PRKCA cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRKCA
Entrez Gene ID	417430
Full Name	protein kinase C alpha
General protein information	Preferred Names protein kinase C alpha Names protein kinase C alpha type
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004946229.2	XP_004946286.1	protein kinase C alpha type isoform X1
XM_004946229.3	XP_004946286.1	protein kinase C alpha type isoform X1
XM_025141605.1	XP_024997373.1	protein kinase C alpha type isoform X2
NM_001012804.1	NP_001012822.1	protein kinase C alpha type

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Prkca	NP_035231.2
Rattus norvegicus (Norway rat)	Prkca	NP_001099183.1
Drosophila melanogaster (fruit fly)	Pkc53E	NP_725626.1
Homo sapiens (human)	PRKCA	NP_002728.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012252	XP_320290.4
Canis lupus familiaris (dog)	PRKCA	XP_005624328.1
Bos taurus (cattle)	PRKCA	NP_776860.1
Gallus gallus (chicken)	PRKCA	NP_001012822.1
Danio rerio (zebrafish)	prkca	NP_001243170.1
	PRKCA	XP_002800615.1
Gallus gallus (chicken)	LOC101749801	XP_004946286.1
Caenorhabditis elegans (roundworm)	pkc-2	NP_001024518.1
Pan troglodytes (chimpanzee)	PRKCA	XP_003315734.1
Xenopus tropicalis (tropical clawed frog)	prkca	NP_001072752.1

Gene Ontology
Bibliography

Related articles in PubMed

Protein kinase C? and integrin-linked kinase mediate the negative axon guidance effects of Sonic hedgehog.
Guo D, Standley C, Bellve K, Fogarty K, Bao ZZ
Molecular and cellular neurosciences50(1)82-92(2012 May)

Protein kinase C isotypes in signal transduction for the 1,25D3-MARRS receptor (ERp57/PDIA3) in steroid hormone-stimulated phosphate uptake.
Tunsophon S, Nemere I
Steroids75(4-5)307-13(2010 Apr)

Interleukin-6 promotes 2-deoxyglucose uptake through p44/42 MAPKs activation via Ca2+/PKC and EGF receptor in primary cultured chicken hepatocytes.
Suh HN, Lee YJ, Han HJ
Journal of cellular physiology218(3)643-52(2009 Mar)

A potential mechanism for short time exposure to hypoxia-induced DNA synthesis in primary cultured chicken hepatocytes: Correlation between Ca(2+)/PKC/MAPKs and PI3K/Akt/mTOR.
Lee SH, Lee MY, Lee JH, Han HJ
Journal of cellular biochemistry104(5)1598-611(2008 Aug)

Role of interleukin-6 in the control of DNA synthesis of hepatocytes: involvement of PKC, p44/42 MAPKs, and PPARdelta.
Suh HN, Lee SH, Lee MY, Lee YJ, Lee JH, Han HJ
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology22(5-6)673-84(2008)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PKCalpha directly phosphorylates integrin-linked kinase on threonine-173 and -181 in vitro. Dominant negative PKCalpha disrupts RGC axon pathfinding at the optic chiasm.
Title: Protein kinase C? and integrin-linked kinase mediate the negative axon guidance effects of Sonic hedgehog.

PKCalpha and PKCbeta are both involved in steroid-stimulated phosphate uptake.
Title: Protein kinase C isotypes in signal transduction for the 1,25D3-MARRS receptor (ERp57/PDIA3) in steroid hormone-stimulated phosphate uptake.

IL-6 stimulates the 2-deoxyglucose uptake through p44/42 MAPKs activation via Ca(2+)/PKC and EGF receptor in primary cultured chicken hepatocytes.
Title: Interleukin-6 promotes 2-deoxyglucose uptake through p44/42 MAPKs activation via Ca2+/PKC and EGF receptor in primary cultured chicken hepatocytes.

Short time exposure to hypoxia increases DNA synthesis in primary cultured chicken hepatocytes through cooperation of Ca2+/PKC, p38 MAPK, p44/42 MAPKs, and PI3K/Akt pathways.
Title: A potential mechanism for short time exposure to hypoxia-induced DNA synthesis in primary cultured chicken hepatocytes: Correlation between Ca(2+)/PKC/MAPKs and PI3K/Akt/mTOR.

IL-6 stimulates the proliferation of primary cultured chicken hepatocytes through PKC, p44/42 MAPKs, and PPARdelta pathways.
Title: Role of interleukin-6 in the control of DNA synthesis of hepatocytes: involvement of PKC, p44/42 MAPKs, and PPARdelta.

The following PRKCA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKCA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa25335	NM_001012804.1 Latest version!	Gallus gallus protein kinase C alpha (PRKCA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OGa16674	XM_004946229.3 Latest version!	Gallus gallus protein kinase C alpha (PRKCA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OGa145366	XM_025141605.1 Latest version!	Gallus gallus protein kinase C alpha (PRKCA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OGa16674	XM_004946229.2	Gallus gallus protein kinase C, alpha (PRKCA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRKCA ( NM_001012804.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001012822.1 Gallus gallus protein kinase C alpha (PRKCA), mRNA.

Price & Availability↑

CloneID	OGa25335
Clone ID Related Accession (Same CDS sequence)	NM_001012804.1
Accession Version	NM_001012804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-20
Organism	Gallus gallus(chicken)
Product	protein kinase C alpha type
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ851529.1. On Mar 14, 2005 this sequence version replaced XM_415682.1. ##Evidence-Data-START## Transcript exon combination :: AJ851529.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCCGACG TGTTCCCGGG CTCCGAGCCC GGCGCGGCCC CGGACGCGGC GCGGCGCTTT 
GCTCGCAAAG GGGCCCTGAG GCAGAAGAAC GTGCACGAGG TGAAGGAGCA CAAATTCATC 
GCGCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGATTT 
GGGAAACAAG GATTTCAGTG CCAAGTTTGC TGTTTTGTGG TTCATAAGAG ATGCCATGAG 
TTCGTTACCT TCTCCTGTCC TGGAGCTGAC AAGGGACCTG ACACCGATGA CCCCAGGAGC 
AAGCACAAAT TTAAGATCCA CACCTATGGG AGCCCAACCT TCTGTGACCA CTGTGGGTCC 
CTTCTTTATG GGCTCCTACA CCAAGGGATG AAATGTGACA CTTGTGATAT GAATGTGCAT 
AAGCAATGTG TGATAAACGT CCCGAGCCTC TGTGGCATGG ATCACACTGA GAAAAGGGGA 
AGGATTTATC TGAAGGCTGA AGTCACTGGT GACAAACTGG AAGTAACAGT GCGAGAAGCA 
AAAAACCTAA TTCCCATGGA TCCAAATGGG CTTTCAGATC CTTATGTTAA ACTGAAACTT 
ATCCCAGATC CCAAGAATGA AAGTAAACAA AAAACAAAAA CCTTCCGTTC TACCCTGAAT 
CCACACTGGA ATGAGTCATT CACATTTAAA TTAAAACCTA CAGACAAAGA TCGACGGCTC 
TCTGTAGAGG TCTGGGACTG GGATCGAACA ACCAGGAATG ATTTTATGGG CTCTCTTTCA 
TTTGGGGTGT CAGAGCTCAT GAAGATGCCA GCCAGTGGAT GGTACAAGCT GCTGAATCAA 
GAAGAAGGTG AATATTATAA TGTTCCAATT CCAGATGCTG ACGAGGATGG AAATGCAGAG 
CTCCGGCAGA AGTTCGAGAA AGCCAAACTT GGGCCAGCTG GTAACAAAGT CATTACTCCA 
TCAGAAGACA GGAATTCAAG TGTGCCATCC AACAATCTGG ACAGGGTGAA ACTGACAGAT 
TTCAACTTTC TTATGGTTCT TGGAAAAGGA AGCTTTGGAA AGGTGATGCT GGCGGACAGG 
AAGAATACAG AGGAGCTCTA TGCAATCAAA ATACTGAAAA AAGATGTGGT GATTCAGGAT 
GATGATGTTG AGTGTACAAT GGTTGAAAAA CGAGTGCTAG CACTGCAGGA TAAACCACCA 
TTCCTGACAC AGCTTCACTC ATGTTTCCAA ACAGTTGACC GCCTGTATTT TGTTATGGAG 
TATGTGAATG GTGGGGATCT CATGTACCAC ATTCAGCAAG TAGGAAAATT TAAGGAGCCA 
CAAGCAGTGT TCTATGCAGC TGAGATCTCA GTTGGGTTAT TCTTTCTCCA TAATAGAGGG 
ATTGTTTATA GAGATCTGAA ATTGGATAAT GTGATGTTGG ATTCAGAAGG ACACATTAAA 
ATTGCTGACT TTGGAATGTG CAAAGAACAT ATGTTAGATG GAGTAACAAC CAGGACCTTC 
TGTGGCACCC CAGATTACAT CGCACCGGAG ATCATTGCTT ATCAGCCCTA TGGGAAGTCT 
GTGGATTGGT GGGCATATGG AGTGCTGCTC TACGAGATGT TAGCTGGCCA GCCTCCATTT 
GATGGAGAAG ATGAAGATGA ACTTTTCCAG TCCATAATGG AACATAATGT TTCCTATCCA 
AAATCGCTGT CCAAAGAAGC TGTCTCCATC TGCAAGGGGC TAATGACTAA ACATCCCGCA 
AAGCGCCTTG GCTGCGGCCT CGAAGGTGAA AGAGACATCA GGGAACACGC TTTCTTCAGG 
AGAATTGACT GGGAGAAACT GGAAAACAGA GAGATCCAGC CACCTTTCAA GCCCAAAGTG 
TGTGGCAAAG GTGCTGAAAA CTTCGATAAA TTCTTCACGC GAGGACAGCC GGTGTTGACC 
CCGCCAGACC AGCTGGTCAT TGCTAACATA GATCAATCCG ATTTTGAAGG GTTCTCCTAT 
GTCAACCCCC AGTTTGTACA TCCCCTCCTA GAAAATGTAG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012822.1
CDS	68..2092
Translation	MADVFPGSEPGAAPDAARRFARKGALRQKNVHEVKEHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGSLLYGLLHQGMKCDTCDMNVHKQCVINVPSLCGMDHTEKRGRIYLKAEVTGDKLEVTVREAKNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKTFRSTLNPHWNESFTFKLKPTDKDRRLSVEVWDWDRTTRNDFMGSLSFGVSELMKMPASGWYKLLNQEEGEYYNVPIPDADEDGNAELRQKFEKAKLGPAGNKVITPSEDRNSSVPSNNLDRVKLTDFNFLMVLGKGSFGKVMLADRKNTEELYAIKILKKDVVIQDDDVECTMVEKRVLALQDKPPFLTQLHSCFQTVDRLYFVMEYVNGGDLMYHIQQVGKFKEPQAVFYAAEISVGLFFLHNRGIVYRDLKLDNVMLDSEGHIKIADFGMCKEHMLDGVTTRTFCGTPDYIAPEIIAYQPYGKSVDWWAYGVLLYEMLAGQPPFDGEDEDELFQSIMEHNVSYPKSLSKEAVSICKGLMTKHPAKRLGCGLEGERDIREHAFFRRIDWEKLENREIQPPFKPKVCGKGAENFDKFFTRGQPVLTPPDQLVIANIDQSDFEGFSYVNPQFVHPLLENVA

Target ORF information:

RefSeq Version	NM_001012804.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus protein kinase C alpha (PRKCA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012804.1

ORF Insert Sequence:

ATGGCCGACG TGTTCCCGGG CTCCGAGCCC GGCGCGGCCC CGGACGCGGC GCGGCGCTTT 
GCTCGCAAAG GGGCCCTGAG GCAGAAGAAC GTGCACGAGG TGAAGGAGCA CAAATTCATC 
GCGCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGATTT 
GGGAAACAAG GATTTCAGTG CCAAGTTTGC TGTTTTGTGG TTCATAAGAG ATGCCATGAG 
TTCGTTACCT TCTCCTGTCC TGGAGCTGAC AAGGGACCTG ACACCGATGA CCCCAGGAGC 
AAGCACAAAT TTAAGATCCA CACCTATGGG AGCCCAACCT TCTGTGACCA CTGTGGGTCC 
CTTCTTTATG GGCTCCTACA CCAAGGGATG AAATGTGACA CTTGTGATAT GAATGTGCAT 
AAGCAATGTG TGATAAACGT CCCGAGCCTC TGTGGCATGG ATCACACTGA GAAAAGGGGA 
AGGATTTATC TGAAGGCTGA AGTCACTGGT GACAAACTGG AAGTAACAGT GCGAGAAGCA 
AAAAACCTAA TTCCCATGGA TCCAAATGGG CTTTCAGATC CTTATGTTAA ACTGAAACTT 
ATCCCAGATC CCAAGAATGA AAGTAAACAA AAAACAAAAA CCTTCCGTTC TACCCTGAAT 
CCACACTGGA ATGAGTCATT CACATTTAAA TTAAAACCTA CAGACAAAGA TCGACGGCTC 
TCTGTAGAGG TCTGGGACTG GGATCGAACA ACCAGGAATG ATTTTATGGG CTCTCTTTCA 
TTTGGGGTGT CAGAGCTCAT GAAGATGCCA GCCAGTGGAT GGTACAAGCT GCTGAATCAA 
GAAGAAGGTG AATATTATAA TGTTCCAATT CCAGATGCTG ACGAGGATGG AAATGCAGAG 
CTCCGGCAGA AGTTCGAGAA AGCCAAACTT GGGCCAGCTG GTAACAAAGT CATTACTCCA 
TCAGAAGACA GGAATTCAAG TGTGCCATCC AACAATCTGG ACAGGGTGAA ACTGACAGAT 
TTCAACTTTC TTATGGTTCT TGGAAAAGGA AGCTTTGGAA AGGTGATGCT GGCGGACAGG 
AAGAATACAG AGGAGCTCTA TGCAATCAAA ATACTGAAAA AAGATGTGGT GATTCAGGAT 
GATGATGTTG AGTGTACAAT GGTTGAAAAA CGAGTGCTAG CACTGCAGGA TAAACCACCA 
TTCCTGACAC AGCTTCACTC ATGTTTCCAA ACAGTTGACC GCCTGTATTT TGTTATGGAG 
TATGTGAATG GTGGGGATCT CATGTACCAC ATTCAGCAAG TAGGAAAATT TAAGGAGCCA 
CAAGCAGTGT TCTATGCAGC TGAGATCTCA GTTGGGTTAT TCTTTCTCCA TAATAGAGGG 
ATTGTTTATA GAGATCTGAA ATTGGATAAT GTGATGTTGG ATTCAGAAGG ACACATTAAA 
ATTGCTGACT TTGGAATGTG CAAAGAACAT ATGTTAGATG GAGTAACAAC CAGGACCTTC 
TGTGGCACCC CAGATTACAT CGCACCGGAG ATCATTGCTT ATCAGCCCTA TGGGAAGTCT 
GTGGATTGGT GGGCATATGG AGTGCTGCTC TACGAGATGT TAGCTGGCCA GCCTCCATTT 
GATGGAGAAG ATGAAGATGA ACTTTTCCAG TCCATAATGG AACATAATGT TTCCTATCCA 
AAATCGCTGT CCAAAGAAGC TGTCTCCATC TGCAAGGGGC TAATGACTAA ACATCCCGCA 
AAGCGCCTTG GCTGCGGCCT CGAAGGTGAA AGAGACATCA GGGAACACGC TTTCTTCAGG 
AGAATTGACT GGGAGAAACT GGAAAACAGA GAGATCCAGC CACCTTTCAA GCCCAAAGTG 
TGTGGCAAAG GTGCTGAAAA CTTCGATAAA TTCTTCACGC GAGGACAGCC GGTGTTGACC 
CCGCCAGACC AGCTGGTCAT TGCTAACATA GATCAATCCG ATTTTGAAGG GTTCTCCTAT 
GTCAACCCCC AGTTTGTACA TCCCCTCCTA GAAAATGTAG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKCA ( XM_004946229.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004946286.1 Gallus gallus protein kinase C, alpha (PRKCA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa16674
Clone ID Related Accession (Same CDS sequence)	XM_004946229.2 , XM_004946229.3
Accession Version	XM_004946229.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	protein kinase C alpha type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764079.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004946229.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGACG TGTTCCCGGG CTCCGAGCCC GGCGCGGCCC CGGACGCGGC GCGGCGCTTT 
GCTCGCAAAG GGGCCCTGAG GCAGAAGAAC GTGCACGAGG TGAAGGAGCA CAAATTCATC 
GCGCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGATTT 
GGGAAACAAG GATTTCAGTG CCAAGTTTGC TGTTTTGTGG TTCATAAGAG ATGCCATGAG 
TTCGTTACCT TCTCCTGTCC TGGAGCTGAC AAGGGACCTG ACACCGATGA CCCCAGGAGC 
AAGCACAAAT TTAAGATCCA CACCTATGGG AGCCCAACCT TCTGTGACCA CTGTGGGTCC 
CTTCTTTATG GGCTCCTACA CCAAGGGATG AAATGTGACA CTTGTGATAT GAATGTGCAT 
AAGCAATGTG TGATAAACGT CCCGAGCCTC TGTGGCATGG ATCACACTGA GAAAAGGGGA 
AGGATTTATC TGAAGGCTGA AGTCACTGGT GACAAACTGG AAGTAACAGT GCGAGAAGCA 
AAAAACCTAA TTCCCATGGA TCCAAATGGG CTTTCAGATC CTTATGTTAA ACTGAAACTT 
ATCCCAGATC CCAAGAATGA AAGTAAACAA AAAACAAAAA CCTTCCGTTC TACCCTGAAT 
CCACACTGGA ATGAGTCATT CACATTTAAA TTAAAACCTA CAGACAAAGA TCGACGGCTC 
TCTGTAGAGG TCTGGGACTG GGATCGAACA ACCAGGAATG ATTTTATGGG CTCTCTTTCA 
TTTGGGGTGT CAGAGCTCAT GAAGATGCCA GCCAGTGGAT GGTACAAGCT GCTGAATCAA 
GAAGAAGGTG AATATTATAA TGTTCCAATT CCAGATGCTG ACGAGGATGG AAATGCAGAG 
CTCCGGCAGA AGTTCGAGGG TAAAAGAACG GGCCGAGATA AGAAAGCCAA ACTTGGGCCA 
GCTGGTAACA AAGTCATTAC TCCATCAGAA GACAGGAATT CAAGTGTGCC ATCCAACAAT 
CTGGACAGGG TGAAGCTGAC AGATTTCAAC TTTCTTATGG TTCTTGGAAA AGGAAGCTTT 
GGAAAGGTGA TGCTGGCGGA CAGGAAGAAT ACAGAGGAGC TCTATGCAAT CAAAATACTG 
AAAAAAGATG TGGTGATTCA GGATGATGAT GTTGAGTGTA CAATGGTTGA AAAACGAGTG 
CTAGCACTGC AGGATAAACC ACCATTCCTG ACACAGCTTC ACTCATGTTT CCAAACAGTT 
GACCGCCTGT ATTTTGTTAT GGAGTATGTG AATGGTGGGG ATCTCATGTA CCACATTCAG 
CAAGTAGGAA AATTTAAGGA GCCACAAGCA GTGTTCTATG CAGCTGAGAT CTCAGTTGGG 
TTATTCTTTC TCCATAATAG AGGGATTGTT TATAGAGATC TGAAATTGGA TAATGTGATG 
TTGGATTCAG AAGGACACAT TAAAATTGCT GACTTTGGAA TGTGCAAAGA ACATATGTTA 
GATGGAGTAA CAACCAGGAC CTTCTGTGGC ACCCCAGATT ACATCGCACC GGAGATCATT 
GCTTATCAGC CCTATGGGAA GTCTGTGGAT TGGTGGGCAT ATGGAGTGCT GCTCTACGAG 
ATGTTAGCTG GCCAGCCTCC ATTTGATGGA GAAGACGAAG ATGAACTTTT CCAGTCCATA 
ATGGAACATA ATGTTTCCTA TCCAAAATCG CTGTCCAAAG AAGCTGTCTC CATCTGCAAG 
GGGCTAATGA CTAAACATCC CGCAAAGCGC CTTGGCTGCG GCCTCGAAGG TGAAAGAGAC 
ATCAGGGAAC ACGCTTTCTT CAGGAGAATT GACTGGGAGA AACTGGAAAA CAGAGAGATC 
CAGCCACCTT TCAAGCCCAA AGTGTGTGGC AAAGGTGCTG AAAACTTCGA TAAATTCTTC 
ACGCGAGGAC AGCCGGTGTT GACCCCGCCA GACCAGCTGG TTATTGCTAA CATAGATCAA 
TCCGATTTTG AAGGGTTCTC CTATGTCAAC CCCCAGTTTG TACATCCCCT CCTAGAAAAT 
GTAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004946286.1
CDS	176..2224
Translation	MADVFPGSEPGAAPDAARRFARKGALRQKNVHEVKEHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGSLLYGLLHQGMKCDTCDMNVHKQCVINVPSLCGMDHTEKRGRIYLKAEVTGDKLEVTVREAKNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKTFRSTLNPHWNESFTFKLKPTDKDRRLSVEVWDWDRTTRNDFMGSLSFGVSELMKMPASGWYKLLNQEEGEYYNVPIPDADEDGNAELRQKFEGKRTGRDKKAKLGPAGNKVITPSEDRNSSVPSNNLDRVKLTDFNFLMVLGKGSFGKVMLADRKNTEELYAIKILKKDVVIQDDDVECTMVEKRVLALQDKPPFLTQLHSCFQTVDRLYFVMEYVNGGDLMYHIQQVGKFKEPQAVFYAAEISVGLFFLHNRGIVYRDLKLDNVMLDSEGHIKIADFGMCKEHMLDGVTTRTFCGTPDYIAPEIIAYQPYGKSVDWWAYGVLLYEMLAGQPPFDGEDEDELFQSIMEHNVSYPKSLSKEAVSICKGLMTKHPAKRLGCGLEGERDIREHAFFRRIDWEKLENREIQPPFKPKVCGKGAENFDKFFTRGQPVLTPPDQLVIANIDQSDFEGFSYVNPQFVHPLLENVA

Target ORF information:

RefSeq Version	XM_004946229.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus protein kinase C, alpha (PRKCA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004946229.2

ORF Insert Sequence:

ATGGCCGACG TGTTCCCGGG CTCCGAGCCC GGCGCGGCCC CGGACGCGGC GCGGCGCTTT 
GCTCGCAAAG GGGCCCTGAG GCAGAAGAAC GTGCACGAGG TGAAGGAGCA CAAATTCATC 
GCGCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGATTT 
GGGAAACAAG GATTTCAGTG CCAAGTTTGC TGTTTTGTGG TTCATAAGAG ATGCCATGAG 
TTCGTTACCT TCTCCTGTCC TGGAGCTGAC AAGGGACCTG ACACCGATGA CCCCAGGAGC 
AAGCACAAAT TTAAGATCCA CACCTATGGG AGCCCAACCT TCTGTGACCA CTGTGGGTCC 
CTTCTTTATG GGCTCCTACA CCAAGGGATG AAATGTGACA CTTGTGATAT GAATGTGCAT 
AAGCAATGTG TGATAAACGT CCCGAGCCTC TGTGGCATGG ATCACACTGA GAAAAGGGGA 
AGGATTTATC TGAAGGCTGA AGTCACTGGT GACAAACTGG AAGTAACAGT GCGAGAAGCA 
AAAAACCTAA TTCCCATGGA TCCAAATGGG CTTTCAGATC CTTATGTTAA ACTGAAACTT 
ATCCCAGATC CCAAGAATGA AAGTAAACAA AAAACAAAAA CCTTCCGTTC TACCCTGAAT 
CCACACTGGA ATGAGTCATT CACATTTAAA TTAAAACCTA CAGACAAAGA TCGACGGCTC 
TCTGTAGAGG TCTGGGACTG GGATCGAACA ACCAGGAATG ATTTTATGGG CTCTCTTTCA 
TTTGGGGTGT CAGAGCTCAT GAAGATGCCA GCCAGTGGAT GGTACAAGCT GCTGAATCAA 
GAAGAAGGTG AATATTATAA TGTTCCAATT CCAGATGCTG ACGAGGATGG AAATGCAGAG 
CTCCGGCAGA AGTTCGAGGG TAAAAGAACG GGCCGAGATA AGAAAGCCAA ACTTGGGCCA 
GCTGGTAACA AAGTCATTAC TCCATCAGAA GACAGGAATT CAAGTGTGCC ATCCAACAAT 
CTGGACAGGG TGAAGCTGAC AGATTTCAAC TTTCTTATGG TTCTTGGAAA AGGAAGCTTT 
GGAAAGGTGA TGCTGGCGGA CAGGAAGAAT ACAGAGGAGC TCTATGCAAT CAAAATACTG 
AAAAAAGATG TGGTGATTCA GGATGATGAT GTTGAGTGTA CAATGGTTGA AAAACGAGTG 
CTAGCACTGC AGGATAAACC ACCATTCCTG ACACAGCTTC ACTCATGTTT CCAAACAGTT 
GACCGCCTGT ATTTTGTTAT GGAGTATGTG AATGGTGGGG ATCTCATGTA CCACATTCAG 
CAAGTAGGAA AATTTAAGGA GCCACAAGCA GTGTTCTATG CAGCTGAGAT CTCAGTTGGG 
TTATTCTTTC TCCATAATAG AGGGATTGTT TATAGAGATC TGAAATTGGA TAATGTGATG 
TTGGATTCAG AAGGACACAT TAAAATTGCT GACTTTGGAA TGTGCAAAGA ACATATGTTA 
GATGGAGTAA CAACCAGGAC CTTCTGTGGC ACCCCAGATT ACATCGCACC GGAGATCATT 
GCTTATCAGC CCTATGGGAA GTCTGTGGAT TGGTGGGCAT ATGGAGTGCT GCTCTACGAG 
ATGTTAGCTG GCCAGCCTCC ATTTGATGGA GAAGACGAAG ATGAACTTTT CCAGTCCATA 
ATGGAACATA ATGTTTCCTA TCCAAAATCG CTGTCCAAAG AAGCTGTCTC CATCTGCAAG 
GGGCTAATGA CTAAACATCC CGCAAAGCGC CTTGGCTGCG GCCTCGAAGG TGAAAGAGAC 
ATCAGGGAAC ACGCTTTCTT CAGGAGAATT GACTGGGAGA AACTGGAAAA CAGAGAGATC 
CAGCCACCTT TCAAGCCCAA AGTGTGTGGC AAAGGTGCTG AAAACTTCGA TAAATTCTTC 
ACGCGAGGAC AGCCGGTGTT GACCCCGCCA GACCAGCTGG TTATTGCTAA CATAGATCAA 
TCCGATTTTG AAGGGTTCTC CTATGTCAAC CCCCAGTTTG TACATCCCCT CCTAGAAAAT 
GTAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKCA ( XM_004946229.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004946286.1 Gallus gallus protein kinase C alpha (PRKCA), transcript variant X1, mRNA.

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CloneID	OGa16674
Clone ID Related Accession (Same CDS sequence)	XM_004946229.2 , XM_004946229.3
Accession Version	XM_004946229.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	protein kinase C alpha type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006105.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004946229.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGACG TGTTCCCGGG CTCCGAGCCC GGCGCGGCCC CGGACGCGGC GCGGCGCTTT 
GCTCGCAAAG GGGCCCTGAG GCAGAAGAAC GTGCACGAGG TGAAGGAGCA CAAATTCATC 
GCGCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGATTT 
GGGAAACAAG GATTTCAGTG CCAAGTTTGC TGTTTTGTGG TTCATAAGAG ATGCCATGAG 
TTCGTTACCT TCTCCTGTCC TGGAGCTGAC AAGGGACCTG ACACCGATGA CCCCAGGAGC 
AAGCACAAAT TTAAGATCCA CACCTATGGG AGCCCAACCT TCTGTGACCA CTGTGGGTCC 
CTTCTTTATG GGCTCCTACA CCAAGGGATG AAATGTGACA CTTGTGATAT GAATGTGCAT 
AAGCAATGTG TGATAAACGT CCCGAGCCTC TGTGGCATGG ATCACACTGA GAAAAGGGGA 
AGGATTTATC TGAAGGCTGA AGTCACTGGT GACAAACTGG AAGTAACAGT GCGAGAAGCA 
AAAAACCTAA TTCCCATGGA TCCAAATGGG CTTTCAGATC CTTATGTTAA ACTGAAACTT 
ATCCCAGATC CCAAGAATGA AAGTAAACAA AAAACAAAAA CCTTCCGTTC TACCCTGAAT 
CCACACTGGA ATGAGTCATT CACATTTAAA TTAAAACCTA CAGACAAAGA TCGACGGCTC 
TCTGTAGAGG TCTGGGACTG GGATCGAACA ACCAGGAATG ATTTTATGGG CTCTCTTTCA 
TTTGGGGTGT CAGAGCTCAT GAAGATGCCA GCCAGTGGAT GGTACAAGCT GCTGAATCAA 
GAAGAAGGTG AATATTATAA TGTTCCAATT CCAGATGCTG ACGAGGATGG AAATGCAGAG 
CTCCGGCAGA AGTTCGAGGG TAAAAGAACG GGCCGAGATA AGAAAGCCAA ACTTGGGCCA 
GCTGGTAACA AAGTCATTAC TCCATCAGAA GACAGGAATT CAAGTGTGCC ATCCAACAAT 
CTGGACAGGG TGAAGCTGAC AGATTTCAAC TTTCTTATGG TTCTTGGAAA AGGAAGCTTT 
GGAAAGGTGA TGCTGGCGGA CAGGAAGAAT ACAGAGGAGC TCTATGCAAT CAAAATACTG 
AAAAAAGATG TGGTGATTCA GGATGATGAT GTTGAGTGTA CAATGGTTGA AAAACGAGTG 
CTAGCACTGC AGGATAAACC ACCATTCCTG ACACAGCTTC ACTCATGTTT CCAAACAGTT 
GACCGCCTGT ATTTTGTTAT GGAGTATGTG AATGGTGGGG ATCTCATGTA CCACATTCAG 
CAAGTAGGAA AATTTAAGGA GCCACAAGCA GTGTTCTATG CAGCTGAGAT CTCAGTTGGG 
TTATTCTTTC TCCATAATAG AGGGATTGTT TATAGAGATC TGAAATTGGA TAATGTGATG 
TTGGATTCAG AAGGACACAT TAAAATTGCT GACTTTGGAA TGTGCAAAGA ACATATGTTA 
GATGGAGTAA CAACCAGGAC CTTCTGTGGC ACCCCAGATT ACATCGCACC GGAGATCATT 
GCTTATCAGC CCTATGGGAA GTCTGTGGAT TGGTGGGCAT ATGGAGTGCT GCTCTACGAG 
ATGTTAGCTG GCCAGCCTCC ATTTGATGGA GAAGACGAAG ATGAACTTTT CCAGTCCATA 
ATGGAACATA ATGTTTCCTA TCCAAAATCG CTGTCCAAAG AAGCTGTCTC CATCTGCAAG 
GGGCTAATGA CTAAACATCC CGCAAAGCGC CTTGGCTGCG GCCTCGAAGG TGAAAGAGAC 
ATCAGGGAAC ACGCTTTCTT CAGGAGAATT GACTGGGAGA AACTGGAAAA CAGAGAGATC 
CAGCCACCTT TCAAGCCCAA AGTGTGTGGC AAAGGTGCTG AAAACTTCGA TAAATTCTTC 
ACGCGAGGAC AGCCGGTGTT GACCCCGCCA GACCAGCTGG TTATTGCTAA CATAGATCAA 
TCCGATTTTG AAGGGTTCTC CTATGTCAAC CCCCAGTTTG TACATCCCCT CCTAGAAAAT 
GTAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004946286.1
CDS	202..2250
Translation	MADVFPGSEPGAAPDAARRFARKGALRQKNVHEVKEHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGSLLYGLLHQGMKCDTCDMNVHKQCVINVPSLCGMDHTEKRGRIYLKAEVTGDKLEVTVREAKNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKTFRSTLNPHWNESFTFKLKPTDKDRRLSVEVWDWDRTTRNDFMGSLSFGVSELMKMPASGWYKLLNQEEGEYYNVPIPDADEDGNAELRQKFEGKRTGRDKKAKLGPAGNKVITPSEDRNSSVPSNNLDRVKLTDFNFLMVLGKGSFGKVMLADRKNTEELYAIKILKKDVVIQDDDVECTMVEKRVLALQDKPPFLTQLHSCFQTVDRLYFVMEYVNGGDLMYHIQQVGKFKEPQAVFYAAEISVGLFFLHNRGIVYRDLKLDNVMLDSEGHIKIADFGMCKEHMLDGVTTRTFCGTPDYIAPEIIAYQPYGKSVDWWAYGVLLYEMLAGQPPFDGEDEDELFQSIMEHNVSYPKSLSKEAVSICKGLMTKHPAKRLGCGLEGERDIREHAFFRRIDWEKLENREIQPPFKPKVCGKGAENFDKFFTRGQPVLTPPDQLVIANIDQSDFEGFSYVNPQFVHPLLENVA

Target ORF information:

RefSeq Version	XM_004946229.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus protein kinase C alpha (PRKCA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004946229.3

ORF Insert Sequence:

ATGGCCGACG TGTTCCCGGG CTCCGAGCCC GGCGCGGCCC CGGACGCGGC GCGGCGCTTT 
GCTCGCAAAG GGGCCCTGAG GCAGAAGAAC GTGCACGAGG TGAAGGAGCA CAAATTCATC 
GCGCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGATTT 
GGGAAACAAG GATTTCAGTG CCAAGTTTGC TGTTTTGTGG TTCATAAGAG ATGCCATGAG 
TTCGTTACCT TCTCCTGTCC TGGAGCTGAC AAGGGACCTG ACACCGATGA CCCCAGGAGC 
AAGCACAAAT TTAAGATCCA CACCTATGGG AGCCCAACCT TCTGTGACCA CTGTGGGTCC 
CTTCTTTATG GGCTCCTACA CCAAGGGATG AAATGTGACA CTTGTGATAT GAATGTGCAT 
AAGCAATGTG TGATAAACGT CCCGAGCCTC TGTGGCATGG ATCACACTGA GAAAAGGGGA 
AGGATTTATC TGAAGGCTGA AGTCACTGGT GACAAACTGG AAGTAACAGT GCGAGAAGCA 
AAAAACCTAA TTCCCATGGA TCCAAATGGG CTTTCAGATC CTTATGTTAA ACTGAAACTT 
ATCCCAGATC CCAAGAATGA AAGTAAACAA AAAACAAAAA CCTTCCGTTC TACCCTGAAT 
CCACACTGGA ATGAGTCATT CACATTTAAA TTAAAACCTA CAGACAAAGA TCGACGGCTC 
TCTGTAGAGG TCTGGGACTG GGATCGAACA ACCAGGAATG ATTTTATGGG CTCTCTTTCA 
TTTGGGGTGT CAGAGCTCAT GAAGATGCCA GCCAGTGGAT GGTACAAGCT GCTGAATCAA 
GAAGAAGGTG AATATTATAA TGTTCCAATT CCAGATGCTG ACGAGGATGG AAATGCAGAG 
CTCCGGCAGA AGTTCGAGGG TAAAAGAACG GGCCGAGATA AGAAAGCCAA ACTTGGGCCA 
GCTGGTAACA AAGTCATTAC TCCATCAGAA GACAGGAATT CAAGTGTGCC ATCCAACAAT 
CTGGACAGGG TGAAGCTGAC AGATTTCAAC TTTCTTATGG TTCTTGGAAA AGGAAGCTTT 
GGAAAGGTGA TGCTGGCGGA CAGGAAGAAT ACAGAGGAGC TCTATGCAAT CAAAATACTG 
AAAAAAGATG TGGTGATTCA GGATGATGAT GTTGAGTGTA CAATGGTTGA AAAACGAGTG 
CTAGCACTGC AGGATAAACC ACCATTCCTG ACACAGCTTC ACTCATGTTT CCAAACAGTT 
GACCGCCTGT ATTTTGTTAT GGAGTATGTG AATGGTGGGG ATCTCATGTA CCACATTCAG 
CAAGTAGGAA AATTTAAGGA GCCACAAGCA GTGTTCTATG CAGCTGAGAT CTCAGTTGGG 
TTATTCTTTC TCCATAATAG AGGGATTGTT TATAGAGATC TGAAATTGGA TAATGTGATG 
TTGGATTCAG AAGGACACAT TAAAATTGCT GACTTTGGAA TGTGCAAAGA ACATATGTTA 
GATGGAGTAA CAACCAGGAC CTTCTGTGGC ACCCCAGATT ACATCGCACC GGAGATCATT 
GCTTATCAGC CCTATGGGAA GTCTGTGGAT TGGTGGGCAT ATGGAGTGCT GCTCTACGAG 
ATGTTAGCTG GCCAGCCTCC ATTTGATGGA GAAGACGAAG ATGAACTTTT CCAGTCCATA 
ATGGAACATA ATGTTTCCTA TCCAAAATCG CTGTCCAAAG AAGCTGTCTC CATCTGCAAG 
GGGCTAATGA CTAAACATCC CGCAAAGCGC CTTGGCTGCG GCCTCGAAGG TGAAAGAGAC 
ATCAGGGAAC ACGCTTTCTT CAGGAGAATT GACTGGGAGA AACTGGAAAA CAGAGAGATC 
CAGCCACCTT TCAAGCCCAA AGTGTGTGGC AAAGGTGCTG AAAACTTCGA TAAATTCTTC 
ACGCGAGGAC AGCCGGTGTT GACCCCGCCA GACCAGCTGG TTATTGCTAA CATAGATCAA 
TCCGATTTTG AAGGGTTCTC CTATGTCAAC CCCCAGTTTG TACATCCCCT CCTAGAAAAT 
GTAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKCA ( XM_025141605.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024997373.1 Gallus gallus protein kinase C alpha (PRKCA), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa145366
Clone ID Related Accession (Same CDS sequence)	XM_025141605.1
Accession Version	XM_025141605.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	protein kinase C alpha type isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006105.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGTG ACACTTGTGA TATGAATGTG CATAAGCAAT GTGTGATAAA CGTCCCGAGC 
CTCTGTGGCA TGGATCACAC TGAGAAAAGG GGAAGGATTT ATCTGAAGGC TGAAGTCACT 
GGTGACAAAC TGGAAGTAAC AGTGCGAGAA GCAAAAAACC TAATTCCCAT GGATCCAAAT 
GGGCTTTCAG ATCCTTATGT TAAACTGAAA CTTATCCCAG ATCCCAAGAA TGAAAGTAAA 
CAAAAAACAA AAACCTTCCG TTCTACCCTG AATCCACACT GGAATGAGTC ATTCACATTT 
AAATTAAAAC CTACAGACAA AGATCGACGG CTCTCTGTAG AGGTCTGGGA CTGGGATCGA 
ACAACCAGGA ATGATTTTAT GGGCTCTCTT TCATTTGGGG TGTCAGAGCT CATGAAGATG 
CCAGCCAGTG GATGGTACAA GCTGCTGAAT CAAGAAGAAG GTGAATATTA TAATGTTCCA 
ATTCCAGATG CTGACGAGGA TGGAAATGCA GAGCTCCGGC AGAAGTTCGA GGGTAAAAGA 
ACGGGCCGAG ATAAGAAAGC CAAACTTGGG CCAGCTGGTA ACAAAGTCAT TACTCCATCA 
GAAGACAGGA ATTCAAGTGT GCCATCCAAC AATCTGGACA GGGTGAAGCT GACAGATTTC 
AACTTTCTTA TGGTTCTTGG AAAAGGAAGC TTTGGAAAGG TGATGCTGGC GGACAGGAAG 
AATACAGAGG AGCTCTATGC AATCAAAATA CTGAAAAAAG ATGTGGTGAT TCAGGATGAT 
GATGTTGAGT GTACAATGGT TGAAAAACGA GTGCTAGCAC TGCAGGATAA ACCACCATTC 
CTGACACAGC TTCACTCATG TTTCCAAACA GTTGACCGCC TGTATTTTGT TATGGAGTAT 
GTGAATGGTG GGGATCTCAT GTACCACATT CAGCAAGTAG GAAAATTTAA GGAGCCACAA 
GCAGTGTTCT ATGCAGCTGA GATCTCAGTT GGGTTATTCT TTCTCCATAA TAGAGGGATT 
GTTTATAGAG ATCTGAAATT GGATAATGTG ATGTTGGATT CAGAAGGACA CATTAAAATT 
GCTGACTTTG GAATGTGCAA AGAACATATG TTAGATGGAG TAACAACCAG GACCTTCTGT 
GGCACCCCAG ATTACATCGC ACCGGAGATC ATTGCTTATC AGCCCTATGG GAAGTCTGTG 
GATTGGTGGG CATATGGAGT GCTGCTCTAC GAGATGTTAG CTGGCCAGCC TCCATTTGAT 
GGAGAAGACG AAGATGAACT TTTCCAGTCC ATAATGGAAC ATAATGTTTC CTATCCAAAA 
TCGCTGTCCA AAGAAGCTGT CTCCATCTGC AAGGGGCTAA TGACTAAACA TCCCGCAAAG 
CGCCTTGGCT GCGGCCTCGA AGGTGAAAGA GACATCAGGG AACACGCTTT CTTCAGGAGA 
ATTGACTGGG AGAAACTGGA AAACAGAGAG ATCCAGCCAC CTTTCAAGCC CAAAGTGTGT 
GGCAAAGGTG CTGAAAACTT CGATAAATTC TTCACGCGAG GACAGCCGGT GTTGACCCCG 
CCAGACCAGC TGGTTATTGC TAACATAGAT CAATCCGATT TTGAAGGGTT CTCCTATGTC 
AACCCCCAGT TTGTACATCC CCTCCTAGAA AATGTAGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024997373.1
CDS	422..2083
Translation	MKCDTCDMNVHKQCVINVPSLCGMDHTEKRGRIYLKAEVTGDKLEVTVREAKNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKTFRSTLNPHWNESFTFKLKPTDKDRRLSVEVWDWDRTTRNDFMGSLSFGVSELMKMPASGWYKLLNQEEGEYYNVPIPDADEDGNAELRQKFEGKRTGRDKKAKLGPAGNKVITPSEDRNSSVPSNNLDRVKLTDFNFLMVLGKGSFGKVMLADRKNTEELYAIKILKKDVVIQDDDVECTMVEKRVLALQDKPPFLTQLHSCFQTVDRLYFVMEYVNGGDLMYHIQQVGKFKEPQAVFYAAEISVGLFFLHNRGIVYRDLKLDNVMLDSEGHIKIADFGMCKEHMLDGVTTRTFCGTPDYIAPEIIAYQPYGKSVDWWAYGVLLYEMLAGQPPFDGEDEDELFQSIMEHNVSYPKSLSKEAVSICKGLMTKHPAKRLGCGLEGERDIREHAFFRRIDWEKLENREIQPPFKPKVCGKGAENFDKFFTRGQPVLTPPDQLVIANIDQSDFEGFSYVNPQFVHPLLENVA

Target ORF information:

RefSeq Version	XM_025141605.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus protein kinase C alpha (PRKCA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025141605.1

ORF Insert Sequence:

ATGAAATGTG ACACTTGTGA TATGAATGTG CATAAGCAAT GTGTGATAAA CGTCCCGAGC 
CTCTGTGGCA TGGATCACAC TGAGAAAAGG GGAAGGATTT ATCTGAAGGC TGAAGTCACT 
GGTGACAAAC TGGAAGTAAC AGTGCGAGAA GCAAAAAACC TAATTCCCAT GGATCCAAAT 
GGGCTTTCAG ATCCTTATGT TAAACTGAAA CTTATCCCAG ATCCCAAGAA TGAAAGTAAA 
CAAAAAACAA AAACCTTCCG TTCTACCCTG AATCCACACT GGAATGAGTC ATTCACATTT 
AAATTAAAAC CTACAGACAA AGATCGACGG CTCTCTGTAG AGGTCTGGGA CTGGGATCGA 
ACAACCAGGA ATGATTTTAT GGGCTCTCTT TCATTTGGGG TGTCAGAGCT CATGAAGATG 
CCAGCCAGTG GATGGTACAA GCTGCTGAAT CAAGAAGAAG GTGAATATTA TAATGTTCCA 
ATTCCAGATG CTGACGAGGA TGGAAATGCA GAGCTCCGGC AGAAGTTCGA GGGTAAAAGA 
ACGGGCCGAG ATAAGAAAGC CAAACTTGGG CCAGCTGGTA ACAAAGTCAT TACTCCATCA 
GAAGACAGGA ATTCAAGTGT GCCATCCAAC AATCTGGACA GGGTGAAGCT GACAGATTTC 
AACTTTCTTA TGGTTCTTGG AAAAGGAAGC TTTGGAAAGG TGATGCTGGC GGACAGGAAG 
AATACAGAGG AGCTCTATGC AATCAAAATA CTGAAAAAAG ATGTGGTGAT TCAGGATGAT 
GATGTTGAGT GTACAATGGT TGAAAAACGA GTGCTAGCAC TGCAGGATAA ACCACCATTC 
CTGACACAGC TTCACTCATG TTTCCAAACA GTTGACCGCC TGTATTTTGT TATGGAGTAT 
GTGAATGGTG GGGATCTCAT GTACCACATT CAGCAAGTAG GAAAATTTAA GGAGCCACAA 
GCAGTGTTCT ATGCAGCTGA GATCTCAGTT GGGTTATTCT TTCTCCATAA TAGAGGGATT 
GTTTATAGAG ATCTGAAATT GGATAATGTG ATGTTGGATT CAGAAGGACA CATTAAAATT 
GCTGACTTTG GAATGTGCAA AGAACATATG TTAGATGGAG TAACAACCAG GACCTTCTGT 
GGCACCCCAG ATTACATCGC ACCGGAGATC ATTGCTTATC AGCCCTATGG GAAGTCTGTG 
GATTGGTGGG CATATGGAGT GCTGCTCTAC GAGATGTTAG CTGGCCAGCC TCCATTTGAT 
GGAGAAGACG AAGATGAACT TTTCCAGTCC ATAATGGAAC ATAATGTTTC CTATCCAAAA 
TCGCTGTCCA AAGAAGCTGT CTCCATCTGC AAGGGGCTAA TGACTAAACA TCCCGCAAAG 
CGCCTTGGCT GCGGCCTCGA AGGTGAAAGA GACATCAGGG AACACGCTTT CTTCAGGAGA 
ATTGACTGGG AGAAACTGGA AAACAGAGAG ATCCAGCCAC CTTTCAAGCC CAAAGTGTGT 
GGCAAAGGTG CTGAAAACTT CGATAAATTC TTCACGCGAG GACAGCCGGT GTTGACCCCG 
CCAGACCAGC TGGTTATTGC TAACATAGAT CAATCCGATT TTGAAGGGTT CTCCTATGTC 
AACCCCCAGT TTGTACATCC CCTCCTAGAA AATGTAGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Protein kinase C? and integrin-linked kinase mediate the negative axon guidance effects of Sonic hedgehog.
Molecular and cellular neurosciences50(1)82-92(2012 May)
Guo D,Standley C,Bellve K,Fogarty K,Bao ZZ

Protein kinase C isotypes in signal transduction for the 1,25D3-MARRS receptor (ERp57/PDIA3) in steroid hormone-stimulated phosphate uptake.
Steroids75(4-5)307-13(2010 Apr)
Tunsophon S,Nemere I

Interleukin-6 promotes 2-deoxyglucose uptake through p44/42 MAPKs activation via Ca2+/PKC and EGF receptor in primary cultured chicken hepatocytes.
Journal of cellular physiology218(3)643-52(2009 Mar)
Suh HN,Lee YJ,Han HJ

A potential mechanism for short time exposure to hypoxia-induced DNA synthesis in primary cultured chicken hepatocytes: Correlation between Ca(2+)/PKC/MAPKs and PI3K/Akt/mTOR.
Journal of cellular biochemistry104(5)1598-611(2008 Aug)
Lee SH,Lee MY,Lee JH,Han HJ

Role of interleukin-6 in the control of DNA synthesis of hepatocytes: involvement of PKC, p44/42 MAPKs, and PPARdelta.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology22(5-6)673-84(2008)
Suh HN,Lee SH,Lee MY,Lee YJ,Lee JH,Han HJ

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PRKCA cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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