TNRC6B gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_015288794.2	XP_015144280.1	trinucleotide repeat-containing gene 6B protein isoform X4
XM_015288805.2	XP_015144291.1	trinucleotide repeat-containing gene 6B protein isoform X7
XM_025155217.1	XP_025010985.1	trinucleotide repeat-containing gene 6B protein isoform X2
XM_004937725.2	XP_004937782.1	trinucleotide repeat-containing gene 6B protein isoform X4
XM_015288813.1	XP_015144299.1	trinucleotide repeat-containing gene 6B protein isoform X8
XM_004937731.2	XP_004937788.2	trinucleotide repeat-containing gene 6B protein isoform X9
XM_004937722.3	XP_004937779.1	trinucleotide repeat-containing gene 6B protein isoform X1
XM_015288799.2	XP_015144285.1	trinucleotide repeat-containing gene 6B protein isoform X5
XM_025155203.1	XP_025010971.1	trinucleotide repeat-containing gene 6B protein isoform X6
XM_004937723.2	XP_004937780.1	trinucleotide repeat-containing gene 6B protein isoform X2
XM_015288799.1	XP_015144285.1	trinucleotide repeat-containing gene 6B protein isoform X6
XM_015288805.1	XP_015144291.1	trinucleotide repeat-containing gene 6B protein isoform X7
XM_004937725.3	XP_004937782.1	trinucleotide repeat-containing gene 6B protein isoform X3
XM_015288813.2	XP_015144299.1	trinucleotide repeat-containing gene 6B protein isoform X8
XM_004937731.3	XP_004937788.2	trinucleotide repeat-containing gene 6B protein isoform X9
XM_004937722.2	XP_004937779.1	trinucleotide repeat-containing gene 6B protein isoform X1
XM_004937724.2	XP_004937781.2	trinucleotide repeat-containing gene 6B protein isoform X3
XM_015288794.1	XP_015144280.1	trinucleotide repeat-containing gene 6B protein isoform X5
XM_004937723.3	XP_004937780.1	trinucleotide repeat-containing gene 6B protein isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa01767	XM_004937722.3 Latest version!	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa01768	XM_004937723.3 Latest version!	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa01768	XM_025155217.1 Latest version!	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa01770	XM_004937725.3 Latest version!	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa01774	XM_025155203.1 Latest version!	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa29592	XM_004937724.2 Latest version!	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa29593	XM_015288794.2 Latest version!	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa29594	XM_015288799.2 Latest version!	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa29595	XM_015288805.2 Latest version!	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa29596	XM_015288813.2 Latest version!	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa29597	XM_004937731.3 Latest version!	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
View Old Accession Versions >>
OGa29594	XM_015288799.1	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa29595	XM_015288805.1	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa01770	XM_004937725.2	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa29596	XM_015288813.1	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa29597	XM_004937731.2	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa01767	XM_004937722.2	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa29593	XM_015288794.1	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa01768	XM_004937723.2	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

TNRC6B ( XM_004937722.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004937779.1 Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa01767
Clone ID Related Accession (Same CDS sequence)	XM_004937722.3 , XM_004937722.2
Accession Version	XM_004937722.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	trinucleotide repeat-containing gene 6B protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004937722.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACTG ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGATGGA ACATGAGGAT 
TTTGTAATGG AGGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAACAA ACAAGAGAAG 
GAGCAAGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAGGA GGATAAGAAA 
AAGAAGGAAT CTGCTCAGAA GGTCACAGAT CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGT GGCTGCCAGC CCAATTGGCA ACTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTGAAACGTG GGCAGCCCCC TCCACCATCC TGTATGCTCC TTGGGGGTGG AGCAGGGCCT 
CCTCCCCCTA CAGCACCAGG AGCAAACCCA AACAACGCAC AACCAGTGAC AGGAGCACTG 
CTGCAGAGCG ACAGTGGGAC TGCAACAGAT TCAACAATTG GAGGTGCTGC TGCTTCAAAT 
TATGCAAATT CCACTTGGGG TCCTGGAGCC TCCTCCAGCA GCGGCACCAA CGCCAACCCA 
ACTCACATCT GGGACAAGGT GATTGTAGAC GGGTCTGACA TGGAAGAGTG GCCTTGTATC 
GCCAGCAAAG ATGCTGAGTC TTCTTCCGAA AACACCACCG ATAACAACAG TGCCTCGAAC 
CCTGGCTTGG AGAAGAACAC TCTGCCAGGA AGCACCACTA GTAACAAAGG AAAAGGAAGC 
CAGTGCCAGT CTGGAAGCGC TGGAAATGAA TGTAATCTTG GGGCCTGGAA ATCTGATCCC 
AAGGCTAAAT CTGTTCAATC TTCCAACCCT GCTACCGAGA GTAACAGTGG ACTAAGTAAT 
TGGAGGAACT TGACTGGACA AGATAGAATT GGTTCTGGTT CTGGCTTCAG CAACTTTAAC 
CCAAATAGCA ACCCGTCTGC TTGGCCAGCA CTGGTCCAAG AGGGCAATTC TAGGAAAGGG 
ACTTCGGAAT CTGATAGTGG TAGCTCCAAT GCACAGATTA GCACAGTAGG TCAGACCTCT 
AGGGAACAGC AGTCAAAGAT GGAAAATGCG GGTGTTAACT TTGTCTCTGG CAGAGAACAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTTAAGT 
TCACCAAACC CTATGGAGAA TAAGGGAATG CCCTTTGAAA TGGGCTTGGG GAACCCTTCC 
AGGAGCACTG ACACCCCTTC ACAAAGCACT GGAGAAAGAA AGACTGGGAG TGTTGGATCT 
TGGGGTACAG CTAGGGGGTC TTCTGGAACT GACACAGTCT CTGGACAGAG CAATTCTGGA 
AACCATGGGA ACAATGGAAA GGATAGGGAG GACTCCTGGA AAGGAGCTTC TGTTCAAAAA 
CCTAATGGGT CAAGAAGCGA TTCTTGGGAT AACAATAACC GGTCTACGGG TGGTGGGTCT 
TGGAACTTTG GCCCTCAGCA TGCAAATGAA AGCAAATGGG GTGAAGGGAA CAAATTAACA 
TCTGGGGTCT CTCAGGGAGA ATGGAAACAG CTGTCTGGGT CTGATGAACT GAAGATTGGA 
GAATGGAGTG GTCCAAACCA ACCAAATTCT AGCACTGGAG CATGGGACAA TCAAAAAGGC 
CACCCTCTTC CTGAAAACCA AGGCAATTCC CAGGCTCCCT GTTGGGGAAG ATCTTCCAGC 
TCTGCAGGAA GTGAGGTTGG AGGTCAAAGC ACTGGAAGCA ACCACAAAGC AGGAAGTAGT 
GACAGTCACA ATTCTGGACG CCGATCATAC AGGCCTACGC ATCCTGATTG CCAGGCAGTC 
TTGCAGACTT TGCTGAGCAG GACTGATTTG GACCCCCGAG TGCTTTCAAA CACTGGCTGG 
GGCCAAACTC AAATTAAGCA AGATACCGTA TGGGATATTG AAGAGATGCC ACGGTCCGAG 
GGGAAGTCTG ATAAAGGAAC TGAGGGGTGG GAGAGCTCTG CCACACAGAC AAAGAACTCA 
GGGGGCTGGG GCGATGTACC CAGCCAAAGC AATCAAATCA AGTCTGGATG GGGTGAGCTC 
TCAACCCCAA CAGAATGGAA GGACCCCAAA AATACTGGAG GATGGAATGA CTATAAGAAC 
CCCAATTCGT CTAATTGGGG AGGGGGAAGA CAAGAAGAGA AATCGTCATC TTCTTGGAAT 
GACAGCTCTA GTAAGGATCA GGGGTGGGGT GGACGGCAGC CTAATCAAGG ATGGTCTTCT 
GGAAAGAATG GTTGGGGAGA GGAAGTTGAC CAAGCAAAAA ACAGCAACTG GGAAAGTGCG 
GGAAATAAGC CAGCATCTGG TTGGGGTGAA GGTGGGCAAA ATGAGATTGG AACTTGGGGT 
AACGGTGCAA ATACAGGCTC TGCTTCAAAG GGTGGTTGGG ATGATTGTAA AAGAAATTCA 
ACGTGGAACG AGACGGGTCG ACAGCCCAAC TCCTGGAACA AGCAGCAGCA GCAGCAACAG 
CAGCAACAGC AAGAGACTTC TGGTTCCTGG GGTCCACCGC CACAAACTCC AAGTAATGGG 
CGACCTCCAA ACCCAAACTG GAACAGCGGA CCACAACCAG CTGCCCCCAA AGACGAGGAG 
CCTAGTGGCT GGGAAGAACC TTCTCCACAG TCAATCAGTC GAAAAATGGA TATTGATGAT 
GGCACTTCAG CGTGGGGAGA TCCTAGCAGT TATAACTACA AGAATGTGAA CCTGTGGGAC 
AAGAACTCAC AAGGGGGACA GGCCCCACGG GAACAGAGCC TGCCTACTCC AATGACTAGC 
AAATCACAAG CATCAGTCTG GAGCAAAAGC ACTCCACCTG CTCCAGATAA TGGTACGTCC 
GCCTGGGGTG AGCCAAATGA AACCAGTCCC GGGTGGGGTG AAGTAGATGA TACAGGAGCA 
TCGACAACAG GCTGGGGGAA TGCACCTTCC AACGCCCCGA ATGCCATGAA ATCTAGTTCT 
AAATCTATGC AAGATGGCTG GGGAGAGAGT GATGGGCCAG TGACAGGAAC ACGTCATTCC 
AGCTGGGAAG AGGAGGAGGA GGGAGGAGTC TGGAACACAG CAGGCTCTCA GGGAAGCAGT 
TCCTCCCACA ACTCAACAAG CTGGGGGCAA GGAGGAAAGA AAACGCAGAT GAAGTGCTCG 
TTAAAAGGAG GAAATAGTGA TTCATGGATG AACCCTATAT CCAAGCAGTT TTCAAATATG 
GGCTTGCTAA GTCAAACTGA GGACATTCCA GGCAATAAGA TGGACTTGTC TGTAGGTGGT 
CTCCCAGATA AGAAGTTTGA GGTAGATAAA CGAGCCATGA ATCTCGGGGA TTTTAATGAC 
ATAATGAGAA AGGATCGATC TGGGTTCCGT CCACCTAATT CCAAAGACAT GGGAACCACA 
GATAGTGGGC CTTATTTTGA GAAGCTGACT TTGCCTTTCT CCAATCAAGA TGGGTGCCTT 
GGGGATGAGG CTCCCTGCTC TCCCTTCTCC CCTTCTCCCA GCTACAAGCT GTCTCCCTCT 
GGTTCCACAC TATCCAACGT CGGCCTTGGA GCAATCGGCT CAGGGCTCAG TCCCCAAAAC 
TACGCTGCTA GACAAGGTGG CAATCATGGT TTGTTTGGAA ACAGCACAGC ACAATCGAGG 
GGCATGCACA CACCAGTGCA GCCACTAAAT CCTTCCCCCA ATATCCGGGC GCAAGTGCCT 
CCCCAATTTC TTTCTCCCCA GGTTTCGGCC TCCATGCTCA AACAGTTCCC CAACAGTGGC 
TTGAATCCAG GTCTTTTCAA TGTGGGGCCC CAGCTTTCTC CACAACAGAT TGCCATGCTG 
AGCCAGCTTC CACAGATTCC CCAGTTTCAA TTAGCATGTC AGCTCCTCCT TCAGCAGCAG 
CAGCAGCAGC AGCAGCTGTT ACAGAGCCAG AGGAAGTTAT CTCAAGCTGT GCGACAGCAG 
CAGGAGCAAC AGATTGCTCG TATGGTGAGT GCCCTCCAGC AGCAGCAGCA GAGGCAGCCT 
GGCATGAAGC ATTCACCATC CCACCCTGTT GGGCCTAAGC CACATATAGA CAGCATGGTA 
CCCAATCCTT TGAATGTGGG TCTTTCAGAT TTACAGACCA AAGGACAAAT ACCTGGATAC 
GGTTCAGGCT TTGGCTCAAG CAGCATGGAT TATGGCATGG TGGGAGGGAA AGAAGCTGGA 
ACAGAATCCC GCTTTAAACA GTGGACTATG ATGGAAGGGC TGCCCTCTGT GGCTTCACAA 
GATGCCAATA TGCACAAAAA TGGTGCAATA GTGCCCCCTG GAAAGGCTCG CGGAGGATCG 
CCCTACAACC AGTTCGACAT AATTCAGGGC GACCCACTAG GTGGCCATGC AGGCCCTGCT 
GGTGATAGTT GGTTACCTGC CAAATCTCCA CCGACAAACA AGATCGGAAG CAAATCCAGC 
AACGCCAGCT GGCCTCCAGA GTTCCAACCA GGAGTGCCAT GGAAAGGTAT ACAAAACATT 
GACCCTGAAT CTGACCCCTA CGTGACCCCT GGAAGTGTGC TGGGGGGGAC AGCCACATCT 
CCCATTGTAG ATACTGACCA CCAACTTCTG CGGGACAACA CCACAGGGTC TAATTCTTCC 
CTCAACACCT CGCTGCCTTC ACCTGGTGCC TGGCCCTACA GTGCCTCTGA CAATTCCTTC 
ACCAACGTTC ATAGCACTTC AGCAAAATTC AGTGATTACA AATCAACATG GTCCCCAGAT 
CCCATAGGAC ACAATCCCAC TCATCTCTCC AACAAGATGT GGAAAAACCA TATTTCCTCA 
AGGAACACTA CAGGGCTGCC TCGCCCGCCT CCTGGCCTGA CCAACCCCAA ACCATCATCT 
CCATGGAACA GCACAGCACC CCGATCGGTC AGGGGCTGGG GGGCACAGGA CTCAAGGCTT 
GCCTCTGGTG AGAAGGATCA TCTGATAACG CCATTGATCA GACCTGAGGA GTGTGCATTT 
TACAGCACTA ATGGTTTGGA GGAGAGAAAG TTGCAGTTGC CTGCATCTAC TTGGAGTGAT 
GGTAGCTCAG TTCGTCCTAG CTACTGGCTG GTTCTTCACA ATCTCACTCC ACAGATCGAT 
GGGTCAACCT TGAGGACGAT CTGCATGCAG CATGGTCCAC TGCTGACATT CCATCTGAAC 
CTGACCCAGG GCACCGCTCT CATCCGATAC AACACCAAAC AGGAGGCGGC CAAGGCCCAG 
ACTGCACTGC ACATGTGTGT GTTGGGAAAC ACTACCATCC TTGCTGAGTT TGCCACAGAT 
GAAGAGGTTA GTCGCTTTTT GACACAAGCT CAGCCCCCTA CACCTGCAGC AACCCCAAGT 
GCACCCACGG CAGGCTGGCA ATCCCTGGAG ACAGGACAGA ACCAGACAGA TCCAGTTGGA 
CCTGCTTTGA ATCTCTTTGG TGGGTCAACA GGGCTTGGGC AGTGGAGCAG TGGTGGCGGC 
GGCAGCAGTG GGGGTGATCT CACTGGCGCT TCGTTGTGGG GGCCCCCAAA CTATTCTTCA 
AGCTTGTGGG GGGTCCCAAG CGTAGAGGAT CCACACAGAA TGGGCAGCCC TGCTCCCTTA 
CTACCTGGAG ACCTTCTGGG AGGAGGGTCG GATTCAATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004937779.1
CDS	552..6233
Translation	MQTDEGEILEEGSPKMEHEDFVMEGHGKTPPPGEENKQEKEQEREEQLMEDKKRKKEDKKKKESAQKVTDQKTKVPEVTKPSLSQPVAASPIGNSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPPTAPGANPNNAQPVTGALLQSDSGTATDSTIGGAAASNYANSTWGPGASSSSGTNANPTHIWDKVIVDGSDMEEWPCIASKDAESSSENTTDNNSASNPGLEKNTLPGSTTSNKGKGSQCQSGSAGNECNLGAWKSDPKAKSVQSSNPATESNSGLSNWRNLTGQDRIGSGSGFSNFNPNSNPSAWPALVQEGNSRKGTSESDSGSSNAQISTVGQTSREQQSKMENAGVNFVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFEMGLGNPSRSTDTPSQSTGERKTGSVGSWGTARGSSGTDTVSGQSNSGNHGNNGKDREDSWKGASVQKPNGSRSDSWDNNNRSTGGGSWNFGPQHANESKWGEGNKLTSGVSQGEWKQLSGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNSQAPCWGRSSSSAGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEMPRSEGKSDKGTEGWESSATQTKNSGGWGDVPSQSNQIKSGWGELSTPTEWKDPKNTGGWNDYKNPNSSNWGGGRQEEKSSSSWNDSSSKDQGWGGRQPNQGWSSGKNGWGEEVDQAKNSNWESAGNKPASGWGEGGQNEIGTWGNGANTGSASKGGWDDCKRNSTWNETGRQPNSWNKQQQQQQQQQQETSGSWGPPPQTPSNGRPPNPNWNSGPQPAAPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPSSYNYKNVNLWDKNSQGGQAPREQSLPTPMTSKSQASVWSKSTPPAPDNGTSAWGEPNETSPGWGEVDDTGASTTGWGNAPSNAPNAMKSSSKSMQDGWGESDGPVTGTRHSSWEEEEEGGVWNTAGSQGSSSSHNSTSWGQGGKKTQMKCSLKGGNSDSWMNPISKQFSNMGLLSQTEDIPGNKMDLSVGGLPDKKFEVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLSNVGLGAIGSGLSPQNYAARQGGNHGLFGNSTAQSRGMHTPVQPLNPSPNIRAQVPPQFLSPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQQLLQSQRKLSQAVRQQQEQQIARMVSALQQQQQRQPGMKHSPSHPVGPKPHIDSMVPNPLNVGLSDLQTKGQIPGYGSGFGSSSMDYGMVGGKEAGTESRFKQWTMMEGLPSVASQDANMHKNGAIVPPGKARGGSPYNQFDIIQGDPLGGHAGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFSDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTGLPRPPPGLTNPKPSSPWNSTAPRSVRGWGAQDSRLASGEKDHLITPLIRPEECAFYSTNGLEERKLQLPASTWSDGSSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYNTKQEAAKAQTALHMCVLGNTTILAEFATDEEVSRFLTQAQPPTPAATPSAPTAGWQSLETGQNQTDPVGPALNLFGGSTGLGQWSSGGGGSSGGDLTGASLWGPPNYSSSLWGVPSVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_004937722.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004937722.3

ORF Insert Sequence:

ATGCAAACTG ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGATGGA ACATGAGGAT 
TTTGTAATGG AGGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAACAA ACAAGAGAAG 
GAGCAAGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAGGA GGATAAGAAA 
AAGAAGGAAT CTGCTCAGAA GGTCACAGAT CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGT GGCTGCCAGC CCAATTGGCA ACTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTGAAACGTG GGCAGCCCCC TCCACCATCC TGTATGCTCC TTGGGGGTGG AGCAGGGCCT 
CCTCCCCCTA CAGCACCAGG AGCAAACCCA AACAACGCAC AACCAGTGAC AGGAGCACTG 
CTGCAGAGCG ACAGTGGGAC TGCAACAGAT TCAACAATTG GAGGTGCTGC TGCTTCAAAT 
TATGCAAATT CCACTTGGGG TCCTGGAGCC TCCTCCAGCA GCGGCACCAA CGCCAACCCA 
ACTCACATCT GGGACAAGGT GATTGTAGAC GGGTCTGACA TGGAAGAGTG GCCTTGTATC 
GCCAGCAAAG ATGCTGAGTC TTCTTCCGAA AACACCACCG ATAACAACAG TGCCTCGAAC 
CCTGGCTTGG AGAAGAACAC TCTGCCAGGA AGCACCACTA GTAACAAAGG AAAAGGAAGC 
CAGTGCCAGT CTGGAAGCGC TGGAAATGAA TGTAATCTTG GGGCCTGGAA ATCTGATCCC 
AAGGCTAAAT CTGTTCAATC TTCCAACCCT GCTACCGAGA GTAACAGTGG ACTAAGTAAT 
TGGAGGAACT TGACTGGACA AGATAGAATT GGTTCTGGTT CTGGCTTCAG CAACTTTAAC 
CCAAATAGCA ACCCGTCTGC TTGGCCAGCA CTGGTCCAAG AGGGCAATTC TAGGAAAGGG 
ACTTCGGAAT CTGATAGTGG TAGCTCCAAT GCACAGATTA GCACAGTAGG TCAGACCTCT 
AGGGAACAGC AGTCAAAGAT GGAAAATGCG GGTGTTAACT TTGTCTCTGG CAGAGAACAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTTAAGT 
TCACCAAACC CTATGGAGAA TAAGGGAATG CCCTTTGAAA TGGGCTTGGG GAACCCTTCC 
AGGAGCACTG ACACCCCTTC ACAAAGCACT GGAGAAAGAA AGACTGGGAG TGTTGGATCT 
TGGGGTACAG CTAGGGGGTC TTCTGGAACT GACACAGTCT CTGGACAGAG CAATTCTGGA 
AACCATGGGA ACAATGGAAA GGATAGGGAG GACTCCTGGA AAGGAGCTTC TGTTCAAAAA 
CCTAATGGGT CAAGAAGCGA TTCTTGGGAT AACAATAACC GGTCTACGGG TGGTGGGTCT 
TGGAACTTTG GCCCTCAGCA TGCAAATGAA AGCAAATGGG GTGAAGGGAA CAAATTAACA 
TCTGGGGTCT CTCAGGGAGA ATGGAAACAG CTGTCTGGGT CTGATGAACT GAAGATTGGA 
GAATGGAGTG GTCCAAACCA ACCAAATTCT AGCACTGGAG CATGGGACAA TCAAAAAGGC 
CACCCTCTTC CTGAAAACCA AGGCAATTCC CAGGCTCCCT GTTGGGGAAG ATCTTCCAGC 
TCTGCAGGAA GTGAGGTTGG AGGTCAAAGC ACTGGAAGCA ACCACAAAGC AGGAAGTAGT 
GACAGTCACA ATTCTGGACG CCGATCATAC AGGCCTACGC ATCCTGATTG CCAGGCAGTC 
TTGCAGACTT TGCTGAGCAG GACTGATTTG GACCCCCGAG TGCTTTCAAA CACTGGCTGG 
GGCCAAACTC AAATTAAGCA AGATACCGTA TGGGATATTG AAGAGATGCC ACGGTCCGAG 
GGGAAGTCTG ATAAAGGAAC TGAGGGGTGG GAGAGCTCTG CCACACAGAC AAAGAACTCA 
GGGGGCTGGG GCGATGTACC CAGCCAAAGC AATCAAATCA AGTCTGGATG GGGTGAGCTC 
TCAACCCCAA CAGAATGGAA GGACCCCAAA AATACTGGAG GATGGAATGA CTATAAGAAC 
CCCAATTCGT CTAATTGGGG AGGGGGAAGA CAAGAAGAGA AATCGTCATC TTCTTGGAAT 
GACAGCTCTA GTAAGGATCA GGGGTGGGGT GGACGGCAGC CTAATCAAGG ATGGTCTTCT 
GGAAAGAATG GTTGGGGAGA GGAAGTTGAC CAAGCAAAAA ACAGCAACTG GGAAAGTGCG 
GGAAATAAGC CAGCATCTGG TTGGGGTGAA GGTGGGCAAA ATGAGATTGG AACTTGGGGT 
AACGGTGCAA ATACAGGCTC TGCTTCAAAG GGTGGTTGGG ATGATTGTAA AAGAAATTCA 
ACGTGGAACG AGACGGGTCG ACAGCCCAAC TCCTGGAACA AGCAGCAGCA GCAGCAACAG 
CAGCAACAGC AAGAGACTTC TGGTTCCTGG GGTCCACCGC CACAAACTCC AAGTAATGGG 
CGACCTCCAA ACCCAAACTG GAACAGCGGA CCACAACCAG CTGCCCCCAA AGACGAGGAG 
CCTAGTGGCT GGGAAGAACC TTCTCCACAG TCAATCAGTC GAAAAATGGA TATTGATGAT 
GGCACTTCAG CGTGGGGAGA TCCTAGCAGT TATAACTACA AGAATGTGAA CCTGTGGGAC 
AAGAACTCAC AAGGGGGACA GGCCCCACGG GAACAGAGCC TGCCTACTCC AATGACTAGC 
AAATCACAAG CATCAGTCTG GAGCAAAAGC ACTCCACCTG CTCCAGATAA TGGTACGTCC 
GCCTGGGGTG AGCCAAATGA AACCAGTCCC GGGTGGGGTG AAGTAGATGA TACAGGAGCA 
TCGACAACAG GCTGGGGGAA TGCACCTTCC AACGCCCCGA ATGCCATGAA ATCTAGTTCT 
AAATCTATGC AAGATGGCTG GGGAGAGAGT GATGGGCCAG TGACAGGAAC ACGTCATTCC 
AGCTGGGAAG AGGAGGAGGA GGGAGGAGTC TGGAACACAG CAGGCTCTCA GGGAAGCAGT 
TCCTCCCACA ACTCAACAAG CTGGGGGCAA GGAGGAAAGA AAACGCAGAT GAAGTGCTCG 
TTAAAAGGAG GAAATAGTGA TTCATGGATG AACCCTATAT CCAAGCAGTT TTCAAATATG 
GGCTTGCTAA GTCAAACTGA GGACATTCCA GGCAATAAGA TGGACTTGTC TGTAGGTGGT 
CTCCCAGATA AGAAGTTTGA GGTAGATAAA CGAGCCATGA ATCTCGGGGA TTTTAATGAC 
ATAATGAGAA AGGATCGATC TGGGTTCCGT CCACCTAATT CCAAAGACAT GGGAACCACA 
GATAGTGGGC CTTATTTTGA GAAGCTGACT TTGCCTTTCT CCAATCAAGA TGGGTGCCTT 
GGGGATGAGG CTCCCTGCTC TCCCTTCTCC CCTTCTCCCA GCTACAAGCT GTCTCCCTCT 
GGTTCCACAC TATCCAACGT CGGCCTTGGA GCAATCGGCT CAGGGCTCAG TCCCCAAAAC 
TACGCTGCTA GACAAGGTGG CAATCATGGT TTGTTTGGAA ACAGCACAGC ACAATCGAGG 
GGCATGCACA CACCAGTGCA GCCACTAAAT CCTTCCCCCA ATATCCGGGC GCAAGTGCCT 
CCCCAATTTC TTTCTCCCCA GGTTTCGGCC TCCATGCTCA AACAGTTCCC CAACAGTGGC 
TTGAATCCAG GTCTTTTCAA TGTGGGGCCC CAGCTTTCTC CACAACAGAT TGCCATGCTG 
AGCCAGCTTC CACAGATTCC CCAGTTTCAA TTAGCATGTC AGCTCCTCCT TCAGCAGCAG 
CAGCAGCAGC AGCAGCTGTT ACAGAGCCAG AGGAAGTTAT CTCAAGCTGT GCGACAGCAG 
CAGGAGCAAC AGATTGCTCG TATGGTGAGT GCCCTCCAGC AGCAGCAGCA GAGGCAGCCT 
GGCATGAAGC ATTCACCATC CCACCCTGTT GGGCCTAAGC CACATATAGA CAGCATGGTA 
CCCAATCCTT TGAATGTGGG TCTTTCAGAT TTACAGACCA AAGGACAAAT ACCTGGATAC 
GGTTCAGGCT TTGGCTCAAG CAGCATGGAT TATGGCATGG TGGGAGGGAA AGAAGCTGGA 
ACAGAATCCC GCTTTAAACA GTGGACTATG ATGGAAGGGC TGCCCTCTGT GGCTTCACAA 
GATGCCAATA TGCACAAAAA TGGTGCAATA GTGCCCCCTG GAAAGGCTCG CGGAGGATCG 
CCCTACAACC AGTTCGACAT AATTCAGGGC GACCCACTAG GTGGCCATGC AGGCCCTGCT 
GGTGATAGTT GGTTACCTGC CAAATCTCCA CCGACAAACA AGATCGGAAG CAAATCCAGC 
AACGCCAGCT GGCCTCCAGA GTTCCAACCA GGAGTGCCAT GGAAAGGTAT ACAAAACATT 
GACCCTGAAT CTGACCCCTA CGTGACCCCT GGAAGTGTGC TGGGGGGGAC AGCCACATCT 
CCCATTGTAG ATACTGACCA CCAACTTCTG CGGGACAACA CCACAGGGTC TAATTCTTCC 
CTCAACACCT CGCTGCCTTC ACCTGGTGCC TGGCCCTACA GTGCCTCTGA CAATTCCTTC 
ACCAACGTTC ATAGCACTTC AGCAAAATTC AGTGATTACA AATCAACATG GTCCCCAGAT 
CCCATAGGAC ACAATCCCAC TCATCTCTCC AACAAGATGT GGAAAAACCA TATTTCCTCA 
AGGAACACTA CAGGGCTGCC TCGCCCGCCT CCTGGCCTGA CCAACCCCAA ACCATCATCT 
CCATGGAACA GCACAGCACC CCGATCGGTC AGGGGCTGGG GGGCACAGGA CTCAAGGCTT 
GCCTCTGGTG AGAAGGATCA TCTGATAACG CCATTGATCA GACCTGAGGA GTGTGCATTT 
TACAGCACTA ATGGTTTGGA GGAGAGAAAG TTGCAGTTGC CTGCATCTAC TTGGAGTGAT 
GGTAGCTCAG TTCGTCCTAG CTACTGGCTG GTTCTTCACA ATCTCACTCC ACAGATCGAT 
GGGTCAACCT TGAGGACGAT CTGCATGCAG CATGGTCCAC TGCTGACATT CCATCTGAAC 
CTGACCCAGG GCACCGCTCT CATCCGATAC AACACCAAAC AGGAGGCGGC CAAGGCCCAG 
ACTGCACTGC ACATGTGTGT GTTGGGAAAC ACTACCATCC TTGCTGAGTT TGCCACAGAT 
GAAGAGGTTA GTCGCTTTTT GACACAAGCT CAGCCCCCTA CACCTGCAGC AACCCCAAGT 
GCACCCACGG CAGGCTGGCA ATCCCTGGAG ACAGGACAGA ACCAGACAGA TCCAGTTGGA 
CCTGCTTTGA ATCTCTTTGG TGGGTCAACA GGGCTTGGGC AGTGGAGCAG TGGTGGCGGC 
GGCAGCAGTG GGGGTGATCT CACTGGCGCT TCGTTGTGGG GGCCCCCAAA CTATTCTTCA 
AGCTTGTGGG GGGTCCCAAG CGTAGAGGAT CCACACAGAA TGGGCAGCCC TGCTCCCTTA 
CTACCTGGAG ACCTTCTGGG AGGAGGGTCG GATTCAATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_004937722.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004937779.1 Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X1, mRNA.

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CloneID	OGa01767
Clone ID Related Accession (Same CDS sequence)	XM_004937722.3 , XM_004937722.2
Accession Version	XM_004937722.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	trinucleotide repeat-containing gene 6B protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455714.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004937722.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACTG ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGATGGA ACATGAGGAT 
TTTGTAATGG AGGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAACAA ACAAGAGAAG 
GAGCAAGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAGGA GGATAAGAAA 
AAGAAGGAAT CTGCTCAGAA GGTCACAGAT CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGT GGCTGCCAGC CCAATTGGCA ACTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTGAAACGTG GGCAGCCCCC TCCACCATCC TGTATGCTCC TTGGGGGTGG AGCAGGGCCT 
CCTCCCCCTA CAGCACCAGG AGCAAACCCA AACAACGCAC AACCAGTGAC AGGAGCACTG 
CTGCAGAGCG ACAGTGGGAC TGCAACAGAT TCAACAATTG GAGGTGCTGC TGCTTCAAAT 
TATGCAAATT CCACTTGGGG TCCTGGAGCC TCCTCCAGCA GCGGCACCAA CGCCAACCCA 
ACTCACATCT GGGACAAGGT GATTGTAGAC GGGTCTGACA TGGAAGAGTG GCCTTGTATC 
GCCAGCAAAG ATGCTGAGTC TTCTTCCGAA AACACCACCG ATAACAACAG TGCCTCGAAC 
CCTGGCTTGG AGAAGAACAC TCTGCCAGGA AGCACCACTA GTAACAAAGG AAAAGGAAGC 
CAGTGCCAGT CTGGAAGCGC TGGAAATGAA TGTAATCTTG GGGCCTGGAA ATCTGATCCC 
AAGGCTAAAT CTGTTCAATC TTCCAACCCT GCTACCGAGA GTAACAGTGG ACTAAGTAAT 
TGGAGGAACT TGACTGGACA AGATAGAATT GGTTCTGGTT CTGGCTTCAG CAACTTTAAC 
CCAAATAGCA ACCCGTCTGC TTGGCCAGCA CTGGTCCAAG AGGGCAATTC TAGGAAAGGG 
ACTTCGGAAT CTGATAGTGG TAGCTCCAAT GCACAGATTA GCACAGTAGG TCAGACCTCT 
AGGGAACAGC AGTCAAAGAT GGAAAATGCG GGTGTTAACT TTGTCTCTGG CAGAGAACAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTTAAGT 
TCACCAAACC CTATGGAGAA TAAGGGAATG CCCTTTGAAA TGGGCTTGGG GAACCCTTCC 
AGGAGCACTG ACACCCCTTC ACAAAGCACT GGAGAAAGAA AGACTGGGAG TGTTGGATCT 
TGGGGTACAG CTAGGGGGTC TTCTGGAACT GACACAGTCT CTGGACAGAG CAATTCTGGA 
AACCATGGGA ACAATGGAAA GGATAGGGAG GACTCCTGGA AAGGAGCTTC TGTTCAAAAA 
CCTAATGGGT CAAGAAGCGA TTCTTGGGAT AACAATAACC GGTCTACGGG TGGTGGGTCT 
TGGAACTTTG GCCCTCAGCA TGCAAATGAA AGCAAATGGG GTGAAGGGAA CAAATTAACA 
TCTGGGGTCT CTCAGGGAGA ATGGAAACAG CTGTCTGGGT CTGATGAACT GAAGATTGGA 
GAATGGAGTG GTCCAAACCA ACCAAATTCT AGCACTGGAG CATGGGACAA TCAAAAAGGC 
CACCCTCTTC CTGAAAACCA AGGCAATTCC CAGGCTCCCT GTTGGGGAAG ATCTTCCAGC 
TCTGCAGGAA GTGAGGTTGG AGGTCAAAGC ACTGGAAGCA ACCACAAAGC AGGAAGTAGT 
GACAGTCACA ATTCTGGACG CCGATCATAC AGGCCTACGC ATCCTGATTG CCAGGCAGTC 
TTGCAGACTT TGCTGAGCAG GACTGATTTG GACCCCCGAG TGCTTTCAAA CACTGGCTGG 
GGCCAAACTC AAATTAAGCA AGATACCGTA TGGGATATTG AAGAGATGCC ACGGTCCGAG 
GGGAAGTCTG ATAAAGGAAC TGAGGGGTGG GAGAGCTCTG CCACACAGAC AAAGAACTCA 
GGGGGCTGGG GCGATGTACC CAGCCAAAGC AATCAAATCA AGTCTGGATG GGGTGAGCTC 
TCAACCCCAA CAGAATGGAA GGACCCCAAA AATACTGGAG GATGGAATGA CTATAAGAAC 
CCCAATTCGT CTAATTGGGG AGGGGGAAGA CAAGAAGAGA AATCGTCATC TTCTTGGAAT 
GACAGCTCTA GTAAGGATCA GGGGTGGGGT GGACGGCAGC CTAATCAAGG ATGGTCTTCT 
GGAAAGAATG GTTGGGGAGA GGAAGTTGAC CAAGCAAAAA ACAGCAACTG GGAAAGTGCG 
GGAAATAAGC CAGCATCTGG TTGGGGTGAA GGTGGGCAAA ATGAGATTGG AACTTGGGGT 
AACGGTGCAA ATACAGGCTC TGCTTCAAAG GGTGGTTGGG ATGATTGTAA AAGAAATTCA 
ACGTGGAACG AGACGGGTCG ACAGCCCAAC TCCTGGAACA AGCAGCAGCA GCAGCAACAG 
CAGCAACAGC AAGAGACTTC TGGTTCCTGG GGTCCACCGC CACAAACTCC AAGTAATGGG 
CGACCTCCAA ACCCAAACTG GAACAGCGGA CCACAACCAG CTGCCCCCAA AGACGAGGAG 
CCTAGTGGCT GGGAAGAACC TTCTCCACAG TCAATCAGTC GAAAAATGGA TATTGATGAT 
GGCACTTCAG CGTGGGGAGA TCCTAGCAGT TATAACTACA AGAATGTGAA CCTGTGGGAC 
AAGAACTCAC AAGGGGGACA GGCCCCACGG GAACAGAGCC TGCCTACTCC AATGACTAGC 
AAATCACAAG CATCAGTCTG GAGCAAAAGC ACTCCACCTG CTCCAGATAA TGGTACGTCC 
GCCTGGGGTG AGCCAAATGA AACCAGTCCC GGGTGGGGTG AAGTAGATGA TACAGGAGCA 
TCGACAACAG GCTGGGGGAA TGCACCTTCC AACGCCCCGA ATGCCATGAA ATCTAGTTCT 
AAATCTATGC AAGATGGCTG GGGAGAGAGT GATGGGCCAG TGACAGGAAC ACGTCATTCC 
AGCTGGGAAG AGGAGGAGGA GGGAGGAGTC TGGAACACAG CAGGCTCTCA GGGAAGCAGT 
TCCTCCCACA ACTCAACAAG CTGGGGGCAA GGAGGAAAGA AAACGCAGAT GAAGTGCTCG 
TTAAAAGGAG GAAATAGTGA TTCATGGATG AACCCTATAT CCAAGCAGTT TTCAAATATG 
GGCTTGCTAA GTCAAACTGA GGACATTCCA GGCAATAAGA TGGACTTGTC TGTAGGTGGT 
CTCCCAGATA AGAAGTTTGA GGTAGATAAA CGAGCCATGA ATCTCGGGGA TTTTAATGAC 
ATAATGAGAA AGGATCGATC TGGGTTCCGT CCACCTAATT CCAAAGACAT GGGAACCACA 
GATAGTGGGC CTTATTTTGA GAAGCTGACT TTGCCTTTCT CCAATCAAGA TGGGTGCCTT 
GGGGATGAGG CTCCCTGCTC TCCCTTCTCC CCTTCTCCCA GCTACAAGCT GTCTCCCTCT 
GGTTCCACAC TATCCAACGT CGGCCTTGGA GCAATCGGCT CAGGGCTCAG TCCCCAAAAC 
TACGCTGCTA GACAAGGTGG CAATCATGGT TTGTTTGGAA ACAGCACAGC ACAATCGAGG 
GGCATGCACA CACCAGTGCA GCCACTAAAT CCTTCCCCCA ATATCCGGGC GCAAGTGCCT 
CCCCAATTTC TTTCTCCCCA GGTTTCGGCC TCCATGCTCA AACAGTTCCC CAACAGTGGC 
TTGAATCCAG GTCTTTTCAA TGTGGGGCCC CAGCTTTCTC CACAACAGAT TGCCATGCTG 
AGCCAGCTTC CACAGATTCC CCAGTTTCAA TTAGCATGTC AGCTCCTCCT TCAGCAGCAG 
CAGCAGCAGC AGCAGCTGTT ACAGAGCCAG AGGAAGTTAT CTCAAGCTGT GCGACAGCAG 
CAGGAGCAAC AGATTGCTCG TATGGTGAGT GCCCTCCAGC AGCAGCAGCA GAGGCAGCCT 
GGCATGAAGC ATTCACCATC CCACCCTGTT GGGCCTAAGC CACATATAGA CAGCATGGTA 
CCCAATCCTT TGAATGTGGG TCTTTCAGAT TTACAGACCA AAGGACAAAT ACCTGGATAC 
GGTTCAGGCT TTGGCTCAAG CAGCATGGAT TATGGCATGG TGGGAGGGAA AGAAGCTGGA 
ACAGAATCCC GCTTTAAACA GTGGACTATG ATGGAAGGGC TGCCCTCTGT GGCTTCACAA 
GATGCCAATA TGCACAAAAA TGGTGCAATA GTGCCCCCTG GAAAGGCTCG CGGAGGATCG 
CCCTACAACC AGTTCGACAT AATTCAGGGC GACCCACTAG GTGGCCATGC AGGCCCTGCT 
GGTGATAGTT GGTTACCTGC CAAATCTCCA CCGACAAACA AGATCGGAAG CAAATCCAGC 
AACGCCAGCT GGCCTCCAGA GTTCCAACCA GGAGTGCCAT GGAAAGGTAT ACAAAACATT 
GACCCTGAAT CTGACCCCTA CGTGACCCCT GGAAGTGTGC TGGGGGGGAC AGCCACATCT 
CCCATTGTAG ATACTGACCA CCAACTTCTG CGGGACAACA CCACAGGGTC TAATTCTTCC 
CTCAACACCT CGCTGCCTTC ACCTGGTGCC TGGCCCTACA GTGCCTCTGA CAATTCCTTC 
ACCAACGTTC ATAGCACTTC AGCAAAATTC AGTGATTACA AATCAACATG GTCCCCAGAT 
CCCATAGGAC ACAATCCCAC TCATCTCTCC AACAAGATGT GGAAAAACCA TATTTCCTCA 
AGGAACACTA CAGGGCTGCC TCGCCCGCCT CCTGGCCTGA CCAACCCCAA ACCATCATCT 
CCATGGAACA GCACAGCACC CCGATCGGTC AGGGGCTGGG GGGCACAGGA CTCAAGGCTT 
GCCTCTGGTG AGAAGGATCA TCTGATAACG CCATTGATCA GACCTGAGGA GTGTGCATTT 
TACAGCACTA ATGGTTTGGA GGAGAGAAAG TTGCAGTTGC CTGCATCTAC TTGGAGTGAT 
GGTAGCTCAG TTCGTCCTAG CTACTGGCTG GTTCTTCACA ATCTCACTCC ACAGATCGAT 
GGGTCAACCT TGAGGACGAT CTGCATGCAG CATGGTCCAC TGCTGACATT CCATCTGAAC 
CTGACCCAGG GCACCGCTCT CATCCGATAC AACACCAAAC AGGAGGCGGC CAAGGCCCAG 
ACTGCACTGC ACATGTGTGT GTTGGGAAAC ACTACCATCC TTGCTGAGTT TGCCACAGAT 
GAAGAGGTTA GTCGCTTTTT GACACAAGCT CAGCCCCCTA CACCTGCAGC AACCCCAAGT 
GCACCCACGG CAGGCTGGCA ATCCCTGGAG ACAGGACAGA ACCAGACAGA TCCAGTTGGA 
CCTGCTTTGA ATCTCTTTGG TGGGTCAACA GGGCTTGGGC AGTGGAGCAG TGGTGGCGGC 
GGCAGCAGTG GGGGTGATCT CACTGGCGCT TCGTTGTGGG GGCCCCCAAA CTATTCTTCA 
AGCTTGTGGG GGGTCCCAAG CGTAGAGGAT CCACACAGAA TGGGCAGCCC TGCTCCCTTA 
CTACCTGGAG ACCTTCTGGG AGGAGGGTCG GATTCAATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004937779.1
CDS	541..6222
Translation	MQTDEGEILEEGSPKMEHEDFVMEGHGKTPPPGEENKQEKEQEREEQLMEDKKRKKEDKKKKESAQKVTDQKTKVPEVTKPSLSQPVAASPIGNSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPPTAPGANPNNAQPVTGALLQSDSGTATDSTIGGAAASNYANSTWGPGASSSSGTNANPTHIWDKVIVDGSDMEEWPCIASKDAESSSENTTDNNSASNPGLEKNTLPGSTTSNKGKGSQCQSGSAGNECNLGAWKSDPKAKSVQSSNPATESNSGLSNWRNLTGQDRIGSGSGFSNFNPNSNPSAWPALVQEGNSRKGTSESDSGSSNAQISTVGQTSREQQSKMENAGVNFVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFEMGLGNPSRSTDTPSQSTGERKTGSVGSWGTARGSSGTDTVSGQSNSGNHGNNGKDREDSWKGASVQKPNGSRSDSWDNNNRSTGGGSWNFGPQHANESKWGEGNKLTSGVSQGEWKQLSGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNSQAPCWGRSSSSAGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEMPRSEGKSDKGTEGWESSATQTKNSGGWGDVPSQSNQIKSGWGELSTPTEWKDPKNTGGWNDYKNPNSSNWGGGRQEEKSSSSWNDSSSKDQGWGGRQPNQGWSSGKNGWGEEVDQAKNSNWESAGNKPASGWGEGGQNEIGTWGNGANTGSASKGGWDDCKRNSTWNETGRQPNSWNKQQQQQQQQQQETSGSWGPPPQTPSNGRPPNPNWNSGPQPAAPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPSSYNYKNVNLWDKNSQGGQAPREQSLPTPMTSKSQASVWSKSTPPAPDNGTSAWGEPNETSPGWGEVDDTGASTTGWGNAPSNAPNAMKSSSKSMQDGWGESDGPVTGTRHSSWEEEEEGGVWNTAGSQGSSSSHNSTSWGQGGKKTQMKCSLKGGNSDSWMNPISKQFSNMGLLSQTEDIPGNKMDLSVGGLPDKKFEVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLSNVGLGAIGSGLSPQNYAARQGGNHGLFGNSTAQSRGMHTPVQPLNPSPNIRAQVPPQFLSPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQQLLQSQRKLSQAVRQQQEQQIARMVSALQQQQQRQPGMKHSPSHPVGPKPHIDSMVPNPLNVGLSDLQTKGQIPGYGSGFGSSSMDYGMVGGKEAGTESRFKQWTMMEGLPSVASQDANMHKNGAIVPPGKARGGSPYNQFDIIQGDPLGGHAGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFSDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTGLPRPPPGLTNPKPSSPWNSTAPRSVRGWGAQDSRLASGEKDHLITPLIRPEECAFYSTNGLEERKLQLPASTWSDGSSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYNTKQEAAKAQTALHMCVLGNTTILAEFATDEEVSRFLTQAQPPTPAATPSAPTAGWQSLETGQNQTDPVGPALNLFGGSTGLGQWSSGGGGSSGGDLTGASLWGPPNYSSSLWGVPSVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_004937722.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004937722.2

ORF Insert Sequence:

ATGCAAACTG ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGATGGA ACATGAGGAT 
TTTGTAATGG AGGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAACAA ACAAGAGAAG 
GAGCAAGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAGGA GGATAAGAAA 
AAGAAGGAAT CTGCTCAGAA GGTCACAGAT CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGT GGCTGCCAGC CCAATTGGCA ACTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTGAAACGTG GGCAGCCCCC TCCACCATCC TGTATGCTCC TTGGGGGTGG AGCAGGGCCT 
CCTCCCCCTA CAGCACCAGG AGCAAACCCA AACAACGCAC AACCAGTGAC AGGAGCACTG 
CTGCAGAGCG ACAGTGGGAC TGCAACAGAT TCAACAATTG GAGGTGCTGC TGCTTCAAAT 
TATGCAAATT CCACTTGGGG TCCTGGAGCC TCCTCCAGCA GCGGCACCAA CGCCAACCCA 
ACTCACATCT GGGACAAGGT GATTGTAGAC GGGTCTGACA TGGAAGAGTG GCCTTGTATC 
GCCAGCAAAG ATGCTGAGTC TTCTTCCGAA AACACCACCG ATAACAACAG TGCCTCGAAC 
CCTGGCTTGG AGAAGAACAC TCTGCCAGGA AGCACCACTA GTAACAAAGG AAAAGGAAGC 
CAGTGCCAGT CTGGAAGCGC TGGAAATGAA TGTAATCTTG GGGCCTGGAA ATCTGATCCC 
AAGGCTAAAT CTGTTCAATC TTCCAACCCT GCTACCGAGA GTAACAGTGG ACTAAGTAAT 
TGGAGGAACT TGACTGGACA AGATAGAATT GGTTCTGGTT CTGGCTTCAG CAACTTTAAC 
CCAAATAGCA ACCCGTCTGC TTGGCCAGCA CTGGTCCAAG AGGGCAATTC TAGGAAAGGG 
ACTTCGGAAT CTGATAGTGG TAGCTCCAAT GCACAGATTA GCACAGTAGG TCAGACCTCT 
AGGGAACAGC AGTCAAAGAT GGAAAATGCG GGTGTTAACT TTGTCTCTGG CAGAGAACAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTTAAGT 
TCACCAAACC CTATGGAGAA TAAGGGAATG CCCTTTGAAA TGGGCTTGGG GAACCCTTCC 
AGGAGCACTG ACACCCCTTC ACAAAGCACT GGAGAAAGAA AGACTGGGAG TGTTGGATCT 
TGGGGTACAG CTAGGGGGTC TTCTGGAACT GACACAGTCT CTGGACAGAG CAATTCTGGA 
AACCATGGGA ACAATGGAAA GGATAGGGAG GACTCCTGGA AAGGAGCTTC TGTTCAAAAA 
CCTAATGGGT CAAGAAGCGA TTCTTGGGAT AACAATAACC GGTCTACGGG TGGTGGGTCT 
TGGAACTTTG GCCCTCAGCA TGCAAATGAA AGCAAATGGG GTGAAGGGAA CAAATTAACA 
TCTGGGGTCT CTCAGGGAGA ATGGAAACAG CTGTCTGGGT CTGATGAACT GAAGATTGGA 
GAATGGAGTG GTCCAAACCA ACCAAATTCT AGCACTGGAG CATGGGACAA TCAAAAAGGC 
CACCCTCTTC CTGAAAACCA AGGCAATTCC CAGGCTCCCT GTTGGGGAAG ATCTTCCAGC 
TCTGCAGGAA GTGAGGTTGG AGGTCAAAGC ACTGGAAGCA ACCACAAAGC AGGAAGTAGT 
GACAGTCACA ATTCTGGACG CCGATCATAC AGGCCTACGC ATCCTGATTG CCAGGCAGTC 
TTGCAGACTT TGCTGAGCAG GACTGATTTG GACCCCCGAG TGCTTTCAAA CACTGGCTGG 
GGCCAAACTC AAATTAAGCA AGATACCGTA TGGGATATTG AAGAGATGCC ACGGTCCGAG 
GGGAAGTCTG ATAAAGGAAC TGAGGGGTGG GAGAGCTCTG CCACACAGAC AAAGAACTCA 
GGGGGCTGGG GCGATGTACC CAGCCAAAGC AATCAAATCA AGTCTGGATG GGGTGAGCTC 
TCAACCCCAA CAGAATGGAA GGACCCCAAA AATACTGGAG GATGGAATGA CTATAAGAAC 
CCCAATTCGT CTAATTGGGG AGGGGGAAGA CAAGAAGAGA AATCGTCATC TTCTTGGAAT 
GACAGCTCTA GTAAGGATCA GGGGTGGGGT GGACGGCAGC CTAATCAAGG ATGGTCTTCT 
GGAAAGAATG GTTGGGGAGA GGAAGTTGAC CAAGCAAAAA ACAGCAACTG GGAAAGTGCG 
GGAAATAAGC CAGCATCTGG TTGGGGTGAA GGTGGGCAAA ATGAGATTGG AACTTGGGGT 
AACGGTGCAA ATACAGGCTC TGCTTCAAAG GGTGGTTGGG ATGATTGTAA AAGAAATTCA 
ACGTGGAACG AGACGGGTCG ACAGCCCAAC TCCTGGAACA AGCAGCAGCA GCAGCAACAG 
CAGCAACAGC AAGAGACTTC TGGTTCCTGG GGTCCACCGC CACAAACTCC AAGTAATGGG 
CGACCTCCAA ACCCAAACTG GAACAGCGGA CCACAACCAG CTGCCCCCAA AGACGAGGAG 
CCTAGTGGCT GGGAAGAACC TTCTCCACAG TCAATCAGTC GAAAAATGGA TATTGATGAT 
GGCACTTCAG CGTGGGGAGA TCCTAGCAGT TATAACTACA AGAATGTGAA CCTGTGGGAC 
AAGAACTCAC AAGGGGGACA GGCCCCACGG GAACAGAGCC TGCCTACTCC AATGACTAGC 
AAATCACAAG CATCAGTCTG GAGCAAAAGC ACTCCACCTG CTCCAGATAA TGGTACGTCC 
GCCTGGGGTG AGCCAAATGA AACCAGTCCC GGGTGGGGTG AAGTAGATGA TACAGGAGCA 
TCGACAACAG GCTGGGGGAA TGCACCTTCC AACGCCCCGA ATGCCATGAA ATCTAGTTCT 
AAATCTATGC AAGATGGCTG GGGAGAGAGT GATGGGCCAG TGACAGGAAC ACGTCATTCC 
AGCTGGGAAG AGGAGGAGGA GGGAGGAGTC TGGAACACAG CAGGCTCTCA GGGAAGCAGT 
TCCTCCCACA ACTCAACAAG CTGGGGGCAA GGAGGAAAGA AAACGCAGAT GAAGTGCTCG 
TTAAAAGGAG GAAATAGTGA TTCATGGATG AACCCTATAT CCAAGCAGTT TTCAAATATG 
GGCTTGCTAA GTCAAACTGA GGACATTCCA GGCAATAAGA TGGACTTGTC TGTAGGTGGT 
CTCCCAGATA AGAAGTTTGA GGTAGATAAA CGAGCCATGA ATCTCGGGGA TTTTAATGAC 
ATAATGAGAA AGGATCGATC TGGGTTCCGT CCACCTAATT CCAAAGACAT GGGAACCACA 
GATAGTGGGC CTTATTTTGA GAAGCTGACT TTGCCTTTCT CCAATCAAGA TGGGTGCCTT 
GGGGATGAGG CTCCCTGCTC TCCCTTCTCC CCTTCTCCCA GCTACAAGCT GTCTCCCTCT 
GGTTCCACAC TATCCAACGT CGGCCTTGGA GCAATCGGCT CAGGGCTCAG TCCCCAAAAC 
TACGCTGCTA GACAAGGTGG CAATCATGGT TTGTTTGGAA ACAGCACAGC ACAATCGAGG 
GGCATGCACA CACCAGTGCA GCCACTAAAT CCTTCCCCCA ATATCCGGGC GCAAGTGCCT 
CCCCAATTTC TTTCTCCCCA GGTTTCGGCC TCCATGCTCA AACAGTTCCC CAACAGTGGC 
TTGAATCCAG GTCTTTTCAA TGTGGGGCCC CAGCTTTCTC CACAACAGAT TGCCATGCTG 
AGCCAGCTTC CACAGATTCC CCAGTTTCAA TTAGCATGTC AGCTCCTCCT TCAGCAGCAG 
CAGCAGCAGC AGCAGCTGTT ACAGAGCCAG AGGAAGTTAT CTCAAGCTGT GCGACAGCAG 
CAGGAGCAAC AGATTGCTCG TATGGTGAGT GCCCTCCAGC AGCAGCAGCA GAGGCAGCCT 
GGCATGAAGC ATTCACCATC CCACCCTGTT GGGCCTAAGC CACATATAGA CAGCATGGTA 
CCCAATCCTT TGAATGTGGG TCTTTCAGAT TTACAGACCA AAGGACAAAT ACCTGGATAC 
GGTTCAGGCT TTGGCTCAAG CAGCATGGAT TATGGCATGG TGGGAGGGAA AGAAGCTGGA 
ACAGAATCCC GCTTTAAACA GTGGACTATG ATGGAAGGGC TGCCCTCTGT GGCTTCACAA 
GATGCCAATA TGCACAAAAA TGGTGCAATA GTGCCCCCTG GAAAGGCTCG CGGAGGATCG 
CCCTACAACC AGTTCGACAT AATTCAGGGC GACCCACTAG GTGGCCATGC AGGCCCTGCT 
GGTGATAGTT GGTTACCTGC CAAATCTCCA CCGACAAACA AGATCGGAAG CAAATCCAGC 
AACGCCAGCT GGCCTCCAGA GTTCCAACCA GGAGTGCCAT GGAAAGGTAT ACAAAACATT 
GACCCTGAAT CTGACCCCTA CGTGACCCCT GGAAGTGTGC TGGGGGGGAC AGCCACATCT 
CCCATTGTAG ATACTGACCA CCAACTTCTG CGGGACAACA CCACAGGGTC TAATTCTTCC 
CTCAACACCT CGCTGCCTTC ACCTGGTGCC TGGCCCTACA GTGCCTCTGA CAATTCCTTC 
ACCAACGTTC ATAGCACTTC AGCAAAATTC AGTGATTACA AATCAACATG GTCCCCAGAT 
CCCATAGGAC ACAATCCCAC TCATCTCTCC AACAAGATGT GGAAAAACCA TATTTCCTCA 
AGGAACACTA CAGGGCTGCC TCGCCCGCCT CCTGGCCTGA CCAACCCCAA ACCATCATCT 
CCATGGAACA GCACAGCACC CCGATCGGTC AGGGGCTGGG GGGCACAGGA CTCAAGGCTT 
GCCTCTGGTG AGAAGGATCA TCTGATAACG CCATTGATCA GACCTGAGGA GTGTGCATTT 
TACAGCACTA ATGGTTTGGA GGAGAGAAAG TTGCAGTTGC CTGCATCTAC TTGGAGTGAT 
GGTAGCTCAG TTCGTCCTAG CTACTGGCTG GTTCTTCACA ATCTCACTCC ACAGATCGAT 
GGGTCAACCT TGAGGACGAT CTGCATGCAG CATGGTCCAC TGCTGACATT CCATCTGAAC 
CTGACCCAGG GCACCGCTCT CATCCGATAC AACACCAAAC AGGAGGCGGC CAAGGCCCAG 
ACTGCACTGC ACATGTGTGT GTTGGGAAAC ACTACCATCC TTGCTGAGTT TGCCACAGAT 
GAAGAGGTTA GTCGCTTTTT GACACAAGCT CAGCCCCCTA CACCTGCAGC AACCCCAAGT 
GCACCCACGG CAGGCTGGCA ATCCCTGGAG ACAGGACAGA ACCAGACAGA TCCAGTTGGA 
CCTGCTTTGA ATCTCTTTGG TGGGTCAACA GGGCTTGGGC AGTGGAGCAG TGGTGGCGGC 
GGCAGCAGTG GGGGTGATCT CACTGGCGCT TCGTTGTGGG GGCCCCCAAA CTATTCTTCA 
AGCTTGTGGG GGGTCCCAAG CGTAGAGGAT CCACACAGAA TGGGCAGCCC TGCTCCCTTA 
CTACCTGGAG ACCTTCTGGG AGGAGGGTCG GATTCAATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_025155217.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025010985.1 Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X10, mRNA.

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CloneID	OGa01768
Clone ID Related Accession (Same CDS sequence)	XM_025155217.1 , XM_004937723.2 , XM_004937723.3
Accession Version	XM_025155217.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	trinucleotide repeat-containing gene 6B protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGAGAGA AGGAGCAAGA AAGGGAAGAA 
CAGTTAATGG AAGACAAGAA AAGGAAGAAG GAGGATAAGA AAAAGAAGGA ATCTGCTCAG 
AAGGTCACAG ATCAAAAAAC CAAAGTGCCC GAAGTGACGA AACCAAGTTT AAGCCAACCA 
GTGGCTGCCA GCCCAATTGG CAACTCTCCA TCGCCACCAG TCAATGGTGG CAACAATGCC 
AAAAGGGTGG CAGTGCCGAA CGGACAACCG CCAAGCGCCG CCCGCTACAT GCCTCGGGAG 
GTGCCGCCGC GATTCCGTTG CCAGCAGGAC CACAAAGTGT TACTGAAACG TGGGCAGCCC 
CCTCCACCAT CCTGTATGCT CCTTGGGGGT GGAGCAGGGC CTCCTCCCCC TACAGCACCA 
GGAGCAAACC CAAACAACGC ACAACCAGTG ACAGGAGCAC TGCTGCAGAG CGACAGTGGG 
ACTGCAACAG ATTCAACAAT TGGAGGTGCT GCTGCTTCAA ATTATGCAAA TTCCACTTGG 
GGTCCTGGAG CCTCCTCCAG CAGCGGCACC AACGCCAACC CAACTCACAT CTGGGACAAG 
GTGATTGTAG ACGGGTCTGA CATGGAAGAG TGGCCTTGTA TCGCCAGCAA AGATGCTGAG 
TCTTCTTCCG AAAACACCAC CGATAACAAC AGTGCCTCGA ACCCTGGCTT GGAGAAGAAC 
ACTCTGCCAG GAAGCACCAC TAGTAACAAA GGAAAAGGAA GCCAGTGCCA GTCTGGAAGC 
GCTGGAAATG AATGTAATCT TGGGGCCTGG AAATCTGATC CCAAGGCTAA ATCTGTTCAA 
TCTTCCAACC CTGCTACCGA GAGTAACAGT GGACTAAGTA ATTGGAGGAA CTTGACTGGA 
CAAGATAGAA TTGGTTCTGG TTCTGGCTTC AGCAACTTTA ACCCAAATAG CAACCCGTCT 
GCTTGGCCAG CACTGGTCCA AGAGGGCAAT TCTAGGAAAG GGACTTCGGA ATCTGATAGT 
GGTAGCTCCA ATGCACAGAT TAGCACAGTA GGTCAGACCT CTAGGGAACA GCAGTCAAAG 
ATGGAAAATG CGGGTGTTAA CTTTGTCTCT GGCAGAGAAC AGGCTCAAAT TCATAACACT 
GATGGACCAA AAAATGGAAA CACTAACTCC TTGAACTTAA GTTCACCAAA CCCTATGGAG 
AATAAGGGAA TGCCCTTTGA AATGGGCTTG GGGAACCCTT CCAGGAGCAC TGACACCCCT 
TCACAAAGCA CTGGAGAAAG AAAGACTGGG AGTGTTGGAT CTTGGGGTAC AGCTAGGGGG 
TCTTCTGGAA CTGACACAGT CTCTGGACAG AGCAATTCTG GAAACCATGG GAACAATGGA 
AAGGATAGGG AGGACTCCTG GAAAGGAGCT TCTGTTCAAA AACCTAATGG GTCAAGAAGC 
GATTCTTGGG ATAACAATAA CCGGTCTACG GGTGGTGGGT CTTGGAACTT TGGCCCTCAG 
CATGCAAATG AAAGCAAATG GGGTGAAGGG AACAAATTAA CATCTGGGGT CTCTCAGGGA 
GAATGGAAAC AGCTGTCTGG GTCTGATGAA CTGAAGATTG GAGAATGGAG TGGTCCAAAC 
CAACCAAATT CTAGCACTGG AGCATGGGAC AATCAAAAAG GCCACCCTCT TCCTGAAAAC 
CAAGGCAATT CCCAGGCTCC CTGTTGGGGA AGATCTTCCA GCTCTGCAGG AAGTGAGGTT 
GGAGGTCAAA GCACTGGAAG CAACCACAAA GCAGGAAGTA GTGACAGTCA CAATTCTGGA 
CGCCGATCAT ACAGGCCTAC GCATCCTGAT TGCCAGGCAG TCTTGCAGAC TTTGCTGAGC 
AGGACTGATT TGGACCCCCG AGTGCTTTCA AACACTGGCT GGGGCCAAAC TCAAATTAAG 
CAAGATACCG TATGGGATAT TGAAGAGATG CCACGGTCCG AGGGGAAGTC TGATAAAGGA 
ACTGAGGGGT GGGAGAGCTC TGCCACACAG ACAAAGAACT CAGGGGGCTG GGGCGATGTA 
CCCAGCCAAA GCAATCAAAT CAAGTCTGGA TGGGGTGAGC TCTCAACCCC AACAGAATGG 
AAGGACCCCA AAAATACTGG AGGATGGAAT GACTATAAGA ACCCCAATTC GTCTAATTGG 
GGAGGGGGAA GACAAGAAGA GAAATCGTCA TCTTCTTGGA ATGACAGCTC TAGTAAGGAT 
CAGGGGTGGG GTGGACGGCA GCCTAATCAA GGATGGTCTT CTGGAAAGAA TGGTTGGGGA 
GAGGAAGTTG ACCAAGCAAA AAACAGCAAC TGGGAAAGTG CGGGAAATAA GCCAGCATCT 
GGTTGGGGTG AAGGTGGGCA AAATGAGATT GGAACTTGGG GTAACGGTGC AAATACAGGC 
TCTGCTTCAA AGGGTGGTTG GGATGATTGT AAAAGAAATT CAACGTGGAA CGAGACGGGT 
CGACAGCCCA ACTCCTGGAA CAAGCAGCAG CAGCAGCAAC AGCAGCAACA GCAAGAGACT 
TCTGGTTCCT GGGGTCCACC GCCACAAACT CCAAGTAATG GGCGACCTCC AAACCCAAAC 
TGGAACAGCG GACCACAACC AGCTGCCCCC AAAGACGAGG AGCCTAGTGG CTGGGAAGAA 
CCTTCTCCAC AGTCAATCAG TCGAAAAATG GATATTGATG ATGGCACTTC AGCGTGGGGA 
GATCCTAGCA GTTATAACTA CAAGAATGTG AACCTGTGGG ACAAGAACTC ACAAGGGGGA 
CAGGCCCCAC GGGAACAGAG CCTGCCTACT CCAATGACTA GCAAATCACA AGCATCAGTC 
TGGAGCAAAA GCACTCCACC TGCTCCAGAT AATGGTACGT CCGCCTGGGG TGAGCCAAAT 
GAAACCAGTC CCGGGTGGGG TGAAGTAGAT GATACAGGAG CATCGACAAC AGGCTGGGGG 
AATGCACCTT CCAACGCCCC GAATGCCATG AAATCTAGTT CTAAATCTAT GCAAGATGGC 
TGGGGAGAGA GTGATGGGCC AGTGACAGGA ACACGTCATT CCAGCTGGGA AGAGGAGGAG 
GAGGGAGGAG TCTGGAACAC AGCAGGCTCT CAGGGAAGCA GTTCCTCCCA CAACTCAACA 
AGCTGGGGGC AAGGAGGAAA GAAAACGCAG ATGAAGTGCT CGTTAAAAGG AGGAAATAGT 
GATTCATGGA TGAACCCTAT ATCCAAGCAG TTTTCAAATA TGGGCTTGCT AAGTCAAACT 
GAGGACATTC CAGGCAATAA GATGGACTTG TCTGTAGGTG GTCTCCCAGA TAAGAAGTTT 
GAGGTAGATA AACGAGCCAT GAATCTCGGG GATTTTAATG ACATAATGAG AAAGGATCGA 
TCTGGGTTCC GTCCACCTAA TTCCAAAGAC ATGGGAACCA CAGATAGTGG GCCTTATTTT 
GAGAAGCTGA CTTTGCCTTT CTCCAATCAA GATGGGTGCC TTGGGGATGA GGCTCCCTGC 
TCTCCCTTCT CCCCTTCTCC CAGCTACAAG CTGTCTCCCT CTGGTTCCAC ACTATCCAAC 
GTCGGCCTTG GAGCAATCGG CTCAGGGCTC AGTCCCCAAA ACTACGCTGC TAGACAAGGT 
GGCAATCATG GTTTGTTTGG AAACAGCACA GCACAATCGA GGGGCATGCA CACACCAGTG 
CAGCCACTAA ATCCTTCCCC CAATATCCGG GCGCAAGTGC CTCCCCAATT TCTTTCTCCC 
CAGGTTTCGG CCTCCATGCT CAAACAGTTC CCCAACAGTG GCTTGAATCC AGGTCTTTTC 
AATGTGGGGC CCCAGCTTTC TCCACAACAG ATTGCCATGC TGAGCCAGCT TCCACAGATT 
CCCCAGTTTC AATTAGCATG TCAGCTCCTC CTTCAGCAGC AGCAGCAGCA GCAGCAGCTG 
TTACAGAGCC AGAGGAAGTT ATCTCAAGCT GTGCGACAGC AGCAGGAGCA ACAGATTGCT 
CGTATGGTGA GTGCCCTCCA GCAGCAGCAG CAGAGGCAGC CTGGCATGAA GCATTCACCA 
TCCCACCCTG TTGGGCCTAA GCCACATATA GACAGCATGG TACCCAATCC TTTGAATGTG 
GGTCTTTCAG ATTTACAGAC CAAAGGACAA ATACCTGGAT ACGGTTCAGG CTTTGGCTCA 
AGCAGCATGG ATTATGGCAT GGTGGGAGGG AAAGAAGCTG GAACAGAATC CCGCTTTAAA 
CAGTGGACTA TGATGGAAGG GCTGCCCTCT GTGGCTTCAC AAGATGCCAA TATGCACAAA 
AATGGTGCAA TAGTGCCCCC TGGAAAGGCT CGCGGAGGAT CGCCCTACAA CCAGTTCGAC 
ATAATTCAGG GCGACCCACT AGGTGGCCAT GCAGGCCCTG CTGGTGATAG TTGGTTACCT 
GCCAAATCTC CACCGACAAA CAAGATCGGA AGCAAATCCA GCAACGCCAG CTGGCCTCCA 
GAGTTCCAAC CAGGAGTGCC ATGGAAAGGT ATACAAAACA TTGACCCTGA ATCTGACCCC 
TACGTGACCC CTGGAAGTGT GCTGGGGGGG ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGACAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAATTCCT TCACCAACGT TCATAGCACT 
TCAGCAAAAT TCAGTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAATCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACAGGGCTG 
CCTCGCCCGC CTCCTGGCCT GACCAACCCC AAACCATCAT CTCCATGGAA CAGCACAGCA 
CCCCGATCGG TCAGGGGCTG GGGGGCACAG GACTCAAGGC TTGCCTCTGG TGAGAAGGAT 
CATCTGATAA CGCCATTGAT CAGACCTGAG GAGTGTGCAT TTTACAGCAC TAATGGTTTG 
GAGGAGAGAA AGTTGCAGTT GCCTGCATCT ACTTGGAGTG ATGGTAGCTC AGTTCGTCCT 
AGCTACTGGC TGGTTCTTCA CAATCTCACT CCACAGATCG ATGGGTCAAC CTTGAGGACG 
ATCTGCATGC AGCATGGTCC ACTGCTGACA TTCCATCTGA ACCTGACCCA GGGCACCGCT 
CTCATCCGAT ACAACACCAA ACAGGAGGCG GCCAAGGCCC AGACTGCACT GCACATGTGT 
GTGTTGGGAA ACACTACCAT CCTTGCTGAG TTTGCCACAG ATGAAGAGGT TAGTCGCTTT 
TTGACACAAG CTCAGCCCCC TACACCTGCA GCAACCCCAA GTGCACCCAC GGCAGGCTGG 
CAATCCCTGG AGACAGGACA GAACCAGACA GATCCAGTTG GACCTGCTTT GAATCTCTTT 
GGTGGGTCAA CAGGGCTTGG GCAGTGGAGC AGTGGTGGCG GCGGCAGCAG TGGGGGTGAT 
CTCACTGGCG CTTCGTTGTG GGGGCCCCCA AACTATTCTT CAAGCTTGTG GGGGGTCCCA 
AGCGTAGAGG ATCCACACAG AATGGGCAGC CCTGCTCCCT TACTACCTGG AGACCTTCTG 
GGAGGAGGGT CGGATTCAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025010985.1
CDS	148..5751
Translation	MREKYWRKAVPREKEQEREEQLMEDKKRKKEDKKKKESAQKVTDQKTKVPEVTKPSLSQPVAASPIGNSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPPTAPGANPNNAQPVTGALLQSDSGTATDSTIGGAAASNYANSTWGPGASSSSGTNANPTHIWDKVIVDGSDMEEWPCIASKDAESSSENTTDNNSASNPGLEKNTLPGSTTSNKGKGSQCQSGSAGNECNLGAWKSDPKAKSVQSSNPATESNSGLSNWRNLTGQDRIGSGSGFSNFNPNSNPSAWPALVQEGNSRKGTSESDSGSSNAQISTVGQTSREQQSKMENAGVNFVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFEMGLGNPSRSTDTPSQSTGERKTGSVGSWGTARGSSGTDTVSGQSNSGNHGNNGKDREDSWKGASVQKPNGSRSDSWDNNNRSTGGGSWNFGPQHANESKWGEGNKLTSGVSQGEWKQLSGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNSQAPCWGRSSSSAGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEMPRSEGKSDKGTEGWESSATQTKNSGGWGDVPSQSNQIKSGWGELSTPTEWKDPKNTGGWNDYKNPNSSNWGGGRQEEKSSSSWNDSSSKDQGWGGRQPNQGWSSGKNGWGEEVDQAKNSNWESAGNKPASGWGEGGQNEIGTWGNGANTGSASKGGWDDCKRNSTWNETGRQPNSWNKQQQQQQQQQQETSGSWGPPPQTPSNGRPPNPNWNSGPQPAAPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPSSYNYKNVNLWDKNSQGGQAPREQSLPTPMTSKSQASVWSKSTPPAPDNGTSAWGEPNETSPGWGEVDDTGASTTGWGNAPSNAPNAMKSSSKSMQDGWGESDGPVTGTRHSSWEEEEEGGVWNTAGSQGSSSSHNSTSWGQGGKKTQMKCSLKGGNSDSWMNPISKQFSNMGLLSQTEDIPGNKMDLSVGGLPDKKFEVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLSNVGLGAIGSGLSPQNYAARQGGNHGLFGNSTAQSRGMHTPVQPLNPSPNIRAQVPPQFLSPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQQLLQSQRKLSQAVRQQQEQQIARMVSALQQQQQRQPGMKHSPSHPVGPKPHIDSMVPNPLNVGLSDLQTKGQIPGYGSGFGSSSMDYGMVGGKEAGTESRFKQWTMMEGLPSVASQDANMHKNGAIVPPGKARGGSPYNQFDIIQGDPLGGHAGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFSDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTGLPRPPPGLTNPKPSSPWNSTAPRSVRGWGAQDSRLASGEKDHLITPLIRPEECAFYSTNGLEERKLQLPASTWSDGSSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYNTKQEAAKAQTALHMCVLGNTTILAEFATDEEVSRFLTQAQPPTPAATPSAPTAGWQSLETGQNQTDPVGPALNLFGGSTGLGQWSSGGGGSSGGDLTGASLWGPPNYSSSLWGVPSVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_025155217.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025155217.1

ORF Insert Sequence:

ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGAGAGA AGGAGCAAGA AAGGGAAGAA 
CAGTTAATGG AAGACAAGAA AAGGAAGAAG GAGGATAAGA AAAAGAAGGA ATCTGCTCAG 
AAGGTCACAG ATCAAAAAAC CAAAGTGCCC GAAGTGACGA AACCAAGTTT AAGCCAACCA 
GTGGCTGCCA GCCCAATTGG CAACTCTCCA TCGCCACCAG TCAATGGTGG CAACAATGCC 
AAAAGGGTGG CAGTGCCGAA CGGACAACCG CCAAGCGCCG CCCGCTACAT GCCTCGGGAG 
GTGCCGCCGC GATTCCGTTG CCAGCAGGAC CACAAAGTGT TACTGAAACG TGGGCAGCCC 
CCTCCACCAT CCTGTATGCT CCTTGGGGGT GGAGCAGGGC CTCCTCCCCC TACAGCACCA 
GGAGCAAACC CAAACAACGC ACAACCAGTG ACAGGAGCAC TGCTGCAGAG CGACAGTGGG 
ACTGCAACAG ATTCAACAAT TGGAGGTGCT GCTGCTTCAA ATTATGCAAA TTCCACTTGG 
GGTCCTGGAG CCTCCTCCAG CAGCGGCACC AACGCCAACC CAACTCACAT CTGGGACAAG 
GTGATTGTAG ACGGGTCTGA CATGGAAGAG TGGCCTTGTA TCGCCAGCAA AGATGCTGAG 
TCTTCTTCCG AAAACACCAC CGATAACAAC AGTGCCTCGA ACCCTGGCTT GGAGAAGAAC 
ACTCTGCCAG GAAGCACCAC TAGTAACAAA GGAAAAGGAA GCCAGTGCCA GTCTGGAAGC 
GCTGGAAATG AATGTAATCT TGGGGCCTGG AAATCTGATC CCAAGGCTAA ATCTGTTCAA 
TCTTCCAACC CTGCTACCGA GAGTAACAGT GGACTAAGTA ATTGGAGGAA CTTGACTGGA 
CAAGATAGAA TTGGTTCTGG TTCTGGCTTC AGCAACTTTA ACCCAAATAG CAACCCGTCT 
GCTTGGCCAG CACTGGTCCA AGAGGGCAAT TCTAGGAAAG GGACTTCGGA ATCTGATAGT 
GGTAGCTCCA ATGCACAGAT TAGCACAGTA GGTCAGACCT CTAGGGAACA GCAGTCAAAG 
ATGGAAAATG CGGGTGTTAA CTTTGTCTCT GGCAGAGAAC AGGCTCAAAT TCATAACACT 
GATGGACCAA AAAATGGAAA CACTAACTCC TTGAACTTAA GTTCACCAAA CCCTATGGAG 
AATAAGGGAA TGCCCTTTGA AATGGGCTTG GGGAACCCTT CCAGGAGCAC TGACACCCCT 
TCACAAAGCA CTGGAGAAAG AAAGACTGGG AGTGTTGGAT CTTGGGGTAC AGCTAGGGGG 
TCTTCTGGAA CTGACACAGT CTCTGGACAG AGCAATTCTG GAAACCATGG GAACAATGGA 
AAGGATAGGG AGGACTCCTG GAAAGGAGCT TCTGTTCAAA AACCTAATGG GTCAAGAAGC 
GATTCTTGGG ATAACAATAA CCGGTCTACG GGTGGTGGGT CTTGGAACTT TGGCCCTCAG 
CATGCAAATG AAAGCAAATG GGGTGAAGGG AACAAATTAA CATCTGGGGT CTCTCAGGGA 
GAATGGAAAC AGCTGTCTGG GTCTGATGAA CTGAAGATTG GAGAATGGAG TGGTCCAAAC 
CAACCAAATT CTAGCACTGG AGCATGGGAC AATCAAAAAG GCCACCCTCT TCCTGAAAAC 
CAAGGCAATT CCCAGGCTCC CTGTTGGGGA AGATCTTCCA GCTCTGCAGG AAGTGAGGTT 
GGAGGTCAAA GCACTGGAAG CAACCACAAA GCAGGAAGTA GTGACAGTCA CAATTCTGGA 
CGCCGATCAT ACAGGCCTAC GCATCCTGAT TGCCAGGCAG TCTTGCAGAC TTTGCTGAGC 
AGGACTGATT TGGACCCCCG AGTGCTTTCA AACACTGGCT GGGGCCAAAC TCAAATTAAG 
CAAGATACCG TATGGGATAT TGAAGAGATG CCACGGTCCG AGGGGAAGTC TGATAAAGGA 
ACTGAGGGGT GGGAGAGCTC TGCCACACAG ACAAAGAACT CAGGGGGCTG GGGCGATGTA 
CCCAGCCAAA GCAATCAAAT CAAGTCTGGA TGGGGTGAGC TCTCAACCCC AACAGAATGG 
AAGGACCCCA AAAATACTGG AGGATGGAAT GACTATAAGA ACCCCAATTC GTCTAATTGG 
GGAGGGGGAA GACAAGAAGA GAAATCGTCA TCTTCTTGGA ATGACAGCTC TAGTAAGGAT 
CAGGGGTGGG GTGGACGGCA GCCTAATCAA GGATGGTCTT CTGGAAAGAA TGGTTGGGGA 
GAGGAAGTTG ACCAAGCAAA AAACAGCAAC TGGGAAAGTG CGGGAAATAA GCCAGCATCT 
GGTTGGGGTG AAGGTGGGCA AAATGAGATT GGAACTTGGG GTAACGGTGC AAATACAGGC 
TCTGCTTCAA AGGGTGGTTG GGATGATTGT AAAAGAAATT CAACGTGGAA CGAGACGGGT 
CGACAGCCCA ACTCCTGGAA CAAGCAGCAG CAGCAGCAAC AGCAGCAACA GCAAGAGACT 
TCTGGTTCCT GGGGTCCACC GCCACAAACT CCAAGTAATG GGCGACCTCC AAACCCAAAC 
TGGAACAGCG GACCACAACC AGCTGCCCCC AAAGACGAGG AGCCTAGTGG CTGGGAAGAA 
CCTTCTCCAC AGTCAATCAG TCGAAAAATG GATATTGATG ATGGCACTTC AGCGTGGGGA 
GATCCTAGCA GTTATAACTA CAAGAATGTG AACCTGTGGG ACAAGAACTC ACAAGGGGGA 
CAGGCCCCAC GGGAACAGAG CCTGCCTACT CCAATGACTA GCAAATCACA AGCATCAGTC 
TGGAGCAAAA GCACTCCACC TGCTCCAGAT AATGGTACGT CCGCCTGGGG TGAGCCAAAT 
GAAACCAGTC CCGGGTGGGG TGAAGTAGAT GATACAGGAG CATCGACAAC AGGCTGGGGG 
AATGCACCTT CCAACGCCCC GAATGCCATG AAATCTAGTT CTAAATCTAT GCAAGATGGC 
TGGGGAGAGA GTGATGGGCC AGTGACAGGA ACACGTCATT CCAGCTGGGA AGAGGAGGAG 
GAGGGAGGAG TCTGGAACAC AGCAGGCTCT CAGGGAAGCA GTTCCTCCCA CAACTCAACA 
AGCTGGGGGC AAGGAGGAAA GAAAACGCAG ATGAAGTGCT CGTTAAAAGG AGGAAATAGT 
GATTCATGGA TGAACCCTAT ATCCAAGCAG TTTTCAAATA TGGGCTTGCT AAGTCAAACT 
GAGGACATTC CAGGCAATAA GATGGACTTG TCTGTAGGTG GTCTCCCAGA TAAGAAGTTT 
GAGGTAGATA AACGAGCCAT GAATCTCGGG GATTTTAATG ACATAATGAG AAAGGATCGA 
TCTGGGTTCC GTCCACCTAA TTCCAAAGAC ATGGGAACCA CAGATAGTGG GCCTTATTTT 
GAGAAGCTGA CTTTGCCTTT CTCCAATCAA GATGGGTGCC TTGGGGATGA GGCTCCCTGC 
TCTCCCTTCT CCCCTTCTCC CAGCTACAAG CTGTCTCCCT CTGGTTCCAC ACTATCCAAC 
GTCGGCCTTG GAGCAATCGG CTCAGGGCTC AGTCCCCAAA ACTACGCTGC TAGACAAGGT 
GGCAATCATG GTTTGTTTGG AAACAGCACA GCACAATCGA GGGGCATGCA CACACCAGTG 
CAGCCACTAA ATCCTTCCCC CAATATCCGG GCGCAAGTGC CTCCCCAATT TCTTTCTCCC 
CAGGTTTCGG CCTCCATGCT CAAACAGTTC CCCAACAGTG GCTTGAATCC AGGTCTTTTC 
AATGTGGGGC CCCAGCTTTC TCCACAACAG ATTGCCATGC TGAGCCAGCT TCCACAGATT 
CCCCAGTTTC AATTAGCATG TCAGCTCCTC CTTCAGCAGC AGCAGCAGCA GCAGCAGCTG 
TTACAGAGCC AGAGGAAGTT ATCTCAAGCT GTGCGACAGC AGCAGGAGCA ACAGATTGCT 
CGTATGGTGA GTGCCCTCCA GCAGCAGCAG CAGAGGCAGC CTGGCATGAA GCATTCACCA 
TCCCACCCTG TTGGGCCTAA GCCACATATA GACAGCATGG TACCCAATCC TTTGAATGTG 
GGTCTTTCAG ATTTACAGAC CAAAGGACAA ATACCTGGAT ACGGTTCAGG CTTTGGCTCA 
AGCAGCATGG ATTATGGCAT GGTGGGAGGG AAAGAAGCTG GAACAGAATC CCGCTTTAAA 
CAGTGGACTA TGATGGAAGG GCTGCCCTCT GTGGCTTCAC AAGATGCCAA TATGCACAAA 
AATGGTGCAA TAGTGCCCCC TGGAAAGGCT CGCGGAGGAT CGCCCTACAA CCAGTTCGAC 
ATAATTCAGG GCGACCCACT AGGTGGCCAT GCAGGCCCTG CTGGTGATAG TTGGTTACCT 
GCCAAATCTC CACCGACAAA CAAGATCGGA AGCAAATCCA GCAACGCCAG CTGGCCTCCA 
GAGTTCCAAC CAGGAGTGCC ATGGAAAGGT ATACAAAACA TTGACCCTGA ATCTGACCCC 
TACGTGACCC CTGGAAGTGT GCTGGGGGGG ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGACAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAATTCCT TCACCAACGT TCATAGCACT 
TCAGCAAAAT TCAGTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAATCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACAGGGCTG 
CCTCGCCCGC CTCCTGGCCT GACCAACCCC AAACCATCAT CTCCATGGAA CAGCACAGCA 
CCCCGATCGG TCAGGGGCTG GGGGGCACAG GACTCAAGGC TTGCCTCTGG TGAGAAGGAT 
CATCTGATAA CGCCATTGAT CAGACCTGAG GAGTGTGCAT TTTACAGCAC TAATGGTTTG 
GAGGAGAGAA AGTTGCAGTT GCCTGCATCT ACTTGGAGTG ATGGTAGCTC AGTTCGTCCT 
AGCTACTGGC TGGTTCTTCA CAATCTCACT CCACAGATCG ATGGGTCAAC CTTGAGGACG 
ATCTGCATGC AGCATGGTCC ACTGCTGACA TTCCATCTGA ACCTGACCCA GGGCACCGCT 
CTCATCCGAT ACAACACCAA ACAGGAGGCG GCCAAGGCCC AGACTGCACT GCACATGTGT 
GTGTTGGGAA ACACTACCAT CCTTGCTGAG TTTGCCACAG ATGAAGAGGT TAGTCGCTTT 
TTGACACAAG CTCAGCCCCC TACACCTGCA GCAACCCCAA GTGCACCCAC GGCAGGCTGG 
CAATCCCTGG AGACAGGACA GAACCAGACA GATCCAGTTG GACCTGCTTT GAATCTCTTT 
GGTGGGTCAA CAGGGCTTGG GCAGTGGAGC AGTGGTGGCG GCGGCAGCAG TGGGGGTGAT 
CTCACTGGCG CTTCGTTGTG GGGGCCCCCA AACTATTCTT CAAGCTTGTG GGGGGTCCCA 
AGCGTAGAGG ATCCACACAG AATGGGCAGC CCTGCTCCCT TACTACCTGG AGACCTTCTG 
GGAGGAGGGT CGGATTCAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_004937723.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004937780.1 Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X2, mRNA.

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CloneID	OGa01768
Clone ID Related Accession (Same CDS sequence)	XM_025155217.1 , XM_004937723.2 , XM_004937723.3
Accession Version	XM_004937723.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	trinucleotide repeat-containing gene 6B protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455714.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004937723.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGAGAGA AGGAGCAAGA AAGGGAAGAA 
CAGTTAATGG AAGACAAGAA AAGGAAGAAG GAGGATAAGA AAAAGAAGGA ATCTGCTCAG 
AAGGTCACAG ATCAAAAAAC CAAAGTGCCC GAAGTGACGA AACCAAGTTT AAGCCAACCA 
GTGGCTGCCA GCCCAATTGG CAACTCTCCA TCGCCACCAG TCAATGGTGG CAACAATGCC 
AAAAGGGTGG CAGTGCCGAA CGGACAACCG CCAAGCGCCG CCCGCTACAT GCCTCGGGAG 
GTGCCGCCGC GATTCCGTTG CCAGCAGGAC CACAAAGTGT TACTGAAACG TGGGCAGCCC 
CCTCCACCAT CCTGTATGCT CCTTGGGGGT GGAGCAGGGC CTCCTCCCCC TACAGCACCA 
GGAGCAAACC CAAACAACGC ACAACCAGTG ACAGGAGCAC TGCTGCAGAG CGACAGTGGG 
ACTGCAACAG ATTCAACAAT TGGAGGTGCT GCTGCTTCAA ATTATGCAAA TTCCACTTGG 
GGTCCTGGAG CCTCCTCCAG CAGCGGCACC AACGCCAACC CAACTCACAT CTGGGACAAG 
GTGATTGTAG ACGGGTCTGA CATGGAAGAG TGGCCTTGTA TCGCCAGCAA AGATGCTGAG 
TCTTCTTCCG AAAACACCAC CGATAACAAC AGTGCCTCGA ACCCTGGCTT GGAGAAGAAC 
ACTCTGCCAG GAAGCACCAC TAGTAACAAA GGAAAAGGAA GCCAGTGCCA GTCTGGAAGC 
GCTGGAAATG AATGTAATCT TGGGGCCTGG AAATCTGATC CCAAGGCTAA ATCTGTTCAA 
TCTTCCAACC CTGCTACCGA GAGTAACAGT GGACTAAGTA ATTGGAGGAA CTTGACTGGA 
CAAGATAGAA TTGGTTCTGG TTCTGGCTTC AGCAACTTTA ACCCAAATAG CAACCCGTCT 
GCTTGGCCAG CACTGGTCCA AGAGGGCAAT TCTAGGAAAG GGACTTCGGA ATCTGATAGT 
GGTAGCTCCA ATGCACAGAT TAGCACAGTA GGTCAGACCT CTAGGGAACA GCAGTCAAAG 
ATGGAAAATG CGGGTGTTAA CTTTGTCTCT GGCAGAGAAC AGGCTCAAAT TCATAACACT 
GATGGACCAA AAAATGGAAA CACTAACTCC TTGAACTTAA GTTCACCAAA CCCTATGGAG 
AATAAGGGAA TGCCCTTTGA AATGGGCTTG GGGAACCCTT CCAGGAGCAC TGACACCCCT 
TCACAAAGCA CTGGAGAAAG AAAGACTGGG AGTGTTGGAT CTTGGGGTAC AGCTAGGGGG 
TCTTCTGGAA CTGACACAGT CTCTGGACAG AGCAATTCTG GAAACCATGG GAACAATGGA 
AAGGATAGGG AGGACTCCTG GAAAGGAGCT TCTGTTCAAA AACCTAATGG GTCAAGAAGC 
GATTCTTGGG ATAACAATAA CCGGTCTACG GGTGGTGGGT CTTGGAACTT TGGCCCTCAG 
CATGCAAATG AAAGCAAATG GGGTGAAGGG AACAAATTAA CATCTGGGGT CTCTCAGGGA 
GAATGGAAAC AGCTGTCTGG GTCTGATGAA CTGAAGATTG GAGAATGGAG TGGTCCAAAC 
CAACCAAATT CTAGCACTGG AGCATGGGAC AATCAAAAAG GCCACCCTCT TCCTGAAAAC 
CAAGGCAATT CCCAGGCTCC CTGTTGGGGA AGATCTTCCA GCTCTGCAGG AAGTGAGGTT 
GGAGGTCAAA GCACTGGAAG CAACCACAAA GCAGGAAGTA GTGACAGTCA CAATTCTGGA 
CGCCGATCAT ACAGGCCTAC GCATCCTGAT TGCCAGGCAG TCTTGCAGAC TTTGCTGAGC 
AGGACTGATT TGGACCCCCG AGTGCTTTCA AACACTGGCT GGGGCCAAAC TCAAATTAAG 
CAAGATACCG TATGGGATAT TGAAGAGATG CCACGGTCCG AGGGGAAGTC TGATAAAGGA 
ACTGAGGGGT GGGAGAGCTC TGCCACACAG ACAAAGAACT CAGGGGGCTG GGGCGATGTA 
CCCAGCCAAA GCAATCAAAT CAAGTCTGGA TGGGGTGAGC TCTCAACCCC AACAGAATGG 
AAGGACCCCA AAAATACTGG AGGATGGAAT GACTATAAGA ACCCCAATTC GTCTAATTGG 
GGAGGGGGAA GACAAGAAGA GAAATCGTCA TCTTCTTGGA ATGACAGCTC TAGTAAGGAT 
CAGGGGTGGG GTGGACGGCA GCCTAATCAA GGATGGTCTT CTGGAAAGAA TGGTTGGGGA 
GAGGAAGTTG ACCAAGCAAA AAACAGCAAC TGGGAAAGTG CGGGAAATAA GCCAGCATCT 
GGTTGGGGTG AAGGTGGGCA AAATGAGATT GGAACTTGGG GTAACGGTGC AAATACAGGC 
TCTGCTTCAA AGGGTGGTTG GGATGATTGT AAAAGAAATT CAACGTGGAA CGAGACGGGT 
CGACAGCCCA ACTCCTGGAA CAAGCAGCAG CAGCAGCAAC AGCAGCAACA GCAAGAGACT 
TCTGGTTCCT GGGGTCCACC GCCACAAACT CCAAGTAATG GGCGACCTCC AAACCCAAAC 
TGGAACAGCG GACCACAACC AGCTGCCCCC AAAGACGAGG AGCCTAGTGG CTGGGAAGAA 
CCTTCTCCAC AGTCAATCAG TCGAAAAATG GATATTGATG ATGGCACTTC AGCGTGGGGA 
GATCCTAGCA GTTATAACTA CAAGAATGTG AACCTGTGGG ACAAGAACTC ACAAGGGGGA 
CAGGCCCCAC GGGAACAGAG CCTGCCTACT CCAATGACTA GCAAATCACA AGCATCAGTC 
TGGAGCAAAA GCACTCCACC TGCTCCAGAT AATGGTACGT CCGCCTGGGG TGAGCCAAAT 
GAAACCAGTC CCGGGTGGGG TGAAGTAGAT GATACAGGAG CATCGACAAC AGGCTGGGGG 
AATGCACCTT CCAACGCCCC GAATGCCATG AAATCTAGTT CTAAATCTAT GCAAGATGGC 
TGGGGAGAGA GTGATGGGCC AGTGACAGGA ACACGTCATT CCAGCTGGGA AGAGGAGGAG 
GAGGGAGGAG TCTGGAACAC AGCAGGCTCT CAGGGAAGCA GTTCCTCCCA CAACTCAACA 
AGCTGGGGGC AAGGAGGAAA GAAAACGCAG ATGAAGTGCT CGTTAAAAGG AGGAAATAGT 
GATTCATGGA TGAACCCTAT ATCCAAGCAG TTTTCAAATA TGGGCTTGCT AAGTCAAACT 
GAGGACATTC CAGGCAATAA GATGGACTTG TCTGTAGGTG GTCTCCCAGA TAAGAAGTTT 
GAGGTAGATA AACGAGCCAT GAATCTCGGG GATTTTAATG ACATAATGAG AAAGGATCGA 
TCTGGGTTCC GTCCACCTAA TTCCAAAGAC ATGGGAACCA CAGATAGTGG GCCTTATTTT 
GAGAAGCTGA CTTTGCCTTT CTCCAATCAA GATGGGTGCC TTGGGGATGA GGCTCCCTGC 
TCTCCCTTCT CCCCTTCTCC CAGCTACAAG CTGTCTCCCT CTGGTTCCAC ACTATCCAAC 
GTCGGCCTTG GAGCAATCGG CTCAGGGCTC AGTCCCCAAA ACTACGCTGC TAGACAAGGT 
GGCAATCATG GTTTGTTTGG AAACAGCACA GCACAATCGA GGGGCATGCA CACACCAGTG 
CAGCCACTAA ATCCTTCCCC CAATATCCGG GCGCAAGTGC CTCCCCAATT TCTTTCTCCC 
CAGGTTTCGG CCTCCATGCT CAAACAGTTC CCCAACAGTG GCTTGAATCC AGGTCTTTTC 
AATGTGGGGC CCCAGCTTTC TCCACAACAG ATTGCCATGC TGAGCCAGCT TCCACAGATT 
CCCCAGTTTC AATTAGCATG TCAGCTCCTC CTTCAGCAGC AGCAGCAGCA GCAGCAGCTG 
TTACAGAGCC AGAGGAAGTT ATCTCAAGCT GTGCGACAGC AGCAGGAGCA ACAGATTGCT 
CGTATGGTGA GTGCCCTCCA GCAGCAGCAG CAGAGGCAGC CTGGCATGAA GCATTCACCA 
TCCCACCCTG TTGGGCCTAA GCCACATATA GACAGCATGG TACCCAATCC TTTGAATGTG 
GGTCTTTCAG ATTTACAGAC CAAAGGACAA ATACCTGGAT ACGGTTCAGG CTTTGGCTCA 
AGCAGCATGG ATTATGGCAT GGTGGGAGGG AAAGAAGCTG GAACAGAATC CCGCTTTAAA 
CAGTGGACTA TGATGGAAGG GCTGCCCTCT GTGGCTTCAC AAGATGCCAA TATGCACAAA 
AATGGTGCAA TAGTGCCCCC TGGAAAGGCT CGCGGAGGAT CGCCCTACAA CCAGTTCGAC 
ATAATTCAGG GCGACCCACT AGGTGGCCAT GCAGGCCCTG CTGGTGATAG TTGGTTACCT 
GCCAAATCTC CACCGACAAA CAAGATCGGA AGCAAATCCA GCAACGCCAG CTGGCCTCCA 
GAGTTCCAAC CAGGAGTGCC ATGGAAAGGT ATACAAAACA TTGACCCTGA ATCTGACCCC 
TACGTGACCC CTGGAAGTGT GCTGGGGGGG ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGACAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAATTCCT TCACCAACGT TCATAGCACT 
TCAGCAAAAT TCAGTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAATCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACAGGGCTG 
CCTCGCCCGC CTCCTGGCCT GACCAACCCC AAACCATCAT CTCCATGGAA CAGCACAGCA 
CCCCGATCGG TCAGGGGCTG GGGGGCACAG GACTCAAGGC TTGCCTCTGG TGAGAAGGAT 
CATCTGATAA CGCCATTGAT CAGACCTGAG GAGTGTGCAT TTTACAGCAC TAATGGTTTG 
GAGGAGAGAA AGTTGCAGTT GCCTGCATCT ACTTGGAGTG ATGGTAGCTC AGTTCGTCCT 
AGCTACTGGC TGGTTCTTCA CAATCTCACT CCACAGATCG ATGGGTCAAC CTTGAGGACG 
ATCTGCATGC AGCATGGTCC ACTGCTGACA TTCCATCTGA ACCTGACCCA GGGCACCGCT 
CTCATCCGAT ACAACACCAA ACAGGAGGCG GCCAAGGCCC AGACTGCACT GCACATGTGT 
GTGTTGGGAA ACACTACCAT CCTTGCTGAG TTTGCCACAG ATGAAGAGGT TAGTCGCTTT 
TTGACACAAG CTCAGCCCCC TACACCTGCA GCAACCCCAA GTGCACCCAC GGCAGGCTGG 
CAATCCCTGG AGACAGGACA GAACCAGACA GATCCAGTTG GACCTGCTTT GAATCTCTTT 
GGTGGGTCAA CAGGGCTTGG GCAGTGGAGC AGTGGTGGCG GCGGCAGCAG TGGGGGTGAT 
CTCACTGGCG CTTCGTTGTG GGGGCCCCCA AACTATTCTT CAAGCTTGTG GGGGGTCCCA 
AGCGTAGAGG ATCCACACAG AATGGGCAGC CCTGCTCCCT TACTACCTGG AGACCTTCTG 
GGAGGAGGGT CGGATTCAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004937780.1
CDS	550..6153
Translation	MREKYWRKAVPREKEQEREEQLMEDKKRKKEDKKKKESAQKVTDQKTKVPEVTKPSLSQPVAASPIGNSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPPTAPGANPNNAQPVTGALLQSDSGTATDSTIGGAAASNYANSTWGPGASSSSGTNANPTHIWDKVIVDGSDMEEWPCIASKDAESSSENTTDNNSASNPGLEKNTLPGSTTSNKGKGSQCQSGSAGNECNLGAWKSDPKAKSVQSSNPATESNSGLSNWRNLTGQDRIGSGSGFSNFNPNSNPSAWPALVQEGNSRKGTSESDSGSSNAQISTVGQTSREQQSKMENAGVNFVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFEMGLGNPSRSTDTPSQSTGERKTGSVGSWGTARGSSGTDTVSGQSNSGNHGNNGKDREDSWKGASVQKPNGSRSDSWDNNNRSTGGGSWNFGPQHANESKWGEGNKLTSGVSQGEWKQLSGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNSQAPCWGRSSSSAGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEMPRSEGKSDKGTEGWESSATQTKNSGGWGDVPSQSNQIKSGWGELSTPTEWKDPKNTGGWNDYKNPNSSNWGGGRQEEKSSSSWNDSSSKDQGWGGRQPNQGWSSGKNGWGEEVDQAKNSNWESAGNKPASGWGEGGQNEIGTWGNGANTGSASKGGWDDCKRNSTWNETGRQPNSWNKQQQQQQQQQQETSGSWGPPPQTPSNGRPPNPNWNSGPQPAAPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPSSYNYKNVNLWDKNSQGGQAPREQSLPTPMTSKSQASVWSKSTPPAPDNGTSAWGEPNETSPGWGEVDDTGASTTGWGNAPSNAPNAMKSSSKSMQDGWGESDGPVTGTRHSSWEEEEEGGVWNTAGSQGSSSSHNSTSWGQGGKKTQMKCSLKGGNSDSWMNPISKQFSNMGLLSQTEDIPGNKMDLSVGGLPDKKFEVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLSNVGLGAIGSGLSPQNYAARQGGNHGLFGNSTAQSRGMHTPVQPLNPSPNIRAQVPPQFLSPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQQLLQSQRKLSQAVRQQQEQQIARMVSALQQQQQRQPGMKHSPSHPVGPKPHIDSMVPNPLNVGLSDLQTKGQIPGYGSGFGSSSMDYGMVGGKEAGTESRFKQWTMMEGLPSVASQDANMHKNGAIVPPGKARGGSPYNQFDIIQGDPLGGHAGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFSDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTGLPRPPPGLTNPKPSSPWNSTAPRSVRGWGAQDSRLASGEKDHLITPLIRPEECAFYSTNGLEERKLQLPASTWSDGSSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYNTKQEAAKAQTALHMCVLGNTTILAEFATDEEVSRFLTQAQPPTPAATPSAPTAGWQSLETGQNQTDPVGPALNLFGGSTGLGQWSSGGGGSSGGDLTGASLWGPPNYSSSLWGVPSVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_004937723.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004937723.2

ORF Insert Sequence:

ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGAGAGA AGGAGCAAGA AAGGGAAGAA 
CAGTTAATGG AAGACAAGAA AAGGAAGAAG GAGGATAAGA AAAAGAAGGA ATCTGCTCAG 
AAGGTCACAG ATCAAAAAAC CAAAGTGCCC GAAGTGACGA AACCAAGTTT AAGCCAACCA 
GTGGCTGCCA GCCCAATTGG CAACTCTCCA TCGCCACCAG TCAATGGTGG CAACAATGCC 
AAAAGGGTGG CAGTGCCGAA CGGACAACCG CCAAGCGCCG CCCGCTACAT GCCTCGGGAG 
GTGCCGCCGC GATTCCGTTG CCAGCAGGAC CACAAAGTGT TACTGAAACG TGGGCAGCCC 
CCTCCACCAT CCTGTATGCT CCTTGGGGGT GGAGCAGGGC CTCCTCCCCC TACAGCACCA 
GGAGCAAACC CAAACAACGC ACAACCAGTG ACAGGAGCAC TGCTGCAGAG CGACAGTGGG 
ACTGCAACAG ATTCAACAAT TGGAGGTGCT GCTGCTTCAA ATTATGCAAA TTCCACTTGG 
GGTCCTGGAG CCTCCTCCAG CAGCGGCACC AACGCCAACC CAACTCACAT CTGGGACAAG 
GTGATTGTAG ACGGGTCTGA CATGGAAGAG TGGCCTTGTA TCGCCAGCAA AGATGCTGAG 
TCTTCTTCCG AAAACACCAC CGATAACAAC AGTGCCTCGA ACCCTGGCTT GGAGAAGAAC 
ACTCTGCCAG GAAGCACCAC TAGTAACAAA GGAAAAGGAA GCCAGTGCCA GTCTGGAAGC 
GCTGGAAATG AATGTAATCT TGGGGCCTGG AAATCTGATC CCAAGGCTAA ATCTGTTCAA 
TCTTCCAACC CTGCTACCGA GAGTAACAGT GGACTAAGTA ATTGGAGGAA CTTGACTGGA 
CAAGATAGAA TTGGTTCTGG TTCTGGCTTC AGCAACTTTA ACCCAAATAG CAACCCGTCT 
GCTTGGCCAG CACTGGTCCA AGAGGGCAAT TCTAGGAAAG GGACTTCGGA ATCTGATAGT 
GGTAGCTCCA ATGCACAGAT TAGCACAGTA GGTCAGACCT CTAGGGAACA GCAGTCAAAG 
ATGGAAAATG CGGGTGTTAA CTTTGTCTCT GGCAGAGAAC AGGCTCAAAT TCATAACACT 
GATGGACCAA AAAATGGAAA CACTAACTCC TTGAACTTAA GTTCACCAAA CCCTATGGAG 
AATAAGGGAA TGCCCTTTGA AATGGGCTTG GGGAACCCTT CCAGGAGCAC TGACACCCCT 
TCACAAAGCA CTGGAGAAAG AAAGACTGGG AGTGTTGGAT CTTGGGGTAC AGCTAGGGGG 
TCTTCTGGAA CTGACACAGT CTCTGGACAG AGCAATTCTG GAAACCATGG GAACAATGGA 
AAGGATAGGG AGGACTCCTG GAAAGGAGCT TCTGTTCAAA AACCTAATGG GTCAAGAAGC 
GATTCTTGGG ATAACAATAA CCGGTCTACG GGTGGTGGGT CTTGGAACTT TGGCCCTCAG 
CATGCAAATG AAAGCAAATG GGGTGAAGGG AACAAATTAA CATCTGGGGT CTCTCAGGGA 
GAATGGAAAC AGCTGTCTGG GTCTGATGAA CTGAAGATTG GAGAATGGAG TGGTCCAAAC 
CAACCAAATT CTAGCACTGG AGCATGGGAC AATCAAAAAG GCCACCCTCT TCCTGAAAAC 
CAAGGCAATT CCCAGGCTCC CTGTTGGGGA AGATCTTCCA GCTCTGCAGG AAGTGAGGTT 
GGAGGTCAAA GCACTGGAAG CAACCACAAA GCAGGAAGTA GTGACAGTCA CAATTCTGGA 
CGCCGATCAT ACAGGCCTAC GCATCCTGAT TGCCAGGCAG TCTTGCAGAC TTTGCTGAGC 
AGGACTGATT TGGACCCCCG AGTGCTTTCA AACACTGGCT GGGGCCAAAC TCAAATTAAG 
CAAGATACCG TATGGGATAT TGAAGAGATG CCACGGTCCG AGGGGAAGTC TGATAAAGGA 
ACTGAGGGGT GGGAGAGCTC TGCCACACAG ACAAAGAACT CAGGGGGCTG GGGCGATGTA 
CCCAGCCAAA GCAATCAAAT CAAGTCTGGA TGGGGTGAGC TCTCAACCCC AACAGAATGG 
AAGGACCCCA AAAATACTGG AGGATGGAAT GACTATAAGA ACCCCAATTC GTCTAATTGG 
GGAGGGGGAA GACAAGAAGA GAAATCGTCA TCTTCTTGGA ATGACAGCTC TAGTAAGGAT 
CAGGGGTGGG GTGGACGGCA GCCTAATCAA GGATGGTCTT CTGGAAAGAA TGGTTGGGGA 
GAGGAAGTTG ACCAAGCAAA AAACAGCAAC TGGGAAAGTG CGGGAAATAA GCCAGCATCT 
GGTTGGGGTG AAGGTGGGCA AAATGAGATT GGAACTTGGG GTAACGGTGC AAATACAGGC 
TCTGCTTCAA AGGGTGGTTG GGATGATTGT AAAAGAAATT CAACGTGGAA CGAGACGGGT 
CGACAGCCCA ACTCCTGGAA CAAGCAGCAG CAGCAGCAAC AGCAGCAACA GCAAGAGACT 
TCTGGTTCCT GGGGTCCACC GCCACAAACT CCAAGTAATG GGCGACCTCC AAACCCAAAC 
TGGAACAGCG GACCACAACC AGCTGCCCCC AAAGACGAGG AGCCTAGTGG CTGGGAAGAA 
CCTTCTCCAC AGTCAATCAG TCGAAAAATG GATATTGATG ATGGCACTTC AGCGTGGGGA 
GATCCTAGCA GTTATAACTA CAAGAATGTG AACCTGTGGG ACAAGAACTC ACAAGGGGGA 
CAGGCCCCAC GGGAACAGAG CCTGCCTACT CCAATGACTA GCAAATCACA AGCATCAGTC 
TGGAGCAAAA GCACTCCACC TGCTCCAGAT AATGGTACGT CCGCCTGGGG TGAGCCAAAT 
GAAACCAGTC CCGGGTGGGG TGAAGTAGAT GATACAGGAG CATCGACAAC AGGCTGGGGG 
AATGCACCTT CCAACGCCCC GAATGCCATG AAATCTAGTT CTAAATCTAT GCAAGATGGC 
TGGGGAGAGA GTGATGGGCC AGTGACAGGA ACACGTCATT CCAGCTGGGA AGAGGAGGAG 
GAGGGAGGAG TCTGGAACAC AGCAGGCTCT CAGGGAAGCA GTTCCTCCCA CAACTCAACA 
AGCTGGGGGC AAGGAGGAAA GAAAACGCAG ATGAAGTGCT CGTTAAAAGG AGGAAATAGT 
GATTCATGGA TGAACCCTAT ATCCAAGCAG TTTTCAAATA TGGGCTTGCT AAGTCAAACT 
GAGGACATTC CAGGCAATAA GATGGACTTG TCTGTAGGTG GTCTCCCAGA TAAGAAGTTT 
GAGGTAGATA AACGAGCCAT GAATCTCGGG GATTTTAATG ACATAATGAG AAAGGATCGA 
TCTGGGTTCC GTCCACCTAA TTCCAAAGAC ATGGGAACCA CAGATAGTGG GCCTTATTTT 
GAGAAGCTGA CTTTGCCTTT CTCCAATCAA GATGGGTGCC TTGGGGATGA GGCTCCCTGC 
TCTCCCTTCT CCCCTTCTCC CAGCTACAAG CTGTCTCCCT CTGGTTCCAC ACTATCCAAC 
GTCGGCCTTG GAGCAATCGG CTCAGGGCTC AGTCCCCAAA ACTACGCTGC TAGACAAGGT 
GGCAATCATG GTTTGTTTGG AAACAGCACA GCACAATCGA GGGGCATGCA CACACCAGTG 
CAGCCACTAA ATCCTTCCCC CAATATCCGG GCGCAAGTGC CTCCCCAATT TCTTTCTCCC 
CAGGTTTCGG CCTCCATGCT CAAACAGTTC CCCAACAGTG GCTTGAATCC AGGTCTTTTC 
AATGTGGGGC CCCAGCTTTC TCCACAACAG ATTGCCATGC TGAGCCAGCT TCCACAGATT 
CCCCAGTTTC AATTAGCATG TCAGCTCCTC CTTCAGCAGC AGCAGCAGCA GCAGCAGCTG 
TTACAGAGCC AGAGGAAGTT ATCTCAAGCT GTGCGACAGC AGCAGGAGCA ACAGATTGCT 
CGTATGGTGA GTGCCCTCCA GCAGCAGCAG CAGAGGCAGC CTGGCATGAA GCATTCACCA 
TCCCACCCTG TTGGGCCTAA GCCACATATA GACAGCATGG TACCCAATCC TTTGAATGTG 
GGTCTTTCAG ATTTACAGAC CAAAGGACAA ATACCTGGAT ACGGTTCAGG CTTTGGCTCA 
AGCAGCATGG ATTATGGCAT GGTGGGAGGG AAAGAAGCTG GAACAGAATC CCGCTTTAAA 
CAGTGGACTA TGATGGAAGG GCTGCCCTCT GTGGCTTCAC AAGATGCCAA TATGCACAAA 
AATGGTGCAA TAGTGCCCCC TGGAAAGGCT CGCGGAGGAT CGCCCTACAA CCAGTTCGAC 
ATAATTCAGG GCGACCCACT AGGTGGCCAT GCAGGCCCTG CTGGTGATAG TTGGTTACCT 
GCCAAATCTC CACCGACAAA CAAGATCGGA AGCAAATCCA GCAACGCCAG CTGGCCTCCA 
GAGTTCCAAC CAGGAGTGCC ATGGAAAGGT ATACAAAACA TTGACCCTGA ATCTGACCCC 
TACGTGACCC CTGGAAGTGT GCTGGGGGGG ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGACAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAATTCCT TCACCAACGT TCATAGCACT 
TCAGCAAAAT TCAGTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAATCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACAGGGCTG 
CCTCGCCCGC CTCCTGGCCT GACCAACCCC AAACCATCAT CTCCATGGAA CAGCACAGCA 
CCCCGATCGG TCAGGGGCTG GGGGGCACAG GACTCAAGGC TTGCCTCTGG TGAGAAGGAT 
CATCTGATAA CGCCATTGAT CAGACCTGAG GAGTGTGCAT TTTACAGCAC TAATGGTTTG 
GAGGAGAGAA AGTTGCAGTT GCCTGCATCT ACTTGGAGTG ATGGTAGCTC AGTTCGTCCT 
AGCTACTGGC TGGTTCTTCA CAATCTCACT CCACAGATCG ATGGGTCAAC CTTGAGGACG 
ATCTGCATGC AGCATGGTCC ACTGCTGACA TTCCATCTGA ACCTGACCCA GGGCACCGCT 
CTCATCCGAT ACAACACCAA ACAGGAGGCG GCCAAGGCCC AGACTGCACT GCACATGTGT 
GTGTTGGGAA ACACTACCAT CCTTGCTGAG TTTGCCACAG ATGAAGAGGT TAGTCGCTTT 
TTGACACAAG CTCAGCCCCC TACACCTGCA GCAACCCCAA GTGCACCCAC GGCAGGCTGG 
CAATCCCTGG AGACAGGACA GAACCAGACA GATCCAGTTG GACCTGCTTT GAATCTCTTT 
GGTGGGTCAA CAGGGCTTGG GCAGTGGAGC AGTGGTGGCG GCGGCAGCAG TGGGGGTGAT 
CTCACTGGCG CTTCGTTGTG GGGGCCCCCA AACTATTCTT CAAGCTTGTG GGGGGTCCCA 
AGCGTAGAGG ATCCACACAG AATGGGCAGC CCTGCTCCCT TACTACCTGG AGACCTTCTG 
GGAGGAGGGT CGGATTCAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_004937723.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004937780.1 Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X2, mRNA.

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CloneID	OGa01768
Clone ID Related Accession (Same CDS sequence)	XM_025155217.1 , XM_004937723.2 , XM_004937723.3
Accession Version	XM_004937723.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	trinucleotide repeat-containing gene 6B protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004937723.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGAGAGA AGGAGCAAGA AAGGGAAGAA 
CAGTTAATGG AAGACAAGAA AAGGAAGAAG GAGGATAAGA AAAAGAAGGA ATCTGCTCAG 
AAGGTCACAG ATCAAAAAAC CAAAGTGCCC GAAGTGACGA AACCAAGTTT AAGCCAACCA 
GTGGCTGCCA GCCCAATTGG CAACTCTCCA TCGCCACCAG TCAATGGTGG CAACAATGCC 
AAAAGGGTGG CAGTGCCGAA CGGACAACCG CCAAGCGCCG CCCGCTACAT GCCTCGGGAG 
GTGCCGCCGC GATTCCGTTG CCAGCAGGAC CACAAAGTGT TACTGAAACG TGGGCAGCCC 
CCTCCACCAT CCTGTATGCT CCTTGGGGGT GGAGCAGGGC CTCCTCCCCC TACAGCACCA 
GGAGCAAACC CAAACAACGC ACAACCAGTG ACAGGAGCAC TGCTGCAGAG CGACAGTGGG 
ACTGCAACAG ATTCAACAAT TGGAGGTGCT GCTGCTTCAA ATTATGCAAA TTCCACTTGG 
GGTCCTGGAG CCTCCTCCAG CAGCGGCACC AACGCCAACC CAACTCACAT CTGGGACAAG 
GTGATTGTAG ACGGGTCTGA CATGGAAGAG TGGCCTTGTA TCGCCAGCAA AGATGCTGAG 
TCTTCTTCCG AAAACACCAC CGATAACAAC AGTGCCTCGA ACCCTGGCTT GGAGAAGAAC 
ACTCTGCCAG GAAGCACCAC TAGTAACAAA GGAAAAGGAA GCCAGTGCCA GTCTGGAAGC 
GCTGGAAATG AATGTAATCT TGGGGCCTGG AAATCTGATC CCAAGGCTAA ATCTGTTCAA 
TCTTCCAACC CTGCTACCGA GAGTAACAGT GGACTAAGTA ATTGGAGGAA CTTGACTGGA 
CAAGATAGAA TTGGTTCTGG TTCTGGCTTC AGCAACTTTA ACCCAAATAG CAACCCGTCT 
GCTTGGCCAG CACTGGTCCA AGAGGGCAAT TCTAGGAAAG GGACTTCGGA ATCTGATAGT 
GGTAGCTCCA ATGCACAGAT TAGCACAGTA GGTCAGACCT CTAGGGAACA GCAGTCAAAG 
ATGGAAAATG CGGGTGTTAA CTTTGTCTCT GGCAGAGAAC AGGCTCAAAT TCATAACACT 
GATGGACCAA AAAATGGAAA CACTAACTCC TTGAACTTAA GTTCACCAAA CCCTATGGAG 
AATAAGGGAA TGCCCTTTGA AATGGGCTTG GGGAACCCTT CCAGGAGCAC TGACACCCCT 
TCACAAAGCA CTGGAGAAAG AAAGACTGGG AGTGTTGGAT CTTGGGGTAC AGCTAGGGGG 
TCTTCTGGAA CTGACACAGT CTCTGGACAG AGCAATTCTG GAAACCATGG GAACAATGGA 
AAGGATAGGG AGGACTCCTG GAAAGGAGCT TCTGTTCAAA AACCTAATGG GTCAAGAAGC 
GATTCTTGGG ATAACAATAA CCGGTCTACG GGTGGTGGGT CTTGGAACTT TGGCCCTCAG 
CATGCAAATG AAAGCAAATG GGGTGAAGGG AACAAATTAA CATCTGGGGT CTCTCAGGGA 
GAATGGAAAC AGCTGTCTGG GTCTGATGAA CTGAAGATTG GAGAATGGAG TGGTCCAAAC 
CAACCAAATT CTAGCACTGG AGCATGGGAC AATCAAAAAG GCCACCCTCT TCCTGAAAAC 
CAAGGCAATT CCCAGGCTCC CTGTTGGGGA AGATCTTCCA GCTCTGCAGG AAGTGAGGTT 
GGAGGTCAAA GCACTGGAAG CAACCACAAA GCAGGAAGTA GTGACAGTCA CAATTCTGGA 
CGCCGATCAT ACAGGCCTAC GCATCCTGAT TGCCAGGCAG TCTTGCAGAC TTTGCTGAGC 
AGGACTGATT TGGACCCCCG AGTGCTTTCA AACACTGGCT GGGGCCAAAC TCAAATTAAG 
CAAGATACCG TATGGGATAT TGAAGAGATG CCACGGTCCG AGGGGAAGTC TGATAAAGGA 
ACTGAGGGGT GGGAGAGCTC TGCCACACAG ACAAAGAACT CAGGGGGCTG GGGCGATGTA 
CCCAGCCAAA GCAATCAAAT CAAGTCTGGA TGGGGTGAGC TCTCAACCCC AACAGAATGG 
AAGGACCCCA AAAATACTGG AGGATGGAAT GACTATAAGA ACCCCAATTC GTCTAATTGG 
GGAGGGGGAA GACAAGAAGA GAAATCGTCA TCTTCTTGGA ATGACAGCTC TAGTAAGGAT 
CAGGGGTGGG GTGGACGGCA GCCTAATCAA GGATGGTCTT CTGGAAAGAA TGGTTGGGGA 
GAGGAAGTTG ACCAAGCAAA AAACAGCAAC TGGGAAAGTG CGGGAAATAA GCCAGCATCT 
GGTTGGGGTG AAGGTGGGCA AAATGAGATT GGAACTTGGG GTAACGGTGC AAATACAGGC 
TCTGCTTCAA AGGGTGGTTG GGATGATTGT AAAAGAAATT CAACGTGGAA CGAGACGGGT 
CGACAGCCCA ACTCCTGGAA CAAGCAGCAG CAGCAGCAAC AGCAGCAACA GCAAGAGACT 
TCTGGTTCCT GGGGTCCACC GCCACAAACT CCAAGTAATG GGCGACCTCC AAACCCAAAC 
TGGAACAGCG GACCACAACC AGCTGCCCCC AAAGACGAGG AGCCTAGTGG CTGGGAAGAA 
CCTTCTCCAC AGTCAATCAG TCGAAAAATG GATATTGATG ATGGCACTTC AGCGTGGGGA 
GATCCTAGCA GTTATAACTA CAAGAATGTG AACCTGTGGG ACAAGAACTC ACAAGGGGGA 
CAGGCCCCAC GGGAACAGAG CCTGCCTACT CCAATGACTA GCAAATCACA AGCATCAGTC 
TGGAGCAAAA GCACTCCACC TGCTCCAGAT AATGGTACGT CCGCCTGGGG TGAGCCAAAT 
GAAACCAGTC CCGGGTGGGG TGAAGTAGAT GATACAGGAG CATCGACAAC AGGCTGGGGG 
AATGCACCTT CCAACGCCCC GAATGCCATG AAATCTAGTT CTAAATCTAT GCAAGATGGC 
TGGGGAGAGA GTGATGGGCC AGTGACAGGA ACACGTCATT CCAGCTGGGA AGAGGAGGAG 
GAGGGAGGAG TCTGGAACAC AGCAGGCTCT CAGGGAAGCA GTTCCTCCCA CAACTCAACA 
AGCTGGGGGC AAGGAGGAAA GAAAACGCAG ATGAAGTGCT CGTTAAAAGG AGGAAATAGT 
GATTCATGGA TGAACCCTAT ATCCAAGCAG TTTTCAAATA TGGGCTTGCT AAGTCAAACT 
GAGGACATTC CAGGCAATAA GATGGACTTG TCTGTAGGTG GTCTCCCAGA TAAGAAGTTT 
GAGGTAGATA AACGAGCCAT GAATCTCGGG GATTTTAATG ACATAATGAG AAAGGATCGA 
TCTGGGTTCC GTCCACCTAA TTCCAAAGAC ATGGGAACCA CAGATAGTGG GCCTTATTTT 
GAGAAGCTGA CTTTGCCTTT CTCCAATCAA GATGGGTGCC TTGGGGATGA GGCTCCCTGC 
TCTCCCTTCT CCCCTTCTCC CAGCTACAAG CTGTCTCCCT CTGGTTCCAC ACTATCCAAC 
GTCGGCCTTG GAGCAATCGG CTCAGGGCTC AGTCCCCAAA ACTACGCTGC TAGACAAGGT 
GGCAATCATG GTTTGTTTGG AAACAGCACA GCACAATCGA GGGGCATGCA CACACCAGTG 
CAGCCACTAA ATCCTTCCCC CAATATCCGG GCGCAAGTGC CTCCCCAATT TCTTTCTCCC 
CAGGTTTCGG CCTCCATGCT CAAACAGTTC CCCAACAGTG GCTTGAATCC AGGTCTTTTC 
AATGTGGGGC CCCAGCTTTC TCCACAACAG ATTGCCATGC TGAGCCAGCT TCCACAGATT 
CCCCAGTTTC AATTAGCATG TCAGCTCCTC CTTCAGCAGC AGCAGCAGCA GCAGCAGCTG 
TTACAGAGCC AGAGGAAGTT ATCTCAAGCT GTGCGACAGC AGCAGGAGCA ACAGATTGCT 
CGTATGGTGA GTGCCCTCCA GCAGCAGCAG CAGAGGCAGC CTGGCATGAA GCATTCACCA 
TCCCACCCTG TTGGGCCTAA GCCACATATA GACAGCATGG TACCCAATCC TTTGAATGTG 
GGTCTTTCAG ATTTACAGAC CAAAGGACAA ATACCTGGAT ACGGTTCAGG CTTTGGCTCA 
AGCAGCATGG ATTATGGCAT GGTGGGAGGG AAAGAAGCTG GAACAGAATC CCGCTTTAAA 
CAGTGGACTA TGATGGAAGG GCTGCCCTCT GTGGCTTCAC AAGATGCCAA TATGCACAAA 
AATGGTGCAA TAGTGCCCCC TGGAAAGGCT CGCGGAGGAT CGCCCTACAA CCAGTTCGAC 
ATAATTCAGG GCGACCCACT AGGTGGCCAT GCAGGCCCTG CTGGTGATAG TTGGTTACCT 
GCCAAATCTC CACCGACAAA CAAGATCGGA AGCAAATCCA GCAACGCCAG CTGGCCTCCA 
GAGTTCCAAC CAGGAGTGCC ATGGAAAGGT ATACAAAACA TTGACCCTGA ATCTGACCCC 
TACGTGACCC CTGGAAGTGT GCTGGGGGGG ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGACAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAATTCCT TCACCAACGT TCATAGCACT 
TCAGCAAAAT TCAGTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAATCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACAGGGCTG 
CCTCGCCCGC CTCCTGGCCT GACCAACCCC AAACCATCAT CTCCATGGAA CAGCACAGCA 
CCCCGATCGG TCAGGGGCTG GGGGGCACAG GACTCAAGGC TTGCCTCTGG TGAGAAGGAT 
CATCTGATAA CGCCATTGAT CAGACCTGAG GAGTGTGCAT TTTACAGCAC TAATGGTTTG 
GAGGAGAGAA AGTTGCAGTT GCCTGCATCT ACTTGGAGTG ATGGTAGCTC AGTTCGTCCT 
AGCTACTGGC TGGTTCTTCA CAATCTCACT CCACAGATCG ATGGGTCAAC CTTGAGGACG 
ATCTGCATGC AGCATGGTCC ACTGCTGACA TTCCATCTGA ACCTGACCCA GGGCACCGCT 
CTCATCCGAT ACAACACCAA ACAGGAGGCG GCCAAGGCCC AGACTGCACT GCACATGTGT 
GTGTTGGGAA ACACTACCAT CCTTGCTGAG TTTGCCACAG ATGAAGAGGT TAGTCGCTTT 
TTGACACAAG CTCAGCCCCC TACACCTGCA GCAACCCCAA GTGCACCCAC GGCAGGCTGG 
CAATCCCTGG AGACAGGACA GAACCAGACA GATCCAGTTG GACCTGCTTT GAATCTCTTT 
GGTGGGTCAA CAGGGCTTGG GCAGTGGAGC AGTGGTGGCG GCGGCAGCAG TGGGGGTGAT 
CTCACTGGCG CTTCGTTGTG GGGGCCCCCA AACTATTCTT CAAGCTTGTG GGGGGTCCCA 
AGCGTAGAGG ATCCACACAG AATGGGCAGC CCTGCTCCCT TACTACCTGG AGACCTTCTG 
GGAGGAGGGT CGGATTCAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004937780.1
CDS	561..6164
Translation	MREKYWRKAVPREKEQEREEQLMEDKKRKKEDKKKKESAQKVTDQKTKVPEVTKPSLSQPVAASPIGNSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPPTAPGANPNNAQPVTGALLQSDSGTATDSTIGGAAASNYANSTWGPGASSSSGTNANPTHIWDKVIVDGSDMEEWPCIASKDAESSSENTTDNNSASNPGLEKNTLPGSTTSNKGKGSQCQSGSAGNECNLGAWKSDPKAKSVQSSNPATESNSGLSNWRNLTGQDRIGSGSGFSNFNPNSNPSAWPALVQEGNSRKGTSESDSGSSNAQISTVGQTSREQQSKMENAGVNFVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFEMGLGNPSRSTDTPSQSTGERKTGSVGSWGTARGSSGTDTVSGQSNSGNHGNNGKDREDSWKGASVQKPNGSRSDSWDNNNRSTGGGSWNFGPQHANESKWGEGNKLTSGVSQGEWKQLSGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNSQAPCWGRSSSSAGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEMPRSEGKSDKGTEGWESSATQTKNSGGWGDVPSQSNQIKSGWGELSTPTEWKDPKNTGGWNDYKNPNSSNWGGGRQEEKSSSSWNDSSSKDQGWGGRQPNQGWSSGKNGWGEEVDQAKNSNWESAGNKPASGWGEGGQNEIGTWGNGANTGSASKGGWDDCKRNSTWNETGRQPNSWNKQQQQQQQQQQETSGSWGPPPQTPSNGRPPNPNWNSGPQPAAPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPSSYNYKNVNLWDKNSQGGQAPREQSLPTPMTSKSQASVWSKSTPPAPDNGTSAWGEPNETSPGWGEVDDTGASTTGWGNAPSNAPNAMKSSSKSMQDGWGESDGPVTGTRHSSWEEEEEGGVWNTAGSQGSSSSHNSTSWGQGGKKTQMKCSLKGGNSDSWMNPISKQFSNMGLLSQTEDIPGNKMDLSVGGLPDKKFEVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLSNVGLGAIGSGLSPQNYAARQGGNHGLFGNSTAQSRGMHTPVQPLNPSPNIRAQVPPQFLSPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQQLLQSQRKLSQAVRQQQEQQIARMVSALQQQQQRQPGMKHSPSHPVGPKPHIDSMVPNPLNVGLSDLQTKGQIPGYGSGFGSSSMDYGMVGGKEAGTESRFKQWTMMEGLPSVASQDANMHKNGAIVPPGKARGGSPYNQFDIIQGDPLGGHAGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFSDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTGLPRPPPGLTNPKPSSPWNSTAPRSVRGWGAQDSRLASGEKDHLITPLIRPEECAFYSTNGLEERKLQLPASTWSDGSSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYNTKQEAAKAQTALHMCVLGNTTILAEFATDEEVSRFLTQAQPPTPAATPSAPTAGWQSLETGQNQTDPVGPALNLFGGSTGLGQWSSGGGGSSGGDLTGASLWGPPNYSSSLWGVPSVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_004937723.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004937723.3

ORF Insert Sequence:

ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGAGAGA AGGAGCAAGA AAGGGAAGAA 
CAGTTAATGG AAGACAAGAA AAGGAAGAAG GAGGATAAGA AAAAGAAGGA ATCTGCTCAG 
AAGGTCACAG ATCAAAAAAC CAAAGTGCCC GAAGTGACGA AACCAAGTTT AAGCCAACCA 
GTGGCTGCCA GCCCAATTGG CAACTCTCCA TCGCCACCAG TCAATGGTGG CAACAATGCC 
AAAAGGGTGG CAGTGCCGAA CGGACAACCG CCAAGCGCCG CCCGCTACAT GCCTCGGGAG 
GTGCCGCCGC GATTCCGTTG CCAGCAGGAC CACAAAGTGT TACTGAAACG TGGGCAGCCC 
CCTCCACCAT CCTGTATGCT CCTTGGGGGT GGAGCAGGGC CTCCTCCCCC TACAGCACCA 
GGAGCAAACC CAAACAACGC ACAACCAGTG ACAGGAGCAC TGCTGCAGAG CGACAGTGGG 
ACTGCAACAG ATTCAACAAT TGGAGGTGCT GCTGCTTCAA ATTATGCAAA TTCCACTTGG 
GGTCCTGGAG CCTCCTCCAG CAGCGGCACC AACGCCAACC CAACTCACAT CTGGGACAAG 
GTGATTGTAG ACGGGTCTGA CATGGAAGAG TGGCCTTGTA TCGCCAGCAA AGATGCTGAG 
TCTTCTTCCG AAAACACCAC CGATAACAAC AGTGCCTCGA ACCCTGGCTT GGAGAAGAAC 
ACTCTGCCAG GAAGCACCAC TAGTAACAAA GGAAAAGGAA GCCAGTGCCA GTCTGGAAGC 
GCTGGAAATG AATGTAATCT TGGGGCCTGG AAATCTGATC CCAAGGCTAA ATCTGTTCAA 
TCTTCCAACC CTGCTACCGA GAGTAACAGT GGACTAAGTA ATTGGAGGAA CTTGACTGGA 
CAAGATAGAA TTGGTTCTGG TTCTGGCTTC AGCAACTTTA ACCCAAATAG CAACCCGTCT 
GCTTGGCCAG CACTGGTCCA AGAGGGCAAT TCTAGGAAAG GGACTTCGGA ATCTGATAGT 
GGTAGCTCCA ATGCACAGAT TAGCACAGTA GGTCAGACCT CTAGGGAACA GCAGTCAAAG 
ATGGAAAATG CGGGTGTTAA CTTTGTCTCT GGCAGAGAAC AGGCTCAAAT TCATAACACT 
GATGGACCAA AAAATGGAAA CACTAACTCC TTGAACTTAA GTTCACCAAA CCCTATGGAG 
AATAAGGGAA TGCCCTTTGA AATGGGCTTG GGGAACCCTT CCAGGAGCAC TGACACCCCT 
TCACAAAGCA CTGGAGAAAG AAAGACTGGG AGTGTTGGAT CTTGGGGTAC AGCTAGGGGG 
TCTTCTGGAA CTGACACAGT CTCTGGACAG AGCAATTCTG GAAACCATGG GAACAATGGA 
AAGGATAGGG AGGACTCCTG GAAAGGAGCT TCTGTTCAAA AACCTAATGG GTCAAGAAGC 
GATTCTTGGG ATAACAATAA CCGGTCTACG GGTGGTGGGT CTTGGAACTT TGGCCCTCAG 
CATGCAAATG AAAGCAAATG GGGTGAAGGG AACAAATTAA CATCTGGGGT CTCTCAGGGA 
GAATGGAAAC AGCTGTCTGG GTCTGATGAA CTGAAGATTG GAGAATGGAG TGGTCCAAAC 
CAACCAAATT CTAGCACTGG AGCATGGGAC AATCAAAAAG GCCACCCTCT TCCTGAAAAC 
CAAGGCAATT CCCAGGCTCC CTGTTGGGGA AGATCTTCCA GCTCTGCAGG AAGTGAGGTT 
GGAGGTCAAA GCACTGGAAG CAACCACAAA GCAGGAAGTA GTGACAGTCA CAATTCTGGA 
CGCCGATCAT ACAGGCCTAC GCATCCTGAT TGCCAGGCAG TCTTGCAGAC TTTGCTGAGC 
AGGACTGATT TGGACCCCCG AGTGCTTTCA AACACTGGCT GGGGCCAAAC TCAAATTAAG 
CAAGATACCG TATGGGATAT TGAAGAGATG CCACGGTCCG AGGGGAAGTC TGATAAAGGA 
ACTGAGGGGT GGGAGAGCTC TGCCACACAG ACAAAGAACT CAGGGGGCTG GGGCGATGTA 
CCCAGCCAAA GCAATCAAAT CAAGTCTGGA TGGGGTGAGC TCTCAACCCC AACAGAATGG 
AAGGACCCCA AAAATACTGG AGGATGGAAT GACTATAAGA ACCCCAATTC GTCTAATTGG 
GGAGGGGGAA GACAAGAAGA GAAATCGTCA TCTTCTTGGA ATGACAGCTC TAGTAAGGAT 
CAGGGGTGGG GTGGACGGCA GCCTAATCAA GGATGGTCTT CTGGAAAGAA TGGTTGGGGA 
GAGGAAGTTG ACCAAGCAAA AAACAGCAAC TGGGAAAGTG CGGGAAATAA GCCAGCATCT 
GGTTGGGGTG AAGGTGGGCA AAATGAGATT GGAACTTGGG GTAACGGTGC AAATACAGGC 
TCTGCTTCAA AGGGTGGTTG GGATGATTGT AAAAGAAATT CAACGTGGAA CGAGACGGGT 
CGACAGCCCA ACTCCTGGAA CAAGCAGCAG CAGCAGCAAC AGCAGCAACA GCAAGAGACT 
TCTGGTTCCT GGGGTCCACC GCCACAAACT CCAAGTAATG GGCGACCTCC AAACCCAAAC 
TGGAACAGCG GACCACAACC AGCTGCCCCC AAAGACGAGG AGCCTAGTGG CTGGGAAGAA 
CCTTCTCCAC AGTCAATCAG TCGAAAAATG GATATTGATG ATGGCACTTC AGCGTGGGGA 
GATCCTAGCA GTTATAACTA CAAGAATGTG AACCTGTGGG ACAAGAACTC ACAAGGGGGA 
CAGGCCCCAC GGGAACAGAG CCTGCCTACT CCAATGACTA GCAAATCACA AGCATCAGTC 
TGGAGCAAAA GCACTCCACC TGCTCCAGAT AATGGTACGT CCGCCTGGGG TGAGCCAAAT 
GAAACCAGTC CCGGGTGGGG TGAAGTAGAT GATACAGGAG CATCGACAAC AGGCTGGGGG 
AATGCACCTT CCAACGCCCC GAATGCCATG AAATCTAGTT CTAAATCTAT GCAAGATGGC 
TGGGGAGAGA GTGATGGGCC AGTGACAGGA ACACGTCATT CCAGCTGGGA AGAGGAGGAG 
GAGGGAGGAG TCTGGAACAC AGCAGGCTCT CAGGGAAGCA GTTCCTCCCA CAACTCAACA 
AGCTGGGGGC AAGGAGGAAA GAAAACGCAG ATGAAGTGCT CGTTAAAAGG AGGAAATAGT 
GATTCATGGA TGAACCCTAT ATCCAAGCAG TTTTCAAATA TGGGCTTGCT AAGTCAAACT 
GAGGACATTC CAGGCAATAA GATGGACTTG TCTGTAGGTG GTCTCCCAGA TAAGAAGTTT 
GAGGTAGATA AACGAGCCAT GAATCTCGGG GATTTTAATG ACATAATGAG AAAGGATCGA 
TCTGGGTTCC GTCCACCTAA TTCCAAAGAC ATGGGAACCA CAGATAGTGG GCCTTATTTT 
GAGAAGCTGA CTTTGCCTTT CTCCAATCAA GATGGGTGCC TTGGGGATGA GGCTCCCTGC 
TCTCCCTTCT CCCCTTCTCC CAGCTACAAG CTGTCTCCCT CTGGTTCCAC ACTATCCAAC 
GTCGGCCTTG GAGCAATCGG CTCAGGGCTC AGTCCCCAAA ACTACGCTGC TAGACAAGGT 
GGCAATCATG GTTTGTTTGG AAACAGCACA GCACAATCGA GGGGCATGCA CACACCAGTG 
CAGCCACTAA ATCCTTCCCC CAATATCCGG GCGCAAGTGC CTCCCCAATT TCTTTCTCCC 
CAGGTTTCGG CCTCCATGCT CAAACAGTTC CCCAACAGTG GCTTGAATCC AGGTCTTTTC 
AATGTGGGGC CCCAGCTTTC TCCACAACAG ATTGCCATGC TGAGCCAGCT TCCACAGATT 
CCCCAGTTTC AATTAGCATG TCAGCTCCTC CTTCAGCAGC AGCAGCAGCA GCAGCAGCTG 
TTACAGAGCC AGAGGAAGTT ATCTCAAGCT GTGCGACAGC AGCAGGAGCA ACAGATTGCT 
CGTATGGTGA GTGCCCTCCA GCAGCAGCAG CAGAGGCAGC CTGGCATGAA GCATTCACCA 
TCCCACCCTG TTGGGCCTAA GCCACATATA GACAGCATGG TACCCAATCC TTTGAATGTG 
GGTCTTTCAG ATTTACAGAC CAAAGGACAA ATACCTGGAT ACGGTTCAGG CTTTGGCTCA 
AGCAGCATGG ATTATGGCAT GGTGGGAGGG AAAGAAGCTG GAACAGAATC CCGCTTTAAA 
CAGTGGACTA TGATGGAAGG GCTGCCCTCT GTGGCTTCAC AAGATGCCAA TATGCACAAA 
AATGGTGCAA TAGTGCCCCC TGGAAAGGCT CGCGGAGGAT CGCCCTACAA CCAGTTCGAC 
ATAATTCAGG GCGACCCACT AGGTGGCCAT GCAGGCCCTG CTGGTGATAG TTGGTTACCT 
GCCAAATCTC CACCGACAAA CAAGATCGGA AGCAAATCCA GCAACGCCAG CTGGCCTCCA 
GAGTTCCAAC CAGGAGTGCC ATGGAAAGGT ATACAAAACA TTGACCCTGA ATCTGACCCC 
TACGTGACCC CTGGAAGTGT GCTGGGGGGG ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGACAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAATTCCT TCACCAACGT TCATAGCACT 
TCAGCAAAAT TCAGTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAATCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACAGGGCTG 
CCTCGCCCGC CTCCTGGCCT GACCAACCCC AAACCATCAT CTCCATGGAA CAGCACAGCA 
CCCCGATCGG TCAGGGGCTG GGGGGCACAG GACTCAAGGC TTGCCTCTGG TGAGAAGGAT 
CATCTGATAA CGCCATTGAT CAGACCTGAG GAGTGTGCAT TTTACAGCAC TAATGGTTTG 
GAGGAGAGAA AGTTGCAGTT GCCTGCATCT ACTTGGAGTG ATGGTAGCTC AGTTCGTCCT 
AGCTACTGGC TGGTTCTTCA CAATCTCACT CCACAGATCG ATGGGTCAAC CTTGAGGACG 
ATCTGCATGC AGCATGGTCC ACTGCTGACA TTCCATCTGA ACCTGACCCA GGGCACCGCT 
CTCATCCGAT ACAACACCAA ACAGGAGGCG GCCAAGGCCC AGACTGCACT GCACATGTGT 
GTGTTGGGAA ACACTACCAT CCTTGCTGAG TTTGCCACAG ATGAAGAGGT TAGTCGCTTT 
TTGACACAAG CTCAGCCCCC TACACCTGCA GCAACCCCAA GTGCACCCAC GGCAGGCTGG 
CAATCCCTGG AGACAGGACA GAACCAGACA GATCCAGTTG GACCTGCTTT GAATCTCTTT 
GGTGGGTCAA CAGGGCTTGG GCAGTGGAGC AGTGGTGGCG GCGGCAGCAG TGGGGGTGAT 
CTCACTGGCG CTTCGTTGTG GGGGCCCCCA AACTATTCTT CAAGCTTGTG GGGGGTCCCA 
AGCGTAGAGG ATCCACACAG AATGGGCAGC CCTGCTCCCT TACTACCTGG AGACCTTCTG 
GGAGGAGGGT CGGATTCAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_004937725.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004937782.1 Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OGa01770
Clone ID Related Accession (Same CDS sequence)	XM_004937725.2 , XM_004937725.3
Accession Version	XM_004937725.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	trinucleotide repeat-containing gene 6B protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455714.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004937725.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAGA AGGAGCAAGA AAGGGAAGAA CAGTTAATGG AAGACAAGAA AAGGAAGAAG 
GAGGATAAGA AAAAGAAGGA ATCTGCTCAG AAGGTCACAG ATCAAAAAAC CAAAGTGCCC 
GAAGTGACGA AACCAAGTTT AAGCCAACCA GTGGCTGCCA GCCCAATTGG CAACTCTCCA 
TCGCCACCAG TCAATGGTGG CAACAATGCC AAAAGGGTGG CAGTGCCGAA CGGACAACCG 
CCAAGCGCCG CCCGCTACAT GCCTCGGGAG GTGCCGCCGC GATTCCGTTG CCAGCAGGAC 
CACAAAGTGT TACTGAAACG TGGGCAGCCC CCTCCACCAT CCTGTATGCT CCTTGGGGGT 
GGAGCAGGGC CTCCTCCCCC TACAGCACCA GGAGCAAACC CAAACAACGC ACAACCAGTG 
ACAGGAGCAC TGCTGCAGAG CGACAGTGGG ACTGCAACAG ATTCAACAAT TGGAGGTGCT 
GCTGCTTCAA ATTATGCAAA TTCCACTTGG GGTCCTGGAG CCTCCTCCAG CAGCGGCACC 
AACGCCAACC CAACTCACAT CTGGGACAAG GTGATTGTAG ACGGGTCTGA CATGGAAGAG 
TGGCCTTGTA TCGCCAGCAA AGATGCTGAG TCTTCTTCCG AAAACACCAC CGATAACAAC 
AGTGCCTCGA ACCCTGGCTT GGAGAAGAAC ACTCTGCCAG GAAGCACCAC TAGTAACAAA 
GGAAAAGGAA GCCAGTGCCA GTCTGGAAGC GCTGGAAATG AATGTAATCT TGGGGCCTGG 
AAATCTGATC CCAAGGCTAA ATCTGTTCAA TCTTCCAACC CTGCTACCGA GAGTAACAGT 
GGACTAAGTA ATTGGAGGAA CTTGACTGGA CAAGATAGAA TTGGTTCTGG TTCTGGCTTC 
AGCAACTTTA ACCCAAATAG CAACCCGTCT GCTTGGCCAG CACTGGTCCA AGAGGGCAAT 
TCTAGGAAAG GGACTTCGGA ATCTGATAGT GGTAGCTCCA ATGCACAGAT TAGCACAGTA 
GGTCAGACCT CTAGGGAACA GCAGTCAAAG ATGGAAAATG CGGGTGTTAA CTTTGTCTCT 
GGCAGAGAAC AGGCTCAAAT TCATAACACT GATGGACCAA AAAATGGAAA CACTAACTCC 
TTGAACTTAA GTTCACCAAA CCCTATGGAG AATAAGGGAA TGCCCTTTGA AATGGGCTTG 
GGGAACCCTT CCAGGAGCAC TGACACCCCT TCACAAAGCA CTGGAGAAAG AAAGACTGGG 
AGTGTTGGAT CTTGGGGTAC AGCTAGGGGG TCTTCTGGAA CTGACACAGT CTCTGGACAG 
AGCAATTCTG GAAACCATGG GAACAATGGA AAGGATAGGG AGGACTCCTG GAAAGGAGCT 
TCTGTTCAAA AACCTAATGG GTCAAGAAGC GATTCTTGGG ATAACAATAA CCGGTCTACG 
GGTGGTGGGT CTTGGAACTT TGGCCCTCAG CATGCAAATG AAAGCAAATG GGGTGAAGGG 
AACAAATTAA CATCTGGGGT CTCTCAGGGA GAATGGAAAC AGCTGTCTGG GTCTGATGAA 
CTGAAGATTG GAGAATGGAG TGGTCCAAAC CAACCAAATT CTAGCACTGG AGCATGGGAC 
AATCAAAAAG GCCACCCTCT TCCTGAAAAC CAAGGCAATT CCCAGGCTCC CTGTTGGGGA 
AGATCTTCCA GCTCTGCAGG AAGTGAGGTT GGAGGTCAAA GCACTGGAAG CAACCACAAA 
GCAGGAAGTA GTGACAGTCA CAATTCTGGA CGCCGATCAT ACAGGCCTAC GCATCCTGAT 
TGCCAGGCAG TCTTGCAGAC TTTGCTGAGC AGGACTGATT TGGACCCCCG AGTGCTTTCA 
AACACTGGCT GGGGCCAAAC TCAAATTAAG CAAGATACCG TATGGGATAT TGAAGAGATG 
CCACGGTCCG AGGGGAAGTC TGATAAAGGA ACTGAGGGGT GGGAGAGCTC TGCCACACAG 
ACAAAGAACT CAGGGGGCTG GGGCGATGTA CCCAGCCAAA GCAATCAAAT CAAGTCTGGA 
TGGGGTGAGC TCTCAACCCC AACAGAATGG AAGGACCCCA AAAATACTGG AGGATGGAAT 
GACTATAAGA ACCCCAATTC GTCTAATTGG GGAGGGGGAA GACAAGAAGA GAAATCGTCA 
TCTTCTTGGA ATGACAGCTC TAGTAAGGAT CAGGGGTGGG GTGGACGGCA GCCTAATCAA 
GGATGGTCTT CTGGAAAGAA TGGTTGGGGA GAGGAAGTTG ACCAAGCAAA AAACAGCAAC 
TGGGAAAGTG CGGGAAATAA GCCAGCATCT GGTTGGGGTG AAGGTGGGCA AAATGAGATT 
GGAACTTGGG GTAACGGTGC AAATACAGGC TCTGCTTCAA AGGGTGGTTG GGATGATTGT 
AAAAGAAATT CAACGTGGAA CGAGACGGGT CGACAGCCCA ACTCCTGGAA CAAGCAGCAG 
CAGCAGCAAC AGCAGCAACA GCAAGAGACT TCTGGTTCCT GGGGTCCACC GCCACAAACT 
CCAAGTAATG GGCGACCTCC AAACCCAAAC TGGAACAGCG GACCACAACC AGCTGCCCCC 
AAAGACGAGG AGCCTAGTGG CTGGGAAGAA CCTTCTCCAC AGTCAATCAG TCGAAAAATG 
GATATTGATG ATGGCACTTC AGCGTGGGGA GATCCTAGCA GTTATAACTA CAAGAATGTG 
AACCTGTGGG ACAAGAACTC ACAAGGGGGA CAGGCCCCAC GGGAACAGAG CCTGCCTACT 
CCAATGACTA GCAAATCACA AGCATCAGTC TGGAGCAAAA GCACTCCACC TGCTCCAGAT 
AATGGTACGT CCGCCTGGGG TGAGCCAAAT GAAACCAGTC CCGGGTGGGG TGAAGTAGAT 
GATACAGGAG CATCGACAAC AGGCTGGGGG AATGCACCTT CCAACGCCCC GAATGCCATG 
AAATCTAGTT CTAAATCTAT GCAAGATGGC TGGGGAGAGA GTGATGGGCC AGTGACAGGA 
ACACGTCATT CCAGCTGGGA AGAGGAGGAG GAGGGAGGAG TCTGGAACAC AGCAGGCTCT 
CAGGGAAGCA GTTCCTCCCA CAACTCAACA AGCTGGGGGC AAGGAGGAAA GAAAACGCAG 
ATGAAGTGCT CGTTAAAAGG AGGAAATAGT GATTCATGGA TGAACCCTAT ATCCAAGCAG 
TTTTCAAATA TGGGCTTGCT AAGTCAAACT GAGGACATTC CAGGCAATAA GATGGACTTG 
TCTGTAGGTG GTCTCCCAGA TAAGAAGTTT GAGGTAGATA AACGAGCCAT GAATCTCGGG 
GATTTTAATG ACATAATGAG AAAGGATCGA TCTGGGTTCC GTCCACCTAA TTCCAAAGAC 
ATGGGAACCA CAGATAGTGG GCCTTATTTT GAGAAGCTGA CTTTGCCTTT CTCCAATCAA 
GATGGGTGCC TTGGGGATGA GGCTCCCTGC TCTCCCTTCT CCCCTTCTCC CAGCTACAAG 
CTGTCTCCCT CTGGTTCCAC ACTATCCAAC GTCGGCCTTG GAGCAATCGG CTCAGGGCTC 
AGTCCCCAAA ACTACGCTGC TAGACAAGGT GGCAATCATG GTTTGTTTGG AAACAGCACA 
GCACAATCGA GGGGCATGCA CACACCAGTG CAGCCACTAA ATCCTTCCCC CAATATCCGG 
GCGCAAGTGC CTCCCCAATT TCTTTCTCCC CAGGTTTCGG CCTCCATGCT CAAACAGTTC 
CCCAACAGTG GCTTGAATCC AGGTCTTTTC AATGTGGGGC CCCAGCTTTC TCCACAACAG 
ATTGCCATGC TGAGCCAGCT TCCACAGATT CCCCAGTTTC AATTAGCATG TCAGCTCCTC 
CTTCAGCAGC AGCAGCAGCA GCAGCAGCTG TTACAGAGCC AGAGGAAGTT ATCTCAAGCT 
GTGCGACAGC AGCAGGAGCA ACAGATTGCT CGTATGGTGA GTGCCCTCCA GCAGCAGCAG 
CAGAGGCAGC CTGGCATGAA GCATTCACCA TCCCACCCTG TTGGGCCTAA GCCACATATA 
GACAGCATGG TACCCAATCC TTTGAATGTG GGTCTTTCAG ATTTACAGAC CAAAGGACAA 
ATACCTGGAT ACGGTTCAGG CTTTGGCTCA AGCAGCATGG ATTATGGCAT GGTGGGAGGG 
AAAGAAGCTG GAACAGAATC CCGCTTTAAA CAGTGGACTA TGATGGAAGG GCTGCCCTCT 
GTGGCTTCAC AAGATGCCAA TATGCACAAA AATGGTGCAA TAGTGCCCCC TGGAAAGGCT 
CGCGGAGGAT CGCCCTACAA CCAGTTCGAC ATAATTCAGG GCGACCCACT AGGTGGCCAT 
GCAGGCCCTG CTGGTGATAG TTGGTTACCT GCCAAATCTC CACCGACAAA CAAGATCGGA 
AGCAAATCCA GCAACGCCAG CTGGCCTCCA GAGTTCCAAC CAGGAGTGCC ATGGAAAGGT 
ATACAAAACA TTGACCCTGA ATCTGACCCC TACGTGACCC CTGGAAGTGT GCTGGGGGGG 
ACAGCCACAT CTCCCATTGT AGATACTGAC CACCAACTTC TGCGGGACAA CACCACAGGG 
TCTAATTCTT CCCTCAACAC CTCGCTGCCT TCACCTGGTG CCTGGCCCTA CAGTGCCTCT 
GACAATTCCT TCACCAACGT TCATAGCACT TCAGCAAAAT TCAGTGATTA CAAATCAACA 
TGGTCCCCAG ATCCCATAGG ACACAATCCC ACTCATCTCT CCAACAAGAT GTGGAAAAAC 
CATATTTCCT CAAGGAACAC TACAGGGCTG CCTCGCCCGC CTCCTGGCCT GACCAACCCC 
AAACCATCAT CTCCATGGAA CAGCACAGCA CCCCGATCGG TCAGGGGCTG GGGGGCACAG 
GACTCAAGGC TTGCCTCTGG TGAGAAGGAT CATCTGATAA CGCCATTGAT CAGACCTGAG 
GAGTGTGCAT TTTACAGCAC TAATGGTTTG GAGGAGAGAA AGTTGCAGTT GCCTGCATCT 
ACTTGGAGTG ATGGTAGCTC AGTTCGTCCT AGCTACTGGC TGGTTCTTCA CAATCTCACT 
CCACAGATCG ATGGGTCAAC CTTGAGGACG ATCTGCATGC AGCATGGTCC ACTGCTGACA 
TTCCATCTGA ACCTGACCCA GGGCACCGCT CTCATCCGAT ACAACACCAA ACAGGAGGCG 
GCCAAGGCCC AGACTGCACT GCACATGTGT GTGTTGGGAA ACACTACCAT CCTTGCTGAG 
TTTGCCACAG ATGAAGAGGT TAGTCGCTTT TTGACACAAG CTCAGCCCCC TACACCTGCA 
GCAACCCCAA GTGCACCCAC GGCAGGCTGG CAATCCCTGG AGACAGGACA GAACCAGACA 
GATCCAGTTG GACCTGCTTT GAATCTCTTT GGTGGGTCAA CAGGGCTTGG GCAGTGGAGC 
AGTGGTGGCG GCGGCAGCAG TGGGGGTGAT CTCACTGGCG CTTCGTTGTG GGGGCCCCCA 
AACTATTCTT CAAGCTTGTG GGGGGTCCCA AGCGTAGAGG ATCCACACAG AATGGGCAGC 
CCTGCTCCCT TACTACCTGG AGACCTTCTG GGAGGAGGGT CGGATTCAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004937782.1
CDS	221..5794
Translation	MREKEQEREEQLMEDKKRKKEDKKKKESAQKVTDQKTKVPEVTKPSLSQPVAASPIGNSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPPTAPGANPNNAQPVTGALLQSDSGTATDSTIGGAAASNYANSTWGPGASSSSGTNANPTHIWDKVIVDGSDMEEWPCIASKDAESSSENTTDNNSASNPGLEKNTLPGSTTSNKGKGSQCQSGSAGNECNLGAWKSDPKAKSVQSSNPATESNSGLSNWRNLTGQDRIGSGSGFSNFNPNSNPSAWPALVQEGNSRKGTSESDSGSSNAQISTVGQTSREQQSKMENAGVNFVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFEMGLGNPSRSTDTPSQSTGERKTGSVGSWGTARGSSGTDTVSGQSNSGNHGNNGKDREDSWKGASVQKPNGSRSDSWDNNNRSTGGGSWNFGPQHANESKWGEGNKLTSGVSQGEWKQLSGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNSQAPCWGRSSSSAGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEMPRSEGKSDKGTEGWESSATQTKNSGGWGDVPSQSNQIKSGWGELSTPTEWKDPKNTGGWNDYKNPNSSNWGGGRQEEKSSSSWNDSSSKDQGWGGRQPNQGWSSGKNGWGEEVDQAKNSNWESAGNKPASGWGEGGQNEIGTWGNGANTGSASKGGWDDCKRNSTWNETGRQPNSWNKQQQQQQQQQQETSGSWGPPPQTPSNGRPPNPNWNSGPQPAAPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPSSYNYKNVNLWDKNSQGGQAPREQSLPTPMTSKSQASVWSKSTPPAPDNGTSAWGEPNETSPGWGEVDDTGASTTGWGNAPSNAPNAMKSSSKSMQDGWGESDGPVTGTRHSSWEEEEEGGVWNTAGSQGSSSSHNSTSWGQGGKKTQMKCSLKGGNSDSWMNPISKQFSNMGLLSQTEDIPGNKMDLSVGGLPDKKFEVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLSNVGLGAIGSGLSPQNYAARQGGNHGLFGNSTAQSRGMHTPVQPLNPSPNIRAQVPPQFLSPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQQLLQSQRKLSQAVRQQQEQQIARMVSALQQQQQRQPGMKHSPSHPVGPKPHIDSMVPNPLNVGLSDLQTKGQIPGYGSGFGSSSMDYGMVGGKEAGTESRFKQWTMMEGLPSVASQDANMHKNGAIVPPGKARGGSPYNQFDIIQGDPLGGHAGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFSDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTGLPRPPPGLTNPKPSSPWNSTAPRSVRGWGAQDSRLASGEKDHLITPLIRPEECAFYSTNGLEERKLQLPASTWSDGSSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYNTKQEAAKAQTALHMCVLGNTTILAEFATDEEVSRFLTQAQPPTPAATPSAPTAGWQSLETGQNQTDPVGPALNLFGGSTGLGQWSSGGGGSSGGDLTGASLWGPPNYSSSLWGVPSVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_004937725.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004937725.2

ORF Insert Sequence:

ATGAGAGAGA AGGAGCAAGA AAGGGAAGAA CAGTTAATGG AAGACAAGAA AAGGAAGAAG 
GAGGATAAGA AAAAGAAGGA ATCTGCTCAG AAGGTCACAG ATCAAAAAAC CAAAGTGCCC 
GAAGTGACGA AACCAAGTTT AAGCCAACCA GTGGCTGCCA GCCCAATTGG CAACTCTCCA 
TCGCCACCAG TCAATGGTGG CAACAATGCC AAAAGGGTGG CAGTGCCGAA CGGACAACCG 
CCAAGCGCCG CCCGCTACAT GCCTCGGGAG GTGCCGCCGC GATTCCGTTG CCAGCAGGAC 
CACAAAGTGT TACTGAAACG TGGGCAGCCC CCTCCACCAT CCTGTATGCT CCTTGGGGGT 
GGAGCAGGGC CTCCTCCCCC TACAGCACCA GGAGCAAACC CAAACAACGC ACAACCAGTG 
ACAGGAGCAC TGCTGCAGAG CGACAGTGGG ACTGCAACAG ATTCAACAAT TGGAGGTGCT 
GCTGCTTCAA ATTATGCAAA TTCCACTTGG GGTCCTGGAG CCTCCTCCAG CAGCGGCACC 
AACGCCAACC CAACTCACAT CTGGGACAAG GTGATTGTAG ACGGGTCTGA CATGGAAGAG 
TGGCCTTGTA TCGCCAGCAA AGATGCTGAG TCTTCTTCCG AAAACACCAC CGATAACAAC 
AGTGCCTCGA ACCCTGGCTT GGAGAAGAAC ACTCTGCCAG GAAGCACCAC TAGTAACAAA 
GGAAAAGGAA GCCAGTGCCA GTCTGGAAGC GCTGGAAATG AATGTAATCT TGGGGCCTGG 
AAATCTGATC CCAAGGCTAA ATCTGTTCAA TCTTCCAACC CTGCTACCGA GAGTAACAGT 
GGACTAAGTA ATTGGAGGAA CTTGACTGGA CAAGATAGAA TTGGTTCTGG TTCTGGCTTC 
AGCAACTTTA ACCCAAATAG CAACCCGTCT GCTTGGCCAG CACTGGTCCA AGAGGGCAAT 
TCTAGGAAAG GGACTTCGGA ATCTGATAGT GGTAGCTCCA ATGCACAGAT TAGCACAGTA 
GGTCAGACCT CTAGGGAACA GCAGTCAAAG ATGGAAAATG CGGGTGTTAA CTTTGTCTCT 
GGCAGAGAAC AGGCTCAAAT TCATAACACT GATGGACCAA AAAATGGAAA CACTAACTCC 
TTGAACTTAA GTTCACCAAA CCCTATGGAG AATAAGGGAA TGCCCTTTGA AATGGGCTTG 
GGGAACCCTT CCAGGAGCAC TGACACCCCT TCACAAAGCA CTGGAGAAAG AAAGACTGGG 
AGTGTTGGAT CTTGGGGTAC AGCTAGGGGG TCTTCTGGAA CTGACACAGT CTCTGGACAG 
AGCAATTCTG GAAACCATGG GAACAATGGA AAGGATAGGG AGGACTCCTG GAAAGGAGCT 
TCTGTTCAAA AACCTAATGG GTCAAGAAGC GATTCTTGGG ATAACAATAA CCGGTCTACG 
GGTGGTGGGT CTTGGAACTT TGGCCCTCAG CATGCAAATG AAAGCAAATG GGGTGAAGGG 
AACAAATTAA CATCTGGGGT CTCTCAGGGA GAATGGAAAC AGCTGTCTGG GTCTGATGAA 
CTGAAGATTG GAGAATGGAG TGGTCCAAAC CAACCAAATT CTAGCACTGG AGCATGGGAC 
AATCAAAAAG GCCACCCTCT TCCTGAAAAC CAAGGCAATT CCCAGGCTCC CTGTTGGGGA 
AGATCTTCCA GCTCTGCAGG AAGTGAGGTT GGAGGTCAAA GCACTGGAAG CAACCACAAA 
GCAGGAAGTA GTGACAGTCA CAATTCTGGA CGCCGATCAT ACAGGCCTAC GCATCCTGAT 
TGCCAGGCAG TCTTGCAGAC TTTGCTGAGC AGGACTGATT TGGACCCCCG AGTGCTTTCA 
AACACTGGCT GGGGCCAAAC TCAAATTAAG CAAGATACCG TATGGGATAT TGAAGAGATG 
CCACGGTCCG AGGGGAAGTC TGATAAAGGA ACTGAGGGGT GGGAGAGCTC TGCCACACAG 
ACAAAGAACT CAGGGGGCTG GGGCGATGTA CCCAGCCAAA GCAATCAAAT CAAGTCTGGA 
TGGGGTGAGC TCTCAACCCC AACAGAATGG AAGGACCCCA AAAATACTGG AGGATGGAAT 
GACTATAAGA ACCCCAATTC GTCTAATTGG GGAGGGGGAA GACAAGAAGA GAAATCGTCA 
TCTTCTTGGA ATGACAGCTC TAGTAAGGAT CAGGGGTGGG GTGGACGGCA GCCTAATCAA 
GGATGGTCTT CTGGAAAGAA TGGTTGGGGA GAGGAAGTTG ACCAAGCAAA AAACAGCAAC 
TGGGAAAGTG CGGGAAATAA GCCAGCATCT GGTTGGGGTG AAGGTGGGCA AAATGAGATT 
GGAACTTGGG GTAACGGTGC AAATACAGGC TCTGCTTCAA AGGGTGGTTG GGATGATTGT 
AAAAGAAATT CAACGTGGAA CGAGACGGGT CGACAGCCCA ACTCCTGGAA CAAGCAGCAG 
CAGCAGCAAC AGCAGCAACA GCAAGAGACT TCTGGTTCCT GGGGTCCACC GCCACAAACT 
CCAAGTAATG GGCGACCTCC AAACCCAAAC TGGAACAGCG GACCACAACC AGCTGCCCCC 
AAAGACGAGG AGCCTAGTGG CTGGGAAGAA CCTTCTCCAC AGTCAATCAG TCGAAAAATG 
GATATTGATG ATGGCACTTC AGCGTGGGGA GATCCTAGCA GTTATAACTA CAAGAATGTG 
AACCTGTGGG ACAAGAACTC ACAAGGGGGA CAGGCCCCAC GGGAACAGAG CCTGCCTACT 
CCAATGACTA GCAAATCACA AGCATCAGTC TGGAGCAAAA GCACTCCACC TGCTCCAGAT 
AATGGTACGT CCGCCTGGGG TGAGCCAAAT GAAACCAGTC CCGGGTGGGG TGAAGTAGAT 
GATACAGGAG CATCGACAAC AGGCTGGGGG AATGCACCTT CCAACGCCCC GAATGCCATG 
AAATCTAGTT CTAAATCTAT GCAAGATGGC TGGGGAGAGA GTGATGGGCC AGTGACAGGA 
ACACGTCATT CCAGCTGGGA AGAGGAGGAG GAGGGAGGAG TCTGGAACAC AGCAGGCTCT 
CAGGGAAGCA GTTCCTCCCA CAACTCAACA AGCTGGGGGC AAGGAGGAAA GAAAACGCAG 
ATGAAGTGCT CGTTAAAAGG AGGAAATAGT GATTCATGGA TGAACCCTAT ATCCAAGCAG 
TTTTCAAATA TGGGCTTGCT AAGTCAAACT GAGGACATTC CAGGCAATAA GATGGACTTG 
TCTGTAGGTG GTCTCCCAGA TAAGAAGTTT GAGGTAGATA AACGAGCCAT GAATCTCGGG 
GATTTTAATG ACATAATGAG AAAGGATCGA TCTGGGTTCC GTCCACCTAA TTCCAAAGAC 
ATGGGAACCA CAGATAGTGG GCCTTATTTT GAGAAGCTGA CTTTGCCTTT CTCCAATCAA 
GATGGGTGCC TTGGGGATGA GGCTCCCTGC TCTCCCTTCT CCCCTTCTCC CAGCTACAAG 
CTGTCTCCCT CTGGTTCCAC ACTATCCAAC GTCGGCCTTG GAGCAATCGG CTCAGGGCTC 
AGTCCCCAAA ACTACGCTGC TAGACAAGGT GGCAATCATG GTTTGTTTGG AAACAGCACA 
GCACAATCGA GGGGCATGCA CACACCAGTG CAGCCACTAA ATCCTTCCCC CAATATCCGG 
GCGCAAGTGC CTCCCCAATT TCTTTCTCCC CAGGTTTCGG CCTCCATGCT CAAACAGTTC 
CCCAACAGTG GCTTGAATCC AGGTCTTTTC AATGTGGGGC CCCAGCTTTC TCCACAACAG 
ATTGCCATGC TGAGCCAGCT TCCACAGATT CCCCAGTTTC AATTAGCATG TCAGCTCCTC 
CTTCAGCAGC AGCAGCAGCA GCAGCAGCTG TTACAGAGCC AGAGGAAGTT ATCTCAAGCT 
GTGCGACAGC AGCAGGAGCA ACAGATTGCT CGTATGGTGA GTGCCCTCCA GCAGCAGCAG 
CAGAGGCAGC CTGGCATGAA GCATTCACCA TCCCACCCTG TTGGGCCTAA GCCACATATA 
GACAGCATGG TACCCAATCC TTTGAATGTG GGTCTTTCAG ATTTACAGAC CAAAGGACAA 
ATACCTGGAT ACGGTTCAGG CTTTGGCTCA AGCAGCATGG ATTATGGCAT GGTGGGAGGG 
AAAGAAGCTG GAACAGAATC CCGCTTTAAA CAGTGGACTA TGATGGAAGG GCTGCCCTCT 
GTGGCTTCAC AAGATGCCAA TATGCACAAA AATGGTGCAA TAGTGCCCCC TGGAAAGGCT 
CGCGGAGGAT CGCCCTACAA CCAGTTCGAC ATAATTCAGG GCGACCCACT AGGTGGCCAT 
GCAGGCCCTG CTGGTGATAG TTGGTTACCT GCCAAATCTC CACCGACAAA CAAGATCGGA 
AGCAAATCCA GCAACGCCAG CTGGCCTCCA GAGTTCCAAC CAGGAGTGCC ATGGAAAGGT 
ATACAAAACA TTGACCCTGA ATCTGACCCC TACGTGACCC CTGGAAGTGT GCTGGGGGGG 
ACAGCCACAT CTCCCATTGT AGATACTGAC CACCAACTTC TGCGGGACAA CACCACAGGG 
TCTAATTCTT CCCTCAACAC CTCGCTGCCT TCACCTGGTG CCTGGCCCTA CAGTGCCTCT 
GACAATTCCT TCACCAACGT TCATAGCACT TCAGCAAAAT TCAGTGATTA CAAATCAACA 
TGGTCCCCAG ATCCCATAGG ACACAATCCC ACTCATCTCT CCAACAAGAT GTGGAAAAAC 
CATATTTCCT CAAGGAACAC TACAGGGCTG CCTCGCCCGC CTCCTGGCCT GACCAACCCC 
AAACCATCAT CTCCATGGAA CAGCACAGCA CCCCGATCGG TCAGGGGCTG GGGGGCACAG 
GACTCAAGGC TTGCCTCTGG TGAGAAGGAT CATCTGATAA CGCCATTGAT CAGACCTGAG 
GAGTGTGCAT TTTACAGCAC TAATGGTTTG GAGGAGAGAA AGTTGCAGTT GCCTGCATCT 
ACTTGGAGTG ATGGTAGCTC AGTTCGTCCT AGCTACTGGC TGGTTCTTCA CAATCTCACT 
CCACAGATCG ATGGGTCAAC CTTGAGGACG ATCTGCATGC AGCATGGTCC ACTGCTGACA 
TTCCATCTGA ACCTGACCCA GGGCACCGCT CTCATCCGAT ACAACACCAA ACAGGAGGCG 
GCCAAGGCCC AGACTGCACT GCACATGTGT GTGTTGGGAA ACACTACCAT CCTTGCTGAG 
TTTGCCACAG ATGAAGAGGT TAGTCGCTTT TTGACACAAG CTCAGCCCCC TACACCTGCA 
GCAACCCCAA GTGCACCCAC GGCAGGCTGG CAATCCCTGG AGACAGGACA GAACCAGACA 
GATCCAGTTG GACCTGCTTT GAATCTCTTT GGTGGGTCAA CAGGGCTTGG GCAGTGGAGC 
AGTGGTGGCG GCGGCAGCAG TGGGGGTGAT CTCACTGGCG CTTCGTTGTG GGGGCCCCCA 
AACTATTCTT CAAGCTTGTG GGGGGTCCCA AGCGTAGAGG ATCCACACAG AATGGGCAGC 
CCTGCTCCCT TACTACCTGG AGACCTTCTG GGAGGAGGGT CGGATTCAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_004937725.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004937782.1 Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X3, mRNA.

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CloneID	OGa01770
Clone ID Related Accession (Same CDS sequence)	XM_004937725.2 , XM_004937725.3
Accession Version	XM_004937725.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	trinucleotide repeat-containing gene 6B protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004937725.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAGA AGGAGCAAGA AAGGGAAGAA CAGTTAATGG AAGACAAGAA AAGGAAGAAG 
GAGGATAAGA AAAAGAAGGA ATCTGCTCAG AAGGTCACAG ATCAAAAAAC CAAAGTGCCC 
GAAGTGACGA AACCAAGTTT AAGCCAACCA GTGGCTGCCA GCCCAATTGG CAACTCTCCA 
TCGCCACCAG TCAATGGTGG CAACAATGCC AAAAGGGTGG CAGTGCCGAA CGGACAACCG 
CCAAGCGCCG CCCGCTACAT GCCTCGGGAG GTGCCGCCGC GATTCCGTTG CCAGCAGGAC 
CACAAAGTGT TACTGAAACG TGGGCAGCCC CCTCCACCAT CCTGTATGCT CCTTGGGGGT 
GGAGCAGGGC CTCCTCCCCC TACAGCACCA GGAGCAAACC CAAACAACGC ACAACCAGTG 
ACAGGAGCAC TGCTGCAGAG CGACAGTGGG ACTGCAACAG ATTCAACAAT TGGAGGTGCT 
GCTGCTTCAA ATTATGCAAA TTCCACTTGG GGTCCTGGAG CCTCCTCCAG CAGCGGCACC 
AACGCCAACC CAACTCACAT CTGGGACAAG GTGATTGTAG ACGGGTCTGA CATGGAAGAG 
TGGCCTTGTA TCGCCAGCAA AGATGCTGAG TCTTCTTCCG AAAACACCAC CGATAACAAC 
AGTGCCTCGA ACCCTGGCTT GGAGAAGAAC ACTCTGCCAG GAAGCACCAC TAGTAACAAA 
GGAAAAGGAA GCCAGTGCCA GTCTGGAAGC GCTGGAAATG AATGTAATCT TGGGGCCTGG 
AAATCTGATC CCAAGGCTAA ATCTGTTCAA TCTTCCAACC CTGCTACCGA GAGTAACAGT 
GGACTAAGTA ATTGGAGGAA CTTGACTGGA CAAGATAGAA TTGGTTCTGG TTCTGGCTTC 
AGCAACTTTA ACCCAAATAG CAACCCGTCT GCTTGGCCAG CACTGGTCCA AGAGGGCAAT 
TCTAGGAAAG GGACTTCGGA ATCTGATAGT GGTAGCTCCA ATGCACAGAT TAGCACAGTA 
GGTCAGACCT CTAGGGAACA GCAGTCAAAG ATGGAAAATG CGGGTGTTAA CTTTGTCTCT 
GGCAGAGAAC AGGCTCAAAT TCATAACACT GATGGACCAA AAAATGGAAA CACTAACTCC 
TTGAACTTAA GTTCACCAAA CCCTATGGAG AATAAGGGAA TGCCCTTTGA AATGGGCTTG 
GGGAACCCTT CCAGGAGCAC TGACACCCCT TCACAAAGCA CTGGAGAAAG AAAGACTGGG 
AGTGTTGGAT CTTGGGGTAC AGCTAGGGGG TCTTCTGGAA CTGACACAGT CTCTGGACAG 
AGCAATTCTG GAAACCATGG GAACAATGGA AAGGATAGGG AGGACTCCTG GAAAGGAGCT 
TCTGTTCAAA AACCTAATGG GTCAAGAAGC GATTCTTGGG ATAACAATAA CCGGTCTACG 
GGTGGTGGGT CTTGGAACTT TGGCCCTCAG CATGCAAATG AAAGCAAATG GGGTGAAGGG 
AACAAATTAA CATCTGGGGT CTCTCAGGGA GAATGGAAAC AGCTGTCTGG GTCTGATGAA 
CTGAAGATTG GAGAATGGAG TGGTCCAAAC CAACCAAATT CTAGCACTGG AGCATGGGAC 
AATCAAAAAG GCCACCCTCT TCCTGAAAAC CAAGGCAATT CCCAGGCTCC CTGTTGGGGA 
AGATCTTCCA GCTCTGCAGG AAGTGAGGTT GGAGGTCAAA GCACTGGAAG CAACCACAAA 
GCAGGAAGTA GTGACAGTCA CAATTCTGGA CGCCGATCAT ACAGGCCTAC GCATCCTGAT 
TGCCAGGCAG TCTTGCAGAC TTTGCTGAGC AGGACTGATT TGGACCCCCG AGTGCTTTCA 
AACACTGGCT GGGGCCAAAC TCAAATTAAG CAAGATACCG TATGGGATAT TGAAGAGATG 
CCACGGTCCG AGGGGAAGTC TGATAAAGGA ACTGAGGGGT GGGAGAGCTC TGCCACACAG 
ACAAAGAACT CAGGGGGCTG GGGCGATGTA CCCAGCCAAA GCAATCAAAT CAAGTCTGGA 
TGGGGTGAGC TCTCAACCCC AACAGAATGG AAGGACCCCA AAAATACTGG AGGATGGAAT 
GACTATAAGA ACCCCAATTC GTCTAATTGG GGAGGGGGAA GACAAGAAGA GAAATCGTCA 
TCTTCTTGGA ATGACAGCTC TAGTAAGGAT CAGGGGTGGG GTGGACGGCA GCCTAATCAA 
GGATGGTCTT CTGGAAAGAA TGGTTGGGGA GAGGAAGTTG ACCAAGCAAA AAACAGCAAC 
TGGGAAAGTG CGGGAAATAA GCCAGCATCT GGTTGGGGTG AAGGTGGGCA AAATGAGATT 
GGAACTTGGG GTAACGGTGC AAATACAGGC TCTGCTTCAA AGGGTGGTTG GGATGATTGT 
AAAAGAAATT CAACGTGGAA CGAGACGGGT CGACAGCCCA ACTCCTGGAA CAAGCAGCAG 
CAGCAGCAAC AGCAGCAACA GCAAGAGACT TCTGGTTCCT GGGGTCCACC GCCACAAACT 
CCAAGTAATG GGCGACCTCC AAACCCAAAC TGGAACAGCG GACCACAACC AGCTGCCCCC 
AAAGACGAGG AGCCTAGTGG CTGGGAAGAA CCTTCTCCAC AGTCAATCAG TCGAAAAATG 
GATATTGATG ATGGCACTTC AGCGTGGGGA GATCCTAGCA GTTATAACTA CAAGAATGTG 
AACCTGTGGG ACAAGAACTC ACAAGGGGGA CAGGCCCCAC GGGAACAGAG CCTGCCTACT 
CCAATGACTA GCAAATCACA AGCATCAGTC TGGAGCAAAA GCACTCCACC TGCTCCAGAT 
AATGGTACGT CCGCCTGGGG TGAGCCAAAT GAAACCAGTC CCGGGTGGGG TGAAGTAGAT 
GATACAGGAG CATCGACAAC AGGCTGGGGG AATGCACCTT CCAACGCCCC GAATGCCATG 
AAATCTAGTT CTAAATCTAT GCAAGATGGC TGGGGAGAGA GTGATGGGCC AGTGACAGGA 
ACACGTCATT CCAGCTGGGA AGAGGAGGAG GAGGGAGGAG TCTGGAACAC AGCAGGCTCT 
CAGGGAAGCA GTTCCTCCCA CAACTCAACA AGCTGGGGGC AAGGAGGAAA GAAAACGCAG 
ATGAAGTGCT CGTTAAAAGG AGGAAATAGT GATTCATGGA TGAACCCTAT ATCCAAGCAG 
TTTTCAAATA TGGGCTTGCT AAGTCAAACT GAGGACATTC CAGGCAATAA GATGGACTTG 
TCTGTAGGTG GTCTCCCAGA TAAGAAGTTT GAGGTAGATA AACGAGCCAT GAATCTCGGG 
GATTTTAATG ACATAATGAG AAAGGATCGA TCTGGGTTCC GTCCACCTAA TTCCAAAGAC 
ATGGGAACCA CAGATAGTGG GCCTTATTTT GAGAAGCTGA CTTTGCCTTT CTCCAATCAA 
GATGGGTGCC TTGGGGATGA GGCTCCCTGC TCTCCCTTCT CCCCTTCTCC CAGCTACAAG 
CTGTCTCCCT CTGGTTCCAC ACTATCCAAC GTCGGCCTTG GAGCAATCGG CTCAGGGCTC 
AGTCCCCAAA ACTACGCTGC TAGACAAGGT GGCAATCATG GTTTGTTTGG AAACAGCACA 
GCACAATCGA GGGGCATGCA CACACCAGTG CAGCCACTAA ATCCTTCCCC CAATATCCGG 
GCGCAAGTGC CTCCCCAATT TCTTTCTCCC CAGGTTTCGG CCTCCATGCT CAAACAGTTC 
CCCAACAGTG GCTTGAATCC AGGTCTTTTC AATGTGGGGC CCCAGCTTTC TCCACAACAG 
ATTGCCATGC TGAGCCAGCT TCCACAGATT CCCCAGTTTC AATTAGCATG TCAGCTCCTC 
CTTCAGCAGC AGCAGCAGCA GCAGCAGCTG TTACAGAGCC AGAGGAAGTT ATCTCAAGCT 
GTGCGACAGC AGCAGGAGCA ACAGATTGCT CGTATGGTGA GTGCCCTCCA GCAGCAGCAG 
CAGAGGCAGC CTGGCATGAA GCATTCACCA TCCCACCCTG TTGGGCCTAA GCCACATATA 
GACAGCATGG TACCCAATCC TTTGAATGTG GGTCTTTCAG ATTTACAGAC CAAAGGACAA 
ATACCTGGAT ACGGTTCAGG CTTTGGCTCA AGCAGCATGG ATTATGGCAT GGTGGGAGGG 
AAAGAAGCTG GAACAGAATC CCGCTTTAAA CAGTGGACTA TGATGGAAGG GCTGCCCTCT 
GTGGCTTCAC AAGATGCCAA TATGCACAAA AATGGTGCAA TAGTGCCCCC TGGAAAGGCT 
CGCGGAGGAT CGCCCTACAA CCAGTTCGAC ATAATTCAGG GCGACCCACT AGGTGGCCAT 
GCAGGCCCTG CTGGTGATAG TTGGTTACCT GCCAAATCTC CACCGACAAA CAAGATCGGA 
AGCAAATCCA GCAACGCCAG CTGGCCTCCA GAGTTCCAAC CAGGAGTGCC ATGGAAAGGT 
ATACAAAACA TTGACCCTGA ATCTGACCCC TACGTGACCC CTGGAAGTGT GCTGGGGGGG 
ACAGCCACAT CTCCCATTGT AGATACTGAC CACCAACTTC TGCGGGACAA CACCACAGGG 
TCTAATTCTT CCCTCAACAC CTCGCTGCCT TCACCTGGTG CCTGGCCCTA CAGTGCCTCT 
GACAATTCCT TCACCAACGT TCATAGCACT TCAGCAAAAT TCAGTGATTA CAAATCAACA 
TGGTCCCCAG ATCCCATAGG ACACAATCCC ACTCATCTCT CCAACAAGAT GTGGAAAAAC 
CATATTTCCT CAAGGAACAC TACAGGGCTG CCTCGCCCGC CTCCTGGCCT GACCAACCCC 
AAACCATCAT CTCCATGGAA CAGCACAGCA CCCCGATCGG TCAGGGGCTG GGGGGCACAG 
GACTCAAGGC TTGCCTCTGG TGAGAAGGAT CATCTGATAA CGCCATTGAT CAGACCTGAG 
GAGTGTGCAT TTTACAGCAC TAATGGTTTG GAGGAGAGAA AGTTGCAGTT GCCTGCATCT 
ACTTGGAGTG ATGGTAGCTC AGTTCGTCCT AGCTACTGGC TGGTTCTTCA CAATCTCACT 
CCACAGATCG ATGGGTCAAC CTTGAGGACG ATCTGCATGC AGCATGGTCC ACTGCTGACA 
TTCCATCTGA ACCTGACCCA GGGCACCGCT CTCATCCGAT ACAACACCAA ACAGGAGGCG 
GCCAAGGCCC AGACTGCACT GCACATGTGT GTGTTGGGAA ACACTACCAT CCTTGCTGAG 
TTTGCCACAG ATGAAGAGGT TAGTCGCTTT TTGACACAAG CTCAGCCCCC TACACCTGCA 
GCAACCCCAA GTGCACCCAC GGCAGGCTGG CAATCCCTGG AGACAGGACA GAACCAGACA 
GATCCAGTTG GACCTGCTTT GAATCTCTTT GGTGGGTCAA CAGGGCTTGG GCAGTGGAGC 
AGTGGTGGCG GCGGCAGCAG TGGGGGTGAT CTCACTGGCG CTTCGTTGTG GGGGCCCCCA 
AACTATTCTT CAAGCTTGTG GGGGGTCCCA AGCGTAGAGG ATCCACACAG AATGGGCAGC 
CCTGCTCCCT TACTACCTGG AGACCTTCTG GGAGGAGGGT CGGATTCAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004937782.1
CDS	226..5799
Translation	MREKEQEREEQLMEDKKRKKEDKKKKESAQKVTDQKTKVPEVTKPSLSQPVAASPIGNSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPPTAPGANPNNAQPVTGALLQSDSGTATDSTIGGAAASNYANSTWGPGASSSSGTNANPTHIWDKVIVDGSDMEEWPCIASKDAESSSENTTDNNSASNPGLEKNTLPGSTTSNKGKGSQCQSGSAGNECNLGAWKSDPKAKSVQSSNPATESNSGLSNWRNLTGQDRIGSGSGFSNFNPNSNPSAWPALVQEGNSRKGTSESDSGSSNAQISTVGQTSREQQSKMENAGVNFVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFEMGLGNPSRSTDTPSQSTGERKTGSVGSWGTARGSSGTDTVSGQSNSGNHGNNGKDREDSWKGASVQKPNGSRSDSWDNNNRSTGGGSWNFGPQHANESKWGEGNKLTSGVSQGEWKQLSGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNSQAPCWGRSSSSAGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEMPRSEGKSDKGTEGWESSATQTKNSGGWGDVPSQSNQIKSGWGELSTPTEWKDPKNTGGWNDYKNPNSSNWGGGRQEEKSSSSWNDSSSKDQGWGGRQPNQGWSSGKNGWGEEVDQAKNSNWESAGNKPASGWGEGGQNEIGTWGNGANTGSASKGGWDDCKRNSTWNETGRQPNSWNKQQQQQQQQQQETSGSWGPPPQTPSNGRPPNPNWNSGPQPAAPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPSSYNYKNVNLWDKNSQGGQAPREQSLPTPMTSKSQASVWSKSTPPAPDNGTSAWGEPNETSPGWGEVDDTGASTTGWGNAPSNAPNAMKSSSKSMQDGWGESDGPVTGTRHSSWEEEEEGGVWNTAGSQGSSSSHNSTSWGQGGKKTQMKCSLKGGNSDSWMNPISKQFSNMGLLSQTEDIPGNKMDLSVGGLPDKKFEVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLSNVGLGAIGSGLSPQNYAARQGGNHGLFGNSTAQSRGMHTPVQPLNPSPNIRAQVPPQFLSPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQQLLQSQRKLSQAVRQQQEQQIARMVSALQQQQQRQPGMKHSPSHPVGPKPHIDSMVPNPLNVGLSDLQTKGQIPGYGSGFGSSSMDYGMVGGKEAGTESRFKQWTMMEGLPSVASQDANMHKNGAIVPPGKARGGSPYNQFDIIQGDPLGGHAGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFSDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTGLPRPPPGLTNPKPSSPWNSTAPRSVRGWGAQDSRLASGEKDHLITPLIRPEECAFYSTNGLEERKLQLPASTWSDGSSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYNTKQEAAKAQTALHMCVLGNTTILAEFATDEEVSRFLTQAQPPTPAATPSAPTAGWQSLETGQNQTDPVGPALNLFGGSTGLGQWSSGGGGSSGGDLTGASLWGPPNYSSSLWGVPSVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_004937725.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004937725.3

ORF Insert Sequence:

ATGAGAGAGA AGGAGCAAGA AAGGGAAGAA CAGTTAATGG AAGACAAGAA AAGGAAGAAG 
GAGGATAAGA AAAAGAAGGA ATCTGCTCAG AAGGTCACAG ATCAAAAAAC CAAAGTGCCC 
GAAGTGACGA AACCAAGTTT AAGCCAACCA GTGGCTGCCA GCCCAATTGG CAACTCTCCA 
TCGCCACCAG TCAATGGTGG CAACAATGCC AAAAGGGTGG CAGTGCCGAA CGGACAACCG 
CCAAGCGCCG CCCGCTACAT GCCTCGGGAG GTGCCGCCGC GATTCCGTTG CCAGCAGGAC 
CACAAAGTGT TACTGAAACG TGGGCAGCCC CCTCCACCAT CCTGTATGCT CCTTGGGGGT 
GGAGCAGGGC CTCCTCCCCC TACAGCACCA GGAGCAAACC CAAACAACGC ACAACCAGTG 
ACAGGAGCAC TGCTGCAGAG CGACAGTGGG ACTGCAACAG ATTCAACAAT TGGAGGTGCT 
GCTGCTTCAA ATTATGCAAA TTCCACTTGG GGTCCTGGAG CCTCCTCCAG CAGCGGCACC 
AACGCCAACC CAACTCACAT CTGGGACAAG GTGATTGTAG ACGGGTCTGA CATGGAAGAG 
TGGCCTTGTA TCGCCAGCAA AGATGCTGAG TCTTCTTCCG AAAACACCAC CGATAACAAC 
AGTGCCTCGA ACCCTGGCTT GGAGAAGAAC ACTCTGCCAG GAAGCACCAC TAGTAACAAA 
GGAAAAGGAA GCCAGTGCCA GTCTGGAAGC GCTGGAAATG AATGTAATCT TGGGGCCTGG 
AAATCTGATC CCAAGGCTAA ATCTGTTCAA TCTTCCAACC CTGCTACCGA GAGTAACAGT 
GGACTAAGTA ATTGGAGGAA CTTGACTGGA CAAGATAGAA TTGGTTCTGG TTCTGGCTTC 
AGCAACTTTA ACCCAAATAG CAACCCGTCT GCTTGGCCAG CACTGGTCCA AGAGGGCAAT 
TCTAGGAAAG GGACTTCGGA ATCTGATAGT GGTAGCTCCA ATGCACAGAT TAGCACAGTA 
GGTCAGACCT CTAGGGAACA GCAGTCAAAG ATGGAAAATG CGGGTGTTAA CTTTGTCTCT 
GGCAGAGAAC AGGCTCAAAT TCATAACACT GATGGACCAA AAAATGGAAA CACTAACTCC 
TTGAACTTAA GTTCACCAAA CCCTATGGAG AATAAGGGAA TGCCCTTTGA AATGGGCTTG 
GGGAACCCTT CCAGGAGCAC TGACACCCCT TCACAAAGCA CTGGAGAAAG AAAGACTGGG 
AGTGTTGGAT CTTGGGGTAC AGCTAGGGGG TCTTCTGGAA CTGACACAGT CTCTGGACAG 
AGCAATTCTG GAAACCATGG GAACAATGGA AAGGATAGGG AGGACTCCTG GAAAGGAGCT 
TCTGTTCAAA AACCTAATGG GTCAAGAAGC GATTCTTGGG ATAACAATAA CCGGTCTACG 
GGTGGTGGGT CTTGGAACTT TGGCCCTCAG CATGCAAATG AAAGCAAATG GGGTGAAGGG 
AACAAATTAA CATCTGGGGT CTCTCAGGGA GAATGGAAAC AGCTGTCTGG GTCTGATGAA 
CTGAAGATTG GAGAATGGAG TGGTCCAAAC CAACCAAATT CTAGCACTGG AGCATGGGAC 
AATCAAAAAG GCCACCCTCT TCCTGAAAAC CAAGGCAATT CCCAGGCTCC CTGTTGGGGA 
AGATCTTCCA GCTCTGCAGG AAGTGAGGTT GGAGGTCAAA GCACTGGAAG CAACCACAAA 
GCAGGAAGTA GTGACAGTCA CAATTCTGGA CGCCGATCAT ACAGGCCTAC GCATCCTGAT 
TGCCAGGCAG TCTTGCAGAC TTTGCTGAGC AGGACTGATT TGGACCCCCG AGTGCTTTCA 
AACACTGGCT GGGGCCAAAC TCAAATTAAG CAAGATACCG TATGGGATAT TGAAGAGATG 
CCACGGTCCG AGGGGAAGTC TGATAAAGGA ACTGAGGGGT GGGAGAGCTC TGCCACACAG 
ACAAAGAACT CAGGGGGCTG GGGCGATGTA CCCAGCCAAA GCAATCAAAT CAAGTCTGGA 
TGGGGTGAGC TCTCAACCCC AACAGAATGG AAGGACCCCA AAAATACTGG AGGATGGAAT 
GACTATAAGA ACCCCAATTC GTCTAATTGG GGAGGGGGAA GACAAGAAGA GAAATCGTCA 
TCTTCTTGGA ATGACAGCTC TAGTAAGGAT CAGGGGTGGG GTGGACGGCA GCCTAATCAA 
GGATGGTCTT CTGGAAAGAA TGGTTGGGGA GAGGAAGTTG ACCAAGCAAA AAACAGCAAC 
TGGGAAAGTG CGGGAAATAA GCCAGCATCT GGTTGGGGTG AAGGTGGGCA AAATGAGATT 
GGAACTTGGG GTAACGGTGC AAATACAGGC TCTGCTTCAA AGGGTGGTTG GGATGATTGT 
AAAAGAAATT CAACGTGGAA CGAGACGGGT CGACAGCCCA ACTCCTGGAA CAAGCAGCAG 
CAGCAGCAAC AGCAGCAACA GCAAGAGACT TCTGGTTCCT GGGGTCCACC GCCACAAACT 
CCAAGTAATG GGCGACCTCC AAACCCAAAC TGGAACAGCG GACCACAACC AGCTGCCCCC 
AAAGACGAGG AGCCTAGTGG CTGGGAAGAA CCTTCTCCAC AGTCAATCAG TCGAAAAATG 
GATATTGATG ATGGCACTTC AGCGTGGGGA GATCCTAGCA GTTATAACTA CAAGAATGTG 
AACCTGTGGG ACAAGAACTC ACAAGGGGGA CAGGCCCCAC GGGAACAGAG CCTGCCTACT 
CCAATGACTA GCAAATCACA AGCATCAGTC TGGAGCAAAA GCACTCCACC TGCTCCAGAT 
AATGGTACGT CCGCCTGGGG TGAGCCAAAT GAAACCAGTC CCGGGTGGGG TGAAGTAGAT 
GATACAGGAG CATCGACAAC AGGCTGGGGG AATGCACCTT CCAACGCCCC GAATGCCATG 
AAATCTAGTT CTAAATCTAT GCAAGATGGC TGGGGAGAGA GTGATGGGCC AGTGACAGGA 
ACACGTCATT CCAGCTGGGA AGAGGAGGAG GAGGGAGGAG TCTGGAACAC AGCAGGCTCT 
CAGGGAAGCA GTTCCTCCCA CAACTCAACA AGCTGGGGGC AAGGAGGAAA GAAAACGCAG 
ATGAAGTGCT CGTTAAAAGG AGGAAATAGT GATTCATGGA TGAACCCTAT ATCCAAGCAG 
TTTTCAAATA TGGGCTTGCT AAGTCAAACT GAGGACATTC CAGGCAATAA GATGGACTTG 
TCTGTAGGTG GTCTCCCAGA TAAGAAGTTT GAGGTAGATA AACGAGCCAT GAATCTCGGG 
GATTTTAATG ACATAATGAG AAAGGATCGA TCTGGGTTCC GTCCACCTAA TTCCAAAGAC 
ATGGGAACCA CAGATAGTGG GCCTTATTTT GAGAAGCTGA CTTTGCCTTT CTCCAATCAA 
GATGGGTGCC TTGGGGATGA GGCTCCCTGC TCTCCCTTCT CCCCTTCTCC CAGCTACAAG 
CTGTCTCCCT CTGGTTCCAC ACTATCCAAC GTCGGCCTTG GAGCAATCGG CTCAGGGCTC 
AGTCCCCAAA ACTACGCTGC TAGACAAGGT GGCAATCATG GTTTGTTTGG AAACAGCACA 
GCACAATCGA GGGGCATGCA CACACCAGTG CAGCCACTAA ATCCTTCCCC CAATATCCGG 
GCGCAAGTGC CTCCCCAATT TCTTTCTCCC CAGGTTTCGG CCTCCATGCT CAAACAGTTC 
CCCAACAGTG GCTTGAATCC AGGTCTTTTC AATGTGGGGC CCCAGCTTTC TCCACAACAG 
ATTGCCATGC TGAGCCAGCT TCCACAGATT CCCCAGTTTC AATTAGCATG TCAGCTCCTC 
CTTCAGCAGC AGCAGCAGCA GCAGCAGCTG TTACAGAGCC AGAGGAAGTT ATCTCAAGCT 
GTGCGACAGC AGCAGGAGCA ACAGATTGCT CGTATGGTGA GTGCCCTCCA GCAGCAGCAG 
CAGAGGCAGC CTGGCATGAA GCATTCACCA TCCCACCCTG TTGGGCCTAA GCCACATATA 
GACAGCATGG TACCCAATCC TTTGAATGTG GGTCTTTCAG ATTTACAGAC CAAAGGACAA 
ATACCTGGAT ACGGTTCAGG CTTTGGCTCA AGCAGCATGG ATTATGGCAT GGTGGGAGGG 
AAAGAAGCTG GAACAGAATC CCGCTTTAAA CAGTGGACTA TGATGGAAGG GCTGCCCTCT 
GTGGCTTCAC AAGATGCCAA TATGCACAAA AATGGTGCAA TAGTGCCCCC TGGAAAGGCT 
CGCGGAGGAT CGCCCTACAA CCAGTTCGAC ATAATTCAGG GCGACCCACT AGGTGGCCAT 
GCAGGCCCTG CTGGTGATAG TTGGTTACCT GCCAAATCTC CACCGACAAA CAAGATCGGA 
AGCAAATCCA GCAACGCCAG CTGGCCTCCA GAGTTCCAAC CAGGAGTGCC ATGGAAAGGT 
ATACAAAACA TTGACCCTGA ATCTGACCCC TACGTGACCC CTGGAAGTGT GCTGGGGGGG 
ACAGCCACAT CTCCCATTGT AGATACTGAC CACCAACTTC TGCGGGACAA CACCACAGGG 
TCTAATTCTT CCCTCAACAC CTCGCTGCCT TCACCTGGTG CCTGGCCCTA CAGTGCCTCT 
GACAATTCCT TCACCAACGT TCATAGCACT TCAGCAAAAT TCAGTGATTA CAAATCAACA 
TGGTCCCCAG ATCCCATAGG ACACAATCCC ACTCATCTCT CCAACAAGAT GTGGAAAAAC 
CATATTTCCT CAAGGAACAC TACAGGGCTG CCTCGCCCGC CTCCTGGCCT GACCAACCCC 
AAACCATCAT CTCCATGGAA CAGCACAGCA CCCCGATCGG TCAGGGGCTG GGGGGCACAG 
GACTCAAGGC TTGCCTCTGG TGAGAAGGAT CATCTGATAA CGCCATTGAT CAGACCTGAG 
GAGTGTGCAT TTTACAGCAC TAATGGTTTG GAGGAGAGAA AGTTGCAGTT GCCTGCATCT 
ACTTGGAGTG ATGGTAGCTC AGTTCGTCCT AGCTACTGGC TGGTTCTTCA CAATCTCACT 
CCACAGATCG ATGGGTCAAC CTTGAGGACG ATCTGCATGC AGCATGGTCC ACTGCTGACA 
TTCCATCTGA ACCTGACCCA GGGCACCGCT CTCATCCGAT ACAACACCAA ACAGGAGGCG 
GCCAAGGCCC AGACTGCACT GCACATGTGT GTGTTGGGAA ACACTACCAT CCTTGCTGAG 
TTTGCCACAG ATGAAGAGGT TAGTCGCTTT TTGACACAAG CTCAGCCCCC TACACCTGCA 
GCAACCCCAA GTGCACCCAC GGCAGGCTGG CAATCCCTGG AGACAGGACA GAACCAGACA 
GATCCAGTTG GACCTGCTTT GAATCTCTTT GGTGGGTCAA CAGGGCTTGG GCAGTGGAGC 
AGTGGTGGCG GCGGCAGCAG TGGGGGTGAT CTCACTGGCG CTTCGTTGTG GGGGCCCCCA 
AACTATTCTT CAAGCTTGTG GGGGGTCCCA AGCGTAGAGG ATCCACACAG AATGGGCAGC 
CCTGCTCCCT TACTACCTGG AGACCTTCTG GGAGGAGGGT CGGATTCAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_025155203.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025010971.1 Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X6, mRNA.

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CloneID	OGa01774
Clone ID Related Accession (Same CDS sequence)	XM_025155203.1
Accession Version	XM_025155203.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	trinucleotide repeat-containing gene 6B protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGA GAAGGGGAAA AAAAGTGCCC GAAGTGACGA AACCAAGTTT AAGCCAACCA 
GTGGCTGCCA GCCCAATTGG CAACTCTCCA TCGCCACCAG TCAATGGTGG CAACAATGCC 
AAAAGGGTGG CAGTGCCGAA CGGACAACCG CCAAGCGCCG CCCGCTACAT GCCTCGGGAG 
GTGCCGCCGC GATTCCGTTG CCAGCAGGAC CACAAAGTGT TACTGAAACG TGGGCAGCCC 
CCTCCACCAT CCTGTATGCT CCTTGGGGGT GGAGCAGGGC CTCCTCCCCC TACAGCACCA 
GGAGCAAACC CAAACAACGC ACAACCAGTG ACAGGAGCAC TGCTGCAGAG CGACAGTGGG 
ACTGCAACAG ATTCAACAAT TGGAGGTGCT GCTGCTTCAA ATTATGCAAA TTCCACTTGG 
GGTCCTGGAG CCTCCTCCAG CAGCGGCACC AACGCCAACC CAACTCACAT CTGGGACAAG 
GTGATTGTAG ACGGGTCTGA CATGGAAGAG TGGCCTTGTA TCGCCAGCAA AGATGCTGAG 
TCTTCTTCCG AAAACACCAC CGATAACAAC AGTGCCTCGA ACCCTGGCTT GGAGAAGAAC 
ACTCTGCCAG GAAGCACCAC TAGTAACAAA GGAAAAGGAA GCCAGTGCCA GTCTGGAAGC 
GCTGGAAATG AATGTAATCT TGGGGCCTGG AAATCTGATC CCAAGGCTAA ATCTGTTCAA 
TCTTCCAACC CTGCTACCGA GAGTAACAGT GGACTAAGTA ATTGGAGGAA CTTGACTGGA 
CAAGATAGAA TTGGTTCTGG TTCTGGCTTC AGCAACTTTA ACCCAAATAG CAACCCGTCT 
GCTTGGCCAG CACTGGTCCA AGAGGGCAAT TCTAGGAAAG GGACTTCGGA ATCTGATAGT 
GGTAGCTCCA ATGCACAGAT TAGCACAGTA GGTCAGACCT CTAGGGAACA GCAGTCAAAG 
ATGGAAAATG CGGGTGTTAA CTTTGTCTCT GGCAGAGAAC AGGCTCAAAT TCATAACACT 
GATGGACCAA AAAATGGAAA CACTAACTCC TTGAACTTAA GTTCACCAAA CCCTATGGAG 
AATAAGGGAA TGCCCTTTGA AATGGGCTTG GGGAACCCTT CCAGGAGCAC TGACACCCCT 
TCACAAAGCA CTGGAGAAAG AAAGACTGGG AGTGTTGGAT CTTGGGGTAC AGCTAGGGGG 
TCTTCTGGAA CTGACACAGT CTCTGGACAG AGCAATTCTG GAAACCATGG GAACAATGGA 
AAGGATAGGG AGGACTCCTG GAAAGGAGCT TCTGTTCAAA AACCTAATGG GTCAAGAAGC 
GATTCTTGGG ATAACAATAA CCGGTCTACG GGTGGTGGGT CTTGGAACTT TGGCCCTCAG 
CATGCAAATG AAAGCAAATG GGGTGAAGGG AACAAATTAA CATCTGGGGT CTCTCAGGGA 
GAATGGAAAC AGCTGTCTGG GTCTGATGAA CTGAAGATTG GAGAATGGAG TGGTCCAAAC 
CAACCAAATT CTAGCACTGG AGCATGGGAC AATCAAAAAG GCCACCCTCT TCCTGAAAAC 
CAAGGCAATT CCCAGGCTCC CTGTTGGGGA AGATCTTCCA GCTCTGCAGG AAGTGAGGTT 
GGAGGTCAAA GCACTGGAAG CAACCACAAA GCAGGAAGTA GTGACAGTCA CAATTCTGGA 
CGCCGATCAT ACAGGCCTAC GCATCCTGAT TGCCAGGCAG TCTTGCAGAC TTTGCTGAGC 
AGGACTGATT TGGACCCCCG AGTGCTTTCA AACACTGGCT GGGGCCAAAC TCAAATTAAG 
CAAGATACCG TATGGGATAT TGAAGAGATG CCACGGTCCG AGGGGAAGTC TGATAAAGGA 
ACTGAGGGGT GGGAGAGCTC TGCCACACAG ACAAAGAACT CAGGGGGCTG GGGCGATGTA 
CCCAGCCAAA GCAATCAAAT CAAGTCTGGA TGGGGTGAGC TCTCAACCCC AACAGAATGG 
AAGGACCCCA AAAATACTGG AGGATGGAAT GACTATAAGA ACCCCAATTC GTCTAATTGG 
GGAGGGGGAA GACAAGAAGA GAAATCGTCA TCTTCTTGGA ATGACAGCTC TAGTAAGGAT 
CAGGGGTGGG GTGGACGGCA GCCTAATCAA GGATGGTCTT CTGGAAAGAA TGGTTGGGGA 
GAGGAAGTTG ACCAAGCAAA AAACAGCAAC TGGGAAAGTG CGGGAAATAA GCCAGCATCT 
GGTTGGGGTG AAGGTGGGCA AAATGAGATT GGAACTTGGG GTAACGGTGC AAATACAGGC 
TCTGCTTCAA AGGGTGGTTG GGATGATTGT AAAAGAAATT CAACGTGGAA CGAGACGGGT 
CGACAGCCCA ACTCCTGGAA CAAGCAGCAG CAGCAGCAAC AGCAGCAACA GCAAGAGACT 
TCTGGTTCCT GGGGTCCACC GCCACAAACT CCAAGTAATG GGCGACCTCC AAACCCAAAC 
TGGAACAGCG GACCACAACC AGCTGCCCCC AAAGACGAGG AGCCTAGTGG CTGGGAAGAA 
CCTTCTCCAC AGTCAATCAG TCGAAAAATG GATATTGATG ATGGCACTTC AGCGTGGGGA 
GATCCTAGCA GTTATAACTA CAAGAATGTG AACCTGTGGG ACAAGAACTC ACAAGGGGGA 
CAGGCCCCAC GGGAACAGAG CCTGCCTACT CCAATGACTA GCAAATCACA AGCATCAGTC 
TGGAGCAAAA GCACTCCACC TGCTCCAGAT AATGGTACGT CCGCCTGGGG TGAGCCAAAT 
GAAACCAGTC CCGGGTGGGG TGAAGTAGAT GATACAGGAG CATCGACAAC AGGCTGGGGG 
AATGCACCTT CCAACGCCCC GAATGCCATG AAATCTAGTT CTAAATCTAT GCAAGATGGC 
TGGGGAGAGA GTGATGGGCC AGTGACAGGA ACACGTCATT CCAGCTGGGA AGAGGAGGAG 
GAGGGAGGAG TCTGGAACAC AGCAGGCTCT CAGGGAAGCA GTTCCTCCCA CAACTCAACA 
AGCTGGGGGC AAGGAGGAAA GAAAACGCAG ATGAAGTGCT CGTTAAAAGG AGGAAATAGT 
GATTCATGGA TGAACCCTAT ATCCAAGCAG TTTTCAAATA TGGGCTTGCT AAGTCAAACT 
GAGGACATTC CAGGCAATAA GATGGACTTG TCTGTAGGTG GTCTCCCAGA TAAGAAGTTT 
GAGGTAGATA AACGAGCCAT GAATCTCGGG GATTTTAATG ACATAATGAG AAAGGATCGA 
TCTGGGTTCC GTCCACCTAA TTCCAAAGAC ATGGGAACCA CAGATAGTGG GCCTTATTTT 
GAGAAGCTGA CTTTGCCTTT CTCCAATCAA GATGGGTGCC TTGGGGATGA GGCTCCCTGC 
TCTCCCTTCT CCCCTTCTCC CAGCTACAAG CTGTCTCCCT CTGGTTCCAC ACTATCCAAC 
GTCGGCCTTG GAGCAATCGG CTCAGGGCTC AGTCCCCAAA ACTACGCTGC TAGACAAGGT 
GGCAATCATG GTTTGTTTGG AAACAGCACA GCACAATCGA GGGGCATGCA CACACCAGTG 
CAGCCACTAA ATCCTTCCCC CAATATCCGG GCGCAAGTGC CTCCCCAATT TCTTTCTCCC 
CAGGTTTCGG CCTCCATGCT CAAACAGTTC CCCAACAGTG GCTTGAATCC AGGTCTTTTC 
AATGTGGGGC CCCAGCTTTC TCCACAACAG ATTGCCATGC TGAGCCAGCT TCCACAGATT 
CCCCAGTTTC AATTAGCATG TCAGCTCCTC CTTCAGCAGC AGCAGCAGCA GCAGCAGCTG 
TTACAGAGCC AGAGGAAGTT ATCTCAAGCT GTGCGACAGC AGCAGGAGCA ACAGATTGCT 
CGTATGGTGA GTGCCCTCCA GCAGCAGCAG CAGAGGCAGC CTGGCATGAA GCATTCACCA 
TCCCACCCTG TTGGGCCTAA GCCACATATA GACAGCATGG TACCCAATCC TTTGAATGTG 
GGTCTTTCAG ATTTACAGAC CAAAGGACAA ATACCTGGAT ACGGTTCAGG CTTTGGCTCA 
AGCAGCATGG ATTATGGCAT GGTGGGAGGG AAAGAAGCTG GAACAGAATC CCGCTTTAAA 
CAGTGGACTA TGATGGAAGG GCTGCCCTCT GTGGCTTCAC AAGATGCCAA TATGCACAAA 
AATGGTGCAA TAGTGCCCCC TGGAAAGGCT CGCGGAGGAT CGCCCTACAA CCAGTTCGAC 
ATAATTCAGG GCGACCCACT AGGTGGCCAT GCAGGCCCTG CTGGTGATAG TTGGTTACCT 
GCCAAATCTC CACCGACAAA CAAGATCGGA AGCAAATCCA GCAACGCCAG CTGGCCTCCA 
GAGTTCCAAC CAGGAGTGCC ATGGAAAGGT ATACAAAACA TTGACCCTGA ATCTGACCCC 
TACGTGACCC CTGGAAGTGT GCTGGGGGGG ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGACAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAATTCCT TCACCAACGT TCATAGCACT 
TCAGCAAAAT TCAGTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAATCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACAGGGCTG 
CCTCGCCCGC CTCCTGGCCT GACCAACCCC AAACCATCAT CTCCATGGAA CAGCACAGCA 
CCCCGATCGG TCAGGGGCTG GGGGGCACAG GACTCAAGGC TTGCCTCTGG TGAGAAGGAT 
CATCTGATAA CGCCATTGAT CAGACCTGAG GAGTGTGCAT TTTACAGCAC TAATGGTTTG 
GAGGAGAGAA AGTTGCAGTT GCCTGCATCT ACTTGGAGTG ATGGTAGCTC AGTTCGTCCT 
AGCTACTGGC TGGTTCTTCA CAATCTCACT CCACAGATCG ATGGGTCAAC CTTGAGGACG 
ATCTGCATGC AGCATGGTCC ACTGCTGACA TTCCATCTGA ACCTGACCCA GGGCACCGCT 
CTCATCCGAT ACAACACCAA ACAGGAGGCG GCCAAGGCCC AGACTGCACT GCACATGTGT 
GTGTTGGGAA ACACTACCAT CCTTGCTGAG TTTGCCACAG ATGAAGAGGT TAGTCGCTTT 
TTGACACAAG CTCAGCCCCC TACACCTGCA GCAACCCCAA GTGCACCCAC GGCAGGCTGG 
CAATCCCTGG AGACAGGACA GAACCAGACA GATCCAGTTG GACCTGCTTT GAATCTCTTT 
GGTGGGTCAA CAGGGCTTGG GCAGTGGAGC AGTGGTGGCG GCGGCAGCAG TGGGGGTGAT 
CTCACTGGCG CTTCGTTGTG GGGGCCCCCA AACTATTCTT CAAGCTTGTG GGGGGTCCCA 
AGCGTAGAGG ATCCACACAG AATGGGCAGC CCTGCTCCCT TACTACCTGG AGACCTTCTG 
GGAGGAGGGT CGGATTCAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025010971.1
CDS	39..5522
Translation	MGKRRGKKVPEVTKPSLSQPVAASPIGNSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPPTAPGANPNNAQPVTGALLQSDSGTATDSTIGGAAASNYANSTWGPGASSSSGTNANPTHIWDKVIVDGSDMEEWPCIASKDAESSSENTTDNNSASNPGLEKNTLPGSTTSNKGKGSQCQSGSAGNECNLGAWKSDPKAKSVQSSNPATESNSGLSNWRNLTGQDRIGSGSGFSNFNPNSNPSAWPALVQEGNSRKGTSESDSGSSNAQISTVGQTSREQQSKMENAGVNFVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFEMGLGNPSRSTDTPSQSTGERKTGSVGSWGTARGSSGTDTVSGQSNSGNHGNNGKDREDSWKGASVQKPNGSRSDSWDNNNRSTGGGSWNFGPQHANESKWGEGNKLTSGVSQGEWKQLSGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNSQAPCWGRSSSSAGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEMPRSEGKSDKGTEGWESSATQTKNSGGWGDVPSQSNQIKSGWGELSTPTEWKDPKNTGGWNDYKNPNSSNWGGGRQEEKSSSSWNDSSSKDQGWGGRQPNQGWSSGKNGWGEEVDQAKNSNWESAGNKPASGWGEGGQNEIGTWGNGANTGSASKGGWDDCKRNSTWNETGRQPNSWNKQQQQQQQQQQETSGSWGPPPQTPSNGRPPNPNWNSGPQPAAPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPSSYNYKNVNLWDKNSQGGQAPREQSLPTPMTSKSQASVWSKSTPPAPDNGTSAWGEPNETSPGWGEVDDTGASTTGWGNAPSNAPNAMKSSSKSMQDGWGESDGPVTGTRHSSWEEEEEGGVWNTAGSQGSSSSHNSTSWGQGGKKTQMKCSLKGGNSDSWMNPISKQFSNMGLLSQTEDIPGNKMDLSVGGLPDKKFEVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLSNVGLGAIGSGLSPQNYAARQGGNHGLFGNSTAQSRGMHTPVQPLNPSPNIRAQVPPQFLSPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQQLLQSQRKLSQAVRQQQEQQIARMVSALQQQQQRQPGMKHSPSHPVGPKPHIDSMVPNPLNVGLSDLQTKGQIPGYGSGFGSSSMDYGMVGGKEAGTESRFKQWTMMEGLPSVASQDANMHKNGAIVPPGKARGGSPYNQFDIIQGDPLGGHAGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFSDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTGLPRPPPGLTNPKPSSPWNSTAPRSVRGWGAQDSRLASGEKDHLITPLIRPEECAFYSTNGLEERKLQLPASTWSDGSSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYNTKQEAAKAQTALHMCVLGNTTILAEFATDEEVSRFLTQAQPPTPAATPSAPTAGWQSLETGQNQTDPVGPALNLFGGSTGLGQWSSGGGGSSGGDLTGASLWGPPNYSSSLWGVPSVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_025155203.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025155203.1

ORF Insert Sequence:

ATGGGGAAGA GAAGGGGAAA AAAAGTGCCC GAAGTGACGA AACCAAGTTT AAGCCAACCA 
GTGGCTGCCA GCCCAATTGG CAACTCTCCA TCGCCACCAG TCAATGGTGG CAACAATGCC 
AAAAGGGTGG CAGTGCCGAA CGGACAACCG CCAAGCGCCG CCCGCTACAT GCCTCGGGAG 
GTGCCGCCGC GATTCCGTTG CCAGCAGGAC CACAAAGTGT TACTGAAACG TGGGCAGCCC 
CCTCCACCAT CCTGTATGCT CCTTGGGGGT GGAGCAGGGC CTCCTCCCCC TACAGCACCA 
GGAGCAAACC CAAACAACGC ACAACCAGTG ACAGGAGCAC TGCTGCAGAG CGACAGTGGG 
ACTGCAACAG ATTCAACAAT TGGAGGTGCT GCTGCTTCAA ATTATGCAAA TTCCACTTGG 
GGTCCTGGAG CCTCCTCCAG CAGCGGCACC AACGCCAACC CAACTCACAT CTGGGACAAG 
GTGATTGTAG ACGGGTCTGA CATGGAAGAG TGGCCTTGTA TCGCCAGCAA AGATGCTGAG 
TCTTCTTCCG AAAACACCAC CGATAACAAC AGTGCCTCGA ACCCTGGCTT GGAGAAGAAC 
ACTCTGCCAG GAAGCACCAC TAGTAACAAA GGAAAAGGAA GCCAGTGCCA GTCTGGAAGC 
GCTGGAAATG AATGTAATCT TGGGGCCTGG AAATCTGATC CCAAGGCTAA ATCTGTTCAA 
TCTTCCAACC CTGCTACCGA GAGTAACAGT GGACTAAGTA ATTGGAGGAA CTTGACTGGA 
CAAGATAGAA TTGGTTCTGG TTCTGGCTTC AGCAACTTTA ACCCAAATAG CAACCCGTCT 
GCTTGGCCAG CACTGGTCCA AGAGGGCAAT TCTAGGAAAG GGACTTCGGA ATCTGATAGT 
GGTAGCTCCA ATGCACAGAT TAGCACAGTA GGTCAGACCT CTAGGGAACA GCAGTCAAAG 
ATGGAAAATG CGGGTGTTAA CTTTGTCTCT GGCAGAGAAC AGGCTCAAAT TCATAACACT 
GATGGACCAA AAAATGGAAA CACTAACTCC TTGAACTTAA GTTCACCAAA CCCTATGGAG 
AATAAGGGAA TGCCCTTTGA AATGGGCTTG GGGAACCCTT CCAGGAGCAC TGACACCCCT 
TCACAAAGCA CTGGAGAAAG AAAGACTGGG AGTGTTGGAT CTTGGGGTAC AGCTAGGGGG 
TCTTCTGGAA CTGACACAGT CTCTGGACAG AGCAATTCTG GAAACCATGG GAACAATGGA 
AAGGATAGGG AGGACTCCTG GAAAGGAGCT TCTGTTCAAA AACCTAATGG GTCAAGAAGC 
GATTCTTGGG ATAACAATAA CCGGTCTACG GGTGGTGGGT CTTGGAACTT TGGCCCTCAG 
CATGCAAATG AAAGCAAATG GGGTGAAGGG AACAAATTAA CATCTGGGGT CTCTCAGGGA 
GAATGGAAAC AGCTGTCTGG GTCTGATGAA CTGAAGATTG GAGAATGGAG TGGTCCAAAC 
CAACCAAATT CTAGCACTGG AGCATGGGAC AATCAAAAAG GCCACCCTCT TCCTGAAAAC 
CAAGGCAATT CCCAGGCTCC CTGTTGGGGA AGATCTTCCA GCTCTGCAGG AAGTGAGGTT 
GGAGGTCAAA GCACTGGAAG CAACCACAAA GCAGGAAGTA GTGACAGTCA CAATTCTGGA 
CGCCGATCAT ACAGGCCTAC GCATCCTGAT TGCCAGGCAG TCTTGCAGAC TTTGCTGAGC 
AGGACTGATT TGGACCCCCG AGTGCTTTCA AACACTGGCT GGGGCCAAAC TCAAATTAAG 
CAAGATACCG TATGGGATAT TGAAGAGATG CCACGGTCCG AGGGGAAGTC TGATAAAGGA 
ACTGAGGGGT GGGAGAGCTC TGCCACACAG ACAAAGAACT CAGGGGGCTG GGGCGATGTA 
CCCAGCCAAA GCAATCAAAT CAAGTCTGGA TGGGGTGAGC TCTCAACCCC AACAGAATGG 
AAGGACCCCA AAAATACTGG AGGATGGAAT GACTATAAGA ACCCCAATTC GTCTAATTGG 
GGAGGGGGAA GACAAGAAGA GAAATCGTCA TCTTCTTGGA ATGACAGCTC TAGTAAGGAT 
CAGGGGTGGG GTGGACGGCA GCCTAATCAA GGATGGTCTT CTGGAAAGAA TGGTTGGGGA 
GAGGAAGTTG ACCAAGCAAA AAACAGCAAC TGGGAAAGTG CGGGAAATAA GCCAGCATCT 
GGTTGGGGTG AAGGTGGGCA AAATGAGATT GGAACTTGGG GTAACGGTGC AAATACAGGC 
TCTGCTTCAA AGGGTGGTTG GGATGATTGT AAAAGAAATT CAACGTGGAA CGAGACGGGT 
CGACAGCCCA ACTCCTGGAA CAAGCAGCAG CAGCAGCAAC AGCAGCAACA GCAAGAGACT 
TCTGGTTCCT GGGGTCCACC GCCACAAACT CCAAGTAATG GGCGACCTCC AAACCCAAAC 
TGGAACAGCG GACCACAACC AGCTGCCCCC AAAGACGAGG AGCCTAGTGG CTGGGAAGAA 
CCTTCTCCAC AGTCAATCAG TCGAAAAATG GATATTGATG ATGGCACTTC AGCGTGGGGA 
GATCCTAGCA GTTATAACTA CAAGAATGTG AACCTGTGGG ACAAGAACTC ACAAGGGGGA 
CAGGCCCCAC GGGAACAGAG CCTGCCTACT CCAATGACTA GCAAATCACA AGCATCAGTC 
TGGAGCAAAA GCACTCCACC TGCTCCAGAT AATGGTACGT CCGCCTGGGG TGAGCCAAAT 
GAAACCAGTC CCGGGTGGGG TGAAGTAGAT GATACAGGAG CATCGACAAC AGGCTGGGGG 
AATGCACCTT CCAACGCCCC GAATGCCATG AAATCTAGTT CTAAATCTAT GCAAGATGGC 
TGGGGAGAGA GTGATGGGCC AGTGACAGGA ACACGTCATT CCAGCTGGGA AGAGGAGGAG 
GAGGGAGGAG TCTGGAACAC AGCAGGCTCT CAGGGAAGCA GTTCCTCCCA CAACTCAACA 
AGCTGGGGGC AAGGAGGAAA GAAAACGCAG ATGAAGTGCT CGTTAAAAGG AGGAAATAGT 
GATTCATGGA TGAACCCTAT ATCCAAGCAG TTTTCAAATA TGGGCTTGCT AAGTCAAACT 
GAGGACATTC CAGGCAATAA GATGGACTTG TCTGTAGGTG GTCTCCCAGA TAAGAAGTTT 
GAGGTAGATA AACGAGCCAT GAATCTCGGG GATTTTAATG ACATAATGAG AAAGGATCGA 
TCTGGGTTCC GTCCACCTAA TTCCAAAGAC ATGGGAACCA CAGATAGTGG GCCTTATTTT 
GAGAAGCTGA CTTTGCCTTT CTCCAATCAA GATGGGTGCC TTGGGGATGA GGCTCCCTGC 
TCTCCCTTCT CCCCTTCTCC CAGCTACAAG CTGTCTCCCT CTGGTTCCAC ACTATCCAAC 
GTCGGCCTTG GAGCAATCGG CTCAGGGCTC AGTCCCCAAA ACTACGCTGC TAGACAAGGT 
GGCAATCATG GTTTGTTTGG AAACAGCACA GCACAATCGA GGGGCATGCA CACACCAGTG 
CAGCCACTAA ATCCTTCCCC CAATATCCGG GCGCAAGTGC CTCCCCAATT TCTTTCTCCC 
CAGGTTTCGG CCTCCATGCT CAAACAGTTC CCCAACAGTG GCTTGAATCC AGGTCTTTTC 
AATGTGGGGC CCCAGCTTTC TCCACAACAG ATTGCCATGC TGAGCCAGCT TCCACAGATT 
CCCCAGTTTC AATTAGCATG TCAGCTCCTC CTTCAGCAGC AGCAGCAGCA GCAGCAGCTG 
TTACAGAGCC AGAGGAAGTT ATCTCAAGCT GTGCGACAGC AGCAGGAGCA ACAGATTGCT 
CGTATGGTGA GTGCCCTCCA GCAGCAGCAG CAGAGGCAGC CTGGCATGAA GCATTCACCA 
TCCCACCCTG TTGGGCCTAA GCCACATATA GACAGCATGG TACCCAATCC TTTGAATGTG 
GGTCTTTCAG ATTTACAGAC CAAAGGACAA ATACCTGGAT ACGGTTCAGG CTTTGGCTCA 
AGCAGCATGG ATTATGGCAT GGTGGGAGGG AAAGAAGCTG GAACAGAATC CCGCTTTAAA 
CAGTGGACTA TGATGGAAGG GCTGCCCTCT GTGGCTTCAC AAGATGCCAA TATGCACAAA 
AATGGTGCAA TAGTGCCCCC TGGAAAGGCT CGCGGAGGAT CGCCCTACAA CCAGTTCGAC 
ATAATTCAGG GCGACCCACT AGGTGGCCAT GCAGGCCCTG CTGGTGATAG TTGGTTACCT 
GCCAAATCTC CACCGACAAA CAAGATCGGA AGCAAATCCA GCAACGCCAG CTGGCCTCCA 
GAGTTCCAAC CAGGAGTGCC ATGGAAAGGT ATACAAAACA TTGACCCTGA ATCTGACCCC 
TACGTGACCC CTGGAAGTGT GCTGGGGGGG ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGACAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAATTCCT TCACCAACGT TCATAGCACT 
TCAGCAAAAT TCAGTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAATCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACAGGGCTG 
CCTCGCCCGC CTCCTGGCCT GACCAACCCC AAACCATCAT CTCCATGGAA CAGCACAGCA 
CCCCGATCGG TCAGGGGCTG GGGGGCACAG GACTCAAGGC TTGCCTCTGG TGAGAAGGAT 
CATCTGATAA CGCCATTGAT CAGACCTGAG GAGTGTGCAT TTTACAGCAC TAATGGTTTG 
GAGGAGAGAA AGTTGCAGTT GCCTGCATCT ACTTGGAGTG ATGGTAGCTC AGTTCGTCCT 
AGCTACTGGC TGGTTCTTCA CAATCTCACT CCACAGATCG ATGGGTCAAC CTTGAGGACG 
ATCTGCATGC AGCATGGTCC ACTGCTGACA TTCCATCTGA ACCTGACCCA GGGCACCGCT 
CTCATCCGAT ACAACACCAA ACAGGAGGCG GCCAAGGCCC AGACTGCACT GCACATGTGT 
GTGTTGGGAA ACACTACCAT CCTTGCTGAG TTTGCCACAG ATGAAGAGGT TAGTCGCTTT 
TTGACACAAG CTCAGCCCCC TACACCTGCA GCAACCCCAA GTGCACCCAC GGCAGGCTGG 
CAATCCCTGG AGACAGGACA GAACCAGACA GATCCAGTTG GACCTGCTTT GAATCTCTTT 
GGTGGGTCAA CAGGGCTTGG GCAGTGGAGC AGTGGTGGCG GCGGCAGCAG TGGGGGTGAT 
CTCACTGGCG CTTCGTTGTG GGGGCCCCCA AACTATTCTT CAAGCTTGTG GGGGGTCCCA 
AGCGTAGAGG ATCCACACAG AATGGGCAGC CCTGCTCCCT TACTACCTGG AGACCTTCTG 
GGAGGAGGGT CGGATTCAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_004937724.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004937781.2 Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X3, mRNA.

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CloneID	OGa29592
Clone ID Related Accession (Same CDS sequence)	XM_004937724.2
Accession Version	XM_004937724.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5595bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	trinucleotide repeat-containing gene 6B protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455714.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004937724.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACTG ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGGTCAG TGAGATGGAA 
CATGAGGATT TTGTAATGGA GGGGCATGGC AAGACTCCAC CTCCTGGTGA AGAAAACAAA 
CAAGAGAAGG AGCAAGAAAG GGAAGAACAG TTAATGGAAG ACAAGAAAAG GAAGAAGGAG 
GATAAGAAAA AGAAGGAATC TGCTCAGAAG GTCACAGATC AAAAAACCAA AGTGCCCGAA 
GTGACGAAAC CAAGTTTAAG CCAACCAGTG GCTGCCAGCC CAATTGGCAA CTCTCCATCG 
CCACCAGTCA ATGGTGGCAA CAATGCCAAA AGGGTGGCAG TGCCGAACGG ACAACCGCCA 
AGCGCCGCCC GCTACATGCC TCGGGAGGTG CCGCCGCGAT TCCGTTGCCA GCAGGACCAC 
AAAGTGTTAC TGAAACGTGG GCAGCCCCCT CCACCATCCT GTATGCTCCT TGGGGGTGGA 
GCAGGGCCTC CTCCCCCTAC AGCACCAGGA GCAAACCCAA ACAACGCACA ACCAGTGACA 
GGAGCACTGC TGCAGAGCGA CAGTGGGACT GCAACAGATT CAACAATTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGT CCTGGAGCCT CCTCCAGCAG CGGCACCAAC 
GCCAACCCAA CTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGTATCG CCAGCAAAGA TGCTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCGAACC CTGGCTTGGA GAAGAACACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGAAGCC AGTGCCAGTC TGGAAGCGCT GGAAATGAAT GTAATCTTGG GGCCTGGAAA 
TCTGATCCCA AGGCTAAATC TGTTCAATCT TCCAACCCTG CTACCGAGAG TAACAGTGGA 
CTAAGTAATT GGAGGAACTT GACTGGACAA GATAGAATTG GTTCTGGTTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCGTCTGCT TGGCCAGCAC TGGTCCAAGA GGGCAATTCT 
AGGAAAGGGA CTTCGGAATC TGATAGTGGT AGCTCCAATG CACAGATTAG CACAGTAGGT 
CAGACCTCTA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAACTT TGTCTCTGGC 
AGAGAACAGG CTCAAATTCA TAACACTGAT GGACCAAAAA ATGGAAACAC TAACTCCTTG 
AACTTAAGTT CACCAAACCC TATGGAGAAT AAGGGAATGC CCTTTGAAAT GGGCTTGGGG 
AACCCTTCCA GGAGCACTGA CACCCCTTCA CAAAGCACTG GAGAAAGAAA GACTGGGAGT 
GTTGGATCTT GGGGTACAGC TAGGGGGTCT TCTGGAACTG ACACAGTCTC TGGACAGAGC 
AATTCTGGAA ACCATGGGAA CAATGGAAAG GATAGGGAGG ACTCCTGGAA AGGAGCTTCT 
GTTCAAAAAC CTAATGGGTC AAGAAGCGAT TCTTGGGATA ACAATAACCG GTCTACGGGT 
GGTGGGTCTT GGAACTTTGG CCCTCAGCAT GCAAATGAAA GCAAATGGGG TGAAGGGAAC 
AAATTAACAT CTGGGGTCTC TCAGGGAGAA TGGAAACAGC TGTCTGGGTC TGATGAACTG 
AAGATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAAAAAGGCC ACCCTCTTCC TGAAAACCAA GGCAATTCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CTGCAGGAAG TGAGGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGACGC CGATCATACA GGCCTACGCA TCCTGATTGC 
CAGGCAGTCT TGCAGACTTT GCTGAGCAGG ACTGATTTGG ACCCCCGAGT GCTTTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAA GATACCGTAT GGGATATTGA AGAGATGCCA 
CGGTCCGAGG GGAAGTCTGA TAAAGGAACT GAGGGGTGGG AGAGCTCTGC CACACAGACA 
AAGAACTCAG GGGGCTGGGG CGATGTACCC AGCCAAAGCA ATCAAATCAA GTCTGGATGG 
GGTGAGCTCT CAACCCCAAC AGAATGGAAG GACCCCAAAA ATACTGGAGG ATGGAATGAC 
TATAAGAACC CCAATTCGTC TAATTGGGGA GGGGGAAGAC AAGAAGAGAA ATCGTCATCT 
TCTTGGAATG ACAGCTCTAG TAAGGATCAG GGGTGGGGTG GACGGCAGCC TAATCAAGGA 
TGGTCTTCTG GAAAGAATGG TTGGGGAGAG GAAGTTGACC AAGCAAAAAA CAGCAACTGG 
GAAAGTGCGG GAAATAAGCC AGCATCTGGT TGGGGTGAAG GTGGGCAAAA TGAGATTGGA 
ACTTGGGGTA ACGGTGCAAA TACAGGCTCT GCTTCAAAGG GTGGTTGGGA TGATTGTAAA 
AGAAATTCAA CGTGGAACGA GACGGGTCGA CAGCCCAACT CCTGGAACAA GCAGCAGCAG 
CAGCAACAGC AGCAACAGCA AGAGACTTCT GGTTCCTGGG GTCCACCGCC ACAAACTCCA 
AGTAATGGGC GACCTCCAAA CCCAAACTGG AACAGCGGAC CACAACCAGC TGCCCCCAAA 
GACGAGGAGC CTAGTGGCTG GGAAGAACCT TCTCCACAGT CAATCAGTCG AAAAATGGAT 
ATTGATGATG GCACTTCAGC GTGGGGAGAT CCTAGCAGTT ATAACTACAA GAATGTGAAC 
CTGTGGGACA AGAACTCACA AGGGGGACAG GCCCCACGGG AACAGAGCCT GCCTACTCCA 
ATGACTAGCA AATCACAAGC ATCAGTCTGG AGCAAAAGCA CTCCACCTGC TCCAGATAAT 
GGTACGTCCG CCTGGGGTGA GCCAAATGAA ACCAGTCCCG GGTGGGGTGA AGTAGATGAT 
ACAGGAGCAT CGACAACAGG CTGGGGGAAT GCACCTTCCA ACGCCCCGAA TGCCATGAAA 
TCTAGTTCTA AATCTATGCA AGATGGCTGG GGAGAGAGTG ATGGGCCAGT GACAGGAACA 
CGTCATTCCA GCTGGGAAGA GGAGGAGGAG GGAGGAGTCT GGAACACAGC AGGCTCTCAG 
GGAAGCAGTT CCTCCCACAA CTCAACAAGC TGGGGGCAAG GAGGAAAGAA AACGCAGATG 
AAGTGCTCGT TAAAAGGAGG AAATAGTGAT TCATGGATGA ACCCTATATC CAAGCAGTTT 
TCAAATATGG GCTTGCTAAG TCAAACTGAG GACATTCCAG GCAATAAGAT GGACTTGTCT 
GTAGGTGGTC TCCCAGATAA GAAGTTTGAG GTAGATAAAC GAGCCATGAA TCTCGGGGAT 
TTTAATGACA TAATGAGAAA GGATCGATCT GGGTTCCGTC CACCTAATTC CAAAGACATG 
GGAACCACAG ATAGTGGGCC TTATTTTGAG AAGCTGACTT TGCCTTTCTC CAATCAAGAT 
GGGTGCCTTG GGGATGAGGC TCCCTGCTCT CCCTTCTCCC CTTCTCCCAG CTACAAGCTG 
TCTCCCTCTG GTTCCACACT ATCCAACGTC GGCCTTGGAG CAATCGGCTC AGGGCTCAGT 
CCCCAAAACT ACGCTGCTAG ACAAGGTGGC AATCATGGTT TGTTTGGAAA CAGCACAGCA 
CAATCGAGGG GCATGCACAC ACCAGTGCAG CCACTAAATC CTTCCCCCAA TATCCGGGCG 
CAAGTGCCTC CCCAATTTCT TTCTCCCCAG GTTTCGGCCT CCATGCTCAA ACAGTTCCCC 
AACAGTGGCT TGAATCCAGG TCTTTTCAAT GTGGGGCCCC AGCTTTCTCC ACAACAGATT 
GCCATGCTGA GCCAGCTTCC ACAGATTCCC CAGTTTCAAT TAGCATGTCA GCTCCTCCTT 
CAGCAGCAGC AGCAGCAGCA GCAGCTGTTA CAGAGCCAGA GGAAGTTATC TCAAGCTGTG 
CGACAGCAGC AGGAGCAACA GATTGCTCGT ATGGTGAGTG CCCTCCAGCA GCAGCAGCAG 
AGGCAGCCTG GCATGAAGCA TTCACCATCC CACCCTGTTG GGCCTAAGCC ACATATAGAC 
AGCATGGTAC CCAATCCTTT GAATGTGGGT CTTTCAGATT TACAGACCAA AGGACAAATA 
CCTGGATACG GTTCAGGCTT TGGCTCAAGC AGCATGGATT ATGGCATGGT GGGAGGGAAA 
GAAGCTGGAA CAGAATCCCG CTTTAAACAG TGGACTATGA TGGAAGGGCT GCCCTCTGTG 
GCTTCACAAG ATGCCAATAT GCACAAAAAT GGTGCAATAG TGCCCCCTGG AAAGGCTCGC 
GGAGGATCGC CCTACAACCA GTTCGACATA ATTCAGGGCG ACCCACTAGG TGGCCATGCA 
GGCCCTGCTG GTGATAGTTG GTTACCTGCC AAATCTCCAC CGACAAACAA GATCGGAAGC 
AAATCCAGCA ACGCCAGCTG GCCTCCAGAG TTCCAACCAG GAGTGCCATG GAAAGGTATA 
CAAAACATTG ACCCTGAATC TGACCCCTAC GTGACCCCTG GAAGTGTGCT GGGGGGGACA 
GCCACATCTC CCATTGTAGA TACTGACCAC CAACTTCTGC GGGACAACAC CACAGGGTCT 
AATTCTTCCC TCAACACCTC GCTGCCTTCA CCTGGTGCCT GGCCCTACAG TGCCTCTGAC 
AATTCCTTCA CCAACGTTCA TAGCACTTCA GCAAAATTCA GTGATTACAA ATCAACATGG 
TCCCCAGATC CCATAGGACA CAATCCCACT CATCTCTCCA ACAAGATGTG GAAAAACCAT 
ATTTCCTCAA GGAACACTAC AGGGCTGCCT CGCCCGCCTC CTGGCCTGAC CAACCCCAAA 
CCATCATCTC CATGGAACAG CACAGCACCC CGATCGGTCA GGGGCTGGGG GGCACAGGAC 
TCAAGGCTTG CCTCTGCATC TACTTGGAGT GATGGTAGCT CAGTTCGTCC TAGCTACTGG 
CTGGTTCTTC ACAATCTCAC TCCACAGATC GATGGGTCAA CCTTGAGGAC GATCTGCATG 
CAGCATGGTC CACTGCTGAC ATTCCATCTG AACCTGACCC AGGGCACCGC TCTCATCCGA 
TACAACACCA AACAGGAGGC GGCCAAGGCC CAGACTGCAC TGCACATGTG TGTGTTGGGA 
AACACTACCA TCCTTGCTGA GTTTGCCACA GATGAAGAGG TTAGTCGCTT TTTGACACAA 
GCTCAGCCCC CTACACCTGC AGCAACCCCA AGTGCACCCA CGGCAGGCTG GCAATCCCTG 
GAGACAGGAC AGAACCAGAC AGATCCAGTT GGACCTGCTT TGAATCTCTT TGGTGGGTCA 
ACAGGGCTTG GGCAGTGGAG CAGTGGTGGC GGCGGCAGCA GTGGGGGTGA TCTCACTGGC 
GCTTCGTTGT GGGGGCCCCC AAACTATTCT TCAAGCTTGT GGGGGGTCCC AAGCGTAGAG 
GATCCACACA GAATGGGCAG CCCTGCTCCC TTACTACCTG GAGACCTTCT GGGAGGAGGG 
TCGGATTCAA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004937781.2
CDS	538..6132
Translation	MQTDEGEILEEGSPKVSEMEHEDFVMEGHGKTPPPGEENKQEKEQEREEQLMEDKKRKKEDKKKKESAQKVTDQKTKVPEVTKPSLSQPVAASPIGNSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPPTAPGANPNNAQPVTGALLQSDSGTATDSTIGGAAASNYANSTWGPGASSSSGTNANPTHIWDKVIVDGSDMEEWPCIASKDAESSSENTTDNNSASNPGLEKNTLPGSTTSNKGKGSQCQSGSAGNECNLGAWKSDPKAKSVQSSNPATESNSGLSNWRNLTGQDRIGSGSGFSNFNPNSNPSAWPALVQEGNSRKGTSESDSGSSNAQISTVGQTSREQQSKMENAGVNFVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFEMGLGNPSRSTDTPSQSTGERKTGSVGSWGTARGSSGTDTVSGQSNSGNHGNNGKDREDSWKGASVQKPNGSRSDSWDNNNRSTGGGSWNFGPQHANESKWGEGNKLTSGVSQGEWKQLSGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNSQAPCWGRSSSSAGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEMPRSEGKSDKGTEGWESSATQTKNSGGWGDVPSQSNQIKSGWGELSTPTEWKDPKNTGGWNDYKNPNSSNWGGGRQEEKSSSSWNDSSSKDQGWGGRQPNQGWSSGKNGWGEEVDQAKNSNWESAGNKPASGWGEGGQNEIGTWGNGANTGSASKGGWDDCKRNSTWNETGRQPNSWNKQQQQQQQQQQETSGSWGPPPQTPSNGRPPNPNWNSGPQPAAPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPSSYNYKNVNLWDKNSQGGQAPREQSLPTPMTSKSQASVWSKSTPPAPDNGTSAWGEPNETSPGWGEVDDTGASTTGWGNAPSNAPNAMKSSSKSMQDGWGESDGPVTGTRHSSWEEEEEGGVWNTAGSQGSSSSHNSTSWGQGGKKTQMKCSLKGGNSDSWMNPISKQFSNMGLLSQTEDIPGNKMDLSVGGLPDKKFEVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLSNVGLGAIGSGLSPQNYAARQGGNHGLFGNSTAQSRGMHTPVQPLNPSPNIRAQVPPQFLSPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQQLLQSQRKLSQAVRQQQEQQIARMVSALQQQQQRQPGMKHSPSHPVGPKPHIDSMVPNPLNVGLSDLQTKGQIPGYGSGFGSSSMDYGMVGGKEAGTESRFKQWTMMEGLPSVASQDANMHKNGAIVPPGKARGGSPYNQFDIIQGDPLGGHAGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFSDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTGLPRPPPGLTNPKPSSPWNSTAPRSVRGWGAQDSRLASASTWSDGSSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYNTKQEAAKAQTALHMCVLGNTTILAEFATDEEVSRFLTQAQPPTPAATPSAPTAGWQSLETGQNQTDPVGPALNLFGGSTGLGQWSSGGGGSSGGDLTGASLWGPPNYSSSLWGVPSVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_004937724.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004937724.2

ORF Insert Sequence:

ATGCAAACTG ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGGTCAG TGAGATGGAA 
CATGAGGATT TTGTAATGGA GGGGCATGGC AAGACTCCAC CTCCTGGTGA AGAAAACAAA 
CAAGAGAAGG AGCAAGAAAG GGAAGAACAG TTAATGGAAG ACAAGAAAAG GAAGAAGGAG 
GATAAGAAAA AGAAGGAATC TGCTCAGAAG GTCACAGATC AAAAAACCAA AGTGCCCGAA 
GTGACGAAAC CAAGTTTAAG CCAACCAGTG GCTGCCAGCC CAATTGGCAA CTCTCCATCG 
CCACCAGTCA ATGGTGGCAA CAATGCCAAA AGGGTGGCAG TGCCGAACGG ACAACCGCCA 
AGCGCCGCCC GCTACATGCC TCGGGAGGTG CCGCCGCGAT TCCGTTGCCA GCAGGACCAC 
AAAGTGTTAC TGAAACGTGG GCAGCCCCCT CCACCATCCT GTATGCTCCT TGGGGGTGGA 
GCAGGGCCTC CTCCCCCTAC AGCACCAGGA GCAAACCCAA ACAACGCACA ACCAGTGACA 
GGAGCACTGC TGCAGAGCGA CAGTGGGACT GCAACAGATT CAACAATTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGT CCTGGAGCCT CCTCCAGCAG CGGCACCAAC 
GCCAACCCAA CTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGTATCG CCAGCAAAGA TGCTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCGAACC CTGGCTTGGA GAAGAACACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGAAGCC AGTGCCAGTC TGGAAGCGCT GGAAATGAAT GTAATCTTGG GGCCTGGAAA 
TCTGATCCCA AGGCTAAATC TGTTCAATCT TCCAACCCTG CTACCGAGAG TAACAGTGGA 
CTAAGTAATT GGAGGAACTT GACTGGACAA GATAGAATTG GTTCTGGTTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCGTCTGCT TGGCCAGCAC TGGTCCAAGA GGGCAATTCT 
AGGAAAGGGA CTTCGGAATC TGATAGTGGT AGCTCCAATG CACAGATTAG CACAGTAGGT 
CAGACCTCTA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAACTT TGTCTCTGGC 
AGAGAACAGG CTCAAATTCA TAACACTGAT GGACCAAAAA ATGGAAACAC TAACTCCTTG 
AACTTAAGTT CACCAAACCC TATGGAGAAT AAGGGAATGC CCTTTGAAAT GGGCTTGGGG 
AACCCTTCCA GGAGCACTGA CACCCCTTCA CAAAGCACTG GAGAAAGAAA GACTGGGAGT 
GTTGGATCTT GGGGTACAGC TAGGGGGTCT TCTGGAACTG ACACAGTCTC TGGACAGAGC 
AATTCTGGAA ACCATGGGAA CAATGGAAAG GATAGGGAGG ACTCCTGGAA AGGAGCTTCT 
GTTCAAAAAC CTAATGGGTC AAGAAGCGAT TCTTGGGATA ACAATAACCG GTCTACGGGT 
GGTGGGTCTT GGAACTTTGG CCCTCAGCAT GCAAATGAAA GCAAATGGGG TGAAGGGAAC 
AAATTAACAT CTGGGGTCTC TCAGGGAGAA TGGAAACAGC TGTCTGGGTC TGATGAACTG 
AAGATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAAAAAGGCC ACCCTCTTCC TGAAAACCAA GGCAATTCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CTGCAGGAAG TGAGGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGACGC CGATCATACA GGCCTACGCA TCCTGATTGC 
CAGGCAGTCT TGCAGACTTT GCTGAGCAGG ACTGATTTGG ACCCCCGAGT GCTTTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAA GATACCGTAT GGGATATTGA AGAGATGCCA 
CGGTCCGAGG GGAAGTCTGA TAAAGGAACT GAGGGGTGGG AGAGCTCTGC CACACAGACA 
AAGAACTCAG GGGGCTGGGG CGATGTACCC AGCCAAAGCA ATCAAATCAA GTCTGGATGG 
GGTGAGCTCT CAACCCCAAC AGAATGGAAG GACCCCAAAA ATACTGGAGG ATGGAATGAC 
TATAAGAACC CCAATTCGTC TAATTGGGGA GGGGGAAGAC AAGAAGAGAA ATCGTCATCT 
TCTTGGAATG ACAGCTCTAG TAAGGATCAG GGGTGGGGTG GACGGCAGCC TAATCAAGGA 
TGGTCTTCTG GAAAGAATGG TTGGGGAGAG GAAGTTGACC AAGCAAAAAA CAGCAACTGG 
GAAAGTGCGG GAAATAAGCC AGCATCTGGT TGGGGTGAAG GTGGGCAAAA TGAGATTGGA 
ACTTGGGGTA ACGGTGCAAA TACAGGCTCT GCTTCAAAGG GTGGTTGGGA TGATTGTAAA 
AGAAATTCAA CGTGGAACGA GACGGGTCGA CAGCCCAACT CCTGGAACAA GCAGCAGCAG 
CAGCAACAGC AGCAACAGCA AGAGACTTCT GGTTCCTGGG GTCCACCGCC ACAAACTCCA 
AGTAATGGGC GACCTCCAAA CCCAAACTGG AACAGCGGAC CACAACCAGC TGCCCCCAAA 
GACGAGGAGC CTAGTGGCTG GGAAGAACCT TCTCCACAGT CAATCAGTCG AAAAATGGAT 
ATTGATGATG GCACTTCAGC GTGGGGAGAT CCTAGCAGTT ATAACTACAA GAATGTGAAC 
CTGTGGGACA AGAACTCACA AGGGGGACAG GCCCCACGGG AACAGAGCCT GCCTACTCCA 
ATGACTAGCA AATCACAAGC ATCAGTCTGG AGCAAAAGCA CTCCACCTGC TCCAGATAAT 
GGTACGTCCG CCTGGGGTGA GCCAAATGAA ACCAGTCCCG GGTGGGGTGA AGTAGATGAT 
ACAGGAGCAT CGACAACAGG CTGGGGGAAT GCACCTTCCA ACGCCCCGAA TGCCATGAAA 
TCTAGTTCTA AATCTATGCA AGATGGCTGG GGAGAGAGTG ATGGGCCAGT GACAGGAACA 
CGTCATTCCA GCTGGGAAGA GGAGGAGGAG GGAGGAGTCT GGAACACAGC AGGCTCTCAG 
GGAAGCAGTT CCTCCCACAA CTCAACAAGC TGGGGGCAAG GAGGAAAGAA AACGCAGATG 
AAGTGCTCGT TAAAAGGAGG AAATAGTGAT TCATGGATGA ACCCTATATC CAAGCAGTTT 
TCAAATATGG GCTTGCTAAG TCAAACTGAG GACATTCCAG GCAATAAGAT GGACTTGTCT 
GTAGGTGGTC TCCCAGATAA GAAGTTTGAG GTAGATAAAC GAGCCATGAA TCTCGGGGAT 
TTTAATGACA TAATGAGAAA GGATCGATCT GGGTTCCGTC CACCTAATTC CAAAGACATG 
GGAACCACAG ATAGTGGGCC TTATTTTGAG AAGCTGACTT TGCCTTTCTC CAATCAAGAT 
GGGTGCCTTG GGGATGAGGC TCCCTGCTCT CCCTTCTCCC CTTCTCCCAG CTACAAGCTG 
TCTCCCTCTG GTTCCACACT ATCCAACGTC GGCCTTGGAG CAATCGGCTC AGGGCTCAGT 
CCCCAAAACT ACGCTGCTAG ACAAGGTGGC AATCATGGTT TGTTTGGAAA CAGCACAGCA 
CAATCGAGGG GCATGCACAC ACCAGTGCAG CCACTAAATC CTTCCCCCAA TATCCGGGCG 
CAAGTGCCTC CCCAATTTCT TTCTCCCCAG GTTTCGGCCT CCATGCTCAA ACAGTTCCCC 
AACAGTGGCT TGAATCCAGG TCTTTTCAAT GTGGGGCCCC AGCTTTCTCC ACAACAGATT 
GCCATGCTGA GCCAGCTTCC ACAGATTCCC CAGTTTCAAT TAGCATGTCA GCTCCTCCTT 
CAGCAGCAGC AGCAGCAGCA GCAGCTGTTA CAGAGCCAGA GGAAGTTATC TCAAGCTGTG 
CGACAGCAGC AGGAGCAACA GATTGCTCGT ATGGTGAGTG CCCTCCAGCA GCAGCAGCAG 
AGGCAGCCTG GCATGAAGCA TTCACCATCC CACCCTGTTG GGCCTAAGCC ACATATAGAC 
AGCATGGTAC CCAATCCTTT GAATGTGGGT CTTTCAGATT TACAGACCAA AGGACAAATA 
CCTGGATACG GTTCAGGCTT TGGCTCAAGC AGCATGGATT ATGGCATGGT GGGAGGGAAA 
GAAGCTGGAA CAGAATCCCG CTTTAAACAG TGGACTATGA TGGAAGGGCT GCCCTCTGTG 
GCTTCACAAG ATGCCAATAT GCACAAAAAT GGTGCAATAG TGCCCCCTGG AAAGGCTCGC 
GGAGGATCGC CCTACAACCA GTTCGACATA ATTCAGGGCG ACCCACTAGG TGGCCATGCA 
GGCCCTGCTG GTGATAGTTG GTTACCTGCC AAATCTCCAC CGACAAACAA GATCGGAAGC 
AAATCCAGCA ACGCCAGCTG GCCTCCAGAG TTCCAACCAG GAGTGCCATG GAAAGGTATA 
CAAAACATTG ACCCTGAATC TGACCCCTAC GTGACCCCTG GAAGTGTGCT GGGGGGGACA 
GCCACATCTC CCATTGTAGA TACTGACCAC CAACTTCTGC GGGACAACAC CACAGGGTCT 
AATTCTTCCC TCAACACCTC GCTGCCTTCA CCTGGTGCCT GGCCCTACAG TGCCTCTGAC 
AATTCCTTCA CCAACGTTCA TAGCACTTCA GCAAAATTCA GTGATTACAA ATCAACATGG 
TCCCCAGATC CCATAGGACA CAATCCCACT CATCTCTCCA ACAAGATGTG GAAAAACCAT 
ATTTCCTCAA GGAACACTAC AGGGCTGCCT CGCCCGCCTC CTGGCCTGAC CAACCCCAAA 
CCATCATCTC CATGGAACAG CACAGCACCC CGATCGGTCA GGGGCTGGGG GGCACAGGAC 
TCAAGGCTTG CCTCTGCATC TACTTGGAGT GATGGTAGCT CAGTTCGTCC TAGCTACTGG 
CTGGTTCTTC ACAATCTCAC TCCACAGATC GATGGGTCAA CCTTGAGGAC GATCTGCATG 
CAGCATGGTC CACTGCTGAC ATTCCATCTG AACCTGACCC AGGGCACCGC TCTCATCCGA 
TACAACACCA AACAGGAGGC GGCCAAGGCC CAGACTGCAC TGCACATGTG TGTGTTGGGA 
AACACTACCA TCCTTGCTGA GTTTGCCACA GATGAAGAGG TTAGTCGCTT TTTGACACAA 
GCTCAGCCCC CTACACCTGC AGCAACCCCA AGTGCACCCA CGGCAGGCTG GCAATCCCTG 
GAGACAGGAC AGAACCAGAC AGATCCAGTT GGACCTGCTT TGAATCTCTT TGGTGGGTCA 
ACAGGGCTTG GGCAGTGGAG CAGTGGTGGC GGCGGCAGCA GTGGGGGTGA TCTCACTGGC 
GCTTCGTTGT GGGGGCCCCC AAACTATTCT TCAAGCTTGT GGGGGGTCCC AAGCGTAGAG 
GATCCACACA GAATGGGCAG CCCTGCTCCC TTACTACCTG GAGACCTTCT GGGAGGAGGG 
TCGGATTCAA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_015288794.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015144280.1 Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X4, mRNA.

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CloneID	OGa29593
Clone ID Related Accession (Same CDS sequence)	XM_015288794.2 , XM_015288794.1
Accession Version	XM_015288794.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	trinucleotide repeat-containing gene 6B protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288794.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA AGAAAAGGAA GAAGGAGGAT AAGAAAAAGA AGGAATCTGC TCAGAAGGTC 
ACAGATCAAA AAACCAAAGT GCCCGAAGTG ACGAAACCAA GTTTAAGCCA ACCAGTGGCT 
GCCAGCCCAA TTGGCAACTC TCCATCGCCA CCAGTCAATG GTGGCAACAA TGCCAAAAGG 
GTGGCAGTGC CGAACGGACA ACCGCCAAGC GCCGCCCGCT ACATGCCTCG GGAGGTGCCG 
CCGCGATTCC GTTGCCAGCA GGACCACAAA GTGTTACTGA AACGTGGGCA GCCCCCTCCA 
CCATCCTGTA TGCTCCTTGG GGGTGGAGCA GGGCCTCCTC CCCCTACAGC ACCAGGAGCA 
AACCCAAACA ACGCACAACC AGTGACAGGA GCACTGCTGC AGAGCGACAG TGGGACTGCA 
ACAGATTCAA CAATTGGAGG TGCTGCTGCT TCAAATTATG CAAATTCCAC TTGGGGTCCT 
GGAGCCTCCT CCAGCAGCGG CACCAACGCC AACCCAACTC ACATCTGGGA CAAGGTGATT 
GTAGACGGGT CTGACATGGA AGAGTGGCCT TGTATCGCCA GCAAAGATGC TGAGTCTTCT 
TCCGAAAACA CCACCGATAA CAACAGTGCC TCGAACCCTG GCTTGGAGAA GAACACTCTG 
CCAGGAAGCA CCACTAGTAA CAAAGGAAAA GGAAGCCAGT GCCAGTCTGG AAGCGCTGGA 
AATGAATGTA ATCTTGGGGC CTGGAAATCT GATCCCAAGG CTAAATCTGT TCAATCTTCC 
AACCCTGCTA CCGAGAGTAA CAGTGGACTA AGTAATTGGA GGAACTTGAC TGGACAAGAT 
AGAATTGGTT CTGGTTCTGG CTTCAGCAAC TTTAACCCAA ATAGCAACCC GTCTGCTTGG 
CCAGCACTGG TCCAAGAGGG CAATTCTAGG AAAGGGACTT CGGAATCTGA TAGTGGTAGC 
TCCAATGCAC AGATTAGCAC AGTAGGTCAG ACCTCTAGGG AACAGCAGTC AAAGATGGAA 
AATGCGGGTG TTAACTTTGT CTCTGGCAGA GAACAGGCTC AAATTCATAA CACTGATGGA 
CCAAAAAATG GAAACACTAA CTCCTTGAAC TTAAGTTCAC CAAACCCTAT GGAGAATAAG 
GGAATGCCCT TTGAAATGGG CTTGGGGAAC CCTTCCAGGA GCACTGACAC CCCTTCACAA 
AGCACTGGAG AAAGAAAGAC TGGGAGTGTT GGATCTTGGG GTACAGCTAG GGGGTCTTCT 
GGAACTGACA CAGTCTCTGG ACAGAGCAAT TCTGGAAACC ATGGGAACAA TGGAAAGGAT 
AGGGAGGACT CCTGGAAAGG AGCTTCTGTT CAAAAACCTA ATGGGTCAAG AAGCGATTCT 
TGGGATAACA ATAACCGGTC TACGGGTGGT GGGTCTTGGA ACTTTGGCCC TCAGCATGCA 
AATGAAAGCA AATGGGGTGA AGGGAACAAA TTAACATCTG GGGTCTCTCA GGGAGAATGG 
AAACAGCTGT CTGGGTCTGA TGAACTGAAG ATTGGAGAAT GGAGTGGTCC AAACCAACCA 
AATTCTAGCA CTGGAGCATG GGACAATCAA AAAGGCCACC CTCTTCCTGA AAACCAAGGC 
AATTCCCAGG CTCCCTGTTG GGGAAGATCT TCCAGCTCTG CAGGAAGTGA GGTTGGAGGT 
CAAAGCACTG GAAGCAACCA CAAAGCAGGA AGTAGTGACA GTCACAATTC TGGACGCCGA 
TCATACAGGC CTACGCATCC TGATTGCCAG GCAGTCTTGC AGACTTTGCT GAGCAGGACT 
GATTTGGACC CCCGAGTGCT TTCAAACACT GGCTGGGGCC AAACTCAAAT TAAGCAAGAT 
ACCGTATGGG ATATTGAAGA GATGCCACGG TCCGAGGGGA AGTCTGATAA AGGAACTGAG 
GGGTGGGAGA GCTCTGCCAC ACAGACAAAG AACTCAGGGG GCTGGGGCGA TGTACCCAGC 
CAAAGCAATC AAATCAAGTC TGGATGGGGT GAGCTCTCAA CCCCAACAGA ATGGAAGGAC 
CCCAAAAATA CTGGAGGATG GAATGACTAT AAGAACCCCA ATTCGTCTAA TTGGGGAGGG 
GGAAGACAAG AAGAGAAATC GTCATCTTCT TGGAATGACA GCTCTAGTAA GGATCAGGGG 
TGGGGTGGAC GGCAGCCTAA TCAAGGATGG TCTTCTGGAA AGAATGGTTG GGGAGAGGAA 
GTTGACCAAG CAAAAAACAG CAACTGGGAA AGTGCGGGAA ATAAGCCAGC ATCTGGTTGG 
GGTGAAGGTG GGCAAAATGA GATTGGAACT TGGGGTAACG GTGCAAATAC AGGCTCTGCT 
TCAAAGGGTG GTTGGGATGA TTGTAAAAGA AATTCAACGT GGAACGAGAC GGGTCGACAG 
CCCAACTCCT GGAACAAGCA GCAGCAGCAG CAACAGCAGC AACAGCAAGA GACTTCTGGT 
TCCTGGGGTC CACCGCCACA AACTCCAAGT AATGGGCGAC CTCCAAACCC AAACTGGAAC 
AGCGGACCAC AACCAGCTGC CCCCAAAGAC GAGGAGCCTA GTGGCTGGGA AGAACCTTCT 
CCACAGTCAA TCAGTCGAAA AATGGATATT GATGATGGCA CTTCAGCGTG GGGAGATCCT 
AGCAGTTATA ACTACAAGAA TGTGAACCTG TGGGACAAGA ACTCACAAGG GGGACAGGCC 
CCACGGGAAC AGAGCCTGCC TACTCCAATG ACTAGCAAAT CACAAGCATC AGTCTGGAGC 
AAAAGCACTC CACCTGCTCC AGATAATGGT ACGTCCGCCT GGGGTGAGCC AAATGAAACC 
AGTCCCGGGT GGGGTGAAGT AGATGATACA GGAGCATCGA CAACAGGCTG GGGGAATGCA 
CCTTCCAACG CCCCGAATGC CATGAAATCT AGTTCTAAAT CTATGCAAGA TGGCTGGGGA 
GAGAGTGATG GGCCAGTGAC AGGAACACGT CATTCCAGCT GGGAAGAGGA GGAGGAGGGA 
GGAGTCTGGA ACACAGCAGG CTCTCAGGGA AGCAGTTCCT CCCACAACTC AACAAGCTGG 
GGGCAAGGAG GAAAGAAAAC GCAGATGAAG TGCTCGTTAA AAGGAGGAAA TAGTGATTCA 
TGGATGAACC CTATATCCAA GCAGTTTTCA AATATGGGCT TGCTAAGTCA AACTGAGGAC 
ATTCCAGGCA ATAAGATGGA CTTGTCTGTA GGTGGTCTCC CAGATAAGAA GTTTGAGGTA 
GATAAACGAG CCATGAATCT CGGGGATTTT AATGACATAA TGAGAAAGGA TCGATCTGGG 
TTCCGTCCAC CTAATTCCAA AGACATGGGA ACCACAGATA GTGGGCCTTA TTTTGAGAAG 
CTGACTTTGC CTTTCTCCAA TCAAGATGGG TGCCTTGGGG ATGAGGCTCC CTGCTCTCCC 
TTCTCCCCTT CTCCCAGCTA CAAGCTGTCT CCCTCTGGTT CCACACTATC CAACGTCGGC 
CTTGGAGCAA TCGGCTCAGG GCTCAGTCCC CAAAACTACG CTGCTAGACA AGGTGGCAAT 
CATGGTTTGT TTGGAAACAG CACAGCACAA TCGAGGGGCA TGCACACACC AGTGCAGCCA 
CTAAATCCTT CCCCCAATAT CCGGGCGCAA GTGCCTCCCC AATTTCTTTC TCCCCAGGTT 
TCGGCCTCCA TGCTCAAACA GTTCCCCAAC AGTGGCTTGA ATCCAGGTCT TTTCAATGTG 
GGGCCCCAGC TTTCTCCACA ACAGATTGCC ATGCTGAGCC AGCTTCCACA GATTCCCCAG 
TTTCAATTAG CATGTCAGCT CCTCCTTCAG CAGCAGCAGC AGCAGCAGCA GCTGTTACAG 
AGCCAGAGGA AGTTATCTCA AGCTGTGCGA CAGCAGCAGG AGCAACAGAT TGCTCGTATG 
GTGAGTGCCC TCCAGCAGCA GCAGCAGAGG CAGCCTGGCA TGAAGCATTC ACCATCCCAC 
CCTGTTGGGC CTAAGCCACA TATAGACAGC ATGGTACCCA ATCCTTTGAA TGTGGGTCTT 
TCAGATTTAC AGACCAAAGG ACAAATACCT GGATACGGTT CAGGCTTTGG CTCAAGCAGC 
ATGGATTATG GCATGGTGGG AGGGAAAGAA GCTGGAACAG AATCCCGCTT TAAACAGTGG 
ACTATGATGG AAGGGCTGCC CTCTGTGGCT TCACAAGATG CCAATATGCA CAAAAATGGT 
GCAATAGTGC CCCCTGGAAA GGCTCGCGGA GGATCGCCCT ACAACCAGTT CGACATAATT 
CAGGGCGACC CACTAGGTGG CCATGCAGGC CCTGCTGGTG ATAGTTGGTT ACCTGCCAAA 
TCTCCACCGA CAAACAAGAT CGGAAGCAAA TCCAGCAACG CCAGCTGGCC TCCAGAGTTC 
CAACCAGGAG TGCCATGGAA AGGTATACAA AACATTGACC CTGAATCTGA CCCCTACGTG 
ACCCCTGGAA GTGTGCTGGG GGGGACAGCC ACATCTCCCA TTGTAGATAC TGACCACCAA 
CTTCTGCGGG ACAACACCAC AGGGTCTAAT TCTTCCCTCA ACACCTCGCT GCCTTCACCT 
GGTGCCTGGC CCTACAGTGC CTCTGACAAT TCCTTCACCA ACGTTCATAG CACTTCAGCA 
AAATTCAGTG ATTACAAATC AACATGGTCC CCAGATCCCA TAGGACACAA TCCCACTCAT 
CTCTCCAACA AGATGTGGAA AAACCATATT TCCTCAAGGA ACACTACAGG GCTGCCTCGC 
CCGCCTCCTG GCCTGACCAA CCCCAAACCA TCATCTCCAT GGAACAGCAC AGCACCCCGA 
TCGGTCAGGG GCTGGGGGGC ACAGGACTCA AGGCTTGCCT CTGGTGAGAA GGATCATCTG 
ATAACGCCAT TGATCAGACC TGAGGAGTGT GCATTTTACA GCACTAATGG TTTGGAGGAG 
AGAAAGTTGC AGTTGCCTGC ATCTACTTGG AGTGATGGTA GCTCAGTTCG TCCTAGCTAC 
TGGCTGGTTC TTCACAATCT CACTCCACAG ATCGATGGGT CAACCTTGAG GACGATCTGC 
ATGCAGCATG GTCCACTGCT GACATTCCAT CTGAACCTGA CCCAGGGCAC CGCTCTCATC 
CGATACAACA CCAAACAGGA GGCGGCCAAG GCCCAGACTG CACTGCACAT GTGTGTGTTG 
GGAAACACTA CCATCCTTGC TGAGTTTGCC ACAGATGAAG AGGTTAGTCG CTTTTTGACA 
CAAGCTCAGC CCCCTACACC TGCAGCAACC CCAAGTGCAC CCACGGCAGG CTGGCAATCC 
CTGGAGACAG GACAGAACCA GACAGATCCA GTTGGACCTG CTTTGAATCT CTTTGGTGGG 
TCAACAGGGC TTGGGCAGTG GAGCAGTGGT GGCGGCGGCA GCAGTGGGGG TGATCTCACT 
GGCGCTTCGT TGTGGGGGCC CCCAAACTAT TCTTCAAGCT TGTGGGGGGT CCCAAGCGTA 
GAGGATCCAC ACAGAATGGG CAGCCCTGCT CCCTTACTAC CTGGAGACCT TCTGGGAGGA 
GGGTCGGATT CAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015144280.1
CDS	357..5894
Translation	MEDKKRKKEDKKKKESAQKVTDQKTKVPEVTKPSLSQPVAASPIGNSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPPTAPGANPNNAQPVTGALLQSDSGTATDSTIGGAAASNYANSTWGPGASSSSGTNANPTHIWDKVIVDGSDMEEWPCIASKDAESSSENTTDNNSASNPGLEKNTLPGSTTSNKGKGSQCQSGSAGNECNLGAWKSDPKAKSVQSSNPATESNSGLSNWRNLTGQDRIGSGSGFSNFNPNSNPSAWPALVQEGNSRKGTSESDSGSSNAQISTVGQTSREQQSKMENAGVNFVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFEMGLGNPSRSTDTPSQSTGERKTGSVGSWGTARGSSGTDTVSGQSNSGNHGNNGKDREDSWKGASVQKPNGSRSDSWDNNNRSTGGGSWNFGPQHANESKWGEGNKLTSGVSQGEWKQLSGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNSQAPCWGRSSSSAGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEMPRSEGKSDKGTEGWESSATQTKNSGGWGDVPSQSNQIKSGWGELSTPTEWKDPKNTGGWNDYKNPNSSNWGGGRQEEKSSSSWNDSSSKDQGWGGRQPNQGWSSGKNGWGEEVDQAKNSNWESAGNKPASGWGEGGQNEIGTWGNGANTGSASKGGWDDCKRNSTWNETGRQPNSWNKQQQQQQQQQQETSGSWGPPPQTPSNGRPPNPNWNSGPQPAAPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPSSYNYKNVNLWDKNSQGGQAPREQSLPTPMTSKSQASVWSKSTPPAPDNGTSAWGEPNETSPGWGEVDDTGASTTGWGNAPSNAPNAMKSSSKSMQDGWGESDGPVTGTRHSSWEEEEEGGVWNTAGSQGSSSSHNSTSWGQGGKKTQMKCSLKGGNSDSWMNPISKQFSNMGLLSQTEDIPGNKMDLSVGGLPDKKFEVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLSNVGLGAIGSGLSPQNYAARQGGNHGLFGNSTAQSRGMHTPVQPLNPSPNIRAQVPPQFLSPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQQLLQSQRKLSQAVRQQQEQQIARMVSALQQQQQRQPGMKHSPSHPVGPKPHIDSMVPNPLNVGLSDLQTKGQIPGYGSGFGSSSMDYGMVGGKEAGTESRFKQWTMMEGLPSVASQDANMHKNGAIVPPGKARGGSPYNQFDIIQGDPLGGHAGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFSDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTGLPRPPPGLTNPKPSSPWNSTAPRSVRGWGAQDSRLASGEKDHLITPLIRPEECAFYSTNGLEERKLQLPASTWSDGSSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYNTKQEAAKAQTALHMCVLGNTTILAEFATDEEVSRFLTQAQPPTPAATPSAPTAGWQSLETGQNQTDPVGPALNLFGGSTGLGQWSSGGGGSSGGDLTGASLWGPPNYSSSLWGVPSVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_015288794.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015288794.2

ORF Insert Sequence:

ATGGAAGACA AGAAAAGGAA GAAGGAGGAT AAGAAAAAGA AGGAATCTGC TCAGAAGGTC 
ACAGATCAAA AAACCAAAGT GCCCGAAGTG ACGAAACCAA GTTTAAGCCA ACCAGTGGCT 
GCCAGCCCAA TTGGCAACTC TCCATCGCCA CCAGTCAATG GTGGCAACAA TGCCAAAAGG 
GTGGCAGTGC CGAACGGACA ACCGCCAAGC GCCGCCCGCT ACATGCCTCG GGAGGTGCCG 
CCGCGATTCC GTTGCCAGCA GGACCACAAA GTGTTACTGA AACGTGGGCA GCCCCCTCCA 
CCATCCTGTA TGCTCCTTGG GGGTGGAGCA GGGCCTCCTC CCCCTACAGC ACCAGGAGCA 
AACCCAAACA ACGCACAACC AGTGACAGGA GCACTGCTGC AGAGCGACAG TGGGACTGCA 
ACAGATTCAA CAATTGGAGG TGCTGCTGCT TCAAATTATG CAAATTCCAC TTGGGGTCCT 
GGAGCCTCCT CCAGCAGCGG CACCAACGCC AACCCAACTC ACATCTGGGA CAAGGTGATT 
GTAGACGGGT CTGACATGGA AGAGTGGCCT TGTATCGCCA GCAAAGATGC TGAGTCTTCT 
TCCGAAAACA CCACCGATAA CAACAGTGCC TCGAACCCTG GCTTGGAGAA GAACACTCTG 
CCAGGAAGCA CCACTAGTAA CAAAGGAAAA GGAAGCCAGT GCCAGTCTGG AAGCGCTGGA 
AATGAATGTA ATCTTGGGGC CTGGAAATCT GATCCCAAGG CTAAATCTGT TCAATCTTCC 
AACCCTGCTA CCGAGAGTAA CAGTGGACTA AGTAATTGGA GGAACTTGAC TGGACAAGAT 
AGAATTGGTT CTGGTTCTGG CTTCAGCAAC TTTAACCCAA ATAGCAACCC GTCTGCTTGG 
CCAGCACTGG TCCAAGAGGG CAATTCTAGG AAAGGGACTT CGGAATCTGA TAGTGGTAGC 
TCCAATGCAC AGATTAGCAC AGTAGGTCAG ACCTCTAGGG AACAGCAGTC AAAGATGGAA 
AATGCGGGTG TTAACTTTGT CTCTGGCAGA GAACAGGCTC AAATTCATAA CACTGATGGA 
CCAAAAAATG GAAACACTAA CTCCTTGAAC TTAAGTTCAC CAAACCCTAT GGAGAATAAG 
GGAATGCCCT TTGAAATGGG CTTGGGGAAC CCTTCCAGGA GCACTGACAC CCCTTCACAA 
AGCACTGGAG AAAGAAAGAC TGGGAGTGTT GGATCTTGGG GTACAGCTAG GGGGTCTTCT 
GGAACTGACA CAGTCTCTGG ACAGAGCAAT TCTGGAAACC ATGGGAACAA TGGAAAGGAT 
AGGGAGGACT CCTGGAAAGG AGCTTCTGTT CAAAAACCTA ATGGGTCAAG AAGCGATTCT 
TGGGATAACA ATAACCGGTC TACGGGTGGT GGGTCTTGGA ACTTTGGCCC TCAGCATGCA 
AATGAAAGCA AATGGGGTGA AGGGAACAAA TTAACATCTG GGGTCTCTCA GGGAGAATGG 
AAACAGCTGT CTGGGTCTGA TGAACTGAAG ATTGGAGAAT GGAGTGGTCC AAACCAACCA 
AATTCTAGCA CTGGAGCATG GGACAATCAA AAAGGCCACC CTCTTCCTGA AAACCAAGGC 
AATTCCCAGG CTCCCTGTTG GGGAAGATCT TCCAGCTCTG CAGGAAGTGA GGTTGGAGGT 
CAAAGCACTG GAAGCAACCA CAAAGCAGGA AGTAGTGACA GTCACAATTC TGGACGCCGA 
TCATACAGGC CTACGCATCC TGATTGCCAG GCAGTCTTGC AGACTTTGCT GAGCAGGACT 
GATTTGGACC CCCGAGTGCT TTCAAACACT GGCTGGGGCC AAACTCAAAT TAAGCAAGAT 
ACCGTATGGG ATATTGAAGA GATGCCACGG TCCGAGGGGA AGTCTGATAA AGGAACTGAG 
GGGTGGGAGA GCTCTGCCAC ACAGACAAAG AACTCAGGGG GCTGGGGCGA TGTACCCAGC 
CAAAGCAATC AAATCAAGTC TGGATGGGGT GAGCTCTCAA CCCCAACAGA ATGGAAGGAC 
CCCAAAAATA CTGGAGGATG GAATGACTAT AAGAACCCCA ATTCGTCTAA TTGGGGAGGG 
GGAAGACAAG AAGAGAAATC GTCATCTTCT TGGAATGACA GCTCTAGTAA GGATCAGGGG 
TGGGGTGGAC GGCAGCCTAA TCAAGGATGG TCTTCTGGAA AGAATGGTTG GGGAGAGGAA 
GTTGACCAAG CAAAAAACAG CAACTGGGAA AGTGCGGGAA ATAAGCCAGC ATCTGGTTGG 
GGTGAAGGTG GGCAAAATGA GATTGGAACT TGGGGTAACG GTGCAAATAC AGGCTCTGCT 
TCAAAGGGTG GTTGGGATGA TTGTAAAAGA AATTCAACGT GGAACGAGAC GGGTCGACAG 
CCCAACTCCT GGAACAAGCA GCAGCAGCAG CAACAGCAGC AACAGCAAGA GACTTCTGGT 
TCCTGGGGTC CACCGCCACA AACTCCAAGT AATGGGCGAC CTCCAAACCC AAACTGGAAC 
AGCGGACCAC AACCAGCTGC CCCCAAAGAC GAGGAGCCTA GTGGCTGGGA AGAACCTTCT 
CCACAGTCAA TCAGTCGAAA AATGGATATT GATGATGGCA CTTCAGCGTG GGGAGATCCT 
AGCAGTTATA ACTACAAGAA TGTGAACCTG TGGGACAAGA ACTCACAAGG GGGACAGGCC 
CCACGGGAAC AGAGCCTGCC TACTCCAATG ACTAGCAAAT CACAAGCATC AGTCTGGAGC 
AAAAGCACTC CACCTGCTCC AGATAATGGT ACGTCCGCCT GGGGTGAGCC AAATGAAACC 
AGTCCCGGGT GGGGTGAAGT AGATGATACA GGAGCATCGA CAACAGGCTG GGGGAATGCA 
CCTTCCAACG CCCCGAATGC CATGAAATCT AGTTCTAAAT CTATGCAAGA TGGCTGGGGA 
GAGAGTGATG GGCCAGTGAC AGGAACACGT CATTCCAGCT GGGAAGAGGA GGAGGAGGGA 
GGAGTCTGGA ACACAGCAGG CTCTCAGGGA AGCAGTTCCT CCCACAACTC AACAAGCTGG 
GGGCAAGGAG GAAAGAAAAC GCAGATGAAG TGCTCGTTAA AAGGAGGAAA TAGTGATTCA 
TGGATGAACC CTATATCCAA GCAGTTTTCA AATATGGGCT TGCTAAGTCA AACTGAGGAC 
ATTCCAGGCA ATAAGATGGA CTTGTCTGTA GGTGGTCTCC CAGATAAGAA GTTTGAGGTA 
GATAAACGAG CCATGAATCT CGGGGATTTT AATGACATAA TGAGAAAGGA TCGATCTGGG 
TTCCGTCCAC CTAATTCCAA AGACATGGGA ACCACAGATA GTGGGCCTTA TTTTGAGAAG 
CTGACTTTGC CTTTCTCCAA TCAAGATGGG TGCCTTGGGG ATGAGGCTCC CTGCTCTCCC 
TTCTCCCCTT CTCCCAGCTA CAAGCTGTCT CCCTCTGGTT CCACACTATC CAACGTCGGC 
CTTGGAGCAA TCGGCTCAGG GCTCAGTCCC CAAAACTACG CTGCTAGACA AGGTGGCAAT 
CATGGTTTGT TTGGAAACAG CACAGCACAA TCGAGGGGCA TGCACACACC AGTGCAGCCA 
CTAAATCCTT CCCCCAATAT CCGGGCGCAA GTGCCTCCCC AATTTCTTTC TCCCCAGGTT 
TCGGCCTCCA TGCTCAAACA GTTCCCCAAC AGTGGCTTGA ATCCAGGTCT TTTCAATGTG 
GGGCCCCAGC TTTCTCCACA ACAGATTGCC ATGCTGAGCC AGCTTCCACA GATTCCCCAG 
TTTCAATTAG CATGTCAGCT CCTCCTTCAG CAGCAGCAGC AGCAGCAGCA GCTGTTACAG 
AGCCAGAGGA AGTTATCTCA AGCTGTGCGA CAGCAGCAGG AGCAACAGAT TGCTCGTATG 
GTGAGTGCCC TCCAGCAGCA GCAGCAGAGG CAGCCTGGCA TGAAGCATTC ACCATCCCAC 
CCTGTTGGGC CTAAGCCACA TATAGACAGC ATGGTACCCA ATCCTTTGAA TGTGGGTCTT 
TCAGATTTAC AGACCAAAGG ACAAATACCT GGATACGGTT CAGGCTTTGG CTCAAGCAGC 
ATGGATTATG GCATGGTGGG AGGGAAAGAA GCTGGAACAG AATCCCGCTT TAAACAGTGG 
ACTATGATGG AAGGGCTGCC CTCTGTGGCT TCACAAGATG CCAATATGCA CAAAAATGGT 
GCAATAGTGC CCCCTGGAAA GGCTCGCGGA GGATCGCCCT ACAACCAGTT CGACATAATT 
CAGGGCGACC CACTAGGTGG CCATGCAGGC CCTGCTGGTG ATAGTTGGTT ACCTGCCAAA 
TCTCCACCGA CAAACAAGAT CGGAAGCAAA TCCAGCAACG CCAGCTGGCC TCCAGAGTTC 
CAACCAGGAG TGCCATGGAA AGGTATACAA AACATTGACC CTGAATCTGA CCCCTACGTG 
ACCCCTGGAA GTGTGCTGGG GGGGACAGCC ACATCTCCCA TTGTAGATAC TGACCACCAA 
CTTCTGCGGG ACAACACCAC AGGGTCTAAT TCTTCCCTCA ACACCTCGCT GCCTTCACCT 
GGTGCCTGGC CCTACAGTGC CTCTGACAAT TCCTTCACCA ACGTTCATAG CACTTCAGCA 
AAATTCAGTG ATTACAAATC AACATGGTCC CCAGATCCCA TAGGACACAA TCCCACTCAT 
CTCTCCAACA AGATGTGGAA AAACCATATT TCCTCAAGGA ACACTACAGG GCTGCCTCGC 
CCGCCTCCTG GCCTGACCAA CCCCAAACCA TCATCTCCAT GGAACAGCAC AGCACCCCGA 
TCGGTCAGGG GCTGGGGGGC ACAGGACTCA AGGCTTGCCT CTGGTGAGAA GGATCATCTG 
ATAACGCCAT TGATCAGACC TGAGGAGTGT GCATTTTACA GCACTAATGG TTTGGAGGAG 
AGAAAGTTGC AGTTGCCTGC ATCTACTTGG AGTGATGGTA GCTCAGTTCG TCCTAGCTAC 
TGGCTGGTTC TTCACAATCT CACTCCACAG ATCGATGGGT CAACCTTGAG GACGATCTGC 
ATGCAGCATG GTCCACTGCT GACATTCCAT CTGAACCTGA CCCAGGGCAC CGCTCTCATC 
CGATACAACA CCAAACAGGA GGCGGCCAAG GCCCAGACTG CACTGCACAT GTGTGTGTTG 
GGAAACACTA CCATCCTTGC TGAGTTTGCC ACAGATGAAG AGGTTAGTCG CTTTTTGACA 
CAAGCTCAGC CCCCTACACC TGCAGCAACC CCAAGTGCAC CCACGGCAGG CTGGCAATCC 
CTGGAGACAG GACAGAACCA GACAGATCCA GTTGGACCTG CTTTGAATCT CTTTGGTGGG 
TCAACAGGGC TTGGGCAGTG GAGCAGTGGT GGCGGCGGCA GCAGTGGGGG TGATCTCACT 
GGCGCTTCGT TGTGGGGGCC CCCAAACTAT TCTTCAAGCT TGTGGGGGGT CCCAAGCGTA 
GAGGATCCAC ACAGAATGGG CAGCCCTGCT CCCTTACTAC CTGGAGACCT TCTGGGAGGA 
GGGTCGGATT CAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_015288794.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015144280.1 Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X5, mRNA.

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CloneID	OGa29593
Clone ID Related Accession (Same CDS sequence)	XM_015288794.2 , XM_015288794.1
Accession Version	XM_015288794.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	trinucleotide repeat-containing gene 6B protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455714.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA AGAAAAGGAA GAAGGAGGAT AAGAAAAAGA AGGAATCTGC TCAGAAGGTC 
ACAGATCAAA AAACCAAAGT GCCCGAAGTG ACGAAACCAA GTTTAAGCCA ACCAGTGGCT 
GCCAGCCCAA TTGGCAACTC TCCATCGCCA CCAGTCAATG GTGGCAACAA TGCCAAAAGG 
GTGGCAGTGC CGAACGGACA ACCGCCAAGC GCCGCCCGCT ACATGCCTCG GGAGGTGCCG 
CCGCGATTCC GTTGCCAGCA GGACCACAAA GTGTTACTGA AACGTGGGCA GCCCCCTCCA 
CCATCCTGTA TGCTCCTTGG GGGTGGAGCA GGGCCTCCTC CCCCTACAGC ACCAGGAGCA 
AACCCAAACA ACGCACAACC AGTGACAGGA GCACTGCTGC AGAGCGACAG TGGGACTGCA 
ACAGATTCAA CAATTGGAGG TGCTGCTGCT TCAAATTATG CAAATTCCAC TTGGGGTCCT 
GGAGCCTCCT CCAGCAGCGG CACCAACGCC AACCCAACTC ACATCTGGGA CAAGGTGATT 
GTAGACGGGT CTGACATGGA AGAGTGGCCT TGTATCGCCA GCAAAGATGC TGAGTCTTCT 
TCCGAAAACA CCACCGATAA CAACAGTGCC TCGAACCCTG GCTTGGAGAA GAACACTCTG 
CCAGGAAGCA CCACTAGTAA CAAAGGAAAA GGAAGCCAGT GCCAGTCTGG AAGCGCTGGA 
AATGAATGTA ATCTTGGGGC CTGGAAATCT GATCCCAAGG CTAAATCTGT TCAATCTTCC 
AACCCTGCTA CCGAGAGTAA CAGTGGACTA AGTAATTGGA GGAACTTGAC TGGACAAGAT 
AGAATTGGTT CTGGTTCTGG CTTCAGCAAC TTTAACCCAA ATAGCAACCC GTCTGCTTGG 
CCAGCACTGG TCCAAGAGGG CAATTCTAGG AAAGGGACTT CGGAATCTGA TAGTGGTAGC 
TCCAATGCAC AGATTAGCAC AGTAGGTCAG ACCTCTAGGG AACAGCAGTC AAAGATGGAA 
AATGCGGGTG TTAACTTTGT CTCTGGCAGA GAACAGGCTC AAATTCATAA CACTGATGGA 
CCAAAAAATG GAAACACTAA CTCCTTGAAC TTAAGTTCAC CAAACCCTAT GGAGAATAAG 
GGAATGCCCT TTGAAATGGG CTTGGGGAAC CCTTCCAGGA GCACTGACAC CCCTTCACAA 
AGCACTGGAG AAAGAAAGAC TGGGAGTGTT GGATCTTGGG GTACAGCTAG GGGGTCTTCT 
GGAACTGACA CAGTCTCTGG ACAGAGCAAT TCTGGAAACC ATGGGAACAA TGGAAAGGAT 
AGGGAGGACT CCTGGAAAGG AGCTTCTGTT CAAAAACCTA ATGGGTCAAG AAGCGATTCT 
TGGGATAACA ATAACCGGTC TACGGGTGGT GGGTCTTGGA ACTTTGGCCC TCAGCATGCA 
AATGAAAGCA AATGGGGTGA AGGGAACAAA TTAACATCTG GGGTCTCTCA GGGAGAATGG 
AAACAGCTGT CTGGGTCTGA TGAACTGAAG ATTGGAGAAT GGAGTGGTCC AAACCAACCA 
AATTCTAGCA CTGGAGCATG GGACAATCAA AAAGGCCACC CTCTTCCTGA AAACCAAGGC 
AATTCCCAGG CTCCCTGTTG GGGAAGATCT TCCAGCTCTG CAGGAAGTGA GGTTGGAGGT 
CAAAGCACTG GAAGCAACCA CAAAGCAGGA AGTAGTGACA GTCACAATTC TGGACGCCGA 
TCATACAGGC CTACGCATCC TGATTGCCAG GCAGTCTTGC AGACTTTGCT GAGCAGGACT 
GATTTGGACC CCCGAGTGCT TTCAAACACT GGCTGGGGCC AAACTCAAAT TAAGCAAGAT 
ACCGTATGGG ATATTGAAGA GATGCCACGG TCCGAGGGGA AGTCTGATAA AGGAACTGAG 
GGGTGGGAGA GCTCTGCCAC ACAGACAAAG AACTCAGGGG GCTGGGGCGA TGTACCCAGC 
CAAAGCAATC AAATCAAGTC TGGATGGGGT GAGCTCTCAA CCCCAACAGA ATGGAAGGAC 
CCCAAAAATA CTGGAGGATG GAATGACTAT AAGAACCCCA ATTCGTCTAA TTGGGGAGGG 
GGAAGACAAG AAGAGAAATC GTCATCTTCT TGGAATGACA GCTCTAGTAA GGATCAGGGG 
TGGGGTGGAC GGCAGCCTAA TCAAGGATGG TCTTCTGGAA AGAATGGTTG GGGAGAGGAA 
GTTGACCAAG CAAAAAACAG CAACTGGGAA AGTGCGGGAA ATAAGCCAGC ATCTGGTTGG 
GGTGAAGGTG GGCAAAATGA GATTGGAACT TGGGGTAACG GTGCAAATAC AGGCTCTGCT 
TCAAAGGGTG GTTGGGATGA TTGTAAAAGA AATTCAACGT GGAACGAGAC GGGTCGACAG 
CCCAACTCCT GGAACAAGCA GCAGCAGCAG CAACAGCAGC AACAGCAAGA GACTTCTGGT 
TCCTGGGGTC CACCGCCACA AACTCCAAGT AATGGGCGAC CTCCAAACCC AAACTGGAAC 
AGCGGACCAC AACCAGCTGC CCCCAAAGAC GAGGAGCCTA GTGGCTGGGA AGAACCTTCT 
CCACAGTCAA TCAGTCGAAA AATGGATATT GATGATGGCA CTTCAGCGTG GGGAGATCCT 
AGCAGTTATA ACTACAAGAA TGTGAACCTG TGGGACAAGA ACTCACAAGG GGGACAGGCC 
CCACGGGAAC AGAGCCTGCC TACTCCAATG ACTAGCAAAT CACAAGCATC AGTCTGGAGC 
AAAAGCACTC CACCTGCTCC AGATAATGGT ACGTCCGCCT GGGGTGAGCC AAATGAAACC 
AGTCCCGGGT GGGGTGAAGT AGATGATACA GGAGCATCGA CAACAGGCTG GGGGAATGCA 
CCTTCCAACG CCCCGAATGC CATGAAATCT AGTTCTAAAT CTATGCAAGA TGGCTGGGGA 
GAGAGTGATG GGCCAGTGAC AGGAACACGT CATTCCAGCT GGGAAGAGGA GGAGGAGGGA 
GGAGTCTGGA ACACAGCAGG CTCTCAGGGA AGCAGTTCCT CCCACAACTC AACAAGCTGG 
GGGCAAGGAG GAAAGAAAAC GCAGATGAAG TGCTCGTTAA AAGGAGGAAA TAGTGATTCA 
TGGATGAACC CTATATCCAA GCAGTTTTCA AATATGGGCT TGCTAAGTCA AACTGAGGAC 
ATTCCAGGCA ATAAGATGGA CTTGTCTGTA GGTGGTCTCC CAGATAAGAA GTTTGAGGTA 
GATAAACGAG CCATGAATCT CGGGGATTTT AATGACATAA TGAGAAAGGA TCGATCTGGG 
TTCCGTCCAC CTAATTCCAA AGACATGGGA ACCACAGATA GTGGGCCTTA TTTTGAGAAG 
CTGACTTTGC CTTTCTCCAA TCAAGATGGG TGCCTTGGGG ATGAGGCTCC CTGCTCTCCC 
TTCTCCCCTT CTCCCAGCTA CAAGCTGTCT CCCTCTGGTT CCACACTATC CAACGTCGGC 
CTTGGAGCAA TCGGCTCAGG GCTCAGTCCC CAAAACTACG CTGCTAGACA AGGTGGCAAT 
CATGGTTTGT TTGGAAACAG CACAGCACAA TCGAGGGGCA TGCACACACC AGTGCAGCCA 
CTAAATCCTT CCCCCAATAT CCGGGCGCAA GTGCCTCCCC AATTTCTTTC TCCCCAGGTT 
TCGGCCTCCA TGCTCAAACA GTTCCCCAAC AGTGGCTTGA ATCCAGGTCT TTTCAATGTG 
GGGCCCCAGC TTTCTCCACA ACAGATTGCC ATGCTGAGCC AGCTTCCACA GATTCCCCAG 
TTTCAATTAG CATGTCAGCT CCTCCTTCAG CAGCAGCAGC AGCAGCAGCA GCTGTTACAG 
AGCCAGAGGA AGTTATCTCA AGCTGTGCGA CAGCAGCAGG AGCAACAGAT TGCTCGTATG 
GTGAGTGCCC TCCAGCAGCA GCAGCAGAGG CAGCCTGGCA TGAAGCATTC ACCATCCCAC 
CCTGTTGGGC CTAAGCCACA TATAGACAGC ATGGTACCCA ATCCTTTGAA TGTGGGTCTT 
TCAGATTTAC AGACCAAAGG ACAAATACCT GGATACGGTT CAGGCTTTGG CTCAAGCAGC 
ATGGATTATG GCATGGTGGG AGGGAAAGAA GCTGGAACAG AATCCCGCTT TAAACAGTGG 
ACTATGATGG AAGGGCTGCC CTCTGTGGCT TCACAAGATG CCAATATGCA CAAAAATGGT 
GCAATAGTGC CCCCTGGAAA GGCTCGCGGA GGATCGCCCT ACAACCAGTT CGACATAATT 
CAGGGCGACC CACTAGGTGG CCATGCAGGC CCTGCTGGTG ATAGTTGGTT ACCTGCCAAA 
TCTCCACCGA CAAACAAGAT CGGAAGCAAA TCCAGCAACG CCAGCTGGCC TCCAGAGTTC 
CAACCAGGAG TGCCATGGAA AGGTATACAA AACATTGACC CTGAATCTGA CCCCTACGTG 
ACCCCTGGAA GTGTGCTGGG GGGGACAGCC ACATCTCCCA TTGTAGATAC TGACCACCAA 
CTTCTGCGGG ACAACACCAC AGGGTCTAAT TCTTCCCTCA ACACCTCGCT GCCTTCACCT 
GGTGCCTGGC CCTACAGTGC CTCTGACAAT TCCTTCACCA ACGTTCATAG CACTTCAGCA 
AAATTCAGTG ATTACAAATC AACATGGTCC CCAGATCCCA TAGGACACAA TCCCACTCAT 
CTCTCCAACA AGATGTGGAA AAACCATATT TCCTCAAGGA ACACTACAGG GCTGCCTCGC 
CCGCCTCCTG GCCTGACCAA CCCCAAACCA TCATCTCCAT GGAACAGCAC AGCACCCCGA 
TCGGTCAGGG GCTGGGGGGC ACAGGACTCA AGGCTTGCCT CTGGTGAGAA GGATCATCTG 
ATAACGCCAT TGATCAGACC TGAGGAGTGT GCATTTTACA GCACTAATGG TTTGGAGGAG 
AGAAAGTTGC AGTTGCCTGC ATCTACTTGG AGTGATGGTA GCTCAGTTCG TCCTAGCTAC 
TGGCTGGTTC TTCACAATCT CACTCCACAG ATCGATGGGT CAACCTTGAG GACGATCTGC 
ATGCAGCATG GTCCACTGCT GACATTCCAT CTGAACCTGA CCCAGGGCAC CGCTCTCATC 
CGATACAACA CCAAACAGGA GGCGGCCAAG GCCCAGACTG CACTGCACAT GTGTGTGTTG 
GGAAACACTA CCATCCTTGC TGAGTTTGCC ACAGATGAAG AGGTTAGTCG CTTTTTGACA 
CAAGCTCAGC CCCCTACACC TGCAGCAACC CCAAGTGCAC CCACGGCAGG CTGGCAATCC 
CTGGAGACAG GACAGAACCA GACAGATCCA GTTGGACCTG CTTTGAATCT CTTTGGTGGG 
TCAACAGGGC TTGGGCAGTG GAGCAGTGGT GGCGGCGGCA GCAGTGGGGG TGATCTCACT 
GGCGCTTCGT TGTGGGGGCC CCCAAACTAT TCTTCAAGCT TGTGGGGGGT CCCAAGCGTA 
GAGGATCCAC ACAGAATGGG CAGCCCTGCT CCCTTACTAC CTGGAGACCT TCTGGGAGGA 
GGGTCGGATT CAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015144280.1
CDS	389..5926
Translation	MEDKKRKKEDKKKKESAQKVTDQKTKVPEVTKPSLSQPVAASPIGNSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPPTAPGANPNNAQPVTGALLQSDSGTATDSTIGGAAASNYANSTWGPGASSSSGTNANPTHIWDKVIVDGSDMEEWPCIASKDAESSSENTTDNNSASNPGLEKNTLPGSTTSNKGKGSQCQSGSAGNECNLGAWKSDPKAKSVQSSNPATESNSGLSNWRNLTGQDRIGSGSGFSNFNPNSNPSAWPALVQEGNSRKGTSESDSGSSNAQISTVGQTSREQQSKMENAGVNFVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFEMGLGNPSRSTDTPSQSTGERKTGSVGSWGTARGSSGTDTVSGQSNSGNHGNNGKDREDSWKGASVQKPNGSRSDSWDNNNRSTGGGSWNFGPQHANESKWGEGNKLTSGVSQGEWKQLSGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNSQAPCWGRSSSSAGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEMPRSEGKSDKGTEGWESSATQTKNSGGWGDVPSQSNQIKSGWGELSTPTEWKDPKNTGGWNDYKNPNSSNWGGGRQEEKSSSSWNDSSSKDQGWGGRQPNQGWSSGKNGWGEEVDQAKNSNWESAGNKPASGWGEGGQNEIGTWGNGANTGSASKGGWDDCKRNSTWNETGRQPNSWNKQQQQQQQQQQETSGSWGPPPQTPSNGRPPNPNWNSGPQPAAPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPSSYNYKNVNLWDKNSQGGQAPREQSLPTPMTSKSQASVWSKSTPPAPDNGTSAWGEPNETSPGWGEVDDTGASTTGWGNAPSNAPNAMKSSSKSMQDGWGESDGPVTGTRHSSWEEEEEGGVWNTAGSQGSSSSHNSTSWGQGGKKTQMKCSLKGGNSDSWMNPISKQFSNMGLLSQTEDIPGNKMDLSVGGLPDKKFEVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLSNVGLGAIGSGLSPQNYAARQGGNHGLFGNSTAQSRGMHTPVQPLNPSPNIRAQVPPQFLSPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQQLLQSQRKLSQAVRQQQEQQIARMVSALQQQQQRQPGMKHSPSHPVGPKPHIDSMVPNPLNVGLSDLQTKGQIPGYGSGFGSSSMDYGMVGGKEAGTESRFKQWTMMEGLPSVASQDANMHKNGAIVPPGKARGGSPYNQFDIIQGDPLGGHAGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFSDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTGLPRPPPGLTNPKPSSPWNSTAPRSVRGWGAQDSRLASGEKDHLITPLIRPEECAFYSTNGLEERKLQLPASTWSDGSSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYNTKQEAAKAQTALHMCVLGNTTILAEFATDEEVSRFLTQAQPPTPAATPSAPTAGWQSLETGQNQTDPVGPALNLFGGSTGLGQWSSGGGGSSGGDLTGASLWGPPNYSSSLWGVPSVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_015288794.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015288794.1

ORF Insert Sequence:

ATGGAAGACA AGAAAAGGAA GAAGGAGGAT AAGAAAAAGA AGGAATCTGC TCAGAAGGTC 
ACAGATCAAA AAACCAAAGT GCCCGAAGTG ACGAAACCAA GTTTAAGCCA ACCAGTGGCT 
GCCAGCCCAA TTGGCAACTC TCCATCGCCA CCAGTCAATG GTGGCAACAA TGCCAAAAGG 
GTGGCAGTGC CGAACGGACA ACCGCCAAGC GCCGCCCGCT ACATGCCTCG GGAGGTGCCG 
CCGCGATTCC GTTGCCAGCA GGACCACAAA GTGTTACTGA AACGTGGGCA GCCCCCTCCA 
CCATCCTGTA TGCTCCTTGG GGGTGGAGCA GGGCCTCCTC CCCCTACAGC ACCAGGAGCA 
AACCCAAACA ACGCACAACC AGTGACAGGA GCACTGCTGC AGAGCGACAG TGGGACTGCA 
ACAGATTCAA CAATTGGAGG TGCTGCTGCT TCAAATTATG CAAATTCCAC TTGGGGTCCT 
GGAGCCTCCT CCAGCAGCGG CACCAACGCC AACCCAACTC ACATCTGGGA CAAGGTGATT 
GTAGACGGGT CTGACATGGA AGAGTGGCCT TGTATCGCCA GCAAAGATGC TGAGTCTTCT 
TCCGAAAACA CCACCGATAA CAACAGTGCC TCGAACCCTG GCTTGGAGAA GAACACTCTG 
CCAGGAAGCA CCACTAGTAA CAAAGGAAAA GGAAGCCAGT GCCAGTCTGG AAGCGCTGGA 
AATGAATGTA ATCTTGGGGC CTGGAAATCT GATCCCAAGG CTAAATCTGT TCAATCTTCC 
AACCCTGCTA CCGAGAGTAA CAGTGGACTA AGTAATTGGA GGAACTTGAC TGGACAAGAT 
AGAATTGGTT CTGGTTCTGG CTTCAGCAAC TTTAACCCAA ATAGCAACCC GTCTGCTTGG 
CCAGCACTGG TCCAAGAGGG CAATTCTAGG AAAGGGACTT CGGAATCTGA TAGTGGTAGC 
TCCAATGCAC AGATTAGCAC AGTAGGTCAG ACCTCTAGGG AACAGCAGTC AAAGATGGAA 
AATGCGGGTG TTAACTTTGT CTCTGGCAGA GAACAGGCTC AAATTCATAA CACTGATGGA 
CCAAAAAATG GAAACACTAA CTCCTTGAAC TTAAGTTCAC CAAACCCTAT GGAGAATAAG 
GGAATGCCCT TTGAAATGGG CTTGGGGAAC CCTTCCAGGA GCACTGACAC CCCTTCACAA 
AGCACTGGAG AAAGAAAGAC TGGGAGTGTT GGATCTTGGG GTACAGCTAG GGGGTCTTCT 
GGAACTGACA CAGTCTCTGG ACAGAGCAAT TCTGGAAACC ATGGGAACAA TGGAAAGGAT 
AGGGAGGACT CCTGGAAAGG AGCTTCTGTT CAAAAACCTA ATGGGTCAAG AAGCGATTCT 
TGGGATAACA ATAACCGGTC TACGGGTGGT GGGTCTTGGA ACTTTGGCCC TCAGCATGCA 
AATGAAAGCA AATGGGGTGA AGGGAACAAA TTAACATCTG GGGTCTCTCA GGGAGAATGG 
AAACAGCTGT CTGGGTCTGA TGAACTGAAG ATTGGAGAAT GGAGTGGTCC AAACCAACCA 
AATTCTAGCA CTGGAGCATG GGACAATCAA AAAGGCCACC CTCTTCCTGA AAACCAAGGC 
AATTCCCAGG CTCCCTGTTG GGGAAGATCT TCCAGCTCTG CAGGAAGTGA GGTTGGAGGT 
CAAAGCACTG GAAGCAACCA CAAAGCAGGA AGTAGTGACA GTCACAATTC TGGACGCCGA 
TCATACAGGC CTACGCATCC TGATTGCCAG GCAGTCTTGC AGACTTTGCT GAGCAGGACT 
GATTTGGACC CCCGAGTGCT TTCAAACACT GGCTGGGGCC AAACTCAAAT TAAGCAAGAT 
ACCGTATGGG ATATTGAAGA GATGCCACGG TCCGAGGGGA AGTCTGATAA AGGAACTGAG 
GGGTGGGAGA GCTCTGCCAC ACAGACAAAG AACTCAGGGG GCTGGGGCGA TGTACCCAGC 
CAAAGCAATC AAATCAAGTC TGGATGGGGT GAGCTCTCAA CCCCAACAGA ATGGAAGGAC 
CCCAAAAATA CTGGAGGATG GAATGACTAT AAGAACCCCA ATTCGTCTAA TTGGGGAGGG 
GGAAGACAAG AAGAGAAATC GTCATCTTCT TGGAATGACA GCTCTAGTAA GGATCAGGGG 
TGGGGTGGAC GGCAGCCTAA TCAAGGATGG TCTTCTGGAA AGAATGGTTG GGGAGAGGAA 
GTTGACCAAG CAAAAAACAG CAACTGGGAA AGTGCGGGAA ATAAGCCAGC ATCTGGTTGG 
GGTGAAGGTG GGCAAAATGA GATTGGAACT TGGGGTAACG GTGCAAATAC AGGCTCTGCT 
TCAAAGGGTG GTTGGGATGA TTGTAAAAGA AATTCAACGT GGAACGAGAC GGGTCGACAG 
CCCAACTCCT GGAACAAGCA GCAGCAGCAG CAACAGCAGC AACAGCAAGA GACTTCTGGT 
TCCTGGGGTC CACCGCCACA AACTCCAAGT AATGGGCGAC CTCCAAACCC AAACTGGAAC 
AGCGGACCAC AACCAGCTGC CCCCAAAGAC GAGGAGCCTA GTGGCTGGGA AGAACCTTCT 
CCACAGTCAA TCAGTCGAAA AATGGATATT GATGATGGCA CTTCAGCGTG GGGAGATCCT 
AGCAGTTATA ACTACAAGAA TGTGAACCTG TGGGACAAGA ACTCACAAGG GGGACAGGCC 
CCACGGGAAC AGAGCCTGCC TACTCCAATG ACTAGCAAAT CACAAGCATC AGTCTGGAGC 
AAAAGCACTC CACCTGCTCC AGATAATGGT ACGTCCGCCT GGGGTGAGCC AAATGAAACC 
AGTCCCGGGT GGGGTGAAGT AGATGATACA GGAGCATCGA CAACAGGCTG GGGGAATGCA 
CCTTCCAACG CCCCGAATGC CATGAAATCT AGTTCTAAAT CTATGCAAGA TGGCTGGGGA 
GAGAGTGATG GGCCAGTGAC AGGAACACGT CATTCCAGCT GGGAAGAGGA GGAGGAGGGA 
GGAGTCTGGA ACACAGCAGG CTCTCAGGGA AGCAGTTCCT CCCACAACTC AACAAGCTGG 
GGGCAAGGAG GAAAGAAAAC GCAGATGAAG TGCTCGTTAA AAGGAGGAAA TAGTGATTCA 
TGGATGAACC CTATATCCAA GCAGTTTTCA AATATGGGCT TGCTAAGTCA AACTGAGGAC 
ATTCCAGGCA ATAAGATGGA CTTGTCTGTA GGTGGTCTCC CAGATAAGAA GTTTGAGGTA 
GATAAACGAG CCATGAATCT CGGGGATTTT AATGACATAA TGAGAAAGGA TCGATCTGGG 
TTCCGTCCAC CTAATTCCAA AGACATGGGA ACCACAGATA GTGGGCCTTA TTTTGAGAAG 
CTGACTTTGC CTTTCTCCAA TCAAGATGGG TGCCTTGGGG ATGAGGCTCC CTGCTCTCCC 
TTCTCCCCTT CTCCCAGCTA CAAGCTGTCT CCCTCTGGTT CCACACTATC CAACGTCGGC 
CTTGGAGCAA TCGGCTCAGG GCTCAGTCCC CAAAACTACG CTGCTAGACA AGGTGGCAAT 
CATGGTTTGT TTGGAAACAG CACAGCACAA TCGAGGGGCA TGCACACACC AGTGCAGCCA 
CTAAATCCTT CCCCCAATAT CCGGGCGCAA GTGCCTCCCC AATTTCTTTC TCCCCAGGTT 
TCGGCCTCCA TGCTCAAACA GTTCCCCAAC AGTGGCTTGA ATCCAGGTCT TTTCAATGTG 
GGGCCCCAGC TTTCTCCACA ACAGATTGCC ATGCTGAGCC AGCTTCCACA GATTCCCCAG 
TTTCAATTAG CATGTCAGCT CCTCCTTCAG CAGCAGCAGC AGCAGCAGCA GCTGTTACAG 
AGCCAGAGGA AGTTATCTCA AGCTGTGCGA CAGCAGCAGG AGCAACAGAT TGCTCGTATG 
GTGAGTGCCC TCCAGCAGCA GCAGCAGAGG CAGCCTGGCA TGAAGCATTC ACCATCCCAC 
CCTGTTGGGC CTAAGCCACA TATAGACAGC ATGGTACCCA ATCCTTTGAA TGTGGGTCTT 
TCAGATTTAC AGACCAAAGG ACAAATACCT GGATACGGTT CAGGCTTTGG CTCAAGCAGC 
ATGGATTATG GCATGGTGGG AGGGAAAGAA GCTGGAACAG AATCCCGCTT TAAACAGTGG 
ACTATGATGG AAGGGCTGCC CTCTGTGGCT TCACAAGATG CCAATATGCA CAAAAATGGT 
GCAATAGTGC CCCCTGGAAA GGCTCGCGGA GGATCGCCCT ACAACCAGTT CGACATAATT 
CAGGGCGACC CACTAGGTGG CCATGCAGGC CCTGCTGGTG ATAGTTGGTT ACCTGCCAAA 
TCTCCACCGA CAAACAAGAT CGGAAGCAAA TCCAGCAACG CCAGCTGGCC TCCAGAGTTC 
CAACCAGGAG TGCCATGGAA AGGTATACAA AACATTGACC CTGAATCTGA CCCCTACGTG 
ACCCCTGGAA GTGTGCTGGG GGGGACAGCC ACATCTCCCA TTGTAGATAC TGACCACCAA 
CTTCTGCGGG ACAACACCAC AGGGTCTAAT TCTTCCCTCA ACACCTCGCT GCCTTCACCT 
GGTGCCTGGC CCTACAGTGC CTCTGACAAT TCCTTCACCA ACGTTCATAG CACTTCAGCA 
AAATTCAGTG ATTACAAATC AACATGGTCC CCAGATCCCA TAGGACACAA TCCCACTCAT 
CTCTCCAACA AGATGTGGAA AAACCATATT TCCTCAAGGA ACACTACAGG GCTGCCTCGC 
CCGCCTCCTG GCCTGACCAA CCCCAAACCA TCATCTCCAT GGAACAGCAC AGCACCCCGA 
TCGGTCAGGG GCTGGGGGGC ACAGGACTCA AGGCTTGCCT CTGGTGAGAA GGATCATCTG 
ATAACGCCAT TGATCAGACC TGAGGAGTGT GCATTTTACA GCACTAATGG TTTGGAGGAG 
AGAAAGTTGC AGTTGCCTGC ATCTACTTGG AGTGATGGTA GCTCAGTTCG TCCTAGCTAC 
TGGCTGGTTC TTCACAATCT CACTCCACAG ATCGATGGGT CAACCTTGAG GACGATCTGC 
ATGCAGCATG GTCCACTGCT GACATTCCAT CTGAACCTGA CCCAGGGCAC CGCTCTCATC 
CGATACAACA CCAAACAGGA GGCGGCCAAG GCCCAGACTG CACTGCACAT GTGTGTGTTG 
GGAAACACTA CCATCCTTGC TGAGTTTGCC ACAGATGAAG AGGTTAGTCG CTTTTTGACA 
CAAGCTCAGC CCCCTACACC TGCAGCAACC CCAAGTGCAC CCACGGCAGG CTGGCAATCC 
CTGGAGACAG GACAGAACCA GACAGATCCA GTTGGACCTG CTTTGAATCT CTTTGGTGGG 
TCAACAGGGC TTGGGCAGTG GAGCAGTGGT GGCGGCGGCA GCAGTGGGGG TGATCTCACT 
GGCGCTTCGT TGTGGGGGCC CCCAAACTAT TCTTCAAGCT TGTGGGGGGT CCCAAGCGTA 
GAGGATCCAC ACAGAATGGG CAGCCCTGCT CCCTTACTAC CTGGAGACCT TCTGGGAGGA 
GGGTCGGATT CAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_015288799.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015144285.1 Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X5, mRNA.

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CloneID	OGa29594
Clone ID Related Accession (Same CDS sequence)	XM_015288799.2 , XM_015288799.1
Accession Version	XM_015288799.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	trinucleotide repeat-containing gene 6B protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288799.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACTG ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGGTCAG TGAGATGGAA 
CATGAGGATT TTGTAATGGA GGGGCATGGC AAGACTCCAC CTCCTGGTGA AGAAAACAAA 
CAAGAGAAGG AGCAAGAAAG GGAAGAACAG TTAATGGAAG ACAAGAAAAG GAAGAAGGAG 
GATAAGAAAA AGAAGGAATC TGCTCAGAAG GTCACAGATC AAAAAACCAA AGTGCCCGAA 
GTGACGAAAC CAAGTTTAAG CCAACCAGTG GCTGCCAGCC CAATTGGCAA CTCTCCATCG 
CCACCAGTCA ATGGTGGCAA CAATGCCAAA AGGGTGGCAG TGCCGAACGG ACAACCGCCA 
AGCGCCGCCC GCTACATGCC TCGGGAGGTG CCGCCGCGAT TCCGTTGCCA GCAGGACCAC 
AAAGTGTTAC TGAAACGTGG GCAGCCCCCT CCACCATCCT GTATGCTCCT TGGGGGTGGA 
GCAGGGCCTC CTCCCCCTAC AGCACCAGGA GCAAACCCAA ACAACGCACA ACCAGTGACA 
GGAGCACTGC TGCAGAGCGA CAGTGGGACT GCAACAGATT CAACAATTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGT CCTGGAGCCT CCTCCAGCAG CGGCACCAAC 
GCCAACCCAA CTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGTATCG CCAGCAAAGA TGCTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCGAACC CTGGCTTGGA GAAGAACACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGAAGCC AGTGCCAGTC TGGAAGCGCT GGAAATGAAT GTAATCTTGG GGCCTGGAAA 
TCTGATCCCA AGGCTAAATC TGTTCAATCT TCCAACCCTG CTACCGAGAG TAACAGTGGA 
CTAAGTAATT GGAGGAACTT GACTGGACAA GATAGAATTG GTTCTGGTTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCGTCTGCT TGGCCAGCAC TGGTCCAAGA GGGCAATTCT 
AGGAAAGGGA CTTCGGAATC TGATAGTGGT AGCTCCAATG CACAGATTAG CACAGTAGGT 
CAGACCTCTA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAACTT TGTCTCTGGC 
AGAGAACAGG CTCAAATTCA TAACACTGAT GGACCAAAAA ATGGAAACAC TAACTCCTTG 
AACTTAAGTT CACCAAACCC TATGGAGAAT AAGGGAATGC CCTTTGAAAT GGGCTTGGGG 
AACCCTTCCA GGAGCACTGA CACCCCTTCA CAAAGCACTG GAGAAAGAAA GACTGGGAGT 
GTTGGATCTT GGGGTACAGC TAGGGGGTCT TCTGGAACTG ACACAGTCTC TGGACAGAGC 
AATTCTGGAA ACCATGGGAA CAATGGAAAG GATAGGGAGG ACTCCTGGAA AGGAGCTTCT 
GTTCAAAAAC CTAATGGGTC AAGAAGCGAT TCTTGGGATA ACAATAACCG GTCTACGGGT 
GGTGGGTCTT GGAACTTTGG CCCTCAGCAT GCAAATGAAA GCAAATGGGG TGAAGGGAAC 
AAATTAACAT CTGGGGTCTC TCAGGGAGAA TGGAAACAGC TGTCTGGGTC TGATGAACTG 
AAGATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAAAAAGGCC ACCCTCTTCC TGAAAACCAA GGCAATTCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CTGCAGGAAG TGAGGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGACGC CGATCATACA GGCCTACGCA TCCTGATTGC 
CAGGCAGTCT TGCAGACTTT GCTGAGCAGG ACTGATTTGG ACCCCCGAGT GCTTTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAA GATACCGTAT GGGATATTGA AGAGATGCCA 
CGGTCCGAGG GGAAGTCTGA TAAAGGAACT GAGGGGTGGG AGAGCTCTGC CACACAGACA 
AAGAACTCAG GGGGCTGGGG CGATGTACCC AGCCAAAGCA ATCAAATCAA GTCTGGATGG 
GGTGAGCTCT CAACCCCAAC AGAATGGAAG GACCCCAAAA ATACTGGAGG ATGGAATGAC 
TATAAGAACC CCAATTCGTC TAATTGGGGA GGGGGAAGAC AAGAAGAGAA ATCGTCATCT 
TCTTGGAATG ACAGCTCTAG TAAGGATCAG GGGTGGGGTG GACGGCAGCC TAATCAAGGA 
TGGTCTTCTG GAAAGAATGG TTGGGGAGAG GAAGTTGACC AAGCAAAAAA CAGCAACTGG 
GAAAGTGCGG GAAATAAGCC AGCATCTGGT TGGGGTGAAG GTGGGCAAAA TGAGATTGGA 
ACTTGGGGTA ACGGTGCAAA TACAGGCTCT GCTTCAAAGG GTGGTTGGGA TGATTGTAAA 
AGAAATTCAA CGTGGAACGA GACGGGTCGA CAGCCCAACT CCTGGAACAA GCAGCAGCAG 
CAGCAACAGC AGCAACAGCA AGAGACTTCT GGTTCCTGGG GTCCACCGCC ACAAACTCCA 
AGTAATGGGC GACCTCCAAA CCCAAACTGG AACAGCGGAC CACAACCAGC TGCCCCCAAA 
GACGAGGAGC CTAGTGGCTG GGAAGAACCT TCTCCACAGT CAATCAGTCG AAAAATGGAT 
ATTGATGATG GCACTTCAGC GTGGGGAGAT CCTAGCAGTT ATAACTACAA GAATGTGAAC 
CTGTGGGACA AGAACTCACA AGGGGGACAG GCCCCACGGG AACAGAGCCT GCCTACTCCA 
ATGACTAGCA AATCACAAGC ATCAGTCTGG AGCAAAAGCA CTCCACCTGC TCCAGATAAT 
GGTACGTCCG CCTGGGGTGA GCCAAATGAA ACCAGTCCCG GGTGGGGTGA AGTAGATGAT 
ACAGGAGCAT CGACAACAGG CTGGGGGAAT GCACCTTCCA ACGCCCCGAA TGCCATGAAA 
TCTAGTTCTA AATCTATGCA AGATGGCTGG GGAGAGAGTG ATGGGCCAGT GACAGGAACA 
CGTCATTCCA GCTGGGAAGA GGAGGAGGAG GGAGGAGTCT GGAACACAGC AGGCTCTCAG 
GGAAGCAGTT CCTCCCACAA CTCAACAAGC TGGGGGCAAG GAGGAAAGAA AACGCAGATG 
AAGTGCTCGT TAAAAGGAGG AAATAGTGAT TCATGGATGA ACCCTATATC CAAGCAGTTT 
TCAAATATGG GCTTGCTAAG TCAAACTGAG GACATTCCAG GCAATAAGAT GGACTTGTCT 
GTAGGTGGTC TCCCAGATAA GAAGTTTGAG GTAGATAAAC GAGCCATGAA TCTCGGGGAT 
TTTAATGACA TAATGAGAAA GGATCGATCT GGGTTCCGTC CACCTAATTC CAAAGACATG 
GGAACCACAG ATAGTGGGCC TTATTTTGAG AAGGGTGGCA ATCATGGTTT GTTTGGAAAC 
AGCACAGCAC AATCGAGGGG CATGCACACA CCAGTGCAGC CACTAAATCC TTCCCCCAAT 
ATCCGGGCGC AAGTGCCTCC CCAATTTCTT TCTCCCCAGG TTTCGGCCTC CATGCTCAAA 
CAGTTCCCCA ACAGTGGCTT GAATCCAGGT CTTTTCAATG TGGGGCCCCA GCTTTCTCCA 
CAACAGATTG CCATGCTGAG CCAGCTTCCA CAGATTCCCC AGTTTCAATT AGCATGTCAG 
CTCCTCCTTC AGCAGCAGCA GCAGCAGCAG CAGCTGTTAC AGAGCCAGAG GAAGTTATCT 
CAAGCTGTGC GACAGCAGCA GGAGCAACAG ATTGCTCGTA TGGTGAGTGC CCTCCAGCAG 
CAGCAGCAGA GGCAGCCTGG CATGAAGCAT TCACCATCCC ACCCTGTTGG GCCTAAGCCA 
CATATAGACA GCATGGTACC CAATCCTTTG AATGTGGGTC TTTCAGATTT ACAGACCAAA 
GGACAAATAC CTGGATACGG TTCAGGCTTT GGCTCAAGCA GCATGGATTA TGGCATGGTG 
GGAGGGAAAG AAGCTGGAAC AGAATCCCGC TTTAAACAGT GGACTATGAT GGAAGGGCTG 
CCCTCTGTGG CTTCACAAGA TGCCAATATG CACAAAAATG GTGCAATAGT GCCCCCTGGA 
AAGGCTCGCG GAGGATCGCC CTACAACCAG TTCGACATAA TTCAGGGCGA CCCACTAGGT 
GGCCATGCAG GCCCTGCTGG TGATAGTTGG TTACCTGCCA AATCTCCACC GACAAACAAG 
ATCGGAAGCA AATCCAGCAA CGCCAGCTGG CCTCCAGAGT TCCAACCAGG AGTGCCATGG 
AAAGGTATAC AAAACATTGA CCCTGAATCT GACCCCTACG TGACCCCTGG AAGTGTGCTG 
GGGGGGACAG CCACATCTCC CATTGTAGAT ACTGACCACC AACTTCTGCG GGACAACACC 
ACAGGGTCTA ATTCTTCCCT CAACACCTCG CTGCCTTCAC CTGGTGCCTG GCCCTACAGT 
GCCTCTGACA ATTCCTTCAC CAACGTTCAT AGCACTTCAG CAAAATTCAG TGATTACAAA 
TCAACATGGT CCCCAGATCC CATAGGACAC AATCCCACTC ATCTCTCCAA CAAGATGTGG 
AAAAACCATA TTTCCTCAAG GAACACTACA GGGCTGCCTC GCCCGCCTCC TGGCCTGACC 
AACCCCAAAC CATCATCTCC ATGGAACAGC ACAGCACCCC GATCGGTCAG GGGCTGGGGG 
GCACAGGACT CAAGGCTTGC CTCTGGTGAG AAGGATCATC TGATAACGCC ATTGATCAGA 
CCTGAGGAGT GTGCATTTTA CAGCACTAAT GGTTTGGAGG AGAGAAAGTT GCAGTTGCCT 
GCATCTACTT GGAGTGATGG TAGCTCAGTT CGTCCTAGCT ACTGGCTGGT TCTTCACAAT 
CTCACTCCAC AGATCGATGG GTCAACCTTG AGGACGATCT GCATGCAGCA TGGTCCACTG 
CTGACATTCC ATCTGAACCT GACCCAGGGC ACCGCTCTCA TCCGATACAA CACCAAACAG 
GAGGCGGCCA AGGCCCAGAC TGCACTGCAC ATGTGTGTGT TGGGAAACAC TACCATCCTT 
GCTGAGTTTG CCACAGATGA AGAGGTTAGT CGCTTTTTGA CACAAGCTCA GCCCCCTACA 
CCTGCAGCAA CCCCAAGTGC ACCCACGGCA GGCTGGCAAT CCCTGGAGAC AGGACAGAAC 
CAGACAGATC CAGTTGGACC TGCTTTGAAT CTCTTTGGTG GGTCAACAGG GCTTGGGCAG 
TGGAGCAGTG GTGGCGGCGG CAGCAGTGGG GGTGATCTCA CTGGCGCTTC GTTGTGGGGG 
CCCCCAAACT ATTCTTCAAG CTTGTGGGGG GTCCCAAGCG TAGAGGATCC ACACAGAATG 
GGCAGCCCTG CTCCCTTACT ACCTGGAGAC CTTCTGGGAG GAGGGTCGGA TTCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015144285.1
CDS	548..6067
Translation	MQTDEGEILEEGSPKVSEMEHEDFVMEGHGKTPPPGEENKQEKEQEREEQLMEDKKRKKEDKKKKESAQKVTDQKTKVPEVTKPSLSQPVAASPIGNSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPPTAPGANPNNAQPVTGALLQSDSGTATDSTIGGAAASNYANSTWGPGASSSSGTNANPTHIWDKVIVDGSDMEEWPCIASKDAESSSENTTDNNSASNPGLEKNTLPGSTTSNKGKGSQCQSGSAGNECNLGAWKSDPKAKSVQSSNPATESNSGLSNWRNLTGQDRIGSGSGFSNFNPNSNPSAWPALVQEGNSRKGTSESDSGSSNAQISTVGQTSREQQSKMENAGVNFVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFEMGLGNPSRSTDTPSQSTGERKTGSVGSWGTARGSSGTDTVSGQSNSGNHGNNGKDREDSWKGASVQKPNGSRSDSWDNNNRSTGGGSWNFGPQHANESKWGEGNKLTSGVSQGEWKQLSGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNSQAPCWGRSSSSAGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEMPRSEGKSDKGTEGWESSATQTKNSGGWGDVPSQSNQIKSGWGELSTPTEWKDPKNTGGWNDYKNPNSSNWGGGRQEEKSSSSWNDSSSKDQGWGGRQPNQGWSSGKNGWGEEVDQAKNSNWESAGNKPASGWGEGGQNEIGTWGNGANTGSASKGGWDDCKRNSTWNETGRQPNSWNKQQQQQQQQQQETSGSWGPPPQTPSNGRPPNPNWNSGPQPAAPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPSSYNYKNVNLWDKNSQGGQAPREQSLPTPMTSKSQASVWSKSTPPAPDNGTSAWGEPNETSPGWGEVDDTGASTTGWGNAPSNAPNAMKSSSKSMQDGWGESDGPVTGTRHSSWEEEEEGGVWNTAGSQGSSSSHNSTSWGQGGKKTQMKCSLKGGNSDSWMNPISKQFSNMGLLSQTEDIPGNKMDLSVGGLPDKKFEVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGMHTPVQPLNPSPNIRAQVPPQFLSPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQQLLQSQRKLSQAVRQQQEQQIARMVSALQQQQQRQPGMKHSPSHPVGPKPHIDSMVPNPLNVGLSDLQTKGQIPGYGSGFGSSSMDYGMVGGKEAGTESRFKQWTMMEGLPSVASQDANMHKNGAIVPPGKARGGSPYNQFDIIQGDPLGGHAGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFSDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTGLPRPPPGLTNPKPSSPWNSTAPRSVRGWGAQDSRLASGEKDHLITPLIRPEECAFYSTNGLEERKLQLPASTWSDGSSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYNTKQEAAKAQTALHMCVLGNTTILAEFATDEEVSRFLTQAQPPTPAATPSAPTAGWQSLETGQNQTDPVGPALNLFGGSTGLGQWSSGGGGSSGGDLTGASLWGPPNYSSSLWGVPSVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_015288799.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015288799.2

ORF Insert Sequence:

ATGCAAACTG ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGGTCAG TGAGATGGAA 
CATGAGGATT TTGTAATGGA GGGGCATGGC AAGACTCCAC CTCCTGGTGA AGAAAACAAA 
CAAGAGAAGG AGCAAGAAAG GGAAGAACAG TTAATGGAAG ACAAGAAAAG GAAGAAGGAG 
GATAAGAAAA AGAAGGAATC TGCTCAGAAG GTCACAGATC AAAAAACCAA AGTGCCCGAA 
GTGACGAAAC CAAGTTTAAG CCAACCAGTG GCTGCCAGCC CAATTGGCAA CTCTCCATCG 
CCACCAGTCA ATGGTGGCAA CAATGCCAAA AGGGTGGCAG TGCCGAACGG ACAACCGCCA 
AGCGCCGCCC GCTACATGCC TCGGGAGGTG CCGCCGCGAT TCCGTTGCCA GCAGGACCAC 
AAAGTGTTAC TGAAACGTGG GCAGCCCCCT CCACCATCCT GTATGCTCCT TGGGGGTGGA 
GCAGGGCCTC CTCCCCCTAC AGCACCAGGA GCAAACCCAA ACAACGCACA ACCAGTGACA 
GGAGCACTGC TGCAGAGCGA CAGTGGGACT GCAACAGATT CAACAATTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGT CCTGGAGCCT CCTCCAGCAG CGGCACCAAC 
GCCAACCCAA CTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGTATCG CCAGCAAAGA TGCTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCGAACC CTGGCTTGGA GAAGAACACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGAAGCC AGTGCCAGTC TGGAAGCGCT GGAAATGAAT GTAATCTTGG GGCCTGGAAA 
TCTGATCCCA AGGCTAAATC TGTTCAATCT TCCAACCCTG CTACCGAGAG TAACAGTGGA 
CTAAGTAATT GGAGGAACTT GACTGGACAA GATAGAATTG GTTCTGGTTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCGTCTGCT TGGCCAGCAC TGGTCCAAGA GGGCAATTCT 
AGGAAAGGGA CTTCGGAATC TGATAGTGGT AGCTCCAATG CACAGATTAG CACAGTAGGT 
CAGACCTCTA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAACTT TGTCTCTGGC 
AGAGAACAGG CTCAAATTCA TAACACTGAT GGACCAAAAA ATGGAAACAC TAACTCCTTG 
AACTTAAGTT CACCAAACCC TATGGAGAAT AAGGGAATGC CCTTTGAAAT GGGCTTGGGG 
AACCCTTCCA GGAGCACTGA CACCCCTTCA CAAAGCACTG GAGAAAGAAA GACTGGGAGT 
GTTGGATCTT GGGGTACAGC TAGGGGGTCT TCTGGAACTG ACACAGTCTC TGGACAGAGC 
AATTCTGGAA ACCATGGGAA CAATGGAAAG GATAGGGAGG ACTCCTGGAA AGGAGCTTCT 
GTTCAAAAAC CTAATGGGTC AAGAAGCGAT TCTTGGGATA ACAATAACCG GTCTACGGGT 
GGTGGGTCTT GGAACTTTGG CCCTCAGCAT GCAAATGAAA GCAAATGGGG TGAAGGGAAC 
AAATTAACAT CTGGGGTCTC TCAGGGAGAA TGGAAACAGC TGTCTGGGTC TGATGAACTG 
AAGATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAAAAAGGCC ACCCTCTTCC TGAAAACCAA GGCAATTCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CTGCAGGAAG TGAGGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGACGC CGATCATACA GGCCTACGCA TCCTGATTGC 
CAGGCAGTCT TGCAGACTTT GCTGAGCAGG ACTGATTTGG ACCCCCGAGT GCTTTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAA GATACCGTAT GGGATATTGA AGAGATGCCA 
CGGTCCGAGG GGAAGTCTGA TAAAGGAACT GAGGGGTGGG AGAGCTCTGC CACACAGACA 
AAGAACTCAG GGGGCTGGGG CGATGTACCC AGCCAAAGCA ATCAAATCAA GTCTGGATGG 
GGTGAGCTCT CAACCCCAAC AGAATGGAAG GACCCCAAAA ATACTGGAGG ATGGAATGAC 
TATAAGAACC CCAATTCGTC TAATTGGGGA GGGGGAAGAC AAGAAGAGAA ATCGTCATCT 
TCTTGGAATG ACAGCTCTAG TAAGGATCAG GGGTGGGGTG GACGGCAGCC TAATCAAGGA 
TGGTCTTCTG GAAAGAATGG TTGGGGAGAG GAAGTTGACC AAGCAAAAAA CAGCAACTGG 
GAAAGTGCGG GAAATAAGCC AGCATCTGGT TGGGGTGAAG GTGGGCAAAA TGAGATTGGA 
ACTTGGGGTA ACGGTGCAAA TACAGGCTCT GCTTCAAAGG GTGGTTGGGA TGATTGTAAA 
AGAAATTCAA CGTGGAACGA GACGGGTCGA CAGCCCAACT CCTGGAACAA GCAGCAGCAG 
CAGCAACAGC AGCAACAGCA AGAGACTTCT GGTTCCTGGG GTCCACCGCC ACAAACTCCA 
AGTAATGGGC GACCTCCAAA CCCAAACTGG AACAGCGGAC CACAACCAGC TGCCCCCAAA 
GACGAGGAGC CTAGTGGCTG GGAAGAACCT TCTCCACAGT CAATCAGTCG AAAAATGGAT 
ATTGATGATG GCACTTCAGC GTGGGGAGAT CCTAGCAGTT ATAACTACAA GAATGTGAAC 
CTGTGGGACA AGAACTCACA AGGGGGACAG GCCCCACGGG AACAGAGCCT GCCTACTCCA 
ATGACTAGCA AATCACAAGC ATCAGTCTGG AGCAAAAGCA CTCCACCTGC TCCAGATAAT 
GGTACGTCCG CCTGGGGTGA GCCAAATGAA ACCAGTCCCG GGTGGGGTGA AGTAGATGAT 
ACAGGAGCAT CGACAACAGG CTGGGGGAAT GCACCTTCCA ACGCCCCGAA TGCCATGAAA 
TCTAGTTCTA AATCTATGCA AGATGGCTGG GGAGAGAGTG ATGGGCCAGT GACAGGAACA 
CGTCATTCCA GCTGGGAAGA GGAGGAGGAG GGAGGAGTCT GGAACACAGC AGGCTCTCAG 
GGAAGCAGTT CCTCCCACAA CTCAACAAGC TGGGGGCAAG GAGGAAAGAA AACGCAGATG 
AAGTGCTCGT TAAAAGGAGG AAATAGTGAT TCATGGATGA ACCCTATATC CAAGCAGTTT 
TCAAATATGG GCTTGCTAAG TCAAACTGAG GACATTCCAG GCAATAAGAT GGACTTGTCT 
GTAGGTGGTC TCCCAGATAA GAAGTTTGAG GTAGATAAAC GAGCCATGAA TCTCGGGGAT 
TTTAATGACA TAATGAGAAA GGATCGATCT GGGTTCCGTC CACCTAATTC CAAAGACATG 
GGAACCACAG ATAGTGGGCC TTATTTTGAG AAGGGTGGCA ATCATGGTTT GTTTGGAAAC 
AGCACAGCAC AATCGAGGGG CATGCACACA CCAGTGCAGC CACTAAATCC TTCCCCCAAT 
ATCCGGGCGC AAGTGCCTCC CCAATTTCTT TCTCCCCAGG TTTCGGCCTC CATGCTCAAA 
CAGTTCCCCA ACAGTGGCTT GAATCCAGGT CTTTTCAATG TGGGGCCCCA GCTTTCTCCA 
CAACAGATTG CCATGCTGAG CCAGCTTCCA CAGATTCCCC AGTTTCAATT AGCATGTCAG 
CTCCTCCTTC AGCAGCAGCA GCAGCAGCAG CAGCTGTTAC AGAGCCAGAG GAAGTTATCT 
CAAGCTGTGC GACAGCAGCA GGAGCAACAG ATTGCTCGTA TGGTGAGTGC CCTCCAGCAG 
CAGCAGCAGA GGCAGCCTGG CATGAAGCAT TCACCATCCC ACCCTGTTGG GCCTAAGCCA 
CATATAGACA GCATGGTACC CAATCCTTTG AATGTGGGTC TTTCAGATTT ACAGACCAAA 
GGACAAATAC CTGGATACGG TTCAGGCTTT GGCTCAAGCA GCATGGATTA TGGCATGGTG 
GGAGGGAAAG AAGCTGGAAC AGAATCCCGC TTTAAACAGT GGACTATGAT GGAAGGGCTG 
CCCTCTGTGG CTTCACAAGA TGCCAATATG CACAAAAATG GTGCAATAGT GCCCCCTGGA 
AAGGCTCGCG GAGGATCGCC CTACAACCAG TTCGACATAA TTCAGGGCGA CCCACTAGGT 
GGCCATGCAG GCCCTGCTGG TGATAGTTGG TTACCTGCCA AATCTCCACC GACAAACAAG 
ATCGGAAGCA AATCCAGCAA CGCCAGCTGG CCTCCAGAGT TCCAACCAGG AGTGCCATGG 
AAAGGTATAC AAAACATTGA CCCTGAATCT GACCCCTACG TGACCCCTGG AAGTGTGCTG 
GGGGGGACAG CCACATCTCC CATTGTAGAT ACTGACCACC AACTTCTGCG GGACAACACC 
ACAGGGTCTA ATTCTTCCCT CAACACCTCG CTGCCTTCAC CTGGTGCCTG GCCCTACAGT 
GCCTCTGACA ATTCCTTCAC CAACGTTCAT AGCACTTCAG CAAAATTCAG TGATTACAAA 
TCAACATGGT CCCCAGATCC CATAGGACAC AATCCCACTC ATCTCTCCAA CAAGATGTGG 
AAAAACCATA TTTCCTCAAG GAACACTACA GGGCTGCCTC GCCCGCCTCC TGGCCTGACC 
AACCCCAAAC CATCATCTCC ATGGAACAGC ACAGCACCCC GATCGGTCAG GGGCTGGGGG 
GCACAGGACT CAAGGCTTGC CTCTGGTGAG AAGGATCATC TGATAACGCC ATTGATCAGA 
CCTGAGGAGT GTGCATTTTA CAGCACTAAT GGTTTGGAGG AGAGAAAGTT GCAGTTGCCT 
GCATCTACTT GGAGTGATGG TAGCTCAGTT CGTCCTAGCT ACTGGCTGGT TCTTCACAAT 
CTCACTCCAC AGATCGATGG GTCAACCTTG AGGACGATCT GCATGCAGCA TGGTCCACTG 
CTGACATTCC ATCTGAACCT GACCCAGGGC ACCGCTCTCA TCCGATACAA CACCAAACAG 
GAGGCGGCCA AGGCCCAGAC TGCACTGCAC ATGTGTGTGT TGGGAAACAC TACCATCCTT 
GCTGAGTTTG CCACAGATGA AGAGGTTAGT CGCTTTTTGA CACAAGCTCA GCCCCCTACA 
CCTGCAGCAA CCCCAAGTGC ACCCACGGCA GGCTGGCAAT CCCTGGAGAC AGGACAGAAC 
CAGACAGATC CAGTTGGACC TGCTTTGAAT CTCTTTGGTG GGTCAACAGG GCTTGGGCAG 
TGGAGCAGTG GTGGCGGCGG CAGCAGTGGG GGTGATCTCA CTGGCGCTTC GTTGTGGGGG 
CCCCCAAACT ATTCTTCAAG CTTGTGGGGG GTCCCAAGCG TAGAGGATCC ACACAGAATG 
GGCAGCCCTG CTCCCTTACT ACCTGGAGAC CTTCTGGGAG GAGGGTCGGA TTCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_015288799.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015144285.1 Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X6, mRNA.

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CloneID	OGa29594
Clone ID Related Accession (Same CDS sequence)	XM_015288799.2 , XM_015288799.1
Accession Version	XM_015288799.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	trinucleotide repeat-containing gene 6B protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455714.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACTG ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGGTCAG TGAGATGGAA 
CATGAGGATT TTGTAATGGA GGGGCATGGC AAGACTCCAC CTCCTGGTGA AGAAAACAAA 
CAAGAGAAGG AGCAAGAAAG GGAAGAACAG TTAATGGAAG ACAAGAAAAG GAAGAAGGAG 
GATAAGAAAA AGAAGGAATC TGCTCAGAAG GTCACAGATC AAAAAACCAA AGTGCCCGAA 
GTGACGAAAC CAAGTTTAAG CCAACCAGTG GCTGCCAGCC CAATTGGCAA CTCTCCATCG 
CCACCAGTCA ATGGTGGCAA CAATGCCAAA AGGGTGGCAG TGCCGAACGG ACAACCGCCA 
AGCGCCGCCC GCTACATGCC TCGGGAGGTG CCGCCGCGAT TCCGTTGCCA GCAGGACCAC 
AAAGTGTTAC TGAAACGTGG GCAGCCCCCT CCACCATCCT GTATGCTCCT TGGGGGTGGA 
GCAGGGCCTC CTCCCCCTAC AGCACCAGGA GCAAACCCAA ACAACGCACA ACCAGTGACA 
GGAGCACTGC TGCAGAGCGA CAGTGGGACT GCAACAGATT CAACAATTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGT CCTGGAGCCT CCTCCAGCAG CGGCACCAAC 
GCCAACCCAA CTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGTATCG CCAGCAAAGA TGCTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCGAACC CTGGCTTGGA GAAGAACACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGAAGCC AGTGCCAGTC TGGAAGCGCT GGAAATGAAT GTAATCTTGG GGCCTGGAAA 
TCTGATCCCA AGGCTAAATC TGTTCAATCT TCCAACCCTG CTACCGAGAG TAACAGTGGA 
CTAAGTAATT GGAGGAACTT GACTGGACAA GATAGAATTG GTTCTGGTTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCGTCTGCT TGGCCAGCAC TGGTCCAAGA GGGCAATTCT 
AGGAAAGGGA CTTCGGAATC TGATAGTGGT AGCTCCAATG CACAGATTAG CACAGTAGGT 
CAGACCTCTA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAACTT TGTCTCTGGC 
AGAGAACAGG CTCAAATTCA TAACACTGAT GGACCAAAAA ATGGAAACAC TAACTCCTTG 
AACTTAAGTT CACCAAACCC TATGGAGAAT AAGGGAATGC CCTTTGAAAT GGGCTTGGGG 
AACCCTTCCA GGAGCACTGA CACCCCTTCA CAAAGCACTG GAGAAAGAAA GACTGGGAGT 
GTTGGATCTT GGGGTACAGC TAGGGGGTCT TCTGGAACTG ACACAGTCTC TGGACAGAGC 
AATTCTGGAA ACCATGGGAA CAATGGAAAG GATAGGGAGG ACTCCTGGAA AGGAGCTTCT 
GTTCAAAAAC CTAATGGGTC AAGAAGCGAT TCTTGGGATA ACAATAACCG GTCTACGGGT 
GGTGGGTCTT GGAACTTTGG CCCTCAGCAT GCAAATGAAA GCAAATGGGG TGAAGGGAAC 
AAATTAACAT CTGGGGTCTC TCAGGGAGAA TGGAAACAGC TGTCTGGGTC TGATGAACTG 
AAGATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAAAAAGGCC ACCCTCTTCC TGAAAACCAA GGCAATTCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CTGCAGGAAG TGAGGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGACGC CGATCATACA GGCCTACGCA TCCTGATTGC 
CAGGCAGTCT TGCAGACTTT GCTGAGCAGG ACTGATTTGG ACCCCCGAGT GCTTTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAA GATACCGTAT GGGATATTGA AGAGATGCCA 
CGGTCCGAGG GGAAGTCTGA TAAAGGAACT GAGGGGTGGG AGAGCTCTGC CACACAGACA 
AAGAACTCAG GGGGCTGGGG CGATGTACCC AGCCAAAGCA ATCAAATCAA GTCTGGATGG 
GGTGAGCTCT CAACCCCAAC AGAATGGAAG GACCCCAAAA ATACTGGAGG ATGGAATGAC 
TATAAGAACC CCAATTCGTC TAATTGGGGA GGGGGAAGAC AAGAAGAGAA ATCGTCATCT 
TCTTGGAATG ACAGCTCTAG TAAGGATCAG GGGTGGGGTG GACGGCAGCC TAATCAAGGA 
TGGTCTTCTG GAAAGAATGG TTGGGGAGAG GAAGTTGACC AAGCAAAAAA CAGCAACTGG 
GAAAGTGCGG GAAATAAGCC AGCATCTGGT TGGGGTGAAG GTGGGCAAAA TGAGATTGGA 
ACTTGGGGTA ACGGTGCAAA TACAGGCTCT GCTTCAAAGG GTGGTTGGGA TGATTGTAAA 
AGAAATTCAA CGTGGAACGA GACGGGTCGA CAGCCCAACT CCTGGAACAA GCAGCAGCAG 
CAGCAACAGC AGCAACAGCA AGAGACTTCT GGTTCCTGGG GTCCACCGCC ACAAACTCCA 
AGTAATGGGC GACCTCCAAA CCCAAACTGG AACAGCGGAC CACAACCAGC TGCCCCCAAA 
GACGAGGAGC CTAGTGGCTG GGAAGAACCT TCTCCACAGT CAATCAGTCG AAAAATGGAT 
ATTGATGATG GCACTTCAGC GTGGGGAGAT CCTAGCAGTT ATAACTACAA GAATGTGAAC 
CTGTGGGACA AGAACTCACA AGGGGGACAG GCCCCACGGG AACAGAGCCT GCCTACTCCA 
ATGACTAGCA AATCACAAGC ATCAGTCTGG AGCAAAAGCA CTCCACCTGC TCCAGATAAT 
GGTACGTCCG CCTGGGGTGA GCCAAATGAA ACCAGTCCCG GGTGGGGTGA AGTAGATGAT 
ACAGGAGCAT CGACAACAGG CTGGGGGAAT GCACCTTCCA ACGCCCCGAA TGCCATGAAA 
TCTAGTTCTA AATCTATGCA AGATGGCTGG GGAGAGAGTG ATGGGCCAGT GACAGGAACA 
CGTCATTCCA GCTGGGAAGA GGAGGAGGAG GGAGGAGTCT GGAACACAGC AGGCTCTCAG 
GGAAGCAGTT CCTCCCACAA CTCAACAAGC TGGGGGCAAG GAGGAAAGAA AACGCAGATG 
AAGTGCTCGT TAAAAGGAGG AAATAGTGAT TCATGGATGA ACCCTATATC CAAGCAGTTT 
TCAAATATGG GCTTGCTAAG TCAAACTGAG GACATTCCAG GCAATAAGAT GGACTTGTCT 
GTAGGTGGTC TCCCAGATAA GAAGTTTGAG GTAGATAAAC GAGCCATGAA TCTCGGGGAT 
TTTAATGACA TAATGAGAAA GGATCGATCT GGGTTCCGTC CACCTAATTC CAAAGACATG 
GGAACCACAG ATAGTGGGCC TTATTTTGAG AAGGGTGGCA ATCATGGTTT GTTTGGAAAC 
AGCACAGCAC AATCGAGGGG CATGCACACA CCAGTGCAGC CACTAAATCC TTCCCCCAAT 
ATCCGGGCGC AAGTGCCTCC CCAATTTCTT TCTCCCCAGG TTTCGGCCTC CATGCTCAAA 
CAGTTCCCCA ACAGTGGCTT GAATCCAGGT CTTTTCAATG TGGGGCCCCA GCTTTCTCCA 
CAACAGATTG CCATGCTGAG CCAGCTTCCA CAGATTCCCC AGTTTCAATT AGCATGTCAG 
CTCCTCCTTC AGCAGCAGCA GCAGCAGCAG CAGCTGTTAC AGAGCCAGAG GAAGTTATCT 
CAAGCTGTGC GACAGCAGCA GGAGCAACAG ATTGCTCGTA TGGTGAGTGC CCTCCAGCAG 
CAGCAGCAGA GGCAGCCTGG CATGAAGCAT TCACCATCCC ACCCTGTTGG GCCTAAGCCA 
CATATAGACA GCATGGTACC CAATCCTTTG AATGTGGGTC TTTCAGATTT ACAGACCAAA 
GGACAAATAC CTGGATACGG TTCAGGCTTT GGCTCAAGCA GCATGGATTA TGGCATGGTG 
GGAGGGAAAG AAGCTGGAAC AGAATCCCGC TTTAAACAGT GGACTATGAT GGAAGGGCTG 
CCCTCTGTGG CTTCACAAGA TGCCAATATG CACAAAAATG GTGCAATAGT GCCCCCTGGA 
AAGGCTCGCG GAGGATCGCC CTACAACCAG TTCGACATAA TTCAGGGCGA CCCACTAGGT 
GGCCATGCAG GCCCTGCTGG TGATAGTTGG TTACCTGCCA AATCTCCACC GACAAACAAG 
ATCGGAAGCA AATCCAGCAA CGCCAGCTGG CCTCCAGAGT TCCAACCAGG AGTGCCATGG 
AAAGGTATAC AAAACATTGA CCCTGAATCT GACCCCTACG TGACCCCTGG AAGTGTGCTG 
GGGGGGACAG CCACATCTCC CATTGTAGAT ACTGACCACC AACTTCTGCG GGACAACACC 
ACAGGGTCTA ATTCTTCCCT CAACACCTCG CTGCCTTCAC CTGGTGCCTG GCCCTACAGT 
GCCTCTGACA ATTCCTTCAC CAACGTTCAT AGCACTTCAG CAAAATTCAG TGATTACAAA 
TCAACATGGT CCCCAGATCC CATAGGACAC AATCCCACTC ATCTCTCCAA CAAGATGTGG 
AAAAACCATA TTTCCTCAAG GAACACTACA GGGCTGCCTC GCCCGCCTCC TGGCCTGACC 
AACCCCAAAC CATCATCTCC ATGGAACAGC ACAGCACCCC GATCGGTCAG GGGCTGGGGG 
GCACAGGACT CAAGGCTTGC CTCTGGTGAG AAGGATCATC TGATAACGCC ATTGATCAGA 
CCTGAGGAGT GTGCATTTTA CAGCACTAAT GGTTTGGAGG AGAGAAAGTT GCAGTTGCCT 
GCATCTACTT GGAGTGATGG TAGCTCAGTT CGTCCTAGCT ACTGGCTGGT TCTTCACAAT 
CTCACTCCAC AGATCGATGG GTCAACCTTG AGGACGATCT GCATGCAGCA TGGTCCACTG 
CTGACATTCC ATCTGAACCT GACCCAGGGC ACCGCTCTCA TCCGATACAA CACCAAACAG 
GAGGCGGCCA AGGCCCAGAC TGCACTGCAC ATGTGTGTGT TGGGAAACAC TACCATCCTT 
GCTGAGTTTG CCACAGATGA AGAGGTTAGT CGCTTTTTGA CACAAGCTCA GCCCCCTACA 
CCTGCAGCAA CCCCAAGTGC ACCCACGGCA GGCTGGCAAT CCCTGGAGAC AGGACAGAAC 
CAGACAGATC CAGTTGGACC TGCTTTGAAT CTCTTTGGTG GGTCAACAGG GCTTGGGCAG 
TGGAGCAGTG GTGGCGGCGG CAGCAGTGGG GGTGATCTCA CTGGCGCTTC GTTGTGGGGG 
CCCCCAAACT ATTCTTCAAG CTTGTGGGGG GTCCCAAGCG TAGAGGATCC ACACAGAATG 
GGCAGCCCTG CTCCCTTACT ACCTGGAGAC CTTCTGGGAG GAGGGTCGGA TTCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015144285.1
CDS	538..6057
Translation	MQTDEGEILEEGSPKVSEMEHEDFVMEGHGKTPPPGEENKQEKEQEREEQLMEDKKRKKEDKKKKESAQKVTDQKTKVPEVTKPSLSQPVAASPIGNSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPPTAPGANPNNAQPVTGALLQSDSGTATDSTIGGAAASNYANSTWGPGASSSSGTNANPTHIWDKVIVDGSDMEEWPCIASKDAESSSENTTDNNSASNPGLEKNTLPGSTTSNKGKGSQCQSGSAGNECNLGAWKSDPKAKSVQSSNPATESNSGLSNWRNLTGQDRIGSGSGFSNFNPNSNPSAWPALVQEGNSRKGTSESDSGSSNAQISTVGQTSREQQSKMENAGVNFVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFEMGLGNPSRSTDTPSQSTGERKTGSVGSWGTARGSSGTDTVSGQSNSGNHGNNGKDREDSWKGASVQKPNGSRSDSWDNNNRSTGGGSWNFGPQHANESKWGEGNKLTSGVSQGEWKQLSGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNSQAPCWGRSSSSAGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEMPRSEGKSDKGTEGWESSATQTKNSGGWGDVPSQSNQIKSGWGELSTPTEWKDPKNTGGWNDYKNPNSSNWGGGRQEEKSSSSWNDSSSKDQGWGGRQPNQGWSSGKNGWGEEVDQAKNSNWESAGNKPASGWGEGGQNEIGTWGNGANTGSASKGGWDDCKRNSTWNETGRQPNSWNKQQQQQQQQQQETSGSWGPPPQTPSNGRPPNPNWNSGPQPAAPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPSSYNYKNVNLWDKNSQGGQAPREQSLPTPMTSKSQASVWSKSTPPAPDNGTSAWGEPNETSPGWGEVDDTGASTTGWGNAPSNAPNAMKSSSKSMQDGWGESDGPVTGTRHSSWEEEEEGGVWNTAGSQGSSSSHNSTSWGQGGKKTQMKCSLKGGNSDSWMNPISKQFSNMGLLSQTEDIPGNKMDLSVGGLPDKKFEVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGMHTPVQPLNPSPNIRAQVPPQFLSPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQQLLQSQRKLSQAVRQQQEQQIARMVSALQQQQQRQPGMKHSPSHPVGPKPHIDSMVPNPLNVGLSDLQTKGQIPGYGSGFGSSSMDYGMVGGKEAGTESRFKQWTMMEGLPSVASQDANMHKNGAIVPPGKARGGSPYNQFDIIQGDPLGGHAGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFSDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTGLPRPPPGLTNPKPSSPWNSTAPRSVRGWGAQDSRLASGEKDHLITPLIRPEECAFYSTNGLEERKLQLPASTWSDGSSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYNTKQEAAKAQTALHMCVLGNTTILAEFATDEEVSRFLTQAQPPTPAATPSAPTAGWQSLETGQNQTDPVGPALNLFGGSTGLGQWSSGGGGSSGGDLTGASLWGPPNYSSSLWGVPSVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_015288799.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015288799.1

ORF Insert Sequence:

ATGCAAACTG ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGGTCAG TGAGATGGAA 
CATGAGGATT TTGTAATGGA GGGGCATGGC AAGACTCCAC CTCCTGGTGA AGAAAACAAA 
CAAGAGAAGG AGCAAGAAAG GGAAGAACAG TTAATGGAAG ACAAGAAAAG GAAGAAGGAG 
GATAAGAAAA AGAAGGAATC TGCTCAGAAG GTCACAGATC AAAAAACCAA AGTGCCCGAA 
GTGACGAAAC CAAGTTTAAG CCAACCAGTG GCTGCCAGCC CAATTGGCAA CTCTCCATCG 
CCACCAGTCA ATGGTGGCAA CAATGCCAAA AGGGTGGCAG TGCCGAACGG ACAACCGCCA 
AGCGCCGCCC GCTACATGCC TCGGGAGGTG CCGCCGCGAT TCCGTTGCCA GCAGGACCAC 
AAAGTGTTAC TGAAACGTGG GCAGCCCCCT CCACCATCCT GTATGCTCCT TGGGGGTGGA 
GCAGGGCCTC CTCCCCCTAC AGCACCAGGA GCAAACCCAA ACAACGCACA ACCAGTGACA 
GGAGCACTGC TGCAGAGCGA CAGTGGGACT GCAACAGATT CAACAATTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGT CCTGGAGCCT CCTCCAGCAG CGGCACCAAC 
GCCAACCCAA CTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGTATCG CCAGCAAAGA TGCTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCGAACC CTGGCTTGGA GAAGAACACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGAAGCC AGTGCCAGTC TGGAAGCGCT GGAAATGAAT GTAATCTTGG GGCCTGGAAA 
TCTGATCCCA AGGCTAAATC TGTTCAATCT TCCAACCCTG CTACCGAGAG TAACAGTGGA 
CTAAGTAATT GGAGGAACTT GACTGGACAA GATAGAATTG GTTCTGGTTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCGTCTGCT TGGCCAGCAC TGGTCCAAGA GGGCAATTCT 
AGGAAAGGGA CTTCGGAATC TGATAGTGGT AGCTCCAATG CACAGATTAG CACAGTAGGT 
CAGACCTCTA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAACTT TGTCTCTGGC 
AGAGAACAGG CTCAAATTCA TAACACTGAT GGACCAAAAA ATGGAAACAC TAACTCCTTG 
AACTTAAGTT CACCAAACCC TATGGAGAAT AAGGGAATGC CCTTTGAAAT GGGCTTGGGG 
AACCCTTCCA GGAGCACTGA CACCCCTTCA CAAAGCACTG GAGAAAGAAA GACTGGGAGT 
GTTGGATCTT GGGGTACAGC TAGGGGGTCT TCTGGAACTG ACACAGTCTC TGGACAGAGC 
AATTCTGGAA ACCATGGGAA CAATGGAAAG GATAGGGAGG ACTCCTGGAA AGGAGCTTCT 
GTTCAAAAAC CTAATGGGTC AAGAAGCGAT TCTTGGGATA ACAATAACCG GTCTACGGGT 
GGTGGGTCTT GGAACTTTGG CCCTCAGCAT GCAAATGAAA GCAAATGGGG TGAAGGGAAC 
AAATTAACAT CTGGGGTCTC TCAGGGAGAA TGGAAACAGC TGTCTGGGTC TGATGAACTG 
AAGATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAAAAAGGCC ACCCTCTTCC TGAAAACCAA GGCAATTCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CTGCAGGAAG TGAGGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGACGC CGATCATACA GGCCTACGCA TCCTGATTGC 
CAGGCAGTCT TGCAGACTTT GCTGAGCAGG ACTGATTTGG ACCCCCGAGT GCTTTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAA GATACCGTAT GGGATATTGA AGAGATGCCA 
CGGTCCGAGG GGAAGTCTGA TAAAGGAACT GAGGGGTGGG AGAGCTCTGC CACACAGACA 
AAGAACTCAG GGGGCTGGGG CGATGTACCC AGCCAAAGCA ATCAAATCAA GTCTGGATGG 
GGTGAGCTCT CAACCCCAAC AGAATGGAAG GACCCCAAAA ATACTGGAGG ATGGAATGAC 
TATAAGAACC CCAATTCGTC TAATTGGGGA GGGGGAAGAC AAGAAGAGAA ATCGTCATCT 
TCTTGGAATG ACAGCTCTAG TAAGGATCAG GGGTGGGGTG GACGGCAGCC TAATCAAGGA 
TGGTCTTCTG GAAAGAATGG TTGGGGAGAG GAAGTTGACC AAGCAAAAAA CAGCAACTGG 
GAAAGTGCGG GAAATAAGCC AGCATCTGGT TGGGGTGAAG GTGGGCAAAA TGAGATTGGA 
ACTTGGGGTA ACGGTGCAAA TACAGGCTCT GCTTCAAAGG GTGGTTGGGA TGATTGTAAA 
AGAAATTCAA CGTGGAACGA GACGGGTCGA CAGCCCAACT CCTGGAACAA GCAGCAGCAG 
CAGCAACAGC AGCAACAGCA AGAGACTTCT GGTTCCTGGG GTCCACCGCC ACAAACTCCA 
AGTAATGGGC GACCTCCAAA CCCAAACTGG AACAGCGGAC CACAACCAGC TGCCCCCAAA 
GACGAGGAGC CTAGTGGCTG GGAAGAACCT TCTCCACAGT CAATCAGTCG AAAAATGGAT 
ATTGATGATG GCACTTCAGC GTGGGGAGAT CCTAGCAGTT ATAACTACAA GAATGTGAAC 
CTGTGGGACA AGAACTCACA AGGGGGACAG GCCCCACGGG AACAGAGCCT GCCTACTCCA 
ATGACTAGCA AATCACAAGC ATCAGTCTGG AGCAAAAGCA CTCCACCTGC TCCAGATAAT 
GGTACGTCCG CCTGGGGTGA GCCAAATGAA ACCAGTCCCG GGTGGGGTGA AGTAGATGAT 
ACAGGAGCAT CGACAACAGG CTGGGGGAAT GCACCTTCCA ACGCCCCGAA TGCCATGAAA 
TCTAGTTCTA AATCTATGCA AGATGGCTGG GGAGAGAGTG ATGGGCCAGT GACAGGAACA 
CGTCATTCCA GCTGGGAAGA GGAGGAGGAG GGAGGAGTCT GGAACACAGC AGGCTCTCAG 
GGAAGCAGTT CCTCCCACAA CTCAACAAGC TGGGGGCAAG GAGGAAAGAA AACGCAGATG 
AAGTGCTCGT TAAAAGGAGG AAATAGTGAT TCATGGATGA ACCCTATATC CAAGCAGTTT 
TCAAATATGG GCTTGCTAAG TCAAACTGAG GACATTCCAG GCAATAAGAT GGACTTGTCT 
GTAGGTGGTC TCCCAGATAA GAAGTTTGAG GTAGATAAAC GAGCCATGAA TCTCGGGGAT 
TTTAATGACA TAATGAGAAA GGATCGATCT GGGTTCCGTC CACCTAATTC CAAAGACATG 
GGAACCACAG ATAGTGGGCC TTATTTTGAG AAGGGTGGCA ATCATGGTTT GTTTGGAAAC 
AGCACAGCAC AATCGAGGGG CATGCACACA CCAGTGCAGC CACTAAATCC TTCCCCCAAT 
ATCCGGGCGC AAGTGCCTCC CCAATTTCTT TCTCCCCAGG TTTCGGCCTC CATGCTCAAA 
CAGTTCCCCA ACAGTGGCTT GAATCCAGGT CTTTTCAATG TGGGGCCCCA GCTTTCTCCA 
CAACAGATTG CCATGCTGAG CCAGCTTCCA CAGATTCCCC AGTTTCAATT AGCATGTCAG 
CTCCTCCTTC AGCAGCAGCA GCAGCAGCAG CAGCTGTTAC AGAGCCAGAG GAAGTTATCT 
CAAGCTGTGC GACAGCAGCA GGAGCAACAG ATTGCTCGTA TGGTGAGTGC CCTCCAGCAG 
CAGCAGCAGA GGCAGCCTGG CATGAAGCAT TCACCATCCC ACCCTGTTGG GCCTAAGCCA 
CATATAGACA GCATGGTACC CAATCCTTTG AATGTGGGTC TTTCAGATTT ACAGACCAAA 
GGACAAATAC CTGGATACGG TTCAGGCTTT GGCTCAAGCA GCATGGATTA TGGCATGGTG 
GGAGGGAAAG AAGCTGGAAC AGAATCCCGC TTTAAACAGT GGACTATGAT GGAAGGGCTG 
CCCTCTGTGG CTTCACAAGA TGCCAATATG CACAAAAATG GTGCAATAGT GCCCCCTGGA 
AAGGCTCGCG GAGGATCGCC CTACAACCAG TTCGACATAA TTCAGGGCGA CCCACTAGGT 
GGCCATGCAG GCCCTGCTGG TGATAGTTGG TTACCTGCCA AATCTCCACC GACAAACAAG 
ATCGGAAGCA AATCCAGCAA CGCCAGCTGG CCTCCAGAGT TCCAACCAGG AGTGCCATGG 
AAAGGTATAC AAAACATTGA CCCTGAATCT GACCCCTACG TGACCCCTGG AAGTGTGCTG 
GGGGGGACAG CCACATCTCC CATTGTAGAT ACTGACCACC AACTTCTGCG GGACAACACC 
ACAGGGTCTA ATTCTTCCCT CAACACCTCG CTGCCTTCAC CTGGTGCCTG GCCCTACAGT 
GCCTCTGACA ATTCCTTCAC CAACGTTCAT AGCACTTCAG CAAAATTCAG TGATTACAAA 
TCAACATGGT CCCCAGATCC CATAGGACAC AATCCCACTC ATCTCTCCAA CAAGATGTGG 
AAAAACCATA TTTCCTCAAG GAACACTACA GGGCTGCCTC GCCCGCCTCC TGGCCTGACC 
AACCCCAAAC CATCATCTCC ATGGAACAGC ACAGCACCCC GATCGGTCAG GGGCTGGGGG 
GCACAGGACT CAAGGCTTGC CTCTGGTGAG AAGGATCATC TGATAACGCC ATTGATCAGA 
CCTGAGGAGT GTGCATTTTA CAGCACTAAT GGTTTGGAGG AGAGAAAGTT GCAGTTGCCT 
GCATCTACTT GGAGTGATGG TAGCTCAGTT CGTCCTAGCT ACTGGCTGGT TCTTCACAAT 
CTCACTCCAC AGATCGATGG GTCAACCTTG AGGACGATCT GCATGCAGCA TGGTCCACTG 
CTGACATTCC ATCTGAACCT GACCCAGGGC ACCGCTCTCA TCCGATACAA CACCAAACAG 
GAGGCGGCCA AGGCCCAGAC TGCACTGCAC ATGTGTGTGT TGGGAAACAC TACCATCCTT 
GCTGAGTTTG CCACAGATGA AGAGGTTAGT CGCTTTTTGA CACAAGCTCA GCCCCCTACA 
CCTGCAGCAA CCCCAAGTGC ACCCACGGCA GGCTGGCAAT CCCTGGAGAC AGGACAGAAC 
CAGACAGATC CAGTTGGACC TGCTTTGAAT CTCTTTGGTG GGTCAACAGG GCTTGGGCAG 
TGGAGCAGTG GTGGCGGCGG CAGCAGTGGG GGTGATCTCA CTGGCGCTTC GTTGTGGGGG 
CCCCCAAACT ATTCTTCAAG CTTGTGGGGG GTCCCAAGCG TAGAGGATCC ACACAGAATG 
GGCAGCCCTG CTCCCTTACT ACCTGGAGAC CTTCTGGGAG GAGGGTCGGA TTCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_015288805.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015144291.1 Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X7, mRNA.

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CloneID	OGa29595
Clone ID Related Accession (Same CDS sequence)	XM_015288805.2 , XM_015288805.1
Accession Version	XM_015288805.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	trinucleotide repeat-containing gene 6B protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288805.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACTG ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGGTCAG TGAGATGGAA 
CATGAGGATT TTGTAATGGA GGGGCATGGC AAGACTCCAC CTCCTGGTGA AGAAAACAAA 
CAAGAGAAGG AGCAAGAAAG GGAAGAACAG TTAATGGAAG ACAAGAAAAG GAAGAAGGAG 
GATAAGAAAA AGAAGGAATC TGCTCAGAAG GTCACAGATC AAAAAACCAA AGTGCCCGAA 
GTGACGAAAC CAAGTTTAAG CCAACCAGTG GCTGCCAGCC CAATTGGCAA CTCTCCATCG 
CCACCAGTCA ATGGTGGCAA CAATGCCAAA AGGGTGGCAG TGCCGAACGG ACAACCGCCA 
AGCGCCGCCC GCTACATGCC TCGGGAGGTG CCGCCGCGAT TCCGTTGCCA GCAGGACCAC 
AAAGTGTTAC TGAAACGTGG GCAGCCCCCT CCACCATCCT GTATGCTCCT TGGGGGTGGA 
GCAGGGCCTC CTCCCCCTAC AGCACCAGGA GCAAACCCAA ACAACGCACA ACCAGTGACA 
GGAGCACTGC TGCAGAGCGA CAGTGGGACT GCAACAGATT CAACAATTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGT CCTGGAGCCT CCTCCAGCAG CGGCACCAAC 
GCCAACCCAA CTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGTATCG CCAGCAAAGA TGCTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCGAACC CTGGCTTGGA GAAGAACACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGAAGCC AGTGCCAGTC TGGAAGCGCT GGAAATGAAT GTAATCTTGG GGCCTGGAAA 
TCTGATCCCA AGGCTAAATC TGTTCAATCT TCCAACCCTG CTACCGAGAG TAACAGTGGA 
CTAAGTAATT GGAGGAACTT GACTGGACAA GATAGAATTG GTTCTGGTTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCGTCTGCT TGGCCAGCAC TGGTCCAAGA GGGCAATTCT 
AGGAAAGGGA CTTCGGAATC TGATAGTGGT AGCTCCAATG CACAGATTAG CACAGTAGGT 
CAGACCTCTA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAACTT TGTCTCTGGC 
AGAGAACAGG CTCAAATTCA TAACACTGAT GGACCAAAAA ATGGAAACAC TAACTCCTTG 
AACTTAAGTT CACCAAACCC TATGGAGAAT AAGGGAATGC CCTTTGAAAT GGGCTTGGGG 
AACCCTTCCA GGAGCACTGA CACCCCTTCA CAAAGCACTG GAGAAAGAAA GACTGGGAGT 
GTTGGATCTT GGGGTACAGC TAGGGGGTCT TCTGGAACTG ACACAGTCTC TGGACAGAGC 
AATTCTGGAA ACCATGGGAA CAATGGAAAG GATAGGGAGG ACTCCTGGAA AGGAGCTTCT 
GTTCAAAAAC CTAATGGGTC AAGAAGCGAT TCTTGGGATA ACAATAACCG GTCTACGGGT 
GGTGGGTCTT GGAACTTTGG CCCTCAGCAT GCAAATGAAA GCAAATGGGG TGAAGGGAAC 
AAATTAACAT CTGGGGTCTC TCAGGGAGAA TGGAAACAGC TGTCTGGGTC TGATGAACTG 
AAGATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAAAAAGGCC ACCCTCTTCC TGAAAACCAA GGCAATTCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CTGCAGGAAG TGAGGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGACGC CGATCATACA GGCCTACGCA TCCTGATTGC 
CAGGCAGTCT TGCAGACTTT GCTGAGCAGG ACTGATTTGG ACCCCCGAGT GCTTTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAA GATACCGTAT GGGATATTGA AGAGATGCCA 
CGGTCCGAGG GGAAGTCTGA TAAAGGAACT GAGGGGTGGG AGAGCTCTGC CACACAGACA 
AAGAACTCAG GGGGCTGGGG CGATGTACCC AGCCAAAGCA ATCAAATCAA GTCTGGATGG 
GGTGAGCTCT CAACCCCAAC AGAATGGAAG GACCCCAAAA ATACTGGAGG ATGGAATGAC 
TATAAGAACC CCAATTCGTC TAATTGGGGA GGGGGAAGAC AAGAAGAGAA ATCGTCATCT 
TCTTGGAATG ACAGCTCTAG TAAGGATCAG GGGTGGGGTG GACGGCAGCC TAATCAAGGA 
TGGTCTTCTG GAAAGAATGG TTGGGGAGAG GAAGTTGACC AAGCAAAAAA CAGCAACTGG 
GAAAGTGCGG GAAATAAGCC AGCATCTGGT TGGGGTGAAG GTGGGCAAAA TGAGATTGGA 
ACTTGGGGTA ACGGTGCAAA TACAGGCTCT GCTTCAAAGG GTGGTTGGGA TGATTGTAAA 
AGAAATTCAA CGTGGAACGA GACGGGTCGA CAGCCCAACT CCTGGAACAA GCAGCAGCAG 
CAGCAACAGC AGCAACAGCA AGAGACTTCT GGTTCCTGGG GTCCACCGCC ACAAACTCCA 
AGTAATGGGC GACCTCCAAA CCCAAACTGG AACAGCGGAC CACAACCAGC TGCCCCCAAA 
GACGAGGAGC CTAGTGGCTG GGAAGAACCT TCTCCACAGT CAATCAGTCG AAAAATGGAT 
ATTGATGATG GCACTTCAGC GTGGGGAGAT CCTAGCAGTT ATAACTACAA GAATGTGAAC 
CTGTGGGACA AGAACTCACA AGGGGGACAG GCCCCACGGG AACAGAGCCT GCCTACTCCA 
ATGACTAGCA AATCACAAGC ATCAGTCTGG AGCAAAAGCA CTCCACCTGC TCCAGATAAT 
GGTACGTCCG CCTGGGGTGA GCCAAATGAA ACCAGTCCCG GGTGGGGTGA AGTAGATGAT 
ACAGGAGCAT CGACAACAGG CTGGGGGAAT GCACCTTCCA ACGCCCCGAA TGCCATGAAA 
TCTAGTTCTA AATCTATGCA AGATGGCTGG GGAGAGAGTG ATGGGCCAGT GACAGGAACA 
CGTCATTCCA GCTGGGAAGA GGAGGAGGAG GGAGGAGTCT GGAACACAGC AGGCTCTCAG 
GGAAGCAGTT CCTCCCACAA CTCAACAAGC TGGGGGCAAG GAGGAAAGAA AACGCAGATG 
AAGTGCTCGT TAAAAGGAGG AAATAGTGAT TCATGGATGA ACCCTATATC CAAGCAGTTT 
TCAAATATGG GCTTGCTAAG TCAAACTGAG GACATTCCAG GCAATAAGAT GGACTTGTCT 
GTAGGTGGTC TCCCAGATAA GAAGTTTGAG GTAGATAAAC GAGCCATGAA TCTCGGGGAT 
TTTAATGACA TAATGAGAAA GGATCGATCT GGGTTCCGTC CACCTAATTC CAAAGACATG 
GGAACCACAG ATAGTGGGCC TTATTTTGAG AAGGGTGGCA ATCATGGTTT GTTTGGAAAC 
AGCACAGCAC AATCGAGGGG CATGCACACA CCAGTGCAGC CACTAAATCC TTCCCCCAAT 
ATCCGGGCGC AAGTGCCTCC CCAATTTCTT TCTCCCCAGG TTTCGGCCTC CATGCTCAAA 
CAGTTCCCCA ACAGTGGCTT GAATCCAGGT CTTTTCAATG TGGGGCCCCA GCTTTCTCCA 
CAACAGATTG CCATGCTGAG CCAGCTTCCA CAGATTCCCC AGTTTCAATT AGCATGTCAG 
CTCCTCCTTC AGCAGCAGCA GCAGCAGCAG CAGCTGTTAC AGAGCCAGAG GAAGTTATCT 
CAAGCTGTGC GACAGCAGCA GGAGCAACAG ATTGCTCGTA TGGTGAGTGC CCTCCAGCAG 
CAGCAGCAGA GGCAGCCTGG CATGAAGCAT TCACCATCCC ACCCTGTTGG GCCTAAGCCA 
CATATAGACA GCATGGTACC CAATCCTTTG AATGTGGGTC TTTCAGATTT ACAGACCAAA 
GGACAAATAC CTGGATACGG TTCAGGCTTT GGCTCAAGCA GCATGGATTA TGGCATGGTG 
GGAGGGAAAG AAGCTGGAAC AGAATCCCGC TTTAAACAGT GGACTATGAT GGAAGGGCTG 
CCCTCTGTGG CTTCACAAGA TGCCAATATG CACAAAAATG GTGCAATAGT GCCCCCTGGA 
AAGGCTCGCG GAGGATCGCC CTACAACCAG TTCGACATAA TTCAGGGCGA CCCACTAGGT 
GGCCATGCAG GCCCTGCTGG TGATAGTTGG TTACCTGCCA AATCTCCACC GACAAACAAG 
ATCGGAAGCA AATCCAGCAA CGCCAGCTGG CCTCCAGAGT TCCAACCAGG AGTGCCATGG 
AAAGGTATAC AAAACATTGA CCCTGAATCT GACCCCTACG TGACCCCTGG AAGTGTGCTG 
GGGGGGACAG CCACATCTCC CATTGTAGAT ACTGACCACC AACTTCTGCG GGACAACACC 
ACAGGGTCTA ATTCTTCCCT CAACACCTCG CTGCCTTCAC CTGGTGCCTG GCCCTACAGT 
GCCTCTGACA ATTCCTTCAC CAACGTTCAT AGCACTTCAG CAAAATTCAG TGATTACAAA 
TCAACATGGT CCCCAGATCC CATAGGACAC AATCCCACTC ATCTCTCCAA CAAGATGTGG 
AAAAACCATA TTTCCTCAAG GAACACTACA GGGCTGCCTC GCCCGCCTCC TGGCCTGACC 
AACCCCAAAC CATCATCTCC ATGGAACAGC ACAGCACCCC GATCGGTCAG GGGCTGGGGG 
GCACAGGACT CAAGGCTTGC CTCTGCATCT ACTTGGAGTG ATGGTAGCTC AGTTCGTCCT 
AGCTACTGGC TGGTTCTTCA CAATCTCACT CCACAGATCG ATGGGTCAAC CTTGAGGACG 
ATCTGCATGC AGCATGGTCC ACTGCTGACA TTCCATCTGA ACCTGACCCA GGGCACCGCT 
CTCATCCGAT ACAACACCAA ACAGGAGGCG GCCAAGGCCC AGACTGCACT GCACATGTGT 
GTGTTGGGAA ACACTACCAT CCTTGCTGAG TTTGCCACAG ATGAAGAGGT TAGTCGCTTT 
TTGACACAAG CTCAGCCCCC TACACCTGCA GCAACCCCAA GTGCACCCAC GGCAGGCTGG 
CAATCCCTGG AGACAGGACA GAACCAGACA GATCCAGTTG GACCTGCTTT GAATCTCTTT 
GGTGGGTCAA CAGGGCTTGG GCAGTGGAGC AGTGGTGGCG GCGGCAGCAG TGGGGGTGAT 
CTCACTGGCG CTTCGTTGTG GGGGCCCCCA AACTATTCTT CAAGCTTGTG GGGGGTCCCA 
AGCGTAGAGG ATCCACACAG AATGGGCAGC CCTGCTCCCT TACTACCTGG AGACCTTCTG 
GGAGGAGGGT CGGATTCAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015144291.1
CDS	543..5966
Translation	MQTDEGEILEEGSPKVSEMEHEDFVMEGHGKTPPPGEENKQEKEQEREEQLMEDKKRKKEDKKKKESAQKVTDQKTKVPEVTKPSLSQPVAASPIGNSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPPTAPGANPNNAQPVTGALLQSDSGTATDSTIGGAAASNYANSTWGPGASSSSGTNANPTHIWDKVIVDGSDMEEWPCIASKDAESSSENTTDNNSASNPGLEKNTLPGSTTSNKGKGSQCQSGSAGNECNLGAWKSDPKAKSVQSSNPATESNSGLSNWRNLTGQDRIGSGSGFSNFNPNSNPSAWPALVQEGNSRKGTSESDSGSSNAQISTVGQTSREQQSKMENAGVNFVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFEMGLGNPSRSTDTPSQSTGERKTGSVGSWGTARGSSGTDTVSGQSNSGNHGNNGKDREDSWKGASVQKPNGSRSDSWDNNNRSTGGGSWNFGPQHANESKWGEGNKLTSGVSQGEWKQLSGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNSQAPCWGRSSSSAGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEMPRSEGKSDKGTEGWESSATQTKNSGGWGDVPSQSNQIKSGWGELSTPTEWKDPKNTGGWNDYKNPNSSNWGGGRQEEKSSSSWNDSSSKDQGWGGRQPNQGWSSGKNGWGEEVDQAKNSNWESAGNKPASGWGEGGQNEIGTWGNGANTGSASKGGWDDCKRNSTWNETGRQPNSWNKQQQQQQQQQQETSGSWGPPPQTPSNGRPPNPNWNSGPQPAAPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPSSYNYKNVNLWDKNSQGGQAPREQSLPTPMTSKSQASVWSKSTPPAPDNGTSAWGEPNETSPGWGEVDDTGASTTGWGNAPSNAPNAMKSSSKSMQDGWGESDGPVTGTRHSSWEEEEEGGVWNTAGSQGSSSSHNSTSWGQGGKKTQMKCSLKGGNSDSWMNPISKQFSNMGLLSQTEDIPGNKMDLSVGGLPDKKFEVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGMHTPVQPLNPSPNIRAQVPPQFLSPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQQLLQSQRKLSQAVRQQQEQQIARMVSALQQQQQRQPGMKHSPSHPVGPKPHIDSMVPNPLNVGLSDLQTKGQIPGYGSGFGSSSMDYGMVGGKEAGTESRFKQWTMMEGLPSVASQDANMHKNGAIVPPGKARGGSPYNQFDIIQGDPLGGHAGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFSDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTGLPRPPPGLTNPKPSSPWNSTAPRSVRGWGAQDSRLASASTWSDGSSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYNTKQEAAKAQTALHMCVLGNTTILAEFATDEEVSRFLTQAQPPTPAATPSAPTAGWQSLETGQNQTDPVGPALNLFGGSTGLGQWSSGGGGSSGGDLTGASLWGPPNYSSSLWGVPSVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_015288805.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015288805.2

ORF Insert Sequence:

ATGCAAACTG ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGGTCAG TGAGATGGAA 
CATGAGGATT TTGTAATGGA GGGGCATGGC AAGACTCCAC CTCCTGGTGA AGAAAACAAA 
CAAGAGAAGG AGCAAGAAAG GGAAGAACAG TTAATGGAAG ACAAGAAAAG GAAGAAGGAG 
GATAAGAAAA AGAAGGAATC TGCTCAGAAG GTCACAGATC AAAAAACCAA AGTGCCCGAA 
GTGACGAAAC CAAGTTTAAG CCAACCAGTG GCTGCCAGCC CAATTGGCAA CTCTCCATCG 
CCACCAGTCA ATGGTGGCAA CAATGCCAAA AGGGTGGCAG TGCCGAACGG ACAACCGCCA 
AGCGCCGCCC GCTACATGCC TCGGGAGGTG CCGCCGCGAT TCCGTTGCCA GCAGGACCAC 
AAAGTGTTAC TGAAACGTGG GCAGCCCCCT CCACCATCCT GTATGCTCCT TGGGGGTGGA 
GCAGGGCCTC CTCCCCCTAC AGCACCAGGA GCAAACCCAA ACAACGCACA ACCAGTGACA 
GGAGCACTGC TGCAGAGCGA CAGTGGGACT GCAACAGATT CAACAATTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGT CCTGGAGCCT CCTCCAGCAG CGGCACCAAC 
GCCAACCCAA CTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGTATCG CCAGCAAAGA TGCTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCGAACC CTGGCTTGGA GAAGAACACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGAAGCC AGTGCCAGTC TGGAAGCGCT GGAAATGAAT GTAATCTTGG GGCCTGGAAA 
TCTGATCCCA AGGCTAAATC TGTTCAATCT TCCAACCCTG CTACCGAGAG TAACAGTGGA 
CTAAGTAATT GGAGGAACTT GACTGGACAA GATAGAATTG GTTCTGGTTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCGTCTGCT TGGCCAGCAC TGGTCCAAGA GGGCAATTCT 
AGGAAAGGGA CTTCGGAATC TGATAGTGGT AGCTCCAATG CACAGATTAG CACAGTAGGT 
CAGACCTCTA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAACTT TGTCTCTGGC 
AGAGAACAGG CTCAAATTCA TAACACTGAT GGACCAAAAA ATGGAAACAC TAACTCCTTG 
AACTTAAGTT CACCAAACCC TATGGAGAAT AAGGGAATGC CCTTTGAAAT GGGCTTGGGG 
AACCCTTCCA GGAGCACTGA CACCCCTTCA CAAAGCACTG GAGAAAGAAA GACTGGGAGT 
GTTGGATCTT GGGGTACAGC TAGGGGGTCT TCTGGAACTG ACACAGTCTC TGGACAGAGC 
AATTCTGGAA ACCATGGGAA CAATGGAAAG GATAGGGAGG ACTCCTGGAA AGGAGCTTCT 
GTTCAAAAAC CTAATGGGTC AAGAAGCGAT TCTTGGGATA ACAATAACCG GTCTACGGGT 
GGTGGGTCTT GGAACTTTGG CCCTCAGCAT GCAAATGAAA GCAAATGGGG TGAAGGGAAC 
AAATTAACAT CTGGGGTCTC TCAGGGAGAA TGGAAACAGC TGTCTGGGTC TGATGAACTG 
AAGATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAAAAAGGCC ACCCTCTTCC TGAAAACCAA GGCAATTCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CTGCAGGAAG TGAGGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGACGC CGATCATACA GGCCTACGCA TCCTGATTGC 
CAGGCAGTCT TGCAGACTTT GCTGAGCAGG ACTGATTTGG ACCCCCGAGT GCTTTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAA GATACCGTAT GGGATATTGA AGAGATGCCA 
CGGTCCGAGG GGAAGTCTGA TAAAGGAACT GAGGGGTGGG AGAGCTCTGC CACACAGACA 
AAGAACTCAG GGGGCTGGGG CGATGTACCC AGCCAAAGCA ATCAAATCAA GTCTGGATGG 
GGTGAGCTCT CAACCCCAAC AGAATGGAAG GACCCCAAAA ATACTGGAGG ATGGAATGAC 
TATAAGAACC CCAATTCGTC TAATTGGGGA GGGGGAAGAC AAGAAGAGAA ATCGTCATCT 
TCTTGGAATG ACAGCTCTAG TAAGGATCAG GGGTGGGGTG GACGGCAGCC TAATCAAGGA 
TGGTCTTCTG GAAAGAATGG TTGGGGAGAG GAAGTTGACC AAGCAAAAAA CAGCAACTGG 
GAAAGTGCGG GAAATAAGCC AGCATCTGGT TGGGGTGAAG GTGGGCAAAA TGAGATTGGA 
ACTTGGGGTA ACGGTGCAAA TACAGGCTCT GCTTCAAAGG GTGGTTGGGA TGATTGTAAA 
AGAAATTCAA CGTGGAACGA GACGGGTCGA CAGCCCAACT CCTGGAACAA GCAGCAGCAG 
CAGCAACAGC AGCAACAGCA AGAGACTTCT GGTTCCTGGG GTCCACCGCC ACAAACTCCA 
AGTAATGGGC GACCTCCAAA CCCAAACTGG AACAGCGGAC CACAACCAGC TGCCCCCAAA 
GACGAGGAGC CTAGTGGCTG GGAAGAACCT TCTCCACAGT CAATCAGTCG AAAAATGGAT 
ATTGATGATG GCACTTCAGC GTGGGGAGAT CCTAGCAGTT ATAACTACAA GAATGTGAAC 
CTGTGGGACA AGAACTCACA AGGGGGACAG GCCCCACGGG AACAGAGCCT GCCTACTCCA 
ATGACTAGCA AATCACAAGC ATCAGTCTGG AGCAAAAGCA CTCCACCTGC TCCAGATAAT 
GGTACGTCCG CCTGGGGTGA GCCAAATGAA ACCAGTCCCG GGTGGGGTGA AGTAGATGAT 
ACAGGAGCAT CGACAACAGG CTGGGGGAAT GCACCTTCCA ACGCCCCGAA TGCCATGAAA 
TCTAGTTCTA AATCTATGCA AGATGGCTGG GGAGAGAGTG ATGGGCCAGT GACAGGAACA 
CGTCATTCCA GCTGGGAAGA GGAGGAGGAG GGAGGAGTCT GGAACACAGC AGGCTCTCAG 
GGAAGCAGTT CCTCCCACAA CTCAACAAGC TGGGGGCAAG GAGGAAAGAA AACGCAGATG 
AAGTGCTCGT TAAAAGGAGG AAATAGTGAT TCATGGATGA ACCCTATATC CAAGCAGTTT 
TCAAATATGG GCTTGCTAAG TCAAACTGAG GACATTCCAG GCAATAAGAT GGACTTGTCT 
GTAGGTGGTC TCCCAGATAA GAAGTTTGAG GTAGATAAAC GAGCCATGAA TCTCGGGGAT 
TTTAATGACA TAATGAGAAA GGATCGATCT GGGTTCCGTC CACCTAATTC CAAAGACATG 
GGAACCACAG ATAGTGGGCC TTATTTTGAG AAGGGTGGCA ATCATGGTTT GTTTGGAAAC 
AGCACAGCAC AATCGAGGGG CATGCACACA CCAGTGCAGC CACTAAATCC TTCCCCCAAT 
ATCCGGGCGC AAGTGCCTCC CCAATTTCTT TCTCCCCAGG TTTCGGCCTC CATGCTCAAA 
CAGTTCCCCA ACAGTGGCTT GAATCCAGGT CTTTTCAATG TGGGGCCCCA GCTTTCTCCA 
CAACAGATTG CCATGCTGAG CCAGCTTCCA CAGATTCCCC AGTTTCAATT AGCATGTCAG 
CTCCTCCTTC AGCAGCAGCA GCAGCAGCAG CAGCTGTTAC AGAGCCAGAG GAAGTTATCT 
CAAGCTGTGC GACAGCAGCA GGAGCAACAG ATTGCTCGTA TGGTGAGTGC CCTCCAGCAG 
CAGCAGCAGA GGCAGCCTGG CATGAAGCAT TCACCATCCC ACCCTGTTGG GCCTAAGCCA 
CATATAGACA GCATGGTACC CAATCCTTTG AATGTGGGTC TTTCAGATTT ACAGACCAAA 
GGACAAATAC CTGGATACGG TTCAGGCTTT GGCTCAAGCA GCATGGATTA TGGCATGGTG 
GGAGGGAAAG AAGCTGGAAC AGAATCCCGC TTTAAACAGT GGACTATGAT GGAAGGGCTG 
CCCTCTGTGG CTTCACAAGA TGCCAATATG CACAAAAATG GTGCAATAGT GCCCCCTGGA 
AAGGCTCGCG GAGGATCGCC CTACAACCAG TTCGACATAA TTCAGGGCGA CCCACTAGGT 
GGCCATGCAG GCCCTGCTGG TGATAGTTGG TTACCTGCCA AATCTCCACC GACAAACAAG 
ATCGGAAGCA AATCCAGCAA CGCCAGCTGG CCTCCAGAGT TCCAACCAGG AGTGCCATGG 
AAAGGTATAC AAAACATTGA CCCTGAATCT GACCCCTACG TGACCCCTGG AAGTGTGCTG 
GGGGGGACAG CCACATCTCC CATTGTAGAT ACTGACCACC AACTTCTGCG GGACAACACC 
ACAGGGTCTA ATTCTTCCCT CAACACCTCG CTGCCTTCAC CTGGTGCCTG GCCCTACAGT 
GCCTCTGACA ATTCCTTCAC CAACGTTCAT AGCACTTCAG CAAAATTCAG TGATTACAAA 
TCAACATGGT CCCCAGATCC CATAGGACAC AATCCCACTC ATCTCTCCAA CAAGATGTGG 
AAAAACCATA TTTCCTCAAG GAACACTACA GGGCTGCCTC GCCCGCCTCC TGGCCTGACC 
AACCCCAAAC CATCATCTCC ATGGAACAGC ACAGCACCCC GATCGGTCAG GGGCTGGGGG 
GCACAGGACT CAAGGCTTGC CTCTGCATCT ACTTGGAGTG ATGGTAGCTC AGTTCGTCCT 
AGCTACTGGC TGGTTCTTCA CAATCTCACT CCACAGATCG ATGGGTCAAC CTTGAGGACG 
ATCTGCATGC AGCATGGTCC ACTGCTGACA TTCCATCTGA ACCTGACCCA GGGCACCGCT 
CTCATCCGAT ACAACACCAA ACAGGAGGCG GCCAAGGCCC AGACTGCACT GCACATGTGT 
GTGTTGGGAA ACACTACCAT CCTTGCTGAG TTTGCCACAG ATGAAGAGGT TAGTCGCTTT 
TTGACACAAG CTCAGCCCCC TACACCTGCA GCAACCCCAA GTGCACCCAC GGCAGGCTGG 
CAATCCCTGG AGACAGGACA GAACCAGACA GATCCAGTTG GACCTGCTTT GAATCTCTTT 
GGTGGGTCAA CAGGGCTTGG GCAGTGGAGC AGTGGTGGCG GCGGCAGCAG TGGGGGTGAT 
CTCACTGGCG CTTCGTTGTG GGGGCCCCCA AACTATTCTT CAAGCTTGTG GGGGGTCCCA 
AGCGTAGAGG ATCCACACAG AATGGGCAGC CCTGCTCCCT TACTACCTGG AGACCTTCTG 
GGAGGAGGGT CGGATTCAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_015288805.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015144291.1 Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X7, mRNA.

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CloneID	OGa29595
Clone ID Related Accession (Same CDS sequence)	XM_015288805.2 , XM_015288805.1
Accession Version	XM_015288805.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	trinucleotide repeat-containing gene 6B protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455714.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACTG ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGGTCAG TGAGATGGAA 
CATGAGGATT TTGTAATGGA GGGGCATGGC AAGACTCCAC CTCCTGGTGA AGAAAACAAA 
CAAGAGAAGG AGCAAGAAAG GGAAGAACAG TTAATGGAAG ACAAGAAAAG GAAGAAGGAG 
GATAAGAAAA AGAAGGAATC TGCTCAGAAG GTCACAGATC AAAAAACCAA AGTGCCCGAA 
GTGACGAAAC CAAGTTTAAG CCAACCAGTG GCTGCCAGCC CAATTGGCAA CTCTCCATCG 
CCACCAGTCA ATGGTGGCAA CAATGCCAAA AGGGTGGCAG TGCCGAACGG ACAACCGCCA 
AGCGCCGCCC GCTACATGCC TCGGGAGGTG CCGCCGCGAT TCCGTTGCCA GCAGGACCAC 
AAAGTGTTAC TGAAACGTGG GCAGCCCCCT CCACCATCCT GTATGCTCCT TGGGGGTGGA 
GCAGGGCCTC CTCCCCCTAC AGCACCAGGA GCAAACCCAA ACAACGCACA ACCAGTGACA 
GGAGCACTGC TGCAGAGCGA CAGTGGGACT GCAACAGATT CAACAATTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGT CCTGGAGCCT CCTCCAGCAG CGGCACCAAC 
GCCAACCCAA CTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGTATCG CCAGCAAAGA TGCTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCGAACC CTGGCTTGGA GAAGAACACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGAAGCC AGTGCCAGTC TGGAAGCGCT GGAAATGAAT GTAATCTTGG GGCCTGGAAA 
TCTGATCCCA AGGCTAAATC TGTTCAATCT TCCAACCCTG CTACCGAGAG TAACAGTGGA 
CTAAGTAATT GGAGGAACTT GACTGGACAA GATAGAATTG GTTCTGGTTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCGTCTGCT TGGCCAGCAC TGGTCCAAGA GGGCAATTCT 
AGGAAAGGGA CTTCGGAATC TGATAGTGGT AGCTCCAATG CACAGATTAG CACAGTAGGT 
CAGACCTCTA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAACTT TGTCTCTGGC 
AGAGAACAGG CTCAAATTCA TAACACTGAT GGACCAAAAA ATGGAAACAC TAACTCCTTG 
AACTTAAGTT CACCAAACCC TATGGAGAAT AAGGGAATGC CCTTTGAAAT GGGCTTGGGG 
AACCCTTCCA GGAGCACTGA CACCCCTTCA CAAAGCACTG GAGAAAGAAA GACTGGGAGT 
GTTGGATCTT GGGGTACAGC TAGGGGGTCT TCTGGAACTG ACACAGTCTC TGGACAGAGC 
AATTCTGGAA ACCATGGGAA CAATGGAAAG GATAGGGAGG ACTCCTGGAA AGGAGCTTCT 
GTTCAAAAAC CTAATGGGTC AAGAAGCGAT TCTTGGGATA ACAATAACCG GTCTACGGGT 
GGTGGGTCTT GGAACTTTGG CCCTCAGCAT GCAAATGAAA GCAAATGGGG TGAAGGGAAC 
AAATTAACAT CTGGGGTCTC TCAGGGAGAA TGGAAACAGC TGTCTGGGTC TGATGAACTG 
AAGATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAAAAAGGCC ACCCTCTTCC TGAAAACCAA GGCAATTCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CTGCAGGAAG TGAGGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGACGC CGATCATACA GGCCTACGCA TCCTGATTGC 
CAGGCAGTCT TGCAGACTTT GCTGAGCAGG ACTGATTTGG ACCCCCGAGT GCTTTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAA GATACCGTAT GGGATATTGA AGAGATGCCA 
CGGTCCGAGG GGAAGTCTGA TAAAGGAACT GAGGGGTGGG AGAGCTCTGC CACACAGACA 
AAGAACTCAG GGGGCTGGGG CGATGTACCC AGCCAAAGCA ATCAAATCAA GTCTGGATGG 
GGTGAGCTCT CAACCCCAAC AGAATGGAAG GACCCCAAAA ATACTGGAGG ATGGAATGAC 
TATAAGAACC CCAATTCGTC TAATTGGGGA GGGGGAAGAC AAGAAGAGAA ATCGTCATCT 
TCTTGGAATG ACAGCTCTAG TAAGGATCAG GGGTGGGGTG GACGGCAGCC TAATCAAGGA 
TGGTCTTCTG GAAAGAATGG TTGGGGAGAG GAAGTTGACC AAGCAAAAAA CAGCAACTGG 
GAAAGTGCGG GAAATAAGCC AGCATCTGGT TGGGGTGAAG GTGGGCAAAA TGAGATTGGA 
ACTTGGGGTA ACGGTGCAAA TACAGGCTCT GCTTCAAAGG GTGGTTGGGA TGATTGTAAA 
AGAAATTCAA CGTGGAACGA GACGGGTCGA CAGCCCAACT CCTGGAACAA GCAGCAGCAG 
CAGCAACAGC AGCAACAGCA AGAGACTTCT GGTTCCTGGG GTCCACCGCC ACAAACTCCA 
AGTAATGGGC GACCTCCAAA CCCAAACTGG AACAGCGGAC CACAACCAGC TGCCCCCAAA 
GACGAGGAGC CTAGTGGCTG GGAAGAACCT TCTCCACAGT CAATCAGTCG AAAAATGGAT 
ATTGATGATG GCACTTCAGC GTGGGGAGAT CCTAGCAGTT ATAACTACAA GAATGTGAAC 
CTGTGGGACA AGAACTCACA AGGGGGACAG GCCCCACGGG AACAGAGCCT GCCTACTCCA 
ATGACTAGCA AATCACAAGC ATCAGTCTGG AGCAAAAGCA CTCCACCTGC TCCAGATAAT 
GGTACGTCCG CCTGGGGTGA GCCAAATGAA ACCAGTCCCG GGTGGGGTGA AGTAGATGAT 
ACAGGAGCAT CGACAACAGG CTGGGGGAAT GCACCTTCCA ACGCCCCGAA TGCCATGAAA 
TCTAGTTCTA AATCTATGCA AGATGGCTGG GGAGAGAGTG ATGGGCCAGT GACAGGAACA 
CGTCATTCCA GCTGGGAAGA GGAGGAGGAG GGAGGAGTCT GGAACACAGC AGGCTCTCAG 
GGAAGCAGTT CCTCCCACAA CTCAACAAGC TGGGGGCAAG GAGGAAAGAA AACGCAGATG 
AAGTGCTCGT TAAAAGGAGG AAATAGTGAT TCATGGATGA ACCCTATATC CAAGCAGTTT 
TCAAATATGG GCTTGCTAAG TCAAACTGAG GACATTCCAG GCAATAAGAT GGACTTGTCT 
GTAGGTGGTC TCCCAGATAA GAAGTTTGAG GTAGATAAAC GAGCCATGAA TCTCGGGGAT 
TTTAATGACA TAATGAGAAA GGATCGATCT GGGTTCCGTC CACCTAATTC CAAAGACATG 
GGAACCACAG ATAGTGGGCC TTATTTTGAG AAGGGTGGCA ATCATGGTTT GTTTGGAAAC 
AGCACAGCAC AATCGAGGGG CATGCACACA CCAGTGCAGC CACTAAATCC TTCCCCCAAT 
ATCCGGGCGC AAGTGCCTCC CCAATTTCTT TCTCCCCAGG TTTCGGCCTC CATGCTCAAA 
CAGTTCCCCA ACAGTGGCTT GAATCCAGGT CTTTTCAATG TGGGGCCCCA GCTTTCTCCA 
CAACAGATTG CCATGCTGAG CCAGCTTCCA CAGATTCCCC AGTTTCAATT AGCATGTCAG 
CTCCTCCTTC AGCAGCAGCA GCAGCAGCAG CAGCTGTTAC AGAGCCAGAG GAAGTTATCT 
CAAGCTGTGC GACAGCAGCA GGAGCAACAG ATTGCTCGTA TGGTGAGTGC CCTCCAGCAG 
CAGCAGCAGA GGCAGCCTGG CATGAAGCAT TCACCATCCC ACCCTGTTGG GCCTAAGCCA 
CATATAGACA GCATGGTACC CAATCCTTTG AATGTGGGTC TTTCAGATTT ACAGACCAAA 
GGACAAATAC CTGGATACGG TTCAGGCTTT GGCTCAAGCA GCATGGATTA TGGCATGGTG 
GGAGGGAAAG AAGCTGGAAC AGAATCCCGC TTTAAACAGT GGACTATGAT GGAAGGGCTG 
CCCTCTGTGG CTTCACAAGA TGCCAATATG CACAAAAATG GTGCAATAGT GCCCCCTGGA 
AAGGCTCGCG GAGGATCGCC CTACAACCAG TTCGACATAA TTCAGGGCGA CCCACTAGGT 
GGCCATGCAG GCCCTGCTGG TGATAGTTGG TTACCTGCCA AATCTCCACC GACAAACAAG 
ATCGGAAGCA AATCCAGCAA CGCCAGCTGG CCTCCAGAGT TCCAACCAGG AGTGCCATGG 
AAAGGTATAC AAAACATTGA CCCTGAATCT GACCCCTACG TGACCCCTGG AAGTGTGCTG 
GGGGGGACAG CCACATCTCC CATTGTAGAT ACTGACCACC AACTTCTGCG GGACAACACC 
ACAGGGTCTA ATTCTTCCCT CAACACCTCG CTGCCTTCAC CTGGTGCCTG GCCCTACAGT 
GCCTCTGACA ATTCCTTCAC CAACGTTCAT AGCACTTCAG CAAAATTCAG TGATTACAAA 
TCAACATGGT CCCCAGATCC CATAGGACAC AATCCCACTC ATCTCTCCAA CAAGATGTGG 
AAAAACCATA TTTCCTCAAG GAACACTACA GGGCTGCCTC GCCCGCCTCC TGGCCTGACC 
AACCCCAAAC CATCATCTCC ATGGAACAGC ACAGCACCCC GATCGGTCAG GGGCTGGGGG 
GCACAGGACT CAAGGCTTGC CTCTGCATCT ACTTGGAGTG ATGGTAGCTC AGTTCGTCCT 
AGCTACTGGC TGGTTCTTCA CAATCTCACT CCACAGATCG ATGGGTCAAC CTTGAGGACG 
ATCTGCATGC AGCATGGTCC ACTGCTGACA TTCCATCTGA ACCTGACCCA GGGCACCGCT 
CTCATCCGAT ACAACACCAA ACAGGAGGCG GCCAAGGCCC AGACTGCACT GCACATGTGT 
GTGTTGGGAA ACACTACCAT CCTTGCTGAG TTTGCCACAG ATGAAGAGGT TAGTCGCTTT 
TTGACACAAG CTCAGCCCCC TACACCTGCA GCAACCCCAA GTGCACCCAC GGCAGGCTGG 
CAATCCCTGG AGACAGGACA GAACCAGACA GATCCAGTTG GACCTGCTTT GAATCTCTTT 
GGTGGGTCAA CAGGGCTTGG GCAGTGGAGC AGTGGTGGCG GCGGCAGCAG TGGGGGTGAT 
CTCACTGGCG CTTCGTTGTG GGGGCCCCCA AACTATTCTT CAAGCTTGTG GGGGGTCCCA 
AGCGTAGAGG ATCCACACAG AATGGGCAGC CCTGCTCCCT TACTACCTGG AGACCTTCTG 
GGAGGAGGGT CGGATTCAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015144291.1
CDS	535..5958
Translation	MQTDEGEILEEGSPKVSEMEHEDFVMEGHGKTPPPGEENKQEKEQEREEQLMEDKKRKKEDKKKKESAQKVTDQKTKVPEVTKPSLSQPVAASPIGNSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPPTAPGANPNNAQPVTGALLQSDSGTATDSTIGGAAASNYANSTWGPGASSSSGTNANPTHIWDKVIVDGSDMEEWPCIASKDAESSSENTTDNNSASNPGLEKNTLPGSTTSNKGKGSQCQSGSAGNECNLGAWKSDPKAKSVQSSNPATESNSGLSNWRNLTGQDRIGSGSGFSNFNPNSNPSAWPALVQEGNSRKGTSESDSGSSNAQISTVGQTSREQQSKMENAGVNFVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFEMGLGNPSRSTDTPSQSTGERKTGSVGSWGTARGSSGTDTVSGQSNSGNHGNNGKDREDSWKGASVQKPNGSRSDSWDNNNRSTGGGSWNFGPQHANESKWGEGNKLTSGVSQGEWKQLSGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNSQAPCWGRSSSSAGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEMPRSEGKSDKGTEGWESSATQTKNSGGWGDVPSQSNQIKSGWGELSTPTEWKDPKNTGGWNDYKNPNSSNWGGGRQEEKSSSSWNDSSSKDQGWGGRQPNQGWSSGKNGWGEEVDQAKNSNWESAGNKPASGWGEGGQNEIGTWGNGANTGSASKGGWDDCKRNSTWNETGRQPNSWNKQQQQQQQQQQETSGSWGPPPQTPSNGRPPNPNWNSGPQPAAPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPSSYNYKNVNLWDKNSQGGQAPREQSLPTPMTSKSQASVWSKSTPPAPDNGTSAWGEPNETSPGWGEVDDTGASTTGWGNAPSNAPNAMKSSSKSMQDGWGESDGPVTGTRHSSWEEEEEGGVWNTAGSQGSSSSHNSTSWGQGGKKTQMKCSLKGGNSDSWMNPISKQFSNMGLLSQTEDIPGNKMDLSVGGLPDKKFEVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGMHTPVQPLNPSPNIRAQVPPQFLSPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQQLLQSQRKLSQAVRQQQEQQIARMVSALQQQQQRQPGMKHSPSHPVGPKPHIDSMVPNPLNVGLSDLQTKGQIPGYGSGFGSSSMDYGMVGGKEAGTESRFKQWTMMEGLPSVASQDANMHKNGAIVPPGKARGGSPYNQFDIIQGDPLGGHAGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFSDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTGLPRPPPGLTNPKPSSPWNSTAPRSVRGWGAQDSRLASASTWSDGSSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYNTKQEAAKAQTALHMCVLGNTTILAEFATDEEVSRFLTQAQPPTPAATPSAPTAGWQSLETGQNQTDPVGPALNLFGGSTGLGQWSSGGGGSSGGDLTGASLWGPPNYSSSLWGVPSVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_015288805.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015288805.1

ORF Insert Sequence:

ATGCAAACTG ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGGTCAG TGAGATGGAA 
CATGAGGATT TTGTAATGGA GGGGCATGGC AAGACTCCAC CTCCTGGTGA AGAAAACAAA 
CAAGAGAAGG AGCAAGAAAG GGAAGAACAG TTAATGGAAG ACAAGAAAAG GAAGAAGGAG 
GATAAGAAAA AGAAGGAATC TGCTCAGAAG GTCACAGATC AAAAAACCAA AGTGCCCGAA 
GTGACGAAAC CAAGTTTAAG CCAACCAGTG GCTGCCAGCC CAATTGGCAA CTCTCCATCG 
CCACCAGTCA ATGGTGGCAA CAATGCCAAA AGGGTGGCAG TGCCGAACGG ACAACCGCCA 
AGCGCCGCCC GCTACATGCC TCGGGAGGTG CCGCCGCGAT TCCGTTGCCA GCAGGACCAC 
AAAGTGTTAC TGAAACGTGG GCAGCCCCCT CCACCATCCT GTATGCTCCT TGGGGGTGGA 
GCAGGGCCTC CTCCCCCTAC AGCACCAGGA GCAAACCCAA ACAACGCACA ACCAGTGACA 
GGAGCACTGC TGCAGAGCGA CAGTGGGACT GCAACAGATT CAACAATTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGT CCTGGAGCCT CCTCCAGCAG CGGCACCAAC 
GCCAACCCAA CTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGTATCG CCAGCAAAGA TGCTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCGAACC CTGGCTTGGA GAAGAACACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGAAGCC AGTGCCAGTC TGGAAGCGCT GGAAATGAAT GTAATCTTGG GGCCTGGAAA 
TCTGATCCCA AGGCTAAATC TGTTCAATCT TCCAACCCTG CTACCGAGAG TAACAGTGGA 
CTAAGTAATT GGAGGAACTT GACTGGACAA GATAGAATTG GTTCTGGTTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCGTCTGCT TGGCCAGCAC TGGTCCAAGA GGGCAATTCT 
AGGAAAGGGA CTTCGGAATC TGATAGTGGT AGCTCCAATG CACAGATTAG CACAGTAGGT 
CAGACCTCTA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAACTT TGTCTCTGGC 
AGAGAACAGG CTCAAATTCA TAACACTGAT GGACCAAAAA ATGGAAACAC TAACTCCTTG 
AACTTAAGTT CACCAAACCC TATGGAGAAT AAGGGAATGC CCTTTGAAAT GGGCTTGGGG 
AACCCTTCCA GGAGCACTGA CACCCCTTCA CAAAGCACTG GAGAAAGAAA GACTGGGAGT 
GTTGGATCTT GGGGTACAGC TAGGGGGTCT TCTGGAACTG ACACAGTCTC TGGACAGAGC 
AATTCTGGAA ACCATGGGAA CAATGGAAAG GATAGGGAGG ACTCCTGGAA AGGAGCTTCT 
GTTCAAAAAC CTAATGGGTC AAGAAGCGAT TCTTGGGATA ACAATAACCG GTCTACGGGT 
GGTGGGTCTT GGAACTTTGG CCCTCAGCAT GCAAATGAAA GCAAATGGGG TGAAGGGAAC 
AAATTAACAT CTGGGGTCTC TCAGGGAGAA TGGAAACAGC TGTCTGGGTC TGATGAACTG 
AAGATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAAAAAGGCC ACCCTCTTCC TGAAAACCAA GGCAATTCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CTGCAGGAAG TGAGGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGACGC CGATCATACA GGCCTACGCA TCCTGATTGC 
CAGGCAGTCT TGCAGACTTT GCTGAGCAGG ACTGATTTGG ACCCCCGAGT GCTTTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAA GATACCGTAT GGGATATTGA AGAGATGCCA 
CGGTCCGAGG GGAAGTCTGA TAAAGGAACT GAGGGGTGGG AGAGCTCTGC CACACAGACA 
AAGAACTCAG GGGGCTGGGG CGATGTACCC AGCCAAAGCA ATCAAATCAA GTCTGGATGG 
GGTGAGCTCT CAACCCCAAC AGAATGGAAG GACCCCAAAA ATACTGGAGG ATGGAATGAC 
TATAAGAACC CCAATTCGTC TAATTGGGGA GGGGGAAGAC AAGAAGAGAA ATCGTCATCT 
TCTTGGAATG ACAGCTCTAG TAAGGATCAG GGGTGGGGTG GACGGCAGCC TAATCAAGGA 
TGGTCTTCTG GAAAGAATGG TTGGGGAGAG GAAGTTGACC AAGCAAAAAA CAGCAACTGG 
GAAAGTGCGG GAAATAAGCC AGCATCTGGT TGGGGTGAAG GTGGGCAAAA TGAGATTGGA 
ACTTGGGGTA ACGGTGCAAA TACAGGCTCT GCTTCAAAGG GTGGTTGGGA TGATTGTAAA 
AGAAATTCAA CGTGGAACGA GACGGGTCGA CAGCCCAACT CCTGGAACAA GCAGCAGCAG 
CAGCAACAGC AGCAACAGCA AGAGACTTCT GGTTCCTGGG GTCCACCGCC ACAAACTCCA 
AGTAATGGGC GACCTCCAAA CCCAAACTGG AACAGCGGAC CACAACCAGC TGCCCCCAAA 
GACGAGGAGC CTAGTGGCTG GGAAGAACCT TCTCCACAGT CAATCAGTCG AAAAATGGAT 
ATTGATGATG GCACTTCAGC GTGGGGAGAT CCTAGCAGTT ATAACTACAA GAATGTGAAC 
CTGTGGGACA AGAACTCACA AGGGGGACAG GCCCCACGGG AACAGAGCCT GCCTACTCCA 
ATGACTAGCA AATCACAAGC ATCAGTCTGG AGCAAAAGCA CTCCACCTGC TCCAGATAAT 
GGTACGTCCG CCTGGGGTGA GCCAAATGAA ACCAGTCCCG GGTGGGGTGA AGTAGATGAT 
ACAGGAGCAT CGACAACAGG CTGGGGGAAT GCACCTTCCA ACGCCCCGAA TGCCATGAAA 
TCTAGTTCTA AATCTATGCA AGATGGCTGG GGAGAGAGTG ATGGGCCAGT GACAGGAACA 
CGTCATTCCA GCTGGGAAGA GGAGGAGGAG GGAGGAGTCT GGAACACAGC AGGCTCTCAG 
GGAAGCAGTT CCTCCCACAA CTCAACAAGC TGGGGGCAAG GAGGAAAGAA AACGCAGATG 
AAGTGCTCGT TAAAAGGAGG AAATAGTGAT TCATGGATGA ACCCTATATC CAAGCAGTTT 
TCAAATATGG GCTTGCTAAG TCAAACTGAG GACATTCCAG GCAATAAGAT GGACTTGTCT 
GTAGGTGGTC TCCCAGATAA GAAGTTTGAG GTAGATAAAC GAGCCATGAA TCTCGGGGAT 
TTTAATGACA TAATGAGAAA GGATCGATCT GGGTTCCGTC CACCTAATTC CAAAGACATG 
GGAACCACAG ATAGTGGGCC TTATTTTGAG AAGGGTGGCA ATCATGGTTT GTTTGGAAAC 
AGCACAGCAC AATCGAGGGG CATGCACACA CCAGTGCAGC CACTAAATCC TTCCCCCAAT 
ATCCGGGCGC AAGTGCCTCC CCAATTTCTT TCTCCCCAGG TTTCGGCCTC CATGCTCAAA 
CAGTTCCCCA ACAGTGGCTT GAATCCAGGT CTTTTCAATG TGGGGCCCCA GCTTTCTCCA 
CAACAGATTG CCATGCTGAG CCAGCTTCCA CAGATTCCCC AGTTTCAATT AGCATGTCAG 
CTCCTCCTTC AGCAGCAGCA GCAGCAGCAG CAGCTGTTAC AGAGCCAGAG GAAGTTATCT 
CAAGCTGTGC GACAGCAGCA GGAGCAACAG ATTGCTCGTA TGGTGAGTGC CCTCCAGCAG 
CAGCAGCAGA GGCAGCCTGG CATGAAGCAT TCACCATCCC ACCCTGTTGG GCCTAAGCCA 
CATATAGACA GCATGGTACC CAATCCTTTG AATGTGGGTC TTTCAGATTT ACAGACCAAA 
GGACAAATAC CTGGATACGG TTCAGGCTTT GGCTCAAGCA GCATGGATTA TGGCATGGTG 
GGAGGGAAAG AAGCTGGAAC AGAATCCCGC TTTAAACAGT GGACTATGAT GGAAGGGCTG 
CCCTCTGTGG CTTCACAAGA TGCCAATATG CACAAAAATG GTGCAATAGT GCCCCCTGGA 
AAGGCTCGCG GAGGATCGCC CTACAACCAG TTCGACATAA TTCAGGGCGA CCCACTAGGT 
GGCCATGCAG GCCCTGCTGG TGATAGTTGG TTACCTGCCA AATCTCCACC GACAAACAAG 
ATCGGAAGCA AATCCAGCAA CGCCAGCTGG CCTCCAGAGT TCCAACCAGG AGTGCCATGG 
AAAGGTATAC AAAACATTGA CCCTGAATCT GACCCCTACG TGACCCCTGG AAGTGTGCTG 
GGGGGGACAG CCACATCTCC CATTGTAGAT ACTGACCACC AACTTCTGCG GGACAACACC 
ACAGGGTCTA ATTCTTCCCT CAACACCTCG CTGCCTTCAC CTGGTGCCTG GCCCTACAGT 
GCCTCTGACA ATTCCTTCAC CAACGTTCAT AGCACTTCAG CAAAATTCAG TGATTACAAA 
TCAACATGGT CCCCAGATCC CATAGGACAC AATCCCACTC ATCTCTCCAA CAAGATGTGG 
AAAAACCATA TTTCCTCAAG GAACACTACA GGGCTGCCTC GCCCGCCTCC TGGCCTGACC 
AACCCCAAAC CATCATCTCC ATGGAACAGC ACAGCACCCC GATCGGTCAG GGGCTGGGGG 
GCACAGGACT CAAGGCTTGC CTCTGCATCT ACTTGGAGTG ATGGTAGCTC AGTTCGTCCT 
AGCTACTGGC TGGTTCTTCA CAATCTCACT CCACAGATCG ATGGGTCAAC CTTGAGGACG 
ATCTGCATGC AGCATGGTCC ACTGCTGACA TTCCATCTGA ACCTGACCCA GGGCACCGCT 
CTCATCCGAT ACAACACCAA ACAGGAGGCG GCCAAGGCCC AGACTGCACT GCACATGTGT 
GTGTTGGGAA ACACTACCAT CCTTGCTGAG TTTGCCACAG ATGAAGAGGT TAGTCGCTTT 
TTGACACAAG CTCAGCCCCC TACACCTGCA GCAACCCCAA GTGCACCCAC GGCAGGCTGG 
CAATCCCTGG AGACAGGACA GAACCAGACA GATCCAGTTG GACCTGCTTT GAATCTCTTT 
GGTGGGTCAA CAGGGCTTGG GCAGTGGAGC AGTGGTGGCG GCGGCAGCAG TGGGGGTGAT 
CTCACTGGCG CTTCGTTGTG GGGGCCCCCA AACTATTCTT CAAGCTTGTG GGGGGTCCCA 
AGCGTAGAGG ATCCACACAG AATGGGCAGC CCTGCTCCCT TACTACCTGG AGACCTTCTG 
GGAGGAGGGT CGGATTCAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_015288813.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015144299.1 Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X8, mRNA.

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CloneID	OGa29596
Clone ID Related Accession (Same CDS sequence)	XM_015288813.1 , XM_015288813.2
Accession Version	XM_015288813.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	trinucleotide repeat-containing gene 6B protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455714.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACTG ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGGTCAG TGAGATGGAA 
CATGAGGATT TTGTAATGGA GGGGCATGGC AAGACTCCAC CTCCTGGTGA AGAAAACAAA 
CAAGAGAAGG AGCAAGAAAG GGAAGAACAG TTAATGGAAG ACAAGAAAAG GAAGAAGGAG 
GATAAGAAAA AGAAGGAATC TGCTCAGAAG GTCACAGATC AAAAAACCAA AGTGCCCGAA 
GTGACGAAAC CAAGTTTAAG CCAACCAGTG GCTGCCAGCC CAATTGGCAA CTCTCCATCG 
CCACCAGTCA ATGGTGGCAA CAATGCCAAA AGGGTGGCAG TGCCGAACGG ACAACCGCCA 
AGCGCCGCCC GCTACATGCC TCGGGAGGTG CCGCCGCGAT TCCGTTGCCA GCAGGACCAC 
AAAGTGTTAC TGAAACGTGG GCAGCCCCCT CCACCATCCT GTATGCTCCT TGGGGGTGGA 
GCAGGGCCTC CTCCCCCTAC AGCACCAGGA GCAAACCCAA ACAACGCACA ACCAGTGACA 
GGAGCACTGC TGCAGAGCGA CAGTGGGACT GCAACAGATT CAACAATTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGT CCTGGAGCCT CCTCCAGCAG CGGCACCAAC 
GCCAACCCAA CTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGTATCG CCAGCAAAGA TGCTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCGAACC CTGGCTTGGA GAAGAACACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGAAGCC AGTGCCAGTC TGGAAGCGCT GGAAATGAAT GTAATCTTGG GGCCTGGAAA 
TCTGATCCCA AGGCTAAATC TGTTCAATCT TCCAACCCTG CTACCGAGAG TAACAGTGGA 
CTAAGTAATT GGAGGAACTT GACTGGACAA GATAGAATTG GTTCTGGTTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCGTCTGCT TGGCCAGCAC TGGTCCAAGA GGGCAATTCT 
AGGAAAGGGA CTTCGGAATC TGATAGTGGT AGCTCCAATG CACAGATTAG CACAGTAGGT 
CAGACCTCTA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAACTT TGTCTCTGGC 
AGAGAACAGG CTCAAATTCA TAACACTGAT GGACCAAAAA ATGGAAACAC TAACTCCTTG 
AACTTAAGTT CACCAAACCC TATGGAGAAT AAGGGAATGC CCTTTGAAAT GGGCTTGGGG 
AACCCTTCCA GGAGCACTGA CACCCCTTCA CAAAGCACTG GAGAAAGAAA GACTGGGAGT 
GTTGGATCTT GGGGTACAGC TAGGGGGTCT TCTGGAACTG ACACAGTCTC TGGACAGAGC 
AATTCTGGAA ACCATGGGAA CAATGGAAAG GATAGGGAGG ACTCCTGGAA AGGAGCTTCT 
GTTCAAAAAC CTAATGGGTC AAGAAGCGAT TCTTGGGATA ACAATAACCG GTCTACGGGT 
GGTGGGTCTT GGAACTTTGG CCCTCAGCAT GCAAATGAAA GCAAATGGGG TGAAGGGAAC 
AAATTAACAT CTGGGGTCTC TCAGGGAGAA TGGAAACAGC TGTCTGGGTC TGATGAACTG 
AAGATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAAAAAGGCC ACCCTCTTCC TGAAAACCAA GGCAATTCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CTGCAGGAAG TGAGGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGACGC CGATCATACA GGCCTACGCA TCCTGATTGC 
CAGGCAGTCT TGCAGACTTT GCTGAGCAGG ACTGATTTGG ACCCCCGAGT GCTTTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAA GATACCGTAT GGGATATTGA AGAGATGCCA 
CGGTCCGAGG GGAAGTCTGA TAAAGGAACT GAGGGGTGGG AGAGCTCTGC CACACAGACA 
AAGAACTCAG GGGGCTGGGG CGATGTACCC AGCCAAAGCA ATCAAATCAA GTCTGGATGG 
GGTGAGCTCT CAACCCCAAC AGAATGGAAG GACCCCAAAA ATACTGGAGG ATGGAATGAC 
TATAAGAACC CCAATTCGTC TAATTGGGGA GGGGGAAGAC AAGAAGAGAA ATCGTCATCT 
TCTTGGAATG ACAGCTCTAG TAAGGATCAG GGGTGGGGTG GACGGCAGCC TAATCAAGGA 
TGGTCTTCTG GAAAGAATGG TTGGGGAGAG GAAGTTGACC AAGCAAAAAA CAGCAACTGG 
GAAAGTGCGG GAAATAAGCC AGCATCTGGT TGGGGTGAAG GTGGGCAAAA TGAGATTGGA 
ACTTGGGGTA ACGGTGCAAA TACAGGCTCT GCTTCAAAGG GTGGTTGGGA TGATTGTAAA 
AGAAATTCAA CGTGGAACGA GACGGGTCGA CAGCCCAACT CCTGGAACAA GCAGCAGCAG 
CAGCAACAGC AGCAACAGCA AGAGACTTCT GGTTCCTGGG GTCCACCGCC ACAAACTCCA 
AGTAATGGGC GACCTCCAAA CCCAAACTGG AACAGCGGAC CACAACCAGC TGCCCCCAAA 
GACGAGGAGC CTAGTGGCTG GGAAGAACCT TCTCCACAGT CAATCAGTCG AAAAATGGAT 
ATTGATGATG GCACTTCAGC GTGGGGAGAT CCTAGCAGTT ATAACTACAA GAATGTGAAC 
CTGTGGGACA AGAACTCACA AGGGGGACAG GCCCCACGGG AACAGAGCCT GCCTACTCCA 
ATGACTAGCA AATCACAAGC ATCAGTCTGG AGCAAAAGCA CTCCACCTGC TCCAGATAAT 
GGTACGTCCG CCTGGGGTGA GCCAAATGAA ACCAGTCCCG GGTGGGGTGA AGTAGATGAT 
ACAGGAGCAT CGACAACAGG CTGGGGGAAT GCACCTTCCA ACGCCCCGAA TGCCATGAAA 
TCTAGTTCTA AATCTATGCA AGATGGCTGG GGAGAGAGTG ATGGGCCAGT GACAGGAACA 
CGTCATTCCA GCTGGGAAGA GGAGGAGGAG GGAGGAGTCT GGAACACAGC AGGCTCTCAG 
GGAAGCAGTT CCTCCCACAA CTCAACAAGC TGGGGGCAAG GAGGAAAGAA AACGCAGATG 
AAGTGCTCGT TAAAAGGAGG AAATAGTGAT TCATGGATGA ACCCTATATC CAAGCAGTTT 
TCAAATATGG GCTTGCTAAG TCAAACTGAG GACATTCCAG GCAATAAGAT GGACTTGTCT 
GTAGGTGGTC TCCCAGATAA GAAGTTTGAG GTAGATAAAC GAGCCATGAA TCTCGGGGAT 
TTTAATGACA TAATGAGAAA GGATCGATCT GGGTTCCGTC CACCTAATTC CAAAGACATG 
GGAACCACAG ATAGTGGGCC TTATTTTGAG AAGGTTTCGG CCTCCATGCT CAAACAGTTC 
CCCAACAGTG GCTTGAATCC AGGTCTTTTC AATGTGGGGC CCCAGCTTTC TCCACAACAG 
ATTGCCATGC TGAGCCAGCT TCCACAGATT CCCCAGTTTC AATTAGCATG TCAGCTCCTC 
CTTCAGCAGC AGCAGCAGCA GCAGCAGCTG TTACAGAGCC AGAGGAAGTT ATCTCAAGCT 
GTGCGACAGC AGCAGGAGCA ACAGATTGCT CGTATGGTGA GTGCCCTCCA GCAGCAGCAG 
CAGAGGCAGC CTGGCATGAA GCATTCACCA TCCCACCCTG TTGGGCCTAA GCCACATATA 
GACAGCATGG TACCCAATCC TTTGAATGTG GGTCTTTCAG ATTTACAGAC CAAAGGACAA 
ATACCTGGAT ACGGTTCAGG CTTTGGCTCA AGCAGCATGG ATTATGGCAT GGTGGGAGGG 
AAAGAAGCTG GAACAGAATC CCGCTTTAAA CAGTGGACTA TGATGGAAGG GCTGCCCTCT 
GTGGCTTCAC AAGATGCCAA TATGCACAAA AATGGTGCAA TAGTGCCCCC TGGAAAGGCT 
CGCGGAGGAT CGCCCTACAA CCAGTTCGAC ATAATTCAGG GCGACCCACT AGGTGGCCAT 
GCAGGCCCTG CTGGTGATAG TTGGTTACCT GCCAAATCTC CACCGACAAA CAAGATCGGA 
AGCAAATCCA GCAACGCCAG CTGGCCTCCA GAGTTCCAAC CAGGAGTGCC ATGGAAAGGT 
ATACAAAACA TTGACCCTGA ATCTGACCCC TACGTGACCC CTGGAAGTGT GCTGGGGGGG 
ACAGCCACAT CTCCCATTGT AGATACTGAC CACCAACTTC TGCGGGACAA CACCACAGGG 
TCTAATTCTT CCCTCAACAC CTCGCTGCCT TCACCTGGTG CCTGGCCCTA CAGTGCCTCT 
GACAATTCCT TCACCAACGT TCATAGCACT TCAGCAAAAT TCAGTGATTA CAAATCAACA 
TGGTCCCCAG ATCCCATAGG ACACAATCCC ACTCATCTCT CCAACAAGAT GTGGAAAAAC 
CATATTTCCT CAAGGAACAC TACAGGGCTG CCTCGCCCGC CTCCTGGCCT GACCAACCCC 
AAACCATCAT CTCCATGGAA CAGCACAGCA CCCCGATCGG TCAGGGGCTG GGGGGCACAG 
GACTCAAGGC TTGCCTCTGG TGAGAAGGAT CATCTGATAA CGCCATTGAT CAGACCTGAG 
GAGTGTGCAT TTTACAGCAC TAATGGTTTG GAGGAGAGAA AGTTGCAGTT GCCTGCATCT 
ACTTGGAGTG ATGGTAGCTC AGTTCGTCCT AGCTACTGGC TGGTTCTTCA CAATCTCACT 
CCACAGATCG ATGGGTCAAC CTTGAGGACG ATCTGCATGC AGCATGGTCC ACTGCTGACA 
TTCCATCTGA ACCTGACCCA GGGCACCGCT CTCATCCGAT ACAACACCAA ACAGGAGGCG 
GCCAAGGCCC AGACTGCACT GCACATGTGT GTGTTGGGAA ACACTACCAT CCTTGCTGAG 
TTTGCCACAG ATGAAGAGGT TAGTCGCTTT TTGACACAAG CTCAGCCCCC TACACCTGCA 
GCAACCCCAA GTGCACCCAC GGCAGGCTGG CAATCCCTGG AGACAGGACA GAACCAGACA 
GATCCAGTTG GACCTGCTTT GAATCTCTTT GGTGGGTCAA CAGGGCTTGG GCAGTGGAGC 
AGTGGTGGCG GCGGCAGCAG TGGGGGTGAT CTCACTGGCG CTTCGTTGTG GGGGCCCCCA 
AACTATTCTT CAAGCTTGTG GGGGGTCCCA AGCGTAGAGG ATCCACACAG AATGGGCAGC 
CCTGCTCCCT TACTACCTGG AGACCTTCTG GGAGGAGGGT CGGATTCAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015144299.1
CDS	538..5931
Translation	MQTDEGEILEEGSPKVSEMEHEDFVMEGHGKTPPPGEENKQEKEQEREEQLMEDKKRKKEDKKKKESAQKVTDQKTKVPEVTKPSLSQPVAASPIGNSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPPTAPGANPNNAQPVTGALLQSDSGTATDSTIGGAAASNYANSTWGPGASSSSGTNANPTHIWDKVIVDGSDMEEWPCIASKDAESSSENTTDNNSASNPGLEKNTLPGSTTSNKGKGSQCQSGSAGNECNLGAWKSDPKAKSVQSSNPATESNSGLSNWRNLTGQDRIGSGSGFSNFNPNSNPSAWPALVQEGNSRKGTSESDSGSSNAQISTVGQTSREQQSKMENAGVNFVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFEMGLGNPSRSTDTPSQSTGERKTGSVGSWGTARGSSGTDTVSGQSNSGNHGNNGKDREDSWKGASVQKPNGSRSDSWDNNNRSTGGGSWNFGPQHANESKWGEGNKLTSGVSQGEWKQLSGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNSQAPCWGRSSSSAGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEMPRSEGKSDKGTEGWESSATQTKNSGGWGDVPSQSNQIKSGWGELSTPTEWKDPKNTGGWNDYKNPNSSNWGGGRQEEKSSSSWNDSSSKDQGWGGRQPNQGWSSGKNGWGEEVDQAKNSNWESAGNKPASGWGEGGQNEIGTWGNGANTGSASKGGWDDCKRNSTWNETGRQPNSWNKQQQQQQQQQQETSGSWGPPPQTPSNGRPPNPNWNSGPQPAAPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPSSYNYKNVNLWDKNSQGGQAPREQSLPTPMTSKSQASVWSKSTPPAPDNGTSAWGEPNETSPGWGEVDDTGASTTGWGNAPSNAPNAMKSSSKSMQDGWGESDGPVTGTRHSSWEEEEEGGVWNTAGSQGSSSSHNSTSWGQGGKKTQMKCSLKGGNSDSWMNPISKQFSNMGLLSQTEDIPGNKMDLSVGGLPDKKFEVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQQLLQSQRKLSQAVRQQQEQQIARMVSALQQQQQRQPGMKHSPSHPVGPKPHIDSMVPNPLNVGLSDLQTKGQIPGYGSGFGSSSMDYGMVGGKEAGTESRFKQWTMMEGLPSVASQDANMHKNGAIVPPGKARGGSPYNQFDIIQGDPLGGHAGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFSDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTGLPRPPPGLTNPKPSSPWNSTAPRSVRGWGAQDSRLASGEKDHLITPLIRPEECAFYSTNGLEERKLQLPASTWSDGSSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYNTKQEAAKAQTALHMCVLGNTTILAEFATDEEVSRFLTQAQPPTPAATPSAPTAGWQSLETGQNQTDPVGPALNLFGGSTGLGQWSSGGGGSSGGDLTGASLWGPPNYSSSLWGVPSVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_015288813.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015288813.1

ORF Insert Sequence:

ATGCAAACTG ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGGTCAG TGAGATGGAA 
CATGAGGATT TTGTAATGGA GGGGCATGGC AAGACTCCAC CTCCTGGTGA AGAAAACAAA 
CAAGAGAAGG AGCAAGAAAG GGAAGAACAG TTAATGGAAG ACAAGAAAAG GAAGAAGGAG 
GATAAGAAAA AGAAGGAATC TGCTCAGAAG GTCACAGATC AAAAAACCAA AGTGCCCGAA 
GTGACGAAAC CAAGTTTAAG CCAACCAGTG GCTGCCAGCC CAATTGGCAA CTCTCCATCG 
CCACCAGTCA ATGGTGGCAA CAATGCCAAA AGGGTGGCAG TGCCGAACGG ACAACCGCCA 
AGCGCCGCCC GCTACATGCC TCGGGAGGTG CCGCCGCGAT TCCGTTGCCA GCAGGACCAC 
AAAGTGTTAC TGAAACGTGG GCAGCCCCCT CCACCATCCT GTATGCTCCT TGGGGGTGGA 
GCAGGGCCTC CTCCCCCTAC AGCACCAGGA GCAAACCCAA ACAACGCACA ACCAGTGACA 
GGAGCACTGC TGCAGAGCGA CAGTGGGACT GCAACAGATT CAACAATTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGT CCTGGAGCCT CCTCCAGCAG CGGCACCAAC 
GCCAACCCAA CTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGTATCG CCAGCAAAGA TGCTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCGAACC CTGGCTTGGA GAAGAACACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGAAGCC AGTGCCAGTC TGGAAGCGCT GGAAATGAAT GTAATCTTGG GGCCTGGAAA 
TCTGATCCCA AGGCTAAATC TGTTCAATCT TCCAACCCTG CTACCGAGAG TAACAGTGGA 
CTAAGTAATT GGAGGAACTT GACTGGACAA GATAGAATTG GTTCTGGTTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCGTCTGCT TGGCCAGCAC TGGTCCAAGA GGGCAATTCT 
AGGAAAGGGA CTTCGGAATC TGATAGTGGT AGCTCCAATG CACAGATTAG CACAGTAGGT 
CAGACCTCTA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAACTT TGTCTCTGGC 
AGAGAACAGG CTCAAATTCA TAACACTGAT GGACCAAAAA ATGGAAACAC TAACTCCTTG 
AACTTAAGTT CACCAAACCC TATGGAGAAT AAGGGAATGC CCTTTGAAAT GGGCTTGGGG 
AACCCTTCCA GGAGCACTGA CACCCCTTCA CAAAGCACTG GAGAAAGAAA GACTGGGAGT 
GTTGGATCTT GGGGTACAGC TAGGGGGTCT TCTGGAACTG ACACAGTCTC TGGACAGAGC 
AATTCTGGAA ACCATGGGAA CAATGGAAAG GATAGGGAGG ACTCCTGGAA AGGAGCTTCT 
GTTCAAAAAC CTAATGGGTC AAGAAGCGAT TCTTGGGATA ACAATAACCG GTCTACGGGT 
GGTGGGTCTT GGAACTTTGG CCCTCAGCAT GCAAATGAAA GCAAATGGGG TGAAGGGAAC 
AAATTAACAT CTGGGGTCTC TCAGGGAGAA TGGAAACAGC TGTCTGGGTC TGATGAACTG 
AAGATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAAAAAGGCC ACCCTCTTCC TGAAAACCAA GGCAATTCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CTGCAGGAAG TGAGGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGACGC CGATCATACA GGCCTACGCA TCCTGATTGC 
CAGGCAGTCT TGCAGACTTT GCTGAGCAGG ACTGATTTGG ACCCCCGAGT GCTTTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAA GATACCGTAT GGGATATTGA AGAGATGCCA 
CGGTCCGAGG GGAAGTCTGA TAAAGGAACT GAGGGGTGGG AGAGCTCTGC CACACAGACA 
AAGAACTCAG GGGGCTGGGG CGATGTACCC AGCCAAAGCA ATCAAATCAA GTCTGGATGG 
GGTGAGCTCT CAACCCCAAC AGAATGGAAG GACCCCAAAA ATACTGGAGG ATGGAATGAC 
TATAAGAACC CCAATTCGTC TAATTGGGGA GGGGGAAGAC AAGAAGAGAA ATCGTCATCT 
TCTTGGAATG ACAGCTCTAG TAAGGATCAG GGGTGGGGTG GACGGCAGCC TAATCAAGGA 
TGGTCTTCTG GAAAGAATGG TTGGGGAGAG GAAGTTGACC AAGCAAAAAA CAGCAACTGG 
GAAAGTGCGG GAAATAAGCC AGCATCTGGT TGGGGTGAAG GTGGGCAAAA TGAGATTGGA 
ACTTGGGGTA ACGGTGCAAA TACAGGCTCT GCTTCAAAGG GTGGTTGGGA TGATTGTAAA 
AGAAATTCAA CGTGGAACGA GACGGGTCGA CAGCCCAACT CCTGGAACAA GCAGCAGCAG 
CAGCAACAGC AGCAACAGCA AGAGACTTCT GGTTCCTGGG GTCCACCGCC ACAAACTCCA 
AGTAATGGGC GACCTCCAAA CCCAAACTGG AACAGCGGAC CACAACCAGC TGCCCCCAAA 
GACGAGGAGC CTAGTGGCTG GGAAGAACCT TCTCCACAGT CAATCAGTCG AAAAATGGAT 
ATTGATGATG GCACTTCAGC GTGGGGAGAT CCTAGCAGTT ATAACTACAA GAATGTGAAC 
CTGTGGGACA AGAACTCACA AGGGGGACAG GCCCCACGGG AACAGAGCCT GCCTACTCCA 
ATGACTAGCA AATCACAAGC ATCAGTCTGG AGCAAAAGCA CTCCACCTGC TCCAGATAAT 
GGTACGTCCG CCTGGGGTGA GCCAAATGAA ACCAGTCCCG GGTGGGGTGA AGTAGATGAT 
ACAGGAGCAT CGACAACAGG CTGGGGGAAT GCACCTTCCA ACGCCCCGAA TGCCATGAAA 
TCTAGTTCTA AATCTATGCA AGATGGCTGG GGAGAGAGTG ATGGGCCAGT GACAGGAACA 
CGTCATTCCA GCTGGGAAGA GGAGGAGGAG GGAGGAGTCT GGAACACAGC AGGCTCTCAG 
GGAAGCAGTT CCTCCCACAA CTCAACAAGC TGGGGGCAAG GAGGAAAGAA AACGCAGATG 
AAGTGCTCGT TAAAAGGAGG AAATAGTGAT TCATGGATGA ACCCTATATC CAAGCAGTTT 
TCAAATATGG GCTTGCTAAG TCAAACTGAG GACATTCCAG GCAATAAGAT GGACTTGTCT 
GTAGGTGGTC TCCCAGATAA GAAGTTTGAG GTAGATAAAC GAGCCATGAA TCTCGGGGAT 
TTTAATGACA TAATGAGAAA GGATCGATCT GGGTTCCGTC CACCTAATTC CAAAGACATG 
GGAACCACAG ATAGTGGGCC TTATTTTGAG AAGGTTTCGG CCTCCATGCT CAAACAGTTC 
CCCAACAGTG GCTTGAATCC AGGTCTTTTC AATGTGGGGC CCCAGCTTTC TCCACAACAG 
ATTGCCATGC TGAGCCAGCT TCCACAGATT CCCCAGTTTC AATTAGCATG TCAGCTCCTC 
CTTCAGCAGC AGCAGCAGCA GCAGCAGCTG TTACAGAGCC AGAGGAAGTT ATCTCAAGCT 
GTGCGACAGC AGCAGGAGCA ACAGATTGCT CGTATGGTGA GTGCCCTCCA GCAGCAGCAG 
CAGAGGCAGC CTGGCATGAA GCATTCACCA TCCCACCCTG TTGGGCCTAA GCCACATATA 
GACAGCATGG TACCCAATCC TTTGAATGTG GGTCTTTCAG ATTTACAGAC CAAAGGACAA 
ATACCTGGAT ACGGTTCAGG CTTTGGCTCA AGCAGCATGG ATTATGGCAT GGTGGGAGGG 
AAAGAAGCTG GAACAGAATC CCGCTTTAAA CAGTGGACTA TGATGGAAGG GCTGCCCTCT 
GTGGCTTCAC AAGATGCCAA TATGCACAAA AATGGTGCAA TAGTGCCCCC TGGAAAGGCT 
CGCGGAGGAT CGCCCTACAA CCAGTTCGAC ATAATTCAGG GCGACCCACT AGGTGGCCAT 
GCAGGCCCTG CTGGTGATAG TTGGTTACCT GCCAAATCTC CACCGACAAA CAAGATCGGA 
AGCAAATCCA GCAACGCCAG CTGGCCTCCA GAGTTCCAAC CAGGAGTGCC ATGGAAAGGT 
ATACAAAACA TTGACCCTGA ATCTGACCCC TACGTGACCC CTGGAAGTGT GCTGGGGGGG 
ACAGCCACAT CTCCCATTGT AGATACTGAC CACCAACTTC TGCGGGACAA CACCACAGGG 
TCTAATTCTT CCCTCAACAC CTCGCTGCCT TCACCTGGTG CCTGGCCCTA CAGTGCCTCT 
GACAATTCCT TCACCAACGT TCATAGCACT TCAGCAAAAT TCAGTGATTA CAAATCAACA 
TGGTCCCCAG ATCCCATAGG ACACAATCCC ACTCATCTCT CCAACAAGAT GTGGAAAAAC 
CATATTTCCT CAAGGAACAC TACAGGGCTG CCTCGCCCGC CTCCTGGCCT GACCAACCCC 
AAACCATCAT CTCCATGGAA CAGCACAGCA CCCCGATCGG TCAGGGGCTG GGGGGCACAG 
GACTCAAGGC TTGCCTCTGG TGAGAAGGAT CATCTGATAA CGCCATTGAT CAGACCTGAG 
GAGTGTGCAT TTTACAGCAC TAATGGTTTG GAGGAGAGAA AGTTGCAGTT GCCTGCATCT 
ACTTGGAGTG ATGGTAGCTC AGTTCGTCCT AGCTACTGGC TGGTTCTTCA CAATCTCACT 
CCACAGATCG ATGGGTCAAC CTTGAGGACG ATCTGCATGC AGCATGGTCC ACTGCTGACA 
TTCCATCTGA ACCTGACCCA GGGCACCGCT CTCATCCGAT ACAACACCAA ACAGGAGGCG 
GCCAAGGCCC AGACTGCACT GCACATGTGT GTGTTGGGAA ACACTACCAT CCTTGCTGAG 
TTTGCCACAG ATGAAGAGGT TAGTCGCTTT TTGACACAAG CTCAGCCCCC TACACCTGCA 
GCAACCCCAA GTGCACCCAC GGCAGGCTGG CAATCCCTGG AGACAGGACA GAACCAGACA 
GATCCAGTTG GACCTGCTTT GAATCTCTTT GGTGGGTCAA CAGGGCTTGG GCAGTGGAGC 
AGTGGTGGCG GCGGCAGCAG TGGGGGTGAT CTCACTGGCG CTTCGTTGTG GGGGCCCCCA 
AACTATTCTT CAAGCTTGTG GGGGGTCCCA AGCGTAGAGG ATCCACACAG AATGGGCAGC 
CCTGCTCCCT TACTACCTGG AGACCTTCTG GGAGGAGGGT CGGATTCAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_015288813.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015144299.1 Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X8, mRNA.

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CloneID	OGa29596
Clone ID Related Accession (Same CDS sequence)	XM_015288813.1 , XM_015288813.2
Accession Version	XM_015288813.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	trinucleotide repeat-containing gene 6B protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288813.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACTG ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGGTCAG TGAGATGGAA 
CATGAGGATT TTGTAATGGA GGGGCATGGC AAGACTCCAC CTCCTGGTGA AGAAAACAAA 
CAAGAGAAGG AGCAAGAAAG GGAAGAACAG TTAATGGAAG ACAAGAAAAG GAAGAAGGAG 
GATAAGAAAA AGAAGGAATC TGCTCAGAAG GTCACAGATC AAAAAACCAA AGTGCCCGAA 
GTGACGAAAC CAAGTTTAAG CCAACCAGTG GCTGCCAGCC CAATTGGCAA CTCTCCATCG 
CCACCAGTCA ATGGTGGCAA CAATGCCAAA AGGGTGGCAG TGCCGAACGG ACAACCGCCA 
AGCGCCGCCC GCTACATGCC TCGGGAGGTG CCGCCGCGAT TCCGTTGCCA GCAGGACCAC 
AAAGTGTTAC TGAAACGTGG GCAGCCCCCT CCACCATCCT GTATGCTCCT TGGGGGTGGA 
GCAGGGCCTC CTCCCCCTAC AGCACCAGGA GCAAACCCAA ACAACGCACA ACCAGTGACA 
GGAGCACTGC TGCAGAGCGA CAGTGGGACT GCAACAGATT CAACAATTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGT CCTGGAGCCT CCTCCAGCAG CGGCACCAAC 
GCCAACCCAA CTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGTATCG CCAGCAAAGA TGCTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCGAACC CTGGCTTGGA GAAGAACACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGAAGCC AGTGCCAGTC TGGAAGCGCT GGAAATGAAT GTAATCTTGG GGCCTGGAAA 
TCTGATCCCA AGGCTAAATC TGTTCAATCT TCCAACCCTG CTACCGAGAG TAACAGTGGA 
CTAAGTAATT GGAGGAACTT GACTGGACAA GATAGAATTG GTTCTGGTTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCGTCTGCT TGGCCAGCAC TGGTCCAAGA GGGCAATTCT 
AGGAAAGGGA CTTCGGAATC TGATAGTGGT AGCTCCAATG CACAGATTAG CACAGTAGGT 
CAGACCTCTA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAACTT TGTCTCTGGC 
AGAGAACAGG CTCAAATTCA TAACACTGAT GGACCAAAAA ATGGAAACAC TAACTCCTTG 
AACTTAAGTT CACCAAACCC TATGGAGAAT AAGGGAATGC CCTTTGAAAT GGGCTTGGGG 
AACCCTTCCA GGAGCACTGA CACCCCTTCA CAAAGCACTG GAGAAAGAAA GACTGGGAGT 
GTTGGATCTT GGGGTACAGC TAGGGGGTCT TCTGGAACTG ACACAGTCTC TGGACAGAGC 
AATTCTGGAA ACCATGGGAA CAATGGAAAG GATAGGGAGG ACTCCTGGAA AGGAGCTTCT 
GTTCAAAAAC CTAATGGGTC AAGAAGCGAT TCTTGGGATA ACAATAACCG GTCTACGGGT 
GGTGGGTCTT GGAACTTTGG CCCTCAGCAT GCAAATGAAA GCAAATGGGG TGAAGGGAAC 
AAATTAACAT CTGGGGTCTC TCAGGGAGAA TGGAAACAGC TGTCTGGGTC TGATGAACTG 
AAGATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAAAAAGGCC ACCCTCTTCC TGAAAACCAA GGCAATTCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CTGCAGGAAG TGAGGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGACGC CGATCATACA GGCCTACGCA TCCTGATTGC 
CAGGCAGTCT TGCAGACTTT GCTGAGCAGG ACTGATTTGG ACCCCCGAGT GCTTTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAA GATACCGTAT GGGATATTGA AGAGATGCCA 
CGGTCCGAGG GGAAGTCTGA TAAAGGAACT GAGGGGTGGG AGAGCTCTGC CACACAGACA 
AAGAACTCAG GGGGCTGGGG CGATGTACCC AGCCAAAGCA ATCAAATCAA GTCTGGATGG 
GGTGAGCTCT CAACCCCAAC AGAATGGAAG GACCCCAAAA ATACTGGAGG ATGGAATGAC 
TATAAGAACC CCAATTCGTC TAATTGGGGA GGGGGAAGAC AAGAAGAGAA ATCGTCATCT 
TCTTGGAATG ACAGCTCTAG TAAGGATCAG GGGTGGGGTG GACGGCAGCC TAATCAAGGA 
TGGTCTTCTG GAAAGAATGG TTGGGGAGAG GAAGTTGACC AAGCAAAAAA CAGCAACTGG 
GAAAGTGCGG GAAATAAGCC AGCATCTGGT TGGGGTGAAG GTGGGCAAAA TGAGATTGGA 
ACTTGGGGTA ACGGTGCAAA TACAGGCTCT GCTTCAAAGG GTGGTTGGGA TGATTGTAAA 
AGAAATTCAA CGTGGAACGA GACGGGTCGA CAGCCCAACT CCTGGAACAA GCAGCAGCAG 
CAGCAACAGC AGCAACAGCA AGAGACTTCT GGTTCCTGGG GTCCACCGCC ACAAACTCCA 
AGTAATGGGC GACCTCCAAA CCCAAACTGG AACAGCGGAC CACAACCAGC TGCCCCCAAA 
GACGAGGAGC CTAGTGGCTG GGAAGAACCT TCTCCACAGT CAATCAGTCG AAAAATGGAT 
ATTGATGATG GCACTTCAGC GTGGGGAGAT CCTAGCAGTT ATAACTACAA GAATGTGAAC 
CTGTGGGACA AGAACTCACA AGGGGGACAG GCCCCACGGG AACAGAGCCT GCCTACTCCA 
ATGACTAGCA AATCACAAGC ATCAGTCTGG AGCAAAAGCA CTCCACCTGC TCCAGATAAT 
GGTACGTCCG CCTGGGGTGA GCCAAATGAA ACCAGTCCCG GGTGGGGTGA AGTAGATGAT 
ACAGGAGCAT CGACAACAGG CTGGGGGAAT GCACCTTCCA ACGCCCCGAA TGCCATGAAA 
TCTAGTTCTA AATCTATGCA AGATGGCTGG GGAGAGAGTG ATGGGCCAGT GACAGGAACA 
CGTCATTCCA GCTGGGAAGA GGAGGAGGAG GGAGGAGTCT GGAACACAGC AGGCTCTCAG 
GGAAGCAGTT CCTCCCACAA CTCAACAAGC TGGGGGCAAG GAGGAAAGAA AACGCAGATG 
AAGTGCTCGT TAAAAGGAGG AAATAGTGAT TCATGGATGA ACCCTATATC CAAGCAGTTT 
TCAAATATGG GCTTGCTAAG TCAAACTGAG GACATTCCAG GCAATAAGAT GGACTTGTCT 
GTAGGTGGTC TCCCAGATAA GAAGTTTGAG GTAGATAAAC GAGCCATGAA TCTCGGGGAT 
TTTAATGACA TAATGAGAAA GGATCGATCT GGGTTCCGTC CACCTAATTC CAAAGACATG 
GGAACCACAG ATAGTGGGCC TTATTTTGAG AAGGTTTCGG CCTCCATGCT CAAACAGTTC 
CCCAACAGTG GCTTGAATCC AGGTCTTTTC AATGTGGGGC CCCAGCTTTC TCCACAACAG 
ATTGCCATGC TGAGCCAGCT TCCACAGATT CCCCAGTTTC AATTAGCATG TCAGCTCCTC 
CTTCAGCAGC AGCAGCAGCA GCAGCAGCTG TTACAGAGCC AGAGGAAGTT ATCTCAAGCT 
GTGCGACAGC AGCAGGAGCA ACAGATTGCT CGTATGGTGA GTGCCCTCCA GCAGCAGCAG 
CAGAGGCAGC CTGGCATGAA GCATTCACCA TCCCACCCTG TTGGGCCTAA GCCACATATA 
GACAGCATGG TACCCAATCC TTTGAATGTG GGTCTTTCAG ATTTACAGAC CAAAGGACAA 
ATACCTGGAT ACGGTTCAGG CTTTGGCTCA AGCAGCATGG ATTATGGCAT GGTGGGAGGG 
AAAGAAGCTG GAACAGAATC CCGCTTTAAA CAGTGGACTA TGATGGAAGG GCTGCCCTCT 
GTGGCTTCAC AAGATGCCAA TATGCACAAA AATGGTGCAA TAGTGCCCCC TGGAAAGGCT 
CGCGGAGGAT CGCCCTACAA CCAGTTCGAC ATAATTCAGG GCGACCCACT AGGTGGCCAT 
GCAGGCCCTG CTGGTGATAG TTGGTTACCT GCCAAATCTC CACCGACAAA CAAGATCGGA 
AGCAAATCCA GCAACGCCAG CTGGCCTCCA GAGTTCCAAC CAGGAGTGCC ATGGAAAGGT 
ATACAAAACA TTGACCCTGA ATCTGACCCC TACGTGACCC CTGGAAGTGT GCTGGGGGGG 
ACAGCCACAT CTCCCATTGT AGATACTGAC CACCAACTTC TGCGGGACAA CACCACAGGG 
TCTAATTCTT CCCTCAACAC CTCGCTGCCT TCACCTGGTG CCTGGCCCTA CAGTGCCTCT 
GACAATTCCT TCACCAACGT TCATAGCACT TCAGCAAAAT TCAGTGATTA CAAATCAACA 
TGGTCCCCAG ATCCCATAGG ACACAATCCC ACTCATCTCT CCAACAAGAT GTGGAAAAAC 
CATATTTCCT CAAGGAACAC TACAGGGCTG CCTCGCCCGC CTCCTGGCCT GACCAACCCC 
AAACCATCAT CTCCATGGAA CAGCACAGCA CCCCGATCGG TCAGGGGCTG GGGGGCACAG 
GACTCAAGGC TTGCCTCTGG TGAGAAGGAT CATCTGATAA CGCCATTGAT CAGACCTGAG 
GAGTGTGCAT TTTACAGCAC TAATGGTTTG GAGGAGAGAA AGTTGCAGTT GCCTGCATCT 
ACTTGGAGTG ATGGTAGCTC AGTTCGTCCT AGCTACTGGC TGGTTCTTCA CAATCTCACT 
CCACAGATCG ATGGGTCAAC CTTGAGGACG ATCTGCATGC AGCATGGTCC ACTGCTGACA 
TTCCATCTGA ACCTGACCCA GGGCACCGCT CTCATCCGAT ACAACACCAA ACAGGAGGCG 
GCCAAGGCCC AGACTGCACT GCACATGTGT GTGTTGGGAA ACACTACCAT CCTTGCTGAG 
TTTGCCACAG ATGAAGAGGT TAGTCGCTTT TTGACACAAG CTCAGCCCCC TACACCTGCA 
GCAACCCCAA GTGCACCCAC GGCAGGCTGG CAATCCCTGG AGACAGGACA GAACCAGACA 
GATCCAGTTG GACCTGCTTT GAATCTCTTT GGTGGGTCAA CAGGGCTTGG GCAGTGGAGC 
AGTGGTGGCG GCGGCAGCAG TGGGGGTGAT CTCACTGGCG CTTCGTTGTG GGGGCCCCCA 
AACTATTCTT CAAGCTTGTG GGGGGTCCCA AGCGTAGAGG ATCCACACAG AATGGGCAGC 
CCTGCTCCCT TACTACCTGG AGACCTTCTG GGAGGAGGGT CGGATTCAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015144299.1
CDS	548..5941
Translation	MQTDEGEILEEGSPKVSEMEHEDFVMEGHGKTPPPGEENKQEKEQEREEQLMEDKKRKKEDKKKKESAQKVTDQKTKVPEVTKPSLSQPVAASPIGNSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPPTAPGANPNNAQPVTGALLQSDSGTATDSTIGGAAASNYANSTWGPGASSSSGTNANPTHIWDKVIVDGSDMEEWPCIASKDAESSSENTTDNNSASNPGLEKNTLPGSTTSNKGKGSQCQSGSAGNECNLGAWKSDPKAKSVQSSNPATESNSGLSNWRNLTGQDRIGSGSGFSNFNPNSNPSAWPALVQEGNSRKGTSESDSGSSNAQISTVGQTSREQQSKMENAGVNFVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFEMGLGNPSRSTDTPSQSTGERKTGSVGSWGTARGSSGTDTVSGQSNSGNHGNNGKDREDSWKGASVQKPNGSRSDSWDNNNRSTGGGSWNFGPQHANESKWGEGNKLTSGVSQGEWKQLSGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNSQAPCWGRSSSSAGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEMPRSEGKSDKGTEGWESSATQTKNSGGWGDVPSQSNQIKSGWGELSTPTEWKDPKNTGGWNDYKNPNSSNWGGGRQEEKSSSSWNDSSSKDQGWGGRQPNQGWSSGKNGWGEEVDQAKNSNWESAGNKPASGWGEGGQNEIGTWGNGANTGSASKGGWDDCKRNSTWNETGRQPNSWNKQQQQQQQQQQETSGSWGPPPQTPSNGRPPNPNWNSGPQPAAPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPSSYNYKNVNLWDKNSQGGQAPREQSLPTPMTSKSQASVWSKSTPPAPDNGTSAWGEPNETSPGWGEVDDTGASTTGWGNAPSNAPNAMKSSSKSMQDGWGESDGPVTGTRHSSWEEEEEGGVWNTAGSQGSSSSHNSTSWGQGGKKTQMKCSLKGGNSDSWMNPISKQFSNMGLLSQTEDIPGNKMDLSVGGLPDKKFEVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQQLLQSQRKLSQAVRQQQEQQIARMVSALQQQQQRQPGMKHSPSHPVGPKPHIDSMVPNPLNVGLSDLQTKGQIPGYGSGFGSSSMDYGMVGGKEAGTESRFKQWTMMEGLPSVASQDANMHKNGAIVPPGKARGGSPYNQFDIIQGDPLGGHAGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFSDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTGLPRPPPGLTNPKPSSPWNSTAPRSVRGWGAQDSRLASGEKDHLITPLIRPEECAFYSTNGLEERKLQLPASTWSDGSSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYNTKQEAAKAQTALHMCVLGNTTILAEFATDEEVSRFLTQAQPPTPAATPSAPTAGWQSLETGQNQTDPVGPALNLFGGSTGLGQWSSGGGGSSGGDLTGASLWGPPNYSSSLWGVPSVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_015288813.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015288813.2

ORF Insert Sequence:

ATGCAAACTG ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGGTCAG TGAGATGGAA 
CATGAGGATT TTGTAATGGA GGGGCATGGC AAGACTCCAC CTCCTGGTGA AGAAAACAAA 
CAAGAGAAGG AGCAAGAAAG GGAAGAACAG TTAATGGAAG ACAAGAAAAG GAAGAAGGAG 
GATAAGAAAA AGAAGGAATC TGCTCAGAAG GTCACAGATC AAAAAACCAA AGTGCCCGAA 
GTGACGAAAC CAAGTTTAAG CCAACCAGTG GCTGCCAGCC CAATTGGCAA CTCTCCATCG 
CCACCAGTCA ATGGTGGCAA CAATGCCAAA AGGGTGGCAG TGCCGAACGG ACAACCGCCA 
AGCGCCGCCC GCTACATGCC TCGGGAGGTG CCGCCGCGAT TCCGTTGCCA GCAGGACCAC 
AAAGTGTTAC TGAAACGTGG GCAGCCCCCT CCACCATCCT GTATGCTCCT TGGGGGTGGA 
GCAGGGCCTC CTCCCCCTAC AGCACCAGGA GCAAACCCAA ACAACGCACA ACCAGTGACA 
GGAGCACTGC TGCAGAGCGA CAGTGGGACT GCAACAGATT CAACAATTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGT CCTGGAGCCT CCTCCAGCAG CGGCACCAAC 
GCCAACCCAA CTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGTATCG CCAGCAAAGA TGCTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCGAACC CTGGCTTGGA GAAGAACACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGAAGCC AGTGCCAGTC TGGAAGCGCT GGAAATGAAT GTAATCTTGG GGCCTGGAAA 
TCTGATCCCA AGGCTAAATC TGTTCAATCT TCCAACCCTG CTACCGAGAG TAACAGTGGA 
CTAAGTAATT GGAGGAACTT GACTGGACAA GATAGAATTG GTTCTGGTTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCGTCTGCT TGGCCAGCAC TGGTCCAAGA GGGCAATTCT 
AGGAAAGGGA CTTCGGAATC TGATAGTGGT AGCTCCAATG CACAGATTAG CACAGTAGGT 
CAGACCTCTA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAACTT TGTCTCTGGC 
AGAGAACAGG CTCAAATTCA TAACACTGAT GGACCAAAAA ATGGAAACAC TAACTCCTTG 
AACTTAAGTT CACCAAACCC TATGGAGAAT AAGGGAATGC CCTTTGAAAT GGGCTTGGGG 
AACCCTTCCA GGAGCACTGA CACCCCTTCA CAAAGCACTG GAGAAAGAAA GACTGGGAGT 
GTTGGATCTT GGGGTACAGC TAGGGGGTCT TCTGGAACTG ACACAGTCTC TGGACAGAGC 
AATTCTGGAA ACCATGGGAA CAATGGAAAG GATAGGGAGG ACTCCTGGAA AGGAGCTTCT 
GTTCAAAAAC CTAATGGGTC AAGAAGCGAT TCTTGGGATA ACAATAACCG GTCTACGGGT 
GGTGGGTCTT GGAACTTTGG CCCTCAGCAT GCAAATGAAA GCAAATGGGG TGAAGGGAAC 
AAATTAACAT CTGGGGTCTC TCAGGGAGAA TGGAAACAGC TGTCTGGGTC TGATGAACTG 
AAGATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAAAAAGGCC ACCCTCTTCC TGAAAACCAA GGCAATTCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CTGCAGGAAG TGAGGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGACGC CGATCATACA GGCCTACGCA TCCTGATTGC 
CAGGCAGTCT TGCAGACTTT GCTGAGCAGG ACTGATTTGG ACCCCCGAGT GCTTTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAA GATACCGTAT GGGATATTGA AGAGATGCCA 
CGGTCCGAGG GGAAGTCTGA TAAAGGAACT GAGGGGTGGG AGAGCTCTGC CACACAGACA 
AAGAACTCAG GGGGCTGGGG CGATGTACCC AGCCAAAGCA ATCAAATCAA GTCTGGATGG 
GGTGAGCTCT CAACCCCAAC AGAATGGAAG GACCCCAAAA ATACTGGAGG ATGGAATGAC 
TATAAGAACC CCAATTCGTC TAATTGGGGA GGGGGAAGAC AAGAAGAGAA ATCGTCATCT 
TCTTGGAATG ACAGCTCTAG TAAGGATCAG GGGTGGGGTG GACGGCAGCC TAATCAAGGA 
TGGTCTTCTG GAAAGAATGG TTGGGGAGAG GAAGTTGACC AAGCAAAAAA CAGCAACTGG 
GAAAGTGCGG GAAATAAGCC AGCATCTGGT TGGGGTGAAG GTGGGCAAAA TGAGATTGGA 
ACTTGGGGTA ACGGTGCAAA TACAGGCTCT GCTTCAAAGG GTGGTTGGGA TGATTGTAAA 
AGAAATTCAA CGTGGAACGA GACGGGTCGA CAGCCCAACT CCTGGAACAA GCAGCAGCAG 
CAGCAACAGC AGCAACAGCA AGAGACTTCT GGTTCCTGGG GTCCACCGCC ACAAACTCCA 
AGTAATGGGC GACCTCCAAA CCCAAACTGG AACAGCGGAC CACAACCAGC TGCCCCCAAA 
GACGAGGAGC CTAGTGGCTG GGAAGAACCT TCTCCACAGT CAATCAGTCG AAAAATGGAT 
ATTGATGATG GCACTTCAGC GTGGGGAGAT CCTAGCAGTT ATAACTACAA GAATGTGAAC 
CTGTGGGACA AGAACTCACA AGGGGGACAG GCCCCACGGG AACAGAGCCT GCCTACTCCA 
ATGACTAGCA AATCACAAGC ATCAGTCTGG AGCAAAAGCA CTCCACCTGC TCCAGATAAT 
GGTACGTCCG CCTGGGGTGA GCCAAATGAA ACCAGTCCCG GGTGGGGTGA AGTAGATGAT 
ACAGGAGCAT CGACAACAGG CTGGGGGAAT GCACCTTCCA ACGCCCCGAA TGCCATGAAA 
TCTAGTTCTA AATCTATGCA AGATGGCTGG GGAGAGAGTG ATGGGCCAGT GACAGGAACA 
CGTCATTCCA GCTGGGAAGA GGAGGAGGAG GGAGGAGTCT GGAACACAGC AGGCTCTCAG 
GGAAGCAGTT CCTCCCACAA CTCAACAAGC TGGGGGCAAG GAGGAAAGAA AACGCAGATG 
AAGTGCTCGT TAAAAGGAGG AAATAGTGAT TCATGGATGA ACCCTATATC CAAGCAGTTT 
TCAAATATGG GCTTGCTAAG TCAAACTGAG GACATTCCAG GCAATAAGAT GGACTTGTCT 
GTAGGTGGTC TCCCAGATAA GAAGTTTGAG GTAGATAAAC GAGCCATGAA TCTCGGGGAT 
TTTAATGACA TAATGAGAAA GGATCGATCT GGGTTCCGTC CACCTAATTC CAAAGACATG 
GGAACCACAG ATAGTGGGCC TTATTTTGAG AAGGTTTCGG CCTCCATGCT CAAACAGTTC 
CCCAACAGTG GCTTGAATCC AGGTCTTTTC AATGTGGGGC CCCAGCTTTC TCCACAACAG 
ATTGCCATGC TGAGCCAGCT TCCACAGATT CCCCAGTTTC AATTAGCATG TCAGCTCCTC 
CTTCAGCAGC AGCAGCAGCA GCAGCAGCTG TTACAGAGCC AGAGGAAGTT ATCTCAAGCT 
GTGCGACAGC AGCAGGAGCA ACAGATTGCT CGTATGGTGA GTGCCCTCCA GCAGCAGCAG 
CAGAGGCAGC CTGGCATGAA GCATTCACCA TCCCACCCTG TTGGGCCTAA GCCACATATA 
GACAGCATGG TACCCAATCC TTTGAATGTG GGTCTTTCAG ATTTACAGAC CAAAGGACAA 
ATACCTGGAT ACGGTTCAGG CTTTGGCTCA AGCAGCATGG ATTATGGCAT GGTGGGAGGG 
AAAGAAGCTG GAACAGAATC CCGCTTTAAA CAGTGGACTA TGATGGAAGG GCTGCCCTCT 
GTGGCTTCAC AAGATGCCAA TATGCACAAA AATGGTGCAA TAGTGCCCCC TGGAAAGGCT 
CGCGGAGGAT CGCCCTACAA CCAGTTCGAC ATAATTCAGG GCGACCCACT AGGTGGCCAT 
GCAGGCCCTG CTGGTGATAG TTGGTTACCT GCCAAATCTC CACCGACAAA CAAGATCGGA 
AGCAAATCCA GCAACGCCAG CTGGCCTCCA GAGTTCCAAC CAGGAGTGCC ATGGAAAGGT 
ATACAAAACA TTGACCCTGA ATCTGACCCC TACGTGACCC CTGGAAGTGT GCTGGGGGGG 
ACAGCCACAT CTCCCATTGT AGATACTGAC CACCAACTTC TGCGGGACAA CACCACAGGG 
TCTAATTCTT CCCTCAACAC CTCGCTGCCT TCACCTGGTG CCTGGCCCTA CAGTGCCTCT 
GACAATTCCT TCACCAACGT TCATAGCACT TCAGCAAAAT TCAGTGATTA CAAATCAACA 
TGGTCCCCAG ATCCCATAGG ACACAATCCC ACTCATCTCT CCAACAAGAT GTGGAAAAAC 
CATATTTCCT CAAGGAACAC TACAGGGCTG CCTCGCCCGC CTCCTGGCCT GACCAACCCC 
AAACCATCAT CTCCATGGAA CAGCACAGCA CCCCGATCGG TCAGGGGCTG GGGGGCACAG 
GACTCAAGGC TTGCCTCTGG TGAGAAGGAT CATCTGATAA CGCCATTGAT CAGACCTGAG 
GAGTGTGCAT TTTACAGCAC TAATGGTTTG GAGGAGAGAA AGTTGCAGTT GCCTGCATCT 
ACTTGGAGTG ATGGTAGCTC AGTTCGTCCT AGCTACTGGC TGGTTCTTCA CAATCTCACT 
CCACAGATCG ATGGGTCAAC CTTGAGGACG ATCTGCATGC AGCATGGTCC ACTGCTGACA 
TTCCATCTGA ACCTGACCCA GGGCACCGCT CTCATCCGAT ACAACACCAA ACAGGAGGCG 
GCCAAGGCCC AGACTGCACT GCACATGTGT GTGTTGGGAA ACACTACCAT CCTTGCTGAG 
TTTGCCACAG ATGAAGAGGT TAGTCGCTTT TTGACACAAG CTCAGCCCCC TACACCTGCA 
GCAACCCCAA GTGCACCCAC GGCAGGCTGG CAATCCCTGG AGACAGGACA GAACCAGACA 
GATCCAGTTG GACCTGCTTT GAATCTCTTT GGTGGGTCAA CAGGGCTTGG GCAGTGGAGC 
AGTGGTGGCG GCGGCAGCAG TGGGGGTGAT CTCACTGGCG CTTCGTTGTG GGGGCCCCCA 
AACTATTCTT CAAGCTTGTG GGGGGTCCCA AGCGTAGAGG ATCCACACAG AATGGGCAGC 
CCTGCTCCCT TACTACCTGG AGACCTTCTG GGAGGAGGGT CGGATTCAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_004937731.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004937788.2 Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X9, mRNA.

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CloneID	OGa29597
Clone ID Related Accession (Same CDS sequence)	XM_004937731.2 , XM_004937731.3
Accession Version	XM_004937731.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	trinucleotide repeat-containing gene 6B protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455714.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004937731.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACTG ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGGTCAG TGAGATGGAA 
CATGAGGATT TTGTAATGGA GGGGCATGGC AAGACTCCAC CTCCTGGTGA AGAAAACAAA 
CAAGAGAAGG AGCAAGAAAG GGAAGAACAG TTAATGGAAG ACAAGAAAAG GAAGAAGGAG 
GATAAGAAAA AGAAGGAATC TGCTCAGAAG GTCACAGATC AAAAAACCAA AGTGCCCGAA 
GTGACGAAAC CAAGTTTAAG CCAACCAGTG GCTGCCAGCC CAATTGGCAA CTCTCCATCG 
CCACCAGTCA ATGGTGGCAA CAATGCCAAA AGGGTGGCAG TGCCGAACGG ACAACCGCCA 
AGCGCCGCCC GCTACATGCC TCGGGAGGTG CCGCCGCGAT TCCGTTGCCA GCAGGACCAC 
AAAGTGTTAC TGAAACGTGG GCAGCCCCCT CCACCATCCT GTATGCTCCT TGGGGGTGGA 
GCAGGGCCTC CTCCCCCTAC AGCACCAGGA GCAAACCCAA ACAACGCACA ACCAGTGACA 
GGAGCACTGC TGCAGAGCGA CAGTGGGACT GCAACAGATT CAACAATTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGT CCTGGAGCCT CCTCCAGCAG CGGCACCAAC 
GCCAACCCAA CTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGTATCG CCAGCAAAGA TGCTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCGAACC CTGGCTTGGA GAAGAACACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGAAGCC AGTGCCAGTC TGGAAGCGCT GGAAATGAAT GTAATCTTGG GGCCTGGAAA 
TCTGATCCCA AGGCTAAATC TGTTCAATCT TCCAACCCTG CTACCGAGAG TAACAGTGGA 
CTAAGTAATT GGAGGAACTT GACTGGACAA GATAGAATTG GTTCTGGTTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCGTCTGCT TGGCCAGCAC TGGTCCAAGA GGGCAATTCT 
AGGAAAGGGA CTTCGGAATC TGATAGTGGT AGCTCCAATG CACAGATTAG CACAGTAGGT 
CAGACCTCTA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAACTT TGTCTCTGGC 
AGAGAACAGG CTCAAATTCA TAACACTGAT GGACCAAAAA ATGGAAACAC TAACTCCTTG 
AACTTAAGTT CACCAAACCC TATGGAGAAT AAGGGAATGC CCTTTGAAAT GGGCTTGGGG 
AACCCTTCCA GGAGCACTGA CACCCCTTCA CAAAGCACTG GAGAAAGAAA GACTGGGAGT 
GTTGGATCTT GGGGTACAGC TAGGGGGTCT TCTGGAACTG ACACAGTCTC TGGACAGAGC 
AATTCTGGAA ACCATGGGAA CAATGGAAAG GATAGGGAGG ACTCCTGGAA AGGAGCTTCT 
GTTCAAAAAC CTAATGGGTC AAGAAGCGAT TCTTGGGATA ACAATAACCG GTCTACGGGT 
GGTGGGTCTT GGAACTTTGG CCCTCAGCAT GCAAATGAAA GCAAATGGGG TGAAGGGAAC 
AAATTAACAT CTGGGGTCTC TCAGGGAGAA TGGAAACAGC TGTCTGGGTC TGATGAACTG 
AAGATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAAAAAGGCC ACCCTCTTCC TGAAAACCAA GGCAATTCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CTGCAGGAAG TGAGGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGACGC CGATCATACA GGCCTACGCA TCCTGATTGC 
CAGGCAGTCT TGCAGACTTT GCTGAGCAGG ACTGATTTGG ACCCCCGAGT GCTTTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAA GATACCGTAT GGGATATTGA AGAGATGCCA 
CGGTCCGAGG GGAAGTCTGA TAAAGGAACT GAGGGGTGGG AGAGCTCTGC CACACAGACA 
AAGAACTCAG GGGGCTGGGG CGATGTACCC AGCCAAAGCA ATCAAATCAA GTCTGGATGG 
GGTGAGCTCT CAACCCCAAC AGAATGGAAG GACCCCAAAA ATACTGGAGG ATGGAATGAC 
TATAAGAACC CCAATTCGTC TAATTGGGGA GGGGGAAGAC AAGAAGAGAA ATCGTCATCT 
TCTTGGAATG ACAGCTCTAG TAAGGATCAG GGGTGGGGTG GACGGCAGCC TAATCAAGGA 
TGGTCTTCTG GAAAGAATGG TTGGGGAGAG GAAGTTGACC AAGCAAAAAA CAGCAACTGG 
GAAAGTGCGG GAAATAAGCC AGCATCTGGT TGGGGTGAAG GTGGGCAAAA TGAGATTGGA 
ACTTGGGGTA ACGGTGCAAA TACAGGCTCT GCTTCAAAGG GTGGTTGGGA TGATTGTAAA 
AGAAATTCAA CGTGGAACGA GACGGGTCGA CAGCCCAACT CCTGGAACAA GCAGCAGCAG 
CAGCAACAGC AGCAACAGCA AGAGACTTCT GGTTCCTGGG GTCCACCGCC ACAAACTCCA 
AGTAATGGGC GACCTCCAAA CCCAAACTGG AACAGCGGAC CACAACCAGC TGCCCCCAAA 
GACGAGGAGC CTAGTGGCTG GGAAGAACCT TCTCCACAGT CAATCAGTCG AAAAATGGAT 
ATTGATGATG GCACTTCAGC GTGGGGAGAT CCTAGCAGTT ATAACTACAA GAATGTGAAC 
CTGTGGGACA AGAACTCACA AGGGGGACAG GCCCCACGGG AACAGAGCCT GCCTACTCCA 
ATGACTAGCA AATCACAAGC ATCAGGTTCT AAATCTATGC AAGATGGCTG GGGAGAGAGT 
GATGGGCCAG TGACAGGAAC ACGTCATTCC AGCTGGGAAG AGGAGGAGGA GGGAGGAGTC 
TGGAACACAG CAGGCTCTCA GGGAAGCAGT TCCTCCCACA ACTCAACAAG CTGGGGGCAA 
GGAGGAAAGA AAACGCAGAT GAAGTGCTCG TTAAAAGGAG GAAATAGTGA TTCATGGATG 
AACCCTATAT CCAAGCAGTT TTCAAATATG GGCTTGCTAA GTCAAACTGA GGACATTCCA 
GGCAATAAGA TGGACTTGTC TGTAGGTGGT CTCCCAGATA AGAAGTTTGA GGTAGATAAA 
CGAGCCATGA ATCTCGGGGA TTTTAATGAC ATAATGAGAA AGGATCGATC TGGGTTCCGT 
CCACCTAATT CCAAAGACAT GGGAACCACA GATAGTGGGC CTTATTTTGA GAAGGGTGGC 
AATCATGGTT TGTTTGGAAA CAGCACAGCA CAATCGAGGG GCATGCACAC ACCAGTGCAG 
CCACTAAATC CTTCCCCCAA TATCCGGGCG CAAGTGCCTC CCCAATTTCT TTCTCCCCAG 
GTTTCGGCCT CCATGCTCAA ACAGTTCCCC AACAGTGGCT TGAATCCAGG TCTTTTCAAT 
GTGGGGCCCC AGCTTTCTCC ACAACAGATT GCCATGCTGA GCCAGCTTCC ACAGATTCCC 
CAGTTTCAAT TAGCATGTCA GCTCCTCCTT CAGCAGCAGC AGCAGCAGCA GCAGCTGTTA 
CAGAGCCAGA GGAAGTTATC TCAAGCTGTG CGACAGCAGC AGGAGCAACA GATTGCTCGT 
ATGGTGAGTG CCCTCCAGCA GCAGCAGCAG AGGCAGCCTG GCATGAAGCA TTCACCATCC 
CACCCTGTTG GGCCTAAGCC ACATATAGAC AGCATGGTAC CCAATCCTTT GAATGTGGGT 
CTTTCAGATT TACAGACCAA AGGACAAATA CCTGGATACG GTTCAGGCTT TGGCTCAAGC 
AGCATGGATT ATGGCATGGT GGGAGGGAAA GAAGCTGGAA CAGAATCCCG CTTTAAACAG 
TGGACTATGA TGGAAGGGCT GCCCTCTGTG GCTTCACAAG ATGCCAATAT GCACAAAAAT 
GGTGCAATAG TGCCCCCTGG AAAGGCTCGC GGAGGATCGC CCTACAACCA GTTCGACATA 
ATTCAGGGCG ACCCACTAGG TGGCCATGCA GGCCCTGCTG GTGATAGTTG GTTACCTGCC 
AAATCTCCAC CGACAAACAA GATCGGAAGC AAATCCAGCA ACGCCAGCTG GCCTCCAGAG 
TTCCAACCAG GAGTGCCATG GAAAGGTATA CAAAACATTG ACCCTGAATC TGACCCCTAC 
GTGACCCCTG GAAGTGTGCT GGGGGGGACA GCCACATCTC CCATTGTAGA TACTGACCAC 
CAACTTCTGC GGGACAACAC CACAGGGTCT AATTCTTCCC TCAACACCTC GCTGCCTTCA 
CCTGGTGCCT GGCCCTACAG TGCCTCTGAC AATTCCTTCA CCAACGTTCA TAGCACTTCA 
GCAAAATTCA GTGATTACAA ATCAACATGG TCCCCAGATC CCATAGGACA CAATCCCACT 
CATCTCTCCA ACAAGATGTG GAAAAACCAT ATTTCCTCAA GGAACACTAC AGGGCTGCCT 
CGCCCGCCTC CTGGCCTGAC CAACCCCAAA CCATCATCTC CATGGAACAG CACAGCACCC 
CGATCGGTCA GGGGCTGGGG GGCACAGGAC TCAAGGCTTG CCTCTGCATC TACTTGGAGT 
GATGGTAGCT CAGTTCGTCC TAGCTACTGG CTGGTTCTTC ACAATCTCAC TCCACAGATC 
GATGGGTCAA CCTTGAGGAC GATCTGCATG CAGCATGGTC CACTGCTGAC ATTCCATCTG 
AACCTGACCC AGGGCACCGC TCTCATCCGA TACAACACCA AACAGGAGGC GGCCAAGGCC 
CAGACTGCAC TGCACATGTG TGTGTTGGGA AACACTACCA TCCTTGCTGA GTTTGCCACA 
GATGAAGAGG TTAGTCGCTT TTTGACACAA GCTCAGCCCC CTACACCTGC AGCAACCCCA 
AGTGCACCCA CGGCAGGCTG GCAATCCCTG GAGACAGGAC AGAACCAGAC AGATCCAGTT 
GGACCTGCTT TGAATCTCTT TGGTGGGTCA ACAGGGCTTG GGCAGTGGAG CAGTGGTGGC 
GGCGGCAGCA GTGGGGGTGA TCTCACTGGC GCTTCGTTGT GGGGGCCCCC AAACTATTCT 
TCAAGCTTGT GGGGGGTCCC AAGCGTAGAG GATCCACACA GAATGGGCAG CCCTGCTCCC 
TTACTACCTG GAGACCTTCT GGGAGGAGGG TCGGATTCAA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004937788.2
CDS	535..5799
Translation	MQTDEGEILEEGSPKVSEMEHEDFVMEGHGKTPPPGEENKQEKEQEREEQLMEDKKRKKEDKKKKESAQKVTDQKTKVPEVTKPSLSQPVAASPIGNSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPPTAPGANPNNAQPVTGALLQSDSGTATDSTIGGAAASNYANSTWGPGASSSSGTNANPTHIWDKVIVDGSDMEEWPCIASKDAESSSENTTDNNSASNPGLEKNTLPGSTTSNKGKGSQCQSGSAGNECNLGAWKSDPKAKSVQSSNPATESNSGLSNWRNLTGQDRIGSGSGFSNFNPNSNPSAWPALVQEGNSRKGTSESDSGSSNAQISTVGQTSREQQSKMENAGVNFVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFEMGLGNPSRSTDTPSQSTGERKTGSVGSWGTARGSSGTDTVSGQSNSGNHGNNGKDREDSWKGASVQKPNGSRSDSWDNNNRSTGGGSWNFGPQHANESKWGEGNKLTSGVSQGEWKQLSGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNSQAPCWGRSSSSAGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEMPRSEGKSDKGTEGWESSATQTKNSGGWGDVPSQSNQIKSGWGELSTPTEWKDPKNTGGWNDYKNPNSSNWGGGRQEEKSSSSWNDSSSKDQGWGGRQPNQGWSSGKNGWGEEVDQAKNSNWESAGNKPASGWGEGGQNEIGTWGNGANTGSASKGGWDDCKRNSTWNETGRQPNSWNKQQQQQQQQQQETSGSWGPPPQTPSNGRPPNPNWNSGPQPAAPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPSSYNYKNVNLWDKNSQGGQAPREQSLPTPMTSKSQASGSKSMQDGWGESDGPVTGTRHSSWEEEEEGGVWNTAGSQGSSSSHNSTSWGQGGKKTQMKCSLKGGNSDSWMNPISKQFSNMGLLSQTEDIPGNKMDLSVGGLPDKKFEVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGMHTPVQPLNPSPNIRAQVPPQFLSPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQQLLQSQRKLSQAVRQQQEQQIARMVSALQQQQQRQPGMKHSPSHPVGPKPHIDSMVPNPLNVGLSDLQTKGQIPGYGSGFGSSSMDYGMVGGKEAGTESRFKQWTMMEGLPSVASQDANMHKNGAIVPPGKARGGSPYNQFDIIQGDPLGGHAGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFSDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTGLPRPPPGLTNPKPSSPWNSTAPRSVRGWGAQDSRLASASTWSDGSSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYNTKQEAAKAQTALHMCVLGNTTILAEFATDEEVSRFLTQAQPPTPAATPSAPTAGWQSLETGQNQTDPVGPALNLFGGSTGLGQWSSGGGGSSGGDLTGASLWGPPNYSSSLWGVPSVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_004937731.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004937731.2

ORF Insert Sequence:

ATGCAAACTG ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGGTCAG TGAGATGGAA 
CATGAGGATT TTGTAATGGA GGGGCATGGC AAGACTCCAC CTCCTGGTGA AGAAAACAAA 
CAAGAGAAGG AGCAAGAAAG GGAAGAACAG TTAATGGAAG ACAAGAAAAG GAAGAAGGAG 
GATAAGAAAA AGAAGGAATC TGCTCAGAAG GTCACAGATC AAAAAACCAA AGTGCCCGAA 
GTGACGAAAC CAAGTTTAAG CCAACCAGTG GCTGCCAGCC CAATTGGCAA CTCTCCATCG 
CCACCAGTCA ATGGTGGCAA CAATGCCAAA AGGGTGGCAG TGCCGAACGG ACAACCGCCA 
AGCGCCGCCC GCTACATGCC TCGGGAGGTG CCGCCGCGAT TCCGTTGCCA GCAGGACCAC 
AAAGTGTTAC TGAAACGTGG GCAGCCCCCT CCACCATCCT GTATGCTCCT TGGGGGTGGA 
GCAGGGCCTC CTCCCCCTAC AGCACCAGGA GCAAACCCAA ACAACGCACA ACCAGTGACA 
GGAGCACTGC TGCAGAGCGA CAGTGGGACT GCAACAGATT CAACAATTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGT CCTGGAGCCT CCTCCAGCAG CGGCACCAAC 
GCCAACCCAA CTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGTATCG CCAGCAAAGA TGCTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCGAACC CTGGCTTGGA GAAGAACACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGAAGCC AGTGCCAGTC TGGAAGCGCT GGAAATGAAT GTAATCTTGG GGCCTGGAAA 
TCTGATCCCA AGGCTAAATC TGTTCAATCT TCCAACCCTG CTACCGAGAG TAACAGTGGA 
CTAAGTAATT GGAGGAACTT GACTGGACAA GATAGAATTG GTTCTGGTTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCGTCTGCT TGGCCAGCAC TGGTCCAAGA GGGCAATTCT 
AGGAAAGGGA CTTCGGAATC TGATAGTGGT AGCTCCAATG CACAGATTAG CACAGTAGGT 
CAGACCTCTA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAACTT TGTCTCTGGC 
AGAGAACAGG CTCAAATTCA TAACACTGAT GGACCAAAAA ATGGAAACAC TAACTCCTTG 
AACTTAAGTT CACCAAACCC TATGGAGAAT AAGGGAATGC CCTTTGAAAT GGGCTTGGGG 
AACCCTTCCA GGAGCACTGA CACCCCTTCA CAAAGCACTG GAGAAAGAAA GACTGGGAGT 
GTTGGATCTT GGGGTACAGC TAGGGGGTCT TCTGGAACTG ACACAGTCTC TGGACAGAGC 
AATTCTGGAA ACCATGGGAA CAATGGAAAG GATAGGGAGG ACTCCTGGAA AGGAGCTTCT 
GTTCAAAAAC CTAATGGGTC AAGAAGCGAT TCTTGGGATA ACAATAACCG GTCTACGGGT 
GGTGGGTCTT GGAACTTTGG CCCTCAGCAT GCAAATGAAA GCAAATGGGG TGAAGGGAAC 
AAATTAACAT CTGGGGTCTC TCAGGGAGAA TGGAAACAGC TGTCTGGGTC TGATGAACTG 
AAGATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAAAAAGGCC ACCCTCTTCC TGAAAACCAA GGCAATTCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CTGCAGGAAG TGAGGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGACGC CGATCATACA GGCCTACGCA TCCTGATTGC 
CAGGCAGTCT TGCAGACTTT GCTGAGCAGG ACTGATTTGG ACCCCCGAGT GCTTTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAA GATACCGTAT GGGATATTGA AGAGATGCCA 
CGGTCCGAGG GGAAGTCTGA TAAAGGAACT GAGGGGTGGG AGAGCTCTGC CACACAGACA 
AAGAACTCAG GGGGCTGGGG CGATGTACCC AGCCAAAGCA ATCAAATCAA GTCTGGATGG 
GGTGAGCTCT CAACCCCAAC AGAATGGAAG GACCCCAAAA ATACTGGAGG ATGGAATGAC 
TATAAGAACC CCAATTCGTC TAATTGGGGA GGGGGAAGAC AAGAAGAGAA ATCGTCATCT 
TCTTGGAATG ACAGCTCTAG TAAGGATCAG GGGTGGGGTG GACGGCAGCC TAATCAAGGA 
TGGTCTTCTG GAAAGAATGG TTGGGGAGAG GAAGTTGACC AAGCAAAAAA CAGCAACTGG 
GAAAGTGCGG GAAATAAGCC AGCATCTGGT TGGGGTGAAG GTGGGCAAAA TGAGATTGGA 
ACTTGGGGTA ACGGTGCAAA TACAGGCTCT GCTTCAAAGG GTGGTTGGGA TGATTGTAAA 
AGAAATTCAA CGTGGAACGA GACGGGTCGA CAGCCCAACT CCTGGAACAA GCAGCAGCAG 
CAGCAACAGC AGCAACAGCA AGAGACTTCT GGTTCCTGGG GTCCACCGCC ACAAACTCCA 
AGTAATGGGC GACCTCCAAA CCCAAACTGG AACAGCGGAC CACAACCAGC TGCCCCCAAA 
GACGAGGAGC CTAGTGGCTG GGAAGAACCT TCTCCACAGT CAATCAGTCG AAAAATGGAT 
ATTGATGATG GCACTTCAGC GTGGGGAGAT CCTAGCAGTT ATAACTACAA GAATGTGAAC 
CTGTGGGACA AGAACTCACA AGGGGGACAG GCCCCACGGG AACAGAGCCT GCCTACTCCA 
ATGACTAGCA AATCACAAGC ATCAGGTTCT AAATCTATGC AAGATGGCTG GGGAGAGAGT 
GATGGGCCAG TGACAGGAAC ACGTCATTCC AGCTGGGAAG AGGAGGAGGA GGGAGGAGTC 
TGGAACACAG CAGGCTCTCA GGGAAGCAGT TCCTCCCACA ACTCAACAAG CTGGGGGCAA 
GGAGGAAAGA AAACGCAGAT GAAGTGCTCG TTAAAAGGAG GAAATAGTGA TTCATGGATG 
AACCCTATAT CCAAGCAGTT TTCAAATATG GGCTTGCTAA GTCAAACTGA GGACATTCCA 
GGCAATAAGA TGGACTTGTC TGTAGGTGGT CTCCCAGATA AGAAGTTTGA GGTAGATAAA 
CGAGCCATGA ATCTCGGGGA TTTTAATGAC ATAATGAGAA AGGATCGATC TGGGTTCCGT 
CCACCTAATT CCAAAGACAT GGGAACCACA GATAGTGGGC CTTATTTTGA GAAGGGTGGC 
AATCATGGTT TGTTTGGAAA CAGCACAGCA CAATCGAGGG GCATGCACAC ACCAGTGCAG 
CCACTAAATC CTTCCCCCAA TATCCGGGCG CAAGTGCCTC CCCAATTTCT TTCTCCCCAG 
GTTTCGGCCT CCATGCTCAA ACAGTTCCCC AACAGTGGCT TGAATCCAGG TCTTTTCAAT 
GTGGGGCCCC AGCTTTCTCC ACAACAGATT GCCATGCTGA GCCAGCTTCC ACAGATTCCC 
CAGTTTCAAT TAGCATGTCA GCTCCTCCTT CAGCAGCAGC AGCAGCAGCA GCAGCTGTTA 
CAGAGCCAGA GGAAGTTATC TCAAGCTGTG CGACAGCAGC AGGAGCAACA GATTGCTCGT 
ATGGTGAGTG CCCTCCAGCA GCAGCAGCAG AGGCAGCCTG GCATGAAGCA TTCACCATCC 
CACCCTGTTG GGCCTAAGCC ACATATAGAC AGCATGGTAC CCAATCCTTT GAATGTGGGT 
CTTTCAGATT TACAGACCAA AGGACAAATA CCTGGATACG GTTCAGGCTT TGGCTCAAGC 
AGCATGGATT ATGGCATGGT GGGAGGGAAA GAAGCTGGAA CAGAATCCCG CTTTAAACAG 
TGGACTATGA TGGAAGGGCT GCCCTCTGTG GCTTCACAAG ATGCCAATAT GCACAAAAAT 
GGTGCAATAG TGCCCCCTGG AAAGGCTCGC GGAGGATCGC CCTACAACCA GTTCGACATA 
ATTCAGGGCG ACCCACTAGG TGGCCATGCA GGCCCTGCTG GTGATAGTTG GTTACCTGCC 
AAATCTCCAC CGACAAACAA GATCGGAAGC AAATCCAGCA ACGCCAGCTG GCCTCCAGAG 
TTCCAACCAG GAGTGCCATG GAAAGGTATA CAAAACATTG ACCCTGAATC TGACCCCTAC 
GTGACCCCTG GAAGTGTGCT GGGGGGGACA GCCACATCTC CCATTGTAGA TACTGACCAC 
CAACTTCTGC GGGACAACAC CACAGGGTCT AATTCTTCCC TCAACACCTC GCTGCCTTCA 
CCTGGTGCCT GGCCCTACAG TGCCTCTGAC AATTCCTTCA CCAACGTTCA TAGCACTTCA 
GCAAAATTCA GTGATTACAA ATCAACATGG TCCCCAGATC CCATAGGACA CAATCCCACT 
CATCTCTCCA ACAAGATGTG GAAAAACCAT ATTTCCTCAA GGAACACTAC AGGGCTGCCT 
CGCCCGCCTC CTGGCCTGAC CAACCCCAAA CCATCATCTC CATGGAACAG CACAGCACCC 
CGATCGGTCA GGGGCTGGGG GGCACAGGAC TCAAGGCTTG CCTCTGCATC TACTTGGAGT 
GATGGTAGCT CAGTTCGTCC TAGCTACTGG CTGGTTCTTC ACAATCTCAC TCCACAGATC 
GATGGGTCAA CCTTGAGGAC GATCTGCATG CAGCATGGTC CACTGCTGAC ATTCCATCTG 
AACCTGACCC AGGGCACCGC TCTCATCCGA TACAACACCA AACAGGAGGC GGCCAAGGCC 
CAGACTGCAC TGCACATGTG TGTGTTGGGA AACACTACCA TCCTTGCTGA GTTTGCCACA 
GATGAAGAGG TTAGTCGCTT TTTGACACAA GCTCAGCCCC CTACACCTGC AGCAACCCCA 
AGTGCACCCA CGGCAGGCTG GCAATCCCTG GAGACAGGAC AGAACCAGAC AGATCCAGTT 
GGACCTGCTT TGAATCTCTT TGGTGGGTCA ACAGGGCTTG GGCAGTGGAG CAGTGGTGGC 
GGCGGCAGCA GTGGGGGTGA TCTCACTGGC GCTTCGTTGT GGGGGCCCCC AAACTATTCT 
TCAAGCTTGT GGGGGGTCCC AAGCGTAGAG GATCCACACA GAATGGGCAG CCCTGCTCCC 
TTACTACCTG GAGACCTTCT GGGAGGAGGG TCGGATTCAA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_004937731.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004937788.2 Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X9, mRNA.

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CloneID	OGa29597
Clone ID Related Accession (Same CDS sequence)	XM_004937731.2 , XM_004937731.3
Accession Version	XM_004937731.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	trinucleotide repeat-containing gene 6B protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004937731.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACTG ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGGTCAG TGAGATGGAA 
CATGAGGATT TTGTAATGGA GGGGCATGGC AAGACTCCAC CTCCTGGTGA AGAAAACAAA 
CAAGAGAAGG AGCAAGAAAG GGAAGAACAG TTAATGGAAG ACAAGAAAAG GAAGAAGGAG 
GATAAGAAAA AGAAGGAATC TGCTCAGAAG GTCACAGATC AAAAAACCAA AGTGCCCGAA 
GTGACGAAAC CAAGTTTAAG CCAACCAGTG GCTGCCAGCC CAATTGGCAA CTCTCCATCG 
CCACCAGTCA ATGGTGGCAA CAATGCCAAA AGGGTGGCAG TGCCGAACGG ACAACCGCCA 
AGCGCCGCCC GCTACATGCC TCGGGAGGTG CCGCCGCGAT TCCGTTGCCA GCAGGACCAC 
AAAGTGTTAC TGAAACGTGG GCAGCCCCCT CCACCATCCT GTATGCTCCT TGGGGGTGGA 
GCAGGGCCTC CTCCCCCTAC AGCACCAGGA GCAAACCCAA ACAACGCACA ACCAGTGACA 
GGAGCACTGC TGCAGAGCGA CAGTGGGACT GCAACAGATT CAACAATTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGT CCTGGAGCCT CCTCCAGCAG CGGCACCAAC 
GCCAACCCAA CTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGTATCG CCAGCAAAGA TGCTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCGAACC CTGGCTTGGA GAAGAACACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGAAGCC AGTGCCAGTC TGGAAGCGCT GGAAATGAAT GTAATCTTGG GGCCTGGAAA 
TCTGATCCCA AGGCTAAATC TGTTCAATCT TCCAACCCTG CTACCGAGAG TAACAGTGGA 
CTAAGTAATT GGAGGAACTT GACTGGACAA GATAGAATTG GTTCTGGTTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCGTCTGCT TGGCCAGCAC TGGTCCAAGA GGGCAATTCT 
AGGAAAGGGA CTTCGGAATC TGATAGTGGT AGCTCCAATG CACAGATTAG CACAGTAGGT 
CAGACCTCTA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAACTT TGTCTCTGGC 
AGAGAACAGG CTCAAATTCA TAACACTGAT GGACCAAAAA ATGGAAACAC TAACTCCTTG 
AACTTAAGTT CACCAAACCC TATGGAGAAT AAGGGAATGC CCTTTGAAAT GGGCTTGGGG 
AACCCTTCCA GGAGCACTGA CACCCCTTCA CAAAGCACTG GAGAAAGAAA GACTGGGAGT 
GTTGGATCTT GGGGTACAGC TAGGGGGTCT TCTGGAACTG ACACAGTCTC TGGACAGAGC 
AATTCTGGAA ACCATGGGAA CAATGGAAAG GATAGGGAGG ACTCCTGGAA AGGAGCTTCT 
GTTCAAAAAC CTAATGGGTC AAGAAGCGAT TCTTGGGATA ACAATAACCG GTCTACGGGT 
GGTGGGTCTT GGAACTTTGG CCCTCAGCAT GCAAATGAAA GCAAATGGGG TGAAGGGAAC 
AAATTAACAT CTGGGGTCTC TCAGGGAGAA TGGAAACAGC TGTCTGGGTC TGATGAACTG 
AAGATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAAAAAGGCC ACCCTCTTCC TGAAAACCAA GGCAATTCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CTGCAGGAAG TGAGGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGACGC CGATCATACA GGCCTACGCA TCCTGATTGC 
CAGGCAGTCT TGCAGACTTT GCTGAGCAGG ACTGATTTGG ACCCCCGAGT GCTTTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAA GATACCGTAT GGGATATTGA AGAGATGCCA 
CGGTCCGAGG GGAAGTCTGA TAAAGGAACT GAGGGGTGGG AGAGCTCTGC CACACAGACA 
AAGAACTCAG GGGGCTGGGG CGATGTACCC AGCCAAAGCA ATCAAATCAA GTCTGGATGG 
GGTGAGCTCT CAACCCCAAC AGAATGGAAG GACCCCAAAA ATACTGGAGG ATGGAATGAC 
TATAAGAACC CCAATTCGTC TAATTGGGGA GGGGGAAGAC AAGAAGAGAA ATCGTCATCT 
TCTTGGAATG ACAGCTCTAG TAAGGATCAG GGGTGGGGTG GACGGCAGCC TAATCAAGGA 
TGGTCTTCTG GAAAGAATGG TTGGGGAGAG GAAGTTGACC AAGCAAAAAA CAGCAACTGG 
GAAAGTGCGG GAAATAAGCC AGCATCTGGT TGGGGTGAAG GTGGGCAAAA TGAGATTGGA 
ACTTGGGGTA ACGGTGCAAA TACAGGCTCT GCTTCAAAGG GTGGTTGGGA TGATTGTAAA 
AGAAATTCAA CGTGGAACGA GACGGGTCGA CAGCCCAACT CCTGGAACAA GCAGCAGCAG 
CAGCAACAGC AGCAACAGCA AGAGACTTCT GGTTCCTGGG GTCCACCGCC ACAAACTCCA 
AGTAATGGGC GACCTCCAAA CCCAAACTGG AACAGCGGAC CACAACCAGC TGCCCCCAAA 
GACGAGGAGC CTAGTGGCTG GGAAGAACCT TCTCCACAGT CAATCAGTCG AAAAATGGAT 
ATTGATGATG GCACTTCAGC GTGGGGAGAT CCTAGCAGTT ATAACTACAA GAATGTGAAC 
CTGTGGGACA AGAACTCACA AGGGGGACAG GCCCCACGGG AACAGAGCCT GCCTACTCCA 
ATGACTAGCA AATCACAAGC ATCAGGTTCT AAATCTATGC AAGATGGCTG GGGAGAGAGT 
GATGGGCCAG TGACAGGAAC ACGTCATTCC AGCTGGGAAG AGGAGGAGGA GGGAGGAGTC 
TGGAACACAG CAGGCTCTCA GGGAAGCAGT TCCTCCCACA ACTCAACAAG CTGGGGGCAA 
GGAGGAAAGA AAACGCAGAT GAAGTGCTCG TTAAAAGGAG GAAATAGTGA TTCATGGATG 
AACCCTATAT CCAAGCAGTT TTCAAATATG GGCTTGCTAA GTCAAACTGA GGACATTCCA 
GGCAATAAGA TGGACTTGTC TGTAGGTGGT CTCCCAGATA AGAAGTTTGA GGTAGATAAA 
CGAGCCATGA ATCTCGGGGA TTTTAATGAC ATAATGAGAA AGGATCGATC TGGGTTCCGT 
CCACCTAATT CCAAAGACAT GGGAACCACA GATAGTGGGC CTTATTTTGA GAAGGGTGGC 
AATCATGGTT TGTTTGGAAA CAGCACAGCA CAATCGAGGG GCATGCACAC ACCAGTGCAG 
CCACTAAATC CTTCCCCCAA TATCCGGGCG CAAGTGCCTC CCCAATTTCT TTCTCCCCAG 
GTTTCGGCCT CCATGCTCAA ACAGTTCCCC AACAGTGGCT TGAATCCAGG TCTTTTCAAT 
GTGGGGCCCC AGCTTTCTCC ACAACAGATT GCCATGCTGA GCCAGCTTCC ACAGATTCCC 
CAGTTTCAAT TAGCATGTCA GCTCCTCCTT CAGCAGCAGC AGCAGCAGCA GCAGCTGTTA 
CAGAGCCAGA GGAAGTTATC TCAAGCTGTG CGACAGCAGC AGGAGCAACA GATTGCTCGT 
ATGGTGAGTG CCCTCCAGCA GCAGCAGCAG AGGCAGCCTG GCATGAAGCA TTCACCATCC 
CACCCTGTTG GGCCTAAGCC ACATATAGAC AGCATGGTAC CCAATCCTTT GAATGTGGGT 
CTTTCAGATT TACAGACCAA AGGACAAATA CCTGGATACG GTTCAGGCTT TGGCTCAAGC 
AGCATGGATT ATGGCATGGT GGGAGGGAAA GAAGCTGGAA CAGAATCCCG CTTTAAACAG 
TGGACTATGA TGGAAGGGCT GCCCTCTGTG GCTTCACAAG ATGCCAATAT GCACAAAAAT 
GGTGCAATAG TGCCCCCTGG AAAGGCTCGC GGAGGATCGC CCTACAACCA GTTCGACATA 
ATTCAGGGCG ACCCACTAGG TGGCCATGCA GGCCCTGCTG GTGATAGTTG GTTACCTGCC 
AAATCTCCAC CGACAAACAA GATCGGAAGC AAATCCAGCA ACGCCAGCTG GCCTCCAGAG 
TTCCAACCAG GAGTGCCATG GAAAGGTATA CAAAACATTG ACCCTGAATC TGACCCCTAC 
GTGACCCCTG GAAGTGTGCT GGGGGGGACA GCCACATCTC CCATTGTAGA TACTGACCAC 
CAACTTCTGC GGGACAACAC CACAGGGTCT AATTCTTCCC TCAACACCTC GCTGCCTTCA 
CCTGGTGCCT GGCCCTACAG TGCCTCTGAC AATTCCTTCA CCAACGTTCA TAGCACTTCA 
GCAAAATTCA GTGATTACAA ATCAACATGG TCCCCAGATC CCATAGGACA CAATCCCACT 
CATCTCTCCA ACAAGATGTG GAAAAACCAT ATTTCCTCAA GGAACACTAC AGGGCTGCCT 
CGCCCGCCTC CTGGCCTGAC CAACCCCAAA CCATCATCTC CATGGAACAG CACAGCACCC 
CGATCGGTCA GGGGCTGGGG GGCACAGGAC TCAAGGCTTG CCTCTGCATC TACTTGGAGT 
GATGGTAGCT CAGTTCGTCC TAGCTACTGG CTGGTTCTTC ACAATCTCAC TCCACAGATC 
GATGGGTCAA CCTTGAGGAC GATCTGCATG CAGCATGGTC CACTGCTGAC ATTCCATCTG 
AACCTGACCC AGGGCACCGC TCTCATCCGA TACAACACCA AACAGGAGGC GGCCAAGGCC 
CAGACTGCAC TGCACATGTG TGTGTTGGGA AACACTACCA TCCTTGCTGA GTTTGCCACA 
GATGAAGAGG TTAGTCGCTT TTTGACACAA GCTCAGCCCC CTACACCTGC AGCAACCCCA 
AGTGCACCCA CGGCAGGCTG GCAATCCCTG GAGACAGGAC AGAACCAGAC AGATCCAGTT 
GGACCTGCTT TGAATCTCTT TGGTGGGTCA ACAGGGCTTG GGCAGTGGAG CAGTGGTGGC 
GGCGGCAGCA GTGGGGGTGA TCTCACTGGC GCTTCGTTGT GGGGGCCCCC AAACTATTCT 
TCAAGCTTGT GGGGGGTCCC AAGCGTAGAG GATCCACACA GAATGGGCAG CCCTGCTCCC 
TTACTACCTG GAGACCTTCT GGGAGGAGGG TCGGATTCAA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004937788.2
CDS	544..5808
Translation	MQTDEGEILEEGSPKVSEMEHEDFVMEGHGKTPPPGEENKQEKEQEREEQLMEDKKRKKEDKKKKESAQKVTDQKTKVPEVTKPSLSQPVAASPIGNSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPPTAPGANPNNAQPVTGALLQSDSGTATDSTIGGAAASNYANSTWGPGASSSSGTNANPTHIWDKVIVDGSDMEEWPCIASKDAESSSENTTDNNSASNPGLEKNTLPGSTTSNKGKGSQCQSGSAGNECNLGAWKSDPKAKSVQSSNPATESNSGLSNWRNLTGQDRIGSGSGFSNFNPNSNPSAWPALVQEGNSRKGTSESDSGSSNAQISTVGQTSREQQSKMENAGVNFVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFEMGLGNPSRSTDTPSQSTGERKTGSVGSWGTARGSSGTDTVSGQSNSGNHGNNGKDREDSWKGASVQKPNGSRSDSWDNNNRSTGGGSWNFGPQHANESKWGEGNKLTSGVSQGEWKQLSGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNSQAPCWGRSSSSAGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEMPRSEGKSDKGTEGWESSATQTKNSGGWGDVPSQSNQIKSGWGELSTPTEWKDPKNTGGWNDYKNPNSSNWGGGRQEEKSSSSWNDSSSKDQGWGGRQPNQGWSSGKNGWGEEVDQAKNSNWESAGNKPASGWGEGGQNEIGTWGNGANTGSASKGGWDDCKRNSTWNETGRQPNSWNKQQQQQQQQQQETSGSWGPPPQTPSNGRPPNPNWNSGPQPAAPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPSSYNYKNVNLWDKNSQGGQAPREQSLPTPMTSKSQASGSKSMQDGWGESDGPVTGTRHSSWEEEEEGGVWNTAGSQGSSSSHNSTSWGQGGKKTQMKCSLKGGNSDSWMNPISKQFSNMGLLSQTEDIPGNKMDLSVGGLPDKKFEVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGMHTPVQPLNPSPNIRAQVPPQFLSPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQQLLQSQRKLSQAVRQQQEQQIARMVSALQQQQQRQPGMKHSPSHPVGPKPHIDSMVPNPLNVGLSDLQTKGQIPGYGSGFGSSSMDYGMVGGKEAGTESRFKQWTMMEGLPSVASQDANMHKNGAIVPPGKARGGSPYNQFDIIQGDPLGGHAGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFSDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTGLPRPPPGLTNPKPSSPWNSTAPRSVRGWGAQDSRLASASTWSDGSSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYNTKQEAAKAQTALHMCVLGNTTILAEFATDEEVSRFLTQAQPPTPAATPSAPTAGWQSLETGQNQTDPVGPALNLFGGSTGLGQWSSGGGGSSGGDLTGASLWGPPNYSSSLWGVPSVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_004937731.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus trinucleotide repeat containing 6B (TNRC6B), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004937731.3

ORF Insert Sequence:

ATGCAAACTG ATGAGGGAGA AATACTGGAG GAAGGCAGTC CCAAGGTCAG TGAGATGGAA 
CATGAGGATT TTGTAATGGA GGGGCATGGC AAGACTCCAC CTCCTGGTGA AGAAAACAAA 
CAAGAGAAGG AGCAAGAAAG GGAAGAACAG TTAATGGAAG ACAAGAAAAG GAAGAAGGAG 
GATAAGAAAA AGAAGGAATC TGCTCAGAAG GTCACAGATC AAAAAACCAA AGTGCCCGAA 
GTGACGAAAC CAAGTTTAAG CCAACCAGTG GCTGCCAGCC CAATTGGCAA CTCTCCATCG 
CCACCAGTCA ATGGTGGCAA CAATGCCAAA AGGGTGGCAG TGCCGAACGG ACAACCGCCA 
AGCGCCGCCC GCTACATGCC TCGGGAGGTG CCGCCGCGAT TCCGTTGCCA GCAGGACCAC 
AAAGTGTTAC TGAAACGTGG GCAGCCCCCT CCACCATCCT GTATGCTCCT TGGGGGTGGA 
GCAGGGCCTC CTCCCCCTAC AGCACCAGGA GCAAACCCAA ACAACGCACA ACCAGTGACA 
GGAGCACTGC TGCAGAGCGA CAGTGGGACT GCAACAGATT CAACAATTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGT CCTGGAGCCT CCTCCAGCAG CGGCACCAAC 
GCCAACCCAA CTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGTATCG CCAGCAAAGA TGCTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCGAACC CTGGCTTGGA GAAGAACACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGAAGCC AGTGCCAGTC TGGAAGCGCT GGAAATGAAT GTAATCTTGG GGCCTGGAAA 
TCTGATCCCA AGGCTAAATC TGTTCAATCT TCCAACCCTG CTACCGAGAG TAACAGTGGA 
CTAAGTAATT GGAGGAACTT GACTGGACAA GATAGAATTG GTTCTGGTTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCGTCTGCT TGGCCAGCAC TGGTCCAAGA GGGCAATTCT 
AGGAAAGGGA CTTCGGAATC TGATAGTGGT AGCTCCAATG CACAGATTAG CACAGTAGGT 
CAGACCTCTA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAACTT TGTCTCTGGC 
AGAGAACAGG CTCAAATTCA TAACACTGAT GGACCAAAAA ATGGAAACAC TAACTCCTTG 
AACTTAAGTT CACCAAACCC TATGGAGAAT AAGGGAATGC CCTTTGAAAT GGGCTTGGGG 
AACCCTTCCA GGAGCACTGA CACCCCTTCA CAAAGCACTG GAGAAAGAAA GACTGGGAGT 
GTTGGATCTT GGGGTACAGC TAGGGGGTCT TCTGGAACTG ACACAGTCTC TGGACAGAGC 
AATTCTGGAA ACCATGGGAA CAATGGAAAG GATAGGGAGG ACTCCTGGAA AGGAGCTTCT 
GTTCAAAAAC CTAATGGGTC AAGAAGCGAT TCTTGGGATA ACAATAACCG GTCTACGGGT 
GGTGGGTCTT GGAACTTTGG CCCTCAGCAT GCAAATGAAA GCAAATGGGG TGAAGGGAAC 
AAATTAACAT CTGGGGTCTC TCAGGGAGAA TGGAAACAGC TGTCTGGGTC TGATGAACTG 
AAGATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAAAAAGGCC ACCCTCTTCC TGAAAACCAA GGCAATTCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CTGCAGGAAG TGAGGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGACGC CGATCATACA GGCCTACGCA TCCTGATTGC 
CAGGCAGTCT TGCAGACTTT GCTGAGCAGG ACTGATTTGG ACCCCCGAGT GCTTTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAA GATACCGTAT GGGATATTGA AGAGATGCCA 
CGGTCCGAGG GGAAGTCTGA TAAAGGAACT GAGGGGTGGG AGAGCTCTGC CACACAGACA 
AAGAACTCAG GGGGCTGGGG CGATGTACCC AGCCAAAGCA ATCAAATCAA GTCTGGATGG 
GGTGAGCTCT CAACCCCAAC AGAATGGAAG GACCCCAAAA ATACTGGAGG ATGGAATGAC 
TATAAGAACC CCAATTCGTC TAATTGGGGA GGGGGAAGAC AAGAAGAGAA ATCGTCATCT 
TCTTGGAATG ACAGCTCTAG TAAGGATCAG GGGTGGGGTG GACGGCAGCC TAATCAAGGA 
TGGTCTTCTG GAAAGAATGG TTGGGGAGAG GAAGTTGACC AAGCAAAAAA CAGCAACTGG 
GAAAGTGCGG GAAATAAGCC AGCATCTGGT TGGGGTGAAG GTGGGCAAAA TGAGATTGGA 
ACTTGGGGTA ACGGTGCAAA TACAGGCTCT GCTTCAAAGG GTGGTTGGGA TGATTGTAAA 
AGAAATTCAA CGTGGAACGA GACGGGTCGA CAGCCCAACT CCTGGAACAA GCAGCAGCAG 
CAGCAACAGC AGCAACAGCA AGAGACTTCT GGTTCCTGGG GTCCACCGCC ACAAACTCCA 
AGTAATGGGC GACCTCCAAA CCCAAACTGG AACAGCGGAC CACAACCAGC TGCCCCCAAA 
GACGAGGAGC CTAGTGGCTG GGAAGAACCT TCTCCACAGT CAATCAGTCG AAAAATGGAT 
ATTGATGATG GCACTTCAGC GTGGGGAGAT CCTAGCAGTT ATAACTACAA GAATGTGAAC 
CTGTGGGACA AGAACTCACA AGGGGGACAG GCCCCACGGG AACAGAGCCT GCCTACTCCA 
ATGACTAGCA AATCACAAGC ATCAGGTTCT AAATCTATGC AAGATGGCTG GGGAGAGAGT 
GATGGGCCAG TGACAGGAAC ACGTCATTCC AGCTGGGAAG AGGAGGAGGA GGGAGGAGTC 
TGGAACACAG CAGGCTCTCA GGGAAGCAGT TCCTCCCACA ACTCAACAAG CTGGGGGCAA 
GGAGGAAAGA AAACGCAGAT GAAGTGCTCG TTAAAAGGAG GAAATAGTGA TTCATGGATG 
AACCCTATAT CCAAGCAGTT TTCAAATATG GGCTTGCTAA GTCAAACTGA GGACATTCCA 
GGCAATAAGA TGGACTTGTC TGTAGGTGGT CTCCCAGATA AGAAGTTTGA GGTAGATAAA 
CGAGCCATGA ATCTCGGGGA TTTTAATGAC ATAATGAGAA AGGATCGATC TGGGTTCCGT 
CCACCTAATT CCAAAGACAT GGGAACCACA GATAGTGGGC CTTATTTTGA GAAGGGTGGC 
AATCATGGTT TGTTTGGAAA CAGCACAGCA CAATCGAGGG GCATGCACAC ACCAGTGCAG 
CCACTAAATC CTTCCCCCAA TATCCGGGCG CAAGTGCCTC CCCAATTTCT TTCTCCCCAG 
GTTTCGGCCT CCATGCTCAA ACAGTTCCCC AACAGTGGCT TGAATCCAGG TCTTTTCAAT 
GTGGGGCCCC AGCTTTCTCC ACAACAGATT GCCATGCTGA GCCAGCTTCC ACAGATTCCC 
CAGTTTCAAT TAGCATGTCA GCTCCTCCTT CAGCAGCAGC AGCAGCAGCA GCAGCTGTTA 
CAGAGCCAGA GGAAGTTATC TCAAGCTGTG CGACAGCAGC AGGAGCAACA GATTGCTCGT 
ATGGTGAGTG CCCTCCAGCA GCAGCAGCAG AGGCAGCCTG GCATGAAGCA TTCACCATCC 
CACCCTGTTG GGCCTAAGCC ACATATAGAC AGCATGGTAC CCAATCCTTT GAATGTGGGT 
CTTTCAGATT TACAGACCAA AGGACAAATA CCTGGATACG GTTCAGGCTT TGGCTCAAGC 
AGCATGGATT ATGGCATGGT GGGAGGGAAA GAAGCTGGAA CAGAATCCCG CTTTAAACAG 
TGGACTATGA TGGAAGGGCT GCCCTCTGTG GCTTCACAAG ATGCCAATAT GCACAAAAAT 
GGTGCAATAG TGCCCCCTGG AAAGGCTCGC GGAGGATCGC CCTACAACCA GTTCGACATA 
ATTCAGGGCG ACCCACTAGG TGGCCATGCA GGCCCTGCTG GTGATAGTTG GTTACCTGCC 
AAATCTCCAC CGACAAACAA GATCGGAAGC AAATCCAGCA ACGCCAGCTG GCCTCCAGAG 
TTCCAACCAG GAGTGCCATG GAAAGGTATA CAAAACATTG ACCCTGAATC TGACCCCTAC 
GTGACCCCTG GAAGTGTGCT GGGGGGGACA GCCACATCTC CCATTGTAGA TACTGACCAC 
CAACTTCTGC GGGACAACAC CACAGGGTCT AATTCTTCCC TCAACACCTC GCTGCCTTCA 
CCTGGTGCCT GGCCCTACAG TGCCTCTGAC AATTCCTTCA CCAACGTTCA TAGCACTTCA 
GCAAAATTCA GTGATTACAA ATCAACATGG TCCCCAGATC CCATAGGACA CAATCCCACT 
CATCTCTCCA ACAAGATGTG GAAAAACCAT ATTTCCTCAA GGAACACTAC AGGGCTGCCT 
CGCCCGCCTC CTGGCCTGAC CAACCCCAAA CCATCATCTC CATGGAACAG CACAGCACCC 
CGATCGGTCA GGGGCTGGGG GGCACAGGAC TCAAGGCTTG CCTCTGCATC TACTTGGAGT 
GATGGTAGCT CAGTTCGTCC TAGCTACTGG CTGGTTCTTC ACAATCTCAC TCCACAGATC 
GATGGGTCAA CCTTGAGGAC GATCTGCATG CAGCATGGTC CACTGCTGAC ATTCCATCTG 
AACCTGACCC AGGGCACCGC TCTCATCCGA TACAACACCA AACAGGAGGC GGCCAAGGCC 
CAGACTGCAC TGCACATGTG TGTGTTGGGA AACACTACCA TCCTTGCTGA GTTTGCCACA 
GATGAAGAGG TTAGTCGCTT TTTGACACAA GCTCAGCCCC CTACACCTGC AGCAACCCCA 
AGTGCACCCA CGGCAGGCTG GCAATCCCTG GAGACAGGAC AGAACCAGAC AGATCCAGTT 
GGACCTGCTT TGAATCTCTT TGGTGGGTCA ACAGGGCTTG GGCAGTGGAG CAGTGGTGGC 
GGCGGCAGCA GTGGGGGTGA TCTCACTGGC GCTTCGTTGT GGGGGCCCCC AAACTATTCT 
TCAAGCTTGT GGGGGGTCCC AAGCGTAGAG GATCCACACA GAATGGGCAG CCCTGCTCCC 
TTACTACCTG GAGACCTTCT GGGAGGAGGG TCGGATTCAA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TNRC6B
Entrez Gene ID	418010
Full Name	trinucleotide repeat containing 6B
General protein information	Preferred Names trinucleotide repeat containing 6B Names trinucleotide repeat-containing gene 6B protein
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

	TNRC6B	XP_001101111.2
Canis lupus familiaris (dog)	TNRC6B	XP_005625909.1
Bos taurus (cattle)	TNRC6B	NP_001179584.1
Gallus gallus (chicken)	TNRC6B	XP_004937779.1
Danio rerio (zebrafish)	tnrc6b	XP_002663990.3
Xenopus tropicalis (tropical clawed frog)	tnrc6b	XP_002934255.2
Homo sapiens (human)	TNRC6B	NP_001155973.1
Pan troglodytes (chimpanzee)	TNRC6B	XP_003317299.1
Mus musculus (house mouse)	Tnrc6b	NP_659061.2
Rattus norvegicus (Norway rat)	Tnrc6b	NP_620200.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TNRC6B cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

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