAAATCC ATGGCTTCAC GGTAACTCAG GTTACAGCTG TTCCTGAGCT GTTCCTGACT 
GCAGTGAAAC TTAGTCATGA CAGTACAGGA GCCAGATATT TACACGTTGC TCGAGAGGAT 
TCCAATAATC TATTCAGTTT ACAATTCCGA ACCACTCCAA TGGACAGCAC TGGGGTCCCA 
CATATCCTTG AGCACACGGT GTTATGTGGT TCTCAGCAGT ATCCTTGCAG AGATCCGTTC 
TTTAAAATGT TAAACAGATC ATTGTCCACT TTCATGAATG CATTTACGGC TAGTGATTAC 
ACGCTCTATC CATTTTCAAC ACAAAATCCC AAGGATTTCC GGAATCTCCT GTCAGTGTAT 
TTGGATGCAG CTTTCTTTCC GTGTTTGCGG CAACTAGATT TCTGGCAAGA AGGTTGGAGG 
TTAGAACATG AGAACCCAAC TGATCCTCAG ACACCTCTAA TTTTCAAAGG AGTTGTTTTT 
AATGAAATGA AAGGGGCATT TACAGATAAC GAGAGGGTAT TTGCACAGCA CCTGCAGAAT 
AAACTCCTTC CTGATCACAC TTATGGTGTA GTATCTGGTG GACATCCCTT AAGTATTCCT 
GATCTTACAT GGGAGCAGCT TAAACAGTTC CATGCTTCAC ATTATCATCC CAGCAATTCC 
AGATTCTTCA CTTATGGTAA TTTTCCACTG GAGCATCACT TGAAACAAAT TCATGAAGAA 
GCACTGGTAA AATTTGAAAG AATTGAATCA AAGACTGACA TCCCAAAACA GAAAATCTGG 
GAAAAGCCTC AAGAACACCA TATAACATGT GGCTTAGACT CATTTGCTAG TGATCCTTCC 
AAGCAAACGA CTGTCAGCGT CAGTTACTTG TTGACAGACA TTACAGACGT TTTTGAAACT 
TTCACGCTAA GTCTCTTGTC TTCACTATTG GTTGATGGAC CAAATTCTCC ATTTTACAAA 
GCCTTAATTG AGTCTGGCGT TGGTACAGAT TTTTCTCCTG ATGTTGGGTT TAATGGCTCA 
ACAAGAGAAG CCTATTTCAG TGTGGGTCTG CAGGGTATTG CTGAGAGAGA CATTGAGACT 
GTGAAACAGA TTATTGCTAG AACAGTTGAT GAAGTTATTG CGAAAGGATT TGAAGAAGAT 
AGGATTGAAG CTCTACTTCA CAAAATTGAA ATCCAGCTGA AGCATCAGTC TACAAGTTTT 
GGTCTTGCCC TGACGTCGTA TATAGCTTCC TGCTGGAATC AAGACGGGGA TCCTGTGGAG 
CTTCTGAAGA TTGCAGATAA AGTGTCACAG TTCAGGCAAT GCCTTAAAGA AAATCCCATG 
TTTCTTCAGG AAAAAGTTAA AATGTATTTT AAGGATAATC CACATAGATT GACTCTGTCA 
ATGAGTCCAG AGGAAGACTA CTATGATAAA CAAGCAAAAC TGGAAGCAGA AAAACTGAAA 
AAGAAGGTTA ATGCACTTTC AGAAGAAGAG AAAACCCAAA TCTTTGAAAA AGGTTTGGAA 
TTAATTGATC TTCAAAGCAA ACCTCAGGAT ACCTCTTGTC TCCCAGCTCT AAAAGTGTCT 
GACATTGAGC CCAAAATCCC ATTCACTGTG CTGGAGACAA CATTTGCAGC TGATGAAGTT 
CCTGTCCAGT ATTGTTCTCA GCCAACCAAT GGGGTTGTGT ACTTCAGAGC TGTTTCCAGC 
TTGAACACTC TTCCTGAGGA GCTCAAACCA TACGTGCCGC TGTTTTGCAA CGTCATTACA 
AAGCTGGGAT GTGGAGCCCT TGATTACAGG GAACAAGCCC AGAGGATAGA GCTAAAAACA 
GGTGGGATGT CTGTCAGCCC ACATATCATT CCAGATGATT CACACCTGGA TGTGTATGAA 
CAGGGTGTGC TCTTTTCATC TCTGTGCTTG GATAGAAATC TGCCAGACAT GATGCATTTA 
TGGAGTGAAA TATTTAACAA CCCTCGATTT GAGGAGGAAG AGCACTTCAG AGTGCTGGTT 
AAGAAGACTG CCCAGGAACT ATCAAATGGG ATTCCAGATT GTGGACATTT ATATGCTTCT 
ATTAGAGCCA GCAAAAACCT TACGCCTTCT GGAGAACTGC AAGAAATGTT CAGTGGGATG 
GATCAGGTGA AGTTGATGAA GAGAATAGCA GAAATGCCTG ATATTAAACC AATACTACGC 
AAACTTCCAC GGATTAAGAA GTATCTGTTA AATAGTGACA ACATCCGATG TTCTGTGAAT 
GCAGCACCTC AACAGATGTC AGAGGCATCG AAAGAAATAG AGAAATTCTT AAAGGGCATA 
ACTAGAAGTA AAAAGGAGAG AAAACCTGTC CGTCCCCATG TGATTGAGAA ATCTTCAGAA 
GTCAGACCTG TGGGAAGCAA AATGCTTAGT AGTTTGCAGA TCACAAGGAA ACTAATTAAT 
GATCCTACAT TCAAACCATG CCCGATGAAG ACACACTTTG TACTTCCCTT CCCAGTGAAC 
TATATTGGGG AATGTGTTCG AACAGTACCG TACACAGCTA CAGACTATGC AAGCCTTCGA 
ATTCTGGCAA GGCTGATGAC ATCTAAGTTC CTACATAGAG AAATTAGAGA GAAAGGAGGG 
GCTTATGGTG GAGGTGCTAA ACTTAGTCAC AATGGAATAT TCACTTTCTA TTCCTACAGG 
GACCCAAATT CGTTAGCCAC CTTGAAAACA TTTGAAAAAG CAGTCGAGTG GGCCAAGTCT 
GGAGAATTCA CGCAAGAAGA TATTGATGAA GCTAAATTGG CAGTATTTGC TGCTGTAGAT 
GCACCAATAG CTCCTTCAGA TAAAGGTATG GACCATTTCT TATACGGGAT TTCAGATGAA 
ATGAAGCAGT CACACAGGGA GCAGCTTTTT GCTGTTAACA AAGATAACCT GGTTGATGTA 
GCAAATGAGT ATCTTGCAGT TGGGAAGAGC ACTCGTGGGC TGGCTGTACT TGGCCCAGAA 
AATACGGATA TCACCAAAGA TCCCTCATGG GTCAAACGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_418564.2
CDS	66..3167
Translation	MWSRYRAAAGLLSRGRSLNQKSWRWKSIPANERALLYRVGEQIHGFTVTQVTAVPELFLTAVKLSHDSTGARYLHVAREDSNNLFSLQFRTTPMDSTGVPHILEHTVLCGSQQYPCRDPFFKMLNRSLSTFMNAFTASDYTLYPFSTQNPKDFRNLLSVYLDAAFFPCLRQLDFWQEGWRLEHENPTDPQTPLIFKGVVFNEMKGAFTDNERVFAQHLQNKLLPDHTYGVVSGGHPLSIPDLTWEQLKQFHASHYHPSNSRFFTYGNFPLEHHLKQIHEEALVKFERIESKTDIPKQKIWEKPQEHHITCGLDSFASDPSKQTTVSVSYLLTDITDVFETFTLSLLSSLLVDGPNSPFYKALIESGVGTDFSPDVGFNGSTREAYFSVGLQGIAERDIETVKQIIARTVDEVIAKGFEEDRIEALLHKIEIQLKHQSTSFGLALTSYIASCWNQDGDPVELLKIADKVSQFRQCLKENPMFLQEKVKMYFKDNPHRLTLSMSPEEDYYDKQAKLEAEKLKKKVNALSEEEKTQIFEKGLELIDLQSKPQDTSCLPALKVSDIEPKIPFTVLETTFAADEVPVQYCSQPTNGVVYFRAVSSLNTLPEELKPYVPLFCNVITKLGCGALDYREQAQRIELKTGGMSVSPHIIPDDSHLDVYEQGVLFSSLCLDRNLPDMMHLWSEIFNNPRFEEEEHFRVLVKKTAQELSNGIPDCGHLYASIRASKNLTPSGELQEMFSGMDQVKLMKRIAEMPDIKPILRKLPRIKKYLLNSDNIRCSVNAAPQQMSEASKEIEKFLKGITRSKKERKPVRPHVIEKSSEVRPVGSKMLSSLQITRKLINDPTFKPCPMKTHFVLPFPVNYIGECVRTVPYTATDYASLRILARLMTSKFLHREIREKGGAYGGGAKLSHNGIFTFYSYRDPNSLATLKTFEKAVEWAKSGEFTQEDIDEAKLAVFAAVDAPIAPSDKGMDHFLYGISDEMKQSHREQLFAVNKDNLVDVANEYLAVGKSTRGLAVLGPENTDITKDPSWVKR

Target ORF information:

RefSeq Version	XM_418564.4
Organism	Gallus gallus(chicken)
Definition	Gallus gallus pitrilysin metallopeptidase 1 (PITRM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_418564.4

ORF Insert Sequence:

ATGTGGAGCC GCTACCGGGC GGCCGCCGGG CTGCTGAGCC GGGGAAGGTC TCTGAATCAG 
AAGTCATGGA GGTGGAAGAG CATTCCTGCC AACGAACGGG CGCTTCTTTA TAGGGTTGGA 
GAACAAATCC ATGGCTTCAC GGTAACTCAG GTTACAGCTG TTCCTGAGCT GTTCCTGACT 
GCAGTGAAAC TTAGTCATGA CAGTACAGGA GCCAGATATT TACACGTTGC TCGAGAGGAT 
TCCAATAATC TATTCAGTTT ACAATTCCGA ACCACTCCAA TGGACAGCAC TGGGGTCCCA 
CATATCCTTG AGCACACGGT GTTATGTGGT TCTCAGCAGT ATCCTTGCAG AGATCCGTTC 
TTTAAAATGT TAAACAGATC ATTGTCCACT TTCATGAATG CATTTACGGC TAGTGATTAC 
ACGCTCTATC CATTTTCAAC ACAAAATCCC AAGGATTTCC GGAATCTCCT GTCAGTGTAT 
TTGGATGCAG CTTTCTTTCC GTGTTTGCGG CAACTAGATT TCTGGCAAGA AGGTTGGAGG 
TTAGAACATG AGAACCCAAC TGATCCTCAG ACACCTCTAA TTTTCAAAGG AGTTGTTTTT 
AATGAAATGA AAGGGGCATT TACAGATAAC GAGAGGGTAT TTGCACAGCA CCTGCAGAAT 
AAACTCCTTC CTGATCACAC TTATGGTGTA GTATCTGGTG GACATCCCTT AAGTATTCCT 
GATCTTACAT GGGAGCAGCT TAAACAGTTC CATGCTTCAC ATTATCATCC CAGCAATTCC 
AGATTCTTCA CTTATGGTAA TTTTCCACTG GAGCATCACT TGAAACAAAT TCATGAAGAA 
GCACTGGTAA AATTTGAAAG AATTGAATCA AAGACTGACA TCCCAAAACA GAAAATCTGG 
GAAAAGCCTC AAGAACACCA TATAACATGT GGCTTAGACT CATTTGCTAG TGATCCTTCC 
AAGCAAACGA CTGTCAGCGT CAGTTACTTG TTGACAGACA TTACAGACGT TTTTGAAACT 
TTCACGCTAA GTCTCTTGTC TTCACTATTG GTTGATGGAC CAAATTCTCC ATTTTACAAA 
GCCTTAATTG AGTCTGGCGT TGGTACAGAT TTTTCTCCTG ATGTTGGGTT TAATGGCTCA 
ACAAGAGAAG CCTATTTCAG TGTGGGTCTG CAGGGTATTG CTGAGAGAGA CATTGAGACT 
GTGAAACAGA TTATTGCTAG AACAGTTGAT GAAGTTATTG CGAAAGGATT TGAAGAAGAT 
AGGATTGAAG CTCTACTTCA CAAAATTGAA ATCCAGCTGA AGCATCAGTC TACAAGTTTT 
GGTCTTGCCC TGACGTCGTA TATAGCTTCC TGCTGGAATC AAGACGGGGA TCCTGTGGAG 
CTTCTGAAGA TTGCAGATAA AGTGTCACAG TTCAGGCAAT GCCTTAAAGA AAATCCCATG 
TTTCTTCAGG AAAAAGTTAA AATGTATTTT AAGGATAATC CACATAGATT GACTCTGTCA 
ATGAGTCCAG AGGAAGACTA CTATGATAAA CAAGCAAAAC TGGAAGCAGA AAAACTGAAA 
AAGAAGGTTA ATGCACTTTC AGAAGAAGAG AAAACCCAAA TCTTTGAAAA AGGTTTGGAA 
TTAATTGATC TTCAAAGCAA ACCTCAGGAT ACCTCTTGTC TCCCAGCTCT AAAAGTGTCT 
GACATTGAGC CCAAAATCCC ATTCACTGTG CTGGAGACAA CATTTGCAGC TGATGAAGTT 
CCTGTCCAGT ATTGTTCTCA GCCAACCAAT GGGGTTGTGT ACTTCAGAGC TGTTTCCAGC 
TTGAACACTC TTCCTGAGGA GCTCAAACCA TACGTGCCGC TGTTTTGCAA CGTCATTACA 
AAGCTGGGAT GTGGAGCCCT TGATTACAGG GAACAAGCCC AGAGGATAGA GCTAAAAACA 
GGTGGGATGT CTGTCAGCCC ACATATCATT CCAGATGATT CACACCTGGA TGTGTATGAA 
CAGGGTGTGC TCTTTTCATC TCTGTGCTTG GATAGAAATC TGCCAGACAT GATGCATTTA 
TGGAGTGAAA TATTTAACAA CCCTCGATTT GAGGAGGAAG AGCACTTCAG AGTGCTGGTT 
AAGAAGACTG CCCAGGAACT ATCAAATGGG ATTCCAGATT GTGGACATTT ATATGCTTCT 
ATTAGAGCCA GCAAAAACCT TACGCCTTCT GGAGAACTGC AAGAAATGTT CAGTGGGATG 
GATCAGGTGA AGTTGATGAA GAGAATAGCA GAAATGCCTG ATATTAAACC AATACTACGC 
AAACTTCCAC GGATTAAGAA GTATCTGTTA AATAGTGACA ACATCCGATG TTCTGTGAAT 
GCAGCACCTC AACAGATGTC AGAGGCATCG AAAGAAATAG AGAAATTCTT AAAGGGCATA 
ACTAGAAGTA AAAAGGAGAG AAAACCTGTC CGTCCCCATG TGATTGAGAA ATCTTCAGAA 
GTCAGACCTG TGGGAAGCAA AATGCTTAGT AGTTTGCAGA TCACAAGGAA ACTAATTAAT 
GATCCTACAT TCAAACCATG CCCGATGAAG ACACACTTTG TACTTCCCTT CCCAGTGAAC 
TATATTGGGG AATGTGTTCG AACAGTACCG TACACAGCTA CAGACTATGC AAGCCTTCGA 
ATTCTGGCAA GGCTGATGAC ATCTAAGTTC CTACATAGAG AAATTAGAGA GAAAGGAGGG 
GCTTATGGTG GAGGTGCTAA ACTTAGTCAC AATGGAATAT TCACTTTCTA TTCCTACAGG 
GACCCAAATT CGTTAGCCAC CTTGAAAACA TTTGAAAAAG CAGTCGAGTG GGCCAAGTCT 
GGAGAATTCA CGCAAGAAGA TATTGATGAA GCTAAATTGG CAGTATTTGC TGCTGTAGAT 
GCACCAATAG CTCCTTCAGA TAAAGGTATG GACCATTTCT TATACGGGAT TTCAGATGAA 
ATGAAGCAGT CACACAGGGA GCAGCTTTTT GCTGTTAACA AAGATAACCT GGTTGATGTA 
GCAAATGAGT ATCTTGCAGT TGGGAAGAGC ACTCGTGGGC TGGCTGTACT TGGCCCAGAA 
AATACGGATA TCACCAAAGA TCCCTCATGG GTCAAACGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PITRM1 ( XM_418564.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_418564.2 Gallus gallus pitrilysin metallopeptidase 1 (PITRM1), mRNA.

Price & Availability↑

CloneID	OGa04027
Clone ID Related Accession (Same CDS sequence)	XM_418564.4 , XM_418564.5
Accession Version	XM_418564.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	presequence protease, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_418564.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAGCC GCTACCGGGC GGCCGCCGGG CTGCTGAGCC GGGGAAGGTC TCTGAATCAG 
AAGTCATGGA GGTGGAAGAG CATTCCTGCC AACGAACGGG CGCTTCTTTA TAGGGTTGGA 
GAACAAATCC ATGGCTTCAC GGTAACTCAG GTTACAGCTG TTCCTGAGCT GTTCCTGACT 
GCAGTGAAAC TTAGTCATGA CAGTACAGGA GCCAGATATT TACACGTTGC TCGAGAGGAT 
TCCAATAATC TATTCAGTTT ACAATTCCGA ACCACTCCAA TGGACAGCAC TGGGGTCCCA 
CATATCCTTG AGCACACGGT GTTATGTGGT TCTCAGCAGT ATCCTTGCAG AGATCCGTTC 
TTTAAAATGT TAAACAGATC ATTGTCCACT TTCATGAATG CATTTACGGC TAGTGATTAC 
ACGCTCTATC CATTTTCAAC ACAAAATCCC AAGGATTTCC GGAATCTCCT GTCAGTGTAT 
TTGGATGCAG CTTTCTTTCC GTGTTTGCGG CAACTAGATT TCTGGCAAGA AGGTTGGAGG 
TTAGAACATG AGAACCCAAC TGATCCTCAG ACACCTCTAA TTTTCAAAGG AGTTGTTTTT 
AATGAAATGA AAGGGGCATT TACAGATAAC GAGAGGGTAT TTGCACAGCA CCTGCAGAAT 
AAACTCCTTC CTGATCACAC TTATGGTGTA GTATCTGGTG GACATCCCTT AAGTATTCCT 
GATCTTACAT GGGAGCAGCT TAAACAGTTC CATGCTTCAC ATTATCATCC CAGCAATTCC 
AGATTCTTCA CTTATGGTAA TTTTCCACTG GAGCATCACT TGAAACAAAT TCATGAAGAA 
GCACTGGTAA AATTTGAAAG AATTGAATCA AAGACTGACA TCCCAAAACA GAAAATCTGG 
GAAAAGCCTC AAGAACACCA TATAACATGT GGCTTAGACT CATTTGCTAG TGATCCTTCC 
AAGCAAACGA CTGTCAGCGT CAGTTACTTG TTGACAGACA TTACAGACGT TTTTGAAACT 
TTCACGCTAA GTCTCTTGTC TTCACTATTG GTTGATGGAC CAAATTCTCC ATTTTACAAA 
GCCTTAATTG AGTCTGGCGT TGGTACAGAT TTTTCTCCTG ATGTTGGGTT TAATGGCTCA 
ACAAGAGAAG CCTATTTCAG TGTGGGTCTG CAGGGTATTG CTGAGAGAGA CATTGAGACT 
GTGAAACAGA TTATTGCTAG AACAGTTGAT GAAGTTATTG CGAAAGGATT TGAAGAAGAT 
AGGATTGAAG CTCTACTTCA CAAAATTGAA ATCCAGCTGA AGCATCAGTC TACAAGTTTT 
GGTCTTGCCC TGACGTCGTA TATAGCTTCC TGCTGGAATC AAGACGGGGA TCCTGTGGAG 
CTTCTGAAGA TTGCAGATAA AGTGTCACAG TTCAGGCAAT GCCTTAAAGA AAATCCCATG 
TTTCTTCAGG AAAAAGTTAA AATGTATTTT AAGGATAATC CACATAGATT GACTCTGTCA 
ATGAGTCCAG AGGAAGACTA CTATGATAAA CAAGCAAAAC TGGAAGCAGA AAAACTGAAA 
AAGAAGGTTA ATGCACTTTC AGAAGAAGAG AAAACCCAAA TCTTTGAAAA AGGTTTGGAA 
TTAATTGATC TTCAAAGCAA ACCTCAGGAT ACCTCTTGTC TCCCAGCTCT AAAAGTGTCT 
GACATTGAGC CCAAAATCCC ATTCACTGTG CTGGAGACAA CATTTGCAGC TGATGAAGTT 
CCTGTCCAGT ATTGTTCTCA GCCAACCAAT GGGGTTGTGT ACTTCAGAGC TGTTTCCAGC 
TTGAACACTC TTCCTGAGGA GCTCAAACCA TACGTGCCGC TGTTTTGCAA CGTCATTACA 
AAGCTGGGAT GTGGAGCCCT TGATTACAGG GAACAAGCCC AGAGGATAGA GCTAAAAACA 
GGTGGGATGT CTGTCAGCCC ACATATCATT CCAGATGATT CACACCTGGA TGTGTATGAA 
CAGGGTGTGC TCTTTTCATC TCTGTGCTTG GATAGAAATC TGCCAGACAT GATGCATTTA 
TGGAGTGAAA TATTTAACAA CCCTCGATTT GAGGAGGAAG AGCACTTCAG AGTGCTGGTT 
AAGAAGACTG CCCAGGAACT ATCAAATGGG ATTCCAGATT GTGGACATTT ATATGCTTCT 
ATTAGAGCCA GCAAAAACCT TACGCCTTCT GGAGAACTGC AAGAAATGTT CAGTGGGATG 
GATCAGGTGA AGTTGATGAA GAGAATAGCA GAAATGCCTG ATATTAAACC AATACTACGC 
AAACTTCCAC GGATTAAGAA GTATCTGTTA AATAGTGACA ACATCCGATG TTCTGTGAAT 
GCAGCACCTC AACAGATGTC AGAGGCATCG AAAGAAATAG AGAAATTCTT AAAGGGCATA 
ACTAGAAGTA AAAAGGAGAG AAAACCTGTC CGTCCCCATG TGATTGAGAA ATCTTCAGAA 
GTCAGACCTG TGGGAAGCAA AATGCTTAGT AGTTTGCAGA TCACAAGGAA ACTAATTAAT 
GATCCTACAT TCAAACCATG CCCGATGAAG ACACACTTTG TACTTCCCTT CCCAGTGAAC 
TATATTGGGG AATGTGTTCG AACAGTACCG TACACAGCTA CAGACTATGC AAGCCTTCGA 
ATTCTGGCAA GGCTGATGAC ATCTAAGTTC CTACATAGAG AAATTAGAGA GAAAGGAGGG 
GCTTATGGTG GAGGTGCTAA ACTTAGTCAC AATGGAATAT TCACTTTCTA TTCCTACAGG 
GACCCAAATT CGTTAGCCAC CTTGAAAACA TTTGAAAAAG CAGTCGAGTG GGCCAAGTCT 
GGAGAATTCA CGCAAGAAGA TATTGATGAA GCTAAATTGG CAGTATTTGC TGCTGTAGAT 
GCACCAATAG CTCCTTCAGA TAAAGGTATG GACCATTTCT TATACGGGAT TTCAGATGAA 
ATGAAGCAGT CACACAGGGA GCAGCTTTTT GCTGTTAACA AAGATAACCT GGTTGATGTA 
GCAAATGAGT ATCTTGCAGT TGGGAAGAGC ACTCGTGGGC TGGCTGTACT TGGCCCAGAA 
AATACGGATA TCACCAAAGA TCCCTCATGG GTCAAACGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_418564.2
CDS	16..3117
Translation	MWSRYRAAAGLLSRGRSLNQKSWRWKSIPANERALLYRVGEQIHGFTVTQVTAVPELFLTAVKLSHDSTGARYLHVAREDSNNLFSLQFRTTPMDSTGVPHILEHTVLCGSQQYPCRDPFFKMLNRSLSTFMNAFTASDYTLYPFSTQNPKDFRNLLSVYLDAAFFPCLRQLDFWQEGWRLEHENPTDPQTPLIFKGVVFNEMKGAFTDNERVFAQHLQNKLLPDHTYGVVSGGHPLSIPDLTWEQLKQFHASHYHPSNSRFFTYGNFPLEHHLKQIHEEALVKFERIESKTDIPKQKIWEKPQEHHITCGLDSFASDPSKQTTVSVSYLLTDITDVFETFTLSLLSSLLVDGPNSPFYKALIESGVGTDFSPDVGFNGSTREAYFSVGLQGIAERDIETVKQIIARTVDEVIAKGFEEDRIEALLHKIEIQLKHQSTSFGLALTSYIASCWNQDGDPVELLKIADKVSQFRQCLKENPMFLQEKVKMYFKDNPHRLTLSMSPEEDYYDKQAKLEAEKLKKKVNALSEEEKTQIFEKGLELIDLQSKPQDTSCLPALKVSDIEPKIPFTVLETTFAADEVPVQYCSQPTNGVVYFRAVSSLNTLPEELKPYVPLFCNVITKLGCGALDYREQAQRIELKTGGMSVSPHIIPDDSHLDVYEQGVLFSSLCLDRNLPDMMHLWSEIFNNPRFEEEEHFRVLVKKTAQELSNGIPDCGHLYASIRASKNLTPSGELQEMFSGMDQVKLMKRIAEMPDIKPILRKLPRIKKYLLNSDNIRCSVNAAPQQMSEASKEIEKFLKGITRSKKERKPVRPHVIEKSSEVRPVGSKMLSSLQITRKLINDPTFKPCPMKTHFVLPFPVNYIGECVRTVPYTATDYASLRILARLMTSKFLHREIREKGGAYGGGAKLSHNGIFTFYSYRDPNSLATLKTFEKAVEWAKSGEFTQEDIDEAKLAVFAAVDAPIAPSDKGMDHFLYGISDEMKQSHREQLFAVNKDNLVDVANEYLAVGKSTRGLAVLGPENTDITKDPSWVKR

Target ORF information:

RefSeq Version	XM_418564.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus pitrilysin metallopeptidase 1 (PITRM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_418564.5

ORF Insert Sequence:

ATGTGGAGCC GCTACCGGGC GGCCGCCGGG CTGCTGAGCC GGGGAAGGTC TCTGAATCAG 
AAGTCATGGA GGTGGAAGAG CATTCCTGCC AACGAACGGG CGCTTCTTTA TAGGGTTGGA 
GAACAAATCC ATGGCTTCAC GGTAACTCAG GTTACAGCTG TTCCTGAGCT GTTCCTGACT 
GCAGTGAAAC TTAGTCATGA CAGTACAGGA GCCAGATATT TACACGTTGC TCGAGAGGAT 
TCCAATAATC TATTCAGTTT ACAATTCCGA ACCACTCCAA TGGACAGCAC TGGGGTCCCA 
CATATCCTTG AGCACACGGT GTTATGTGGT TCTCAGCAGT ATCCTTGCAG AGATCCGTTC 
TTTAAAATGT TAAACAGATC ATTGTCCACT TTCATGAATG CATTTACGGC TAGTGATTAC 
ACGCTCTATC CATTTTCAAC ACAAAATCCC AAGGATTTCC GGAATCTCCT GTCAGTGTAT 
TTGGATGCAG CTTTCTTTCC GTGTTTGCGG CAACTAGATT TCTGGCAAGA AGGTTGGAGG 
TTAGAACATG AGAACCCAAC TGATCCTCAG ACACCTCTAA TTTTCAAAGG AGTTGTTTTT 
AATGAAATGA AAGGGGCATT TACAGATAAC GAGAGGGTAT TTGCACAGCA CCTGCAGAAT 
AAACTCCTTC CTGATCACAC TTATGGTGTA GTATCTGGTG GACATCCCTT AAGTATTCCT 
GATCTTACAT GGGAGCAGCT TAAACAGTTC CATGCTTCAC ATTATCATCC CAGCAATTCC 
AGATTCTTCA CTTATGGTAA TTTTCCACTG GAGCATCACT TGAAACAAAT TCATGAAGAA 
GCACTGGTAA AATTTGAAAG AATTGAATCA AAGACTGACA TCCCAAAACA GAAAATCTGG 
GAAAAGCCTC AAGAACACCA TATAACATGT GGCTTAGACT CATTTGCTAG TGATCCTTCC 
AAGCAAACGA CTGTCAGCGT CAGTTACTTG TTGACAGACA TTACAGACGT TTTTGAAACT 
TTCACGCTAA GTCTCTTGTC TTCACTATTG GTTGATGGAC CAAATTCTCC ATTTTACAAA 
GCCTTAATTG AGTCTGGCGT TGGTACAGAT TTTTCTCCTG ATGTTGGGTT TAATGGCTCA 
ACAAGAGAAG CCTATTTCAG TGTGGGTCTG CAGGGTATTG CTGAGAGAGA CATTGAGACT 
GTGAAACAGA TTATTGCTAG AACAGTTGAT GAAGTTATTG CGAAAGGATT TGAAGAAGAT 
AGGATTGAAG CTCTACTTCA CAAAATTGAA ATCCAGCTGA AGCATCAGTC TACAAGTTTT 
GGTCTTGCCC TGACGTCGTA TATAGCTTCC TGCTGGAATC AAGACGGGGA TCCTGTGGAG 
CTTCTGAAGA TTGCAGATAA AGTGTCACAG TTCAGGCAAT GCCTTAAAGA AAATCCCATG 
TTTCTTCAGG AAAAAGTTAA AATGTATTTT AAGGATAATC CACATAGATT GACTCTGTCA 
ATGAGTCCAG AGGAAGACTA CTATGATAAA CAAGCAAAAC TGGAAGCAGA AAAACTGAAA 
AAGAAGGTTA ATGCACTTTC AGAAGAAGAG AAAACCCAAA TCTTTGAAAA AGGTTTGGAA 
TTAATTGATC TTCAAAGCAA ACCTCAGGAT ACCTCTTGTC TCCCAGCTCT AAAAGTGTCT 
GACATTGAGC CCAAAATCCC ATTCACTGTG CTGGAGACAA CATTTGCAGC TGATGAAGTT 
CCTGTCCAGT ATTGTTCTCA GCCAACCAAT GGGGTTGTGT ACTTCAGAGC TGTTTCCAGC 
TTGAACACTC TTCCTGAGGA GCTCAAACCA TACGTGCCGC TGTTTTGCAA CGTCATTACA 
AAGCTGGGAT GTGGAGCCCT TGATTACAGG GAACAAGCCC AGAGGATAGA GCTAAAAACA 
GGTGGGATGT CTGTCAGCCC ACATATCATT CCAGATGATT CACACCTGGA TGTGTATGAA 
CAGGGTGTGC TCTTTTCATC TCTGTGCTTG GATAGAAATC TGCCAGACAT GATGCATTTA 
TGGAGTGAAA TATTTAACAA CCCTCGATTT GAGGAGGAAG AGCACTTCAG AGTGCTGGTT 
AAGAAGACTG CCCAGGAACT ATCAAATGGG ATTCCAGATT GTGGACATTT ATATGCTTCT 
ATTAGAGCCA GCAAAAACCT TACGCCTTCT GGAGAACTGC AAGAAATGTT CAGTGGGATG 
GATCAGGTGA AGTTGATGAA GAGAATAGCA GAAATGCCTG ATATTAAACC AATACTACGC 
AAACTTCCAC GGATTAAGAA GTATCTGTTA AATAGTGACA ACATCCGATG TTCTGTGAAT 
GCAGCACCTC AACAGATGTC AGAGGCATCG AAAGAAATAG AGAAATTCTT AAAGGGCATA 
ACTAGAAGTA AAAAGGAGAG AAAACCTGTC CGTCCCCATG TGATTGAGAA ATCTTCAGAA 
GTCAGACCTG TGGGAAGCAA AATGCTTAGT AGTTTGCAGA TCACAAGGAA ACTAATTAAT 
GATCCTACAT TCAAACCATG CCCGATGAAG ACACACTTTG TACTTCCCTT CCCAGTGAAC 
TATATTGGGG AATGTGTTCG AACAGTACCG TACACAGCTA CAGACTATGC AAGCCTTCGA 
ATTCTGGCAA GGCTGATGAC ATCTAAGTTC CTACATAGAG AAATTAGAGA GAAAGGAGGG 
GCTTATGGTG GAGGTGCTAA ACTTAGTCAC AATGGAATAT TCACTTTCTA TTCCTACAGG 
GACCCAAATT CGTTAGCCAC CTTGAAAACA TTTGAAAAAG CAGTCGAGTG GGCCAAGTCT 
GGAGAATTCA CGCAAGAAGA TATTGATGAA GCTAAATTGG CAGTATTTGC TGCTGTAGAT 
GCACCAATAG CTCCTTCAGA TAAAGGTATG GACCATTTCT TATACGGGAT TTCAGATGAA 
ATGAAGCAGT CACACAGGGA GCAGCTTTTT GCTGTTAACA AAGATAACCT GGTTGATGTA 
GCAAATGAGT ATCTTGCAGT TGGGAAGAGC ACTCGTGGGC TGGCTGTACT TGGCCCAGAA 
AATACGGATA TCACCAAAGA TCCCTCATGG GTCAAACGAT GA