NGF cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	NGF
Entrez Gene ID	396466
Full Name	nerve growth factor
Synonyms	NGFB,beta-NGF
General protein information	Preferred Names nerve growth factor Names beta-nerve growth factor nerve growth factor (beta polypeptide)
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015298897.2	XP_015154383.1	beta-nerve growth factor isoform X2
XM_015298897.1	XP_015154383.1	beta-nerve growth factor isoform X1
NM_001293108.1	NP_001280037.1	beta-nerve growth factor precursor
NM_001293109.1	NP_001280038.1	beta-nerve growth factor precursor
XM_025143679.1	XP_024999447.1	beta-nerve growth factor isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	ngf	NP_001123396.1
	LOC711681	XP_001100522.2
Mus musculus (house mouse)	Ngf	NP_038637.1
Pan troglodytes (chimpanzee)	NGF	NP_001012439.1
Homo sapiens (human)	NGF	NP_002497.2
Rattus norvegicus (Norway rat)	Ngf	NP_001263984.1
Canis lupus familiaris (dog)	NGF	NP_001181879.1
Bos taurus (cattle)	NGF	NP_001092832.1
Gallus gallus (chicken)	NGF	XP_004935065.1

Gene Ontology
Bibliography

Related articles in PubMed

Nerve growth factor promotes reorganization of the axonal microtubule array at sites of axon collateral branching.
Ketschek A, Jones S, Spillane M, Korobova F, Svitkina T, Gallo G
Developmental neurobiology75(12)1441-61(2015 Dec)

Nerve growth factor-induced formation of axonal filopodia and collateral branches involves the intra-axonal synthesis of regulators of the actin-nucleating Arp2/3 complex.
Spillane M, Ketschek A, Donnelly CJ, Pacheco A, Twiss JL, Gallo G
The Journal of neuroscience : the official journal of the Society for Neuroscience32(49)17671-89(2012 Dec)

Nerve growth factor regulates axial rotation during early stages of chick embryo development.
Manca A, Capsoni S, Di Luzio A, Vignone D, Malerba F, Paoletti F, Brandi R, Arisi I, Cattaneo A, Levi-Montalcini R
Proceedings of the National Academy of Sciences of the United States of America109(6)2009-14(2012 Feb)

Somatic tetraploidy in specific chick retinal ganglion cells induced by nerve growth factor.
Morillo SM, Escoll P, de la Hera A, Frade JM
Proceedings of the National Academy of Sciences of the United States of America107(1)109-14(2010 Jan)

[The expression level of NGF, BDNF and NT-3 in spinal cord of growing embryonic chicken].
Dai P, Xiyang YB
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition40(4)676-8(2009 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results suggest that NGF differentially affects the phosphorylation of MAP1B within the main axon shaft and the laterally projecting filopodia
Title: Nerve growth factor promotes reorganization of the axonal microtubule array at sites of axon collateral branching.

NGF promotes sensory axon branching through regulation of the actin cytoskeleton using both canonical signaling mechanisms and intra-axonal protein synthesis downstream of PI3K signaling.
Title: Nerve growth factor-induced formation of axonal filopodia and collateral branches involves the intra-axonal synthesis of regulators of the actin-nucleating Arp2/3 complex.

These results reveal a role for NGF in early chicken embryo development.
Title: Nerve growth factor regulates axial rotation during early stages of chick embryo development.

Data show that endogenous NGF induces DNA replication in a subpopulation of differentiating retinal ganglion cells that express both the neurotrophin receptor p75 and the E2F1 transcription factor, but that lack the retinoblastoma protein.
Title: Somatic tetraploidy in specific chick retinal ganglion cells induced by nerve growth factor.

Levels of NGF, BDNF and NT-3 were all up regulated from E7 to E14 in the spinal cord of embryonic chicken during development.
Title: [The expression level of NGF, BDNF and NT-3 in spinal cord of growing embryonic chicken].

The following NGF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NGF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa00656	NM_001293109.1 Latest version!	Gallus gallus nerve growth factor (NGF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OGa00656	NM_001293108.1 Latest version!	Gallus gallus nerve growth factor (NGF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OGa00656	XM_015298897.2 Latest version!	Gallus gallus nerve growth factor (NGF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OGa19826	XM_025143679.1 Latest version!	Gallus gallus nerve growth factor (NGF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
View Old Accession Versions >>
OGa00656	XM_015298897.1	Gallus gallus nerve growth factor (beta polypeptide) (NGF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NGF ( NM_001293108.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001280037.1 Gallus gallus nerve growth factor (NGF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OGa00656
Clone ID Related Accession (Same CDS sequence)	XM_015298897.2 , XM_015298897.1 , NM_001293108.1 , NM_001293109.1
Accession Version	NM_001293108.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Gallus gallus(chicken)
Product	beta-nerve growth factor precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CD217960.1, BU337330.1 and BU313448.1. On May 30, 2014 this sequence version replaced XM_003642729.2. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CD217960.1 [ECO:0000332] ##Evidence-Data-END##

ATGTCCATGC TGTACTACAC TCTGATTATA GCTTTTTTGA TCGGCACACA GGCAGCTCCA 
AAGTCAGAGG ACAATGGTCC GCTGGAGTAT CCTGCAGAAC ACTCCTTACC CAGTACACAA 
CAGAGTAACG GACAGCACAT TGCCAAGGCA GCCCCACAGA CAACCCACGG CCGCTTTGCT 
TGGATGCCAG ATGGAACAGA AGATTTAAAT ATCGCTATGG ACCAAAACTT CTTTAAGAAG 
AAACGTTTCC GGTCTTCTCG GGTCCTGTTC AGCACGCAGC CACCTCCAGT GTCAAGGAAA 
GGACAGAGTA CGGGGTTTCT CAGCAGTGCA GTCTCTCTCA ACAGGACTGC CAGGACCAAG 
AGGACTGCAC ATCCCGTACT GCACCGGGGA GAGTTCTCAG TGTGTGACAG TGTCAGCATG 
TGGGTCGGGG ACAAAACCAC CGCCACCGAC ATCAAAGGCA AAGAGGTGAC CGTGCTGGGA 
GAGGTCAACA TTAACAACAA CGTTTTTAAG CAGTACTTTT TCGAGACCAA GTGCAGGGAC 
CCTAGGCCGG TGTCCAGCGG GTGCCGAGGG ATCGATGCGA AGCATTGGAA CTCTTACTGC 
ACCACGACAC ACACCTTCGT CAAAGCACTG ACCATGGAGG GCAAGCAAGC AGCCTGGAGA 
TTTATCCGGA TCGACACAGC CTGTGTGTGT GTGCTCAGCA GGAAATCGGG GAGACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001280037.1
CDS	395..1114
Translation	MSMLYYTLIIAFLIGTQAAPKSEDNGPLEYPAEHSLPSTQQSNGQHIAKAAPQTTHGRFAWMPDGTEDLNIAMDQNFFKKKRFRSSRVLFSTQPPPVSRKGQSTGFLSSAVSLNRTARTKRTAHPVLHRGEFSVCDSVSMWVGDKTTATDIKGKEVTVLGEVNINNNVFKQYFFETKCRDPRPVSSGCRGIDAKHWNSYCTTTHTFVKALTMEGKQAAWRFIRIDTACVCVLSRKSGRP

Target ORF information:

RefSeq Version	NM_001293108.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nerve growth factor (NGF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001293108.1

ORF Insert Sequence:

ATGTCCATGC TGTACTACAC TCTGATTATA GCTTTTTTGA TCGGCACACA GGCAGCTCCA 
AAGTCAGAGG ACAATGGTCC GCTGGAGTAT CCTGCAGAAC ACTCCTTACC CAGTACACAA 
CAGAGTAACG GACAGCACAT TGCCAAGGCA GCCCCACAGA CAACCCACGG CCGCTTTGCT 
TGGATGCCAG ATGGAACAGA AGATTTAAAT ATCGCTATGG ACCAAAACTT CTTTAAGAAG 
AAACGTTTCC GGTCTTCTCG GGTCCTGTTC AGCACGCAGC CACCTCCAGT GTCAAGGAAA 
GGACAGAGTA CGGGGTTTCT CAGCAGTGCA GTCTCTCTCA ACAGGACTGC CAGGACCAAG 
AGGACTGCAC ATCCCGTACT GCACCGGGGA GAGTTCTCAG TGTGTGACAG TGTCAGCATG 
TGGGTCGGGG ACAAAACCAC CGCCACCGAC ATCAAAGGCA AAGAGGTGAC CGTGCTGGGA 
GAGGTCAACA TTAACAACAA CGTTTTTAAG CAGTACTTTT TCGAGACCAA GTGCAGGGAC 
CCTAGGCCGG TGTCCAGCGG GTGCCGAGGG ATCGATGCGA AGCATTGGAA CTCTTACTGC 
ACCACGACAC ACACCTTCGT CAAAGCACTG ACCATGGAGG GCAAGCAAGC AGCCTGGAGA 
TTTATCCGGA TCGACACAGC CTGTGTGTGT GTGCTCAGCA GGAAATCGGG GAGACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NGF ( NM_001293109.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001280038.1 Gallus gallus nerve growth factor (NGF), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OGa00656
Clone ID Related Accession (Same CDS sequence)	XM_015298897.2 , XM_015298897.1 , NM_001293108.1 , NM_001293109.1
Accession Version	NM_001293109.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Gallus gallus(chicken)
Product	beta-nerve growth factor precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BU382314.1, BU337330.1 and BU313448.1. On May 30, 2014 this sequence version replaced XM_418016.4. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU382314.1 [ECO:0000332] ##Evidence-Data-END##

ATGTCCATGC TGTACTACAC TCTGATTATA GCTTTTTTGA TCGGCACACA GGCAGCTCCA 
AAGTCAGAGG ACAATGGTCC GCTGGAGTAT CCTGCAGAAC ACTCCTTACC CAGTACACAA 
CAGAGTAACG GACAGCACAT TGCCAAGGCA GCCCCACAGA CAACCCACGG CCGCTTTGCT 
TGGATGCCAG ATGGAACAGA AGATTTAAAT ATCGCTATGG ACCAAAACTT CTTTAAGAAG 
AAACGTTTCC GGTCTTCTCG GGTCCTGTTC AGCACGCAGC CACCTCCAGT GTCAAGGAAA 
GGACAGAGTA CGGGGTTTCT CAGCAGTGCA GTCTCTCTCA ACAGGACTGC CAGGACCAAG 
AGGACTGCAC ATCCCGTACT GCACCGGGGA GAGTTCTCAG TGTGTGACAG TGTCAGCATG 
TGGGTCGGGG ACAAAACCAC CGCCACCGAC ATCAAAGGCA AAGAGGTGAC CGTGCTGGGA 
GAGGTCAACA TTAACAACAA CGTTTTTAAG CAGTACTTTT TCGAGACCAA GTGCAGGGAC 
CCTAGGCCGG TGTCCAGCGG GTGCCGAGGG ATCGATGCGA AGCATTGGAA CTCTTACTGC 
ACCACGACAC ACACCTTCGT CAAAGCACTG ACCATGGAGG GCAAGCAAGC AGCCTGGAGA 
TTTATCCGGA TCGACACAGC CTGTGTGTGT GTGCTCAGCA GGAAATCGGG GAGACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001280038.1
CDS	270..989
Translation	MSMLYYTLIIAFLIGTQAAPKSEDNGPLEYPAEHSLPSTQQSNGQHIAKAAPQTTHGRFAWMPDGTEDLNIAMDQNFFKKKRFRSSRVLFSTQPPPVSRKGQSTGFLSSAVSLNRTARTKRTAHPVLHRGEFSVCDSVSMWVGDKTTATDIKGKEVTVLGEVNINNNVFKQYFFETKCRDPRPVSSGCRGIDAKHWNSYCTTTHTFVKALTMEGKQAAWRFIRIDTACVCVLSRKSGRP

Target ORF information:

RefSeq Version	NM_001293109.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nerve growth factor (NGF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001293109.1

ORF Insert Sequence:

ATGTCCATGC TGTACTACAC TCTGATTATA GCTTTTTTGA TCGGCACACA GGCAGCTCCA 
AAGTCAGAGG ACAATGGTCC GCTGGAGTAT CCTGCAGAAC ACTCCTTACC CAGTACACAA 
CAGAGTAACG GACAGCACAT TGCCAAGGCA GCCCCACAGA CAACCCACGG CCGCTTTGCT 
TGGATGCCAG ATGGAACAGA AGATTTAAAT ATCGCTATGG ACCAAAACTT CTTTAAGAAG 
AAACGTTTCC GGTCTTCTCG GGTCCTGTTC AGCACGCAGC CACCTCCAGT GTCAAGGAAA 
GGACAGAGTA CGGGGTTTCT CAGCAGTGCA GTCTCTCTCA ACAGGACTGC CAGGACCAAG 
AGGACTGCAC ATCCCGTACT GCACCGGGGA GAGTTCTCAG TGTGTGACAG TGTCAGCATG 
TGGGTCGGGG ACAAAACCAC CGCCACCGAC ATCAAAGGCA AAGAGGTGAC CGTGCTGGGA 
GAGGTCAACA TTAACAACAA CGTTTTTAAG CAGTACTTTT TCGAGACCAA GTGCAGGGAC 
CCTAGGCCGG TGTCCAGCGG GTGCCGAGGG ATCGATGCGA AGCATTGGAA CTCTTACTGC 
ACCACGACAC ACACCTTCGT CAAAGCACTG ACCATGGAGG GCAAGCAAGC AGCCTGGAGA 
TTTATCCGGA TCGACACAGC CTGTGTGTGT GTGCTCAGCA GGAAATCGGG GAGACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NGF ( XM_015298897.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154383.1 Gallus gallus nerve growth factor (NGF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa00656
Clone ID Related Accession (Same CDS sequence)	XM_015298897.2 , XM_015298897.1 , NM_001293108.1 , NM_001293109.1
Accession Version	XM_015298897.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	beta-nerve growth factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298897.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCATGC TGTACTACAC TCTGATTATA GCTTTTTTGA TCGGCACACA GGCAGCTCCA 
AAGTCAGAGG ACAATGGTCC GCTGGAGTAT CCTGCAGAAC ACTCCTTACC CAGTACACAA 
CAGAGTAACG GACAGCACAT TGCCAAGGCA GCCCCACAGA CAACCCACGG CCGCTTTGCT 
TGGATGCCAG ATGGAACAGA AGATTTAAAT ATCGCTATGG ACCAAAACTT CTTTAAGAAG 
AAACGTTTCC GGTCTTCTCG GGTCCTGTTC AGCACGCAGC CACCTCCAGT GTCAAGGAAA 
GGACAGAGTA CGGGGTTTCT CAGCAGTGCA GTCTCTCTCA ACAGGACTGC CAGGACCAAG 
AGGACTGCAC ATCCCGTACT GCACCGGGGA GAGTTCTCAG TGTGTGACAG TGTCAGCATG 
TGGGTCGGGG ACAAAACCAC CGCCACCGAC ATCAAAGGCA AAGAGGTGAC CGTGCTGGGA 
GAGGTCAACA TTAACAACAA CGTTTTTAAG CAGTACTTTT TCGAGACCAA GTGCAGGGAC 
CCTAGGCCGG TGTCCAGCGG GTGCCGAGGG ATCGATGCGA AGCATTGGAA CTCTTACTGC 
ACCACGACAC ACACCTTCGT CAAAGCACTG ACCATGGAGG GCAAGCAAGC AGCCTGGAGA 
TTTATCCGGA TCGACACAGC CTGTGTGTGT GTGCTCAGCA GGAAATCGGG GAGACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154383.1
CDS	475..1194
Translation	MSMLYYTLIIAFLIGTQAAPKSEDNGPLEYPAEHSLPSTQQSNGQHIAKAAPQTTHGRFAWMPDGTEDLNIAMDQNFFKKKRFRSSRVLFSTQPPPVSRKGQSTGFLSSAVSLNRTARTKRTAHPVLHRGEFSVCDSVSMWVGDKTTATDIKGKEVTVLGEVNINNNVFKQYFFETKCRDPRPVSSGCRGIDAKHWNSYCTTTHTFVKALTMEGKQAAWRFIRIDTACVCVLSRKSGRP

Target ORF information:

RefSeq Version	XM_015298897.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nerve growth factor (NGF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298897.2

ORF Insert Sequence:

ATGTCCATGC TGTACTACAC TCTGATTATA GCTTTTTTGA TCGGCACACA GGCAGCTCCA 
AAGTCAGAGG ACAATGGTCC GCTGGAGTAT CCTGCAGAAC ACTCCTTACC CAGTACACAA 
CAGAGTAACG GACAGCACAT TGCCAAGGCA GCCCCACAGA CAACCCACGG CCGCTTTGCT 
TGGATGCCAG ATGGAACAGA AGATTTAAAT ATCGCTATGG ACCAAAACTT CTTTAAGAAG 
AAACGTTTCC GGTCTTCTCG GGTCCTGTTC AGCACGCAGC CACCTCCAGT GTCAAGGAAA 
GGACAGAGTA CGGGGTTTCT CAGCAGTGCA GTCTCTCTCA ACAGGACTGC CAGGACCAAG 
AGGACTGCAC ATCCCGTACT GCACCGGGGA GAGTTCTCAG TGTGTGACAG TGTCAGCATG 
TGGGTCGGGG ACAAAACCAC CGCCACCGAC ATCAAAGGCA AAGAGGTGAC CGTGCTGGGA 
GAGGTCAACA TTAACAACAA CGTTTTTAAG CAGTACTTTT TCGAGACCAA GTGCAGGGAC 
CCTAGGCCGG TGTCCAGCGG GTGCCGAGGG ATCGATGCGA AGCATTGGAA CTCTTACTGC 
ACCACGACAC ACACCTTCGT CAAAGCACTG ACCATGGAGG GCAAGCAAGC AGCCTGGAGA 
TTTATCCGGA TCGACACAGC CTGTGTGTGT GTGCTCAGCA GGAAATCGGG GAGACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NGF ( XM_015298897.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154383.1 Gallus gallus nerve growth factor (beta polypeptide) (NGF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa00656
Clone ID Related Accession (Same CDS sequence)	XM_015298897.2 , XM_015298897.1 , NM_001293108.1 , NM_001293109.1
Accession Version	XM_015298897.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	beta-nerve growth factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCATGC TGTACTACAC TCTGATTATA GCTTTTTTGA TCGGCACACA GGCAGCTCCA 
AAGTCAGAGG ACAATGGTCC GCTGGAGTAT CCTGCAGAAC ACTCCTTACC CAGTACACAA 
CAGAGTAACG GACAGCACAT TGCCAAGGCA GCCCCACAGA CAACCCACGG CCGCTTTGCT 
TGGATGCCAG ATGGAACAGA AGATTTAAAT ATCGCTATGG ACCAAAACTT CTTTAAGAAG 
AAACGTTTCC GGTCTTCTCG GGTCCTGTTC AGCACGCAGC CACCTCCAGT GTCAAGGAAA 
GGACAGAGTA CGGGGTTTCT CAGCAGTGCA GTCTCTCTCA ACAGGACTGC CAGGACCAAG 
AGGACTGCAC ATCCCGTACT GCACCGGGGA GAGTTCTCAG TGTGTGACAG TGTCAGCATG 
TGGGTCGGGG ACAAAACCAC CGCCACCGAC ATCAAAGGCA AAGAGGTGAC CGTGCTGGGA 
GAGGTCAACA TTAACAACAA CGTTTTTAAG CAGTACTTTT TCGAGACCAA GTGCAGGGAC 
CCTAGGCCGG TGTCCAGCGG GTGCCGAGGG ATCGATGCGA AGCATTGGAA CTCTTACTGC 
ACCACGACAC ACACCTTCGT CAAAGCACTG ACCATGGAGG GCAAGCAAGC AGCCTGGAGA 
TTTATCCGGA TCGACACAGC CTGTGTGTGT GTGCTCAGCA GGAAATCGGG GAGACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154383.1
CDS	709..1428
Translation	MSMLYYTLIIAFLIGTQAAPKSEDNGPLEYPAEHSLPSTQQSNGQHIAKAAPQTTHGRFAWMPDGTEDLNIAMDQNFFKKKRFRSSRVLFSTQPPPVSRKGQSTGFLSSAVSLNRTARTKRTAHPVLHRGEFSVCDSVSMWVGDKTTATDIKGKEVTVLGEVNINNNVFKQYFFETKCRDPRPVSSGCRGIDAKHWNSYCTTTHTFVKALTMEGKQAAWRFIRIDTACVCVLSRKSGRP

Target ORF information:

RefSeq Version	XM_015298897.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nerve growth factor (beta polypeptide) (NGF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298897.1

ORF Insert Sequence:

ATGTCCATGC TGTACTACAC TCTGATTATA GCTTTTTTGA TCGGCACACA GGCAGCTCCA 
AAGTCAGAGG ACAATGGTCC GCTGGAGTAT CCTGCAGAAC ACTCCTTACC CAGTACACAA 
CAGAGTAACG GACAGCACAT TGCCAAGGCA GCCCCACAGA CAACCCACGG CCGCTTTGCT 
TGGATGCCAG ATGGAACAGA AGATTTAAAT ATCGCTATGG ACCAAAACTT CTTTAAGAAG 
AAACGTTTCC GGTCTTCTCG GGTCCTGTTC AGCACGCAGC CACCTCCAGT GTCAAGGAAA 
GGACAGAGTA CGGGGTTTCT CAGCAGTGCA GTCTCTCTCA ACAGGACTGC CAGGACCAAG 
AGGACTGCAC ATCCCGTACT GCACCGGGGA GAGTTCTCAG TGTGTGACAG TGTCAGCATG 
TGGGTCGGGG ACAAAACCAC CGCCACCGAC ATCAAAGGCA AAGAGGTGAC CGTGCTGGGA 
GAGGTCAACA TTAACAACAA CGTTTTTAAG CAGTACTTTT TCGAGACCAA GTGCAGGGAC 
CCTAGGCCGG TGTCCAGCGG GTGCCGAGGG ATCGATGCGA AGCATTGGAA CTCTTACTGC 
ACCACGACAC ACACCTTCGT CAAAGCACTG ACCATGGAGG GCAAGCAAGC AGCCTGGAGA 
TTTATCCGGA TCGACACAGC CTGTGTGTGT GTGCTCAGCA GGAAATCGGG GAGACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NGF ( XM_025143679.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024999447.1 Gallus gallus nerve growth factor (NGF), transcript variant X1, mRNA.

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CloneID	OGa19826
Clone ID Related Accession (Same CDS sequence)	XM_025143679.1
Accession Version	XM_025143679.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	beta-nerve growth factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTGG GAAGGCAGAG GGAATGTGAA TGGCTCTTCT GGCTTGGGAA AGTATCCACA 
GACCGGGGTG CTACAGACTG CAAAACAGCC TGGACAGCCC TCCAGATTCC CGTCCCCTCT 
CAGGTGCATA GCGTAATGTC CATGCTGTAC TACACTCTGA TTATAGCTTT TTTGATCGGC 
ACACAGGCAG CTCCAAAGTC AGAGGACAAT GGTCCGCTGG AGTATCCTGC AGAACACTCC 
TTACCCAGTA CACAACAGAG TAACGGACAG CACATTGCCA AGGCAGCCCC ACAGACAACC 
CACGGCCGCT TTGCTTGGAT GCCAGATGGA ACAGAAGATT TAAATATCGC TATGGACCAA 
AACTTCTTTA AGAAGAAACG TTTCCGGTCT TCTCGGGTCC TGTTCAGCAC GCAGCCACCT 
CCAGTGTCAA GGAAAGGACA GAGTACGGGG TTTCTCAGCA GTGCAGTCTC TCTCAACAGG 
ACTGCCAGGA CCAAGAGGAC TGCACATCCC GTACTGCACC GGGGAGAGTT CTCAGTGTGT 
GACAGTGTCA GCATGTGGGT CGGGGACAAA ACCACCGCCA CCGACATCAA AGGCAAAGAG 
GTGACCGTGC TGGGAGAGGT CAACATTAAC AACAACGTTT TTAAGCAGTA CTTTTTCGAG 
ACCAAGTGCA GGGACCCTAG GCCGGTGTCC AGCGGGTGCC GAGGGATCGA TGCGAAGCAT 
TGGAACTCTT ACTGCACCAC GACACACACC TTCGTCAAAG CACTGACCAT GGAGGGCAAG 
CAAGCAGCCT GGAGATTTAT CCGGATCGAC ACAGCCTGTG TGTGTGTGCT CAGCAGGAAA 
TCGGGGAGAC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024999447.1
CDS	214..1068
Translation	MRLGRQRECEWLFWLGKVSTDRGATDCKTAWTALQIPVPSQVHSVMSMLYYTLIIAFLIGTQAAPKSEDNGPLEYPAEHSLPSTQQSNGQHIAKAAPQTTHGRFAWMPDGTEDLNIAMDQNFFKKKRFRSSRVLFSTQPPPVSRKGQSTGFLSSAVSLNRTARTKRTAHPVLHRGEFSVCDSVSMWVGDKTTATDIKGKEVTVLGEVNINNNVFKQYFFETKCRDPRPVSSGCRGIDAKHWNSYCTTTHTFVKALTMEGKQAAWRFIRIDTACVCVLSRKSGRP

Target ORF information:

RefSeq Version	XM_025143679.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nerve growth factor (NGF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025143679.1

ORF Insert Sequence:

ATGAGGCTGG GAAGGCAGAG GGAATGTGAA TGGCTCTTCT GGCTTGGGAA AGTATCCACA 
GACCGGGGTG CTACAGACTG CAAAACAGCC TGGACAGCCC TCCAGATTCC CGTCCCCTCT 
CAGGTGCATA GCGTAATGTC CATGCTGTAC TACACTCTGA TTATAGCTTT TTTGATCGGC 
ACACAGGCAG CTCCAAAGTC AGAGGACAAT GGTCCGCTGG AGTATCCTGC AGAACACTCC 
TTACCCAGTA CACAACAGAG TAACGGACAG CACATTGCCA AGGCAGCCCC ACAGACAACC 
CACGGCCGCT TTGCTTGGAT GCCAGATGGA ACAGAAGATT TAAATATCGC TATGGACCAA 
AACTTCTTTA AGAAGAAACG TTTCCGGTCT TCTCGGGTCC TGTTCAGCAC GCAGCCACCT 
CCAGTGTCAA GGAAAGGACA GAGTACGGGG TTTCTCAGCA GTGCAGTCTC TCTCAACAGG 
ACTGCCAGGA CCAAGAGGAC TGCACATCCC GTACTGCACC GGGGAGAGTT CTCAGTGTGT 
GACAGTGTCA GCATGTGGGT CGGGGACAAA ACCACCGCCA CCGACATCAA AGGCAAAGAG 
GTGACCGTGC TGGGAGAGGT CAACATTAAC AACAACGTTT TTAAGCAGTA CTTTTTCGAG 
ACCAAGTGCA GGGACCCTAG GCCGGTGTCC AGCGGGTGCC GAGGGATCGA TGCGAAGCAT 
TGGAACTCTT ACTGCACCAC GACACACACC TTCGTCAAAG CACTGACCAT GGAGGGCAAG 
CAAGCAGCCT GGAGATTTAT CCGGATCGAC ACAGCCTGTG TGTGTGTGCT CAGCAGGAAA 
TCGGGGAGAC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nerve growth factor promotes reorganization of the axonal microtubule array at sites of axon collateral branching.
Developmental neurobiology75(12)1441-61(2015 Dec)
Ketschek A,Jones S,Spillane M,Korobova F,Svitkina T,Gallo G

Nerve growth factor-induced formation of axonal filopodia and collateral branches involves the intra-axonal synthesis of regulators of the actin-nucleating Arp2/3 complex.
The Journal of neuroscience : the official journal of the Society for Neuroscience32(49)17671-89(2012 Dec)
Spillane M,Ketschek A,Donnelly CJ,Pacheco A,Twiss JL,Gallo G

Nerve growth factor regulates axial rotation during early stages of chick embryo development.
Proceedings of the National Academy of Sciences of the United States of America109(6)2009-14(2012 Feb)
Manca A,Capsoni S,Di Luzio A,Vignone D,Malerba F,Paoletti F,Brandi R,Arisi I,Cattaneo A,Levi-Montalcini R

Somatic tetraploidy in specific chick retinal ganglion cells induced by nerve growth factor.
Proceedings of the National Academy of Sciences of the United States of America107(1)109-14(2010 Jan)
Morillo SM,Escoll P,de la Hera A,Frade JM

[The expression level of NGF, BDNF and NT-3 in spinal cord of growing embryonic chicken].
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition40(4)676-8(2009 Jul)
Dai P,Xiyang YB

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NGF cDNA ORF clone, Gallus gallus(chicken)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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