TSKU cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	TSKU
Entrez Gene ID	419088
Full Name	tsukushi, small leucine rich proteoglycan
Synonyms	C-TSK,C-TSKB,EIIH,LRRC54
General protein information	Preferred Names tsukushi, small leucine rich proteoglycan Names tsukushin leucine rich repeat containing 54 leucine-rich repeat-containing protein 54 tsukushi B tsukushi small leucine rich proteoglycan homolog
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001005346.1	NP_001005346.1	tsukushin precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Tsku	NP_001009965.1
Gallus gallus (chicken)	TSKU	NP_001005346.1
Mus musculus (house mouse)	Tsku	NP_001162011.1
Canis lupus familiaris (dog)	TSKU	XP_005633522.1
Homo sapiens (human)	TSKU	NP_001245139.1
Xenopus tropicalis (tropical clawed frog)	tsku	NP_001011330.1
Pan troglodytes (chimpanzee)	TSKU	XP_508654.3
	TSKU	XP_002799755.1
Danio rerio (zebrafish)	tsku	NP_956027.1

Gene Ontology
Bibliography

Related articles in PubMed

Tsukushi functions as a Wnt signaling inhibitor by competing with Wnt2b for binding to transmembrane protein Frizzled4.
Ohta K, Ito A, Kuriyama S, Lupo G, Kosaka M, Ohnuma S, Nakagawa S, Tanaka H
Proceedings of the National Academy of Sciences of the United States of America108(36)14962-7(2011 Sep)

Anterior shift in gene expression precedes anteriormost digit formation in amniote limbs.
Uejima A, Amano T, Nomura N, Noro M, Yasue T, Shiroishi T, Ohta K, Yokoyama H, Tamura K
Development, growth & differentiation52(2)223-34(2010 Feb)

Tsukushi cooperates with VG1 to induce primitive streak and Hensen's node formation in the chick embryo.
Ohta K, Kuriyama S, Okafuji T, Gejima R, Ohnuma S, Tanaka H
Development (Cambridge, England)133(19)3777-86(2006 Oct)

Tsukushi controls ectodermal patterning and neural crest specification in Xenopus by direct regulation of BMP4 and X-delta-1 activity.
Kuriyama S, Lupo G, Ohta K, Ohnuma S, Harris WA, Tanaka H
Development (Cambridge, England)133(1)75-88(2006 Jan)

Tsukushi functions as an organizer inducer by inhibition of BMP activity in cooperation with chordin.
Ohta K, Lupo G, Kuriyama S, Keynes R, Holt CE, Harris WA, Tanaka H, Ohnuma SI
Developmental cell7(3)347-358(2004 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

targeted inactivation of the TSK gene in mice causes expansion of the ciliary body and up-regulation of Wnt2b and Fzd4 expression in the developing peripheral eye
Title: Tsukushi functions as a Wnt signaling inhibitor by competing with Wnt2b for binding to transmembrane protein Frizzled4.

The anterior expression is not fixed in the period of limb development, but the expression domains of Mkp3, Sef and TSK change considerably from the distal domain to the anterior domain
Title: Anterior shift in gene expression precedes anteriormost digit formation in amniote limbs.

TSK isoforms play a crucial role in chick axis formation by locally modulating VG1 and BMP activities during gastrulation.
Title: Tsukushi cooperates with VG1 to induce primitive streak and Hensen's node formation in the chick embryo.

The following TSKU gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSKU cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa27060	NM_001005346.1 Latest version!	Gallus gallus tsukushi, small leucine rich proteoglycan (TSKU), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TSKU ( NM_001005346.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001005346.1 Gallus gallus tsukushi, small leucine rich proteoglycan (TSKU), mRNA.

Price & Availability↑

CloneID	OGa27060
Clone ID Related Accession (Same CDS sequence)	NM_001005346.1
Accession Version	NM_001005346.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Gallus gallus(chicken)
Product	tsukushin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB100033.1. On Sep 27, 2004 this sequence version replaced XM_417280.1. ##Evidence-Data-START## Transcript exon combination :: AB100033.1 [ECO:0000332] ##Evidence-Data-END##

ATGCAGTTCC TAGCCTGGTT CAACATGCTG CTTCTCCTGC CGTGTTTCAG TACCACCAAA 
ACCTGCTTCC CTGGCTGCCA CTGTGAAGTG GAAAGCTTTG GTCTCTTCGA CAGCTTTAGC 
CTGACCAAGG TGGACTGCAG CGGGATAGGC TCACACATTG TTCCTGTCCC AATCCCACTG 
GATACCTCCT ACTTGGATTT GTCCTCAAAC AAACTGGAAA CAATCAATGA ATCAATGCTC 
ACTGGCCCTG GATACACCAC CCTGGTGAGC CTCGACCTGA GCTACAATAA CATCGCCAAG 
ATTTCCTCCA CAACCTTCTC CAGGCTTCGC TACCTGGAGT CTTTGGATCT CAGTCATAAC 
TCTCTGGAAG TCCTTCCAGA GGACTGCTTC TCCAGTTCTC CTTTGGGCGA CATAGATTTG 
AGCAATAACA AGCTTTTGGA TATAGCGCTG GATGTTTTTG CTTCAAAGGG TCAAGGAAAA 
CCCTTGAATG TGGATCTATC CAACAACATG CTCAGCAAAA TTACACGGAA CCATGAAAAG 
AGCATCCCCA ACATCCAGAA CTTAAATCTT TCTGGAAACA GGCTAACATC TGTGCCAAAC 
CTCCAAGGCA TTCCTCTTCG GTACTTAAAT CTTGATGGCA ACCCGCTGGC CAAGATTGAG 
AAAGGAGACT TCAAGGGGCT GAAAGGTTTG ATTCATTTAT CCCTCAGCGG CCTCCATGAC 
TTCAGAGAGT TATCTCCTTA CAGTTTCAAG GAATTACCAG CCCTCCAAGT TCTGGATTTA 
TCCAACAATC CCAACCTAAG GTCATTGACT GCTGAAGTTA TCTTTGGCCT GAACTCCATA 
CAAGAGCTCA ACCTCTCTGG GACTGGCGTG TCGTCCTTGC CAAAGACTGT GCTGAAATAC 
CTACCTTCCC TCAAAAGCAT CACCTTGAGG AAGAACATTC AGTGTTTTAA GACCATCAAA 
GAAGGACAGT ACCACCGACA AATTGGGCTG ACCAAGCTGG GTCTTGGGGC AGAAGAGCTG 
CTGTGGTGCA AAACTCCATG CCCCAGACCA GTCTGCAGGT GCAGAGATAA GCCTCTCCAA 
AGTGCACCCC AAAACCTGCC AACTCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005346.1
CDS	142..1251
Translation	MQFLAWFNMLLLLPCFSTTKTCFPGCHCEVESFGLFDSFSLTKVDCSGIGSHIVPVPIPLDTSYLDLSSNKLETINESMLTGPGYTTLVSLDLSYNNIAKISSTTFSRLRYLESLDLSHNSLEVLPEDCFSSSPLGDIDLSNNKLLDIALDVFASKGQGKPLNVDLSNNMLSKITRNHEKSIPNIQNLNLSGNRLTSVPNLQGIPLRYLNLDGNPLAKIEKGDFKGLKGLIHLSLSGLHDFRELSPYSFKELPALQVLDLSNNPNLRSLTAEVIFGLNSIQELNLSGTGVSSLPKTVLKYLPSLKSITLRKNIQCFKTIKEGQYHRQIGLTKLGLGAEELLWCKTPCPRPVCRCRDKPLQSAPQNLPTP

Target ORF information:

RefSeq Version	NM_001005346.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tsukushi, small leucine rich proteoglycan (TSKU), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005346.1

ORF Insert Sequence:

ATGCAGTTCC TAGCCTGGTT CAACATGCTG CTTCTCCTGC CGTGTTTCAG TACCACCAAA 
ACCTGCTTCC CTGGCTGCCA CTGTGAAGTG GAAAGCTTTG GTCTCTTCGA CAGCTTTAGC 
CTGACCAAGG TGGACTGCAG CGGGATAGGC TCACACATTG TTCCTGTCCC AATCCCACTG 
GATACCTCCT ACTTGGATTT GTCCTCAAAC AAACTGGAAA CAATCAATGA ATCAATGCTC 
ACTGGCCCTG GATACACCAC CCTGGTGAGC CTCGACCTGA GCTACAATAA CATCGCCAAG 
ATTTCCTCCA CAACCTTCTC CAGGCTTCGC TACCTGGAGT CTTTGGATCT CAGTCATAAC 
TCTCTGGAAG TCCTTCCAGA GGACTGCTTC TCCAGTTCTC CTTTGGGCGA CATAGATTTG 
AGCAATAACA AGCTTTTGGA TATAGCGCTG GATGTTTTTG CTTCAAAGGG TCAAGGAAAA 
CCCTTGAATG TGGATCTATC CAACAACATG CTCAGCAAAA TTACACGGAA CCATGAAAAG 
AGCATCCCCA ACATCCAGAA CTTAAATCTT TCTGGAAACA GGCTAACATC TGTGCCAAAC 
CTCCAAGGCA TTCCTCTTCG GTACTTAAAT CTTGATGGCA ACCCGCTGGC CAAGATTGAG 
AAAGGAGACT TCAAGGGGCT GAAAGGTTTG ATTCATTTAT CCCTCAGCGG CCTCCATGAC 
TTCAGAGAGT TATCTCCTTA CAGTTTCAAG GAATTACCAG CCCTCCAAGT TCTGGATTTA 
TCCAACAATC CCAACCTAAG GTCATTGACT GCTGAAGTTA TCTTTGGCCT GAACTCCATA 
CAAGAGCTCA ACCTCTCTGG GACTGGCGTG TCGTCCTTGC CAAAGACTGT GCTGAAATAC 
CTACCTTCCC TCAAAAGCAT CACCTTGAGG AAGAACATTC AGTGTTTTAA GACCATCAAA 
GAAGGACAGT ACCACCGACA AATTGGGCTG ACCAAGCTGG GTCTTGGGGC AGAAGAGCTG 
CTGTGGTGCA AAACTCCATG CCCCAGACCA GTCTGCAGGT GCAGAGATAA GCCTCTCCAA 
AGTGCACCCC AAAACCTGCC AACTCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tsukushi functions as a Wnt signaling inhibitor by competing with Wnt2b for binding to transmembrane protein Frizzled4.
Proceedings of the National Academy of Sciences of the United States of America108(36)14962-7(2011 Sep)
Ohta K,Ito A,Kuriyama S,Lupo G,Kosaka M,Ohnuma S,Nakagawa S,Tanaka H

Anterior shift in gene expression precedes anteriormost digit formation in amniote limbs.
Development, growth & differentiation52(2)223-34(2010 Feb)
Uejima A,Amano T,Nomura N,Noro M,Yasue T,Shiroishi T,Ohta K,Yokoyama H,Tamura K

Tsukushi cooperates with VG1 to induce primitive streak and Hensen's node formation in the chick embryo.
Development (Cambridge, England)133(19)3777-86(2006 Oct)
Ohta K,Kuriyama S,Okafuji T,Gejima R,Ohnuma S,Tanaka H

Tsukushi controls ectodermal patterning and neural crest specification in Xenopus by direct regulation of BMP4 and X-delta-1 activity.
Development (Cambridge, England)133(1)75-88(2006 Jan)
Kuriyama S,Lupo G,Ohta K,Ohnuma S,Harris WA,Tanaka H

Tsukushi functions as an organizer inducer by inhibition of BMP activity in cooperation with chordin.
Developmental cell7(3)347-358(2004 Sep)
Ohta K,Lupo G,Kuriyama S,Keynes R,Holt CE,Harris WA,Tanaka H,Ohnuma SI

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TSKU cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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