GIT1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	GIT1
Entrez Gene ID	417584
Full Name	G protein-coupled receptor kinase interacting ArfGAP 1
Synonyms	p95-APP1
General protein information	Preferred Names G protein-coupled receptor kinase interacting ArfGAP 1 Names ARF GTPase-activating protein GIT1 ArfGAP-putative, Pix-interacting, paxillin-interacting protein 1 G protein-coupled receptor kinase-interactor 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015295768.1	XP_015151254.1	ARF GTPase-activating protein GIT1 isoform X1
XM_015295769.1	XP_015151255.1	ARF GTPase-activating protein GIT1 isoform X2
XM_015295769.2	XP_015151255.1	ARF GTPase-activating protein GIT1 isoform X2
XM_015295768.2	XP_015151254.1	ARF GTPase-activating protein GIT1 isoform X1
XM_015295770.1	XP_015151256.1	ARF GTPase-activating protein GIT1 isoform X3
NM_204296.1	NP_989627.1	ARF GTPase-activating protein GIT1
XM_015295770.2	XP_015151256.1	ARF GTPase-activating protein GIT1 isoform X3

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC568935	XP_697386.4
Mus musculus (house mouse)	Git1	NP_001004144.1
Gallus gallus (chicken)	GIT1	NP_989627.1
Homo sapiens (human)	GIT1	NP_001078923.1
	GIT1	XP_001108023.2
Rattus norvegicus (Norway rat)	Git1	NP_114002.1
Pan troglodytes (chimpanzee)	GIT1	XP_511377.3
Canis lupus familiaris (dog)	GIT1	XP_005625057.1
Bos taurus (cattle)	GIT1	XP_002695750.3

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of the endogenous GIT1-betaPIX complex, and identification of its association to membranes.
Botrugno OA, Paris S, Za L, Gualdoni S, Cattaneo A, Bachi A, de Curtis I
European journal of cell biology85(1)35-46(2006 Jan)

p95-APP1 links membrane transport to Rac-mediated reorganization of actin.
Di Cesare A, Paris S, Albertinazzi C, Dariozzi S, Andersen J, Mann M, Longhi R, de Curtis I
Nature cell biology2(8)521-30(2000 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our findings show the specific localization of the complex at intracellular membranes, and indicate a correlation between the association of GIT1 to betaPIX, and the localization of the endogenous complex at membranes.
Title: Characterization of the endogenous GIT1-betaPIX complex, and identification of its association to membranes.

The following GIT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GIT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa25901	NM_204296.1 Latest version!	Gallus gallus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OGa44316	XM_015295768.2 Latest version!	Gallus gallus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OGa44317	XM_015295769.2 Latest version!	Gallus gallus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OGa44318	XM_015295770.2 Latest version!	Gallus gallus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
View Old Accession Versions >>
OGa44317	XM_015295769.1	Gallus gallus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OGa44316	XM_015295768.1	Gallus gallus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OGa44318	XM_015295770.1	Gallus gallus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GIT1 ( NM_204296.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_989627.1 Gallus gallus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), mRNA.

Price & Availability↑

CloneID	OGa25901
Clone ID Related Accession (Same CDS sequence)	NM_204296.1
Accession Version	NM_204296.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-20
Organism	Gallus gallus(chicken)
Product	ARF GTPase-activating protein GIT1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF216970.1. On Nov 16, 2011 this sequence version replaced XM_415830.2. ##Evidence-Data-START## Transcript exon combination :: AF216970.1 [ECO:0000332] ##Evidence-Data-END##

ATGTCCCGGA AGGCGCAGCG GGCGGAGGTG TGCGCGGACT GCAGTGCCGC AGACCCGGGC 
TGGGCCTCCA TCAACCGCGG TGTGCTCATC TGCGACGAGT GCTGCAGCGT GCACCGCAGC 
CTGGGCCGCC ACATCTCCAT CGTCAAGCAC CTGCGGCACA GCCCCTGGCC CGCCACGCTG 
CTGCAGATGG TGCACACCTT GGCCAGCAAT GGGGCCAACT CCATCTGGGA GCATTCGCTG 
CTGGACCCGG CGCAGGTGCA GAGCGGGCGC AGGAAGGCGA ACCCCCAGGA CAAAGTGCAC 
CCCACCAAGT CGGAGTTCAT CCGCGCCAAG TACCAGATGC TGGCCTTCGT GCACAAGCTG 
CCGTGCCGGG ATGACGACGG GGTCACCGCC AAGGACCTCA GCAAGCAATT GCACTCCAGC 
GTGCGGACGG GCAACCTGGA GACGTGCCTG CGCCTGCTCT CGCTGGGGGC TCAGGCCAAC 
TTCTTCCACC CGGAGAAGGG CACCACGCCG CTGCACGTGG CTGCCAAGGC CGGGCAGATC 
CTGCAGGCGG AGCTGCTGGT GGTGTACGGC GCTGACCCCG GTGCGCCCGA CGTCAACGGG 
CGGACACCCA TCGACTACGC CAGGCAGGCG GCCCAGCACG AGCTGGCGGA GCGGCTGGTG 
GAGTGCCAGT ATGAGCTGAC CGACCGGCTG GCCTTCTACC TGTGCGGCAG GAAGCCGGAC 
CACAAGAACG GGCACTACAT CATCCCGCAG ATGGCAGACA GCCTGGACCT GTCCGAGCTG 
GCCAAGGCGG CCAAGAAGAA GCTGCAAGCG CTCAGCAACC GCCTGTTTGA GGAGCTGGCC 
ATGGATGTGT ACGACGAGGT GGATCGGCGC GAGAACGACG CGGTGTGGCT GACGACGCAG 
AACCACAGCA CGTTGGTGAC CGAGCGCAGC GCCGTCCCCT TCCTCCCCGT CAACCCCGAG 
TACTCCGCCA CCCGCAACCA GGGCCGGCAG AAGTTGGCCA GGTTCAACGC CCGGGAGTTT 
GCCACTCTGC TCATCGATAT CCTGGGGGAA GCCAAGCGCC GGCAGCAGGG GAAGAGCCCG 
CTCAGCCCCA CGGATGCCCT CGACTATTCG CTGAGGAGCC AGAGTGACCT GGACGACCAG 
CACGACTACG ACAGCGTCGC CTCCGACGAG GACACGGACC AGGAGCTGCT GCGCAACGCG 
TCCCGCAACA ACCGCGCACG GAGCATGGAC TCCTCTGACC TGTCGGACGG CCCCATCACG 
CTGCAGGAGT ACCTGGAGGT GAAGAAAGCC CTGGCCGCCT CCGAGGCCAA AGTGCAGCAG 
CTGATGAAGG TGAACAACAG CCTGAGCGAT GAGCTGCGCC GGCTGCAGCG CGAGATCCAC 
AAGCTGCAAT CAGAGAACAC GCAGATCCGG CAGCAGGCGG TGCCCCCACA CCCCACCCCA 
GCCCCCAGCG AGCGCCCCGA GCACGGGCAC CCCCCCGGCA CGGCCCCCCT GCACCGCCGT 
GACCGCCAGG CCTTCTCCAT GTACGAGCCG GGCTCGGTGC TGAAACCCTT TGGGCAGCCG 
GTGGAGGAGC TGGTGACCCG GCTGCAGCCC TTCGGAGGCG GCATGCGGAA GGGTCCGTCG 
GTCTCCTCCA TGCCTTTTCC CCCATCCTCC CCGCTGCTCT CCTGCCCACC CGATGGTGCC 
CGGCACATGA GCAAACTGGA CCGGCACGGC AGCGGCACCG ACAGCGACTA CGATAACACG 
CAGACGGGCG AGATCCTGCT GGGCGTGGAG GGGAAGCGCT TCGTGGAGCT GAGCAAGGAC 
GAGGACTTCC CCCACGAGCT GGACCCGCTG GACGGGGAGC TGGACCCCGG GCTGCCCAGC 
ACGGAGGACG TCATCCTGAA AACCGAGCAG GTCACCAAAA ACATCCAGGA GCTGCTGCGG 
GCTGCGCAGG AGTCCAAACA CGACAGCTTC GTGCCGTGCT CCGAGAAGAT CCACTCAGCC 
GTGACGGAGA TGGCGTCGCT CTTCCCCAAG AAACCGGCGC TGGAGACGGT GCGGAGCTCC 
CTGCGCCTGC TCAACGCCAG CGCCTACCGG CTGCAGAGCG AGTGCCGCAA AACCGTGCCG 
CCGGAGCCGG GCGCAGCCGT GGACTACCAG CTGCTGACAC AGCAGGTCAT CCAGTGCGCC 
TACGACATCG CCAAGGCGGC CAAGCAGTTG GTCACCATCA CCACCCGCGA GAAGAAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989627.1
CDS	16..2238
Translation	MSRKAQRAEVCADCSAADPGWASINRGVLICDECCSVHRSLGRHISIVKHLRHSPWPATLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKVHPTKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQILQAELLVVYGADPGAPDVNGRTPIDYARQAAQHELAERLVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADSLDLSELAKAAKKKLQALSNRLFEELAMDVYDEVDRRENDAVWLTTQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLLIDILGEAKRRQQGKSPLSPTDALDYSLRSQSDLDDQHDYDSVASDEDTDQELLRNASRNNRARSMDSSDLSDGPITLQEYLEVKKALAASEAKVQQLMKVNNSLSDELRRLQREIHKLQSENTQIRQQAVPPHPTPAPSERPEHGHPPGTAPLHRRDRQAFSMYEPGSVLKPFGQPVEELVTRLQPFGGGMRKGPSVSSMPFPPSSPLLSCPPDGARHMSKLDRHGSGTDSDYDNTQTGEILLGVEGKRFVELSKDEDFPHELDPLDGELDPGLPSTEDVILKTEQVTKNIQELLRAAQESKHDSFVPCSEKIHSAVTEMASLFPKKPALETVRSSLRLLNASAYRLQSECRKTVPPEPGAAVDYQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	NM_204296.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204296.1

ORF Insert Sequence:

ATGTCCCGGA AGGCGCAGCG GGCGGAGGTG TGCGCGGACT GCAGTGCCGC AGACCCGGGC 
TGGGCCTCCA TCAACCGCGG TGTGCTCATC TGCGACGAGT GCTGCAGCGT GCACCGCAGC 
CTGGGCCGCC ACATCTCCAT CGTCAAGCAC CTGCGGCACA GCCCCTGGCC CGCCACGCTG 
CTGCAGATGG TGCACACCTT GGCCAGCAAT GGGGCCAACT CCATCTGGGA GCATTCGCTG 
CTGGACCCGG CGCAGGTGCA GAGCGGGCGC AGGAAGGCGA ACCCCCAGGA CAAAGTGCAC 
CCCACCAAGT CGGAGTTCAT CCGCGCCAAG TACCAGATGC TGGCCTTCGT GCACAAGCTG 
CCGTGCCGGG ATGACGACGG GGTCACCGCC AAGGACCTCA GCAAGCAATT GCACTCCAGC 
GTGCGGACGG GCAACCTGGA GACGTGCCTG CGCCTGCTCT CGCTGGGGGC TCAGGCCAAC 
TTCTTCCACC CGGAGAAGGG CACCACGCCG CTGCACGTGG CTGCCAAGGC CGGGCAGATC 
CTGCAGGCGG AGCTGCTGGT GGTGTACGGC GCTGACCCCG GTGCGCCCGA CGTCAACGGG 
CGGACACCCA TCGACTACGC CAGGCAGGCG GCCCAGCACG AGCTGGCGGA GCGGCTGGTG 
GAGTGCCAGT ATGAGCTGAC CGACCGGCTG GCCTTCTACC TGTGCGGCAG GAAGCCGGAC 
CACAAGAACG GGCACTACAT CATCCCGCAG ATGGCAGACA GCCTGGACCT GTCCGAGCTG 
GCCAAGGCGG CCAAGAAGAA GCTGCAAGCG CTCAGCAACC GCCTGTTTGA GGAGCTGGCC 
ATGGATGTGT ACGACGAGGT GGATCGGCGC GAGAACGACG CGGTGTGGCT GACGACGCAG 
AACCACAGCA CGTTGGTGAC CGAGCGCAGC GCCGTCCCCT TCCTCCCCGT CAACCCCGAG 
TACTCCGCCA CCCGCAACCA GGGCCGGCAG AAGTTGGCCA GGTTCAACGC CCGGGAGTTT 
GCCACTCTGC TCATCGATAT CCTGGGGGAA GCCAAGCGCC GGCAGCAGGG GAAGAGCCCG 
CTCAGCCCCA CGGATGCCCT CGACTATTCG CTGAGGAGCC AGAGTGACCT GGACGACCAG 
CACGACTACG ACAGCGTCGC CTCCGACGAG GACACGGACC AGGAGCTGCT GCGCAACGCG 
TCCCGCAACA ACCGCGCACG GAGCATGGAC TCCTCTGACC TGTCGGACGG CCCCATCACG 
CTGCAGGAGT ACCTGGAGGT GAAGAAAGCC CTGGCCGCCT CCGAGGCCAA AGTGCAGCAG 
CTGATGAAGG TGAACAACAG CCTGAGCGAT GAGCTGCGCC GGCTGCAGCG CGAGATCCAC 
AAGCTGCAAT CAGAGAACAC GCAGATCCGG CAGCAGGCGG TGCCCCCACA CCCCACCCCA 
GCCCCCAGCG AGCGCCCCGA GCACGGGCAC CCCCCCGGCA CGGCCCCCCT GCACCGCCGT 
GACCGCCAGG CCTTCTCCAT GTACGAGCCG GGCTCGGTGC TGAAACCCTT TGGGCAGCCG 
GTGGAGGAGC TGGTGACCCG GCTGCAGCCC TTCGGAGGCG GCATGCGGAA GGGTCCGTCG 
GTCTCCTCCA TGCCTTTTCC CCCATCCTCC CCGCTGCTCT CCTGCCCACC CGATGGTGCC 
CGGCACATGA GCAAACTGGA CCGGCACGGC AGCGGCACCG ACAGCGACTA CGATAACACG 
CAGACGGGCG AGATCCTGCT GGGCGTGGAG GGGAAGCGCT TCGTGGAGCT GAGCAAGGAC 
GAGGACTTCC CCCACGAGCT GGACCCGCTG GACGGGGAGC TGGACCCCGG GCTGCCCAGC 
ACGGAGGACG TCATCCTGAA AACCGAGCAG GTCACCAAAA ACATCCAGGA GCTGCTGCGG 
GCTGCGCAGG AGTCCAAACA CGACAGCTTC GTGCCGTGCT CCGAGAAGAT CCACTCAGCC 
GTGACGGAGA TGGCGTCGCT CTTCCCCAAG AAACCGGCGC TGGAGACGGT GCGGAGCTCC 
CTGCGCCTGC TCAACGCCAG CGCCTACCGG CTGCAGAGCG AGTGCCGCAA AACCGTGCCG 
CCGGAGCCGG GCGCAGCCGT GGACTACCAG CTGCTGACAC AGCAGGTCAT CCAGTGCGCC 
TACGACATCG CCAAGGCGGC CAAGCAGTTG GTCACCATCA CCACCCGCGA GAAGAAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT1 ( XM_015295768.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015151254.1 Gallus gallus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa44316
Clone ID Related Accession (Same CDS sequence)	XM_015295768.1 , XM_015295768.2
Accession Version	XM_015295768.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ARF GTPase-activating protein GIT1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455988.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCGGA AGGCGCAGCG GGCGGAGGTG TGCGCGGACT GCAGTGCCGC AGACCCGGGC 
TGGGCCTCCA TCAACCGCGG TGTGCTCATC TGCGACGAGT GCTGCAGCGT GCACCGCAGC 
CTGGGCCGCC ACATCTCCAT CGTCAAGCAC CTGCGGCACA GCCCCTGGCC CGCCACGCTG 
CTGCAGATGG TGCACACCTT GGCCAGCAAT GGGGCCAACT CCATCTGGGA GCATTCGCTG 
CTGGACCCGG CGCAGGTGCA GAGCGGGCGC AGGAAGGCGA ACCCCCAGGA CAAAGTGCAC 
CCCACCAAGT CGGAGTTCAT CCGCGCCAAG TACCAGATGC TGGCCTTCGT GCACAAGCTG 
CCGTGCCGGG ATGACGACGG GGTCACCGCC AAGGACCTCA GCAAGCAATT GCACTCCAGC 
GTGCGGACGG GCAACCTGGA GACGTGCCTG CGCCTGCTCT CGCTGGGGGC TCAGGCCAAC 
TTCTTCCACC CGGAGAAGGG CACCACGCCG CTGCACGTGG CTGCCAAGGC CGGGCAGATC 
CTGCAGGCAG AGCTGCTGGT GGTGTACGGC GCTGACCCCG GTGCACCCGA CGTCAACGGG 
CGGACACCCA TCGACTACGC CAGGCAGGCG GCCCAGCACG AGCTGGCGGA GCGGCTGGTG 
GAGTGCCAGT ATGAGCTGAC CGACCGGCTG GCCTTCTACC TGTGCGGCAG GAAGCCGGAC 
CACAAGAACG GGCACTACAT CATCCCGCAG ATGGCAGACA GCCTGGACCT GTCCGAGCTG 
GCCAAGGCGG CCAAGAAGAA GCTGCAAGCG CTCAGCAACC GCCTGTTTGA GGAGCTGGCC 
ATGGATGTGT ACGATGAGGT GGATCGGCGC GAGAACGACG CGGTGTGGCT GACGACGCAG 
AACCACAGCA CGTTGGTGAC CGAGCGCAGC GCCGTCCCCT TCCTCCCCGT CAACCCCGAG 
TACTCCGCCA CCCGCAACCA GGGCCGGCAG AAGTTGGCCA GGTTCAACGC CCGGGAGTTT 
GCCACTCTGC TCATCGATAT CCTGGGGGAA GCCAAGCGCC GGCAGCAGGG GAAGAGCCCG 
CTCAGCCCCA CGGATGCCCT CGACTATTCG CTGAGGAGCC AGAGTGACCT GGACGACCAG 
CACGACTACG ACAGCGTCGC CTCCGACGAG GACACGGACC AGGAGCTGCT GCGCAACGCG 
TCCCGCAACA ACCGCGCACG GAGCATGGAC TCCTCTGACC TGTCGGACGG CCCCATCACG 
CTGCAGGAGT ACCTGGAGGT GAAGAAAGCC CTGGCCGCCT CCGAGGCCAA AGTGCAGCAG 
CTGATGAAGG TGAACAACAG CCTGAGCGAT GAGCTGCGCC GGCTGCAGCG CGAGATCCAC 
AAGCTGCAAT CAGAGAACAC GCAGATCCGG CAGCAGGCGG TGCCCCCACA CCCCACCCCA 
GCCCCCAGCG AGCGCCCCGA GCACGGGCAC CCCCCCGGCA CGGCCCCCCT GCACCGCCGT 
GACCGCCAGG CCTTCTCCAT GTACGAGCCG GGCTCGGTGC TGAAACCCTT TGGGCAGCCG 
GTGGAGGAGC TGGTGACCCG GCTGCAGCCC TTCGGAGGCG GCGAGGTGGA GGACGAAGCT 
CTGTACTCCA TGCACATCCC GGCCAGCGTG TACCGGATGC GGAAGGGTCC GTCGGTCTCC 
TCCATGCCTT TTCCCCCATC CTCCCCGCTG CTCTCCTGCC CACCCGATGG TGCCCGGCAC 
ATGAGCAAAC TGGACCGGCA CGGCAGCGGC ACCGACAGCG ACTACGATAA CACGCAGACG 
GGCGAGATCC TGCTGGGCGT GGAGGGGAAG CGCTTCGTGG AGCTGAGCAA GGACGAGGAC 
TTCCCCCACG AGCTGGACCC GCTGGACGGG GAGCTGGACC CCGGGCTGCC CAGCACGGAG 
GATGTCATCC TGAAAACCGA GCAGGTCACC AAAAACATCC AGGAGCTGCT GCGGGCTGCG 
CAGGAGTCCA AACACGACAG CTTCGTGCCG TGCTCCGAGA AGATCCACTC AGCCGTGACG 
GAGATGGCGT CGCTCTTCCC CAAGAAACCG GCGCTGGAGA CGGTGCGGAG CTCCCTGCGC 
CTGCTCAATG CCAGCGCCTA CCGGCTGCAG AGCGAGTGCC GCAAAACCGT GCCGCCGGAG 
CCGGGCGCAG CCGTGGACTA CCAGCTGCTG ACACAGCAGG TCATCCAGTG CGCCTACGAC 
ATCGCCAAGG CGGCCAAGCA GTTGGTCACC ATCACCACCC GCGAGAAGAA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015151254.1
CDS	77..2353
Translation	MSRKAQRAEVCADCSAADPGWASINRGVLICDECCSVHRSLGRHISIVKHLRHSPWPATLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKVHPTKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQILQAELLVVYGADPGAPDVNGRTPIDYARQAAQHELAERLVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADSLDLSELAKAAKKKLQALSNRLFEELAMDVYDEVDRRENDAVWLTTQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLLIDILGEAKRRQQGKSPLSPTDALDYSLRSQSDLDDQHDYDSVASDEDTDQELLRNASRNNRARSMDSSDLSDGPITLQEYLEVKKALAASEAKVQQLMKVNNSLSDELRRLQREIHKLQSENTQIRQQAVPPHPTPAPSERPEHGHPPGTAPLHRRDRQAFSMYEPGSVLKPFGQPVEELVTRLQPFGGGEVEDEALYSMHIPASVYRMRKGPSVSSMPFPPSSPLLSCPPDGARHMSKLDRHGSGTDSDYDNTQTGEILLGVEGKRFVELSKDEDFPHELDPLDGELDPGLPSTEDVILKTEQVTKNIQELLRAAQESKHDSFVPCSEKIHSAVTEMASLFPKKPALETVRSSLRLLNASAYRLQSECRKTVPPEPGAAVDYQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_015295768.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015295768.1

ORF Insert Sequence:

ATGTCCCGGA AGGCGCAGCG GGCGGAGGTG TGCGCGGACT GCAGTGCCGC AGACCCGGGC 
TGGGCCTCCA TCAACCGCGG TGTGCTCATC TGCGACGAGT GCTGCAGCGT GCACCGCAGC 
CTGGGCCGCC ACATCTCCAT CGTCAAGCAC CTGCGGCACA GCCCCTGGCC CGCCACGCTG 
CTGCAGATGG TGCACACCTT GGCCAGCAAT GGGGCCAACT CCATCTGGGA GCATTCGCTG 
CTGGACCCGG CGCAGGTGCA GAGCGGGCGC AGGAAGGCGA ACCCCCAGGA CAAAGTGCAC 
CCCACCAAGT CGGAGTTCAT CCGCGCCAAG TACCAGATGC TGGCCTTCGT GCACAAGCTG 
CCGTGCCGGG ATGACGACGG GGTCACCGCC AAGGACCTCA GCAAGCAATT GCACTCCAGC 
GTGCGGACGG GCAACCTGGA GACGTGCCTG CGCCTGCTCT CGCTGGGGGC TCAGGCCAAC 
TTCTTCCACC CGGAGAAGGG CACCACGCCG CTGCACGTGG CTGCCAAGGC CGGGCAGATC 
CTGCAGGCAG AGCTGCTGGT GGTGTACGGC GCTGACCCCG GTGCACCCGA CGTCAACGGG 
CGGACACCCA TCGACTACGC CAGGCAGGCG GCCCAGCACG AGCTGGCGGA GCGGCTGGTG 
GAGTGCCAGT ATGAGCTGAC CGACCGGCTG GCCTTCTACC TGTGCGGCAG GAAGCCGGAC 
CACAAGAACG GGCACTACAT CATCCCGCAG ATGGCAGACA GCCTGGACCT GTCCGAGCTG 
GCCAAGGCGG CCAAGAAGAA GCTGCAAGCG CTCAGCAACC GCCTGTTTGA GGAGCTGGCC 
ATGGATGTGT ACGATGAGGT GGATCGGCGC GAGAACGACG CGGTGTGGCT GACGACGCAG 
AACCACAGCA CGTTGGTGAC CGAGCGCAGC GCCGTCCCCT TCCTCCCCGT CAACCCCGAG 
TACTCCGCCA CCCGCAACCA GGGCCGGCAG AAGTTGGCCA GGTTCAACGC CCGGGAGTTT 
GCCACTCTGC TCATCGATAT CCTGGGGGAA GCCAAGCGCC GGCAGCAGGG GAAGAGCCCG 
CTCAGCCCCA CGGATGCCCT CGACTATTCG CTGAGGAGCC AGAGTGACCT GGACGACCAG 
CACGACTACG ACAGCGTCGC CTCCGACGAG GACACGGACC AGGAGCTGCT GCGCAACGCG 
TCCCGCAACA ACCGCGCACG GAGCATGGAC TCCTCTGACC TGTCGGACGG CCCCATCACG 
CTGCAGGAGT ACCTGGAGGT GAAGAAAGCC CTGGCCGCCT CCGAGGCCAA AGTGCAGCAG 
CTGATGAAGG TGAACAACAG CCTGAGCGAT GAGCTGCGCC GGCTGCAGCG CGAGATCCAC 
AAGCTGCAAT CAGAGAACAC GCAGATCCGG CAGCAGGCGG TGCCCCCACA CCCCACCCCA 
GCCCCCAGCG AGCGCCCCGA GCACGGGCAC CCCCCCGGCA CGGCCCCCCT GCACCGCCGT 
GACCGCCAGG CCTTCTCCAT GTACGAGCCG GGCTCGGTGC TGAAACCCTT TGGGCAGCCG 
GTGGAGGAGC TGGTGACCCG GCTGCAGCCC TTCGGAGGCG GCGAGGTGGA GGACGAAGCT 
CTGTACTCCA TGCACATCCC GGCCAGCGTG TACCGGATGC GGAAGGGTCC GTCGGTCTCC 
TCCATGCCTT TTCCCCCATC CTCCCCGCTG CTCTCCTGCC CACCCGATGG TGCCCGGCAC 
ATGAGCAAAC TGGACCGGCA CGGCAGCGGC ACCGACAGCG ACTACGATAA CACGCAGACG 
GGCGAGATCC TGCTGGGCGT GGAGGGGAAG CGCTTCGTGG AGCTGAGCAA GGACGAGGAC 
TTCCCCCACG AGCTGGACCC GCTGGACGGG GAGCTGGACC CCGGGCTGCC CAGCACGGAG 
GATGTCATCC TGAAAACCGA GCAGGTCACC AAAAACATCC AGGAGCTGCT GCGGGCTGCG 
CAGGAGTCCA AACACGACAG CTTCGTGCCG TGCTCCGAGA AGATCCACTC AGCCGTGACG 
GAGATGGCGT CGCTCTTCCC CAAGAAACCG GCGCTGGAGA CGGTGCGGAG CTCCCTGCGC 
CTGCTCAATG CCAGCGCCTA CCGGCTGCAG AGCGAGTGCC GCAAAACCGT GCCGCCGGAG 
CCGGGCGCAG CCGTGGACTA CCAGCTGCTG ACACAGCAGG TCATCCAGTG CGCCTACGAC 
ATCGCCAAGG CGGCCAAGCA GTTGGTCACC ATCACCACCC GCGAGAAGAA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT1 ( XM_015295768.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015151254.1 Gallus gallus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa44316
Clone ID Related Accession (Same CDS sequence)	XM_015295768.1 , XM_015295768.2
Accession Version	XM_015295768.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ARF GTPase-activating protein GIT1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006106.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015295768.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCGGA AGGCGCAGCG GGCGGAGGTG TGCGCGGACT GCAGTGCCGC AGACCCGGGC 
TGGGCCTCCA TCAACCGCGG TGTGCTCATC TGCGACGAGT GCTGCAGCGT GCACCGCAGC 
CTGGGCCGCC ACATCTCCAT CGTCAAGCAC CTGCGGCACA GCCCCTGGCC CGCCACGCTG 
CTGCAGATGG TGCACACCTT GGCCAGCAAT GGGGCCAACT CCATCTGGGA GCATTCGCTG 
CTGGACCCGG CGCAGGTGCA GAGCGGGCGC AGGAAGGCGA ACCCCCAGGA CAAAGTGCAC 
CCCACCAAGT CGGAGTTCAT CCGCGCCAAG TACCAGATGC TGGCCTTCGT GCACAAGCTG 
CCGTGCCGGG ATGACGACGG GGTCACCGCC AAGGACCTCA GCAAGCAATT GCACTCCAGC 
GTGCGGACGG GCAACCTGGA GACGTGCCTG CGCCTGCTCT CGCTGGGGGC TCAGGCCAAC 
TTCTTCCACC CGGAGAAGGG CACCACGCCG CTGCACGTGG CTGCCAAGGC CGGGCAGATC 
CTGCAGGCAG AGCTGCTGGT GGTGTACGGC GCTGACCCCG GTGCACCCGA CGTCAACGGG 
CGGACACCCA TCGACTACGC CAGGCAGGCG GCCCAGCACG AGCTGGCGGA GCGGCTGGTG 
GAGTGCCAGT ATGAGCTGAC CGACCGGCTG GCCTTCTACC TGTGCGGCAG GAAGCCGGAC 
CACAAGAACG GGCACTACAT CATCCCGCAG ATGGCAGACA GCCTGGACCT GTCCGAGCTG 
GCCAAGGCGG CCAAGAAGAA GCTGCAAGCG CTCAGCAACC GCCTGTTTGA GGAGCTGGCC 
ATGGATGTGT ACGATGAGGT GGATCGGCGC GAGAACGACG CGGTGTGGCT GACGACGCAG 
AACCACAGCA CGTTGGTGAC CGAGCGCAGC GCCGTCCCCT TCCTCCCCGT CAACCCCGAG 
TACTCCGCCA CCCGCAACCA GGGCCGGCAG AAGTTGGCCA GGTTCAACGC CCGGGAGTTT 
GCCACTCTGC TCATCGATAT CCTGGGGGAA GCCAAGCGCC GGCAGCAGGG GAAGAGCCCG 
CTCAGCCCCA CGGATGCCCT CGACTATTCG CTGAGGAGCC AGAGTGACCT GGACGACCAG 
CACGACTACG ACAGCGTCGC CTCCGACGAG GACACGGACC AGGAGCTGCT GCGCAACGCG 
TCCCGCAACA ACCGCGCACG GAGCATGGAC TCCTCTGACC TGTCGGACGG CCCCATCACG 
CTGCAGGAGT ACCTGGAGGT GAAGAAAGCC CTGGCCGCCT CCGAGGCCAA AGTGCAGCAG 
CTGATGAAGG TGAACAACAG CCTGAGCGAT GAGCTGCGCC GGCTGCAGCG CGAGATCCAC 
AAGCTGCAAT CAGAGAACAC GCAGATCCGG CAGCAGGCGG TGCCCCCACA CCCCACCCCA 
GCCCCCAGCG AGCGCCCCGA GCACGGGCAC CCCCCCGGCA CGGCCCCCCT GCACCGCCGT 
GACCGCCAGG CCTTCTCCAT GTACGAGCCG GGCTCGGTGC TGAAACCCTT TGGGCAGCCG 
GTGGAGGAGC TGGTGACCCG GCTGCAGCCC TTCGGAGGCG GCGAGGTGGA GGACGAAGCT 
CTGTACTCCA TGCACATCCC GGCCAGCGTG TACCGGATGC GGAAGGGTCC GTCGGTCTCC 
TCCATGCCTT TTCCCCCATC CTCCCCGCTG CTCTCCTGCC CACCCGATGG TGCCCGGCAC 
ATGAGCAAAC TGGACCGGCA CGGCAGCGGC ACCGACAGCG ACTACGATAA CACGCAGACG 
GGCGAGATCC TGCTGGGCGT GGAGGGGAAG CGCTTCGTGG AGCTGAGCAA GGACGAGGAC 
TTCCCCCACG AGCTGGACCC GCTGGACGGG GAGCTGGACC CCGGGCTGCC CAGCACGGAG 
GATGTCATCC TGAAAACCGA GCAGGTCACC AAAAACATCC AGGAGCTGCT GCGGGCTGCG 
CAGGAGTCCA AACACGACAG CTTCGTGCCG TGCTCCGAGA AGATCCACTC AGCCGTGACG 
GAGATGGCGT CGCTCTTCCC CAAGAAACCG GCGCTGGAGA CGGTGCGGAG CTCCCTGCGC 
CTGCTCAATG CCAGCGCCTA CCGGCTGCAG AGCGAGTGCC GCAAAACCGT GCCGCCGGAG 
CCGGGCGCAG CCGTGGACTA CCAGCTGCTG ACACAGCAGG TCATCCAGTG CGCCTACGAC 
ATCGCCAAGG CGGCCAAGCA GTTGGTCACC ATCACCACCC GCGAGAAGAA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015151254.1
CDS	100..2376
Translation	MSRKAQRAEVCADCSAADPGWASINRGVLICDECCSVHRSLGRHISIVKHLRHSPWPATLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKVHPTKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQILQAELLVVYGADPGAPDVNGRTPIDYARQAAQHELAERLVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADSLDLSELAKAAKKKLQALSNRLFEELAMDVYDEVDRRENDAVWLTTQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLLIDILGEAKRRQQGKSPLSPTDALDYSLRSQSDLDDQHDYDSVASDEDTDQELLRNASRNNRARSMDSSDLSDGPITLQEYLEVKKALAASEAKVQQLMKVNNSLSDELRRLQREIHKLQSENTQIRQQAVPPHPTPAPSERPEHGHPPGTAPLHRRDRQAFSMYEPGSVLKPFGQPVEELVTRLQPFGGGEVEDEALYSMHIPASVYRMRKGPSVSSMPFPPSSPLLSCPPDGARHMSKLDRHGSGTDSDYDNTQTGEILLGVEGKRFVELSKDEDFPHELDPLDGELDPGLPSTEDVILKTEQVTKNIQELLRAAQESKHDSFVPCSEKIHSAVTEMASLFPKKPALETVRSSLRLLNASAYRLQSECRKTVPPEPGAAVDYQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_015295768.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015295768.2

ORF Insert Sequence:

ATGTCCCGGA AGGCGCAGCG GGCGGAGGTG TGCGCGGACT GCAGTGCCGC AGACCCGGGC 
TGGGCCTCCA TCAACCGCGG TGTGCTCATC TGCGACGAGT GCTGCAGCGT GCACCGCAGC 
CTGGGCCGCC ACATCTCCAT CGTCAAGCAC CTGCGGCACA GCCCCTGGCC CGCCACGCTG 
CTGCAGATGG TGCACACCTT GGCCAGCAAT GGGGCCAACT CCATCTGGGA GCATTCGCTG 
CTGGACCCGG CGCAGGTGCA GAGCGGGCGC AGGAAGGCGA ACCCCCAGGA CAAAGTGCAC 
CCCACCAAGT CGGAGTTCAT CCGCGCCAAG TACCAGATGC TGGCCTTCGT GCACAAGCTG 
CCGTGCCGGG ATGACGACGG GGTCACCGCC AAGGACCTCA GCAAGCAATT GCACTCCAGC 
GTGCGGACGG GCAACCTGGA GACGTGCCTG CGCCTGCTCT CGCTGGGGGC TCAGGCCAAC 
TTCTTCCACC CGGAGAAGGG CACCACGCCG CTGCACGTGG CTGCCAAGGC CGGGCAGATC 
CTGCAGGCAG AGCTGCTGGT GGTGTACGGC GCTGACCCCG GTGCACCCGA CGTCAACGGG 
CGGACACCCA TCGACTACGC CAGGCAGGCG GCCCAGCACG AGCTGGCGGA GCGGCTGGTG 
GAGTGCCAGT ATGAGCTGAC CGACCGGCTG GCCTTCTACC TGTGCGGCAG GAAGCCGGAC 
CACAAGAACG GGCACTACAT CATCCCGCAG ATGGCAGACA GCCTGGACCT GTCCGAGCTG 
GCCAAGGCGG CCAAGAAGAA GCTGCAAGCG CTCAGCAACC GCCTGTTTGA GGAGCTGGCC 
ATGGATGTGT ACGATGAGGT GGATCGGCGC GAGAACGACG CGGTGTGGCT GACGACGCAG 
AACCACAGCA CGTTGGTGAC CGAGCGCAGC GCCGTCCCCT TCCTCCCCGT CAACCCCGAG 
TACTCCGCCA CCCGCAACCA GGGCCGGCAG AAGTTGGCCA GGTTCAACGC CCGGGAGTTT 
GCCACTCTGC TCATCGATAT CCTGGGGGAA GCCAAGCGCC GGCAGCAGGG GAAGAGCCCG 
CTCAGCCCCA CGGATGCCCT CGACTATTCG CTGAGGAGCC AGAGTGACCT GGACGACCAG 
CACGACTACG ACAGCGTCGC CTCCGACGAG GACACGGACC AGGAGCTGCT GCGCAACGCG 
TCCCGCAACA ACCGCGCACG GAGCATGGAC TCCTCTGACC TGTCGGACGG CCCCATCACG 
CTGCAGGAGT ACCTGGAGGT GAAGAAAGCC CTGGCCGCCT CCGAGGCCAA AGTGCAGCAG 
CTGATGAAGG TGAACAACAG CCTGAGCGAT GAGCTGCGCC GGCTGCAGCG CGAGATCCAC 
AAGCTGCAAT CAGAGAACAC GCAGATCCGG CAGCAGGCGG TGCCCCCACA CCCCACCCCA 
GCCCCCAGCG AGCGCCCCGA GCACGGGCAC CCCCCCGGCA CGGCCCCCCT GCACCGCCGT 
GACCGCCAGG CCTTCTCCAT GTACGAGCCG GGCTCGGTGC TGAAACCCTT TGGGCAGCCG 
GTGGAGGAGC TGGTGACCCG GCTGCAGCCC TTCGGAGGCG GCGAGGTGGA GGACGAAGCT 
CTGTACTCCA TGCACATCCC GGCCAGCGTG TACCGGATGC GGAAGGGTCC GTCGGTCTCC 
TCCATGCCTT TTCCCCCATC CTCCCCGCTG CTCTCCTGCC CACCCGATGG TGCCCGGCAC 
ATGAGCAAAC TGGACCGGCA CGGCAGCGGC ACCGACAGCG ACTACGATAA CACGCAGACG 
GGCGAGATCC TGCTGGGCGT GGAGGGGAAG CGCTTCGTGG AGCTGAGCAA GGACGAGGAC 
TTCCCCCACG AGCTGGACCC GCTGGACGGG GAGCTGGACC CCGGGCTGCC CAGCACGGAG 
GATGTCATCC TGAAAACCGA GCAGGTCACC AAAAACATCC AGGAGCTGCT GCGGGCTGCG 
CAGGAGTCCA AACACGACAG CTTCGTGCCG TGCTCCGAGA AGATCCACTC AGCCGTGACG 
GAGATGGCGT CGCTCTTCCC CAAGAAACCG GCGCTGGAGA CGGTGCGGAG CTCCCTGCGC 
CTGCTCAATG CCAGCGCCTA CCGGCTGCAG AGCGAGTGCC GCAAAACCGT GCCGCCGGAG 
CCGGGCGCAG CCGTGGACTA CCAGCTGCTG ACACAGCAGG TCATCCAGTG CGCCTACGAC 
ATCGCCAAGG CGGCCAAGCA GTTGGTCACC ATCACCACCC GCGAGAAGAA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT1 ( XM_015295769.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015151255.1 Gallus gallus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa44317
Clone ID Related Accession (Same CDS sequence)	XM_015295769.1 , XM_015295769.2
Accession Version	XM_015295769.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ARF GTPase-activating protein GIT1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455988.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCGGA AGGCGCAGCG GGCGGAGGTG TGCGCGGACT GCAGTGCCGC AGACCCGGGC 
TGGGCCTCCA TCAACCGCGG TGTGCTCATC TGCGACGAGT GCTGCAGCGT GCACCGCAGC 
CTGGGCCGCC ACATCTCCAT CGTCAAGCAC CTGCGGCACA GCCCCTGGCC CGCCACGCTG 
CTGCAGATGG TGCACACCTT GGCCAGCAAT GGGGCCAACT CCATCTGGGA GCATTCGCTG 
CTGGACCCGG CGCAGGTGCA GAGCGGGCGC AGGAAGGCGA ACCCCCAGGA CAAAGTGCAC 
CCCACCAAGT CGGAGTTCAT CCGCGCCAAG TACCAGATGC TGGCCTTCGT GCACAAGCTG 
CCGTGCCGGG ATGACGACGG GGTCACCGCC AAGGACCTCA GCAAGCAATT GCACTCCAGC 
GTGCGGACGG GCAACCTGGA GACGTGCCTG CGCCTGCTCT CGCTGGGGGC TCAGGCCAAC 
TTCTTCCACC CGGAGAAGGG CACCACGCCG CTGCACGTGG CTGCCAAGGC CGGGCAGATC 
CTGCAGGCAG AGCTGCTGGT GGTGTACGGC GCTGACCCCG GTGCACCCGA CGTCAACGGG 
CGGACACCCA TCGACTACGC CAGGCAGGCG GCCCAGCACG AGCTGGCGGA GCGGCTGGTG 
GAGTGCCAGT ATGAGCTGAC CGACCGGCTG GCCTTCTACC TGTGCGGCAG GAAGCCGGAC 
CACAAGAACG GGCACTACAT CATCCCGCAG ATGGCAGACA GGGTGCGCCC CAAGTGCATG 
GCACAGAGCC TGGACCTGTC CGAGCTGGCC AAGGCGGCCA AGAAGAAGCT GCAAGCGCTC 
AGCAACCGCC TGTTTGAGGA GCTGGCCATG GATGTGTACG ATGAGGTGGA TCGGCGCGAG 
AACGACGCGG TGTGGCTGAC GACGCAGAAC CACAGCACGT TGGTGACCGA GCGCAGCGCC 
GTCCCCTTCC TCCCCGTCAA CCCCGAGTAC TCCGCCACCC GCAACCAGGG CCGGCAGAAG 
TTGGCCAGGT TCAACGCCCG GGAGTTTGCC ACTCTGCTCA TCGATATCCT GGGGGAAGCC 
AAGCGCCGGC AGCAGGGGAA GAGCCCGCTC AGCCCCACGG ATGCCCTCGA CTATTCGCTG 
AGGAGCCAGA GTGACCTGGA CGACCAGCAC GACTACGACA GCGTCGCCTC CGACGAGGAC 
ACGGACCAGG AGCTGCTGCG CAACGCGTCC CGCAACAACC GCGCACGGAG CATGGACTCC 
TCTGACCTGT CGGACGGCCC CATCACGCTG CAGGAGTACC TGGAGGTGAA GAAAGCCCTG 
GCCGCCTCCG AGGCCAAAGT GCAGCAGCTG ATGAAGGTGA ACAACAGCCT GAGCGATGAG 
CTGCGCCGGC TGCAGCGCGA GATCCACAAG CTGCAATCAG AGAACACGCA GATCCGGCAG 
CAGGCGGTGC CCCCACACCC CACCCCAGCC CCCAGCGAGC GCCCCGAGCA CGGGCACCCC 
CCCGGCACGG CCCCCCTGCA CCGCCGTGAC CGCCAGGCCT TCTCCATGTA CGAGCCGGGC 
TCGGTGCTGA AACCCTTTGG GCAGCCGGTG GAGGAGCTGG TGACCCGGCT GCAGCCCTTC 
GGAGGCGGCA TGCGGAAGGG TCCGTCGGTC TCCTCCATGC CTTTTCCCCC ATCCTCCCCG 
CTGCTCTCCT GCCCACCCGA TGGTGCCCGG CACATGAGCA AACTGGACCG GCACGGCAGC 
GGCACCGACA GCGACTACGA TAACACGCAG ACGGGCGAGA TCCTGCTGGG CGTGGAGGGG 
AAGCGCTTCG TGGAGCTGAG CAAGGACGAG GACTTCCCCC ACGAGCTGGA CCCGCTGGAC 
GGGGAGCTGG ACCCCGGGCT GCCCAGCACG GAGGATGTCA TCCTGAAAAC CGAGCAGGTC 
ACCAAAAACA TCCAGGAGCT GCTGCGGGCT GCGCAGGAGT CCAAACACGA CAGCTTCGTG 
CCGTGCTCCG AGAAGATCCA CTCAGCCGTG ACGGAGATGG CGTCGCTCTT CCCCAAGAAA 
CCGGCGCTGG AGACGGTGCG GAGCTCCCTG CGCCTGCTCA ATGCCAGCGC CTACCGGCTG 
CAGAGCGAGT GCCGCAAAAC CGTGCCGCCG GAGCCGGGCG CAGCCGTGGA CTACCAGCTG 
CTGACACAGC AGGTCATCCA GTGCGCCTAC GACATCGCCA AGGCGGCCAA GCAGTTGGTC 
ACCATCACCA CCCGCGAGAA GAAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015151255.1
CDS	78..2327
Translation	MSRKAQRAEVCADCSAADPGWASINRGVLICDECCSVHRSLGRHISIVKHLRHSPWPATLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKVHPTKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQILQAELLVVYGADPGAPDVNGRTPIDYARQAAQHELAERLVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADRVRPKCMAQSLDLSELAKAAKKKLQALSNRLFEELAMDVYDEVDRRENDAVWLTTQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLLIDILGEAKRRQQGKSPLSPTDALDYSLRSQSDLDDQHDYDSVASDEDTDQELLRNASRNNRARSMDSSDLSDGPITLQEYLEVKKALAASEAKVQQLMKVNNSLSDELRRLQREIHKLQSENTQIRQQAVPPHPTPAPSERPEHGHPPGTAPLHRRDRQAFSMYEPGSVLKPFGQPVEELVTRLQPFGGGMRKGPSVSSMPFPPSSPLLSCPPDGARHMSKLDRHGSGTDSDYDNTQTGEILLGVEGKRFVELSKDEDFPHELDPLDGELDPGLPSTEDVILKTEQVTKNIQELLRAAQESKHDSFVPCSEKIHSAVTEMASLFPKKPALETVRSSLRLLNASAYRLQSECRKTVPPEPGAAVDYQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_015295769.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015295769.1

ORF Insert Sequence:

ATGTCCCGGA AGGCGCAGCG GGCGGAGGTG TGCGCGGACT GCAGTGCCGC AGACCCGGGC 
TGGGCCTCCA TCAACCGCGG TGTGCTCATC TGCGACGAGT GCTGCAGCGT GCACCGCAGC 
CTGGGCCGCC ACATCTCCAT CGTCAAGCAC CTGCGGCACA GCCCCTGGCC CGCCACGCTG 
CTGCAGATGG TGCACACCTT GGCCAGCAAT GGGGCCAACT CCATCTGGGA GCATTCGCTG 
CTGGACCCGG CGCAGGTGCA GAGCGGGCGC AGGAAGGCGA ACCCCCAGGA CAAAGTGCAC 
CCCACCAAGT CGGAGTTCAT CCGCGCCAAG TACCAGATGC TGGCCTTCGT GCACAAGCTG 
CCGTGCCGGG ATGACGACGG GGTCACCGCC AAGGACCTCA GCAAGCAATT GCACTCCAGC 
GTGCGGACGG GCAACCTGGA GACGTGCCTG CGCCTGCTCT CGCTGGGGGC TCAGGCCAAC 
TTCTTCCACC CGGAGAAGGG CACCACGCCG CTGCACGTGG CTGCCAAGGC CGGGCAGATC 
CTGCAGGCAG AGCTGCTGGT GGTGTACGGC GCTGACCCCG GTGCACCCGA CGTCAACGGG 
CGGACACCCA TCGACTACGC CAGGCAGGCG GCCCAGCACG AGCTGGCGGA GCGGCTGGTG 
GAGTGCCAGT ATGAGCTGAC CGACCGGCTG GCCTTCTACC TGTGCGGCAG GAAGCCGGAC 
CACAAGAACG GGCACTACAT CATCCCGCAG ATGGCAGACA GGGTGCGCCC CAAGTGCATG 
GCACAGAGCC TGGACCTGTC CGAGCTGGCC AAGGCGGCCA AGAAGAAGCT GCAAGCGCTC 
AGCAACCGCC TGTTTGAGGA GCTGGCCATG GATGTGTACG ATGAGGTGGA TCGGCGCGAG 
AACGACGCGG TGTGGCTGAC GACGCAGAAC CACAGCACGT TGGTGACCGA GCGCAGCGCC 
GTCCCCTTCC TCCCCGTCAA CCCCGAGTAC TCCGCCACCC GCAACCAGGG CCGGCAGAAG 
TTGGCCAGGT TCAACGCCCG GGAGTTTGCC ACTCTGCTCA TCGATATCCT GGGGGAAGCC 
AAGCGCCGGC AGCAGGGGAA GAGCCCGCTC AGCCCCACGG ATGCCCTCGA CTATTCGCTG 
AGGAGCCAGA GTGACCTGGA CGACCAGCAC GACTACGACA GCGTCGCCTC CGACGAGGAC 
ACGGACCAGG AGCTGCTGCG CAACGCGTCC CGCAACAACC GCGCACGGAG CATGGACTCC 
TCTGACCTGT CGGACGGCCC CATCACGCTG CAGGAGTACC TGGAGGTGAA GAAAGCCCTG 
GCCGCCTCCG AGGCCAAAGT GCAGCAGCTG ATGAAGGTGA ACAACAGCCT GAGCGATGAG 
CTGCGCCGGC TGCAGCGCGA GATCCACAAG CTGCAATCAG AGAACACGCA GATCCGGCAG 
CAGGCGGTGC CCCCACACCC CACCCCAGCC CCCAGCGAGC GCCCCGAGCA CGGGCACCCC 
CCCGGCACGG CCCCCCTGCA CCGCCGTGAC CGCCAGGCCT TCTCCATGTA CGAGCCGGGC 
TCGGTGCTGA AACCCTTTGG GCAGCCGGTG GAGGAGCTGG TGACCCGGCT GCAGCCCTTC 
GGAGGCGGCA TGCGGAAGGG TCCGTCGGTC TCCTCCATGC CTTTTCCCCC ATCCTCCCCG 
CTGCTCTCCT GCCCACCCGA TGGTGCCCGG CACATGAGCA AACTGGACCG GCACGGCAGC 
GGCACCGACA GCGACTACGA TAACACGCAG ACGGGCGAGA TCCTGCTGGG CGTGGAGGGG 
AAGCGCTTCG TGGAGCTGAG CAAGGACGAG GACTTCCCCC ACGAGCTGGA CCCGCTGGAC 
GGGGAGCTGG ACCCCGGGCT GCCCAGCACG GAGGATGTCA TCCTGAAAAC CGAGCAGGTC 
ACCAAAAACA TCCAGGAGCT GCTGCGGGCT GCGCAGGAGT CCAAACACGA CAGCTTCGTG 
CCGTGCTCCG AGAAGATCCA CTCAGCCGTG ACGGAGATGG CGTCGCTCTT CCCCAAGAAA 
CCGGCGCTGG AGACGGTGCG GAGCTCCCTG CGCCTGCTCA ATGCCAGCGC CTACCGGCTG 
CAGAGCGAGT GCCGCAAAAC CGTGCCGCCG GAGCCGGGCG CAGCCGTGGA CTACCAGCTG 
CTGACACAGC AGGTCATCCA GTGCGCCTAC GACATCGCCA AGGCGGCCAA GCAGTTGGTC 
ACCATCACCA CCCGCGAGAA GAAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT1 ( XM_015295769.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015151255.1 Gallus gallus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa44317
Clone ID Related Accession (Same CDS sequence)	XM_015295769.1 , XM_015295769.2
Accession Version	XM_015295769.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ARF GTPase-activating protein GIT1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006106.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015295769.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCGGA AGGCGCAGCG GGCGGAGGTG TGCGCGGACT GCAGTGCCGC AGACCCGGGC 
TGGGCCTCCA TCAACCGCGG TGTGCTCATC TGCGACGAGT GCTGCAGCGT GCACCGCAGC 
CTGGGCCGCC ACATCTCCAT CGTCAAGCAC CTGCGGCACA GCCCCTGGCC CGCCACGCTG 
CTGCAGATGG TGCACACCTT GGCCAGCAAT GGGGCCAACT CCATCTGGGA GCATTCGCTG 
CTGGACCCGG CGCAGGTGCA GAGCGGGCGC AGGAAGGCGA ACCCCCAGGA CAAAGTGCAC 
CCCACCAAGT CGGAGTTCAT CCGCGCCAAG TACCAGATGC TGGCCTTCGT GCACAAGCTG 
CCGTGCCGGG ATGACGACGG GGTCACCGCC AAGGACCTCA GCAAGCAATT GCACTCCAGC 
GTGCGGACGG GCAACCTGGA GACGTGCCTG CGCCTGCTCT CGCTGGGGGC TCAGGCCAAC 
TTCTTCCACC CGGAGAAGGG CACCACGCCG CTGCACGTGG CTGCCAAGGC CGGGCAGATC 
CTGCAGGCAG AGCTGCTGGT GGTGTACGGC GCTGACCCCG GTGCACCCGA CGTCAACGGG 
CGGACACCCA TCGACTACGC CAGGCAGGCG GCCCAGCACG AGCTGGCGGA GCGGCTGGTG 
GAGTGCCAGT ATGAGCTGAC CGACCGGCTG GCCTTCTACC TGTGCGGCAG GAAGCCGGAC 
CACAAGAACG GGCACTACAT CATCCCGCAG ATGGCAGACA GGGTGCGCCC CAAGTGCATG 
GCACAGAGCC TGGACCTGTC CGAGCTGGCC AAGGCGGCCA AGAAGAAGCT GCAAGCGCTC 
AGCAACCGCC TGTTTGAGGA GCTGGCCATG GATGTGTACG ATGAGGTGGA TCGGCGCGAG 
AACGACGCGG TGTGGCTGAC GACGCAGAAC CACAGCACGT TGGTGACCGA GCGCAGCGCC 
GTCCCCTTCC TCCCCGTCAA CCCCGAGTAC TCCGCCACCC GCAACCAGGG CCGGCAGAAG 
TTGGCCAGGT TCAACGCCCG GGAGTTTGCC ACTCTGCTCA TCGATATCCT GGGGGAAGCC 
AAGCGCCGGC AGCAGGGGAA GAGCCCGCTC AGCCCCACGG ATGCCCTCGA CTATTCGCTG 
AGGAGCCAGA GTGACCTGGA CGACCAGCAC GACTACGACA GCGTCGCCTC CGACGAGGAC 
ACGGACCAGG AGCTGCTGCG CAACGCGTCC CGCAACAACC GCGCACGGAG CATGGACTCC 
TCTGACCTGT CGGACGGCCC CATCACGCTG CAGGAGTACC TGGAGGTGAA GAAAGCCCTG 
GCCGCCTCCG AGGCCAAAGT GCAGCAGCTG ATGAAGGTGA ACAACAGCCT GAGCGATGAG 
CTGCGCCGGC TGCAGCGCGA GATCCACAAG CTGCAATCAG AGAACACGCA GATCCGGCAG 
CAGGCGGTGC CCCCACACCC CACCCCAGCC CCCAGCGAGC GCCCCGAGCA CGGGCACCCC 
CCCGGCACGG CCCCCCTGCA CCGCCGTGAC CGCCAGGCCT TCTCCATGTA CGAGCCGGGC 
TCGGTGCTGA AACCCTTTGG GCAGCCGGTG GAGGAGCTGG TGACCCGGCT GCAGCCCTTC 
GGAGGCGGCA TGCGGAAGGG TCCGTCGGTC TCCTCCATGC CTTTTCCCCC ATCCTCCCCG 
CTGCTCTCCT GCCCACCCGA TGGTGCCCGG CACATGAGCA AACTGGACCG GCACGGCAGC 
GGCACCGACA GCGACTACGA TAACACGCAG ACGGGCGAGA TCCTGCTGGG CGTGGAGGGG 
AAGCGCTTCG TGGAGCTGAG CAAGGACGAG GACTTCCCCC ACGAGCTGGA CCCGCTGGAC 
GGGGAGCTGG ACCCCGGGCT GCCCAGCACG GAGGATGTCA TCCTGAAAAC CGAGCAGGTC 
ACCAAAAACA TCCAGGAGCT GCTGCGGGCT GCGCAGGAGT CCAAACACGA CAGCTTCGTG 
CCGTGCTCCG AGAAGATCCA CTCAGCCGTG ACGGAGATGG CGTCGCTCTT CCCCAAGAAA 
CCGGCGCTGG AGACGGTGCG GAGCTCCCTG CGCCTGCTCA ATGCCAGCGC CTACCGGCTG 
CAGAGCGAGT GCCGCAAAAC CGTGCCGCCG GAGCCGGGCG CAGCCGTGGA CTACCAGCTG 
CTGACACAGC AGGTCATCCA GTGCGCCTAC GACATCGCCA AGGCGGCCAA GCAGTTGGTC 
ACCATCACCA CCCGCGAGAA GAAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015151255.1
CDS	100..2349
Translation	MSRKAQRAEVCADCSAADPGWASINRGVLICDECCSVHRSLGRHISIVKHLRHSPWPATLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKVHPTKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQILQAELLVVYGADPGAPDVNGRTPIDYARQAAQHELAERLVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADRVRPKCMAQSLDLSELAKAAKKKLQALSNRLFEELAMDVYDEVDRRENDAVWLTTQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLLIDILGEAKRRQQGKSPLSPTDALDYSLRSQSDLDDQHDYDSVASDEDTDQELLRNASRNNRARSMDSSDLSDGPITLQEYLEVKKALAASEAKVQQLMKVNNSLSDELRRLQREIHKLQSENTQIRQQAVPPHPTPAPSERPEHGHPPGTAPLHRRDRQAFSMYEPGSVLKPFGQPVEELVTRLQPFGGGMRKGPSVSSMPFPPSSPLLSCPPDGARHMSKLDRHGSGTDSDYDNTQTGEILLGVEGKRFVELSKDEDFPHELDPLDGELDPGLPSTEDVILKTEQVTKNIQELLRAAQESKHDSFVPCSEKIHSAVTEMASLFPKKPALETVRSSLRLLNASAYRLQSECRKTVPPEPGAAVDYQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_015295769.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015295769.2

ORF Insert Sequence:

ATGTCCCGGA AGGCGCAGCG GGCGGAGGTG TGCGCGGACT GCAGTGCCGC AGACCCGGGC 
TGGGCCTCCA TCAACCGCGG TGTGCTCATC TGCGACGAGT GCTGCAGCGT GCACCGCAGC 
CTGGGCCGCC ACATCTCCAT CGTCAAGCAC CTGCGGCACA GCCCCTGGCC CGCCACGCTG 
CTGCAGATGG TGCACACCTT GGCCAGCAAT GGGGCCAACT CCATCTGGGA GCATTCGCTG 
CTGGACCCGG CGCAGGTGCA GAGCGGGCGC AGGAAGGCGA ACCCCCAGGA CAAAGTGCAC 
CCCACCAAGT CGGAGTTCAT CCGCGCCAAG TACCAGATGC TGGCCTTCGT GCACAAGCTG 
CCGTGCCGGG ATGACGACGG GGTCACCGCC AAGGACCTCA GCAAGCAATT GCACTCCAGC 
GTGCGGACGG GCAACCTGGA GACGTGCCTG CGCCTGCTCT CGCTGGGGGC TCAGGCCAAC 
TTCTTCCACC CGGAGAAGGG CACCACGCCG CTGCACGTGG CTGCCAAGGC CGGGCAGATC 
CTGCAGGCAG AGCTGCTGGT GGTGTACGGC GCTGACCCCG GTGCACCCGA CGTCAACGGG 
CGGACACCCA TCGACTACGC CAGGCAGGCG GCCCAGCACG AGCTGGCGGA GCGGCTGGTG 
GAGTGCCAGT ATGAGCTGAC CGACCGGCTG GCCTTCTACC TGTGCGGCAG GAAGCCGGAC 
CACAAGAACG GGCACTACAT CATCCCGCAG ATGGCAGACA GGGTGCGCCC CAAGTGCATG 
GCACAGAGCC TGGACCTGTC CGAGCTGGCC AAGGCGGCCA AGAAGAAGCT GCAAGCGCTC 
AGCAACCGCC TGTTTGAGGA GCTGGCCATG GATGTGTACG ATGAGGTGGA TCGGCGCGAG 
AACGACGCGG TGTGGCTGAC GACGCAGAAC CACAGCACGT TGGTGACCGA GCGCAGCGCC 
GTCCCCTTCC TCCCCGTCAA CCCCGAGTAC TCCGCCACCC GCAACCAGGG CCGGCAGAAG 
TTGGCCAGGT TCAACGCCCG GGAGTTTGCC ACTCTGCTCA TCGATATCCT GGGGGAAGCC 
AAGCGCCGGC AGCAGGGGAA GAGCCCGCTC AGCCCCACGG ATGCCCTCGA CTATTCGCTG 
AGGAGCCAGA GTGACCTGGA CGACCAGCAC GACTACGACA GCGTCGCCTC CGACGAGGAC 
ACGGACCAGG AGCTGCTGCG CAACGCGTCC CGCAACAACC GCGCACGGAG CATGGACTCC 
TCTGACCTGT CGGACGGCCC CATCACGCTG CAGGAGTACC TGGAGGTGAA GAAAGCCCTG 
GCCGCCTCCG AGGCCAAAGT GCAGCAGCTG ATGAAGGTGA ACAACAGCCT GAGCGATGAG 
CTGCGCCGGC TGCAGCGCGA GATCCACAAG CTGCAATCAG AGAACACGCA GATCCGGCAG 
CAGGCGGTGC CCCCACACCC CACCCCAGCC CCCAGCGAGC GCCCCGAGCA CGGGCACCCC 
CCCGGCACGG CCCCCCTGCA CCGCCGTGAC CGCCAGGCCT TCTCCATGTA CGAGCCGGGC 
TCGGTGCTGA AACCCTTTGG GCAGCCGGTG GAGGAGCTGG TGACCCGGCT GCAGCCCTTC 
GGAGGCGGCA TGCGGAAGGG TCCGTCGGTC TCCTCCATGC CTTTTCCCCC ATCCTCCCCG 
CTGCTCTCCT GCCCACCCGA TGGTGCCCGG CACATGAGCA AACTGGACCG GCACGGCAGC 
GGCACCGACA GCGACTACGA TAACACGCAG ACGGGCGAGA TCCTGCTGGG CGTGGAGGGG 
AAGCGCTTCG TGGAGCTGAG CAAGGACGAG GACTTCCCCC ACGAGCTGGA CCCGCTGGAC 
GGGGAGCTGG ACCCCGGGCT GCCCAGCACG GAGGATGTCA TCCTGAAAAC CGAGCAGGTC 
ACCAAAAACA TCCAGGAGCT GCTGCGGGCT GCGCAGGAGT CCAAACACGA CAGCTTCGTG 
CCGTGCTCCG AGAAGATCCA CTCAGCCGTG ACGGAGATGG CGTCGCTCTT CCCCAAGAAA 
CCGGCGCTGG AGACGGTGCG GAGCTCCCTG CGCCTGCTCA ATGCCAGCGC CTACCGGCTG 
CAGAGCGAGT GCCGCAAAAC CGTGCCGCCG GAGCCGGGCG CAGCCGTGGA CTACCAGCTG 
CTGACACAGC AGGTCATCCA GTGCGCCTAC GACATCGCCA AGGCGGCCAA GCAGTTGGTC 
ACCATCACCA CCCGCGAGAA GAAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT1 ( XM_015295770.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015151256.1 Gallus gallus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa44318
Clone ID Related Accession (Same CDS sequence)	XM_015295770.1 , XM_015295770.2
Accession Version	XM_015295770.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ARF GTPase-activating protein GIT1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455988.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCGGA AGGCGCAGCG GGCGGAGGTG TGCGCGGACT GCAGTGCCGC AGACCCGGGC 
TGGGCCTCCA TCAACCGCGG TGTGCTCATC TGCGACGAGT GCTGCAGCGT GCACCGCAGC 
CTGGGCCGCC ACATCTCCAT CGTCAAGCAC CTGCGGCACA GCCCCTGGCC CGCCACGCTG 
CTGCAGATGG TGCACACCTT GGCCAGCAAT GGGGCCAACT CCATCTGGGA GCATTCGCTG 
CTGGACCCGG CGCAGGTGCA GAGCGGGCGC AGGAAGGCGA ACCCCCAGGA CAAAGTGCAC 
CCCACCAAGT CGGAGTTCAT CCGCGCCAAG TACCAGATGC TGGCCTTCGT GCACAAGCTG 
CCGTGCCGGG ATGACGACGG GGTCACCGCC AAGGACCTCA GCAAGCAATT GCACTCCAGC 
GTGCGGACGG GCAACCTGGA GACGTGCCTG CGCCTGCTCT CGCTGGGGGC TCAGGCCAAC 
TTCTTCCACC CGGAGAAGGG CACCACGCCG CTGCACGTGG CTGCCAAGGC CGGGCAGATC 
CTGCAGGCAG AGCTGCTGGT GGTGTACGGC GCTGACCCCG GTGCACCCGA CGTCAACGGG 
CGGACACCCA TCGACTACGC CAGGCAGGCG GCCCAGCACG AGCTGGCGGA GCGGCTGGTG 
GAGTGCCAGT ATGAGCTGAC CGACCGGCTG GCCTTCTACC TGTGCGGCAG GAAGCCGGAC 
CACAAGAACG GGCACTACAT CATCCCGCAG ATGGCAGACA GGGTGCGCCC CAAGTGCATG 
GCACAGAGCC TGGACCTGTC CGAGCTGGCC AAGGCGGCCA AGAAGAAGCT GCAAGCGCTC 
AGCAACCGCC TGTTTGAGGA GCTGGCCATG GATGTGTACG ATGAGGTGGA TCGGCGCGAG 
AACGACGCGG TGTGGCTGAC GACGCAGAAC CACAGCACGT TGGTGACCGA GCGCAGCGCC 
GTCCCCTTCC TCCCCGTCAA CCCCGAGTAC TCCGCCACCC GCAACCAGGG CCGGCAGAAG 
TTGGCCAGGT TCAACGCCCG GGAGTTTGCC ACTCTGCTCA TCGATATCCT GGGGGAAGCC 
AAGCGCCGGC AGCAGGGGAA GAGCCCGCTC AGCCCCACGG ATGCCCTCGA CTATTCGCTG 
AGGAGCCAGA GTGACCTGGA CGACCAGCAC GACTACGACA GCGTCGCCTC CGACGAGGAC 
ACGGACCAGG AGCTGCTGCG CAACGCGTCC CGCAACAACC GCGCACGGAG CATGGACTCC 
TCTGACCTGT CGGACGGCCC CATCACGCTG CAGGAGTACC TGGAGGTGAA GAAAGCCCTG 
GCCGCCTCCG AGGCCAAAGT GCAGCAGCTG ATGAAGGTGA ACAACAGCCT GAGCGATGAG 
CTGCGCCGGC TGCAGCGCGA GATCCACAAG CTGCAATCAG AGAACACGCA GATCCGGCAG 
CAGGCGGTGC CCCCACACCC CACCCCAGCC CCCAGCGAGC GCCCCGAGCA CGGGCACCCC 
CCCGGCACGG CCCCCCTGCA CCGCCGTGAC CGCCAGGCCT TCTCCATGTA CGAGCCGGGC 
TCGGTGCTGA AACCCTTTGG GCAGCCGGTG GAGGAGCTGG TGACCCGGCT GCAGCCCTTC 
GGAGGCGGCG AGGTGGAGGA CGAAGCTCTG TACTCCATGC ACATCCCGGC CAGCGTGTAC 
CGGATGCGGA AGGGTCCGTC GGTCTCCTCC ATGCCTTTTC CCCCATCCTC CCCGCTGCTC 
TCCTGCCCAC CCGATGGTGC CCGGCACATG AGCAAACTGG ACCGGCACGG CAGCGGCACC 
GACAGCGACT ACGATAACAC GCAGACGGGC GAGATCCTGC TGGGCGTGGA GGGGAAGCGC 
TTCGTGGAGC TGAGCAAGGA CGAGGACTTC CCCCACGAGC TGGACCCGCT GGACGGGGAG 
CTGGACCCCG GGCTGCCCAG CACGGAGGAT GTCATCCTGA AAACCGAGCA GGTCACCAAA 
AACATCCAGG AGCTGCTGCG GGCTGCGCAG GAGTCCAAAC ACGACAGCTT CGTGCCGTGC 
TCCGAGAAGA TCCACTCAGC CGTGACGGAG ATGGCGTCGC TCTTCCCCAA GAAACCGGCG 
CTGGAGACGG TGCGGAGCTC CCTGCGCCTG CTCAATGCCA GCGCCTACCG GCTGCAGAGC 
GAGTGCCGCA AAACCGTGCC GCCGGAGCCG GGCGCAGCCG TGGACTACCA GCTGCTGACA 
CAGCAGGTCA TCCAGTGCGC CTACGACATC GCCAAGGCGG CCAAGCAGTT GGTCACCATC 
ACCACCCGCG AGAAGAAGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015151256.1
CDS	79..2382
Translation	MSRKAQRAEVCADCSAADPGWASINRGVLICDECCSVHRSLGRHISIVKHLRHSPWPATLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKVHPTKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQILQAELLVVYGADPGAPDVNGRTPIDYARQAAQHELAERLVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADRVRPKCMAQSLDLSELAKAAKKKLQALSNRLFEELAMDVYDEVDRRENDAVWLTTQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLLIDILGEAKRRQQGKSPLSPTDALDYSLRSQSDLDDQHDYDSVASDEDTDQELLRNASRNNRARSMDSSDLSDGPITLQEYLEVKKALAASEAKVQQLMKVNNSLSDELRRLQREIHKLQSENTQIRQQAVPPHPTPAPSERPEHGHPPGTAPLHRRDRQAFSMYEPGSVLKPFGQPVEELVTRLQPFGGGEVEDEALYSMHIPASVYRMRKGPSVSSMPFPPSSPLLSCPPDGARHMSKLDRHGSGTDSDYDNTQTGEILLGVEGKRFVELSKDEDFPHELDPLDGELDPGLPSTEDVILKTEQVTKNIQELLRAAQESKHDSFVPCSEKIHSAVTEMASLFPKKPALETVRSSLRLLNASAYRLQSECRKTVPPEPGAAVDYQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_015295770.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015295770.1

ORF Insert Sequence:

ATGTCCCGGA AGGCGCAGCG GGCGGAGGTG TGCGCGGACT GCAGTGCCGC AGACCCGGGC 
TGGGCCTCCA TCAACCGCGG TGTGCTCATC TGCGACGAGT GCTGCAGCGT GCACCGCAGC 
CTGGGCCGCC ACATCTCCAT CGTCAAGCAC CTGCGGCACA GCCCCTGGCC CGCCACGCTG 
CTGCAGATGG TGCACACCTT GGCCAGCAAT GGGGCCAACT CCATCTGGGA GCATTCGCTG 
CTGGACCCGG CGCAGGTGCA GAGCGGGCGC AGGAAGGCGA ACCCCCAGGA CAAAGTGCAC 
CCCACCAAGT CGGAGTTCAT CCGCGCCAAG TACCAGATGC TGGCCTTCGT GCACAAGCTG 
CCGTGCCGGG ATGACGACGG GGTCACCGCC AAGGACCTCA GCAAGCAATT GCACTCCAGC 
GTGCGGACGG GCAACCTGGA GACGTGCCTG CGCCTGCTCT CGCTGGGGGC TCAGGCCAAC 
TTCTTCCACC CGGAGAAGGG CACCACGCCG CTGCACGTGG CTGCCAAGGC CGGGCAGATC 
CTGCAGGCAG AGCTGCTGGT GGTGTACGGC GCTGACCCCG GTGCACCCGA CGTCAACGGG 
CGGACACCCA TCGACTACGC CAGGCAGGCG GCCCAGCACG AGCTGGCGGA GCGGCTGGTG 
GAGTGCCAGT ATGAGCTGAC CGACCGGCTG GCCTTCTACC TGTGCGGCAG GAAGCCGGAC 
CACAAGAACG GGCACTACAT CATCCCGCAG ATGGCAGACA GGGTGCGCCC CAAGTGCATG 
GCACAGAGCC TGGACCTGTC CGAGCTGGCC AAGGCGGCCA AGAAGAAGCT GCAAGCGCTC 
AGCAACCGCC TGTTTGAGGA GCTGGCCATG GATGTGTACG ATGAGGTGGA TCGGCGCGAG 
AACGACGCGG TGTGGCTGAC GACGCAGAAC CACAGCACGT TGGTGACCGA GCGCAGCGCC 
GTCCCCTTCC TCCCCGTCAA CCCCGAGTAC TCCGCCACCC GCAACCAGGG CCGGCAGAAG 
TTGGCCAGGT TCAACGCCCG GGAGTTTGCC ACTCTGCTCA TCGATATCCT GGGGGAAGCC 
AAGCGCCGGC AGCAGGGGAA GAGCCCGCTC AGCCCCACGG ATGCCCTCGA CTATTCGCTG 
AGGAGCCAGA GTGACCTGGA CGACCAGCAC GACTACGACA GCGTCGCCTC CGACGAGGAC 
ACGGACCAGG AGCTGCTGCG CAACGCGTCC CGCAACAACC GCGCACGGAG CATGGACTCC 
TCTGACCTGT CGGACGGCCC CATCACGCTG CAGGAGTACC TGGAGGTGAA GAAAGCCCTG 
GCCGCCTCCG AGGCCAAAGT GCAGCAGCTG ATGAAGGTGA ACAACAGCCT GAGCGATGAG 
CTGCGCCGGC TGCAGCGCGA GATCCACAAG CTGCAATCAG AGAACACGCA GATCCGGCAG 
CAGGCGGTGC CCCCACACCC CACCCCAGCC CCCAGCGAGC GCCCCGAGCA CGGGCACCCC 
CCCGGCACGG CCCCCCTGCA CCGCCGTGAC CGCCAGGCCT TCTCCATGTA CGAGCCGGGC 
TCGGTGCTGA AACCCTTTGG GCAGCCGGTG GAGGAGCTGG TGACCCGGCT GCAGCCCTTC 
GGAGGCGGCG AGGTGGAGGA CGAAGCTCTG TACTCCATGC ACATCCCGGC CAGCGTGTAC 
CGGATGCGGA AGGGTCCGTC GGTCTCCTCC ATGCCTTTTC CCCCATCCTC CCCGCTGCTC 
TCCTGCCCAC CCGATGGTGC CCGGCACATG AGCAAACTGG ACCGGCACGG CAGCGGCACC 
GACAGCGACT ACGATAACAC GCAGACGGGC GAGATCCTGC TGGGCGTGGA GGGGAAGCGC 
TTCGTGGAGC TGAGCAAGGA CGAGGACTTC CCCCACGAGC TGGACCCGCT GGACGGGGAG 
CTGGACCCCG GGCTGCCCAG CACGGAGGAT GTCATCCTGA AAACCGAGCA GGTCACCAAA 
AACATCCAGG AGCTGCTGCG GGCTGCGCAG GAGTCCAAAC ACGACAGCTT CGTGCCGTGC 
TCCGAGAAGA TCCACTCAGC CGTGACGGAG ATGGCGTCGC TCTTCCCCAA GAAACCGGCG 
CTGGAGACGG TGCGGAGCTC CCTGCGCCTG CTCAATGCCA GCGCCTACCG GCTGCAGAGC 
GAGTGCCGCA AAACCGTGCC GCCGGAGCCG GGCGCAGCCG TGGACTACCA GCTGCTGACA 
CAGCAGGTCA TCCAGTGCGC CTACGACATC GCCAAGGCGG CCAAGCAGTT GGTCACCATC 
ACCACCCGCG AGAAGAAGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT1 ( XM_015295770.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015151256.1 Gallus gallus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa44318
Clone ID Related Accession (Same CDS sequence)	XM_015295770.1 , XM_015295770.2
Accession Version	XM_015295770.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ARF GTPase-activating protein GIT1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006106.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015295770.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCGGA AGGCGCAGCG GGCGGAGGTG TGCGCGGACT GCAGTGCCGC AGACCCGGGC 
TGGGCCTCCA TCAACCGCGG TGTGCTCATC TGCGACGAGT GCTGCAGCGT GCACCGCAGC 
CTGGGCCGCC ACATCTCCAT CGTCAAGCAC CTGCGGCACA GCCCCTGGCC CGCCACGCTG 
CTGCAGATGG TGCACACCTT GGCCAGCAAT GGGGCCAACT CCATCTGGGA GCATTCGCTG 
CTGGACCCGG CGCAGGTGCA GAGCGGGCGC AGGAAGGCGA ACCCCCAGGA CAAAGTGCAC 
CCCACCAAGT CGGAGTTCAT CCGCGCCAAG TACCAGATGC TGGCCTTCGT GCACAAGCTG 
CCGTGCCGGG ATGACGACGG GGTCACCGCC AAGGACCTCA GCAAGCAATT GCACTCCAGC 
GTGCGGACGG GCAACCTGGA GACGTGCCTG CGCCTGCTCT CGCTGGGGGC TCAGGCCAAC 
TTCTTCCACC CGGAGAAGGG CACCACGCCG CTGCACGTGG CTGCCAAGGC CGGGCAGATC 
CTGCAGGCAG AGCTGCTGGT GGTGTACGGC GCTGACCCCG GTGCACCCGA CGTCAACGGG 
CGGACACCCA TCGACTACGC CAGGCAGGCG GCCCAGCACG AGCTGGCGGA GCGGCTGGTG 
GAGTGCCAGT ATGAGCTGAC CGACCGGCTG GCCTTCTACC TGTGCGGCAG GAAGCCGGAC 
CACAAGAACG GGCACTACAT CATCCCGCAG ATGGCAGACA GGGTGCGCCC CAAGTGCATG 
GCACAGAGCC TGGACCTGTC CGAGCTGGCC AAGGCGGCCA AGAAGAAGCT GCAAGCGCTC 
AGCAACCGCC TGTTTGAGGA GCTGGCCATG GATGTGTACG ATGAGGTGGA TCGGCGCGAG 
AACGACGCGG TGTGGCTGAC GACGCAGAAC CACAGCACGT TGGTGACCGA GCGCAGCGCC 
GTCCCCTTCC TCCCCGTCAA CCCCGAGTAC TCCGCCACCC GCAACCAGGG CCGGCAGAAG 
TTGGCCAGGT TCAACGCCCG GGAGTTTGCC ACTCTGCTCA TCGATATCCT GGGGGAAGCC 
AAGCGCCGGC AGCAGGGGAA GAGCCCGCTC AGCCCCACGG ATGCCCTCGA CTATTCGCTG 
AGGAGCCAGA GTGACCTGGA CGACCAGCAC GACTACGACA GCGTCGCCTC CGACGAGGAC 
ACGGACCAGG AGCTGCTGCG CAACGCGTCC CGCAACAACC GCGCACGGAG CATGGACTCC 
TCTGACCTGT CGGACGGCCC CATCACGCTG CAGGAGTACC TGGAGGTGAA GAAAGCCCTG 
GCCGCCTCCG AGGCCAAAGT GCAGCAGCTG ATGAAGGTGA ACAACAGCCT GAGCGATGAG 
CTGCGCCGGC TGCAGCGCGA GATCCACAAG CTGCAATCAG AGAACACGCA GATCCGGCAG 
CAGGCGGTGC CCCCACACCC CACCCCAGCC CCCAGCGAGC GCCCCGAGCA CGGGCACCCC 
CCCGGCACGG CCCCCCTGCA CCGCCGTGAC CGCCAGGCCT TCTCCATGTA CGAGCCGGGC 
TCGGTGCTGA AACCCTTTGG GCAGCCGGTG GAGGAGCTGG TGACCCGGCT GCAGCCCTTC 
GGAGGCGGCG AGGTGGAGGA CGAAGCTCTG TACTCCATGC ACATCCCGGC CAGCGTGTAC 
CGGATGCGGA AGGGTCCGTC GGTCTCCTCC ATGCCTTTTC CCCCATCCTC CCCGCTGCTC 
TCCTGCCCAC CCGATGGTGC CCGGCACATG AGCAAACTGG ACCGGCACGG CAGCGGCACC 
GACAGCGACT ACGATAACAC GCAGACGGGC GAGATCCTGC TGGGCGTGGA GGGGAAGCGC 
TTCGTGGAGC TGAGCAAGGA CGAGGACTTC CCCCACGAGC TGGACCCGCT GGACGGGGAG 
CTGGACCCCG GGCTGCCCAG CACGGAGGAT GTCATCCTGA AAACCGAGCA GGTCACCAAA 
AACATCCAGG AGCTGCTGCG GGCTGCGCAG GAGTCCAAAC ACGACAGCTT CGTGCCGTGC 
TCCGAGAAGA TCCACTCAGC CGTGACGGAG ATGGCGTCGC TCTTCCCCAA GAAACCGGCG 
CTGGAGACGG TGCGGAGCTC CCTGCGCCTG CTCAATGCCA GCGCCTACCG GCTGCAGAGC 
GAGTGCCGCA AAACCGTGCC GCCGGAGCCG GGCGCAGCCG TGGACTACCA GCTGCTGACA 
CAGCAGGTCA TCCAGTGCGC CTACGACATC GCCAAGGCGG CCAAGCAGTT GGTCACCATC 
ACCACCCGCG AGAAGAAGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015151256.1
CDS	104..2407
Translation	MSRKAQRAEVCADCSAADPGWASINRGVLICDECCSVHRSLGRHISIVKHLRHSPWPATLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKVHPTKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQILQAELLVVYGADPGAPDVNGRTPIDYARQAAQHELAERLVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADRVRPKCMAQSLDLSELAKAAKKKLQALSNRLFEELAMDVYDEVDRRENDAVWLTTQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLLIDILGEAKRRQQGKSPLSPTDALDYSLRSQSDLDDQHDYDSVASDEDTDQELLRNASRNNRARSMDSSDLSDGPITLQEYLEVKKALAASEAKVQQLMKVNNSLSDELRRLQREIHKLQSENTQIRQQAVPPHPTPAPSERPEHGHPPGTAPLHRRDRQAFSMYEPGSVLKPFGQPVEELVTRLQPFGGGEVEDEALYSMHIPASVYRMRKGPSVSSMPFPPSSPLLSCPPDGARHMSKLDRHGSGTDSDYDNTQTGEILLGVEGKRFVELSKDEDFPHELDPLDGELDPGLPSTEDVILKTEQVTKNIQELLRAAQESKHDSFVPCSEKIHSAVTEMASLFPKKPALETVRSSLRLLNASAYRLQSECRKTVPPEPGAAVDYQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_015295770.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015295770.2

ORF Insert Sequence:

ATGTCCCGGA AGGCGCAGCG GGCGGAGGTG TGCGCGGACT GCAGTGCCGC AGACCCGGGC 
TGGGCCTCCA TCAACCGCGG TGTGCTCATC TGCGACGAGT GCTGCAGCGT GCACCGCAGC 
CTGGGCCGCC ACATCTCCAT CGTCAAGCAC CTGCGGCACA GCCCCTGGCC CGCCACGCTG 
CTGCAGATGG TGCACACCTT GGCCAGCAAT GGGGCCAACT CCATCTGGGA GCATTCGCTG 
CTGGACCCGG CGCAGGTGCA GAGCGGGCGC AGGAAGGCGA ACCCCCAGGA CAAAGTGCAC 
CCCACCAAGT CGGAGTTCAT CCGCGCCAAG TACCAGATGC TGGCCTTCGT GCACAAGCTG 
CCGTGCCGGG ATGACGACGG GGTCACCGCC AAGGACCTCA GCAAGCAATT GCACTCCAGC 
GTGCGGACGG GCAACCTGGA GACGTGCCTG CGCCTGCTCT CGCTGGGGGC TCAGGCCAAC 
TTCTTCCACC CGGAGAAGGG CACCACGCCG CTGCACGTGG CTGCCAAGGC CGGGCAGATC 
CTGCAGGCAG AGCTGCTGGT GGTGTACGGC GCTGACCCCG GTGCACCCGA CGTCAACGGG 
CGGACACCCA TCGACTACGC CAGGCAGGCG GCCCAGCACG AGCTGGCGGA GCGGCTGGTG 
GAGTGCCAGT ATGAGCTGAC CGACCGGCTG GCCTTCTACC TGTGCGGCAG GAAGCCGGAC 
CACAAGAACG GGCACTACAT CATCCCGCAG ATGGCAGACA GGGTGCGCCC CAAGTGCATG 
GCACAGAGCC TGGACCTGTC CGAGCTGGCC AAGGCGGCCA AGAAGAAGCT GCAAGCGCTC 
AGCAACCGCC TGTTTGAGGA GCTGGCCATG GATGTGTACG ATGAGGTGGA TCGGCGCGAG 
AACGACGCGG TGTGGCTGAC GACGCAGAAC CACAGCACGT TGGTGACCGA GCGCAGCGCC 
GTCCCCTTCC TCCCCGTCAA CCCCGAGTAC TCCGCCACCC GCAACCAGGG CCGGCAGAAG 
TTGGCCAGGT TCAACGCCCG GGAGTTTGCC ACTCTGCTCA TCGATATCCT GGGGGAAGCC 
AAGCGCCGGC AGCAGGGGAA GAGCCCGCTC AGCCCCACGG ATGCCCTCGA CTATTCGCTG 
AGGAGCCAGA GTGACCTGGA CGACCAGCAC GACTACGACA GCGTCGCCTC CGACGAGGAC 
ACGGACCAGG AGCTGCTGCG CAACGCGTCC CGCAACAACC GCGCACGGAG CATGGACTCC 
TCTGACCTGT CGGACGGCCC CATCACGCTG CAGGAGTACC TGGAGGTGAA GAAAGCCCTG 
GCCGCCTCCG AGGCCAAAGT GCAGCAGCTG ATGAAGGTGA ACAACAGCCT GAGCGATGAG 
CTGCGCCGGC TGCAGCGCGA GATCCACAAG CTGCAATCAG AGAACACGCA GATCCGGCAG 
CAGGCGGTGC CCCCACACCC CACCCCAGCC CCCAGCGAGC GCCCCGAGCA CGGGCACCCC 
CCCGGCACGG CCCCCCTGCA CCGCCGTGAC CGCCAGGCCT TCTCCATGTA CGAGCCGGGC 
TCGGTGCTGA AACCCTTTGG GCAGCCGGTG GAGGAGCTGG TGACCCGGCT GCAGCCCTTC 
GGAGGCGGCG AGGTGGAGGA CGAAGCTCTG TACTCCATGC ACATCCCGGC CAGCGTGTAC 
CGGATGCGGA AGGGTCCGTC GGTCTCCTCC ATGCCTTTTC CCCCATCCTC CCCGCTGCTC 
TCCTGCCCAC CCGATGGTGC CCGGCACATG AGCAAACTGG ACCGGCACGG CAGCGGCACC 
GACAGCGACT ACGATAACAC GCAGACGGGC GAGATCCTGC TGGGCGTGGA GGGGAAGCGC 
TTCGTGGAGC TGAGCAAGGA CGAGGACTTC CCCCACGAGC TGGACCCGCT GGACGGGGAG 
CTGGACCCCG GGCTGCCCAG CACGGAGGAT GTCATCCTGA AAACCGAGCA GGTCACCAAA 
AACATCCAGG AGCTGCTGCG GGCTGCGCAG GAGTCCAAAC ACGACAGCTT CGTGCCGTGC 
TCCGAGAAGA TCCACTCAGC CGTGACGGAG ATGGCGTCGC TCTTCCCCAA GAAACCGGCG 
CTGGAGACGG TGCGGAGCTC CCTGCGCCTG CTCAATGCCA GCGCCTACCG GCTGCAGAGC 
GAGTGCCGCA AAACCGTGCC GCCGGAGCCG GGCGCAGCCG TGGACTACCA GCTGCTGACA 
CAGCAGGTCA TCCAGTGCGC CTACGACATC GCCAAGGCGG CCAAGCAGTT GGTCACCATC 
ACCACCCGCG AGAAGAAGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of the endogenous GIT1-betaPIX complex, and identification of its association to membranes.
European journal of cell biology85(1)35-46(2006 Jan)
Botrugno OA,Paris S,Za L,Gualdoni S,Cattaneo A,Bachi A,de Curtis I

p95-APP1 links membrane transport to Rac-mediated reorganization of actin.
Nature cell biology2(8)521-30(2000 Aug)
Di Cesare A,Paris S,Albertinazzi C,Dariozzi S,Andersen J,Mann M,Longhi R,de Curtis I

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GIT1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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