UBE4B cDNA ORF clone, Gallus gallus(chicken)

The following UBE4B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE4B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa147063 XM_025142568.1
Latest version!
Gallus gallus ubiquitination factor E4B (UBE4B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa18251 XM_004947452.3
Latest version!
Gallus gallus ubiquitination factor E4B (UBE4B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa18252 XM_004947453.3
Latest version!
Gallus gallus ubiquitination factor E4B (UBE4B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa18254 XM_003642511.4
Latest version!
Gallus gallus ubiquitination factor E4B (UBE4B), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa45208 XM_015297149.2
Latest version!
Gallus gallus ubiquitination factor E4B (UBE4B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa45209 XM_015297150.2
Latest version!
Gallus gallus ubiquitination factor E4B (UBE4B), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
View Old Accession Versions >>
OGa45209 XM_015297150.1 Gallus gallus ubiquitination factor E4B (UBE4B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa18254 XM_003642511.3 Gallus gallus ubiquitination factor E4B (UBE4B), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa18251 XM_004947452.2 Gallus gallus ubiquitination factor E4B (UBE4B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa18252 XM_004947453.2 Gallus gallus ubiquitination factor E4B (UBE4B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa45208 XM_015297149.1 Gallus gallus ubiquitination factor E4B (UBE4B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa147063
    Clone ID Related Accession (Same CDS sequence) XM_025142568.1
    Accession Version XM_025142568.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3639bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ubiquitin conjugation factor E4 B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006108.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAGGAGC TGAGCGCGGA CGAGATCCGT CGAAGGCGCC TTGCCCGGCT GGCAGGGGGA 
    CCATCGTCCC AGCCCAGCAC ACCGCTCACA TCGCCGCAGA GAGAGACCCC ACCTGGGCCA
    CCTGCAGCAG CTCCGACCCC GGGGCCATCC CATAGCCTCG GCCTAAATGT CCACAGCATG
    ACCCCAGCTA CCTCTCCGAT TGGTGCATCA GGAGTAGCCC ACCGAAGCCA GAGCAGCGAA
    GGGGTGAGTT CGCTCAGCAG TTCTCCATCA AACAGCCTTG AAACACAATC CCAGTCTCTC
    TCTCGGTCTC AGAGTATGGA TATTGATAGT GTCTCCTGTG AAAAAAGTAT GTCCCAGGTA
    GATGTGGATT CAGGAATTGA GAACATGGAA GTGGATGAGA ATGATCGCAG GGAGAAAAGG
    AGTCTGACTG ATAAGGAGGA GCCTCTGTCT GGGTCTGAGG TGTCCGAGGA GCAAGCTTTA
    CAGCTGGTCT GTAAAATCTT CAGAGTCTCT TGGAAGGACC GAGACAGAGA TGTTATCTTC
    CTGAATTCTC TCTCTGCTCA GTTTAAGCAG AACCCAAAAG AAGTGTTTTC GGATTTTAAG
    GACTTGATCG GACAGATCTT GATGGAGGTG CTGATGATGT CCACCCAGTC AAGGGATGAA
    AACCCATTTG CCAGTCTGAC AGCTACGTCA CAGCCAATTG CTGCAACTGC CCGGTCTCCT
    GACAGAAGCC TGATCTTAAA CACGGGCTCC AACCCAGGAA CCAGCCCTAT GTTCTGTAAT
    GTGGGCTCCT TTGGTTCCAG TTCGTTATCC AGTATATACG ACAACCCTTT CTCCCTCCTC
    TTCCTTGGCG TTTCTGATGT GTCTGAGGAC AGTAGTGACG AAGAAAATGA AGATGATGAT
    TTTTCTTGTG TCCAGTTTGG GTCCAGTGTG GGAGGCTCTG GCAGCTCAAG TGTTTGCGAT
    TCCTGCAGTG ACCACTTCAC CATTGAAAAC TGTAAGGAGA CAGAGATGCT GAACTACCTC
    ATTGAGTGTT TTGACCGGGT TGGAATAGAG GAGCGAAAAG CACCAAAGAT GTGTAGCCAG
    CCAACAGTGA GTCAGTTACT GAGCAACATA CGATCGCAGT GCATTTCACA TGCTGCTTTG
    GTGCTACAGG GATCCTTAAC GCAACCAAGG TCATTGCAGC AGCAGTCTTT GCTGGTACCA
    TATATGCTGT GTAGGAATCT CCCATTTGGC TTCATCCAAG AACTGGTGAG AACAACTTAC
    CAAGATGAAG AGGTGTTCAA ACAGATCTTT ATCCCCATCC TGCAAGGCCT GGCTGTAGCT
    TCCAAAGAGT GCTCCCTTGA TAGTGACAAT TTTAAGTACC CCCTTATGGC ATTATGTGAA
    CTTTGTGAAA TCAAGTTTGG AAAAACACAC CCCATGTGCA GTTTGGTTGT CTCTTTGCCC
    TTGTGGCTGC CTAAATCTTT AAGCACGGGT GCAGGAAGAG AGCTACAGAG ACTTTCCTAT
    CTTGGAGCCT TCTTCAGTCT CTCTGTCTTT GCTGAAGATG ACAACAAAGT TGTTGAAAAG
    TACTTCTCAG GACCTGCCAT CACTCTTGAA AACACCCGAG TTGTTAGCCA GTCCTTGCAG
    CATTACCTGG AACTAGCGAG GCAAGAGCTC TTCAAGATCC TGCATAGTAT TTTACTGAAT
    GGTGAAACTC GAGAGGCAGC TCTCAGTTAC ATGGCAGCTG TTGTCAACGC CAACATGAAA
    AAGGCACAAA TGCAGACAGA TGATCGTTTG GTGTCTACAG ATGGTTTTAT GCTCAACTTC
    CTATGGGTGC TGCAGCAACT AAGTATGAAG ATCAAGCTGG AAACGGTTGA TCCCATGTAC
    ATATTTCATC CCAGGTGTCG TATTGACCTC CCGACAGATG AGACAAGAGT GAAAGCAACA
    ATGGAGGATG TTACAGCCTG GATTGCTGAG CTTTACAGGG ATCCGTCTCC ATTTTCTGAT
    CCAAAATTCC CAACAGAATG TTTCTTCTTA ACCCTGCATG CCCATCATCT CTCCATCCTG
    CCGAGCTGCC GTCGGTACAT ACGTAGACTG CGAGCCATCA GGGAACTCAA CAGGACTGTT
    GAAGATCTGA AAAACAATGA AAGTCAATGG AAGGATTCTC CTCTGGCTAC CAGACACAGA
    GAGATGCTAA AACGTTGCAA GACACAACTT AAGAAACTGG TGAGGTGCAA GGCTTGTGCA
    GATGCTGGTT TGCTGGATGA GAACTTTCTC AGGAGATGTC TGAATTTCTA TGGCATGGTG
    ATTCAACTGA TGCTTCGCAT CCTCGACCCC GCCTATCCCA ACATAAAATT GCCTTTAACA
    CTTGAAGTTC CGAAGGTATT TGCAGCATTG CCAGAGTTTT ACGTGGAAGA TGTTGCTGAA
    TTTTTATTTT TTATTGTACA ATATGCTCCT CAGGTGCTTT ATGAGCCCTG TACTCAGGAC
    ATAGTGATGT TCCTTGTTGT GATGCTGTGC AACCAGAACT ACATCCGAAA TCCTTATTTA
    GTTGCTAAGC TGGTGGAGGT GATGTTCATG ACCAATCCAG CTGTTCAGCC TCGAACACAG
    AAGTTCTTTG AAATGATTGA GAATCATCCT CTGTCCACTA AATTGTTAGT CCCCTCCTTA
    ATGAAATTTT ACACAGATGT TGAGCATACT GGAGCAACTA GTGAATTCTA TGACAAGTTT
    ACAATCCGCT ACCACATCAG CACCATTTTC AAAAGCCTCT GGCAGAACAT AGCTCACCAT
    GGTACATTTA TGGAAGAGTT CAACTCTGGG AAGCAGTTTG TTCGCTACAT CAACATGCTG
    ATAAATGACA CAACTTTCTT GCTGGATGAG AGTCTGGAAT CCCTAAAACG TATCCATGAA
    GTGCAAGAGG AGATGAAGAA TAAAGAACAA TGGGACCTGC TGCCTAGGGA TCAGCAGCAG
    GCTCGGCAGT CGCAGCTGGC TCAGGATGAG CGTGTCTCTC GTTCATATCT TGCCCTGGCA
    ACAGAAACTG TTGATATGTT CCATATCCTT ACCAAGCAGG TTCAAAAGCC TTTTCTCAGA
    CCTGAACTTG GACCACGATT GGCTGCTATG TTGAACTTTA ACTTGCAGCA ACTATGTGGC
    CCGAAGTGCC GTGACCTAAA AGTTGAGAAC CCTGAGAAAT ACGGGTTTGA ACCAAAGAAG
    CTGTTGGACC AGCTGACTGA CATTTATCTA CAGCTAGACT GTGCTCGTTT TGCTAAAGCA
    ATTGCTGATG ATCAGAGGTC CTACAGCAAA GAGCTCTTTG AGGAGGTCAT ATCAAAGATG
    AGAAAGGCTG GAATCAAGTC AACCATAGCA ATAGAAAAAT TCAAACTGCT GGCAGAGAAA
    GTGGAAGAGA TTGTTGCCAA GAATGCCCGT GCAGAAATTG ATTACAGCGA TGCTCCGGAT
    GAATTCAGAG ATCCACTCAT GGACACTCTG ATGACTGATC CTGTGAGGTT GCCATCTGGA
    ACTATTATGG ACAGGTCAAT CATCCTGAGG CATCTGTTGA ATTCTTCCAC TGATCCCTTC
    AATCGTCAGA CACTGACAGA AAATATGCTG GAACCAGTGC CAGAACTTAA AGAGCAAATC
    CAGGCCTGGA TGAGAGACAA GCAGAATGCT GACCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024998336.1
    CDS117..3755
    Translation

    Target ORF information:

    RefSeq Version XM_025142568.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ubiquitination factor E4B (UBE4B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025142568.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAGGAGC TGAGCGCGGA CGAGATCCGT CGAAGGCGCC TTGCCCGGCT GGCAGGGGGA 
    CCATCGTCCC AGCCCAGCAC ACCGCTCACA TCGCCGCAGA GAGAGACCCC ACCTGGGCCA
    CCTGCAGCAG CTCCGACCCC GGGGCCATCC CATAGCCTCG GCCTAAATGT CCACAGCATG
    ACCCCAGCTA CCTCTCCGAT TGGTGCATCA GGAGTAGCCC ACCGAAGCCA GAGCAGCGAA
    GGGGTGAGTT CGCTCAGCAG TTCTCCATCA AACAGCCTTG AAACACAATC CCAGTCTCTC
    TCTCGGTCTC AGAGTATGGA TATTGATAGT GTCTCCTGTG AAAAAAGTAT GTCCCAGGTA
    GATGTGGATT CAGGAATTGA GAACATGGAA GTGGATGAGA ATGATCGCAG GGAGAAAAGG
    AGTCTGACTG ATAAGGAGGA GCCTCTGTCT GGGTCTGAGG TGTCCGAGGA GCAAGCTTTA
    CAGCTGGTCT GTAAAATCTT CAGAGTCTCT TGGAAGGACC GAGACAGAGA TGTTATCTTC
    CTGAATTCTC TCTCTGCTCA GTTTAAGCAG AACCCAAAAG AAGTGTTTTC GGATTTTAAG
    GACTTGATCG GACAGATCTT GATGGAGGTG CTGATGATGT CCACCCAGTC AAGGGATGAA
    AACCCATTTG CCAGTCTGAC AGCTACGTCA CAGCCAATTG CTGCAACTGC CCGGTCTCCT
    GACAGAAGCC TGATCTTAAA CACGGGCTCC AACCCAGGAA CCAGCCCTAT GTTCTGTAAT
    GTGGGCTCCT TTGGTTCCAG TTCGTTATCC AGTATATACG ACAACCCTTT CTCCCTCCTC
    TTCCTTGGCG TTTCTGATGT GTCTGAGGAC AGTAGTGACG AAGAAAATGA AGATGATGAT
    TTTTCTTGTG TCCAGTTTGG GTCCAGTGTG GGAGGCTCTG GCAGCTCAAG TGTTTGCGAT
    TCCTGCAGTG ACCACTTCAC CATTGAAAAC TGTAAGGAGA CAGAGATGCT GAACTACCTC
    ATTGAGTGTT TTGACCGGGT TGGAATAGAG GAGCGAAAAG CACCAAAGAT GTGTAGCCAG
    CCAACAGTGA GTCAGTTACT GAGCAACATA CGATCGCAGT GCATTTCACA TGCTGCTTTG
    GTGCTACAGG GATCCTTAAC GCAACCAAGG TCATTGCAGC AGCAGTCTTT GCTGGTACCA
    TATATGCTGT GTAGGAATCT CCCATTTGGC TTCATCCAAG AACTGGTGAG AACAACTTAC
    CAAGATGAAG AGGTGTTCAA ACAGATCTTT ATCCCCATCC TGCAAGGCCT GGCTGTAGCT
    TCCAAAGAGT GCTCCCTTGA TAGTGACAAT TTTAAGTACC CCCTTATGGC ATTATGTGAA
    CTTTGTGAAA TCAAGTTTGG AAAAACACAC CCCATGTGCA GTTTGGTTGT CTCTTTGCCC
    TTGTGGCTGC CTAAATCTTT AAGCACGGGT GCAGGAAGAG AGCTACAGAG ACTTTCCTAT
    CTTGGAGCCT TCTTCAGTCT CTCTGTCTTT GCTGAAGATG ACAACAAAGT TGTTGAAAAG
    TACTTCTCAG GACCTGCCAT CACTCTTGAA AACACCCGAG TTGTTAGCCA GTCCTTGCAG
    CATTACCTGG AACTAGCGAG GCAAGAGCTC TTCAAGATCC TGCATAGTAT TTTACTGAAT
    GGTGAAACTC GAGAGGCAGC TCTCAGTTAC ATGGCAGCTG TTGTCAACGC CAACATGAAA
    AAGGCACAAA TGCAGACAGA TGATCGTTTG GTGTCTACAG ATGGTTTTAT GCTCAACTTC
    CTATGGGTGC TGCAGCAACT AAGTATGAAG ATCAAGCTGG AAACGGTTGA TCCCATGTAC
    ATATTTCATC CCAGGTGTCG TATTGACCTC CCGACAGATG AGACAAGAGT GAAAGCAACA
    ATGGAGGATG TTACAGCCTG GATTGCTGAG CTTTACAGGG ATCCGTCTCC ATTTTCTGAT
    CCAAAATTCC CAACAGAATG TTTCTTCTTA ACCCTGCATG CCCATCATCT CTCCATCCTG
    CCGAGCTGCC GTCGGTACAT ACGTAGACTG CGAGCCATCA GGGAACTCAA CAGGACTGTT
    GAAGATCTGA AAAACAATGA AAGTCAATGG AAGGATTCTC CTCTGGCTAC CAGACACAGA
    GAGATGCTAA AACGTTGCAA GACACAACTT AAGAAACTGG TGAGGTGCAA GGCTTGTGCA
    GATGCTGGTT TGCTGGATGA GAACTTTCTC AGGAGATGTC TGAATTTCTA TGGCATGGTG
    ATTCAACTGA TGCTTCGCAT CCTCGACCCC GCCTATCCCA ACATAAAATT GCCTTTAACA
    CTTGAAGTTC CGAAGGTATT TGCAGCATTG CCAGAGTTTT ACGTGGAAGA TGTTGCTGAA
    TTTTTATTTT TTATTGTACA ATATGCTCCT CAGGTGCTTT ATGAGCCCTG TACTCAGGAC
    ATAGTGATGT TCCTTGTTGT GATGCTGTGC AACCAGAACT ACATCCGAAA TCCTTATTTA
    GTTGCTAAGC TGGTGGAGGT GATGTTCATG ACCAATCCAG CTGTTCAGCC TCGAACACAG
    AAGTTCTTTG AAATGATTGA GAATCATCCT CTGTCCACTA AATTGTTAGT CCCCTCCTTA
    ATGAAATTTT ACACAGATGT TGAGCATACT GGAGCAACTA GTGAATTCTA TGACAAGTTT
    ACAATCCGCT ACCACATCAG CACCATTTTC AAAAGCCTCT GGCAGAACAT AGCTCACCAT
    GGTACATTTA TGGAAGAGTT CAACTCTGGG AAGCAGTTTG TTCGCTACAT CAACATGCTG
    ATAAATGACA CAACTTTCTT GCTGGATGAG AGTCTGGAAT CCCTAAAACG TATCCATGAA
    GTGCAAGAGG AGATGAAGAA TAAAGAACAA TGGGACCTGC TGCCTAGGGA TCAGCAGCAG
    GCTCGGCAGT CGCAGCTGGC TCAGGATGAG CGTGTCTCTC GTTCATATCT TGCCCTGGCA
    ACAGAAACTG TTGATATGTT CCATATCCTT ACCAAGCAGG TTCAAAAGCC TTTTCTCAGA
    CCTGAACTTG GACCACGATT GGCTGCTATG TTGAACTTTA ACTTGCAGCA ACTATGTGGC
    CCGAAGTGCC GTGACCTAAA AGTTGAGAAC CCTGAGAAAT ACGGGTTTGA ACCAAAGAAG
    CTGTTGGACC AGCTGACTGA CATTTATCTA CAGCTAGACT GTGCTCGTTT TGCTAAAGCA
    ATTGCTGATG ATCAGAGGTC CTACAGCAAA GAGCTCTTTG AGGAGGTCAT ATCAAAGATG
    AGAAAGGCTG GAATCAAGTC AACCATAGCA ATAGAAAAAT TCAAACTGCT GGCAGAGAAA
    GTGGAAGAGA TTGTTGCCAA GAATGCCCGT GCAGAAATTG ATTACAGCGA TGCTCCGGAT
    GAATTCAGAG ATCCACTCAT GGACACTCTG ATGACTGATC CTGTGAGGTT GCCATCTGGA
    ACTATTATGG ACAGGTCAAT CATCCTGAGG CATCTGTTGA ATTCTTCCAC TGATCCCTTC
    AATCGTCAGA CACTGACAGA AAATATGCTG GAACCAGTGC CAGAACTTAA AGAGCAAATC
    CAGGCCTGGA TGAGAGACAA GCAGAATGCT GACCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa18251
    Clone ID Related Accession (Same CDS sequence) XM_004947452.3 , XM_004947452.2
    Accession Version XM_004947452.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4044bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ubiquitin conjugation factor E4 B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006108.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004947452.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGAGC TGAGCGCGGA CGAGATCCGT CGAAGGCGCC TTGCCCGGCT GGCAGGGGGA 
    CCATCGTCCC AGCCCAGCAC ACCGCTCACA TCGCCGCAGA GAGAGACCCC ACCTGGGCCA
    CCTGCAGCAG CTCCGACCCC GGGGCCATCC CATAGCCTCG GCCTAAATGT CCACAGCATG
    ACCCCAGCTA CCTCTCCGAT TGGTGCATCA GGAGTAGCCC ACCGAAGCCA GAGCAGCGAA
    GGGGTGAGTT CGCTCAGCAG TTCTCCATCA AACAGCCTTG AAACACAATC CCAGTCTCTC
    TCTCGGTCTC AGAGTATGGA TATTGATAGT GTCTCCTGTG AAAAAAGTAT GTCCCAGGTA
    GATGTGGATT CAGGAATTGA GAACATGGAA GTGGATGAGA ATGATCGCAG GGAGAAAAGG
    AGTCTGACTG ATAAGGAGGA GCCTCTGTCT GGGTCTGAGG TGTCCGAGGA GCAAGCTTTA
    CAGCTGGTCT GTAAAATCTT CAGAGTCTCT TGGAAGGACC GAGACAGAGA TGTTATCTTC
    CTGAATTCTC TCTCTGCTCA GTTTAAGCAG AACCCAAAAG AAGTGTTTTC GGATTTTAAG
    GACTTGATCG GACAGATCTT GATGGAGGTG CTGATGATGT CCACCCAGTC AAGGGATGAA
    AACCCATTTG CCAGTCTGAC AGCTACGTCA CAGCCAATTG CTGCAACTGC CCGGTCTCCT
    GACAGAAGCC TGATCTTAAA CACGGGCTCC AACCCAGGAA CCAGCCCTAT GTTCTGTAAT
    GTGGGCTCCT TTGGTTCCAG TTCGTTATCC AGTCTCTATG GGACTAGTCC AGCTCCTACT
    ACCAATTTCA CAAGCTATGT ACCAATGACT GGTTCATCTC TTACCCCACC AGCCAGTTCA
    GTTGCCACCA CATCTGTGCC TTCCATTACT GTCAGCTCTC ACCTCACAGC AACCAGCCCT
    TCTTGGACCC CGCCTTCCTC TCCGAGGTAC CGGCCATATA CTGTTGCCCA TTCTGGAGGC
    TTTTCAGCTT CTTCTATCCC ACGTTCCTTG AATATTTCCA TCCCAGCTAG TTCAAGTCCT
    CCAGTCATGG CTGCTAGCCC TCCAGCCATG CTGCTCACTG CACCCAGACA GAGACCCACG
    ACTATGGGTC CGCCTTTGTT TACTTCACCC AGTACCTTGC GCAGGCGTTC ATCTTTACTG
    AACAGGATTC CTTCTAGTAT ATACGACAAC CCTTTCTCCC TCCTCTTCCT TGGCGTTTCT
    GATGTGTCTG AGGACAGTAG TGACGAAGAA AATGAAGATG ATGATTTTTC TTGTGTCCAG
    TTTGGGTCCA GTGTGGGAGG CTCTGGCAGC TCAAGTGTTT GCGATTCCTG CAGTGACCAC
    TTCACCATTG AAAACTGTAA GGAGACAGAG ATGCTGAACT ACCTCATTGA GTGTTTTGAC
    CGGGTTGGAA TAGAGGAGCG AAAAGCACCA AAGATGTGTA GCCAGCCAAC AGTGAGTCAG
    TTACTGAGCA ACATACGATC GCAGTGCATT TCACATGCTG CTTTGGTGCT ACAGGGATCC
    TTAACGCAAC CAAGGTCATT GCAGCAGCAG TCTTTGCTGG TACCATATAT GCTGTGTAGG
    AATCTCCCAT TTGGCTTCAT CCAAGAACTG GTGAGAACAA CTTACCAAGA TGAAGAGGTG
    TTCAAACAGA TCTTTATCCC CATCCTGCAA GGCCTGGCTG TAGCTTCCAA AGAGTGCTCC
    CTTGATAGTG ACAATTTTAA GTACCCCCTT ATGGCATTAT GTGAACTTTG TGAAATCAAG
    TTTGGAAAAA CACACCCCAT GTGCAGTTTG GTTGTCTCTT TGCCCTTGTG GCTGCCTAAA
    TCTTTAAGCA CGGGTGCAGG AAGAGAGCTA CAGAGACTTT CCTATCTTGG AGCCTTCTTC
    AGTCTCTCTG TCTTTGCTGA AGATGACAAC AAAGTTGTTG AAAAGTACTT CTCAGGACCT
    GCCATCACTC TTGAAAACAC CCGAGTTGTT AGCCAGTCCT TGCAGCATTA CCTGGAACTA
    GCGAGGCAAG AGCTCTTCAA GATCCTGCAT AGTATTTTAC TGAATGGTGA AACTCGAGAG
    GCAGCTCTCA GTTACATGGC AGCTGTTGTC AACGCCAACA TGAAAAAGGC ACAAATGCAG
    ACAGATGATC GTTTGGTGTC TACAGATGGT TTTATGCTCA ACTTCCTATG GGTGCTGCAG
    CAACTAAGTA TGAAGATCAA GCTGGAAACG GTTGATCCCA TGTACATATT TCATCCCAGG
    TGTCGTATTG ACCTCCCGAC AGATGAGACA AGAGTGAAAG CAACAATGGA GGATGTTACA
    GCCTGGATTG CTGAGCTTTA CAGGGATCCG TCTCCATTTT CTGATCCAAA ATTCCCAACA
    GAATGTTTCT TCTTAACCCT GCATGCCCAT CATCTCTCCA TCCTGCCGAG CTGCCGTCGG
    TACATACGTA GACTGCGAGC CATCAGGGAA CTCAACAGGA CTGTTGAAGA TCTGAAAAAC
    AATGAAAGTC AATGGAAGGA TTCTCCTCTG GCTACCAGAC ACAGAGAGAT GCTAAAACGT
    TGCAAGACAC AACTTAAGAA ACTGGTGAGG TGCAAGGCTT GTGCAGATGC TGGTTTGCTG
    GATGAGAACT TTCTCAGGAG ATGTCTGAAT TTCTATGGCA TGGTGATTCA ACTGATGCTT
    CGCATCCTCG ACCCCGCCTA TCCCAACATA AAATTGCCTT TAACACTTGA AGTTCCGAAG
    GTATTTGCAG CATTGCCAGA GTTTTACGTG GAAGATGTTG CTGAATTTTT ATTTTTTATT
    GTACAATATG CTCCTCAGGT GCTTTATGAG CCCTGTACTC AGGACATAGT GATGTTCCTT
    GTTGTGATGC TGTGCAACCA GAACTACATC CGAAATCCTT ATTTAGTTGC TAAGCTGGTG
    GAGGTGATGT TCATGACCAA TCCAGCTGTT CAGCCTCGAA CACAGAAGTT CTTTGAAATG
    ATTGAGAATC ATCCTCTGTC CACTAAATTG TTAGTCCCCT CCTTAATGAA ATTTTACACA
    GATGTTGAGC ATACTGGAGC AACTAGTGAA TTCTATGACA AGTTTACAAT CCGCTACCAC
    ATCAGCACCA TTTTCAAAAG CCTCTGGCAG AACATAGCTC ACCATGGTAC ATTTATGGAA
    GAGTTCAACT CTGGGAAGCA GTTTGTTCGC TACATCAACA TGCTGATAAA TGACACAACT
    TTCTTGCTGG ATGAGAGTCT GGAATCCCTA AAACGTATCC ATGAAGTGCA AGAGGAGATG
    AAGAATAAAG AACAATGGGA CCTGCTGCCT AGGGATCAGC AGCAGGCTCG GCAGTCGCAG
    CTGGCTCAGG ATGAGCGTGT CTCTCGTTCA TATCTTGCCC TGGCAACAGA AACTGTTGAT
    ATGTTCCATA TCCTTACCAA GCAGGTTCAA AAGCCTTTTC TCAGACCTGA ACTTGGACCA
    CGATTGGCTG CTATGTTGAA CTTTAACTTG CAGCAACTAT GTGGCCCGAA GTGCCGTGAC
    CTAAAAGTTG AGAACCCTGA GAAATACGGG TTTGAACCAA AGAAGCTGTT GGACCAGCTG
    ACTGACATTT ATCTACAGCT AGACTGTGCT CGTTTTGCTA AAGCAATTGC TGATGATCAG
    AGGTCCTACA GCAAAGAGCT CTTTGAGGAG GTCATATCAA AGATGAGAAA GGCTGGAATC
    AAGTCAACCA TAGCAATAGA AAAATTCAAA CTGCTGGCAG AGAAAGTGGA AGAGATTGTT
    GCCAAGAATG CCCGTGCAGA AATTGATTAC AGCGATGCTC CGGATGAATT CAGAGATCCA
    CTCATGGACA CTCTGATGAC TGATCCTGTG AGGTTGCCAT CTGGAACTAT TATGGACAGG
    TCAATCATCC TGAGGCATCT GTTGAATTCT TCCACTGATC CCTTCAATCG TCAGACACTG
    ACAGAAAATA TGCTGGAACC AGTGCCAGAA CTTAAAGAGC AAATCCAGGC CTGGATGAGA
    GACAAGCAGA ATGCTGACCA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004947509.1
    CDS117..4160
    Translation

    Target ORF information:

    RefSeq Version XM_004947452.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ubiquitination factor E4B (UBE4B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004947452.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGAGC TGAGCGCGGA CGAGATCCGT CGAAGGCGCC TTGCCCGGCT GGCAGGGGGA 
    CCATCGTCCC AGCCCAGCAC ACCGCTCACA TCGCCGCAGA GAGAGACCCC ACCTGGGCCA
    CCTGCAGCAG CTCCGACCCC GGGGCCATCC CATAGCCTCG GCCTAAATGT CCACAGCATG
    ACCCCAGCTA CCTCTCCGAT TGGTGCATCA GGAGTAGCCC ACCGAAGCCA GAGCAGCGAA
    GGGGTGAGTT CGCTCAGCAG TTCTCCATCA AACAGCCTTG AAACACAATC CCAGTCTCTC
    TCTCGGTCTC AGAGTATGGA TATTGATAGT GTCTCCTGTG AAAAAAGTAT GTCCCAGGTA
    GATGTGGATT CAGGAATTGA GAACATGGAA GTGGATGAGA ATGATCGCAG GGAGAAAAGG
    AGTCTGACTG ATAAGGAGGA GCCTCTGTCT GGGTCTGAGG TGTCCGAGGA GCAAGCTTTA
    CAGCTGGTCT GTAAAATCTT CAGAGTCTCT TGGAAGGACC GAGACAGAGA TGTTATCTTC
    CTGAATTCTC TCTCTGCTCA GTTTAAGCAG AACCCAAAAG AAGTGTTTTC GGATTTTAAG
    GACTTGATCG GACAGATCTT GATGGAGGTG CTGATGATGT CCACCCAGTC AAGGGATGAA
    AACCCATTTG CCAGTCTGAC AGCTACGTCA CAGCCAATTG CTGCAACTGC CCGGTCTCCT
    GACAGAAGCC TGATCTTAAA CACGGGCTCC AACCCAGGAA CCAGCCCTAT GTTCTGTAAT
    GTGGGCTCCT TTGGTTCCAG TTCGTTATCC AGTCTCTATG GGACTAGTCC AGCTCCTACT
    ACCAATTTCA CAAGCTATGT ACCAATGACT GGTTCATCTC TTACCCCACC AGCCAGTTCA
    GTTGCCACCA CATCTGTGCC TTCCATTACT GTCAGCTCTC ACCTCACAGC AACCAGCCCT
    TCTTGGACCC CGCCTTCCTC TCCGAGGTAC CGGCCATATA CTGTTGCCCA TTCTGGAGGC
    TTTTCAGCTT CTTCTATCCC ACGTTCCTTG AATATTTCCA TCCCAGCTAG TTCAAGTCCT
    CCAGTCATGG CTGCTAGCCC TCCAGCCATG CTGCTCACTG CACCCAGACA GAGACCCACG
    ACTATGGGTC CGCCTTTGTT TACTTCACCC AGTACCTTGC GCAGGCGTTC ATCTTTACTG
    AACAGGATTC CTTCTAGTAT ATACGACAAC CCTTTCTCCC TCCTCTTCCT TGGCGTTTCT
    GATGTGTCTG AGGACAGTAG TGACGAAGAA AATGAAGATG ATGATTTTTC TTGTGTCCAG
    TTTGGGTCCA GTGTGGGAGG CTCTGGCAGC TCAAGTGTTT GCGATTCCTG CAGTGACCAC
    TTCACCATTG AAAACTGTAA GGAGACAGAG ATGCTGAACT ACCTCATTGA GTGTTTTGAC
    CGGGTTGGAA TAGAGGAGCG AAAAGCACCA AAGATGTGTA GCCAGCCAAC AGTGAGTCAG
    TTACTGAGCA ACATACGATC GCAGTGCATT TCACATGCTG CTTTGGTGCT ACAGGGATCC
    TTAACGCAAC CAAGGTCATT GCAGCAGCAG TCTTTGCTGG TACCATATAT GCTGTGTAGG
    AATCTCCCAT TTGGCTTCAT CCAAGAACTG GTGAGAACAA CTTACCAAGA TGAAGAGGTG
    TTCAAACAGA TCTTTATCCC CATCCTGCAA GGCCTGGCTG TAGCTTCCAA AGAGTGCTCC
    CTTGATAGTG ACAATTTTAA GTACCCCCTT ATGGCATTAT GTGAACTTTG TGAAATCAAG
    TTTGGAAAAA CACACCCCAT GTGCAGTTTG GTTGTCTCTT TGCCCTTGTG GCTGCCTAAA
    TCTTTAAGCA CGGGTGCAGG AAGAGAGCTA CAGAGACTTT CCTATCTTGG AGCCTTCTTC
    AGTCTCTCTG TCTTTGCTGA AGATGACAAC AAAGTTGTTG AAAAGTACTT CTCAGGACCT
    GCCATCACTC TTGAAAACAC CCGAGTTGTT AGCCAGTCCT TGCAGCATTA CCTGGAACTA
    GCGAGGCAAG AGCTCTTCAA GATCCTGCAT AGTATTTTAC TGAATGGTGA AACTCGAGAG
    GCAGCTCTCA GTTACATGGC AGCTGTTGTC AACGCCAACA TGAAAAAGGC ACAAATGCAG
    ACAGATGATC GTTTGGTGTC TACAGATGGT TTTATGCTCA ACTTCCTATG GGTGCTGCAG
    CAACTAAGTA TGAAGATCAA GCTGGAAACG GTTGATCCCA TGTACATATT TCATCCCAGG
    TGTCGTATTG ACCTCCCGAC AGATGAGACA AGAGTGAAAG CAACAATGGA GGATGTTACA
    GCCTGGATTG CTGAGCTTTA CAGGGATCCG TCTCCATTTT CTGATCCAAA ATTCCCAACA
    GAATGTTTCT TCTTAACCCT GCATGCCCAT CATCTCTCCA TCCTGCCGAG CTGCCGTCGG
    TACATACGTA GACTGCGAGC CATCAGGGAA CTCAACAGGA CTGTTGAAGA TCTGAAAAAC
    AATGAAAGTC AATGGAAGGA TTCTCCTCTG GCTACCAGAC ACAGAGAGAT GCTAAAACGT
    TGCAAGACAC AACTTAAGAA ACTGGTGAGG TGCAAGGCTT GTGCAGATGC TGGTTTGCTG
    GATGAGAACT TTCTCAGGAG ATGTCTGAAT TTCTATGGCA TGGTGATTCA ACTGATGCTT
    CGCATCCTCG ACCCCGCCTA TCCCAACATA AAATTGCCTT TAACACTTGA AGTTCCGAAG
    GTATTTGCAG CATTGCCAGA GTTTTACGTG GAAGATGTTG CTGAATTTTT ATTTTTTATT
    GTACAATATG CTCCTCAGGT GCTTTATGAG CCCTGTACTC AGGACATAGT GATGTTCCTT
    GTTGTGATGC TGTGCAACCA GAACTACATC CGAAATCCTT ATTTAGTTGC TAAGCTGGTG
    GAGGTGATGT TCATGACCAA TCCAGCTGTT CAGCCTCGAA CACAGAAGTT CTTTGAAATG
    ATTGAGAATC ATCCTCTGTC CACTAAATTG TTAGTCCCCT CCTTAATGAA ATTTTACACA
    GATGTTGAGC ATACTGGAGC AACTAGTGAA TTCTATGACA AGTTTACAAT CCGCTACCAC
    ATCAGCACCA TTTTCAAAAG CCTCTGGCAG AACATAGCTC ACCATGGTAC ATTTATGGAA
    GAGTTCAACT CTGGGAAGCA GTTTGTTCGC TACATCAACA TGCTGATAAA TGACACAACT
    TTCTTGCTGG ATGAGAGTCT GGAATCCCTA AAACGTATCC ATGAAGTGCA AGAGGAGATG
    AAGAATAAAG AACAATGGGA CCTGCTGCCT AGGGATCAGC AGCAGGCTCG GCAGTCGCAG
    CTGGCTCAGG ATGAGCGTGT CTCTCGTTCA TATCTTGCCC TGGCAACAGA AACTGTTGAT
    ATGTTCCATA TCCTTACCAA GCAGGTTCAA AAGCCTTTTC TCAGACCTGA ACTTGGACCA
    CGATTGGCTG CTATGTTGAA CTTTAACTTG CAGCAACTAT GTGGCCCGAA GTGCCGTGAC
    CTAAAAGTTG AGAACCCTGA GAAATACGGG TTTGAACCAA AGAAGCTGTT GGACCAGCTG
    ACTGACATTT ATCTACAGCT AGACTGTGCT CGTTTTGCTA AAGCAATTGC TGATGATCAG
    AGGTCCTACA GCAAAGAGCT CTTTGAGGAG GTCATATCAA AGATGAGAAA GGCTGGAATC
    AAGTCAACCA TAGCAATAGA AAAATTCAAA CTGCTGGCAG AGAAAGTGGA AGAGATTGTT
    GCCAAGAATG CCCGTGCAGA AATTGATTAC AGCGATGCTC CGGATGAATT CAGAGATCCA
    CTCATGGACA CTCTGATGAC TGATCCTGTG AGGTTGCCAT CTGGAACTAT TATGGACAGG
    TCAATCATCC TGAGGCATCT GTTGAATTCT TCCACTGATC CCTTCAATCG TCAGACACTG
    ACAGAAAATA TGCTGGAACC AGTGCCAGAA CTTAAAGAGC AAATCCAGGC CTGGATGAGA
    GACAAGCAGA ATGCTGACCA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa18251
    Clone ID Related Accession (Same CDS sequence) XM_004947452.3 , XM_004947452.2
    Accession Version XM_004947452.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4044bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product ubiquitin conjugation factor E4 B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004947452.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGAGC TGAGCGCGGA CGAGATCCGT CGAAGGCGCC TTGCCCGGCT GGCAGGGGGA 
    CCATCGTCCC AGCCCAGCAC ACCGCTCACA TCGCCGCAGA GAGAGACCCC ACCTGGGCCA
    CCTGCAGCAG CTCCGACCCC GGGGCCATCC CATAGCCTCG GCCTAAATGT CCACAGCATG
    ACCCCAGCTA CCTCTCCGAT TGGTGCATCA GGAGTAGCCC ACCGAAGCCA GAGCAGCGAA
    GGGGTGAGTT CGCTCAGCAG TTCTCCATCA AACAGCCTTG AAACACAATC CCAGTCTCTC
    TCTCGGTCTC AGAGTATGGA TATTGATAGT GTCTCCTGTG AAAAAAGTAT GTCCCAGGTA
    GATGTGGATT CAGGAATTGA GAACATGGAA GTGGATGAGA ATGATCGCAG GGAGAAAAGG
    AGTCTGACTG ATAAGGAGGA GCCTCTGTCT GGGTCTGAGG TGTCCGAGGA GCAAGCTTTA
    CAGCTGGTCT GTAAAATCTT CAGAGTCTCT TGGAAGGACC GAGACAGAGA TGTTATCTTC
    CTGAATTCTC TCTCTGCTCA GTTTAAGCAG AACCCAAAAG AAGTGTTTTC GGATTTTAAG
    GACTTGATCG GACAGATCTT GATGGAGGTG CTGATGATGT CCACCCAGTC AAGGGATGAA
    AACCCATTTG CCAGTCTGAC AGCTACGTCA CAGCCAATTG CTGCAACTGC CCGGTCTCCT
    GACAGAAGCC TGATCTTAAA CACGGGCTCC AACCCAGGAA CCAGCCCTAT GTTCTGTAAT
    GTGGGCTCCT TTGGTTCCAG TTCGTTATCC AGTCTCTATG GGACTAGTCC AGCTCCTACT
    ACCAATTTCA CAAGCTATGT ACCAATGACT GGTTCATCTC TTACCCCACC AGCCAGTTCA
    GTTGCCACCA CATCTGTGCC TTCCATTACT GTCAGCTCTC ACCTCACAGC AACCAGCCCT
    TCTTGGACCC CGCCTTCCTC TCCGAGGTAC CGGCCATATA CTGTTGCCCA TTCTGGAGGC
    TTTTCAGCTT CTTCTATCCC ACGTTCCTTG AATATTTCCA TCCCAGCTAG TTCAAGTCCT
    CCAGTCATGG CTGCTAGCCC TCCAGCCATG CTGCTCACTG CACCCAGACA GAGACCCACG
    ACTATGGGTC CGCCTTTGTT TACTTCACCC AGTACCTTGC GCAGGCGTTC ATCTTTACTG
    AACAGGATTC CTTCTAGTAT ATACGACAAC CCTTTCTCCC TCCTCTTCCT TGGCGTTTCT
    GATGTGTCTG AGGACAGTAG TGACGAAGAA AATGAAGATG ATGATTTTTC TTGTGTCCAG
    TTTGGGTCCA GTGTGGGAGG CTCTGGCAGC TCAAGTGTTT GCGATTCCTG CAGTGACCAC
    TTCACCATTG AAAACTGTAA GGAGACAGAG ATGCTGAACT ACCTCATTGA GTGTTTTGAC
    CGGGTTGGAA TAGAGGAGCG AAAAGCACCA AAGATGTGTA GCCAGCCAAC AGTGAGTCAG
    TTACTGAGCA ACATACGATC GCAGTGCATT TCACATGCTG CTTTGGTGCT ACAGGGATCC
    TTAACGCAAC CAAGGTCATT GCAGCAGCAG TCTTTGCTGG TACCATATAT GCTGTGTAGG
    AATCTCCCAT TTGGCTTCAT CCAAGAACTG GTGAGAACAA CTTACCAAGA TGAAGAGGTG
    TTCAAACAGA TCTTTATCCC CATCCTGCAA GGCCTGGCTG TAGCTTCCAA AGAGTGCTCC
    CTTGATAGTG ACAATTTTAA GTACCCCCTT ATGGCATTAT GTGAACTTTG TGAAATCAAG
    TTTGGAAAAA CACACCCCAT GTGCAGTTTG GTTGTCTCTT TGCCCTTGTG GCTGCCTAAA
    TCTTTAAGCA CGGGTGCAGG AAGAGAGCTA CAGAGACTTT CCTATCTTGG AGCCTTCTTC
    AGTCTCTCTG TCTTTGCTGA AGATGACAAC AAAGTTGTTG AAAAGTACTT CTCAGGACCT
    GCCATCACTC TTGAAAACAC CCGAGTTGTT AGCCAGTCCT TGCAGCATTA CCTGGAACTA
    GCGAGGCAAG AGCTCTTCAA GATCCTGCAT AGTATTTTAC TGAATGGTGA AACTCGAGAG
    GCAGCTCTCA GTTACATGGC AGCTGTTGTC AACGCCAACA TGAAAAAGGC ACAAATGCAG
    ACAGATGATC GTTTGGTGTC TACAGATGGT TTTATGCTCA ACTTCCTATG GGTGCTGCAG
    CAACTAAGTA TGAAGATCAA GCTGGAAACG GTTGATCCCA TGTACATATT TCATCCCAGG
    TGTCGTATTG ACCTCCCGAC AGATGAGACA AGAGTGAAAG CAACAATGGA GGATGTTACA
    GCCTGGATTG CTGAGCTTTA CAGGGATCCG TCTCCATTTT CTGATCCAAA ATTCCCAACA
    GAATGTTTCT TCTTAACCCT GCATGCCCAT CATCTCTCCA TCCTGCCGAG CTGCCGTCGG
    TACATACGTA GACTGCGAGC CATCAGGGAA CTCAACAGGA CTGTTGAAGA TCTGAAAAAC
    AATGAAAGTC AATGGAAGGA TTCTCCTCTG GCTACCAGAC ACAGAGAGAT GCTAAAACGT
    TGCAAGACAC AACTTAAGAA ACTGGTGAGG TGCAAGGCTT GTGCAGATGC TGGTTTGCTG
    GATGAGAACT TTCTCAGGAG ATGTCTGAAT TTCTATGGCA TGGTGATTCA ACTGATGCTT
    CGCATCCTCG ACCCCGCCTA TCCCAACATA AAATTGCCTT TAACACTTGA AGTTCCGAAG
    GTATTTGCAG CATTGCCAGA GTTTTACGTG GAAGATGTTG CTGAATTTTT ATTTTTTATT
    GTACAATATG CTCCTCAGGT GCTTTATGAG CCCTGTACTC AGGACATAGT GATGTTCCTT
    GTTGTGATGC TGTGCAACCA GAACTACATC CGAAATCCTT ATTTAGTTGC TAAGCTGGTG
    GAGGTGATGT TCATGACCAA TCCAGCTGTT CAGCCTCGAA CACAGAAGTT CTTTGAAATG
    ATTGAGAATC ATCCTCTGTC CACTAAATTG TTAGTCCCCT CCTTAATGAA ATTTTACACA
    GATGTTGAGC ATACTGGAGC AACTAGTGAA TTCTATGACA AGTTTACAAT CCGCTACCAC
    ATCAGCACCA TTTTCAAAAG CCTCTGGCAG AACATAGCTC ACCATGGTAC ATTTATGGAA
    GAGTTCAACT CTGGGAAGCA GTTTGTTCGC TACATCAACA TGCTGATAAA TGACACAACT
    TTCTTGCTGG ATGAGAGTCT GGAATCCCTA AAACGTATCC ATGAAGTGCA AGAGGAGATG
    AAGAATAAAG AACAATGGGA CCTGCTGCCT AGGGATCAGC AGCAGGCTCG GCAGTCGCAG
    CTGGCTCAGG ATGAGCGTGT CTCTCGTTCA TATCTTGCCC TGGCAACAGA AACTGTTGAT
    ATGTTCCATA TCCTTACCAA GCAGGTTCAA AAGCCTTTTC TCAGACCTGA ACTTGGACCA
    CGATTGGCTG CTATGTTGAA CTTTAACTTG CAGCAACTAT GTGGCCCGAA GTGCCGTGAC
    CTAAAAGTTG AGAACCCTGA GAAATACGGG TTTGAACCAA AGAAGCTGTT GGACCAGCTG
    ACTGACATTT ATCTACAGCT AGACTGTGCT CGTTTTGCTA AAGCAATTGC TGATGATCAG
    AGGTCCTACA GCAAAGAGCT CTTTGAGGAG GTCATATCAA AGATGAGAAA GGCTGGAATC
    AAGTCAACCA TAGCAATAGA AAAATTCAAA CTGCTGGCAG AGAAAGTGGA AGAGATTGTT
    GCCAAGAATG CCCGTGCAGA AATTGATTAC AGCGATGCTC CGGATGAATT CAGAGATCCA
    CTCATGGACA CTCTGATGAC TGATCCTGTG AGGTTGCCAT CTGGAACTAT TATGGACAGG
    TCAATCATCC TGAGGCATCT GTTGAATTCT TCCACTGATC CCTTCAATCG TCAGACACTG
    ACAGAAAATA TGCTGGAACC AGTGCCAGAA CTTAAAGAGC AAATCCAGGC CTGGATGAGA
    GACAAGCAGA ATGCTGACCA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004947509.1
    CDS139..4182
    Translation

    Target ORF information:

    RefSeq Version XM_004947452.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ubiquitination factor E4B (UBE4B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004947452.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGAGC TGAGCGCGGA CGAGATCCGT CGAAGGCGCC TTGCCCGGCT GGCAGGGGGA 
    CCATCGTCCC AGCCCAGCAC ACCGCTCACA TCGCCGCAGA GAGAGACCCC ACCTGGGCCA
    CCTGCAGCAG CTCCGACCCC GGGGCCATCC CATAGCCTCG GCCTAAATGT CCACAGCATG
    ACCCCAGCTA CCTCTCCGAT TGGTGCATCA GGAGTAGCCC ACCGAAGCCA GAGCAGCGAA
    GGGGTGAGTT CGCTCAGCAG TTCTCCATCA AACAGCCTTG AAACACAATC CCAGTCTCTC
    TCTCGGTCTC AGAGTATGGA TATTGATAGT GTCTCCTGTG AAAAAAGTAT GTCCCAGGTA
    GATGTGGATT CAGGAATTGA GAACATGGAA GTGGATGAGA ATGATCGCAG GGAGAAAAGG
    AGTCTGACTG ATAAGGAGGA GCCTCTGTCT GGGTCTGAGG TGTCCGAGGA GCAAGCTTTA
    CAGCTGGTCT GTAAAATCTT CAGAGTCTCT TGGAAGGACC GAGACAGAGA TGTTATCTTC
    CTGAATTCTC TCTCTGCTCA GTTTAAGCAG AACCCAAAAG AAGTGTTTTC GGATTTTAAG
    GACTTGATCG GACAGATCTT GATGGAGGTG CTGATGATGT CCACCCAGTC AAGGGATGAA
    AACCCATTTG CCAGTCTGAC AGCTACGTCA CAGCCAATTG CTGCAACTGC CCGGTCTCCT
    GACAGAAGCC TGATCTTAAA CACGGGCTCC AACCCAGGAA CCAGCCCTAT GTTCTGTAAT
    GTGGGCTCCT TTGGTTCCAG TTCGTTATCC AGTCTCTATG GGACTAGTCC AGCTCCTACT
    ACCAATTTCA CAAGCTATGT ACCAATGACT GGTTCATCTC TTACCCCACC AGCCAGTTCA
    GTTGCCACCA CATCTGTGCC TTCCATTACT GTCAGCTCTC ACCTCACAGC AACCAGCCCT
    TCTTGGACCC CGCCTTCCTC TCCGAGGTAC CGGCCATATA CTGTTGCCCA TTCTGGAGGC
    TTTTCAGCTT CTTCTATCCC ACGTTCCTTG AATATTTCCA TCCCAGCTAG TTCAAGTCCT
    CCAGTCATGG CTGCTAGCCC TCCAGCCATG CTGCTCACTG CACCCAGACA GAGACCCACG
    ACTATGGGTC CGCCTTTGTT TACTTCACCC AGTACCTTGC GCAGGCGTTC ATCTTTACTG
    AACAGGATTC CTTCTAGTAT ATACGACAAC CCTTTCTCCC TCCTCTTCCT TGGCGTTTCT
    GATGTGTCTG AGGACAGTAG TGACGAAGAA AATGAAGATG ATGATTTTTC TTGTGTCCAG
    TTTGGGTCCA GTGTGGGAGG CTCTGGCAGC TCAAGTGTTT GCGATTCCTG CAGTGACCAC
    TTCACCATTG AAAACTGTAA GGAGACAGAG ATGCTGAACT ACCTCATTGA GTGTTTTGAC
    CGGGTTGGAA TAGAGGAGCG AAAAGCACCA AAGATGTGTA GCCAGCCAAC AGTGAGTCAG
    TTACTGAGCA ACATACGATC GCAGTGCATT TCACATGCTG CTTTGGTGCT ACAGGGATCC
    TTAACGCAAC CAAGGTCATT GCAGCAGCAG TCTTTGCTGG TACCATATAT GCTGTGTAGG
    AATCTCCCAT TTGGCTTCAT CCAAGAACTG GTGAGAACAA CTTACCAAGA TGAAGAGGTG
    TTCAAACAGA TCTTTATCCC CATCCTGCAA GGCCTGGCTG TAGCTTCCAA AGAGTGCTCC
    CTTGATAGTG ACAATTTTAA GTACCCCCTT ATGGCATTAT GTGAACTTTG TGAAATCAAG
    TTTGGAAAAA CACACCCCAT GTGCAGTTTG GTTGTCTCTT TGCCCTTGTG GCTGCCTAAA
    TCTTTAAGCA CGGGTGCAGG AAGAGAGCTA CAGAGACTTT CCTATCTTGG AGCCTTCTTC
    AGTCTCTCTG TCTTTGCTGA AGATGACAAC AAAGTTGTTG AAAAGTACTT CTCAGGACCT
    GCCATCACTC TTGAAAACAC CCGAGTTGTT AGCCAGTCCT TGCAGCATTA CCTGGAACTA
    GCGAGGCAAG AGCTCTTCAA GATCCTGCAT AGTATTTTAC TGAATGGTGA AACTCGAGAG
    GCAGCTCTCA GTTACATGGC AGCTGTTGTC AACGCCAACA TGAAAAAGGC ACAAATGCAG
    ACAGATGATC GTTTGGTGTC TACAGATGGT TTTATGCTCA ACTTCCTATG GGTGCTGCAG
    CAACTAAGTA TGAAGATCAA GCTGGAAACG GTTGATCCCA TGTACATATT TCATCCCAGG
    TGTCGTATTG ACCTCCCGAC AGATGAGACA AGAGTGAAAG CAACAATGGA GGATGTTACA
    GCCTGGATTG CTGAGCTTTA CAGGGATCCG TCTCCATTTT CTGATCCAAA ATTCCCAACA
    GAATGTTTCT TCTTAACCCT GCATGCCCAT CATCTCTCCA TCCTGCCGAG CTGCCGTCGG
    TACATACGTA GACTGCGAGC CATCAGGGAA CTCAACAGGA CTGTTGAAGA TCTGAAAAAC
    AATGAAAGTC AATGGAAGGA TTCTCCTCTG GCTACCAGAC ACAGAGAGAT GCTAAAACGT
    TGCAAGACAC AACTTAAGAA ACTGGTGAGG TGCAAGGCTT GTGCAGATGC TGGTTTGCTG
    GATGAGAACT TTCTCAGGAG ATGTCTGAAT TTCTATGGCA TGGTGATTCA ACTGATGCTT
    CGCATCCTCG ACCCCGCCTA TCCCAACATA AAATTGCCTT TAACACTTGA AGTTCCGAAG
    GTATTTGCAG CATTGCCAGA GTTTTACGTG GAAGATGTTG CTGAATTTTT ATTTTTTATT
    GTACAATATG CTCCTCAGGT GCTTTATGAG CCCTGTACTC AGGACATAGT GATGTTCCTT
    GTTGTGATGC TGTGCAACCA GAACTACATC CGAAATCCTT ATTTAGTTGC TAAGCTGGTG
    GAGGTGATGT TCATGACCAA TCCAGCTGTT CAGCCTCGAA CACAGAAGTT CTTTGAAATG
    ATTGAGAATC ATCCTCTGTC CACTAAATTG TTAGTCCCCT CCTTAATGAA ATTTTACACA
    GATGTTGAGC ATACTGGAGC AACTAGTGAA TTCTATGACA AGTTTACAAT CCGCTACCAC
    ATCAGCACCA TTTTCAAAAG CCTCTGGCAG AACATAGCTC ACCATGGTAC ATTTATGGAA
    GAGTTCAACT CTGGGAAGCA GTTTGTTCGC TACATCAACA TGCTGATAAA TGACACAACT
    TTCTTGCTGG ATGAGAGTCT GGAATCCCTA AAACGTATCC ATGAAGTGCA AGAGGAGATG
    AAGAATAAAG AACAATGGGA CCTGCTGCCT AGGGATCAGC AGCAGGCTCG GCAGTCGCAG
    CTGGCTCAGG ATGAGCGTGT CTCTCGTTCA TATCTTGCCC TGGCAACAGA AACTGTTGAT
    ATGTTCCATA TCCTTACCAA GCAGGTTCAA AAGCCTTTTC TCAGACCTGA ACTTGGACCA
    CGATTGGCTG CTATGTTGAA CTTTAACTTG CAGCAACTAT GTGGCCCGAA GTGCCGTGAC
    CTAAAAGTTG AGAACCCTGA GAAATACGGG TTTGAACCAA AGAAGCTGTT GGACCAGCTG
    ACTGACATTT ATCTACAGCT AGACTGTGCT CGTTTTGCTA AAGCAATTGC TGATGATCAG
    AGGTCCTACA GCAAAGAGCT CTTTGAGGAG GTCATATCAA AGATGAGAAA GGCTGGAATC
    AAGTCAACCA TAGCAATAGA AAAATTCAAA CTGCTGGCAG AGAAAGTGGA AGAGATTGTT
    GCCAAGAATG CCCGTGCAGA AATTGATTAC AGCGATGCTC CGGATGAATT CAGAGATCCA
    CTCATGGACA CTCTGATGAC TGATCCTGTG AGGTTGCCAT CTGGAACTAT TATGGACAGG
    TCAATCATCC TGAGGCATCT GTTGAATTCT TCCACTGATC CCTTCAATCG TCAGACACTG
    ACAGAAAATA TGCTGGAACC AGTGCCAGAA CTTAAAGAGC AAATCCAGGC CTGGATGAGA
    GACAAGCAGA ATGCTGACCA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa18252
    Clone ID Related Accession (Same CDS sequence) XM_004947453.3 , XM_004947453.2
    Accession Version XM_004947453.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4041bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ubiquitin conjugation factor E4 B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006108.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004947453.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGAGC TGAGCGCGGA CGAGATCCGT CGAAGGCGCC TTGCCCGGCT GGCAGGGGGA 
    CCATCGTCCC AGCCCAGCAC ACCGCTCACA TCGCCGCAGA GAGAGACCCC ACCTGGGCCA
    CCTGCAGCAG CTCCGACCCC GGGGCCATCC CATAGCCTCG GCCTAAATGT CCACAGCATG
    ACCCCAGCTA CCTCTCCGAT TGGTGCATCA GGAGTAGCCC ACCGAAGCCA GAGCAGCGAA
    GGGGTGAGTT CGCTCAGCAG TTCTCCATCA AACAGCCTTG AAACACAATC CCAGTCTCTC
    TCTCGGTCTC AGAGTATGGA TATTGATAGT GTCTCCTGTG AAAAAAGTAT GTCCCAGGTA
    GATGTGGATT CAGGAATTGA GAACATGGAA GTGGATGAGA ATGATCGCAG GGAGAAAAGG
    AGTCTGACTG ATAAGGAGCC TCTGTCTGGG TCTGAGGTGT CCGAGGAGCA AGCTTTACAG
    CTGGTCTGTA AAATCTTCAG AGTCTCTTGG AAGGACCGAG ACAGAGATGT TATCTTCCTG
    AATTCTCTCT CTGCTCAGTT TAAGCAGAAC CCAAAAGAAG TGTTTTCGGA TTTTAAGGAC
    TTGATCGGAC AGATCTTGAT GGAGGTGCTG ATGATGTCCA CCCAGTCAAG GGATGAAAAC
    CCATTTGCCA GTCTGACAGC TACGTCACAG CCAATTGCTG CAACTGCCCG GTCTCCTGAC
    AGAAGCCTGA TCTTAAACAC GGGCTCCAAC CCAGGAACCA GCCCTATGTT CTGTAATGTG
    GGCTCCTTTG GTTCCAGTTC GTTATCCAGT CTCTATGGGA CTAGTCCAGC TCCTACTACC
    AATTTCACAA GCTATGTACC AATGACTGGT TCATCTCTTA CCCCACCAGC CAGTTCAGTT
    GCCACCACAT CTGTGCCTTC CATTACTGTC AGCTCTCACC TCACAGCAAC CAGCCCTTCT
    TGGACCCCGC CTTCCTCTCC GAGGTACCGG CCATATACTG TTGCCCATTC TGGAGGCTTT
    TCAGCTTCTT CTATCCCACG TTCCTTGAAT ATTTCCATCC CAGCTAGTTC AAGTCCTCCA
    GTCATGGCTG CTAGCCCTCC AGCCATGCTG CTCACTGCAC CCAGACAGAG ACCCACGACT
    ATGGGTCCGC CTTTGTTTAC TTCACCCAGT ACCTTGCGCA GGCGTTCATC TTTACTGAAC
    AGGATTCCTT CTAGTATATA CGACAACCCT TTCTCCCTCC TCTTCCTTGG CGTTTCTGAT
    GTGTCTGAGG ACAGTAGTGA CGAAGAAAAT GAAGATGATG ATTTTTCTTG TGTCCAGTTT
    GGGTCCAGTG TGGGAGGCTC TGGCAGCTCA AGTGTTTGCG ATTCCTGCAG TGACCACTTC
    ACCATTGAAA ACTGTAAGGA GACAGAGATG CTGAACTACC TCATTGAGTG TTTTGACCGG
    GTTGGAATAG AGGAGCGAAA AGCACCAAAG ATGTGTAGCC AGCCAACAGT GAGTCAGTTA
    CTGAGCAACA TACGATCGCA GTGCATTTCA CATGCTGCTT TGGTGCTACA GGGATCCTTA
    ACGCAACCAA GGTCATTGCA GCAGCAGTCT TTGCTGGTAC CATATATGCT GTGTAGGAAT
    CTCCCATTTG GCTTCATCCA AGAACTGGTG AGAACAACTT ACCAAGATGA AGAGGTGTTC
    AAACAGATCT TTATCCCCAT CCTGCAAGGC CTGGCTGTAG CTTCCAAAGA GTGCTCCCTT
    GATAGTGACA ATTTTAAGTA CCCCCTTATG GCATTATGTG AACTTTGTGA AATCAAGTTT
    GGAAAAACAC ACCCCATGTG CAGTTTGGTT GTCTCTTTGC CCTTGTGGCT GCCTAAATCT
    TTAAGCACGG GTGCAGGAAG AGAGCTACAG AGACTTTCCT ATCTTGGAGC CTTCTTCAGT
    CTCTCTGTCT TTGCTGAAGA TGACAACAAA GTTGTTGAAA AGTACTTCTC AGGACCTGCC
    ATCACTCTTG AAAACACCCG AGTTGTTAGC CAGTCCTTGC AGCATTACCT GGAACTAGCG
    AGGCAAGAGC TCTTCAAGAT CCTGCATAGT ATTTTACTGA ATGGTGAAAC TCGAGAGGCA
    GCTCTCAGTT ACATGGCAGC TGTTGTCAAC GCCAACATGA AAAAGGCACA AATGCAGACA
    GATGATCGTT TGGTGTCTAC AGATGGTTTT ATGCTCAACT TCCTATGGGT GCTGCAGCAA
    CTAAGTATGA AGATCAAGCT GGAAACGGTT GATCCCATGT ACATATTTCA TCCCAGGTGT
    CGTATTGACC TCCCGACAGA TGAGACAAGA GTGAAAGCAA CAATGGAGGA TGTTACAGCC
    TGGATTGCTG AGCTTTACAG GGATCCGTCT CCATTTTCTG ATCCAAAATT CCCAACAGAA
    TGTTTCTTCT TAACCCTGCA TGCCCATCAT CTCTCCATCC TGCCGAGCTG CCGTCGGTAC
    ATACGTAGAC TGCGAGCCAT CAGGGAACTC AACAGGACTG TTGAAGATCT GAAAAACAAT
    GAAAGTCAAT GGAAGGATTC TCCTCTGGCT ACCAGACACA GAGAGATGCT AAAACGTTGC
    AAGACACAAC TTAAGAAACT GGTGAGGTGC AAGGCTTGTG CAGATGCTGG TTTGCTGGAT
    GAGAACTTTC TCAGGAGATG TCTGAATTTC TATGGCATGG TGATTCAACT GATGCTTCGC
    ATCCTCGACC CCGCCTATCC CAACATAAAA TTGCCTTTAA CACTTGAAGT TCCGAAGGTA
    TTTGCAGCAT TGCCAGAGTT TTACGTGGAA GATGTTGCTG AATTTTTATT TTTTATTGTA
    CAATATGCTC CTCAGGTGCT TTATGAGCCC TGTACTCAGG ACATAGTGAT GTTCCTTGTT
    GTGATGCTGT GCAACCAGAA CTACATCCGA AATCCTTATT TAGTTGCTAA GCTGGTGGAG
    GTGATGTTCA TGACCAATCC AGCTGTTCAG CCTCGAACAC AGAAGTTCTT TGAAATGATT
    GAGAATCATC CTCTGTCCAC TAAATTGTTA GTCCCCTCCT TAATGAAATT TTACACAGAT
    GTTGAGCATA CTGGAGCAAC TAGTGAATTC TATGACAAGT TTACAATCCG CTACCACATC
    AGCACCATTT TCAAAAGCCT CTGGCAGAAC ATAGCTCACC ATGGTACATT TATGGAAGAG
    TTCAACTCTG GGAAGCAGTT TGTTCGCTAC ATCAACATGC TGATAAATGA CACAACTTTC
    TTGCTGGATG AGAGTCTGGA ATCCCTAAAA CGTATCCATG AAGTGCAAGA GGAGATGAAG
    AATAAAGAAC AATGGGACCT GCTGCCTAGG GATCAGCAGC AGGCTCGGCA GTCGCAGCTG
    GCTCAGGATG AGCGTGTCTC TCGTTCATAT CTTGCCCTGG CAACAGAAAC TGTTGATATG
    TTCCATATCC TTACCAAGCA GGTTCAAAAG CCTTTTCTCA GACCTGAACT TGGACCACGA
    TTGGCTGCTA TGTTGAACTT TAACTTGCAG CAACTATGTG GCCCGAAGTG CCGTGACCTA
    AAAGTTGAGA ACCCTGAGAA ATACGGGTTT GAACCAAAGA AGCTGTTGGA CCAGCTGACT
    GACATTTATC TACAGCTAGA CTGTGCTCGT TTTGCTAAAG CAATTGCTGA TGATCAGAGG
    TCCTACAGCA AAGAGCTCTT TGAGGAGGTC ATATCAAAGA TGAGAAAGGC TGGAATCAAG
    TCAACCATAG CAATAGAAAA ATTCAAACTG CTGGCAGAGA AAGTGGAAGA GATTGTTGCC
    AAGAATGCCC GTGCAGAAAT TGATTACAGC GATGCTCCGG ATGAATTCAG AGATCCACTC
    ATGGACACTC TGATGACTGA TCCTGTGAGG TTGCCATCTG GAACTATTAT GGACAGGTCA
    ATCATCCTGA GGCATCTGTT GAATTCTTCC ACTGATCCCT TCAATCGTCA GACACTGACA
    GAAAATATGC TGGAACCAGT GCCAGAACTT AAAGAGCAAA TCCAGGCCTG GATGAGAGAC
    AAGCAGAATG CTGACCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004947510.1
    CDS117..4157
    Translation

    Target ORF information:

    RefSeq Version XM_004947453.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ubiquitination factor E4B (UBE4B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004947453.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGAGC TGAGCGCGGA CGAGATCCGT CGAAGGCGCC TTGCCCGGCT GGCAGGGGGA 
    CCATCGTCCC AGCCCAGCAC ACCGCTCACA TCGCCGCAGA GAGAGACCCC ACCTGGGCCA
    CCTGCAGCAG CTCCGACCCC GGGGCCATCC CATAGCCTCG GCCTAAATGT CCACAGCATG
    ACCCCAGCTA CCTCTCCGAT TGGTGCATCA GGAGTAGCCC ACCGAAGCCA GAGCAGCGAA
    GGGGTGAGTT CGCTCAGCAG TTCTCCATCA AACAGCCTTG AAACACAATC CCAGTCTCTC
    TCTCGGTCTC AGAGTATGGA TATTGATAGT GTCTCCTGTG AAAAAAGTAT GTCCCAGGTA
    GATGTGGATT CAGGAATTGA GAACATGGAA GTGGATGAGA ATGATCGCAG GGAGAAAAGG
    AGTCTGACTG ATAAGGAGCC TCTGTCTGGG TCTGAGGTGT CCGAGGAGCA AGCTTTACAG
    CTGGTCTGTA AAATCTTCAG AGTCTCTTGG AAGGACCGAG ACAGAGATGT TATCTTCCTG
    AATTCTCTCT CTGCTCAGTT TAAGCAGAAC CCAAAAGAAG TGTTTTCGGA TTTTAAGGAC
    TTGATCGGAC AGATCTTGAT GGAGGTGCTG ATGATGTCCA CCCAGTCAAG GGATGAAAAC
    CCATTTGCCA GTCTGACAGC TACGTCACAG CCAATTGCTG CAACTGCCCG GTCTCCTGAC
    AGAAGCCTGA TCTTAAACAC GGGCTCCAAC CCAGGAACCA GCCCTATGTT CTGTAATGTG
    GGCTCCTTTG GTTCCAGTTC GTTATCCAGT CTCTATGGGA CTAGTCCAGC TCCTACTACC
    AATTTCACAA GCTATGTACC AATGACTGGT TCATCTCTTA CCCCACCAGC CAGTTCAGTT
    GCCACCACAT CTGTGCCTTC CATTACTGTC AGCTCTCACC TCACAGCAAC CAGCCCTTCT
    TGGACCCCGC CTTCCTCTCC GAGGTACCGG CCATATACTG TTGCCCATTC TGGAGGCTTT
    TCAGCTTCTT CTATCCCACG TTCCTTGAAT ATTTCCATCC CAGCTAGTTC AAGTCCTCCA
    GTCATGGCTG CTAGCCCTCC AGCCATGCTG CTCACTGCAC CCAGACAGAG ACCCACGACT
    ATGGGTCCGC CTTTGTTTAC TTCACCCAGT ACCTTGCGCA GGCGTTCATC TTTACTGAAC
    AGGATTCCTT CTAGTATATA CGACAACCCT TTCTCCCTCC TCTTCCTTGG CGTTTCTGAT
    GTGTCTGAGG ACAGTAGTGA CGAAGAAAAT GAAGATGATG ATTTTTCTTG TGTCCAGTTT
    GGGTCCAGTG TGGGAGGCTC TGGCAGCTCA AGTGTTTGCG ATTCCTGCAG TGACCACTTC
    ACCATTGAAA ACTGTAAGGA GACAGAGATG CTGAACTACC TCATTGAGTG TTTTGACCGG
    GTTGGAATAG AGGAGCGAAA AGCACCAAAG ATGTGTAGCC AGCCAACAGT GAGTCAGTTA
    CTGAGCAACA TACGATCGCA GTGCATTTCA CATGCTGCTT TGGTGCTACA GGGATCCTTA
    ACGCAACCAA GGTCATTGCA GCAGCAGTCT TTGCTGGTAC CATATATGCT GTGTAGGAAT
    CTCCCATTTG GCTTCATCCA AGAACTGGTG AGAACAACTT ACCAAGATGA AGAGGTGTTC
    AAACAGATCT TTATCCCCAT CCTGCAAGGC CTGGCTGTAG CTTCCAAAGA GTGCTCCCTT
    GATAGTGACA ATTTTAAGTA CCCCCTTATG GCATTATGTG AACTTTGTGA AATCAAGTTT
    GGAAAAACAC ACCCCATGTG CAGTTTGGTT GTCTCTTTGC CCTTGTGGCT GCCTAAATCT
    TTAAGCACGG GTGCAGGAAG AGAGCTACAG AGACTTTCCT ATCTTGGAGC CTTCTTCAGT
    CTCTCTGTCT TTGCTGAAGA TGACAACAAA GTTGTTGAAA AGTACTTCTC AGGACCTGCC
    ATCACTCTTG AAAACACCCG AGTTGTTAGC CAGTCCTTGC AGCATTACCT GGAACTAGCG
    AGGCAAGAGC TCTTCAAGAT CCTGCATAGT ATTTTACTGA ATGGTGAAAC TCGAGAGGCA
    GCTCTCAGTT ACATGGCAGC TGTTGTCAAC GCCAACATGA AAAAGGCACA AATGCAGACA
    GATGATCGTT TGGTGTCTAC AGATGGTTTT ATGCTCAACT TCCTATGGGT GCTGCAGCAA
    CTAAGTATGA AGATCAAGCT GGAAACGGTT GATCCCATGT ACATATTTCA TCCCAGGTGT
    CGTATTGACC TCCCGACAGA TGAGACAAGA GTGAAAGCAA CAATGGAGGA TGTTACAGCC
    TGGATTGCTG AGCTTTACAG GGATCCGTCT CCATTTTCTG ATCCAAAATT CCCAACAGAA
    TGTTTCTTCT TAACCCTGCA TGCCCATCAT CTCTCCATCC TGCCGAGCTG CCGTCGGTAC
    ATACGTAGAC TGCGAGCCAT CAGGGAACTC AACAGGACTG TTGAAGATCT GAAAAACAAT
    GAAAGTCAAT GGAAGGATTC TCCTCTGGCT ACCAGACACA GAGAGATGCT AAAACGTTGC
    AAGACACAAC TTAAGAAACT GGTGAGGTGC AAGGCTTGTG CAGATGCTGG TTTGCTGGAT
    GAGAACTTTC TCAGGAGATG TCTGAATTTC TATGGCATGG TGATTCAACT GATGCTTCGC
    ATCCTCGACC CCGCCTATCC CAACATAAAA TTGCCTTTAA CACTTGAAGT TCCGAAGGTA
    TTTGCAGCAT TGCCAGAGTT TTACGTGGAA GATGTTGCTG AATTTTTATT TTTTATTGTA
    CAATATGCTC CTCAGGTGCT TTATGAGCCC TGTACTCAGG ACATAGTGAT GTTCCTTGTT
    GTGATGCTGT GCAACCAGAA CTACATCCGA AATCCTTATT TAGTTGCTAA GCTGGTGGAG
    GTGATGTTCA TGACCAATCC AGCTGTTCAG CCTCGAACAC AGAAGTTCTT TGAAATGATT
    GAGAATCATC CTCTGTCCAC TAAATTGTTA GTCCCCTCCT TAATGAAATT TTACACAGAT
    GTTGAGCATA CTGGAGCAAC TAGTGAATTC TATGACAAGT TTACAATCCG CTACCACATC
    AGCACCATTT TCAAAAGCCT CTGGCAGAAC ATAGCTCACC ATGGTACATT TATGGAAGAG
    TTCAACTCTG GGAAGCAGTT TGTTCGCTAC ATCAACATGC TGATAAATGA CACAACTTTC
    TTGCTGGATG AGAGTCTGGA ATCCCTAAAA CGTATCCATG AAGTGCAAGA GGAGATGAAG
    AATAAAGAAC AATGGGACCT GCTGCCTAGG GATCAGCAGC AGGCTCGGCA GTCGCAGCTG
    GCTCAGGATG AGCGTGTCTC TCGTTCATAT CTTGCCCTGG CAACAGAAAC TGTTGATATG
    TTCCATATCC TTACCAAGCA GGTTCAAAAG CCTTTTCTCA GACCTGAACT TGGACCACGA
    TTGGCTGCTA TGTTGAACTT TAACTTGCAG CAACTATGTG GCCCGAAGTG CCGTGACCTA
    AAAGTTGAGA ACCCTGAGAA ATACGGGTTT GAACCAAAGA AGCTGTTGGA CCAGCTGACT
    GACATTTATC TACAGCTAGA CTGTGCTCGT TTTGCTAAAG CAATTGCTGA TGATCAGAGG
    TCCTACAGCA AAGAGCTCTT TGAGGAGGTC ATATCAAAGA TGAGAAAGGC TGGAATCAAG
    TCAACCATAG CAATAGAAAA ATTCAAACTG CTGGCAGAGA AAGTGGAAGA GATTGTTGCC
    AAGAATGCCC GTGCAGAAAT TGATTACAGC GATGCTCCGG ATGAATTCAG AGATCCACTC
    ATGGACACTC TGATGACTGA TCCTGTGAGG TTGCCATCTG GAACTATTAT GGACAGGTCA
    ATCATCCTGA GGCATCTGTT GAATTCTTCC ACTGATCCCT TCAATCGTCA GACACTGACA
    GAAAATATGC TGGAACCAGT GCCAGAACTT AAAGAGCAAA TCCAGGCCTG GATGAGAGAC
    AAGCAGAATG CTGACCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa18252
    Clone ID Related Accession (Same CDS sequence) XM_004947453.3 , XM_004947453.2
    Accession Version XM_004947453.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4041bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product ubiquitin conjugation factor E4 B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004947453.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGAGC TGAGCGCGGA CGAGATCCGT CGAAGGCGCC TTGCCCGGCT GGCAGGGGGA 
    CCATCGTCCC AGCCCAGCAC ACCGCTCACA TCGCCGCAGA GAGAGACCCC ACCTGGGCCA
    CCTGCAGCAG CTCCGACCCC GGGGCCATCC CATAGCCTCG GCCTAAATGT CCACAGCATG
    ACCCCAGCTA CCTCTCCGAT TGGTGCATCA GGAGTAGCCC ACCGAAGCCA GAGCAGCGAA
    GGGGTGAGTT CGCTCAGCAG TTCTCCATCA AACAGCCTTG AAACACAATC CCAGTCTCTC
    TCTCGGTCTC AGAGTATGGA TATTGATAGT GTCTCCTGTG AAAAAAGTAT GTCCCAGGTA
    GATGTGGATT CAGGAATTGA GAACATGGAA GTGGATGAGA ATGATCGCAG GGAGAAAAGG
    AGTCTGACTG ATAAGGAGCC TCTGTCTGGG TCTGAGGTGT CCGAGGAGCA AGCTTTACAG
    CTGGTCTGTA AAATCTTCAG AGTCTCTTGG AAGGACCGAG ACAGAGATGT TATCTTCCTG
    AATTCTCTCT CTGCTCAGTT TAAGCAGAAC CCAAAAGAAG TGTTTTCGGA TTTTAAGGAC
    TTGATCGGAC AGATCTTGAT GGAGGTGCTG ATGATGTCCA CCCAGTCAAG GGATGAAAAC
    CCATTTGCCA GTCTGACAGC TACGTCACAG CCAATTGCTG CAACTGCCCG GTCTCCTGAC
    AGAAGCCTGA TCTTAAACAC GGGCTCCAAC CCAGGAACCA GCCCTATGTT CTGTAATGTG
    GGCTCCTTTG GTTCCAGTTC GTTATCCAGT CTCTATGGGA CTAGTCCAGC TCCTACTACC
    AATTTCACAA GCTATGTACC AATGACTGGT TCATCTCTTA CCCCACCAGC CAGTTCAGTT
    GCCACCACAT CTGTGCCTTC CATTACTGTC AGCTCTCACC TCACAGCAAC CAGCCCTTCT
    TGGACCCCGC CTTCCTCTCC GAGGTACCGG CCATATACTG TTGCCCATTC TGGAGGCTTT
    TCAGCTTCTT CTATCCCACG TTCCTTGAAT ATTTCCATCC CAGCTAGTTC AAGTCCTCCA
    GTCATGGCTG CTAGCCCTCC AGCCATGCTG CTCACTGCAC CCAGACAGAG ACCCACGACT
    ATGGGTCCGC CTTTGTTTAC TTCACCCAGT ACCTTGCGCA GGCGTTCATC TTTACTGAAC
    AGGATTCCTT CTAGTATATA CGACAACCCT TTCTCCCTCC TCTTCCTTGG CGTTTCTGAT
    GTGTCTGAGG ACAGTAGTGA CGAAGAAAAT GAAGATGATG ATTTTTCTTG TGTCCAGTTT
    GGGTCCAGTG TGGGAGGCTC TGGCAGCTCA AGTGTTTGCG ATTCCTGCAG TGACCACTTC
    ACCATTGAAA ACTGTAAGGA GACAGAGATG CTGAACTACC TCATTGAGTG TTTTGACCGG
    GTTGGAATAG AGGAGCGAAA AGCACCAAAG ATGTGTAGCC AGCCAACAGT GAGTCAGTTA
    CTGAGCAACA TACGATCGCA GTGCATTTCA CATGCTGCTT TGGTGCTACA GGGATCCTTA
    ACGCAACCAA GGTCATTGCA GCAGCAGTCT TTGCTGGTAC CATATATGCT GTGTAGGAAT
    CTCCCATTTG GCTTCATCCA AGAACTGGTG AGAACAACTT ACCAAGATGA AGAGGTGTTC
    AAACAGATCT TTATCCCCAT CCTGCAAGGC CTGGCTGTAG CTTCCAAAGA GTGCTCCCTT
    GATAGTGACA ATTTTAAGTA CCCCCTTATG GCATTATGTG AACTTTGTGA AATCAAGTTT
    GGAAAAACAC ACCCCATGTG CAGTTTGGTT GTCTCTTTGC CCTTGTGGCT GCCTAAATCT
    TTAAGCACGG GTGCAGGAAG AGAGCTACAG AGACTTTCCT ATCTTGGAGC CTTCTTCAGT
    CTCTCTGTCT TTGCTGAAGA TGACAACAAA GTTGTTGAAA AGTACTTCTC AGGACCTGCC
    ATCACTCTTG AAAACACCCG AGTTGTTAGC CAGTCCTTGC AGCATTACCT GGAACTAGCG
    AGGCAAGAGC TCTTCAAGAT CCTGCATAGT ATTTTACTGA ATGGTGAAAC TCGAGAGGCA
    GCTCTCAGTT ACATGGCAGC TGTTGTCAAC GCCAACATGA AAAAGGCACA AATGCAGACA
    GATGATCGTT TGGTGTCTAC AGATGGTTTT ATGCTCAACT TCCTATGGGT GCTGCAGCAA
    CTAAGTATGA AGATCAAGCT GGAAACGGTT GATCCCATGT ACATATTTCA TCCCAGGTGT
    CGTATTGACC TCCCGACAGA TGAGACAAGA GTGAAAGCAA CAATGGAGGA TGTTACAGCC
    TGGATTGCTG AGCTTTACAG GGATCCGTCT CCATTTTCTG ATCCAAAATT CCCAACAGAA
    TGTTTCTTCT TAACCCTGCA TGCCCATCAT CTCTCCATCC TGCCGAGCTG CCGTCGGTAC
    ATACGTAGAC TGCGAGCCAT CAGGGAACTC AACAGGACTG TTGAAGATCT GAAAAACAAT
    GAAAGTCAAT GGAAGGATTC TCCTCTGGCT ACCAGACACA GAGAGATGCT AAAACGTTGC
    AAGACACAAC TTAAGAAACT GGTGAGGTGC AAGGCTTGTG CAGATGCTGG TTTGCTGGAT
    GAGAACTTTC TCAGGAGATG TCTGAATTTC TATGGCATGG TGATTCAACT GATGCTTCGC
    ATCCTCGACC CCGCCTATCC CAACATAAAA TTGCCTTTAA CACTTGAAGT TCCGAAGGTA
    TTTGCAGCAT TGCCAGAGTT TTACGTGGAA GATGTTGCTG AATTTTTATT TTTTATTGTA
    CAATATGCTC CTCAGGTGCT TTATGAGCCC TGTACTCAGG ACATAGTGAT GTTCCTTGTT
    GTGATGCTGT GCAACCAGAA CTACATCCGA AATCCTTATT TAGTTGCTAA GCTGGTGGAG
    GTGATGTTCA TGACCAATCC AGCTGTTCAG CCTCGAACAC AGAAGTTCTT TGAAATGATT
    GAGAATCATC CTCTGTCCAC TAAATTGTTA GTCCCCTCCT TAATGAAATT TTACACAGAT
    GTTGAGCATA CTGGAGCAAC TAGTGAATTC TATGACAAGT TTACAATCCG CTACCACATC
    AGCACCATTT TCAAAAGCCT CTGGCAGAAC ATAGCTCACC ATGGTACATT TATGGAAGAG
    TTCAACTCTG GGAAGCAGTT TGTTCGCTAC ATCAACATGC TGATAAATGA CACAACTTTC
    TTGCTGGATG AGAGTCTGGA ATCCCTAAAA CGTATCCATG AAGTGCAAGA GGAGATGAAG
    AATAAAGAAC AATGGGACCT GCTGCCTAGG GATCAGCAGC AGGCTCGGCA GTCGCAGCTG
    GCTCAGGATG AGCGTGTCTC TCGTTCATAT CTTGCCCTGG CAACAGAAAC TGTTGATATG
    TTCCATATCC TTACCAAGCA GGTTCAAAAG CCTTTTCTCA GACCTGAACT TGGACCACGA
    TTGGCTGCTA TGTTGAACTT TAACTTGCAG CAACTATGTG GCCCGAAGTG CCGTGACCTA
    AAAGTTGAGA ACCCTGAGAA ATACGGGTTT GAACCAAAGA AGCTGTTGGA CCAGCTGACT
    GACATTTATC TACAGCTAGA CTGTGCTCGT TTTGCTAAAG CAATTGCTGA TGATCAGAGG
    TCCTACAGCA AAGAGCTCTT TGAGGAGGTC ATATCAAAGA TGAGAAAGGC TGGAATCAAG
    TCAACCATAG CAATAGAAAA ATTCAAACTG CTGGCAGAGA AAGTGGAAGA GATTGTTGCC
    AAGAATGCCC GTGCAGAAAT TGATTACAGC GATGCTCCGG ATGAATTCAG AGATCCACTC
    ATGGACACTC TGATGACTGA TCCTGTGAGG TTGCCATCTG GAACTATTAT GGACAGGTCA
    ATCATCCTGA GGCATCTGTT GAATTCTTCC ACTGATCCCT TCAATCGTCA GACACTGACA
    GAAAATATGC TGGAACCAGT GCCAGAACTT AAAGAGCAAA TCCAGGCCTG GATGAGAGAC
    AAGCAGAATG CTGACCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004947510.1
    CDS108..4148
    Translation

    Target ORF information:

    RefSeq Version XM_004947453.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ubiquitination factor E4B (UBE4B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004947453.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGAGC TGAGCGCGGA CGAGATCCGT CGAAGGCGCC TTGCCCGGCT GGCAGGGGGA 
    CCATCGTCCC AGCCCAGCAC ACCGCTCACA TCGCCGCAGA GAGAGACCCC ACCTGGGCCA
    CCTGCAGCAG CTCCGACCCC GGGGCCATCC CATAGCCTCG GCCTAAATGT CCACAGCATG
    ACCCCAGCTA CCTCTCCGAT TGGTGCATCA GGAGTAGCCC ACCGAAGCCA GAGCAGCGAA
    GGGGTGAGTT CGCTCAGCAG TTCTCCATCA AACAGCCTTG AAACACAATC CCAGTCTCTC
    TCTCGGTCTC AGAGTATGGA TATTGATAGT GTCTCCTGTG AAAAAAGTAT GTCCCAGGTA
    GATGTGGATT CAGGAATTGA GAACATGGAA GTGGATGAGA ATGATCGCAG GGAGAAAAGG
    AGTCTGACTG ATAAGGAGCC TCTGTCTGGG TCTGAGGTGT CCGAGGAGCA AGCTTTACAG
    CTGGTCTGTA AAATCTTCAG AGTCTCTTGG AAGGACCGAG ACAGAGATGT TATCTTCCTG
    AATTCTCTCT CTGCTCAGTT TAAGCAGAAC CCAAAAGAAG TGTTTTCGGA TTTTAAGGAC
    TTGATCGGAC AGATCTTGAT GGAGGTGCTG ATGATGTCCA CCCAGTCAAG GGATGAAAAC
    CCATTTGCCA GTCTGACAGC TACGTCACAG CCAATTGCTG CAACTGCCCG GTCTCCTGAC
    AGAAGCCTGA TCTTAAACAC GGGCTCCAAC CCAGGAACCA GCCCTATGTT CTGTAATGTG
    GGCTCCTTTG GTTCCAGTTC GTTATCCAGT CTCTATGGGA CTAGTCCAGC TCCTACTACC
    AATTTCACAA GCTATGTACC AATGACTGGT TCATCTCTTA CCCCACCAGC CAGTTCAGTT
    GCCACCACAT CTGTGCCTTC CATTACTGTC AGCTCTCACC TCACAGCAAC CAGCCCTTCT
    TGGACCCCGC CTTCCTCTCC GAGGTACCGG CCATATACTG TTGCCCATTC TGGAGGCTTT
    TCAGCTTCTT CTATCCCACG TTCCTTGAAT ATTTCCATCC CAGCTAGTTC AAGTCCTCCA
    GTCATGGCTG CTAGCCCTCC AGCCATGCTG CTCACTGCAC CCAGACAGAG ACCCACGACT
    ATGGGTCCGC CTTTGTTTAC TTCACCCAGT ACCTTGCGCA GGCGTTCATC TTTACTGAAC
    AGGATTCCTT CTAGTATATA CGACAACCCT TTCTCCCTCC TCTTCCTTGG CGTTTCTGAT
    GTGTCTGAGG ACAGTAGTGA CGAAGAAAAT GAAGATGATG ATTTTTCTTG TGTCCAGTTT
    GGGTCCAGTG TGGGAGGCTC TGGCAGCTCA AGTGTTTGCG ATTCCTGCAG TGACCACTTC
    ACCATTGAAA ACTGTAAGGA GACAGAGATG CTGAACTACC TCATTGAGTG TTTTGACCGG
    GTTGGAATAG AGGAGCGAAA AGCACCAAAG ATGTGTAGCC AGCCAACAGT GAGTCAGTTA
    CTGAGCAACA TACGATCGCA GTGCATTTCA CATGCTGCTT TGGTGCTACA GGGATCCTTA
    ACGCAACCAA GGTCATTGCA GCAGCAGTCT TTGCTGGTAC CATATATGCT GTGTAGGAAT
    CTCCCATTTG GCTTCATCCA AGAACTGGTG AGAACAACTT ACCAAGATGA AGAGGTGTTC
    AAACAGATCT TTATCCCCAT CCTGCAAGGC CTGGCTGTAG CTTCCAAAGA GTGCTCCCTT
    GATAGTGACA ATTTTAAGTA CCCCCTTATG GCATTATGTG AACTTTGTGA AATCAAGTTT
    GGAAAAACAC ACCCCATGTG CAGTTTGGTT GTCTCTTTGC CCTTGTGGCT GCCTAAATCT
    TTAAGCACGG GTGCAGGAAG AGAGCTACAG AGACTTTCCT ATCTTGGAGC CTTCTTCAGT
    CTCTCTGTCT TTGCTGAAGA TGACAACAAA GTTGTTGAAA AGTACTTCTC AGGACCTGCC
    ATCACTCTTG AAAACACCCG AGTTGTTAGC CAGTCCTTGC AGCATTACCT GGAACTAGCG
    AGGCAAGAGC TCTTCAAGAT CCTGCATAGT ATTTTACTGA ATGGTGAAAC TCGAGAGGCA
    GCTCTCAGTT ACATGGCAGC TGTTGTCAAC GCCAACATGA AAAAGGCACA AATGCAGACA
    GATGATCGTT TGGTGTCTAC AGATGGTTTT ATGCTCAACT TCCTATGGGT GCTGCAGCAA
    CTAAGTATGA AGATCAAGCT GGAAACGGTT GATCCCATGT ACATATTTCA TCCCAGGTGT
    CGTATTGACC TCCCGACAGA TGAGACAAGA GTGAAAGCAA CAATGGAGGA TGTTACAGCC
    TGGATTGCTG AGCTTTACAG GGATCCGTCT CCATTTTCTG ATCCAAAATT CCCAACAGAA
    TGTTTCTTCT TAACCCTGCA TGCCCATCAT CTCTCCATCC TGCCGAGCTG CCGTCGGTAC
    ATACGTAGAC TGCGAGCCAT CAGGGAACTC AACAGGACTG TTGAAGATCT GAAAAACAAT
    GAAAGTCAAT GGAAGGATTC TCCTCTGGCT ACCAGACACA GAGAGATGCT AAAACGTTGC
    AAGACACAAC TTAAGAAACT GGTGAGGTGC AAGGCTTGTG CAGATGCTGG TTTGCTGGAT
    GAGAACTTTC TCAGGAGATG TCTGAATTTC TATGGCATGG TGATTCAACT GATGCTTCGC
    ATCCTCGACC CCGCCTATCC CAACATAAAA TTGCCTTTAA CACTTGAAGT TCCGAAGGTA
    TTTGCAGCAT TGCCAGAGTT TTACGTGGAA GATGTTGCTG AATTTTTATT TTTTATTGTA
    CAATATGCTC CTCAGGTGCT TTATGAGCCC TGTACTCAGG ACATAGTGAT GTTCCTTGTT
    GTGATGCTGT GCAACCAGAA CTACATCCGA AATCCTTATT TAGTTGCTAA GCTGGTGGAG
    GTGATGTTCA TGACCAATCC AGCTGTTCAG CCTCGAACAC AGAAGTTCTT TGAAATGATT
    GAGAATCATC CTCTGTCCAC TAAATTGTTA GTCCCCTCCT TAATGAAATT TTACACAGAT
    GTTGAGCATA CTGGAGCAAC TAGTGAATTC TATGACAAGT TTACAATCCG CTACCACATC
    AGCACCATTT TCAAAAGCCT CTGGCAGAAC ATAGCTCACC ATGGTACATT TATGGAAGAG
    TTCAACTCTG GGAAGCAGTT TGTTCGCTAC ATCAACATGC TGATAAATGA CACAACTTTC
    TTGCTGGATG AGAGTCTGGA ATCCCTAAAA CGTATCCATG AAGTGCAAGA GGAGATGAAG
    AATAAAGAAC AATGGGACCT GCTGCCTAGG GATCAGCAGC AGGCTCGGCA GTCGCAGCTG
    GCTCAGGATG AGCGTGTCTC TCGTTCATAT CTTGCCCTGG CAACAGAAAC TGTTGATATG
    TTCCATATCC TTACCAAGCA GGTTCAAAAG CCTTTTCTCA GACCTGAACT TGGACCACGA
    TTGGCTGCTA TGTTGAACTT TAACTTGCAG CAACTATGTG GCCCGAAGTG CCGTGACCTA
    AAAGTTGAGA ACCCTGAGAA ATACGGGTTT GAACCAAAGA AGCTGTTGGA CCAGCTGACT
    GACATTTATC TACAGCTAGA CTGTGCTCGT TTTGCTAAAG CAATTGCTGA TGATCAGAGG
    TCCTACAGCA AAGAGCTCTT TGAGGAGGTC ATATCAAAGA TGAGAAAGGC TGGAATCAAG
    TCAACCATAG CAATAGAAAA ATTCAAACTG CTGGCAGAGA AAGTGGAAGA GATTGTTGCC
    AAGAATGCCC GTGCAGAAAT TGATTACAGC GATGCTCCGG ATGAATTCAG AGATCCACTC
    ATGGACACTC TGATGACTGA TCCTGTGAGG TTGCCATCTG GAACTATTAT GGACAGGTCA
    ATCATCCTGA GGCATCTGTT GAATTCTTCC ACTGATCCCT TCAATCGTCA GACACTGACA
    GAAAATATGC TGGAACCAGT GCCAGAACTT AAAGAGCAAA TCCAGGCCTG GATGAGAGAC
    AAGCAGAATG CTGACCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa18254
    Clone ID Related Accession (Same CDS sequence) XM_003642511.3 , XM_003642511.4
    Accession Version XM_003642511.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product ubiquitin conjugation factor E4 B isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_003642511.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGGAGC TGAGCGCGGA CGAGATCCGT CGAAGGCGCC TTGCCCGGCT GGCAGGGGGA 
    CCATCGTCCC AGCCCAGCAC ACCGCTCACA TCGCCGCAGA GAGAGACCCC ACCTGGGCCA
    CCTGCAGCAG CTCCGACCCC GGGGCCATCC CATAGCCTCG GCCTAAATGT CCACAGCATG
    ACCCCAGCTA CCTCTCCGAT TGGTGCATCA GGAGTAGCCC ACCGAAGCCA GAGCAGCGAA
    GGGGTGAGTT CGCTCAGCAG TTCTCCATCA AACAGCCTTG AAACACAATC CCAGTCTCTC
    TCTCGGTCTC AGAGTATGGA TATTGATAGT GTCTCCTGTG AAAAAAGTAT GTCCCAGGTA
    GATGTGGATT CAGGAATTGA GAACATGGAA GTGGATGAGA ATGATCGCAG GGAGAAAAGG
    AGTCTGACTG ATAAGGAGCC TCTGTCTGGG TCTGAGGTGT CCGAGGAGCA AGCTTTACAG
    CTGGTCTGTA AAATCTTCAG AGTCTCTTGG AAGGACCGAG ACAGAGATGT TATCTTCCTG
    AATTCTCTCT CTGCTCAGTT TAAGCAGAAC CCAAAAGAAG TGTTTTCGGA TTTTAAGGAC
    TTGATCGGAC AGATCTTGAT GGAGGTGCTG ATGATGTCCA CCCAGTCAAG GGATGAAAAC
    CCATTTGCCA GTCTGACAGC TACGTCACAG CCAATTGCTG CAACTGCCCG GTCTCCTGAC
    AGAAGCCTGA TCTTAAACAC GGGCTCCAAC CCAGGAACCA GCCCTATGTT CTGTAATGTG
    GGCTCCTTTG GTTCCAGTTC GTTATCCAGT GTGGGAGGCT CTGGCAGCTC AAGTGTTTGC
    GATTCCTGCA GTGACCACTT CACCATTGAA AACTGTAAGG AGACAGAGAT GCTGAACTAC
    CTCATTGAGT GTTTTGACCG GGTTGGAATA GAGGAGCGAA AAGCACCAAA GATGTGTAGC
    CAGCCAACAG TGAGTCAGTT ACTGAGCAAC ATACGATCGC AGTGCATTTC ACATGCTGCT
    TTGGTGCTAC AGGGATCCTT AACGCAACCA AGGTCATTGC AGCAGCAGTC TTTGCTGGTA
    CCATATATGC TGTGTAGGAA TCTCCCATTT GGCTTCATCC AAGAACTGGT GAGAACAACT
    TACCAAGATG AAGAGGTGTT CAAACAGATC TTTATCCCCA TCCTGCAAGG CCTGGCTGTA
    GCTTCCAAAG AGTGCTCCCT TGATAGTGAC AATTTTAAGT ACCCCCTTAT GGCATTATGT
    GAACTTTGTG AAATCAAGTT TGGAAAAACA CACCCCATGT GCAGTTTGGT TGTCTCTTTG
    CCCTTGTGGC TGCCTAAATC TTTAAGCACG GGTGCAGGAA GAGAGCTACA GAGACTTTCC
    TATCTTGGAG CCTTCTTCAG TCTCTCTGTC TTTGCTGAAG ATGACAACAA AGTTGTTGAA
    AAGTACTTCT CAGGACCTGC CATCACTCTT GAAAACACCC GAGTTGTTAG CCAGTCCTTG
    CAGCATTACC TGGAACTAGC GAGGCAAGAG CTCTTCAAGA TCCTGCATAG TATTTTACTG
    AATGGTGAAA CTCGAGAGGC AGCTCTCAGT TACATGGCAG CTGTTGTCAA CGCCAACATG
    AAAAAGGCAC AAATGCAGAC AGATGATCGT TTGGTGTCTA CAGATGGTTT TATGCTCAAC
    TTCCTATGGG TGCTGCAGCA ACTAAGTATG AAGATCAAGC TGGAAACGGT TGATCCCATG
    TACATATTTC ATCCCAGGTG TCGTATTGAC CTCCCGACAG ATGAGACAAG AGTGAAAGCA
    ACAATGGAGG ATGTTACAGC CTGGATTGCT GAGCTTTACA GGGATCCGTC TCCATTTTCT
    GATCCAAAAT TCCCAACAGA ATGTTTCTTC TTAACCCTGC ATGCCCATCA TCTCTCCATC
    CTGCCGAGCT GCCGTCGGTA CATACGTAGA CTGCGAGCCA TCAGGGAACT CAACAGGACT
    GTTGAAGATC TGAAAAACAA TGAAAGTCAA TGGAAGGATT CTCCTCTGGC TACCAGACAC
    AGAGAGATGC TAAAACGTTG CAAGACACAA CTTAAGAAAC TGGTGAGGTG CAAGGCTTGT
    GCAGATGCTG GTTTGCTGGA TGAGAACTTT CTCAGGAGAT GTCTGAATTT CTATGGCATG
    GTGATTCAAC TGATGCTTCG CATCCTCGAC CCCGCCTATC CCAACATAAA ATTGCCTTTA
    ACACTTGAAG TTCCGAAGGT ATTTGCAGCA TTGCCAGAGT TTTACGTGGA AGATGTTGCT
    GAATTTTTAT TTTTTATTGT ACAATATGCT CCTCAGGTGC TTTATGAGCC CTGTACTCAG
    GACATAGTGA TGTTCCTTGT TGTGATGCTG TGCAACCAGA ACTACATCCG AAATCCTTAT
    TTAGTTGCTA AGCTGGTGGA GGTGATGTTC ATGACCAATC CAGCTGTTCA GCCTCGAACA
    CAGAAGTTCT TTGAAATGAT TGAGAATCAT CCTCTGTCCA CTAAATTGTT AGTCCCCTCC
    TTAATGAAAT TTTACACAGA TGTTGAGCAT ACTGGAGCAA CTAGTGAATT CTATGACAAG
    TTTACAATCC GCTACCACAT CAGCACCATT TTCAAAAGCC TCTGGCAGAA CATAGCTCAC
    CATGGTACAT TTATGGAAGA GTTCAACTCT GGGAAGCAGT TTGTTCGCTA CATCAACATG
    CTGATAAATG ACACAACTTT CTTGCTGGAT GAGAGTCTGG AATCCCTAAA ACGTATCCAT
    GAAGTGCAAG AGGAGATGAA GAATAAAGAA CAATGGGACC TGCTGCCTAG GGATCAGCAG
    CAGGCTCGGC AGTCGCAGCT GGCTCAGGAT GAGCGTGTCT CTCGTTCATA TCTTGCCCTG
    GCAACAGAAA CTGTTGATAT GTTCCATATC CTTACCAAGC AGGTTCAAAA GCCTTTTCTC
    AGACCTGAAC TTGGACCACG ATTGGCTGCT ATGTTGAACT TTAACTTGCA GCAACTATGT
    GGCCCGAAGT GCCGTGACCT AAAAGTTGAG AACCCTGAGA AATACGGGTT TGAACCAAAG
    AAGCTGTTGG ACCAGCTGAC TGACATTTAT CTACAGCTAG ACTGTGCTCG TTTTGCTAAA
    GCAATTGCTG ATGATCAGAG GTCCTACAGC AAAGAGCTCT TTGAGGAGGT CATATCAAAG
    ATGAGAAAGG CTGGAATCAA GTCAACCATA GCAATAGAAA AATTCAAACT GCTGGCAGAG
    AAAGTGGAAG AGATTGTTGC CAAGAATGCC CGTGCAGAAA TTGATTACAG CGATGCTCCG
    GATGAATTCA GAGATCCACT CATGGACACT CTGATGACTG ATCCTGTGAG GTTGCCATCT
    GGAACTATTA TGGACAGGTC AATCATCCTG AGGCATCTGT TGAATTCTTC CACTGATCCC
    TTCAATCGTC AGACACTGAC AGAAAATATG CTGGAACCAG TGCCAGAACT TAAAGAGCAA
    ATCCAGGCCT GGATGAGAGA CAAGCAGAAT GCTGACCATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003642559.1
    CDS102..3623
    Translation

    Target ORF information:

    RefSeq Version XM_003642511.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ubiquitination factor E4B (UBE4B), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003642511.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGGAGC TGAGCGCGGA CGAGATCCGT CGAAGGCGCC TTGCCCGGCT GGCAGGGGGA 
    CCATCGTCCC AGCCCAGCAC ACCGCTCACA TCGCCGCAGA GAGAGACCCC ACCTGGGCCA
    CCTGCAGCAG CTCCGACCCC GGGGCCATCC CATAGCCTCG GCCTAAATGT CCACAGCATG
    ACCCCAGCTA CCTCTCCGAT TGGTGCATCA GGAGTAGCCC ACCGAAGCCA GAGCAGCGAA
    GGGGTGAGTT CGCTCAGCAG TTCTCCATCA AACAGCCTTG AAACACAATC CCAGTCTCTC
    TCTCGGTCTC AGAGTATGGA TATTGATAGT GTCTCCTGTG AAAAAAGTAT GTCCCAGGTA
    GATGTGGATT CAGGAATTGA GAACATGGAA GTGGATGAGA ATGATCGCAG GGAGAAAAGG
    AGTCTGACTG ATAAGGAGCC TCTGTCTGGG TCTGAGGTGT CCGAGGAGCA AGCTTTACAG
    CTGGTCTGTA AAATCTTCAG AGTCTCTTGG AAGGACCGAG ACAGAGATGT TATCTTCCTG
    AATTCTCTCT CTGCTCAGTT TAAGCAGAAC CCAAAAGAAG TGTTTTCGGA TTTTAAGGAC
    TTGATCGGAC AGATCTTGAT GGAGGTGCTG ATGATGTCCA CCCAGTCAAG GGATGAAAAC
    CCATTTGCCA GTCTGACAGC TACGTCACAG CCAATTGCTG CAACTGCCCG GTCTCCTGAC
    AGAAGCCTGA TCTTAAACAC GGGCTCCAAC CCAGGAACCA GCCCTATGTT CTGTAATGTG
    GGCTCCTTTG GTTCCAGTTC GTTATCCAGT GTGGGAGGCT CTGGCAGCTC AAGTGTTTGC
    GATTCCTGCA GTGACCACTT CACCATTGAA AACTGTAAGG AGACAGAGAT GCTGAACTAC
    CTCATTGAGT GTTTTGACCG GGTTGGAATA GAGGAGCGAA AAGCACCAAA GATGTGTAGC
    CAGCCAACAG TGAGTCAGTT ACTGAGCAAC ATACGATCGC AGTGCATTTC ACATGCTGCT
    TTGGTGCTAC AGGGATCCTT AACGCAACCA AGGTCATTGC AGCAGCAGTC TTTGCTGGTA
    CCATATATGC TGTGTAGGAA TCTCCCATTT GGCTTCATCC AAGAACTGGT GAGAACAACT
    TACCAAGATG AAGAGGTGTT CAAACAGATC TTTATCCCCA TCCTGCAAGG CCTGGCTGTA
    GCTTCCAAAG AGTGCTCCCT TGATAGTGAC AATTTTAAGT ACCCCCTTAT GGCATTATGT
    GAACTTTGTG AAATCAAGTT TGGAAAAACA CACCCCATGT GCAGTTTGGT TGTCTCTTTG
    CCCTTGTGGC TGCCTAAATC TTTAAGCACG GGTGCAGGAA GAGAGCTACA GAGACTTTCC
    TATCTTGGAG CCTTCTTCAG TCTCTCTGTC TTTGCTGAAG ATGACAACAA AGTTGTTGAA
    AAGTACTTCT CAGGACCTGC CATCACTCTT GAAAACACCC GAGTTGTTAG CCAGTCCTTG
    CAGCATTACC TGGAACTAGC GAGGCAAGAG CTCTTCAAGA TCCTGCATAG TATTTTACTG
    AATGGTGAAA CTCGAGAGGC AGCTCTCAGT TACATGGCAG CTGTTGTCAA CGCCAACATG
    AAAAAGGCAC AAATGCAGAC AGATGATCGT TTGGTGTCTA CAGATGGTTT TATGCTCAAC
    TTCCTATGGG TGCTGCAGCA ACTAAGTATG AAGATCAAGC TGGAAACGGT TGATCCCATG
    TACATATTTC ATCCCAGGTG TCGTATTGAC CTCCCGACAG ATGAGACAAG AGTGAAAGCA
    ACAATGGAGG ATGTTACAGC CTGGATTGCT GAGCTTTACA GGGATCCGTC TCCATTTTCT
    GATCCAAAAT TCCCAACAGA ATGTTTCTTC TTAACCCTGC ATGCCCATCA TCTCTCCATC
    CTGCCGAGCT GCCGTCGGTA CATACGTAGA CTGCGAGCCA TCAGGGAACT CAACAGGACT
    GTTGAAGATC TGAAAAACAA TGAAAGTCAA TGGAAGGATT CTCCTCTGGC TACCAGACAC
    AGAGAGATGC TAAAACGTTG CAAGACACAA CTTAAGAAAC TGGTGAGGTG CAAGGCTTGT
    GCAGATGCTG GTTTGCTGGA TGAGAACTTT CTCAGGAGAT GTCTGAATTT CTATGGCATG
    GTGATTCAAC TGATGCTTCG CATCCTCGAC CCCGCCTATC CCAACATAAA ATTGCCTTTA
    ACACTTGAAG TTCCGAAGGT ATTTGCAGCA TTGCCAGAGT TTTACGTGGA AGATGTTGCT
    GAATTTTTAT TTTTTATTGT ACAATATGCT CCTCAGGTGC TTTATGAGCC CTGTACTCAG
    GACATAGTGA TGTTCCTTGT TGTGATGCTG TGCAACCAGA ACTACATCCG AAATCCTTAT
    TTAGTTGCTA AGCTGGTGGA GGTGATGTTC ATGACCAATC CAGCTGTTCA GCCTCGAACA
    CAGAAGTTCT TTGAAATGAT TGAGAATCAT CCTCTGTCCA CTAAATTGTT AGTCCCCTCC
    TTAATGAAAT TTTACACAGA TGTTGAGCAT ACTGGAGCAA CTAGTGAATT CTATGACAAG
    TTTACAATCC GCTACCACAT CAGCACCATT TTCAAAAGCC TCTGGCAGAA CATAGCTCAC
    CATGGTACAT TTATGGAAGA GTTCAACTCT GGGAAGCAGT TTGTTCGCTA CATCAACATG
    CTGATAAATG ACACAACTTT CTTGCTGGAT GAGAGTCTGG AATCCCTAAA ACGTATCCAT
    GAAGTGCAAG AGGAGATGAA GAATAAAGAA CAATGGGACC TGCTGCCTAG GGATCAGCAG
    CAGGCTCGGC AGTCGCAGCT GGCTCAGGAT GAGCGTGTCT CTCGTTCATA TCTTGCCCTG
    GCAACAGAAA CTGTTGATAT GTTCCATATC CTTACCAAGC AGGTTCAAAA GCCTTTTCTC
    AGACCTGAAC TTGGACCACG ATTGGCTGCT ATGTTGAACT TTAACTTGCA GCAACTATGT
    GGCCCGAAGT GCCGTGACCT AAAAGTTGAG AACCCTGAGA AATACGGGTT TGAACCAAAG
    AAGCTGTTGG ACCAGCTGAC TGACATTTAT CTACAGCTAG ACTGTGCTCG TTTTGCTAAA
    GCAATTGCTG ATGATCAGAG GTCCTACAGC AAAGAGCTCT TTGAGGAGGT CATATCAAAG
    ATGAGAAAGG CTGGAATCAA GTCAACCATA GCAATAGAAA AATTCAAACT GCTGGCAGAG
    AAAGTGGAAG AGATTGTTGC CAAGAATGCC CGTGCAGAAA TTGATTACAG CGATGCTCCG
    GATGAATTCA GAGATCCACT CATGGACACT CTGATGACTG ATCCTGTGAG GTTGCCATCT
    GGAACTATTA TGGACAGGTC AATCATCCTG AGGCATCTGT TGAATTCTTC CACTGATCCC
    TTCAATCGTC AGACACTGAC AGAAAATATG CTGGAACCAG TGCCAGAACT TAAAGAGCAA
    ATCCAGGCCT GGATGAGAGA CAAGCAGAAT GCTGACCATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa18254
    Clone ID Related Accession (Same CDS sequence) XM_003642511.3 , XM_003642511.4
    Accession Version XM_003642511.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ubiquitin conjugation factor E4 B isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006108.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_003642511.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGGAGC TGAGCGCGGA CGAGATCCGT CGAAGGCGCC TTGCCCGGCT GGCAGGGGGA 
    CCATCGTCCC AGCCCAGCAC ACCGCTCACA TCGCCGCAGA GAGAGACCCC ACCTGGGCCA
    CCTGCAGCAG CTCCGACCCC GGGGCCATCC CATAGCCTCG GCCTAAATGT CCACAGCATG
    ACCCCAGCTA CCTCTCCGAT TGGTGCATCA GGAGTAGCCC ACCGAAGCCA GAGCAGCGAA
    GGGGTGAGTT CGCTCAGCAG TTCTCCATCA AACAGCCTTG AAACACAATC CCAGTCTCTC
    TCTCGGTCTC AGAGTATGGA TATTGATAGT GTCTCCTGTG AAAAAAGTAT GTCCCAGGTA
    GATGTGGATT CAGGAATTGA GAACATGGAA GTGGATGAGA ATGATCGCAG GGAGAAAAGG
    AGTCTGACTG ATAAGGAGCC TCTGTCTGGG TCTGAGGTGT CCGAGGAGCA AGCTTTACAG
    CTGGTCTGTA AAATCTTCAG AGTCTCTTGG AAGGACCGAG ACAGAGATGT TATCTTCCTG
    AATTCTCTCT CTGCTCAGTT TAAGCAGAAC CCAAAAGAAG TGTTTTCGGA TTTTAAGGAC
    TTGATCGGAC AGATCTTGAT GGAGGTGCTG ATGATGTCCA CCCAGTCAAG GGATGAAAAC
    CCATTTGCCA GTCTGACAGC TACGTCACAG CCAATTGCTG CAACTGCCCG GTCTCCTGAC
    AGAAGCCTGA TCTTAAACAC GGGCTCCAAC CCAGGAACCA GCCCTATGTT CTGTAATGTG
    GGCTCCTTTG GTTCCAGTTC GTTATCCAGT GTGGGAGGCT CTGGCAGCTC AAGTGTTTGC
    GATTCCTGCA GTGACCACTT CACCATTGAA AACTGTAAGG AGACAGAGAT GCTGAACTAC
    CTCATTGAGT GTTTTGACCG GGTTGGAATA GAGGAGCGAA AAGCACCAAA GATGTGTAGC
    CAGCCAACAG TGAGTCAGTT ACTGAGCAAC ATACGATCGC AGTGCATTTC ACATGCTGCT
    TTGGTGCTAC AGGGATCCTT AACGCAACCA AGGTCATTGC AGCAGCAGTC TTTGCTGGTA
    CCATATATGC TGTGTAGGAA TCTCCCATTT GGCTTCATCC AAGAACTGGT GAGAACAACT
    TACCAAGATG AAGAGGTGTT CAAACAGATC TTTATCCCCA TCCTGCAAGG CCTGGCTGTA
    GCTTCCAAAG AGTGCTCCCT TGATAGTGAC AATTTTAAGT ACCCCCTTAT GGCATTATGT
    GAACTTTGTG AAATCAAGTT TGGAAAAACA CACCCCATGT GCAGTTTGGT TGTCTCTTTG
    CCCTTGTGGC TGCCTAAATC TTTAAGCACG GGTGCAGGAA GAGAGCTACA GAGACTTTCC
    TATCTTGGAG CCTTCTTCAG TCTCTCTGTC TTTGCTGAAG ATGACAACAA AGTTGTTGAA
    AAGTACTTCT CAGGACCTGC CATCACTCTT GAAAACACCC GAGTTGTTAG CCAGTCCTTG
    CAGCATTACC TGGAACTAGC GAGGCAAGAG CTCTTCAAGA TCCTGCATAG TATTTTACTG
    AATGGTGAAA CTCGAGAGGC AGCTCTCAGT TACATGGCAG CTGTTGTCAA CGCCAACATG
    AAAAAGGCAC AAATGCAGAC AGATGATCGT TTGGTGTCTA CAGATGGTTT TATGCTCAAC
    TTCCTATGGG TGCTGCAGCA ACTAAGTATG AAGATCAAGC TGGAAACGGT TGATCCCATG
    TACATATTTC ATCCCAGGTG TCGTATTGAC CTCCCGACAG ATGAGACAAG AGTGAAAGCA
    ACAATGGAGG ATGTTACAGC CTGGATTGCT GAGCTTTACA GGGATCCGTC TCCATTTTCT
    GATCCAAAAT TCCCAACAGA ATGTTTCTTC TTAACCCTGC ATGCCCATCA TCTCTCCATC
    CTGCCGAGCT GCCGTCGGTA CATACGTAGA CTGCGAGCCA TCAGGGAACT CAACAGGACT
    GTTGAAGATC TGAAAAACAA TGAAAGTCAA TGGAAGGATT CTCCTCTGGC TACCAGACAC
    AGAGAGATGC TAAAACGTTG CAAGACACAA CTTAAGAAAC TGGTGAGGTG CAAGGCTTGT
    GCAGATGCTG GTTTGCTGGA TGAGAACTTT CTCAGGAGAT GTCTGAATTT CTATGGCATG
    GTGATTCAAC TGATGCTTCG CATCCTCGAC CCCGCCTATC CCAACATAAA ATTGCCTTTA
    ACACTTGAAG TTCCGAAGGT ATTTGCAGCA TTGCCAGAGT TTTACGTGGA AGATGTTGCT
    GAATTTTTAT TTTTTATTGT ACAATATGCT CCTCAGGTGC TTTATGAGCC CTGTACTCAG
    GACATAGTGA TGTTCCTTGT TGTGATGCTG TGCAACCAGA ACTACATCCG AAATCCTTAT
    TTAGTTGCTA AGCTGGTGGA GGTGATGTTC ATGACCAATC CAGCTGTTCA GCCTCGAACA
    CAGAAGTTCT TTGAAATGAT TGAGAATCAT CCTCTGTCCA CTAAATTGTT AGTCCCCTCC
    TTAATGAAAT TTTACACAGA TGTTGAGCAT ACTGGAGCAA CTAGTGAATT CTATGACAAG
    TTTACAATCC GCTACCACAT CAGCACCATT TTCAAAAGCC TCTGGCAGAA CATAGCTCAC
    CATGGTACAT TTATGGAAGA GTTCAACTCT GGGAAGCAGT TTGTTCGCTA CATCAACATG
    CTGATAAATG ACACAACTTT CTTGCTGGAT GAGAGTCTGG AATCCCTAAA ACGTATCCAT
    GAAGTGCAAG AGGAGATGAA GAATAAAGAA CAATGGGACC TGCTGCCTAG GGATCAGCAG
    CAGGCTCGGC AGTCGCAGCT GGCTCAGGAT GAGCGTGTCT CTCGTTCATA TCTTGCCCTG
    GCAACAGAAA CTGTTGATAT GTTCCATATC CTTACCAAGC AGGTTCAAAA GCCTTTTCTC
    AGACCTGAAC TTGGACCACG ATTGGCTGCT ATGTTGAACT TTAACTTGCA GCAACTATGT
    GGCCCGAAGT GCCGTGACCT AAAAGTTGAG AACCCTGAGA AATACGGGTT TGAACCAAAG
    AAGCTGTTGG ACCAGCTGAC TGACATTTAT CTACAGCTAG ACTGTGCTCG TTTTGCTAAA
    GCAATTGCTG ATGATCAGAG GTCCTACAGC AAAGAGCTCT TTGAGGAGGT CATATCAAAG
    ATGAGAAAGG CTGGAATCAA GTCAACCATA GCAATAGAAA AATTCAAACT GCTGGCAGAG
    AAAGTGGAAG AGATTGTTGC CAAGAATGCC CGTGCAGAAA TTGATTACAG CGATGCTCCG
    GATGAATTCA GAGATCCACT CATGGACACT CTGATGACTG ATCCTGTGAG GTTGCCATCT
    GGAACTATTA TGGACAGGTC AATCATCCTG AGGCATCTGT TGAATTCTTC CACTGATCCC
    TTCAATCGTC AGACACTGAC AGAAAATATG CTGGAACCAG TGCCAGAACT TAAAGAGCAA
    ATCCAGGCCT GGATGAGAGA CAAGCAGAAT GCTGACCATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003642559.1
    CDS117..3638
    Translation

    Target ORF information:

    RefSeq Version XM_003642511.4
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ubiquitination factor E4B (UBE4B), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003642511.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGGAGC TGAGCGCGGA CGAGATCCGT CGAAGGCGCC TTGCCCGGCT GGCAGGGGGA 
    CCATCGTCCC AGCCCAGCAC ACCGCTCACA TCGCCGCAGA GAGAGACCCC ACCTGGGCCA
    CCTGCAGCAG CTCCGACCCC GGGGCCATCC CATAGCCTCG GCCTAAATGT CCACAGCATG
    ACCCCAGCTA CCTCTCCGAT TGGTGCATCA GGAGTAGCCC ACCGAAGCCA GAGCAGCGAA
    GGGGTGAGTT CGCTCAGCAG TTCTCCATCA AACAGCCTTG AAACACAATC CCAGTCTCTC
    TCTCGGTCTC AGAGTATGGA TATTGATAGT GTCTCCTGTG AAAAAAGTAT GTCCCAGGTA
    GATGTGGATT CAGGAATTGA GAACATGGAA GTGGATGAGA ATGATCGCAG GGAGAAAAGG
    AGTCTGACTG ATAAGGAGCC TCTGTCTGGG TCTGAGGTGT CCGAGGAGCA AGCTTTACAG
    CTGGTCTGTA AAATCTTCAG AGTCTCTTGG AAGGACCGAG ACAGAGATGT TATCTTCCTG
    AATTCTCTCT CTGCTCAGTT TAAGCAGAAC CCAAAAGAAG TGTTTTCGGA TTTTAAGGAC
    TTGATCGGAC AGATCTTGAT GGAGGTGCTG ATGATGTCCA CCCAGTCAAG GGATGAAAAC
    CCATTTGCCA GTCTGACAGC TACGTCACAG CCAATTGCTG CAACTGCCCG GTCTCCTGAC
    AGAAGCCTGA TCTTAAACAC GGGCTCCAAC CCAGGAACCA GCCCTATGTT CTGTAATGTG
    GGCTCCTTTG GTTCCAGTTC GTTATCCAGT GTGGGAGGCT CTGGCAGCTC AAGTGTTTGC
    GATTCCTGCA GTGACCACTT CACCATTGAA AACTGTAAGG AGACAGAGAT GCTGAACTAC
    CTCATTGAGT GTTTTGACCG GGTTGGAATA GAGGAGCGAA AAGCACCAAA GATGTGTAGC
    CAGCCAACAG TGAGTCAGTT ACTGAGCAAC ATACGATCGC AGTGCATTTC ACATGCTGCT
    TTGGTGCTAC AGGGATCCTT AACGCAACCA AGGTCATTGC AGCAGCAGTC TTTGCTGGTA
    CCATATATGC TGTGTAGGAA TCTCCCATTT GGCTTCATCC AAGAACTGGT GAGAACAACT
    TACCAAGATG AAGAGGTGTT CAAACAGATC TTTATCCCCA TCCTGCAAGG CCTGGCTGTA
    GCTTCCAAAG AGTGCTCCCT TGATAGTGAC AATTTTAAGT ACCCCCTTAT GGCATTATGT
    GAACTTTGTG AAATCAAGTT TGGAAAAACA CACCCCATGT GCAGTTTGGT TGTCTCTTTG
    CCCTTGTGGC TGCCTAAATC TTTAAGCACG GGTGCAGGAA GAGAGCTACA GAGACTTTCC
    TATCTTGGAG CCTTCTTCAG TCTCTCTGTC TTTGCTGAAG ATGACAACAA AGTTGTTGAA
    AAGTACTTCT CAGGACCTGC CATCACTCTT GAAAACACCC GAGTTGTTAG CCAGTCCTTG
    CAGCATTACC TGGAACTAGC GAGGCAAGAG CTCTTCAAGA TCCTGCATAG TATTTTACTG
    AATGGTGAAA CTCGAGAGGC AGCTCTCAGT TACATGGCAG CTGTTGTCAA CGCCAACATG
    AAAAAGGCAC AAATGCAGAC AGATGATCGT TTGGTGTCTA CAGATGGTTT TATGCTCAAC
    TTCCTATGGG TGCTGCAGCA ACTAAGTATG AAGATCAAGC TGGAAACGGT TGATCCCATG
    TACATATTTC ATCCCAGGTG TCGTATTGAC CTCCCGACAG ATGAGACAAG AGTGAAAGCA
    ACAATGGAGG ATGTTACAGC CTGGATTGCT GAGCTTTACA GGGATCCGTC TCCATTTTCT
    GATCCAAAAT TCCCAACAGA ATGTTTCTTC TTAACCCTGC ATGCCCATCA TCTCTCCATC
    CTGCCGAGCT GCCGTCGGTA CATACGTAGA CTGCGAGCCA TCAGGGAACT CAACAGGACT
    GTTGAAGATC TGAAAAACAA TGAAAGTCAA TGGAAGGATT CTCCTCTGGC TACCAGACAC
    AGAGAGATGC TAAAACGTTG CAAGACACAA CTTAAGAAAC TGGTGAGGTG CAAGGCTTGT
    GCAGATGCTG GTTTGCTGGA TGAGAACTTT CTCAGGAGAT GTCTGAATTT CTATGGCATG
    GTGATTCAAC TGATGCTTCG CATCCTCGAC CCCGCCTATC CCAACATAAA ATTGCCTTTA
    ACACTTGAAG TTCCGAAGGT ATTTGCAGCA TTGCCAGAGT TTTACGTGGA AGATGTTGCT
    GAATTTTTAT TTTTTATTGT ACAATATGCT CCTCAGGTGC TTTATGAGCC CTGTACTCAG
    GACATAGTGA TGTTCCTTGT TGTGATGCTG TGCAACCAGA ACTACATCCG AAATCCTTAT
    TTAGTTGCTA AGCTGGTGGA GGTGATGTTC ATGACCAATC CAGCTGTTCA GCCTCGAACA
    CAGAAGTTCT TTGAAATGAT TGAGAATCAT CCTCTGTCCA CTAAATTGTT AGTCCCCTCC
    TTAATGAAAT TTTACACAGA TGTTGAGCAT ACTGGAGCAA CTAGTGAATT CTATGACAAG
    TTTACAATCC GCTACCACAT CAGCACCATT TTCAAAAGCC TCTGGCAGAA CATAGCTCAC
    CATGGTACAT TTATGGAAGA GTTCAACTCT GGGAAGCAGT TTGTTCGCTA CATCAACATG
    CTGATAAATG ACACAACTTT CTTGCTGGAT GAGAGTCTGG AATCCCTAAA ACGTATCCAT
    GAAGTGCAAG AGGAGATGAA GAATAAAGAA CAATGGGACC TGCTGCCTAG GGATCAGCAG
    CAGGCTCGGC AGTCGCAGCT GGCTCAGGAT GAGCGTGTCT CTCGTTCATA TCTTGCCCTG
    GCAACAGAAA CTGTTGATAT GTTCCATATC CTTACCAAGC AGGTTCAAAA GCCTTTTCTC
    AGACCTGAAC TTGGACCACG ATTGGCTGCT ATGTTGAACT TTAACTTGCA GCAACTATGT
    GGCCCGAAGT GCCGTGACCT AAAAGTTGAG AACCCTGAGA AATACGGGTT TGAACCAAAG
    AAGCTGTTGG ACCAGCTGAC TGACATTTAT CTACAGCTAG ACTGTGCTCG TTTTGCTAAA
    GCAATTGCTG ATGATCAGAG GTCCTACAGC AAAGAGCTCT TTGAGGAGGT CATATCAAAG
    ATGAGAAAGG CTGGAATCAA GTCAACCATA GCAATAGAAA AATTCAAACT GCTGGCAGAG
    AAAGTGGAAG AGATTGTTGC CAAGAATGCC CGTGCAGAAA TTGATTACAG CGATGCTCCG
    GATGAATTCA GAGATCCACT CATGGACACT CTGATGACTG ATCCTGTGAG GTTGCCATCT
    GGAACTATTA TGGACAGGTC AATCATCCTG AGGCATCTGT TGAATTCTTC CACTGATCCC
    TTCAATCGTC AGACACTGAC AGAAAATATG CTGGAACCAG TGCCAGAACT TAAAGAGCAA
    ATCCAGGCCT GGATGAGAGA CAAGCAGAAT GCTGACCATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45208
    Clone ID Related Accession (Same CDS sequence) XM_015297149.2 , XM_015297149.1
    Accession Version XM_015297149.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3699bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ubiquitin conjugation factor E4 B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006108.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015297149.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGGAGC TGAGCGCGGA CGAGATCCGT CGAAGGCGCC TTGCCCGGCT GGCAGGGGGA 
    CCATCGTCCC AGCCCAGCAC ACCGCTCACA TCGCCGCAGA GAGAGACCCC ACCTGGGCCA
    CCTGCAGCAG CTCCGACCCC GGGGCCATCC CATAGCCTCG GCCTAAATGT CCACAGCATG
    ACCCCAGCTA CCTCTCCGAT TGGTGCATCA GGAGTAGCCC ACCGAAGCCA GAGCAGCGAA
    GGGGTGAGTT CGCTCAGCAG TTCTCCATCA AACAGCCTTG AAACACAATC CCAGTCTCTC
    TCTCGGTCTC AGAGTATGGA TATTGATAGT GTCTCCTGTG AAAAAAGTAT GTCCCAGGTA
    GATGTGGATT CAGGAATTGA GAACATGGAA GTGGATGAGA ATGATCGCAG GGAGAAAAGG
    AGTCTGACTG ATAAGGAGGA GCCTCTGTCT GGGTCTGAGG TGTCCGAGGA GCAAGCTTTA
    CAGCTGGTCT GTAAAATCTT CAGAGTCTCT TGGAAGGACC GAGACAGAGA TGTTATCTTC
    CTGAATTCTC TCTCTGCTCA GTTTAAGCAG AACCCAAAAG AAGTGTTTTC GGATTTTAAG
    GACTTGATCG GACAGATCTT GATGGAGGTG CTGATGATGT CCACCCAGTC AAGGGATGAA
    AACCCATTTG CCAGTCTGAC AGCTACGTCA CAGCCAATTG CTGCAACTGC CCGGTCTCCT
    GACAGAAGCC TGATCTTAAA CACGGGCTCC AACCCAGGAA CCAGCCCTAT GTTCTGTAAT
    GTGGGCTCCT TTGGTTCCAG TTCGTTATCC AGTCTCTATG GGACTAGTCC AGCTCCTACT
    ACCAATTTCA CAAGCTATGT ACCAATGACT GGTTCATCTC TTACCCCACC AGCCAGTTCA
    GTTGCCACCA CATCTGTGCC TTCCATTACT GTCAGCTCTC ACCTCACAGC AACCAGCCCT
    TCTTGGACCC CGCCTTCCTC TCCGAGTGTG GGAGGCTCTG GCAGCTCAAG TGTTTGCGAT
    TCCTGCAGTG ACCACTTCAC CATTGAAAAC TGTAAGGAGA CAGAGATGCT GAACTACCTC
    ATTGAGTGTT TTGACCGGGT TGGAATAGAG GAGCGAAAAG CACCAAAGAT GTGTAGCCAG
    CCAACAGTGA GTCAGTTACT GAGCAACATA CGATCGCAGT GCATTTCACA TGCTGCTTTG
    GTGCTACAGG GATCCTTAAC GCAACCAAGG TCATTGCAGC AGCAGTCTTT GCTGGTACCA
    TATATGCTGT GTAGGAATCT CCCATTTGGC TTCATCCAAG AACTGGTGAG AACAACTTAC
    CAAGATGAAG AGGTGTTCAA ACAGATCTTT ATCCCCATCC TGCAAGGCCT GGCTGTAGCT
    TCCAAAGAGT GCTCCCTTGA TAGTGACAAT TTTAAGTACC CCCTTATGGC ATTATGTGAA
    CTTTGTGAAA TCAAGTTTGG AAAAACACAC CCCATGTGCA GTTTGGTTGT CTCTTTGCCC
    TTGTGGCTGC CTAAATCTTT AAGCACGGGT GCAGGAAGAG AGCTACAGAG ACTTTCCTAT
    CTTGGAGCCT TCTTCAGTCT CTCTGTCTTT GCTGAAGATG ACAACAAAGT TGTTGAAAAG
    TACTTCTCAG GACCTGCCAT CACTCTTGAA AACACCCGAG TTGTTAGCCA GTCCTTGCAG
    CATTACCTGG AACTAGCGAG GCAAGAGCTC TTCAAGATCC TGCATAGTAT TTTACTGAAT
    GGTGAAACTC GAGAGGCAGC TCTCAGTTAC ATGGCAGCTG TTGTCAACGC CAACATGAAA
    AAGGCACAAA TGCAGACAGA TGATCGTTTG GTGTCTACAG ATGGTTTTAT GCTCAACTTC
    CTATGGGTGC TGCAGCAACT AAGTATGAAG ATCAAGCTGG AAACGGTTGA TCCCATGTAC
    ATATTTCATC CCAGGTGTCG TATTGACCTC CCGACAGATG AGACAAGAGT GAAAGCAACA
    ATGGAGGATG TTACAGCCTG GATTGCTGAG CTTTACAGGG ATCCGTCTCC ATTTTCTGAT
    CCAAAATTCC CAACAGAATG TTTCTTCTTA ACCCTGCATG CCCATCATCT CTCCATCCTG
    CCGAGCTGCC GTCGGTACAT ACGTAGACTG CGAGCCATCA GGGAACTCAA CAGGACTGTT
    GAAGATCTGA AAAACAATGA AAGTCAATGG AAGGATTCTC CTCTGGCTAC CAGACACAGA
    GAGATGCTAA AACGTTGCAA GACACAACTT AAGAAACTGG TGAGGTGCAA GGCTTGTGCA
    GATGCTGGTT TGCTGGATGA GAACTTTCTC AGGAGATGTC TGAATTTCTA TGGCATGGTG
    ATTCAACTGA TGCTTCGCAT CCTCGACCCC GCCTATCCCA ACATAAAATT GCCTTTAACA
    CTTGAAGTTC CGAAGGTATT TGCAGCATTG CCAGAGTTTT ACGTGGAAGA TGTTGCTGAA
    TTTTTATTTT TTATTGTACA ATATGCTCCT CAGGTGCTTT ATGAGCCCTG TACTCAGGAC
    ATAGTGATGT TCCTTGTTGT GATGCTGTGC AACCAGAACT ACATCCGAAA TCCTTATTTA
    GTTGCTAAGC TGGTGGAGGT GATGTTCATG ACCAATCCAG CTGTTCAGCC TCGAACACAG
    AAGTTCTTTG AAATGATTGA GAATCATCCT CTGTCCACTA AATTGTTAGT CCCCTCCTTA
    ATGAAATTTT ACACAGATGT TGAGCATACT GGAGCAACTA GTGAATTCTA TGACAAGTTT
    ACAATCCGCT ACCACATCAG CACCATTTTC AAAAGCCTCT GGCAGAACAT AGCTCACCAT
    GGTACATTTA TGGAAGAGTT CAACTCTGGG AAGCAGTTTG TTCGCTACAT CAACATGCTG
    ATAAATGACA CAACTTTCTT GCTGGATGAG AGTCTGGAAT CCCTAAAACG TATCCATGAA
    GTGCAAGAGG AGATGAAGAA TAAAGAACAA TGGGACCTGC TGCCTAGGGA TCAGCAGCAG
    GCTCGGCAGT CGCAGCTGGC TCAGGATGAG CGTGTCTCTC GTTCATATCT TGCCCTGGCA
    ACAGAAACTG TTGATATGTT CCATATCCTT ACCAAGCAGG TTCAAAAGCC TTTTCTCAGA
    CCTGAACTTG GACCACGATT GGCTGCTATG TTGAACTTTA ACTTGCAGCA ACTATGTGGC
    CCGAAGTGCC GTGACCTAAA AGTTGAGAAC CCTGAGAAAT ACGGGTTTGA ACCAAAGAAG
    CTGTTGGACC AGCTGACTGA CATTTATCTA CAGCTAGACT GTGCTCGTTT TGCTAAAGCA
    ATTGCTGATG ATCAGAGGTC CTACAGCAAA GAGCTCTTTG AGGAGGTCAT ATCAAAGATG
    AGAAAGGCTG GAATCAAGTC AACCATAGCA ATAGAAAAAT TCAAACTGCT GGCAGAGAAA
    GTGGAAGAGA TTGTTGCCAA GAATGCCCGT GCAGAAATTG ATTACAGCGA TGCTCCGGAT
    GAATTCAGAG ATCCACTCAT GGACACTCTG ATGACTGATC CTGTGAGGTT GCCATCTGGA
    ACTATTATGG ACAGGTCAAT CATCCTGAGG CATCTGTTGA ATTCTTCCAC TGATCCCTTC
    AATCGTCAGA CACTGACAGA AAATATGCTG GAACCAGTGC CAGAACTTAA AGAGCAAATC
    CAGGCCTGGA TGAGAGACAA GCAGAATGCT GACCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015152635.1
    CDS117..3815
    Translation

    Target ORF information:

    RefSeq Version XM_015297149.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ubiquitination factor E4B (UBE4B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015297149.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGGAGC TGAGCGCGGA CGAGATCCGT CGAAGGCGCC TTGCCCGGCT GGCAGGGGGA 
    CCATCGTCCC AGCCCAGCAC ACCGCTCACA TCGCCGCAGA GAGAGACCCC ACCTGGGCCA
    CCTGCAGCAG CTCCGACCCC GGGGCCATCC CATAGCCTCG GCCTAAATGT CCACAGCATG
    ACCCCAGCTA CCTCTCCGAT TGGTGCATCA GGAGTAGCCC ACCGAAGCCA GAGCAGCGAA
    GGGGTGAGTT CGCTCAGCAG TTCTCCATCA AACAGCCTTG AAACACAATC CCAGTCTCTC
    TCTCGGTCTC AGAGTATGGA TATTGATAGT GTCTCCTGTG AAAAAAGTAT GTCCCAGGTA
    GATGTGGATT CAGGAATTGA GAACATGGAA GTGGATGAGA ATGATCGCAG GGAGAAAAGG
    AGTCTGACTG ATAAGGAGGA GCCTCTGTCT GGGTCTGAGG TGTCCGAGGA GCAAGCTTTA
    CAGCTGGTCT GTAAAATCTT CAGAGTCTCT TGGAAGGACC GAGACAGAGA TGTTATCTTC
    CTGAATTCTC TCTCTGCTCA GTTTAAGCAG AACCCAAAAG AAGTGTTTTC GGATTTTAAG
    GACTTGATCG GACAGATCTT GATGGAGGTG CTGATGATGT CCACCCAGTC AAGGGATGAA
    AACCCATTTG CCAGTCTGAC AGCTACGTCA CAGCCAATTG CTGCAACTGC CCGGTCTCCT
    GACAGAAGCC TGATCTTAAA CACGGGCTCC AACCCAGGAA CCAGCCCTAT GTTCTGTAAT
    GTGGGCTCCT TTGGTTCCAG TTCGTTATCC AGTCTCTATG GGACTAGTCC AGCTCCTACT
    ACCAATTTCA CAAGCTATGT ACCAATGACT GGTTCATCTC TTACCCCACC AGCCAGTTCA
    GTTGCCACCA CATCTGTGCC TTCCATTACT GTCAGCTCTC ACCTCACAGC AACCAGCCCT
    TCTTGGACCC CGCCTTCCTC TCCGAGTGTG GGAGGCTCTG GCAGCTCAAG TGTTTGCGAT
    TCCTGCAGTG ACCACTTCAC CATTGAAAAC TGTAAGGAGA CAGAGATGCT GAACTACCTC
    ATTGAGTGTT TTGACCGGGT TGGAATAGAG GAGCGAAAAG CACCAAAGAT GTGTAGCCAG
    CCAACAGTGA GTCAGTTACT GAGCAACATA CGATCGCAGT GCATTTCACA TGCTGCTTTG
    GTGCTACAGG GATCCTTAAC GCAACCAAGG TCATTGCAGC AGCAGTCTTT GCTGGTACCA
    TATATGCTGT GTAGGAATCT CCCATTTGGC TTCATCCAAG AACTGGTGAG AACAACTTAC
    CAAGATGAAG AGGTGTTCAA ACAGATCTTT ATCCCCATCC TGCAAGGCCT GGCTGTAGCT
    TCCAAAGAGT GCTCCCTTGA TAGTGACAAT TTTAAGTACC CCCTTATGGC ATTATGTGAA
    CTTTGTGAAA TCAAGTTTGG AAAAACACAC CCCATGTGCA GTTTGGTTGT CTCTTTGCCC
    TTGTGGCTGC CTAAATCTTT AAGCACGGGT GCAGGAAGAG AGCTACAGAG ACTTTCCTAT
    CTTGGAGCCT TCTTCAGTCT CTCTGTCTTT GCTGAAGATG ACAACAAAGT TGTTGAAAAG
    TACTTCTCAG GACCTGCCAT CACTCTTGAA AACACCCGAG TTGTTAGCCA GTCCTTGCAG
    CATTACCTGG AACTAGCGAG GCAAGAGCTC TTCAAGATCC TGCATAGTAT TTTACTGAAT
    GGTGAAACTC GAGAGGCAGC TCTCAGTTAC ATGGCAGCTG TTGTCAACGC CAACATGAAA
    AAGGCACAAA TGCAGACAGA TGATCGTTTG GTGTCTACAG ATGGTTTTAT GCTCAACTTC
    CTATGGGTGC TGCAGCAACT AAGTATGAAG ATCAAGCTGG AAACGGTTGA TCCCATGTAC
    ATATTTCATC CCAGGTGTCG TATTGACCTC CCGACAGATG AGACAAGAGT GAAAGCAACA
    ATGGAGGATG TTACAGCCTG GATTGCTGAG CTTTACAGGG ATCCGTCTCC ATTTTCTGAT
    CCAAAATTCC CAACAGAATG TTTCTTCTTA ACCCTGCATG CCCATCATCT CTCCATCCTG
    CCGAGCTGCC GTCGGTACAT ACGTAGACTG CGAGCCATCA GGGAACTCAA CAGGACTGTT
    GAAGATCTGA AAAACAATGA AAGTCAATGG AAGGATTCTC CTCTGGCTAC CAGACACAGA
    GAGATGCTAA AACGTTGCAA GACACAACTT AAGAAACTGG TGAGGTGCAA GGCTTGTGCA
    GATGCTGGTT TGCTGGATGA GAACTTTCTC AGGAGATGTC TGAATTTCTA TGGCATGGTG
    ATTCAACTGA TGCTTCGCAT CCTCGACCCC GCCTATCCCA ACATAAAATT GCCTTTAACA
    CTTGAAGTTC CGAAGGTATT TGCAGCATTG CCAGAGTTTT ACGTGGAAGA TGTTGCTGAA
    TTTTTATTTT TTATTGTACA ATATGCTCCT CAGGTGCTTT ATGAGCCCTG TACTCAGGAC
    ATAGTGATGT TCCTTGTTGT GATGCTGTGC AACCAGAACT ACATCCGAAA TCCTTATTTA
    GTTGCTAAGC TGGTGGAGGT GATGTTCATG ACCAATCCAG CTGTTCAGCC TCGAACACAG
    AAGTTCTTTG AAATGATTGA GAATCATCCT CTGTCCACTA AATTGTTAGT CCCCTCCTTA
    ATGAAATTTT ACACAGATGT TGAGCATACT GGAGCAACTA GTGAATTCTA TGACAAGTTT
    ACAATCCGCT ACCACATCAG CACCATTTTC AAAAGCCTCT GGCAGAACAT AGCTCACCAT
    GGTACATTTA TGGAAGAGTT CAACTCTGGG AAGCAGTTTG TTCGCTACAT CAACATGCTG
    ATAAATGACA CAACTTTCTT GCTGGATGAG AGTCTGGAAT CCCTAAAACG TATCCATGAA
    GTGCAAGAGG AGATGAAGAA TAAAGAACAA TGGGACCTGC TGCCTAGGGA TCAGCAGCAG
    GCTCGGCAGT CGCAGCTGGC TCAGGATGAG CGTGTCTCTC GTTCATATCT TGCCCTGGCA
    ACAGAAACTG TTGATATGTT CCATATCCTT ACCAAGCAGG TTCAAAAGCC TTTTCTCAGA
    CCTGAACTTG GACCACGATT GGCTGCTATG TTGAACTTTA ACTTGCAGCA ACTATGTGGC
    CCGAAGTGCC GTGACCTAAA AGTTGAGAAC CCTGAGAAAT ACGGGTTTGA ACCAAAGAAG
    CTGTTGGACC AGCTGACTGA CATTTATCTA CAGCTAGACT GTGCTCGTTT TGCTAAAGCA
    ATTGCTGATG ATCAGAGGTC CTACAGCAAA GAGCTCTTTG AGGAGGTCAT ATCAAAGATG
    AGAAAGGCTG GAATCAAGTC AACCATAGCA ATAGAAAAAT TCAAACTGCT GGCAGAGAAA
    GTGGAAGAGA TTGTTGCCAA GAATGCCCGT GCAGAAATTG ATTACAGCGA TGCTCCGGAT
    GAATTCAGAG ATCCACTCAT GGACACTCTG ATGACTGATC CTGTGAGGTT GCCATCTGGA
    ACTATTATGG ACAGGTCAAT CATCCTGAGG CATCTGTTGA ATTCTTCCAC TGATCCCTTC
    AATCGTCAGA CACTGACAGA AAATATGCTG GAACCAGTGC CAGAACTTAA AGAGCAAATC
    CAGGCCTGGA TGAGAGACAA GCAGAATGCT GACCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45208
    Clone ID Related Accession (Same CDS sequence) XM_015297149.2 , XM_015297149.1
    Accession Version XM_015297149.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3699bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product ubiquitin conjugation factor E4 B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGGAGC TGAGCGCGGA CGAGATCCGT CGAAGGCGCC TTGCCCGGCT GGCAGGGGGA 
    CCATCGTCCC AGCCCAGCAC ACCGCTCACA TCGCCGCAGA GAGAGACCCC ACCTGGGCCA
    CCTGCAGCAG CTCCGACCCC GGGGCCATCC CATAGCCTCG GCCTAAATGT CCACAGCATG
    ACCCCAGCTA CCTCTCCGAT TGGTGCATCA GGAGTAGCCC ACCGAAGCCA GAGCAGCGAA
    GGGGTGAGTT CGCTCAGCAG TTCTCCATCA AACAGCCTTG AAACACAATC CCAGTCTCTC
    TCTCGGTCTC AGAGTATGGA TATTGATAGT GTCTCCTGTG AAAAAAGTAT GTCCCAGGTA
    GATGTGGATT CAGGAATTGA GAACATGGAA GTGGATGAGA ATGATCGCAG GGAGAAAAGG
    AGTCTGACTG ATAAGGAGGA GCCTCTGTCT GGGTCTGAGG TGTCCGAGGA GCAAGCTTTA
    CAGCTGGTCT GTAAAATCTT CAGAGTCTCT TGGAAGGACC GAGACAGAGA TGTTATCTTC
    CTGAATTCTC TCTCTGCTCA GTTTAAGCAG AACCCAAAAG AAGTGTTTTC GGATTTTAAG
    GACTTGATCG GACAGATCTT GATGGAGGTG CTGATGATGT CCACCCAGTC AAGGGATGAA
    AACCCATTTG CCAGTCTGAC AGCTACGTCA CAGCCAATTG CTGCAACTGC CCGGTCTCCT
    GACAGAAGCC TGATCTTAAA CACGGGCTCC AACCCAGGAA CCAGCCCTAT GTTCTGTAAT
    GTGGGCTCCT TTGGTTCCAG TTCGTTATCC AGTCTCTATG GGACTAGTCC AGCTCCTACT
    ACCAATTTCA CAAGCTATGT ACCAATGACT GGTTCATCTC TTACCCCACC AGCCAGTTCA
    GTTGCCACCA CATCTGTGCC TTCCATTACT GTCAGCTCTC ACCTCACAGC AACCAGCCCT
    TCTTGGACCC CGCCTTCCTC TCCGAGTGTG GGAGGCTCTG GCAGCTCAAG TGTTTGCGAT
    TCCTGCAGTG ACCACTTCAC CATTGAAAAC TGTAAGGAGA CAGAGATGCT GAACTACCTC
    ATTGAGTGTT TTGACCGGGT TGGAATAGAG GAGCGAAAAG CACCAAAGAT GTGTAGCCAG
    CCAACAGTGA GTCAGTTACT GAGCAACATA CGATCGCAGT GCATTTCACA TGCTGCTTTG
    GTGCTACAGG GATCCTTAAC GCAACCAAGG TCATTGCAGC AGCAGTCTTT GCTGGTACCA
    TATATGCTGT GTAGGAATCT CCCATTTGGC TTCATCCAAG AACTGGTGAG AACAACTTAC
    CAAGATGAAG AGGTGTTCAA ACAGATCTTT ATCCCCATCC TGCAAGGCCT GGCTGTAGCT
    TCCAAAGAGT GCTCCCTTGA TAGTGACAAT TTTAAGTACC CCCTTATGGC ATTATGTGAA
    CTTTGTGAAA TCAAGTTTGG AAAAACACAC CCCATGTGCA GTTTGGTTGT CTCTTTGCCC
    TTGTGGCTGC CTAAATCTTT AAGCACGGGT GCAGGAAGAG AGCTACAGAG ACTTTCCTAT
    CTTGGAGCCT TCTTCAGTCT CTCTGTCTTT GCTGAAGATG ACAACAAAGT TGTTGAAAAG
    TACTTCTCAG GACCTGCCAT CACTCTTGAA AACACCCGAG TTGTTAGCCA GTCCTTGCAG
    CATTACCTGG AACTAGCGAG GCAAGAGCTC TTCAAGATCC TGCATAGTAT TTTACTGAAT
    GGTGAAACTC GAGAGGCAGC TCTCAGTTAC ATGGCAGCTG TTGTCAACGC CAACATGAAA
    AAGGCACAAA TGCAGACAGA TGATCGTTTG GTGTCTACAG ATGGTTTTAT GCTCAACTTC
    CTATGGGTGC TGCAGCAACT AAGTATGAAG ATCAAGCTGG AAACGGTTGA TCCCATGTAC
    ATATTTCATC CCAGGTGTCG TATTGACCTC CCGACAGATG AGACAAGAGT GAAAGCAACA
    ATGGAGGATG TTACAGCCTG GATTGCTGAG CTTTACAGGG ATCCGTCTCC ATTTTCTGAT
    CCAAAATTCC CAACAGAATG TTTCTTCTTA ACCCTGCATG CCCATCATCT CTCCATCCTG
    CCGAGCTGCC GTCGGTACAT ACGTAGACTG CGAGCCATCA GGGAACTCAA CAGGACTGTT
    GAAGATCTGA AAAACAATGA AAGTCAATGG AAGGATTCTC CTCTGGCTAC CAGACACAGA
    GAGATGCTAA AACGTTGCAA GACACAACTT AAGAAACTGG TGAGGTGCAA GGCTTGTGCA
    GATGCTGGTT TGCTGGATGA GAACTTTCTC AGGAGATGTC TGAATTTCTA TGGCATGGTG
    ATTCAACTGA TGCTTCGCAT CCTCGACCCC GCCTATCCCA ACATAAAATT GCCTTTAACA
    CTTGAAGTTC CGAAGGTATT TGCAGCATTG CCAGAGTTTT ACGTGGAAGA TGTTGCTGAA
    TTTTTATTTT TTATTGTACA ATATGCTCCT CAGGTGCTTT ATGAGCCCTG TACTCAGGAC
    ATAGTGATGT TCCTTGTTGT GATGCTGTGC AACCAGAACT ACATCCGAAA TCCTTATTTA
    GTTGCTAAGC TGGTGGAGGT GATGTTCATG ACCAATCCAG CTGTTCAGCC TCGAACACAG
    AAGTTCTTTG AAATGATTGA GAATCATCCT CTGTCCACTA AATTGTTAGT CCCCTCCTTA
    ATGAAATTTT ACACAGATGT TGAGCATACT GGAGCAACTA GTGAATTCTA TGACAAGTTT
    ACAATCCGCT ACCACATCAG CACCATTTTC AAAAGCCTCT GGCAGAACAT AGCTCACCAT
    GGTACATTTA TGGAAGAGTT CAACTCTGGG AAGCAGTTTG TTCGCTACAT CAACATGCTG
    ATAAATGACA CAACTTTCTT GCTGGATGAG AGTCTGGAAT CCCTAAAACG TATCCATGAA
    GTGCAAGAGG AGATGAAGAA TAAAGAACAA TGGGACCTGC TGCCTAGGGA TCAGCAGCAG
    GCTCGGCAGT CGCAGCTGGC TCAGGATGAG CGTGTCTCTC GTTCATATCT TGCCCTGGCA
    ACAGAAACTG TTGATATGTT CCATATCCTT ACCAAGCAGG TTCAAAAGCC TTTTCTCAGA
    CCTGAACTTG GACCACGATT GGCTGCTATG TTGAACTTTA ACTTGCAGCA ACTATGTGGC
    CCGAAGTGCC GTGACCTAAA AGTTGAGAAC CCTGAGAAAT ACGGGTTTGA ACCAAAGAAG
    CTGTTGGACC AGCTGACTGA CATTTATCTA CAGCTAGACT GTGCTCGTTT TGCTAAAGCA
    ATTGCTGATG ATCAGAGGTC CTACAGCAAA GAGCTCTTTG AGGAGGTCAT ATCAAAGATG
    AGAAAGGCTG GAATCAAGTC AACCATAGCA ATAGAAAAAT TCAAACTGCT GGCAGAGAAA
    GTGGAAGAGA TTGTTGCCAA GAATGCCCGT GCAGAAATTG ATTACAGCGA TGCTCCGGAT
    GAATTCAGAG ATCCACTCAT GGACACTCTG ATGACTGATC CTGTGAGGTT GCCATCTGGA
    ACTATTATGG ACAGGTCAAT CATCCTGAGG CATCTGTTGA ATTCTTCCAC TGATCCCTTC
    AATCGTCAGA CACTGACAGA AAATATGCTG GAACCAGTGC CAGAACTTAA AGAGCAAATC
    CAGGCCTGGA TGAGAGACAA GCAGAATGCT GACCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015152635.1
    CDS104..3802
    Translation

    Target ORF information:

    RefSeq Version XM_015297149.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ubiquitination factor E4B (UBE4B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015297149.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGGAGC TGAGCGCGGA CGAGATCCGT CGAAGGCGCC TTGCCCGGCT GGCAGGGGGA 
    CCATCGTCCC AGCCCAGCAC ACCGCTCACA TCGCCGCAGA GAGAGACCCC ACCTGGGCCA
    CCTGCAGCAG CTCCGACCCC GGGGCCATCC CATAGCCTCG GCCTAAATGT CCACAGCATG
    ACCCCAGCTA CCTCTCCGAT TGGTGCATCA GGAGTAGCCC ACCGAAGCCA GAGCAGCGAA
    GGGGTGAGTT CGCTCAGCAG TTCTCCATCA AACAGCCTTG AAACACAATC CCAGTCTCTC
    TCTCGGTCTC AGAGTATGGA TATTGATAGT GTCTCCTGTG AAAAAAGTAT GTCCCAGGTA
    GATGTGGATT CAGGAATTGA GAACATGGAA GTGGATGAGA ATGATCGCAG GGAGAAAAGG
    AGTCTGACTG ATAAGGAGGA GCCTCTGTCT GGGTCTGAGG TGTCCGAGGA GCAAGCTTTA
    CAGCTGGTCT GTAAAATCTT CAGAGTCTCT TGGAAGGACC GAGACAGAGA TGTTATCTTC
    CTGAATTCTC TCTCTGCTCA GTTTAAGCAG AACCCAAAAG AAGTGTTTTC GGATTTTAAG
    GACTTGATCG GACAGATCTT GATGGAGGTG CTGATGATGT CCACCCAGTC AAGGGATGAA
    AACCCATTTG CCAGTCTGAC AGCTACGTCA CAGCCAATTG CTGCAACTGC CCGGTCTCCT
    GACAGAAGCC TGATCTTAAA CACGGGCTCC AACCCAGGAA CCAGCCCTAT GTTCTGTAAT
    GTGGGCTCCT TTGGTTCCAG TTCGTTATCC AGTCTCTATG GGACTAGTCC AGCTCCTACT
    ACCAATTTCA CAAGCTATGT ACCAATGACT GGTTCATCTC TTACCCCACC AGCCAGTTCA
    GTTGCCACCA CATCTGTGCC TTCCATTACT GTCAGCTCTC ACCTCACAGC AACCAGCCCT
    TCTTGGACCC CGCCTTCCTC TCCGAGTGTG GGAGGCTCTG GCAGCTCAAG TGTTTGCGAT
    TCCTGCAGTG ACCACTTCAC CATTGAAAAC TGTAAGGAGA CAGAGATGCT GAACTACCTC
    ATTGAGTGTT TTGACCGGGT TGGAATAGAG GAGCGAAAAG CACCAAAGAT GTGTAGCCAG
    CCAACAGTGA GTCAGTTACT GAGCAACATA CGATCGCAGT GCATTTCACA TGCTGCTTTG
    GTGCTACAGG GATCCTTAAC GCAACCAAGG TCATTGCAGC AGCAGTCTTT GCTGGTACCA
    TATATGCTGT GTAGGAATCT CCCATTTGGC TTCATCCAAG AACTGGTGAG AACAACTTAC
    CAAGATGAAG AGGTGTTCAA ACAGATCTTT ATCCCCATCC TGCAAGGCCT GGCTGTAGCT
    TCCAAAGAGT GCTCCCTTGA TAGTGACAAT TTTAAGTACC CCCTTATGGC ATTATGTGAA
    CTTTGTGAAA TCAAGTTTGG AAAAACACAC CCCATGTGCA GTTTGGTTGT CTCTTTGCCC
    TTGTGGCTGC CTAAATCTTT AAGCACGGGT GCAGGAAGAG AGCTACAGAG ACTTTCCTAT
    CTTGGAGCCT TCTTCAGTCT CTCTGTCTTT GCTGAAGATG ACAACAAAGT TGTTGAAAAG
    TACTTCTCAG GACCTGCCAT CACTCTTGAA AACACCCGAG TTGTTAGCCA GTCCTTGCAG
    CATTACCTGG AACTAGCGAG GCAAGAGCTC TTCAAGATCC TGCATAGTAT TTTACTGAAT
    GGTGAAACTC GAGAGGCAGC TCTCAGTTAC ATGGCAGCTG TTGTCAACGC CAACATGAAA
    AAGGCACAAA TGCAGACAGA TGATCGTTTG GTGTCTACAG ATGGTTTTAT GCTCAACTTC
    CTATGGGTGC TGCAGCAACT AAGTATGAAG ATCAAGCTGG AAACGGTTGA TCCCATGTAC
    ATATTTCATC CCAGGTGTCG TATTGACCTC CCGACAGATG AGACAAGAGT GAAAGCAACA
    ATGGAGGATG TTACAGCCTG GATTGCTGAG CTTTACAGGG ATCCGTCTCC ATTTTCTGAT
    CCAAAATTCC CAACAGAATG TTTCTTCTTA ACCCTGCATG CCCATCATCT CTCCATCCTG
    CCGAGCTGCC GTCGGTACAT ACGTAGACTG CGAGCCATCA GGGAACTCAA CAGGACTGTT
    GAAGATCTGA AAAACAATGA AAGTCAATGG AAGGATTCTC CTCTGGCTAC CAGACACAGA
    GAGATGCTAA AACGTTGCAA GACACAACTT AAGAAACTGG TGAGGTGCAA GGCTTGTGCA
    GATGCTGGTT TGCTGGATGA GAACTTTCTC AGGAGATGTC TGAATTTCTA TGGCATGGTG
    ATTCAACTGA TGCTTCGCAT CCTCGACCCC GCCTATCCCA ACATAAAATT GCCTTTAACA
    CTTGAAGTTC CGAAGGTATT TGCAGCATTG CCAGAGTTTT ACGTGGAAGA TGTTGCTGAA
    TTTTTATTTT TTATTGTACA ATATGCTCCT CAGGTGCTTT ATGAGCCCTG TACTCAGGAC
    ATAGTGATGT TCCTTGTTGT GATGCTGTGC AACCAGAACT ACATCCGAAA TCCTTATTTA
    GTTGCTAAGC TGGTGGAGGT GATGTTCATG ACCAATCCAG CTGTTCAGCC TCGAACACAG
    AAGTTCTTTG AAATGATTGA GAATCATCCT CTGTCCACTA AATTGTTAGT CCCCTCCTTA
    ATGAAATTTT ACACAGATGT TGAGCATACT GGAGCAACTA GTGAATTCTA TGACAAGTTT
    ACAATCCGCT ACCACATCAG CACCATTTTC AAAAGCCTCT GGCAGAACAT AGCTCACCAT
    GGTACATTTA TGGAAGAGTT CAACTCTGGG AAGCAGTTTG TTCGCTACAT CAACATGCTG
    ATAAATGACA CAACTTTCTT GCTGGATGAG AGTCTGGAAT CCCTAAAACG TATCCATGAA
    GTGCAAGAGG AGATGAAGAA TAAAGAACAA TGGGACCTGC TGCCTAGGGA TCAGCAGCAG
    GCTCGGCAGT CGCAGCTGGC TCAGGATGAG CGTGTCTCTC GTTCATATCT TGCCCTGGCA
    ACAGAAACTG TTGATATGTT CCATATCCTT ACCAAGCAGG TTCAAAAGCC TTTTCTCAGA
    CCTGAACTTG GACCACGATT GGCTGCTATG TTGAACTTTA ACTTGCAGCA ACTATGTGGC
    CCGAAGTGCC GTGACCTAAA AGTTGAGAAC CCTGAGAAAT ACGGGTTTGA ACCAAAGAAG
    CTGTTGGACC AGCTGACTGA CATTTATCTA CAGCTAGACT GTGCTCGTTT TGCTAAAGCA
    ATTGCTGATG ATCAGAGGTC CTACAGCAAA GAGCTCTTTG AGGAGGTCAT ATCAAAGATG
    AGAAAGGCTG GAATCAAGTC AACCATAGCA ATAGAAAAAT TCAAACTGCT GGCAGAGAAA
    GTGGAAGAGA TTGTTGCCAA GAATGCCCGT GCAGAAATTG ATTACAGCGA TGCTCCGGAT
    GAATTCAGAG ATCCACTCAT GGACACTCTG ATGACTGATC CTGTGAGGTT GCCATCTGGA
    ACTATTATGG ACAGGTCAAT CATCCTGAGG CATCTGTTGA ATTCTTCCAC TGATCCCTTC
    AATCGTCAGA CACTGACAGA AAATATGCTG GAACCAGTGC CAGAACTTAA AGAGCAAATC
    CAGGCCTGGA TGAGAGACAA GCAGAATGCT GACCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45209
    Clone ID Related Accession (Same CDS sequence) XM_015297150.1 , XM_015297150.2
    Accession Version XM_015297150.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product ubiquitin conjugation factor E4 B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGGAGC TGAGCGCGGA CGAGATCCGT CGAAGGCGCC TTGCCCGGCT GGCAGGGGGA 
    CCATCGTCCC AGCCCAGCAC ACCGCTCACA TCGCCGCAGA GAGAGACCCC ACCTGGGCCA
    CCTGCAGCAG CTCCGACCCC GGGGCCATCC CATAGCCTCG GCCTAAATGT CCACAGCATG
    ACCCCAGCTA CCTCTCCGAT TGGTGCATCA GGAGTAGCCC ACCGAAGCCA GAGCAGCGAA
    GGGGTGAGTT CGCTCAGCAG TTCTCCATCA AACAGCCTTG AAACACAATC CCAGTCTCTC
    TCTCGGTCTC AGAGTATGGA TATTGATAGT GTCTCCTGTG AAAAAAGTAT GTCCCAGGTA
    GATGTGGATT CAGGAATTGA GAACATGGAA GTGGATGAGA ATGATCGCAG GGAGAAAAGG
    AGTCTGACTG ATAAGGAGGA GCCTCTGTCT GGGTCTGAGG TGTCCGAGGA GCAAGCTTTA
    CAGCTGGTCT GTAAAATCTT CAGAGTCTCT TGGAAGGACC GAGACAGAGA TGTTATCTTC
    CTGAATTCTC TCTCTGCTCA GTTTAAGCAG AACCCAAAAG AAGTGTTTTC GGATTTTAAG
    GACTTGATCG GACAGATCTT GATGGAGGTG CTGATGATGT CCACCCAGTC AAGGGATGAA
    AACCCATTTG CCAGTCTGAC AGCTACGTCA CAGCCAATTG CTGCAACTGC CCGGTCTCCT
    GACAGAAGCC TGATCTTAAA CACGGGCTCC AACCCAGGAA CCAGCCCTAT GTTCTGTAAT
    GTGGGCTCCT TTGGTTCCAG TTCGTTATCC AGTGTGGGAG GCTCTGGCAG CTCAAGTGTT
    TGCGATTCCT GCAGTGACCA CTTCACCATT GAAAACTGTA AGGAGACAGA GATGCTGAAC
    TACCTCATTG AGTGTTTTGA CCGGGTTGGA ATAGAGGAGC GAAAAGCACC AAAGATGTGT
    AGCCAGCCAA CAGTGAGTCA GTTACTGAGC AACATACGAT CGCAGTGCAT TTCACATGCT
    GCTTTGGTGC TACAGGGATC CTTAACGCAA CCAAGGTCAT TGCAGCAGCA GTCTTTGCTG
    GTACCATATA TGCTGTGTAG GAATCTCCCA TTTGGCTTCA TCCAAGAACT GGTGAGAACA
    ACTTACCAAG ATGAAGAGGT GTTCAAACAG ATCTTTATCC CCATCCTGCA AGGCCTGGCT
    GTAGCTTCCA AAGAGTGCTC CCTTGATAGT GACAATTTTA AGTACCCCCT TATGGCATTA
    TGTGAACTTT GTGAAATCAA GTTTGGAAAA ACACACCCCA TGTGCAGTTT GGTTGTCTCT
    TTGCCCTTGT GGCTGCCTAA ATCTTTAAGC ACGGGTGCAG GAAGAGAGCT ACAGAGACTT
    TCCTATCTTG GAGCCTTCTT CAGTCTCTCT GTCTTTGCTG AAGATGACAA CAAAGTTGTT
    GAAAAGTACT TCTCAGGACC TGCCATCACT CTTGAAAACA CCCGAGTTGT TAGCCAGTCC
    TTGCAGCATT ACCTGGAACT AGCGAGGCAA GAGCTCTTCA AGATCCTGCA TAGTATTTTA
    CTGAATGGTG AAACTCGAGA GGCAGCTCTC AGTTACATGG CAGCTGTTGT CAACGCCAAC
    ATGAAAAAGG CACAAATGCA GACAGATGAT CGTTTGGTGT CTACAGATGG TTTTATGCTC
    AACTTCCTAT GGGTGCTGCA GCAACTAAGT ATGAAGATCA AGCTGGAAAC GGTTGATCCC
    ATGTACATAT TTCATCCCAG GTGTCGTATT GACCTCCCGA CAGATGAGAC AAGAGTGAAA
    GCAACAATGG AGGATGTTAC AGCCTGGATT GCTGAGCTTT ACAGGGATCC GTCTCCATTT
    TCTGATCCAA AATTCCCAAC AGAATGTTTC TTCTTAACCC TGCATGCCCA TCATCTCTCC
    ATCCTGCCGA GCTGCCGTCG GTACATACGT AGACTGCGAG CCATCAGGGA ACTCAACAGG
    ACTGTTGAAG ATCTGAAAAA CAATGAAAGT CAATGGAAGG ATTCTCCTCT GGCTACCAGA
    CACAGAGAGA TGCTAAAACG TTGCAAGACA CAACTTAAGA AACTGGTGAG GTGCAAGGCT
    TGTGCAGATG CTGGTTTGCT GGATGAGAAC TTTCTCAGGA GATGTCTGAA TTTCTATGGC
    ATGGTGATTC AACTGATGCT TCGCATCCTC GACCCCGCCT ATCCCAACAT AAAATTGCCT
    TTAACACTTG AAGTTCCGAA GGTATTTGCA GCATTGCCAG AGTTTTACGT GGAAGATGTT
    GCTGAATTTT TATTTTTTAT TGTACAATAT GCTCCTCAGG TGCTTTATGA GCCCTGTACT
    CAGGACATAG TGATGTTCCT TGTTGTGATG CTGTGCAACC AGAACTACAT CCGAAATCCT
    TATTTAGTTG CTAAGCTGGT GGAGGTGATG TTCATGACCA ATCCAGCTGT TCAGCCTCGA
    ACACAGAAGT TCTTTGAAAT GATTGAGAAT CATCCTCTGT CCACTAAATT GTTAGTCCCC
    TCCTTAATGA AATTTTACAC AGATGTTGAG CATACTGGAG CAACTAGTGA ATTCTATGAC
    AAGTTTACAA TCCGCTACCA CATCAGCACC ATTTTCAAAA GCCTCTGGCA GAACATAGCT
    CACCATGGTA CATTTATGGA AGAGTTCAAC TCTGGGAAGC AGTTTGTTCG CTACATCAAC
    ATGCTGATAA ATGACACAAC TTTCTTGCTG GATGAGAGTC TGGAATCCCT AAAACGTATC
    CATGAAGTGC AAGAGGAGAT GAAGAATAAA GAACAATGGG ACCTGCTGCC TAGGGATCAG
    CAGCAGGCTC GGCAGTCGCA GCTGGCTCAG GATGAGCGTG TCTCTCGTTC ATATCTTGCC
    CTGGCAACAG AAACTGTTGA TATGTTCCAT ATCCTTACCA AGCAGGTTCA AAAGCCTTTT
    CTCAGACCTG AACTTGGACC ACGATTGGCT GCTATGTTGA ACTTTAACTT GCAGCAACTA
    TGTGGCCCGA AGTGCCGTGA CCTAAAAGTT GAGAACCCTG AGAAATACGG GTTTGAACCA
    AAGAAGCTGT TGGACCAGCT GACTGACATT TATCTACAGC TAGACTGTGC TCGTTTTGCT
    AAAGCAATTG CTGATGATCA GAGGTCCTAC AGCAAAGAGC TCTTTGAGGA GGTCATATCA
    AAGATGAGAA AGGCTGGAAT CAAGTCAACC ATAGCAATAG AAAAATTCAA ACTGCTGGCA
    GAGAAAGTGG AAGAGATTGT TGCCAAGAAT GCCCGTGCAG AAATTGATTA CAGCGATGCT
    CCGGATGAAT TCAGAGATCC ACTCATGGAC ACTCTGATGA CTGATCCTGT GAGGTTGCCA
    TCTGGAACTA TTATGGACAG GTCAATCATC CTGAGGCATC TGTTGAATTC TTCCACTGAT
    CCCTTCAATC GTCAGACACT GACAGAAAAT ATGCTGGAAC CAGTGCCAGA ACTTAAAGAG
    CAAATCCAGG CCTGGATGAG AGACAAGCAG AATGCTGACC ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015152636.1
    CDS102..3626
    Translation

    Target ORF information:

    RefSeq Version XM_015297150.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ubiquitination factor E4B (UBE4B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015297150.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGGAGC TGAGCGCGGA CGAGATCCGT CGAAGGCGCC TTGCCCGGCT GGCAGGGGGA 
    CCATCGTCCC AGCCCAGCAC ACCGCTCACA TCGCCGCAGA GAGAGACCCC ACCTGGGCCA
    CCTGCAGCAG CTCCGACCCC GGGGCCATCC CATAGCCTCG GCCTAAATGT CCACAGCATG
    ACCCCAGCTA CCTCTCCGAT TGGTGCATCA GGAGTAGCCC ACCGAAGCCA GAGCAGCGAA
    GGGGTGAGTT CGCTCAGCAG TTCTCCATCA AACAGCCTTG AAACACAATC CCAGTCTCTC
    TCTCGGTCTC AGAGTATGGA TATTGATAGT GTCTCCTGTG AAAAAAGTAT GTCCCAGGTA
    GATGTGGATT CAGGAATTGA GAACATGGAA GTGGATGAGA ATGATCGCAG GGAGAAAAGG
    AGTCTGACTG ATAAGGAGGA GCCTCTGTCT GGGTCTGAGG TGTCCGAGGA GCAAGCTTTA
    CAGCTGGTCT GTAAAATCTT CAGAGTCTCT TGGAAGGACC GAGACAGAGA TGTTATCTTC
    CTGAATTCTC TCTCTGCTCA GTTTAAGCAG AACCCAAAAG AAGTGTTTTC GGATTTTAAG
    GACTTGATCG GACAGATCTT GATGGAGGTG CTGATGATGT CCACCCAGTC AAGGGATGAA
    AACCCATTTG CCAGTCTGAC AGCTACGTCA CAGCCAATTG CTGCAACTGC CCGGTCTCCT
    GACAGAAGCC TGATCTTAAA CACGGGCTCC AACCCAGGAA CCAGCCCTAT GTTCTGTAAT
    GTGGGCTCCT TTGGTTCCAG TTCGTTATCC AGTGTGGGAG GCTCTGGCAG CTCAAGTGTT
    TGCGATTCCT GCAGTGACCA CTTCACCATT GAAAACTGTA AGGAGACAGA GATGCTGAAC
    TACCTCATTG AGTGTTTTGA CCGGGTTGGA ATAGAGGAGC GAAAAGCACC AAAGATGTGT
    AGCCAGCCAA CAGTGAGTCA GTTACTGAGC AACATACGAT CGCAGTGCAT TTCACATGCT
    GCTTTGGTGC TACAGGGATC CTTAACGCAA CCAAGGTCAT TGCAGCAGCA GTCTTTGCTG
    GTACCATATA TGCTGTGTAG GAATCTCCCA TTTGGCTTCA TCCAAGAACT GGTGAGAACA
    ACTTACCAAG ATGAAGAGGT GTTCAAACAG ATCTTTATCC CCATCCTGCA AGGCCTGGCT
    GTAGCTTCCA AAGAGTGCTC CCTTGATAGT GACAATTTTA AGTACCCCCT TATGGCATTA
    TGTGAACTTT GTGAAATCAA GTTTGGAAAA ACACACCCCA TGTGCAGTTT GGTTGTCTCT
    TTGCCCTTGT GGCTGCCTAA ATCTTTAAGC ACGGGTGCAG GAAGAGAGCT ACAGAGACTT
    TCCTATCTTG GAGCCTTCTT CAGTCTCTCT GTCTTTGCTG AAGATGACAA CAAAGTTGTT
    GAAAAGTACT TCTCAGGACC TGCCATCACT CTTGAAAACA CCCGAGTTGT TAGCCAGTCC
    TTGCAGCATT ACCTGGAACT AGCGAGGCAA GAGCTCTTCA AGATCCTGCA TAGTATTTTA
    CTGAATGGTG AAACTCGAGA GGCAGCTCTC AGTTACATGG CAGCTGTTGT CAACGCCAAC
    ATGAAAAAGG CACAAATGCA GACAGATGAT CGTTTGGTGT CTACAGATGG TTTTATGCTC
    AACTTCCTAT GGGTGCTGCA GCAACTAAGT ATGAAGATCA AGCTGGAAAC GGTTGATCCC
    ATGTACATAT TTCATCCCAG GTGTCGTATT GACCTCCCGA CAGATGAGAC AAGAGTGAAA
    GCAACAATGG AGGATGTTAC AGCCTGGATT GCTGAGCTTT ACAGGGATCC GTCTCCATTT
    TCTGATCCAA AATTCCCAAC AGAATGTTTC TTCTTAACCC TGCATGCCCA TCATCTCTCC
    ATCCTGCCGA GCTGCCGTCG GTACATACGT AGACTGCGAG CCATCAGGGA ACTCAACAGG
    ACTGTTGAAG ATCTGAAAAA CAATGAAAGT CAATGGAAGG ATTCTCCTCT GGCTACCAGA
    CACAGAGAGA TGCTAAAACG TTGCAAGACA CAACTTAAGA AACTGGTGAG GTGCAAGGCT
    TGTGCAGATG CTGGTTTGCT GGATGAGAAC TTTCTCAGGA GATGTCTGAA TTTCTATGGC
    ATGGTGATTC AACTGATGCT TCGCATCCTC GACCCCGCCT ATCCCAACAT AAAATTGCCT
    TTAACACTTG AAGTTCCGAA GGTATTTGCA GCATTGCCAG AGTTTTACGT GGAAGATGTT
    GCTGAATTTT TATTTTTTAT TGTACAATAT GCTCCTCAGG TGCTTTATGA GCCCTGTACT
    CAGGACATAG TGATGTTCCT TGTTGTGATG CTGTGCAACC AGAACTACAT CCGAAATCCT
    TATTTAGTTG CTAAGCTGGT GGAGGTGATG TTCATGACCA ATCCAGCTGT TCAGCCTCGA
    ACACAGAAGT TCTTTGAAAT GATTGAGAAT CATCCTCTGT CCACTAAATT GTTAGTCCCC
    TCCTTAATGA AATTTTACAC AGATGTTGAG CATACTGGAG CAACTAGTGA ATTCTATGAC
    AAGTTTACAA TCCGCTACCA CATCAGCACC ATTTTCAAAA GCCTCTGGCA GAACATAGCT
    CACCATGGTA CATTTATGGA AGAGTTCAAC TCTGGGAAGC AGTTTGTTCG CTACATCAAC
    ATGCTGATAA ATGACACAAC TTTCTTGCTG GATGAGAGTC TGGAATCCCT AAAACGTATC
    CATGAAGTGC AAGAGGAGAT GAAGAATAAA GAACAATGGG ACCTGCTGCC TAGGGATCAG
    CAGCAGGCTC GGCAGTCGCA GCTGGCTCAG GATGAGCGTG TCTCTCGTTC ATATCTTGCC
    CTGGCAACAG AAACTGTTGA TATGTTCCAT ATCCTTACCA AGCAGGTTCA AAAGCCTTTT
    CTCAGACCTG AACTTGGACC ACGATTGGCT GCTATGTTGA ACTTTAACTT GCAGCAACTA
    TGTGGCCCGA AGTGCCGTGA CCTAAAAGTT GAGAACCCTG AGAAATACGG GTTTGAACCA
    AAGAAGCTGT TGGACCAGCT GACTGACATT TATCTACAGC TAGACTGTGC TCGTTTTGCT
    AAAGCAATTG CTGATGATCA GAGGTCCTAC AGCAAAGAGC TCTTTGAGGA GGTCATATCA
    AAGATGAGAA AGGCTGGAAT CAAGTCAACC ATAGCAATAG AAAAATTCAA ACTGCTGGCA
    GAGAAAGTGG AAGAGATTGT TGCCAAGAAT GCCCGTGCAG AAATTGATTA CAGCGATGCT
    CCGGATGAAT TCAGAGATCC ACTCATGGAC ACTCTGATGA CTGATCCTGT GAGGTTGCCA
    TCTGGAACTA TTATGGACAG GTCAATCATC CTGAGGCATC TGTTGAATTC TTCCACTGAT
    CCCTTCAATC GTCAGACACT GACAGAAAAT ATGCTGGAAC CAGTGCCAGA ACTTAAAGAG
    CAAATCCAGG CCTGGATGAG AGACAAGCAG AATGCTGACC ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45209
    Clone ID Related Accession (Same CDS sequence) XM_015297150.1 , XM_015297150.2
    Accession Version XM_015297150.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ubiquitin conjugation factor E4 B isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006108.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015297150.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGGAGC TGAGCGCGGA CGAGATCCGT CGAAGGCGCC TTGCCCGGCT GGCAGGGGGA 
    CCATCGTCCC AGCCCAGCAC ACCGCTCACA TCGCCGCAGA GAGAGACCCC ACCTGGGCCA
    CCTGCAGCAG CTCCGACCCC GGGGCCATCC CATAGCCTCG GCCTAAATGT CCACAGCATG
    ACCCCAGCTA CCTCTCCGAT TGGTGCATCA GGAGTAGCCC ACCGAAGCCA GAGCAGCGAA
    GGGGTGAGTT CGCTCAGCAG TTCTCCATCA AACAGCCTTG AAACACAATC CCAGTCTCTC
    TCTCGGTCTC AGAGTATGGA TATTGATAGT GTCTCCTGTG AAAAAAGTAT GTCCCAGGTA
    GATGTGGATT CAGGAATTGA GAACATGGAA GTGGATGAGA ATGATCGCAG GGAGAAAAGG
    AGTCTGACTG ATAAGGAGGA GCCTCTGTCT GGGTCTGAGG TGTCCGAGGA GCAAGCTTTA
    CAGCTGGTCT GTAAAATCTT CAGAGTCTCT TGGAAGGACC GAGACAGAGA TGTTATCTTC
    CTGAATTCTC TCTCTGCTCA GTTTAAGCAG AACCCAAAAG AAGTGTTTTC GGATTTTAAG
    GACTTGATCG GACAGATCTT GATGGAGGTG CTGATGATGT CCACCCAGTC AAGGGATGAA
    AACCCATTTG CCAGTCTGAC AGCTACGTCA CAGCCAATTG CTGCAACTGC CCGGTCTCCT
    GACAGAAGCC TGATCTTAAA CACGGGCTCC AACCCAGGAA CCAGCCCTAT GTTCTGTAAT
    GTGGGCTCCT TTGGTTCCAG TTCGTTATCC AGTGTGGGAG GCTCTGGCAG CTCAAGTGTT
    TGCGATTCCT GCAGTGACCA CTTCACCATT GAAAACTGTA AGGAGACAGA GATGCTGAAC
    TACCTCATTG AGTGTTTTGA CCGGGTTGGA ATAGAGGAGC GAAAAGCACC AAAGATGTGT
    AGCCAGCCAA CAGTGAGTCA GTTACTGAGC AACATACGAT CGCAGTGCAT TTCACATGCT
    GCTTTGGTGC TACAGGGATC CTTAACGCAA CCAAGGTCAT TGCAGCAGCA GTCTTTGCTG
    GTACCATATA TGCTGTGTAG GAATCTCCCA TTTGGCTTCA TCCAAGAACT GGTGAGAACA
    ACTTACCAAG ATGAAGAGGT GTTCAAACAG ATCTTTATCC CCATCCTGCA AGGCCTGGCT
    GTAGCTTCCA AAGAGTGCTC CCTTGATAGT GACAATTTTA AGTACCCCCT TATGGCATTA
    TGTGAACTTT GTGAAATCAA GTTTGGAAAA ACACACCCCA TGTGCAGTTT GGTTGTCTCT
    TTGCCCTTGT GGCTGCCTAA ATCTTTAAGC ACGGGTGCAG GAAGAGAGCT ACAGAGACTT
    TCCTATCTTG GAGCCTTCTT CAGTCTCTCT GTCTTTGCTG AAGATGACAA CAAAGTTGTT
    GAAAAGTACT TCTCAGGACC TGCCATCACT CTTGAAAACA CCCGAGTTGT TAGCCAGTCC
    TTGCAGCATT ACCTGGAACT AGCGAGGCAA GAGCTCTTCA AGATCCTGCA TAGTATTTTA
    CTGAATGGTG AAACTCGAGA GGCAGCTCTC AGTTACATGG CAGCTGTTGT CAACGCCAAC
    ATGAAAAAGG CACAAATGCA GACAGATGAT CGTTTGGTGT CTACAGATGG TTTTATGCTC
    AACTTCCTAT GGGTGCTGCA GCAACTAAGT ATGAAGATCA AGCTGGAAAC GGTTGATCCC
    ATGTACATAT TTCATCCCAG GTGTCGTATT GACCTCCCGA CAGATGAGAC AAGAGTGAAA
    GCAACAATGG AGGATGTTAC AGCCTGGATT GCTGAGCTTT ACAGGGATCC GTCTCCATTT
    TCTGATCCAA AATTCCCAAC AGAATGTTTC TTCTTAACCC TGCATGCCCA TCATCTCTCC
    ATCCTGCCGA GCTGCCGTCG GTACATACGT AGACTGCGAG CCATCAGGGA ACTCAACAGG
    ACTGTTGAAG ATCTGAAAAA CAATGAAAGT CAATGGAAGG ATTCTCCTCT GGCTACCAGA
    CACAGAGAGA TGCTAAAACG TTGCAAGACA CAACTTAAGA AACTGGTGAG GTGCAAGGCT
    TGTGCAGATG CTGGTTTGCT GGATGAGAAC TTTCTCAGGA GATGTCTGAA TTTCTATGGC
    ATGGTGATTC AACTGATGCT TCGCATCCTC GACCCCGCCT ATCCCAACAT AAAATTGCCT
    TTAACACTTG AAGTTCCGAA GGTATTTGCA GCATTGCCAG AGTTTTACGT GGAAGATGTT
    GCTGAATTTT TATTTTTTAT TGTACAATAT GCTCCTCAGG TGCTTTATGA GCCCTGTACT
    CAGGACATAG TGATGTTCCT TGTTGTGATG CTGTGCAACC AGAACTACAT CCGAAATCCT
    TATTTAGTTG CTAAGCTGGT GGAGGTGATG TTCATGACCA ATCCAGCTGT TCAGCCTCGA
    ACACAGAAGT TCTTTGAAAT GATTGAGAAT CATCCTCTGT CCACTAAATT GTTAGTCCCC
    TCCTTAATGA AATTTTACAC AGATGTTGAG CATACTGGAG CAACTAGTGA ATTCTATGAC
    AAGTTTACAA TCCGCTACCA CATCAGCACC ATTTTCAAAA GCCTCTGGCA GAACATAGCT
    CACCATGGTA CATTTATGGA AGAGTTCAAC TCTGGGAAGC AGTTTGTTCG CTACATCAAC
    ATGCTGATAA ATGACACAAC TTTCTTGCTG GATGAGAGTC TGGAATCCCT AAAACGTATC
    CATGAAGTGC AAGAGGAGAT GAAGAATAAA GAACAATGGG ACCTGCTGCC TAGGGATCAG
    CAGCAGGCTC GGCAGTCGCA GCTGGCTCAG GATGAGCGTG TCTCTCGTTC ATATCTTGCC
    CTGGCAACAG AAACTGTTGA TATGTTCCAT ATCCTTACCA AGCAGGTTCA AAAGCCTTTT
    CTCAGACCTG AACTTGGACC ACGATTGGCT GCTATGTTGA ACTTTAACTT GCAGCAACTA
    TGTGGCCCGA AGTGCCGTGA CCTAAAAGTT GAGAACCCTG AGAAATACGG GTTTGAACCA
    AAGAAGCTGT TGGACCAGCT GACTGACATT TATCTACAGC TAGACTGTGC TCGTTTTGCT
    AAAGCAATTG CTGATGATCA GAGGTCCTAC AGCAAAGAGC TCTTTGAGGA GGTCATATCA
    AAGATGAGAA AGGCTGGAAT CAAGTCAACC ATAGCAATAG AAAAATTCAA ACTGCTGGCA
    GAGAAAGTGG AAGAGATTGT TGCCAAGAAT GCCCGTGCAG AAATTGATTA CAGCGATGCT
    CCGGATGAAT TCAGAGATCC ACTCATGGAC ACTCTGATGA CTGATCCTGT GAGGTTGCCA
    TCTGGAACTA TTATGGACAG GTCAATCATC CTGAGGCATC TGTTGAATTC TTCCACTGAT
    CCCTTCAATC GTCAGACACT GACAGAAAAT ATGCTGGAAC CAGTGCCAGA ACTTAAAGAG
    CAAATCCAGG CCTGGATGAG AGACAAGCAG AATGCTGACC ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015152636.1
    CDS117..3641
    Translation

    Target ORF information:

    RefSeq Version XM_015297150.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ubiquitination factor E4B (UBE4B), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015297150.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGGAGC TGAGCGCGGA CGAGATCCGT CGAAGGCGCC TTGCCCGGCT GGCAGGGGGA 
    CCATCGTCCC AGCCCAGCAC ACCGCTCACA TCGCCGCAGA GAGAGACCCC ACCTGGGCCA
    CCTGCAGCAG CTCCGACCCC GGGGCCATCC CATAGCCTCG GCCTAAATGT CCACAGCATG
    ACCCCAGCTA CCTCTCCGAT TGGTGCATCA GGAGTAGCCC ACCGAAGCCA GAGCAGCGAA
    GGGGTGAGTT CGCTCAGCAG TTCTCCATCA AACAGCCTTG AAACACAATC CCAGTCTCTC
    TCTCGGTCTC AGAGTATGGA TATTGATAGT GTCTCCTGTG AAAAAAGTAT GTCCCAGGTA
    GATGTGGATT CAGGAATTGA GAACATGGAA GTGGATGAGA ATGATCGCAG GGAGAAAAGG
    AGTCTGACTG ATAAGGAGGA GCCTCTGTCT GGGTCTGAGG TGTCCGAGGA GCAAGCTTTA
    CAGCTGGTCT GTAAAATCTT CAGAGTCTCT TGGAAGGACC GAGACAGAGA TGTTATCTTC
    CTGAATTCTC TCTCTGCTCA GTTTAAGCAG AACCCAAAAG AAGTGTTTTC GGATTTTAAG
    GACTTGATCG GACAGATCTT GATGGAGGTG CTGATGATGT CCACCCAGTC AAGGGATGAA
    AACCCATTTG CCAGTCTGAC AGCTACGTCA CAGCCAATTG CTGCAACTGC CCGGTCTCCT
    GACAGAAGCC TGATCTTAAA CACGGGCTCC AACCCAGGAA CCAGCCCTAT GTTCTGTAAT
    GTGGGCTCCT TTGGTTCCAG TTCGTTATCC AGTGTGGGAG GCTCTGGCAG CTCAAGTGTT
    TGCGATTCCT GCAGTGACCA CTTCACCATT GAAAACTGTA AGGAGACAGA GATGCTGAAC
    TACCTCATTG AGTGTTTTGA CCGGGTTGGA ATAGAGGAGC GAAAAGCACC AAAGATGTGT
    AGCCAGCCAA CAGTGAGTCA GTTACTGAGC AACATACGAT CGCAGTGCAT TTCACATGCT
    GCTTTGGTGC TACAGGGATC CTTAACGCAA CCAAGGTCAT TGCAGCAGCA GTCTTTGCTG
    GTACCATATA TGCTGTGTAG GAATCTCCCA TTTGGCTTCA TCCAAGAACT GGTGAGAACA
    ACTTACCAAG ATGAAGAGGT GTTCAAACAG ATCTTTATCC CCATCCTGCA AGGCCTGGCT
    GTAGCTTCCA AAGAGTGCTC CCTTGATAGT GACAATTTTA AGTACCCCCT TATGGCATTA
    TGTGAACTTT GTGAAATCAA GTTTGGAAAA ACACACCCCA TGTGCAGTTT GGTTGTCTCT
    TTGCCCTTGT GGCTGCCTAA ATCTTTAAGC ACGGGTGCAG GAAGAGAGCT ACAGAGACTT
    TCCTATCTTG GAGCCTTCTT CAGTCTCTCT GTCTTTGCTG AAGATGACAA CAAAGTTGTT
    GAAAAGTACT TCTCAGGACC TGCCATCACT CTTGAAAACA CCCGAGTTGT TAGCCAGTCC
    TTGCAGCATT ACCTGGAACT AGCGAGGCAA GAGCTCTTCA AGATCCTGCA TAGTATTTTA
    CTGAATGGTG AAACTCGAGA GGCAGCTCTC AGTTACATGG CAGCTGTTGT CAACGCCAAC
    ATGAAAAAGG CACAAATGCA GACAGATGAT CGTTTGGTGT CTACAGATGG TTTTATGCTC
    AACTTCCTAT GGGTGCTGCA GCAACTAAGT ATGAAGATCA AGCTGGAAAC GGTTGATCCC
    ATGTACATAT TTCATCCCAG GTGTCGTATT GACCTCCCGA CAGATGAGAC AAGAGTGAAA
    GCAACAATGG AGGATGTTAC AGCCTGGATT GCTGAGCTTT ACAGGGATCC GTCTCCATTT
    TCTGATCCAA AATTCCCAAC AGAATGTTTC TTCTTAACCC TGCATGCCCA TCATCTCTCC
    ATCCTGCCGA GCTGCCGTCG GTACATACGT AGACTGCGAG CCATCAGGGA ACTCAACAGG
    ACTGTTGAAG ATCTGAAAAA CAATGAAAGT CAATGGAAGG ATTCTCCTCT GGCTACCAGA
    CACAGAGAGA TGCTAAAACG TTGCAAGACA CAACTTAAGA AACTGGTGAG GTGCAAGGCT
    TGTGCAGATG CTGGTTTGCT GGATGAGAAC TTTCTCAGGA GATGTCTGAA TTTCTATGGC
    ATGGTGATTC AACTGATGCT TCGCATCCTC GACCCCGCCT ATCCCAACAT AAAATTGCCT
    TTAACACTTG AAGTTCCGAA GGTATTTGCA GCATTGCCAG AGTTTTACGT GGAAGATGTT
    GCTGAATTTT TATTTTTTAT TGTACAATAT GCTCCTCAGG TGCTTTATGA GCCCTGTACT
    CAGGACATAG TGATGTTCCT TGTTGTGATG CTGTGCAACC AGAACTACAT CCGAAATCCT
    TATTTAGTTG CTAAGCTGGT GGAGGTGATG TTCATGACCA ATCCAGCTGT TCAGCCTCGA
    ACACAGAAGT TCTTTGAAAT GATTGAGAAT CATCCTCTGT CCACTAAATT GTTAGTCCCC
    TCCTTAATGA AATTTTACAC AGATGTTGAG CATACTGGAG CAACTAGTGA ATTCTATGAC
    AAGTTTACAA TCCGCTACCA CATCAGCACC ATTTTCAAAA GCCTCTGGCA GAACATAGCT
    CACCATGGTA CATTTATGGA AGAGTTCAAC TCTGGGAAGC AGTTTGTTCG CTACATCAAC
    ATGCTGATAA ATGACACAAC TTTCTTGCTG GATGAGAGTC TGGAATCCCT AAAACGTATC
    CATGAAGTGC AAGAGGAGAT GAAGAATAAA GAACAATGGG ACCTGCTGCC TAGGGATCAG
    CAGCAGGCTC GGCAGTCGCA GCTGGCTCAG GATGAGCGTG TCTCTCGTTC ATATCTTGCC
    CTGGCAACAG AAACTGTTGA TATGTTCCAT ATCCTTACCA AGCAGGTTCA AAAGCCTTTT
    CTCAGACCTG AACTTGGACC ACGATTGGCT GCTATGTTGA ACTTTAACTT GCAGCAACTA
    TGTGGCCCGA AGTGCCGTGA CCTAAAAGTT GAGAACCCTG AGAAATACGG GTTTGAACCA
    AAGAAGCTGT TGGACCAGCT GACTGACATT TATCTACAGC TAGACTGTGC TCGTTTTGCT
    AAAGCAATTG CTGATGATCA GAGGTCCTAC AGCAAAGAGC TCTTTGAGGA GGTCATATCA
    AAGATGAGAA AGGCTGGAAT CAAGTCAACC ATAGCAATAG AAAAATTCAA ACTGCTGGCA
    GAGAAAGTGG AAGAGATTGT TGCCAAGAAT GCCCGTGCAG AAATTGATTA CAGCGATGCT
    CCGGATGAAT TCAGAGATCC ACTCATGGAC ACTCTGATGA CTGATCCTGT GAGGTTGCCA
    TCTGGAACTA TTATGGACAG GTCAATCATC CTGAGGCATC TGTTGAATTC TTCCACTGAT
    CCCTTCAATC GTCAGACACT GACAGAAAAT ATGCTGGAAC CAGTGCCAGA ACTTAAAGAG
    CAAATCCAGG CCTGGATGAG AGACAAGCAG AATGCTGACC ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.