EGFR cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	EGFR
Entrez Gene ID	396494
Full Name	epidermal growth factor receptor
Synonyms	c-erbB
General protein information	Preferred Names epidermal growth factor receptor Names epidermal growth factor receptor CER epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015281330.1	XP_015136816.1	epidermal growth factor receptor isoform X3
XM_015281328.2	XP_015136814.1	epidermal growth factor receptor isoform X3
NM_205497.2	NP_990828.2	epidermal growth factor receptor precursor
XM_025147075.1	XP_025002843.1	epidermal growth factor receptor isoform X1
XM_025147076.1	XP_025002844.1	epidermal growth factor receptor isoform X2
XM_015281328.1	XP_015136814.1	epidermal growth factor receptor isoform X1
XM_015281329.1	XP_015136815.1	epidermal growth factor receptor isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	EGFR	XP_533073.3
Pan troglodytes (chimpanzee)	EGFR	XP_519102.3
Bos taurus (cattle)	EGFR	XP_002696936.2
Mus musculus (house mouse)	Egfr	NP_997538.1
Gallus gallus (chicken)	EGFR	NP_990828.2
Danio rerio (zebrafish)	egfra	NP_919405.1
	EGFR	XP_001107305.1
Rattus norvegicus (Norway rat)	Egfr	NP_113695.1
Homo sapiens (human)	EGFR	NP_005219.2
Xenopus tropicalis (tropical clawed frog)	egfr	XP_002939960.2

Gene Ontology
Bibliography

Related articles in PubMed

Pecenka V, Pajer P, Karafiat V, Kasparova P, Dudlova J, Dvorak M
Journal of virology91(20)(2017 Oct)

DNA methylation down-regulates EGFR expression in chickens.
Luo J, Chang S, Zhang H, Li B, Song J
Avian diseases57(2 Suppl)366-71(2013 Jun)

EGFR signaling is required for regenerative proliferation in the cochlea: conservation in birds and mammals.
White PM, Stone JS, Groves AK, Segil N
Developmental biology363(1)191-200(2012 Mar)

Interleukin-6 promotes 2-deoxyglucose uptake through p44/42 MAPKs activation via Ca2+/PKC and EGF receptor in primary cultured chicken hepatocytes.
Suh HN, Lee YJ, Han HJ
Journal of cellular physiology218(3)643-52(2009 Mar)

Studies on epidermal growth factor receptor signaling in vertebrate limb patterning.
Omi M, Fisher M, Maihle NJ, Dealy CN
Developmental dynamics : an official publication of the American Association of Anatomists233(2)288-300(2005 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data describe the mechanisms of oncogenesis induced in chickens by the myeloblastosis-associated virus 2 retrovirus; analyses identified HRAS, EGFR, MET, and RON as insertionally mutagenized driver genes and characterized the mechanisms of their tumorigenic activation

Epidermal growth factor receptor (EGFR) expression was significantly down-regulated after Marek's disease virus infection. The methylation level of the EGFR promoter was significantly increased at 21 days postinfection.
Title: DNA methylation down-regulates EGFR expression in chickens.

Data suggest that a conserved mechanism involving EGFR signaling governs proliferation of auditory supporting cells in birds and mammals and may represent a target for future hair cell regeneration strategies.
Title: EGFR signaling is required for regenerative proliferation in the cochlea: conservation in birds and mammals.

IL-6 stimulates the 2-deoxyglucose uptake through p44/42 MAPKs activation via Ca(2+)/PKC and EGF receptor in primary cultured chicken hepatocytes.
Title: Interleukin-6 promotes 2-deoxyglucose uptake through p44/42 MAPKs activation via Ca2+/PKC and EGF receptor in primary cultured chicken hepatocytes.

potential roles for epidermal growth factor receptor signaling in anterior-posterior and dorsal-ventral patterning, apical ectodermal ridges formation, and cell survival during limb morphogenesis
Title: Studies on epidermal growth factor receptor signaling in vertebrate limb patterning.

The following EGFR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EGFR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa145765	XM_025147075.1 Latest version!	Gallus gallus epidermal growth factor receptor (EGFR), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa145766	XM_025147076.1 Latest version!	Gallus gallus epidermal growth factor receptor (EGFR), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa31512	XM_015281328.2 Latest version!	Gallus gallus epidermal growth factor receptor (EGFR), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa31513	XM_015281329.1 Latest version!	Gallus gallus epidermal growth factor receptor (EGFR), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa31514	XM_015281330.1 Latest version!	Gallus gallus epidermal growth factor receptor (EGFR), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa00162	NM_205497.2 Latest version!	Gallus gallus epidermal growth factor receptor (EGFR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
OGa31512	XM_015281328.1	Gallus gallus epidermal growth factor receptor (EGFR), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EGFR ( XM_025147075.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025002843.1 Gallus gallus epidermal growth factor receptor (EGFR), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa145765
Clone ID Related Accession (Same CDS sequence)	XM_025147075.1
Accession Version	XM_025147075.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	epidermal growth factor receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGTAC GCAGCCCCCT GTCCGCCTCT GGGCCTCGCG GGGCCGCTGT CCTGGTGCTA 
CTGCTGCTGC TGCTGGGGCG CGTCGCGCTG TGTTCCGCCG TGGAGGAGAA GAAAGTTTGT 
CAAGGGACAA ATAACAAGTT GACCCAGCTG GGGCACGTGG AAGACCATTT CACCAGCCTG 
CAGAGAATGT ACAACAACTG CGAAGTGGTA CTGAGTAACC TGGAGATTAC CTACGTGGAG 
CATAATCGCG ATCTCACCTT CCTTAAGACC ATACAGGAGG TTGCAGGCTA TGTGCTCATT 
GCGCTTAACA TGGTGGACGT CATTCCCTTA GAAAACCTCC AGATTATCCG AGGGAATGTG 
CTTTATGACA ACTCTTTTGC CCTGGCAGTT TTATCCAATT ACCACATGAA TAAAACCCAG 
GGACTTCGAG AGCTGCCAAT GAAACGGCTA TCAGAAATTC TCAATGGAGG TGTTAAAATC 
AGCAACAACC CCAAACTGTG CAACATGGAC ACTGTTCTCT GGAATGACAT CATTGATACA 
AGCAGGAAGC CTCTCACAGT ACTTGACTTT GCAAGCAATC TTTCTTCTTC TTGCACCCAT 
TGCCCCTTGT CCTATCACTG GATGTCACTG AGAGGAGCCT GGATCCTTGA GCCTTGTCCA 
AAATGCCATC CGAACTGCAC AGAAGACCAC TGCTGGGGTG CTGGTGAACA GAACTGCCAG 
ACTTTAACAA AAGTCATCTG TGCCCAGCAA TGCTCTGGCC GGTGCAGAGG AAAGGTGCCC 
AGTGACTGCT GCCACAATCA GTGTGCTGCA GGGTGCACAG GACCTCGGGA GAGTGACTGC 
CTGGCATGCC GCAAGTTTCG GGATGATGCT ACCTGCAAGG ACACATGTCC CCCACTGGTC 
CTCTATAACC CCACCACCTA TCAAATGGAT GTCAACCCCG AGGGAAAATA CAGCTTTGGA 
GCCACTTGTG TGAGGGAATG TCCACACAAC TATGTGGTGA CAGATCATGG CTCCTGCGTT 
CGCTCGTGTA ATACTGATAC TTACGAAGTG GAAGAAAATG GTGTTCGGAA GTGTAAAAAA 
TGTGATGGGC TATGTAGCAA AGTGTGCAAT GGCATTGGAA TAGGTGAACT TAAAGGGATC 
CTATCCATAA ATGCCACAAA CATCGACTCC TTCAAAAACT GTACGAAGAT CAATGGGGAT 
GTCAGCATTC TTCCTGTTGC ATTTCTAGGG GATGCCTTCA CAAAGACACT ACCCCTTGAC 
CCTAAGAAGC TGGATGTCTT TAGAACAGTC AAAGAAATAT CAGGATTTTT GTTGATTCAG 
GCCTGGCCTG ATAATGCTAC TGATCTCTAT GCTTTTGAAA ATCTGGAGAT TATCCGAGGC 
CGAACCAAGC AGCACGGCCA GTATTCCCTT GCTGTTGTTA ACTTGAAAAT ACAGTCGTTG 
GGGCTGCGCT CCCTCAAGGA AATAAGTGAT GGAGACATTG CCATTATGAA GAACAAGAAC 
CTCTGCTATG CTGACACCAT GAACTGGCGC AGCTTGTTTG CTACTCAGAG TCAGAAAACA 
AAAATTATAC AGAACAGAAA TAAAAATGAT TGTACTGCTG ACAGGCATGT GTGTGACCCG 
CTGTGCTCGG ACGTGGGCTG CTGGGGCCCA GGGCCCTTCC ACTGCTTTTC CTGCAGGTTT 
TTCAGTCGCC AGAAGGAGTG TGTAAAACAG TGCAACATCC TGCAAGGGGA GCCACGTGAG 
TTTGAAAGAG ACTCCAAATG CCTCCCCTGC CACTCAGAGT GTCTGGTACA GAACTCCACT 
GCATACAACA CAACCTGCTC TGGACCGGGC CCAGACCACT GCATGAAGTG TGCCCATTTT 
ATAGATGGTC CCCACTGTGT GAAGGCCTGC CCCGCTGGGG TCCTGGGTGA GAATGATACC 
CTGGTCTGGA AGTATGCAGA TGCCAATGCT GTTTGCCAGC TCTGCCATCC AAACTGTACA 
CGAGGGTGCA AAGGACCAGG TCTTGAAGGA TGTCCAAATG GCTCCAAAAC TCCATCTATC 
GCGGCTGGTG TTGTCGGAGG ACTCCTGTGC CTGGTTGTGG TTGGTCTAGG CATCGGTCTT 
TACCTGCGGC GACGTCATAT CGTGCGGAAG CGCACCCTGC GCAGGCTGCT GCAAGAGAGG 
GAGCTTGTCG AACCACTGAC ACCCAGTGGG GAGGCACCAA ACCAGGCCCA CCTGAGAATT 
TTAAAGGAAA CAGAATTTAA AAAGGTCAAA GTTTTGGGCT CTGGAGCTTT TGGCACTGTT 
TATAAGGGAC TTTGGATCCC AGAAGGGGAA AAGGTTAAAA TTCCTGTTGC TATTAAAGAA 
TTGAGAGAGG CTACATCGCC AAAAGCCAAC AAGGAAATAC TTGATGAAGC CTATGTGATG 
GCTAGTGTTG ACAATCCTCA TGTGTGCCGC TTGCTGGGAA TCTGCCTCAC TTCCACTGTG 
CAGCTCATCA CCCAGCTTAT GCCTTATGGC TGCCTCCTTG ACTACATCCG AGAGCACAAG 
GACAACATTG GCTCCCAGTA CCTTCTCAAC TGGTGTGTGC AGATTGCAAA GGGAATGAAC 
TACCTGGAGG AACGTCGCCT GGTGCACCGT GACCTTGCTG CCAGGAACGT CCTTGTTAAG 
ACTCCACAAC ATGTGAAAAT CACAGACTTT GGGCTGGCAA AGCTGCTTGG GGCAGATGAG 
AAGGAGTATC ACGCAGAGGG AGGCAAGGTT CCTATTAAAT GGATGGCATT GGAGTCAATT 
TTACACCGAA TTTATACTCA TCAAAGTGAT GTCTGGAGTT ATGGTGTGAC AGTTTGGGAG 
TTGATGACAT TTGGGTCCAA GCCTTATGAC GGGATCCCCG CAAGTGAAAT CTCCTCCGTC 
TTGGAGAAGG GAGAGCGTTT GCCCCAGCCA CCCATTTGTA CCATTGATGT GTACATGATC 
ATGGTCAAAT GCTGGATGAT TGATGCAGAC AGCCGTCCCA AGTTTCGTGA GCTGATTGCA 
GAGTTCTCCA AAATGGCTCG TGACCCTCCC CGCTATCTTG TTATACAGGG AGATGAAAGG 
ATGCACTTGC CTAGCCCTAC AGATTCCAAG TTTTATCGCA CCCTGATGGA GGAGGAGGAC 
ATGGAAGACA TTGTGGATGC AGATGAGTAT CTTGTCCCAC ACCAGGGCTT TTTCAACAGC 
CCCTCTACAT CTCGGACTCC TCTTCTGAGT TCATTGAGCG CTACTAGCAA CAATTCTGCT 
ACAAACTGCA TTGACAGAAA TGGGCAGGGG CACCCTGTGA GGGAAGACAG CTTTGTCCAG 
AGGTACAGCT CAGATCCAAC AGGAAACTTC TTGGAGGAGA GTATAGATGA TGGCTTCCTG 
CCTGCTCCAG AGTATGTAAA CCAGCTGATG CCCAAGAAAC CATCTACTGC CATGGTCCAG 
AATCAAATCT ACAACAACAT CTCTCTCACA GCAATCTCAA AGCTCCCCAT GGACTCAAGA 
TACCAGAATT CCCACAGCAC AGCTGTGGAC AACCCTGAGT ATCTCAACAC TAACCAGTCC 
CCACTGGCCA AAACAGTCTT CGAGAGCTCT CCCTATTGGA TCCAATCAGG CAATCACCAA 
ATAAATCTGG ACAATCCTGA CTACCAGCAG GACTTTTTAC CAAATGAAAC AAAACCTAAT 
GGTCTCTTGA AAGTTCCTGC AGCAGAAAAC CCAGAGTACT TGAGGGTAGC AGCACCAAAA 
AGTGAATACA TTGAAGCTTC AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025002843.1
CDS	396..4142
Translation	MGVRSPLSASGPRGAAVLVLLLLLLGRVALCSAVEEKKVCQGTNNKLTQLGHVEDHFTSLQRMYNNCEVVLSNLEITYVEHNRDLTFLKTIQEVAGYVLIALNMVDVIPLENLQIIRGNVLYDNSFALAVLSNYHMNKTQGLRELPMKRLSEILNGGVKISNNPKLCNMDTVLWNDIIDTSRKPLTVLDFASNLSSSCTHCPLSYHWMSLRGAWILEPCPKCHPNCTEDHCWGAGEQNCQTLTKVICAQQCSGRCRGKVPSDCCHNQCAAGCTGPRESDCLACRKFRDDATCKDTCPPLVLYNPTTYQMDVNPEGKYSFGATCVRECPHNYVVTDHGSCVRSCNTDTYEVEENGVRKCKKCDGLCSKVCNGIGIGELKGILSINATNIDSFKNCTKINGDVSILPVAFLGDAFTKTLPLDPKKLDVFRTVKEISGFLLIQAWPDNATDLYAFENLEIIRGRTKQHGQYSLAVVNLKIQSLGLRSLKEISDGDIAIMKNKNLCYADTMNWRSLFATQSQKTKIIQNRNKNDCTADRHVCDPLCSDVGCWGPGPFHCFSCRFFSRQKECVKQCNILQGEPREFERDSKCLPCHSECLVQNSTAYNTTCSGPGPDHCMKCAHFIDGPHCVKACPAGVLGENDTLVWKYADANAVCQLCHPNCTRGCKGPGLEGCPNGSKTPSIAAGVVGGLLCLVVVGLGIGLYLRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQAHLRILKETEFKKVKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPYGCLLDYIREHKDNIGSQYLLNWCVQIAKGMNYLEERRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGADEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSVLEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIAEFSKMARDPPRYLVIQGDERMHLPSPTDSKFYRTLMEEEDMEDIVDADEYLVPHQGFFNSPSTSRTPLLSSLSATSNNSATNCIDRNGQGHPVREDSFVQRYSSDPTGNFLEESIDDGFLPAPEYVNQLMPKKPSTAMVQNQIYNNISLTAISKLPMDSRYQNSHSTAVDNPEYLNTNQSPLAKTVFESSPYWIQSGNHQINLDNPDYQQDFLPNETKPNGLLKVPAAENPEYLRVAAPKSEYIEASA

Target ORF information:

RefSeq Version	XM_025147075.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus epidermal growth factor receptor (EGFR), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025147075.1

ORF Insert Sequence:

ATGGGTGTAC GCAGCCCCCT GTCCGCCTCT GGGCCTCGCG GGGCCGCTGT CCTGGTGCTA 
CTGCTGCTGC TGCTGGGGCG CGTCGCGCTG TGTTCCGCCG TGGAGGAGAA GAAAGTTTGT 
CAAGGGACAA ATAACAAGTT GACCCAGCTG GGGCACGTGG AAGACCATTT CACCAGCCTG 
CAGAGAATGT ACAACAACTG CGAAGTGGTA CTGAGTAACC TGGAGATTAC CTACGTGGAG 
CATAATCGCG ATCTCACCTT CCTTAAGACC ATACAGGAGG TTGCAGGCTA TGTGCTCATT 
GCGCTTAACA TGGTGGACGT CATTCCCTTA GAAAACCTCC AGATTATCCG AGGGAATGTG 
CTTTATGACA ACTCTTTTGC CCTGGCAGTT TTATCCAATT ACCACATGAA TAAAACCCAG 
GGACTTCGAG AGCTGCCAAT GAAACGGCTA TCAGAAATTC TCAATGGAGG TGTTAAAATC 
AGCAACAACC CCAAACTGTG CAACATGGAC ACTGTTCTCT GGAATGACAT CATTGATACA 
AGCAGGAAGC CTCTCACAGT ACTTGACTTT GCAAGCAATC TTTCTTCTTC TTGCACCCAT 
TGCCCCTTGT CCTATCACTG GATGTCACTG AGAGGAGCCT GGATCCTTGA GCCTTGTCCA 
AAATGCCATC CGAACTGCAC AGAAGACCAC TGCTGGGGTG CTGGTGAACA GAACTGCCAG 
ACTTTAACAA AAGTCATCTG TGCCCAGCAA TGCTCTGGCC GGTGCAGAGG AAAGGTGCCC 
AGTGACTGCT GCCACAATCA GTGTGCTGCA GGGTGCACAG GACCTCGGGA GAGTGACTGC 
CTGGCATGCC GCAAGTTTCG GGATGATGCT ACCTGCAAGG ACACATGTCC CCCACTGGTC 
CTCTATAACC CCACCACCTA TCAAATGGAT GTCAACCCCG AGGGAAAATA CAGCTTTGGA 
GCCACTTGTG TGAGGGAATG TCCACACAAC TATGTGGTGA CAGATCATGG CTCCTGCGTT 
CGCTCGTGTA ATACTGATAC TTACGAAGTG GAAGAAAATG GTGTTCGGAA GTGTAAAAAA 
TGTGATGGGC TATGTAGCAA AGTGTGCAAT GGCATTGGAA TAGGTGAACT TAAAGGGATC 
CTATCCATAA ATGCCACAAA CATCGACTCC TTCAAAAACT GTACGAAGAT CAATGGGGAT 
GTCAGCATTC TTCCTGTTGC ATTTCTAGGG GATGCCTTCA CAAAGACACT ACCCCTTGAC 
CCTAAGAAGC TGGATGTCTT TAGAACAGTC AAAGAAATAT CAGGATTTTT GTTGATTCAG 
GCCTGGCCTG ATAATGCTAC TGATCTCTAT GCTTTTGAAA ATCTGGAGAT TATCCGAGGC 
CGAACCAAGC AGCACGGCCA GTATTCCCTT GCTGTTGTTA ACTTGAAAAT ACAGTCGTTG 
GGGCTGCGCT CCCTCAAGGA AATAAGTGAT GGAGACATTG CCATTATGAA GAACAAGAAC 
CTCTGCTATG CTGACACCAT GAACTGGCGC AGCTTGTTTG CTACTCAGAG TCAGAAAACA 
AAAATTATAC AGAACAGAAA TAAAAATGAT TGTACTGCTG ACAGGCATGT GTGTGACCCG 
CTGTGCTCGG ACGTGGGCTG CTGGGGCCCA GGGCCCTTCC ACTGCTTTTC CTGCAGGTTT 
TTCAGTCGCC AGAAGGAGTG TGTAAAACAG TGCAACATCC TGCAAGGGGA GCCACGTGAG 
TTTGAAAGAG ACTCCAAATG CCTCCCCTGC CACTCAGAGT GTCTGGTACA GAACTCCACT 
GCATACAACA CAACCTGCTC TGGACCGGGC CCAGACCACT GCATGAAGTG TGCCCATTTT 
ATAGATGGTC CCCACTGTGT GAAGGCCTGC CCCGCTGGGG TCCTGGGTGA GAATGATACC 
CTGGTCTGGA AGTATGCAGA TGCCAATGCT GTTTGCCAGC TCTGCCATCC AAACTGTACA 
CGAGGGTGCA AAGGACCAGG TCTTGAAGGA TGTCCAAATG GCTCCAAAAC TCCATCTATC 
GCGGCTGGTG TTGTCGGAGG ACTCCTGTGC CTGGTTGTGG TTGGTCTAGG CATCGGTCTT 
TACCTGCGGC GACGTCATAT CGTGCGGAAG CGCACCCTGC GCAGGCTGCT GCAAGAGAGG 
GAGCTTGTCG AACCACTGAC ACCCAGTGGG GAGGCACCAA ACCAGGCCCA CCTGAGAATT 
TTAAAGGAAA CAGAATTTAA AAAGGTCAAA GTTTTGGGCT CTGGAGCTTT TGGCACTGTT 
TATAAGGGAC TTTGGATCCC AGAAGGGGAA AAGGTTAAAA TTCCTGTTGC TATTAAAGAA 
TTGAGAGAGG CTACATCGCC AAAAGCCAAC AAGGAAATAC TTGATGAAGC CTATGTGATG 
GCTAGTGTTG ACAATCCTCA TGTGTGCCGC TTGCTGGGAA TCTGCCTCAC TTCCACTGTG 
CAGCTCATCA CCCAGCTTAT GCCTTATGGC TGCCTCCTTG ACTACATCCG AGAGCACAAG 
GACAACATTG GCTCCCAGTA CCTTCTCAAC TGGTGTGTGC AGATTGCAAA GGGAATGAAC 
TACCTGGAGG AACGTCGCCT GGTGCACCGT GACCTTGCTG CCAGGAACGT CCTTGTTAAG 
ACTCCACAAC ATGTGAAAAT CACAGACTTT GGGCTGGCAA AGCTGCTTGG GGCAGATGAG 
AAGGAGTATC ACGCAGAGGG AGGCAAGGTT CCTATTAAAT GGATGGCATT GGAGTCAATT 
TTACACCGAA TTTATACTCA TCAAAGTGAT GTCTGGAGTT ATGGTGTGAC AGTTTGGGAG 
TTGATGACAT TTGGGTCCAA GCCTTATGAC GGGATCCCCG CAAGTGAAAT CTCCTCCGTC 
TTGGAGAAGG GAGAGCGTTT GCCCCAGCCA CCCATTTGTA CCATTGATGT GTACATGATC 
ATGGTCAAAT GCTGGATGAT TGATGCAGAC AGCCGTCCCA AGTTTCGTGA GCTGATTGCA 
GAGTTCTCCA AAATGGCTCG TGACCCTCCC CGCTATCTTG TTATACAGGG AGATGAAAGG 
ATGCACTTGC CTAGCCCTAC AGATTCCAAG TTTTATCGCA CCCTGATGGA GGAGGAGGAC 
ATGGAAGACA TTGTGGATGC AGATGAGTAT CTTGTCCCAC ACCAGGGCTT TTTCAACAGC 
CCCTCTACAT CTCGGACTCC TCTTCTGAGT TCATTGAGCG CTACTAGCAA CAATTCTGCT 
ACAAACTGCA TTGACAGAAA TGGGCAGGGG CACCCTGTGA GGGAAGACAG CTTTGTCCAG 
AGGTACAGCT CAGATCCAAC AGGAAACTTC TTGGAGGAGA GTATAGATGA TGGCTTCCTG 
CCTGCTCCAG AGTATGTAAA CCAGCTGATG CCCAAGAAAC CATCTACTGC CATGGTCCAG 
AATCAAATCT ACAACAACAT CTCTCTCACA GCAATCTCAA AGCTCCCCAT GGACTCAAGA 
TACCAGAATT CCCACAGCAC AGCTGTGGAC AACCCTGAGT ATCTCAACAC TAACCAGTCC 
CCACTGGCCA AAACAGTCTT CGAGAGCTCT CCCTATTGGA TCCAATCAGG CAATCACCAA 
ATAAATCTGG ACAATCCTGA CTACCAGCAG GACTTTTTAC CAAATGAAAC AAAACCTAAT 
GGTCTCTTGA AAGTTCCTGC AGCAGAAAAC CCAGAGTACT TGAGGGTAGC AGCACCAAAA 
AGTGAATACA TTGAAGCTTC AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGFR ( XM_025147076.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025002844.1 Gallus gallus epidermal growth factor receptor (EGFR), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa145766
Clone ID Related Accession (Same CDS sequence)	XM_025147076.1
Accession Version	XM_025147076.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	epidermal growth factor receptor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGTAC GCAGCCCCCT GTCCGCCTCT GGGCCTCGCG GGGCCGCTGT CCTGGTGCTA 
CTGCTGCTGC TGCTGGGGCG CGTCGCGCTG TGTTCCGCCG TGGAGGAGAA GAAAGTTTGT 
CAAGGGACAA ATAACAAGTT GACCCAGCTG GGGCACGTGG AAGACCATTT CACCAGCCTG 
CAGAGAATGT ACAACAACTG CGAAGTGGTA CTGAGTAACC TGGAGATTAC CTACGTGGAG 
CATAATCGCG ATCTCACCTT CCTTAAGACC ATACAGGAGG TTGCAGGCTA TGTGCTCATT 
GCGCTTAACA TGGTGGACGT CATTCCCTTA GAAAACCTCC AGATTATCCG AGGGAATGTG 
CTTTATGACA ACTCTTTTGC CCTGGCAGTT TTATCCAATT ACCACATGAA TAAAACCCAG 
GGACTTCGAG AGCTGCCAAT GAAACGGCTA TCAGAAATTC TCAATGGAGG TGTTAAAATC 
AGCAACAACC CCAAACTGTG CAACATGGAC ACTGTTCTCT GGAATGACAT CATTGATACA 
AGCAGGAAGC CTCTCACAGT ACTTGACTTT GCAAGCAATC TTTCTTCTTC TTGCACCCAT 
TGCCCCTTGT CCTATCACTG GATGTCACTG AGAGGAGCCT GGATCCTTGA GCCTTGTCCA 
AAATGCCATC CGAACTGCAC AGAAGACCAC TGCTGGGGTG CTGGTGAACA GAACTGCCAG 
ACTTTAACAA AAGTCATCTG TGCCCAGCAA TGCTCTGGCC GGTGCAGAGG AAAGGTGCCC 
AGTGACTGCT GCCACAATCA GTGTGCTGCA GGGTGCACAG GACCTCGGGA GAGTGACTGC 
CTGGCATGCC GCAAGTTTCG GGATGATGCT ACCTGCAAGG ACACATGTCC CCCACTGGTC 
CTCTATAACC CCACCACCTA TCAAATGGAT GTCAACCCCG AGGGAAAATA CAGCTTTGGA 
GCCACTTGTG TGAGGGAATG TCCACACAAC TATGTGGTGA CAGATCATGG CTCCTGCGTT 
CGCTCGTGTA ATACTGATAC TTACGAAGTG GAAGAAAATG GTGTTCGGAA GTGTAAAAAA 
TGTGATGGGC TATGTAGCAA AGTGTGCAAT GGCATTGGAA TAGGTGAACT TAAAGGGATC 
CTATCCATAA ATGCCACAAA CATCGACTCC TTCAAAAACT GTACGAAGAT CAATGGGGAT 
GTCAGCATTC TTCCTGTTGC ATTTCTAGGG GATGCCTTCA CAAAGACACT ACCCCTTGAC 
CCTAAGAAGC TGGATGTCTT TAGAACAGTC AAAGAAATAT CAGGATTTTT GTTGATTCAG 
GCCTGGCCTG ATAATGCTAC TGATCTCTAT GCTTTTGAAA ATCTGGAGAT TATCCGAGGC 
CGAACCAAGC AGCACGGCCA GTATTCCCTT GCTGTTGTTA ACTTGAAAAT ACAGTCGTTG 
GGGCTGCGCT CCCTCAAGGA AATAAGTGAT GGAGACATTG CCATTATGAA GAACAAGAAC 
CTCTGCTATG CTGACACCAT GAACTGGCGC AGCTTGTTTG CTACTCAGAG TCAGAAAACA 
AAAATTATAC AGAACAGAAA TAAAAATGAT TGTACTGCTG ACAGGCATGT GTGTGACCCG 
CTGTGCTCGG ACGTGGGCTG CTGGGGCCCA GGGCCCTTCC ACTGCTTTTC CTGCAGGTTT 
TTCAGTCGCC AGAAGGAGTG TGTAAAACAG TGCAACATCC TGCAAGGGGA GCCACGTGAG 
TTTGAAAGAG ACTCCAAATG CCTCCCCTGC CACTCAGAGT GTCTGGTACA GAACTCCACT 
GCATACAACA CAACCTGCTC TGGACCGGGC CCAGACCACT GCATGAAGTG TGCCCATTTT 
ATAGATGGTC CCCACTGTGT GAAGGCCTGC CCCGCTGGGG TCCTGGGTGA GAATGATACC 
CTGGTCTGGA AGTATGCAGA TGCCAATGCT GTTTGCCAGC TCTGCCATCC AAACTGTACA 
CGAGGGTGCA AAGGACCAGG TCTTGAAGGA TGTCCAAATG GCTCCAAAAC TCCATCTATC 
GCGGCTGGTG TTGTCGGAGG ACTCCTGTGC CTGGTTGTGG TTGGTCTAGG CATCGGTCTT 
TACCTGCGGC GACGTCATAT CGTGCGGAAG CGCACCCTGC GCAGGCTGCT GCAAGAGAGG 
GAGCTTGTCG AACCACTGAC ACCCAGTGGG GAGGCACCAA ACCAGGCCCA CCTGAGAATT 
TTAAAGGAAA CAGAATTTAA AAAGGTCAAA GTTTTGGGCT CTGGAGCTTT TGGCACTGTT 
TATAAGGGAC TTTGGATCCC AGAAGGGGAA AAGGTTAAAA TTCCTGTTGC TATTAAAGAA 
TTGAGAGAGG CTACATCGCC AAAAGCCAAC AAGGAAATAC TTGATGAAGC CTATGTGATG 
GCTAGTGTTG ACAATCCTCA TGTGTGCCGC TTGCTGGGAA TCTGCCTCAC TTCCACTGTG 
CAGCTCATCA CCCAGCTTAT GCCTTATGGC TGCCTCCTTG ACTACATCCG AGAGCACAAG 
GACAACATTG GCTCCCAGTA CCTTCTCAAC TGGTGTGTGC AGATTGCAAA GGGAATGAAC 
TACCTGGAGG AACGTCGCCT GGTGCACCGT GACCTTGCTG CCAGGAACGT CCTTGTTAAG 
ACTCCACAAC ATGTGAAAAT CACAGACTTT GGGCTGGCAA AGCTGCTTGG GGCAGATGAG 
AAGGAGTATC ACGCAGAGGG AGGCAAGGTT CCTATTAAAT GGATGGCATT GGAGTCAATT 
TTACACCGAA TTTATACTCA TCAAAGTGAT GTCTGGAGTT ATGGTGTGAC AGTTTGGGAG 
TTGATGACAT TTGGGTCCAA GCCTTATGAC GGGATCCCCG CAAGTGAAAT CTCCTCCGTC 
TTGGAGAAGG GAGAGCGTTT GCCCCAGCCA CCCATTTGTA CCATTGATGT GTACATGATC 
ATGGTCAAAT GCTGGATGAT TGATGCAGAC AGCCGTCCCA AGTTTCGTGA GCTGATTGCA 
GAGTTCTCCA AAATGGCTCG TGACCCTCCC CGCTATCTTG TTATACAGGG AGATGAAAGG 
ATGCACTTGC CTAGCCCTAC AGATTCCAAG TTTTATCGCA CCCTGATGGA GGAGGAGGAC 
ATGGAAGACA TTGTGGATGC AGATGAGTAT CTTGTCCCAC ACCAGGGCTT TTTCAACAGC 
CCCTCTACAT CTCGGACTCC TCTTCTGAGT TCATTGAGCG CTACTAGCAA CAATTCTGCT 
ACAAACTGCA TTGACAGAAA TGGGGGGCAC CCTGTGAGGG AAGACAGCTT TGTCCAGAGG 
TACAGCTCAG ATCCAACAGG AAACTTCTTG GAGGAGAGTA TAGATGATGG CTTCCTGCCT 
GCTCCAGAGT ATGTAAACCA GCTGATGCCC AAGAAACCAT CTACTGCCAT GGTCCAGAAT 
CAAATCTACA ACAACATCTC TCTCACAGCA ATCTCAAAGC TCCCCATGGA CTCAAGATAC 
CAGAATTCCC ACAGCACAGC TGTGGACAAC CCTGAGTATC TCAACACTAA CCAGTCCCCA 
CTGGCCAAAA CAGTCTTCGA GAGCTCTCCC TATTGGATCC AATCAGGCAA TCACCAAATA 
AATCTGGACA ATCCTGACTA CCAGCAGGAC TTTTTACCAA ATGAAACAAA ACCTAATGGT 
CTCTTGAAAG TTCCTGCAGC AGAAAACCCA GAGTACTTGA GGGTAGCAGC ACCAAAAAGT 
GAATACATTG AAGCTTCAGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025002844.1
CDS	400..4143
Translation	MGVRSPLSASGPRGAAVLVLLLLLLGRVALCSAVEEKKVCQGTNNKLTQLGHVEDHFTSLQRMYNNCEVVLSNLEITYVEHNRDLTFLKTIQEVAGYVLIALNMVDVIPLENLQIIRGNVLYDNSFALAVLSNYHMNKTQGLRELPMKRLSEILNGGVKISNNPKLCNMDTVLWNDIIDTSRKPLTVLDFASNLSSSCTHCPLSYHWMSLRGAWILEPCPKCHPNCTEDHCWGAGEQNCQTLTKVICAQQCSGRCRGKVPSDCCHNQCAAGCTGPRESDCLACRKFRDDATCKDTCPPLVLYNPTTYQMDVNPEGKYSFGATCVRECPHNYVVTDHGSCVRSCNTDTYEVEENGVRKCKKCDGLCSKVCNGIGIGELKGILSINATNIDSFKNCTKINGDVSILPVAFLGDAFTKTLPLDPKKLDVFRTVKEISGFLLIQAWPDNATDLYAFENLEIIRGRTKQHGQYSLAVVNLKIQSLGLRSLKEISDGDIAIMKNKNLCYADTMNWRSLFATQSQKTKIIQNRNKNDCTADRHVCDPLCSDVGCWGPGPFHCFSCRFFSRQKECVKQCNILQGEPREFERDSKCLPCHSECLVQNSTAYNTTCSGPGPDHCMKCAHFIDGPHCVKACPAGVLGENDTLVWKYADANAVCQLCHPNCTRGCKGPGLEGCPNGSKTPSIAAGVVGGLLCLVVVGLGIGLYLRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQAHLRILKETEFKKVKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPYGCLLDYIREHKDNIGSQYLLNWCVQIAKGMNYLEERRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGADEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSVLEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIAEFSKMARDPPRYLVIQGDERMHLPSPTDSKFYRTLMEEEDMEDIVDADEYLVPHQGFFNSPSTSRTPLLSSLSATSNNSATNCIDRNGGHPVREDSFVQRYSSDPTGNFLEESIDDGFLPAPEYVNQLMPKKPSTAMVQNQIYNNISLTAISKLPMDSRYQNSHSTAVDNPEYLNTNQSPLAKTVFESSPYWIQSGNHQINLDNPDYQQDFLPNETKPNGLLKVPAAENPEYLRVAAPKSEYIEASA

Target ORF information:

RefSeq Version	XM_025147076.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus epidermal growth factor receptor (EGFR), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025147076.1

ORF Insert Sequence:

ATGGGTGTAC GCAGCCCCCT GTCCGCCTCT GGGCCTCGCG GGGCCGCTGT CCTGGTGCTA 
CTGCTGCTGC TGCTGGGGCG CGTCGCGCTG TGTTCCGCCG TGGAGGAGAA GAAAGTTTGT 
CAAGGGACAA ATAACAAGTT GACCCAGCTG GGGCACGTGG AAGACCATTT CACCAGCCTG 
CAGAGAATGT ACAACAACTG CGAAGTGGTA CTGAGTAACC TGGAGATTAC CTACGTGGAG 
CATAATCGCG ATCTCACCTT CCTTAAGACC ATACAGGAGG TTGCAGGCTA TGTGCTCATT 
GCGCTTAACA TGGTGGACGT CATTCCCTTA GAAAACCTCC AGATTATCCG AGGGAATGTG 
CTTTATGACA ACTCTTTTGC CCTGGCAGTT TTATCCAATT ACCACATGAA TAAAACCCAG 
GGACTTCGAG AGCTGCCAAT GAAACGGCTA TCAGAAATTC TCAATGGAGG TGTTAAAATC 
AGCAACAACC CCAAACTGTG CAACATGGAC ACTGTTCTCT GGAATGACAT CATTGATACA 
AGCAGGAAGC CTCTCACAGT ACTTGACTTT GCAAGCAATC TTTCTTCTTC TTGCACCCAT 
TGCCCCTTGT CCTATCACTG GATGTCACTG AGAGGAGCCT GGATCCTTGA GCCTTGTCCA 
AAATGCCATC CGAACTGCAC AGAAGACCAC TGCTGGGGTG CTGGTGAACA GAACTGCCAG 
ACTTTAACAA AAGTCATCTG TGCCCAGCAA TGCTCTGGCC GGTGCAGAGG AAAGGTGCCC 
AGTGACTGCT GCCACAATCA GTGTGCTGCA GGGTGCACAG GACCTCGGGA GAGTGACTGC 
CTGGCATGCC GCAAGTTTCG GGATGATGCT ACCTGCAAGG ACACATGTCC CCCACTGGTC 
CTCTATAACC CCACCACCTA TCAAATGGAT GTCAACCCCG AGGGAAAATA CAGCTTTGGA 
GCCACTTGTG TGAGGGAATG TCCACACAAC TATGTGGTGA CAGATCATGG CTCCTGCGTT 
CGCTCGTGTA ATACTGATAC TTACGAAGTG GAAGAAAATG GTGTTCGGAA GTGTAAAAAA 
TGTGATGGGC TATGTAGCAA AGTGTGCAAT GGCATTGGAA TAGGTGAACT TAAAGGGATC 
CTATCCATAA ATGCCACAAA CATCGACTCC TTCAAAAACT GTACGAAGAT CAATGGGGAT 
GTCAGCATTC TTCCTGTTGC ATTTCTAGGG GATGCCTTCA CAAAGACACT ACCCCTTGAC 
CCTAAGAAGC TGGATGTCTT TAGAACAGTC AAAGAAATAT CAGGATTTTT GTTGATTCAG 
GCCTGGCCTG ATAATGCTAC TGATCTCTAT GCTTTTGAAA ATCTGGAGAT TATCCGAGGC 
CGAACCAAGC AGCACGGCCA GTATTCCCTT GCTGTTGTTA ACTTGAAAAT ACAGTCGTTG 
GGGCTGCGCT CCCTCAAGGA AATAAGTGAT GGAGACATTG CCATTATGAA GAACAAGAAC 
CTCTGCTATG CTGACACCAT GAACTGGCGC AGCTTGTTTG CTACTCAGAG TCAGAAAACA 
AAAATTATAC AGAACAGAAA TAAAAATGAT TGTACTGCTG ACAGGCATGT GTGTGACCCG 
CTGTGCTCGG ACGTGGGCTG CTGGGGCCCA GGGCCCTTCC ACTGCTTTTC CTGCAGGTTT 
TTCAGTCGCC AGAAGGAGTG TGTAAAACAG TGCAACATCC TGCAAGGGGA GCCACGTGAG 
TTTGAAAGAG ACTCCAAATG CCTCCCCTGC CACTCAGAGT GTCTGGTACA GAACTCCACT 
GCATACAACA CAACCTGCTC TGGACCGGGC CCAGACCACT GCATGAAGTG TGCCCATTTT 
ATAGATGGTC CCCACTGTGT GAAGGCCTGC CCCGCTGGGG TCCTGGGTGA GAATGATACC 
CTGGTCTGGA AGTATGCAGA TGCCAATGCT GTTTGCCAGC TCTGCCATCC AAACTGTACA 
CGAGGGTGCA AAGGACCAGG TCTTGAAGGA TGTCCAAATG GCTCCAAAAC TCCATCTATC 
GCGGCTGGTG TTGTCGGAGG ACTCCTGTGC CTGGTTGTGG TTGGTCTAGG CATCGGTCTT 
TACCTGCGGC GACGTCATAT CGTGCGGAAG CGCACCCTGC GCAGGCTGCT GCAAGAGAGG 
GAGCTTGTCG AACCACTGAC ACCCAGTGGG GAGGCACCAA ACCAGGCCCA CCTGAGAATT 
TTAAAGGAAA CAGAATTTAA AAAGGTCAAA GTTTTGGGCT CTGGAGCTTT TGGCACTGTT 
TATAAGGGAC TTTGGATCCC AGAAGGGGAA AAGGTTAAAA TTCCTGTTGC TATTAAAGAA 
TTGAGAGAGG CTACATCGCC AAAAGCCAAC AAGGAAATAC TTGATGAAGC CTATGTGATG 
GCTAGTGTTG ACAATCCTCA TGTGTGCCGC TTGCTGGGAA TCTGCCTCAC TTCCACTGTG 
CAGCTCATCA CCCAGCTTAT GCCTTATGGC TGCCTCCTTG ACTACATCCG AGAGCACAAG 
GACAACATTG GCTCCCAGTA CCTTCTCAAC TGGTGTGTGC AGATTGCAAA GGGAATGAAC 
TACCTGGAGG AACGTCGCCT GGTGCACCGT GACCTTGCTG CCAGGAACGT CCTTGTTAAG 
ACTCCACAAC ATGTGAAAAT CACAGACTTT GGGCTGGCAA AGCTGCTTGG GGCAGATGAG 
AAGGAGTATC ACGCAGAGGG AGGCAAGGTT CCTATTAAAT GGATGGCATT GGAGTCAATT 
TTACACCGAA TTTATACTCA TCAAAGTGAT GTCTGGAGTT ATGGTGTGAC AGTTTGGGAG 
TTGATGACAT TTGGGTCCAA GCCTTATGAC GGGATCCCCG CAAGTGAAAT CTCCTCCGTC 
TTGGAGAAGG GAGAGCGTTT GCCCCAGCCA CCCATTTGTA CCATTGATGT GTACATGATC 
ATGGTCAAAT GCTGGATGAT TGATGCAGAC AGCCGTCCCA AGTTTCGTGA GCTGATTGCA 
GAGTTCTCCA AAATGGCTCG TGACCCTCCC CGCTATCTTG TTATACAGGG AGATGAAAGG 
ATGCACTTGC CTAGCCCTAC AGATTCCAAG TTTTATCGCA CCCTGATGGA GGAGGAGGAC 
ATGGAAGACA TTGTGGATGC AGATGAGTAT CTTGTCCCAC ACCAGGGCTT TTTCAACAGC 
CCCTCTACAT CTCGGACTCC TCTTCTGAGT TCATTGAGCG CTACTAGCAA CAATTCTGCT 
ACAAACTGCA TTGACAGAAA TGGGGGGCAC CCTGTGAGGG AAGACAGCTT TGTCCAGAGG 
TACAGCTCAG ATCCAACAGG AAACTTCTTG GAGGAGAGTA TAGATGATGG CTTCCTGCCT 
GCTCCAGAGT ATGTAAACCA GCTGATGCCC AAGAAACCAT CTACTGCCAT GGTCCAGAAT 
CAAATCTACA ACAACATCTC TCTCACAGCA ATCTCAAAGC TCCCCATGGA CTCAAGATAC 
CAGAATTCCC ACAGCACAGC TGTGGACAAC CCTGAGTATC TCAACACTAA CCAGTCCCCA 
CTGGCCAAAA CAGTCTTCGA GAGCTCTCCC TATTGGATCC AATCAGGCAA TCACCAAATA 
AATCTGGACA ATCCTGACTA CCAGCAGGAC TTTTTACCAA ATGAAACAAA ACCTAATGGT 
CTCTTGAAAG TTCCTGCAGC AGAAAACCCA GAGTACTTGA GGGTAGCAGC ACCAAAAAGT 
GAATACATTG AAGCTTCAGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGFR ( XM_015281328.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015136814.1 Gallus gallus epidermal growth factor receptor (EGFR), transcript variant X3, mRNA.

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CloneID	OGa31512
Clone ID Related Accession (Same CDS sequence)	XM_015281328.2 , XM_015281328.1
Accession Version	XM_015281328.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	epidermal growth factor receptor isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015281328.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGTAC GCAGCCCCCT GTCCGCCTCT GGGCCTCGCG GGGCCGCTGT CCTGGTGCTA 
CTGCTGCTGC TGCTGGGGCG CGTCGCGCTG TGTTCCGCCG TGGAGGAGAA GAAAGTTTGT 
CAAGGGACAA ATAACAAGTT GACCCAGCTG GGGCACGTGG AAGACCATTT CACCAGCCTG 
CAGAGAATGT ACAACAACTG CGAAGTGGTA CTGAGTAACC TGGAGATTAC CTACGTGGAG 
CATAATCGCG ATCTCACCTT CCTTAAGACC ATACAGGAGG TTGCAGGCTA TGTGCTCATT 
GCGCTTAACA TGGTGGACGT CATTCCCTTA GAAAACCTCC AGATTATCCG AGGGAATGTG 
CTTTATGACA ACTCTTTTGC CCTGGCAGTT TTATCCAATT ACCACATGAA TAAAACCCAG 
GGACTTCGAG AGCTGCCAAT GAAACGGCTA TCAGAAATTC TCAATGGAGG TGTTAAAATC 
AGCAACAACC CCAAACTGTG CAACATGGAC ACTGTTCTCT GGAATGACAT CATTGATACA 
AGCAGGAAGC CTCTCACAGT ACTTGACTTT GCAAGCAATC TTTCTTCTTG TCCAAAATGC 
CATCCGAACT GCACAGAAGA CCACTGCTGG GGTGCTGGTG AACAGAACTG CCAGACTTTA 
ACAAAAGTCA TCTGTGCCCA GCAATGCTCT GGCCGGTGCA GAGGAAAGGT GCCCAGTGAC 
TGCTGCCACA ATCAGTGTGC TGCAGGGTGC ACAGGACCTC GGGAGAGTGA CTGCCTGGCA 
TGCCGCAAGT TTCGGGATGA TGCTACCTGC AAGGACACAT GTCCCCCACT GGTCCTCTAT 
AACCCCACCA CCTATCAAAT GGATGTCAAC CCCGAGGGAA AATACAGCTT TGGAGCCACT 
TGTGTGAGGG AATGTCCACA CAACTATGTG GTGACAGATC ATGGCTCCTG CGTTCGCTCG 
TGTAATACTG ATACTTACGA AGTGGAAGAA AATGGTGTTC GGAAGTGTAA AAAATGTGAT 
GGGCTATGTA GCAAAGTGTG CAATGGCATT GGAATAGGTG AACTTAAAGG GATCCTATCC 
ATAAATGCCA CAAACATCGA CTCCTTCAAA AACTGTACGA AGATCAATGG GGATGTCAGC 
ATTCTTCCTG TTGCATTTCT AGGGGATGCC TTCACAAAGA CACTACCCCT TGACCCTAAG 
AAGCTGGATG TCTTTAGAAC AGTCAAAGAA ATATCAGGAT TTTTGTTGAT TCAGGCCTGG 
CCTGATAATG CTACTGATCT CTATGCTTTT GAAAATCTGG AGATTATCCG AGGCCGAACC 
AAGCAGCACG GCCAGTATTC CCTTGCTGTT GTTAACTTGA AAATACAGTC GTTGGGGCTG 
CGCTCCCTCA AGGAAATAAG TGATGGAGAC ATTGCCATTA TGAAGAACAA GAACCTCTGC 
TATGCTGACA CCATGAACTG GCGCAGCTTG TTTGCTACTC AGAGTCAGAA AACAAAAATT 
ATACAGAACA GAAATAAAAA TGATTGTACT GCTGACAGGC ATGTGTGTGA CCCGCTGTGC 
TCGGACGTGG GCTGCTGGGG CCCAGGGCCC TTCCACTGCT TTTCCTGCAG GTTTTTCAGT 
CGCCAGAAGG AGTGTGTAAA ACAGTGCAAC ATCCTGCAAG GGGAGCCACG TGAGTTTGAA 
AGAGACTCCA AATGCCTCCC CTGCCACTCA GAGTGTCTGG TACAGAACTC CACTGCATAC 
AACACAACCT GCTCTGGACC GGGCCCAGAC CACTGCATGA AGTGTGCCCA TTTTATAGAT 
GGTCCCCACT GTGTGAAGGC CTGCCCCGCT GGGGTCCTGG GTGAGAATGA TACCCTGGTC 
TGGAAGTATG CAGATGCCAA TGCTGTTTGC CAGCTCTGCC ATCCAAACTG TACACGAGGG 
TGCAAAGGAC CAGGTCTTGA AGGATGTCCA AATGGCTCCA AAACTCCATC TATCGCGGCT 
GGTGTTGTCG GAGGACTCCT GTGCCTGGTT GTGGTTGGTC TAGGCATCGG TCTTTACCTG 
CGGCGACGTC ATATCGTGCG GAAGCGCACC CTGCGCAGGC TGCTGCAAGA GAGGGAGCTT 
GTCGAACCAC TGACACCCAG TGGGGAGGCA CCAAACCAGG CCCACCTGAG AATTTTAAAG 
GAAACAGAAT TTAAAAAGGT CAAAGTTTTG GGCTCTGGAG CTTTTGGCAC TGTTTATAAG 
GGACTTTGGA TCCCAGAAGG GGAAAAGGTT AAAATTCCTG TTGCTATTAA AGAATTGAGA 
GAGGCTACAT CGCCAAAAGC CAACAAGGAA ATACTTGATG AAGCCTATGT GATGGCTAGT 
GTTGACAATC CTCATGTGTG CCGCTTGCTG GGAATCTGCC TCACTTCCAC TGTGCAGCTC 
ATCACCCAGC TTATGCCTTA TGGCTGCCTC CTTGACTACA TCCGAGAGCA CAAGGACAAC 
ATTGGCTCCC AGTACCTTCT CAACTGGTGT GTGCAGATTG CAAAGGGAAT GAACTACCTG 
GAGGAACGTC GCCTGGTGCA CCGTGACCTT GCTGCCAGGA ACGTCCTTGT TAAGACTCCA 
CAACATGTGA AAATCACAGA CTTTGGGCTG GCAAAGCTGC TTGGGGCAGA TGAGAAGGAG 
TATCACGCAG AGGGAGGCAA GGTTCCTATT AAATGGATGG CATTGGAGTC AATTTTACAC 
CGAATTTATA CTCATCAAAG TGATGTCTGG AGTTATGGTG TGACAGTTTG GGAGTTGATG 
ACATTTGGGT CCAAGCCTTA TGACGGGATC CCCGCAAGTG AAATCTCCTC CGTCTTGGAG 
AAGGGAGAGC GTTTGCCCCA GCCACCCATT TGTACCATTG ATGTGTACAT GATCATGGTC 
AAATGCTGGA TGATTGATGC AGACAGCCGT CCCAAGTTTC GTGAGCTGAT TGCAGAGTTC 
TCCAAAATGG CTCGTGACCC TCCCCGCTAT CTTGTTATAC AGGGAGATGA AAGGATGCAC 
TTGCCTAGCC CTACAGATTC CAAGTTTTAT CGCACCCTGA TGGAGGAGGA GGACATGGAA 
GACATTGTGG ATGCAGATGA GTATCTTGTC CCACACCAGG GCTTTTTCAA CAGCCCCTCT 
ACATCTCGGA CTCCTCTTCT GAGTTCATTG AGCGCTACTA GCAACAATTC TGCTACAAAC 
TGCATTGACA GAAATGGGGG GCACCCTGTG AGGGAAGACA GCTTTGTCCA GAGGTACAGC 
TCAGATCCAA CAGGAAACTT CTTGGAGGAG AGTATAGATG ATGGCTTCCT GCCTGCTCCA 
GAGTATGTAA ACCAGCTGAT GCCCAAGAAA CCATCTACTG CCATGGTCCA GAATCAAATC 
TACAACAACA TCTCTCTCAC AGCAATCTCA AAGCTCCCCA TGGACTCAAG ATACCAGAAT 
TCCCACAGCA CAGCTGTGGA CAACCCTGAG TATCTCAACA CTAACCAGTC CCCACTGGCC 
AAAACAGTCT TCGAGAGCTC TCCCTATTGG ATCCAATCAG GCAATCACCA AATAAATCTG 
GACAATCCTG ACTACCAGCA GGACTTTTTA CCAAATGAAA CAAAACCTAA TGGTCTCTTG 
AAAGTTCCTG CAGCAGAAAA CCCAGAGTAC TTGAGGGTAG CAGCACCAAA AAGTGAATAC 
ATTGAAGCTT CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015136814.1
CDS	78..3755
Translation	MGVRSPLSASGPRGAAVLVLLLLLLGRVALCSAVEEKKVCQGTNNKLTQLGHVEDHFTSLQRMYNNCEVVLSNLEITYVEHNRDLTFLKTIQEVAGYVLIALNMVDVIPLENLQIIRGNVLYDNSFALAVLSNYHMNKTQGLRELPMKRLSEILNGGVKISNNPKLCNMDTVLWNDIIDTSRKPLTVLDFASNLSSCPKCHPNCTEDHCWGAGEQNCQTLTKVICAQQCSGRCRGKVPSDCCHNQCAAGCTGPRESDCLACRKFRDDATCKDTCPPLVLYNPTTYQMDVNPEGKYSFGATCVRECPHNYVVTDHGSCVRSCNTDTYEVEENGVRKCKKCDGLCSKVCNGIGIGELKGILSINATNIDSFKNCTKINGDVSILPVAFLGDAFTKTLPLDPKKLDVFRTVKEISGFLLIQAWPDNATDLYAFENLEIIRGRTKQHGQYSLAVVNLKIQSLGLRSLKEISDGDIAIMKNKNLCYADTMNWRSLFATQSQKTKIIQNRNKNDCTADRHVCDPLCSDVGCWGPGPFHCFSCRFFSRQKECVKQCNILQGEPREFERDSKCLPCHSECLVQNSTAYNTTCSGPGPDHCMKCAHFIDGPHCVKACPAGVLGENDTLVWKYADANAVCQLCHPNCTRGCKGPGLEGCPNGSKTPSIAAGVVGGLLCLVVVGLGIGLYLRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQAHLRILKETEFKKVKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPYGCLLDYIREHKDNIGSQYLLNWCVQIAKGMNYLEERRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGADEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSVLEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIAEFSKMARDPPRYLVIQGDERMHLPSPTDSKFYRTLMEEEDMEDIVDADEYLVPHQGFFNSPSTSRTPLLSSLSATSNNSATNCIDRNGGHPVREDSFVQRYSSDPTGNFLEESIDDGFLPAPEYVNQLMPKKPSTAMVQNQIYNNISLTAISKLPMDSRYQNSHSTAVDNPEYLNTNQSPLAKTVFESSPYWIQSGNHQINLDNPDYQQDFLPNETKPNGLLKVPAAENPEYLRVAAPKSEYIEASA

Target ORF information:

RefSeq Version	XM_015281328.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus epidermal growth factor receptor (EGFR), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281328.2

ORF Insert Sequence:

ATGGGTGTAC GCAGCCCCCT GTCCGCCTCT GGGCCTCGCG GGGCCGCTGT CCTGGTGCTA 
CTGCTGCTGC TGCTGGGGCG CGTCGCGCTG TGTTCCGCCG TGGAGGAGAA GAAAGTTTGT 
CAAGGGACAA ATAACAAGTT GACCCAGCTG GGGCACGTGG AAGACCATTT CACCAGCCTG 
CAGAGAATGT ACAACAACTG CGAAGTGGTA CTGAGTAACC TGGAGATTAC CTACGTGGAG 
CATAATCGCG ATCTCACCTT CCTTAAGACC ATACAGGAGG TTGCAGGCTA TGTGCTCATT 
GCGCTTAACA TGGTGGACGT CATTCCCTTA GAAAACCTCC AGATTATCCG AGGGAATGTG 
CTTTATGACA ACTCTTTTGC CCTGGCAGTT TTATCCAATT ACCACATGAA TAAAACCCAG 
GGACTTCGAG AGCTGCCAAT GAAACGGCTA TCAGAAATTC TCAATGGAGG TGTTAAAATC 
AGCAACAACC CCAAACTGTG CAACATGGAC ACTGTTCTCT GGAATGACAT CATTGATACA 
AGCAGGAAGC CTCTCACAGT ACTTGACTTT GCAAGCAATC TTTCTTCTTG TCCAAAATGC 
CATCCGAACT GCACAGAAGA CCACTGCTGG GGTGCTGGTG AACAGAACTG CCAGACTTTA 
ACAAAAGTCA TCTGTGCCCA GCAATGCTCT GGCCGGTGCA GAGGAAAGGT GCCCAGTGAC 
TGCTGCCACA ATCAGTGTGC TGCAGGGTGC ACAGGACCTC GGGAGAGTGA CTGCCTGGCA 
TGCCGCAAGT TTCGGGATGA TGCTACCTGC AAGGACACAT GTCCCCCACT GGTCCTCTAT 
AACCCCACCA CCTATCAAAT GGATGTCAAC CCCGAGGGAA AATACAGCTT TGGAGCCACT 
TGTGTGAGGG AATGTCCACA CAACTATGTG GTGACAGATC ATGGCTCCTG CGTTCGCTCG 
TGTAATACTG ATACTTACGA AGTGGAAGAA AATGGTGTTC GGAAGTGTAA AAAATGTGAT 
GGGCTATGTA GCAAAGTGTG CAATGGCATT GGAATAGGTG AACTTAAAGG GATCCTATCC 
ATAAATGCCA CAAACATCGA CTCCTTCAAA AACTGTACGA AGATCAATGG GGATGTCAGC 
ATTCTTCCTG TTGCATTTCT AGGGGATGCC TTCACAAAGA CACTACCCCT TGACCCTAAG 
AAGCTGGATG TCTTTAGAAC AGTCAAAGAA ATATCAGGAT TTTTGTTGAT TCAGGCCTGG 
CCTGATAATG CTACTGATCT CTATGCTTTT GAAAATCTGG AGATTATCCG AGGCCGAACC 
AAGCAGCACG GCCAGTATTC CCTTGCTGTT GTTAACTTGA AAATACAGTC GTTGGGGCTG 
CGCTCCCTCA AGGAAATAAG TGATGGAGAC ATTGCCATTA TGAAGAACAA GAACCTCTGC 
TATGCTGACA CCATGAACTG GCGCAGCTTG TTTGCTACTC AGAGTCAGAA AACAAAAATT 
ATACAGAACA GAAATAAAAA TGATTGTACT GCTGACAGGC ATGTGTGTGA CCCGCTGTGC 
TCGGACGTGG GCTGCTGGGG CCCAGGGCCC TTCCACTGCT TTTCCTGCAG GTTTTTCAGT 
CGCCAGAAGG AGTGTGTAAA ACAGTGCAAC ATCCTGCAAG GGGAGCCACG TGAGTTTGAA 
AGAGACTCCA AATGCCTCCC CTGCCACTCA GAGTGTCTGG TACAGAACTC CACTGCATAC 
AACACAACCT GCTCTGGACC GGGCCCAGAC CACTGCATGA AGTGTGCCCA TTTTATAGAT 
GGTCCCCACT GTGTGAAGGC CTGCCCCGCT GGGGTCCTGG GTGAGAATGA TACCCTGGTC 
TGGAAGTATG CAGATGCCAA TGCTGTTTGC CAGCTCTGCC ATCCAAACTG TACACGAGGG 
TGCAAAGGAC CAGGTCTTGA AGGATGTCCA AATGGCTCCA AAACTCCATC TATCGCGGCT 
GGTGTTGTCG GAGGACTCCT GTGCCTGGTT GTGGTTGGTC TAGGCATCGG TCTTTACCTG 
CGGCGACGTC ATATCGTGCG GAAGCGCACC CTGCGCAGGC TGCTGCAAGA GAGGGAGCTT 
GTCGAACCAC TGACACCCAG TGGGGAGGCA CCAAACCAGG CCCACCTGAG AATTTTAAAG 
GAAACAGAAT TTAAAAAGGT CAAAGTTTTG GGCTCTGGAG CTTTTGGCAC TGTTTATAAG 
GGACTTTGGA TCCCAGAAGG GGAAAAGGTT AAAATTCCTG TTGCTATTAA AGAATTGAGA 
GAGGCTACAT CGCCAAAAGC CAACAAGGAA ATACTTGATG AAGCCTATGT GATGGCTAGT 
GTTGACAATC CTCATGTGTG CCGCTTGCTG GGAATCTGCC TCACTTCCAC TGTGCAGCTC 
ATCACCCAGC TTATGCCTTA TGGCTGCCTC CTTGACTACA TCCGAGAGCA CAAGGACAAC 
ATTGGCTCCC AGTACCTTCT CAACTGGTGT GTGCAGATTG CAAAGGGAAT GAACTACCTG 
GAGGAACGTC GCCTGGTGCA CCGTGACCTT GCTGCCAGGA ACGTCCTTGT TAAGACTCCA 
CAACATGTGA AAATCACAGA CTTTGGGCTG GCAAAGCTGC TTGGGGCAGA TGAGAAGGAG 
TATCACGCAG AGGGAGGCAA GGTTCCTATT AAATGGATGG CATTGGAGTC AATTTTACAC 
CGAATTTATA CTCATCAAAG TGATGTCTGG AGTTATGGTG TGACAGTTTG GGAGTTGATG 
ACATTTGGGT CCAAGCCTTA TGACGGGATC CCCGCAAGTG AAATCTCCTC CGTCTTGGAG 
AAGGGAGAGC GTTTGCCCCA GCCACCCATT TGTACCATTG ATGTGTACAT GATCATGGTC 
AAATGCTGGA TGATTGATGC AGACAGCCGT CCCAAGTTTC GTGAGCTGAT TGCAGAGTTC 
TCCAAAATGG CTCGTGACCC TCCCCGCTAT CTTGTTATAC AGGGAGATGA AAGGATGCAC 
TTGCCTAGCC CTACAGATTC CAAGTTTTAT CGCACCCTGA TGGAGGAGGA GGACATGGAA 
GACATTGTGG ATGCAGATGA GTATCTTGTC CCACACCAGG GCTTTTTCAA CAGCCCCTCT 
ACATCTCGGA CTCCTCTTCT GAGTTCATTG AGCGCTACTA GCAACAATTC TGCTACAAAC 
TGCATTGACA GAAATGGGGG GCACCCTGTG AGGGAAGACA GCTTTGTCCA GAGGTACAGC 
TCAGATCCAA CAGGAAACTT CTTGGAGGAG AGTATAGATG ATGGCTTCCT GCCTGCTCCA 
GAGTATGTAA ACCAGCTGAT GCCCAAGAAA CCATCTACTG CCATGGTCCA GAATCAAATC 
TACAACAACA TCTCTCTCAC AGCAATCTCA AAGCTCCCCA TGGACTCAAG ATACCAGAAT 
TCCCACAGCA CAGCTGTGGA CAACCCTGAG TATCTCAACA CTAACCAGTC CCCACTGGCC 
AAAACAGTCT TCGAGAGCTC TCCCTATTGG ATCCAATCAG GCAATCACCA AATAAATCTG 
GACAATCCTG ACTACCAGCA GGACTTTTTA CCAAATGAAA CAAAACCTAA TGGTCTCTTG 
AAAGTTCCTG CAGCAGAAAA CCCAGAGTAC TTGAGGGTAG CAGCACCAAA AAGTGAATAC 
ATTGAAGCTT CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGFR ( XM_015281328.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015136814.1 Gallus gallus epidermal growth factor receptor (EGFR), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa31512
Clone ID Related Accession (Same CDS sequence)	XM_015281328.2 , XM_015281328.1
Accession Version	XM_015281328.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	epidermal growth factor receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGTAC GCAGCCCCCT GTCCGCCTCT GGGCCTCGCG GGGCCGCTGT CCTGGTGCTA 
CTGCTGCTGC TGCTGGGGCG CGTCGCGCTG TGTTCCGCCG TGGAGGAGAA GAAAGTTTGT 
CAAGGGACAA ATAACAAGTT GACCCAGCTG GGGCACGTGG AAGACCATTT CACCAGCCTG 
CAGAGAATGT ACAACAACTG CGAAGTGGTA CTGAGTAACC TGGAGATTAC CTACGTGGAG 
CATAATCGCG ATCTCACCTT CCTTAAGACC ATACAGGAGG TTGCAGGCTA TGTGCTCATT 
GCGCTTAACA TGGTGGACGT CATTCCCTTA GAAAACCTCC AGATTATCCG AGGGAATGTG 
CTTTATGACA ACTCTTTTGC CCTGGCAGTT TTATCCAATT ACCACATGAA TAAAACCCAG 
GGACTTCGAG AGCTGCCAAT GAAACGGCTA TCAGAAATTC TCAATGGAGG TGTTAAAATC 
AGCAACAACC CCAAACTGTG CAACATGGAC ACTGTTCTCT GGAATGACAT CATTGATACA 
AGCAGGAAGC CTCTCACAGT ACTTGACTTT GCAAGCAATC TTTCTTCTTG TCCAAAATGC 
CATCCGAACT GCACAGAAGA CCACTGCTGG GGTGCTGGTG AACAGAACTG CCAGACTTTA 
ACAAAAGTCA TCTGTGCCCA GCAATGCTCT GGCCGGTGCA GAGGAAAGGT GCCCAGTGAC 
TGCTGCCACA ATCAGTGTGC TGCAGGGTGC ACAGGACCTC GGGAGAGTGA CTGCCTGGCA 
TGCCGCAAGT TTCGGGATGA TGCTACCTGC AAGGACACAT GTCCCCCACT GGTCCTCTAT 
AACCCCACCA CCTATCAAAT GGATGTCAAC CCCGAGGGAA AATACAGCTT TGGAGCCACT 
TGTGTGAGGG AATGTCCACA CAACTATGTG GTGACAGATC ATGGCTCCTG CGTTCGCTCG 
TGTAATACTG ATACTTACGA AGTGGAAGAA AATGGTGTTC GGAAGTGTAA AAAATGTGAT 
GGGCTATGTA GCAAAGTGTG CAATGGCATT GGAATAGGTG AACTTAAAGG GATCCTATCC 
ATAAATGCCA CAAACATCGA CTCCTTCAAA AACTGTACGA AGATCAATGG GGATGTCAGC 
ATTCTTCCTG TTGCATTTCT AGGGGATGCC TTCACAAAGA CACTACCCCT TGACCCTAAG 
AAGCTGGATG TCTTTAGAAC AGTCAAAGAA ATATCAGGAT TTTTGTTGAT TCAGGCCTGG 
CCTGATAATG CTACTGATCT CTATGCTTTT GAAAATCTGG AGATTATCCG AGGCCGAACC 
AAGCAGCACG GCCAGTATTC CCTTGCTGTT GTTAACTTGA AAATACAGTC GTTGGGGCTG 
CGCTCCCTCA AGGAAATAAG TGATGGAGAC ATTGCCATTA TGAAGAACAA GAACCTCTGC 
TATGCTGACA CCATGAACTG GCGCAGCTTG TTTGCTACTC AGAGTCAGAA AACAAAAATT 
ATACAGAACA GAAATAAAAA TGATTGTACT GCTGACAGGC ATGTGTGTGA CCCGCTGTGC 
TCGGACGTGG GCTGCTGGGG CCCAGGGCCC TTCCACTGCT TTTCCTGCAG GTTTTTCAGT 
CGCCAGAAGG AGTGTGTAAA ACAGTGCAAC ATCCTGCAAG GGGAGCCACG TGAGTTTGAA 
AGAGACTCCA AATGCCTCCC CTGCCACTCA GAGTGTCTGG TACAGAACTC CACTGCATAC 
AACACAACCT GCTCTGGACC GGGCCCAGAC CACTGCATGA AGTGTGCCCA TTTTATAGAT 
GGTCCCCACT GTGTGAAGGC CTGCCCCGCT GGGGTCCTGG GTGAGAATGA TACCCTGGTC 
TGGAAGTATG CAGATGCCAA TGCTGTTTGC CAGCTCTGCC ATCCAAACTG TACACGAGGG 
TGCAAAGGAC CAGGTCTTGA AGGATGTCCA AATGGCTCCA AAACTCCATC TATCGCGGCT 
GGTGTTGTCG GAGGACTCCT GTGCCTGGTT GTGGTTGGTC TAGGCATCGG TCTTTACCTG 
CGGCGACGTC ATATCGTGCG GAAGCGCACC CTGCGCAGGC TGCTGCAAGA GAGGGAGCTT 
GTCGAACCAC TGACACCCAG TGGGGAGGCA CCAAACCAGG CCCACCTGAG AATTTTAAAG 
GAAACAGAAT TTAAAAAGGT CAAAGTTTTG GGCTCTGGAG CTTTTGGCAC TGTTTATAAG 
GGACTTTGGA TCCCAGAAGG GGAAAAGGTT AAAATTCCTG TTGCTATTAA AGAATTGAGA 
GAGGCTACAT CGCCAAAAGC CAACAAGGAA ATACTTGATG AAGCCTATGT GATGGCTAGT 
GTTGACAATC CTCATGTGTG CCGCTTGCTG GGAATCTGCC TCACTTCCAC TGTGCAGCTC 
ATCACCCAGC TTATGCCTTA TGGCTGCCTC CTTGACTACA TCCGAGAGCA CAAGGACAAC 
ATTGGCTCCC AGTACCTTCT CAACTGGTGT GTGCAGATTG CAAAGGGAAT GAACTACCTG 
GAGGAACGTC GCCTGGTGCA CCGTGACCTT GCTGCCAGGA ACGTCCTTGT TAAGACTCCA 
CAACATGTGA AAATCACAGA CTTTGGGCTG GCAAAGCTGC TTGGGGCAGA TGAGAAGGAG 
TATCACGCAG AGGGAGGCAA GGTTCCTATT AAATGGATGG CATTGGAGTC AATTTTACAC 
CGAATTTATA CTCATCAAAG TGATGTCTGG AGTTATGGTG TGACAGTTTG GGAGTTGATG 
ACATTTGGGT CCAAGCCTTA TGACGGGATC CCCGCAAGTG AAATCTCCTC CGTCTTGGAG 
AAGGGAGAGC GTTTGCCCCA GCCACCCATT TGTACCATTG ATGTGTACAT GATCATGGTC 
AAATGCTGGA TGATTGATGC AGACAGCCGT CCCAAGTTTC GTGAGCTGAT TGCAGAGTTC 
TCCAAAATGG CTCGTGACCC TCCCCGCTAT CTTGTTATAC AGGGAGATGA AAGGATGCAC 
TTGCCTAGCC CTACAGATTC CAAGTTTTAT CGCACCCTGA TGGAGGAGGA GGACATGGAA 
GACATTGTGG ATGCAGATGA GTATCTTGTC CCACACCAGG GCTTTTTCAA CAGCCCCTCT 
ACATCTCGGA CTCCTCTTCT GAGTTCATTG AGCGCTACTA GCAACAATTC TGCTACAAAC 
TGCATTGACA GAAATGGGGG GCACCCTGTG AGGGAAGACA GCTTTGTCCA GAGGTACAGC 
TCAGATCCAA CAGGAAACTT CTTGGAGGAG AGTATAGATG ATGGCTTCCT GCCTGCTCCA 
GAGTATGTAA ACCAGCTGAT GCCCAAGAAA CCATCTACTG CCATGGTCCA GAATCAAATC 
TACAACAACA TCTCTCTCAC AGCAATCTCA AAGCTCCCCA TGGACTCAAG ATACCAGAAT 
TCCCACAGCA CAGCTGTGGA CAACCCTGAG TATCTCAACA CTAACCAGTC CCCACTGGCC 
AAAACAGTCT TCGAGAGCTC TCCCTATTGG ATCCAATCAG GCAATCACCA AATAAATCTG 
GACAATCCTG ACTACCAGCA GGACTTTTTA CCAAATGAAA CAAAACCTAA TGGTCTCTTG 
AAAGTTCCTG CAGCAGAAAA CCCAGAGTAC TTGAGGGTAG CAGCACCAAA AAGTGAATAC 
ATTGAAGCTT CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015136814.1
CDS	379..4056
Translation	MGVRSPLSASGPRGAAVLVLLLLLLGRVALCSAVEEKKVCQGTNNKLTQLGHVEDHFTSLQRMYNNCEVVLSNLEITYVEHNRDLTFLKTIQEVAGYVLIALNMVDVIPLENLQIIRGNVLYDNSFALAVLSNYHMNKTQGLRELPMKRLSEILNGGVKISNNPKLCNMDTVLWNDIIDTSRKPLTVLDFASNLSSCPKCHPNCTEDHCWGAGEQNCQTLTKVICAQQCSGRCRGKVPSDCCHNQCAAGCTGPRESDCLACRKFRDDATCKDTCPPLVLYNPTTYQMDVNPEGKYSFGATCVRECPHNYVVTDHGSCVRSCNTDTYEVEENGVRKCKKCDGLCSKVCNGIGIGELKGILSINATNIDSFKNCTKINGDVSILPVAFLGDAFTKTLPLDPKKLDVFRTVKEISGFLLIQAWPDNATDLYAFENLEIIRGRTKQHGQYSLAVVNLKIQSLGLRSLKEISDGDIAIMKNKNLCYADTMNWRSLFATQSQKTKIIQNRNKNDCTADRHVCDPLCSDVGCWGPGPFHCFSCRFFSRQKECVKQCNILQGEPREFERDSKCLPCHSECLVQNSTAYNTTCSGPGPDHCMKCAHFIDGPHCVKACPAGVLGENDTLVWKYADANAVCQLCHPNCTRGCKGPGLEGCPNGSKTPSIAAGVVGGLLCLVVVGLGIGLYLRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQAHLRILKETEFKKVKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPYGCLLDYIREHKDNIGSQYLLNWCVQIAKGMNYLEERRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGADEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSVLEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIAEFSKMARDPPRYLVIQGDERMHLPSPTDSKFYRTLMEEEDMEDIVDADEYLVPHQGFFNSPSTSRTPLLSSLSATSNNSATNCIDRNGGHPVREDSFVQRYSSDPTGNFLEESIDDGFLPAPEYVNQLMPKKPSTAMVQNQIYNNISLTAISKLPMDSRYQNSHSTAVDNPEYLNTNQSPLAKTVFESSPYWIQSGNHQINLDNPDYQQDFLPNETKPNGLLKVPAAENPEYLRVAAPKSEYIEASA

Target ORF information:

RefSeq Version	XM_015281328.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus epidermal growth factor receptor (EGFR), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281328.1

ORF Insert Sequence:

ATGGGTGTAC GCAGCCCCCT GTCCGCCTCT GGGCCTCGCG GGGCCGCTGT CCTGGTGCTA 
CTGCTGCTGC TGCTGGGGCG CGTCGCGCTG TGTTCCGCCG TGGAGGAGAA GAAAGTTTGT 
CAAGGGACAA ATAACAAGTT GACCCAGCTG GGGCACGTGG AAGACCATTT CACCAGCCTG 
CAGAGAATGT ACAACAACTG CGAAGTGGTA CTGAGTAACC TGGAGATTAC CTACGTGGAG 
CATAATCGCG ATCTCACCTT CCTTAAGACC ATACAGGAGG TTGCAGGCTA TGTGCTCATT 
GCGCTTAACA TGGTGGACGT CATTCCCTTA GAAAACCTCC AGATTATCCG AGGGAATGTG 
CTTTATGACA ACTCTTTTGC CCTGGCAGTT TTATCCAATT ACCACATGAA TAAAACCCAG 
GGACTTCGAG AGCTGCCAAT GAAACGGCTA TCAGAAATTC TCAATGGAGG TGTTAAAATC 
AGCAACAACC CCAAACTGTG CAACATGGAC ACTGTTCTCT GGAATGACAT CATTGATACA 
AGCAGGAAGC CTCTCACAGT ACTTGACTTT GCAAGCAATC TTTCTTCTTG TCCAAAATGC 
CATCCGAACT GCACAGAAGA CCACTGCTGG GGTGCTGGTG AACAGAACTG CCAGACTTTA 
ACAAAAGTCA TCTGTGCCCA GCAATGCTCT GGCCGGTGCA GAGGAAAGGT GCCCAGTGAC 
TGCTGCCACA ATCAGTGTGC TGCAGGGTGC ACAGGACCTC GGGAGAGTGA CTGCCTGGCA 
TGCCGCAAGT TTCGGGATGA TGCTACCTGC AAGGACACAT GTCCCCCACT GGTCCTCTAT 
AACCCCACCA CCTATCAAAT GGATGTCAAC CCCGAGGGAA AATACAGCTT TGGAGCCACT 
TGTGTGAGGG AATGTCCACA CAACTATGTG GTGACAGATC ATGGCTCCTG CGTTCGCTCG 
TGTAATACTG ATACTTACGA AGTGGAAGAA AATGGTGTTC GGAAGTGTAA AAAATGTGAT 
GGGCTATGTA GCAAAGTGTG CAATGGCATT GGAATAGGTG AACTTAAAGG GATCCTATCC 
ATAAATGCCA CAAACATCGA CTCCTTCAAA AACTGTACGA AGATCAATGG GGATGTCAGC 
ATTCTTCCTG TTGCATTTCT AGGGGATGCC TTCACAAAGA CACTACCCCT TGACCCTAAG 
AAGCTGGATG TCTTTAGAAC AGTCAAAGAA ATATCAGGAT TTTTGTTGAT TCAGGCCTGG 
CCTGATAATG CTACTGATCT CTATGCTTTT GAAAATCTGG AGATTATCCG AGGCCGAACC 
AAGCAGCACG GCCAGTATTC CCTTGCTGTT GTTAACTTGA AAATACAGTC GTTGGGGCTG 
CGCTCCCTCA AGGAAATAAG TGATGGAGAC ATTGCCATTA TGAAGAACAA GAACCTCTGC 
TATGCTGACA CCATGAACTG GCGCAGCTTG TTTGCTACTC AGAGTCAGAA AACAAAAATT 
ATACAGAACA GAAATAAAAA TGATTGTACT GCTGACAGGC ATGTGTGTGA CCCGCTGTGC 
TCGGACGTGG GCTGCTGGGG CCCAGGGCCC TTCCACTGCT TTTCCTGCAG GTTTTTCAGT 
CGCCAGAAGG AGTGTGTAAA ACAGTGCAAC ATCCTGCAAG GGGAGCCACG TGAGTTTGAA 
AGAGACTCCA AATGCCTCCC CTGCCACTCA GAGTGTCTGG TACAGAACTC CACTGCATAC 
AACACAACCT GCTCTGGACC GGGCCCAGAC CACTGCATGA AGTGTGCCCA TTTTATAGAT 
GGTCCCCACT GTGTGAAGGC CTGCCCCGCT GGGGTCCTGG GTGAGAATGA TACCCTGGTC 
TGGAAGTATG CAGATGCCAA TGCTGTTTGC CAGCTCTGCC ATCCAAACTG TACACGAGGG 
TGCAAAGGAC CAGGTCTTGA AGGATGTCCA AATGGCTCCA AAACTCCATC TATCGCGGCT 
GGTGTTGTCG GAGGACTCCT GTGCCTGGTT GTGGTTGGTC TAGGCATCGG TCTTTACCTG 
CGGCGACGTC ATATCGTGCG GAAGCGCACC CTGCGCAGGC TGCTGCAAGA GAGGGAGCTT 
GTCGAACCAC TGACACCCAG TGGGGAGGCA CCAAACCAGG CCCACCTGAG AATTTTAAAG 
GAAACAGAAT TTAAAAAGGT CAAAGTTTTG GGCTCTGGAG CTTTTGGCAC TGTTTATAAG 
GGACTTTGGA TCCCAGAAGG GGAAAAGGTT AAAATTCCTG TTGCTATTAA AGAATTGAGA 
GAGGCTACAT CGCCAAAAGC CAACAAGGAA ATACTTGATG AAGCCTATGT GATGGCTAGT 
GTTGACAATC CTCATGTGTG CCGCTTGCTG GGAATCTGCC TCACTTCCAC TGTGCAGCTC 
ATCACCCAGC TTATGCCTTA TGGCTGCCTC CTTGACTACA TCCGAGAGCA CAAGGACAAC 
ATTGGCTCCC AGTACCTTCT CAACTGGTGT GTGCAGATTG CAAAGGGAAT GAACTACCTG 
GAGGAACGTC GCCTGGTGCA CCGTGACCTT GCTGCCAGGA ACGTCCTTGT TAAGACTCCA 
CAACATGTGA AAATCACAGA CTTTGGGCTG GCAAAGCTGC TTGGGGCAGA TGAGAAGGAG 
TATCACGCAG AGGGAGGCAA GGTTCCTATT AAATGGATGG CATTGGAGTC AATTTTACAC 
CGAATTTATA CTCATCAAAG TGATGTCTGG AGTTATGGTG TGACAGTTTG GGAGTTGATG 
ACATTTGGGT CCAAGCCTTA TGACGGGATC CCCGCAAGTG AAATCTCCTC CGTCTTGGAG 
AAGGGAGAGC GTTTGCCCCA GCCACCCATT TGTACCATTG ATGTGTACAT GATCATGGTC 
AAATGCTGGA TGATTGATGC AGACAGCCGT CCCAAGTTTC GTGAGCTGAT TGCAGAGTTC 
TCCAAAATGG CTCGTGACCC TCCCCGCTAT CTTGTTATAC AGGGAGATGA AAGGATGCAC 
TTGCCTAGCC CTACAGATTC CAAGTTTTAT CGCACCCTGA TGGAGGAGGA GGACATGGAA 
GACATTGTGG ATGCAGATGA GTATCTTGTC CCACACCAGG GCTTTTTCAA CAGCCCCTCT 
ACATCTCGGA CTCCTCTTCT GAGTTCATTG AGCGCTACTA GCAACAATTC TGCTACAAAC 
TGCATTGACA GAAATGGGGG GCACCCTGTG AGGGAAGACA GCTTTGTCCA GAGGTACAGC 
TCAGATCCAA CAGGAAACTT CTTGGAGGAG AGTATAGATG ATGGCTTCCT GCCTGCTCCA 
GAGTATGTAA ACCAGCTGAT GCCCAAGAAA CCATCTACTG CCATGGTCCA GAATCAAATC 
TACAACAACA TCTCTCTCAC AGCAATCTCA AAGCTCCCCA TGGACTCAAG ATACCAGAAT 
TCCCACAGCA CAGCTGTGGA CAACCCTGAG TATCTCAACA CTAACCAGTC CCCACTGGCC 
AAAACAGTCT TCGAGAGCTC TCCCTATTGG ATCCAATCAG GCAATCACCA AATAAATCTG 
GACAATCCTG ACTACCAGCA GGACTTTTTA CCAAATGAAA CAAAACCTAA TGGTCTCTTG 
AAAGTTCCTG CAGCAGAAAA CCCAGAGTAC TTGAGGGTAG CAGCACCAAA AAGTGAATAC 
ATTGAAGCTT CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGFR ( XM_015281329.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015136815.1 Gallus gallus epidermal growth factor receptor (EGFR), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa31513
Clone ID Related Accession (Same CDS sequence)	XM_015281329.1
Accession Version	XM_015281329.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	epidermal growth factor receptor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCAAC TGCTGGCATC CAGCAGTCTC TGTGCTCAGT ACAGCCTCCT TTTTTATATT 
CTTTGTCAAG GGACAAATAA CAAGTTGACC CAGCTGGGGC ACGTGGAAGA CCATTTCACC 
AGCCTGCAGA GAATGTACAA CAACTGCGAA GTGGTACTGA GTAACCTGGA GATTACCTAC 
GTGGAGCATA ATCGCGATCT CACCTTCCTT AAGACCATAC AGGAGGTTGC AGGCTATGTG 
CTCATTGCGC TTAACATGGT GGACGTCATT CCCTTAGAAA ACCTCCAGAT TATCCGAGGG 
AATGTGCTTT ATGACAACTC TTTTGCCCTG GCAGTTTTAT CCAATTACCA CATGAATAAA 
ACCCAGGGAC TTCGAGAGCT GCCAATGAAA CGGCTATCAG AAATTCTCAA TGGAGGTGTT 
AAAATCAGCA ACAACCCCAA ACTGTGCAAC ATGGACACTG TTCTCTGGAA TGACATCATT 
GATACAAGCA GGAAGCCTCT CACAGTACTT GACTTTGCAA GCAATCTTTC TTCTTGTCCA 
AAATGCCATC CGAACTGCAC AGAAGACCAC TGCTGGGGTG CTGGTGAACA GAACTGCCAG 
ACTTTAACAA AAGTCATCTG TGCCCAGCAA TGCTCTGGCC GGTGCAGAGG AAAGGTGCCC 
AGTGACTGCT GCCACAATCA GTGTGCTGCA GGGTGCACAG GACCTCGGGA GAGTGACTGC 
CTGGCATGCC GCAAGTTTCG GGATGATGCT ACCTGCAAGG ACACATGTCC CCCACTGGTC 
CTCTATAACC CCACCACCTA TCAAATGGAT GTCAACCCCG AGGGAAAATA CAGCTTTGGA 
GCCACTTGTG TGAGGGAATG TCCACACAAC TATGTGGTGA CAGATCATGG CTCCTGCGTT 
CGCTCGTGTA ATACTGATAC TTACGAAGTG GAAGAAAATG GTGTTCGGAA GTGTAAAAAA 
TGTGATGGGC TATGTAGCAA AGTGTGCAAT GGCATTGGAA TAGGTGAACT TAAAGGGATC 
CTATCCATAA ATGCCACAAA CATCGACTCC TTCAAAAACT GTACGAAGAT CAATGGGGAT 
GTCAGCATTC TTCCTGTTGC ATTTCTAGGG GATGCCTTCA CAAAGACACT ACCCCTTGAC 
CCTAAGAAGC TGGATGTCTT TAGAACAGTC AAAGAAATAT CAGGATTTTT GTTGATTCAG 
GCCTGGCCTG ATAATGCTAC TGATCTCTAT GCTTTTGAAA ATCTGGAGAT TATCCGAGGC 
CGAACCAAGC AGCACGGCCA GTATTCCCTT GCTGTTGTTA ACTTGAAAAT ACAGTCGTTG 
GGGCTGCGCT CCCTCAAGGA AATAAGTGAT GGAGACATTG CCATTATGAA GAACAAGAAC 
CTCTGCTATG CTGACACCAT GAACTGGCGC AGCTTGTTTG CTACTCAGAG TCAGAAAACA 
AAAATTATAC AGAACAGAAA TAAAAATGAT TGTACTGCTG ACAGGCATGT GTGTGACCCG 
CTGTGCTCGG ACGTGGGCTG CTGGGGCCCA GGGCCCTTCC ACTGCTTTTC CTGCAGGTTT 
TTCAGTCGCC AGAAGGAGTG TGTAAAACAG TGCAACATCC TGCAAGGGGA GCCACGTGAG 
TTTGAAAGAG ACTCCAAATG CCTCCCCTGC CACTCAGAGT GTCTGGTACA GAACTCCACT 
GCATACAACA CAACCTGCTC TGGACCGGGC CCAGACCACT GCATGAAGTG TGCCCATTTT 
ATAGATGGTC CCCACTGTGT GAAGGCCTGC CCCGCTGGGG TCCTGGGTGA GAATGATACC 
CTGGTCTGGA AGTATGCAGA TGCCAATGCT GTTTGCCAGC TCTGCCATCC AAACTGTACA 
CGAGGGTGCA AAGGACCAGG TCTTGAAGGA TGTCCAAATG GCTCCAAAAC TCCATCTATC 
GCGGCTGGTG TTGTCGGAGG ACTCCTGTGC CTGGTTGTGG TTGGTCTAGG CATCGGTCTT 
TACCTGCGGC GACGTCATAT CGTGCGGAAG CGCACCCTGC GCAGGCTGCT GCAAGAGAGG 
GAGCTTGTCG AACCACTGAC ACCCAGTGGG GAGGCACCAA ACCAGGCCCA CCTGAGAATT 
TTAAAGGAAA CAGAATTTAA AAAGGTCAAA GTTTTGGGCT CTGGAGCTTT TGGCACTGTT 
TATAAGGGAC TTTGGATCCC AGAAGGGGAA AAGGTTAAAA TTCCTGTTGC TATTAAAGAA 
TTGAGAGAGG CTACATCGCC AAAAGCCAAC AAGGAAATAC TTGATGAAGC CTATGTGATG 
GCTAGTGTTG ACAATCCTCA TGTGTGCCGC TTGCTGGGAA TCTGCCTCAC TTCCACTGTG 
CAGCTCATCA CCCAGCTTAT GCCTTATGGC TGCCTCCTTG ACTACATCCG AGAGCACAAG 
GACAACATTG GCTCCCAGTA CCTTCTCAAC TGGTGTGTGC AGATTGCAAA GGGAATGAAC 
TACCTGGAGG AACGTCGCCT GGTGCACCGT GACCTTGCTG CCAGGAACGT CCTTGTTAAG 
ACTCCACAAC ATGTGAAAAT CACAGACTTT GGGCTGGCAA AGCTGCTTGG GGCAGATGAG 
AAGGAGTATC ACGCAGAGGG AGGCAAGGTT CCTATTAAAT GGATGGCATT GGAGTCAATT 
TTACACCGAA TTTATACTCA TCAAAGTGAT GTCTGGAGTT ATGGTGTGAC AGTTTGGGAG 
TTGATGACAT TTGGGTCCAA GCCTTATGAC GGGATCCCCG CAAGTGAAAT CTCCTCCGTC 
TTGGAGAAGG GAGAGCGTTT GCCCCAGCCA CCCATTTGTA CCATTGATGT GTACATGATC 
ATGGTCAAAT GCTGGATGAT TGATGCAGAC AGCCGTCCCA AGTTTCGTGA GCTGATTGCA 
GAGTTCTCCA AAATGGCTCG TGACCCTCCC CGCTATCTTG TTATACAGGG AGATGAAAGG 
ATGCACTTGC CTAGCCCTAC AGATTCCAAG TTTTATCGCA CCCTGATGGA GGAGGAGGAC 
ATGGAAGACA TTGTGGATGC AGATGAGTAT CTTGTCCCAC ACCAGGGCTT TTTCAACAGC 
CCCTCTACAT CTCGGACTCC TCTTCTGAGT TCATTGAGCG CTACTAGCAA CAATTCTGCT 
ACAAACTGCA TTGACAGAAA TGGGCAGGGG CACCCTGTGA GGGAAGACAG CTTTGTCCAG 
AGGTACAGCT CAGATCCAAC AGGAAACTTC TTGGAGGAGA GTATAGATGA TGGCTTCCTG 
CCTGCTCCAG AGTATGTAAA CCAGCTGATG CCCAAGAAAC CATCTACTGC CATGGTCCAG 
AATCAAATCT ACAACAACAT CTCTCTCACA GCAATCTCAA AGCTCCCCAT GGACTCAAGA 
TACCAGAATT CCCACAGCAC AGCTGTGGAC AACCCTGAGT ATCTCAACAC TAACCAGTCC 
CCACTGGCCA AAACAGTCTT CGAGAGCTCT CCCTATTGGA TCCAATCAGG CAATCACCAA 
ATAAATCTGG ACAATCCTGA CTACCAGCAG GACTTTTTAC CAAATGAAAC AAAACCTAAT 
GGTCTCTTGA AAGTTCCTGC AGCAGAAAAC CCAGAGTACT TGAGGGTAGC AGCACCAAAA 
AGTGAATACA TTGAAGCTTC AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015136815.1
CDS	21..3647
Translation	MQQLLASSSLCAQYSLLFYILCQGTNNKLTQLGHVEDHFTSLQRMYNNCEVVLSNLEITYVEHNRDLTFLKTIQEVAGYVLIALNMVDVIPLENLQIIRGNVLYDNSFALAVLSNYHMNKTQGLRELPMKRLSEILNGGVKISNNPKLCNMDTVLWNDIIDTSRKPLTVLDFASNLSSCPKCHPNCTEDHCWGAGEQNCQTLTKVICAQQCSGRCRGKVPSDCCHNQCAAGCTGPRESDCLACRKFRDDATCKDTCPPLVLYNPTTYQMDVNPEGKYSFGATCVRECPHNYVVTDHGSCVRSCNTDTYEVEENGVRKCKKCDGLCSKVCNGIGIGELKGILSINATNIDSFKNCTKINGDVSILPVAFLGDAFTKTLPLDPKKLDVFRTVKEISGFLLIQAWPDNATDLYAFENLEIIRGRTKQHGQYSLAVVNLKIQSLGLRSLKEISDGDIAIMKNKNLCYADTMNWRSLFATQSQKTKIIQNRNKNDCTADRHVCDPLCSDVGCWGPGPFHCFSCRFFSRQKECVKQCNILQGEPREFERDSKCLPCHSECLVQNSTAYNTTCSGPGPDHCMKCAHFIDGPHCVKACPAGVLGENDTLVWKYADANAVCQLCHPNCTRGCKGPGLEGCPNGSKTPSIAAGVVGGLLCLVVVGLGIGLYLRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQAHLRILKETEFKKVKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPYGCLLDYIREHKDNIGSQYLLNWCVQIAKGMNYLEERRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGADEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSVLEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIAEFSKMARDPPRYLVIQGDERMHLPSPTDSKFYRTLMEEEDMEDIVDADEYLVPHQGFFNSPSTSRTPLLSSLSATSNNSATNCIDRNGQGHPVREDSFVQRYSSDPTGNFLEESIDDGFLPAPEYVNQLMPKKPSTAMVQNQIYNNISLTAISKLPMDSRYQNSHSTAVDNPEYLNTNQSPLAKTVFESSPYWIQSGNHQINLDNPDYQQDFLPNETKPNGLLKVPAAENPEYLRVAAPKSEYIEASA

Target ORF information:

RefSeq Version	XM_015281329.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus epidermal growth factor receptor (EGFR), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281329.1

ORF Insert Sequence:

ATGCAGCAAC TGCTGGCATC CAGCAGTCTC TGTGCTCAGT ACAGCCTCCT TTTTTATATT 
CTTTGTCAAG GGACAAATAA CAAGTTGACC CAGCTGGGGC ACGTGGAAGA CCATTTCACC 
AGCCTGCAGA GAATGTACAA CAACTGCGAA GTGGTACTGA GTAACCTGGA GATTACCTAC 
GTGGAGCATA ATCGCGATCT CACCTTCCTT AAGACCATAC AGGAGGTTGC AGGCTATGTG 
CTCATTGCGC TTAACATGGT GGACGTCATT CCCTTAGAAA ACCTCCAGAT TATCCGAGGG 
AATGTGCTTT ATGACAACTC TTTTGCCCTG GCAGTTTTAT CCAATTACCA CATGAATAAA 
ACCCAGGGAC TTCGAGAGCT GCCAATGAAA CGGCTATCAG AAATTCTCAA TGGAGGTGTT 
AAAATCAGCA ACAACCCCAA ACTGTGCAAC ATGGACACTG TTCTCTGGAA TGACATCATT 
GATACAAGCA GGAAGCCTCT CACAGTACTT GACTTTGCAA GCAATCTTTC TTCTTGTCCA 
AAATGCCATC CGAACTGCAC AGAAGACCAC TGCTGGGGTG CTGGTGAACA GAACTGCCAG 
ACTTTAACAA AAGTCATCTG TGCCCAGCAA TGCTCTGGCC GGTGCAGAGG AAAGGTGCCC 
AGTGACTGCT GCCACAATCA GTGTGCTGCA GGGTGCACAG GACCTCGGGA GAGTGACTGC 
CTGGCATGCC GCAAGTTTCG GGATGATGCT ACCTGCAAGG ACACATGTCC CCCACTGGTC 
CTCTATAACC CCACCACCTA TCAAATGGAT GTCAACCCCG AGGGAAAATA CAGCTTTGGA 
GCCACTTGTG TGAGGGAATG TCCACACAAC TATGTGGTGA CAGATCATGG CTCCTGCGTT 
CGCTCGTGTA ATACTGATAC TTACGAAGTG GAAGAAAATG GTGTTCGGAA GTGTAAAAAA 
TGTGATGGGC TATGTAGCAA AGTGTGCAAT GGCATTGGAA TAGGTGAACT TAAAGGGATC 
CTATCCATAA ATGCCACAAA CATCGACTCC TTCAAAAACT GTACGAAGAT CAATGGGGAT 
GTCAGCATTC TTCCTGTTGC ATTTCTAGGG GATGCCTTCA CAAAGACACT ACCCCTTGAC 
CCTAAGAAGC TGGATGTCTT TAGAACAGTC AAAGAAATAT CAGGATTTTT GTTGATTCAG 
GCCTGGCCTG ATAATGCTAC TGATCTCTAT GCTTTTGAAA ATCTGGAGAT TATCCGAGGC 
CGAACCAAGC AGCACGGCCA GTATTCCCTT GCTGTTGTTA ACTTGAAAAT ACAGTCGTTG 
GGGCTGCGCT CCCTCAAGGA AATAAGTGAT GGAGACATTG CCATTATGAA GAACAAGAAC 
CTCTGCTATG CTGACACCAT GAACTGGCGC AGCTTGTTTG CTACTCAGAG TCAGAAAACA 
AAAATTATAC AGAACAGAAA TAAAAATGAT TGTACTGCTG ACAGGCATGT GTGTGACCCG 
CTGTGCTCGG ACGTGGGCTG CTGGGGCCCA GGGCCCTTCC ACTGCTTTTC CTGCAGGTTT 
TTCAGTCGCC AGAAGGAGTG TGTAAAACAG TGCAACATCC TGCAAGGGGA GCCACGTGAG 
TTTGAAAGAG ACTCCAAATG CCTCCCCTGC CACTCAGAGT GTCTGGTACA GAACTCCACT 
GCATACAACA CAACCTGCTC TGGACCGGGC CCAGACCACT GCATGAAGTG TGCCCATTTT 
ATAGATGGTC CCCACTGTGT GAAGGCCTGC CCCGCTGGGG TCCTGGGTGA GAATGATACC 
CTGGTCTGGA AGTATGCAGA TGCCAATGCT GTTTGCCAGC TCTGCCATCC AAACTGTACA 
CGAGGGTGCA AAGGACCAGG TCTTGAAGGA TGTCCAAATG GCTCCAAAAC TCCATCTATC 
GCGGCTGGTG TTGTCGGAGG ACTCCTGTGC CTGGTTGTGG TTGGTCTAGG CATCGGTCTT 
TACCTGCGGC GACGTCATAT CGTGCGGAAG CGCACCCTGC GCAGGCTGCT GCAAGAGAGG 
GAGCTTGTCG AACCACTGAC ACCCAGTGGG GAGGCACCAA ACCAGGCCCA CCTGAGAATT 
TTAAAGGAAA CAGAATTTAA AAAGGTCAAA GTTTTGGGCT CTGGAGCTTT TGGCACTGTT 
TATAAGGGAC TTTGGATCCC AGAAGGGGAA AAGGTTAAAA TTCCTGTTGC TATTAAAGAA 
TTGAGAGAGG CTACATCGCC AAAAGCCAAC AAGGAAATAC TTGATGAAGC CTATGTGATG 
GCTAGTGTTG ACAATCCTCA TGTGTGCCGC TTGCTGGGAA TCTGCCTCAC TTCCACTGTG 
CAGCTCATCA CCCAGCTTAT GCCTTATGGC TGCCTCCTTG ACTACATCCG AGAGCACAAG 
GACAACATTG GCTCCCAGTA CCTTCTCAAC TGGTGTGTGC AGATTGCAAA GGGAATGAAC 
TACCTGGAGG AACGTCGCCT GGTGCACCGT GACCTTGCTG CCAGGAACGT CCTTGTTAAG 
ACTCCACAAC ATGTGAAAAT CACAGACTTT GGGCTGGCAA AGCTGCTTGG GGCAGATGAG 
AAGGAGTATC ACGCAGAGGG AGGCAAGGTT CCTATTAAAT GGATGGCATT GGAGTCAATT 
TTACACCGAA TTTATACTCA TCAAAGTGAT GTCTGGAGTT ATGGTGTGAC AGTTTGGGAG 
TTGATGACAT TTGGGTCCAA GCCTTATGAC GGGATCCCCG CAAGTGAAAT CTCCTCCGTC 
TTGGAGAAGG GAGAGCGTTT GCCCCAGCCA CCCATTTGTA CCATTGATGT GTACATGATC 
ATGGTCAAAT GCTGGATGAT TGATGCAGAC AGCCGTCCCA AGTTTCGTGA GCTGATTGCA 
GAGTTCTCCA AAATGGCTCG TGACCCTCCC CGCTATCTTG TTATACAGGG AGATGAAAGG 
ATGCACTTGC CTAGCCCTAC AGATTCCAAG TTTTATCGCA CCCTGATGGA GGAGGAGGAC 
ATGGAAGACA TTGTGGATGC AGATGAGTAT CTTGTCCCAC ACCAGGGCTT TTTCAACAGC 
CCCTCTACAT CTCGGACTCC TCTTCTGAGT TCATTGAGCG CTACTAGCAA CAATTCTGCT 
ACAAACTGCA TTGACAGAAA TGGGCAGGGG CACCCTGTGA GGGAAGACAG CTTTGTCCAG 
AGGTACAGCT CAGATCCAAC AGGAAACTTC TTGGAGGAGA GTATAGATGA TGGCTTCCTG 
CCTGCTCCAG AGTATGTAAA CCAGCTGATG CCCAAGAAAC CATCTACTGC CATGGTCCAG 
AATCAAATCT ACAACAACAT CTCTCTCACA GCAATCTCAA AGCTCCCCAT GGACTCAAGA 
TACCAGAATT CCCACAGCAC AGCTGTGGAC AACCCTGAGT ATCTCAACAC TAACCAGTCC 
CCACTGGCCA AAACAGTCTT CGAGAGCTCT CCCTATTGGA TCCAATCAGG CAATCACCAA 
ATAAATCTGG ACAATCCTGA CTACCAGCAG GACTTTTTAC CAAATGAAAC AAAACCTAAT 
GGTCTCTTGA AAGTTCCTGC AGCAGAAAAC CCAGAGTACT TGAGGGTAGC AGCACCAAAA 
AGTGAATACA TTGAAGCTTC AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGFR ( XM_015281330.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015136816.1 Gallus gallus epidermal growth factor receptor (EGFR), transcript variant X3, mRNA.

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CloneID	OGa31514
Clone ID Related Accession (Same CDS sequence)	XM_015281330.1
Accession Version	XM_015281330.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	epidermal growth factor receptor isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA GAGGAGCCTG GATCCTTGAG CCTTGTCCAA AATGCCATCC GAACTGCACA 
GAAGACCACT GCTGGGGTGC TGGTGAACAG AACTGCCAGA CTTTAACAAA AGTCATCTGT 
GCCCAGCAAT GCTCTGGCCG GTGCAGAGGA AAGGTGCCCA GTGACTGCTG CCACAATCAG 
TGTGCTGCAG GGTGCACAGG ACCTCGGGAG AGTGACTGCC TGGCATGCCG CAAGTTTCGG 
GATGATGCTA CCTGCAAGGA CACATGTCCC CCACTGGTCC TCTATAACCC CACCACCTAT 
CAAATGGATG TCAACCCCGA GGGAAAATAC AGCTTTGGAG CCACTTGTGT GAGGGAATGT 
CCACACAACT ATGTGGTGAC AGATCATGGC TCCTGCGTTC GCTCGTGTAA TACTGATACT 
TACGAAGTGG AAGAAAATGG TGTTCGGAAG TGTAAAAAAT GTGATGGGCT ATGTAGCAAA 
GTGTGCAATG GCATTGGAAT AGGTGAACTT AAAGGGATCC TATCCATAAA TGCCACAAAC 
ATCGACTCCT TCAAAAACTG TACGAAGATC AATGGGGATG TCAGCATTCT TCCTGTTGCA 
TTTCTAGGGG ATGCCTTCAC AAAGACACTA CCCCTTGACC CTAAGAAGCT GGATGTCTTT 
AGAACAGTCA AAGAAATATC AGGATTTTTG TTGATTCAGG CCTGGCCTGA TAATGCTACT 
GATCTCTATG CTTTTGAAAA TCTGGAGATT ATCCGAGGCC GAACCAAGCA GCACGGCCAG 
TATTCCCTTG CTGTTGTTAA CTTGAAAATA CAGTCGTTGG GGCTGCGCTC CCTCAAGGAA 
ATAAGTGATG GAGACATTGC CATTATGAAG AACAAGAACC TCTGCTATGC TGACACCATG 
AACTGGCGCA GCTTGTTTGC TACTCAGAGT CAGAAAACAA AAATTATACA GAACAGAAAT 
AAAAATGATT GTACTGCTGA CAGGCATGTG TGTGACCCGC TGTGCTCGGA CGTGGGCTGC 
TGGGGCCCAG GGCCCTTCCA CTGCTTTTCC TGCAGGTTTT TCAGTCGCCA GAAGGAGTGT 
GTAAAACAGT GCAACATCCT GCAAGGGGAG CCACGTGAGT TTGAAAGAGA CTCCAAATGC 
CTCCCCTGCC ACTCAGAGTG TCTGGTACAG AACTCCACTG CATACAACAC AACCTGCTCT 
GGACCGGGCC CAGACCACTG CATGAAGTGT GCCCATTTTA TAGATGGTCC CCACTGTGTG 
AAGGCCTGCC CCGCTGGGGT CCTGGGTGAG AATGATACCC TGGTCTGGAA GTATGCAGAT 
GCCAATGCTG TTTGCCAGCT CTGCCATCCA AACTGTACAC GAGGGTGCAA AGGACCAGGT 
CTTGAAGGAT GTCCAAATGG CTCCAAAACT CCATCTATCG CGGCTGGTGT TGTCGGAGGA 
CTCCTGTGCC TGGTTGTGGT TGGTCTAGGC ATCGGTCTTT ACCTGCGGCG ACGTCATATC 
GTGCGGAAGC GCACCCTGCG CAGGCTGCTG CAAGAGAGGG AGCTTGTCGA ACCACTGACA 
CCCAGTGGGG AGGCACCAAA CCAGGCCCAC CTGAGAATTT TAAAGGAAAC AGAATTTAAA 
AAGGTCAAAG TTTTGGGCTC TGGAGCTTTT GGCACTGTTT ATAAGGGACT TTGGATCCCA 
GAAGGGGAAA AGGTTAAAAT TCCTGTTGCT ATTAAAGAAT TGAGAGAGGC TACATCGCCA 
AAAGCCAACA AGGAAATACT TGATGAAGCC TATGTGATGG CTAGTGTTGA CAATCCTCAT 
GTGTGCCGCT TGCTGGGAAT CTGCCTCACT TCCACTGTGC AGCTCATCAC CCAGCTTATG 
CCTTATGGCT GCCTCCTTGA CTACATCCGA GAGCACAAGG ACAACATTGG CTCCCAGTAC 
CTTCTCAACT GGTGTGTGCA GATTGCAAAG GGAATGAACT ACCTGGAGGA ACGTCGCCTG 
GTGCACCGTG ACCTTGCTGC CAGGAACGTC CTTGTTAAGA CTCCACAACA TGTGAAAATC 
ACAGACTTTG GGCTGGCAAA GCTGCTTGGG GCAGATGAGA AGGAGTATCA CGCAGAGGGA 
GGCAAGGTTC CTATTAAATG GATGGCATTG GAGTCAATTT TACACCGAAT TTATACTCAT 
CAAAGTGATG TCTGGAGTTA TGGTGTGACA GTTTGGGAGT TGATGACATT TGGGTCCAAG 
CCTTATGACG GGATCCCCGC AAGTGAAATC TCCTCCGTCT TGGAGAAGGG AGAGCGTTTG 
CCCCAGCCAC CCATTTGTAC CATTGATGTG TACATGATCA TGGTCAAATG CTGGATGATT 
GATGCAGACA GCCGTCCCAA GTTTCGTGAG CTGATTGCAG AGTTCTCCAA AATGGCTCGT 
GACCCTCCCC GCTATCTTGT TATACAGGGA GATGAAAGGA TGCACTTGCC TAGCCCTACA 
GATTCCAAGT TTTATCGCAC CCTGATGGAG GAGGAGGACA TGGAAGACAT TGTGGATGCA 
GATGAGTATC TTGTCCCACA CCAGGGCTTT TTCAACAGCC CCTCTACATC TCGGACTCCT 
CTTCTGAGTT CATTGAGCGC TACTAGCAAC AATTCTGCTA CAAACTGCAT TGACAGAAAT 
GGGCAGGGGC ACCCTGTGAG GGAAGACAGC TTTGTCCAGA GGTACAGCTC AGATCCAACA 
GGAAACTTCT TGGAGGAGAG TATAGATGAT GGCTTCCTGC CTGCTCCAGA GTATGTAAAC 
CAGCTGATGC CCAAGAAACC ATCTACTGCC ATGGTCCAGA ATCAAATCTA CAACAACATC 
TCTCTCACAG CAATCTCAAA GCTCCCCATG GACTCAAGAT ACCAGAATTC CCACAGCACA 
GCTGTGGACA ACCCTGAGTA TCTCAACACT AACCAGTCCC CACTGGCCAA AACAGTCTTC 
GAGAGCTCTC CCTATTGGAT CCAATCAGGC AATCACCAAA TAAATCTGGA CAATCCTGAC 
TACCAGCAGG ACTTTTTACC AAATGAAACA AAACCTAATG GTCTCTTGAA AGTTCCTGCA 
GCAGAAAACC CAGAGTACTT GAGGGTAGCA GCACCAAAAA GTGAATACAT TGAAGCTTCA 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015136816.1
CDS	31..3156
Translation	MSLRGAWILEPCPKCHPNCTEDHCWGAGEQNCQTLTKVICAQQCSGRCRGKVPSDCCHNQCAAGCTGPRESDCLACRKFRDDATCKDTCPPLVLYNPTTYQMDVNPEGKYSFGATCVRECPHNYVVTDHGSCVRSCNTDTYEVEENGVRKCKKCDGLCSKVCNGIGIGELKGILSINATNIDSFKNCTKINGDVSILPVAFLGDAFTKTLPLDPKKLDVFRTVKEISGFLLIQAWPDNATDLYAFENLEIIRGRTKQHGQYSLAVVNLKIQSLGLRSLKEISDGDIAIMKNKNLCYADTMNWRSLFATQSQKTKIIQNRNKNDCTADRHVCDPLCSDVGCWGPGPFHCFSCRFFSRQKECVKQCNILQGEPREFERDSKCLPCHSECLVQNSTAYNTTCSGPGPDHCMKCAHFIDGPHCVKACPAGVLGENDTLVWKYADANAVCQLCHPNCTRGCKGPGLEGCPNGSKTPSIAAGVVGGLLCLVVVGLGIGLYLRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQAHLRILKETEFKKVKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPYGCLLDYIREHKDNIGSQYLLNWCVQIAKGMNYLEERRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGADEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSVLEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIAEFSKMARDPPRYLVIQGDERMHLPSPTDSKFYRTLMEEEDMEDIVDADEYLVPHQGFFNSPSTSRTPLLSSLSATSNNSATNCIDRNGQGHPVREDSFVQRYSSDPTGNFLEESIDDGFLPAPEYVNQLMPKKPSTAMVQNQIYNNISLTAISKLPMDSRYQNSHSTAVDNPEYLNTNQSPLAKTVFESSPYWIQSGNHQINLDNPDYQQDFLPNETKPNGLLKVPAAENPEYLRVAAPKSEYIEASA

Target ORF information:

RefSeq Version	XM_015281330.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus epidermal growth factor receptor (EGFR), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281330.1

ORF Insert Sequence:

ATGTCACTGA GAGGAGCCTG GATCCTTGAG CCTTGTCCAA AATGCCATCC GAACTGCACA 
GAAGACCACT GCTGGGGTGC TGGTGAACAG AACTGCCAGA CTTTAACAAA AGTCATCTGT 
GCCCAGCAAT GCTCTGGCCG GTGCAGAGGA AAGGTGCCCA GTGACTGCTG CCACAATCAG 
TGTGCTGCAG GGTGCACAGG ACCTCGGGAG AGTGACTGCC TGGCATGCCG CAAGTTTCGG 
GATGATGCTA CCTGCAAGGA CACATGTCCC CCACTGGTCC TCTATAACCC CACCACCTAT 
CAAATGGATG TCAACCCCGA GGGAAAATAC AGCTTTGGAG CCACTTGTGT GAGGGAATGT 
CCACACAACT ATGTGGTGAC AGATCATGGC TCCTGCGTTC GCTCGTGTAA TACTGATACT 
TACGAAGTGG AAGAAAATGG TGTTCGGAAG TGTAAAAAAT GTGATGGGCT ATGTAGCAAA 
GTGTGCAATG GCATTGGAAT AGGTGAACTT AAAGGGATCC TATCCATAAA TGCCACAAAC 
ATCGACTCCT TCAAAAACTG TACGAAGATC AATGGGGATG TCAGCATTCT TCCTGTTGCA 
TTTCTAGGGG ATGCCTTCAC AAAGACACTA CCCCTTGACC CTAAGAAGCT GGATGTCTTT 
AGAACAGTCA AAGAAATATC AGGATTTTTG TTGATTCAGG CCTGGCCTGA TAATGCTACT 
GATCTCTATG CTTTTGAAAA TCTGGAGATT ATCCGAGGCC GAACCAAGCA GCACGGCCAG 
TATTCCCTTG CTGTTGTTAA CTTGAAAATA CAGTCGTTGG GGCTGCGCTC CCTCAAGGAA 
ATAAGTGATG GAGACATTGC CATTATGAAG AACAAGAACC TCTGCTATGC TGACACCATG 
AACTGGCGCA GCTTGTTTGC TACTCAGAGT CAGAAAACAA AAATTATACA GAACAGAAAT 
AAAAATGATT GTACTGCTGA CAGGCATGTG TGTGACCCGC TGTGCTCGGA CGTGGGCTGC 
TGGGGCCCAG GGCCCTTCCA CTGCTTTTCC TGCAGGTTTT TCAGTCGCCA GAAGGAGTGT 
GTAAAACAGT GCAACATCCT GCAAGGGGAG CCACGTGAGT TTGAAAGAGA CTCCAAATGC 
CTCCCCTGCC ACTCAGAGTG TCTGGTACAG AACTCCACTG CATACAACAC AACCTGCTCT 
GGACCGGGCC CAGACCACTG CATGAAGTGT GCCCATTTTA TAGATGGTCC CCACTGTGTG 
AAGGCCTGCC CCGCTGGGGT CCTGGGTGAG AATGATACCC TGGTCTGGAA GTATGCAGAT 
GCCAATGCTG TTTGCCAGCT CTGCCATCCA AACTGTACAC GAGGGTGCAA AGGACCAGGT 
CTTGAAGGAT GTCCAAATGG CTCCAAAACT CCATCTATCG CGGCTGGTGT TGTCGGAGGA 
CTCCTGTGCC TGGTTGTGGT TGGTCTAGGC ATCGGTCTTT ACCTGCGGCG ACGTCATATC 
GTGCGGAAGC GCACCCTGCG CAGGCTGCTG CAAGAGAGGG AGCTTGTCGA ACCACTGACA 
CCCAGTGGGG AGGCACCAAA CCAGGCCCAC CTGAGAATTT TAAAGGAAAC AGAATTTAAA 
AAGGTCAAAG TTTTGGGCTC TGGAGCTTTT GGCACTGTTT ATAAGGGACT TTGGATCCCA 
GAAGGGGAAA AGGTTAAAAT TCCTGTTGCT ATTAAAGAAT TGAGAGAGGC TACATCGCCA 
AAAGCCAACA AGGAAATACT TGATGAAGCC TATGTGATGG CTAGTGTTGA CAATCCTCAT 
GTGTGCCGCT TGCTGGGAAT CTGCCTCACT TCCACTGTGC AGCTCATCAC CCAGCTTATG 
CCTTATGGCT GCCTCCTTGA CTACATCCGA GAGCACAAGG ACAACATTGG CTCCCAGTAC 
CTTCTCAACT GGTGTGTGCA GATTGCAAAG GGAATGAACT ACCTGGAGGA ACGTCGCCTG 
GTGCACCGTG ACCTTGCTGC CAGGAACGTC CTTGTTAAGA CTCCACAACA TGTGAAAATC 
ACAGACTTTG GGCTGGCAAA GCTGCTTGGG GCAGATGAGA AGGAGTATCA CGCAGAGGGA 
GGCAAGGTTC CTATTAAATG GATGGCATTG GAGTCAATTT TACACCGAAT TTATACTCAT 
CAAAGTGATG TCTGGAGTTA TGGTGTGACA GTTTGGGAGT TGATGACATT TGGGTCCAAG 
CCTTATGACG GGATCCCCGC AAGTGAAATC TCCTCCGTCT TGGAGAAGGG AGAGCGTTTG 
CCCCAGCCAC CCATTTGTAC CATTGATGTG TACATGATCA TGGTCAAATG CTGGATGATT 
GATGCAGACA GCCGTCCCAA GTTTCGTGAG CTGATTGCAG AGTTCTCCAA AATGGCTCGT 
GACCCTCCCC GCTATCTTGT TATACAGGGA GATGAAAGGA TGCACTTGCC TAGCCCTACA 
GATTCCAAGT TTTATCGCAC CCTGATGGAG GAGGAGGACA TGGAAGACAT TGTGGATGCA 
GATGAGTATC TTGTCCCACA CCAGGGCTTT TTCAACAGCC CCTCTACATC TCGGACTCCT 
CTTCTGAGTT CATTGAGCGC TACTAGCAAC AATTCTGCTA CAAACTGCAT TGACAGAAAT 
GGGCAGGGGC ACCCTGTGAG GGAAGACAGC TTTGTCCAGA GGTACAGCTC AGATCCAACA 
GGAAACTTCT TGGAGGAGAG TATAGATGAT GGCTTCCTGC CTGCTCCAGA GTATGTAAAC 
CAGCTGATGC CCAAGAAACC ATCTACTGCC ATGGTCCAGA ATCAAATCTA CAACAACATC 
TCTCTCACAG CAATCTCAAA GCTCCCCATG GACTCAAGAT ACCAGAATTC CCACAGCACA 
GCTGTGGACA ACCCTGAGTA TCTCAACACT AACCAGTCCC CACTGGCCAA AACAGTCTTC 
GAGAGCTCTC CCTATTGGAT CCAATCAGGC AATCACCAAA TAAATCTGGA CAATCCTGAC 
TACCAGCAGG ACTTTTTACC AAATGAAACA AAACCTAATG GTCTCTTGAA AGTTCCTGCA 
GCAGAAAACC CAGAGTACTT GAGGGTAGCA GCACCAAAAA GTGAATACAT TGAAGCTTCA 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGFR ( NM_205497.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990828.2 Gallus gallus epidermal growth factor receptor (EGFR), mRNA.

Price & Availability↑

CloneID	OGa00162
Clone ID Related Accession (Same CDS sequence)	NM_205497.2
Accession Version	NM_205497.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Gallus gallus(chicken)
Product	epidermal growth factor receptor precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000034.1, M20386.1, M10066.1 and BU258290.1. On Oct 13, 2007 this sequence version replaced NM_205497.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

ATGGGTGTAC GCAGCCCCCT GTCCGCCTCT GGGCCTCGCG GGGCCGCTGT CCTGGTGCTA 
CTGCTGCTGC TGCTGGGGCG CGTCGCGCTG TGTTCCGCCG TGGAGGAGAA GAAAGTTTGT 
CAAGGGACAA ATAACAAGTT GACCCAGCTG GGGCACGTGG AAGACCATTT CACCAGCCTG 
CAGAGAATGT ACAACAACTG CGAAGTGGTA CTGAGTAACC TGGAGATTAC CTACGTGGAG 
CATAATCGCG ATCTCACCTT CCTTAAGACC ATACAGGAGG TTGCAGGCTA TGTGCTCATT 
GCGCTTAACA TGGTGGACGT CATTCCCTTA GAAAACCTCC AGATTATCCG AGGGAATGTG 
CTTTATGACA ACTCTTTTGC CCTGGCAGTT TTATCCAATT ACCACATGAA TAAAACCCAG 
GGACTTCGAG AGCTGCCAAT GAAACGGCTA TCAGAAATTC TCAATGGAGG TGTTAAAATC 
AGCAACAACC CCAAACTGTG CAACATGGAC ACTGTTCTCT GGAATGACAT CATTGATACA 
AGCAGGAAGC CTCTCACAGT ACTTGACTTT GCAAGCAATC TTTCTTCTTG TCCAAAATGC 
CATCCGAACT GCACAGAAGA CCACTGCTGG GGTGCTGGTG AACAGAACTG CCAGACTTTA 
ACAAAAGTCA TCTGTGCCCA GCAATGCTCT GGCCGGTGCA GAGGAAAGGT GCCCAGTGAC 
TGCTGCCACA ATCAGTGTGC TGCAGGGTGC ACAGGACCTC GGGAGAGTGA CTGCCTGGCA 
TGCCGCAAGT TTCGGGATGA TGCTACCTGC AAGGACACAT GTCCCCCACT GGTCCTCTAT 
AACCCCACCA CCTATCAAAT GGATGTCAAC CCCGAGGGAA AATACAGCTT TGGAGCCACT 
TGTGTGAGGG AATGTCCACA CAACTATGTG GTGACAGATC ATGGCTCCTG CGTTCGCTCG 
TGTAATACTG ATACTTACGA AGTGGAAGAA AATGGTGTTC GGAAGTGTAA AAAATGTGAT 
GGGCTATGTA GCAAAGTGTG CAATGGCATT GGAATAGGTG AACTTAAAGG GATCCTATCC 
ATAAATGCCA CAAACATCGA CTCCTTCAAA AACTGTACGA AGATCAATGG GGATGTCAGC 
ATTCTTCCTG TTGCATTTCT AGGGGATGCC TTCACAAAGA CACTACCCCT TGACCCTAAG 
AAGCTGGATG TCTTTAGAAC AGTCAAAGAA ATATCAGGAT TTTTGTTGAT TCAGGCCTGG 
CCTGATAATG CTACTGATCT CTATGCTTTT GAAAATCTGG AGATTATCCG AGGCCGAACC 
AAGCAGCACG GCCAGTATTC CCTTGCTGTT GTTAACTTGA AAATACAGTC GTTGGGGCTG 
CGCTCCCTCA AGGAAATAAG TGATGGAGAC ATTGCCATTA TGAAGAACAA GAACCTCTGC 
TATGCTGACA CCATGAACTG GCGCAGCTTG TTTGCTACTC AGAGTCAGAA AACAAAAATT 
ATACAGAACA GAAATAAAAA TGATTGTACT GCTGACAGGC ATGTGTGTGA CCCGCTGTGC 
TCGGACGTGG GCTGCTGGGG CCCAGGGCCC TTCCACTGCT TTTCCTGCAG GTTTTTCAGT 
CGCCAGAAGG AGTGTGTAAA ACAGTGCAAC ATCCTGCAAG GGGAGCCACG TGAGTTTGAA 
AGAGACTCCA AATGCCTCCC CTGCCACTCA GAGTGTCTGG TACAGAACTC CACTGCATAC 
AACACAACCT GCTCTGGACC GGGCCCAGAC CACTGCATGA AGTGTGCCCA TTTTATAGAT 
GGTCCCCACT GTGTGAAGGC CTGCCCCGCT GGGGTCCTGG GTGAGAATGA TACCCTGGTC 
TGGAAGTATG CAGATGCCAA TGCTGTTTGC CAGCTCTGCC ATCCAAACTG TACACGAGGG 
TGCAAAGGAC CAGGTCTTGA AGGATGTCCA AATGGCTCCA AAACTCCATC TATCGCGGCT 
GGTGTTGTCG GAGGACTCCT GTGCCTGGTT GTGGTTGGTC TAGGCATCGG TCTTTACCTG 
CGGCGACGTC ATATCGTGCG GAAGCGCACC CTGCGCAGGC TGCTGCAAGA GAGGGAGCTT 
GTCGAACCAC TGACACCCAG TGGGGAGGCA CCAAACCAGG CCCACCTGAG AATTTTAAAG 
GAAACAGAAT TTAAAAAGGT CAAAGTTTTG GGCTCTGGAG CTTTTGGCAC TGTTTATAAG 
GGACTTTGGA TCCCAGAAGG GGAAAAGGTT AAAATTCCTG TTGCTATTAA AGAATTGAGA 
GAGGCTACAT CGCCAAAAGC CAACAAGGAA ATACTTGATG AAGCCTATGT GATGGCTAGT 
GTTGACAATC CTCATGTGTG CCGCTTGCTG GGAATCTGCC TCACTTCCAC TGTGCAGCTC 
ATCACCCAGC TTATGCCTTA TGGCTGCCTC CTTGACTACA TCCGAGAGCA CAAGGACAAC 
ATTGGCTCCC AGTACCTTCT CAACTGGTGT GTGCAGATTG CAAAGGGAAT GAACTACCTG 
GAGGAACGTC GCCTGGTGCA CCGTGACCTT GCTGCCAGGA ACGTCCTTGT TAAGACTCCA 
CAACATGTGA AAATCACAGA CTTTGGGCTG GCAAAGCTGC TTGGGGCAGA TGAGAAGGAG 
TATCACGCAG AGGGAGGCAA GGTTCCTATT AAATGGATGG CATTGGAGTC AATTTTACAC 
CGAATTTATA CTCATCAAAG TGATGTCTGG AGTTATGGTG TGACAGTTTG GGAGTTGATG 
ACATTTGGGT CCAAGCCTTA TGACGGGATC CCCGCAAGTG AAATCTCCTC CGTCTTGGAG 
AAGGGAGAGC GTTTGCCCCA GCCACCCATT TGTACCATTG ATGTGTACAT GATCATGGTC 
AAATGCTGGA TGATTGATGC AGACAGCCGT CCCAAGTTTC GTGAGCTGAT TGCAGAGTTC 
TCCAAAATGG CTCGTGACCC TCCCCGCTAT CTTGTTATAC AGGGAGATGA AAGGATGCAC 
TTGCCTAGCC CTACAGATTC CAAGTTTTAT CGCACCCTGA TGGAGGAGGA GGACATGGAA 
GACATTGTGG ATGCAGATGA GTATCTTGTC CCACACCAGG GCTTTTTCAA CAGCCCCTCT 
ACATCTCGGA CTCCTCTTCT GAGTTCATTG AGCGCTACTA GCAACAATTC TGCTACAAAC 
TGCATTGACA GAAATGGGCA GGGGCACCCT GTGAGGGAAG ACAGCTTTGT CCAGAGGTAC 
AGCTCAGATC CAACAGGAAA CTTCTTGGAG GAGAGTATAG ATGATGGCTT CCTGCCTGCT 
CCAGAGTATG TAAACCAGCT GATGCCCAAG AAACCATCTA CTGCCATGGT CCAGAATCAA 
ATCTACAACA ACATCTCTCT CACAGCAATC TCAAAGCTCC CCATGGACTC AAGATACCAG 
AATTCCCACA GCACAGCTGT GGACAACCCT GAGTATCTCA ACACTAACCA GTCCCCACTG 
GCCAAAACAG TCTTCGAGAG CTCTCCCTAT TGGATCCAAT CAGGCAATCA CCAAATAAAT 
CTGGACAATC CTGACTACCA GCAGGACTTT TTACCAAATG AAACAAAACC TAATGGTCTC 
TTGAAAGTTC CTGCAGCAGA AAACCCAGAG TACTTGAGGG TAGCAGCACC AAAAAGTGAA 
TACATTGAAG CTTCAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990828.2
CDS	299..3979
Translation	MGVRSPLSASGPRGAAVLVLLLLLLGRVALCSAVEEKKVCQGTNNKLTQLGHVEDHFTSLQRMYNNCEVVLSNLEITYVEHNRDLTFLKTIQEVAGYVLIALNMVDVIPLENLQIIRGNVLYDNSFALAVLSNYHMNKTQGLRELPMKRLSEILNGGVKISNNPKLCNMDTVLWNDIIDTSRKPLTVLDFASNLSSCPKCHPNCTEDHCWGAGEQNCQTLTKVICAQQCSGRCRGKVPSDCCHNQCAAGCTGPRESDCLACRKFRDDATCKDTCPPLVLYNPTTYQMDVNPEGKYSFGATCVRECPHNYVVTDHGSCVRSCNTDTYEVEENGVRKCKKCDGLCSKVCNGIGIGELKGILSINATNIDSFKNCTKINGDVSILPVAFLGDAFTKTLPLDPKKLDVFRTVKEISGFLLIQAWPDNATDLYAFENLEIIRGRTKQHGQYSLAVVNLKIQSLGLRSLKEISDGDIAIMKNKNLCYADTMNWRSLFATQSQKTKIIQNRNKNDCTADRHVCDPLCSDVGCWGPGPFHCFSCRFFSRQKECVKQCNILQGEPREFERDSKCLPCHSECLVQNSTAYNTTCSGPGPDHCMKCAHFIDGPHCVKACPAGVLGENDTLVWKYADANAVCQLCHPNCTRGCKGPGLEGCPNGSKTPSIAAGVVGGLLCLVVVGLGIGLYLRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQAHLRILKETEFKKVKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPYGCLLDYIREHKDNIGSQYLLNWCVQIAKGMNYLEERRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGADEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSVLEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIAEFSKMARDPPRYLVIQGDERMHLPSPTDSKFYRTLMEEEDMEDIVDADEYLVPHQGFFNSPSTSRTPLLSSLSATSNNSATNCIDRNGQGHPVREDSFVQRYSSDPTGNFLEESIDDGFLPAPEYVNQLMPKKPSTAMVQNQIYNNISLTAISKLPMDSRYQNSHSTAVDNPEYLNTNQSPLAKTVFESSPYWIQSGNHQINLDNPDYQQDFLPNETKPNGLLKVPAAENPEYLRVAAPKSEYIEASA

Target ORF information:

RefSeq Version	NM_205497.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus epidermal growth factor receptor (EGFR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205497.2

ORF Insert Sequence:

ATGGGTGTAC GCAGCCCCCT GTCCGCCTCT GGGCCTCGCG GGGCCGCTGT CCTGGTGCTA 
CTGCTGCTGC TGCTGGGGCG CGTCGCGCTG TGTTCCGCCG TGGAGGAGAA GAAAGTTTGT 
CAAGGGACAA ATAACAAGTT GACCCAGCTG GGGCACGTGG AAGACCATTT CACCAGCCTG 
CAGAGAATGT ACAACAACTG CGAAGTGGTA CTGAGTAACC TGGAGATTAC CTACGTGGAG 
CATAATCGCG ATCTCACCTT CCTTAAGACC ATACAGGAGG TTGCAGGCTA TGTGCTCATT 
GCGCTTAACA TGGTGGACGT CATTCCCTTA GAAAACCTCC AGATTATCCG AGGGAATGTG 
CTTTATGACA ACTCTTTTGC CCTGGCAGTT TTATCCAATT ACCACATGAA TAAAACCCAG 
GGACTTCGAG AGCTGCCAAT GAAACGGCTA TCAGAAATTC TCAATGGAGG TGTTAAAATC 
AGCAACAACC CCAAACTGTG CAACATGGAC ACTGTTCTCT GGAATGACAT CATTGATACA 
AGCAGGAAGC CTCTCACAGT ACTTGACTTT GCAAGCAATC TTTCTTCTTG TCCAAAATGC 
CATCCGAACT GCACAGAAGA CCACTGCTGG GGTGCTGGTG AACAGAACTG CCAGACTTTA 
ACAAAAGTCA TCTGTGCCCA GCAATGCTCT GGCCGGTGCA GAGGAAAGGT GCCCAGTGAC 
TGCTGCCACA ATCAGTGTGC TGCAGGGTGC ACAGGACCTC GGGAGAGTGA CTGCCTGGCA 
TGCCGCAAGT TTCGGGATGA TGCTACCTGC AAGGACACAT GTCCCCCACT GGTCCTCTAT 
AACCCCACCA CCTATCAAAT GGATGTCAAC CCCGAGGGAA AATACAGCTT TGGAGCCACT 
TGTGTGAGGG AATGTCCACA CAACTATGTG GTGACAGATC ATGGCTCCTG CGTTCGCTCG 
TGTAATACTG ATACTTACGA AGTGGAAGAA AATGGTGTTC GGAAGTGTAA AAAATGTGAT 
GGGCTATGTA GCAAAGTGTG CAATGGCATT GGAATAGGTG AACTTAAAGG GATCCTATCC 
ATAAATGCCA CAAACATCGA CTCCTTCAAA AACTGTACGA AGATCAATGG GGATGTCAGC 
ATTCTTCCTG TTGCATTTCT AGGGGATGCC TTCACAAAGA CACTACCCCT TGACCCTAAG 
AAGCTGGATG TCTTTAGAAC AGTCAAAGAA ATATCAGGAT TTTTGTTGAT TCAGGCCTGG 
CCTGATAATG CTACTGATCT CTATGCTTTT GAAAATCTGG AGATTATCCG AGGCCGAACC 
AAGCAGCACG GCCAGTATTC CCTTGCTGTT GTTAACTTGA AAATACAGTC GTTGGGGCTG 
CGCTCCCTCA AGGAAATAAG TGATGGAGAC ATTGCCATTA TGAAGAACAA GAACCTCTGC 
TATGCTGACA CCATGAACTG GCGCAGCTTG TTTGCTACTC AGAGTCAGAA AACAAAAATT 
ATACAGAACA GAAATAAAAA TGATTGTACT GCTGACAGGC ATGTGTGTGA CCCGCTGTGC 
TCGGACGTGG GCTGCTGGGG CCCAGGGCCC TTCCACTGCT TTTCCTGCAG GTTTTTCAGT 
CGCCAGAAGG AGTGTGTAAA ACAGTGCAAC ATCCTGCAAG GGGAGCCACG TGAGTTTGAA 
AGAGACTCCA AATGCCTCCC CTGCCACTCA GAGTGTCTGG TACAGAACTC CACTGCATAC 
AACACAACCT GCTCTGGACC GGGCCCAGAC CACTGCATGA AGTGTGCCCA TTTTATAGAT 
GGTCCCCACT GTGTGAAGGC CTGCCCCGCT GGGGTCCTGG GTGAGAATGA TACCCTGGTC 
TGGAAGTATG CAGATGCCAA TGCTGTTTGC CAGCTCTGCC ATCCAAACTG TACACGAGGG 
TGCAAAGGAC CAGGTCTTGA AGGATGTCCA AATGGCTCCA AAACTCCATC TATCGCGGCT 
GGTGTTGTCG GAGGACTCCT GTGCCTGGTT GTGGTTGGTC TAGGCATCGG TCTTTACCTG 
CGGCGACGTC ATATCGTGCG GAAGCGCACC CTGCGCAGGC TGCTGCAAGA GAGGGAGCTT 
GTCGAACCAC TGACACCCAG TGGGGAGGCA CCAAACCAGG CCCACCTGAG AATTTTAAAG 
GAAACAGAAT TTAAAAAGGT CAAAGTTTTG GGCTCTGGAG CTTTTGGCAC TGTTTATAAG 
GGACTTTGGA TCCCAGAAGG GGAAAAGGTT AAAATTCCTG TTGCTATTAA AGAATTGAGA 
GAGGCTACAT CGCCAAAAGC CAACAAGGAA ATACTTGATG AAGCCTATGT GATGGCTAGT 
GTTGACAATC CTCATGTGTG CCGCTTGCTG GGAATCTGCC TCACTTCCAC TGTGCAGCTC 
ATCACCCAGC TTATGCCTTA TGGCTGCCTC CTTGACTACA TCCGAGAGCA CAAGGACAAC 
ATTGGCTCCC AGTACCTTCT CAACTGGTGT GTGCAGATTG CAAAGGGAAT GAACTACCTG 
GAGGAACGTC GCCTGGTGCA CCGTGACCTT GCTGCCAGGA ACGTCCTTGT TAAGACTCCA 
CAACATGTGA AAATCACAGA CTTTGGGCTG GCAAAGCTGC TTGGGGCAGA TGAGAAGGAG 
TATCACGCAG AGGGAGGCAA GGTTCCTATT AAATGGATGG CATTGGAGTC AATTTTACAC 
CGAATTTATA CTCATCAAAG TGATGTCTGG AGTTATGGTG TGACAGTTTG GGAGTTGATG 
ACATTTGGGT CCAAGCCTTA TGACGGGATC CCCGCAAGTG AAATCTCCTC CGTCTTGGAG 
AAGGGAGAGC GTTTGCCCCA GCCACCCATT TGTACCATTG ATGTGTACAT GATCATGGTC 
AAATGCTGGA TGATTGATGC AGACAGCCGT CCCAAGTTTC GTGAGCTGAT TGCAGAGTTC 
TCCAAAATGG CTCGTGACCC TCCCCGCTAT CTTGTTATAC AGGGAGATGA AAGGATGCAC 
TTGCCTAGCC CTACAGATTC CAAGTTTTAT CGCACCCTGA TGGAGGAGGA GGACATGGAA 
GACATTGTGG ATGCAGATGA GTATCTTGTC CCACACCAGG GCTTTTTCAA CAGCCCCTCT 
ACATCTCGGA CTCCTCTTCT GAGTTCATTG AGCGCTACTA GCAACAATTC TGCTACAAAC 
TGCATTGACA GAAATGGGCA GGGGCACCCT GTGAGGGAAG ACAGCTTTGT CCAGAGGTAC 
AGCTCAGATC CAACAGGAAA CTTCTTGGAG GAGAGTATAG ATGATGGCTT CCTGCCTGCT 
CCAGAGTATG TAAACCAGCT GATGCCCAAG AAACCATCTA CTGCCATGGT CCAGAATCAA 
ATCTACAACA ACATCTCTCT CACAGCAATC TCAAAGCTCC CCATGGACTC AAGATACCAG 
AATTCCCACA GCACAGCTGT GGACAACCCT GAGTATCTCA ACACTAACCA GTCCCCACTG 
GCCAAAACAG TCTTCGAGAG CTCTCCCTAT TGGATCCAAT CAGGCAATCA CCAAATAAAT 
CTGGACAATC CTGACTACCA GCAGGACTTT TTACCAAATG AAACAAAACC TAATGGTCTC 
TTGAAAGTTC CTGCAGCAGA AAACCCAGAG TACTTGAGGG TAGCAGCACC AAAAAGTGAA 
TACATTGAAG CTTCAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Journal of virology91(20)(2017 Oct)
Pecenka V,Pajer P,Karafiat V,Kasparova P,Dudlova J,Dvorak M

DNA methylation down-regulates EGFR expression in chickens.
Avian diseases57(2 Suppl)366-71(2013 Jun)
Luo J,Chang S,Zhang H,Li B,Song J

EGFR signaling is required for regenerative proliferation in the cochlea: conservation in birds and mammals.
Developmental biology363(1)191-200(2012 Mar)
White PM,Stone JS,Groves AK,Segil N

Interleukin-6 promotes 2-deoxyglucose uptake through p44/42 MAPKs activation via Ca2+/PKC and EGF receptor in primary cultured chicken hepatocytes.
Journal of cellular physiology218(3)643-52(2009 Mar)
Suh HN,Lee YJ,Han HJ

Studies on epidermal growth factor receptor signaling in vertebrate limb patterning.
Developmental dynamics : an official publication of the American Association of Anatomists233(2)288-300(2005 Jun)
Omi M,Fisher M,Maihle NJ,Dealy CN

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EGFR cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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