TXNRD3 cDNA ORF clone, Gallus gallus(Chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	TXNRD3
Entrez Gene ID	416031
Full Name	thioredoxin reductase 3
General protein information	Preferred Names thioredoxin reductase 3 Names thioredoxin reductase 3
Gene Type	protein-coding
Organism	Gallus gallus(Chicken)
Summary	The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals and some vertebrates. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes the third TrxR, which unlike the other two isozymes, contains an additional N-terminal glutaredoxin (Grx) domain, and shows highest expression in testis. The Grx domain allows this isozyme to participate in both Trx and glutathione systems. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001122777.2	NP_001116249.1	thioredoxin reductase 3
NM_001122777.1	NP_001116249.1	thioredoxin reductase 3

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	txnrd1	NP_898895.2
Bos taurus (cattle)	TXNRD3	XP_005223242.1
Rattus norvegicus (Norway rat)	Txnrd3	NP_001171641.1
Caenorhabditis elegans (roundworm)	trxr-1	NP_501085.3
Homo sapiens (human)	TXNRD3	NP_443115.1
Pan troglodytes (chimpanzee)	TXNRD3	NP_001243146.1
Canis lupus familiaris (dog)	TXNRD3	NP_001116250.1
Mus musculus (house mouse)	Txnrd3	NP_001171529.1
Gallus gallus (chicken)	TXNRD3	NP_001116249.1

Gene Ontology
Bibliography

Related articles in PubMed

Selenoprotein Gene Nomenclature.
Gladyshev VN, Arn?r ES, Berry MJ, Brigelius-Floh? R, Bruford EA, Burk RF, Carlson BA, Castellano S, Chavatte L, Conrad M, Copeland PR, Diamond AM, Driscoll DM, Ferreiro A, Floh? L, Green FR, Guig? R, Handy DE, Hatfield DL, Hesketh J, Hoffmann PR, Holmgren A, Hondal RJ, Howard MT, Huang K, Kim HY, Kim IY, K?hrle J, Krol A, Kryukov GV, Lee BJ, Lee BC, Lei XG, Liu Q, Lescure A, Lobanov AV, Loscalzo J, Maiorino M, Mariotti M, Sandeep Prabhu K, Rayman MP, Rozovsky S, Salinas G, Schmidt EE, Schomburg L, Schweizer U, Simonovi? M, Sunde RA, Tsuji PA, Tweedie S, Ursini F, Whanger PD, Zhang Y
The Journal of biological chemistry291(46)24036-24040(2016 Nov)

Dietary Selenium Status Regulates the Transcriptions of Selenoproteome and Activities of Selenoenzymes in Chicken Kidney at Low or Super-nutritional Levels.
Xu JX, Zhang C, Cao CY, Zhu SY, Li H, Sun YC, Li JL
Biological trace element research170(2)438-48(2016 Apr)

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

Non-coding RNAs revealed during identification of genes involved in chicken immune responses.
Ahanda ML, Ruby T, Wittzell H, Bed'Hom B, Chauss? AM, Morin V, Oudin A, Chevalier C, Young JR, Zoorob R
Immunogenetics61(1)55-70(2009 Jan)

Gene expression profiling of chicken primordial germ cell ESTs.
Han JY, Park TS, Kim JN, Kim MA, Lim D, Lim JM, Kim H
BMC genomics7220(2006 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dietary low Se downregulated the mRNA expressions of Gpx1-4, Txnrd3, Sepn1, Selw, Sepx1, Selh, and SEPSECS.
Title: Dietary Selenium Status Regulates the Transcriptions of Selenoproteome and Activities of Selenoenzymes in Chicken Kidney at Low or Super-nutritional Levels.

The following TXNRD3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TXNRD3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa26638	NM_001122777.2 Latest version!	Gallus gallus thioredoxin reductase 3 (TXNRD3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
OGa26638	NM_001122777.1	Gallus gallus thioredoxin reductase 3 (TXNRD3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TXNRD3 ( NM_001122777.2 ) cDNA ORF clone, Gallus gallus(Chicken) -> NP_001116249.1 Gallus gallus thioredoxin reductase 3 (TXNRD3), mRNA.

Price & Availability↑

CloneID	OGa26638
Clone ID Related Accession (Same CDS sequence)	NM_001122777.2 , NM_001122777.1
Accession Version	NM_001122777.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-11
Organism	Gallus gallus(chicken)
Product	thioredoxin reductase 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AM071104.1, AADN05000356.1, BU210630.1, AL584253.1, BI067020.1, CF258103.1, CD740317.1 and DR411839.1. On Jul 7, 2017 this sequence version replaced NM_001122777.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: ERR2368565.134082.1, ERR2368565.192739.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2201358, SAMEA2201361 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCGCCGC CCGGGCAGAC ACAGCTCCCA GACTGGGACG GCCTGAAGCT CCGCGTGCGG 
ACCCTCATCG CCACGCACCG CGTCATGATC TTCAGCAAGA GCTACTGCCC CTACTGCCAT 
CGGGTGAAAG AACTCTTCAG CTCTCTAGGT GTACAGTATT ATGCTTTGGA ACTGGATGTA 
ACTGATGATG GACCCAGTAT TCAGCAAGTG TTAGCAGAGC TAACTAATCA GAGGACGGTT 
CCTAATGTTT TTATCAATGG AAAACATATA GGTGGCTGTG ATGCAACTTA CAAGGCTTAT 
GAGAATGGAA CACTGCAAAG AATTCTTGGG GATGTCAAAG ATGCAGAAAC CTATGATTAT 
GACCTCATTG TTATTGGTGG TGGCTCAGGC GGACTCGCTT GTTCCAAGGA AGCTGCTACC 
TTGGGAAAGA AAGTGATGGT ATTAGATTAT GTTGTGCCAA CACCCCTGGG AACCTCCTGG 
GGACTTGGTG GTACATGTGT AAATGTAGGC TGTATTCCTA AGAAGCTAAT GCATCAAGCG 
GCTCTTCTGG GGCAGGCACT CAAAGATTCA AGGGCATACG GCTGGCAGTA TGATGAACAA 
GTTAAACACA ACTGGGAGAT CATGGTAGAA GCAGTTCAAA ACTACATTGG CTCTTTAAAC 
TGGGGCTATC GATTGTCCTT AAGAGAGAAA TCTGTGACAT ACCAAAATTC CTATGGAGAA 
TTTGTTGAAC CACACAAAAT CAAGGCAACT AATAGAAAAG GACAAGTAAC CTATCACACA 
GCAGAGACTT TTGTCCTGGC AACAGGAGAA AGGCCTAGAT ATCTGGGTAT TCCTGGAGAT 
AAAGAATACT GTATTACAAG TGATGACCTC TTCTCCCTGC CTTACTGCCC TGGCAAAACG 
CTAGTTGTGG GTGCTTCCTA TGTGGCACTG GAGTGCGCAG GATTTCTTGC TGGTTTAGGT 
CTAGATGTCA CAGTAATGGT GCGTTCCATA CTCCTTCGAG GCTTTGACCA AGAGATGGCA 
GAAAAAATAG GTGCCCACAT GGAAACACAT GGTGTAACGT TCATCAGAAA GTTTGTACCT 
ACTCAGGTGG AAAGGCTGGA GGATGGCACA CCGGGAAGAC TAAAAGTGAC AGCAAAGTCT 
ACTGAGGGTC CAGAATTTTT TGAAGGAGAA TATAACACTG TTTTGATAGC CATTGGTCGG 
GATGCATGTA CCAGAAATAT TGGCTTACAG ACAATTGGTG TCAAAATCAA TGAGAAGAAT 
GGGAAAGTAC CTGTAAATGA TGAAGAACGA ACCAATGTGC CTTATGTTTA TGCTATTGGG 
GATATCTTGG ATGGAAAGCT TGAACTTACT CCTGTTGCCA TTCAGGCAGG GAAACTCCTG 
GCCCGCAGGC TCTATGGTGG TAGTTCCACA AAGTGTGACT ATATCAACGT ACCAACGACA 
GTGTTTACTC CTTTAGAGTA CGGCAGCTGT GGGCTAGCTG AAGAGAAAGC CATAGAAGAA 
TATGGAAAAC AAAATTTGGA GGTTTATCAC AGTTTGTTCT GGCCACTTGA ATGGACGGTA 
CCAGGCAGAG ATAACAATAC TTGTTACGCA AAGATTATCT GCAATAAACT AGACGGTAAC 
CGTGTAGTAG GATTTCATGT TCTTGGACCT AATGCTGGTG AAGTTACCCA AGGTTTTGCA 
GCTGCAATAA AGTGTGGTCT CACCAAAGAA CTCCTTGATG AAACAATTGG TATCCATCCA 
ACTTGTGCAG AGGTGTTCAC TACGATGGAT ATTACAAAAT CATCAGGACA AGACATCACT 
CAACGGGGCT GCTGAGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116249.1
CDS	95..1915
Translation	MPPPGQTQLPDWDGLKLRVRTLIATHRVMIFSKSYCPYCHRVKELFSSLGVQYYALELDVTDDGPSIQQVLAELTNQRTVPNVFINGKHIGGCDATYKAYENGTLQRILGDVKDAETYDYDLIVIGGGSGGLACSKEAATLGKKVMVLDYVVPTPLGTSWGLGGTCVNVGCIPKKLMHQAALLGQALKDSRAYGWQYDEQVKHNWEIMVEAVQNYIGSLNWGYRLSLREKSVTYQNSYGEFVEPHKIKATNRKGQVTYHTAETFVLATGERPRYLGIPGDKEYCITSDDLFSLPYCPGKTLVVGASYVALECAGFLAGLGLDVTVMVRSILLRGFDQEMAEKIGAHMETHGVTFIRKFVPTQVERLEDGTPGRLKVTAKSTEGPEFFEGEYNTVLIAIGRDACTRNIGLQTIGVKINEKNGKVPVNDEERTNVPYVYAIGDILDGKLELTPVAIQAGKLLARRLYGGSSTKCDYINVPTTVFTPLEYGSCGLAEEKAIEEYGKQNLEVYHSLFWPLEWTVPGRDNNTCYAKIICNKLDGNRVVGFHVLGPNAGEVTQGFAAAIKCGLTKELLDETIGIHPTCAEVFTTMDITKSSGQDITQRGC*G

Target ORF information:

RefSeq Version	NM_001122777.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thioredoxin reductase 3 (TXNRD3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001122777.2

ORF Insert Sequence:

ATGCCGCCGC CCGGGCAGAC ACAGCTCCCA GACTGGGACG GCCTGAAGCT CCGCGTGCGG 
ACCCTCATCG CCACGCACCG CGTCATGATC TTCAGCAAGA GCTACTGCCC CTACTGCCAT 
CGGGTGAAAG AACTCTTCAG CTCTCTAGGT GTACAGTATT ATGCTTTGGA ACTGGATGTA 
ACTGATGATG GACCCAGTAT TCAGCAAGTG TTAGCAGAGC TAACTAATCA GAGGACGGTT 
CCTAATGTTT TTATCAATGG AAAACATATA GGTGGCTGTG ATGCAACTTA CAAGGCTTAT 
GAGAATGGAA CACTGCAAAG AATTCTTGGG GATGTCAAAG ATGCAGAAAC CTATGATTAT 
GACCTCATTG TTATTGGTGG TGGCTCAGGC GGACTCGCTT GTTCCAAGGA AGCTGCTACC 
TTGGGAAAGA AAGTGATGGT ATTAGATTAT GTTGTGCCAA CACCCCTGGG AACCTCCTGG 
GGACTTGGTG GTACATGTGT AAATGTAGGC TGTATTCCTA AGAAGCTAAT GCATCAAGCG 
GCTCTTCTGG GGCAGGCACT CAAAGATTCA AGGGCATACG GCTGGCAGTA TGATGAACAA 
GTTAAACACA ACTGGGAGAT CATGGTAGAA GCAGTTCAAA ACTACATTGG CTCTTTAAAC 
TGGGGCTATC GATTGTCCTT AAGAGAGAAA TCTGTGACAT ACCAAAATTC CTATGGAGAA 
TTTGTTGAAC CACACAAAAT CAAGGCAACT AATAGAAAAG GACAAGTAAC CTATCACACA 
GCAGAGACTT TTGTCCTGGC AACAGGAGAA AGGCCTAGAT ATCTGGGTAT TCCTGGAGAT 
AAAGAATACT GTATTACAAG TGATGACCTC TTCTCCCTGC CTTACTGCCC TGGCAAAACG 
CTAGTTGTGG GTGCTTCCTA TGTGGCACTG GAGTGCGCAG GATTTCTTGC TGGTTTAGGT 
CTAGATGTCA CAGTAATGGT GCGTTCCATA CTCCTTCGAG GCTTTGACCA AGAGATGGCA 
GAAAAAATAG GTGCCCACAT GGAAACACAT GGTGTAACGT TCATCAGAAA GTTTGTACCT 
ACTCAGGTGG AAAGGCTGGA GGATGGCACA CCGGGAAGAC TAAAAGTGAC AGCAAAGTCT 
ACTGAGGGTC CAGAATTTTT TGAAGGAGAA TATAACACTG TTTTGATAGC CATTGGTCGG 
GATGCATGTA CCAGAAATAT TGGCTTACAG ACAATTGGTG TCAAAATCAA TGAGAAGAAT 
GGGAAAGTAC CTGTAAATGA TGAAGAACGA ACCAATGTGC CTTATGTTTA TGCTATTGGG 
GATATCTTGG ATGGAAAGCT TGAACTTACT CCTGTTGCCA TTCAGGCAGG GAAACTCCTG 
GCCCGCAGGC TCTATGGTGG TAGTTCCACA AAGTGTGACT ATATCAACGT ACCAACGACA 
GTGTTTACTC CTTTAGAGTA CGGCAGCTGT GGGCTAGCTG AAGAGAAAGC CATAGAAGAA 
TATGGAAAAC AAAATTTGGA GGTTTATCAC AGTTTGTTCT GGCCACTTGA ATGGACGGTA 
CCAGGCAGAG ATAACAATAC TTGTTACGCA AAGATTATCT GCAATAAACT AGACGGTAAC 
CGTGTAGTAG GATTTCATGT TCTTGGACCT AATGCTGGTG AAGTTACCCA AGGTTTTGCA 
GCTGCAATAA AGTGTGGTCT CACCAAAGAA CTCCTTGATG AAACAATTGG TATCCATCCA 
ACTTGTGCAG AGGTGTTCAC TACGATGGAT ATTACAAAAT CATCAGGACA AGACATCACT 
CAACGGGGCT GCTGAGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TXNRD3 ( NM_001122777.1 ) cDNA ORF clone, Gallus gallus(Chicken) -> NP_001116249.1 Gallus gallus thioredoxin reductase 3 (TXNRD3), mRNA.

Price & Availability↑

CloneID	OGa26638
Clone ID Related Accession (Same CDS sequence)	NM_001122777.2 , NM_001122777.1
Accession Version	NM_001122777.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-09
Organism	Gallus gallus(Chicken)
Product	thioredoxin reductase 3
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AM071104.1, AADN04000010.1, BU210630.1, AL584253.1, BI067020.1, CF258103.1, CD740317.1 and BU455978.1. On Mar 23, 2008 this sequence version replaced XM_414371.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201358, SAMEA2201361 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCGCCGC CCGGGCAGAC ACAGCTCCCA GACTGGGACG GCCTGAAGCT CCGCGTGCGG 
ACCCTCATCG CCACGCACCG CGTCATGATC TTCAGCAAGA GCTACTGCCC CTACTGCCAT 
CGGGTGAAAG AACTCTTCAG CTCTCTAGGT GTACAGTATT ATGCTTTGGA ACTGGATGTA 
ACTGATGATG GACCCAGTAT TCAGCAAGTG TTAGCAGAGC TAACTAATCA GAGGACGGTT 
CCTAATGTTT TTATCAATGG AAAACATATA GGTGGCTGTG ATGCAACTTA CAAGGCTTAT 
GAGAATGGAA CACTGCAAAG AATTCTTGGG GATGTCAAAG ATGCAGAAAC CTATGATTAT 
GACCTCATTG TTATTGGTGG TGGCTCAGGC GGACTCGCTT GTTCCAAGGA AGCTGCTACC 
TTGGGAAAGA AAGTGATGGT ATTAGATTAT GTTGTGCCAA CACCCCTGGG AACCTCCTGG 
GGACTTGGTG GTACATGTGT AAATGTAGGC TGTATTCCTA AGAAGCTAAT GCATCAAGCG 
GCTCTTCTGG GGCAGGCACT CAAAGATTCA AGGGCATACG GCTGGCAGTA TGATGAACAA 
GTTAAACACA ACTGGGAGAT CATGGTAGAA GCAGTTCAAA ACTACATTGG CTCTTTAAAC 
TGGGGCTATC GATTGTCCTT AAGAGAGAAA TCTGTGACAT ACCAAAATTC CTATGGAGAA 
TTTGTTGAAC CACACAAAAT CAAGGCAACT AATAGAAAAG GACAAGTAAC CTATCACACA 
GCAGAGACTT TTGTCCTGGC AACAGGAGAA AGGCCTAGAT ATCTGGGTAT TCCTGGAGAT 
AAAGAATACT GTATTACAAG TGATGACCTC TTCTCCCTGC CTTACTGCCC TGGCAAAACG 
CTAGTTGTGG GTGCTTCCTA TGTGGCACTG GAGTGCGCAG GATTTCTTGC TGGTTTAGGT 
CTAGATGTCA CAGTAATGGT GCGTTCCATA CTCCTTCGAG GCTTTGACCA AGAGATGGCA 
GAAAAAATAG GTGCCCACAT GGAAACACAT GGTGTAACGT TCATCAGAAA GTTTGTACCT 
ACTCAGGTGG AAAGGCTGGA GGATGGCACA CCGGGAAGAC TAAAAGTGAC AGCAAAGTCT 
ACTGAGGGTC CAGAATTTTT TGAAGGAGAA TATAACACTG TTTTGATAGC CATTGGTCGG 
GATGCATGTA CCAGAAATAT TGGCTTACAG ACAATTGGTG TCAAAATCAA TGAGAAGAAT 
GGGAAAGTAC CTGTAAATGA TGAAGAACGA ACCAATGTGC CTTATGTTTA TGCTATTGGG 
GATATCTTGG ATGGAAAGCT TGAACTTACT CCTGTTGCCA TTCAGGCAGG GAAACTCCTG 
GCCCGCAGGC TCTATGGTGG TAGTTCCACA AAGTGTGACT ATATCAACGT ACCAACGACA 
GTGTTTACTC CTTTAGAGTA CGGCAGCTGT GGGCTAGCTG AAGAGAAAGC CATAGAAGAA 
TATGGAAAAC AAAATTTGGA GGTTTATCAC AGTTTGTTCT GGCCACTTGA ATGGACGGTA 
CCAGGCAGAG ATAACAATAC TTGTTACGCA AAGATTATCT GCAATAAACT AGACGGTAAC 
CGTGTAGTAG GATTTCATGT TCTTGGACCT AATGCTGGTG AAGTTACCCA AGGTTTTGCA 
GCTGCAATAA AGTGTGGTCT CACCAAAGAA CTCCTTGATG AAACAATTGG TATCCATCCA 
ACTTGTGCAG AGGTGTTCAC TACGATGGAT ATTACAAAAT CATCAGGACA AGACATCACT 
CAACGGGGCT GCTGAGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116249.1
CDS	95..1915
Translation	MPPPGQTQLPDWDGLKLRVRTLIATHRVMIFSKSYCPYCHRVKELFSSLGVQYYALELDVTDDGPSIQQVLAELTNQRTVPNVFINGKHIGGCDATYKAYENGTLQRILGDVKDAETYDYDLIVIGGGSGGLACSKEAATLGKKVMVLDYVVPTPLGTSWGLGGTCVNVGCIPKKLMHQAALLGQALKDSRAYGWQYDEQVKHNWEIMVEAVQNYIGSLNWGYRLSLREKSVTYQNSYGEFVEPHKIKATNRKGQVTYHTAETFVLATGERPRYLGIPGDKEYCITSDDLFSLPYCPGKTLVVGASYVALECAGFLAGLGLDVTVMVRSILLRGFDQEMAEKIGAHMETHGVTFIRKFVPTQVERLEDGTPGRLKVTAKSTEGPEFFEGEYNTVLIAIGRDACTRNIGLQTIGVKINEKNGKVPVNDEERTNVPYVYAIGDILDGKLELTPVAIQAGKLLARRLYGGSSTKCDYINVPTTVFTPLEYGSCGLAEEKAIEEYGKQNLEVYHSLFWPLEWTVPGRDNNTCYAKIICNKLDGNRVVGFHVLGPNAGEVTQGFAAAIKCGLTKELLDETIGIHPTCAEVFTTMDITKSSGQDITQRGC*G

Target ORF information:

RefSeq Version	NM_001122777.1
Organism	Gallus gallus(Chicken)
Definition	Gallus gallus thioredoxin reductase 3 (TXNRD3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001122777.1

ORF Insert Sequence:

ATGCCGCCGC CCGGGCAGAC ACAGCTCCCA GACTGGGACG GCCTGAAGCT CCGCGTGCGG 
ACCCTCATCG CCACGCACCG CGTCATGATC TTCAGCAAGA GCTACTGCCC CTACTGCCAT 
CGGGTGAAAG AACTCTTCAG CTCTCTAGGT GTACAGTATT ATGCTTTGGA ACTGGATGTA 
ACTGATGATG GACCCAGTAT TCAGCAAGTG TTAGCAGAGC TAACTAATCA GAGGACGGTT 
CCTAATGTTT TTATCAATGG AAAACATATA GGTGGCTGTG ATGCAACTTA CAAGGCTTAT 
GAGAATGGAA CACTGCAAAG AATTCTTGGG GATGTCAAAG ATGCAGAAAC CTATGATTAT 
GACCTCATTG TTATTGGTGG TGGCTCAGGC GGACTCGCTT GTTCCAAGGA AGCTGCTACC 
TTGGGAAAGA AAGTGATGGT ATTAGATTAT GTTGTGCCAA CACCCCTGGG AACCTCCTGG 
GGACTTGGTG GTACATGTGT AAATGTAGGC TGTATTCCTA AGAAGCTAAT GCATCAAGCG 
GCTCTTCTGG GGCAGGCACT CAAAGATTCA AGGGCATACG GCTGGCAGTA TGATGAACAA 
GTTAAACACA ACTGGGAGAT CATGGTAGAA GCAGTTCAAA ACTACATTGG CTCTTTAAAC 
TGGGGCTATC GATTGTCCTT AAGAGAGAAA TCTGTGACAT ACCAAAATTC CTATGGAGAA 
TTTGTTGAAC CACACAAAAT CAAGGCAACT AATAGAAAAG GACAAGTAAC CTATCACACA 
GCAGAGACTT TTGTCCTGGC AACAGGAGAA AGGCCTAGAT ATCTGGGTAT TCCTGGAGAT 
AAAGAATACT GTATTACAAG TGATGACCTC TTCTCCCTGC CTTACTGCCC TGGCAAAACG 
CTAGTTGTGG GTGCTTCCTA TGTGGCACTG GAGTGCGCAG GATTTCTTGC TGGTTTAGGT 
CTAGATGTCA CAGTAATGGT GCGTTCCATA CTCCTTCGAG GCTTTGACCA AGAGATGGCA 
GAAAAAATAG GTGCCCACAT GGAAACACAT GGTGTAACGT TCATCAGAAA GTTTGTACCT 
ACTCAGGTGG AAAGGCTGGA GGATGGCACA CCGGGAAGAC TAAAAGTGAC AGCAAAGTCT 
ACTGAGGGTC CAGAATTTTT TGAAGGAGAA TATAACACTG TTTTGATAGC CATTGGTCGG 
GATGCATGTA CCAGAAATAT TGGCTTACAG ACAATTGGTG TCAAAATCAA TGAGAAGAAT 
GGGAAAGTAC CTGTAAATGA TGAAGAACGA ACCAATGTGC CTTATGTTTA TGCTATTGGG 
GATATCTTGG ATGGAAAGCT TGAACTTACT CCTGTTGCCA TTCAGGCAGG GAAACTCCTG 
GCCCGCAGGC TCTATGGTGG TAGTTCCACA AAGTGTGACT ATATCAACGT ACCAACGACA 
GTGTTTACTC CTTTAGAGTA CGGCAGCTGT GGGCTAGCTG AAGAGAAAGC CATAGAAGAA 
TATGGAAAAC AAAATTTGGA GGTTTATCAC AGTTTGTTCT GGCCACTTGA ATGGACGGTA 
CCAGGCAGAG ATAACAATAC TTGTTACGCA AAGATTATCT GCAATAAACT AGACGGTAAC 
CGTGTAGTAG GATTTCATGT TCTTGGACCT AATGCTGGTG AAGTTACCCA AGGTTTTGCA 
GCTGCAATAA AGTGTGGTCT CACCAAAGAA CTCCTTGATG AAACAATTGG TATCCATCCA 
ACTTGTGCAG AGGTGTTCAC TACGATGGAT ATTACAAAAT CATCAGGACA AGACATCACT 
CAACGGGGCT GCTGAGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selenoprotein Gene Nomenclature.
The Journal of biological chemistry291(46)24036-24040(2016 Nov)
Gladyshev VN,Arn?r ES,Berry MJ,Brigelius-Floh? R,Bruford EA,Burk RF,Carlson BA,Castellano S,Chavatte L,Conrad M,Copeland PR,Diamond AM,Driscoll DM,Ferreiro A,Floh? L,Green FR,Guig? R,Handy DE,Hatfield DL,Hesketh J,Hoffmann PR,Holmgren A,Hondal RJ,Howard MT,Huang K,Kim HY,Kim IY,K?hrle J,Krol A,Kryukov GV,Lee BJ,Lee BC,Lei XG,Liu Q,Lescure A,Lobanov AV,Loscalzo J,Maiorino M,Mariotti M,Sandeep Prabhu K,Rayman MP,Rozovsky S,Salinas G,Schmidt EE,Schomburg L,Schweizer U,Simonovi? M,Sunde RA,Tsuji PA,Tweedie S,Ursini F,Whanger PD,Zhang Y

Dietary Selenium Status Regulates the Transcriptions of Selenoproteome and Activities of Selenoenzymes in Chicken Kidney at Low or Super-nutritional Levels.
Biological trace element research170(2)438-48(2016 Apr)
Xu JX,Zhang C,Cao CY,Zhu SY,Li H,Sun YC,Li JL

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

Non-coding RNAs revealed during identification of genes involved in chicken immune responses.
Immunogenetics61(1)55-70(2009 Jan)
Ahanda ML,Ruby T,Wittzell H,Bed'Hom B,Chauss? AM,Morin V,Oudin A,Chevalier C,Young JR,Zoorob R

Gene expression profiling of chicken primordial germ cell ESTs.
BMC genomics7220(2006 Aug)
Han JY,Park TS,Kim JN,Kim MA,Lim D,Lim JM,Kim H

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TXNRD3 cDNA ORF clone, Gallus gallus(Chicken)

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