SPG11 cDNA ORF clone, Gallus gallus(chicken)

The following SPG11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPG11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa143468 XM_025153966.1
Latest version!
Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OGa143469 XM_025153967.1
Latest version!
Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa13353 XM_003641831.4
Latest version!
Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OGa13354 XM_004943936.3
Latest version!
Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OGa13355 XM_004943937.3
Latest version!
Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OGa13357 XM_004943939.3
Latest version!
Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
View Old Accession Versions >>
OGa13357 XM_004943939.2 Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OGa13354 XM_004943936.2 Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OGa13353 XM_003641831.3 Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OGa13355 XM_004943937.2 Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa143468
    Clone ID Related Accession (Same CDS sequence) XM_025153966.1
    Accession Version XM_025153966.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7176bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product spatacsin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
    GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC
    CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG
    CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT
    GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC
    TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG
    GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG
    AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG
    CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT
    TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT
    TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA
    CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC
    ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT
    TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT
    CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG
    GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC
    AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT
    TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA
    GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT
    TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG
    CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT
    AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA
    CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC
    GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC
    TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC
    AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA
    GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA
    AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA
    GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG
    CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG
    CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA
    GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA
    CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT
    GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT
    GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC
    CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG
    GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG
    GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA
    CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA
    ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA
    GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT
    TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC
    AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC
    CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG
    ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC
    AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG
    TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT
    GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA
    GTACTGCAAA ATCCTCACCC TGTTCCAAAT TTAACTCCTT CCAACTGCCC TATTTTGGAT
    GACAAGGAAC TGCATGAAGC ACATCCCTGG TTTGAATTCC TCGTGCAGTG CCGGGGGGTT
    GCCAGTAACC CACGAGATCC AAAGATGATT TTTCAGGCGA GTCTAGCAAA TGCTCAGATC
    TTGATTCCTA GTAATCAAGC CAGCGTGAGT AGCATGCTGC TGGAGGGACG TACACTGTTA
    GCACTTGCTA CCACCATGTA TGCACCTGGA GGCATTGATC AGGTTCTTCA GAATGAAGAT
    ACAGAGAAAC CACTAAAGAA AGTTGATCCT CAGCTCTTAA AAATGGCATT GAGTCCTTAC
    CCCAAACTCA GAGCTGCTCT TTTTCCCCCG TGCACCTCCC ATGGAATTTT GCCACCTGAC
    ATTTCCCTTT ATCATCTTGT TCAGTCATTA GTGCCGTTTG ATCCCAGTAA GTTATTTGGC
    TGGCAGTCAA CAAATACCCT TGCTGTATCA GATGCATCAA GTGACCTTCC TCATTTCTCA
    TCCCCTGAAC TGGTGAACAA ATATGCTGTA ATGGAACGAC TGGACTTTTT GTACTACCTG
    CGCCATGGAC GCCCATCGTT TGCCTTTGGT ACATTTTTGG TCCAGCAGTT GGTCAAGAGC
    AAAAGCCCCA AACAGTTGAT TCAGCAAGCA GGAAATGAAG CCTATCTTCT AGGCCTTTCT
    TTCTTCAATA CGCCTTCAGT AGCAGCTGCC TGTGTGTGCT TTCTGGAGCT GCTTGGGCTC
    AGCAGCCTCA AACTGAGAGT TGATATTAAG GTTGCAAACA TGATATTCAT TTACAAAACC
    AGAAATGAAG AAACTCAGCA CAATCAAATA AGGGAATCTC TGGTTGAACA GCTAATGAGG
    TTGGCTGATG GTGAAAAAGC AGCAACAGAA GAAGTTCTTA AATCCTTAGA AGAAGCCTTT
    TGGGATTCAA TTGAGCATCA AGGAATAAAG AGGACATCCA GTGACTCCAG ACAGCAGTGG
    TCTTTGGTGA TGCAGTTCTG CAAACTCCAT GCCATGAAAC TGAGCACCTC ATACCTAAAG
    GAGTGTGCTA AATCCAATGA CTGGCTGCAG TTCATCATCC AAGCCCAGCT GTACAGCTAC
    AAGCCAGATG AGGTCTCATC TGTCCTTGAA GGCTTCGCTC CCATTATACA GCGTCACCTG
    ATGTTGGCAC TGGAGAAGTC ACCGTTTCTG CACGCTGCTG CGGGGGCTCC TGAGGGCAGC
    ACTGGGAGCA GATGTGCAGA CAGTCTCTTC CAAATCCTTT TCCGGTGTCA GGAGAGCCCC
    AATGCTTCGT GTCACCTTTT GGCTGAAGGA CTGAGAGAAC ATGCTCCCAT TCTGAGTGTT
    TTAGCAGCGT GTTCCCCTGA TGCTGACATT ATTCACTGTC TCTGTGTCTG GATAATTACC
    AGTGTGGATG ATGTTACCAG ATCTGAAGCG ACAAAACATA TTCAGAGCTC AGTAGAAAAT
    CACAAGTGGG ATCTTCATGA CCTGTCGCTT ATCTGGAAAG TGCTTTTAAG AAAGCAAAAA
    TGTCAAACAC TTATAAGGGG ATTCCAGCTC TTCTTAAAGG ATTCCCCTTT ACTGCATATA
    CTGGAGATGT ACGATCTGTG CATGAACTGT AAAAGGTACT ATGAAGCCAA AACCAAACTG
    GACGAGTTTC AGAAACACCT CACAAATCTG AAGACCACCG ATGGAGCGGC AGCTTCAGGA
    GTCCCTGTGC AATGGTTGGA GTCACAGGCC TTGTTTCTTC TTGAACTGAT GATGCAGCAG
    TGTAGGACAG AATATGAATT GGGAAAGCTC TTACAGCTGT TTGCTGCAAC AGACAACCTT
    CTCCTTGATG GCCCTTATGT GAAGAAACTG TGTGCGCTCA GTGAGACCCT GAGGGATTCC
    TCCGTGCCCA TTAATCGCTC CATGCTGACC AGGTACACCC CAGAAACGTT TCAGAAGGAG
    TGCACATCGA TTCTGGAGCA GCTCCAGGAG AAAGGAATGT TCTCTCTGGC TCGGGAGGTG
    GCAGAGCTGG CTGAACTGCC TGCGGACAGC GTGGTTACCC AAGAGATTCT GAGGGATATA
    CGTCTGTTAA GAGACACAGG AGAGTGGGAT CGAGATCAGA TAAGAATTAA GTTCTGGAAA
    AAGTGTAACG ACAGCTTCGT GAAAAATTCC ATCTCCAGCA AAGCTGCATC GGATTTCTTT
    TTGAATCAGG GCAATGTGGT GTCTGAATCC CCAGTTCGTG AGAAGATAAA CAGCATTTCG
    GAGAGACATC TGCTGCTTAC CCTGGCTGGC CATTGGCTCG CCAAGGATGT CTCGGTGGCA
    TTGGACAAAT TGGAGGAAAT AGAGAAACAG ATCTGGATTT GTCGCATCAC ACAACAAATG
    TTATCCACAG ATGAGGGGCC AGGCAAGTCG GGACGCTCCT CCTGTGCTGC AGCGAATGGG
    GATTTTACTT TTGATAACTT GGCCAAGGAG TTTTCTTTTT CAAAGCTGCC TGCTCTGAAC
    ACACCAAACT ACTTAAACGT GGAGGGTCTT GAATTGACGG GGTGTCAGCA GACCTTGGCT
    GCGGAGGAGG AGGAGTCGCT CAGGTTTCTG GTCGGATGCC TTCTCGATGA AGGAAGCGTG
    CACGAGGCCA GCCGCGTTTG TCGCTATTTC CATTTCTACA GCAGAGACGT TTCACTGGTG
    TTGCACTGCA GAGCCCTGGC CGCAGGGGAG GCTGACCTGG ACAGACTGCA CGCAGATATT
    CAGGCTCTTG TAGCAATAGA GAAGACACAG GACAGCAGTG CATCGCAGCA GAGCAGAGTC
    CCGAGCACAT CGAGCTTAGA GGACTGGACA CACATTGCAG ATCCATCGCC GGATGATCAG
    ATTGTCGTCA GTCTGAAGGC CCTCACAGAT GAATGTGTCC ATGGGAGAAA CTACTGCAGA
    CAGGTTCTGT GTCTGTATGA ACTTTCTAAG GAACTGAACT GCTCCTACAG TGAGATGTCA
    GCCCTTGACC CTGAGAAAGT GCTTCGAGCA ATTCTGTCAT CCCAGCAGGC AGAGCGCTGC
    AGGAGGGCTC AGGCGTTCAT CACCACGCAG GGCCTGCAGC CCGAAACTGT GGCAGAGCTG
    GTGGCCAAAG AGGTCGTGCA GGAGTTACTG GCCTCTTCAG AAGGAAAAGG ACAGAAGCAG
    GTTTCCAACC CTGCAGCTGA GAGCCAGGCG TTCCTGCAGC TCGCAAAGCT GTGCCAGGAC
    CACACGCTGG TGGGCATGAA GCTGCTGGAG AAAATTTCCT CTGTGCCACG TGGAGAACTG
    TCATGCATTA CAGAACTGCT GATTCTGGCC CATAATTGCT TTAGTCTGAC TTGCCACATG
    GAAGGAATCA CCAGAGTCCT GCAGGCAGCA CGGCTGCTCA CAGATGAGCA TTTGGCACCT
    AATGATGAGT ATGGACTTAT GGTTCGTCTT CTCACTGGGA TCGGCAGATA CAATGAGATG
    ACCTACATCT TTGAGCTGCT GCACGAGAAG CATTACTTTG AAGTGCTCAT GAGGAAGAAG
    TTGGACCCGA GTGGGACACT GAAGACAGCT CTGCTGGATT ACATCAAGCG CTGCCGCCCA
    GGGGACAGTG AGAAGCACAA CATGATTGCC CTTTGCTTCA GCATGTGCCG GGAAATTGGA
    GAGAATCACG AGGCTGCTGC TTGCATACAG CTGAAACTCA TTGAATCTCA GCCATGGGAG
    GAGTCCCTTC AGGATGTTGC AAACTTAAAG AAACTGCTGA TGAAAGCTCT GACCCTCTTC
    ATTGATGCAG CAGAAAGTTA CTCTAAGGAC TCCTGTGTCC GCCAGGCGCT GCGCTGCCGC
    CGCCTCACAA AGCTGATCAC TCTGCAGCTG CATTTTCTGA GCACCGGTCA GAGCACGATG
    CTGATTAACA GGAACAGGCA GAACCTCATG GAATGCATCG TGTCCCTGCC TCGATTCTAT
    CAGGCGGCGG TTGTGGCCGA GGCATATGAG TTTGTTCCAG ATTGGGCTGA AGTTCTGTAC
    CAACAAGTGA TCGCTAAGGG AGACTTCAGC TACTTAGAAG AATTTAAACA GCAGAGACTG
    CTGAAGCCTG CCATGTTTGA GGAGATTGCG AAGAAGTGTA AGCAGCACCC ACCCAGCGAT
    GCTGCTCTGA GGAATCTGAA GAAGCTGCTC GCTTACTGCG AGGACATCTC CACGTACTAC
    AGGCTGGCAT ATGATAACAA GTTCTATGAT GTGGTCAATA TGCTCCTGAA GGACGCGCAG
    ACTGGATGCT GCCTTAATGA TATGTTATCC AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009734.1
    CDS143..7318
    Translation

    Target ORF information:

    RefSeq Version XM_025153966.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153966.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
    GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC
    CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG
    CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT
    GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC
    TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG
    GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG
    AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG
    CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT
    TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT
    TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA
    CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC
    ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT
    TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT
    CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG
    GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC
    AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT
    TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA
    GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT
    TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG
    CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT
    AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA
    CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC
    GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC
    TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC
    AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA
    GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA
    AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA
    GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG
    CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG
    CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA
    GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA
    CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT
    GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT
    GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC
    CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG
    GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG
    GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA
    CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA
    ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA
    GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT
    TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC
    AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC
    CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG
    ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC
    AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG
    TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT
    GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA
    GTACTGCAAA ATCCTCACCC TGTTCCAAAT TTAACTCCTT CCAACTGCCC TATTTTGGAT
    GACAAGGAAC TGCATGAAGC ACATCCCTGG TTTGAATTCC TCGTGCAGTG CCGGGGGGTT
    GCCAGTAACC CACGAGATCC AAAGATGATT TTTCAGGCGA GTCTAGCAAA TGCTCAGATC
    TTGATTCCTA GTAATCAAGC CAGCGTGAGT AGCATGCTGC TGGAGGGACG TACACTGTTA
    GCACTTGCTA CCACCATGTA TGCACCTGGA GGCATTGATC AGGTTCTTCA GAATGAAGAT
    ACAGAGAAAC CACTAAAGAA AGTTGATCCT CAGCTCTTAA AAATGGCATT GAGTCCTTAC
    CCCAAACTCA GAGCTGCTCT TTTTCCCCCG TGCACCTCCC ATGGAATTTT GCCACCTGAC
    ATTTCCCTTT ATCATCTTGT TCAGTCATTA GTGCCGTTTG ATCCCAGTAA GTTATTTGGC
    TGGCAGTCAA CAAATACCCT TGCTGTATCA GATGCATCAA GTGACCTTCC TCATTTCTCA
    TCCCCTGAAC TGGTGAACAA ATATGCTGTA ATGGAACGAC TGGACTTTTT GTACTACCTG
    CGCCATGGAC GCCCATCGTT TGCCTTTGGT ACATTTTTGG TCCAGCAGTT GGTCAAGAGC
    AAAAGCCCCA AACAGTTGAT TCAGCAAGCA GGAAATGAAG CCTATCTTCT AGGCCTTTCT
    TTCTTCAATA CGCCTTCAGT AGCAGCTGCC TGTGTGTGCT TTCTGGAGCT GCTTGGGCTC
    AGCAGCCTCA AACTGAGAGT TGATATTAAG GTTGCAAACA TGATATTCAT TTACAAAACC
    AGAAATGAAG AAACTCAGCA CAATCAAATA AGGGAATCTC TGGTTGAACA GCTAATGAGG
    TTGGCTGATG GTGAAAAAGC AGCAACAGAA GAAGTTCTTA AATCCTTAGA AGAAGCCTTT
    TGGGATTCAA TTGAGCATCA AGGAATAAAG AGGACATCCA GTGACTCCAG ACAGCAGTGG
    TCTTTGGTGA TGCAGTTCTG CAAACTCCAT GCCATGAAAC TGAGCACCTC ATACCTAAAG
    GAGTGTGCTA AATCCAATGA CTGGCTGCAG TTCATCATCC AAGCCCAGCT GTACAGCTAC
    AAGCCAGATG AGGTCTCATC TGTCCTTGAA GGCTTCGCTC CCATTATACA GCGTCACCTG
    ATGTTGGCAC TGGAGAAGTC ACCGTTTCTG CACGCTGCTG CGGGGGCTCC TGAGGGCAGC
    ACTGGGAGCA GATGTGCAGA CAGTCTCTTC CAAATCCTTT TCCGGTGTCA GGAGAGCCCC
    AATGCTTCGT GTCACCTTTT GGCTGAAGGA CTGAGAGAAC ATGCTCCCAT TCTGAGTGTT
    TTAGCAGCGT GTTCCCCTGA TGCTGACATT ATTCACTGTC TCTGTGTCTG GATAATTACC
    AGTGTGGATG ATGTTACCAG ATCTGAAGCG ACAAAACATA TTCAGAGCTC AGTAGAAAAT
    CACAAGTGGG ATCTTCATGA CCTGTCGCTT ATCTGGAAAG TGCTTTTAAG AAAGCAAAAA
    TGTCAAACAC TTATAAGGGG ATTCCAGCTC TTCTTAAAGG ATTCCCCTTT ACTGCATATA
    CTGGAGATGT ACGATCTGTG CATGAACTGT AAAAGGTACT ATGAAGCCAA AACCAAACTG
    GACGAGTTTC AGAAACACCT CACAAATCTG AAGACCACCG ATGGAGCGGC AGCTTCAGGA
    GTCCCTGTGC AATGGTTGGA GTCACAGGCC TTGTTTCTTC TTGAACTGAT GATGCAGCAG
    TGTAGGACAG AATATGAATT GGGAAAGCTC TTACAGCTGT TTGCTGCAAC AGACAACCTT
    CTCCTTGATG GCCCTTATGT GAAGAAACTG TGTGCGCTCA GTGAGACCCT GAGGGATTCC
    TCCGTGCCCA TTAATCGCTC CATGCTGACC AGGTACACCC CAGAAACGTT TCAGAAGGAG
    TGCACATCGA TTCTGGAGCA GCTCCAGGAG AAAGGAATGT TCTCTCTGGC TCGGGAGGTG
    GCAGAGCTGG CTGAACTGCC TGCGGACAGC GTGGTTACCC AAGAGATTCT GAGGGATATA
    CGTCTGTTAA GAGACACAGG AGAGTGGGAT CGAGATCAGA TAAGAATTAA GTTCTGGAAA
    AAGTGTAACG ACAGCTTCGT GAAAAATTCC ATCTCCAGCA AAGCTGCATC GGATTTCTTT
    TTGAATCAGG GCAATGTGGT GTCTGAATCC CCAGTTCGTG AGAAGATAAA CAGCATTTCG
    GAGAGACATC TGCTGCTTAC CCTGGCTGGC CATTGGCTCG CCAAGGATGT CTCGGTGGCA
    TTGGACAAAT TGGAGGAAAT AGAGAAACAG ATCTGGATTT GTCGCATCAC ACAACAAATG
    TTATCCACAG ATGAGGGGCC AGGCAAGTCG GGACGCTCCT CCTGTGCTGC AGCGAATGGG
    GATTTTACTT TTGATAACTT GGCCAAGGAG TTTTCTTTTT CAAAGCTGCC TGCTCTGAAC
    ACACCAAACT ACTTAAACGT GGAGGGTCTT GAATTGACGG GGTGTCAGCA GACCTTGGCT
    GCGGAGGAGG AGGAGTCGCT CAGGTTTCTG GTCGGATGCC TTCTCGATGA AGGAAGCGTG
    CACGAGGCCA GCCGCGTTTG TCGCTATTTC CATTTCTACA GCAGAGACGT TTCACTGGTG
    TTGCACTGCA GAGCCCTGGC CGCAGGGGAG GCTGACCTGG ACAGACTGCA CGCAGATATT
    CAGGCTCTTG TAGCAATAGA GAAGACACAG GACAGCAGTG CATCGCAGCA GAGCAGAGTC
    CCGAGCACAT CGAGCTTAGA GGACTGGACA CACATTGCAG ATCCATCGCC GGATGATCAG
    ATTGTCGTCA GTCTGAAGGC CCTCACAGAT GAATGTGTCC ATGGGAGAAA CTACTGCAGA
    CAGGTTCTGT GTCTGTATGA ACTTTCTAAG GAACTGAACT GCTCCTACAG TGAGATGTCA
    GCCCTTGACC CTGAGAAAGT GCTTCGAGCA ATTCTGTCAT CCCAGCAGGC AGAGCGCTGC
    AGGAGGGCTC AGGCGTTCAT CACCACGCAG GGCCTGCAGC CCGAAACTGT GGCAGAGCTG
    GTGGCCAAAG AGGTCGTGCA GGAGTTACTG GCCTCTTCAG AAGGAAAAGG ACAGAAGCAG
    GTTTCCAACC CTGCAGCTGA GAGCCAGGCG TTCCTGCAGC TCGCAAAGCT GTGCCAGGAC
    CACACGCTGG TGGGCATGAA GCTGCTGGAG AAAATTTCCT CTGTGCCACG TGGAGAACTG
    TCATGCATTA CAGAACTGCT GATTCTGGCC CATAATTGCT TTAGTCTGAC TTGCCACATG
    GAAGGAATCA CCAGAGTCCT GCAGGCAGCA CGGCTGCTCA CAGATGAGCA TTTGGCACCT
    AATGATGAGT ATGGACTTAT GGTTCGTCTT CTCACTGGGA TCGGCAGATA CAATGAGATG
    ACCTACATCT TTGAGCTGCT GCACGAGAAG CATTACTTTG AAGTGCTCAT GAGGAAGAAG
    TTGGACCCGA GTGGGACACT GAAGACAGCT CTGCTGGATT ACATCAAGCG CTGCCGCCCA
    GGGGACAGTG AGAAGCACAA CATGATTGCC CTTTGCTTCA GCATGTGCCG GGAAATTGGA
    GAGAATCACG AGGCTGCTGC TTGCATACAG CTGAAACTCA TTGAATCTCA GCCATGGGAG
    GAGTCCCTTC AGGATGTTGC AAACTTAAAG AAACTGCTGA TGAAAGCTCT GACCCTCTTC
    ATTGATGCAG CAGAAAGTTA CTCTAAGGAC TCCTGTGTCC GCCAGGCGCT GCGCTGCCGC
    CGCCTCACAA AGCTGATCAC TCTGCAGCTG CATTTTCTGA GCACCGGTCA GAGCACGATG
    CTGATTAACA GGAACAGGCA GAACCTCATG GAATGCATCG TGTCCCTGCC TCGATTCTAT
    CAGGCGGCGG TTGTGGCCGA GGCATATGAG TTTGTTCCAG ATTGGGCTGA AGTTCTGTAC
    CAACAAGTGA TCGCTAAGGG AGACTTCAGC TACTTAGAAG AATTTAAACA GCAGAGACTG
    CTGAAGCCTG CCATGTTTGA GGAGATTGCG AAGAAGTGTA AGCAGCACCC ACCCAGCGAT
    GCTGCTCTGA GGAATCTGAA GAAGCTGCTC GCTTACTGCG AGGACATCTC CACGTACTAC
    AGGCTGGCAT ATGATAACAA GTTCTATGAT GTGGTCAATA TGCTCCTGAA GGACGCGCAG
    ACTGGATGCT GCCTTAATGA TATGTTATCC AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa143469
    Clone ID Related Accession (Same CDS sequence) XM_025153967.1
    Accession Version XM_025153967.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6351bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product spatacsin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGAGCTGC TGTCCTCGCC TTTCCGCACA GACAGGTCAT GGAAGGCACA CTTATCCTCC 
    CTGTGCAGCA CAATAAGGAA AAGAAGATCA CGTTCCCCAC ATTTGCTATG TGATATGAAC
    TTACCTTGGT ATCAGTATTT TACCCACCTT GAAGATCACG ATCCCTTAAT CTATGAAACC
    TCTGAGAAGG CACTGGCCAT TGCTCCAAAG GCTTTTCCAC GTGTCCTTTC CAACCCATCG
    CAGAGTGCTG GCACAAAGCT CAGTGATATG GGGCAGCAGT GGAAACAAAT CCTCCCCGTA
    CAGTTATCAC AAGAAGTGGT GAAACTGGAG TGTAAATCTG TGACTGGAGC CAAAGCACTG
    TTTGTGATCC TAACAGATGA CAAGGCTTTG ACACTTGCTC TCTGGGATTT CGAGTCCCAG
    GATGTAGCAC ATTACCAGTT TGGCAAAAAC AGCGTTTATG TGGATTGTAA TGAGGAGGTG
    CCATTATGCC TGCTGCTGAC AGAGTGTGGC CTCTCCTTAA TTCTGTTTGG TTTAACTCAA
    GAGGAGTTTT TGAATAGACT GATGATCTAT GGCAGCGCTG GAGTCGTAGA CTCTGTATGT
    CACCTCAATG GATGGGGACG GTGTTCAATT CCAGTACATG CATTGGAGGC TGGCTTGGAA
    AATCGTCAGC TAGACACAGT GGACTTATTT TTAAAAAGCA AAGAAAGTCT TTTCAATCTC
    TCTGCGCTGC GCTCCGAAGC CCAGCAGTCT GCAGATGGTG CATCCTGCTC TTTTCTGAAA
    AATTTGCAAG AGCTGAGACC AGCTTTAAAC TTGCTTTGCT CAGCAATCAG AGAGAACGAC
    ATGGAGCCGC AGAGCAAACA CTTCTCAGAG CAGCTGCTGA ACCTCACGCT GACTTTCCTT
    AGCAAGCAGC TTGAAGAAAT TTTTGTCCAC ACAGAGGAAC CTGATAGATA CCTGCAGAAG
    GCTGCAGAGA TTTTAACTGA TTACATCATT GAACTTAGGA CGTTTATGAA AAACTATCCT
    CGATCACAAG TAAATACCAC AAATGTAGGG AGTGATTCTG ATGAAGACCT GCCTGCAATA
    GAGGAAAGCC AGATGTGGGA AAAACTGACA CCTGAGGAAG TCATTGCTGA AGCCATCTTA
    AGCAACAAAA TGCCAGAGGC CCAGATCTTC TTCCGAATGC ACCGGCATCC TGCTCAGAGC
    CTTCAGGAAC TCATTCGGAC AGGTTTGGAT CTGGTCTATG GCCATCTTCT GAAGGGCAAT
    ACCAGAGAGG CCTCAGCACT TCTGAAGAAC ATGGGTTTTG ATGTGAAGCA GCAGTTGCAT
    AAGATATGTT TCTACACGGC TGATAAACAC GTACGAGATT TGCTGGTCAA AGTTTTACAA
    GAAGAAAATT ATTTTTCTGA AAAAGAGAAG AAAATGATAG ACTTTGTGCA TCAGGTTGAA
    AGCTTGTACT CGGAGTCCTT TCAAGAAAAT AAAGAGATGC AATCTCTTTC CAGTGTAAGG
    AACTGGAGAC AGGAACAAGA CTCTTCCAAA CATTTGGCCA CTCTGGAGTC ATTGATGGAT
    CATGATGGAC GCCGGGTGCC CAGCAGGGAG GTCAGGGTGA TGCTGAACTG GGCTCAGTGC
    TGGGATGAGC TCCTTCAGGA TATGATTCTT CTCCCCAGAA AACAAAGCCA AGGACTTGCC
    ACATGTAACC CTGAGGCTCT TTGGATGTAC CTGACAGCCC AGCATGATTG GCTTAATATC
    AGTTCATGGA TTGAAGAATT TGAGCCCAAC CACAGCTTAT GTGGACAAAG TAAATGGCCA
    CCCCTGGCTG CAAGCATTGT TGACCAACAC ACGTTGTGTA GTGGCTACAT GAGAAATGAA
    ATATTGAACA AGCTGGCCAG AAATGGGATT TTTGTCCTTT CTGAATTGGA AGACTTTGAA
    CTTCTGCTCC AAAGATTGGT TTCCCTTGGA GGAGTACTGC AAAATCCTCA CCCTGTTCCA
    AAGTACAGTG CTGCGAGTGG TTTGGACTTC CATGCTCGTT TTGTCCTTCA CTGCTTAGAA
    CACAATTTGC AGTATATGTT ATACACATAC CTAGAATACT ACAGTTTAAC TCCTTCCAAC
    TGCCCTATTT TGGATGACAA GGAACTGCAT GAAGCACATC CCTGGTTTGA ATTCCTCGTG
    CAGTGCCGGG GGGTTGCCAG TAACCCACGA GATCCAAAGA TGATTTTTCA GGCGAGTCTA
    GCAAATGCTC AGATCTTGAT TCCTAGTAAT CAAGCCAGCG TGAGTAGCAT GCTGCTGGAG
    GGACGTACAC TGTTAGCACT TGCTACCACC ATGTATGCAC CTGGAGGCAT TGATCAGGTT
    CTTCAGAATG AAGATACAGA GAAACCACTA AAGAAAGTTG ATCCTCAGCT CTTAAAAATG
    GCATTGAGTC CTTACCCCAA ACTCAGAGCT GCTCTTTTTC CCCCGTGCAC CTCCCATGGA
    ATTTTGCCAC CTGACATTTC CCTTTATCAT CTTGTTCAGT CATTAGTGCC GTTTGATCCC
    AGTAAGTTAT TTGGCTGGCA GTCAACAAAT ACCCTTGCTG TATCAGATGC ATCAAGTGAC
    CTTCCTCATT TCTCATCCCC TGAACTGGTG AACAAATATG CTGTAATGGA ACGACTGGAC
    TTTTTGTACT ACCTGCGCCA TGGACGCCCA TCGTTTGCCT TTGGTACATT TTTGGTCCAG
    CAGTTGGTCA AGAGCAAAAG CCCCAAACAG TTGATTCAGC AAGCAGGAAA TGAAGCCTAT
    CTTCTAGGCC TTTCTTTCTT CAATACGCCT TCAGTAGCAG CTGCCTGTGT GTGCTTTCTG
    GAGCTGCTTG GGCTCAGCAG CCTCAAACTG AGAGTTGATA TTAAGGTTGC AAACATGATA
    TTCATTTACA AAACCAGAAA TGAAGAAACT CAGCACAATC AAATAAGGGA ATCTCTGGTT
    GAACAGCTAA TGAGGTTGGC TGATGGTGAA AAAGCAGCAA CAGAAGAAGT TCTTAAATCC
    TTAGAAGAAG CCTTTTGGGA TTCAATTGAG CATCAAGGAA TAAAGAGGAC ATCCAGTGAC
    TCCAGACAGC AGTGGTCTTT GGTGATGCAG TTCTGCAAAC TCCATGCCAT GAAACTGAGC
    ACCTCATACC TAAAGGAGTG TGCTAAATCC AATGACTGGC TGCAGTTCAT CATCCAAGCC
    CAGCTGTACA GCTACAAGCC AGATGAGGTC TCATCTGTCC TTGAAGGCTT CGCTCCCATT
    ATACAGCGTC ACCTGATGTT GGCACTGGAG AAGTCACCGT TTCTGCACGC TGCTGCGGGG
    GCTCCTGAGG GCAGCACTGG GAGCAGATGT GCAGACAGTC TCTTCCAAAT CCTTTTCCGG
    TGTCAGGAGA GCCCCAATGC TTCGTGTCAC CTTTTGGCTG AAGGACTGAG AGAACATGCT
    CCCATTCTGA GTGTTTTAGC AGCGTGTTCC CCTGATGCTG ACATTATTCA CTGTCTCTGT
    GTCTGGATAA TTACCAGTGT GGATGATGTT ACCAGATCTG AAGCGACAAA ACATATTCAG
    AGCTCAGTAG AAAATCACAA GTGGGATCTT CATGACCTGT CGCTTATCTG GAAAGTGCTT
    TTAAGAAAGC AAAAATGTCA AACACTTATA AGGGGATTCC AGCTCTTCTT AAAGGATTCC
    CCTTTACTGC ATATACTGGA GATGTACGAT CTGTGCATGA ACTGTAAAAG GTACTATGAA
    GCCAAAACCA AACTGGACGA GTTTCAGAAA CACCTCACAA ATCTGAAGAC CACCGATGGA
    GCGGCAGCTT CAGGAGTCCC TGTGCAATGG TTGGAGTCAC AGGCCTTGTT TCTTCTTGAA
    CTGATGATGC AGCAGTGTAG GACAGAATAT GAATTGGGAA AGCTCTTACA GCTGTTTGCT
    GCAACAGACA ACCTTCTCCT TGATGGCCCT TATGTGAAGA AACTGTGTGC GCTCAGTGAG
    ACCCTGAGGG ATTCCTCCGT GCCCATTAAT CGCTCCATGC TGACCAGGTA CACCCCAGAA
    ACGTTTCAGA AGGAGTGCAC ATCGATTCTG GAGCAGCTCC AGGAGAAAGG AATGTTCTCT
    CTGGCTCGGG AGGTGGCAGA GCTGGCTGAA CTGCCTGCGG ACAGCGTGGT TACCCAAGAG
    ATTCTGAGGG ATATACGTCT GTTAAGAGAC ACAGGAGAGT GGGATCGAGA TCAGATAAGA
    ATTAAGTTCT GGAAAAAGTG TAACGACAGC TTCGTGAAAA ATTCCATCTC CAGCAAAGCT
    GCATCGGATT TCTTTTTGAA TCAGGGCAAT GTGGTGTCTG AATCCCCAGT TCGTGAGAAG
    ATAAACAGCA TTTCGGAGAG ACATCTGCTG CTTACCCTGG CTGGCCATTG GCTCGCCAAG
    GATGTCTCGG TGGCATTGGA CAAATTGGAG GAAATAGAGA AACAGATCTG GATTTGTCGC
    ATCACACAAC AAATGTTATC CACAGATGAG GGGCCAGGCA AGTCGGGACG CTCCTCCTGT
    GCTGCAGCGA ATGGGGATTT TACTTTTGAT AACTTGGCCA AGGAGTTTTC TTTTTCAAAG
    CTGCCTGCTC TGAACACACC AAACTACTTA AACGTGGAGG GTCTTGAATT GACGGGGTGT
    CAGCAGACCT TGGCTGCGGA GGAGGAGGAG TCGCTCAGGT TTCTGGTCGG ATGCCTTCTC
    GATGAAGGAA GCGTGCACGA GGCCAGCCGC GTTTGTCGCT ATTTCCATTT CTACAGCAGA
    GACGTTTCAC TGGTGTTGCA CTGCAGAGCC CTGGCCGCAG GGGAGGCTGA CCTGGACAGA
    CTGCACGCAG ATATTCAGGC TCTTGTAGCA ATAGAGAAGA CACAGGACAG CAGTGCATCG
    CAGCAGAGCA GAGTCCCGAG CACATCGAGC TTAGAGGACT GGACACACAT TGCAGATCCA
    TCGCCGGATG ATCAGATTGT CGTCAGTCTG AAGGCCCTCA CAGATGAATG TGTCCATGGG
    AGAAACTACT GCAGACAGGT TCTGTGTCTG TATGAACTTT CTAAGGAACT GAACTGCTCC
    TACAGTGAGA TGTCAGCCCT TGACCCTGAG AAAGTGCTTC GAGCAATTCT GTCATCCCAG
    CAGGCAGAGC GCTGCAGGAG GGCTCAGGCG TTCATCACCA CGCAGGGCCT GCAGCCCGAA
    ACTGTGGCAG AGCTGGTGGC CAAAGAGGTC GTGCAGGAGT TACTGGCCTC TTCAGAAGGA
    AAAGGACAGA AGCAGGTTTC CAACCCTGCA GCTGAGAGCC AGGCGTTCCT GCAGCTCGCA
    AAGCTGTGCC AGGACCACAC GCTGGTGGGC ATGAAGCTGC TGGAGAAAAT TTCCTCTGTG
    CCACGTGGAG AACTGTCATG CATTACAGAA CTGCTGATTC TGGCCCATAA TTGCTTTAGT
    CTGACTTGCC ACATGGAAGG AATCACCAGA GTCCTGCAGG CAGCACGGCT GCTCACAGAT
    GAGCATTTGG CACCTAATGA TGAGTATGGA CTTATGGTTC GTCTTCTCAC TGGGATCGGC
    AGATACAATG AGATGACCTA CATCTTTGAG CTGCTGCACG AGAAGCATTA CTTTGAAGTG
    CTCATGAGGA AGAAGTTGGA CCCGAGTGGG ACACTGAAGA CAGCTCTGCT GGATTACATC
    AAGCGCTGCC GCCCAGGGGA CAGTGAGAAG CACAACATGA TTGCCCTTTG CTTCAGCATG
    TGCCGGGAAA TTGGAGAGAA TCACGAGGCT GCTGCTTGCA TACAGCTGAA ACTCATTGAA
    TCTCAGCCAT GGGAGGAGTC CCTTCAGGAT GTTGCAAACT TAAAGAAACT GCTGATGAAA
    GCTCTGACCC TCTTCATTGA TGCAGCAGAA AGTTACTCTA AGGACTCCTG TGTCCGCCAG
    GCGCTGCGCT GCCGCCGCCT CACAAAGCTG ATCACTCTGC AGCTGCATTT TCTGAGCACC
    GGTCAGAGCA CGATGCTGAT TAACAGGAAC AGGCAGAACC TCATGGAATG CATCGTGTCC
    CTGCCTCGAT TCTATCAGGC GGCGGTTGTG GCCGAGGCAT ATGAGTTTGT TCCAGATTGG
    GCTGAAGTTC TGTACCAACA AGTGATCGCT AAGGGAGACT TCAGCTACTT AGAAGAATTT
    AAACAGCAGA GACTGCTGAA GCCTGCCATG TTTGAGGAGA TTGCGAAGAA GTGTAAGCAG
    CACCCACCCA GCGATGCTGC TCTGAGGAAT CTGAAGAAGC TGCTCGCTTA CTGCGAGGAC
    ATCTCCACGT ACTACAGGCT GGCATATGAT AACAAGTTCT ATGATGTGGT CAATATGCTC
    CTGAAGGACG CGCAGACTGG ATGCTGCCTT AATGATATGT TATCCAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009735.1
    CDS244..6594
    Translation

    Target ORF information:

    RefSeq Version XM_025153967.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153967.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGAGCTGC TGTCCTCGCC TTTCCGCACA GACAGGTCAT GGAAGGCACA CTTATCCTCC 
    CTGTGCAGCA CAATAAGGAA AAGAAGATCA CGTTCCCCAC ATTTGCTATG TGATATGAAC
    TTACCTTGGT ATCAGTATTT TACCCACCTT GAAGATCACG ATCCCTTAAT CTATGAAACC
    TCTGAGAAGG CACTGGCCAT TGCTCCAAAG GCTTTTCCAC GTGTCCTTTC CAACCCATCG
    CAGAGTGCTG GCACAAAGCT CAGTGATATG GGGCAGCAGT GGAAACAAAT CCTCCCCGTA
    CAGTTATCAC AAGAAGTGGT GAAACTGGAG TGTAAATCTG TGACTGGAGC CAAAGCACTG
    TTTGTGATCC TAACAGATGA CAAGGCTTTG ACACTTGCTC TCTGGGATTT CGAGTCCCAG
    GATGTAGCAC ATTACCAGTT TGGCAAAAAC AGCGTTTATG TGGATTGTAA TGAGGAGGTG
    CCATTATGCC TGCTGCTGAC AGAGTGTGGC CTCTCCTTAA TTCTGTTTGG TTTAACTCAA
    GAGGAGTTTT TGAATAGACT GATGATCTAT GGCAGCGCTG GAGTCGTAGA CTCTGTATGT
    CACCTCAATG GATGGGGACG GTGTTCAATT CCAGTACATG CATTGGAGGC TGGCTTGGAA
    AATCGTCAGC TAGACACAGT GGACTTATTT TTAAAAAGCA AAGAAAGTCT TTTCAATCTC
    TCTGCGCTGC GCTCCGAAGC CCAGCAGTCT GCAGATGGTG CATCCTGCTC TTTTCTGAAA
    AATTTGCAAG AGCTGAGACC AGCTTTAAAC TTGCTTTGCT CAGCAATCAG AGAGAACGAC
    ATGGAGCCGC AGAGCAAACA CTTCTCAGAG CAGCTGCTGA ACCTCACGCT GACTTTCCTT
    AGCAAGCAGC TTGAAGAAAT TTTTGTCCAC ACAGAGGAAC CTGATAGATA CCTGCAGAAG
    GCTGCAGAGA TTTTAACTGA TTACATCATT GAACTTAGGA CGTTTATGAA AAACTATCCT
    CGATCACAAG TAAATACCAC AAATGTAGGG AGTGATTCTG ATGAAGACCT GCCTGCAATA
    GAGGAAAGCC AGATGTGGGA AAAACTGACA CCTGAGGAAG TCATTGCTGA AGCCATCTTA
    AGCAACAAAA TGCCAGAGGC CCAGATCTTC TTCCGAATGC ACCGGCATCC TGCTCAGAGC
    CTTCAGGAAC TCATTCGGAC AGGTTTGGAT CTGGTCTATG GCCATCTTCT GAAGGGCAAT
    ACCAGAGAGG CCTCAGCACT TCTGAAGAAC ATGGGTTTTG ATGTGAAGCA GCAGTTGCAT
    AAGATATGTT TCTACACGGC TGATAAACAC GTACGAGATT TGCTGGTCAA AGTTTTACAA
    GAAGAAAATT ATTTTTCTGA AAAAGAGAAG AAAATGATAG ACTTTGTGCA TCAGGTTGAA
    AGCTTGTACT CGGAGTCCTT TCAAGAAAAT AAAGAGATGC AATCTCTTTC CAGTGTAAGG
    AACTGGAGAC AGGAACAAGA CTCTTCCAAA CATTTGGCCA CTCTGGAGTC ATTGATGGAT
    CATGATGGAC GCCGGGTGCC CAGCAGGGAG GTCAGGGTGA TGCTGAACTG GGCTCAGTGC
    TGGGATGAGC TCCTTCAGGA TATGATTCTT CTCCCCAGAA AACAAAGCCA AGGACTTGCC
    ACATGTAACC CTGAGGCTCT TTGGATGTAC CTGACAGCCC AGCATGATTG GCTTAATATC
    AGTTCATGGA TTGAAGAATT TGAGCCCAAC CACAGCTTAT GTGGACAAAG TAAATGGCCA
    CCCCTGGCTG CAAGCATTGT TGACCAACAC ACGTTGTGTA GTGGCTACAT GAGAAATGAA
    ATATTGAACA AGCTGGCCAG AAATGGGATT TTTGTCCTTT CTGAATTGGA AGACTTTGAA
    CTTCTGCTCC AAAGATTGGT TTCCCTTGGA GGAGTACTGC AAAATCCTCA CCCTGTTCCA
    AAGTACAGTG CTGCGAGTGG TTTGGACTTC CATGCTCGTT TTGTCCTTCA CTGCTTAGAA
    CACAATTTGC AGTATATGTT ATACACATAC CTAGAATACT ACAGTTTAAC TCCTTCCAAC
    TGCCCTATTT TGGATGACAA GGAACTGCAT GAAGCACATC CCTGGTTTGA ATTCCTCGTG
    CAGTGCCGGG GGGTTGCCAG TAACCCACGA GATCCAAAGA TGATTTTTCA GGCGAGTCTA
    GCAAATGCTC AGATCTTGAT TCCTAGTAAT CAAGCCAGCG TGAGTAGCAT GCTGCTGGAG
    GGACGTACAC TGTTAGCACT TGCTACCACC ATGTATGCAC CTGGAGGCAT TGATCAGGTT
    CTTCAGAATG AAGATACAGA GAAACCACTA AAGAAAGTTG ATCCTCAGCT CTTAAAAATG
    GCATTGAGTC CTTACCCCAA ACTCAGAGCT GCTCTTTTTC CCCCGTGCAC CTCCCATGGA
    ATTTTGCCAC CTGACATTTC CCTTTATCAT CTTGTTCAGT CATTAGTGCC GTTTGATCCC
    AGTAAGTTAT TTGGCTGGCA GTCAACAAAT ACCCTTGCTG TATCAGATGC ATCAAGTGAC
    CTTCCTCATT TCTCATCCCC TGAACTGGTG AACAAATATG CTGTAATGGA ACGACTGGAC
    TTTTTGTACT ACCTGCGCCA TGGACGCCCA TCGTTTGCCT TTGGTACATT TTTGGTCCAG
    CAGTTGGTCA AGAGCAAAAG CCCCAAACAG TTGATTCAGC AAGCAGGAAA TGAAGCCTAT
    CTTCTAGGCC TTTCTTTCTT CAATACGCCT TCAGTAGCAG CTGCCTGTGT GTGCTTTCTG
    GAGCTGCTTG GGCTCAGCAG CCTCAAACTG AGAGTTGATA TTAAGGTTGC AAACATGATA
    TTCATTTACA AAACCAGAAA TGAAGAAACT CAGCACAATC AAATAAGGGA ATCTCTGGTT
    GAACAGCTAA TGAGGTTGGC TGATGGTGAA AAAGCAGCAA CAGAAGAAGT TCTTAAATCC
    TTAGAAGAAG CCTTTTGGGA TTCAATTGAG CATCAAGGAA TAAAGAGGAC ATCCAGTGAC
    TCCAGACAGC AGTGGTCTTT GGTGATGCAG TTCTGCAAAC TCCATGCCAT GAAACTGAGC
    ACCTCATACC TAAAGGAGTG TGCTAAATCC AATGACTGGC TGCAGTTCAT CATCCAAGCC
    CAGCTGTACA GCTACAAGCC AGATGAGGTC TCATCTGTCC TTGAAGGCTT CGCTCCCATT
    ATACAGCGTC ACCTGATGTT GGCACTGGAG AAGTCACCGT TTCTGCACGC TGCTGCGGGG
    GCTCCTGAGG GCAGCACTGG GAGCAGATGT GCAGACAGTC TCTTCCAAAT CCTTTTCCGG
    TGTCAGGAGA GCCCCAATGC TTCGTGTCAC CTTTTGGCTG AAGGACTGAG AGAACATGCT
    CCCATTCTGA GTGTTTTAGC AGCGTGTTCC CCTGATGCTG ACATTATTCA CTGTCTCTGT
    GTCTGGATAA TTACCAGTGT GGATGATGTT ACCAGATCTG AAGCGACAAA ACATATTCAG
    AGCTCAGTAG AAAATCACAA GTGGGATCTT CATGACCTGT CGCTTATCTG GAAAGTGCTT
    TTAAGAAAGC AAAAATGTCA AACACTTATA AGGGGATTCC AGCTCTTCTT AAAGGATTCC
    CCTTTACTGC ATATACTGGA GATGTACGAT CTGTGCATGA ACTGTAAAAG GTACTATGAA
    GCCAAAACCA AACTGGACGA GTTTCAGAAA CACCTCACAA ATCTGAAGAC CACCGATGGA
    GCGGCAGCTT CAGGAGTCCC TGTGCAATGG TTGGAGTCAC AGGCCTTGTT TCTTCTTGAA
    CTGATGATGC AGCAGTGTAG GACAGAATAT GAATTGGGAA AGCTCTTACA GCTGTTTGCT
    GCAACAGACA ACCTTCTCCT TGATGGCCCT TATGTGAAGA AACTGTGTGC GCTCAGTGAG
    ACCCTGAGGG ATTCCTCCGT GCCCATTAAT CGCTCCATGC TGACCAGGTA CACCCCAGAA
    ACGTTTCAGA AGGAGTGCAC ATCGATTCTG GAGCAGCTCC AGGAGAAAGG AATGTTCTCT
    CTGGCTCGGG AGGTGGCAGA GCTGGCTGAA CTGCCTGCGG ACAGCGTGGT TACCCAAGAG
    ATTCTGAGGG ATATACGTCT GTTAAGAGAC ACAGGAGAGT GGGATCGAGA TCAGATAAGA
    ATTAAGTTCT GGAAAAAGTG TAACGACAGC TTCGTGAAAA ATTCCATCTC CAGCAAAGCT
    GCATCGGATT TCTTTTTGAA TCAGGGCAAT GTGGTGTCTG AATCCCCAGT TCGTGAGAAG
    ATAAACAGCA TTTCGGAGAG ACATCTGCTG CTTACCCTGG CTGGCCATTG GCTCGCCAAG
    GATGTCTCGG TGGCATTGGA CAAATTGGAG GAAATAGAGA AACAGATCTG GATTTGTCGC
    ATCACACAAC AAATGTTATC CACAGATGAG GGGCCAGGCA AGTCGGGACG CTCCTCCTGT
    GCTGCAGCGA ATGGGGATTT TACTTTTGAT AACTTGGCCA AGGAGTTTTC TTTTTCAAAG
    CTGCCTGCTC TGAACACACC AAACTACTTA AACGTGGAGG GTCTTGAATT GACGGGGTGT
    CAGCAGACCT TGGCTGCGGA GGAGGAGGAG TCGCTCAGGT TTCTGGTCGG ATGCCTTCTC
    GATGAAGGAA GCGTGCACGA GGCCAGCCGC GTTTGTCGCT ATTTCCATTT CTACAGCAGA
    GACGTTTCAC TGGTGTTGCA CTGCAGAGCC CTGGCCGCAG GGGAGGCTGA CCTGGACAGA
    CTGCACGCAG ATATTCAGGC TCTTGTAGCA ATAGAGAAGA CACAGGACAG CAGTGCATCG
    CAGCAGAGCA GAGTCCCGAG CACATCGAGC TTAGAGGACT GGACACACAT TGCAGATCCA
    TCGCCGGATG ATCAGATTGT CGTCAGTCTG AAGGCCCTCA CAGATGAATG TGTCCATGGG
    AGAAACTACT GCAGACAGGT TCTGTGTCTG TATGAACTTT CTAAGGAACT GAACTGCTCC
    TACAGTGAGA TGTCAGCCCT TGACCCTGAG AAAGTGCTTC GAGCAATTCT GTCATCCCAG
    CAGGCAGAGC GCTGCAGGAG GGCTCAGGCG TTCATCACCA CGCAGGGCCT GCAGCCCGAA
    ACTGTGGCAG AGCTGGTGGC CAAAGAGGTC GTGCAGGAGT TACTGGCCTC TTCAGAAGGA
    AAAGGACAGA AGCAGGTTTC CAACCCTGCA GCTGAGAGCC AGGCGTTCCT GCAGCTCGCA
    AAGCTGTGCC AGGACCACAC GCTGGTGGGC ATGAAGCTGC TGGAGAAAAT TTCCTCTGTG
    CCACGTGGAG AACTGTCATG CATTACAGAA CTGCTGATTC TGGCCCATAA TTGCTTTAGT
    CTGACTTGCC ACATGGAAGG AATCACCAGA GTCCTGCAGG CAGCACGGCT GCTCACAGAT
    GAGCATTTGG CACCTAATGA TGAGTATGGA CTTATGGTTC GTCTTCTCAC TGGGATCGGC
    AGATACAATG AGATGACCTA CATCTTTGAG CTGCTGCACG AGAAGCATTA CTTTGAAGTG
    CTCATGAGGA AGAAGTTGGA CCCGAGTGGG ACACTGAAGA CAGCTCTGCT GGATTACATC
    AAGCGCTGCC GCCCAGGGGA CAGTGAGAAG CACAACATGA TTGCCCTTTG CTTCAGCATG
    TGCCGGGAAA TTGGAGAGAA TCACGAGGCT GCTGCTTGCA TACAGCTGAA ACTCATTGAA
    TCTCAGCCAT GGGAGGAGTC CCTTCAGGAT GTTGCAAACT TAAAGAAACT GCTGATGAAA
    GCTCTGACCC TCTTCATTGA TGCAGCAGAA AGTTACTCTA AGGACTCCTG TGTCCGCCAG
    GCGCTGCGCT GCCGCCGCCT CACAAAGCTG ATCACTCTGC AGCTGCATTT TCTGAGCACC
    GGTCAGAGCA CGATGCTGAT TAACAGGAAC AGGCAGAACC TCATGGAATG CATCGTGTCC
    CTGCCTCGAT TCTATCAGGC GGCGGTTGTG GCCGAGGCAT ATGAGTTTGT TCCAGATTGG
    GCTGAAGTTC TGTACCAACA AGTGATCGCT AAGGGAGACT TCAGCTACTT AGAAGAATTT
    AAACAGCAGA GACTGCTGAA GCCTGCCATG TTTGAGGAGA TTGCGAAGAA GTGTAAGCAG
    CACCCACCCA GCGATGCTGC TCTGAGGAAT CTGAAGAAGC TGCTCGCTTA CTGCGAGGAC
    ATCTCCACGT ACTACAGGCT GGCATATGAT AACAAGTTCT ATGATGTGGT CAATATGCTC
    CTGAAGGACG CGCAGACTGG ATGCTGCCTT AATGATATGT TATCCAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa13353
    Clone ID Related Accession (Same CDS sequence) XM_003641831.3 , XM_003641831.4
    Accession Version XM_003641831.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7278bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product spatacsin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455925.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_003641831.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
    GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC
    CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG
    CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT
    GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC
    TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG
    GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG
    AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG
    CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT
    TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT
    TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA
    CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC
    ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT
    TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT
    CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG
    GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC
    AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT
    TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA
    GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT
    TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG
    CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT
    AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA
    CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC
    GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC
    TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC
    AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA
    GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA
    AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA
    GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG
    CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG
    CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA
    GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA
    CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT
    GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT
    GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC
    CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG
    GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG
    GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA
    CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA
    ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA
    GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT
    TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC
    AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC
    CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG
    ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC
    AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG
    TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT
    GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA
    GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT
    GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA
    GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA
    GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT
    CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA
    GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG
    TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG
    AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT
    CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT
    GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC
    CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC
    AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG
    TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG
    ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA
    GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA
    GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG
    CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA
    GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT
    CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC
    TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT
    GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA
    TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG
    TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA
    GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT
    TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT
    GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC
    AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT
    GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG
    GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG
    TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC
    CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG
    GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA
    TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT
    GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC
    TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG
    CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG
    CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA
    GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC
    GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG
    GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT
    ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA
    ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG
    CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC
    TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC
    GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG
    CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT
    TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG
    GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA
    GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA
    GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG
    GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGTTCT GTGTCTGTAT
    GAACTTTCTA AGGAACTGAA CTGCTCCTAC AGTGAGATGT CAGCCCTTGA CCCTGAGAAA
    GTGCTTCGAG CAATTCTGTC ATCCCAGCAG GCAGAGCGCT GCAGGAGGGC TCAGGCGTTC
    ATCACCACGC AGGGCCTGCA GCCCGAAACT GTGGCAGAGC TGGTGGCCAA AGAGGTCGTG
    CAGGAGTTAC TGGCCTCTTC AGAAGGAAAA GGACAGAAGC AGGTTTCCAA CCCTGCAGCT
    GAGAGCCAGG CGTTCCTGCA GCTCGCAAAG CTGTGCCAGG ACCACACGCT GGTGGGCATG
    AAGCTGCTGG AGAAAATTTC CTCTGTGCCA CGTGGAGAAC TGTCATGCAT TACAGAACTG
    CTGATTCTGG CCCATAATTG CTTTAGTCTG ACTTGCCACA TGGAAGGAAT CACCAGAGTC
    CTGCAGGCAG CACGGCTGCT CACAGATGAG CATTTGGCAC CTAATGATGA GTATGGACTT
    ATGGTTCGTC TTCTCACTGG GATCGGCAGA TACAATGAGA TGACCTACAT CTTTGAGCTG
    CTGCACGAGA AGCATTACTT TGAAGTGCTC ATGAGGAAGA AGTTGGACCC GAGTGGGACA
    CTGAAGACAG CTCTGCTGGA TTACATCAAG CGCTGCCGCC CAGGGGACAG TGAGAAGCAC
    AACATGATTG CCCTTTGCTT CAGCATGTGC CGGGAAATTG GAGAGAATCA CGAGGCTGCT
    GCTTGCATAC AGCTGAAACT CATTGAATCT CAGCCATGGG AGGAGTCCCT TCAGGATGTT
    GCAAACTTAA AGAAACTGCT GATGAAAGCT CTGACCCTCT TCATTGATGC AGCAGAAAGT
    TACTCTAAGG ACTCCTGTGT CCGCCAGGCG CTGCGCTGCC GCCGCCTCAC AAAGCTGATC
    ACTCTGCAGC TGCATTTTCT GAGCACCGGT CAGAGCACGA TGCTGATTAA CAGGAACAGG
    CAGAACCTCA TGGAATGCAT CGTGTCCCTG CCTCGATTCT ATCAGGCGGC GGTTGTGGCC
    GAGGCATATG AGTTTGTTCC AGATTGGGCT GAAGTTCTGT ACCAACAAGT GATCGCTAAG
    GGAGACTTCA GCTACTTAGA AGAATTTAAA CAGCAGAGAC TGCTGAAGCC TGCCATGTTT
    GAGGAGATTG CGAAGAAGTG TAAGCAGCAC CCACCCAGCG ATGCTGCTCT GAGGAATCTG
    AAGAAGCTGC TCGCTTACTG CGAGGACATC TCCACGTACT ACAGGCTGGC ATATGATAAC
    AAGTTCTATG ATGTGGTCAA TATGCTCCTG AAGGACGCGC AGACTGGATG CTGCCTTAAT
    GATATGTTAT CCAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003641879.2
    CDS104..7381
    Translation

    Target ORF information:

    RefSeq Version XM_003641831.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003641831.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
    GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC
    CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG
    CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT
    GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC
    TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG
    GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG
    AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG
    CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT
    TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT
    TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA
    CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC
    ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT
    TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT
    CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG
    GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC
    AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT
    TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA
    GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT
    TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG
    CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT
    AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA
    CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC
    GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC
    TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC
    AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA
    GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA
    AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA
    GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG
    CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG
    CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA
    GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA
    CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT
    GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT
    GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC
    CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG
    GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG
    GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA
    CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA
    ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA
    GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT
    TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC
    AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC
    CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG
    ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC
    AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG
    TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT
    GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA
    GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT
    GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA
    GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA
    GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT
    CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA
    GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG
    TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG
    AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT
    CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT
    GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC
    CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC
    AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG
    TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG
    ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA
    GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA
    GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG
    CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA
    GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT
    CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC
    TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT
    GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA
    TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG
    TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA
    GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT
    TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT
    GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC
    AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT
    GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG
    GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG
    TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC
    CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG
    GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA
    TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT
    GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC
    TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG
    CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG
    CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA
    GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC
    GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG
    GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT
    ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA
    ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG
    CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC
    TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC
    GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG
    CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT
    TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG
    GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA
    GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA
    GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG
    GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGTTCT GTGTCTGTAT
    GAACTTTCTA AGGAACTGAA CTGCTCCTAC AGTGAGATGT CAGCCCTTGA CCCTGAGAAA
    GTGCTTCGAG CAATTCTGTC ATCCCAGCAG GCAGAGCGCT GCAGGAGGGC TCAGGCGTTC
    ATCACCACGC AGGGCCTGCA GCCCGAAACT GTGGCAGAGC TGGTGGCCAA AGAGGTCGTG
    CAGGAGTTAC TGGCCTCTTC AGAAGGAAAA GGACAGAAGC AGGTTTCCAA CCCTGCAGCT
    GAGAGCCAGG CGTTCCTGCA GCTCGCAAAG CTGTGCCAGG ACCACACGCT GGTGGGCATG
    AAGCTGCTGG AGAAAATTTC CTCTGTGCCA CGTGGAGAAC TGTCATGCAT TACAGAACTG
    CTGATTCTGG CCCATAATTG CTTTAGTCTG ACTTGCCACA TGGAAGGAAT CACCAGAGTC
    CTGCAGGCAG CACGGCTGCT CACAGATGAG CATTTGGCAC CTAATGATGA GTATGGACTT
    ATGGTTCGTC TTCTCACTGG GATCGGCAGA TACAATGAGA TGACCTACAT CTTTGAGCTG
    CTGCACGAGA AGCATTACTT TGAAGTGCTC ATGAGGAAGA AGTTGGACCC GAGTGGGACA
    CTGAAGACAG CTCTGCTGGA TTACATCAAG CGCTGCCGCC CAGGGGACAG TGAGAAGCAC
    AACATGATTG CCCTTTGCTT CAGCATGTGC CGGGAAATTG GAGAGAATCA CGAGGCTGCT
    GCTTGCATAC AGCTGAAACT CATTGAATCT CAGCCATGGG AGGAGTCCCT TCAGGATGTT
    GCAAACTTAA AGAAACTGCT GATGAAAGCT CTGACCCTCT TCATTGATGC AGCAGAAAGT
    TACTCTAAGG ACTCCTGTGT CCGCCAGGCG CTGCGCTGCC GCCGCCTCAC AAAGCTGATC
    ACTCTGCAGC TGCATTTTCT GAGCACCGGT CAGAGCACGA TGCTGATTAA CAGGAACAGG
    CAGAACCTCA TGGAATGCAT CGTGTCCCTG CCTCGATTCT ATCAGGCGGC GGTTGTGGCC
    GAGGCATATG AGTTTGTTCC AGATTGGGCT GAAGTTCTGT ACCAACAAGT GATCGCTAAG
    GGAGACTTCA GCTACTTAGA AGAATTTAAA CAGCAGAGAC TGCTGAAGCC TGCCATGTTT
    GAGGAGATTG CGAAGAAGTG TAAGCAGCAC CCACCCAGCG ATGCTGCTCT GAGGAATCTG
    AAGAAGCTGC TCGCTTACTG CGAGGACATC TCCACGTACT ACAGGCTGGC ATATGATAAC
    AAGTTCTATG ATGTGGTCAA TATGCTCCTG AAGGACGCGC AGACTGGATG CTGCCTTAAT
    GATATGTTAT CCAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa13353
    Clone ID Related Accession (Same CDS sequence) XM_003641831.3 , XM_003641831.4
    Accession Version XM_003641831.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7278bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product spatacsin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_003641831.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
    GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC
    CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG
    CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT
    GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC
    TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG
    GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG
    AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG
    CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT
    TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT
    TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA
    CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC
    ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT
    TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT
    CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG
    GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC
    AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT
    TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA
    GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT
    TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG
    CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT
    AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA
    CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC
    GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC
    TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC
    AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA
    GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA
    AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA
    GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG
    CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG
    CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA
    GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA
    CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT
    GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT
    GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC
    CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG
    GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG
    GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA
    CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA
    ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA
    GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT
    TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC
    AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC
    CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG
    ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC
    AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG
    TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT
    GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA
    GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT
    GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA
    GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA
    GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT
    CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA
    GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG
    TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG
    AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT
    CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT
    GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC
    CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC
    AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG
    TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG
    ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA
    GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA
    GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG
    CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA
    GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT
    CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC
    TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT
    GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA
    TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG
    TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA
    GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT
    TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT
    GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC
    AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT
    GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG
    GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG
    TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC
    CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG
    GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA
    TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT
    GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC
    TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG
    CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG
    CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA
    GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC
    GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG
    GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT
    ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA
    ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG
    CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC
    TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC
    GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG
    CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT
    TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG
    GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA
    GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA
    GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG
    GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGTTCT GTGTCTGTAT
    GAACTTTCTA AGGAACTGAA CTGCTCCTAC AGTGAGATGT CAGCCCTTGA CCCTGAGAAA
    GTGCTTCGAG CAATTCTGTC ATCCCAGCAG GCAGAGCGCT GCAGGAGGGC TCAGGCGTTC
    ATCACCACGC AGGGCCTGCA GCCCGAAACT GTGGCAGAGC TGGTGGCCAA AGAGGTCGTG
    CAGGAGTTAC TGGCCTCTTC AGAAGGAAAA GGACAGAAGC AGGTTTCCAA CCCTGCAGCT
    GAGAGCCAGG CGTTCCTGCA GCTCGCAAAG CTGTGCCAGG ACCACACGCT GGTGGGCATG
    AAGCTGCTGG AGAAAATTTC CTCTGTGCCA CGTGGAGAAC TGTCATGCAT TACAGAACTG
    CTGATTCTGG CCCATAATTG CTTTAGTCTG ACTTGCCACA TGGAAGGAAT CACCAGAGTC
    CTGCAGGCAG CACGGCTGCT CACAGATGAG CATTTGGCAC CTAATGATGA GTATGGACTT
    ATGGTTCGTC TTCTCACTGG GATCGGCAGA TACAATGAGA TGACCTACAT CTTTGAGCTG
    CTGCACGAGA AGCATTACTT TGAAGTGCTC ATGAGGAAGA AGTTGGACCC GAGTGGGACA
    CTGAAGACAG CTCTGCTGGA TTACATCAAG CGCTGCCGCC CAGGGGACAG TGAGAAGCAC
    AACATGATTG CCCTTTGCTT CAGCATGTGC CGGGAAATTG GAGAGAATCA CGAGGCTGCT
    GCTTGCATAC AGCTGAAACT CATTGAATCT CAGCCATGGG AGGAGTCCCT TCAGGATGTT
    GCAAACTTAA AGAAACTGCT GATGAAAGCT CTGACCCTCT TCATTGATGC AGCAGAAAGT
    TACTCTAAGG ACTCCTGTGT CCGCCAGGCG CTGCGCTGCC GCCGCCTCAC AAAGCTGATC
    ACTCTGCAGC TGCATTTTCT GAGCACCGGT CAGAGCACGA TGCTGATTAA CAGGAACAGG
    CAGAACCTCA TGGAATGCAT CGTGTCCCTG CCTCGATTCT ATCAGGCGGC GGTTGTGGCC
    GAGGCATATG AGTTTGTTCC AGATTGGGCT GAAGTTCTGT ACCAACAAGT GATCGCTAAG
    GGAGACTTCA GCTACTTAGA AGAATTTAAA CAGCAGAGAC TGCTGAAGCC TGCCATGTTT
    GAGGAGATTG CGAAGAAGTG TAAGCAGCAC CCACCCAGCG ATGCTGCTCT GAGGAATCTG
    AAGAAGCTGC TCGCTTACTG CGAGGACATC TCCACGTACT ACAGGCTGGC ATATGATAAC
    AAGTTCTATG ATGTGGTCAA TATGCTCCTG AAGGACGCGC AGACTGGATG CTGCCTTAAT
    GATATGTTAT CCAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003641879.2
    CDS143..7420
    Translation

    Target ORF information:

    RefSeq Version XM_003641831.4
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003641831.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
    GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC
    CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG
    CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT
    GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC
    TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG
    GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG
    AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG
    CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT
    TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT
    TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA
    CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC
    ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT
    TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT
    CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG
    GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC
    AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT
    TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA
    GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT
    TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG
    CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT
    AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA
    CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC
    GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC
    TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC
    AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA
    GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA
    AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA
    GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG
    CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG
    CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA
    GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA
    CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT
    GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT
    GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC
    CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG
    GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG
    GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA
    CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA
    ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA
    GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT
    TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC
    AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC
    CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG
    ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC
    AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG
    TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT
    GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA
    GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT
    GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA
    GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA
    GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT
    CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA
    GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG
    TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG
    AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT
    CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT
    GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC
    CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC
    AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG
    TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG
    ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA
    GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA
    GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG
    CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA
    GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT
    CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC
    TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT
    GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA
    TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG
    TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA
    GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT
    TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT
    GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC
    AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT
    GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG
    GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG
    TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC
    CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG
    GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA
    TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT
    GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC
    TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG
    CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG
    CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA
    GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC
    GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG
    GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT
    ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA
    ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG
    CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC
    TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC
    GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG
    CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT
    TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG
    GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA
    GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA
    GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG
    GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGTTCT GTGTCTGTAT
    GAACTTTCTA AGGAACTGAA CTGCTCCTAC AGTGAGATGT CAGCCCTTGA CCCTGAGAAA
    GTGCTTCGAG CAATTCTGTC ATCCCAGCAG GCAGAGCGCT GCAGGAGGGC TCAGGCGTTC
    ATCACCACGC AGGGCCTGCA GCCCGAAACT GTGGCAGAGC TGGTGGCCAA AGAGGTCGTG
    CAGGAGTTAC TGGCCTCTTC AGAAGGAAAA GGACAGAAGC AGGTTTCCAA CCCTGCAGCT
    GAGAGCCAGG CGTTCCTGCA GCTCGCAAAG CTGTGCCAGG ACCACACGCT GGTGGGCATG
    AAGCTGCTGG AGAAAATTTC CTCTGTGCCA CGTGGAGAAC TGTCATGCAT TACAGAACTG
    CTGATTCTGG CCCATAATTG CTTTAGTCTG ACTTGCCACA TGGAAGGAAT CACCAGAGTC
    CTGCAGGCAG CACGGCTGCT CACAGATGAG CATTTGGCAC CTAATGATGA GTATGGACTT
    ATGGTTCGTC TTCTCACTGG GATCGGCAGA TACAATGAGA TGACCTACAT CTTTGAGCTG
    CTGCACGAGA AGCATTACTT TGAAGTGCTC ATGAGGAAGA AGTTGGACCC GAGTGGGACA
    CTGAAGACAG CTCTGCTGGA TTACATCAAG CGCTGCCGCC CAGGGGACAG TGAGAAGCAC
    AACATGATTG CCCTTTGCTT CAGCATGTGC CGGGAAATTG GAGAGAATCA CGAGGCTGCT
    GCTTGCATAC AGCTGAAACT CATTGAATCT CAGCCATGGG AGGAGTCCCT TCAGGATGTT
    GCAAACTTAA AGAAACTGCT GATGAAAGCT CTGACCCTCT TCATTGATGC AGCAGAAAGT
    TACTCTAAGG ACTCCTGTGT CCGCCAGGCG CTGCGCTGCC GCCGCCTCAC AAAGCTGATC
    ACTCTGCAGC TGCATTTTCT GAGCACCGGT CAGAGCACGA TGCTGATTAA CAGGAACAGG
    CAGAACCTCA TGGAATGCAT CGTGTCCCTG CCTCGATTCT ATCAGGCGGC GGTTGTGGCC
    GAGGCATATG AGTTTGTTCC AGATTGGGCT GAAGTTCTGT ACCAACAAGT GATCGCTAAG
    GGAGACTTCA GCTACTTAGA AGAATTTAAA CAGCAGAGAC TGCTGAAGCC TGCCATGTTT
    GAGGAGATTG CGAAGAAGTG TAAGCAGCAC CCACCCAGCG ATGCTGCTCT GAGGAATCTG
    AAGAAGCTGC TCGCTTACTG CGAGGACATC TCCACGTACT ACAGGCTGGC ATATGATAAC
    AAGTTCTATG ATGTGGTCAA TATGCTCCTG AAGGACGCGC AGACTGGATG CTGCCTTAAT
    GATATGTTAT CCAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa13354
    Clone ID Related Accession (Same CDS sequence) XM_004943936.3 , XM_004943936.2
    Accession Version XM_004943936.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product spatacsin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004943936.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
    GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC
    CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG
    CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT
    GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC
    TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG
    GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG
    AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG
    CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT
    TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT
    TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA
    CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC
    ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT
    TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT
    CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG
    GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC
    AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT
    TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA
    GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT
    TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG
    CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT
    AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA
    CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC
    GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC
    TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC
    AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA
    GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA
    AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA
    GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG
    CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG
    CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA
    GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA
    CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT
    GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT
    GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC
    CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG
    GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG
    GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA
    CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA
    ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA
    GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT
    TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC
    AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC
    CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG
    ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC
    AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG
    TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT
    GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA
    GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT
    GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA
    GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA
    GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT
    CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA
    GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG
    TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG
    AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT
    CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT
    GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC
    CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC
    AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG
    TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG
    ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA
    GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA
    GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG
    CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA
    GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT
    CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC
    TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT
    GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA
    TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG
    TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA
    GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT
    TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT
    GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC
    AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT
    GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG
    GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG
    TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC
    CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG
    GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA
    TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT
    GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC
    TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG
    CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG
    CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA
    GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC
    GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG
    GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT
    ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA
    ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG
    CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC
    TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC
    GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG
    CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT
    TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG
    GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA
    GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA
    GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG
    GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGTTCT GTGTCTGTAT
    GAACTTTCTA AGGAACTGAA CTGCTCCTAC AGTGAGATGT CAGCCCTTGA CCCTGAGAAA
    GTGCTTCGAG CAATTCTGTC ATCCCAGCAG GCAGAGCGCT GCAGGAGGGC TCAGGCGTTC
    ATCACCACGC AGGGCCTGCA GCCCGAAACT GTGGCAGAGC TGGTGGCCAA AGAGGTCGTG
    CAGGAGTTAC TGGCCTCTTC AGAAGGAAAA GGACAGAAGC AGGTTTCCAA CCCTGCAGCT
    GAGAGCCAGG CGTTCCTGCA GCTCGCAAAG CTGTGCCAGG ACCACACGCT GGTGGGCATG
    AAGCTGCTGG AGAAAATTTC CTCTGTGCCA CGTGGAGAAC TGTCATGCAT TACAGAACTG
    CTGATTCTGG CCCATAATTG CTTTAGTCTG ACTTGCCACA TGGAAGGAAT CACCAGAGTC
    CTGCAGGCAG CACGGCTGCT CACAGATGAG CATTTGGCAC CTAATGATGA GTATGGACTT
    ATGGTTCGTC TTCTCACTGG GATCGGCAGA TACAATGAGA TGACCTACAT CTTTGAGCTG
    CTGCACGAGA AGCATTACTT TGAAGTGCTC ATGAGGAAGA AGTTGGACCC GAGTGGGACA
    CTGAAGACAG CTCTGCTGGA TTACATCAAG CGCTGCCGCC CAGGGGACAG TGAGAAGCAC
    AACATGATTG CCCTTTGCTT CAGCATGTGC CGGGAAATTG GAGAGAATCA CGAGGCTGCT
    GCTTGCATAC AGCTGAAACT CATTGAATCT CAGCCATGGG AGGAGTCCCT TCAGGATGTT
    GCAAACTTAA AGAAACTGCT GATGAAAGCT CTGACCCTCT TCATTGATGC AGCAGAAAGT
    TACTCTAAGG CGCTGCGCTG CCGCCGCCTC ACAAAGCTGA TCACTCTGCA GCTGCATTTT
    CTGAGCACCG GTCAGAGCAC GATGCTGATT AACAGGAACA GGCAGAACCT CATGGAATGC
    ATCGTGTCCC TGCCTCGATT CTATCAGGCG GCGGTTGTGG CCGAGGCATA TGAGTTTGTT
    CCAGATTGGG CTGAAGTTCT GTACCAACAA GTGATCGCTA AGGGAGACTT CAGCTACTTA
    GAAGAATTTA AACAGCAGAG ACTGCTGAAG CCTGCCATGT TTGAGGAGAT TGCGAAGAAG
    TGTAAGCAGC ACCCACCCAG CGATGCTGCT CTGAGGAATC TGAAGAAGCT GCTCGCTTAC
    TGCGAGGACA TCTCCACGTA CTACAGGCTG GCATATGATA ACAAGTTCTA TGATGTGGTC
    AATATGCTCC TGAAGGACGC GCAGACTGGA TGCTGCCTTA ATGATATGTT ATCCAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943993.1
    CDS143..7402
    Translation

    Target ORF information:

    RefSeq Version XM_004943936.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943936.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
    GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC
    CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG
    CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT
    GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC
    TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG
    GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG
    AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG
    CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT
    TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT
    TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA
    CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC
    ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT
    TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT
    CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG
    GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC
    AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT
    TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA
    GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT
    TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG
    CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT
    AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA
    CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC
    GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC
    TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC
    AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA
    GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA
    AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA
    GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG
    CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG
    CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA
    GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA
    CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT
    GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT
    GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC
    CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG
    GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG
    GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA
    CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA
    ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA
    GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT
    TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC
    AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC
    CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG
    ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC
    AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG
    TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT
    GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA
    GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT
    GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA
    GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA
    GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT
    CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA
    GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG
    TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG
    AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT
    CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT
    GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC
    CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC
    AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG
    TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG
    ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA
    GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA
    GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG
    CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA
    GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT
    CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC
    TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT
    GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA
    TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG
    TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA
    GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT
    TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT
    GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC
    AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT
    GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG
    GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG
    TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC
    CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG
    GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA
    TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT
    GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC
    TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG
    CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG
    CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA
    GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC
    GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG
    GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT
    ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA
    ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG
    CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC
    TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC
    GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG
    CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT
    TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG
    GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA
    GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA
    GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG
    GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGTTCT GTGTCTGTAT
    GAACTTTCTA AGGAACTGAA CTGCTCCTAC AGTGAGATGT CAGCCCTTGA CCCTGAGAAA
    GTGCTTCGAG CAATTCTGTC ATCCCAGCAG GCAGAGCGCT GCAGGAGGGC TCAGGCGTTC
    ATCACCACGC AGGGCCTGCA GCCCGAAACT GTGGCAGAGC TGGTGGCCAA AGAGGTCGTG
    CAGGAGTTAC TGGCCTCTTC AGAAGGAAAA GGACAGAAGC AGGTTTCCAA CCCTGCAGCT
    GAGAGCCAGG CGTTCCTGCA GCTCGCAAAG CTGTGCCAGG ACCACACGCT GGTGGGCATG
    AAGCTGCTGG AGAAAATTTC CTCTGTGCCA CGTGGAGAAC TGTCATGCAT TACAGAACTG
    CTGATTCTGG CCCATAATTG CTTTAGTCTG ACTTGCCACA TGGAAGGAAT CACCAGAGTC
    CTGCAGGCAG CACGGCTGCT CACAGATGAG CATTTGGCAC CTAATGATGA GTATGGACTT
    ATGGTTCGTC TTCTCACTGG GATCGGCAGA TACAATGAGA TGACCTACAT CTTTGAGCTG
    CTGCACGAGA AGCATTACTT TGAAGTGCTC ATGAGGAAGA AGTTGGACCC GAGTGGGACA
    CTGAAGACAG CTCTGCTGGA TTACATCAAG CGCTGCCGCC CAGGGGACAG TGAGAAGCAC
    AACATGATTG CCCTTTGCTT CAGCATGTGC CGGGAAATTG GAGAGAATCA CGAGGCTGCT
    GCTTGCATAC AGCTGAAACT CATTGAATCT CAGCCATGGG AGGAGTCCCT TCAGGATGTT
    GCAAACTTAA AGAAACTGCT GATGAAAGCT CTGACCCTCT TCATTGATGC AGCAGAAAGT
    TACTCTAAGG CGCTGCGCTG CCGCCGCCTC ACAAAGCTGA TCACTCTGCA GCTGCATTTT
    CTGAGCACCG GTCAGAGCAC GATGCTGATT AACAGGAACA GGCAGAACCT CATGGAATGC
    ATCGTGTCCC TGCCTCGATT CTATCAGGCG GCGGTTGTGG CCGAGGCATA TGAGTTTGTT
    CCAGATTGGG CTGAAGTTCT GTACCAACAA GTGATCGCTA AGGGAGACTT CAGCTACTTA
    GAAGAATTTA AACAGCAGAG ACTGCTGAAG CCTGCCATGT TTGAGGAGAT TGCGAAGAAG
    TGTAAGCAGC ACCCACCCAG CGATGCTGCT CTGAGGAATC TGAAGAAGCT GCTCGCTTAC
    TGCGAGGACA TCTCCACGTA CTACAGGCTG GCATATGATA ACAAGTTCTA TGATGTGGTC
    AATATGCTCC TGAAGGACGC GCAGACTGGA TGCTGCCTTA ATGATATGTT ATCCAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa13354
    Clone ID Related Accession (Same CDS sequence) XM_004943936.3 , XM_004943936.2
    Accession Version XM_004943936.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product spatacsin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455925.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004943936.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
    GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC
    CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG
    CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT
    GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC
    TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG
    GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG
    AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG
    CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT
    TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT
    TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA
    CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC
    ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT
    TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT
    CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG
    GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC
    AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT
    TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA
    GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT
    TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG
    CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT
    AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA
    CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC
    GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC
    TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC
    AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA
    GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA
    AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA
    GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG
    CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG
    CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA
    GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA
    CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT
    GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT
    GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC
    CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG
    GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG
    GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA
    CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA
    ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA
    GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT
    TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC
    AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC
    CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG
    ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC
    AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG
    TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT
    GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA
    GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT
    GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA
    GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA
    GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT
    CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA
    GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG
    TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG
    AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT
    CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT
    GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC
    CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC
    AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG
    TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG
    ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA
    GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA
    GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG
    CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA
    GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT
    CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC
    TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT
    GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA
    TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG
    TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA
    GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT
    TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT
    GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC
    AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT
    GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG
    GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG
    TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC
    CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG
    GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA
    TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT
    GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC
    TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG
    CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG
    CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA
    GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC
    GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG
    GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT
    ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA
    ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG
    CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC
    TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC
    GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG
    CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT
    TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG
    GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA
    GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA
    GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG
    GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGTTCT GTGTCTGTAT
    GAACTTTCTA AGGAACTGAA CTGCTCCTAC AGTGAGATGT CAGCCCTTGA CCCTGAGAAA
    GTGCTTCGAG CAATTCTGTC ATCCCAGCAG GCAGAGCGCT GCAGGAGGGC TCAGGCGTTC
    ATCACCACGC AGGGCCTGCA GCCCGAAACT GTGGCAGAGC TGGTGGCCAA AGAGGTCGTG
    CAGGAGTTAC TGGCCTCTTC AGAAGGAAAA GGACAGAAGC AGGTTTCCAA CCCTGCAGCT
    GAGAGCCAGG CGTTCCTGCA GCTCGCAAAG CTGTGCCAGG ACCACACGCT GGTGGGCATG
    AAGCTGCTGG AGAAAATTTC CTCTGTGCCA CGTGGAGAAC TGTCATGCAT TACAGAACTG
    CTGATTCTGG CCCATAATTG CTTTAGTCTG ACTTGCCACA TGGAAGGAAT CACCAGAGTC
    CTGCAGGCAG CACGGCTGCT CACAGATGAG CATTTGGCAC CTAATGATGA GTATGGACTT
    ATGGTTCGTC TTCTCACTGG GATCGGCAGA TACAATGAGA TGACCTACAT CTTTGAGCTG
    CTGCACGAGA AGCATTACTT TGAAGTGCTC ATGAGGAAGA AGTTGGACCC GAGTGGGACA
    CTGAAGACAG CTCTGCTGGA TTACATCAAG CGCTGCCGCC CAGGGGACAG TGAGAAGCAC
    AACATGATTG CCCTTTGCTT CAGCATGTGC CGGGAAATTG GAGAGAATCA CGAGGCTGCT
    GCTTGCATAC AGCTGAAACT CATTGAATCT CAGCCATGGG AGGAGTCCCT TCAGGATGTT
    GCAAACTTAA AGAAACTGCT GATGAAAGCT CTGACCCTCT TCATTGATGC AGCAGAAAGT
    TACTCTAAGG CGCTGCGCTG CCGCCGCCTC ACAAAGCTGA TCACTCTGCA GCTGCATTTT
    CTGAGCACCG GTCAGAGCAC GATGCTGATT AACAGGAACA GGCAGAACCT CATGGAATGC
    ATCGTGTCCC TGCCTCGATT CTATCAGGCG GCGGTTGTGG CCGAGGCATA TGAGTTTGTT
    CCAGATTGGG CTGAAGTTCT GTACCAACAA GTGATCGCTA AGGGAGACTT CAGCTACTTA
    GAAGAATTTA AACAGCAGAG ACTGCTGAAG CCTGCCATGT TTGAGGAGAT TGCGAAGAAG
    TGTAAGCAGC ACCCACCCAG CGATGCTGCT CTGAGGAATC TGAAGAAGCT GCTCGCTTAC
    TGCGAGGACA TCTCCACGTA CTACAGGCTG GCATATGATA ACAAGTTCTA TGATGTGGTC
    AATATGCTCC TGAAGGACGC GCAGACTGGA TGCTGCCTTA ATGATATGTT ATCCAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943993.1
    CDS104..7363
    Translation

    Target ORF information:

    RefSeq Version XM_004943936.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943936.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
    GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC
    CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG
    CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT
    GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC
    TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG
    GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG
    AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG
    CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT
    TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT
    TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA
    CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC
    ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT
    TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT
    CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG
    GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC
    AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT
    TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA
    GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT
    TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG
    CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT
    AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA
    CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC
    GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC
    TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC
    AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA
    GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA
    AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA
    GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG
    CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG
    CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA
    GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA
    CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT
    GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT
    GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC
    CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG
    GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG
    GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA
    CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA
    ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA
    GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT
    TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC
    AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC
    CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG
    ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC
    AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG
    TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT
    GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA
    GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT
    GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA
    GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA
    GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT
    CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA
    GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG
    TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG
    AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT
    CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT
    GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC
    CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC
    AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG
    TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG
    ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA
    GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA
    GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG
    CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA
    GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT
    CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC
    TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT
    GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA
    TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG
    TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA
    GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT
    TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT
    GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC
    AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT
    GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG
    GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG
    TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC
    CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG
    GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA
    TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT
    GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC
    TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG
    CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG
    CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA
    GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC
    GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG
    GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT
    ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA
    ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG
    CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC
    TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC
    GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG
    CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT
    TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG
    GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA
    GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA
    GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG
    GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGTTCT GTGTCTGTAT
    GAACTTTCTA AGGAACTGAA CTGCTCCTAC AGTGAGATGT CAGCCCTTGA CCCTGAGAAA
    GTGCTTCGAG CAATTCTGTC ATCCCAGCAG GCAGAGCGCT GCAGGAGGGC TCAGGCGTTC
    ATCACCACGC AGGGCCTGCA GCCCGAAACT GTGGCAGAGC TGGTGGCCAA AGAGGTCGTG
    CAGGAGTTAC TGGCCTCTTC AGAAGGAAAA GGACAGAAGC AGGTTTCCAA CCCTGCAGCT
    GAGAGCCAGG CGTTCCTGCA GCTCGCAAAG CTGTGCCAGG ACCACACGCT GGTGGGCATG
    AAGCTGCTGG AGAAAATTTC CTCTGTGCCA CGTGGAGAAC TGTCATGCAT TACAGAACTG
    CTGATTCTGG CCCATAATTG CTTTAGTCTG ACTTGCCACA TGGAAGGAAT CACCAGAGTC
    CTGCAGGCAG CACGGCTGCT CACAGATGAG CATTTGGCAC CTAATGATGA GTATGGACTT
    ATGGTTCGTC TTCTCACTGG GATCGGCAGA TACAATGAGA TGACCTACAT CTTTGAGCTG
    CTGCACGAGA AGCATTACTT TGAAGTGCTC ATGAGGAAGA AGTTGGACCC GAGTGGGACA
    CTGAAGACAG CTCTGCTGGA TTACATCAAG CGCTGCCGCC CAGGGGACAG TGAGAAGCAC
    AACATGATTG CCCTTTGCTT CAGCATGTGC CGGGAAATTG GAGAGAATCA CGAGGCTGCT
    GCTTGCATAC AGCTGAAACT CATTGAATCT CAGCCATGGG AGGAGTCCCT TCAGGATGTT
    GCAAACTTAA AGAAACTGCT GATGAAAGCT CTGACCCTCT TCATTGATGC AGCAGAAAGT
    TACTCTAAGG CGCTGCGCTG CCGCCGCCTC ACAAAGCTGA TCACTCTGCA GCTGCATTTT
    CTGAGCACCG GTCAGAGCAC GATGCTGATT AACAGGAACA GGCAGAACCT CATGGAATGC
    ATCGTGTCCC TGCCTCGATT CTATCAGGCG GCGGTTGTGG CCGAGGCATA TGAGTTTGTT
    CCAGATTGGG CTGAAGTTCT GTACCAACAA GTGATCGCTA AGGGAGACTT CAGCTACTTA
    GAAGAATTTA AACAGCAGAG ACTGCTGAAG CCTGCCATGT TTGAGGAGAT TGCGAAGAAG
    TGTAAGCAGC ACCCACCCAG CGATGCTGCT CTGAGGAATC TGAAGAAGCT GCTCGCTTAC
    TGCGAGGACA TCTCCACGTA CTACAGGCTG GCATATGATA ACAAGTTCTA TGATGTGGTC
    AATATGCTCC TGAAGGACGC GCAGACTGGA TGCTGCCTTA ATGATATGTT ATCCAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa13355
    Clone ID Related Accession (Same CDS sequence) XM_004943937.2 , XM_004943937.3
    Accession Version XM_004943937.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7251bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product spatacsin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455925.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004943937.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
    GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC
    CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG
    CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT
    GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC
    TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG
    GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG
    AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG
    CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT
    TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT
    TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA
    CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC
    ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT
    TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT
    CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG
    GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC
    AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT
    TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA
    GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT
    TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG
    CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT
    AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA
    CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC
    GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC
    TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC
    AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA
    GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA
    AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA
    GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG
    CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG
    CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA
    GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA
    CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT
    GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT
    GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC
    CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG
    GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG
    GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA
    CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA
    ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA
    GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT
    TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC
    AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC
    CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG
    ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC
    AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG
    TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT
    GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA
    GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT
    GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA
    GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA
    GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT
    CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA
    GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG
    TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG
    AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT
    CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT
    GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC
    CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC
    AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG
    TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG
    ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA
    GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA
    GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG
    CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA
    GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT
    CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC
    TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT
    GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA
    TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG
    TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA
    GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT
    TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT
    GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC
    AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT
    GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG
    GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG
    TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC
    CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG
    GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA
    TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT
    GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC
    TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG
    CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG
    CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA
    GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC
    GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG
    GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT
    ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA
    ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG
    CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC
    TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC
    GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG
    CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT
    TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG
    GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA
    GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA
    GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG
    GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGAACT GAACTGCTCC
    TACAGTGAGA TGTCAGCCCT TGACCCTGAG AAAGTGCTTC GAGCAATTCT GTCATCCCAG
    CAGGCAGAGC GCTGCAGGAG GGCTCAGGCG TTCATCACCA CGCAGGGCCT GCAGCCCGAA
    ACTGTGGCAG AGCTGGTGGC CAAAGAGGTC GTGCAGGAGT TACTGGCCTC TTCAGAAGGA
    AAAGGACAGA AGCAGGTTTC CAACCCTGCA GCTGAGAGCC AGGCGTTCCT GCAGCTCGCA
    AAGCTGTGCC AGGACCACAC GCTGGTGGGC ATGAAGCTGC TGGAGAAAAT TTCCTCTGTG
    CCACGTGGAG AACTGTCATG CATTACAGAA CTGCTGATTC TGGCCCATAA TTGCTTTAGT
    CTGACTTGCC ACATGGAAGG AATCACCAGA GTCCTGCAGG CAGCACGGCT GCTCACAGAT
    GAGCATTTGG CACCTAATGA TGAGTATGGA CTTATGGTTC GTCTTCTCAC TGGGATCGGC
    AGATACAATG AGATGACCTA CATCTTTGAG CTGCTGCACG AGAAGCATTA CTTTGAAGTG
    CTCATGAGGA AGAAGTTGGA CCCGAGTGGG ACACTGAAGA CAGCTCTGCT GGATTACATC
    AAGCGCTGCC GCCCAGGGGA CAGTGAGAAG CACAACATGA TTGCCCTTTG CTTCAGCATG
    TGCCGGGAAA TTGGAGAGAA TCACGAGGCT GCTGCTTGCA TACAGCTGAA ACTCATTGAA
    TCTCAGCCAT GGGAGGAGTC CCTTCAGGAT GTTGCAAACT TAAAGAAACT GCTGATGAAA
    GCTCTGACCC TCTTCATTGA TGCAGCAGAA AGTTACTCTA AGGACTCCTG TGTCCGCCAG
    GCGCTGCGCT GCCGCCGCCT CACAAAGCTG ATCACTCTGC AGCTGCATTT TCTGAGCACC
    GGTCAGAGCA CGATGCTGAT TAACAGGAAC AGGCAGAACC TCATGGAATG CATCGTGTCC
    CTGCCTCGAT TCTATCAGGC GGCGGTTGTG GCCGAGGCAT ATGAGTTTGT TCCAGATTGG
    GCTGAAGTTC TGTACCAACA AGTGATCGCT AAGGGAGACT TCAGCTACTT AGAAGAATTT
    AAACAGCAGA GACTGCTGAA GCCTGCCATG TTTGAGGAGA TTGCGAAGAA GTGTAAGCAG
    CACCCACCCA GCGATGCTGC TCTGAGGAAT CTGAAGAAGC TGCTCGCTTA CTGCGAGGAC
    ATCTCCACGT ACTACAGGCT GGCATATGAT AACAAGTTCT ATGATGTGGT CAATATGCTC
    CTGAAGGACG CGCAGACTGG ATGCTGCCTT AATGATATGT TATCCAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943994.1
    CDS104..7354
    Translation

    Target ORF information:

    RefSeq Version XM_004943937.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943937.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
    GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC
    CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG
    CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT
    GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC
    TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG
    GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG
    AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG
    CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT
    TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT
    TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA
    CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC
    ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT
    TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT
    CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG
    GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC
    AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT
    TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA
    GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT
    TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG
    CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT
    AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA
    CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC
    GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC
    TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC
    AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA
    GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA
    AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA
    GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG
    CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG
    CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA
    GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA
    CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT
    GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT
    GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC
    CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG
    GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG
    GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA
    CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA
    ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA
    GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT
    TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC
    AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC
    CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG
    ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC
    AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG
    TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT
    GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA
    GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT
    GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA
    GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA
    GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT
    CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA
    GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG
    TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG
    AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT
    CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT
    GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC
    CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC
    AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG
    TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG
    ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA
    GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA
    GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG
    CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA
    GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT
    CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC
    TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT
    GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA
    TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG
    TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA
    GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT
    TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT
    GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC
    AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT
    GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG
    GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG
    TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC
    CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG
    GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA
    TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT
    GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC
    TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG
    CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG
    CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA
    GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC
    GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG
    GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT
    ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA
    ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG
    CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC
    TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC
    GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG
    CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT
    TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG
    GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA
    GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA
    GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG
    GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGAACT GAACTGCTCC
    TACAGTGAGA TGTCAGCCCT TGACCCTGAG AAAGTGCTTC GAGCAATTCT GTCATCCCAG
    CAGGCAGAGC GCTGCAGGAG GGCTCAGGCG TTCATCACCA CGCAGGGCCT GCAGCCCGAA
    ACTGTGGCAG AGCTGGTGGC CAAAGAGGTC GTGCAGGAGT TACTGGCCTC TTCAGAAGGA
    AAAGGACAGA AGCAGGTTTC CAACCCTGCA GCTGAGAGCC AGGCGTTCCT GCAGCTCGCA
    AAGCTGTGCC AGGACCACAC GCTGGTGGGC ATGAAGCTGC TGGAGAAAAT TTCCTCTGTG
    CCACGTGGAG AACTGTCATG CATTACAGAA CTGCTGATTC TGGCCCATAA TTGCTTTAGT
    CTGACTTGCC ACATGGAAGG AATCACCAGA GTCCTGCAGG CAGCACGGCT GCTCACAGAT
    GAGCATTTGG CACCTAATGA TGAGTATGGA CTTATGGTTC GTCTTCTCAC TGGGATCGGC
    AGATACAATG AGATGACCTA CATCTTTGAG CTGCTGCACG AGAAGCATTA CTTTGAAGTG
    CTCATGAGGA AGAAGTTGGA CCCGAGTGGG ACACTGAAGA CAGCTCTGCT GGATTACATC
    AAGCGCTGCC GCCCAGGGGA CAGTGAGAAG CACAACATGA TTGCCCTTTG CTTCAGCATG
    TGCCGGGAAA TTGGAGAGAA TCACGAGGCT GCTGCTTGCA TACAGCTGAA ACTCATTGAA
    TCTCAGCCAT GGGAGGAGTC CCTTCAGGAT GTTGCAAACT TAAAGAAACT GCTGATGAAA
    GCTCTGACCC TCTTCATTGA TGCAGCAGAA AGTTACTCTA AGGACTCCTG TGTCCGCCAG
    GCGCTGCGCT GCCGCCGCCT CACAAAGCTG ATCACTCTGC AGCTGCATTT TCTGAGCACC
    GGTCAGAGCA CGATGCTGAT TAACAGGAAC AGGCAGAACC TCATGGAATG CATCGTGTCC
    CTGCCTCGAT TCTATCAGGC GGCGGTTGTG GCCGAGGCAT ATGAGTTTGT TCCAGATTGG
    GCTGAAGTTC TGTACCAACA AGTGATCGCT AAGGGAGACT TCAGCTACTT AGAAGAATTT
    AAACAGCAGA GACTGCTGAA GCCTGCCATG TTTGAGGAGA TTGCGAAGAA GTGTAAGCAG
    CACCCACCCA GCGATGCTGC TCTGAGGAAT CTGAAGAAGC TGCTCGCTTA CTGCGAGGAC
    ATCTCCACGT ACTACAGGCT GGCATATGAT AACAAGTTCT ATGATGTGGT CAATATGCTC
    CTGAAGGACG CGCAGACTGG ATGCTGCCTT AATGATATGT TATCCAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa13355
    Clone ID Related Accession (Same CDS sequence) XM_004943937.2 , XM_004943937.3
    Accession Version XM_004943937.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7251bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product spatacsin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004943937.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
    GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC
    CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG
    CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT
    GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC
    TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG
    GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG
    AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG
    CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT
    TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT
    TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA
    CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC
    ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT
    TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT
    CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG
    GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC
    AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT
    TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA
    GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT
    TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG
    CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT
    AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA
    CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC
    GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC
    TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC
    AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA
    GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA
    AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA
    GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG
    CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG
    CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA
    GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA
    CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT
    GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT
    GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC
    CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG
    GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG
    GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA
    CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA
    ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA
    GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT
    TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC
    AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC
    CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG
    ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC
    AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG
    TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT
    GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA
    GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT
    GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA
    GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA
    GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT
    CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA
    GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG
    TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG
    AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT
    CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT
    GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC
    CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC
    AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG
    TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG
    ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA
    GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA
    GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG
    CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA
    GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT
    CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC
    TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT
    GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA
    TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG
    TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA
    GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT
    TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT
    GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC
    AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT
    GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG
    GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG
    TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC
    CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG
    GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA
    TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT
    GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC
    TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG
    CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG
    CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA
    GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC
    GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG
    GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT
    ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA
    ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG
    CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC
    TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC
    GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG
    CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT
    TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG
    GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA
    GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA
    GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG
    GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGAACT GAACTGCTCC
    TACAGTGAGA TGTCAGCCCT TGACCCTGAG AAAGTGCTTC GAGCAATTCT GTCATCCCAG
    CAGGCAGAGC GCTGCAGGAG GGCTCAGGCG TTCATCACCA CGCAGGGCCT GCAGCCCGAA
    ACTGTGGCAG AGCTGGTGGC CAAAGAGGTC GTGCAGGAGT TACTGGCCTC TTCAGAAGGA
    AAAGGACAGA AGCAGGTTTC CAACCCTGCA GCTGAGAGCC AGGCGTTCCT GCAGCTCGCA
    AAGCTGTGCC AGGACCACAC GCTGGTGGGC ATGAAGCTGC TGGAGAAAAT TTCCTCTGTG
    CCACGTGGAG AACTGTCATG CATTACAGAA CTGCTGATTC TGGCCCATAA TTGCTTTAGT
    CTGACTTGCC ACATGGAAGG AATCACCAGA GTCCTGCAGG CAGCACGGCT GCTCACAGAT
    GAGCATTTGG CACCTAATGA TGAGTATGGA CTTATGGTTC GTCTTCTCAC TGGGATCGGC
    AGATACAATG AGATGACCTA CATCTTTGAG CTGCTGCACG AGAAGCATTA CTTTGAAGTG
    CTCATGAGGA AGAAGTTGGA CCCGAGTGGG ACACTGAAGA CAGCTCTGCT GGATTACATC
    AAGCGCTGCC GCCCAGGGGA CAGTGAGAAG CACAACATGA TTGCCCTTTG CTTCAGCATG
    TGCCGGGAAA TTGGAGAGAA TCACGAGGCT GCTGCTTGCA TACAGCTGAA ACTCATTGAA
    TCTCAGCCAT GGGAGGAGTC CCTTCAGGAT GTTGCAAACT TAAAGAAACT GCTGATGAAA
    GCTCTGACCC TCTTCATTGA TGCAGCAGAA AGTTACTCTA AGGACTCCTG TGTCCGCCAG
    GCGCTGCGCT GCCGCCGCCT CACAAAGCTG ATCACTCTGC AGCTGCATTT TCTGAGCACC
    GGTCAGAGCA CGATGCTGAT TAACAGGAAC AGGCAGAACC TCATGGAATG CATCGTGTCC
    CTGCCTCGAT TCTATCAGGC GGCGGTTGTG GCCGAGGCAT ATGAGTTTGT TCCAGATTGG
    GCTGAAGTTC TGTACCAACA AGTGATCGCT AAGGGAGACT TCAGCTACTT AGAAGAATTT
    AAACAGCAGA GACTGCTGAA GCCTGCCATG TTTGAGGAGA TTGCGAAGAA GTGTAAGCAG
    CACCCACCCA GCGATGCTGC TCTGAGGAAT CTGAAGAAGC TGCTCGCTTA CTGCGAGGAC
    ATCTCCACGT ACTACAGGCT GGCATATGAT AACAAGTTCT ATGATGTGGT CAATATGCTC
    CTGAAGGACG CGCAGACTGG ATGCTGCCTT AATGATATGT TATCCAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943994.1
    CDS143..7393
    Translation

    Target ORF information:

    RefSeq Version XM_004943937.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943937.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
    GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC
    CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG
    CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT
    GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC
    TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG
    GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG
    AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG
    CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT
    TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT
    TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA
    CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC
    ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT
    TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT
    CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG
    GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC
    AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT
    TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA
    GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT
    TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG
    CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT
    AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA
    CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC
    GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC
    TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC
    AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA
    GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA
    AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA
    GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG
    CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG
    CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA
    GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA
    CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT
    GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT
    GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC
    CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG
    GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG
    GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA
    CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA
    ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA
    GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT
    TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC
    AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC
    CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG
    ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC
    AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG
    TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT
    GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA
    GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT
    GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA
    GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA
    GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT
    CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA
    GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG
    TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG
    AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT
    CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT
    GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC
    CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC
    AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG
    TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG
    ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA
    GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA
    GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG
    CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA
    GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT
    CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC
    TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT
    GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA
    TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG
    TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA
    GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT
    TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT
    GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC
    AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT
    GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG
    GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG
    TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC
    CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG
    GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA
    TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT
    GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC
    TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG
    CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG
    CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA
    GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC
    GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG
    GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT
    ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA
    ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG
    CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC
    TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC
    GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG
    CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT
    TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG
    GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA
    GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA
    GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG
    GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGAACT GAACTGCTCC
    TACAGTGAGA TGTCAGCCCT TGACCCTGAG AAAGTGCTTC GAGCAATTCT GTCATCCCAG
    CAGGCAGAGC GCTGCAGGAG GGCTCAGGCG TTCATCACCA CGCAGGGCCT GCAGCCCGAA
    ACTGTGGCAG AGCTGGTGGC CAAAGAGGTC GTGCAGGAGT TACTGGCCTC TTCAGAAGGA
    AAAGGACAGA AGCAGGTTTC CAACCCTGCA GCTGAGAGCC AGGCGTTCCT GCAGCTCGCA
    AAGCTGTGCC AGGACCACAC GCTGGTGGGC ATGAAGCTGC TGGAGAAAAT TTCCTCTGTG
    CCACGTGGAG AACTGTCATG CATTACAGAA CTGCTGATTC TGGCCCATAA TTGCTTTAGT
    CTGACTTGCC ACATGGAAGG AATCACCAGA GTCCTGCAGG CAGCACGGCT GCTCACAGAT
    GAGCATTTGG CACCTAATGA TGAGTATGGA CTTATGGTTC GTCTTCTCAC TGGGATCGGC
    AGATACAATG AGATGACCTA CATCTTTGAG CTGCTGCACG AGAAGCATTA CTTTGAAGTG
    CTCATGAGGA AGAAGTTGGA CCCGAGTGGG ACACTGAAGA CAGCTCTGCT GGATTACATC
    AAGCGCTGCC GCCCAGGGGA CAGTGAGAAG CACAACATGA TTGCCCTTTG CTTCAGCATG
    TGCCGGGAAA TTGGAGAGAA TCACGAGGCT GCTGCTTGCA TACAGCTGAA ACTCATTGAA
    TCTCAGCCAT GGGAGGAGTC CCTTCAGGAT GTTGCAAACT TAAAGAAACT GCTGATGAAA
    GCTCTGACCC TCTTCATTGA TGCAGCAGAA AGTTACTCTA AGGACTCCTG TGTCCGCCAG
    GCGCTGCGCT GCCGCCGCCT CACAAAGCTG ATCACTCTGC AGCTGCATTT TCTGAGCACC
    GGTCAGAGCA CGATGCTGAT TAACAGGAAC AGGCAGAACC TCATGGAATG CATCGTGTCC
    CTGCCTCGAT TCTATCAGGC GGCGGTTGTG GCCGAGGCAT ATGAGTTTGT TCCAGATTGG
    GCTGAAGTTC TGTACCAACA AGTGATCGCT AAGGGAGACT TCAGCTACTT AGAAGAATTT
    AAACAGCAGA GACTGCTGAA GCCTGCCATG TTTGAGGAGA TTGCGAAGAA GTGTAAGCAG
    CACCCACCCA GCGATGCTGC TCTGAGGAAT CTGAAGAAGC TGCTCGCTTA CTGCGAGGAC
    ATCTCCACGT ACTACAGGCT GGCATATGAT AACAAGTTCT ATGATGTGGT CAATATGCTC
    CTGAAGGACG CGCAGACTGG ATGCTGCCTT AATGATATGT TATCCAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa13357
    Clone ID Related Accession (Same CDS sequence) XM_004943939.2 , XM_004943939.3
    Accession Version XM_004943939.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6042bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product spatacsin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455925.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004943939.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
    GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC
    CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG
    CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT
    GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC
    TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG
    GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG
    AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG
    CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT
    TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT
    TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA
    CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC
    ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT
    TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT
    CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG
    GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC
    AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT
    TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA
    GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT
    TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG
    CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT
    AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA
    CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC
    GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC
    TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC
    AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA
    GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA
    AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA
    GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG
    CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG
    CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA
    GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA
    CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT
    GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT
    GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC
    CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG
    GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG
    GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA
    CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA
    ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA
    GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT
    TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC
    AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC
    CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG
    ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC
    AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG
    TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT
    GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA
    GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT
    GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA
    GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA
    GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT
    CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA
    GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG
    TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG
    AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT
    CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT
    GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC
    CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC
    AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG
    TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG
    ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA
    GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA
    GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG
    CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA
    GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT
    CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC
    TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT
    GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA
    TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG
    TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA
    GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT
    TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT
    GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC
    AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT
    GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG
    GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG
    TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC
    CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG
    GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA
    TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT
    GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC
    TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG
    CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG
    CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA
    GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC
    GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG
    GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT
    ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA
    ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG
    CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC
    TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC
    GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG
    CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT
    TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG
    GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA
    GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA
    GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG
    GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGTTCT GTGTCTGTAT
    GAACTTTCTA AGGTGCCAGA AACAAGTTGC AGAGAGCAGT ACTACCAGCC CTCGTGTGGA
    AACATGGCCA GATGCTCCTC AACTCCCCAG GCCCCTACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943996.1
    CDS104..6145
    Translation

    Target ORF information:

    RefSeq Version XM_004943939.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943939.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
    GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC
    CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG
    CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT
    GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC
    TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG
    GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG
    AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG
    CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT
    TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT
    TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA
    CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC
    ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT
    TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT
    CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG
    GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC
    AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT
    TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA
    GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT
    TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG
    CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT
    AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA
    CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC
    GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC
    TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC
    AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA
    GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA
    AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA
    GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG
    CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG
    CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA
    GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA
    CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT
    GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT
    GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC
    CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG
    GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG
    GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA
    CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA
    ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA
    GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT
    TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC
    AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC
    CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG
    ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC
    AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG
    TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT
    GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA
    GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT
    GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA
    GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA
    GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT
    CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA
    GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG
    TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG
    AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT
    CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT
    GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC
    CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC
    AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG
    TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG
    ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA
    GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA
    GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG
    CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA
    GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT
    CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC
    TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT
    GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA
    TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG
    TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA
    GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT
    TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT
    GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC
    AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT
    GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG
    GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG
    TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC
    CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG
    GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA
    TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT
    GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC
    TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG
    CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG
    CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA
    GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC
    GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG
    GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT
    ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA
    ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG
    CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC
    TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC
    GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG
    CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT
    TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG
    GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA
    GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA
    GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG
    GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGTTCT GTGTCTGTAT
    GAACTTTCTA AGGTGCCAGA AACAAGTTGC AGAGAGCAGT ACTACCAGCC CTCGTGTGGA
    AACATGGCCA GATGCTCCTC AACTCCCCAG GCCCCTACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa13357
    Clone ID Related Accession (Same CDS sequence) XM_004943939.2 , XM_004943939.3
    Accession Version XM_004943939.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6042bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product spatacsin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004943939.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
    GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC
    CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG
    CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT
    GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC
    TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG
    GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG
    AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG
    CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT
    TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT
    TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA
    CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC
    ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT
    TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT
    CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG
    GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC
    AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT
    TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA
    GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT
    TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG
    CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT
    AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA
    CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC
    GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC
    TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC
    AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA
    GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA
    AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA
    GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG
    CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG
    CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA
    GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA
    CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT
    GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT
    GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC
    CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG
    GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG
    GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA
    CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA
    ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA
    GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT
    TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC
    AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC
    CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG
    ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC
    AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG
    TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT
    GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA
    GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT
    GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA
    GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA
    GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT
    CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA
    GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG
    TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG
    AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT
    CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT
    GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC
    CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC
    AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG
    TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG
    ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA
    GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA
    GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG
    CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA
    GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT
    CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC
    TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT
    GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA
    TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG
    TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA
    GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT
    TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT
    GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC
    AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT
    GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG
    GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG
    TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC
    CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG
    GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA
    TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT
    GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC
    TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG
    CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG
    CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA
    GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC
    GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG
    GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT
    ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA
    ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG
    CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC
    TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC
    GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG
    CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT
    TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG
    GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA
    GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA
    GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG
    GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGTTCT GTGTCTGTAT
    GAACTTTCTA AGGTGCCAGA AACAAGTTGC AGAGAGCAGT ACTACCAGCC CTCGTGTGGA
    AACATGGCCA GATGCTCCTC AACTCCCCAG GCCCCTACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943996.1
    CDS143..6184
    Translation

    Target ORF information:

    RefSeq Version XM_004943939.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943939.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
    GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC
    CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG
    CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT
    GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC
    TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG
    GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG
    AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG
    CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT
    TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT
    TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA
    CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC
    ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT
    TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT
    CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG
    GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC
    AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT
    TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA
    GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT
    TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG
    CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT
    AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA
    CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC
    GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC
    TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC
    AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA
    GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA
    AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA
    GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG
    CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG
    CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA
    GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA
    CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT
    GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT
    GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC
    CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG
    GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG
    GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA
    CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA
    ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA
    GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT
    TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC
    AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC
    CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG
    ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC
    AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG
    TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT
    GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA
    GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT
    GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA
    GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA
    GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT
    CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA
    GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG
    TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG
    AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT
    CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT
    GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC
    CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC
    AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG
    TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG
    ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA
    GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA
    GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG
    CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA
    GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT
    CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC
    TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT
    GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA
    TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG
    TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA
    GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT
    TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT
    GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC
    AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT
    GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG
    GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG
    TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC
    CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG
    GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA
    TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT
    GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC
    TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG
    CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG
    CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA
    GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC
    GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG
    GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT
    ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA
    ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG
    CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC
    TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC
    GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG
    CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT
    TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG
    GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA
    GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA
    GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG
    GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGTTCT GTGTCTGTAT
    GAACTTTCTA AGGTGCCAGA AACAAGTTGC AGAGAGCAGT ACTACCAGCC CTCGTGTGGA
    AACATGGCCA GATGCTCCTC AACTCCCCAG GCCCCTACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.