P3H1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	P3H1
Entrez Gene ID	414142
Full Name	prolyl 3-hydroxylase 1
Synonyms	LEPRE1
General protein information	Preferred Names prolyl 3-hydroxylase 1 Names prolyl 3-hydroxylase 1 leprecan 1 leprecan-1 homolog leucine proline-enriched proteoglycan (leprecan) 1 leucine- and proline-enriched proteoglycan 1 homolog
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001001529.1	NP_001001529.1	prolyl 3-hydroxylase 1 precursor

Pathways from BioSystems

Homologies

	LEPRE1	XP_001088904.1
Mus musculus (house mouse)	Lepre1	NP_062757.2
Homo sapiens (human)	LEPRE1	NP_071751.3
Rattus norvegicus (Norway rat)	Lepre1	NP_446119.1
Gallus gallus (chicken)	LEPRE1	NP_001001529.1
Danio rerio (zebrafish)	lepre1	XP_002663865.1
Xenopus tropicalis (tropical clawed frog)	lepre1	NP_001090700.1
Pan troglodytes (chimpanzee)	LEPRE1	XP_003308086.1
Canis lupus familiaris (dog)	LEPRE1	XP_848570.2
Bos taurus (cattle)	LEPRE1	NP_001096761.1

Gene Ontology
Bibliography

Related articles in PubMed

An additional function of the rough endoplasmic reticulum protein complex prolyl 3-hydroxylase 1?cartilage-associated protein?cyclophilin B: the CXXXC motif reveals disulfide isomerase activity in vitro.
Ishikawa Y, B?chinger HP
The Journal of biological chemistry288(44)31437-46(2013 Nov)

Biochemical characterization of the prolyl 3-hydroxylase 1.cartilage-associated protein.cyclophilin B complex.
Ishikawa Y, Wirz J, Vranka JA, Nagata K, B?chinger HP
The Journal of biological chemistry284(26)17641-7(2009 Jun)

CRTAP is required for prolyl 3- hydroxylation and mutations cause recessive osteogenesis imperfecta.
Morello R, Bertin TK, Chen Y, Hicks J, Tonachini L, Monticone M, Castagnola P, Rauch F, Glorieux FH, Vranka J, B?chinger HP, Pace JM, Schwarze U, Byers PH, Weis M, Fernandes RJ, Eyre DR, Yao Z, Boyce BF, Lee B
Cell127(2)291-304(2006 Oct)

Prolyl 3-hydroxylase 1, enzyme characterization and identification of a novel family of enzymes.
Vranka JA, Sakai LY, B?chinger HP
The Journal of biological chemistry279(22)23615-21(2004 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

An additional function of the rough endoplasmic reticulum protein complex prolyl 3-hydroxylase 1.cartilage-associated protein.cyclophilin B: the CXXXC motif reveals disulfide isomerase activity in vitro.
Title: An additional function of the rough endoplasmic reticulum protein complex prolyl 3-hydroxylase 1?cartilage-associated protein?cyclophilin B: the CXXXC motif reveals disulfide isomerase activity in vitro.

The following P3H1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the P3H1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa00212	NM_001001529.1 Latest version!	Gallus gallus prolyl 3-hydroxylase 1 (P3H1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

P3H1 ( NM_001001529.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001001529.1 Gallus gallus prolyl 3-hydroxylase 1 (P3H1), mRNA.

Price & Availability↑

CloneID	OGa00212
Clone ID Related Accession (Same CDS sequence)	NM_001001529.1
Accession Version	NM_001001529.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Gallus gallus(chicken)
Product	prolyl 3-hydroxylase 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY463528.1. On Aug 30, 2004 this sequence version replaced XM_417657.1. ##Evidence-Data-START## Transcript exon combination :: AY463528.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCGCTGC TGCTGCCGCT GCTGCCGCTG CTGGTCTGGG CGGCGGGACC GCCGGGACCG 
CTGGGACCGC CGGAGCACTC CGAGCCCCCT CTGCCGGCCG AGCCCCCGGA CGCGCTCTTC 
GCTGCCGGCG CCGAGGCGTA CGCGCGGGGG GACTGGCCCG CCGTGGTGCT GCAGATGGAG 
CGGGCGCTGC GGGCCCGCGC CGCTATCCGC GCTCGATCGG TTCGGTGCCG CCTGCGCTGC 
GCCAATGCCA CCGCGGTGGT ACCGACGGAT GGACTCGAGC CGACTCTCCA CGATCTGCTG 
TTCTTTCGGG GGCTGCTGCG GCGCGCCGCC TGTCTGCGGG GCTGCGGGCC CACCCAGCCC 
TCCCGGTACC GCCTGGGCGA GGAGCTGGAG CGAGAGTTCC GCAAGCGGAG CCCCTACAAC 
TACCTCCAGG TCGCCTACTT CAAGATCAAT AAGGTGGCGA AGGCTGTGGC TGCAGCCCAC 
ACCTTCTTTG TGGCCAACCC CGAGCACGTG GAGATGAAGC AGAACCTGGA GTACTACCAG 
ATGATGGCGG GCGTCAGGGA GTCCGACTTC GCTGACCTGG AGGCCAGGCC CCACATGACA 
GAGTTCCGCC TGGGTGTCAG GTTCTACAGC GAGGAGCAGC CGGCTGCTGC TGTCCTGCAC 
CTGGAGAAGG CACTGCAGGA ATACTTTGTG GCAGACACCG AGTGCCGTGC TCTCTGTGAG 
GGGCCCTATG ACTATGAAGG CTACAACTAC CTGGAGTACA ACGCAGACCT GTTCCAGGCC 
ATGACAGATC ACTACATGCA GGTGCTGAGC TGCAAGCAGG GCTGCGTCAC AGAGCTCGCC 
TCACAGCCTG GCCGGGAGAA GCCCCTGGAG GATTTCCTGC CCTCACATTT CAACTACTTG 
CAGTTTGCCT ACTACAACAA TGGGAATTAT GAAAAAGCGA TTGAATGTGC CAAAACATAC 
TTGCTCTTCT TCCCAAATGA TGAGGTGATG AACCAGAATT TGGCCTATTA CACTGCTGTC 
CTGGGAGAAG ACCTGGCCAG GCCCATTGAG CCCCGAAAGG AAATCCAGGC ATACCGCCAG 
CGAAGCCTCA TGGAGAAGGA GCTGCTCTTC TTCAGCTACG ACGTCTTTGG GATCCCATTT 
GTGGACCCGG ACACGTGGAC GCCCGAAGAG GTGATACCAA AGAGGCTGCG AGAGAAACAA 
AAGGTGGAGC GGGAGACAGC GGCGCGCATC TCCGAGGAGA TCGGCAACCT GATGAAGGAG 
ATCGAGACGC TGGTGGAGGA GAAGGCGAAG GAGTCTGCAG AAATGAGCAA GTTCATCCGA 
GAAGGTGGCC CCTTGGTGTA TGAGGGAGCC AGCGTCACCA TGAACTCCAA AAGCCTGAAC 
GGGTCTCAGC GCGTGGTGGT GGACGGAGTC CTCTCAGCTG AGGAGTGCCG GGAGCTGCAG 
AGACTCACCA ACGCAGCTGC CTCGGCAGGG GATGGCTATC GTGGGAAGAC ATCGCCTCAC 
ACCCCCAGTG AGACCTTCTA CGGTGTCACC GTCCTGAAGG CCCTCAAGCT GGGCCAGGAG 
GGCAAGGTCC CCCTGCAGAG TGCCCACCTC TACTACAACG TGACGGAGAA GGTGCGGCAC 
ATGATGGAGT CCTACTTCCG CCTGGAGGTC CCGCTCCATT TCTCCTACTC ACACCTGGTG 
TGCCGCACAG CCATCGATGA GAAGCAAGAA GGCCGGAGTG ACAACAGCCA CGAGGTGCAT 
GTGGACAACT GCATCCTCAA CGCGGAGGCC CTGGTGTGTG TGAAGGAACC ACCAGCCTAC 
ACCTTCCGGG ATTACAGTGC CATCCTCTAC CTCAATGGGG ACTTTGAAGG GGGAGCTTTC 
TACTTCACGG AGCTGGATGC CAAGACGCAG ACTGCAGAGG TGCAGCCCCA GTGTGGCCGT 
GCCGTGGGCT TCTCCTCTGG CTCAGAGAAC CCCCACGGGG TGAAGGCTGT GACCAAAGGC 
CAACGCTGTG CCATTGCCCT CTGGTTCACC CTGGACCCAC GGCACAGTGA GCGGGAGCGC 
GTGCAGGCGG ACGATTTGGT GAAGATGCTT TTCAGGACGG AAGAGGTGGA TTTGCTGCAG 
GAGACGAGCG CGGAGCAGGA GCCAACGGCT GCCACCAGCA CTGCTGGCTT GCATGCAGCA 
GGGAAGGACG AACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001529.1
CDS	1..2178
Translation	MALLLPLLPLLVWAAGPPGPLGPPEHSEPPLPAEPPDALFAAGAEAYARGDWPAVVLQMERALRARAAIRARSVRCRLRCANATAVVPTDGLEPTLHDLLFFRGLLRRAACLRGCGPTQPSRYRLGEELEREFRKRSPYNYLQVAYFKINKVAKAVAAAHTFFVANPEHVEMKQNLEYYQMMAGVRESDFADLEARPHMTEFRLGVRFYSEEQPAAAVLHLEKALQEYFVADTECRALCEGPYDYEGYNYLEYNADLFQAMTDHYMQVLSCKQGCVTELASQPGREKPLEDFLPSHFNYLQFAYYNNGNYEKAIECAKTYLLFFPNDEVMNQNLAYYTAVLGEDLARPIEPRKEIQAYRQRSLMEKELLFFSYDVFGIPFVDPDTWTPEEVIPKRLREKQKVERETAARISEEIGNLMKEIETLVEEKAKESAEMSKFIREGGPLVYEGASVTMNSKSLNGSQRVVVDGVLSAEECRELQRLTNAAASAGDGYRGKTSPHTPSETFYGVTVLKALKLGQEGKVPLQSAHLYYNVTEKVRHMMESYFRLEVPLHFSYSHLVCRTAIDEKQEGRSDNSHEVHVDNCILNAEALVCVKEPPAYTFRDYSAILYLNGDFEGGAFYFTELDAKTQTAEVQPQCGRAVGFSSGSENPHGVKAVTKGQRCAIALWFTLDPRHSERERVQADDLVKMLFRTEEVDLLQETSAEQEPTAATSTAGLHAAGKDEL

Target ORF information:

RefSeq Version	NM_001001529.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus prolyl 3-hydroxylase 1 (P3H1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001529.1

ORF Insert Sequence:

ATGGCGCTGC TGCTGCCGCT GCTGCCGCTG CTGGTCTGGG CGGCGGGACC GCCGGGACCG 
CTGGGACCGC CGGAGCACTC CGAGCCCCCT CTGCCGGCCG AGCCCCCGGA CGCGCTCTTC 
GCTGCCGGCG CCGAGGCGTA CGCGCGGGGG GACTGGCCCG CCGTGGTGCT GCAGATGGAG 
CGGGCGCTGC GGGCCCGCGC CGCTATCCGC GCTCGATCGG TTCGGTGCCG CCTGCGCTGC 
GCCAATGCCA CCGCGGTGGT ACCGACGGAT GGACTCGAGC CGACTCTCCA CGATCTGCTG 
TTCTTTCGGG GGCTGCTGCG GCGCGCCGCC TGTCTGCGGG GCTGCGGGCC CACCCAGCCC 
TCCCGGTACC GCCTGGGCGA GGAGCTGGAG CGAGAGTTCC GCAAGCGGAG CCCCTACAAC 
TACCTCCAGG TCGCCTACTT CAAGATCAAT AAGGTGGCGA AGGCTGTGGC TGCAGCCCAC 
ACCTTCTTTG TGGCCAACCC CGAGCACGTG GAGATGAAGC AGAACCTGGA GTACTACCAG 
ATGATGGCGG GCGTCAGGGA GTCCGACTTC GCTGACCTGG AGGCCAGGCC CCACATGACA 
GAGTTCCGCC TGGGTGTCAG GTTCTACAGC GAGGAGCAGC CGGCTGCTGC TGTCCTGCAC 
CTGGAGAAGG CACTGCAGGA ATACTTTGTG GCAGACACCG AGTGCCGTGC TCTCTGTGAG 
GGGCCCTATG ACTATGAAGG CTACAACTAC CTGGAGTACA ACGCAGACCT GTTCCAGGCC 
ATGACAGATC ACTACATGCA GGTGCTGAGC TGCAAGCAGG GCTGCGTCAC AGAGCTCGCC 
TCACAGCCTG GCCGGGAGAA GCCCCTGGAG GATTTCCTGC CCTCACATTT CAACTACTTG 
CAGTTTGCCT ACTACAACAA TGGGAATTAT GAAAAAGCGA TTGAATGTGC CAAAACATAC 
TTGCTCTTCT TCCCAAATGA TGAGGTGATG AACCAGAATT TGGCCTATTA CACTGCTGTC 
CTGGGAGAAG ACCTGGCCAG GCCCATTGAG CCCCGAAAGG AAATCCAGGC ATACCGCCAG 
CGAAGCCTCA TGGAGAAGGA GCTGCTCTTC TTCAGCTACG ACGTCTTTGG GATCCCATTT 
GTGGACCCGG ACACGTGGAC GCCCGAAGAG GTGATACCAA AGAGGCTGCG AGAGAAACAA 
AAGGTGGAGC GGGAGACAGC GGCGCGCATC TCCGAGGAGA TCGGCAACCT GATGAAGGAG 
ATCGAGACGC TGGTGGAGGA GAAGGCGAAG GAGTCTGCAG AAATGAGCAA GTTCATCCGA 
GAAGGTGGCC CCTTGGTGTA TGAGGGAGCC AGCGTCACCA TGAACTCCAA AAGCCTGAAC 
GGGTCTCAGC GCGTGGTGGT GGACGGAGTC CTCTCAGCTG AGGAGTGCCG GGAGCTGCAG 
AGACTCACCA ACGCAGCTGC CTCGGCAGGG GATGGCTATC GTGGGAAGAC ATCGCCTCAC 
ACCCCCAGTG AGACCTTCTA CGGTGTCACC GTCCTGAAGG CCCTCAAGCT GGGCCAGGAG 
GGCAAGGTCC CCCTGCAGAG TGCCCACCTC TACTACAACG TGACGGAGAA GGTGCGGCAC 
ATGATGGAGT CCTACTTCCG CCTGGAGGTC CCGCTCCATT TCTCCTACTC ACACCTGGTG 
TGCCGCACAG CCATCGATGA GAAGCAAGAA GGCCGGAGTG ACAACAGCCA CGAGGTGCAT 
GTGGACAACT GCATCCTCAA CGCGGAGGCC CTGGTGTGTG TGAAGGAACC ACCAGCCTAC 
ACCTTCCGGG ATTACAGTGC CATCCTCTAC CTCAATGGGG ACTTTGAAGG GGGAGCTTTC 
TACTTCACGG AGCTGGATGC CAAGACGCAG ACTGCAGAGG TGCAGCCCCA GTGTGGCCGT 
GCCGTGGGCT TCTCCTCTGG CTCAGAGAAC CCCCACGGGG TGAAGGCTGT GACCAAAGGC 
CAACGCTGTG CCATTGCCCT CTGGTTCACC CTGGACCCAC GGCACAGTGA GCGGGAGCGC 
GTGCAGGCGG ACGATTTGGT GAAGATGCTT TTCAGGACGG AAGAGGTGGA TTTGCTGCAG 
GAGACGAGCG CGGAGCAGGA GCCAACGGCT GCCACCAGCA CTGCTGGCTT GCATGCAGCA 
GGGAAGGACG AACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An additional function of the rough endoplasmic reticulum protein complex prolyl 3-hydroxylase 1?cartilage-associated protein?cyclophilin B: the CXXXC motif reveals disulfide isomerase activity in vitro.
The Journal of biological chemistry288(44)31437-46(2013 Nov)
Ishikawa Y,B?chinger HP

Biochemical characterization of the prolyl 3-hydroxylase 1.cartilage-associated protein.cyclophilin B complex.
The Journal of biological chemistry284(26)17641-7(2009 Jun)
Ishikawa Y,Wirz J,Vranka JA,Nagata K,B?chinger HP

CRTAP is required for prolyl 3- hydroxylation and mutations cause recessive osteogenesis imperfecta.
Cell127(2)291-304(2006 Oct)
Morello R,Bertin TK,Chen Y,Hicks J,Tonachini L,Monticone M,Castagnola P,Rauch F,Glorieux FH,Vranka J,B?chinger HP,Pace JM,Schwarze U,Byers PH,Weis M,Fernandes RJ,Eyre DR,Yao Z,Boyce BF,Lee B

Prolyl 3-hydroxylase 1, enzyme characterization and identification of a novel family of enzymes.
The Journal of biological chemistry279(22)23615-21(2004 May)
Vranka JA,Sakai LY,B?chinger HP

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P3H1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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