SPG11 gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_004943939.2	XP_004943996.1	spatacsin isoform X4
XM_025153966.1	XP_025009734.1	spatacsin isoform X4
XM_004943939.3	XP_004943996.1	spatacsin isoform X6
XM_004943937.2	XP_004943994.1	spatacsin isoform X3
XM_004943936.3	XP_004943993.1	spatacsin isoform X2
XM_025153967.1	XP_025009735.1	spatacsin isoform X5
XM_003641831.3	XP_003641879.2	spatacsin isoform X1
XM_004943936.2	XP_004943993.1	spatacsin isoform X2
XM_003641831.4	XP_003641879.2	spatacsin isoform X1
XM_004943937.3	XP_004943994.1	spatacsin isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa143468	XM_025153966.1 Latest version!	Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa143469	XM_025153967.1 Latest version!	Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa13353	XM_003641831.4 Latest version!	Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OGa13354	XM_004943936.3 Latest version!	Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OGa13355	XM_004943937.3 Latest version!	Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OGa13357	XM_004943939.3 Latest version!	Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
View Old Accession Versions >>
OGa13357	XM_004943939.2	Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OGa13354	XM_004943936.2	Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OGa13353	XM_003641831.3	Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OGa13355	XM_004943937.2	Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
Hide Old Accession Versions >>

SPG11 ( XM_025153966.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025009734.1 Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OGa143468
Clone ID Related Accession (Same CDS sequence)	XM_025153966.1
Accession Version	XM_025153966.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	spatacsin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC 
CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG 
CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT 
GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC 
TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG 
GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG 
AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG 
CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT 
TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT 
TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA 
CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC 
ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT 
TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT 
CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG 
GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC 
AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT 
TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA 
GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT 
TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG 
CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT 
AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA 
CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC 
GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC 
TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC 
AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA 
GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA 
AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA 
GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG 
CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG 
CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA 
GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA 
CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT 
GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT 
GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC 
CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG 
GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG 
GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA 
CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA 
ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA 
GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT 
TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC 
AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC 
CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG 
ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC 
AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG 
TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT 
GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA 
GTACTGCAAA ATCCTCACCC TGTTCCAAAT TTAACTCCTT CCAACTGCCC TATTTTGGAT 
GACAAGGAAC TGCATGAAGC ACATCCCTGG TTTGAATTCC TCGTGCAGTG CCGGGGGGTT 
GCCAGTAACC CACGAGATCC AAAGATGATT TTTCAGGCGA GTCTAGCAAA TGCTCAGATC 
TTGATTCCTA GTAATCAAGC CAGCGTGAGT AGCATGCTGC TGGAGGGACG TACACTGTTA 
GCACTTGCTA CCACCATGTA TGCACCTGGA GGCATTGATC AGGTTCTTCA GAATGAAGAT 
ACAGAGAAAC CACTAAAGAA AGTTGATCCT CAGCTCTTAA AAATGGCATT GAGTCCTTAC 
CCCAAACTCA GAGCTGCTCT TTTTCCCCCG TGCACCTCCC ATGGAATTTT GCCACCTGAC 
ATTTCCCTTT ATCATCTTGT TCAGTCATTA GTGCCGTTTG ATCCCAGTAA GTTATTTGGC 
TGGCAGTCAA CAAATACCCT TGCTGTATCA GATGCATCAA GTGACCTTCC TCATTTCTCA 
TCCCCTGAAC TGGTGAACAA ATATGCTGTA ATGGAACGAC TGGACTTTTT GTACTACCTG 
CGCCATGGAC GCCCATCGTT TGCCTTTGGT ACATTTTTGG TCCAGCAGTT GGTCAAGAGC 
AAAAGCCCCA AACAGTTGAT TCAGCAAGCA GGAAATGAAG CCTATCTTCT AGGCCTTTCT 
TTCTTCAATA CGCCTTCAGT AGCAGCTGCC TGTGTGTGCT TTCTGGAGCT GCTTGGGCTC 
AGCAGCCTCA AACTGAGAGT TGATATTAAG GTTGCAAACA TGATATTCAT TTACAAAACC 
AGAAATGAAG AAACTCAGCA CAATCAAATA AGGGAATCTC TGGTTGAACA GCTAATGAGG 
TTGGCTGATG GTGAAAAAGC AGCAACAGAA GAAGTTCTTA AATCCTTAGA AGAAGCCTTT 
TGGGATTCAA TTGAGCATCA AGGAATAAAG AGGACATCCA GTGACTCCAG ACAGCAGTGG 
TCTTTGGTGA TGCAGTTCTG CAAACTCCAT GCCATGAAAC TGAGCACCTC ATACCTAAAG 
GAGTGTGCTA AATCCAATGA CTGGCTGCAG TTCATCATCC AAGCCCAGCT GTACAGCTAC 
AAGCCAGATG AGGTCTCATC TGTCCTTGAA GGCTTCGCTC CCATTATACA GCGTCACCTG 
ATGTTGGCAC TGGAGAAGTC ACCGTTTCTG CACGCTGCTG CGGGGGCTCC TGAGGGCAGC 
ACTGGGAGCA GATGTGCAGA CAGTCTCTTC CAAATCCTTT TCCGGTGTCA GGAGAGCCCC 
AATGCTTCGT GTCACCTTTT GGCTGAAGGA CTGAGAGAAC ATGCTCCCAT TCTGAGTGTT 
TTAGCAGCGT GTTCCCCTGA TGCTGACATT ATTCACTGTC TCTGTGTCTG GATAATTACC 
AGTGTGGATG ATGTTACCAG ATCTGAAGCG ACAAAACATA TTCAGAGCTC AGTAGAAAAT 
CACAAGTGGG ATCTTCATGA CCTGTCGCTT ATCTGGAAAG TGCTTTTAAG AAAGCAAAAA 
TGTCAAACAC TTATAAGGGG ATTCCAGCTC TTCTTAAAGG ATTCCCCTTT ACTGCATATA 
CTGGAGATGT ACGATCTGTG CATGAACTGT AAAAGGTACT ATGAAGCCAA AACCAAACTG 
GACGAGTTTC AGAAACACCT CACAAATCTG AAGACCACCG ATGGAGCGGC AGCTTCAGGA 
GTCCCTGTGC AATGGTTGGA GTCACAGGCC TTGTTTCTTC TTGAACTGAT GATGCAGCAG 
TGTAGGACAG AATATGAATT GGGAAAGCTC TTACAGCTGT TTGCTGCAAC AGACAACCTT 
CTCCTTGATG GCCCTTATGT GAAGAAACTG TGTGCGCTCA GTGAGACCCT GAGGGATTCC 
TCCGTGCCCA TTAATCGCTC CATGCTGACC AGGTACACCC CAGAAACGTT TCAGAAGGAG 
TGCACATCGA TTCTGGAGCA GCTCCAGGAG AAAGGAATGT TCTCTCTGGC TCGGGAGGTG 
GCAGAGCTGG CTGAACTGCC TGCGGACAGC GTGGTTACCC AAGAGATTCT GAGGGATATA 
CGTCTGTTAA GAGACACAGG AGAGTGGGAT CGAGATCAGA TAAGAATTAA GTTCTGGAAA 
AAGTGTAACG ACAGCTTCGT GAAAAATTCC ATCTCCAGCA AAGCTGCATC GGATTTCTTT 
TTGAATCAGG GCAATGTGGT GTCTGAATCC CCAGTTCGTG AGAAGATAAA CAGCATTTCG 
GAGAGACATC TGCTGCTTAC CCTGGCTGGC CATTGGCTCG CCAAGGATGT CTCGGTGGCA 
TTGGACAAAT TGGAGGAAAT AGAGAAACAG ATCTGGATTT GTCGCATCAC ACAACAAATG 
TTATCCACAG ATGAGGGGCC AGGCAAGTCG GGACGCTCCT CCTGTGCTGC AGCGAATGGG 
GATTTTACTT TTGATAACTT GGCCAAGGAG TTTTCTTTTT CAAAGCTGCC TGCTCTGAAC 
ACACCAAACT ACTTAAACGT GGAGGGTCTT GAATTGACGG GGTGTCAGCA GACCTTGGCT 
GCGGAGGAGG AGGAGTCGCT CAGGTTTCTG GTCGGATGCC TTCTCGATGA AGGAAGCGTG 
CACGAGGCCA GCCGCGTTTG TCGCTATTTC CATTTCTACA GCAGAGACGT TTCACTGGTG 
TTGCACTGCA GAGCCCTGGC CGCAGGGGAG GCTGACCTGG ACAGACTGCA CGCAGATATT 
CAGGCTCTTG TAGCAATAGA GAAGACACAG GACAGCAGTG CATCGCAGCA GAGCAGAGTC 
CCGAGCACAT CGAGCTTAGA GGACTGGACA CACATTGCAG ATCCATCGCC GGATGATCAG 
ATTGTCGTCA GTCTGAAGGC CCTCACAGAT GAATGTGTCC ATGGGAGAAA CTACTGCAGA 
CAGGTTCTGT GTCTGTATGA ACTTTCTAAG GAACTGAACT GCTCCTACAG TGAGATGTCA 
GCCCTTGACC CTGAGAAAGT GCTTCGAGCA ATTCTGTCAT CCCAGCAGGC AGAGCGCTGC 
AGGAGGGCTC AGGCGTTCAT CACCACGCAG GGCCTGCAGC CCGAAACTGT GGCAGAGCTG 
GTGGCCAAAG AGGTCGTGCA GGAGTTACTG GCCTCTTCAG AAGGAAAAGG ACAGAAGCAG 
GTTTCCAACC CTGCAGCTGA GAGCCAGGCG TTCCTGCAGC TCGCAAAGCT GTGCCAGGAC 
CACACGCTGG TGGGCATGAA GCTGCTGGAG AAAATTTCCT CTGTGCCACG TGGAGAACTG 
TCATGCATTA CAGAACTGCT GATTCTGGCC CATAATTGCT TTAGTCTGAC TTGCCACATG 
GAAGGAATCA CCAGAGTCCT GCAGGCAGCA CGGCTGCTCA CAGATGAGCA TTTGGCACCT 
AATGATGAGT ATGGACTTAT GGTTCGTCTT CTCACTGGGA TCGGCAGATA CAATGAGATG 
ACCTACATCT TTGAGCTGCT GCACGAGAAG CATTACTTTG AAGTGCTCAT GAGGAAGAAG 
TTGGACCCGA GTGGGACACT GAAGACAGCT CTGCTGGATT ACATCAAGCG CTGCCGCCCA 
GGGGACAGTG AGAAGCACAA CATGATTGCC CTTTGCTTCA GCATGTGCCG GGAAATTGGA 
GAGAATCACG AGGCTGCTGC TTGCATACAG CTGAAACTCA TTGAATCTCA GCCATGGGAG 
GAGTCCCTTC AGGATGTTGC AAACTTAAAG AAACTGCTGA TGAAAGCTCT GACCCTCTTC 
ATTGATGCAG CAGAAAGTTA CTCTAAGGAC TCCTGTGTCC GCCAGGCGCT GCGCTGCCGC 
CGCCTCACAA AGCTGATCAC TCTGCAGCTG CATTTTCTGA GCACCGGTCA GAGCACGATG 
CTGATTAACA GGAACAGGCA GAACCTCATG GAATGCATCG TGTCCCTGCC TCGATTCTAT 
CAGGCGGCGG TTGTGGCCGA GGCATATGAG TTTGTTCCAG ATTGGGCTGA AGTTCTGTAC 
CAACAAGTGA TCGCTAAGGG AGACTTCAGC TACTTAGAAG AATTTAAACA GCAGAGACTG 
CTGAAGCCTG CCATGTTTGA GGAGATTGCG AAGAAGTGTA AGCAGCACCC ACCCAGCGAT 
GCTGCTCTGA GGAATCTGAA GAAGCTGCTC GCTTACTGCG AGGACATCTC CACGTACTAC 
AGGCTGGCAT ATGATAACAA GTTCTATGAT GTGGTCAATA TGCTCCTGAA GGACGCGCAG 
ACTGGATGCT GCCTTAATGA TATGTTATCC AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025009734.1
CDS	143..7318
Translation	MAGARRELRVLLAARPGQARDSVRDAARVRLSAEQDALGSLLLRDGIYLESLGPGGGGRARSGVLPGAWADFVWERTAEYDPQAKRTKILALSESHDLFVYEFTREDGKCGPDPLHSCREGVLKKLLEANSVSLSSILSLRILSFKDNKCTLLLNSFIVMHLTFPGEHSSLETCDCFTLDLPPPVLERVTDINFCRGILFFLDSSGWIYVFDSSDGANLAHINVSLCQGGVQDGDRSPAITSPLTALRVSYDLSVAVIISSSSNACSVDLNVYFRQFPGHLICRRDSESLPLKPPEGVDEDDLASSDYSMELLSSPFRTDRSWKAHLSSLCSTIRKRRSRSPHLLCDMNLPWYQYFTHLEDHDPLIYETSEKALAIAPKAFPRVLSNPSQSAGTKLSDMGQQWKQILPVQLSQEVVKLECKSVTGAKALFVILTDDKALTLALWDFESQDVAHYQFGKNSVYVDCNEEVPLCLLLTECGLSLILFGLTQEEFLNRLMIYGSAGVVDSVCHLNGWGRCSIPVHALEAGLENRQLDTVDLFLKSKESLFNLSALRSEAQQSADGASCSFLKNLQELRPALNLLCSAIRENDMEPQSKHFSEQLLNLTLTFLSKQLEEIFVHTEEPDRYLQKAAEILTDYIIELRTFMKNYPRSQVNTTNVGSDSDEDLPAIEESQMWEKLTPEEVIAEAILSNKMPEAQIFFRMHRHPAQSLQELIRTGLDLVYGHLLKGNTREASALLKNMGFDVKQQLHKICFYTADKHVRDLLVKVLQEENYFSEKEKKMIDFVHQVESLYSESFQENKEMQSLSSVRNWRQEQDSSKHLATLESLMDHDGRRVPSREVRVMLNWAQCWDELLQDMILLPRKQSQGLATCNPEALWMYLTAQHDWLNISSWIEEFEPNHSLCGQSKWPPLAASIVDQHTLCSGYMRNEILNKLARNGIFVLSELEDFELLLQRLVSLGGVLQNPHPVPNLTPSNCPILDDKELHEAHPWFEFLVQCRGVASNPRDPKMIFQASLANAQILIPSNQASVSSMLLEGRTLLALATTMYAPGGIDQVLQNEDTEKPLKKVDPQLLKMALSPYPKLRAALFPPCTSHGILPPDISLYHLVQSLVPFDPSKLFGWQSTNTLAVSDASSDLPHFSSPELVNKYAVMERLDFLYYLRHGRPSFAFGTFLVQQLVKSKSPKQLIQQAGNEAYLLGLSFFNTPSVAAACVCFLELLGLSSLKLRVDIKVANMIFIYKTRNEETQHNQIRESLVEQLMRLADGEKAATEEVLKSLEEAFWDSIEHQGIKRTSSDSRQQWSLVMQFCKLHAMKLSTSYLKECAKSNDWLQFIIQAQLYSYKPDEVSSVLEGFAPIIQRHLMLALEKSPFLHAAAGAPEGSTGSRCADSLFQILFRCQESPNASCHLLAEGLREHAPILSVLAACSPDADIIHCLCVWIITSVDDVTRSEATKHIQSSVENHKWDLHDLSLIWKVLLRKQKCQTLIRGFQLFLKDSPLLHILEMYDLCMNCKRYYEAKTKLDEFQKHLTNLKTTDGAAASGVPVQWLESQALFLLELMMQQCRTEYELGKLLQLFAATDNLLLDGPYVKKLCALSETLRDSSVPINRSMLTRYTPETFQKECTSILEQLQEKGMFSLAREVAELAELPADSVVTQEILRDIRLLRDTGEWDRDQIRIKFWKKCNDSFVKNSISSKAASDFFLNQGNVVSESPVREKINSISERHLLLTLAGHWLAKDVSVALDKLEEIEKQIWICRITQQMLSTDEGPGKSGRSSCAAANGDFTFDNLAKEFSFSKLPALNTPNYLNVEGLELTGCQQTLAAEEEESLRFLVGCLLDEGSVHEASRVCRYFHFYSRDVSLVLHCRALAAGEADLDRLHADIQALVAIEKTQDSSASQQSRVPSTSSLEDWTHIADPSPDDQIVVSLKALTDECVHGRNYCRQVLCLYELSKELNCSYSEMSALDPEKVLRAILSSQQAERCRRAQAFITTQGLQPETVAELVAKEVVQELLASSEGKGQKQVSNPAAESQAFLQLAKLCQDHTLVGMKLLEKISSVPRGELSCITELLILAHNCFSLTCHMEGITRVLQAARLLTDEHLAPNDEYGLMVRLLTGIGRYNEMTYIFELLHEKHYFEVLMRKKLDPSGTLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAACIQLKLIESQPWEESLQDVANLKKLLMKALTLFIDAAESYSKDSCVRQALRCRRLTKLITLQLHFLSTGQSTMLINRNRQNLMECIVSLPRFYQAAVVAEAYEFVPDWAEVLYQQVIAKGDFSYLEEFKQQRLLKPAMFEEIAKKCKQHPPSDAALRNLKKLLAYCEDISTYYRLAYDNKFYDVVNMLLKDAQTGCCLNDMLSS

Target ORF information:

RefSeq Version	XM_025153966.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025153966.1

ORF Insert Sequence:

ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC 
CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG 
CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT 
GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC 
TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG 
GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG 
AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG 
CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT 
TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT 
TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA 
CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC 
ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT 
TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT 
CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG 
GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC 
AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT 
TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA 
GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT 
TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG 
CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT 
AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA 
CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC 
GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC 
TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC 
AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA 
GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA 
AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA 
GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG 
CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG 
CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA 
GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA 
CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT 
GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT 
GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC 
CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG 
GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG 
GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA 
CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA 
ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA 
GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT 
TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC 
AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC 
CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG 
ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC 
AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG 
TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT 
GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA 
GTACTGCAAA ATCCTCACCC TGTTCCAAAT TTAACTCCTT CCAACTGCCC TATTTTGGAT 
GACAAGGAAC TGCATGAAGC ACATCCCTGG TTTGAATTCC TCGTGCAGTG CCGGGGGGTT 
GCCAGTAACC CACGAGATCC AAAGATGATT TTTCAGGCGA GTCTAGCAAA TGCTCAGATC 
TTGATTCCTA GTAATCAAGC CAGCGTGAGT AGCATGCTGC TGGAGGGACG TACACTGTTA 
GCACTTGCTA CCACCATGTA TGCACCTGGA GGCATTGATC AGGTTCTTCA GAATGAAGAT 
ACAGAGAAAC CACTAAAGAA AGTTGATCCT CAGCTCTTAA AAATGGCATT GAGTCCTTAC 
CCCAAACTCA GAGCTGCTCT TTTTCCCCCG TGCACCTCCC ATGGAATTTT GCCACCTGAC 
ATTTCCCTTT ATCATCTTGT TCAGTCATTA GTGCCGTTTG ATCCCAGTAA GTTATTTGGC 
TGGCAGTCAA CAAATACCCT TGCTGTATCA GATGCATCAA GTGACCTTCC TCATTTCTCA 
TCCCCTGAAC TGGTGAACAA ATATGCTGTA ATGGAACGAC TGGACTTTTT GTACTACCTG 
CGCCATGGAC GCCCATCGTT TGCCTTTGGT ACATTTTTGG TCCAGCAGTT GGTCAAGAGC 
AAAAGCCCCA AACAGTTGAT TCAGCAAGCA GGAAATGAAG CCTATCTTCT AGGCCTTTCT 
TTCTTCAATA CGCCTTCAGT AGCAGCTGCC TGTGTGTGCT TTCTGGAGCT GCTTGGGCTC 
AGCAGCCTCA AACTGAGAGT TGATATTAAG GTTGCAAACA TGATATTCAT TTACAAAACC 
AGAAATGAAG AAACTCAGCA CAATCAAATA AGGGAATCTC TGGTTGAACA GCTAATGAGG 
TTGGCTGATG GTGAAAAAGC AGCAACAGAA GAAGTTCTTA AATCCTTAGA AGAAGCCTTT 
TGGGATTCAA TTGAGCATCA AGGAATAAAG AGGACATCCA GTGACTCCAG ACAGCAGTGG 
TCTTTGGTGA TGCAGTTCTG CAAACTCCAT GCCATGAAAC TGAGCACCTC ATACCTAAAG 
GAGTGTGCTA AATCCAATGA CTGGCTGCAG TTCATCATCC AAGCCCAGCT GTACAGCTAC 
AAGCCAGATG AGGTCTCATC TGTCCTTGAA GGCTTCGCTC CCATTATACA GCGTCACCTG 
ATGTTGGCAC TGGAGAAGTC ACCGTTTCTG CACGCTGCTG CGGGGGCTCC TGAGGGCAGC 
ACTGGGAGCA GATGTGCAGA CAGTCTCTTC CAAATCCTTT TCCGGTGTCA GGAGAGCCCC 
AATGCTTCGT GTCACCTTTT GGCTGAAGGA CTGAGAGAAC ATGCTCCCAT TCTGAGTGTT 
TTAGCAGCGT GTTCCCCTGA TGCTGACATT ATTCACTGTC TCTGTGTCTG GATAATTACC 
AGTGTGGATG ATGTTACCAG ATCTGAAGCG ACAAAACATA TTCAGAGCTC AGTAGAAAAT 
CACAAGTGGG ATCTTCATGA CCTGTCGCTT ATCTGGAAAG TGCTTTTAAG AAAGCAAAAA 
TGTCAAACAC TTATAAGGGG ATTCCAGCTC TTCTTAAAGG ATTCCCCTTT ACTGCATATA 
CTGGAGATGT ACGATCTGTG CATGAACTGT AAAAGGTACT ATGAAGCCAA AACCAAACTG 
GACGAGTTTC AGAAACACCT CACAAATCTG AAGACCACCG ATGGAGCGGC AGCTTCAGGA 
GTCCCTGTGC AATGGTTGGA GTCACAGGCC TTGTTTCTTC TTGAACTGAT GATGCAGCAG 
TGTAGGACAG AATATGAATT GGGAAAGCTC TTACAGCTGT TTGCTGCAAC AGACAACCTT 
CTCCTTGATG GCCCTTATGT GAAGAAACTG TGTGCGCTCA GTGAGACCCT GAGGGATTCC 
TCCGTGCCCA TTAATCGCTC CATGCTGACC AGGTACACCC CAGAAACGTT TCAGAAGGAG 
TGCACATCGA TTCTGGAGCA GCTCCAGGAG AAAGGAATGT TCTCTCTGGC TCGGGAGGTG 
GCAGAGCTGG CTGAACTGCC TGCGGACAGC GTGGTTACCC AAGAGATTCT GAGGGATATA 
CGTCTGTTAA GAGACACAGG AGAGTGGGAT CGAGATCAGA TAAGAATTAA GTTCTGGAAA 
AAGTGTAACG ACAGCTTCGT GAAAAATTCC ATCTCCAGCA AAGCTGCATC GGATTTCTTT 
TTGAATCAGG GCAATGTGGT GTCTGAATCC CCAGTTCGTG AGAAGATAAA CAGCATTTCG 
GAGAGACATC TGCTGCTTAC CCTGGCTGGC CATTGGCTCG CCAAGGATGT CTCGGTGGCA 
TTGGACAAAT TGGAGGAAAT AGAGAAACAG ATCTGGATTT GTCGCATCAC ACAACAAATG 
TTATCCACAG ATGAGGGGCC AGGCAAGTCG GGACGCTCCT CCTGTGCTGC AGCGAATGGG 
GATTTTACTT TTGATAACTT GGCCAAGGAG TTTTCTTTTT CAAAGCTGCC TGCTCTGAAC 
ACACCAAACT ACTTAAACGT GGAGGGTCTT GAATTGACGG GGTGTCAGCA GACCTTGGCT 
GCGGAGGAGG AGGAGTCGCT CAGGTTTCTG GTCGGATGCC TTCTCGATGA AGGAAGCGTG 
CACGAGGCCA GCCGCGTTTG TCGCTATTTC CATTTCTACA GCAGAGACGT TTCACTGGTG 
TTGCACTGCA GAGCCCTGGC CGCAGGGGAG GCTGACCTGG ACAGACTGCA CGCAGATATT 
CAGGCTCTTG TAGCAATAGA GAAGACACAG GACAGCAGTG CATCGCAGCA GAGCAGAGTC 
CCGAGCACAT CGAGCTTAGA GGACTGGACA CACATTGCAG ATCCATCGCC GGATGATCAG 
ATTGTCGTCA GTCTGAAGGC CCTCACAGAT GAATGTGTCC ATGGGAGAAA CTACTGCAGA 
CAGGTTCTGT GTCTGTATGA ACTTTCTAAG GAACTGAACT GCTCCTACAG TGAGATGTCA 
GCCCTTGACC CTGAGAAAGT GCTTCGAGCA ATTCTGTCAT CCCAGCAGGC AGAGCGCTGC 
AGGAGGGCTC AGGCGTTCAT CACCACGCAG GGCCTGCAGC CCGAAACTGT GGCAGAGCTG 
GTGGCCAAAG AGGTCGTGCA GGAGTTACTG GCCTCTTCAG AAGGAAAAGG ACAGAAGCAG 
GTTTCCAACC CTGCAGCTGA GAGCCAGGCG TTCCTGCAGC TCGCAAAGCT GTGCCAGGAC 
CACACGCTGG TGGGCATGAA GCTGCTGGAG AAAATTTCCT CTGTGCCACG TGGAGAACTG 
TCATGCATTA CAGAACTGCT GATTCTGGCC CATAATTGCT TTAGTCTGAC TTGCCACATG 
GAAGGAATCA CCAGAGTCCT GCAGGCAGCA CGGCTGCTCA CAGATGAGCA TTTGGCACCT 
AATGATGAGT ATGGACTTAT GGTTCGTCTT CTCACTGGGA TCGGCAGATA CAATGAGATG 
ACCTACATCT TTGAGCTGCT GCACGAGAAG CATTACTTTG AAGTGCTCAT GAGGAAGAAG 
TTGGACCCGA GTGGGACACT GAAGACAGCT CTGCTGGATT ACATCAAGCG CTGCCGCCCA 
GGGGACAGTG AGAAGCACAA CATGATTGCC CTTTGCTTCA GCATGTGCCG GGAAATTGGA 
GAGAATCACG AGGCTGCTGC TTGCATACAG CTGAAACTCA TTGAATCTCA GCCATGGGAG 
GAGTCCCTTC AGGATGTTGC AAACTTAAAG AAACTGCTGA TGAAAGCTCT GACCCTCTTC 
ATTGATGCAG CAGAAAGTTA CTCTAAGGAC TCCTGTGTCC GCCAGGCGCT GCGCTGCCGC 
CGCCTCACAA AGCTGATCAC TCTGCAGCTG CATTTTCTGA GCACCGGTCA GAGCACGATG 
CTGATTAACA GGAACAGGCA GAACCTCATG GAATGCATCG TGTCCCTGCC TCGATTCTAT 
CAGGCGGCGG TTGTGGCCGA GGCATATGAG TTTGTTCCAG ATTGGGCTGA AGTTCTGTAC 
CAACAAGTGA TCGCTAAGGG AGACTTCAGC TACTTAGAAG AATTTAAACA GCAGAGACTG 
CTGAAGCCTG CCATGTTTGA GGAGATTGCG AAGAAGTGTA AGCAGCACCC ACCCAGCGAT 
GCTGCTCTGA GGAATCTGAA GAAGCTGCTC GCTTACTGCG AGGACATCTC CACGTACTAC 
AGGCTGGCAT ATGATAACAA GTTCTATGAT GTGGTCAATA TGCTCCTGAA GGACGCGCAG 
ACTGGATGCT GCCTTAATGA TATGTTATCC AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_025153967.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025009735.1 Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X5, mRNA.

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CloneID	OGa143469
Clone ID Related Accession (Same CDS sequence)	XM_025153967.1
Accession Version	XM_025153967.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	spatacsin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGC TGTCCTCGCC TTTCCGCACA GACAGGTCAT GGAAGGCACA CTTATCCTCC 
CTGTGCAGCA CAATAAGGAA AAGAAGATCA CGTTCCCCAC ATTTGCTATG TGATATGAAC 
TTACCTTGGT ATCAGTATTT TACCCACCTT GAAGATCACG ATCCCTTAAT CTATGAAACC 
TCTGAGAAGG CACTGGCCAT TGCTCCAAAG GCTTTTCCAC GTGTCCTTTC CAACCCATCG 
CAGAGTGCTG GCACAAAGCT CAGTGATATG GGGCAGCAGT GGAAACAAAT CCTCCCCGTA 
CAGTTATCAC AAGAAGTGGT GAAACTGGAG TGTAAATCTG TGACTGGAGC CAAAGCACTG 
TTTGTGATCC TAACAGATGA CAAGGCTTTG ACACTTGCTC TCTGGGATTT CGAGTCCCAG 
GATGTAGCAC ATTACCAGTT TGGCAAAAAC AGCGTTTATG TGGATTGTAA TGAGGAGGTG 
CCATTATGCC TGCTGCTGAC AGAGTGTGGC CTCTCCTTAA TTCTGTTTGG TTTAACTCAA 
GAGGAGTTTT TGAATAGACT GATGATCTAT GGCAGCGCTG GAGTCGTAGA CTCTGTATGT 
CACCTCAATG GATGGGGACG GTGTTCAATT CCAGTACATG CATTGGAGGC TGGCTTGGAA 
AATCGTCAGC TAGACACAGT GGACTTATTT TTAAAAAGCA AAGAAAGTCT TTTCAATCTC 
TCTGCGCTGC GCTCCGAAGC CCAGCAGTCT GCAGATGGTG CATCCTGCTC TTTTCTGAAA 
AATTTGCAAG AGCTGAGACC AGCTTTAAAC TTGCTTTGCT CAGCAATCAG AGAGAACGAC 
ATGGAGCCGC AGAGCAAACA CTTCTCAGAG CAGCTGCTGA ACCTCACGCT GACTTTCCTT 
AGCAAGCAGC TTGAAGAAAT TTTTGTCCAC ACAGAGGAAC CTGATAGATA CCTGCAGAAG 
GCTGCAGAGA TTTTAACTGA TTACATCATT GAACTTAGGA CGTTTATGAA AAACTATCCT 
CGATCACAAG TAAATACCAC AAATGTAGGG AGTGATTCTG ATGAAGACCT GCCTGCAATA 
GAGGAAAGCC AGATGTGGGA AAAACTGACA CCTGAGGAAG TCATTGCTGA AGCCATCTTA 
AGCAACAAAA TGCCAGAGGC CCAGATCTTC TTCCGAATGC ACCGGCATCC TGCTCAGAGC 
CTTCAGGAAC TCATTCGGAC AGGTTTGGAT CTGGTCTATG GCCATCTTCT GAAGGGCAAT 
ACCAGAGAGG CCTCAGCACT TCTGAAGAAC ATGGGTTTTG ATGTGAAGCA GCAGTTGCAT 
AAGATATGTT TCTACACGGC TGATAAACAC GTACGAGATT TGCTGGTCAA AGTTTTACAA 
GAAGAAAATT ATTTTTCTGA AAAAGAGAAG AAAATGATAG ACTTTGTGCA TCAGGTTGAA 
AGCTTGTACT CGGAGTCCTT TCAAGAAAAT AAAGAGATGC AATCTCTTTC CAGTGTAAGG 
AACTGGAGAC AGGAACAAGA CTCTTCCAAA CATTTGGCCA CTCTGGAGTC ATTGATGGAT 
CATGATGGAC GCCGGGTGCC CAGCAGGGAG GTCAGGGTGA TGCTGAACTG GGCTCAGTGC 
TGGGATGAGC TCCTTCAGGA TATGATTCTT CTCCCCAGAA AACAAAGCCA AGGACTTGCC 
ACATGTAACC CTGAGGCTCT TTGGATGTAC CTGACAGCCC AGCATGATTG GCTTAATATC 
AGTTCATGGA TTGAAGAATT TGAGCCCAAC CACAGCTTAT GTGGACAAAG TAAATGGCCA 
CCCCTGGCTG CAAGCATTGT TGACCAACAC ACGTTGTGTA GTGGCTACAT GAGAAATGAA 
ATATTGAACA AGCTGGCCAG AAATGGGATT TTTGTCCTTT CTGAATTGGA AGACTTTGAA 
CTTCTGCTCC AAAGATTGGT TTCCCTTGGA GGAGTACTGC AAAATCCTCA CCCTGTTCCA 
AAGTACAGTG CTGCGAGTGG TTTGGACTTC CATGCTCGTT TTGTCCTTCA CTGCTTAGAA 
CACAATTTGC AGTATATGTT ATACACATAC CTAGAATACT ACAGTTTAAC TCCTTCCAAC 
TGCCCTATTT TGGATGACAA GGAACTGCAT GAAGCACATC CCTGGTTTGA ATTCCTCGTG 
CAGTGCCGGG GGGTTGCCAG TAACCCACGA GATCCAAAGA TGATTTTTCA GGCGAGTCTA 
GCAAATGCTC AGATCTTGAT TCCTAGTAAT CAAGCCAGCG TGAGTAGCAT GCTGCTGGAG 
GGACGTACAC TGTTAGCACT TGCTACCACC ATGTATGCAC CTGGAGGCAT TGATCAGGTT 
CTTCAGAATG AAGATACAGA GAAACCACTA AAGAAAGTTG ATCCTCAGCT CTTAAAAATG 
GCATTGAGTC CTTACCCCAA ACTCAGAGCT GCTCTTTTTC CCCCGTGCAC CTCCCATGGA 
ATTTTGCCAC CTGACATTTC CCTTTATCAT CTTGTTCAGT CATTAGTGCC GTTTGATCCC 
AGTAAGTTAT TTGGCTGGCA GTCAACAAAT ACCCTTGCTG TATCAGATGC ATCAAGTGAC 
CTTCCTCATT TCTCATCCCC TGAACTGGTG AACAAATATG CTGTAATGGA ACGACTGGAC 
TTTTTGTACT ACCTGCGCCA TGGACGCCCA TCGTTTGCCT TTGGTACATT TTTGGTCCAG 
CAGTTGGTCA AGAGCAAAAG CCCCAAACAG TTGATTCAGC AAGCAGGAAA TGAAGCCTAT 
CTTCTAGGCC TTTCTTTCTT CAATACGCCT TCAGTAGCAG CTGCCTGTGT GTGCTTTCTG 
GAGCTGCTTG GGCTCAGCAG CCTCAAACTG AGAGTTGATA TTAAGGTTGC AAACATGATA 
TTCATTTACA AAACCAGAAA TGAAGAAACT CAGCACAATC AAATAAGGGA ATCTCTGGTT 
GAACAGCTAA TGAGGTTGGC TGATGGTGAA AAAGCAGCAA CAGAAGAAGT TCTTAAATCC 
TTAGAAGAAG CCTTTTGGGA TTCAATTGAG CATCAAGGAA TAAAGAGGAC ATCCAGTGAC 
TCCAGACAGC AGTGGTCTTT GGTGATGCAG TTCTGCAAAC TCCATGCCAT GAAACTGAGC 
ACCTCATACC TAAAGGAGTG TGCTAAATCC AATGACTGGC TGCAGTTCAT CATCCAAGCC 
CAGCTGTACA GCTACAAGCC AGATGAGGTC TCATCTGTCC TTGAAGGCTT CGCTCCCATT 
ATACAGCGTC ACCTGATGTT GGCACTGGAG AAGTCACCGT TTCTGCACGC TGCTGCGGGG 
GCTCCTGAGG GCAGCACTGG GAGCAGATGT GCAGACAGTC TCTTCCAAAT CCTTTTCCGG 
TGTCAGGAGA GCCCCAATGC TTCGTGTCAC CTTTTGGCTG AAGGACTGAG AGAACATGCT 
CCCATTCTGA GTGTTTTAGC AGCGTGTTCC CCTGATGCTG ACATTATTCA CTGTCTCTGT 
GTCTGGATAA TTACCAGTGT GGATGATGTT ACCAGATCTG AAGCGACAAA ACATATTCAG 
AGCTCAGTAG AAAATCACAA GTGGGATCTT CATGACCTGT CGCTTATCTG GAAAGTGCTT 
TTAAGAAAGC AAAAATGTCA AACACTTATA AGGGGATTCC AGCTCTTCTT AAAGGATTCC 
CCTTTACTGC ATATACTGGA GATGTACGAT CTGTGCATGA ACTGTAAAAG GTACTATGAA 
GCCAAAACCA AACTGGACGA GTTTCAGAAA CACCTCACAA ATCTGAAGAC CACCGATGGA 
GCGGCAGCTT CAGGAGTCCC TGTGCAATGG TTGGAGTCAC AGGCCTTGTT TCTTCTTGAA 
CTGATGATGC AGCAGTGTAG GACAGAATAT GAATTGGGAA AGCTCTTACA GCTGTTTGCT 
GCAACAGACA ACCTTCTCCT TGATGGCCCT TATGTGAAGA AACTGTGTGC GCTCAGTGAG 
ACCCTGAGGG ATTCCTCCGT GCCCATTAAT CGCTCCATGC TGACCAGGTA CACCCCAGAA 
ACGTTTCAGA AGGAGTGCAC ATCGATTCTG GAGCAGCTCC AGGAGAAAGG AATGTTCTCT 
CTGGCTCGGG AGGTGGCAGA GCTGGCTGAA CTGCCTGCGG ACAGCGTGGT TACCCAAGAG 
ATTCTGAGGG ATATACGTCT GTTAAGAGAC ACAGGAGAGT GGGATCGAGA TCAGATAAGA 
ATTAAGTTCT GGAAAAAGTG TAACGACAGC TTCGTGAAAA ATTCCATCTC CAGCAAAGCT 
GCATCGGATT TCTTTTTGAA TCAGGGCAAT GTGGTGTCTG AATCCCCAGT TCGTGAGAAG 
ATAAACAGCA TTTCGGAGAG ACATCTGCTG CTTACCCTGG CTGGCCATTG GCTCGCCAAG 
GATGTCTCGG TGGCATTGGA CAAATTGGAG GAAATAGAGA AACAGATCTG GATTTGTCGC 
ATCACACAAC AAATGTTATC CACAGATGAG GGGCCAGGCA AGTCGGGACG CTCCTCCTGT 
GCTGCAGCGA ATGGGGATTT TACTTTTGAT AACTTGGCCA AGGAGTTTTC TTTTTCAAAG 
CTGCCTGCTC TGAACACACC AAACTACTTA AACGTGGAGG GTCTTGAATT GACGGGGTGT 
CAGCAGACCT TGGCTGCGGA GGAGGAGGAG TCGCTCAGGT TTCTGGTCGG ATGCCTTCTC 
GATGAAGGAA GCGTGCACGA GGCCAGCCGC GTTTGTCGCT ATTTCCATTT CTACAGCAGA 
GACGTTTCAC TGGTGTTGCA CTGCAGAGCC CTGGCCGCAG GGGAGGCTGA CCTGGACAGA 
CTGCACGCAG ATATTCAGGC TCTTGTAGCA ATAGAGAAGA CACAGGACAG CAGTGCATCG 
CAGCAGAGCA GAGTCCCGAG CACATCGAGC TTAGAGGACT GGACACACAT TGCAGATCCA 
TCGCCGGATG ATCAGATTGT CGTCAGTCTG AAGGCCCTCA CAGATGAATG TGTCCATGGG 
AGAAACTACT GCAGACAGGT TCTGTGTCTG TATGAACTTT CTAAGGAACT GAACTGCTCC 
TACAGTGAGA TGTCAGCCCT TGACCCTGAG AAAGTGCTTC GAGCAATTCT GTCATCCCAG 
CAGGCAGAGC GCTGCAGGAG GGCTCAGGCG TTCATCACCA CGCAGGGCCT GCAGCCCGAA 
ACTGTGGCAG AGCTGGTGGC CAAAGAGGTC GTGCAGGAGT TACTGGCCTC TTCAGAAGGA 
AAAGGACAGA AGCAGGTTTC CAACCCTGCA GCTGAGAGCC AGGCGTTCCT GCAGCTCGCA 
AAGCTGTGCC AGGACCACAC GCTGGTGGGC ATGAAGCTGC TGGAGAAAAT TTCCTCTGTG 
CCACGTGGAG AACTGTCATG CATTACAGAA CTGCTGATTC TGGCCCATAA TTGCTTTAGT 
CTGACTTGCC ACATGGAAGG AATCACCAGA GTCCTGCAGG CAGCACGGCT GCTCACAGAT 
GAGCATTTGG CACCTAATGA TGAGTATGGA CTTATGGTTC GTCTTCTCAC TGGGATCGGC 
AGATACAATG AGATGACCTA CATCTTTGAG CTGCTGCACG AGAAGCATTA CTTTGAAGTG 
CTCATGAGGA AGAAGTTGGA CCCGAGTGGG ACACTGAAGA CAGCTCTGCT GGATTACATC 
AAGCGCTGCC GCCCAGGGGA CAGTGAGAAG CACAACATGA TTGCCCTTTG CTTCAGCATG 
TGCCGGGAAA TTGGAGAGAA TCACGAGGCT GCTGCTTGCA TACAGCTGAA ACTCATTGAA 
TCTCAGCCAT GGGAGGAGTC CCTTCAGGAT GTTGCAAACT TAAAGAAACT GCTGATGAAA 
GCTCTGACCC TCTTCATTGA TGCAGCAGAA AGTTACTCTA AGGACTCCTG TGTCCGCCAG 
GCGCTGCGCT GCCGCCGCCT CACAAAGCTG ATCACTCTGC AGCTGCATTT TCTGAGCACC 
GGTCAGAGCA CGATGCTGAT TAACAGGAAC AGGCAGAACC TCATGGAATG CATCGTGTCC 
CTGCCTCGAT TCTATCAGGC GGCGGTTGTG GCCGAGGCAT ATGAGTTTGT TCCAGATTGG 
GCTGAAGTTC TGTACCAACA AGTGATCGCT AAGGGAGACT TCAGCTACTT AGAAGAATTT 
AAACAGCAGA GACTGCTGAA GCCTGCCATG TTTGAGGAGA TTGCGAAGAA GTGTAAGCAG 
CACCCACCCA GCGATGCTGC TCTGAGGAAT CTGAAGAAGC TGCTCGCTTA CTGCGAGGAC 
ATCTCCACGT ACTACAGGCT GGCATATGAT AACAAGTTCT ATGATGTGGT CAATATGCTC 
CTGAAGGACG CGCAGACTGG ATGCTGCCTT AATGATATGT TATCCAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025009735.1
CDS	244..6594
Translation	MELLSSPFRTDRSWKAHLSSLCSTIRKRRSRSPHLLCDMNLPWYQYFTHLEDHDPLIYETSEKALAIAPKAFPRVLSNPSQSAGTKLSDMGQQWKQILPVQLSQEVVKLECKSVTGAKALFVILTDDKALTLALWDFESQDVAHYQFGKNSVYVDCNEEVPLCLLLTECGLSLILFGLTQEEFLNRLMIYGSAGVVDSVCHLNGWGRCSIPVHALEAGLENRQLDTVDLFLKSKESLFNLSALRSEAQQSADGASCSFLKNLQELRPALNLLCSAIRENDMEPQSKHFSEQLLNLTLTFLSKQLEEIFVHTEEPDRYLQKAAEILTDYIIELRTFMKNYPRSQVNTTNVGSDSDEDLPAIEESQMWEKLTPEEVIAEAILSNKMPEAQIFFRMHRHPAQSLQELIRTGLDLVYGHLLKGNTREASALLKNMGFDVKQQLHKICFYTADKHVRDLLVKVLQEENYFSEKEKKMIDFVHQVESLYSESFQENKEMQSLSSVRNWRQEQDSSKHLATLESLMDHDGRRVPSREVRVMLNWAQCWDELLQDMILLPRKQSQGLATCNPEALWMYLTAQHDWLNISSWIEEFEPNHSLCGQSKWPPLAASIVDQHTLCSGYMRNEILNKLARNGIFVLSELEDFELLLQRLVSLGGVLQNPHPVPKYSAASGLDFHARFVLHCLEHNLQYMLYTYLEYYSLTPSNCPILDDKELHEAHPWFEFLVQCRGVASNPRDPKMIFQASLANAQILIPSNQASVSSMLLEGRTLLALATTMYAPGGIDQVLQNEDTEKPLKKVDPQLLKMALSPYPKLRAALFPPCTSHGILPPDISLYHLVQSLVPFDPSKLFGWQSTNTLAVSDASSDLPHFSSPELVNKYAVMERLDFLYYLRHGRPSFAFGTFLVQQLVKSKSPKQLIQQAGNEAYLLGLSFFNTPSVAAACVCFLELLGLSSLKLRVDIKVANMIFIYKTRNEETQHNQIRESLVEQLMRLADGEKAATEEVLKSLEEAFWDSIEHQGIKRTSSDSRQQWSLVMQFCKLHAMKLSTSYLKECAKSNDWLQFIIQAQLYSYKPDEVSSVLEGFAPIIQRHLMLALEKSPFLHAAAGAPEGSTGSRCADSLFQILFRCQESPNASCHLLAEGLREHAPILSVLAACSPDADIIHCLCVWIITSVDDVTRSEATKHIQSSVENHKWDLHDLSLIWKVLLRKQKCQTLIRGFQLFLKDSPLLHILEMYDLCMNCKRYYEAKTKLDEFQKHLTNLKTTDGAAASGVPVQWLESQALFLLELMMQQCRTEYELGKLLQLFAATDNLLLDGPYVKKLCALSETLRDSSVPINRSMLTRYTPETFQKECTSILEQLQEKGMFSLAREVAELAELPADSVVTQEILRDIRLLRDTGEWDRDQIRIKFWKKCNDSFVKNSISSKAASDFFLNQGNVVSESPVREKINSISERHLLLTLAGHWLAKDVSVALDKLEEIEKQIWICRITQQMLSTDEGPGKSGRSSCAAANGDFTFDNLAKEFSFSKLPALNTPNYLNVEGLELTGCQQTLAAEEEESLRFLVGCLLDEGSVHEASRVCRYFHFYSRDVSLVLHCRALAAGEADLDRLHADIQALVAIEKTQDSSASQQSRVPSTSSLEDWTHIADPSPDDQIVVSLKALTDECVHGRNYCRQVLCLYELSKELNCSYSEMSALDPEKVLRAILSSQQAERCRRAQAFITTQGLQPETVAELVAKEVVQELLASSEGKGQKQVSNPAAESQAFLQLAKLCQDHTLVGMKLLEKISSVPRGELSCITELLILAHNCFSLTCHMEGITRVLQAARLLTDEHLAPNDEYGLMVRLLTGIGRYNEMTYIFELLHEKHYFEVLMRKKLDPSGTLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAACIQLKLIESQPWEESLQDVANLKKLLMKALTLFIDAAESYSKDSCVRQALRCRRLTKLITLQLHFLSTGQSTMLINRNRQNLMECIVSLPRFYQAAVVAEAYEFVPDWAEVLYQQVIAKGDFSYLEEFKQQRLLKPAMFEEIAKKCKQHPPSDAALRNLKKLLAYCEDISTYYRLAYDNKFYDVVNMLLKDAQTGCCLNDMLSS

Target ORF information:

RefSeq Version	XM_025153967.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025153967.1

ORF Insert Sequence:

ATGGAGCTGC TGTCCTCGCC TTTCCGCACA GACAGGTCAT GGAAGGCACA CTTATCCTCC 
CTGTGCAGCA CAATAAGGAA AAGAAGATCA CGTTCCCCAC ATTTGCTATG TGATATGAAC 
TTACCTTGGT ATCAGTATTT TACCCACCTT GAAGATCACG ATCCCTTAAT CTATGAAACC 
TCTGAGAAGG CACTGGCCAT TGCTCCAAAG GCTTTTCCAC GTGTCCTTTC CAACCCATCG 
CAGAGTGCTG GCACAAAGCT CAGTGATATG GGGCAGCAGT GGAAACAAAT CCTCCCCGTA 
CAGTTATCAC AAGAAGTGGT GAAACTGGAG TGTAAATCTG TGACTGGAGC CAAAGCACTG 
TTTGTGATCC TAACAGATGA CAAGGCTTTG ACACTTGCTC TCTGGGATTT CGAGTCCCAG 
GATGTAGCAC ATTACCAGTT TGGCAAAAAC AGCGTTTATG TGGATTGTAA TGAGGAGGTG 
CCATTATGCC TGCTGCTGAC AGAGTGTGGC CTCTCCTTAA TTCTGTTTGG TTTAACTCAA 
GAGGAGTTTT TGAATAGACT GATGATCTAT GGCAGCGCTG GAGTCGTAGA CTCTGTATGT 
CACCTCAATG GATGGGGACG GTGTTCAATT CCAGTACATG CATTGGAGGC TGGCTTGGAA 
AATCGTCAGC TAGACACAGT GGACTTATTT TTAAAAAGCA AAGAAAGTCT TTTCAATCTC 
TCTGCGCTGC GCTCCGAAGC CCAGCAGTCT GCAGATGGTG CATCCTGCTC TTTTCTGAAA 
AATTTGCAAG AGCTGAGACC AGCTTTAAAC TTGCTTTGCT CAGCAATCAG AGAGAACGAC 
ATGGAGCCGC AGAGCAAACA CTTCTCAGAG CAGCTGCTGA ACCTCACGCT GACTTTCCTT 
AGCAAGCAGC TTGAAGAAAT TTTTGTCCAC ACAGAGGAAC CTGATAGATA CCTGCAGAAG 
GCTGCAGAGA TTTTAACTGA TTACATCATT GAACTTAGGA CGTTTATGAA AAACTATCCT 
CGATCACAAG TAAATACCAC AAATGTAGGG AGTGATTCTG ATGAAGACCT GCCTGCAATA 
GAGGAAAGCC AGATGTGGGA AAAACTGACA CCTGAGGAAG TCATTGCTGA AGCCATCTTA 
AGCAACAAAA TGCCAGAGGC CCAGATCTTC TTCCGAATGC ACCGGCATCC TGCTCAGAGC 
CTTCAGGAAC TCATTCGGAC AGGTTTGGAT CTGGTCTATG GCCATCTTCT GAAGGGCAAT 
ACCAGAGAGG CCTCAGCACT TCTGAAGAAC ATGGGTTTTG ATGTGAAGCA GCAGTTGCAT 
AAGATATGTT TCTACACGGC TGATAAACAC GTACGAGATT TGCTGGTCAA AGTTTTACAA 
GAAGAAAATT ATTTTTCTGA AAAAGAGAAG AAAATGATAG ACTTTGTGCA TCAGGTTGAA 
AGCTTGTACT CGGAGTCCTT TCAAGAAAAT AAAGAGATGC AATCTCTTTC CAGTGTAAGG 
AACTGGAGAC AGGAACAAGA CTCTTCCAAA CATTTGGCCA CTCTGGAGTC ATTGATGGAT 
CATGATGGAC GCCGGGTGCC CAGCAGGGAG GTCAGGGTGA TGCTGAACTG GGCTCAGTGC 
TGGGATGAGC TCCTTCAGGA TATGATTCTT CTCCCCAGAA AACAAAGCCA AGGACTTGCC 
ACATGTAACC CTGAGGCTCT TTGGATGTAC CTGACAGCCC AGCATGATTG GCTTAATATC 
AGTTCATGGA TTGAAGAATT TGAGCCCAAC CACAGCTTAT GTGGACAAAG TAAATGGCCA 
CCCCTGGCTG CAAGCATTGT TGACCAACAC ACGTTGTGTA GTGGCTACAT GAGAAATGAA 
ATATTGAACA AGCTGGCCAG AAATGGGATT TTTGTCCTTT CTGAATTGGA AGACTTTGAA 
CTTCTGCTCC AAAGATTGGT TTCCCTTGGA GGAGTACTGC AAAATCCTCA CCCTGTTCCA 
AAGTACAGTG CTGCGAGTGG TTTGGACTTC CATGCTCGTT TTGTCCTTCA CTGCTTAGAA 
CACAATTTGC AGTATATGTT ATACACATAC CTAGAATACT ACAGTTTAAC TCCTTCCAAC 
TGCCCTATTT TGGATGACAA GGAACTGCAT GAAGCACATC CCTGGTTTGA ATTCCTCGTG 
CAGTGCCGGG GGGTTGCCAG TAACCCACGA GATCCAAAGA TGATTTTTCA GGCGAGTCTA 
GCAAATGCTC AGATCTTGAT TCCTAGTAAT CAAGCCAGCG TGAGTAGCAT GCTGCTGGAG 
GGACGTACAC TGTTAGCACT TGCTACCACC ATGTATGCAC CTGGAGGCAT TGATCAGGTT 
CTTCAGAATG AAGATACAGA GAAACCACTA AAGAAAGTTG ATCCTCAGCT CTTAAAAATG 
GCATTGAGTC CTTACCCCAA ACTCAGAGCT GCTCTTTTTC CCCCGTGCAC CTCCCATGGA 
ATTTTGCCAC CTGACATTTC CCTTTATCAT CTTGTTCAGT CATTAGTGCC GTTTGATCCC 
AGTAAGTTAT TTGGCTGGCA GTCAACAAAT ACCCTTGCTG TATCAGATGC ATCAAGTGAC 
CTTCCTCATT TCTCATCCCC TGAACTGGTG AACAAATATG CTGTAATGGA ACGACTGGAC 
TTTTTGTACT ACCTGCGCCA TGGACGCCCA TCGTTTGCCT TTGGTACATT TTTGGTCCAG 
CAGTTGGTCA AGAGCAAAAG CCCCAAACAG TTGATTCAGC AAGCAGGAAA TGAAGCCTAT 
CTTCTAGGCC TTTCTTTCTT CAATACGCCT TCAGTAGCAG CTGCCTGTGT GTGCTTTCTG 
GAGCTGCTTG GGCTCAGCAG CCTCAAACTG AGAGTTGATA TTAAGGTTGC AAACATGATA 
TTCATTTACA AAACCAGAAA TGAAGAAACT CAGCACAATC AAATAAGGGA ATCTCTGGTT 
GAACAGCTAA TGAGGTTGGC TGATGGTGAA AAAGCAGCAA CAGAAGAAGT TCTTAAATCC 
TTAGAAGAAG CCTTTTGGGA TTCAATTGAG CATCAAGGAA TAAAGAGGAC ATCCAGTGAC 
TCCAGACAGC AGTGGTCTTT GGTGATGCAG TTCTGCAAAC TCCATGCCAT GAAACTGAGC 
ACCTCATACC TAAAGGAGTG TGCTAAATCC AATGACTGGC TGCAGTTCAT CATCCAAGCC 
CAGCTGTACA GCTACAAGCC AGATGAGGTC TCATCTGTCC TTGAAGGCTT CGCTCCCATT 
ATACAGCGTC ACCTGATGTT GGCACTGGAG AAGTCACCGT TTCTGCACGC TGCTGCGGGG 
GCTCCTGAGG GCAGCACTGG GAGCAGATGT GCAGACAGTC TCTTCCAAAT CCTTTTCCGG 
TGTCAGGAGA GCCCCAATGC TTCGTGTCAC CTTTTGGCTG AAGGACTGAG AGAACATGCT 
CCCATTCTGA GTGTTTTAGC AGCGTGTTCC CCTGATGCTG ACATTATTCA CTGTCTCTGT 
GTCTGGATAA TTACCAGTGT GGATGATGTT ACCAGATCTG AAGCGACAAA ACATATTCAG 
AGCTCAGTAG AAAATCACAA GTGGGATCTT CATGACCTGT CGCTTATCTG GAAAGTGCTT 
TTAAGAAAGC AAAAATGTCA AACACTTATA AGGGGATTCC AGCTCTTCTT AAAGGATTCC 
CCTTTACTGC ATATACTGGA GATGTACGAT CTGTGCATGA ACTGTAAAAG GTACTATGAA 
GCCAAAACCA AACTGGACGA GTTTCAGAAA CACCTCACAA ATCTGAAGAC CACCGATGGA 
GCGGCAGCTT CAGGAGTCCC TGTGCAATGG TTGGAGTCAC AGGCCTTGTT TCTTCTTGAA 
CTGATGATGC AGCAGTGTAG GACAGAATAT GAATTGGGAA AGCTCTTACA GCTGTTTGCT 
GCAACAGACA ACCTTCTCCT TGATGGCCCT TATGTGAAGA AACTGTGTGC GCTCAGTGAG 
ACCCTGAGGG ATTCCTCCGT GCCCATTAAT CGCTCCATGC TGACCAGGTA CACCCCAGAA 
ACGTTTCAGA AGGAGTGCAC ATCGATTCTG GAGCAGCTCC AGGAGAAAGG AATGTTCTCT 
CTGGCTCGGG AGGTGGCAGA GCTGGCTGAA CTGCCTGCGG ACAGCGTGGT TACCCAAGAG 
ATTCTGAGGG ATATACGTCT GTTAAGAGAC ACAGGAGAGT GGGATCGAGA TCAGATAAGA 
ATTAAGTTCT GGAAAAAGTG TAACGACAGC TTCGTGAAAA ATTCCATCTC CAGCAAAGCT 
GCATCGGATT TCTTTTTGAA TCAGGGCAAT GTGGTGTCTG AATCCCCAGT TCGTGAGAAG 
ATAAACAGCA TTTCGGAGAG ACATCTGCTG CTTACCCTGG CTGGCCATTG GCTCGCCAAG 
GATGTCTCGG TGGCATTGGA CAAATTGGAG GAAATAGAGA AACAGATCTG GATTTGTCGC 
ATCACACAAC AAATGTTATC CACAGATGAG GGGCCAGGCA AGTCGGGACG CTCCTCCTGT 
GCTGCAGCGA ATGGGGATTT TACTTTTGAT AACTTGGCCA AGGAGTTTTC TTTTTCAAAG 
CTGCCTGCTC TGAACACACC AAACTACTTA AACGTGGAGG GTCTTGAATT GACGGGGTGT 
CAGCAGACCT TGGCTGCGGA GGAGGAGGAG TCGCTCAGGT TTCTGGTCGG ATGCCTTCTC 
GATGAAGGAA GCGTGCACGA GGCCAGCCGC GTTTGTCGCT ATTTCCATTT CTACAGCAGA 
GACGTTTCAC TGGTGTTGCA CTGCAGAGCC CTGGCCGCAG GGGAGGCTGA CCTGGACAGA 
CTGCACGCAG ATATTCAGGC TCTTGTAGCA ATAGAGAAGA CACAGGACAG CAGTGCATCG 
CAGCAGAGCA GAGTCCCGAG CACATCGAGC TTAGAGGACT GGACACACAT TGCAGATCCA 
TCGCCGGATG ATCAGATTGT CGTCAGTCTG AAGGCCCTCA CAGATGAATG TGTCCATGGG 
AGAAACTACT GCAGACAGGT TCTGTGTCTG TATGAACTTT CTAAGGAACT GAACTGCTCC 
TACAGTGAGA TGTCAGCCCT TGACCCTGAG AAAGTGCTTC GAGCAATTCT GTCATCCCAG 
CAGGCAGAGC GCTGCAGGAG GGCTCAGGCG TTCATCACCA CGCAGGGCCT GCAGCCCGAA 
ACTGTGGCAG AGCTGGTGGC CAAAGAGGTC GTGCAGGAGT TACTGGCCTC TTCAGAAGGA 
AAAGGACAGA AGCAGGTTTC CAACCCTGCA GCTGAGAGCC AGGCGTTCCT GCAGCTCGCA 
AAGCTGTGCC AGGACCACAC GCTGGTGGGC ATGAAGCTGC TGGAGAAAAT TTCCTCTGTG 
CCACGTGGAG AACTGTCATG CATTACAGAA CTGCTGATTC TGGCCCATAA TTGCTTTAGT 
CTGACTTGCC ACATGGAAGG AATCACCAGA GTCCTGCAGG CAGCACGGCT GCTCACAGAT 
GAGCATTTGG CACCTAATGA TGAGTATGGA CTTATGGTTC GTCTTCTCAC TGGGATCGGC 
AGATACAATG AGATGACCTA CATCTTTGAG CTGCTGCACG AGAAGCATTA CTTTGAAGTG 
CTCATGAGGA AGAAGTTGGA CCCGAGTGGG ACACTGAAGA CAGCTCTGCT GGATTACATC 
AAGCGCTGCC GCCCAGGGGA CAGTGAGAAG CACAACATGA TTGCCCTTTG CTTCAGCATG 
TGCCGGGAAA TTGGAGAGAA TCACGAGGCT GCTGCTTGCA TACAGCTGAA ACTCATTGAA 
TCTCAGCCAT GGGAGGAGTC CCTTCAGGAT GTTGCAAACT TAAAGAAACT GCTGATGAAA 
GCTCTGACCC TCTTCATTGA TGCAGCAGAA AGTTACTCTA AGGACTCCTG TGTCCGCCAG 
GCGCTGCGCT GCCGCCGCCT CACAAAGCTG ATCACTCTGC AGCTGCATTT TCTGAGCACC 
GGTCAGAGCA CGATGCTGAT TAACAGGAAC AGGCAGAACC TCATGGAATG CATCGTGTCC 
CTGCCTCGAT TCTATCAGGC GGCGGTTGTG GCCGAGGCAT ATGAGTTTGT TCCAGATTGG 
GCTGAAGTTC TGTACCAACA AGTGATCGCT AAGGGAGACT TCAGCTACTT AGAAGAATTT 
AAACAGCAGA GACTGCTGAA GCCTGCCATG TTTGAGGAGA TTGCGAAGAA GTGTAAGCAG 
CACCCACCCA GCGATGCTGC TCTGAGGAAT CTGAAGAAGC TGCTCGCTTA CTGCGAGGAC 
ATCTCCACGT ACTACAGGCT GGCATATGAT AACAAGTTCT ATGATGTGGT CAATATGCTC 
CTGAAGGACG CGCAGACTGG ATGCTGCCTT AATGATATGT TATCCAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_003641831.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_003641879.2 Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X1, mRNA.

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CloneID	OGa13353
Clone ID Related Accession (Same CDS sequence)	XM_003641831.3 , XM_003641831.4
Accession Version	XM_003641831.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	spatacsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455925.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_003641831.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC 
CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG 
CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT 
GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC 
TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG 
GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG 
AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG 
CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT 
TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT 
TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA 
CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC 
ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT 
TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT 
CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG 
GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC 
AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT 
TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA 
GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT 
TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG 
CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT 
AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA 
CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC 
GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC 
TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC 
AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA 
GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA 
AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA 
GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG 
CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG 
CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA 
GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA 
CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT 
GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT 
GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC 
CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG 
GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG 
GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA 
CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA 
ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA 
GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT 
TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC 
AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC 
CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG 
ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC 
AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG 
TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT 
GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA 
GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT 
GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA 
GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA 
GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT 
CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA 
GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG 
TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG 
AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT 
CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT 
GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC 
CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC 
AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG 
TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG 
ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA 
GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA 
GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG 
CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA 
GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT 
CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC 
TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT 
GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA 
TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG 
TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA 
GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT 
TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT 
GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC 
AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT 
GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG 
GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG 
TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC 
CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG 
GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA 
TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT 
GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC 
TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG 
CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG 
CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA 
GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC 
GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG 
GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT 
ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA 
ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG 
CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC 
TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC 
GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG 
CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT 
TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG 
GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA 
GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA 
GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG 
GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGTTCT GTGTCTGTAT 
GAACTTTCTA AGGAACTGAA CTGCTCCTAC AGTGAGATGT CAGCCCTTGA CCCTGAGAAA 
GTGCTTCGAG CAATTCTGTC ATCCCAGCAG GCAGAGCGCT GCAGGAGGGC TCAGGCGTTC 
ATCACCACGC AGGGCCTGCA GCCCGAAACT GTGGCAGAGC TGGTGGCCAA AGAGGTCGTG 
CAGGAGTTAC TGGCCTCTTC AGAAGGAAAA GGACAGAAGC AGGTTTCCAA CCCTGCAGCT 
GAGAGCCAGG CGTTCCTGCA GCTCGCAAAG CTGTGCCAGG ACCACACGCT GGTGGGCATG 
AAGCTGCTGG AGAAAATTTC CTCTGTGCCA CGTGGAGAAC TGTCATGCAT TACAGAACTG 
CTGATTCTGG CCCATAATTG CTTTAGTCTG ACTTGCCACA TGGAAGGAAT CACCAGAGTC 
CTGCAGGCAG CACGGCTGCT CACAGATGAG CATTTGGCAC CTAATGATGA GTATGGACTT 
ATGGTTCGTC TTCTCACTGG GATCGGCAGA TACAATGAGA TGACCTACAT CTTTGAGCTG 
CTGCACGAGA AGCATTACTT TGAAGTGCTC ATGAGGAAGA AGTTGGACCC GAGTGGGACA 
CTGAAGACAG CTCTGCTGGA TTACATCAAG CGCTGCCGCC CAGGGGACAG TGAGAAGCAC 
AACATGATTG CCCTTTGCTT CAGCATGTGC CGGGAAATTG GAGAGAATCA CGAGGCTGCT 
GCTTGCATAC AGCTGAAACT CATTGAATCT CAGCCATGGG AGGAGTCCCT TCAGGATGTT 
GCAAACTTAA AGAAACTGCT GATGAAAGCT CTGACCCTCT TCATTGATGC AGCAGAAAGT 
TACTCTAAGG ACTCCTGTGT CCGCCAGGCG CTGCGCTGCC GCCGCCTCAC AAAGCTGATC 
ACTCTGCAGC TGCATTTTCT GAGCACCGGT CAGAGCACGA TGCTGATTAA CAGGAACAGG 
CAGAACCTCA TGGAATGCAT CGTGTCCCTG CCTCGATTCT ATCAGGCGGC GGTTGTGGCC 
GAGGCATATG AGTTTGTTCC AGATTGGGCT GAAGTTCTGT ACCAACAAGT GATCGCTAAG 
GGAGACTTCA GCTACTTAGA AGAATTTAAA CAGCAGAGAC TGCTGAAGCC TGCCATGTTT 
GAGGAGATTG CGAAGAAGTG TAAGCAGCAC CCACCCAGCG ATGCTGCTCT GAGGAATCTG 
AAGAAGCTGC TCGCTTACTG CGAGGACATC TCCACGTACT ACAGGCTGGC ATATGATAAC 
AAGTTCTATG ATGTGGTCAA TATGCTCCTG AAGGACGCGC AGACTGGATG CTGCCTTAAT 
GATATGTTAT CCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003641879.2
CDS	104..7381
Translation	MAGARRELRVLLAARPGQARDSVRDAARVRLSAEQDALGSLLLRDGIYLESLGPGGGGRARSGVLPGAWADFVWERTAEYDPQAKRTKILALSESHDLFVYEFTREDGKCGPDPLHSCREGVLKKLLEANSVSLSSILSLRILSFKDNKCTLLLNSFIVMHLTFPGEHSSLETCDCFTLDLPPPVLERVTDINFCRGILFFLDSSGWIYVFDSSDGANLAHINVSLCQGGVQDGDRSPAITSPLTALRVSYDLSVAVIISSSSNACSVDLNVYFRQFPGHLICRRDSESLPLKPPEGVDEDDLASSDYSMELLSSPFRTDRSWKAHLSSLCSTIRKRRSRSPHLLCDMNLPWYQYFTHLEDHDPLIYETSEKALAIAPKAFPRVLSNPSQSAGTKLSDMGQQWKQILPVQLSQEVVKLECKSVTGAKALFVILTDDKALTLALWDFESQDVAHYQFGKNSVYVDCNEEVPLCLLLTECGLSLILFGLTQEEFLNRLMIYGSAGVVDSVCHLNGWGRCSIPVHALEAGLENRQLDTVDLFLKSKESLFNLSALRSEAQQSADGASCSFLKNLQELRPALNLLCSAIRENDMEPQSKHFSEQLLNLTLTFLSKQLEEIFVHTEEPDRYLQKAAEILTDYIIELRTFMKNYPRSQVNTTNVGSDSDEDLPAIEESQMWEKLTPEEVIAEAILSNKMPEAQIFFRMHRHPAQSLQELIRTGLDLVYGHLLKGNTREASALLKNMGFDVKQQLHKICFYTADKHVRDLLVKVLQEENYFSEKEKKMIDFVHQVESLYSESFQENKEMQSLSSVRNWRQEQDSSKHLATLESLMDHDGRRVPSREVRVMLNWAQCWDELLQDMILLPRKQSQGLATCNPEALWMYLTAQHDWLNISSWIEEFEPNHSLCGQSKWPPLAASIVDQHTLCSGYMRNEILNKLARNGIFVLSELEDFELLLQRLVSLGGVLQNPHPVPKYSAASGLDFHARFVLHCLEHNLQYMLYTYLEYYSLTPSNCPILDDKELHEAHPWFEFLVQCRGVASNPRDPKMIFQASLANAQILIPSNQASVSSMLLEGRTLLALATTMYAPGGIDQVLQNEDTEKPLKKVDPQLLKMALSPYPKLRAALFPPCTSHGILPPDISLYHLVQSLVPFDPSKLFGWQSTNTLAVSDASSDLPHFSSPELVNKYAVMERLDFLYYLRHGRPSFAFGTFLVQQLVKSKSPKQLIQQAGNEAYLLGLSFFNTPSVAAACVCFLELLGLSSLKLRVDIKVANMIFIYKTRNEETQHNQIRESLVEQLMRLADGEKAATEEVLKSLEEAFWDSIEHQGIKRTSSDSRQQWSLVMQFCKLHAMKLSTSYLKECAKSNDWLQFIIQAQLYSYKPDEVSSVLEGFAPIIQRHLMLALEKSPFLHAAAGAPEGSTGSRCADSLFQILFRCQESPNASCHLLAEGLREHAPILSVLAACSPDADIIHCLCVWIITSVDDVTRSEATKHIQSSVENHKWDLHDLSLIWKVLLRKQKCQTLIRGFQLFLKDSPLLHILEMYDLCMNCKRYYEAKTKLDEFQKHLTNLKTTDGAAASGVPVQWLESQALFLLELMMQQCRTEYELGKLLQLFAATDNLLLDGPYVKKLCALSETLRDSSVPINRSMLTRYTPETFQKECTSILEQLQEKGMFSLAREVAELAELPADSVVTQEILRDIRLLRDTGEWDRDQIRIKFWKKCNDSFVKNSISSKAASDFFLNQGNVVSESPVREKINSISERHLLLTLAGHWLAKDVSVALDKLEEIEKQIWICRITQQMLSTDEGPGKSGRSSCAAANGDFTFDNLAKEFSFSKLPALNTPNYLNVEGLELTGCQQTLAAEEEESLRFLVGCLLDEGSVHEASRVCRYFHFYSRDVSLVLHCRALAAGEADLDRLHADIQALVAIEKTQDSSASQQSRVPSTSSLEDWTHIADPSPDDQIVVSLKALTDECVHGRNYCRQVLCLYELSKELNCSYSEMSALDPEKVLRAILSSQQAERCRRAQAFITTQGLQPETVAELVAKEVVQELLASSEGKGQKQVSNPAAESQAFLQLAKLCQDHTLVGMKLLEKISSVPRGELSCITELLILAHNCFSLTCHMEGITRVLQAARLLTDEHLAPNDEYGLMVRLLTGIGRYNEMTYIFELLHEKHYFEVLMRKKLDPSGTLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAACIQLKLIESQPWEESLQDVANLKKLLMKALTLFIDAAESYSKDSCVRQALRCRRLTKLITLQLHFLSTGQSTMLINRNRQNLMECIVSLPRFYQAAVVAEAYEFVPDWAEVLYQQVIAKGDFSYLEEFKQQRLLKPAMFEEIAKKCKQHPPSDAALRNLKKLLAYCEDISTYYRLAYDNKFYDVVNMLLKDAQTGCCLNDMLSS

Target ORF information:

RefSeq Version	XM_003641831.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003641831.3

ORF Insert Sequence:

ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC 
CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG 
CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT 
GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC 
TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG 
GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG 
AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG 
CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT 
TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT 
TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA 
CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC 
ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT 
TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT 
CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG 
GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC 
AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT 
TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA 
GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT 
TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG 
CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT 
AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA 
CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC 
GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC 
TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC 
AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA 
GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA 
AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA 
GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG 
CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG 
CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA 
GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA 
CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT 
GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT 
GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC 
CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG 
GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG 
GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA 
CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA 
ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA 
GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT 
TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC 
AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC 
CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG 
ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC 
AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG 
TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT 
GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA 
GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT 
GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA 
GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA 
GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT 
CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA 
GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG 
TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG 
AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT 
CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT 
GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC 
CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC 
AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG 
TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG 
ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA 
GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA 
GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG 
CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA 
GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT 
CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC 
TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT 
GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA 
TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG 
TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA 
GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT 
TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT 
GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC 
AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT 
GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG 
GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG 
TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC 
CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG 
GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA 
TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT 
GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC 
TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG 
CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG 
CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA 
GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC 
GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG 
GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT 
ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA 
ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG 
CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC 
TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC 
GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG 
CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT 
TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG 
GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA 
GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA 
GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG 
GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGTTCT GTGTCTGTAT 
GAACTTTCTA AGGAACTGAA CTGCTCCTAC AGTGAGATGT CAGCCCTTGA CCCTGAGAAA 
GTGCTTCGAG CAATTCTGTC ATCCCAGCAG GCAGAGCGCT GCAGGAGGGC TCAGGCGTTC 
ATCACCACGC AGGGCCTGCA GCCCGAAACT GTGGCAGAGC TGGTGGCCAA AGAGGTCGTG 
CAGGAGTTAC TGGCCTCTTC AGAAGGAAAA GGACAGAAGC AGGTTTCCAA CCCTGCAGCT 
GAGAGCCAGG CGTTCCTGCA GCTCGCAAAG CTGTGCCAGG ACCACACGCT GGTGGGCATG 
AAGCTGCTGG AGAAAATTTC CTCTGTGCCA CGTGGAGAAC TGTCATGCAT TACAGAACTG 
CTGATTCTGG CCCATAATTG CTTTAGTCTG ACTTGCCACA TGGAAGGAAT CACCAGAGTC 
CTGCAGGCAG CACGGCTGCT CACAGATGAG CATTTGGCAC CTAATGATGA GTATGGACTT 
ATGGTTCGTC TTCTCACTGG GATCGGCAGA TACAATGAGA TGACCTACAT CTTTGAGCTG 
CTGCACGAGA AGCATTACTT TGAAGTGCTC ATGAGGAAGA AGTTGGACCC GAGTGGGACA 
CTGAAGACAG CTCTGCTGGA TTACATCAAG CGCTGCCGCC CAGGGGACAG TGAGAAGCAC 
AACATGATTG CCCTTTGCTT CAGCATGTGC CGGGAAATTG GAGAGAATCA CGAGGCTGCT 
GCTTGCATAC AGCTGAAACT CATTGAATCT CAGCCATGGG AGGAGTCCCT TCAGGATGTT 
GCAAACTTAA AGAAACTGCT GATGAAAGCT CTGACCCTCT TCATTGATGC AGCAGAAAGT 
TACTCTAAGG ACTCCTGTGT CCGCCAGGCG CTGCGCTGCC GCCGCCTCAC AAAGCTGATC 
ACTCTGCAGC TGCATTTTCT GAGCACCGGT CAGAGCACGA TGCTGATTAA CAGGAACAGG 
CAGAACCTCA TGGAATGCAT CGTGTCCCTG CCTCGATTCT ATCAGGCGGC GGTTGTGGCC 
GAGGCATATG AGTTTGTTCC AGATTGGGCT GAAGTTCTGT ACCAACAAGT GATCGCTAAG 
GGAGACTTCA GCTACTTAGA AGAATTTAAA CAGCAGAGAC TGCTGAAGCC TGCCATGTTT 
GAGGAGATTG CGAAGAAGTG TAAGCAGCAC CCACCCAGCG ATGCTGCTCT GAGGAATCTG 
AAGAAGCTGC TCGCTTACTG CGAGGACATC TCCACGTACT ACAGGCTGGC ATATGATAAC 
AAGTTCTATG ATGTGGTCAA TATGCTCCTG AAGGACGCGC AGACTGGATG CTGCCTTAAT 
GATATGTTAT CCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_003641831.4 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_003641879.2 Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X1, mRNA.

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CloneID	OGa13353
Clone ID Related Accession (Same CDS sequence)	XM_003641831.3 , XM_003641831.4
Accession Version	XM_003641831.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	spatacsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_003641831.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC 
CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG 
CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT 
GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC 
TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG 
GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG 
AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG 
CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT 
TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT 
TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA 
CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC 
ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT 
TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT 
CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG 
GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC 
AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT 
TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA 
GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT 
TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG 
CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT 
AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA 
CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC 
GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC 
TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC 
AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA 
GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA 
AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA 
GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG 
CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG 
CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA 
GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA 
CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT 
GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT 
GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC 
CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG 
GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG 
GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA 
CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA 
ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA 
GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT 
TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC 
AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC 
CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG 
ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC 
AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG 
TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT 
GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA 
GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT 
GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA 
GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA 
GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT 
CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA 
GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG 
TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG 
AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT 
CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT 
GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC 
CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC 
AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG 
TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG 
ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA 
GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA 
GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG 
CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA 
GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT 
CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC 
TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT 
GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA 
TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG 
TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA 
GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT 
TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT 
GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC 
AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT 
GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG 
GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG 
TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC 
CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG 
GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA 
TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT 
GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC 
TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG 
CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG 
CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA 
GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC 
GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG 
GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT 
ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA 
ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG 
CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC 
TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC 
GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG 
CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT 
TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG 
GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA 
GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA 
GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG 
GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGTTCT GTGTCTGTAT 
GAACTTTCTA AGGAACTGAA CTGCTCCTAC AGTGAGATGT CAGCCCTTGA CCCTGAGAAA 
GTGCTTCGAG CAATTCTGTC ATCCCAGCAG GCAGAGCGCT GCAGGAGGGC TCAGGCGTTC 
ATCACCACGC AGGGCCTGCA GCCCGAAACT GTGGCAGAGC TGGTGGCCAA AGAGGTCGTG 
CAGGAGTTAC TGGCCTCTTC AGAAGGAAAA GGACAGAAGC AGGTTTCCAA CCCTGCAGCT 
GAGAGCCAGG CGTTCCTGCA GCTCGCAAAG CTGTGCCAGG ACCACACGCT GGTGGGCATG 
AAGCTGCTGG AGAAAATTTC CTCTGTGCCA CGTGGAGAAC TGTCATGCAT TACAGAACTG 
CTGATTCTGG CCCATAATTG CTTTAGTCTG ACTTGCCACA TGGAAGGAAT CACCAGAGTC 
CTGCAGGCAG CACGGCTGCT CACAGATGAG CATTTGGCAC CTAATGATGA GTATGGACTT 
ATGGTTCGTC TTCTCACTGG GATCGGCAGA TACAATGAGA TGACCTACAT CTTTGAGCTG 
CTGCACGAGA AGCATTACTT TGAAGTGCTC ATGAGGAAGA AGTTGGACCC GAGTGGGACA 
CTGAAGACAG CTCTGCTGGA TTACATCAAG CGCTGCCGCC CAGGGGACAG TGAGAAGCAC 
AACATGATTG CCCTTTGCTT CAGCATGTGC CGGGAAATTG GAGAGAATCA CGAGGCTGCT 
GCTTGCATAC AGCTGAAACT CATTGAATCT CAGCCATGGG AGGAGTCCCT TCAGGATGTT 
GCAAACTTAA AGAAACTGCT GATGAAAGCT CTGACCCTCT TCATTGATGC AGCAGAAAGT 
TACTCTAAGG ACTCCTGTGT CCGCCAGGCG CTGCGCTGCC GCCGCCTCAC AAAGCTGATC 
ACTCTGCAGC TGCATTTTCT GAGCACCGGT CAGAGCACGA TGCTGATTAA CAGGAACAGG 
CAGAACCTCA TGGAATGCAT CGTGTCCCTG CCTCGATTCT ATCAGGCGGC GGTTGTGGCC 
GAGGCATATG AGTTTGTTCC AGATTGGGCT GAAGTTCTGT ACCAACAAGT GATCGCTAAG 
GGAGACTTCA GCTACTTAGA AGAATTTAAA CAGCAGAGAC TGCTGAAGCC TGCCATGTTT 
GAGGAGATTG CGAAGAAGTG TAAGCAGCAC CCACCCAGCG ATGCTGCTCT GAGGAATCTG 
AAGAAGCTGC TCGCTTACTG CGAGGACATC TCCACGTACT ACAGGCTGGC ATATGATAAC 
AAGTTCTATG ATGTGGTCAA TATGCTCCTG AAGGACGCGC AGACTGGATG CTGCCTTAAT 
GATATGTTAT CCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003641879.2
CDS	143..7420
Translation	MAGARRELRVLLAARPGQARDSVRDAARVRLSAEQDALGSLLLRDGIYLESLGPGGGGRARSGVLPGAWADFVWERTAEYDPQAKRTKILALSESHDLFVYEFTREDGKCGPDPLHSCREGVLKKLLEANSVSLSSILSLRILSFKDNKCTLLLNSFIVMHLTFPGEHSSLETCDCFTLDLPPPVLERVTDINFCRGILFFLDSSGWIYVFDSSDGANLAHINVSLCQGGVQDGDRSPAITSPLTALRVSYDLSVAVIISSSSNACSVDLNVYFRQFPGHLICRRDSESLPLKPPEGVDEDDLASSDYSMELLSSPFRTDRSWKAHLSSLCSTIRKRRSRSPHLLCDMNLPWYQYFTHLEDHDPLIYETSEKALAIAPKAFPRVLSNPSQSAGTKLSDMGQQWKQILPVQLSQEVVKLECKSVTGAKALFVILTDDKALTLALWDFESQDVAHYQFGKNSVYVDCNEEVPLCLLLTECGLSLILFGLTQEEFLNRLMIYGSAGVVDSVCHLNGWGRCSIPVHALEAGLENRQLDTVDLFLKSKESLFNLSALRSEAQQSADGASCSFLKNLQELRPALNLLCSAIRENDMEPQSKHFSEQLLNLTLTFLSKQLEEIFVHTEEPDRYLQKAAEILTDYIIELRTFMKNYPRSQVNTTNVGSDSDEDLPAIEESQMWEKLTPEEVIAEAILSNKMPEAQIFFRMHRHPAQSLQELIRTGLDLVYGHLLKGNTREASALLKNMGFDVKQQLHKICFYTADKHVRDLLVKVLQEENYFSEKEKKMIDFVHQVESLYSESFQENKEMQSLSSVRNWRQEQDSSKHLATLESLMDHDGRRVPSREVRVMLNWAQCWDELLQDMILLPRKQSQGLATCNPEALWMYLTAQHDWLNISSWIEEFEPNHSLCGQSKWPPLAASIVDQHTLCSGYMRNEILNKLARNGIFVLSELEDFELLLQRLVSLGGVLQNPHPVPKYSAASGLDFHARFVLHCLEHNLQYMLYTYLEYYSLTPSNCPILDDKELHEAHPWFEFLVQCRGVASNPRDPKMIFQASLANAQILIPSNQASVSSMLLEGRTLLALATTMYAPGGIDQVLQNEDTEKPLKKVDPQLLKMALSPYPKLRAALFPPCTSHGILPPDISLYHLVQSLVPFDPSKLFGWQSTNTLAVSDASSDLPHFSSPELVNKYAVMERLDFLYYLRHGRPSFAFGTFLVQQLVKSKSPKQLIQQAGNEAYLLGLSFFNTPSVAAACVCFLELLGLSSLKLRVDIKVANMIFIYKTRNEETQHNQIRESLVEQLMRLADGEKAATEEVLKSLEEAFWDSIEHQGIKRTSSDSRQQWSLVMQFCKLHAMKLSTSYLKECAKSNDWLQFIIQAQLYSYKPDEVSSVLEGFAPIIQRHLMLALEKSPFLHAAAGAPEGSTGSRCADSLFQILFRCQESPNASCHLLAEGLREHAPILSVLAACSPDADIIHCLCVWIITSVDDVTRSEATKHIQSSVENHKWDLHDLSLIWKVLLRKQKCQTLIRGFQLFLKDSPLLHILEMYDLCMNCKRYYEAKTKLDEFQKHLTNLKTTDGAAASGVPVQWLESQALFLLELMMQQCRTEYELGKLLQLFAATDNLLLDGPYVKKLCALSETLRDSSVPINRSMLTRYTPETFQKECTSILEQLQEKGMFSLAREVAELAELPADSVVTQEILRDIRLLRDTGEWDRDQIRIKFWKKCNDSFVKNSISSKAASDFFLNQGNVVSESPVREKINSISERHLLLTLAGHWLAKDVSVALDKLEEIEKQIWICRITQQMLSTDEGPGKSGRSSCAAANGDFTFDNLAKEFSFSKLPALNTPNYLNVEGLELTGCQQTLAAEEEESLRFLVGCLLDEGSVHEASRVCRYFHFYSRDVSLVLHCRALAAGEADLDRLHADIQALVAIEKTQDSSASQQSRVPSTSSLEDWTHIADPSPDDQIVVSLKALTDECVHGRNYCRQVLCLYELSKELNCSYSEMSALDPEKVLRAILSSQQAERCRRAQAFITTQGLQPETVAELVAKEVVQELLASSEGKGQKQVSNPAAESQAFLQLAKLCQDHTLVGMKLLEKISSVPRGELSCITELLILAHNCFSLTCHMEGITRVLQAARLLTDEHLAPNDEYGLMVRLLTGIGRYNEMTYIFELLHEKHYFEVLMRKKLDPSGTLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAACIQLKLIESQPWEESLQDVANLKKLLMKALTLFIDAAESYSKDSCVRQALRCRRLTKLITLQLHFLSTGQSTMLINRNRQNLMECIVSLPRFYQAAVVAEAYEFVPDWAEVLYQQVIAKGDFSYLEEFKQQRLLKPAMFEEIAKKCKQHPPSDAALRNLKKLLAYCEDISTYYRLAYDNKFYDVVNMLLKDAQTGCCLNDMLSS

Target ORF information:

RefSeq Version	XM_003641831.4
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003641831.4

ORF Insert Sequence:

ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC 
CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG 
CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT 
GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC 
TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG 
GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG 
AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG 
CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT 
TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT 
TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA 
CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC 
ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT 
TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT 
CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG 
GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC 
AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT 
TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA 
GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT 
TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG 
CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT 
AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA 
CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC 
GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC 
TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC 
AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA 
GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA 
AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA 
GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG 
CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG 
CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA 
GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA 
CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT 
GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT 
GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC 
CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG 
GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG 
GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA 
CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA 
ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA 
GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT 
TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC 
AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC 
CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG 
ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC 
AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG 
TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT 
GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA 
GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT 
GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA 
GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA 
GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT 
CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA 
GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG 
TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG 
AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT 
CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT 
GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC 
CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC 
AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG 
TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG 
ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA 
GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA 
GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG 
CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA 
GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT 
CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC 
TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT 
GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA 
TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG 
TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA 
GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT 
TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT 
GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC 
AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT 
GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG 
GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG 
TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC 
CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG 
GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA 
TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT 
GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC 
TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG 
CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG 
CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA 
GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC 
GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG 
GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT 
ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA 
ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG 
CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC 
TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC 
GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG 
CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT 
TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG 
GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA 
GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA 
GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG 
GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGTTCT GTGTCTGTAT 
GAACTTTCTA AGGAACTGAA CTGCTCCTAC AGTGAGATGT CAGCCCTTGA CCCTGAGAAA 
GTGCTTCGAG CAATTCTGTC ATCCCAGCAG GCAGAGCGCT GCAGGAGGGC TCAGGCGTTC 
ATCACCACGC AGGGCCTGCA GCCCGAAACT GTGGCAGAGC TGGTGGCCAA AGAGGTCGTG 
CAGGAGTTAC TGGCCTCTTC AGAAGGAAAA GGACAGAAGC AGGTTTCCAA CCCTGCAGCT 
GAGAGCCAGG CGTTCCTGCA GCTCGCAAAG CTGTGCCAGG ACCACACGCT GGTGGGCATG 
AAGCTGCTGG AGAAAATTTC CTCTGTGCCA CGTGGAGAAC TGTCATGCAT TACAGAACTG 
CTGATTCTGG CCCATAATTG CTTTAGTCTG ACTTGCCACA TGGAAGGAAT CACCAGAGTC 
CTGCAGGCAG CACGGCTGCT CACAGATGAG CATTTGGCAC CTAATGATGA GTATGGACTT 
ATGGTTCGTC TTCTCACTGG GATCGGCAGA TACAATGAGA TGACCTACAT CTTTGAGCTG 
CTGCACGAGA AGCATTACTT TGAAGTGCTC ATGAGGAAGA AGTTGGACCC GAGTGGGACA 
CTGAAGACAG CTCTGCTGGA TTACATCAAG CGCTGCCGCC CAGGGGACAG TGAGAAGCAC 
AACATGATTG CCCTTTGCTT CAGCATGTGC CGGGAAATTG GAGAGAATCA CGAGGCTGCT 
GCTTGCATAC AGCTGAAACT CATTGAATCT CAGCCATGGG AGGAGTCCCT TCAGGATGTT 
GCAAACTTAA AGAAACTGCT GATGAAAGCT CTGACCCTCT TCATTGATGC AGCAGAAAGT 
TACTCTAAGG ACTCCTGTGT CCGCCAGGCG CTGCGCTGCC GCCGCCTCAC AAAGCTGATC 
ACTCTGCAGC TGCATTTTCT GAGCACCGGT CAGAGCACGA TGCTGATTAA CAGGAACAGG 
CAGAACCTCA TGGAATGCAT CGTGTCCCTG CCTCGATTCT ATCAGGCGGC GGTTGTGGCC 
GAGGCATATG AGTTTGTTCC AGATTGGGCT GAAGTTCTGT ACCAACAAGT GATCGCTAAG 
GGAGACTTCA GCTACTTAGA AGAATTTAAA CAGCAGAGAC TGCTGAAGCC TGCCATGTTT 
GAGGAGATTG CGAAGAAGTG TAAGCAGCAC CCACCCAGCG ATGCTGCTCT GAGGAATCTG 
AAGAAGCTGC TCGCTTACTG CGAGGACATC TCCACGTACT ACAGGCTGGC ATATGATAAC 
AAGTTCTATG ATGTGGTCAA TATGCTCCTG AAGGACGCGC AGACTGGATG CTGCCTTAAT 
GATATGTTAT CCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_004943936.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004943993.1 Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X2, mRNA.

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CloneID	OGa13354
Clone ID Related Accession (Same CDS sequence)	XM_004943936.3 , XM_004943936.2
Accession Version	XM_004943936.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	spatacsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004943936.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC 
CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG 
CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT 
GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC 
TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG 
GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG 
AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG 
CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT 
TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT 
TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA 
CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC 
ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT 
TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT 
CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG 
GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC 
AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT 
TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA 
GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT 
TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG 
CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT 
AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA 
CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC 
GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC 
TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC 
AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA 
GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA 
AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA 
GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG 
CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG 
CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA 
GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA 
CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT 
GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT 
GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC 
CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG 
GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG 
GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA 
CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA 
ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA 
GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT 
TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC 
AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC 
CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG 
ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC 
AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG 
TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT 
GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA 
GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT 
GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA 
GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA 
GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT 
CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA 
GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG 
TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG 
AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT 
CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT 
GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC 
CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC 
AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG 
TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG 
ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA 
GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA 
GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG 
CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA 
GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT 
CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC 
TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT 
GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA 
TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG 
TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA 
GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT 
TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT 
GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC 
AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT 
GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG 
GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG 
TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC 
CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG 
GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA 
TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT 
GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC 
TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG 
CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG 
CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA 
GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC 
GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG 
GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT 
ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA 
ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG 
CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC 
TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC 
GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG 
CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT 
TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG 
GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA 
GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA 
GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG 
GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGTTCT GTGTCTGTAT 
GAACTTTCTA AGGAACTGAA CTGCTCCTAC AGTGAGATGT CAGCCCTTGA CCCTGAGAAA 
GTGCTTCGAG CAATTCTGTC ATCCCAGCAG GCAGAGCGCT GCAGGAGGGC TCAGGCGTTC 
ATCACCACGC AGGGCCTGCA GCCCGAAACT GTGGCAGAGC TGGTGGCCAA AGAGGTCGTG 
CAGGAGTTAC TGGCCTCTTC AGAAGGAAAA GGACAGAAGC AGGTTTCCAA CCCTGCAGCT 
GAGAGCCAGG CGTTCCTGCA GCTCGCAAAG CTGTGCCAGG ACCACACGCT GGTGGGCATG 
AAGCTGCTGG AGAAAATTTC CTCTGTGCCA CGTGGAGAAC TGTCATGCAT TACAGAACTG 
CTGATTCTGG CCCATAATTG CTTTAGTCTG ACTTGCCACA TGGAAGGAAT CACCAGAGTC 
CTGCAGGCAG CACGGCTGCT CACAGATGAG CATTTGGCAC CTAATGATGA GTATGGACTT 
ATGGTTCGTC TTCTCACTGG GATCGGCAGA TACAATGAGA TGACCTACAT CTTTGAGCTG 
CTGCACGAGA AGCATTACTT TGAAGTGCTC ATGAGGAAGA AGTTGGACCC GAGTGGGACA 
CTGAAGACAG CTCTGCTGGA TTACATCAAG CGCTGCCGCC CAGGGGACAG TGAGAAGCAC 
AACATGATTG CCCTTTGCTT CAGCATGTGC CGGGAAATTG GAGAGAATCA CGAGGCTGCT 
GCTTGCATAC AGCTGAAACT CATTGAATCT CAGCCATGGG AGGAGTCCCT TCAGGATGTT 
GCAAACTTAA AGAAACTGCT GATGAAAGCT CTGACCCTCT TCATTGATGC AGCAGAAAGT 
TACTCTAAGG CGCTGCGCTG CCGCCGCCTC ACAAAGCTGA TCACTCTGCA GCTGCATTTT 
CTGAGCACCG GTCAGAGCAC GATGCTGATT AACAGGAACA GGCAGAACCT CATGGAATGC 
ATCGTGTCCC TGCCTCGATT CTATCAGGCG GCGGTTGTGG CCGAGGCATA TGAGTTTGTT 
CCAGATTGGG CTGAAGTTCT GTACCAACAA GTGATCGCTA AGGGAGACTT CAGCTACTTA 
GAAGAATTTA AACAGCAGAG ACTGCTGAAG CCTGCCATGT TTGAGGAGAT TGCGAAGAAG 
TGTAAGCAGC ACCCACCCAG CGATGCTGCT CTGAGGAATC TGAAGAAGCT GCTCGCTTAC 
TGCGAGGACA TCTCCACGTA CTACAGGCTG GCATATGATA ACAAGTTCTA TGATGTGGTC 
AATATGCTCC TGAAGGACGC GCAGACTGGA TGCTGCCTTA ATGATATGTT ATCCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004943993.1
CDS	143..7402
Translation	MAGARRELRVLLAARPGQARDSVRDAARVRLSAEQDALGSLLLRDGIYLESLGPGGGGRARSGVLPGAWADFVWERTAEYDPQAKRTKILALSESHDLFVYEFTREDGKCGPDPLHSCREGVLKKLLEANSVSLSSILSLRILSFKDNKCTLLLNSFIVMHLTFPGEHSSLETCDCFTLDLPPPVLERVTDINFCRGILFFLDSSGWIYVFDSSDGANLAHINVSLCQGGVQDGDRSPAITSPLTALRVSYDLSVAVIISSSSNACSVDLNVYFRQFPGHLICRRDSESLPLKPPEGVDEDDLASSDYSMELLSSPFRTDRSWKAHLSSLCSTIRKRRSRSPHLLCDMNLPWYQYFTHLEDHDPLIYETSEKALAIAPKAFPRVLSNPSQSAGTKLSDMGQQWKQILPVQLSQEVVKLECKSVTGAKALFVILTDDKALTLALWDFESQDVAHYQFGKNSVYVDCNEEVPLCLLLTECGLSLILFGLTQEEFLNRLMIYGSAGVVDSVCHLNGWGRCSIPVHALEAGLENRQLDTVDLFLKSKESLFNLSALRSEAQQSADGASCSFLKNLQELRPALNLLCSAIRENDMEPQSKHFSEQLLNLTLTFLSKQLEEIFVHTEEPDRYLQKAAEILTDYIIELRTFMKNYPRSQVNTTNVGSDSDEDLPAIEESQMWEKLTPEEVIAEAILSNKMPEAQIFFRMHRHPAQSLQELIRTGLDLVYGHLLKGNTREASALLKNMGFDVKQQLHKICFYTADKHVRDLLVKVLQEENYFSEKEKKMIDFVHQVESLYSESFQENKEMQSLSSVRNWRQEQDSSKHLATLESLMDHDGRRVPSREVRVMLNWAQCWDELLQDMILLPRKQSQGLATCNPEALWMYLTAQHDWLNISSWIEEFEPNHSLCGQSKWPPLAASIVDQHTLCSGYMRNEILNKLARNGIFVLSELEDFELLLQRLVSLGGVLQNPHPVPKYSAASGLDFHARFVLHCLEHNLQYMLYTYLEYYSLTPSNCPILDDKELHEAHPWFEFLVQCRGVASNPRDPKMIFQASLANAQILIPSNQASVSSMLLEGRTLLALATTMYAPGGIDQVLQNEDTEKPLKKVDPQLLKMALSPYPKLRAALFPPCTSHGILPPDISLYHLVQSLVPFDPSKLFGWQSTNTLAVSDASSDLPHFSSPELVNKYAVMERLDFLYYLRHGRPSFAFGTFLVQQLVKSKSPKQLIQQAGNEAYLLGLSFFNTPSVAAACVCFLELLGLSSLKLRVDIKVANMIFIYKTRNEETQHNQIRESLVEQLMRLADGEKAATEEVLKSLEEAFWDSIEHQGIKRTSSDSRQQWSLVMQFCKLHAMKLSTSYLKECAKSNDWLQFIIQAQLYSYKPDEVSSVLEGFAPIIQRHLMLALEKSPFLHAAAGAPEGSTGSRCADSLFQILFRCQESPNASCHLLAEGLREHAPILSVLAACSPDADIIHCLCVWIITSVDDVTRSEATKHIQSSVENHKWDLHDLSLIWKVLLRKQKCQTLIRGFQLFLKDSPLLHILEMYDLCMNCKRYYEAKTKLDEFQKHLTNLKTTDGAAASGVPVQWLESQALFLLELMMQQCRTEYELGKLLQLFAATDNLLLDGPYVKKLCALSETLRDSSVPINRSMLTRYTPETFQKECTSILEQLQEKGMFSLAREVAELAELPADSVVTQEILRDIRLLRDTGEWDRDQIRIKFWKKCNDSFVKNSISSKAASDFFLNQGNVVSESPVREKINSISERHLLLTLAGHWLAKDVSVALDKLEEIEKQIWICRITQQMLSTDEGPGKSGRSSCAAANGDFTFDNLAKEFSFSKLPALNTPNYLNVEGLELTGCQQTLAAEEEESLRFLVGCLLDEGSVHEASRVCRYFHFYSRDVSLVLHCRALAAGEADLDRLHADIQALVAIEKTQDSSASQQSRVPSTSSLEDWTHIADPSPDDQIVVSLKALTDECVHGRNYCRQVLCLYELSKELNCSYSEMSALDPEKVLRAILSSQQAERCRRAQAFITTQGLQPETVAELVAKEVVQELLASSEGKGQKQVSNPAAESQAFLQLAKLCQDHTLVGMKLLEKISSVPRGELSCITELLILAHNCFSLTCHMEGITRVLQAARLLTDEHLAPNDEYGLMVRLLTGIGRYNEMTYIFELLHEKHYFEVLMRKKLDPSGTLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAACIQLKLIESQPWEESLQDVANLKKLLMKALTLFIDAAESYSKALRCRRLTKLITLQLHFLSTGQSTMLINRNRQNLMECIVSLPRFYQAAVVAEAYEFVPDWAEVLYQQVIAKGDFSYLEEFKQQRLLKPAMFEEIAKKCKQHPPSDAALRNLKKLLAYCEDISTYYRLAYDNKFYDVVNMLLKDAQTGCCLNDMLSS

Target ORF information:

RefSeq Version	XM_004943936.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004943936.3

ORF Insert Sequence:

ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC 
CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG 
CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT 
GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC 
TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG 
GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG 
AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG 
CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT 
TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT 
TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA 
CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC 
ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT 
TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT 
CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG 
GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC 
AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT 
TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA 
GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT 
TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG 
CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT 
AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA 
CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC 
GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC 
TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC 
AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA 
GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA 
AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA 
GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG 
CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG 
CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA 
GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA 
CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT 
GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT 
GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC 
CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG 
GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG 
GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA 
CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA 
ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA 
GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT 
TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC 
AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC 
CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG 
ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC 
AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG 
TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT 
GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA 
GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT 
GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA 
GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA 
GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT 
CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA 
GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG 
TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG 
AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT 
CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT 
GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC 
CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC 
AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG 
TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG 
ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA 
GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA 
GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG 
CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA 
GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT 
CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC 
TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT 
GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA 
TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG 
TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA 
GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT 
TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT 
GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC 
AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT 
GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG 
GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG 
TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC 
CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG 
GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA 
TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT 
GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC 
TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG 
CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG 
CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA 
GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC 
GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG 
GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT 
ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA 
ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG 
CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC 
TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC 
GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG 
CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT 
TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG 
GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA 
GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA 
GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG 
GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGTTCT GTGTCTGTAT 
GAACTTTCTA AGGAACTGAA CTGCTCCTAC AGTGAGATGT CAGCCCTTGA CCCTGAGAAA 
GTGCTTCGAG CAATTCTGTC ATCCCAGCAG GCAGAGCGCT GCAGGAGGGC TCAGGCGTTC 
ATCACCACGC AGGGCCTGCA GCCCGAAACT GTGGCAGAGC TGGTGGCCAA AGAGGTCGTG 
CAGGAGTTAC TGGCCTCTTC AGAAGGAAAA GGACAGAAGC AGGTTTCCAA CCCTGCAGCT 
GAGAGCCAGG CGTTCCTGCA GCTCGCAAAG CTGTGCCAGG ACCACACGCT GGTGGGCATG 
AAGCTGCTGG AGAAAATTTC CTCTGTGCCA CGTGGAGAAC TGTCATGCAT TACAGAACTG 
CTGATTCTGG CCCATAATTG CTTTAGTCTG ACTTGCCACA TGGAAGGAAT CACCAGAGTC 
CTGCAGGCAG CACGGCTGCT CACAGATGAG CATTTGGCAC CTAATGATGA GTATGGACTT 
ATGGTTCGTC TTCTCACTGG GATCGGCAGA TACAATGAGA TGACCTACAT CTTTGAGCTG 
CTGCACGAGA AGCATTACTT TGAAGTGCTC ATGAGGAAGA AGTTGGACCC GAGTGGGACA 
CTGAAGACAG CTCTGCTGGA TTACATCAAG CGCTGCCGCC CAGGGGACAG TGAGAAGCAC 
AACATGATTG CCCTTTGCTT CAGCATGTGC CGGGAAATTG GAGAGAATCA CGAGGCTGCT 
GCTTGCATAC AGCTGAAACT CATTGAATCT CAGCCATGGG AGGAGTCCCT TCAGGATGTT 
GCAAACTTAA AGAAACTGCT GATGAAAGCT CTGACCCTCT TCATTGATGC AGCAGAAAGT 
TACTCTAAGG CGCTGCGCTG CCGCCGCCTC ACAAAGCTGA TCACTCTGCA GCTGCATTTT 
CTGAGCACCG GTCAGAGCAC GATGCTGATT AACAGGAACA GGCAGAACCT CATGGAATGC 
ATCGTGTCCC TGCCTCGATT CTATCAGGCG GCGGTTGTGG CCGAGGCATA TGAGTTTGTT 
CCAGATTGGG CTGAAGTTCT GTACCAACAA GTGATCGCTA AGGGAGACTT CAGCTACTTA 
GAAGAATTTA AACAGCAGAG ACTGCTGAAG CCTGCCATGT TTGAGGAGAT TGCGAAGAAG 
TGTAAGCAGC ACCCACCCAG CGATGCTGCT CTGAGGAATC TGAAGAAGCT GCTCGCTTAC 
TGCGAGGACA TCTCCACGTA CTACAGGCTG GCATATGATA ACAAGTTCTA TGATGTGGTC 
AATATGCTCC TGAAGGACGC GCAGACTGGA TGCTGCCTTA ATGATATGTT ATCCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_004943936.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004943993.1 Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X2, mRNA.

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CloneID	OGa13354
Clone ID Related Accession (Same CDS sequence)	XM_004943936.3 , XM_004943936.2
Accession Version	XM_004943936.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	spatacsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455925.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004943936.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC 
CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG 
CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT 
GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC 
TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG 
GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG 
AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG 
CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT 
TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT 
TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA 
CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC 
ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT 
TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT 
CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG 
GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC 
AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT 
TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA 
GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT 
TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG 
CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT 
AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA 
CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC 
GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC 
TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC 
AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA 
GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA 
AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA 
GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG 
CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG 
CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA 
GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA 
CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT 
GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT 
GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC 
CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG 
GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG 
GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA 
CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA 
ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA 
GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT 
TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC 
AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC 
CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG 
ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC 
AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG 
TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT 
GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA 
GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT 
GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA 
GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA 
GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT 
CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA 
GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG 
TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG 
AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT 
CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT 
GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC 
CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC 
AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG 
TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG 
ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA 
GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA 
GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG 
CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA 
GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT 
CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC 
TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT 
GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA 
TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG 
TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA 
GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT 
TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT 
GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC 
AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT 
GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG 
GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG 
TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC 
CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG 
GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA 
TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT 
GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC 
TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG 
CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG 
CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA 
GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC 
GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG 
GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT 
ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA 
ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG 
CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC 
TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC 
GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG 
CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT 
TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG 
GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA 
GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA 
GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG 
GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGTTCT GTGTCTGTAT 
GAACTTTCTA AGGAACTGAA CTGCTCCTAC AGTGAGATGT CAGCCCTTGA CCCTGAGAAA 
GTGCTTCGAG CAATTCTGTC ATCCCAGCAG GCAGAGCGCT GCAGGAGGGC TCAGGCGTTC 
ATCACCACGC AGGGCCTGCA GCCCGAAACT GTGGCAGAGC TGGTGGCCAA AGAGGTCGTG 
CAGGAGTTAC TGGCCTCTTC AGAAGGAAAA GGACAGAAGC AGGTTTCCAA CCCTGCAGCT 
GAGAGCCAGG CGTTCCTGCA GCTCGCAAAG CTGTGCCAGG ACCACACGCT GGTGGGCATG 
AAGCTGCTGG AGAAAATTTC CTCTGTGCCA CGTGGAGAAC TGTCATGCAT TACAGAACTG 
CTGATTCTGG CCCATAATTG CTTTAGTCTG ACTTGCCACA TGGAAGGAAT CACCAGAGTC 
CTGCAGGCAG CACGGCTGCT CACAGATGAG CATTTGGCAC CTAATGATGA GTATGGACTT 
ATGGTTCGTC TTCTCACTGG GATCGGCAGA TACAATGAGA TGACCTACAT CTTTGAGCTG 
CTGCACGAGA AGCATTACTT TGAAGTGCTC ATGAGGAAGA AGTTGGACCC GAGTGGGACA 
CTGAAGACAG CTCTGCTGGA TTACATCAAG CGCTGCCGCC CAGGGGACAG TGAGAAGCAC 
AACATGATTG CCCTTTGCTT CAGCATGTGC CGGGAAATTG GAGAGAATCA CGAGGCTGCT 
GCTTGCATAC AGCTGAAACT CATTGAATCT CAGCCATGGG AGGAGTCCCT TCAGGATGTT 
GCAAACTTAA AGAAACTGCT GATGAAAGCT CTGACCCTCT TCATTGATGC AGCAGAAAGT 
TACTCTAAGG CGCTGCGCTG CCGCCGCCTC ACAAAGCTGA TCACTCTGCA GCTGCATTTT 
CTGAGCACCG GTCAGAGCAC GATGCTGATT AACAGGAACA GGCAGAACCT CATGGAATGC 
ATCGTGTCCC TGCCTCGATT CTATCAGGCG GCGGTTGTGG CCGAGGCATA TGAGTTTGTT 
CCAGATTGGG CTGAAGTTCT GTACCAACAA GTGATCGCTA AGGGAGACTT CAGCTACTTA 
GAAGAATTTA AACAGCAGAG ACTGCTGAAG CCTGCCATGT TTGAGGAGAT TGCGAAGAAG 
TGTAAGCAGC ACCCACCCAG CGATGCTGCT CTGAGGAATC TGAAGAAGCT GCTCGCTTAC 
TGCGAGGACA TCTCCACGTA CTACAGGCTG GCATATGATA ACAAGTTCTA TGATGTGGTC 
AATATGCTCC TGAAGGACGC GCAGACTGGA TGCTGCCTTA ATGATATGTT ATCCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004943993.1
CDS	104..7363
Translation	MAGARRELRVLLAARPGQARDSVRDAARVRLSAEQDALGSLLLRDGIYLESLGPGGGGRARSGVLPGAWADFVWERTAEYDPQAKRTKILALSESHDLFVYEFTREDGKCGPDPLHSCREGVLKKLLEANSVSLSSILSLRILSFKDNKCTLLLNSFIVMHLTFPGEHSSLETCDCFTLDLPPPVLERVTDINFCRGILFFLDSSGWIYVFDSSDGANLAHINVSLCQGGVQDGDRSPAITSPLTALRVSYDLSVAVIISSSSNACSVDLNVYFRQFPGHLICRRDSESLPLKPPEGVDEDDLASSDYSMELLSSPFRTDRSWKAHLSSLCSTIRKRRSRSPHLLCDMNLPWYQYFTHLEDHDPLIYETSEKALAIAPKAFPRVLSNPSQSAGTKLSDMGQQWKQILPVQLSQEVVKLECKSVTGAKALFVILTDDKALTLALWDFESQDVAHYQFGKNSVYVDCNEEVPLCLLLTECGLSLILFGLTQEEFLNRLMIYGSAGVVDSVCHLNGWGRCSIPVHALEAGLENRQLDTVDLFLKSKESLFNLSALRSEAQQSADGASCSFLKNLQELRPALNLLCSAIRENDMEPQSKHFSEQLLNLTLTFLSKQLEEIFVHTEEPDRYLQKAAEILTDYIIELRTFMKNYPRSQVNTTNVGSDSDEDLPAIEESQMWEKLTPEEVIAEAILSNKMPEAQIFFRMHRHPAQSLQELIRTGLDLVYGHLLKGNTREASALLKNMGFDVKQQLHKICFYTADKHVRDLLVKVLQEENYFSEKEKKMIDFVHQVESLYSESFQENKEMQSLSSVRNWRQEQDSSKHLATLESLMDHDGRRVPSREVRVMLNWAQCWDELLQDMILLPRKQSQGLATCNPEALWMYLTAQHDWLNISSWIEEFEPNHSLCGQSKWPPLAASIVDQHTLCSGYMRNEILNKLARNGIFVLSELEDFELLLQRLVSLGGVLQNPHPVPKYSAASGLDFHARFVLHCLEHNLQYMLYTYLEYYSLTPSNCPILDDKELHEAHPWFEFLVQCRGVASNPRDPKMIFQASLANAQILIPSNQASVSSMLLEGRTLLALATTMYAPGGIDQVLQNEDTEKPLKKVDPQLLKMALSPYPKLRAALFPPCTSHGILPPDISLYHLVQSLVPFDPSKLFGWQSTNTLAVSDASSDLPHFSSPELVNKYAVMERLDFLYYLRHGRPSFAFGTFLVQQLVKSKSPKQLIQQAGNEAYLLGLSFFNTPSVAAACVCFLELLGLSSLKLRVDIKVANMIFIYKTRNEETQHNQIRESLVEQLMRLADGEKAATEEVLKSLEEAFWDSIEHQGIKRTSSDSRQQWSLVMQFCKLHAMKLSTSYLKECAKSNDWLQFIIQAQLYSYKPDEVSSVLEGFAPIIQRHLMLALEKSPFLHAAAGAPEGSTGSRCADSLFQILFRCQESPNASCHLLAEGLREHAPILSVLAACSPDADIIHCLCVWIITSVDDVTRSEATKHIQSSVENHKWDLHDLSLIWKVLLRKQKCQTLIRGFQLFLKDSPLLHILEMYDLCMNCKRYYEAKTKLDEFQKHLTNLKTTDGAAASGVPVQWLESQALFLLELMMQQCRTEYELGKLLQLFAATDNLLLDGPYVKKLCALSETLRDSSVPINRSMLTRYTPETFQKECTSILEQLQEKGMFSLAREVAELAELPADSVVTQEILRDIRLLRDTGEWDRDQIRIKFWKKCNDSFVKNSISSKAASDFFLNQGNVVSESPVREKINSISERHLLLTLAGHWLAKDVSVALDKLEEIEKQIWICRITQQMLSTDEGPGKSGRSSCAAANGDFTFDNLAKEFSFSKLPALNTPNYLNVEGLELTGCQQTLAAEEEESLRFLVGCLLDEGSVHEASRVCRYFHFYSRDVSLVLHCRALAAGEADLDRLHADIQALVAIEKTQDSSASQQSRVPSTSSLEDWTHIADPSPDDQIVVSLKALTDECVHGRNYCRQVLCLYELSKELNCSYSEMSALDPEKVLRAILSSQQAERCRRAQAFITTQGLQPETVAELVAKEVVQELLASSEGKGQKQVSNPAAESQAFLQLAKLCQDHTLVGMKLLEKISSVPRGELSCITELLILAHNCFSLTCHMEGITRVLQAARLLTDEHLAPNDEYGLMVRLLTGIGRYNEMTYIFELLHEKHYFEVLMRKKLDPSGTLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAACIQLKLIESQPWEESLQDVANLKKLLMKALTLFIDAAESYSKALRCRRLTKLITLQLHFLSTGQSTMLINRNRQNLMECIVSLPRFYQAAVVAEAYEFVPDWAEVLYQQVIAKGDFSYLEEFKQQRLLKPAMFEEIAKKCKQHPPSDAALRNLKKLLAYCEDISTYYRLAYDNKFYDVVNMLLKDAQTGCCLNDMLSS

Target ORF information:

RefSeq Version	XM_004943936.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004943936.2

ORF Insert Sequence:

ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC 
CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG 
CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT 
GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC 
TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG 
GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG 
AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG 
CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT 
TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT 
TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA 
CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC 
ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT 
TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT 
CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG 
GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC 
AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT 
TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA 
GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT 
TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG 
CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT 
AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA 
CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC 
GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC 
TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC 
AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA 
GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA 
AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA 
GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG 
CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG 
CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA 
GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA 
CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT 
GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT 
GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC 
CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG 
GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG 
GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA 
CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA 
ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA 
GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT 
TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC 
AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC 
CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG 
ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC 
AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG 
TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT 
GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA 
GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT 
GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA 
GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA 
GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT 
CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA 
GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG 
TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG 
AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT 
CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT 
GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC 
CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC 
AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG 
TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG 
ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA 
GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA 
GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG 
CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA 
GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT 
CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC 
TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT 
GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA 
TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG 
TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA 
GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT 
TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT 
GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC 
AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT 
GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG 
GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG 
TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC 
CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG 
GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA 
TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT 
GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC 
TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG 
CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG 
CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA 
GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC 
GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG 
GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT 
ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA 
ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG 
CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC 
TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC 
GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG 
CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT 
TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG 
GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA 
GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA 
GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG 
GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGTTCT GTGTCTGTAT 
GAACTTTCTA AGGAACTGAA CTGCTCCTAC AGTGAGATGT CAGCCCTTGA CCCTGAGAAA 
GTGCTTCGAG CAATTCTGTC ATCCCAGCAG GCAGAGCGCT GCAGGAGGGC TCAGGCGTTC 
ATCACCACGC AGGGCCTGCA GCCCGAAACT GTGGCAGAGC TGGTGGCCAA AGAGGTCGTG 
CAGGAGTTAC TGGCCTCTTC AGAAGGAAAA GGACAGAAGC AGGTTTCCAA CCCTGCAGCT 
GAGAGCCAGG CGTTCCTGCA GCTCGCAAAG CTGTGCCAGG ACCACACGCT GGTGGGCATG 
AAGCTGCTGG AGAAAATTTC CTCTGTGCCA CGTGGAGAAC TGTCATGCAT TACAGAACTG 
CTGATTCTGG CCCATAATTG CTTTAGTCTG ACTTGCCACA TGGAAGGAAT CACCAGAGTC 
CTGCAGGCAG CACGGCTGCT CACAGATGAG CATTTGGCAC CTAATGATGA GTATGGACTT 
ATGGTTCGTC TTCTCACTGG GATCGGCAGA TACAATGAGA TGACCTACAT CTTTGAGCTG 
CTGCACGAGA AGCATTACTT TGAAGTGCTC ATGAGGAAGA AGTTGGACCC GAGTGGGACA 
CTGAAGACAG CTCTGCTGGA TTACATCAAG CGCTGCCGCC CAGGGGACAG TGAGAAGCAC 
AACATGATTG CCCTTTGCTT CAGCATGTGC CGGGAAATTG GAGAGAATCA CGAGGCTGCT 
GCTTGCATAC AGCTGAAACT CATTGAATCT CAGCCATGGG AGGAGTCCCT TCAGGATGTT 
GCAAACTTAA AGAAACTGCT GATGAAAGCT CTGACCCTCT TCATTGATGC AGCAGAAAGT 
TACTCTAAGG CGCTGCGCTG CCGCCGCCTC ACAAAGCTGA TCACTCTGCA GCTGCATTTT 
CTGAGCACCG GTCAGAGCAC GATGCTGATT AACAGGAACA GGCAGAACCT CATGGAATGC 
ATCGTGTCCC TGCCTCGATT CTATCAGGCG GCGGTTGTGG CCGAGGCATA TGAGTTTGTT 
CCAGATTGGG CTGAAGTTCT GTACCAACAA GTGATCGCTA AGGGAGACTT CAGCTACTTA 
GAAGAATTTA AACAGCAGAG ACTGCTGAAG CCTGCCATGT TTGAGGAGAT TGCGAAGAAG 
TGTAAGCAGC ACCCACCCAG CGATGCTGCT CTGAGGAATC TGAAGAAGCT GCTCGCTTAC 
TGCGAGGACA TCTCCACGTA CTACAGGCTG GCATATGATA ACAAGTTCTA TGATGTGGTC 
AATATGCTCC TGAAGGACGC GCAGACTGGA TGCTGCCTTA ATGATATGTT ATCCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_004943937.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004943994.1 Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X3, mRNA.

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CloneID	OGa13355
Clone ID Related Accession (Same CDS sequence)	XM_004943937.2 , XM_004943937.3
Accession Version	XM_004943937.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	spatacsin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455925.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004943937.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC 
CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG 
CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT 
GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC 
TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG 
GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG 
AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG 
CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT 
TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT 
TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA 
CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC 
ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT 
TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT 
CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG 
GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC 
AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT 
TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA 
GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT 
TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG 
CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT 
AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA 
CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC 
GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC 
TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC 
AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA 
GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA 
AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA 
GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG 
CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG 
CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA 
GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA 
CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT 
GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT 
GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC 
CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG 
GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG 
GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA 
CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA 
ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA 
GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT 
TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC 
AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC 
CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG 
ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC 
AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG 
TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT 
GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA 
GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT 
GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA 
GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA 
GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT 
CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA 
GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG 
TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG 
AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT 
CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT 
GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC 
CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC 
AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG 
TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG 
ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA 
GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA 
GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG 
CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA 
GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT 
CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC 
TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT 
GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA 
TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG 
TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA 
GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT 
TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT 
GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC 
AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT 
GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG 
GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG 
TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC 
CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG 
GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA 
TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT 
GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC 
TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG 
CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG 
CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA 
GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC 
GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG 
GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT 
ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA 
ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG 
CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC 
TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC 
GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG 
CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT 
TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG 
GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA 
GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA 
GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG 
GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGAACT GAACTGCTCC 
TACAGTGAGA TGTCAGCCCT TGACCCTGAG AAAGTGCTTC GAGCAATTCT GTCATCCCAG 
CAGGCAGAGC GCTGCAGGAG GGCTCAGGCG TTCATCACCA CGCAGGGCCT GCAGCCCGAA 
ACTGTGGCAG AGCTGGTGGC CAAAGAGGTC GTGCAGGAGT TACTGGCCTC TTCAGAAGGA 
AAAGGACAGA AGCAGGTTTC CAACCCTGCA GCTGAGAGCC AGGCGTTCCT GCAGCTCGCA 
AAGCTGTGCC AGGACCACAC GCTGGTGGGC ATGAAGCTGC TGGAGAAAAT TTCCTCTGTG 
CCACGTGGAG AACTGTCATG CATTACAGAA CTGCTGATTC TGGCCCATAA TTGCTTTAGT 
CTGACTTGCC ACATGGAAGG AATCACCAGA GTCCTGCAGG CAGCACGGCT GCTCACAGAT 
GAGCATTTGG CACCTAATGA TGAGTATGGA CTTATGGTTC GTCTTCTCAC TGGGATCGGC 
AGATACAATG AGATGACCTA CATCTTTGAG CTGCTGCACG AGAAGCATTA CTTTGAAGTG 
CTCATGAGGA AGAAGTTGGA CCCGAGTGGG ACACTGAAGA CAGCTCTGCT GGATTACATC 
AAGCGCTGCC GCCCAGGGGA CAGTGAGAAG CACAACATGA TTGCCCTTTG CTTCAGCATG 
TGCCGGGAAA TTGGAGAGAA TCACGAGGCT GCTGCTTGCA TACAGCTGAA ACTCATTGAA 
TCTCAGCCAT GGGAGGAGTC CCTTCAGGAT GTTGCAAACT TAAAGAAACT GCTGATGAAA 
GCTCTGACCC TCTTCATTGA TGCAGCAGAA AGTTACTCTA AGGACTCCTG TGTCCGCCAG 
GCGCTGCGCT GCCGCCGCCT CACAAAGCTG ATCACTCTGC AGCTGCATTT TCTGAGCACC 
GGTCAGAGCA CGATGCTGAT TAACAGGAAC AGGCAGAACC TCATGGAATG CATCGTGTCC 
CTGCCTCGAT TCTATCAGGC GGCGGTTGTG GCCGAGGCAT ATGAGTTTGT TCCAGATTGG 
GCTGAAGTTC TGTACCAACA AGTGATCGCT AAGGGAGACT TCAGCTACTT AGAAGAATTT 
AAACAGCAGA GACTGCTGAA GCCTGCCATG TTTGAGGAGA TTGCGAAGAA GTGTAAGCAG 
CACCCACCCA GCGATGCTGC TCTGAGGAAT CTGAAGAAGC TGCTCGCTTA CTGCGAGGAC 
ATCTCCACGT ACTACAGGCT GGCATATGAT AACAAGTTCT ATGATGTGGT CAATATGCTC 
CTGAAGGACG CGCAGACTGG ATGCTGCCTT AATGATATGT TATCCAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004943994.1
CDS	104..7354
Translation	MAGARRELRVLLAARPGQARDSVRDAARVRLSAEQDALGSLLLRDGIYLESLGPGGGGRARSGVLPGAWADFVWERTAEYDPQAKRTKILALSESHDLFVYEFTREDGKCGPDPLHSCREGVLKKLLEANSVSLSSILSLRILSFKDNKCTLLLNSFIVMHLTFPGEHSSLETCDCFTLDLPPPVLERVTDINFCRGILFFLDSSGWIYVFDSSDGANLAHINVSLCQGGVQDGDRSPAITSPLTALRVSYDLSVAVIISSSSNACSVDLNVYFRQFPGHLICRRDSESLPLKPPEGVDEDDLASSDYSMELLSSPFRTDRSWKAHLSSLCSTIRKRRSRSPHLLCDMNLPWYQYFTHLEDHDPLIYETSEKALAIAPKAFPRVLSNPSQSAGTKLSDMGQQWKQILPVQLSQEVVKLECKSVTGAKALFVILTDDKALTLALWDFESQDVAHYQFGKNSVYVDCNEEVPLCLLLTECGLSLILFGLTQEEFLNRLMIYGSAGVVDSVCHLNGWGRCSIPVHALEAGLENRQLDTVDLFLKSKESLFNLSALRSEAQQSADGASCSFLKNLQELRPALNLLCSAIRENDMEPQSKHFSEQLLNLTLTFLSKQLEEIFVHTEEPDRYLQKAAEILTDYIIELRTFMKNYPRSQVNTTNVGSDSDEDLPAIEESQMWEKLTPEEVIAEAILSNKMPEAQIFFRMHRHPAQSLQELIRTGLDLVYGHLLKGNTREASALLKNMGFDVKQQLHKICFYTADKHVRDLLVKVLQEENYFSEKEKKMIDFVHQVESLYSESFQENKEMQSLSSVRNWRQEQDSSKHLATLESLMDHDGRRVPSREVRVMLNWAQCWDELLQDMILLPRKQSQGLATCNPEALWMYLTAQHDWLNISSWIEEFEPNHSLCGQSKWPPLAASIVDQHTLCSGYMRNEILNKLARNGIFVLSELEDFELLLQRLVSLGGVLQNPHPVPKYSAASGLDFHARFVLHCLEHNLQYMLYTYLEYYSLTPSNCPILDDKELHEAHPWFEFLVQCRGVASNPRDPKMIFQASLANAQILIPSNQASVSSMLLEGRTLLALATTMYAPGGIDQVLQNEDTEKPLKKVDPQLLKMALSPYPKLRAALFPPCTSHGILPPDISLYHLVQSLVPFDPSKLFGWQSTNTLAVSDASSDLPHFSSPELVNKYAVMERLDFLYYLRHGRPSFAFGTFLVQQLVKSKSPKQLIQQAGNEAYLLGLSFFNTPSVAAACVCFLELLGLSSLKLRVDIKVANMIFIYKTRNEETQHNQIRESLVEQLMRLADGEKAATEEVLKSLEEAFWDSIEHQGIKRTSSDSRQQWSLVMQFCKLHAMKLSTSYLKECAKSNDWLQFIIQAQLYSYKPDEVSSVLEGFAPIIQRHLMLALEKSPFLHAAAGAPEGSTGSRCADSLFQILFRCQESPNASCHLLAEGLREHAPILSVLAACSPDADIIHCLCVWIITSVDDVTRSEATKHIQSSVENHKWDLHDLSLIWKVLLRKQKCQTLIRGFQLFLKDSPLLHILEMYDLCMNCKRYYEAKTKLDEFQKHLTNLKTTDGAAASGVPVQWLESQALFLLELMMQQCRTEYELGKLLQLFAATDNLLLDGPYVKKLCALSETLRDSSVPINRSMLTRYTPETFQKECTSILEQLQEKGMFSLAREVAELAELPADSVVTQEILRDIRLLRDTGEWDRDQIRIKFWKKCNDSFVKNSISSKAASDFFLNQGNVVSESPVREKINSISERHLLLTLAGHWLAKDVSVALDKLEEIEKQIWICRITQQMLSTDEGPGKSGRSSCAAANGDFTFDNLAKEFSFSKLPALNTPNYLNVEGLELTGCQQTLAAEEEESLRFLVGCLLDEGSVHEASRVCRYFHFYSRDVSLVLHCRALAAGEADLDRLHADIQALVAIEKTQDSSASQQSRVPSTSSLEDWTHIADPSPDDQIVVSLKALTDECVHGRNYCRQELNCSYSEMSALDPEKVLRAILSSQQAERCRRAQAFITTQGLQPETVAELVAKEVVQELLASSEGKGQKQVSNPAAESQAFLQLAKLCQDHTLVGMKLLEKISSVPRGELSCITELLILAHNCFSLTCHMEGITRVLQAARLLTDEHLAPNDEYGLMVRLLTGIGRYNEMTYIFELLHEKHYFEVLMRKKLDPSGTLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAACIQLKLIESQPWEESLQDVANLKKLLMKALTLFIDAAESYSKDSCVRQALRCRRLTKLITLQLHFLSTGQSTMLINRNRQNLMECIVSLPRFYQAAVVAEAYEFVPDWAEVLYQQVIAKGDFSYLEEFKQQRLLKPAMFEEIAKKCKQHPPSDAALRNLKKLLAYCEDISTYYRLAYDNKFYDVVNMLLKDAQTGCCLNDMLSS

Target ORF information:

RefSeq Version	XM_004943937.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004943937.2

ORF Insert Sequence:

ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC 
CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG 
CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT 
GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC 
TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG 
GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG 
AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG 
CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT 
TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT 
TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA 
CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC 
ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT 
TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT 
CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG 
GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC 
AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT 
TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA 
GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT 
TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG 
CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT 
AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA 
CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC 
GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC 
TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC 
AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA 
GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA 
AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA 
GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG 
CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG 
CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA 
GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA 
CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT 
GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT 
GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC 
CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG 
GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG 
GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA 
CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA 
ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA 
GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT 
TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC 
AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC 
CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG 
ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC 
AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG 
TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT 
GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA 
GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT 
GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA 
GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA 
GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT 
CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA 
GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG 
TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG 
AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT 
CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT 
GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC 
CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC 
AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG 
TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG 
ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA 
GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA 
GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG 
CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA 
GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT 
CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC 
TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT 
GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA 
TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG 
TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA 
GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT 
TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT 
GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC 
AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT 
GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG 
GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG 
TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC 
CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG 
GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA 
TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT 
GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC 
TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG 
CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG 
CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA 
GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC 
GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG 
GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT 
ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA 
ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG 
CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC 
TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC 
GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG 
CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT 
TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG 
GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA 
GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA 
GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG 
GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGAACT GAACTGCTCC 
TACAGTGAGA TGTCAGCCCT TGACCCTGAG AAAGTGCTTC GAGCAATTCT GTCATCCCAG 
CAGGCAGAGC GCTGCAGGAG GGCTCAGGCG TTCATCACCA CGCAGGGCCT GCAGCCCGAA 
ACTGTGGCAG AGCTGGTGGC CAAAGAGGTC GTGCAGGAGT TACTGGCCTC TTCAGAAGGA 
AAAGGACAGA AGCAGGTTTC CAACCCTGCA GCTGAGAGCC AGGCGTTCCT GCAGCTCGCA 
AAGCTGTGCC AGGACCACAC GCTGGTGGGC ATGAAGCTGC TGGAGAAAAT TTCCTCTGTG 
CCACGTGGAG AACTGTCATG CATTACAGAA CTGCTGATTC TGGCCCATAA TTGCTTTAGT 
CTGACTTGCC ACATGGAAGG AATCACCAGA GTCCTGCAGG CAGCACGGCT GCTCACAGAT 
GAGCATTTGG CACCTAATGA TGAGTATGGA CTTATGGTTC GTCTTCTCAC TGGGATCGGC 
AGATACAATG AGATGACCTA CATCTTTGAG CTGCTGCACG AGAAGCATTA CTTTGAAGTG 
CTCATGAGGA AGAAGTTGGA CCCGAGTGGG ACACTGAAGA CAGCTCTGCT GGATTACATC 
AAGCGCTGCC GCCCAGGGGA CAGTGAGAAG CACAACATGA TTGCCCTTTG CTTCAGCATG 
TGCCGGGAAA TTGGAGAGAA TCACGAGGCT GCTGCTTGCA TACAGCTGAA ACTCATTGAA 
TCTCAGCCAT GGGAGGAGTC CCTTCAGGAT GTTGCAAACT TAAAGAAACT GCTGATGAAA 
GCTCTGACCC TCTTCATTGA TGCAGCAGAA AGTTACTCTA AGGACTCCTG TGTCCGCCAG 
GCGCTGCGCT GCCGCCGCCT CACAAAGCTG ATCACTCTGC AGCTGCATTT TCTGAGCACC 
GGTCAGAGCA CGATGCTGAT TAACAGGAAC AGGCAGAACC TCATGGAATG CATCGTGTCC 
CTGCCTCGAT TCTATCAGGC GGCGGTTGTG GCCGAGGCAT ATGAGTTTGT TCCAGATTGG 
GCTGAAGTTC TGTACCAACA AGTGATCGCT AAGGGAGACT TCAGCTACTT AGAAGAATTT 
AAACAGCAGA GACTGCTGAA GCCTGCCATG TTTGAGGAGA TTGCGAAGAA GTGTAAGCAG 
CACCCACCCA GCGATGCTGC TCTGAGGAAT CTGAAGAAGC TGCTCGCTTA CTGCGAGGAC 
ATCTCCACGT ACTACAGGCT GGCATATGAT AACAAGTTCT ATGATGTGGT CAATATGCTC 
CTGAAGGACG CGCAGACTGG ATGCTGCCTT AATGATATGT TATCCAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_004943937.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004943994.1 Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X3, mRNA.

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CloneID	OGa13355
Clone ID Related Accession (Same CDS sequence)	XM_004943937.2 , XM_004943937.3
Accession Version	XM_004943937.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	spatacsin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004943937.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC 
CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG 
CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT 
GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC 
TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG 
GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG 
AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG 
CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT 
TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT 
TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA 
CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC 
ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT 
TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT 
CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG 
GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC 
AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT 
TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA 
GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT 
TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG 
CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT 
AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA 
CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC 
GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC 
TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC 
AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA 
GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA 
AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA 
GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG 
CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG 
CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA 
GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA 
CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT 
GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT 
GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC 
CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG 
GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG 
GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA 
CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA 
ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA 
GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT 
TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC 
AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC 
CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG 
ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC 
AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG 
TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT 
GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA 
GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT 
GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA 
GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA 
GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT 
CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA 
GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG 
TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG 
AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT 
CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT 
GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC 
CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC 
AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG 
TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG 
ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA 
GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA 
GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG 
CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA 
GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT 
CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC 
TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT 
GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA 
TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG 
TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA 
GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT 
TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT 
GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC 
AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT 
GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG 
GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG 
TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC 
CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG 
GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA 
TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT 
GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC 
TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG 
CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG 
CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA 
GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC 
GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG 
GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT 
ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA 
ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG 
CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC 
TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC 
GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG 
CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT 
TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG 
GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA 
GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA 
GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG 
GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGAACT GAACTGCTCC 
TACAGTGAGA TGTCAGCCCT TGACCCTGAG AAAGTGCTTC GAGCAATTCT GTCATCCCAG 
CAGGCAGAGC GCTGCAGGAG GGCTCAGGCG TTCATCACCA CGCAGGGCCT GCAGCCCGAA 
ACTGTGGCAG AGCTGGTGGC CAAAGAGGTC GTGCAGGAGT TACTGGCCTC TTCAGAAGGA 
AAAGGACAGA AGCAGGTTTC CAACCCTGCA GCTGAGAGCC AGGCGTTCCT GCAGCTCGCA 
AAGCTGTGCC AGGACCACAC GCTGGTGGGC ATGAAGCTGC TGGAGAAAAT TTCCTCTGTG 
CCACGTGGAG AACTGTCATG CATTACAGAA CTGCTGATTC TGGCCCATAA TTGCTTTAGT 
CTGACTTGCC ACATGGAAGG AATCACCAGA GTCCTGCAGG CAGCACGGCT GCTCACAGAT 
GAGCATTTGG CACCTAATGA TGAGTATGGA CTTATGGTTC GTCTTCTCAC TGGGATCGGC 
AGATACAATG AGATGACCTA CATCTTTGAG CTGCTGCACG AGAAGCATTA CTTTGAAGTG 
CTCATGAGGA AGAAGTTGGA CCCGAGTGGG ACACTGAAGA CAGCTCTGCT GGATTACATC 
AAGCGCTGCC GCCCAGGGGA CAGTGAGAAG CACAACATGA TTGCCCTTTG CTTCAGCATG 
TGCCGGGAAA TTGGAGAGAA TCACGAGGCT GCTGCTTGCA TACAGCTGAA ACTCATTGAA 
TCTCAGCCAT GGGAGGAGTC CCTTCAGGAT GTTGCAAACT TAAAGAAACT GCTGATGAAA 
GCTCTGACCC TCTTCATTGA TGCAGCAGAA AGTTACTCTA AGGACTCCTG TGTCCGCCAG 
GCGCTGCGCT GCCGCCGCCT CACAAAGCTG ATCACTCTGC AGCTGCATTT TCTGAGCACC 
GGTCAGAGCA CGATGCTGAT TAACAGGAAC AGGCAGAACC TCATGGAATG CATCGTGTCC 
CTGCCTCGAT TCTATCAGGC GGCGGTTGTG GCCGAGGCAT ATGAGTTTGT TCCAGATTGG 
GCTGAAGTTC TGTACCAACA AGTGATCGCT AAGGGAGACT TCAGCTACTT AGAAGAATTT 
AAACAGCAGA GACTGCTGAA GCCTGCCATG TTTGAGGAGA TTGCGAAGAA GTGTAAGCAG 
CACCCACCCA GCGATGCTGC TCTGAGGAAT CTGAAGAAGC TGCTCGCTTA CTGCGAGGAC 
ATCTCCACGT ACTACAGGCT GGCATATGAT AACAAGTTCT ATGATGTGGT CAATATGCTC 
CTGAAGGACG CGCAGACTGG ATGCTGCCTT AATGATATGT TATCCAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004943994.1
CDS	143..7393
Translation	MAGARRELRVLLAARPGQARDSVRDAARVRLSAEQDALGSLLLRDGIYLESLGPGGGGRARSGVLPGAWADFVWERTAEYDPQAKRTKILALSESHDLFVYEFTREDGKCGPDPLHSCREGVLKKLLEANSVSLSSILSLRILSFKDNKCTLLLNSFIVMHLTFPGEHSSLETCDCFTLDLPPPVLERVTDINFCRGILFFLDSSGWIYVFDSSDGANLAHINVSLCQGGVQDGDRSPAITSPLTALRVSYDLSVAVIISSSSNACSVDLNVYFRQFPGHLICRRDSESLPLKPPEGVDEDDLASSDYSMELLSSPFRTDRSWKAHLSSLCSTIRKRRSRSPHLLCDMNLPWYQYFTHLEDHDPLIYETSEKALAIAPKAFPRVLSNPSQSAGTKLSDMGQQWKQILPVQLSQEVVKLECKSVTGAKALFVILTDDKALTLALWDFESQDVAHYQFGKNSVYVDCNEEVPLCLLLTECGLSLILFGLTQEEFLNRLMIYGSAGVVDSVCHLNGWGRCSIPVHALEAGLENRQLDTVDLFLKSKESLFNLSALRSEAQQSADGASCSFLKNLQELRPALNLLCSAIRENDMEPQSKHFSEQLLNLTLTFLSKQLEEIFVHTEEPDRYLQKAAEILTDYIIELRTFMKNYPRSQVNTTNVGSDSDEDLPAIEESQMWEKLTPEEVIAEAILSNKMPEAQIFFRMHRHPAQSLQELIRTGLDLVYGHLLKGNTREASALLKNMGFDVKQQLHKICFYTADKHVRDLLVKVLQEENYFSEKEKKMIDFVHQVESLYSESFQENKEMQSLSSVRNWRQEQDSSKHLATLESLMDHDGRRVPSREVRVMLNWAQCWDELLQDMILLPRKQSQGLATCNPEALWMYLTAQHDWLNISSWIEEFEPNHSLCGQSKWPPLAASIVDQHTLCSGYMRNEILNKLARNGIFVLSELEDFELLLQRLVSLGGVLQNPHPVPKYSAASGLDFHARFVLHCLEHNLQYMLYTYLEYYSLTPSNCPILDDKELHEAHPWFEFLVQCRGVASNPRDPKMIFQASLANAQILIPSNQASVSSMLLEGRTLLALATTMYAPGGIDQVLQNEDTEKPLKKVDPQLLKMALSPYPKLRAALFPPCTSHGILPPDISLYHLVQSLVPFDPSKLFGWQSTNTLAVSDASSDLPHFSSPELVNKYAVMERLDFLYYLRHGRPSFAFGTFLVQQLVKSKSPKQLIQQAGNEAYLLGLSFFNTPSVAAACVCFLELLGLSSLKLRVDIKVANMIFIYKTRNEETQHNQIRESLVEQLMRLADGEKAATEEVLKSLEEAFWDSIEHQGIKRTSSDSRQQWSLVMQFCKLHAMKLSTSYLKECAKSNDWLQFIIQAQLYSYKPDEVSSVLEGFAPIIQRHLMLALEKSPFLHAAAGAPEGSTGSRCADSLFQILFRCQESPNASCHLLAEGLREHAPILSVLAACSPDADIIHCLCVWIITSVDDVTRSEATKHIQSSVENHKWDLHDLSLIWKVLLRKQKCQTLIRGFQLFLKDSPLLHILEMYDLCMNCKRYYEAKTKLDEFQKHLTNLKTTDGAAASGVPVQWLESQALFLLELMMQQCRTEYELGKLLQLFAATDNLLLDGPYVKKLCALSETLRDSSVPINRSMLTRYTPETFQKECTSILEQLQEKGMFSLAREVAELAELPADSVVTQEILRDIRLLRDTGEWDRDQIRIKFWKKCNDSFVKNSISSKAASDFFLNQGNVVSESPVREKINSISERHLLLTLAGHWLAKDVSVALDKLEEIEKQIWICRITQQMLSTDEGPGKSGRSSCAAANGDFTFDNLAKEFSFSKLPALNTPNYLNVEGLELTGCQQTLAAEEEESLRFLVGCLLDEGSVHEASRVCRYFHFYSRDVSLVLHCRALAAGEADLDRLHADIQALVAIEKTQDSSASQQSRVPSTSSLEDWTHIADPSPDDQIVVSLKALTDECVHGRNYCRQELNCSYSEMSALDPEKVLRAILSSQQAERCRRAQAFITTQGLQPETVAELVAKEVVQELLASSEGKGQKQVSNPAAESQAFLQLAKLCQDHTLVGMKLLEKISSVPRGELSCITELLILAHNCFSLTCHMEGITRVLQAARLLTDEHLAPNDEYGLMVRLLTGIGRYNEMTYIFELLHEKHYFEVLMRKKLDPSGTLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAACIQLKLIESQPWEESLQDVANLKKLLMKALTLFIDAAESYSKDSCVRQALRCRRLTKLITLQLHFLSTGQSTMLINRNRQNLMECIVSLPRFYQAAVVAEAYEFVPDWAEVLYQQVIAKGDFSYLEEFKQQRLLKPAMFEEIAKKCKQHPPSDAALRNLKKLLAYCEDISTYYRLAYDNKFYDVVNMLLKDAQTGCCLNDMLSS

Target ORF information:

RefSeq Version	XM_004943937.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004943937.3

ORF Insert Sequence:

ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC 
CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG 
CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT 
GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC 
TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG 
GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG 
AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG 
CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT 
TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT 
TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA 
CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC 
ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT 
TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT 
CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG 
GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC 
AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT 
TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA 
GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT 
TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG 
CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT 
AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA 
CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC 
GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC 
TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC 
AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA 
GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA 
AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA 
GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG 
CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG 
CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA 
GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA 
CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT 
GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT 
GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC 
CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG 
GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG 
GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA 
CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA 
ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA 
GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT 
TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC 
AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC 
CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG 
ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC 
AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG 
TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT 
GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA 
GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT 
GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA 
GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA 
GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT 
CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA 
GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG 
TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG 
AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT 
CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT 
GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC 
CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC 
AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG 
TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG 
ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA 
GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA 
GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG 
CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA 
GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT 
CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC 
TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT 
GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA 
TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG 
TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA 
GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT 
TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT 
GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC 
AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT 
GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG 
GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG 
TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC 
CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG 
GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA 
TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT 
GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC 
TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG 
CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG 
CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA 
GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC 
GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG 
GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT 
ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA 
ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG 
CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC 
TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC 
GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG 
CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT 
TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG 
GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA 
GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA 
GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG 
GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGAACT GAACTGCTCC 
TACAGTGAGA TGTCAGCCCT TGACCCTGAG AAAGTGCTTC GAGCAATTCT GTCATCCCAG 
CAGGCAGAGC GCTGCAGGAG GGCTCAGGCG TTCATCACCA CGCAGGGCCT GCAGCCCGAA 
ACTGTGGCAG AGCTGGTGGC CAAAGAGGTC GTGCAGGAGT TACTGGCCTC TTCAGAAGGA 
AAAGGACAGA AGCAGGTTTC CAACCCTGCA GCTGAGAGCC AGGCGTTCCT GCAGCTCGCA 
AAGCTGTGCC AGGACCACAC GCTGGTGGGC ATGAAGCTGC TGGAGAAAAT TTCCTCTGTG 
CCACGTGGAG AACTGTCATG CATTACAGAA CTGCTGATTC TGGCCCATAA TTGCTTTAGT 
CTGACTTGCC ACATGGAAGG AATCACCAGA GTCCTGCAGG CAGCACGGCT GCTCACAGAT 
GAGCATTTGG CACCTAATGA TGAGTATGGA CTTATGGTTC GTCTTCTCAC TGGGATCGGC 
AGATACAATG AGATGACCTA CATCTTTGAG CTGCTGCACG AGAAGCATTA CTTTGAAGTG 
CTCATGAGGA AGAAGTTGGA CCCGAGTGGG ACACTGAAGA CAGCTCTGCT GGATTACATC 
AAGCGCTGCC GCCCAGGGGA CAGTGAGAAG CACAACATGA TTGCCCTTTG CTTCAGCATG 
TGCCGGGAAA TTGGAGAGAA TCACGAGGCT GCTGCTTGCA TACAGCTGAA ACTCATTGAA 
TCTCAGCCAT GGGAGGAGTC CCTTCAGGAT GTTGCAAACT TAAAGAAACT GCTGATGAAA 
GCTCTGACCC TCTTCATTGA TGCAGCAGAA AGTTACTCTA AGGACTCCTG TGTCCGCCAG 
GCGCTGCGCT GCCGCCGCCT CACAAAGCTG ATCACTCTGC AGCTGCATTT TCTGAGCACC 
GGTCAGAGCA CGATGCTGAT TAACAGGAAC AGGCAGAACC TCATGGAATG CATCGTGTCC 
CTGCCTCGAT TCTATCAGGC GGCGGTTGTG GCCGAGGCAT ATGAGTTTGT TCCAGATTGG 
GCTGAAGTTC TGTACCAACA AGTGATCGCT AAGGGAGACT TCAGCTACTT AGAAGAATTT 
AAACAGCAGA GACTGCTGAA GCCTGCCATG TTTGAGGAGA TTGCGAAGAA GTGTAAGCAG 
CACCCACCCA GCGATGCTGC TCTGAGGAAT CTGAAGAAGC TGCTCGCTTA CTGCGAGGAC 
ATCTCCACGT ACTACAGGCT GGCATATGAT AACAAGTTCT ATGATGTGGT CAATATGCTC 
CTGAAGGACG CGCAGACTGG ATGCTGCCTT AATGATATGT TATCCAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_004943939.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004943996.1 Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X4, mRNA.

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CloneID	OGa13357
Clone ID Related Accession (Same CDS sequence)	XM_004943939.2 , XM_004943939.3
Accession Version	XM_004943939.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	spatacsin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455925.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004943939.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC 
CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG 
CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT 
GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC 
TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG 
GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG 
AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG 
CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT 
TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT 
TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA 
CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC 
ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT 
TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT 
CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG 
GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC 
AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT 
TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA 
GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT 
TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG 
CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT 
AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA 
CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC 
GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC 
TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC 
AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA 
GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA 
AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA 
GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG 
CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG 
CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA 
GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA 
CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT 
GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT 
GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC 
CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG 
GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG 
GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA 
CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA 
ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA 
GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT 
TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC 
AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC 
CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG 
ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC 
AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG 
TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT 
GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA 
GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT 
GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA 
GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA 
GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT 
CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA 
GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG 
TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG 
AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT 
CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT 
GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC 
CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC 
AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG 
TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG 
ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA 
GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA 
GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG 
CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA 
GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT 
CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC 
TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT 
GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA 
TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG 
TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA 
GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT 
TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT 
GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC 
AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT 
GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG 
GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG 
TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC 
CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG 
GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA 
TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT 
GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC 
TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG 
CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG 
CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA 
GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC 
GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG 
GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT 
ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA 
ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG 
CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC 
TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC 
GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG 
CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT 
TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG 
GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA 
GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA 
GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG 
GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGTTCT GTGTCTGTAT 
GAACTTTCTA AGGTGCCAGA AACAAGTTGC AGAGAGCAGT ACTACCAGCC CTCGTGTGGA 
AACATGGCCA GATGCTCCTC AACTCCCCAG GCCCCTACCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004943996.1
CDS	104..6145
Translation	MAGARRELRVLLAARPGQARDSVRDAARVRLSAEQDALGSLLLRDGIYLESLGPGGGGRARSGVLPGAWADFVWERTAEYDPQAKRTKILALSESHDLFVYEFTREDGKCGPDPLHSCREGVLKKLLEANSVSLSSILSLRILSFKDNKCTLLLNSFIVMHLTFPGEHSSLETCDCFTLDLPPPVLERVTDINFCRGILFFLDSSGWIYVFDSSDGANLAHINVSLCQGGVQDGDRSPAITSPLTALRVSYDLSVAVIISSSSNACSVDLNVYFRQFPGHLICRRDSESLPLKPPEGVDEDDLASSDYSMELLSSPFRTDRSWKAHLSSLCSTIRKRRSRSPHLLCDMNLPWYQYFTHLEDHDPLIYETSEKALAIAPKAFPRVLSNPSQSAGTKLSDMGQQWKQILPVQLSQEVVKLECKSVTGAKALFVILTDDKALTLALWDFESQDVAHYQFGKNSVYVDCNEEVPLCLLLTECGLSLILFGLTQEEFLNRLMIYGSAGVVDSVCHLNGWGRCSIPVHALEAGLENRQLDTVDLFLKSKESLFNLSALRSEAQQSADGASCSFLKNLQELRPALNLLCSAIRENDMEPQSKHFSEQLLNLTLTFLSKQLEEIFVHTEEPDRYLQKAAEILTDYIIELRTFMKNYPRSQVNTTNVGSDSDEDLPAIEESQMWEKLTPEEVIAEAILSNKMPEAQIFFRMHRHPAQSLQELIRTGLDLVYGHLLKGNTREASALLKNMGFDVKQQLHKICFYTADKHVRDLLVKVLQEENYFSEKEKKMIDFVHQVESLYSESFQENKEMQSLSSVRNWRQEQDSSKHLATLESLMDHDGRRVPSREVRVMLNWAQCWDELLQDMILLPRKQSQGLATCNPEALWMYLTAQHDWLNISSWIEEFEPNHSLCGQSKWPPLAASIVDQHTLCSGYMRNEILNKLARNGIFVLSELEDFELLLQRLVSLGGVLQNPHPVPKYSAASGLDFHARFVLHCLEHNLQYMLYTYLEYYSLTPSNCPILDDKELHEAHPWFEFLVQCRGVASNPRDPKMIFQASLANAQILIPSNQASVSSMLLEGRTLLALATTMYAPGGIDQVLQNEDTEKPLKKVDPQLLKMALSPYPKLRAALFPPCTSHGILPPDISLYHLVQSLVPFDPSKLFGWQSTNTLAVSDASSDLPHFSSPELVNKYAVMERLDFLYYLRHGRPSFAFGTFLVQQLVKSKSPKQLIQQAGNEAYLLGLSFFNTPSVAAACVCFLELLGLSSLKLRVDIKVANMIFIYKTRNEETQHNQIRESLVEQLMRLADGEKAATEEVLKSLEEAFWDSIEHQGIKRTSSDSRQQWSLVMQFCKLHAMKLSTSYLKECAKSNDWLQFIIQAQLYSYKPDEVSSVLEGFAPIIQRHLMLALEKSPFLHAAAGAPEGSTGSRCADSLFQILFRCQESPNASCHLLAEGLREHAPILSVLAACSPDADIIHCLCVWIITSVDDVTRSEATKHIQSSVENHKWDLHDLSLIWKVLLRKQKCQTLIRGFQLFLKDSPLLHILEMYDLCMNCKRYYEAKTKLDEFQKHLTNLKTTDGAAASGVPVQWLESQALFLLELMMQQCRTEYELGKLLQLFAATDNLLLDGPYVKKLCALSETLRDSSVPINRSMLTRYTPETFQKECTSILEQLQEKGMFSLAREVAELAELPADSVVTQEILRDIRLLRDTGEWDRDQIRIKFWKKCNDSFVKNSISSKAASDFFLNQGNVVSESPVREKINSISERHLLLTLAGHWLAKDVSVALDKLEEIEKQIWICRITQQMLSTDEGPGKSGRSSCAAANGDFTFDNLAKEFSFSKLPALNTPNYLNVEGLELTGCQQTLAAEEEESLRFLVGCLLDEGSVHEASRVCRYFHFYSRDVSLVLHCRALAAGEADLDRLHADIQALVAIEKTQDSSASQQSRVPSTSSLEDWTHIADPSPDDQIVVSLKALTDECVHGRNYCRQVLCLYELSKVPETSCREQYYQPSCGNMARCSSTPQAPT

Target ORF information:

RefSeq Version	XM_004943939.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004943939.2

ORF Insert Sequence:

ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC 
CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG 
CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT 
GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC 
TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG 
GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG 
AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG 
CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT 
TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT 
TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA 
CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC 
ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT 
TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT 
CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG 
GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC 
AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT 
TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA 
GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT 
TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG 
CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT 
AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA 
CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC 
GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC 
TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC 
AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA 
GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA 
AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA 
GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG 
CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG 
CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA 
GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA 
CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT 
GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT 
GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC 
CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG 
GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG 
GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA 
CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA 
ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA 
GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT 
TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC 
AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC 
CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG 
ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC 
AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG 
TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT 
GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA 
GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT 
GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA 
GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA 
GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT 
CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA 
GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG 
TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG 
AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT 
CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT 
GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC 
CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC 
AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG 
TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG 
ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA 
GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA 
GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG 
CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA 
GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT 
CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC 
TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT 
GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA 
TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG 
TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA 
GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT 
TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT 
GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC 
AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT 
GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG 
GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG 
TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC 
CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG 
GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA 
TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT 
GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC 
TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG 
CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG 
CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA 
GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC 
GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG 
GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT 
ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA 
ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG 
CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC 
TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC 
GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG 
CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT 
TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG 
GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA 
GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA 
GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG 
GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGTTCT GTGTCTGTAT 
GAACTTTCTA AGGTGCCAGA AACAAGTTGC AGAGAGCAGT ACTACCAGCC CTCGTGTGGA 
AACATGGCCA GATGCTCCTC AACTCCCCAG GCCCCTACCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_004943939.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004943996.1 Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X6, mRNA.

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CloneID	OGa13357
Clone ID Related Accession (Same CDS sequence)	XM_004943939.2 , XM_004943939.3
Accession Version	XM_004943939.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	spatacsin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004943939.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC 
CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG 
CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT 
GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC 
TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG 
GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG 
AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG 
CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT 
TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT 
TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA 
CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC 
ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT 
TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT 
CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG 
GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC 
AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT 
TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA 
GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT 
TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG 
CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT 
AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA 
CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC 
GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC 
TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC 
AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA 
GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA 
AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA 
GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG 
CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG 
CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA 
GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA 
CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT 
GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT 
GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC 
CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG 
GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG 
GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA 
CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA 
ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA 
GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT 
TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC 
AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC 
CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG 
ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC 
AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG 
TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT 
GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA 
GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT 
GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA 
GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA 
GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT 
CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA 
GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG 
TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG 
AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT 
CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT 
GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC 
CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC 
AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG 
TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG 
ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA 
GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA 
GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG 
CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA 
GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT 
CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC 
TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT 
GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA 
TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG 
TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA 
GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT 
TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT 
GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC 
AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT 
GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG 
GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG 
TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC 
CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG 
GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA 
TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT 
GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC 
TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG 
CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG 
CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA 
GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC 
GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG 
GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT 
ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA 
ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG 
CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC 
TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC 
GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG 
CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT 
TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG 
GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA 
GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA 
GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG 
GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGTTCT GTGTCTGTAT 
GAACTTTCTA AGGTGCCAGA AACAAGTTGC AGAGAGCAGT ACTACCAGCC CTCGTGTGGA 
AACATGGCCA GATGCTCCTC AACTCCCCAG GCCCCTACCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004943996.1
CDS	143..6184
Translation	MAGARRELRVLLAARPGQARDSVRDAARVRLSAEQDALGSLLLRDGIYLESLGPGGGGRARSGVLPGAWADFVWERTAEYDPQAKRTKILALSESHDLFVYEFTREDGKCGPDPLHSCREGVLKKLLEANSVSLSSILSLRILSFKDNKCTLLLNSFIVMHLTFPGEHSSLETCDCFTLDLPPPVLERVTDINFCRGILFFLDSSGWIYVFDSSDGANLAHINVSLCQGGVQDGDRSPAITSPLTALRVSYDLSVAVIISSSSNACSVDLNVYFRQFPGHLICRRDSESLPLKPPEGVDEDDLASSDYSMELLSSPFRTDRSWKAHLSSLCSTIRKRRSRSPHLLCDMNLPWYQYFTHLEDHDPLIYETSEKALAIAPKAFPRVLSNPSQSAGTKLSDMGQQWKQILPVQLSQEVVKLECKSVTGAKALFVILTDDKALTLALWDFESQDVAHYQFGKNSVYVDCNEEVPLCLLLTECGLSLILFGLTQEEFLNRLMIYGSAGVVDSVCHLNGWGRCSIPVHALEAGLENRQLDTVDLFLKSKESLFNLSALRSEAQQSADGASCSFLKNLQELRPALNLLCSAIRENDMEPQSKHFSEQLLNLTLTFLSKQLEEIFVHTEEPDRYLQKAAEILTDYIIELRTFMKNYPRSQVNTTNVGSDSDEDLPAIEESQMWEKLTPEEVIAEAILSNKMPEAQIFFRMHRHPAQSLQELIRTGLDLVYGHLLKGNTREASALLKNMGFDVKQQLHKICFYTADKHVRDLLVKVLQEENYFSEKEKKMIDFVHQVESLYSESFQENKEMQSLSSVRNWRQEQDSSKHLATLESLMDHDGRRVPSREVRVMLNWAQCWDELLQDMILLPRKQSQGLATCNPEALWMYLTAQHDWLNISSWIEEFEPNHSLCGQSKWPPLAASIVDQHTLCSGYMRNEILNKLARNGIFVLSELEDFELLLQRLVSLGGVLQNPHPVPKYSAASGLDFHARFVLHCLEHNLQYMLYTYLEYYSLTPSNCPILDDKELHEAHPWFEFLVQCRGVASNPRDPKMIFQASLANAQILIPSNQASVSSMLLEGRTLLALATTMYAPGGIDQVLQNEDTEKPLKKVDPQLLKMALSPYPKLRAALFPPCTSHGILPPDISLYHLVQSLVPFDPSKLFGWQSTNTLAVSDASSDLPHFSSPELVNKYAVMERLDFLYYLRHGRPSFAFGTFLVQQLVKSKSPKQLIQQAGNEAYLLGLSFFNTPSVAAACVCFLELLGLSSLKLRVDIKVANMIFIYKTRNEETQHNQIRESLVEQLMRLADGEKAATEEVLKSLEEAFWDSIEHQGIKRTSSDSRQQWSLVMQFCKLHAMKLSTSYLKECAKSNDWLQFIIQAQLYSYKPDEVSSVLEGFAPIIQRHLMLALEKSPFLHAAAGAPEGSTGSRCADSLFQILFRCQESPNASCHLLAEGLREHAPILSVLAACSPDADIIHCLCVWIITSVDDVTRSEATKHIQSSVENHKWDLHDLSLIWKVLLRKQKCQTLIRGFQLFLKDSPLLHILEMYDLCMNCKRYYEAKTKLDEFQKHLTNLKTTDGAAASGVPVQWLESQALFLLELMMQQCRTEYELGKLLQLFAATDNLLLDGPYVKKLCALSETLRDSSVPINRSMLTRYTPETFQKECTSILEQLQEKGMFSLAREVAELAELPADSVVTQEILRDIRLLRDTGEWDRDQIRIKFWKKCNDSFVKNSISSKAASDFFLNQGNVVSESPVREKINSISERHLLLTLAGHWLAKDVSVALDKLEEIEKQIWICRITQQMLSTDEGPGKSGRSSCAAANGDFTFDNLAKEFSFSKLPALNTPNYLNVEGLELTGCQQTLAAEEEESLRFLVGCLLDEGSVHEASRVCRYFHFYSRDVSLVLHCRALAAGEADLDRLHADIQALVAIEKTQDSSASQQSRVPSTSSLEDWTHIADPSPDDQIVVSLKALTDECVHGRNYCRQVLCLYELSKVPETSCREQYYQPSCGNMARCSSTPQAPT

Target ORF information:

RefSeq Version	XM_004943939.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004943939.3

ORF Insert Sequence:

ATGGCGGGGG CGAGGCGGGA GCTGCGCGTG CTGCTGGCGG CCCGCCCGGG TCAGGCGCGG 
GACTCCGTGC GGGACGCGGC GCGGGTCCGG CTGAGCGCGG AGCAGGACGC GCTGGGCAGC 
CTGCTGCTCC GTGACGGGAT CTACCTGGAG TCCCTGGGGC CGGGCGGAGG CGGCCGGGCG 
CGGAGCGGCG TCCTGCCGGG AGCGTGGGCT GACTTCGTGT GGGAGAGAAC AGCAGAGTAT 
GATCCGCAAG CAAAGAGGAC AAAAATACTG GCTCTCAGTG AAAGTCACGA CCTGTTTGTC 
TATGAATTCA CCAGAGAAGA CGGAAAATGC GGCCCTGATC CCCTGCACAG CTGCAGGGAG 
GGTGTGCTGA AAAAGCTCCT GGAGGCCAAC AGCGTCAGTC TGTCTTCAAT TTTGTCTCTG 
AGGATACTGT CTTTTAAAGA CAACAAATGC ACGCTTCTGC TCAACAGCTT TATTGTTATG 
CATCTCACCT TTCCTGGAGA GCATTCATCA TTGGAGACGT GTGACTGCTT TACCCTTGAT 
TTGCCTCCAC CAGTGTTGGA AAGAGTAACA GATATAAACT TCTGCAGGGG GATTCTGTTT 
TTCTTGGATA GTTCTGGCTG GATCTACGTA TTTGATTCTT CTGATGGTGC AAATCTAGCA 
CACATCAACG TGTCACTCTG CCAGGGGGGT GTGCAGGATG GGGACAGGAG TCCCGCCATC 
ACATCACCGC TCACTGCACT CAGAGTGTCA TATGATCTCA GCGTTGCTGT GATCATCAGT 
TCTTCCAGCA ATGCCTGTTC TGTGGATCTA AACGTGTACT TCAGACAGTT CCCTGGGCAT 
CTGATTTGTA GAAGAGATTC TGAAAGCCTT CCACTGAAGC CACCTGAGGG AGTTGATGAG 
GATGACCTTG CTAGTTCTGA CTACAGTATG GAGCTGCTGT CCTCGCCTTT CCGCACAGAC 
AGGTCATGGA AGGCACACTT ATCCTCCCTG TGCAGCACAA TAAGGAAAAG AAGATCACGT 
TCCCCACATT TGCTATGTGA TATGAACTTA CCTTGGTATC AGTATTTTAC CCACCTTGAA 
GATCACGATC CCTTAATCTA TGAAACCTCT GAGAAGGCAC TGGCCATTGC TCCAAAGGCT 
TTTCCACGTG TCCTTTCCAA CCCATCGCAG AGTGCTGGCA CAAAGCTCAG TGATATGGGG 
CAGCAGTGGA AACAAATCCT CCCCGTACAG TTATCACAAG AAGTGGTGAA ACTGGAGTGT 
AAATCTGTGA CTGGAGCCAA AGCACTGTTT GTGATCCTAA CAGATGACAA GGCTTTGACA 
CTTGCTCTCT GGGATTTCGA GTCCCAGGAT GTAGCACATT ACCAGTTTGG CAAAAACAGC 
GTTTATGTGG ATTGTAATGA GGAGGTGCCA TTATGCCTGC TGCTGACAGA GTGTGGCCTC 
TCCTTAATTC TGTTTGGTTT AACTCAAGAG GAGTTTTTGA ATAGACTGAT GATCTATGGC 
AGCGCTGGAG TCGTAGACTC TGTATGTCAC CTCAATGGAT GGGGACGGTG TTCAATTCCA 
GTACATGCAT TGGAGGCTGG CTTGGAAAAT CGTCAGCTAG ACACAGTGGA CTTATTTTTA 
AAAAGCAAAG AAAGTCTTTT CAATCTCTCT GCGCTGCGCT CCGAAGCCCA GCAGTCTGCA 
GATGGTGCAT CCTGCTCTTT TCTGAAAAAT TTGCAAGAGC TGAGACCAGC TTTAAACTTG 
CTTTGCTCAG CAATCAGAGA GAACGACATG GAGCCGCAGA GCAAACACTT CTCAGAGCAG 
CTGCTGAACC TCACGCTGAC TTTCCTTAGC AAGCAGCTTG AAGAAATTTT TGTCCACACA 
GAGGAACCTG ATAGATACCT GCAGAAGGCT GCAGAGATTT TAACTGATTA CATCATTGAA 
CTTAGGACGT TTATGAAAAA CTATCCTCGA TCACAAGTAA ATACCACAAA TGTAGGGAGT 
GATTCTGATG AAGACCTGCC TGCAATAGAG GAAAGCCAGA TGTGGGAAAA ACTGACACCT 
GAGGAAGTCA TTGCTGAAGC CATCTTAAGC AACAAAATGC CAGAGGCCCA GATCTTCTTC 
CGAATGCACC GGCATCCTGC TCAGAGCCTT CAGGAACTCA TTCGGACAGG TTTGGATCTG 
GTCTATGGCC ATCTTCTGAA GGGCAATACC AGAGAGGCCT CAGCACTTCT GAAGAACATG 
GGTTTTGATG TGAAGCAGCA GTTGCATAAG ATATGTTTCT ACACGGCTGA TAAACACGTA 
CGAGATTTGC TGGTCAAAGT TTTACAAGAA GAAAATTATT TTTCTGAAAA AGAGAAGAAA 
ATGATAGACT TTGTGCATCA GGTTGAAAGC TTGTACTCGG AGTCCTTTCA AGAAAATAAA 
GAGATGCAAT CTCTTTCCAG TGTAAGGAAC TGGAGACAGG AACAAGACTC TTCCAAACAT 
TTGGCCACTC TGGAGTCATT GATGGATCAT GATGGACGCC GGGTGCCCAG CAGGGAGGTC 
AGGGTGATGC TGAACTGGGC TCAGTGCTGG GATGAGCTCC TTCAGGATAT GATTCTTCTC 
CCCAGAAAAC AAAGCCAAGG ACTTGCCACA TGTAACCCTG AGGCTCTTTG GATGTACCTG 
ACAGCCCAGC ATGATTGGCT TAATATCAGT TCATGGATTG AAGAATTTGA GCCCAACCAC 
AGCTTATGTG GACAAAGTAA ATGGCCACCC CTGGCTGCAA GCATTGTTGA CCAACACACG 
TTGTGTAGTG GCTACATGAG AAATGAAATA TTGAACAAGC TGGCCAGAAA TGGGATTTTT 
GTCCTTTCTG AATTGGAAGA CTTTGAACTT CTGCTCCAAA GATTGGTTTC CCTTGGAGGA 
GTACTGCAAA ATCCTCACCC TGTTCCAAAG TACAGTGCTG CGAGTGGTTT GGACTTCCAT 
GCTCGTTTTG TCCTTCACTG CTTAGAACAC AATTTGCAGT ATATGTTATA CACATACCTA 
GAATACTACA GTTTAACTCC TTCCAACTGC CCTATTTTGG ATGACAAGGA ACTGCATGAA 
GCACATCCCT GGTTTGAATT CCTCGTGCAG TGCCGGGGGG TTGCCAGTAA CCCACGAGAT 
CCAAAGATGA TTTTTCAGGC GAGTCTAGCA AATGCTCAGA TCTTGATTCC TAGTAATCAA 
GCCAGCGTGA GTAGCATGCT GCTGGAGGGA CGTACACTGT TAGCACTTGC TACCACCATG 
TATGCACCTG GAGGCATTGA TCAGGTTCTT CAGAATGAAG ATACAGAGAA ACCACTAAAG 
AAAGTTGATC CTCAGCTCTT AAAAATGGCA TTGAGTCCTT ACCCCAAACT CAGAGCTGCT 
CTTTTTCCCC CGTGCACCTC CCATGGAATT TTGCCACCTG ACATTTCCCT TTATCATCTT 
GTTCAGTCAT TAGTGCCGTT TGATCCCAGT AAGTTATTTG GCTGGCAGTC AACAAATACC 
CTTGCTGTAT CAGATGCATC AAGTGACCTT CCTCATTTCT CATCCCCTGA ACTGGTGAAC 
AAATATGCTG TAATGGAACG ACTGGACTTT TTGTACTACC TGCGCCATGG ACGCCCATCG 
TTTGCCTTTG GTACATTTTT GGTCCAGCAG TTGGTCAAGA GCAAAAGCCC CAAACAGTTG 
ATTCAGCAAG CAGGAAATGA AGCCTATCTT CTAGGCCTTT CTTTCTTCAA TACGCCTTCA 
GTAGCAGCTG CCTGTGTGTG CTTTCTGGAG CTGCTTGGGC TCAGCAGCCT CAAACTGAGA 
GTTGATATTA AGGTTGCAAA CATGATATTC ATTTACAAAA CCAGAAATGA AGAAACTCAG 
CACAATCAAA TAAGGGAATC TCTGGTTGAA CAGCTAATGA GGTTGGCTGA TGGTGAAAAA 
GCAGCAACAG AAGAAGTTCT TAAATCCTTA GAAGAAGCCT TTTGGGATTC AATTGAGCAT 
CAAGGAATAA AGAGGACATC CAGTGACTCC AGACAGCAGT GGTCTTTGGT GATGCAGTTC 
TGCAAACTCC ATGCCATGAA ACTGAGCACC TCATACCTAA AGGAGTGTGC TAAATCCAAT 
GACTGGCTGC AGTTCATCAT CCAAGCCCAG CTGTACAGCT ACAAGCCAGA TGAGGTCTCA 
TCTGTCCTTG AAGGCTTCGC TCCCATTATA CAGCGTCACC TGATGTTGGC ACTGGAGAAG 
TCACCGTTTC TGCACGCTGC TGCGGGGGCT CCTGAGGGCA GCACTGGGAG CAGATGTGCA 
GACAGTCTCT TCCAAATCCT TTTCCGGTGT CAGGAGAGCC CCAATGCTTC GTGTCACCTT 
TTGGCTGAAG GACTGAGAGA ACATGCTCCC ATTCTGAGTG TTTTAGCAGC GTGTTCCCCT 
GATGCTGACA TTATTCACTG TCTCTGTGTC TGGATAATTA CCAGTGTGGA TGATGTTACC 
AGATCTGAAG CGACAAAACA TATTCAGAGC TCAGTAGAAA ATCACAAGTG GGATCTTCAT 
GACCTGTCGC TTATCTGGAA AGTGCTTTTA AGAAAGCAAA AATGTCAAAC ACTTATAAGG 
GGATTCCAGC TCTTCTTAAA GGATTCCCCT TTACTGCATA TACTGGAGAT GTACGATCTG 
TGCATGAACT GTAAAAGGTA CTATGAAGCC AAAACCAAAC TGGACGAGTT TCAGAAACAC 
CTCACAAATC TGAAGACCAC CGATGGAGCG GCAGCTTCAG GAGTCCCTGT GCAATGGTTG 
GAGTCACAGG CCTTGTTTCT TCTTGAACTG ATGATGCAGC AGTGTAGGAC AGAATATGAA 
TTGGGAAAGC TCTTACAGCT GTTTGCTGCA ACAGACAACC TTCTCCTTGA TGGCCCTTAT 
GTGAAGAAAC TGTGTGCGCT CAGTGAGACC CTGAGGGATT CCTCCGTGCC CATTAATCGC 
TCCATGCTGA CCAGGTACAC CCCAGAAACG TTTCAGAAGG AGTGCACATC GATTCTGGAG 
CAGCTCCAGG AGAAAGGAAT GTTCTCTCTG GCTCGGGAGG TGGCAGAGCT GGCTGAACTG 
CCTGCGGACA GCGTGGTTAC CCAAGAGATT CTGAGGGATA TACGTCTGTT AAGAGACACA 
GGAGAGTGGG ATCGAGATCA GATAAGAATT AAGTTCTGGA AAAAGTGTAA CGACAGCTTC 
GTGAAAAATT CCATCTCCAG CAAAGCTGCA TCGGATTTCT TTTTGAATCA GGGCAATGTG 
GTGTCTGAAT CCCCAGTTCG TGAGAAGATA AACAGCATTT CGGAGAGACA TCTGCTGCTT 
ACCCTGGCTG GCCATTGGCT CGCCAAGGAT GTCTCGGTGG CATTGGACAA ATTGGAGGAA 
ATAGAGAAAC AGATCTGGAT TTGTCGCATC ACACAACAAA TGTTATCCAC AGATGAGGGG 
CCAGGCAAGT CGGGACGCTC CTCCTGTGCT GCAGCGAATG GGGATTTTAC TTTTGATAAC 
TTGGCCAAGG AGTTTTCTTT TTCAAAGCTG CCTGCTCTGA ACACACCAAA CTACTTAAAC 
GTGGAGGGTC TTGAATTGAC GGGGTGTCAG CAGACCTTGG CTGCGGAGGA GGAGGAGTCG 
CTCAGGTTTC TGGTCGGATG CCTTCTCGAT GAAGGAAGCG TGCACGAGGC CAGCCGCGTT 
TGTCGCTATT TCCATTTCTA CAGCAGAGAC GTTTCACTGG TGTTGCACTG CAGAGCCCTG 
GCCGCAGGGG AGGCTGACCT GGACAGACTG CACGCAGATA TTCAGGCTCT TGTAGCAATA 
GAGAAGACAC AGGACAGCAG TGCATCGCAG CAGAGCAGAG TCCCGAGCAC ATCGAGCTTA 
GAGGACTGGA CACACATTGC AGATCCATCG CCGGATGATC AGATTGTCGT CAGTCTGAAG 
GCCCTCACAG ATGAATGTGT CCATGGGAGA AACTACTGCA GACAGGTTCT GTGTCTGTAT 
GAACTTTCTA AGGTGCCAGA AACAAGTTGC AGAGAGCAGT ACTACCAGCC CTCGTGTGGA 
AACATGGCCA GATGCTCCTC AACTCCCCAG GCCCCTACCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SPG11
Entrez Gene ID	415572
Full Name	spastic paraplegia 11 (autosomal recessive)
General protein information	Preferred Names spastic paraplegia 11 (autosomal recessive) Names spatacsin
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Rattus norvegicus (Norway rat)	Spg11	NP_001178500.1
Homo sapiens (human)	SPG11	NP_079413.3
Canis lupus familiaris (dog)	SPG11	XP_003640073.2
Bos taurus (cattle)	SPG11	XP_002691138.2
Mus musculus (house mouse)	Spg11	NP_663506.2
	LOC712221	XP_002808495.1
Gallus gallus (chicken)	SPG11	XP_003641879.2
Danio rerio (zebrafish)	spg11	NP_001181923.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SPG11 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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