ANK1 gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_015297389.1	XP_015152875.1	ankyrin-1 isoform X1
XM_015297398.1	XP_015152884.1	ankyrin-1 isoform X9
XM_015297391.2	XP_015152877.1	ankyrin-1 isoform X3
XM_015297394.2	XP_015152880.1	ankyrin-1 isoform X5
XM_015297393.1	XP_015152879.1	ankyrin-1 isoform X4
XM_015297397.1	XP_015152883.1	ankyrin-1 isoform X8
XM_015297402.1	XP_015152888.1	ankyrin-1 isoform X13
XM_015297389.2	XP_015152875.1	ankyrin-1 isoform X1
XM_015297397.2	XP_015152883.1	ankyrin-1 isoform X9
XM_015297399.2	XP_015152885.1	ankyrin-1 isoform X10
XM_015297402.2	XP_015152888.1	ankyrin-1 isoform X12
XM_015297390.1	XP_015152876.1	ankyrin-1 isoform X2
XM_015297394.1	XP_015152880.1	ankyrin-1 isoform X5
XM_015297396.1	XP_015152882.1	ankyrin-1 isoform X7
XM_015297393.2	XP_015152879.1	ankyrin-1 isoform X4
XM_015297395.2	XP_015152881.1	ankyrin-1 isoform X6
XM_015297396.2	XP_015152882.1	ankyrin-1 isoform X7
XM_015297391.1	XP_015152877.1	ankyrin-1 isoform X3
XM_015297395.1	XP_015152881.1	ankyrin-1 isoform X6
XM_015297399.1	XP_015152885.1	ankyrin-1 isoform X10
XM_015297390.2	XP_015152876.1	ankyrin-1 isoform X2
XM_025142840.1	XP_024998608.1	ankyrin-1 isoform X8
XM_015297401.2	XP_015152887.1	ankyrin-1 isoform X11
XM_015297400.1	XP_015152886.1	ankyrin-1 isoform X11
XM_015297401.1	XP_015152887.1	ankyrin-1 isoform X12

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa45396	XM_015297401.2 Latest version!	Gallus gallus ankyrin 1 (ANK1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
OGa45397	XM_015297402.2 Latest version!	Gallus gallus ankyrin 1 (ANK1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
OGa147278	XM_015297389.2 Latest version!	Gallus gallus ankyrin 1 (ANK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa147279	XM_015297390.2 Latest version!	Gallus gallus ankyrin 1 (ANK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa147280	XM_015297391.2 Latest version!	Gallus gallus ankyrin 1 (ANK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa147281	XM_015297393.2 Latest version!	Gallus gallus ankyrin 1 (ANK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa147282	XM_015297394.2 Latest version!	Gallus gallus ankyrin 1 (ANK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa147283	XM_015297395.2 Latest version!	Gallus gallus ankyrin 1 (ANK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa147284	XM_015297396.2 Latest version!	Gallus gallus ankyrin 1 (ANK1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa147285	XM_025142840.1 Latest version!	Gallus gallus ankyrin 1 (ANK1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa147286	XM_015297397.2 Latest version!	Gallus gallus ankyrin 1 (ANK1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa147287	XM_015297399.2 Latest version!	Gallus gallus ankyrin 1 (ANK1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa45385	XM_015297389.1 Latest version!	Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa45386	XM_015297390.1 Latest version!	Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa45387	XM_015297391.1 Latest version!	Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa45388	XM_015297393.1 Latest version!	Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa45389	XM_015297394.1 Latest version!	Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa45390	XM_015297395.1 Latest version!	Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa45391	XM_015297396.1 Latest version!	Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa45392	XM_015297397.1 Latest version!	Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa45393	XM_015297398.1 Latest version!	Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa45394	XM_015297399.1 Latest version!	Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa45395	XM_015297400.1 Latest version!	Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
View Old Accession Versions >>
OGa45397	XM_015297402.1	Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
OGa45396	XM_015297401.1	Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
Hide Old Accession Versions >>

ANK1 ( XM_015297401.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152887.1 Gallus gallus ankyrin 1 (ANK1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OGa45396
Clone ID Related Accession (Same CDS sequence)	XM_015297401.2 , XM_015297401.1
Accession Version	XM_015297401.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ankyrin-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006109.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015297401.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTT TCCTGGTCCA GCTGCTGATT GTCGTGGTGC TGCTGGCCTT CTTCCTGGTC 
AGCTGCCAGA ATGTCCTGTA CATCGTCAGG GGCTCTGTGC GCTTCCTGCT CAATCATGCG 
CACCGTGAGC TGGACAAGGA GCTCGGGGAG AGCCAGGGGC TGGCGGACGA CGAGGAGTCT 
CTCTCTACCA GAGTCGTCCG CCGGCGTGTC CTTGTGAAGG GCAACGAAGT CCTCCATCTC 
CCTGGGGAGC ATGTGACTGA GGAGCAGTTC ACAGATGAAC AAGGCAATAT AATCACCAAG 
AAGGTCATCC GGAAAGTGGT GCATCAGTTG GACCTCGACA CGGATGACAG GAAGGAGCAT 
GAGGACTTGA TTCTGGAGGG CTCCCTGCAG GAGCCCCAAG ACCTGGAGGC AGAGGCTGAT 
CACTTCATGT ACTCCATCCT ACAGCGGGAC GGTCTGGGGG CCAAGGAGGA GGTGCAAGTG 
CGTGTCCCAA AACTGGAAGT CTCCGGGAGC AGGATGGGGG CTCAGATAGT GAAACGAGCC 
AGTCTGAAAA GGGGGAAGCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152887.1
CDS	706..1269
Translation	MWSFLVQLLIVVVLLAFFLVSCQNVLYIVRGSVRFLLNHAHRELDKELGESQGLADDEESLSTRVVRRRVLVKGNEVLHLPGEHVTEEQFTDEQGNIITKKVIRKVVHQLDLDTDDRKEHEDLILEGSLQEPQDLEAEADHFMYSILQRDGLGAKEEVQVRVPKLEVSGSRMGAQIVKRASLKRGKH

Target ORF information:

RefSeq Version	XM_015297401.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ankyrin 1 (ANK1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297401.2

ORF Insert Sequence:

ATGTGGTCTT TCCTGGTCCA GCTGCTGATT GTCGTGGTGC TGCTGGCCTT CTTCCTGGTC 
AGCTGCCAGA ATGTCCTGTA CATCGTCAGG GGCTCTGTGC GCTTCCTGCT CAATCATGCG 
CACCGTGAGC TGGACAAGGA GCTCGGGGAG AGCCAGGGGC TGGCGGACGA CGAGGAGTCT 
CTCTCTACCA GAGTCGTCCG CCGGCGTGTC CTTGTGAAGG GCAACGAAGT CCTCCATCTC 
CCTGGGGAGC ATGTGACTGA GGAGCAGTTC ACAGATGAAC AAGGCAATAT AATCACCAAG 
AAGGTCATCC GGAAAGTGGT GCATCAGTTG GACCTCGACA CGGATGACAG GAAGGAGCAT 
GAGGACTTGA TTCTGGAGGG CTCCCTGCAG GAGCCCCAAG ACCTGGAGGC AGAGGCTGAT 
CACTTCATGT ACTCCATCCT ACAGCGGGAC GGTCTGGGGG CCAAGGAGGA GGTGCAAGTG 
CGTGTCCCAA AACTGGAAGT CTCCGGGAGC AGGATGGGGG CTCAGATAGT GAAACGAGCC 
AGTCTGAAAA GGGGGAAGCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK1 ( XM_015297401.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152887.1 Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OGa45396
Clone ID Related Accession (Same CDS sequence)	XM_015297401.2 , XM_015297401.1
Accession Version	XM_015297401.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ankyrin-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455999.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTT TCCTGGTCCA GCTGCTGATT GTCGTGGTGC TGCTGGCCTT CTTCCTGGTC 
AGCTGCCAGA ATGTCCTGTA CATCGTCAGG GGCTCTGTGC GCTTCCTGCT CAATCATGCG 
CACCGTGAGC TGGACAAGGA GCTCGGGGAG AGCCAGGGGC TGGCGGACGA CGAGGAGTCT 
CTCTCTACCA GAGTCGTCCG CCGGCGTGTC CTTGTGAAGG GCAACGAAGT CCTCCATCTC 
CCTGGGGAGC ATGTGACTGA GGAGCAGTTC ACAGATGAAC AAGGCAATAT AATCACCAAG 
AAGGTCATCC GGAAAGTGGT GCATCAGTTG GACCTCGACA CGGATGACAG GAAGGAGCAT 
GAGGACTTGA TTCTGGAGGG CTCCCTGCAG GAGCCCCAAG ACCTGGAGGC AGAGGCTGAT 
CACTTCATGT ACTCCATCCT ACAGCGGGAC GGTCTGGGGG CCAAGGAGGA GGTGCAAGTG 
CGTGTCCCAA AACTGGAAGT CTCCGGGAGC AGGATGGGGG CTCAGATAGT GAAACGAGCC 
AGTCTGAAAA GGGGGAAGCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152887.1
CDS	711..1274
Translation	MWSFLVQLLIVVVLLAFFLVSCQNVLYIVRGSVRFLLNHAHRELDKELGESQGLADDEESLSTRVVRRRVLVKGNEVLHLPGEHVTEEQFTDEQGNIITKKVIRKVVHQLDLDTDDRKEHEDLILEGSLQEPQDLEAEADHFMYSILQRDGLGAKEEVQVRVPKLEVSGSRMGAQIVKRASLKRGKH

Target ORF information:

RefSeq Version	XM_015297401.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297401.1

ORF Insert Sequence:

ATGTGGTCTT TCCTGGTCCA GCTGCTGATT GTCGTGGTGC TGCTGGCCTT CTTCCTGGTC 
AGCTGCCAGA ATGTCCTGTA CATCGTCAGG GGCTCTGTGC GCTTCCTGCT CAATCATGCG 
CACCGTGAGC TGGACAAGGA GCTCGGGGAG AGCCAGGGGC TGGCGGACGA CGAGGAGTCT 
CTCTCTACCA GAGTCGTCCG CCGGCGTGTC CTTGTGAAGG GCAACGAAGT CCTCCATCTC 
CCTGGGGAGC ATGTGACTGA GGAGCAGTTC ACAGATGAAC AAGGCAATAT AATCACCAAG 
AAGGTCATCC GGAAAGTGGT GCATCAGTTG GACCTCGACA CGGATGACAG GAAGGAGCAT 
GAGGACTTGA TTCTGGAGGG CTCCCTGCAG GAGCCCCAAG ACCTGGAGGC AGAGGCTGAT 
CACTTCATGT ACTCCATCCT ACAGCGGGAC GGTCTGGGGG CCAAGGAGGA GGTGCAAGTG 
CGTGTCCCAA AACTGGAAGT CTCCGGGAGC AGGATGGGGG CTCAGATAGT GAAACGAGCC 
AGTCTGAAAA GGGGGAAGCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK1 ( XM_015297402.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152888.1 Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OGa45397
Clone ID Related Accession (Same CDS sequence)	XM_015297402.1 , XM_015297402.2
Accession Version	XM_015297402.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ankyrin-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455999.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTT TCCTGGTCCA GCTGCTGATT GTCGTGGTGC TGCTGGCCTT CTTCCTGGTC 
AGCTGCCAGA ATGTCCTGTA CATCGTCAGG GGCTCTGTGC GCTTCCTGCT CAATCATGCG 
CACCGTGAGC TGGACAAGGA GCTCGGGGAG AGCCAGGGGC TGGCGGACGA CGAGGAGTCT 
CTCTCTACCA GAGTCGTCCG CCGGCGTGTC CTTGTGAAGG GCAACGAAGT CCTCCATCTC 
CCTGGGGAGC ATGTGACTGA GGAGCAGTTC ACAGATGAAC AAGGCAATAT AATCACCAAG 
AAGGTCATCC GGAAAGTGGT GCATCAGTTG GACCTCGACA CGGATGACAG GAAGGAGCAT 
GAGGACTTGA TTCTGGAGGG CTCCCTGCAG GAGCCCCAAG ACCTGGAGGC AGAGGCTGAT 
CACTTCATGT ACTCCATCCT ACAGCGGGAC GGTCTGGGGG CCAAGGATCT CACCTCAACA 
CCAAACCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152888.1
CDS	706..1197
Translation	MWSFLVQLLIVVVLLAFFLVSCQNVLYIVRGSVRFLLNHAHRELDKELGESQGLADDEESLSTRVVRRRVLVKGNEVLHLPGEHVTEEQFTDEQGNIITKKVIRKVVHQLDLDTDDRKEHEDLILEGSLQEPQDLEAEADHFMYSILQRDGLGAKDLTSTPNH

Target ORF information:

RefSeq Version	XM_015297402.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297402.1

ORF Insert Sequence:

ATGTGGTCTT TCCTGGTCCA GCTGCTGATT GTCGTGGTGC TGCTGGCCTT CTTCCTGGTC 
AGCTGCCAGA ATGTCCTGTA CATCGTCAGG GGCTCTGTGC GCTTCCTGCT CAATCATGCG 
CACCGTGAGC TGGACAAGGA GCTCGGGGAG AGCCAGGGGC TGGCGGACGA CGAGGAGTCT 
CTCTCTACCA GAGTCGTCCG CCGGCGTGTC CTTGTGAAGG GCAACGAAGT CCTCCATCTC 
CCTGGGGAGC ATGTGACTGA GGAGCAGTTC ACAGATGAAC AAGGCAATAT AATCACCAAG 
AAGGTCATCC GGAAAGTGGT GCATCAGTTG GACCTCGACA CGGATGACAG GAAGGAGCAT 
GAGGACTTGA TTCTGGAGGG CTCCCTGCAG GAGCCCCAAG ACCTGGAGGC AGAGGCTGAT 
CACTTCATGT ACTCCATCCT ACAGCGGGAC GGTCTGGGGG CCAAGGATCT CACCTCAACA 
CCAAACCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK1 ( XM_015297402.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152888.1 Gallus gallus ankyrin 1 (ANK1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OGa45397
Clone ID Related Accession (Same CDS sequence)	XM_015297402.1 , XM_015297402.2
Accession Version	XM_015297402.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ankyrin-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006109.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015297402.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTT TCCTGGTCCA GCTGCTGATT GTCGTGGTGC TGCTGGCCTT CTTCCTGGTC 
AGCTGCCAGA ATGTCCTGTA CATCGTCAGG GGCTCTGTGC GCTTCCTGCT CAATCATGCG 
CACCGTGAGC TGGACAAGGA GCTCGGGGAG AGCCAGGGGC TGGCGGACGA CGAGGAGTCT 
CTCTCTACCA GAGTCGTCCG CCGGCGTGTC CTTGTGAAGG GCAACGAAGT CCTCCATCTC 
CCTGGGGAGC ATGTGACTGA GGAGCAGTTC ACAGATGAAC AAGGCAATAT AATCACCAAG 
AAGGTCATCC GGAAAGTGGT GCATCAGTTG GACCTCGACA CGGATGACAG GAAGGAGCAT 
GAGGACTTGA TTCTGGAGGG CTCCCTGCAG GAGCCCCAAG ACCTGGAGGC AGAGGCTGAT 
CACTTCATGT ACTCCATCCT ACAGCGGGAC GGTCTGGGGG CCAAGGATCT CACCTCAACA 
CCAAACCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152888.1
CDS	700..1191
Translation	MWSFLVQLLIVVVLLAFFLVSCQNVLYIVRGSVRFLLNHAHRELDKELGESQGLADDEESLSTRVVRRRVLVKGNEVLHLPGEHVTEEQFTDEQGNIITKKVIRKVVHQLDLDTDDRKEHEDLILEGSLQEPQDLEAEADHFMYSILQRDGLGAKDLTSTPNH

Target ORF information:

RefSeq Version	XM_015297402.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ankyrin 1 (ANK1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297402.2

ORF Insert Sequence:

ATGTGGTCTT TCCTGGTCCA GCTGCTGATT GTCGTGGTGC TGCTGGCCTT CTTCCTGGTC 
AGCTGCCAGA ATGTCCTGTA CATCGTCAGG GGCTCTGTGC GCTTCCTGCT CAATCATGCG 
CACCGTGAGC TGGACAAGGA GCTCGGGGAG AGCCAGGGGC TGGCGGACGA CGAGGAGTCT 
CTCTCTACCA GAGTCGTCCG CCGGCGTGTC CTTGTGAAGG GCAACGAAGT CCTCCATCTC 
CCTGGGGAGC ATGTGACTGA GGAGCAGTTC ACAGATGAAC AAGGCAATAT AATCACCAAG 
AAGGTCATCC GGAAAGTGGT GCATCAGTTG GACCTCGACA CGGATGACAG GAAGGAGCAT 
GAGGACTTGA TTCTGGAGGG CTCCCTGCAG GAGCCCCAAG ACCTGGAGGC AGAGGCTGAT 
CACTTCATGT ACTCCATCCT ACAGCGGGAC GGTCTGGGGG CCAAGGATCT CACCTCAACA 
CCAAACCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK1 ( XM_015297389.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152875.1 Gallus gallus ankyrin 1 (ANK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa147278
Clone ID Related Accession (Same CDS sequence)	XM_015297389.2
Accession Version	XM_015297389.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ankyrin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006109.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015297389.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGAGAG GGCATGCACC AACCACTGTG GACCCCTTGC CTTCTTGTTG CAAGCAAGGA 
TCTCCTGAAG GGATGCTGGA GCAGGAGTCT GCTGTGCAGG CTGATGCTGC GACCAGCTTC 
CTGAGAGCTG CAAGATCTGG GAATCTGGAC AGAGCCTTGG ATCATCTCAG GAATGGGGTA 
GATATTAACA CCTGTAACCA GAATGGGCTG AATGCCTTGC ACCTGGCCTC CAAGGAGGGC 
CACACGAAAA TGGTGGTGGA GCTTCTGCAC AAGGAGATTG TTTTGGAGAC CACTACCAAG 
AAGGGAAACA CAGCCCTGCA CATCGCTGCC CTCGCTGGAC AGCAGGACGT GGTCCGGGAA 
CTGGTGAACT ATGGGGCCAA TGTCAATGCT CAGTCGCAGA AGGGCTTCAC ACCACTCTAC 
ATGGCAGCGC AGGAAAACCA CCTGGAAGTT GTCAAATTCC TGCTGGAAAA CGGAGCCAAC 
CAGAATGTAG CCACAGAGGA TGGCTTCACA CCACTAGCTG TGGCTCTGCA GCAGGGTCAT 
GAGAATGTGG TTGCTCACCT TATCAACTAT GGGACGAAGG GTAAGGTCCG CCTGCCTGCC 
TTGCACATTG CAGCTCGCAA CGATGACACC CGTACAGCCG CCGTGCTGCT GCAGAATGAC 
CCCAATGCTG ACGTCCTCTC CAAGACTGGA TTTACACCTT TGCACATTGC AGCCCACTAT 
GAGAATCTCA GCGTGGCCCA ATTATTGCTG AATCGTGGAG CCAGCGTCAA CTTCACACCC 
CAGAATGGGA TCACTCCTTT GCACATAGCC TCTCGTCGAG GCAACATCAT CATGGTACGG 
CTGCTGCTGG ACCGTGGGGC CCAGATAGAG ACAAGGACCA AGGATGAGTT GACGCCTCTC 
CACTGTGCAG CTCGCAATGG ACATGTGCGA ATTGCAGAGA TCCTACTGGA CCATGGTGCT 
CCCATTCAAG CCAAAACAAA GAACGGTCTG TCACCGATCC ACATGGCAGC ACAGGGTGAC 
CACCTGGACT GTGTGCGCCT ACTCCTGCAG TACAGTGCTG AGATAGACGA CATCACCCTG 
GACCACCTGA CACCACTGCA TGTAGCTGCG CACTGTGGCC ACCACCGTGT GGCCAAGCTG 
CTGGTGGAGA AGGGAGCCAA GCCCAACTCC AGAGCTCTGA ATGGCTTCAC GCCTCTCCAC 
ATTGCCTGCA AGAAGAACCA CATTCGGGTG ATGGAGTTGC TTCTGAAGAC AGGAGCCTCC 
ATTGATGCTG TCACAGAGTC TGGCCTGACC CCCTTGCATG TGGCTGCCTT CATGGGGCAC 
TTGCCCATTG TCAAAACCTT GCTGCAGCGT GGAGCATCTC CTAATGTGTC CAATGTGAAA 
GTGGAGACTC CCCTGCACAT GGCAGCCAGA GCTGGGCACA TGGATGTGGC CAAGTATCTG 
ATACAGAACA AGGCCAAAAT CAACGCCAAG GCCAAGGATG ACCAGACTCC TCTGCACTGT 
GCTGCACGCA TTGGCCACAC CAGCATGGTC CAACTCCTGC TGGAGAACAA TGCCGACCCC 
AACCTGGCCA CTACAGCAGG GCACACGCCT CTGCACATCG CTGCCAGAGA GGGGCATGTA 
GACACAGCCT TGGCCCTGCT AGAGAAGGGA GCCTCTCAGA CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TCCACGTTGC AGCCAAGTAT GGGAAGGTGG ATGTGGCAGA GCTGCTGCTG 
GTGCATGATG CTCACCCCAA TGCAGCGGGG AAGAACGGCC TGACCCCACT GCACGTAGCT 
GTGCACCACA ACAACCTGGA GATCGTCAAG CTTCTGCTTC CCAAGGGGAG CTCTCCACAC 
AGCTCAGCCT GGAACGGGTA CACCCCTCTG CACATTGCCG CCAAGCAGAA CCAGATGGAG 
GTGGCCAGCA GCTTGCTGCA GTATGGTGCT TCTGCAAATG CGGAGTCTGT TCAGGGAGTC 
ACCCCTCTGC ACCTGGCTTC TCAGGAAGGG CATACAGACA TGGTAGCACT GCTTTTCTCC 
AAGCAAGCCA ATGGAAACTT AGGCAACAAG AGTGGTCTGA CTCCTCTCCA TCTCGTGGCC 
CAAGAGGGAC ACGTACCAGT GGCTGATGTT CTGGTGAAAC ATGGAGTCAC AGTGGATGCG 
ACAACCAGGA TGGGCTACAC CTCACTGCAC ATAGCCAGCC ACTATGGGAA CATCAAGTTG 
GTGAAGTTTC TGCTGCAGCA CCAGGCTGAT GTCAATGCTA AGACTAAGCT GGGCTACACA 
CCCCTGCACC AGGCGGCACA GCAGGGCCAC ACGGATGTGG TGACCCTGCT GCTGAAGCAT 
GGTGCCTCTC CCAACGAGAT CAGCACAAAT GGCACCACTC CCCTGGCCAT TGCGAAGCGC 
CTCGGTTACA TTTCCGTCAC AGATGTGCTC AAGATAGTCA CAGAGGAGAC CACCATCCCA 
CCATTCAGTG ACAAGTACCG CATGAGCTTC CCGGAGACTG TGGATGAGAT TCTGGATGTG 
TCGGAGGATG AAGGCACTGC TCATGTCACA GTAATGGAGG AGGAGCTGGT CCCACCGAAA 
CCCAGAACAC CTGAACCCAA GGAGCAGGAG GGCAAGAGGG AGATGCTGGA GTTTGTGACC 
GTGACAACAC TGGAGCAAAT GGAGGAATCT GCAGCTGTCC CACAGGTCCC CTGCGTGCCC 
CCTGAGACTG TGGTGACCAT AGCAGAGGAG TCTGAGCAGA TGGGACCTGT GGAGACTGAG 
GCTGAGCAAG GCGGCCTGCT GCCTGCACCC TCGGTGTCCC CACAGGAGCC CTCCAAGGAG 
TTCGATGAGG ACTCCTTGAT TCCCAGCAGC CCGGCCACCG AAACCTCGGA CAACATCAGC 
CCCGTGGCCA GCCCCGTTCA CACAGGGTTC CTGGTGAGCT TCATGGTGGA TGCGCGTGGC 
GGCTCCATGC GGGGCAGCCG GCACCACGGG CTGCGCGTGG TCATCCCTCC CCGTGCCTGT 
GCTGCACCCA CCCGCATCAC TTGCCGCCTG GTGAAGCCTC AGAAGCTGCC TGCCCCCCCA 
CCGCTGGCTG AGGAGGAGGG CCTGGCAAGC CGCATCATTG CACTGGGGCC CGCTGGCGCC 
CAGTTCCTCA GCCCCGTCAT TGTGGAGATT CCTCACTTTG CCTCCTATGG ACGTGGGGAC 
CGTGAGCTGG TGGTGTTGCG CAGTGAGAAT GGCTCTGTCT GGAAGGAGCA CCGCAACCGC 
TACGAGGAGA GCTACATGGA CCAACTGCTC AGTGGCATGG ATGAGGAGCT GGAGAGCCAA 
GAGGAGCTGG AGAAGAAGAG GGTCTGCCGC ATCATCACCA CTGACTTTCC TCTCTACTTC 
GTGGTCATGT CCCGGATTTG CCAGGACTGT GACATGATTG GCCCCGAGGG AGGGTGCTTG 
AAAAGCACAC TGGTGCCCAT GGTGCAGGCC ACCTTCCCAG ACACCGCTGT CACCAAGAAA 
GTGAGGCTGG CTCTGCAGGC GCAGCCCGTG CCTGATGAGC TGGTGACCAA GCTGCTGGGG 
AACCAGGCAA CCTTCAGCCC CATCGTCACA GTGGAGCCAC GTCGGAGGAA GTTCCACCGC 
CCCATTGGCC TCCGCATCCC TCTGCCCCCA TCCTGGAAGG ACAATCCCCG GGACAGCGGC 
GAGGGTGACA CCACCAGCCT GCGCCTGCTC TGTTCTGTGA TAGGAGGGAC AGCACAAGCT 
CAGTGGGAAG ACATCACAGG CACCACAAAG CTGGTCTATG AAAAAGAGTG TGCTAACTTT 
ACCACCAATG TGTCTGCCAG GTTCTGGCTG GCTGACTGCC CGCGCACTGC TGAGGCTGTG 
CACTTTGCCA CCGTGCTGTA CAAGGAACTG ACGGCTGTGC CCTACATGGC CAAATTTGTG 
GTGTTTGCTA AGATGAACGA TGGACGGGAA GGCCGGCTGC GCTGCTACTG CATGACTGAT 
GACAAGGTGG ACAAGACTTT GGAGCATCAT GAGAACTTCA CTGAGGTGGC CCGCAGCAGG 
GACATTGAGG TGGTGGAAGG AATGCCTCTG CATGTCGAGC TCTCAGGGAA CCTGGTGCCC 
GTCAAGAAGG CCACGCAGTC ACGCACCTTC TTCTTCCAGT CCTTCCGGGA AAGCCGCCTC 
GCCATCCCCA TCAAGGTCCG GGACAGCAGC CGGGAAGCCA GTGGCTCCCT GTCCTTCCTG 
CGCAAGGCCA TGAAGTATGA GGACTTGCAG CATGTGCTCT GCCACCTCAA CATCAGCATT 
CCACCCTGCA CCAAGGGAAG CAGCACTGAG GAGCGAAGGA GGACACTGAC ACCTTTGTCT 
CTGCGCTCTC TGAGCAGCAC CGACAGGGCA GACCAGAAGA TGATCGACAT TGCAGAGCAG 
CTTGGCCTCA GCTGGGCAGA GCTGGCACGT GAGCTGCAGT TTGGGGTGGA TGACATCAAC 
AGGATTCGTG TGGAGAACCC TAACTCCTTG CTGGAGCAGA GCATAGCCTT GCTTAACCTC 
TGGGTCAGCC GCGAGGGCAA GGGTGTCAAA ATTGAGAACC TGTACACAGC GCTGAGGAAC 
ATTGACCGCA GCGAGATCAT CAGCACGTTG GAGGGCTCCG GCCGGCAGAG CCGCAGCATG 
AAGGGCAGCT GGCGTTACAT GGACAGGGAC TATTCACTCT CGCCCTCCCA GATGAATGGT 
TACGCTTCGC TGCAGGACGA GCTGCTCTCC CCTGCCTCCC TGCATTATAT GCTGCCATCC 
CCTCTGCGTG CTGACCAGTA CTGGAACGAG GTGGCCATCA TGGACGCCAT CCCCATGGCC 
GCCACTGAGC AGGACGCCCT GATGGAGATG TCTGACATGC AGGTGTGGTC CTCAGGGCTC 
ACCCCTTCAC TGGTGACGGC TGAGGACTCC TCTCTGGAGT GCAGCAAGGC TGAGGACTCG 
GATGCTACAA GCGAAGGTCG TTTCCCAGGG CACCTTCTGA CAGATGCACA TGTCCCAGAC 
CACATGGGCT CTATGGACTT AGTTGAGGAT GACACAGTGG ACTCAGACGC CATGAACGGG 
CTGATTGAAC TGCTAGAGCA GGAGGAGGGG CAGAGGCCAG AGGGGAAGAT GCCAGCCAGC 
GACCACCAGC TAGGGACTGG AGAGCAGAAC CCAGAGAGTG AAGTCTCTTT TGTTTCAGTT 
CAACAGAAGG TACAAGCCAC GATCGTGCCA TCGCCAACCG TGAGCCACAC AGTGGAGAGG 
AGTGCAGACA GGCTGAGGGA CTGGAATGCA GAAGGCTCCT TTATCTCCTG CCTACAGGAC 
CTGACAGAGG GCTCCTGGCA GGAGGGGGTC ACCCGACGGC TGGGTCCAGC ACACACCGTG 
GCTGCTGGGG CGCAGGGCCA GGAGCAAGAG CAGGTCCTGG CGCCGGACGT GGAGCTGATG 
CGGGTCAGCT CCACAGAGGA CAGCGACTGG CAGCCCCAGC ACCCCGCGGG CGGCTGGCCA 
GAGGAGCCTG ACAGCCGCTG CTTTGGGCAG GGCAACGAAG TCCTCCATCT CCCTGGGGAG 
CATGTGACTG AGGAGCAGTT CACAGATGAA CAAGGCAATA TAATCACCAA GAAGGTCATC 
CGGAAAGTGG TGCATCAGTT GGACCTCGAC ACGGATGACA GGAAGGAGCA TGAGGACTTG 
ATTCTGGAGG GCTCCCTGCA GGAGCCCCAA GACCTGGAGG CAGAGGCTGA TCACTTCATG 
TACTCCATCC TACAGCGGGA CGGTCTGGGG GCCAAGGAGG AGGTGCAAGT GCGTGTCCCA 
AAACTGGAAG TCTCCGGGAG CAGGATGGGG GCTCAGATAG TGAAACGAGC CAGTCTGAAA 
AGGGGGAAGC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152875.1
CDS	828..6782
Translation	MAQAAKQLKKIKDIEAQALQEQKEKEESNRKRRNRSRDRKKKRGHAPTTVDPLPSCCKQGSPEGMLEQESAVQADAATSFLRAARSGNLDRALDHLRNGVDINTCNQNGLNALHLASKEGHTKMVVELLHKEIVLETTTKKGNTALHIAALAGQQDVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLSVAQLLLNRGASVNFTPQNGITPLHIASRRGNIIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRIAEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYSAEIDDITLDHLTPLHVAAHCGHHRVAKLLVEKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVAAFMGHLPIVKTLLQRGASPNVSNVKVETPLHMAARAGHMDVAKYLIQNKAKINAKAKDDQTPLHCAARIGHTSMVQLLLENNADPNLATTAGHTPLHIAAREGHVDTALALLEKGASQTCMTKKGFTPLHVAAKYGKVDVAELLLVHDAHPNAAGKNGLTPLHVAVHHNNLEIVKLLLPKGSSPHSSAWNGYTPLHIAAKQNQMEVASSLLQYGASANAESVQGVTPLHLASQEGHTDMVALLFSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLVKHGVTVDATTRMGYTSLHIASHYGNIKLVKFLLQHQADVNAKTKLGYTPLHQAAQQGHTDVVTLLLKHGASPNEISTNGTTPLAIAKRLGYISVTDVLKIVTEETTIPPFSDKYRMSFPETVDEILDVSEDEGTAHVTVMEEELVPPKPRTPEPKEQEGKREMLEFVTVTTLEQMEESAAVPQVPCVPPETVVTIAEESEQMGPVETEAEQGGLLPAPSVSPQEPSKEFDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHHGLRVVIPPRACAAPTRITCRLVKPQKLPAPPPLAEEEGLASRIIALGPAGAQFLSPVIVEIPHFASYGRGDRELVVLRSENGSVWKEHRNRYEESYMDQLLSGMDEELESQEELEKKRVCRIITTDFPLYFVVMSRICQDCDMIGPEGGCLKSTLVPMVQATFPDTAVTKKVRLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWKDNPRDSGEGDTTSLRLLCSVIGGTAQAQWEDITGTTKLVYEKECANFTTNVSARFWLADCPRTAEAVHFATVLYKELTAVPYMAKFVVFAKMNDGREGRLRCYCMTDDKVDKTLEHHENFTEVARSRDIEVVEGMPLHVELSGNLVPVKKATQSRTFFFQSFRESRLAIPIKVRDSSREASGSLSFLRKAMKYEDLQHVLCHLNISIPPCTKGSSTEERRRTLTPLSLRSLSSTDRADQKMIDIAEQLGLSWAELARELQFGVDDINRIRVENPNSLLEQSIALLNLWVSREGKGVKIENLYTALRNIDRSEIISTLEGSGRQSRSMKGSWRYMDRDYSLSPSQMNGYASLQDELLSPASLHYMLPSPLRADQYWNEVAIMDAIPMAATEQDALMEMSDMQVWSSGLTPSLVTAEDSSLECSKAEDSDATSEGRFPGHLLTDAHVPDHMGSMDLVEDDTVDSDAMNGLIELLEQEEGQRPEGKMPASDHQLGTGEQNPESEVSFVSVQQKVQATIVPSPTVSHTVERSADRLRDWNAEGSFISCLQDLTEGSWQEGVTRRLGPAHTVAAGAQGQEQEQVLAPDVELMRVSSTEDSDWQPQHPAGGWPEEPDSRCFGQGNEVLHLPGEHVTEEQFTDEQGNIITKKVIRKVVHQLDLDTDDRKEHEDLILEGSLQEPQDLEAEADHFMYSILQRDGLGAKEEVQVRVPKLEVSGSRMGAQIVKRASLKRGKH

Target ORF information:

RefSeq Version	XM_015297389.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ankyrin 1 (ANK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297389.2

ORF Insert Sequence:

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGAGAG GGCATGCACC AACCACTGTG GACCCCTTGC CTTCTTGTTG CAAGCAAGGA 
TCTCCTGAAG GGATGCTGGA GCAGGAGTCT GCTGTGCAGG CTGATGCTGC GACCAGCTTC 
CTGAGAGCTG CAAGATCTGG GAATCTGGAC AGAGCCTTGG ATCATCTCAG GAATGGGGTA 
GATATTAACA CCTGTAACCA GAATGGGCTG AATGCCTTGC ACCTGGCCTC CAAGGAGGGC 
CACACGAAAA TGGTGGTGGA GCTTCTGCAC AAGGAGATTG TTTTGGAGAC CACTACCAAG 
AAGGGAAACA CAGCCCTGCA CATCGCTGCC CTCGCTGGAC AGCAGGACGT GGTCCGGGAA 
CTGGTGAACT ATGGGGCCAA TGTCAATGCT CAGTCGCAGA AGGGCTTCAC ACCACTCTAC 
ATGGCAGCGC AGGAAAACCA CCTGGAAGTT GTCAAATTCC TGCTGGAAAA CGGAGCCAAC 
CAGAATGTAG CCACAGAGGA TGGCTTCACA CCACTAGCTG TGGCTCTGCA GCAGGGTCAT 
GAGAATGTGG TTGCTCACCT TATCAACTAT GGGACGAAGG GTAAGGTCCG CCTGCCTGCC 
TTGCACATTG CAGCTCGCAA CGATGACACC CGTACAGCCG CCGTGCTGCT GCAGAATGAC 
CCCAATGCTG ACGTCCTCTC CAAGACTGGA TTTACACCTT TGCACATTGC AGCCCACTAT 
GAGAATCTCA GCGTGGCCCA ATTATTGCTG AATCGTGGAG CCAGCGTCAA CTTCACACCC 
CAGAATGGGA TCACTCCTTT GCACATAGCC TCTCGTCGAG GCAACATCAT CATGGTACGG 
CTGCTGCTGG ACCGTGGGGC CCAGATAGAG ACAAGGACCA AGGATGAGTT GACGCCTCTC 
CACTGTGCAG CTCGCAATGG ACATGTGCGA ATTGCAGAGA TCCTACTGGA CCATGGTGCT 
CCCATTCAAG CCAAAACAAA GAACGGTCTG TCACCGATCC ACATGGCAGC ACAGGGTGAC 
CACCTGGACT GTGTGCGCCT ACTCCTGCAG TACAGTGCTG AGATAGACGA CATCACCCTG 
GACCACCTGA CACCACTGCA TGTAGCTGCG CACTGTGGCC ACCACCGTGT GGCCAAGCTG 
CTGGTGGAGA AGGGAGCCAA GCCCAACTCC AGAGCTCTGA ATGGCTTCAC GCCTCTCCAC 
ATTGCCTGCA AGAAGAACCA CATTCGGGTG ATGGAGTTGC TTCTGAAGAC AGGAGCCTCC 
ATTGATGCTG TCACAGAGTC TGGCCTGACC CCCTTGCATG TGGCTGCCTT CATGGGGCAC 
TTGCCCATTG TCAAAACCTT GCTGCAGCGT GGAGCATCTC CTAATGTGTC CAATGTGAAA 
GTGGAGACTC CCCTGCACAT GGCAGCCAGA GCTGGGCACA TGGATGTGGC CAAGTATCTG 
ATACAGAACA AGGCCAAAAT CAACGCCAAG GCCAAGGATG ACCAGACTCC TCTGCACTGT 
GCTGCACGCA TTGGCCACAC CAGCATGGTC CAACTCCTGC TGGAGAACAA TGCCGACCCC 
AACCTGGCCA CTACAGCAGG GCACACGCCT CTGCACATCG CTGCCAGAGA GGGGCATGTA 
GACACAGCCT TGGCCCTGCT AGAGAAGGGA GCCTCTCAGA CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TCCACGTTGC AGCCAAGTAT GGGAAGGTGG ATGTGGCAGA GCTGCTGCTG 
GTGCATGATG CTCACCCCAA TGCAGCGGGG AAGAACGGCC TGACCCCACT GCACGTAGCT 
GTGCACCACA ACAACCTGGA GATCGTCAAG CTTCTGCTTC CCAAGGGGAG CTCTCCACAC 
AGCTCAGCCT GGAACGGGTA CACCCCTCTG CACATTGCCG CCAAGCAGAA CCAGATGGAG 
GTGGCCAGCA GCTTGCTGCA GTATGGTGCT TCTGCAAATG CGGAGTCTGT TCAGGGAGTC 
ACCCCTCTGC ACCTGGCTTC TCAGGAAGGG CATACAGACA TGGTAGCACT GCTTTTCTCC 
AAGCAAGCCA ATGGAAACTT AGGCAACAAG AGTGGTCTGA CTCCTCTCCA TCTCGTGGCC 
CAAGAGGGAC ACGTACCAGT GGCTGATGTT CTGGTGAAAC ATGGAGTCAC AGTGGATGCG 
ACAACCAGGA TGGGCTACAC CTCACTGCAC ATAGCCAGCC ACTATGGGAA CATCAAGTTG 
GTGAAGTTTC TGCTGCAGCA CCAGGCTGAT GTCAATGCTA AGACTAAGCT GGGCTACACA 
CCCCTGCACC AGGCGGCACA GCAGGGCCAC ACGGATGTGG TGACCCTGCT GCTGAAGCAT 
GGTGCCTCTC CCAACGAGAT CAGCACAAAT GGCACCACTC CCCTGGCCAT TGCGAAGCGC 
CTCGGTTACA TTTCCGTCAC AGATGTGCTC AAGATAGTCA CAGAGGAGAC CACCATCCCA 
CCATTCAGTG ACAAGTACCG CATGAGCTTC CCGGAGACTG TGGATGAGAT TCTGGATGTG 
TCGGAGGATG AAGGCACTGC TCATGTCACA GTAATGGAGG AGGAGCTGGT CCCACCGAAA 
CCCAGAACAC CTGAACCCAA GGAGCAGGAG GGCAAGAGGG AGATGCTGGA GTTTGTGACC 
GTGACAACAC TGGAGCAAAT GGAGGAATCT GCAGCTGTCC CACAGGTCCC CTGCGTGCCC 
CCTGAGACTG TGGTGACCAT AGCAGAGGAG TCTGAGCAGA TGGGACCTGT GGAGACTGAG 
GCTGAGCAAG GCGGCCTGCT GCCTGCACCC TCGGTGTCCC CACAGGAGCC CTCCAAGGAG 
TTCGATGAGG ACTCCTTGAT TCCCAGCAGC CCGGCCACCG AAACCTCGGA CAACATCAGC 
CCCGTGGCCA GCCCCGTTCA CACAGGGTTC CTGGTGAGCT TCATGGTGGA TGCGCGTGGC 
GGCTCCATGC GGGGCAGCCG GCACCACGGG CTGCGCGTGG TCATCCCTCC CCGTGCCTGT 
GCTGCACCCA CCCGCATCAC TTGCCGCCTG GTGAAGCCTC AGAAGCTGCC TGCCCCCCCA 
CCGCTGGCTG AGGAGGAGGG CCTGGCAAGC CGCATCATTG CACTGGGGCC CGCTGGCGCC 
CAGTTCCTCA GCCCCGTCAT TGTGGAGATT CCTCACTTTG CCTCCTATGG ACGTGGGGAC 
CGTGAGCTGG TGGTGTTGCG CAGTGAGAAT GGCTCTGTCT GGAAGGAGCA CCGCAACCGC 
TACGAGGAGA GCTACATGGA CCAACTGCTC AGTGGCATGG ATGAGGAGCT GGAGAGCCAA 
GAGGAGCTGG AGAAGAAGAG GGTCTGCCGC ATCATCACCA CTGACTTTCC TCTCTACTTC 
GTGGTCATGT CCCGGATTTG CCAGGACTGT GACATGATTG GCCCCGAGGG AGGGTGCTTG 
AAAAGCACAC TGGTGCCCAT GGTGCAGGCC ACCTTCCCAG ACACCGCTGT CACCAAGAAA 
GTGAGGCTGG CTCTGCAGGC GCAGCCCGTG CCTGATGAGC TGGTGACCAA GCTGCTGGGG 
AACCAGGCAA CCTTCAGCCC CATCGTCACA GTGGAGCCAC GTCGGAGGAA GTTCCACCGC 
CCCATTGGCC TCCGCATCCC TCTGCCCCCA TCCTGGAAGG ACAATCCCCG GGACAGCGGC 
GAGGGTGACA CCACCAGCCT GCGCCTGCTC TGTTCTGTGA TAGGAGGGAC AGCACAAGCT 
CAGTGGGAAG ACATCACAGG CACCACAAAG CTGGTCTATG AAAAAGAGTG TGCTAACTTT 
ACCACCAATG TGTCTGCCAG GTTCTGGCTG GCTGACTGCC CGCGCACTGC TGAGGCTGTG 
CACTTTGCCA CCGTGCTGTA CAAGGAACTG ACGGCTGTGC CCTACATGGC CAAATTTGTG 
GTGTTTGCTA AGATGAACGA TGGACGGGAA GGCCGGCTGC GCTGCTACTG CATGACTGAT 
GACAAGGTGG ACAAGACTTT GGAGCATCAT GAGAACTTCA CTGAGGTGGC CCGCAGCAGG 
GACATTGAGG TGGTGGAAGG AATGCCTCTG CATGTCGAGC TCTCAGGGAA CCTGGTGCCC 
GTCAAGAAGG CCACGCAGTC ACGCACCTTC TTCTTCCAGT CCTTCCGGGA AAGCCGCCTC 
GCCATCCCCA TCAAGGTCCG GGACAGCAGC CGGGAAGCCA GTGGCTCCCT GTCCTTCCTG 
CGCAAGGCCA TGAAGTATGA GGACTTGCAG CATGTGCTCT GCCACCTCAA CATCAGCATT 
CCACCCTGCA CCAAGGGAAG CAGCACTGAG GAGCGAAGGA GGACACTGAC ACCTTTGTCT 
CTGCGCTCTC TGAGCAGCAC CGACAGGGCA GACCAGAAGA TGATCGACAT TGCAGAGCAG 
CTTGGCCTCA GCTGGGCAGA GCTGGCACGT GAGCTGCAGT TTGGGGTGGA TGACATCAAC 
AGGATTCGTG TGGAGAACCC TAACTCCTTG CTGGAGCAGA GCATAGCCTT GCTTAACCTC 
TGGGTCAGCC GCGAGGGCAA GGGTGTCAAA ATTGAGAACC TGTACACAGC GCTGAGGAAC 
ATTGACCGCA GCGAGATCAT CAGCACGTTG GAGGGCTCCG GCCGGCAGAG CCGCAGCATG 
AAGGGCAGCT GGCGTTACAT GGACAGGGAC TATTCACTCT CGCCCTCCCA GATGAATGGT 
TACGCTTCGC TGCAGGACGA GCTGCTCTCC CCTGCCTCCC TGCATTATAT GCTGCCATCC 
CCTCTGCGTG CTGACCAGTA CTGGAACGAG GTGGCCATCA TGGACGCCAT CCCCATGGCC 
GCCACTGAGC AGGACGCCCT GATGGAGATG TCTGACATGC AGGTGTGGTC CTCAGGGCTC 
ACCCCTTCAC TGGTGACGGC TGAGGACTCC TCTCTGGAGT GCAGCAAGGC TGAGGACTCG 
GATGCTACAA GCGAAGGTCG TTTCCCAGGG CACCTTCTGA CAGATGCACA TGTCCCAGAC 
CACATGGGCT CTATGGACTT AGTTGAGGAT GACACAGTGG ACTCAGACGC CATGAACGGG 
CTGATTGAAC TGCTAGAGCA GGAGGAGGGG CAGAGGCCAG AGGGGAAGAT GCCAGCCAGC 
GACCACCAGC TAGGGACTGG AGAGCAGAAC CCAGAGAGTG AAGTCTCTTT TGTTTCAGTT 
CAACAGAAGG TACAAGCCAC GATCGTGCCA TCGCCAACCG TGAGCCACAC AGTGGAGAGG 
AGTGCAGACA GGCTGAGGGA CTGGAATGCA GAAGGCTCCT TTATCTCCTG CCTACAGGAC 
CTGACAGAGG GCTCCTGGCA GGAGGGGGTC ACCCGACGGC TGGGTCCAGC ACACACCGTG 
GCTGCTGGGG CGCAGGGCCA GGAGCAAGAG CAGGTCCTGG CGCCGGACGT GGAGCTGATG 
CGGGTCAGCT CCACAGAGGA CAGCGACTGG CAGCCCCAGC ACCCCGCGGG CGGCTGGCCA 
GAGGAGCCTG ACAGCCGCTG CTTTGGGCAG GGCAACGAAG TCCTCCATCT CCCTGGGGAG 
CATGTGACTG AGGAGCAGTT CACAGATGAA CAAGGCAATA TAATCACCAA GAAGGTCATC 
CGGAAAGTGG TGCATCAGTT GGACCTCGAC ACGGATGACA GGAAGGAGCA TGAGGACTTG 
ATTCTGGAGG GCTCCCTGCA GGAGCCCCAA GACCTGGAGG CAGAGGCTGA TCACTTCATG 
TACTCCATCC TACAGCGGGA CGGTCTGGGG GCCAAGGAGG AGGTGCAAGT GCGTGTCCCA 
AAACTGGAAG TCTCCGGGAG CAGGATGGGG GCTCAGATAG TGAAACGAGC CAGTCTGAAA 
AGGGGGAAGC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK1 ( XM_015297390.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152876.1 Gallus gallus ankyrin 1 (ANK1), transcript variant X2, mRNA.

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CloneID	OGa147279
Clone ID Related Accession (Same CDS sequence)	XM_015297390.2
Accession Version	XM_015297390.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ankyrin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006109.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015297390.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGAGAG GGCATGCACC AACCACTGTG GACCCCTTGC CTTCTTGTTG CAAGCAAGGA 
TCTCCTGAAG GGATGCTGGA GCAGGAGTCT GCTGTGCAGG CTGATGCTGC GACCAGCTTC 
CTGAGAGCTG CAAGATCTGG GAATCTGGAC AGAGCCTTGG ATCATCTCAG GAATGGGGTA 
GATATTAACA CCTGTAACCA GAATGGGCTG AATGCCTTGC ACCTGGCCTC CAAGGAGGGC 
CACACGAAAA TGGTGGTGGA GCTTCTGCAC AAGGAGATTG TTTTGGAGAC CACTACCAAG 
AAGGGAAACA CAGCCCTGCA CATCGCTGCC CTCGCTGGAC AGCAGGACGT GGTCCGGGAA 
CTGGTGAACT ATGGGGCCAA TGTCAATGCT CAGTCGCAGA AGGGCTTCAC ACCACTCTAC 
ATGGCAGCGC AGGAAAACCA CCTGGAAGTT GTCAAATTCC TGCTGGAAAA CGGAGCCAAC 
CAGAATGTAG CCACAGAGGA TGGCTTCACA CCACTAGCTG TGGCTCTGCA GCAGGGTCAT 
GAGAATGTGG TTGCTCACCT TATCAACTAT GGGACGAAGG GTAAGGTCCG CCTGCCTGCC 
TTGCACATTG CAGCTCGCAA CGATGACACC CGTACAGCCG CCGTGCTGCT GCAGAATGAC 
CCCAATGCTG ACGTCCTCTC CAAGACTGGA TTTACACCTT TGCACATTGC AGCCCACTAT 
GAGAATCTCA GCGTGGCCCA ATTATTGCTG AATCGTGGAG CCAGCGTCAA CTTCACACCC 
CAGAATGGGA TCACTCCTTT GCACATAGCC TCTCGTCGAG GCAACATCAT CATGGTACGG 
CTGCTGCTGG ACCGTGGGGC CCAGATAGAG ACAAGGACCA AGGATGAGTT GACGCCTCTC 
CACTGTGCAG CTCGCAATGG ACATGTGCGA ATTGCAGAGA TCCTACTGGA CCATGGTGCT 
CCCATTCAAG CCAAAACAAA GAACGGTCTG TCACCGATCC ACATGGCAGC ACAGGGTGAC 
CACCTGGACT GTGTGCGCCT ACTCCTGCAG TACAGTGCTG AGATAGACGA CATCACCCTG 
GACCACCTGA CACCACTGCA TGTAGCTGCG CACTGTGGCC ACCACCGTGT GGCCAAGCTG 
CTGGTGGAGA AGGGAGCCAA GCCCAACTCC AGAGCTCTGA ATGGCTTCAC GCCTCTCCAC 
ATTGCCTGCA AGAAGAACCA CATTCGGGTG ATGGAGTTGC TTCTGAAGAC AGGAGCCTCC 
ATTGATGCTG TCACAGAGTC TGGCCTGACC CCCTTGCATG TGGCTGCCTT CATGGGGCAC 
TTGCCCATTG TCAAAACCTT GCTGCAGCGT GGAGCATCTC CTAATGTGTC CAATGTGAAA 
GTGGAGACTC CCCTGCACAT GGCAGCCAGA GCTGGGCACA TGGATGTGGC CAAGTATCTG 
ATACAGAACA AGGCCAAAAT CAACGCCAAG GCCAAGGATG ACCAGACTCC TCTGCACTGT 
GCTGCACGCA TTGGCCACAC CAGCATGGTC CAACTCCTGC TGGAGAACAA TGCCGACCCC 
AACCTGGCCA CTACAGCAGG GCACACGCCT CTGCACATCG CTGCCAGAGA GGGGCATGTA 
GACACAGCCT TGGCCCTGCT AGAGAAGGGA GCCTCTCAGA CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TCCACGTTGC AGCCAAGTAT GGGAAGGTGG ATGTGGCAGA GCTGCTGCTG 
GTGCATGATG CTCACCCCAA TGCAGCGGGG AAGAACGGCC TGACCCCACT GCACGTAGCT 
GTGCACCACA ACAACCTGGA GATCGTCAAG CTTCTGCTTC CCAAGGGGAG CTCTCCACAC 
AGCTCAGCCT GGAACGGGTA CACCCCTCTG CACATTGCCG CCAAGCAGAA CCAGATGGAG 
GTGGCCAGCA GCTTGCTGCA GTATGGTGCT TCTGCAAATG CGGAGTCTGT TCAGGGAGTC 
ACCCCTCTGC ACCTGGCTTC TCAGGAAGGG CATACAGACA TGGTAGCACT GCTTTTCTCC 
AAGCAAGCCA ATGGAAACTT AGGCAACAAG AGTGGTCTGA CTCCTCTCCA TCTCGTGGCC 
CAAGAGGGAC ACGTACCAGT GGCTGATGTT CTGGTGAAAC ATGGAGTCAC AGTGGATGCG 
ACAACCAGGA TGGGCTACAC CTCACTGCAC ATAGCCAGCC ACTATGGGAA CATCAAGTTG 
GTGAAGTTTC TGCTGCAGCA CCAGGCTGAT GTCAATGCTA AGACTAAGCT GGGCTACACA 
CCCCTGCACC AGGCGGCACA GCAGGGCCAC ACGGATGTGG TGACCCTGCT GCTGAAGCAT 
GGTGCCTCTC CCAACGAGAT CAGCACAAAT GGCACCACTC CCCTGGCCAT TGCGAAGCGC 
CTCGGTTACA TTTCCGTCAC AGATGTGCTC AAGATAGTCA CAGAGGAGAC CACCATCCCA 
CCATTCAGTG ACAAGTACCG CATGAGCTTC CCGGAGACTG TGGATGAGAT TCTGGATGTG 
TCGGAGGATG AAGGCACTGC TCATGTCACA GTAATGGAGG AGGAGCTGGT CCCACCGAAA 
CCCAGAACAC CTGAACCCAA GGAGCAGGAG GGCAAGAGGG AGATGCTGGA GTTTGTGACC 
GTGACAACAC TGGAGCAAAT GGAGGAATCT GCAGCTGTCC CACAGGTCCC CTGCGTGCCC 
CCTGAGACTG TGGTGACCAT AGCAGAGGAG TCTGAGCAGA TGGGACCTGT GGAGACTGAG 
GCTGAGCAAG GCGGCCTGCT GCCTGCACCC TCGGTGTCCC CACAGGAGCC CTCCAAGGAG 
TTCGATGAGG ACTCCTTGAT TCCCAGCAGC CCGGCCACCG AAACCTCGGA CAACATCAGC 
CCCGTGGCCA GCCCCGTTCA CACAGGGTTC CTGGTGAGCT TCATGGTGGA TGCGCGTGGC 
GGCTCCATGC GGGGCAGCCG GCACCACGGG CTGCGCGTGG TCATCCCTCC CCGTGCCTGT 
GCTGCACCCA CCCGCATCAC TTGCCGCCTG GTGAAGCCTC AGAAGCTGCC TGCCCCCCCA 
CCGCTGGCTG AGGAGGAGGG CCTGGCAAGC CGCATCATTG CACTGGGGCC CGCTGGCGCC 
CAGTTCCTCA GCCCCGTCAT TGTGGAGATT CCTCACTTTG CCTCCTATGG ACGTGGGGAC 
CGTGAGCTGG TGGTGTTGCG CAGTGAGAAT GGCTCTGTCT GGAAGGAGCA CCGCAACCGC 
TACGAGGAGA GCTACATGGA CCAACTGCTC AGTGGCATGG ATGAGGAGCT GGAGAGCCAA 
GAGGAGCTGG AGAAGAAGAG GGTCTGCCGC ATCATCACCA CTGACTTTCC TCTCTACTTC 
GTGGTCATGT CCCGGATTTG CCAGGACTGT GACATGATTG GCCCCGAGGG AGGGTGCTTG 
AAAAGCACAC TGGTGCCCAT GGTGCAGGCC ACCTTCCCAG ACACCGCTGT CACCAAGAAA 
GTGAGGCTGG CTCTGCAGGC GCAGCCCGTG CCTGATGAGC TGGTGACCAA GCTGCTGGGG 
AACCAGGCAA CCTTCAGCCC CATCGTCACA GTGGAGCCAC GTCGGAGGAA GTTCCACCGC 
CCCATTGGCC TCCGCATCCC TCTGCCCCCA TCCTGGAAGG ACAATCCCCG GGACAGCGGC 
GAGGGTGACA CCACCAGCCT GCGCCTGCTC TGTTCTGTGA TAGGAGGGAC AGCACAAGCT 
CAGTGGGAAG ACATCACAGG CACCACAAAG CTGGTCTATG AAAAAGAGTG TGCTAACTTT 
ACCACCAATG TGTCTGCCAG GTTCTGGCTG GCTGACTGCC CGCGCACTGC TGAGGCTGTG 
CACTTTGCCA CCGTGCTGTA CAAGGAACTG ACGGCTGTGC CCTACATGGC CAAATTTGTG 
GTGTTTGCTA AGATGAACGA TGGACGGGAA GGCCGGCTGC GCTGCTACTG CATGACTGAT 
GACAAGGTGG ACAAGACTTT GGAGCATCAT GAGAACTTCA CTGAGGTGGC CCGCAGCAGG 
GACATTGAGG TGGTGGAAGG AATGCCTCTG CATGTCGAGC TCTCAGGGAA CCTGGTGCCC 
GTCAAGAAGG CCACGCAGTC ACGCACCTTC TTCTTCCAGT CCTTCCGGGA AAGCCGCCTC 
GCCATCCCCA TCAAGGTCCG GGACAGCAGC CGGGAAGCCA GTGGCTCCCT GTCCTTCCTG 
CGCAAGGCCA TGAAGTATGA GGACTTGCAG CATGTGCTCT GCCACCTCAA CATCAGCATT 
CCACCCTGCA CCAAGGGAAG CAGCACTGAG GAGCGAAGGA GGACACTGAC ACCTTTGTCT 
CTGCGCTCTC TGAGCAGCAC CGACAGGGCA GACCAGAAGA TGATCGACAT TGCAGAGCAG 
CTTGGCCTCA GCTGGGCAGA GCTGGCACGT GAGCTGCAGT TTGGGGTGGA TGACATCAAC 
AGGATTCGTG TGGAGAACCC TAACTCCTTG CTGGAGCAGA GCATAGCCTT GCTTAACCTC 
TGGGTCAGCC GCGAGGGCAA GGGTGTCAAA ATTGAGAACC TGTACACAGC GCTGAGGAAC 
ATTGACCGCA GCGAGATCAT CAGCACGTTG GAGGGCTCCG GCCGGCAGAG CCGCAGCATG 
AAGGGCAGCT GGCGTTACAT GGACAGGGAC TATTCACTCT CGCCCTCCCA GATGAATGGT 
TACGCTTCGC TGCAGGACGA GCTGCTCTCC CCTGCCTCCC TGCATTATAT GCTGCCATCC 
CCTCTGCGTG CTGACCAGTA CTGGAACGAG GTGGCCATCA TGGACGCCAT CCCCATGGCC 
GCCACTGAGC AGGACGCCCT GATGGAGATG TCTGACATGC AGGTGTGGTC CTCAGGGCTC 
ACCCCTTCAC TGGTGACGGC TGAGGACTCC TCTCTGGAGT GCAGCAAGGC TGAGGACTCG 
GATGCTACAA GCGAAGGTCG TTTCCCAGGG CACCTTCTGA CAGATGCACA TGTCCCAGAC 
CACATGGGCT CTATGGACTT AGTTGAGGAT GACACAGTGG ACTCAGACGC CATGAACGGG 
CTGATTGAAC TGCTAGAGCA GGAGGAGGGG CAGAGGCCAG AGGGGAAGAT GCCAGCCAGC 
GACCACCAGC TAGGGACTGG AGAGCAGAAC CCAGAGAGTG AAGTCTCTTT TGTTTCAGTT 
CAACAGAAGG TACAAGCCAC GATCGTGCCA TCGCCAACCG TGAGCCACAC AGTGGAGAGG 
AGTGCAGACA GGCTGAGGGA CTGGAATGCA GAAGGCTCCT TTATCTCCTG CCTACAGGAC 
CTGACAGAGG GCTCCTGGCA GGAGGGGGTC ACCCGACGGC TGGGTCCAGC ACACACCGTG 
GCTGCTGGGG CGCAGGGCCA GGAGCAAGAG CAGGTCCTGG CGCCGGACGT GGAGCTGATG 
CGGGTCAGCT CCACAGAGGA CAGCGACTGG CAGCCCCAGC ACCCCGCGGG CGGCTGGCCA 
GAGGAGCCTG ACAGCCGCTG CTTTGGGCAG GGCAACGAAG TCCTCCATCT CCCTGGGGAG 
CATGTGACTG AGGAGCAGTT CACAGATGAA CAAGGCAATA TAATCACCAA GAAGGTCATC 
CGGAAAGTGG TGCATCAGTT GGACCTCGAC ACGGATGACA GGAAGGAGCA TGAGGACTTG 
ATTCTGGAGG GCTCCCTGCA GGAGCCCCAA GACCTGGAGG CAGAGGCTGA TCACTTCATG 
TACTCCATCC TACAGCGGGA CGGTCTGGGG GCCAAGGATC TCACCTCAAC ACCAAACCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152876.1
CDS	824..6706
Translation	MAQAAKQLKKIKDIEAQALQEQKEKEESNRKRRNRSRDRKKKRGHAPTTVDPLPSCCKQGSPEGMLEQESAVQADAATSFLRAARSGNLDRALDHLRNGVDINTCNQNGLNALHLASKEGHTKMVVELLHKEIVLETTTKKGNTALHIAALAGQQDVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLSVAQLLLNRGASVNFTPQNGITPLHIASRRGNIIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRIAEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYSAEIDDITLDHLTPLHVAAHCGHHRVAKLLVEKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVAAFMGHLPIVKTLLQRGASPNVSNVKVETPLHMAARAGHMDVAKYLIQNKAKINAKAKDDQTPLHCAARIGHTSMVQLLLENNADPNLATTAGHTPLHIAAREGHVDTALALLEKGASQTCMTKKGFTPLHVAAKYGKVDVAELLLVHDAHPNAAGKNGLTPLHVAVHHNNLEIVKLLLPKGSSPHSSAWNGYTPLHIAAKQNQMEVASSLLQYGASANAESVQGVTPLHLASQEGHTDMVALLFSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLVKHGVTVDATTRMGYTSLHIASHYGNIKLVKFLLQHQADVNAKTKLGYTPLHQAAQQGHTDVVTLLLKHGASPNEISTNGTTPLAIAKRLGYISVTDVLKIVTEETTIPPFSDKYRMSFPETVDEILDVSEDEGTAHVTVMEEELVPPKPRTPEPKEQEGKREMLEFVTVTTLEQMEESAAVPQVPCVPPETVVTIAEESEQMGPVETEAEQGGLLPAPSVSPQEPSKEFDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHHGLRVVIPPRACAAPTRITCRLVKPQKLPAPPPLAEEEGLASRIIALGPAGAQFLSPVIVEIPHFASYGRGDRELVVLRSENGSVWKEHRNRYEESYMDQLLSGMDEELESQEELEKKRVCRIITTDFPLYFVVMSRICQDCDMIGPEGGCLKSTLVPMVQATFPDTAVTKKVRLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWKDNPRDSGEGDTTSLRLLCSVIGGTAQAQWEDITGTTKLVYEKECANFTTNVSARFWLADCPRTAEAVHFATVLYKELTAVPYMAKFVVFAKMNDGREGRLRCYCMTDDKVDKTLEHHENFTEVARSRDIEVVEGMPLHVELSGNLVPVKKATQSRTFFFQSFRESRLAIPIKVRDSSREASGSLSFLRKAMKYEDLQHVLCHLNISIPPCTKGSSTEERRRTLTPLSLRSLSSTDRADQKMIDIAEQLGLSWAELARELQFGVDDINRIRVENPNSLLEQSIALLNLWVSREGKGVKIENLYTALRNIDRSEIISTLEGSGRQSRSMKGSWRYMDRDYSLSPSQMNGYASLQDELLSPASLHYMLPSPLRADQYWNEVAIMDAIPMAATEQDALMEMSDMQVWSSGLTPSLVTAEDSSLECSKAEDSDATSEGRFPGHLLTDAHVPDHMGSMDLVEDDTVDSDAMNGLIELLEQEEGQRPEGKMPASDHQLGTGEQNPESEVSFVSVQQKVQATIVPSPTVSHTVERSADRLRDWNAEGSFISCLQDLTEGSWQEGVTRRLGPAHTVAAGAQGQEQEQVLAPDVELMRVSSTEDSDWQPQHPAGGWPEEPDSRCFGQGNEVLHLPGEHVTEEQFTDEQGNIITKKVIRKVVHQLDLDTDDRKEHEDLILEGSLQEPQDLEAEADHFMYSILQRDGLGAKDLTSTPNH

Target ORF information:

RefSeq Version	XM_015297390.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ankyrin 1 (ANK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297390.2

ORF Insert Sequence:

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGAGAG GGCATGCACC AACCACTGTG GACCCCTTGC CTTCTTGTTG CAAGCAAGGA 
TCTCCTGAAG GGATGCTGGA GCAGGAGTCT GCTGTGCAGG CTGATGCTGC GACCAGCTTC 
CTGAGAGCTG CAAGATCTGG GAATCTGGAC AGAGCCTTGG ATCATCTCAG GAATGGGGTA 
GATATTAACA CCTGTAACCA GAATGGGCTG AATGCCTTGC ACCTGGCCTC CAAGGAGGGC 
CACACGAAAA TGGTGGTGGA GCTTCTGCAC AAGGAGATTG TTTTGGAGAC CACTACCAAG 
AAGGGAAACA CAGCCCTGCA CATCGCTGCC CTCGCTGGAC AGCAGGACGT GGTCCGGGAA 
CTGGTGAACT ATGGGGCCAA TGTCAATGCT CAGTCGCAGA AGGGCTTCAC ACCACTCTAC 
ATGGCAGCGC AGGAAAACCA CCTGGAAGTT GTCAAATTCC TGCTGGAAAA CGGAGCCAAC 
CAGAATGTAG CCACAGAGGA TGGCTTCACA CCACTAGCTG TGGCTCTGCA GCAGGGTCAT 
GAGAATGTGG TTGCTCACCT TATCAACTAT GGGACGAAGG GTAAGGTCCG CCTGCCTGCC 
TTGCACATTG CAGCTCGCAA CGATGACACC CGTACAGCCG CCGTGCTGCT GCAGAATGAC 
CCCAATGCTG ACGTCCTCTC CAAGACTGGA TTTACACCTT TGCACATTGC AGCCCACTAT 
GAGAATCTCA GCGTGGCCCA ATTATTGCTG AATCGTGGAG CCAGCGTCAA CTTCACACCC 
CAGAATGGGA TCACTCCTTT GCACATAGCC TCTCGTCGAG GCAACATCAT CATGGTACGG 
CTGCTGCTGG ACCGTGGGGC CCAGATAGAG ACAAGGACCA AGGATGAGTT GACGCCTCTC 
CACTGTGCAG CTCGCAATGG ACATGTGCGA ATTGCAGAGA TCCTACTGGA CCATGGTGCT 
CCCATTCAAG CCAAAACAAA GAACGGTCTG TCACCGATCC ACATGGCAGC ACAGGGTGAC 
CACCTGGACT GTGTGCGCCT ACTCCTGCAG TACAGTGCTG AGATAGACGA CATCACCCTG 
GACCACCTGA CACCACTGCA TGTAGCTGCG CACTGTGGCC ACCACCGTGT GGCCAAGCTG 
CTGGTGGAGA AGGGAGCCAA GCCCAACTCC AGAGCTCTGA ATGGCTTCAC GCCTCTCCAC 
ATTGCCTGCA AGAAGAACCA CATTCGGGTG ATGGAGTTGC TTCTGAAGAC AGGAGCCTCC 
ATTGATGCTG TCACAGAGTC TGGCCTGACC CCCTTGCATG TGGCTGCCTT CATGGGGCAC 
TTGCCCATTG TCAAAACCTT GCTGCAGCGT GGAGCATCTC CTAATGTGTC CAATGTGAAA 
GTGGAGACTC CCCTGCACAT GGCAGCCAGA GCTGGGCACA TGGATGTGGC CAAGTATCTG 
ATACAGAACA AGGCCAAAAT CAACGCCAAG GCCAAGGATG ACCAGACTCC TCTGCACTGT 
GCTGCACGCA TTGGCCACAC CAGCATGGTC CAACTCCTGC TGGAGAACAA TGCCGACCCC 
AACCTGGCCA CTACAGCAGG GCACACGCCT CTGCACATCG CTGCCAGAGA GGGGCATGTA 
GACACAGCCT TGGCCCTGCT AGAGAAGGGA GCCTCTCAGA CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TCCACGTTGC AGCCAAGTAT GGGAAGGTGG ATGTGGCAGA GCTGCTGCTG 
GTGCATGATG CTCACCCCAA TGCAGCGGGG AAGAACGGCC TGACCCCACT GCACGTAGCT 
GTGCACCACA ACAACCTGGA GATCGTCAAG CTTCTGCTTC CCAAGGGGAG CTCTCCACAC 
AGCTCAGCCT GGAACGGGTA CACCCCTCTG CACATTGCCG CCAAGCAGAA CCAGATGGAG 
GTGGCCAGCA GCTTGCTGCA GTATGGTGCT TCTGCAAATG CGGAGTCTGT TCAGGGAGTC 
ACCCCTCTGC ACCTGGCTTC TCAGGAAGGG CATACAGACA TGGTAGCACT GCTTTTCTCC 
AAGCAAGCCA ATGGAAACTT AGGCAACAAG AGTGGTCTGA CTCCTCTCCA TCTCGTGGCC 
CAAGAGGGAC ACGTACCAGT GGCTGATGTT CTGGTGAAAC ATGGAGTCAC AGTGGATGCG 
ACAACCAGGA TGGGCTACAC CTCACTGCAC ATAGCCAGCC ACTATGGGAA CATCAAGTTG 
GTGAAGTTTC TGCTGCAGCA CCAGGCTGAT GTCAATGCTA AGACTAAGCT GGGCTACACA 
CCCCTGCACC AGGCGGCACA GCAGGGCCAC ACGGATGTGG TGACCCTGCT GCTGAAGCAT 
GGTGCCTCTC CCAACGAGAT CAGCACAAAT GGCACCACTC CCCTGGCCAT TGCGAAGCGC 
CTCGGTTACA TTTCCGTCAC AGATGTGCTC AAGATAGTCA CAGAGGAGAC CACCATCCCA 
CCATTCAGTG ACAAGTACCG CATGAGCTTC CCGGAGACTG TGGATGAGAT TCTGGATGTG 
TCGGAGGATG AAGGCACTGC TCATGTCACA GTAATGGAGG AGGAGCTGGT CCCACCGAAA 
CCCAGAACAC CTGAACCCAA GGAGCAGGAG GGCAAGAGGG AGATGCTGGA GTTTGTGACC 
GTGACAACAC TGGAGCAAAT GGAGGAATCT GCAGCTGTCC CACAGGTCCC CTGCGTGCCC 
CCTGAGACTG TGGTGACCAT AGCAGAGGAG TCTGAGCAGA TGGGACCTGT GGAGACTGAG 
GCTGAGCAAG GCGGCCTGCT GCCTGCACCC TCGGTGTCCC CACAGGAGCC CTCCAAGGAG 
TTCGATGAGG ACTCCTTGAT TCCCAGCAGC CCGGCCACCG AAACCTCGGA CAACATCAGC 
CCCGTGGCCA GCCCCGTTCA CACAGGGTTC CTGGTGAGCT TCATGGTGGA TGCGCGTGGC 
GGCTCCATGC GGGGCAGCCG GCACCACGGG CTGCGCGTGG TCATCCCTCC CCGTGCCTGT 
GCTGCACCCA CCCGCATCAC TTGCCGCCTG GTGAAGCCTC AGAAGCTGCC TGCCCCCCCA 
CCGCTGGCTG AGGAGGAGGG CCTGGCAAGC CGCATCATTG CACTGGGGCC CGCTGGCGCC 
CAGTTCCTCA GCCCCGTCAT TGTGGAGATT CCTCACTTTG CCTCCTATGG ACGTGGGGAC 
CGTGAGCTGG TGGTGTTGCG CAGTGAGAAT GGCTCTGTCT GGAAGGAGCA CCGCAACCGC 
TACGAGGAGA GCTACATGGA CCAACTGCTC AGTGGCATGG ATGAGGAGCT GGAGAGCCAA 
GAGGAGCTGG AGAAGAAGAG GGTCTGCCGC ATCATCACCA CTGACTTTCC TCTCTACTTC 
GTGGTCATGT CCCGGATTTG CCAGGACTGT GACATGATTG GCCCCGAGGG AGGGTGCTTG 
AAAAGCACAC TGGTGCCCAT GGTGCAGGCC ACCTTCCCAG ACACCGCTGT CACCAAGAAA 
GTGAGGCTGG CTCTGCAGGC GCAGCCCGTG CCTGATGAGC TGGTGACCAA GCTGCTGGGG 
AACCAGGCAA CCTTCAGCCC CATCGTCACA GTGGAGCCAC GTCGGAGGAA GTTCCACCGC 
CCCATTGGCC TCCGCATCCC TCTGCCCCCA TCCTGGAAGG ACAATCCCCG GGACAGCGGC 
GAGGGTGACA CCACCAGCCT GCGCCTGCTC TGTTCTGTGA TAGGAGGGAC AGCACAAGCT 
CAGTGGGAAG ACATCACAGG CACCACAAAG CTGGTCTATG AAAAAGAGTG TGCTAACTTT 
ACCACCAATG TGTCTGCCAG GTTCTGGCTG GCTGACTGCC CGCGCACTGC TGAGGCTGTG 
CACTTTGCCA CCGTGCTGTA CAAGGAACTG ACGGCTGTGC CCTACATGGC CAAATTTGTG 
GTGTTTGCTA AGATGAACGA TGGACGGGAA GGCCGGCTGC GCTGCTACTG CATGACTGAT 
GACAAGGTGG ACAAGACTTT GGAGCATCAT GAGAACTTCA CTGAGGTGGC CCGCAGCAGG 
GACATTGAGG TGGTGGAAGG AATGCCTCTG CATGTCGAGC TCTCAGGGAA CCTGGTGCCC 
GTCAAGAAGG CCACGCAGTC ACGCACCTTC TTCTTCCAGT CCTTCCGGGA AAGCCGCCTC 
GCCATCCCCA TCAAGGTCCG GGACAGCAGC CGGGAAGCCA GTGGCTCCCT GTCCTTCCTG 
CGCAAGGCCA TGAAGTATGA GGACTTGCAG CATGTGCTCT GCCACCTCAA CATCAGCATT 
CCACCCTGCA CCAAGGGAAG CAGCACTGAG GAGCGAAGGA GGACACTGAC ACCTTTGTCT 
CTGCGCTCTC TGAGCAGCAC CGACAGGGCA GACCAGAAGA TGATCGACAT TGCAGAGCAG 
CTTGGCCTCA GCTGGGCAGA GCTGGCACGT GAGCTGCAGT TTGGGGTGGA TGACATCAAC 
AGGATTCGTG TGGAGAACCC TAACTCCTTG CTGGAGCAGA GCATAGCCTT GCTTAACCTC 
TGGGTCAGCC GCGAGGGCAA GGGTGTCAAA ATTGAGAACC TGTACACAGC GCTGAGGAAC 
ATTGACCGCA GCGAGATCAT CAGCACGTTG GAGGGCTCCG GCCGGCAGAG CCGCAGCATG 
AAGGGCAGCT GGCGTTACAT GGACAGGGAC TATTCACTCT CGCCCTCCCA GATGAATGGT 
TACGCTTCGC TGCAGGACGA GCTGCTCTCC CCTGCCTCCC TGCATTATAT GCTGCCATCC 
CCTCTGCGTG CTGACCAGTA CTGGAACGAG GTGGCCATCA TGGACGCCAT CCCCATGGCC 
GCCACTGAGC AGGACGCCCT GATGGAGATG TCTGACATGC AGGTGTGGTC CTCAGGGCTC 
ACCCCTTCAC TGGTGACGGC TGAGGACTCC TCTCTGGAGT GCAGCAAGGC TGAGGACTCG 
GATGCTACAA GCGAAGGTCG TTTCCCAGGG CACCTTCTGA CAGATGCACA TGTCCCAGAC 
CACATGGGCT CTATGGACTT AGTTGAGGAT GACACAGTGG ACTCAGACGC CATGAACGGG 
CTGATTGAAC TGCTAGAGCA GGAGGAGGGG CAGAGGCCAG AGGGGAAGAT GCCAGCCAGC 
GACCACCAGC TAGGGACTGG AGAGCAGAAC CCAGAGAGTG AAGTCTCTTT TGTTTCAGTT 
CAACAGAAGG TACAAGCCAC GATCGTGCCA TCGCCAACCG TGAGCCACAC AGTGGAGAGG 
AGTGCAGACA GGCTGAGGGA CTGGAATGCA GAAGGCTCCT TTATCTCCTG CCTACAGGAC 
CTGACAGAGG GCTCCTGGCA GGAGGGGGTC ACCCGACGGC TGGGTCCAGC ACACACCGTG 
GCTGCTGGGG CGCAGGGCCA GGAGCAAGAG CAGGTCCTGG CGCCGGACGT GGAGCTGATG 
CGGGTCAGCT CCACAGAGGA CAGCGACTGG CAGCCCCAGC ACCCCGCGGG CGGCTGGCCA 
GAGGAGCCTG ACAGCCGCTG CTTTGGGCAG GGCAACGAAG TCCTCCATCT CCCTGGGGAG 
CATGTGACTG AGGAGCAGTT CACAGATGAA CAAGGCAATA TAATCACCAA GAAGGTCATC 
CGGAAAGTGG TGCATCAGTT GGACCTCGAC ACGGATGACA GGAAGGAGCA TGAGGACTTG 
ATTCTGGAGG GCTCCCTGCA GGAGCCCCAA GACCTGGAGG CAGAGGCTGA TCACTTCATG 
TACTCCATCC TACAGCGGGA CGGTCTGGGG GCCAAGGATC TCACCTCAAC ACCAAACCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK1 ( XM_015297391.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152877.1 Gallus gallus ankyrin 1 (ANK1), transcript variant X3, mRNA.

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CloneID	OGa147280
Clone ID Related Accession (Same CDS sequence)	XM_015297391.2
Accession Version	XM_015297391.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ankyrin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006109.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015297391.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGGTCA GTGATGCTGG GGCTGATGCT GCGACCAGCT TCCTGAGAGC TGCAAGATCT 
GGGAATCTGG ACAGAGCCTT GGATCATCTC AGGAATGGGG TAGATATTAA CACCTGTAAC 
CAGAATGGGC TGAATGCCTT GCACCTGGCC TCCAAGGAGG GCCACACGAA AATGGTGGTG 
GAGCTTCTGC ACAAGGAGAT TGTTTTGGAG ACCACTACCA AGAAGGGAAA CACAGCCCTG 
CACATCGCTG CCCTCGCTGG ACAGCAGGAC GTGGTCCGGG AACTGGTGAA CTATGGGGCC 
AATGTCAATG CTCAGTCGCA GAAGGGCTTC ACACCACTCT ACATGGCAGC GCAGGAAAAC 
CACCTGGAAG TTGTCAAATT CCTGCTGGAA AACGGAGCCA ACCAGAATGT AGCCACAGAG 
GATGGCTTCA CACCACTAGC TGTGGCTCTG CAGCAGGGTC ATGAGAATGT GGTTGCTCAC 
CTTATCAACT ATGGGACGAA GGGTAAGGTC CGCCTGCCTG CCTTGCACAT TGCAGCTCGC 
AACGATGACA CCCGTACAGC CGCCGTGCTG CTGCAGAATG ACCCCAATGC TGACGTCCTC 
TCCAAGACTG GATTTACACC TTTGCACATT GCAGCCCACT ATGAGAATCT CAGCGTGGCC 
CAATTATTGC TGAATCGTGG AGCCAGCGTC AACTTCACAC CCCAGAATGG GATCACTCCT 
TTGCACATAG CCTCTCGTCG AGGCAACATC ATCATGGTAC GGCTGCTGCT GGACCGTGGG 
GCCCAGATAG AGACAAGGAC CAAGGATGAG TTGACGCCTC TCCACTGTGC AGCTCGCAAT 
GGACATGTGC GAATTGCAGA GATCCTACTG GACCATGGTG CTCCCATTCA AGCCAAAACA 
AAGAACGGTC TGTCACCGAT CCACATGGCA GCACAGGGTG ACCACCTGGA CTGTGTGCGC 
CTACTCCTGC AGTACAGTGC TGAGATAGAC GACATCACCC TGGACCACCT GACACCACTG 
CATGTAGCTG CGCACTGTGG CCACCACCGT GTGGCCAAGC TGCTGGTGGA GAAGGGAGCC 
AAGCCCAACT CCAGAGCTCT GAATGGCTTC ACGCCTCTCC ACATTGCCTG CAAGAAGAAC 
CACATTCGGG TGATGGAGTT GCTTCTGAAG ACAGGAGCCT CCATTGATGC TGTCACAGAG 
TCTGGCCTGA CCCCCTTGCA TGTGGCTGCC TTCATGGGGC ACTTGCCCAT TGTCAAAACC 
TTGCTGCAGC GTGGAGCATC TCCTAATGTG TCCAATGTGA AAGTGGAGAC TCCCCTGCAC 
ATGGCAGCCA GAGCTGGGCA CATGGATGTG GCCAAGTATC TGATACAGAA CAAGGCCAAA 
ATCAACGCCA AGGCCAAGGA TGACCAGACT CCTCTGCACT GTGCTGCACG CATTGGCCAC 
ACCAGCATGG TCCAACTCCT GCTGGAGAAC AATGCCGACC CCAACCTGGC CACTACAGCA 
GGGCACACGC CTCTGCACAT CGCTGCCAGA GAGGGGCATG TAGACACAGC CTTGGCCCTG 
CTAGAGAAGG GAGCCTCTCA GACCTGCATG ACCAAGAAAG GATTTACCCC TCTCCACGTT 
GCAGCCAAGT ATGGGAAGGT GGATGTGGCA GAGCTGCTGC TGGTGCATGA TGCTCACCCC 
AATGCAGCGG GGAAGAACGG CCTGACCCCA CTGCACGTAG CTGTGCACCA CAACAACCTG 
GAGATCGTCA AGCTTCTGCT TCCCAAGGGG AGCTCTCCAC ACAGCTCAGC CTGGAACGGG 
TACACCCCTC TGCACATTGC CGCCAAGCAG AACCAGATGG AGGTGGCCAG CAGCTTGCTG 
CAGTATGGTG CTTCTGCAAA TGCGGAGTCT GTTCAGGGAG TCACCCCTCT GCACCTGGCT 
TCTCAGGAAG GGCATACAGA CATGGTAGCA CTGCTTTTCT CCAAGCAAGC CAATGGAAAC 
TTAGGCAACA AGAGTGGTCT GACTCCTCTC CATCTCGTGG CCCAAGAGGG ACACGTACCA 
GTGGCTGATG TTCTGGTGAA ACATGGAGTC ACAGTGGATG CGACAACCAG GATGGGCTAC 
ACCTCACTGC ACATAGCCAG CCACTATGGG AACATCAAGT TGGTGAAGTT TCTGCTGCAG 
CACCAGGCTG ATGTCAATGC TAAGACTAAG CTGGGCTACA CACCCCTGCA CCAGGCGGCA 
CAGCAGGGCC ACACGGATGT GGTGACCCTG CTGCTGAAGC ATGGTGCCTC TCCCAACGAG 
ATCAGCACAA ATGGCACCAC TCCCCTGGCC ATTGCGAAGC GCCTCGGTTA CATTTCCGTC 
ACAGATGTGC TCAAGATAGT CACAGAGGAG ACCACCATCC CACCATTCAG TGACAAGTAC 
CGCATGAGCT TCCCGGAGAC TGTGGATGAG ATTCTGGATG TGTCGGAGGA TGAAGGCACT 
GCTCATGTCA CAGTAATGGA GGAGGAGCTG GTCCCACCGA AACCCAGAAC ACCTGAACCC 
AAGGAGCAGG AGGGCAAGAG GGAGATGCTG GAGTTTGTGA CCGTGACAAC ACTGGAGCAA 
ATGGAGGAAT CTGCAGCTGT CCCACAGGTC CCCTGCGTGC CCCCTGAGAC TGTGGTGACC 
ATAGCAGAGG AGTCTGAGCA GATGGGACCT GTGGAGACTG AGGCTGAGCA AGGCGGCCTG 
CTGCCTGCAC CCTCGGTGTC CCCACAGGAG CCCTCCAAGG AGTTCGATGA GGACTCCTTG 
ATTCCCAGCA GCCCGGCCAC CGAAACCTCG GACAACATCA GCCCCGTGGC CAGCCCCGTT 
CACACAGGGT TCCTGGTGAG CTTCATGGTG GATGCGCGTG GCGGCTCCAT GCGGGGCAGC 
CGGCACCACG GGCTGCGCGT GGTCATCCCT CCCCGTGCCT GTGCTGCACC CACCCGCATC 
ACTTGCCGCC TGGTGAAGCC TCAGAAGCTG CCTGCCCCCC CACCGCTGGC TGAGGAGGAG 
GGCCTGGCAA GCCGCATCAT TGCACTGGGG CCCGCTGGCG CCCAGTTCCT CAGCCCCGTC 
ATTGTGGAGA TTCCTCACTT TGCCTCCTAT GGACGTGGGG ACCGTGAGCT GGTGGTGTTG 
CGCAGTGAGA ATGGCTCTGT CTGGAAGGAG CACCGCAACC GCTACGAGGA GAGCTACATG 
GACCAACTGC TCAGTGGCAT GGATGAGGAG CTGGAGAGCC AAGAGGAGCT GGAGAAGAAG 
AGGGTCTGCC GCATCATCAC CACTGACTTT CCTCTCTACT TCGTGGTCAT GTCCCGGATT 
TGCCAGGACT GTGACATGAT TGGCCCCGAG GGAGGGTGCT TGAAAAGCAC ACTGGTGCCC 
ATGGTGCAGG CCACCTTCCC AGACACCGCT GTCACCAAGA AAGTGAGGCT GGCTCTGCAG 
GCGCAGCCCG TGCCTGATGA GCTGGTGACC AAGCTGCTGG GGAACCAGGC AACCTTCAGC 
CCCATCGTCA CAGTGGAGCC ACGTCGGAGG AAGTTCCACC GCCCCATTGG CCTCCGCATC 
CCTCTGCCCC CATCCTGGAA GGACAATCCC CGGGACAGCG GCGAGGGTGA CACCACCAGC 
CTGCGCCTGC TCTGTTCTGT GATAGGAGGG ACAGCACAAG CTCAGTGGGA AGACATCACA 
GGCACCACAA AGCTGGTCTA TGAAAAAGAG TGTGCTAACT TTACCACCAA TGTGTCTGCC 
AGGTTCTGGC TGGCTGACTG CCCGCGCACT GCTGAGGCTG TGCACTTTGC CACCGTGCTG 
TACAAGGAAC TGACGGCTGT GCCCTACATG GCCAAATTTG TGGTGTTTGC TAAGATGAAC 
GATGGACGGG AAGGCCGGCT GCGCTGCTAC TGCATGACTG ATGACAAGGT GGACAAGACT 
TTGGAGCATC ATGAGAACTT CACTGAGGTG GCCCGCAGCA GGGACATTGA GGTGGTGGAA 
GGAATGCCTC TGCATGTCGA GCTCTCAGGG AACCTGGTGC CCGTCAAGAA GGCCACGCAG 
TCACGCACCT TCTTCTTCCA GTCCTTCCGG GAAAGCCGCC TCGCCATCCC CATCAAGGTC 
CGGGACAGCA GCCGGGAAGC CAGTGGCTCC CTGTCCTTCC TGCGCAAGGC CATGAAGTAT 
GAGGACTTGC AGCATGTGCT CTGCCACCTC AACATCAGCA TTCCACCCTG CACCAAGGGA 
AGCAGCACTG AGGAGCGAAG GAGGACACTG ACACCTTTGT CTCTGCGCTC TCTGAGCAGC 
ACCGACAGGG CAGACCAGAA GATGATCGAC ATTGCAGAGC AGCTTGGCCT CAGCTGGGCA 
GAGCTGGCAC GTGAGCTGCA GTTTGGGGTG GATGACATCA ACAGGATTCG TGTGGAGAAC 
CCTAACTCCT TGCTGGAGCA GAGCATAGCC TTGCTTAACC TCTGGGTCAG CCGCGAGGGC 
AAGGGTGTCA AAATTGAGAA CCTGTACACA GCGCTGAGGA ACATTGACCG CAGCGAGATC 
ATCAGCACGT TGGAGGGCTC CGGCCGGCAG AGCCGCAGCA TGAAGGGCAG CTGGCGTTAC 
ATGGACAGGG ACTATTCACT CTCGCCCTCC CAGATGAATG GTTACGCTTC GCTGCAGGAC 
GAGCTGCTCT CCCCTGCCTC CCTGCATTAT ATGCTGCCAT CCCCTCTGCG TGCTGACCAG 
TACTGGAACG AGGTGGCCAT CATGGACGCC ATCCCCATGG CCGCCACTGA GCAGGACGCC 
CTGATGGAGA TGTCTGACAT GCAGGTGTGG TCCTCAGGGC TCACCCCTTC ACTGGTGACG 
GCTGAGGACT CCTCTCTGGA GTGCAGCAAG GCTGAGGACT CGGATGCTAC AAGCGAAGGT 
CGTTTCCCAG GGCACCTTCT GACAGATGCA CATGTCCCAG ACCACATGGG CTCTATGGAC 
TTAGTTGAGG ATGACACAGT GGACTCAGAC GCCATGAACG GGCTGATTGA ACTGCTAGAG 
CAGGAGGAGG GGCAGAGGCC AGAGGGGAAG ATGCCAGCCA GCGACCACCA GCTAGGGACT 
GGAGAGCAGA ACCCAGAGAG TGAAGTCTCT TTTGTTTCAG TTCAACAGAA GGTACAAGCC 
ACGATCGTGC CATCGCCAAC CGTGAGCCAC ACAGTGGAGA GGAGTGCAGA CAGGCTGAGG 
GACTGGAATG CAGAAGGCTC CTTTATCTCC TGCCTACAGG ACCTGACAGA GGGCTCCTGG 
CAGGAGGGGG TCACCCGACG GCTGGGTCCA GCACACACCG TGGCTGCTGG GGCGCAGGGC 
CAGGAGCAAG AGCAGGTCCT GGCGCCGGAC GTGGAGCTGA TGCGGGTCAG CTCCACAGAG 
GACAGCGACT GGCAGCCCCA GCACCCCGCG GGCGGCTGGC CAGAGGAGCC TGACAGCCGC 
TGCTTTGGGC AGGGCAACGA AGTCCTCCAT CTCCCTGGGG AGCATGTGAC TGAGGAGCAG 
TTCACAGATG AACAAGGCAA TATAATCACC AAGAAGGTCA TCCGGAAAGT GGTGCATCAG 
TTGGACCTCG ACACGGATGA CAGGAAGGAG CATGAGGACT TGATTCTGGA GGGCTCCCTG 
CAGGAGCCCC AAGACCTGGA GGCAGAGGCT GATCACTTCA TGTACTCCAT CCTACAGCGG 
GACGGTCTGG GGGCCAAGGA GGAGGTGCAA GTGCGTGTCC CAAAACTGGA AGTCTCCGGG 
AGCAGGATGG GGGCTCAGAT AGTGAAACGA GCCAGTCTGA AAAGGGGGAA GCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152877.1
CDS	825..6701
Translation	MAQAAKQLKKIKDIEAQALQEQKEKEESNRKRRNRSRDRKKKVSDAGADAATSFLRAARSGNLDRALDHLRNGVDINTCNQNGLNALHLASKEGHTKMVVELLHKEIVLETTTKKGNTALHIAALAGQQDVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLSVAQLLLNRGASVNFTPQNGITPLHIASRRGNIIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRIAEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYSAEIDDITLDHLTPLHVAAHCGHHRVAKLLVEKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVAAFMGHLPIVKTLLQRGASPNVSNVKVETPLHMAARAGHMDVAKYLIQNKAKINAKAKDDQTPLHCAARIGHTSMVQLLLENNADPNLATTAGHTPLHIAAREGHVDTALALLEKGASQTCMTKKGFTPLHVAAKYGKVDVAELLLVHDAHPNAAGKNGLTPLHVAVHHNNLEIVKLLLPKGSSPHSSAWNGYTPLHIAAKQNQMEVASSLLQYGASANAESVQGVTPLHLASQEGHTDMVALLFSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLVKHGVTVDATTRMGYTSLHIASHYGNIKLVKFLLQHQADVNAKTKLGYTPLHQAAQQGHTDVVTLLLKHGASPNEISTNGTTPLAIAKRLGYISVTDVLKIVTEETTIPPFSDKYRMSFPETVDEILDVSEDEGTAHVTVMEEELVPPKPRTPEPKEQEGKREMLEFVTVTTLEQMEESAAVPQVPCVPPETVVTIAEESEQMGPVETEAEQGGLLPAPSVSPQEPSKEFDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHHGLRVVIPPRACAAPTRITCRLVKPQKLPAPPPLAEEEGLASRIIALGPAGAQFLSPVIVEIPHFASYGRGDRELVVLRSENGSVWKEHRNRYEESYMDQLLSGMDEELESQEELEKKRVCRIITTDFPLYFVVMSRICQDCDMIGPEGGCLKSTLVPMVQATFPDTAVTKKVRLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWKDNPRDSGEGDTTSLRLLCSVIGGTAQAQWEDITGTTKLVYEKECANFTTNVSARFWLADCPRTAEAVHFATVLYKELTAVPYMAKFVVFAKMNDGREGRLRCYCMTDDKVDKTLEHHENFTEVARSRDIEVVEGMPLHVELSGNLVPVKKATQSRTFFFQSFRESRLAIPIKVRDSSREASGSLSFLRKAMKYEDLQHVLCHLNISIPPCTKGSSTEERRRTLTPLSLRSLSSTDRADQKMIDIAEQLGLSWAELARELQFGVDDINRIRVENPNSLLEQSIALLNLWVSREGKGVKIENLYTALRNIDRSEIISTLEGSGRQSRSMKGSWRYMDRDYSLSPSQMNGYASLQDELLSPASLHYMLPSPLRADQYWNEVAIMDAIPMAATEQDALMEMSDMQVWSSGLTPSLVTAEDSSLECSKAEDSDATSEGRFPGHLLTDAHVPDHMGSMDLVEDDTVDSDAMNGLIELLEQEEGQRPEGKMPASDHQLGTGEQNPESEVSFVSVQQKVQATIVPSPTVSHTVERSADRLRDWNAEGSFISCLQDLTEGSWQEGVTRRLGPAHTVAAGAQGQEQEQVLAPDVELMRVSSTEDSDWQPQHPAGGWPEEPDSRCFGQGNEVLHLPGEHVTEEQFTDEQGNIITKKVIRKVVHQLDLDTDDRKEHEDLILEGSLQEPQDLEAEADHFMYSILQRDGLGAKEEVQVRVPKLEVSGSRMGAQIVKRASLKRGKH

Target ORF information:

RefSeq Version	XM_015297391.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ankyrin 1 (ANK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297391.2

ORF Insert Sequence:

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGGTCA GTGATGCTGG GGCTGATGCT GCGACCAGCT TCCTGAGAGC TGCAAGATCT 
GGGAATCTGG ACAGAGCCTT GGATCATCTC AGGAATGGGG TAGATATTAA CACCTGTAAC 
CAGAATGGGC TGAATGCCTT GCACCTGGCC TCCAAGGAGG GCCACACGAA AATGGTGGTG 
GAGCTTCTGC ACAAGGAGAT TGTTTTGGAG ACCACTACCA AGAAGGGAAA CACAGCCCTG 
CACATCGCTG CCCTCGCTGG ACAGCAGGAC GTGGTCCGGG AACTGGTGAA CTATGGGGCC 
AATGTCAATG CTCAGTCGCA GAAGGGCTTC ACACCACTCT ACATGGCAGC GCAGGAAAAC 
CACCTGGAAG TTGTCAAATT CCTGCTGGAA AACGGAGCCA ACCAGAATGT AGCCACAGAG 
GATGGCTTCA CACCACTAGC TGTGGCTCTG CAGCAGGGTC ATGAGAATGT GGTTGCTCAC 
CTTATCAACT ATGGGACGAA GGGTAAGGTC CGCCTGCCTG CCTTGCACAT TGCAGCTCGC 
AACGATGACA CCCGTACAGC CGCCGTGCTG CTGCAGAATG ACCCCAATGC TGACGTCCTC 
TCCAAGACTG GATTTACACC TTTGCACATT GCAGCCCACT ATGAGAATCT CAGCGTGGCC 
CAATTATTGC TGAATCGTGG AGCCAGCGTC AACTTCACAC CCCAGAATGG GATCACTCCT 
TTGCACATAG CCTCTCGTCG AGGCAACATC ATCATGGTAC GGCTGCTGCT GGACCGTGGG 
GCCCAGATAG AGACAAGGAC CAAGGATGAG TTGACGCCTC TCCACTGTGC AGCTCGCAAT 
GGACATGTGC GAATTGCAGA GATCCTACTG GACCATGGTG CTCCCATTCA AGCCAAAACA 
AAGAACGGTC TGTCACCGAT CCACATGGCA GCACAGGGTG ACCACCTGGA CTGTGTGCGC 
CTACTCCTGC AGTACAGTGC TGAGATAGAC GACATCACCC TGGACCACCT GACACCACTG 
CATGTAGCTG CGCACTGTGG CCACCACCGT GTGGCCAAGC TGCTGGTGGA GAAGGGAGCC 
AAGCCCAACT CCAGAGCTCT GAATGGCTTC ACGCCTCTCC ACATTGCCTG CAAGAAGAAC 
CACATTCGGG TGATGGAGTT GCTTCTGAAG ACAGGAGCCT CCATTGATGC TGTCACAGAG 
TCTGGCCTGA CCCCCTTGCA TGTGGCTGCC TTCATGGGGC ACTTGCCCAT TGTCAAAACC 
TTGCTGCAGC GTGGAGCATC TCCTAATGTG TCCAATGTGA AAGTGGAGAC TCCCCTGCAC 
ATGGCAGCCA GAGCTGGGCA CATGGATGTG GCCAAGTATC TGATACAGAA CAAGGCCAAA 
ATCAACGCCA AGGCCAAGGA TGACCAGACT CCTCTGCACT GTGCTGCACG CATTGGCCAC 
ACCAGCATGG TCCAACTCCT GCTGGAGAAC AATGCCGACC CCAACCTGGC CACTACAGCA 
GGGCACACGC CTCTGCACAT CGCTGCCAGA GAGGGGCATG TAGACACAGC CTTGGCCCTG 
CTAGAGAAGG GAGCCTCTCA GACCTGCATG ACCAAGAAAG GATTTACCCC TCTCCACGTT 
GCAGCCAAGT ATGGGAAGGT GGATGTGGCA GAGCTGCTGC TGGTGCATGA TGCTCACCCC 
AATGCAGCGG GGAAGAACGG CCTGACCCCA CTGCACGTAG CTGTGCACCA CAACAACCTG 
GAGATCGTCA AGCTTCTGCT TCCCAAGGGG AGCTCTCCAC ACAGCTCAGC CTGGAACGGG 
TACACCCCTC TGCACATTGC CGCCAAGCAG AACCAGATGG AGGTGGCCAG CAGCTTGCTG 
CAGTATGGTG CTTCTGCAAA TGCGGAGTCT GTTCAGGGAG TCACCCCTCT GCACCTGGCT 
TCTCAGGAAG GGCATACAGA CATGGTAGCA CTGCTTTTCT CCAAGCAAGC CAATGGAAAC 
TTAGGCAACA AGAGTGGTCT GACTCCTCTC CATCTCGTGG CCCAAGAGGG ACACGTACCA 
GTGGCTGATG TTCTGGTGAA ACATGGAGTC ACAGTGGATG CGACAACCAG GATGGGCTAC 
ACCTCACTGC ACATAGCCAG CCACTATGGG AACATCAAGT TGGTGAAGTT TCTGCTGCAG 
CACCAGGCTG ATGTCAATGC TAAGACTAAG CTGGGCTACA CACCCCTGCA CCAGGCGGCA 
CAGCAGGGCC ACACGGATGT GGTGACCCTG CTGCTGAAGC ATGGTGCCTC TCCCAACGAG 
ATCAGCACAA ATGGCACCAC TCCCCTGGCC ATTGCGAAGC GCCTCGGTTA CATTTCCGTC 
ACAGATGTGC TCAAGATAGT CACAGAGGAG ACCACCATCC CACCATTCAG TGACAAGTAC 
CGCATGAGCT TCCCGGAGAC TGTGGATGAG ATTCTGGATG TGTCGGAGGA TGAAGGCACT 
GCTCATGTCA CAGTAATGGA GGAGGAGCTG GTCCCACCGA AACCCAGAAC ACCTGAACCC 
AAGGAGCAGG AGGGCAAGAG GGAGATGCTG GAGTTTGTGA CCGTGACAAC ACTGGAGCAA 
ATGGAGGAAT CTGCAGCTGT CCCACAGGTC CCCTGCGTGC CCCCTGAGAC TGTGGTGACC 
ATAGCAGAGG AGTCTGAGCA GATGGGACCT GTGGAGACTG AGGCTGAGCA AGGCGGCCTG 
CTGCCTGCAC CCTCGGTGTC CCCACAGGAG CCCTCCAAGG AGTTCGATGA GGACTCCTTG 
ATTCCCAGCA GCCCGGCCAC CGAAACCTCG GACAACATCA GCCCCGTGGC CAGCCCCGTT 
CACACAGGGT TCCTGGTGAG CTTCATGGTG GATGCGCGTG GCGGCTCCAT GCGGGGCAGC 
CGGCACCACG GGCTGCGCGT GGTCATCCCT CCCCGTGCCT GTGCTGCACC CACCCGCATC 
ACTTGCCGCC TGGTGAAGCC TCAGAAGCTG CCTGCCCCCC CACCGCTGGC TGAGGAGGAG 
GGCCTGGCAA GCCGCATCAT TGCACTGGGG CCCGCTGGCG CCCAGTTCCT CAGCCCCGTC 
ATTGTGGAGA TTCCTCACTT TGCCTCCTAT GGACGTGGGG ACCGTGAGCT GGTGGTGTTG 
CGCAGTGAGA ATGGCTCTGT CTGGAAGGAG CACCGCAACC GCTACGAGGA GAGCTACATG 
GACCAACTGC TCAGTGGCAT GGATGAGGAG CTGGAGAGCC AAGAGGAGCT GGAGAAGAAG 
AGGGTCTGCC GCATCATCAC CACTGACTTT CCTCTCTACT TCGTGGTCAT GTCCCGGATT 
TGCCAGGACT GTGACATGAT TGGCCCCGAG GGAGGGTGCT TGAAAAGCAC ACTGGTGCCC 
ATGGTGCAGG CCACCTTCCC AGACACCGCT GTCACCAAGA AAGTGAGGCT GGCTCTGCAG 
GCGCAGCCCG TGCCTGATGA GCTGGTGACC AAGCTGCTGG GGAACCAGGC AACCTTCAGC 
CCCATCGTCA CAGTGGAGCC ACGTCGGAGG AAGTTCCACC GCCCCATTGG CCTCCGCATC 
CCTCTGCCCC CATCCTGGAA GGACAATCCC CGGGACAGCG GCGAGGGTGA CACCACCAGC 
CTGCGCCTGC TCTGTTCTGT GATAGGAGGG ACAGCACAAG CTCAGTGGGA AGACATCACA 
GGCACCACAA AGCTGGTCTA TGAAAAAGAG TGTGCTAACT TTACCACCAA TGTGTCTGCC 
AGGTTCTGGC TGGCTGACTG CCCGCGCACT GCTGAGGCTG TGCACTTTGC CACCGTGCTG 
TACAAGGAAC TGACGGCTGT GCCCTACATG GCCAAATTTG TGGTGTTTGC TAAGATGAAC 
GATGGACGGG AAGGCCGGCT GCGCTGCTAC TGCATGACTG ATGACAAGGT GGACAAGACT 
TTGGAGCATC ATGAGAACTT CACTGAGGTG GCCCGCAGCA GGGACATTGA GGTGGTGGAA 
GGAATGCCTC TGCATGTCGA GCTCTCAGGG AACCTGGTGC CCGTCAAGAA GGCCACGCAG 
TCACGCACCT TCTTCTTCCA GTCCTTCCGG GAAAGCCGCC TCGCCATCCC CATCAAGGTC 
CGGGACAGCA GCCGGGAAGC CAGTGGCTCC CTGTCCTTCC TGCGCAAGGC CATGAAGTAT 
GAGGACTTGC AGCATGTGCT CTGCCACCTC AACATCAGCA TTCCACCCTG CACCAAGGGA 
AGCAGCACTG AGGAGCGAAG GAGGACACTG ACACCTTTGT CTCTGCGCTC TCTGAGCAGC 
ACCGACAGGG CAGACCAGAA GATGATCGAC ATTGCAGAGC AGCTTGGCCT CAGCTGGGCA 
GAGCTGGCAC GTGAGCTGCA GTTTGGGGTG GATGACATCA ACAGGATTCG TGTGGAGAAC 
CCTAACTCCT TGCTGGAGCA GAGCATAGCC TTGCTTAACC TCTGGGTCAG CCGCGAGGGC 
AAGGGTGTCA AAATTGAGAA CCTGTACACA GCGCTGAGGA ACATTGACCG CAGCGAGATC 
ATCAGCACGT TGGAGGGCTC CGGCCGGCAG AGCCGCAGCA TGAAGGGCAG CTGGCGTTAC 
ATGGACAGGG ACTATTCACT CTCGCCCTCC CAGATGAATG GTTACGCTTC GCTGCAGGAC 
GAGCTGCTCT CCCCTGCCTC CCTGCATTAT ATGCTGCCAT CCCCTCTGCG TGCTGACCAG 
TACTGGAACG AGGTGGCCAT CATGGACGCC ATCCCCATGG CCGCCACTGA GCAGGACGCC 
CTGATGGAGA TGTCTGACAT GCAGGTGTGG TCCTCAGGGC TCACCCCTTC ACTGGTGACG 
GCTGAGGACT CCTCTCTGGA GTGCAGCAAG GCTGAGGACT CGGATGCTAC AAGCGAAGGT 
CGTTTCCCAG GGCACCTTCT GACAGATGCA CATGTCCCAG ACCACATGGG CTCTATGGAC 
TTAGTTGAGG ATGACACAGT GGACTCAGAC GCCATGAACG GGCTGATTGA ACTGCTAGAG 
CAGGAGGAGG GGCAGAGGCC AGAGGGGAAG ATGCCAGCCA GCGACCACCA GCTAGGGACT 
GGAGAGCAGA ACCCAGAGAG TGAAGTCTCT TTTGTTTCAG TTCAACAGAA GGTACAAGCC 
ACGATCGTGC CATCGCCAAC CGTGAGCCAC ACAGTGGAGA GGAGTGCAGA CAGGCTGAGG 
GACTGGAATG CAGAAGGCTC CTTTATCTCC TGCCTACAGG ACCTGACAGA GGGCTCCTGG 
CAGGAGGGGG TCACCCGACG GCTGGGTCCA GCACACACCG TGGCTGCTGG GGCGCAGGGC 
CAGGAGCAAG AGCAGGTCCT GGCGCCGGAC GTGGAGCTGA TGCGGGTCAG CTCCACAGAG 
GACAGCGACT GGCAGCCCCA GCACCCCGCG GGCGGCTGGC CAGAGGAGCC TGACAGCCGC 
TGCTTTGGGC AGGGCAACGA AGTCCTCCAT CTCCCTGGGG AGCATGTGAC TGAGGAGCAG 
TTCACAGATG AACAAGGCAA TATAATCACC AAGAAGGTCA TCCGGAAAGT GGTGCATCAG 
TTGGACCTCG ACACGGATGA CAGGAAGGAG CATGAGGACT TGATTCTGGA GGGCTCCCTG 
CAGGAGCCCC AAGACCTGGA GGCAGAGGCT GATCACTTCA TGTACTCCAT CCTACAGCGG 
GACGGTCTGG GGGCCAAGGA GGAGGTGCAA GTGCGTGTCC CAAAACTGGA AGTCTCCGGG 
AGCAGGATGG GGGCTCAGAT AGTGAAACGA GCCAGTCTGA AAAGGGGGAA GCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK1 ( XM_015297393.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152879.1 Gallus gallus ankyrin 1 (ANK1), transcript variant X4, mRNA.

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CloneID	OGa147281
Clone ID Related Accession (Same CDS sequence)	XM_015297393.2
Accession Version	XM_015297393.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ankyrin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006109.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015297393.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGGCTG ATGCTGCGAC CAGCTTCCTG AGAGCTGCAA GATCTGGGAA TCTGGACAGA 
GCCTTGGATC ATCTCAGGAA TGGGGTAGAT ATTAACACCT GTAACCAGAA TGGGCTGAAT 
GCCTTGCACC TGGCCTCCAA GGAGGGCCAC ACGAAAATGG TGGTGGAGCT TCTGCACAAG 
GAGATTGTTT TGGAGACCAC TACCAAGAAG GGAAACACAG CCCTGCACAT CGCTGCCCTC 
GCTGGACAGC AGGACGTGGT CCGGGAACTG GTGAACTATG GGGCCAATGT CAATGCTCAG 
TCGCAGAAGG GCTTCACACC ACTCTACATG GCAGCGCAGG AAAACCACCT GGAAGTTGTC 
AAATTCCTGC TGGAAAACGG AGCCAACCAG AATGTAGCCA CAGAGGATGG CTTCACACCA 
CTAGCTGTGG CTCTGCAGCA GGGTCATGAG AATGTGGTTG CTCACCTTAT CAACTATGGG 
ACGAAGGGTA AGGTCCGCCT GCCTGCCTTG CACATTGCAG CTCGCAACGA TGACACCCGT 
ACAGCCGCCG TGCTGCTGCA GAATGACCCC AATGCTGACG TCCTCTCCAA GACTGGATTT 
ACACCTTTGC ACATTGCAGC CCACTATGAG AATCTCAGCG TGGCCCAATT ATTGCTGAAT 
CGTGGAGCCA GCGTCAACTT CACACCCCAG AATGGGATCA CTCCTTTGCA CATAGCCTCT 
CGTCGAGGCA ACATCATCAT GGTACGGCTG CTGCTGGACC GTGGGGCCCA GATAGAGACA 
AGGACCAAGG ATGAGTTGAC GCCTCTCCAC TGTGCAGCTC GCAATGGACA TGTGCGAATT 
GCAGAGATCC TACTGGACCA TGGTGCTCCC ATTCAAGCCA AAACAAAGAA CGGTCTGTCA 
CCGATCCACA TGGCAGCACA GGGTGACCAC CTGGACTGTG TGCGCCTACT CCTGCAGTAC 
AGTGCTGAGA TAGACGACAT CACCCTGGAC CACCTGACAC CACTGCATGT AGCTGCGCAC 
TGTGGCCACC ACCGTGTGGC CAAGCTGCTG GTGGAGAAGG GAGCCAAGCC CAACTCCAGA 
GCTCTGAATG GCTTCACGCC TCTCCACATT GCCTGCAAGA AGAACCACAT TCGGGTGATG 
GAGTTGCTTC TGAAGACAGG AGCCTCCATT GATGCTGTCA CAGAGTCTGG CCTGACCCCC 
TTGCATGTGG CTGCCTTCAT GGGGCACTTG CCCATTGTCA AAACCTTGCT GCAGCGTGGA 
GCATCTCCTA ATGTGTCCAA TGTGAAAGTG GAGACTCCCC TGCACATGGC AGCCAGAGCT 
GGGCACATGG ATGTGGCCAA GTATCTGATA CAGAACAAGG CCAAAATCAA CGCCAAGGCC 
AAGGATGACC AGACTCCTCT GCACTGTGCT GCACGCATTG GCCACACCAG CATGGTCCAA 
CTCCTGCTGG AGAACAATGC CGACCCCAAC CTGGCCACTA CAGCAGGGCA CACGCCTCTG 
CACATCGCTG CCAGAGAGGG GCATGTAGAC ACAGCCTTGG CCCTGCTAGA GAAGGGAGCC 
TCTCAGACCT GCATGACCAA GAAAGGATTT ACCCCTCTCC ACGTTGCAGC CAAGTATGGG 
AAGGTGGATG TGGCAGAGCT GCTGCTGGTG CATGATGCTC ACCCCAATGC AGCGGGGAAG 
AACGGCCTGA CCCCACTGCA CGTAGCTGTG CACCACAACA ACCTGGAGAT CGTCAAGCTT 
CTGCTTCCCA AGGGGAGCTC TCCACACAGC TCAGCCTGGA ACGGGTACAC CCCTCTGCAC 
ATTGCCGCCA AGCAGAACCA GATGGAGGTG GCCAGCAGCT TGCTGCAGTA TGGTGCTTCT 
GCAAATGCGG AGTCTGTTCA GGGAGTCACC CCTCTGCACC TGGCTTCTCA GGAAGGGCAT 
ACAGACATGG TAGCACTGCT TTTCTCCAAG CAAGCCAATG GAAACTTAGG CAACAAGAGT 
GGTCTGACTC CTCTCCATCT CGTGGCCCAA GAGGGACACG TACCAGTGGC TGATGTTCTG 
GTGAAACATG GAGTCACAGT GGATGCGACA ACCAGGATGG GCTACACCTC ACTGCACATA 
GCCAGCCACT ATGGGAACAT CAAGTTGGTG AAGTTTCTGC TGCAGCACCA GGCTGATGTC 
AATGCTAAGA CTAAGCTGGG CTACACACCC CTGCACCAGG CGGCACAGCA GGGCCACACG 
GATGTGGTGA CCCTGCTGCT GAAGCATGGT GCCTCTCCCA ACGAGATCAG CACAAATGGC 
ACCACTCCCC TGGCCATTGC GAAGCGCCTC GGTTACATTT CCGTCACAGA TGTGCTCAAG 
ATAGTCACAG AGGAGACCAC CATCCCACCA TTCAGTGACA AGTACCGCAT GAGCTTCCCG 
GAGACTGTGG ATGAGATTCT GGATGTGTCG GAGGATGAAG GCACTGCTCA TGTCACAGTA 
ATGGAGGAGG AGCTGGTCCC ACCGAAACCC AGAACACCTG AACCCAAGGA GCAGGAGGGC 
AAGAGGGAGA TGCTGGAGTT TGTGACCGTG ACAACACTGG AGCAAATGGA GGAATCTGCA 
GCTGTCCCAC AGGTCCCCTG CGTGCCCCCT GAGACTGTGG TGACCATAGC AGAGGAGTCT 
GAGCAGATGG GACCTGTGGA GACTGAGGCT GAGCAAGGCG GCCTGCTGCC TGCACCCTCG 
GTGTCCCCAC AGGAGCCCTC CAAGGAGTTC GATGAGGACT CCTTGATTCC CAGCAGCCCG 
GCCACCGAAA CCTCGGACAA CATCAGCCCC GTGGCCAGCC CCGTTCACAC AGGGTTCCTG 
GTGAGCTTCA TGGTGGATGC GCGTGGCGGC TCCATGCGGG GCAGCCGGCA CCACGGGCTG 
CGCGTGGTCA TCCCTCCCCG TGCCTGTGCT GCACCCACCC GCATCACTTG CCGCCTGGTG 
AAGCCTCAGA AGCTGCCTGC CCCCCCACCG CTGGCTGAGG AGGAGGGCCT GGCAAGCCGC 
ATCATTGCAC TGGGGCCCGC TGGCGCCCAG TTCCTCAGCC CCGTCATTGT GGAGATTCCT 
CACTTTGCCT CCTATGGACG TGGGGACCGT GAGCTGGTGG TGTTGCGCAG TGAGAATGGC 
TCTGTCTGGA AGGAGCACCG CAACCGCTAC GAGGAGAGCT ACATGGACCA ACTGCTCAGT 
GGCATGGATG AGGAGCTGGA GAGCCAAGAG GAGCTGGAGA AGAAGAGGGT CTGCCGCATC 
ATCACCACTG ACTTTCCTCT CTACTTCGTG GTCATGTCCC GGATTTGCCA GGACTGTGAC 
ATGATTGGCC CCGAGGGAGG GTGCTTGAAA AGCACACTGG TGCCCATGGT GCAGGCCACC 
TTCCCAGACA CCGCTGTCAC CAAGAAAGTG AGGCTGGCTC TGCAGGCGCA GCCCGTGCCT 
GATGAGCTGG TGACCAAGCT GCTGGGGAAC CAGGCAACCT TCAGCCCCAT CGTCACAGTG 
GAGCCACGTC GGAGGAAGTT CCACCGCCCC ATTGGCCTCC GCATCCCTCT GCCCCCATCC 
TGGAAGGACA ATCCCCGGGA CAGCGGCGAG GGTGACACCA CCAGCCTGCG CCTGCTCTGT 
TCTGTGATAG GAGGGACAGC ACAAGCTCAG TGGGAAGACA TCACAGGCAC CACAAAGCTG 
GTCTATGAAA AAGAGTGTGC TAACTTTACC ACCAATGTGT CTGCCAGGTT CTGGCTGGCT 
GACTGCCCGC GCACTGCTGA GGCTGTGCAC TTTGCCACCG TGCTGTACAA GGAACTGACG 
GCTGTGCCCT ACATGGCCAA ATTTGTGGTG TTTGCTAAGA TGAACGATGG ACGGGAAGGC 
CGGCTGCGCT GCTACTGCAT GACTGATGAC AAGGTGGACA AGACTTTGGA GCATCATGAG 
AACTTCACTG AGGTGGCCCG CAGCAGGGAC ATTGAGGTGG TGGAAGGAAT GCCTCTGCAT 
GTCGAGCTCT CAGGGAACCT GGTGCCCGTC AAGAAGGCCA CGCAGTCACG CACCTTCTTC 
TTCCAGTCCT TCCGGGAAAG CCGCCTCGCC ATCCCCATCA AGGTCCGGGA CAGCAGCCGG 
GAAGCCAGTG GCTCCCTGTC CTTCCTGCGC AAGGCCATGA AGTATGAGGA CTTGCAGCAT 
GTGCTCTGCC ACCTCAACAT CAGCATTCCA CCCTGCACCA AGGGAAGCAG CACTGAGGAG 
CGAAGGAGGA CACTGACACC TTTGTCTCTG CGCTCTCTGA GCAGCACCGA CAGGGCAGAC 
CAGAAGATGA TCGACATTGC AGAGCAGCTT GGCCTCAGCT GGGCAGAGCT GGCACGTGAG 
CTGCAGTTTG GGGTGGATGA CATCAACAGG ATTCGTGTGG AGAACCCTAA CTCCTTGCTG 
GAGCAGAGCA TAGCCTTGCT TAACCTCTGG GTCAGCCGCG AGGGCAAGGG TGTCAAAATT 
GAGAACCTGT ACACAGCGCT GAGGAACATT GACCGCAGCG AGATCATCAG CACGTTGGAG 
GGCTCCGGCC GGCAGAGCCG CAGCATGAAG GGCAGCTGGC GTTACATGGA CAGGGACTAT 
TCACTCTCGC CCTCCCAGAT GAATGGTTAC GCTTCGCTGC AGGACGAGCT GCTCTCCCCT 
GCCTCCCTGC ATTATATGCT GCCATCCCCT CTGCGTGCTG ACCAGTACTG GAACGAGGTG 
GCCATCATGG ACGCCATCCC CATGGCCGCC ACTGAGCAGG ACGCCCTGAT GGAGATGTCT 
GACATGCAGG TGTGGTCCTC AGGGCTCACC CCTTCACTGG TGACGGCTGA GGACTCCTCT 
CTGGAGTGCA GCAAGGCTGA GGACTCGGAT GCTACAAGCG AAGGTCGTTT CCCAGGGCAC 
CTTCTGACAG ATGCACATGT CCCAGACCAC ATGGGCTCTA TGGACTTAGT TGAGGATGAC 
ACAGTGGACT CAGACGCCAT GAACGGGCTG ATTGAACTGC TAGAGCAGGA GGAGGGGCAG 
AGGCCAGAGG GGAAGATGCC AGCCAGCGAC CACCAGCTAG GGACTGGAGA GCAGAACCCA 
GAGAGTGAAG TCTCTTTTGT TTCAGTTCAA CAGAAGGTAC AAGCCACGAT CGTGCCATCG 
CCAACCGTGA GCCACACAGT GGAGAGGAGT GCAGACAGGC TGAGGGACTG GAATGCAGAA 
GGCTCCTTTA TCTCCTGCCT ACAGGACCTG ACAGAGGGCT CCTGGCAGGA GGGGGTCACC 
CGACGGCTGG GTCCAGCACA CACCGTGGCT GCTGGGGCGC AGGGCCAGGA GCAAGAGCAG 
GTCCTGGCGC CGGACGTGGA GCTGATGCGG GTCAGCTCCA CAGAGGACAG CGACTGGCAG 
CCCCAGCACC CCGCGGGCGG CTGGCCAGAG GAGCCTGACA GCCGCTGCTT TGGGCAGGGC 
AACGAAGTCC TCCATCTCCC TGGGGAGCAT GTGACTGAGG AGCAGTTCAC AGATGAACAA 
GGCAATATAA TCACCAAGAA GGTCATCCGG AAAGTGGTGC ATCAGTTGGA CCTCGACACG 
GATGACAGGA AGGAGCATGA GGACTTGATT CTGGAGGGCT CCCTGCAGGA GCCCCAAGAC 
CTGGAGGCAG AGGCTGATCA CTTCATGTAC TCCATCCTAC AGCGGGACGG TCTGGGGGCC 
AAGGAGGAGG TGCAAGTGCG TGTCCCAAAA CTGGAAGTCT CCGGGAGCAG GATGGGGGCT 
CAGATAGTGA AACGAGCCAG TCTGAAAAGG GGGAAGCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152879.1
CDS	1..5862
Translation	MAQAAKQLKKIKDIEAQALQEQKEKEESNRKRRNRSRDRKKKADAATSFLRAARSGNLDRALDHLRNGVDINTCNQNGLNALHLASKEGHTKMVVELLHKEIVLETTTKKGNTALHIAALAGQQDVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLSVAQLLLNRGASVNFTPQNGITPLHIASRRGNIIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRIAEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYSAEIDDITLDHLTPLHVAAHCGHHRVAKLLVEKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVAAFMGHLPIVKTLLQRGASPNVSNVKVETPLHMAARAGHMDVAKYLIQNKAKINAKAKDDQTPLHCAARIGHTSMVQLLLENNADPNLATTAGHTPLHIAAREGHVDTALALLEKGASQTCMTKKGFTPLHVAAKYGKVDVAELLLVHDAHPNAAGKNGLTPLHVAVHHNNLEIVKLLLPKGSSPHSSAWNGYTPLHIAAKQNQMEVASSLLQYGASANAESVQGVTPLHLASQEGHTDMVALLFSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLVKHGVTVDATTRMGYTSLHIASHYGNIKLVKFLLQHQADVNAKTKLGYTPLHQAAQQGHTDVVTLLLKHGASPNEISTNGTTPLAIAKRLGYISVTDVLKIVTEETTIPPFSDKYRMSFPETVDEILDVSEDEGTAHVTVMEEELVPPKPRTPEPKEQEGKREMLEFVTVTTLEQMEESAAVPQVPCVPPETVVTIAEESEQMGPVETEAEQGGLLPAPSVSPQEPSKEFDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHHGLRVVIPPRACAAPTRITCRLVKPQKLPAPPPLAEEEGLASRIIALGPAGAQFLSPVIVEIPHFASYGRGDRELVVLRSENGSVWKEHRNRYEESYMDQLLSGMDEELESQEELEKKRVCRIITTDFPLYFVVMSRICQDCDMIGPEGGCLKSTLVPMVQATFPDTAVTKKVRLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWKDNPRDSGEGDTTSLRLLCSVIGGTAQAQWEDITGTTKLVYEKECANFTTNVSARFWLADCPRTAEAVHFATVLYKELTAVPYMAKFVVFAKMNDGREGRLRCYCMTDDKVDKTLEHHENFTEVARSRDIEVVEGMPLHVELSGNLVPVKKATQSRTFFFQSFRESRLAIPIKVRDSSREASGSLSFLRKAMKYEDLQHVLCHLNISIPPCTKGSSTEERRRTLTPLSLRSLSSTDRADQKMIDIAEQLGLSWAELARELQFGVDDINRIRVENPNSLLEQSIALLNLWVSREGKGVKIENLYTALRNIDRSEIISTLEGSGRQSRSMKGSWRYMDRDYSLSPSQMNGYASLQDELLSPASLHYMLPSPLRADQYWNEVAIMDAIPMAATEQDALMEMSDMQVWSSGLTPSLVTAEDSSLECSKAEDSDATSEGRFPGHLLTDAHVPDHMGSMDLVEDDTVDSDAMNGLIELLEQEEGQRPEGKMPASDHQLGTGEQNPESEVSFVSVQQKVQATIVPSPTVSHTVERSADRLRDWNAEGSFISCLQDLTEGSWQEGVTRRLGPAHTVAAGAQGQEQEQVLAPDVELMRVSSTEDSDWQPQHPAGGWPEEPDSRCFGQGNEVLHLPGEHVTEEQFTDEQGNIITKKVIRKVVHQLDLDTDDRKEHEDLILEGSLQEPQDLEAEADHFMYSILQRDGLGAKEEVQVRVPKLEVSGSRMGAQIVKRASLKRGKH

Target ORF information:

RefSeq Version	XM_015297393.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ankyrin 1 (ANK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297393.2

ORF Insert Sequence:

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGGCTG ATGCTGCGAC CAGCTTCCTG AGAGCTGCAA GATCTGGGAA TCTGGACAGA 
GCCTTGGATC ATCTCAGGAA TGGGGTAGAT ATTAACACCT GTAACCAGAA TGGGCTGAAT 
GCCTTGCACC TGGCCTCCAA GGAGGGCCAC ACGAAAATGG TGGTGGAGCT TCTGCACAAG 
GAGATTGTTT TGGAGACCAC TACCAAGAAG GGAAACACAG CCCTGCACAT CGCTGCCCTC 
GCTGGACAGC AGGACGTGGT CCGGGAACTG GTGAACTATG GGGCCAATGT CAATGCTCAG 
TCGCAGAAGG GCTTCACACC ACTCTACATG GCAGCGCAGG AAAACCACCT GGAAGTTGTC 
AAATTCCTGC TGGAAAACGG AGCCAACCAG AATGTAGCCA CAGAGGATGG CTTCACACCA 
CTAGCTGTGG CTCTGCAGCA GGGTCATGAG AATGTGGTTG CTCACCTTAT CAACTATGGG 
ACGAAGGGTA AGGTCCGCCT GCCTGCCTTG CACATTGCAG CTCGCAACGA TGACACCCGT 
ACAGCCGCCG TGCTGCTGCA GAATGACCCC AATGCTGACG TCCTCTCCAA GACTGGATTT 
ACACCTTTGC ACATTGCAGC CCACTATGAG AATCTCAGCG TGGCCCAATT ATTGCTGAAT 
CGTGGAGCCA GCGTCAACTT CACACCCCAG AATGGGATCA CTCCTTTGCA CATAGCCTCT 
CGTCGAGGCA ACATCATCAT GGTACGGCTG CTGCTGGACC GTGGGGCCCA GATAGAGACA 
AGGACCAAGG ATGAGTTGAC GCCTCTCCAC TGTGCAGCTC GCAATGGACA TGTGCGAATT 
GCAGAGATCC TACTGGACCA TGGTGCTCCC ATTCAAGCCA AAACAAAGAA CGGTCTGTCA 
CCGATCCACA TGGCAGCACA GGGTGACCAC CTGGACTGTG TGCGCCTACT CCTGCAGTAC 
AGTGCTGAGA TAGACGACAT CACCCTGGAC CACCTGACAC CACTGCATGT AGCTGCGCAC 
TGTGGCCACC ACCGTGTGGC CAAGCTGCTG GTGGAGAAGG GAGCCAAGCC CAACTCCAGA 
GCTCTGAATG GCTTCACGCC TCTCCACATT GCCTGCAAGA AGAACCACAT TCGGGTGATG 
GAGTTGCTTC TGAAGACAGG AGCCTCCATT GATGCTGTCA CAGAGTCTGG CCTGACCCCC 
TTGCATGTGG CTGCCTTCAT GGGGCACTTG CCCATTGTCA AAACCTTGCT GCAGCGTGGA 
GCATCTCCTA ATGTGTCCAA TGTGAAAGTG GAGACTCCCC TGCACATGGC AGCCAGAGCT 
GGGCACATGG ATGTGGCCAA GTATCTGATA CAGAACAAGG CCAAAATCAA CGCCAAGGCC 
AAGGATGACC AGACTCCTCT GCACTGTGCT GCACGCATTG GCCACACCAG CATGGTCCAA 
CTCCTGCTGG AGAACAATGC CGACCCCAAC CTGGCCACTA CAGCAGGGCA CACGCCTCTG 
CACATCGCTG CCAGAGAGGG GCATGTAGAC ACAGCCTTGG CCCTGCTAGA GAAGGGAGCC 
TCTCAGACCT GCATGACCAA GAAAGGATTT ACCCCTCTCC ACGTTGCAGC CAAGTATGGG 
AAGGTGGATG TGGCAGAGCT GCTGCTGGTG CATGATGCTC ACCCCAATGC AGCGGGGAAG 
AACGGCCTGA CCCCACTGCA CGTAGCTGTG CACCACAACA ACCTGGAGAT CGTCAAGCTT 
CTGCTTCCCA AGGGGAGCTC TCCACACAGC TCAGCCTGGA ACGGGTACAC CCCTCTGCAC 
ATTGCCGCCA AGCAGAACCA GATGGAGGTG GCCAGCAGCT TGCTGCAGTA TGGTGCTTCT 
GCAAATGCGG AGTCTGTTCA GGGAGTCACC CCTCTGCACC TGGCTTCTCA GGAAGGGCAT 
ACAGACATGG TAGCACTGCT TTTCTCCAAG CAAGCCAATG GAAACTTAGG CAACAAGAGT 
GGTCTGACTC CTCTCCATCT CGTGGCCCAA GAGGGACACG TACCAGTGGC TGATGTTCTG 
GTGAAACATG GAGTCACAGT GGATGCGACA ACCAGGATGG GCTACACCTC ACTGCACATA 
GCCAGCCACT ATGGGAACAT CAAGTTGGTG AAGTTTCTGC TGCAGCACCA GGCTGATGTC 
AATGCTAAGA CTAAGCTGGG CTACACACCC CTGCACCAGG CGGCACAGCA GGGCCACACG 
GATGTGGTGA CCCTGCTGCT GAAGCATGGT GCCTCTCCCA ACGAGATCAG CACAAATGGC 
ACCACTCCCC TGGCCATTGC GAAGCGCCTC GGTTACATTT CCGTCACAGA TGTGCTCAAG 
ATAGTCACAG AGGAGACCAC CATCCCACCA TTCAGTGACA AGTACCGCAT GAGCTTCCCG 
GAGACTGTGG ATGAGATTCT GGATGTGTCG GAGGATGAAG GCACTGCTCA TGTCACAGTA 
ATGGAGGAGG AGCTGGTCCC ACCGAAACCC AGAACACCTG AACCCAAGGA GCAGGAGGGC 
AAGAGGGAGA TGCTGGAGTT TGTGACCGTG ACAACACTGG AGCAAATGGA GGAATCTGCA 
GCTGTCCCAC AGGTCCCCTG CGTGCCCCCT GAGACTGTGG TGACCATAGC AGAGGAGTCT 
GAGCAGATGG GACCTGTGGA GACTGAGGCT GAGCAAGGCG GCCTGCTGCC TGCACCCTCG 
GTGTCCCCAC AGGAGCCCTC CAAGGAGTTC GATGAGGACT CCTTGATTCC CAGCAGCCCG 
GCCACCGAAA CCTCGGACAA CATCAGCCCC GTGGCCAGCC CCGTTCACAC AGGGTTCCTG 
GTGAGCTTCA TGGTGGATGC GCGTGGCGGC TCCATGCGGG GCAGCCGGCA CCACGGGCTG 
CGCGTGGTCA TCCCTCCCCG TGCCTGTGCT GCACCCACCC GCATCACTTG CCGCCTGGTG 
AAGCCTCAGA AGCTGCCTGC CCCCCCACCG CTGGCTGAGG AGGAGGGCCT GGCAAGCCGC 
ATCATTGCAC TGGGGCCCGC TGGCGCCCAG TTCCTCAGCC CCGTCATTGT GGAGATTCCT 
CACTTTGCCT CCTATGGACG TGGGGACCGT GAGCTGGTGG TGTTGCGCAG TGAGAATGGC 
TCTGTCTGGA AGGAGCACCG CAACCGCTAC GAGGAGAGCT ACATGGACCA ACTGCTCAGT 
GGCATGGATG AGGAGCTGGA GAGCCAAGAG GAGCTGGAGA AGAAGAGGGT CTGCCGCATC 
ATCACCACTG ACTTTCCTCT CTACTTCGTG GTCATGTCCC GGATTTGCCA GGACTGTGAC 
ATGATTGGCC CCGAGGGAGG GTGCTTGAAA AGCACACTGG TGCCCATGGT GCAGGCCACC 
TTCCCAGACA CCGCTGTCAC CAAGAAAGTG AGGCTGGCTC TGCAGGCGCA GCCCGTGCCT 
GATGAGCTGG TGACCAAGCT GCTGGGGAAC CAGGCAACCT TCAGCCCCAT CGTCACAGTG 
GAGCCACGTC GGAGGAAGTT CCACCGCCCC ATTGGCCTCC GCATCCCTCT GCCCCCATCC 
TGGAAGGACA ATCCCCGGGA CAGCGGCGAG GGTGACACCA CCAGCCTGCG CCTGCTCTGT 
TCTGTGATAG GAGGGACAGC ACAAGCTCAG TGGGAAGACA TCACAGGCAC CACAAAGCTG 
GTCTATGAAA AAGAGTGTGC TAACTTTACC ACCAATGTGT CTGCCAGGTT CTGGCTGGCT 
GACTGCCCGC GCACTGCTGA GGCTGTGCAC TTTGCCACCG TGCTGTACAA GGAACTGACG 
GCTGTGCCCT ACATGGCCAA ATTTGTGGTG TTTGCTAAGA TGAACGATGG ACGGGAAGGC 
CGGCTGCGCT GCTACTGCAT GACTGATGAC AAGGTGGACA AGACTTTGGA GCATCATGAG 
AACTTCACTG AGGTGGCCCG CAGCAGGGAC ATTGAGGTGG TGGAAGGAAT GCCTCTGCAT 
GTCGAGCTCT CAGGGAACCT GGTGCCCGTC AAGAAGGCCA CGCAGTCACG CACCTTCTTC 
TTCCAGTCCT TCCGGGAAAG CCGCCTCGCC ATCCCCATCA AGGTCCGGGA CAGCAGCCGG 
GAAGCCAGTG GCTCCCTGTC CTTCCTGCGC AAGGCCATGA AGTATGAGGA CTTGCAGCAT 
GTGCTCTGCC ACCTCAACAT CAGCATTCCA CCCTGCACCA AGGGAAGCAG CACTGAGGAG 
CGAAGGAGGA CACTGACACC TTTGTCTCTG CGCTCTCTGA GCAGCACCGA CAGGGCAGAC 
CAGAAGATGA TCGACATTGC AGAGCAGCTT GGCCTCAGCT GGGCAGAGCT GGCACGTGAG 
CTGCAGTTTG GGGTGGATGA CATCAACAGG ATTCGTGTGG AGAACCCTAA CTCCTTGCTG 
GAGCAGAGCA TAGCCTTGCT TAACCTCTGG GTCAGCCGCG AGGGCAAGGG TGTCAAAATT 
GAGAACCTGT ACACAGCGCT GAGGAACATT GACCGCAGCG AGATCATCAG CACGTTGGAG 
GGCTCCGGCC GGCAGAGCCG CAGCATGAAG GGCAGCTGGC GTTACATGGA CAGGGACTAT 
TCACTCTCGC CCTCCCAGAT GAATGGTTAC GCTTCGCTGC AGGACGAGCT GCTCTCCCCT 
GCCTCCCTGC ATTATATGCT GCCATCCCCT CTGCGTGCTG ACCAGTACTG GAACGAGGTG 
GCCATCATGG ACGCCATCCC CATGGCCGCC ACTGAGCAGG ACGCCCTGAT GGAGATGTCT 
GACATGCAGG TGTGGTCCTC AGGGCTCACC CCTTCACTGG TGACGGCTGA GGACTCCTCT 
CTGGAGTGCA GCAAGGCTGA GGACTCGGAT GCTACAAGCG AAGGTCGTTT CCCAGGGCAC 
CTTCTGACAG ATGCACATGT CCCAGACCAC ATGGGCTCTA TGGACTTAGT TGAGGATGAC 
ACAGTGGACT CAGACGCCAT GAACGGGCTG ATTGAACTGC TAGAGCAGGA GGAGGGGCAG 
AGGCCAGAGG GGAAGATGCC AGCCAGCGAC CACCAGCTAG GGACTGGAGA GCAGAACCCA 
GAGAGTGAAG TCTCTTTTGT TTCAGTTCAA CAGAAGGTAC AAGCCACGAT CGTGCCATCG 
CCAACCGTGA GCCACACAGT GGAGAGGAGT GCAGACAGGC TGAGGGACTG GAATGCAGAA 
GGCTCCTTTA TCTCCTGCCT ACAGGACCTG ACAGAGGGCT CCTGGCAGGA GGGGGTCACC 
CGACGGCTGG GTCCAGCACA CACCGTGGCT GCTGGGGCGC AGGGCCAGGA GCAAGAGCAG 
GTCCTGGCGC CGGACGTGGA GCTGATGCGG GTCAGCTCCA CAGAGGACAG CGACTGGCAG 
CCCCAGCACC CCGCGGGCGG CTGGCCAGAG GAGCCTGACA GCCGCTGCTT TGGGCAGGGC 
AACGAAGTCC TCCATCTCCC TGGGGAGCAT GTGACTGAGG AGCAGTTCAC AGATGAACAA 
GGCAATATAA TCACCAAGAA GGTCATCCGG AAAGTGGTGC ATCAGTTGGA CCTCGACACG 
GATGACAGGA AGGAGCATGA GGACTTGATT CTGGAGGGCT CCCTGCAGGA GCCCCAAGAC 
CTGGAGGCAG AGGCTGATCA CTTCATGTAC TCCATCCTAC AGCGGGACGG TCTGGGGGCC 
AAGGAGGAGG TGCAAGTGCG TGTCCCAAAA CTGGAAGTCT CCGGGAGCAG GATGGGGGCT 
CAGATAGTGA AACGAGCCAG TCTGAAAAGG GGGAAGCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK1 ( XM_015297394.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152880.1 Gallus gallus ankyrin 1 (ANK1), transcript variant X5, mRNA.

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CloneID	OGa147282
Clone ID Related Accession (Same CDS sequence)	XM_015297394.2
Accession Version	XM_015297394.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ankyrin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006109.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015297394.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGGGT TCAGTCCTGC TGATAACACT TTCAGTCTGG CTGCTCACAC TTTGTTTGGC 
TGCAGCATTC AGCATCAGTT TGCTGATCAG ACCTGCAGAT CCTGGCTGCC CGGGGCTGAT 
GCTGCGACCA GCTTCCTGAG AGCTGCAAGA TCTGGGAATC TGGACAGAGC CTTGGATCAT 
CTCAGGAATG GGGTAGATAT TAACACCTGT AACCAGAATG GGCTGAATGC CTTGCACCTG 
GCCTCCAAGG AGGGCCACAC GAAAATGGTG GTGGAGCTTC TGCACAAGGA GATTGTTTTG 
GAGACCACTA CCAAGAAGGG AAACACAGCC CTGCACATCG CTGCCCTCGC TGGACAGCAG 
GACGTGGTCC GGGAACTGGT GAACTATGGG GCCAATGTCA ATGCTCAGTC GCAGAAGGGC 
TTCACACCAC TCTACATGGC AGCGCAGGAA AACCACCTGG AAGTTGTCAA ATTCCTGCTG 
GAAAACGGAG CCAACCAGAA TGTAGCCACA GAGGATGGCT TCACACCACT AGCTGTGGCT 
CTGCAGCAGG GTCATGAGAA TGTGGTTGCT CACCTTATCA ACTATGGGAC GAAGGGTAAG 
GTCCGCCTGC CTGCCTTGCA CATTGCAGCT CGCAACGATG ACACCCGTAC AGCCGCCGTG 
CTGCTGCAGA ATGACCCCAA TGCTGACGTC CTCTCCAAGA CTGGATTTAC ACCTTTGCAC 
ATTGCAGCCC ACTATGAGAA TCTCAGCGTG GCCCAATTAT TGCTGAATCG TGGAGCCAGC 
GTCAACTTCA CACCCCAGAA TGGGATCACT CCTTTGCACA TAGCCTCTCG TCGAGGCAAC 
ATCATCATGG TACGGCTGCT GCTGGACCGT GGGGCCCAGA TAGAGACAAG GACCAAGGAT 
GAGTTGACGC CTCTCCACTG TGCAGCTCGC AATGGACATG TGCGAATTGC AGAGATCCTA 
CTGGACCATG GTGCTCCCAT TCAAGCCAAA ACAAAGAACG GTCTGTCACC GATCCACATG 
GCAGCACAGG GTGACCACCT GGACTGTGTG CGCCTACTCC TGCAGTACAG TGCTGAGATA 
GACGACATCA CCCTGGACCA CCTGACACCA CTGCATGTAG CTGCGCACTG TGGCCACCAC 
CGTGTGGCCA AGCTGCTGGT GGAGAAGGGA GCCAAGCCCA ACTCCAGAGC TCTGAATGGC 
TTCACGCCTC TCCACATTGC CTGCAAGAAG AACCACATTC GGGTGATGGA GTTGCTTCTG 
AAGACAGGAG CCTCCATTGA TGCTGTCACA GAGTCTGGCC TGACCCCCTT GCATGTGGCT 
GCCTTCATGG GGCACTTGCC CATTGTCAAA ACCTTGCTGC AGCGTGGAGC ATCTCCTAAT 
GTGTCCAATG TGAAAGTGGA GACTCCCCTG CACATGGCAG CCAGAGCTGG GCACATGGAT 
GTGGCCAAGT ATCTGATACA GAACAAGGCC AAAATCAACG CCAAGGCCAA GGATGACCAG 
ACTCCTCTGC ACTGTGCTGC ACGCATTGGC CACACCAGCA TGGTCCAACT CCTGCTGGAG 
AACAATGCCG ACCCCAACCT GGCCACTACA GCAGGGCACA CGCCTCTGCA CATCGCTGCC 
AGAGAGGGGC ATGTAGACAC AGCCTTGGCC CTGCTAGAGA AGGGAGCCTC TCAGACCTGC 
ATGACCAAGA AAGGATTTAC CCCTCTCCAC GTTGCAGCCA AGTATGGGAA GGTGGATGTG 
GCAGAGCTGC TGCTGGTGCA TGATGCTCAC CCCAATGCAG CGGGGAAGAA CGGCCTGACC 
CCACTGCACG TAGCTGTGCA CCACAACAAC CTGGAGATCG TCAAGCTTCT GCTTCCCAAG 
GGGAGCTCTC CACACAGCTC AGCCTGGAAC GGGTACACCC CTCTGCACAT TGCCGCCAAG 
CAGAACCAGA TGGAGGTGGC CAGCAGCTTG CTGCAGTATG GTGCTTCTGC AAATGCGGAG 
TCTGTTCAGG GAGTCACCCC TCTGCACCTG GCTTCTCAGG AAGGGCATAC AGACATGGTA 
GCACTGCTTT TCTCCAAGCA AGCCAATGGA AACTTAGGCA ACAAGAGTGG TCTGACTCCT 
CTCCATCTCG TGGCCCAAGA GGGACACGTA CCAGTGGCTG ATGTTCTGGT GAAACATGGA 
GTCACAGTGG ATGCGACAAC CAGGATGGGC TACACCTCAC TGCACATAGC CAGCCACTAT 
GGGAACATCA AGTTGGTGAA GTTTCTGCTG CAGCACCAGG CTGATGTCAA TGCTAAGACT 
AAGCTGGGCT ACACACCCCT GCACCAGGCG GCACAGCAGG GCCACACGGA TGTGGTGACC 
CTGCTGCTGA AGCATGGTGC CTCTCCCAAC GAGATCAGCA CAAATGGCAC CACTCCCCTG 
GCCATTGCGA AGCGCCTCGG TTACATTTCC GTCACAGATG TGCTCAAGAT AGTCACAGAG 
GAGACCACCA TCCCACCATT CAGTGACAAG TACCGCATGA GCTTCCCGGA GACTGTGGAT 
GAGATTCTGG ATGTGTCGGA GGATGAAGGC ACTGCTCATG TCACAGTAAT GGAGGAGGAG 
CTGGTCCCAC CGAAACCCAG AACACCTGAA CCCAAGGAGC AGGAGGGCAA GAGGGAGATG 
CTGGAGTTTG TGACCGTGAC AACACTGGAG CAAATGGAGG AATCTGCAGC TGTCCCACAG 
GTCCCCTGCG TGCCCCCTGA GACTGTGGTG ACCATAGCAG AGGAGTCTGA GCAGATGGGA 
CCTGTGGAGA CTGAGGCTGA GCAAGGCGGC CTGCTGCCTG CACCCTCGGT GTCCCCACAG 
GAGCCCTCCA AGGAGTTCGA TGAGGACTCC TTGATTCCCA GCAGCCCGGC CACCGAAACC 
TCGGACAACA TCAGCCCCGT GGCCAGCCCC GTTCACACAG GGTTCCTGGT GAGCTTCATG 
GTGGATGCGC GTGGCGGCTC CATGCGGGGC AGCCGGCACC ACGGGCTGCG CGTGGTCATC 
CCTCCCCGTG CCTGTGCTGC ACCCACCCGC ATCACTTGCC GCCTGGTGAA GCCTCAGAAG 
CTGCCTGCCC CCCCACCGCT GGCTGAGGAG GAGGGCCTGG CAAGCCGCAT CATTGCACTG 
GGGCCCGCTG GCGCCCAGTT CCTCAGCCCC GTCATTGTGG AGATTCCTCA CTTTGCCTCC 
TATGGACGTG GGGACCGTGA GCTGGTGGTG TTGCGCAGTG AGAATGGCTC TGTCTGGAAG 
GAGCACCGCA ACCGCTACGA GGAGAGCTAC ATGGACCAAC TGCTCAGTGG CATGGATGAG 
GAGCTGGAGA GCCAAGAGGA GCTGGAGAAG AAGAGGGTCT GCCGCATCAT CACCACTGAC 
TTTCCTCTCT ACTTCGTGGT CATGTCCCGG ATTTGCCAGG ACTGTGACAT GATTGGCCCC 
GAGGGAGGGT GCTTGAAAAG CACACTGGTG CCCATGGTGC AGGCCACCTT CCCAGACACC 
GCTGTCACCA AGAAAGTGAG GCTGGCTCTG CAGGCGCAGC CCGTGCCTGA TGAGCTGGTG 
ACCAAGCTGC TGGGGAACCA GGCAACCTTC AGCCCCATCG TCACAGTGGA GCCACGTCGG 
AGGAAGTTCC ACCGCCCCAT TGGCCTCCGC ATCCCTCTGC CCCCATCCTG GAAGGACAAT 
CCCCGGGACA GCGGCGAGGG TGACACCACC AGCCTGCGCC TGCTCTGTTC TGTGATAGGA 
GGGACAGCAC AAGCTCAGTG GGAAGACATC ACAGGCACCA CAAAGCTGGT CTATGAAAAA 
GAGTGTGCTA ACTTTACCAC CAATGTGTCT GCCAGGTTCT GGCTGGCTGA CTGCCCGCGC 
ACTGCTGAGG CTGTGCACTT TGCCACCGTG CTGTACAAGG AACTGACGGC TGTGCCCTAC 
ATGGCCAAAT TTGTGGTGTT TGCTAAGATG AACGATGGAC GGGAAGGCCG GCTGCGCTGC 
TACTGCATGA CTGATGACAA GGTGGACAAG ACTTTGGAGC ATCATGAGAA CTTCACTGAG 
GTGGCCCGCA GCAGGGACAT TGAGGTGGTG GAAGGAATGC CTCTGCATGT CGAGCTCTCA 
GGGAACCTGG TGCCCGTCAA GAAGGCCACG CAGTCACGCA CCTTCTTCTT CCAGTCCTTC 
CGGGAAAGCC GCCTCGCCAT CCCCATCAAG GTCCGGGACA GCAGCCGGGA AGCCAGTGGC 
TCCCTGTCCT TCCTGCGCAA GGCCATGAAG TATGAGGACT TGCAGCATGT GCTCTGCCAC 
CTCAACATCA GCATTCCACC CTGCACCAAG GGAAGCAGCA CTGAGGAGCG AAGGAGGACA 
CTGACACCTT TGTCTCTGCG CTCTCTGAGC AGCACCGACA GGGCAGACCA GAAGATGATC 
GACATTGCAG AGCAGCTTGG CCTCAGCTGG GCAGAGCTGG CACGTGAGCT GCAGTTTGGG 
GTGGATGACA TCAACAGGAT TCGTGTGGAG AACCCTAACT CCTTGCTGGA GCAGAGCATA 
GCCTTGCTTA ACCTCTGGGT CAGCCGCGAG GGCAAGGGTG TCAAAATTGA GAACCTGTAC 
ACAGCGCTGA GGAACATTGA CCGCAGCGAG ATCATCAGCA CGTTGGAGGG CTCCGGCCGG 
CAGAGCCGCA GCATGAAGGG CAGCTGGCGT TACATGGACA GGGACTATTC ACTCTCGCCC 
TCCCAGATGA ATGGTTACGC TTCGCTGCAG GACGAGCTGC TCTCCCCTGC CTCCCTGCAT 
TATATGCTGC CATCCCCTCT GCGTGCTGAC CAGTACTGGA ACGAGGTGGC CATCATGGAC 
GCCATCCCCA TGGCCGCCAC TGAGCAGGAC GCCCTGATGG AGATGTCTGA CATGCAGGTG 
TGGTCCTCAG GGCTCACCCC TTCACTGGTG ACGGCTGAGG ACTCCTCTCT GGAGTGCAGC 
AAGGCTGAGG ACTCGGATGC TACAAGCGAA GGTCGTTTCC CAGGGCACCT TCTGACAGAT 
GCACATGTCC CAGACCACAT GGGCTCTATG GACTTAGTTG AGGATGACAC AGTGGACTCA 
GACGCCATGA ACGGGCTGAT TGAACTGCTA GAGCAGGAGG AGGGGCAGAG GCCAGAGGGG 
AAGATGCCAG CCAGCGACCA CCAGCTAGGG ACTGGAGAGC AGAACCCAGA GAGTGAAGTC 
TCTTTTGTTT CAGTTCAACA GAAGGTACAA GCCACGATCG TGCCATCGCC AACCGTGAGC 
CACACAGTGG AGAGGAGTGC AGACAGGCTG AGGGACTGGA ATGCAGAAGG CTCCTTTATC 
TCCTGCCTAC AGGACCTGAC AGAGGGCTCC TGGCAGGAGG GGGTCACCCG ACGGCTGGGT 
CCAGCACACA CCGTGGCTGC TGGGGCGCAG GGCCAGGAGC AAGAGCAGGT CCTGGCGCCG 
GACGTGGAGC TGATGCGGGT CAGCTCCACA GAGGACAGCG ACTGGCAGCC CCAGCACCCC 
GCGGGCGGCT GGCCAGAGGA GCCTGACAGC CGCTGCTTTG GGCAGGGCAA CGAAGTCCTC 
CATCTCCCTG GGGAGCATGT GACTGAGGAG CAGTTCACAG ATGAACAAGG CAATATAATC 
ACCAAGAAGG TCATCCGGAA AGTGGTGCAT CAGTTGGACC TCGACACGGA TGACAGGAAG 
GAGCATGAGG ACTTGATTCT GGAGGGCTCC CTGCAGGAGC CCCAAGACCT GGAGGCAGAG 
GCTGATCACT TCATGTACTC CATCCTACAG CGGGACGGTC TGGGGGCCAA GGAGGAGGTG 
CAAGTGCGTG TCCCAAAACT GGAAGTCTCC GGGAGCAGGA TGGGGGCTCA GATAGTGAAA 
CGAGCCAGTC TGAAAAGGGG GAAGCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152880.1
CDS	16..5865
Translation	MIGFSPADNTFSLAAHTLFGCSIQHQFADQTCRSWLPGADAATSFLRAARSGNLDRALDHLRNGVDINTCNQNGLNALHLASKEGHTKMVVELLHKEIVLETTTKKGNTALHIAALAGQQDVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLSVAQLLLNRGASVNFTPQNGITPLHIASRRGNIIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRIAEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYSAEIDDITLDHLTPLHVAAHCGHHRVAKLLVEKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVAAFMGHLPIVKTLLQRGASPNVSNVKVETPLHMAARAGHMDVAKYLIQNKAKINAKAKDDQTPLHCAARIGHTSMVQLLLENNADPNLATTAGHTPLHIAAREGHVDTALALLEKGASQTCMTKKGFTPLHVAAKYGKVDVAELLLVHDAHPNAAGKNGLTPLHVAVHHNNLEIVKLLLPKGSSPHSSAWNGYTPLHIAAKQNQMEVASSLLQYGASANAESVQGVTPLHLASQEGHTDMVALLFSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLVKHGVTVDATTRMGYTSLHIASHYGNIKLVKFLLQHQADVNAKTKLGYTPLHQAAQQGHTDVVTLLLKHGASPNEISTNGTTPLAIAKRLGYISVTDVLKIVTEETTIPPFSDKYRMSFPETVDEILDVSEDEGTAHVTVMEEELVPPKPRTPEPKEQEGKREMLEFVTVTTLEQMEESAAVPQVPCVPPETVVTIAEESEQMGPVETEAEQGGLLPAPSVSPQEPSKEFDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHHGLRVVIPPRACAAPTRITCRLVKPQKLPAPPPLAEEEGLASRIIALGPAGAQFLSPVIVEIPHFASYGRGDRELVVLRSENGSVWKEHRNRYEESYMDQLLSGMDEELESQEELEKKRVCRIITTDFPLYFVVMSRICQDCDMIGPEGGCLKSTLVPMVQATFPDTAVTKKVRLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWKDNPRDSGEGDTTSLRLLCSVIGGTAQAQWEDITGTTKLVYEKECANFTTNVSARFWLADCPRTAEAVHFATVLYKELTAVPYMAKFVVFAKMNDGREGRLRCYCMTDDKVDKTLEHHENFTEVARSRDIEVVEGMPLHVELSGNLVPVKKATQSRTFFFQSFRESRLAIPIKVRDSSREASGSLSFLRKAMKYEDLQHVLCHLNISIPPCTKGSSTEERRRTLTPLSLRSLSSTDRADQKMIDIAEQLGLSWAELARELQFGVDDINRIRVENPNSLLEQSIALLNLWVSREGKGVKIENLYTALRNIDRSEIISTLEGSGRQSRSMKGSWRYMDRDYSLSPSQMNGYASLQDELLSPASLHYMLPSPLRADQYWNEVAIMDAIPMAATEQDALMEMSDMQVWSSGLTPSLVTAEDSSLECSKAEDSDATSEGRFPGHLLTDAHVPDHMGSMDLVEDDTVDSDAMNGLIELLEQEEGQRPEGKMPASDHQLGTGEQNPESEVSFVSVQQKVQATIVPSPTVSHTVERSADRLRDWNAEGSFISCLQDLTEGSWQEGVTRRLGPAHTVAAGAQGQEQEQVLAPDVELMRVSSTEDSDWQPQHPAGGWPEEPDSRCFGQGNEVLHLPGEHVTEEQFTDEQGNIITKKVIRKVVHQLDLDTDDRKEHEDLILEGSLQEPQDLEAEADHFMYSILQRDGLGAKEEVQVRVPKLEVSGSRMGAQIVKRASLKRGKH

Target ORF information:

RefSeq Version	XM_015297394.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ankyrin 1 (ANK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297394.2

ORF Insert Sequence:

ATGATAGGGT TCAGTCCTGC TGATAACACT TTCAGTCTGG CTGCTCACAC TTTGTTTGGC 
TGCAGCATTC AGCATCAGTT TGCTGATCAG ACCTGCAGAT CCTGGCTGCC CGGGGCTGAT 
GCTGCGACCA GCTTCCTGAG AGCTGCAAGA TCTGGGAATC TGGACAGAGC CTTGGATCAT 
CTCAGGAATG GGGTAGATAT TAACACCTGT AACCAGAATG GGCTGAATGC CTTGCACCTG 
GCCTCCAAGG AGGGCCACAC GAAAATGGTG GTGGAGCTTC TGCACAAGGA GATTGTTTTG 
GAGACCACTA CCAAGAAGGG AAACACAGCC CTGCACATCG CTGCCCTCGC TGGACAGCAG 
GACGTGGTCC GGGAACTGGT GAACTATGGG GCCAATGTCA ATGCTCAGTC GCAGAAGGGC 
TTCACACCAC TCTACATGGC AGCGCAGGAA AACCACCTGG AAGTTGTCAA ATTCCTGCTG 
GAAAACGGAG CCAACCAGAA TGTAGCCACA GAGGATGGCT TCACACCACT AGCTGTGGCT 
CTGCAGCAGG GTCATGAGAA TGTGGTTGCT CACCTTATCA ACTATGGGAC GAAGGGTAAG 
GTCCGCCTGC CTGCCTTGCA CATTGCAGCT CGCAACGATG ACACCCGTAC AGCCGCCGTG 
CTGCTGCAGA ATGACCCCAA TGCTGACGTC CTCTCCAAGA CTGGATTTAC ACCTTTGCAC 
ATTGCAGCCC ACTATGAGAA TCTCAGCGTG GCCCAATTAT TGCTGAATCG TGGAGCCAGC 
GTCAACTTCA CACCCCAGAA TGGGATCACT CCTTTGCACA TAGCCTCTCG TCGAGGCAAC 
ATCATCATGG TACGGCTGCT GCTGGACCGT GGGGCCCAGA TAGAGACAAG GACCAAGGAT 
GAGTTGACGC CTCTCCACTG TGCAGCTCGC AATGGACATG TGCGAATTGC AGAGATCCTA 
CTGGACCATG GTGCTCCCAT TCAAGCCAAA ACAAAGAACG GTCTGTCACC GATCCACATG 
GCAGCACAGG GTGACCACCT GGACTGTGTG CGCCTACTCC TGCAGTACAG TGCTGAGATA 
GACGACATCA CCCTGGACCA CCTGACACCA CTGCATGTAG CTGCGCACTG TGGCCACCAC 
CGTGTGGCCA AGCTGCTGGT GGAGAAGGGA GCCAAGCCCA ACTCCAGAGC TCTGAATGGC 
TTCACGCCTC TCCACATTGC CTGCAAGAAG AACCACATTC GGGTGATGGA GTTGCTTCTG 
AAGACAGGAG CCTCCATTGA TGCTGTCACA GAGTCTGGCC TGACCCCCTT GCATGTGGCT 
GCCTTCATGG GGCACTTGCC CATTGTCAAA ACCTTGCTGC AGCGTGGAGC ATCTCCTAAT 
GTGTCCAATG TGAAAGTGGA GACTCCCCTG CACATGGCAG CCAGAGCTGG GCACATGGAT 
GTGGCCAAGT ATCTGATACA GAACAAGGCC AAAATCAACG CCAAGGCCAA GGATGACCAG 
ACTCCTCTGC ACTGTGCTGC ACGCATTGGC CACACCAGCA TGGTCCAACT CCTGCTGGAG 
AACAATGCCG ACCCCAACCT GGCCACTACA GCAGGGCACA CGCCTCTGCA CATCGCTGCC 
AGAGAGGGGC ATGTAGACAC AGCCTTGGCC CTGCTAGAGA AGGGAGCCTC TCAGACCTGC 
ATGACCAAGA AAGGATTTAC CCCTCTCCAC GTTGCAGCCA AGTATGGGAA GGTGGATGTG 
GCAGAGCTGC TGCTGGTGCA TGATGCTCAC CCCAATGCAG CGGGGAAGAA CGGCCTGACC 
CCACTGCACG TAGCTGTGCA CCACAACAAC CTGGAGATCG TCAAGCTTCT GCTTCCCAAG 
GGGAGCTCTC CACACAGCTC AGCCTGGAAC GGGTACACCC CTCTGCACAT TGCCGCCAAG 
CAGAACCAGA TGGAGGTGGC CAGCAGCTTG CTGCAGTATG GTGCTTCTGC AAATGCGGAG 
TCTGTTCAGG GAGTCACCCC TCTGCACCTG GCTTCTCAGG AAGGGCATAC AGACATGGTA 
GCACTGCTTT TCTCCAAGCA AGCCAATGGA AACTTAGGCA ACAAGAGTGG TCTGACTCCT 
CTCCATCTCG TGGCCCAAGA GGGACACGTA CCAGTGGCTG ATGTTCTGGT GAAACATGGA 
GTCACAGTGG ATGCGACAAC CAGGATGGGC TACACCTCAC TGCACATAGC CAGCCACTAT 
GGGAACATCA AGTTGGTGAA GTTTCTGCTG CAGCACCAGG CTGATGTCAA TGCTAAGACT 
AAGCTGGGCT ACACACCCCT GCACCAGGCG GCACAGCAGG GCCACACGGA TGTGGTGACC 
CTGCTGCTGA AGCATGGTGC CTCTCCCAAC GAGATCAGCA CAAATGGCAC CACTCCCCTG 
GCCATTGCGA AGCGCCTCGG TTACATTTCC GTCACAGATG TGCTCAAGAT AGTCACAGAG 
GAGACCACCA TCCCACCATT CAGTGACAAG TACCGCATGA GCTTCCCGGA GACTGTGGAT 
GAGATTCTGG ATGTGTCGGA GGATGAAGGC ACTGCTCATG TCACAGTAAT GGAGGAGGAG 
CTGGTCCCAC CGAAACCCAG AACACCTGAA CCCAAGGAGC AGGAGGGCAA GAGGGAGATG 
CTGGAGTTTG TGACCGTGAC AACACTGGAG CAAATGGAGG AATCTGCAGC TGTCCCACAG 
GTCCCCTGCG TGCCCCCTGA GACTGTGGTG ACCATAGCAG AGGAGTCTGA GCAGATGGGA 
CCTGTGGAGA CTGAGGCTGA GCAAGGCGGC CTGCTGCCTG CACCCTCGGT GTCCCCACAG 
GAGCCCTCCA AGGAGTTCGA TGAGGACTCC TTGATTCCCA GCAGCCCGGC CACCGAAACC 
TCGGACAACA TCAGCCCCGT GGCCAGCCCC GTTCACACAG GGTTCCTGGT GAGCTTCATG 
GTGGATGCGC GTGGCGGCTC CATGCGGGGC AGCCGGCACC ACGGGCTGCG CGTGGTCATC 
CCTCCCCGTG CCTGTGCTGC ACCCACCCGC ATCACTTGCC GCCTGGTGAA GCCTCAGAAG 
CTGCCTGCCC CCCCACCGCT GGCTGAGGAG GAGGGCCTGG CAAGCCGCAT CATTGCACTG 
GGGCCCGCTG GCGCCCAGTT CCTCAGCCCC GTCATTGTGG AGATTCCTCA CTTTGCCTCC 
TATGGACGTG GGGACCGTGA GCTGGTGGTG TTGCGCAGTG AGAATGGCTC TGTCTGGAAG 
GAGCACCGCA ACCGCTACGA GGAGAGCTAC ATGGACCAAC TGCTCAGTGG CATGGATGAG 
GAGCTGGAGA GCCAAGAGGA GCTGGAGAAG AAGAGGGTCT GCCGCATCAT CACCACTGAC 
TTTCCTCTCT ACTTCGTGGT CATGTCCCGG ATTTGCCAGG ACTGTGACAT GATTGGCCCC 
GAGGGAGGGT GCTTGAAAAG CACACTGGTG CCCATGGTGC AGGCCACCTT CCCAGACACC 
GCTGTCACCA AGAAAGTGAG GCTGGCTCTG CAGGCGCAGC CCGTGCCTGA TGAGCTGGTG 
ACCAAGCTGC TGGGGAACCA GGCAACCTTC AGCCCCATCG TCACAGTGGA GCCACGTCGG 
AGGAAGTTCC ACCGCCCCAT TGGCCTCCGC ATCCCTCTGC CCCCATCCTG GAAGGACAAT 
CCCCGGGACA GCGGCGAGGG TGACACCACC AGCCTGCGCC TGCTCTGTTC TGTGATAGGA 
GGGACAGCAC AAGCTCAGTG GGAAGACATC ACAGGCACCA CAAAGCTGGT CTATGAAAAA 
GAGTGTGCTA ACTTTACCAC CAATGTGTCT GCCAGGTTCT GGCTGGCTGA CTGCCCGCGC 
ACTGCTGAGG CTGTGCACTT TGCCACCGTG CTGTACAAGG AACTGACGGC TGTGCCCTAC 
ATGGCCAAAT TTGTGGTGTT TGCTAAGATG AACGATGGAC GGGAAGGCCG GCTGCGCTGC 
TACTGCATGA CTGATGACAA GGTGGACAAG ACTTTGGAGC ATCATGAGAA CTTCACTGAG 
GTGGCCCGCA GCAGGGACAT TGAGGTGGTG GAAGGAATGC CTCTGCATGT CGAGCTCTCA 
GGGAACCTGG TGCCCGTCAA GAAGGCCACG CAGTCACGCA CCTTCTTCTT CCAGTCCTTC 
CGGGAAAGCC GCCTCGCCAT CCCCATCAAG GTCCGGGACA GCAGCCGGGA AGCCAGTGGC 
TCCCTGTCCT TCCTGCGCAA GGCCATGAAG TATGAGGACT TGCAGCATGT GCTCTGCCAC 
CTCAACATCA GCATTCCACC CTGCACCAAG GGAAGCAGCA CTGAGGAGCG AAGGAGGACA 
CTGACACCTT TGTCTCTGCG CTCTCTGAGC AGCACCGACA GGGCAGACCA GAAGATGATC 
GACATTGCAG AGCAGCTTGG CCTCAGCTGG GCAGAGCTGG CACGTGAGCT GCAGTTTGGG 
GTGGATGACA TCAACAGGAT TCGTGTGGAG AACCCTAACT CCTTGCTGGA GCAGAGCATA 
GCCTTGCTTA ACCTCTGGGT CAGCCGCGAG GGCAAGGGTG TCAAAATTGA GAACCTGTAC 
ACAGCGCTGA GGAACATTGA CCGCAGCGAG ATCATCAGCA CGTTGGAGGG CTCCGGCCGG 
CAGAGCCGCA GCATGAAGGG CAGCTGGCGT TACATGGACA GGGACTATTC ACTCTCGCCC 
TCCCAGATGA ATGGTTACGC TTCGCTGCAG GACGAGCTGC TCTCCCCTGC CTCCCTGCAT 
TATATGCTGC CATCCCCTCT GCGTGCTGAC CAGTACTGGA ACGAGGTGGC CATCATGGAC 
GCCATCCCCA TGGCCGCCAC TGAGCAGGAC GCCCTGATGG AGATGTCTGA CATGCAGGTG 
TGGTCCTCAG GGCTCACCCC TTCACTGGTG ACGGCTGAGG ACTCCTCTCT GGAGTGCAGC 
AAGGCTGAGG ACTCGGATGC TACAAGCGAA GGTCGTTTCC CAGGGCACCT TCTGACAGAT 
GCACATGTCC CAGACCACAT GGGCTCTATG GACTTAGTTG AGGATGACAC AGTGGACTCA 
GACGCCATGA ACGGGCTGAT TGAACTGCTA GAGCAGGAGG AGGGGCAGAG GCCAGAGGGG 
AAGATGCCAG CCAGCGACCA CCAGCTAGGG ACTGGAGAGC AGAACCCAGA GAGTGAAGTC 
TCTTTTGTTT CAGTTCAACA GAAGGTACAA GCCACGATCG TGCCATCGCC AACCGTGAGC 
CACACAGTGG AGAGGAGTGC AGACAGGCTG AGGGACTGGA ATGCAGAAGG CTCCTTTATC 
TCCTGCCTAC AGGACCTGAC AGAGGGCTCC TGGCAGGAGG GGGTCACCCG ACGGCTGGGT 
CCAGCACACA CCGTGGCTGC TGGGGCGCAG GGCCAGGAGC AAGAGCAGGT CCTGGCGCCG 
GACGTGGAGC TGATGCGGGT CAGCTCCACA GAGGACAGCG ACTGGCAGCC CCAGCACCCC 
GCGGGCGGCT GGCCAGAGGA GCCTGACAGC CGCTGCTTTG GGCAGGGCAA CGAAGTCCTC 
CATCTCCCTG GGGAGCATGT GACTGAGGAG CAGTTCACAG ATGAACAAGG CAATATAATC 
ACCAAGAAGG TCATCCGGAA AGTGGTGCAT CAGTTGGACC TCGACACGGA TGACAGGAAG 
GAGCATGAGG ACTTGATTCT GGAGGGCTCC CTGCAGGAGC CCCAAGACCT GGAGGCAGAG 
GCTGATCACT TCATGTACTC CATCCTACAG CGGGACGGTC TGGGGGCCAA GGAGGAGGTG 
CAAGTGCGTG TCCCAAAACT GGAAGTCTCC GGGAGCAGGA TGGGGGCTCA GATAGTGAAA 
CGAGCCAGTC TGAAAAGGGG GAAGCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK1 ( XM_015297395.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152881.1 Gallus gallus ankyrin 1 (ANK1), transcript variant X6, mRNA.

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CloneID	OGa147283
Clone ID Related Accession (Same CDS sequence)	XM_015297395.2
Accession Version	XM_015297395.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ankyrin-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006109.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015297395.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAC GAAAGGGCAA GTCTGGTCCA GACGTGGCTG CTGATGCTGC GACCAGCTTC 
CTGAGAGCTG CAAGATCTGG GAATCTGGAC AGAGCCTTGG ATCATCTCAG GAATGGGGTA 
GATATTAACA CCTGTAACCA GAATGGGCTG AATGCCTTGC ACCTGGCCTC CAAGGAGGGC 
CACACGAAAA TGGTGGTGGA GCTTCTGCAC AAGGAGATTG TTTTGGAGAC CACTACCAAG 
AAGGGAAACA CAGCCCTGCA CATCGCTGCC CTCGCTGGAC AGCAGGACGT GGTCCGGGAA 
CTGGTGAACT ATGGGGCCAA TGTCAATGCT CAGTCGCAGA AGGGCTTCAC ACCACTCTAC 
ATGGCAGCGC AGGAAAACCA CCTGGAAGTT GTCAAATTCC TGCTGGAAAA CGGAGCCAAC 
CAGAATGTAG CCACAGAGGA TGGCTTCACA CCACTAGCTG TGGCTCTGCA GCAGGGTCAT 
GAGAATGTGG TTGCTCACCT TATCAACTAT GGGACGAAGG GTAAGGTCCG CCTGCCTGCC 
TTGCACATTG CAGCTCGCAA CGATGACACC CGTACAGCCG CCGTGCTGCT GCAGAATGAC 
CCCAATGCTG ACGTCCTCTC CAAGACTGGA TTTACACCTT TGCACATTGC AGCCCACTAT 
GAGAATCTCA GCGTGGCCCA ATTATTGCTG AATCGTGGAG CCAGCGTCAA CTTCACACCC 
CAGAATGGGA TCACTCCTTT GCACATAGCC TCTCGTCGAG GCAACATCAT CATGGTACGG 
CTGCTGCTGG ACCGTGGGGC CCAGATAGAG ACAAGGACCA AGGATGAGTT GACGCCTCTC 
CACTGTGCAG CTCGCAATGG ACATGTGCGA ATTGCAGAGA TCCTACTGGA CCATGGTGCT 
CCCATTCAAG CCAAAACAAA GAACGGTCTG TCACCGATCC ACATGGCAGC ACAGGGTGAC 
CACCTGGACT GTGTGCGCCT ACTCCTGCAG TACAGTGCTG AGATAGACGA CATCACCCTG 
GACCACCTGA CACCACTGCA TGTAGCTGCG CACTGTGGCC ACCACCGTGT GGCCAAGCTG 
CTGGTGGAGA AGGGAGCCAA GCCCAACTCC AGAGCTCTGA ATGGCTTCAC GCCTCTCCAC 
ATTGCCTGCA AGAAGAACCA CATTCGGGTG ATGGAGTTGC TTCTGAAGAC AGGAGCCTCC 
ATTGATGCTG TCACAGAGTC TGGCCTGACC CCCTTGCATG TGGCTGCCTT CATGGGGCAC 
TTGCCCATTG TCAAAACCTT GCTGCAGCGT GGAGCATCTC CTAATGTGTC CAATGTGAAA 
GTGGAGACTC CCCTGCACAT GGCAGCCAGA GCTGGGCACA TGGATGTGGC CAAGTATCTG 
ATACAGAACA AGGCCAAAAT CAACGCCAAG GCCAAGGATG ACCAGACTCC TCTGCACTGT 
GCTGCACGCA TTGGCCACAC CAGCATGGTC CAACTCCTGC TGGAGAACAA TGCCGACCCC 
AACCTGGCCA CTACAGCAGG GCACACGCCT CTGCACATCG CTGCCAGAGA GGGGCATGTA 
GACACAGCCT TGGCCCTGCT AGAGAAGGGA GCCTCTCAGA CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TCCACGTTGC AGCCAAGTAT GGGAAGGTGG ATGTGGCAGA GCTGCTGCTG 
GTGCATGATG CTCACCCCAA TGCAGCGGGG AAGAACGGCC TGACCCCACT GCACGTAGCT 
GTGCACCACA ACAACCTGGA GATCGTCAAG CTTCTGCTTC CCAAGGGGAG CTCTCCACAC 
AGCTCAGCCT GGAACGGGTA CACCCCTCTG CACATTGCCG CCAAGCAGAA CCAGATGGAG 
GTGGCCAGCA GCTTGCTGCA GTATGGTGCT TCTGCAAATG CGGAGTCTGT TCAGGGAGTC 
ACCCCTCTGC ACCTGGCTTC TCAGGAAGGG CATACAGACA TGGTAGCACT GCTTTTCTCC 
AAGCAAGCCA ATGGAAACTT AGGCAACAAG AGTGGTCTGA CTCCTCTCCA TCTCGTGGCC 
CAAGAGGGAC ACGTACCAGT GGCTGATGTT CTGGTGAAAC ATGGAGTCAC AGTGGATGCG 
ACAACCAGGA TGGGCTACAC CTCACTGCAC ATAGCCAGCC ACTATGGGAA CATCAAGTTG 
GTGAAGTTTC TGCTGCAGCA CCAGGCTGAT GTCAATGCTA AGACTAAGCT GGGCTACACA 
CCCCTGCACC AGGCGGCACA GCAGGGCCAC ACGGATGTGG TGACCCTGCT GCTGAAGCAT 
GGTGCCTCTC CCAACGAGAT CAGCACAAAT GGCACCACTC CCCTGGCCAT TGCGAAGCGC 
CTCGGTTACA TTTCCGTCAC AGATGTGCTC AAGATAGTCA CAGAGGAGAC CACCATCCCA 
CCATTCAGTG ACAAGTACCG CATGAGCTTC CCGGAGACTG TGGATGAGAT TCTGGATGTG 
TCGGAGGATG AAGGCACTGC TCATGTCACA GTAATGGAGG AGGAGCTGGT CCCACCGAAA 
CCCAGAACAC CTGAACCCAA GGAGCAGGAG GGCAAGAGGG AGATGCTGGA GTTTGTGACC 
GTGACAACAC TGGAGCAAAT GGAGGAATCT GCAGCTGTCC CACAGGTCCC CTGCGTGCCC 
CCTGAGACTG TGGTGACCAT AGCAGAGGAG TCTGAGCAGA TGGGACCTGT GGAGACTGAG 
GCTGAGCAAG GCGGCCTGCT GCCTGCACCC TCGGTGTCCC CACAGGAGCC CTCCAAGGAG 
TTCGATGAGG ACTCCTTGAT TCCCAGCAGC CCGGCCACCG AAACCTCGGA CAACATCAGC 
CCCGTGGCCA GCCCCGTTCA CACAGGGTTC CTGGTGAGCT TCATGGTGGA TGCGCGTGGC 
GGCTCCATGC GGGGCAGCCG GCACCACGGG CTGCGCGTGG TCATCCCTCC CCGTGCCTGT 
GCTGCACCCA CCCGCATCAC TTGCCGCCTG GTGAAGCCTC AGAAGCTGCC TGCCCCCCCA 
CCGCTGGCTG AGGAGGAGGG CCTGGCAAGC CGCATCATTG CACTGGGGCC CGCTGGCGCC 
CAGTTCCTCA GCCCCGTCAT TGTGGAGATT CCTCACTTTG CCTCCTATGG ACGTGGGGAC 
CGTGAGCTGG TGGTGTTGCG CAGTGAGAAT GGCTCTGTCT GGAAGGAGCA CCGCAACCGC 
TACGAGGAGA GCTACATGGA CCAACTGCTC AGTGGCATGG ATGAGGAGCT GGAGAGCCAA 
GAGGAGCTGG AGAAGAAGAG GGTCTGCCGC ATCATCACCA CTGACTTTCC TCTCTACTTC 
GTGGTCATGT CCCGGATTTG CCAGGACTGT GACATGATTG GCCCCGAGGG AGGGTGCTTG 
AAAAGCACAC TGGTGCCCAT GGTGCAGGCC ACCTTCCCAG ACACCGCTGT CACCAAGAAA 
GTGAGGCTGG CTCTGCAGGC GCAGCCCGTG CCTGATGAGC TGGTGACCAA GCTGCTGGGG 
AACCAGGCAA CCTTCAGCCC CATCGTCACA GTGGAGCCAC GTCGGAGGAA GTTCCACCGC 
CCCATTGGCC TCCGCATCCC TCTGCCCCCA TCCTGGAAGG ACAATCCCCG GGACAGCGGC 
GAGGGTGACA CCACCAGCCT GCGCCTGCTC TGTTCTGTGA TAGGAGGGAC AGCACAAGCT 
CAGTGGGAAG ACATCACAGG CACCACAAAG CTGGTCTATG AAAAAGAGTG TGCTAACTTT 
ACCACCAATG TGTCTGCCAG GTTCTGGCTG GCTGACTGCC CGCGCACTGC TGAGGCTGTG 
CACTTTGCCA CCGTGCTGTA CAAGGAACTG ACGGCTGTGC CCTACATGGC CAAATTTGTG 
GTGTTTGCTA AGATGAACGA TGGACGGGAA GGCCGGCTGC GCTGCTACTG CATGACTGAT 
GACAAGGTGG ACAAGACTTT GGAGCATCAT GAGAACTTCA CTGAGGTGGC CCGCAGCAGG 
GACATTGAGG TGGTGGAAGG AATGCCTCTG CATGTCGAGC TCTCAGGGAA CCTGGTGCCC 
GTCAAGAAGG CCACGCAGTC ACGCACCTTC TTCTTCCAGT CCTTCCGGGA AAGCCGCCTC 
GCCATCCCCA TCAAGGTCCG GGACAGCAGC CGGGAAGCCA GTGGCTCCCT GTCCTTCCTG 
CGCAAGGCCA TGAAGTATGA GGACTTGCAG CATGTGCTCT GCCACCTCAA CATCAGCATT 
CCACCCTGCA CCAAGGGAAG CAGCACTGAG GAGCGAAGGA GGACACTGAC ACCTTTGTCT 
CTGCGCTCTC TGAGCAGCAC CGACAGGGCA GACCAGAAGA TGATCGACAT TGCAGAGCAG 
CTTGGCCTCA GCTGGGCAGA GCTGGCACGT GAGCTGCAGT TTGGGGTGGA TGACATCAAC 
AGGATTCGTG TGGAGAACCC TAACTCCTTG CTGGAGCAGA GCATAGCCTT GCTTAACCTC 
TGGGTCAGCC GCGAGGGCAA GGGTGTCAAA ATTGAGAACC TGTACACAGC GCTGAGGAAC 
ATTGACCGCA GCGAGATCAT CAGCACGTTG GAGGGCTCCG GCCGGCAGAG CCGCAGCATG 
AAGGGCAGCT GGCGTTACAT GGACAGGGAC TATTCACTCT CGCCCTCCCA GATGAATGGT 
TACGCTTCGC TGCAGGACGA GCTGCTCTCC CCTGCCTCCC TGCATTATAT GCTGCCATCC 
CCTCTGCGTG CTGACCAGTA CTGGAACGAG GTGGCCATCA TGGACGCCAT CCCCATGGCC 
GCCACTGAGC AGGACGCCCT GATGGAGATG TCTGACATGC AGGTGTGGTC CTCAGGGCTC 
ACCCCTTCAC TGGTGACGGC TGAGGACTCC TCTCTGGAGT GCAGCAAGGC TGAGGACTCG 
GATGCTACAA GCGAAGGTCG TTTCCCAGGG CACCTTCTGA CAGATGCACA TGTCCCAGAC 
CACATGGGCT CTATGGACTT AGTTGAGGAT GACACAGTGG ACTCAGACGC CATGAACGGG 
CTGATTGAAC TGCTAGAGCA GGAGGAGGGG CAGAGGCCAG AGGGGAAGAT GCCAGCCAGC 
GACCACCAGC TAGGGACTGG AGAGCAGAAC CCAGAGAGTG AAGTCTCTTT TGTTTCAGTT 
CAACAGAAGG TACAAGCCAC GATCGTGCCA TCGCCAACCG TGAGCCACAC AGTGGAGAGG 
AGTGCAGACA GGCTGAGGGA CTGGAATGCA GAAGGCTCCT TTATCTCCTG CCTACAGGAC 
CTGACAGAGG GCTCCTGGCA GGAGGGGGTC ACCCGACGGC TGGGTCCAGC ACACACCGTG 
GCTGCTGGGG CGCAGGGCCA GGAGCAAGAG CAGGTCCTGG CGCCGGACGT GGAGCTGATG 
CGGGTCAGCT CCACAGAGGA CAGCGACTGG CAGCCCCAGC ACCCCGCGGG CGGCTGGCCA 
GAGGAGCCTG ACAGCCGCTG CTTTGGGCAG GGCAACGAAG TCCTCCATCT CCCTGGGGAG 
CATGTGACTG AGGAGCAGTT CACAGATGAA CAAGGCAATA TAATCACCAA GAAGGTCATC 
CGGAAAGTGG TGCATCAGTT GGACCTCGAC ACGGATGACA GGAAGGAGCA TGAGGACTTG 
ATTCTGGAGG GCTCCCTGCA GGAGCCCCAA GACCTGGAGG CAGAGGCTGA TCACTTCATG 
TACTCCATCC TACAGCGGGA CGGTCTGGGG GCCAAGGAGG AGGTGCAAGT GCGTGTCCCA 
AAACTGGAAG TCTCCGGGAG CAGGATGGGG GCTCAGATAG TGAAACGAGC CAGTCTGAAA 
AGGGGGAAGC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152881.1
CDS	16..5790
Translation	MAARKGKSGPDVAADAATSFLRAARSGNLDRALDHLRNGVDINTCNQNGLNALHLASKEGHTKMVVELLHKEIVLETTTKKGNTALHIAALAGQQDVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLSVAQLLLNRGASVNFTPQNGITPLHIASRRGNIIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRIAEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYSAEIDDITLDHLTPLHVAAHCGHHRVAKLLVEKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVAAFMGHLPIVKTLLQRGASPNVSNVKVETPLHMAARAGHMDVAKYLIQNKAKINAKAKDDQTPLHCAARIGHTSMVQLLLENNADPNLATTAGHTPLHIAAREGHVDTALALLEKGASQTCMTKKGFTPLHVAAKYGKVDVAELLLVHDAHPNAAGKNGLTPLHVAVHHNNLEIVKLLLPKGSSPHSSAWNGYTPLHIAAKQNQMEVASSLLQYGASANAESVQGVTPLHLASQEGHTDMVALLFSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLVKHGVTVDATTRMGYTSLHIASHYGNIKLVKFLLQHQADVNAKTKLGYTPLHQAAQQGHTDVVTLLLKHGASPNEISTNGTTPLAIAKRLGYISVTDVLKIVTEETTIPPFSDKYRMSFPETVDEILDVSEDEGTAHVTVMEEELVPPKPRTPEPKEQEGKREMLEFVTVTTLEQMEESAAVPQVPCVPPETVVTIAEESEQMGPVETEAEQGGLLPAPSVSPQEPSKEFDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHHGLRVVIPPRACAAPTRITCRLVKPQKLPAPPPLAEEEGLASRIIALGPAGAQFLSPVIVEIPHFASYGRGDRELVVLRSENGSVWKEHRNRYEESYMDQLLSGMDEELESQEELEKKRVCRIITTDFPLYFVVMSRICQDCDMIGPEGGCLKSTLVPMVQATFPDTAVTKKVRLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWKDNPRDSGEGDTTSLRLLCSVIGGTAQAQWEDITGTTKLVYEKECANFTTNVSARFWLADCPRTAEAVHFATVLYKELTAVPYMAKFVVFAKMNDGREGRLRCYCMTDDKVDKTLEHHENFTEVARSRDIEVVEGMPLHVELSGNLVPVKKATQSRTFFFQSFRESRLAIPIKVRDSSREASGSLSFLRKAMKYEDLQHVLCHLNISIPPCTKGSSTEERRRTLTPLSLRSLSSTDRADQKMIDIAEQLGLSWAELARELQFGVDDINRIRVENPNSLLEQSIALLNLWVSREGKGVKIENLYTALRNIDRSEIISTLEGSGRQSRSMKGSWRYMDRDYSLSPSQMNGYASLQDELLSPASLHYMLPSPLRADQYWNEVAIMDAIPMAATEQDALMEMSDMQVWSSGLTPSLVTAEDSSLECSKAEDSDATSEGRFPGHLLTDAHVPDHMGSMDLVEDDTVDSDAMNGLIELLEQEEGQRPEGKMPASDHQLGTGEQNPESEVSFVSVQQKVQATIVPSPTVSHTVERSADRLRDWNAEGSFISCLQDLTEGSWQEGVTRRLGPAHTVAAGAQGQEQEQVLAPDVELMRVSSTEDSDWQPQHPAGGWPEEPDSRCFGQGNEVLHLPGEHVTEEQFTDEQGNIITKKVIRKVVHQLDLDTDDRKEHEDLILEGSLQEPQDLEAEADHFMYSILQRDGLGAKEEVQVRVPKLEVSGSRMGAQIVKRASLKRGKH

Target ORF information:

RefSeq Version	XM_015297395.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ankyrin 1 (ANK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297395.2

ORF Insert Sequence:

ATGGCAGCAC GAAAGGGCAA GTCTGGTCCA GACGTGGCTG CTGATGCTGC GACCAGCTTC 
CTGAGAGCTG CAAGATCTGG GAATCTGGAC AGAGCCTTGG ATCATCTCAG GAATGGGGTA 
GATATTAACA CCTGTAACCA GAATGGGCTG AATGCCTTGC ACCTGGCCTC CAAGGAGGGC 
CACACGAAAA TGGTGGTGGA GCTTCTGCAC AAGGAGATTG TTTTGGAGAC CACTACCAAG 
AAGGGAAACA CAGCCCTGCA CATCGCTGCC CTCGCTGGAC AGCAGGACGT GGTCCGGGAA 
CTGGTGAACT ATGGGGCCAA TGTCAATGCT CAGTCGCAGA AGGGCTTCAC ACCACTCTAC 
ATGGCAGCGC AGGAAAACCA CCTGGAAGTT GTCAAATTCC TGCTGGAAAA CGGAGCCAAC 
CAGAATGTAG CCACAGAGGA TGGCTTCACA CCACTAGCTG TGGCTCTGCA GCAGGGTCAT 
GAGAATGTGG TTGCTCACCT TATCAACTAT GGGACGAAGG GTAAGGTCCG CCTGCCTGCC 
TTGCACATTG CAGCTCGCAA CGATGACACC CGTACAGCCG CCGTGCTGCT GCAGAATGAC 
CCCAATGCTG ACGTCCTCTC CAAGACTGGA TTTACACCTT TGCACATTGC AGCCCACTAT 
GAGAATCTCA GCGTGGCCCA ATTATTGCTG AATCGTGGAG CCAGCGTCAA CTTCACACCC 
CAGAATGGGA TCACTCCTTT GCACATAGCC TCTCGTCGAG GCAACATCAT CATGGTACGG 
CTGCTGCTGG ACCGTGGGGC CCAGATAGAG ACAAGGACCA AGGATGAGTT GACGCCTCTC 
CACTGTGCAG CTCGCAATGG ACATGTGCGA ATTGCAGAGA TCCTACTGGA CCATGGTGCT 
CCCATTCAAG CCAAAACAAA GAACGGTCTG TCACCGATCC ACATGGCAGC ACAGGGTGAC 
CACCTGGACT GTGTGCGCCT ACTCCTGCAG TACAGTGCTG AGATAGACGA CATCACCCTG 
GACCACCTGA CACCACTGCA TGTAGCTGCG CACTGTGGCC ACCACCGTGT GGCCAAGCTG 
CTGGTGGAGA AGGGAGCCAA GCCCAACTCC AGAGCTCTGA ATGGCTTCAC GCCTCTCCAC 
ATTGCCTGCA AGAAGAACCA CATTCGGGTG ATGGAGTTGC TTCTGAAGAC AGGAGCCTCC 
ATTGATGCTG TCACAGAGTC TGGCCTGACC CCCTTGCATG TGGCTGCCTT CATGGGGCAC 
TTGCCCATTG TCAAAACCTT GCTGCAGCGT GGAGCATCTC CTAATGTGTC CAATGTGAAA 
GTGGAGACTC CCCTGCACAT GGCAGCCAGA GCTGGGCACA TGGATGTGGC CAAGTATCTG 
ATACAGAACA AGGCCAAAAT CAACGCCAAG GCCAAGGATG ACCAGACTCC TCTGCACTGT 
GCTGCACGCA TTGGCCACAC CAGCATGGTC CAACTCCTGC TGGAGAACAA TGCCGACCCC 
AACCTGGCCA CTACAGCAGG GCACACGCCT CTGCACATCG CTGCCAGAGA GGGGCATGTA 
GACACAGCCT TGGCCCTGCT AGAGAAGGGA GCCTCTCAGA CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TCCACGTTGC AGCCAAGTAT GGGAAGGTGG ATGTGGCAGA GCTGCTGCTG 
GTGCATGATG CTCACCCCAA TGCAGCGGGG AAGAACGGCC TGACCCCACT GCACGTAGCT 
GTGCACCACA ACAACCTGGA GATCGTCAAG CTTCTGCTTC CCAAGGGGAG CTCTCCACAC 
AGCTCAGCCT GGAACGGGTA CACCCCTCTG CACATTGCCG CCAAGCAGAA CCAGATGGAG 
GTGGCCAGCA GCTTGCTGCA GTATGGTGCT TCTGCAAATG CGGAGTCTGT TCAGGGAGTC 
ACCCCTCTGC ACCTGGCTTC TCAGGAAGGG CATACAGACA TGGTAGCACT GCTTTTCTCC 
AAGCAAGCCA ATGGAAACTT AGGCAACAAG AGTGGTCTGA CTCCTCTCCA TCTCGTGGCC 
CAAGAGGGAC ACGTACCAGT GGCTGATGTT CTGGTGAAAC ATGGAGTCAC AGTGGATGCG 
ACAACCAGGA TGGGCTACAC CTCACTGCAC ATAGCCAGCC ACTATGGGAA CATCAAGTTG 
GTGAAGTTTC TGCTGCAGCA CCAGGCTGAT GTCAATGCTA AGACTAAGCT GGGCTACACA 
CCCCTGCACC AGGCGGCACA GCAGGGCCAC ACGGATGTGG TGACCCTGCT GCTGAAGCAT 
GGTGCCTCTC CCAACGAGAT CAGCACAAAT GGCACCACTC CCCTGGCCAT TGCGAAGCGC 
CTCGGTTACA TTTCCGTCAC AGATGTGCTC AAGATAGTCA CAGAGGAGAC CACCATCCCA 
CCATTCAGTG ACAAGTACCG CATGAGCTTC CCGGAGACTG TGGATGAGAT TCTGGATGTG 
TCGGAGGATG AAGGCACTGC TCATGTCACA GTAATGGAGG AGGAGCTGGT CCCACCGAAA 
CCCAGAACAC CTGAACCCAA GGAGCAGGAG GGCAAGAGGG AGATGCTGGA GTTTGTGACC 
GTGACAACAC TGGAGCAAAT GGAGGAATCT GCAGCTGTCC CACAGGTCCC CTGCGTGCCC 
CCTGAGACTG TGGTGACCAT AGCAGAGGAG TCTGAGCAGA TGGGACCTGT GGAGACTGAG 
GCTGAGCAAG GCGGCCTGCT GCCTGCACCC TCGGTGTCCC CACAGGAGCC CTCCAAGGAG 
TTCGATGAGG ACTCCTTGAT TCCCAGCAGC CCGGCCACCG AAACCTCGGA CAACATCAGC 
CCCGTGGCCA GCCCCGTTCA CACAGGGTTC CTGGTGAGCT TCATGGTGGA TGCGCGTGGC 
GGCTCCATGC GGGGCAGCCG GCACCACGGG CTGCGCGTGG TCATCCCTCC CCGTGCCTGT 
GCTGCACCCA CCCGCATCAC TTGCCGCCTG GTGAAGCCTC AGAAGCTGCC TGCCCCCCCA 
CCGCTGGCTG AGGAGGAGGG CCTGGCAAGC CGCATCATTG CACTGGGGCC CGCTGGCGCC 
CAGTTCCTCA GCCCCGTCAT TGTGGAGATT CCTCACTTTG CCTCCTATGG ACGTGGGGAC 
CGTGAGCTGG TGGTGTTGCG CAGTGAGAAT GGCTCTGTCT GGAAGGAGCA CCGCAACCGC 
TACGAGGAGA GCTACATGGA CCAACTGCTC AGTGGCATGG ATGAGGAGCT GGAGAGCCAA 
GAGGAGCTGG AGAAGAAGAG GGTCTGCCGC ATCATCACCA CTGACTTTCC TCTCTACTTC 
GTGGTCATGT CCCGGATTTG CCAGGACTGT GACATGATTG GCCCCGAGGG AGGGTGCTTG 
AAAAGCACAC TGGTGCCCAT GGTGCAGGCC ACCTTCCCAG ACACCGCTGT CACCAAGAAA 
GTGAGGCTGG CTCTGCAGGC GCAGCCCGTG CCTGATGAGC TGGTGACCAA GCTGCTGGGG 
AACCAGGCAA CCTTCAGCCC CATCGTCACA GTGGAGCCAC GTCGGAGGAA GTTCCACCGC 
CCCATTGGCC TCCGCATCCC TCTGCCCCCA TCCTGGAAGG ACAATCCCCG GGACAGCGGC 
GAGGGTGACA CCACCAGCCT GCGCCTGCTC TGTTCTGTGA TAGGAGGGAC AGCACAAGCT 
CAGTGGGAAG ACATCACAGG CACCACAAAG CTGGTCTATG AAAAAGAGTG TGCTAACTTT 
ACCACCAATG TGTCTGCCAG GTTCTGGCTG GCTGACTGCC CGCGCACTGC TGAGGCTGTG 
CACTTTGCCA CCGTGCTGTA CAAGGAACTG ACGGCTGTGC CCTACATGGC CAAATTTGTG 
GTGTTTGCTA AGATGAACGA TGGACGGGAA GGCCGGCTGC GCTGCTACTG CATGACTGAT 
GACAAGGTGG ACAAGACTTT GGAGCATCAT GAGAACTTCA CTGAGGTGGC CCGCAGCAGG 
GACATTGAGG TGGTGGAAGG AATGCCTCTG CATGTCGAGC TCTCAGGGAA CCTGGTGCCC 
GTCAAGAAGG CCACGCAGTC ACGCACCTTC TTCTTCCAGT CCTTCCGGGA AAGCCGCCTC 
GCCATCCCCA TCAAGGTCCG GGACAGCAGC CGGGAAGCCA GTGGCTCCCT GTCCTTCCTG 
CGCAAGGCCA TGAAGTATGA GGACTTGCAG CATGTGCTCT GCCACCTCAA CATCAGCATT 
CCACCCTGCA CCAAGGGAAG CAGCACTGAG GAGCGAAGGA GGACACTGAC ACCTTTGTCT 
CTGCGCTCTC TGAGCAGCAC CGACAGGGCA GACCAGAAGA TGATCGACAT TGCAGAGCAG 
CTTGGCCTCA GCTGGGCAGA GCTGGCACGT GAGCTGCAGT TTGGGGTGGA TGACATCAAC 
AGGATTCGTG TGGAGAACCC TAACTCCTTG CTGGAGCAGA GCATAGCCTT GCTTAACCTC 
TGGGTCAGCC GCGAGGGCAA GGGTGTCAAA ATTGAGAACC TGTACACAGC GCTGAGGAAC 
ATTGACCGCA GCGAGATCAT CAGCACGTTG GAGGGCTCCG GCCGGCAGAG CCGCAGCATG 
AAGGGCAGCT GGCGTTACAT GGACAGGGAC TATTCACTCT CGCCCTCCCA GATGAATGGT 
TACGCTTCGC TGCAGGACGA GCTGCTCTCC CCTGCCTCCC TGCATTATAT GCTGCCATCC 
CCTCTGCGTG CTGACCAGTA CTGGAACGAG GTGGCCATCA TGGACGCCAT CCCCATGGCC 
GCCACTGAGC AGGACGCCCT GATGGAGATG TCTGACATGC AGGTGTGGTC CTCAGGGCTC 
ACCCCTTCAC TGGTGACGGC TGAGGACTCC TCTCTGGAGT GCAGCAAGGC TGAGGACTCG 
GATGCTACAA GCGAAGGTCG TTTCCCAGGG CACCTTCTGA CAGATGCACA TGTCCCAGAC 
CACATGGGCT CTATGGACTT AGTTGAGGAT GACACAGTGG ACTCAGACGC CATGAACGGG 
CTGATTGAAC TGCTAGAGCA GGAGGAGGGG CAGAGGCCAG AGGGGAAGAT GCCAGCCAGC 
GACCACCAGC TAGGGACTGG AGAGCAGAAC CCAGAGAGTG AAGTCTCTTT TGTTTCAGTT 
CAACAGAAGG TACAAGCCAC GATCGTGCCA TCGCCAACCG TGAGCCACAC AGTGGAGAGG 
AGTGCAGACA GGCTGAGGGA CTGGAATGCA GAAGGCTCCT TTATCTCCTG CCTACAGGAC 
CTGACAGAGG GCTCCTGGCA GGAGGGGGTC ACCCGACGGC TGGGTCCAGC ACACACCGTG 
GCTGCTGGGG CGCAGGGCCA GGAGCAAGAG CAGGTCCTGG CGCCGGACGT GGAGCTGATG 
CGGGTCAGCT CCACAGAGGA CAGCGACTGG CAGCCCCAGC ACCCCGCGGG CGGCTGGCCA 
GAGGAGCCTG ACAGCCGCTG CTTTGGGCAG GGCAACGAAG TCCTCCATCT CCCTGGGGAG 
CATGTGACTG AGGAGCAGTT CACAGATGAA CAAGGCAATA TAATCACCAA GAAGGTCATC 
CGGAAAGTGG TGCATCAGTT GGACCTCGAC ACGGATGACA GGAAGGAGCA TGAGGACTTG 
ATTCTGGAGG GCTCCCTGCA GGAGCCCCAA GACCTGGAGG CAGAGGCTGA TCACTTCATG 
TACTCCATCC TACAGCGGGA CGGTCTGGGG GCCAAGGAGG AGGTGCAAGT GCGTGTCCCA 
AAACTGGAAG TCTCCGGGAG CAGGATGGGG GCTCAGATAG TGAAACGAGC CAGTCTGAAA 
AGGGGGAAGC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK1 ( XM_015297396.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152882.1 Gallus gallus ankyrin 1 (ANK1), transcript variant X7, mRNA.

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CloneID	OGa147284
Clone ID Related Accession (Same CDS sequence)	XM_015297396.2
Accession Version	XM_015297396.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ankyrin-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006109.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015297396.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATGCT TGCATTACTT CAGGGCTGAT GCTGCGACCA GCTTCCTGAG AGCTGCAAGA 
TCTGGGAATC TGGACAGAGC CTTGGATCAT CTCAGGAATG GGGTAGATAT TAACACCTGT 
AACCAGAATG GGCTGAATGC CTTGCACCTG GCCTCCAAGG AGGGCCACAC GAAAATGGTG 
GTGGAGCTTC TGCACAAGGA GATTGTTTTG GAGACCACTA CCAAGAAGGG AAACACAGCC 
CTGCACATCG CTGCCCTCGC TGGACAGCAG GACGTGGTCC GGGAACTGGT GAACTATGGG 
GCCAATGTCA ATGCTCAGTC GCAGAAGGGC TTCACACCAC TCTACATGGC AGCGCAGGAA 
AACCACCTGG AAGTTGTCAA ATTCCTGCTG GAAAACGGAG CCAACCAGAA TGTAGCCACA 
GAGGATGGCT TCACACCACT AGCTGTGGCT CTGCAGCAGG GTCATGAGAA TGTGGTTGCT 
CACCTTATCA ACTATGGGAC GAAGGGTAAG GTCCGCCTGC CTGCCTTGCA CATTGCAGCT 
CGCAACGATG ACACCCGTAC AGCCGCCGTG CTGCTGCAGA ATGACCCCAA TGCTGACGTC 
CTCTCCAAGA CTGGATTTAC ACCTTTGCAC ATTGCAGCCC ACTATGAGAA TCTCAGCGTG 
GCCCAATTAT TGCTGAATCG TGGAGCCAGC GTCAACTTCA CACCCCAGAA TGGGATCACT 
CCTTTGCACA TAGCCTCTCG TCGAGGCAAC ATCATCATGG TACGGCTGCT GCTGGACCGT 
GGGGCCCAGA TAGAGACAAG GACCAAGGAT GAGTTGACGC CTCTCCACTG TGCAGCTCGC 
AATGGACATG TGCGAATTGC AGAGATCCTA CTGGACCATG GTGCTCCCAT TCAAGCCAAA 
ACAAAGAACG GTCTGTCACC GATCCACATG GCAGCACAGG GTGACCACCT GGACTGTGTG 
CGCCTACTCC TGCAGTACAG TGCTGAGATA GACGACATCA CCCTGGACCA CCTGACACCA 
CTGCATGTAG CTGCGCACTG TGGCCACCAC CGTGTGGCCA AGCTGCTGGT GGAGAAGGGA 
GCCAAGCCCA ACTCCAGAGC TCTGAATGGC TTCACGCCTC TCCACATTGC CTGCAAGAAG 
AACCACATTC GGGTGATGGA GTTGCTTCTG AAGACAGGAG CCTCCATTGA TGCTGTCACA 
GAGTCTGGCC TGACCCCCTT GCATGTGGCT GCCTTCATGG GGCACTTGCC CATTGTCAAA 
ACCTTGCTGC AGCGTGGAGC ATCTCCTAAT GTGTCCAATG TGAAAGTGGA GACTCCCCTG 
CACATGGCAG CCAGAGCTGG GCACATGGAT GTGGCCAAGT ATCTGATACA GAACAAGGCC 
AAAATCAACG CCAAGGCCAA GGATGACCAG ACTCCTCTGC ACTGTGCTGC ACGCATTGGC 
CACACCAGCA TGGTCCAACT CCTGCTGGAG AACAATGCCG ACCCCAACCT GGCCACTACA 
GCAGGGCACA CGCCTCTGCA CATCGCTGCC AGAGAGGGGC ATGTAGACAC AGCCTTGGCC 
CTGCTAGAGA AGGGAGCCTC TCAGACCTGC ATGACCAAGA AAGGATTTAC CCCTCTCCAC 
GTTGCAGCCA AGTATGGGAA GGTGGATGTG GCAGAGCTGC TGCTGGTGCA TGATGCTCAC 
CCCAATGCAG CGGGGAAGAA CGGCCTGACC CCACTGCACG TAGCTGTGCA CCACAACAAC 
CTGGAGATCG TCAAGCTTCT GCTTCCCAAG GGGAGCTCTC CACACAGCTC AGCCTGGAAC 
GGGTACACCC CTCTGCACAT TGCCGCCAAG CAGAACCAGA TGGAGGTGGC CAGCAGCTTG 
CTGCAGTATG GTGCTTCTGC AAATGCGGAG TCTGTTCAGG GAGTCACCCC TCTGCACCTG 
GCTTCTCAGG AAGGGCATAC AGACATGGTA GCACTGCTTT TCTCCAAGCA AGCCAATGGA 
AACTTAGGCA ACAAGAGTGG TCTGACTCCT CTCCATCTCG TGGCCCAAGA GGGACACGTA 
CCAGTGGCTG ATGTTCTGGT GAAACATGGA GTCACAGTGG ATGCGACAAC CAGGATGGGC 
TACACCTCAC TGCACATAGC CAGCCACTAT GGGAACATCA AGTTGGTGAA GTTTCTGCTG 
CAGCACCAGG CTGATGTCAA TGCTAAGACT AAGCTGGGCT ACACACCCCT GCACCAGGCG 
GCACAGCAGG GCCACACGGA TGTGGTGACC CTGCTGCTGA AGCATGGTGC CTCTCCCAAC 
GAGATCAGCA CAAATGGCAC CACTCCCCTG GCCATTGCGA AGCGCCTCGG TTACATTTCC 
GTCACAGATG TGCTCAAGAT AGTCACAGAG GAGACCACCA TCCCACCATT CAGTGACAAG 
TACCGCATGA GCTTCCCGGA GACTGTGGAT GAGATTCTGG ATGTGTCGGA GGATGAAGGC 
ACTGCTCATG TCACAGTAAT GGAGGAGGAG CTGGTCCCAC CGAAACCCAG AACACCTGAA 
CCCAAGGAGC AGGAGGGCAA GAGGGAGATG CTGGAGTTTG TGACCGTGAC AACACTGGAG 
CAAATGGAGG AATCTGCAGC TGTCCCACAG GTCCCCTGCG TGCCCCCTGA GACTGTGGTG 
ACCATAGCAG AGGAGTCTGA GCAGATGGGA CCTGTGGAGA CTGAGGCTGA GCAAGGCGGC 
CTGCTGCCTG CACCCTCGGT GTCCCCACAG GAGCCCTCCA AGGAGTTCGA TGAGGACTCC 
TTGATTCCCA GCAGCCCGGC CACCGAAACC TCGGACAACA TCAGCCCCGT GGCCAGCCCC 
GTTCACACAG GGTTCCTGGT GAGCTTCATG GTGGATGCGC GTGGCGGCTC CATGCGGGGC 
AGCCGGCACC ACGGGCTGCG CGTGGTCATC CCTCCCCGTG CCTGTGCTGC ACCCACCCGC 
ATCACTTGCC GCCTGGTGAA GCCTCAGAAG CTGCCTGCCC CCCCACCGCT GGCTGAGGAG 
GAGGGCCTGG CAAGCCGCAT CATTGCACTG GGGCCCGCTG GCGCCCAGTT CCTCAGCCCC 
GTCATTGTGG AGATTCCTCA CTTTGCCTCC TATGGACGTG GGGACCGTGA GCTGGTGGTG 
TTGCGCAGTG AGAATGGCTC TGTCTGGAAG GAGCACCGCA ACCGCTACGA GGAGAGCTAC 
ATGGACCAAC TGCTCAGTGG CATGGATGAG GAGCTGGAGA GCCAAGAGGA GCTGGAGAAG 
AAGAGGGTCT GCCGCATCAT CACCACTGAC TTTCCTCTCT ACTTCGTGGT CATGTCCCGG 
ATTTGCCAGG ACTGTGACAT GATTGGCCCC GAGGGAGGGT GCTTGAAAAG CACACTGGTG 
CCCATGGTGC AGGCCACCTT CCCAGACACC GCTGTCACCA AGAAAGTGAG GCTGGCTCTG 
CAGGCGCAGC CCGTGCCTGA TGAGCTGGTG ACCAAGCTGC TGGGGAACCA GGCAACCTTC 
AGCCCCATCG TCACAGTGGA GCCACGTCGG AGGAAGTTCC ACCGCCCCAT TGGCCTCCGC 
ATCCCTCTGC CCCCATCCTG GAAGGACAAT CCCCGGGACA GCGGCGAGGG TGACACCACC 
AGCCTGCGCC TGCTCTGTTC TGTGATAGGA GGGACAGCAC AAGCTCAGTG GGAAGACATC 
ACAGGCACCA CAAAGCTGGT CTATGAAAAA GAGTGTGCTA ACTTTACCAC CAATGTGTCT 
GCCAGGTTCT GGCTGGCTGA CTGCCCGCGC ACTGCTGAGG CTGTGCACTT TGCCACCGTG 
CTGTACAAGG AACTGACGGC TGTGCCCTAC ATGGCCAAAT TTGTGGTGTT TGCTAAGATG 
AACGATGGAC GGGAAGGCCG GCTGCGCTGC TACTGCATGA CTGATGACAA GGTGGACAAG 
ACTTTGGAGC ATCATGAGAA CTTCACTGAG GTGGCCCGCA GCAGGGACAT TGAGGTGGTG 
GAAGGAATGC CTCTGCATGT CGAGCTCTCA GGGAACCTGG TGCCCGTCAA GAAGGCCACG 
CAGTCACGCA CCTTCTTCTT CCAGTCCTTC CGGGAAAGCC GCCTCGCCAT CCCCATCAAG 
GTCCGGGACA GCAGCCGGGA AGCCAGTGGC TCCCTGTCCT TCCTGCGCAA GGCCATGAAG 
TATGAGGACT TGCAGCATGT GCTCTGCCAC CTCAACATCA GCATTCCACC CTGCACCAAG 
GGAAGCAGCA CTGAGGAGCG AAGGAGGACA CTGACACCTT TGTCTCTGCG CTCTCTGAGC 
AGCACCGACA GGGCAGACCA GAAGATGATC GACATTGCAG AGCAGCTTGG CCTCAGCTGG 
GCAGAGCTGG CACGTGAGCT GCAGTTTGGG GTGGATGACA TCAACAGGAT TCGTGTGGAG 
AACCCTAACT CCTTGCTGGA GCAGAGCATA GCCTTGCTTA ACCTCTGGGT CAGCCGCGAG 
GGCAAGGGTG TCAAAATTGA GAACCTGTAC ACAGCGCTGA GGAACATTGA CCGCAGCGAG 
ATCATCAGCA CGTTGGAGGG CTCCGGCCGG CAGAGCCGCA GCATGAAGGG CAGCTGGCGT 
TACATGGACA GGGACTATTC ACTCTCGCCC TCCCAGATGA ATGGTTACGC TTCGCTGCAG 
GACGAGCTGC TCTCCCCTGC CTCCCTGCAT TATATGCTGC CATCCCCTCT GCGTGCTGAC 
CAGTACTGGA ACGAGGTGGC CATCATGGAC GCCATCCCCA TGGCCGCCAC TGAGCAGGAC 
GCCCTGATGG AGATGTCTGA CATGCAGGTG TGGTCCTCAG GGCTCACCCC TTCACTGGTG 
ACGGCTGAGG ACTCCTCTCT GGAGTGCAGC AAGGCTGAGG ACTCGGATGC TACAAGCGAA 
GGTCGTTTCC CAGGGCACCT TCTGACAGAT GCACATGTCC CAGACCACAT GGGCTCTATG 
GACTTAGTTG AGGATGACAC AGTGGACTCA GACGCCATGA ACGGGCTGAT TGAACTGCTA 
GAGCAGGAGG AGGGGCAGAG GCCAGAGGGG AAGATGCCAG CCAGCGACCA CCAGCTAGGG 
ACTGGAGAGC AGAACCCAGA GAGTGAAGTC TCTTTTGTTT CAGTTCAACA GAAGGTACAA 
GCCACGATCG TGCCATCGCC AACCGTGAGC CACACAGTGG AGAGGAGTGC AGACAGGCTG 
AGGGACTGGA ATGCAGAAGG CTCCTTTATC TCCTGCCTAC AGGACCTGAC AGAGGGCTCC 
TGGCAGGAGG GGGTCACCCG ACGGCTGGGT CCAGCACACA CCGTGGCTGC TGGGGCGCAG 
GGCCAGGAGC AAGAGCAGGT CCTGGCGCCG GACGTGGAGC TGATGCGGGT CAGCTCCACA 
GAGGACAGCG ACTGGCAGCC CCAGCACCCC GCGGGCGGCT GGCCAGAGGA GCCTGACAGC 
CGCTGCTTTG GGCAGGGCAA CGAAGTCCTC CATCTCCCTG GGGAGCATGT GACTGAGGAG 
CAGTTCACAG ATGAACAAGG CAATATAATC ACCAAGAAGG TCATCCGGAA AGTGGTGCAT 
CAGTTGGACC TCGACACGGA TGACAGGAAG GAGCATGAGG ACTTGATTCT GGAGGGCTCC 
CTGCAGGAGC CCCAAGACCT GGAGGCAGAG GCTGATCACT TCATGTACTC CATCCTACAG 
CGGGACGGTC TGGGGGCCAA GGAGGAGGTG CAAGTGCGTG TCCCAAAACT GGAAGTCTCC 
GGGAGCAGGA TGGGGGCTCA GATAGTGAAA CGAGCCAGTC TGAAAAGGGG GAAGCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152882.1
CDS	109..5868
Translation	MPCLHYFRADAATSFLRAARSGNLDRALDHLRNGVDINTCNQNGLNALHLASKEGHTKMVVELLHKEIVLETTTKKGNTALHIAALAGQQDVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLSVAQLLLNRGASVNFTPQNGITPLHIASRRGNIIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRIAEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYSAEIDDITLDHLTPLHVAAHCGHHRVAKLLVEKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVAAFMGHLPIVKTLLQRGASPNVSNVKVETPLHMAARAGHMDVAKYLIQNKAKINAKAKDDQTPLHCAARIGHTSMVQLLLENNADPNLATTAGHTPLHIAAREGHVDTALALLEKGASQTCMTKKGFTPLHVAAKYGKVDVAELLLVHDAHPNAAGKNGLTPLHVAVHHNNLEIVKLLLPKGSSPHSSAWNGYTPLHIAAKQNQMEVASSLLQYGASANAESVQGVTPLHLASQEGHTDMVALLFSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLVKHGVTVDATTRMGYTSLHIASHYGNIKLVKFLLQHQADVNAKTKLGYTPLHQAAQQGHTDVVTLLLKHGASPNEISTNGTTPLAIAKRLGYISVTDVLKIVTEETTIPPFSDKYRMSFPETVDEILDVSEDEGTAHVTVMEEELVPPKPRTPEPKEQEGKREMLEFVTVTTLEQMEESAAVPQVPCVPPETVVTIAEESEQMGPVETEAEQGGLLPAPSVSPQEPSKEFDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHHGLRVVIPPRACAAPTRITCRLVKPQKLPAPPPLAEEEGLASRIIALGPAGAQFLSPVIVEIPHFASYGRGDRELVVLRSENGSVWKEHRNRYEESYMDQLLSGMDEELESQEELEKKRVCRIITTDFPLYFVVMSRICQDCDMIGPEGGCLKSTLVPMVQATFPDTAVTKKVRLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWKDNPRDSGEGDTTSLRLLCSVIGGTAQAQWEDITGTTKLVYEKECANFTTNVSARFWLADCPRTAEAVHFATVLYKELTAVPYMAKFVVFAKMNDGREGRLRCYCMTDDKVDKTLEHHENFTEVARSRDIEVVEGMPLHVELSGNLVPVKKATQSRTFFFQSFRESRLAIPIKVRDSSREASGSLSFLRKAMKYEDLQHVLCHLNISIPPCTKGSSTEERRRTLTPLSLRSLSSTDRADQKMIDIAEQLGLSWAELARELQFGVDDINRIRVENPNSLLEQSIALLNLWVSREGKGVKIENLYTALRNIDRSEIISTLEGSGRQSRSMKGSWRYMDRDYSLSPSQMNGYASLQDELLSPASLHYMLPSPLRADQYWNEVAIMDAIPMAATEQDALMEMSDMQVWSSGLTPSLVTAEDSSLECSKAEDSDATSEGRFPGHLLTDAHVPDHMGSMDLVEDDTVDSDAMNGLIELLEQEEGQRPEGKMPASDHQLGTGEQNPESEVSFVSVQQKVQATIVPSPTVSHTVERSADRLRDWNAEGSFISCLQDLTEGSWQEGVTRRLGPAHTVAAGAQGQEQEQVLAPDVELMRVSSTEDSDWQPQHPAGGWPEEPDSRCFGQGNEVLHLPGEHVTEEQFTDEQGNIITKKVIRKVVHQLDLDTDDRKEHEDLILEGSLQEPQDLEAEADHFMYSILQRDGLGAKEEVQVRVPKLEVSGSRMGAQIVKRASLKRGKH

Target ORF information:

RefSeq Version	XM_015297396.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ankyrin 1 (ANK1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297396.2

ORF Insert Sequence:

ATGCCATGCT TGCATTACTT CAGGGCTGAT GCTGCGACCA GCTTCCTGAG AGCTGCAAGA 
TCTGGGAATC TGGACAGAGC CTTGGATCAT CTCAGGAATG GGGTAGATAT TAACACCTGT 
AACCAGAATG GGCTGAATGC CTTGCACCTG GCCTCCAAGG AGGGCCACAC GAAAATGGTG 
GTGGAGCTTC TGCACAAGGA GATTGTTTTG GAGACCACTA CCAAGAAGGG AAACACAGCC 
CTGCACATCG CTGCCCTCGC TGGACAGCAG GACGTGGTCC GGGAACTGGT GAACTATGGG 
GCCAATGTCA ATGCTCAGTC GCAGAAGGGC TTCACACCAC TCTACATGGC AGCGCAGGAA 
AACCACCTGG AAGTTGTCAA ATTCCTGCTG GAAAACGGAG CCAACCAGAA TGTAGCCACA 
GAGGATGGCT TCACACCACT AGCTGTGGCT CTGCAGCAGG GTCATGAGAA TGTGGTTGCT 
CACCTTATCA ACTATGGGAC GAAGGGTAAG GTCCGCCTGC CTGCCTTGCA CATTGCAGCT 
CGCAACGATG ACACCCGTAC AGCCGCCGTG CTGCTGCAGA ATGACCCCAA TGCTGACGTC 
CTCTCCAAGA CTGGATTTAC ACCTTTGCAC ATTGCAGCCC ACTATGAGAA TCTCAGCGTG 
GCCCAATTAT TGCTGAATCG TGGAGCCAGC GTCAACTTCA CACCCCAGAA TGGGATCACT 
CCTTTGCACA TAGCCTCTCG TCGAGGCAAC ATCATCATGG TACGGCTGCT GCTGGACCGT 
GGGGCCCAGA TAGAGACAAG GACCAAGGAT GAGTTGACGC CTCTCCACTG TGCAGCTCGC 
AATGGACATG TGCGAATTGC AGAGATCCTA CTGGACCATG GTGCTCCCAT TCAAGCCAAA 
ACAAAGAACG GTCTGTCACC GATCCACATG GCAGCACAGG GTGACCACCT GGACTGTGTG 
CGCCTACTCC TGCAGTACAG TGCTGAGATA GACGACATCA CCCTGGACCA CCTGACACCA 
CTGCATGTAG CTGCGCACTG TGGCCACCAC CGTGTGGCCA AGCTGCTGGT GGAGAAGGGA 
GCCAAGCCCA ACTCCAGAGC TCTGAATGGC TTCACGCCTC TCCACATTGC CTGCAAGAAG 
AACCACATTC GGGTGATGGA GTTGCTTCTG AAGACAGGAG CCTCCATTGA TGCTGTCACA 
GAGTCTGGCC TGACCCCCTT GCATGTGGCT GCCTTCATGG GGCACTTGCC CATTGTCAAA 
ACCTTGCTGC AGCGTGGAGC ATCTCCTAAT GTGTCCAATG TGAAAGTGGA GACTCCCCTG 
CACATGGCAG CCAGAGCTGG GCACATGGAT GTGGCCAAGT ATCTGATACA GAACAAGGCC 
AAAATCAACG CCAAGGCCAA GGATGACCAG ACTCCTCTGC ACTGTGCTGC ACGCATTGGC 
CACACCAGCA TGGTCCAACT CCTGCTGGAG AACAATGCCG ACCCCAACCT GGCCACTACA 
GCAGGGCACA CGCCTCTGCA CATCGCTGCC AGAGAGGGGC ATGTAGACAC AGCCTTGGCC 
CTGCTAGAGA AGGGAGCCTC TCAGACCTGC ATGACCAAGA AAGGATTTAC CCCTCTCCAC 
GTTGCAGCCA AGTATGGGAA GGTGGATGTG GCAGAGCTGC TGCTGGTGCA TGATGCTCAC 
CCCAATGCAG CGGGGAAGAA CGGCCTGACC CCACTGCACG TAGCTGTGCA CCACAACAAC 
CTGGAGATCG TCAAGCTTCT GCTTCCCAAG GGGAGCTCTC CACACAGCTC AGCCTGGAAC 
GGGTACACCC CTCTGCACAT TGCCGCCAAG CAGAACCAGA TGGAGGTGGC CAGCAGCTTG 
CTGCAGTATG GTGCTTCTGC AAATGCGGAG TCTGTTCAGG GAGTCACCCC TCTGCACCTG 
GCTTCTCAGG AAGGGCATAC AGACATGGTA GCACTGCTTT TCTCCAAGCA AGCCAATGGA 
AACTTAGGCA ACAAGAGTGG TCTGACTCCT CTCCATCTCG TGGCCCAAGA GGGACACGTA 
CCAGTGGCTG ATGTTCTGGT GAAACATGGA GTCACAGTGG ATGCGACAAC CAGGATGGGC 
TACACCTCAC TGCACATAGC CAGCCACTAT GGGAACATCA AGTTGGTGAA GTTTCTGCTG 
CAGCACCAGG CTGATGTCAA TGCTAAGACT AAGCTGGGCT ACACACCCCT GCACCAGGCG 
GCACAGCAGG GCCACACGGA TGTGGTGACC CTGCTGCTGA AGCATGGTGC CTCTCCCAAC 
GAGATCAGCA CAAATGGCAC CACTCCCCTG GCCATTGCGA AGCGCCTCGG TTACATTTCC 
GTCACAGATG TGCTCAAGAT AGTCACAGAG GAGACCACCA TCCCACCATT CAGTGACAAG 
TACCGCATGA GCTTCCCGGA GACTGTGGAT GAGATTCTGG ATGTGTCGGA GGATGAAGGC 
ACTGCTCATG TCACAGTAAT GGAGGAGGAG CTGGTCCCAC CGAAACCCAG AACACCTGAA 
CCCAAGGAGC AGGAGGGCAA GAGGGAGATG CTGGAGTTTG TGACCGTGAC AACACTGGAG 
CAAATGGAGG AATCTGCAGC TGTCCCACAG GTCCCCTGCG TGCCCCCTGA GACTGTGGTG 
ACCATAGCAG AGGAGTCTGA GCAGATGGGA CCTGTGGAGA CTGAGGCTGA GCAAGGCGGC 
CTGCTGCCTG CACCCTCGGT GTCCCCACAG GAGCCCTCCA AGGAGTTCGA TGAGGACTCC 
TTGATTCCCA GCAGCCCGGC CACCGAAACC TCGGACAACA TCAGCCCCGT GGCCAGCCCC 
GTTCACACAG GGTTCCTGGT GAGCTTCATG GTGGATGCGC GTGGCGGCTC CATGCGGGGC 
AGCCGGCACC ACGGGCTGCG CGTGGTCATC CCTCCCCGTG CCTGTGCTGC ACCCACCCGC 
ATCACTTGCC GCCTGGTGAA GCCTCAGAAG CTGCCTGCCC CCCCACCGCT GGCTGAGGAG 
GAGGGCCTGG CAAGCCGCAT CATTGCACTG GGGCCCGCTG GCGCCCAGTT CCTCAGCCCC 
GTCATTGTGG AGATTCCTCA CTTTGCCTCC TATGGACGTG GGGACCGTGA GCTGGTGGTG 
TTGCGCAGTG AGAATGGCTC TGTCTGGAAG GAGCACCGCA ACCGCTACGA GGAGAGCTAC 
ATGGACCAAC TGCTCAGTGG CATGGATGAG GAGCTGGAGA GCCAAGAGGA GCTGGAGAAG 
AAGAGGGTCT GCCGCATCAT CACCACTGAC TTTCCTCTCT ACTTCGTGGT CATGTCCCGG 
ATTTGCCAGG ACTGTGACAT GATTGGCCCC GAGGGAGGGT GCTTGAAAAG CACACTGGTG 
CCCATGGTGC AGGCCACCTT CCCAGACACC GCTGTCACCA AGAAAGTGAG GCTGGCTCTG 
CAGGCGCAGC CCGTGCCTGA TGAGCTGGTG ACCAAGCTGC TGGGGAACCA GGCAACCTTC 
AGCCCCATCG TCACAGTGGA GCCACGTCGG AGGAAGTTCC ACCGCCCCAT TGGCCTCCGC 
ATCCCTCTGC CCCCATCCTG GAAGGACAAT CCCCGGGACA GCGGCGAGGG TGACACCACC 
AGCCTGCGCC TGCTCTGTTC TGTGATAGGA GGGACAGCAC AAGCTCAGTG GGAAGACATC 
ACAGGCACCA CAAAGCTGGT CTATGAAAAA GAGTGTGCTA ACTTTACCAC CAATGTGTCT 
GCCAGGTTCT GGCTGGCTGA CTGCCCGCGC ACTGCTGAGG CTGTGCACTT TGCCACCGTG 
CTGTACAAGG AACTGACGGC TGTGCCCTAC ATGGCCAAAT TTGTGGTGTT TGCTAAGATG 
AACGATGGAC GGGAAGGCCG GCTGCGCTGC TACTGCATGA CTGATGACAA GGTGGACAAG 
ACTTTGGAGC ATCATGAGAA CTTCACTGAG GTGGCCCGCA GCAGGGACAT TGAGGTGGTG 
GAAGGAATGC CTCTGCATGT CGAGCTCTCA GGGAACCTGG TGCCCGTCAA GAAGGCCACG 
CAGTCACGCA CCTTCTTCTT CCAGTCCTTC CGGGAAAGCC GCCTCGCCAT CCCCATCAAG 
GTCCGGGACA GCAGCCGGGA AGCCAGTGGC TCCCTGTCCT TCCTGCGCAA GGCCATGAAG 
TATGAGGACT TGCAGCATGT GCTCTGCCAC CTCAACATCA GCATTCCACC CTGCACCAAG 
GGAAGCAGCA CTGAGGAGCG AAGGAGGACA CTGACACCTT TGTCTCTGCG CTCTCTGAGC 
AGCACCGACA GGGCAGACCA GAAGATGATC GACATTGCAG AGCAGCTTGG CCTCAGCTGG 
GCAGAGCTGG CACGTGAGCT GCAGTTTGGG GTGGATGACA TCAACAGGAT TCGTGTGGAG 
AACCCTAACT CCTTGCTGGA GCAGAGCATA GCCTTGCTTA ACCTCTGGGT CAGCCGCGAG 
GGCAAGGGTG TCAAAATTGA GAACCTGTAC ACAGCGCTGA GGAACATTGA CCGCAGCGAG 
ATCATCAGCA CGTTGGAGGG CTCCGGCCGG CAGAGCCGCA GCATGAAGGG CAGCTGGCGT 
TACATGGACA GGGACTATTC ACTCTCGCCC TCCCAGATGA ATGGTTACGC TTCGCTGCAG 
GACGAGCTGC TCTCCCCTGC CTCCCTGCAT TATATGCTGC CATCCCCTCT GCGTGCTGAC 
CAGTACTGGA ACGAGGTGGC CATCATGGAC GCCATCCCCA TGGCCGCCAC TGAGCAGGAC 
GCCCTGATGG AGATGTCTGA CATGCAGGTG TGGTCCTCAG GGCTCACCCC TTCACTGGTG 
ACGGCTGAGG ACTCCTCTCT GGAGTGCAGC AAGGCTGAGG ACTCGGATGC TACAAGCGAA 
GGTCGTTTCC CAGGGCACCT TCTGACAGAT GCACATGTCC CAGACCACAT GGGCTCTATG 
GACTTAGTTG AGGATGACAC AGTGGACTCA GACGCCATGA ACGGGCTGAT TGAACTGCTA 
GAGCAGGAGG AGGGGCAGAG GCCAGAGGGG AAGATGCCAG CCAGCGACCA CCAGCTAGGG 
ACTGGAGAGC AGAACCCAGA GAGTGAAGTC TCTTTTGTTT CAGTTCAACA GAAGGTACAA 
GCCACGATCG TGCCATCGCC AACCGTGAGC CACACAGTGG AGAGGAGTGC AGACAGGCTG 
AGGGACTGGA ATGCAGAAGG CTCCTTTATC TCCTGCCTAC AGGACCTGAC AGAGGGCTCC 
TGGCAGGAGG GGGTCACCCG ACGGCTGGGT CCAGCACACA CCGTGGCTGC TGGGGCGCAG 
GGCCAGGAGC AAGAGCAGGT CCTGGCGCCG GACGTGGAGC TGATGCGGGT CAGCTCCACA 
GAGGACAGCG ACTGGCAGCC CCAGCACCCC GCGGGCGGCT GGCCAGAGGA GCCTGACAGC 
CGCTGCTTTG GGCAGGGCAA CGAAGTCCTC CATCTCCCTG GGGAGCATGT GACTGAGGAG 
CAGTTCACAG ATGAACAAGG CAATATAATC ACCAAGAAGG TCATCCGGAA AGTGGTGCAT 
CAGTTGGACC TCGACACGGA TGACAGGAAG GAGCATGAGG ACTTGATTCT GGAGGGCTCC 
CTGCAGGAGC CCCAAGACCT GGAGGCAGAG GCTGATCACT TCATGTACTC CATCCTACAG 
CGGGACGGTC TGGGGGCCAA GGAGGAGGTG CAAGTGCGTG TCCCAAAACT GGAAGTCTCC 
GGGAGCAGGA TGGGGGCTCA GATAGTGAAA CGAGCCAGTC TGAAAAGGGG GAAGCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK1 ( XM_025142840.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024998608.1 Gallus gallus ankyrin 1 (ANK1), transcript variant X8, mRNA.

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CloneID	OGa147285
Clone ID Related Accession (Same CDS sequence)	XM_025142840.1
Accession Version	XM_025142840.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ankyrin-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006109.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTGG AGCTTCTGCA CAAGGAGATT GTTTTGGAGA CCACTACCAA GAAGGGAAAC 
ACAGCCCTGC ACATCGCTGC CCTCGCTGGA CAGCAGGACG TGGTCCGGGA ACTGGTGAAC 
TATGGGGCCA ATGTCAATGC TCAGTCGCAG AAGGGCTTCA CACCACTCTA CATGGCAGCG 
CAGGAAAACC ACCTGGAAGT TGTCAAATTC CTGCTGGAAA ACGGAGCCAA CCAGAATGTA 
GCCACAGAGG ATGGCTTCAC ACCACTAGCT GTGGCTCTGC AGCAGGGTCA TGAGAATGTG 
GTTGCTCACC TTATCAACTA TGGGACGAAG GGTAAGGTCC GCCTGCCTGC CTTGCACATT 
GCAGCTCGCA ACGATGACAC CCGTACAGCC GCCGTGCTGC TGCAGAATGA CCCCAATGCT 
GACGTCCTCT CCAAGACTGG ATTTACACCT TTGCACATTG CAGCCCACTA TGAGAATCTC 
AGCGTGGCCC AATTATTGCT GAATCGTGGA GCCAGCGTCA ACTTCACACC CCAGAATGGG 
ATCACTCCTT TGCACATAGC CTCTCGTCGA GGCAACATCA TCATGGTACG GCTGCTGCTG 
GACCGTGGGG CCCAGATAGA GACAAGGACC AAGGATGAGT TGACGCCTCT CCACTGTGCA 
GCTCGCAATG GACATGTGCG AATTGCAGAG ATCCTACTGG ACCATGGTGC TCCCATTCAA 
GCCAAAACAA AGAACGGTCT GTCACCGATC CACATGGCAG CACAGGGTGA CCACCTGGAC 
TGTGTGCGCC TACTCCTGCA GTACAGTGCT GAGATAGACG ACATCACCCT GGACCACCTG 
ACACCACTGC ATGTAGCTGC GCACTGTGGC CACCACCGTG TGGCCAAGCT GCTGGTGGAG 
AAGGGAGCCA AGCCCAACTC CAGAGCTCTG AATGGCTTCA CGCCTCTCCA CATTGCCTGC 
AAGAAGAACC ACATTCGGGT GATGGAGTTG CTTCTGAAGA CAGGAGCCTC CATTGATGCT 
GTCACAGAGT CTGGCCTGAC CCCCTTGCAT GTGGCTGCCT TCATGGGGCA CTTGCCCATT 
GTCAAAACCT TGCTGCAGCG TGGAGCATCT CCTAATGTGT CCAATGTGAA AGTGGAGACT 
CCCCTGCACA TGGCAGCCAG AGCTGGGCAC ATGGATGTGG CCAAGTATCT GATACAGAAC 
AAGGCCAAAA TCAACGCCAA GGCCAAGGAT GACCAGACTC CTCTGCACTG TGCTGCACGC 
ATTGGCCACA CCAGCATGGT CCAACTCCTG CTGGAGAACA ATGCCGACCC CAACCTGGCC 
ACTACAGCAG GGCACACGCC TCTGCACATC GCTGCCAGAG AGGGGCATGT AGACACAGCC 
TTGGCCCTGC TAGAGAAGGG AGCCTCTCAG ACCTGCATGA CCAAGAAAGG ATTTACCCCT 
CTCCACGTTG CAGCCAAGTA TGGGAAGGTG GATGTGGCAG AGCTGCTGCT GGTGCATGAT 
GCTCACCCCA ATGCAGCGGG GAAGAACGGC CTGACCCCAC TGCACGTAGC TGTGCACCAC 
AACAACCTGG AGATCGTCAA GCTTCTGCTT CCCAAGGGGA GCTCTCCACA CAGCTCAGCC 
TGGAACGGGT ACACCCCTCT GCACATTGCC GCCAAGCAGA ACCAGATGGA GGTGGCCAGC 
AGCTTGCTGC AGTATGGTGC TTCTGCAAAT GCGGAGTCTG TTCAGGGAGT CACCCCTCTG 
CACCTGGCTT CTCAGGAAGG GCATACAGAC ATGGTAGCAC TGCTTTTCTC CAAGCAAGCC 
AATGGAAACT TAGGCAACAA GAGTGGTCTG ACTCCTCTCC ATCTCGTGGC CCAAGAGGGA 
CACGTACCAG TGGCTGATGT TCTGGTGAAA CATGGAGTCA CAGTGGATGC GACAACCAGG 
ATGGGCTACA CCTCACTGCA CATAGCCAGC CACTATGGGA ACATCAAGTT GGTGAAGTTT 
CTGCTGCAGC ACCAGGCTGA TGTCAATGCT AAGACTAAGC TGGGCTACAC ACCCCTGCAC 
CAGGCGGCAC AGCAGGGCCA CACGGATGTG GTGACCCTGC TGCTGAAGCA TGGTGCCTCT 
CCCAACGAGA TCAGCACAAA TGGCACCACT CCCCTGGCCA TTGCGAAGCG CCTCGGTTAC 
ATTTCCGTCA CAGATGTGCT CAAGATAGTC ACAGAGGAGA CCACCATCCC ACCATTCAGT 
GACAAGTACC GCATGAGCTT CCCGGAGACT GTGGATGAGA TTCTGGATGT GTCGGAGGAT 
GAAGGCACTG CTCATGTCAC AGTAATGGAG GAGGAGCTGG TCCCACCGAA ACCCAGAACA 
CCTGAACCCA AGGAGCAGGA GGGCAAGAGG GAGATGCTGG AGTTTGTGAC CGTGACAACA 
CTGGAGCAAA TGGAGGAATC TGCAGCTGTC CCACAGGTCC CCTGCGTGCC CCCTGAGACT 
GTGGTGACCA TAGCAGAGGA GTCTGAGCAG ATGGGACCTG TGGAGACTGA GGCTGAGCAA 
GGCGGCCTGC TGCCTGCACC CTCGGTGTCC CCACAGGAGC CCTCCAAGGA GTTCGATGAG 
GACTCCTTGA TTCCCAGCAG CCCGGCCACC GAAACCTCGG ACAACATCAG CCCCGTGGCC 
AGCCCCGTTC ACACAGGGTT CCTGGTGAGC TTCATGGTGG ATGCGCGTGG CGGCTCCATG 
CGGGGCAGCC GGCACCACGG GCTGCGCGTG GTCATCCCTC CCCGTGCCTG TGCTGCACCC 
ACCCGCATCA CTTGCCGCCT GGTGAAGCCT CAGAAGCTGC CTGCCCCCCC ACCGCTGGCT 
GAGGAGGAGG GCCTGGCAAG CCGCATCATT GCACTGGGGC CCGCTGGCGC CCAGTTCCTC 
AGCCCCGTCA TTGTGGAGAT TCCTCACTTT GCCTCCTATG GACGTGGGGA CCGTGAGCTG 
GTGGTGTTGC GCAGTGAGAA TGGCTCTGTC TGGAAGGAGC ACCGCAACCG CTACGAGGAG 
AGCTACATGG ACCAACTGCT CAGTGGCATG GATGAGGAGC TGGAGAGCCA AGAGGAGCTG 
GAGAAGAAGA GGGTCTGCCG CATCATCACC ACTGACTTTC CTCTCTACTT CGTGGTCATG 
TCCCGGATTT GCCAGGACTG TGACATGATT GGCCCCGAGG GAGGGTGCTT GAAAAGCACA 
CTGGTGCCCA TGGTGCAGGC CACCTTCCCA GACACCGCTG TCACCAAGAA AGTGAGGCTG 
GCTCTGCAGG CGCAGCCCGT GCCTGATGAG CTGGTGACCA AGCTGCTGGG GAACCAGGCA 
ACCTTCAGCC CCATCGTCAC AGTGGAGCCA CGTCGGAGGA AGTTCCACCG CCCCATTGGC 
CTCCGCATCC CTCTGCCCCC ATCCTGGAAG GACAATCCCC GGGACAGCGG CGAGGGTGAC 
ACCACCAGCC TGCGCCTGCT CTGTTCTGTG ATAGGAGGGA CAGCACAAGC TCAGTGGGAA 
GACATCACAG GCACCACAAA GCTGGTCTAT GAAAAAGAGT GTGCTAACTT TACCACCAAT 
GTGTCTGCCA GGTTCTGGCT GGCTGACTGC CCGCGCACTG CTGAGGCTGT GCACTTTGCC 
ACCGTGCTGT ACAAGGAACT GACGGCTGTG CCCTACATGG CCAAATTTGT GGTGTTTGCT 
AAGATGAACG ATGGACGGGA AGGCCGGCTG CGCTGCTACT GCATGACTGA TGACAAGGTG 
GACAAGACTT TGGAGCATCA TGAGAACTTC ACTGAGGTGG CCCGCAGCAG GGACATTGAG 
GTGGTGGAAG GAATGCCTCT GCATGTCGAG CTCTCAGGGA ACCTGGTGCC CGTCAAGAAG 
GCCACGCAGT CACGCACCTT CTTCTTCCAG TCCTTCCGGG AAAGCCGCCT CGCCATCCCC 
ATCAAGGTCC GGGACAGCAG CCGGGAAGCC AGTGGCTCCC TGTCCTTCCT GCGCAAGGCC 
ATGAAGTATG AGGACTTGCA GCATGTGCTC TGCCACCTCA ACATCAGCAT TCCACCCTGC 
ACCAAGGGAA GCAGCACTGA GGAGCGAAGG AGGACACTGA CACCTTTGTC TCTGCGCTCT 
CTGAGCAGCA CCGACAGGGC AGACCAGAAG ATGATCGACA TTGCAGAGCA GCTTGGCCTC 
AGCTGGGCAG AGCTGGCACG TGAGCTGCAG TTTGGGGTGG ATGACATCAA CAGGATTCGT 
GTGGAGAACC CTAACTCCTT GCTGGAGCAG AGCATAGCCT TGCTTAACCT CTGGGTCAGC 
CGCGAGGGCA AGGGTGTCAA AATTGAGAAC CTGTACACAG CGCTGAGGAA CATTGACCGC 
AGCGAGATCA TCAGCACGTT GGAGGGCTCC GGCCGGCAGA GCCGCAGCAT GAAGGGCAGC 
TGGCGTTACA TGGACAGGGA CTATTCACTC TCGCCCTCCC AGATGAATGG TTACGCTTCG 
CTGCAGGACG AGCTGCTCTC CCCTGCCTCC CTGCATTATA TGCTGCCATC CCCTCTGCGT 
GCTGACCAGT ACTGGAACGA GGTGGCCATC ATGGACGCCA TCCCCATGGC CGCCACTGAG 
CAGGACGCCC TGATGGAGAT GTCTGACATG CAGGTGTGGT CCTCAGGGCT CACCCCTTCA 
CTGGTGACGG CTGAGGACTC CTCTCTGGAG TGCAGCAAGG CTGAGGACTC GGATGCTACA 
AGCGAAGGTC GTTTCCCAGG GCACCTTCTG ACAGATGCAC ATGTCCCAGA CCACATGGGC 
TCTATGGACT TAGTTGAGGA TGACACAGTG GACTCAGACG CCATGAACGG GCTGATTGAA 
CTGCTAGAGC AGGAGGAGGG GCAGAGGCCA GAGGGGAAGA TGCCAGCCAG CGACCACCAG 
CTAGGGACTG GAGAGCAGAA CCCAGAGAGT GAAGTCTCTT TTGTTTCAGT TCAACAGAAG 
GTACAAGCCA CGATCGTGCC ATCGCCAACC GTGAGCCACA CAGTGGAGAG GAGTGCAGAC 
AGGCTGAGGG ACTGGAATGC AGAAGGCTCC TTTATCTCCT GCCTACAGGA CCTGACAGAG 
GGCTCCTGGC AGGAGGGGGT CACCCGACGG CTGGGTCCAG CACACACCGT GGCTGCTGGG 
GCGCAGGGCC AGGAGCAAGA GCAGGTCCTG GCGCCGGACG TGGAGCTGAT GCGGGTCAGC 
TCCACAGAGG ACAGCGACTG GCAGCCCCAG CACCCCGCGG GCGGCTGGCC AGAGGAGCCT 
GACAGCCGCT GCTTTGGGCA GGGCAACGAA GTCCTCCATC TCCCTGGGGA GCATGTGACT 
GAGGAGCAGT TCACAGATGA ACAAGGCAAT ATAATCACCA AGAAGGTCAT CCGGAAAGTG 
GTGCATCAGT TGGACCTCGA CACGGATGAC AGGAAGGAGC ATGAGGACTT GATTCTGGAG 
GGCTCCCTGC AGGAGCCCCA AGACCTGGAG GCAGAGGCTG ATCACTTCAT GTACTCCATC 
CTACAGCGGG ACGGTCTGGG GGCCAAGGAG GAGGTGCAAG TGCGTGTCCC AAAACTGGAA 
GTCTCCGGGA GCAGGATGGG GGCTCAGATA GTGAAACGAG CCAGTCTGAA AAGGGGGAAG 
CATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024998608.1
CDS	170..5755
Translation	MVVELLHKEIVLETTTKKGNTALHIAALAGQQDVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLSVAQLLLNRGASVNFTPQNGITPLHIASRRGNIIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRIAEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYSAEIDDITLDHLTPLHVAAHCGHHRVAKLLVEKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVAAFMGHLPIVKTLLQRGASPNVSNVKVETPLHMAARAGHMDVAKYLIQNKAKINAKAKDDQTPLHCAARIGHTSMVQLLLENNADPNLATTAGHTPLHIAAREGHVDTALALLEKGASQTCMTKKGFTPLHVAAKYGKVDVAELLLVHDAHPNAAGKNGLTPLHVAVHHNNLEIVKLLLPKGSSPHSSAWNGYTPLHIAAKQNQMEVASSLLQYGASANAESVQGVTPLHLASQEGHTDMVALLFSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLVKHGVTVDATTRMGYTSLHIASHYGNIKLVKFLLQHQADVNAKTKLGYTPLHQAAQQGHTDVVTLLLKHGASPNEISTNGTTPLAIAKRLGYISVTDVLKIVTEETTIPPFSDKYRMSFPETVDEILDVSEDEGTAHVTVMEEELVPPKPRTPEPKEQEGKREMLEFVTVTTLEQMEESAAVPQVPCVPPETVVTIAEESEQMGPVETEAEQGGLLPAPSVSPQEPSKEFDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHHGLRVVIPPRACAAPTRITCRLVKPQKLPAPPPLAEEEGLASRIIALGPAGAQFLSPVIVEIPHFASYGRGDRELVVLRSENGSVWKEHRNRYEESYMDQLLSGMDEELESQEELEKKRVCRIITTDFPLYFVVMSRICQDCDMIGPEGGCLKSTLVPMVQATFPDTAVTKKVRLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWKDNPRDSGEGDTTSLRLLCSVIGGTAQAQWEDITGTTKLVYEKECANFTTNVSARFWLADCPRTAEAVHFATVLYKELTAVPYMAKFVVFAKMNDGREGRLRCYCMTDDKVDKTLEHHENFTEVARSRDIEVVEGMPLHVELSGNLVPVKKATQSRTFFFQSFRESRLAIPIKVRDSSREASGSLSFLRKAMKYEDLQHVLCHLNISIPPCTKGSSTEERRRTLTPLSLRSLSSTDRADQKMIDIAEQLGLSWAELARELQFGVDDINRIRVENPNSLLEQSIALLNLWVSREGKGVKIENLYTALRNIDRSEIISTLEGSGRQSRSMKGSWRYMDRDYSLSPSQMNGYASLQDELLSPASLHYMLPSPLRADQYWNEVAIMDAIPMAATEQDALMEMSDMQVWSSGLTPSLVTAEDSSLECSKAEDSDATSEGRFPGHLLTDAHVPDHMGSMDLVEDDTVDSDAMNGLIELLEQEEGQRPEGKMPASDHQLGTGEQNPESEVSFVSVQQKVQATIVPSPTVSHTVERSADRLRDWNAEGSFISCLQDLTEGSWQEGVTRRLGPAHTVAAGAQGQEQEQVLAPDVELMRVSSTEDSDWQPQHPAGGWPEEPDSRCFGQGNEVLHLPGEHVTEEQFTDEQGNIITKKVIRKVVHQLDLDTDDRKEHEDLILEGSLQEPQDLEAEADHFMYSILQRDGLGAKEEVQVRVPKLEVSGSRMGAQIVKRASLKRGKH

Target ORF information:

RefSeq Version	XM_025142840.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ankyrin 1 (ANK1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025142840.1

ORF Insert Sequence:

ATGGTGGTGG AGCTTCTGCA CAAGGAGATT GTTTTGGAGA CCACTACCAA GAAGGGAAAC 
ACAGCCCTGC ACATCGCTGC CCTCGCTGGA CAGCAGGACG TGGTCCGGGA ACTGGTGAAC 
TATGGGGCCA ATGTCAATGC TCAGTCGCAG AAGGGCTTCA CACCACTCTA CATGGCAGCG 
CAGGAAAACC ACCTGGAAGT TGTCAAATTC CTGCTGGAAA ACGGAGCCAA CCAGAATGTA 
GCCACAGAGG ATGGCTTCAC ACCACTAGCT GTGGCTCTGC AGCAGGGTCA TGAGAATGTG 
GTTGCTCACC TTATCAACTA TGGGACGAAG GGTAAGGTCC GCCTGCCTGC CTTGCACATT 
GCAGCTCGCA ACGATGACAC CCGTACAGCC GCCGTGCTGC TGCAGAATGA CCCCAATGCT 
GACGTCCTCT CCAAGACTGG ATTTACACCT TTGCACATTG CAGCCCACTA TGAGAATCTC 
AGCGTGGCCC AATTATTGCT GAATCGTGGA GCCAGCGTCA ACTTCACACC CCAGAATGGG 
ATCACTCCTT TGCACATAGC CTCTCGTCGA GGCAACATCA TCATGGTACG GCTGCTGCTG 
GACCGTGGGG CCCAGATAGA GACAAGGACC AAGGATGAGT TGACGCCTCT CCACTGTGCA 
GCTCGCAATG GACATGTGCG AATTGCAGAG ATCCTACTGG ACCATGGTGC TCCCATTCAA 
GCCAAAACAA AGAACGGTCT GTCACCGATC CACATGGCAG CACAGGGTGA CCACCTGGAC 
TGTGTGCGCC TACTCCTGCA GTACAGTGCT GAGATAGACG ACATCACCCT GGACCACCTG 
ACACCACTGC ATGTAGCTGC GCACTGTGGC CACCACCGTG TGGCCAAGCT GCTGGTGGAG 
AAGGGAGCCA AGCCCAACTC CAGAGCTCTG AATGGCTTCA CGCCTCTCCA CATTGCCTGC 
AAGAAGAACC ACATTCGGGT GATGGAGTTG CTTCTGAAGA CAGGAGCCTC CATTGATGCT 
GTCACAGAGT CTGGCCTGAC CCCCTTGCAT GTGGCTGCCT TCATGGGGCA CTTGCCCATT 
GTCAAAACCT TGCTGCAGCG TGGAGCATCT CCTAATGTGT CCAATGTGAA AGTGGAGACT 
CCCCTGCACA TGGCAGCCAG AGCTGGGCAC ATGGATGTGG CCAAGTATCT GATACAGAAC 
AAGGCCAAAA TCAACGCCAA GGCCAAGGAT GACCAGACTC CTCTGCACTG TGCTGCACGC 
ATTGGCCACA CCAGCATGGT CCAACTCCTG CTGGAGAACA ATGCCGACCC CAACCTGGCC 
ACTACAGCAG GGCACACGCC TCTGCACATC GCTGCCAGAG AGGGGCATGT AGACACAGCC 
TTGGCCCTGC TAGAGAAGGG AGCCTCTCAG ACCTGCATGA CCAAGAAAGG ATTTACCCCT 
CTCCACGTTG CAGCCAAGTA TGGGAAGGTG GATGTGGCAG AGCTGCTGCT GGTGCATGAT 
GCTCACCCCA ATGCAGCGGG GAAGAACGGC CTGACCCCAC TGCACGTAGC TGTGCACCAC 
AACAACCTGG AGATCGTCAA GCTTCTGCTT CCCAAGGGGA GCTCTCCACA CAGCTCAGCC 
TGGAACGGGT ACACCCCTCT GCACATTGCC GCCAAGCAGA ACCAGATGGA GGTGGCCAGC 
AGCTTGCTGC AGTATGGTGC TTCTGCAAAT GCGGAGTCTG TTCAGGGAGT CACCCCTCTG 
CACCTGGCTT CTCAGGAAGG GCATACAGAC ATGGTAGCAC TGCTTTTCTC CAAGCAAGCC 
AATGGAAACT TAGGCAACAA GAGTGGTCTG ACTCCTCTCC ATCTCGTGGC CCAAGAGGGA 
CACGTACCAG TGGCTGATGT TCTGGTGAAA CATGGAGTCA CAGTGGATGC GACAACCAGG 
ATGGGCTACA CCTCACTGCA CATAGCCAGC CACTATGGGA ACATCAAGTT GGTGAAGTTT 
CTGCTGCAGC ACCAGGCTGA TGTCAATGCT AAGACTAAGC TGGGCTACAC ACCCCTGCAC 
CAGGCGGCAC AGCAGGGCCA CACGGATGTG GTGACCCTGC TGCTGAAGCA TGGTGCCTCT 
CCCAACGAGA TCAGCACAAA TGGCACCACT CCCCTGGCCA TTGCGAAGCG CCTCGGTTAC 
ATTTCCGTCA CAGATGTGCT CAAGATAGTC ACAGAGGAGA CCACCATCCC ACCATTCAGT 
GACAAGTACC GCATGAGCTT CCCGGAGACT GTGGATGAGA TTCTGGATGT GTCGGAGGAT 
GAAGGCACTG CTCATGTCAC AGTAATGGAG GAGGAGCTGG TCCCACCGAA ACCCAGAACA 
CCTGAACCCA AGGAGCAGGA GGGCAAGAGG GAGATGCTGG AGTTTGTGAC CGTGACAACA 
CTGGAGCAAA TGGAGGAATC TGCAGCTGTC CCACAGGTCC CCTGCGTGCC CCCTGAGACT 
GTGGTGACCA TAGCAGAGGA GTCTGAGCAG ATGGGACCTG TGGAGACTGA GGCTGAGCAA 
GGCGGCCTGC TGCCTGCACC CTCGGTGTCC CCACAGGAGC CCTCCAAGGA GTTCGATGAG 
GACTCCTTGA TTCCCAGCAG CCCGGCCACC GAAACCTCGG ACAACATCAG CCCCGTGGCC 
AGCCCCGTTC ACACAGGGTT CCTGGTGAGC TTCATGGTGG ATGCGCGTGG CGGCTCCATG 
CGGGGCAGCC GGCACCACGG GCTGCGCGTG GTCATCCCTC CCCGTGCCTG TGCTGCACCC 
ACCCGCATCA CTTGCCGCCT GGTGAAGCCT CAGAAGCTGC CTGCCCCCCC ACCGCTGGCT 
GAGGAGGAGG GCCTGGCAAG CCGCATCATT GCACTGGGGC CCGCTGGCGC CCAGTTCCTC 
AGCCCCGTCA TTGTGGAGAT TCCTCACTTT GCCTCCTATG GACGTGGGGA CCGTGAGCTG 
GTGGTGTTGC GCAGTGAGAA TGGCTCTGTC TGGAAGGAGC ACCGCAACCG CTACGAGGAG 
AGCTACATGG ACCAACTGCT CAGTGGCATG GATGAGGAGC TGGAGAGCCA AGAGGAGCTG 
GAGAAGAAGA GGGTCTGCCG CATCATCACC ACTGACTTTC CTCTCTACTT CGTGGTCATG 
TCCCGGATTT GCCAGGACTG TGACATGATT GGCCCCGAGG GAGGGTGCTT GAAAAGCACA 
CTGGTGCCCA TGGTGCAGGC CACCTTCCCA GACACCGCTG TCACCAAGAA AGTGAGGCTG 
GCTCTGCAGG CGCAGCCCGT GCCTGATGAG CTGGTGACCA AGCTGCTGGG GAACCAGGCA 
ACCTTCAGCC CCATCGTCAC AGTGGAGCCA CGTCGGAGGA AGTTCCACCG CCCCATTGGC 
CTCCGCATCC CTCTGCCCCC ATCCTGGAAG GACAATCCCC GGGACAGCGG CGAGGGTGAC 
ACCACCAGCC TGCGCCTGCT CTGTTCTGTG ATAGGAGGGA CAGCACAAGC TCAGTGGGAA 
GACATCACAG GCACCACAAA GCTGGTCTAT GAAAAAGAGT GTGCTAACTT TACCACCAAT 
GTGTCTGCCA GGTTCTGGCT GGCTGACTGC CCGCGCACTG CTGAGGCTGT GCACTTTGCC 
ACCGTGCTGT ACAAGGAACT GACGGCTGTG CCCTACATGG CCAAATTTGT GGTGTTTGCT 
AAGATGAACG ATGGACGGGA AGGCCGGCTG CGCTGCTACT GCATGACTGA TGACAAGGTG 
GACAAGACTT TGGAGCATCA TGAGAACTTC ACTGAGGTGG CCCGCAGCAG GGACATTGAG 
GTGGTGGAAG GAATGCCTCT GCATGTCGAG CTCTCAGGGA ACCTGGTGCC CGTCAAGAAG 
GCCACGCAGT CACGCACCTT CTTCTTCCAG TCCTTCCGGG AAAGCCGCCT CGCCATCCCC 
ATCAAGGTCC GGGACAGCAG CCGGGAAGCC AGTGGCTCCC TGTCCTTCCT GCGCAAGGCC 
ATGAAGTATG AGGACTTGCA GCATGTGCTC TGCCACCTCA ACATCAGCAT TCCACCCTGC 
ACCAAGGGAA GCAGCACTGA GGAGCGAAGG AGGACACTGA CACCTTTGTC TCTGCGCTCT 
CTGAGCAGCA CCGACAGGGC AGACCAGAAG ATGATCGACA TTGCAGAGCA GCTTGGCCTC 
AGCTGGGCAG AGCTGGCACG TGAGCTGCAG TTTGGGGTGG ATGACATCAA CAGGATTCGT 
GTGGAGAACC CTAACTCCTT GCTGGAGCAG AGCATAGCCT TGCTTAACCT CTGGGTCAGC 
CGCGAGGGCA AGGGTGTCAA AATTGAGAAC CTGTACACAG CGCTGAGGAA CATTGACCGC 
AGCGAGATCA TCAGCACGTT GGAGGGCTCC GGCCGGCAGA GCCGCAGCAT GAAGGGCAGC 
TGGCGTTACA TGGACAGGGA CTATTCACTC TCGCCCTCCC AGATGAATGG TTACGCTTCG 
CTGCAGGACG AGCTGCTCTC CCCTGCCTCC CTGCATTATA TGCTGCCATC CCCTCTGCGT 
GCTGACCAGT ACTGGAACGA GGTGGCCATC ATGGACGCCA TCCCCATGGC CGCCACTGAG 
CAGGACGCCC TGATGGAGAT GTCTGACATG CAGGTGTGGT CCTCAGGGCT CACCCCTTCA 
CTGGTGACGG CTGAGGACTC CTCTCTGGAG TGCAGCAAGG CTGAGGACTC GGATGCTACA 
AGCGAAGGTC GTTTCCCAGG GCACCTTCTG ACAGATGCAC ATGTCCCAGA CCACATGGGC 
TCTATGGACT TAGTTGAGGA TGACACAGTG GACTCAGACG CCATGAACGG GCTGATTGAA 
CTGCTAGAGC AGGAGGAGGG GCAGAGGCCA GAGGGGAAGA TGCCAGCCAG CGACCACCAG 
CTAGGGACTG GAGAGCAGAA CCCAGAGAGT GAAGTCTCTT TTGTTTCAGT TCAACAGAAG 
GTACAAGCCA CGATCGTGCC ATCGCCAACC GTGAGCCACA CAGTGGAGAG GAGTGCAGAC 
AGGCTGAGGG ACTGGAATGC AGAAGGCTCC TTTATCTCCT GCCTACAGGA CCTGACAGAG 
GGCTCCTGGC AGGAGGGGGT CACCCGACGG CTGGGTCCAG CACACACCGT GGCTGCTGGG 
GCGCAGGGCC AGGAGCAAGA GCAGGTCCTG GCGCCGGACG TGGAGCTGAT GCGGGTCAGC 
TCCACAGAGG ACAGCGACTG GCAGCCCCAG CACCCCGCGG GCGGCTGGCC AGAGGAGCCT 
GACAGCCGCT GCTTTGGGCA GGGCAACGAA GTCCTCCATC TCCCTGGGGA GCATGTGACT 
GAGGAGCAGT TCACAGATGA ACAAGGCAAT ATAATCACCA AGAAGGTCAT CCGGAAAGTG 
GTGCATCAGT TGGACCTCGA CACGGATGAC AGGAAGGAGC ATGAGGACTT GATTCTGGAG 
GGCTCCCTGC AGGAGCCCCA AGACCTGGAG GCAGAGGCTG ATCACTTCAT GTACTCCATC 
CTACAGCGGG ACGGTCTGGG GGCCAAGGAG GAGGTGCAAG TGCGTGTCCC AAAACTGGAA 
GTCTCCGGGA GCAGGATGGG GGCTCAGATA GTGAAACGAG CCAGTCTGAA AAGGGGGAAG 
CATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK1 ( XM_015297397.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152883.1 Gallus gallus ankyrin 1 (ANK1), transcript variant X9, mRNA.

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CloneID	OGa147286
Clone ID Related Accession (Same CDS sequence)	XM_015297397.2
Accession Version	XM_015297397.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ankyrin-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006109.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015297397.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGAGAG GGCATGCACC AACCACTGTG GACCCCTTGC CTTCTTGTTG CAAGCAAGGA 
TCTCCTGAAG GGATGCTGGA GCAGGAGTCT GCTGTGCAGG CTGATGCTGC GACCAGCTTC 
CTGAGAGCTG CAAGATCTGG GAATCTGGAC AGAGCCTTGG ATCATCTCAG GAATGGGGTA 
GATATTAACA CCTGTAACCA GAATGGGCTG AATGCCTTGC ACCTGGCCTC CAAGGAGGGC 
CACACGAAAA TGGTGGTGGA GCTTCTGCAC AAGGAGATTG TTTTGGAGAC CACTACCAAG 
AAGGGAAACA CAGCCCTGCA CATCGCTGCC CTCGCTGGAC AGCAGGACGT GGTCCGGGAA 
CTGGTGAACT ATGGGGCCAA TGTCAATGCT CAGTCGCAGA AGGGCTTCAC ACCACTCTAC 
ATGGCAGCGC AGGAAAACCA CCTGGAAGTT GTCAAATTCC TGCTGGAAAA CGGAGCCAAC 
CAGAATGTAG CCACAGAGGA TGGCTTCACA CCACTAGCTG TGGCTCTGCA GCAGGGTCAT 
GAGAATGTGG TTGCTCACCT TATCAACTAT GGGACGAAGG GTAAGGTCCG CCTGCCTGCC 
TTGCACATTG CAGCTCGCAA CGATGACACC CGTACAGCCG CCGTGCTGCT GCAGAATGAC 
CCCAATGCTG ACGTCCTCTC CAAGACTGGA TTTACACCTT TGCACATTGC AGCCCACTAT 
GAGAATCTCA GCGTGGCCCA ATTATTGCTG AATCGTGGAG CCAGCGTCAA CTTCACACCC 
CAGAATGGGA TCACTCCTTT GCACATAGCC TCTCGTCGAG GCAACATCAT CATGGTACGG 
CTGCTGCTGG ACCGTGGGGC CCAGATAGAG ACAAGGACCA AGGATGAGTT GACGCCTCTC 
CACTGTGCAG CTCGCAATGG ACATGTGCGA ATTGCAGAGA TCCTACTGGA CCATGGTGCT 
CCCATTCAAG CCAAAACAAA GAACGGTCTG TCACCGATCC ACATGGCAGC ACAGGGTGAC 
CACCTGGACT GTGTGCGCCT ACTCCTGCAG TACAGTGCTG AGATAGACGA CATCACCCTG 
GACCACCTGA CACCACTGCA TGTAGCTGCG CACTGTGGCC ACCACCGTGT GGCCAAGCTG 
CTGGTGGAGA AGGGAGCCAA GCCCAACTCC AGAGCTCTGA ATGGCTTCAC GCCTCTCCAC 
ATTGCCTGCA AGAAGAACCA CATTCGGGTG ATGGAGTTGC TTCTGAAGAC AGGAGCCTCC 
ATTGATGCTG TCACAGAGTC TGGCCTGACC CCCTTGCATG TGGCTGCCTT CATGGGGCAC 
TTGCCCATTG TCAAAACCTT GCTGCAGCGT GGAGCATCTC CTAATGTGTC CAATGTGAAA 
GTGGAGACTC CCCTGCACAT GGCAGCCAGA GCTGGGCACA TGGATGTGGC CAAGTATCTG 
ATACAGAACA AGGCCAAAAT CAACGCCAAG GCCAAGGATG ACCAGACTCC TCTGCACTGT 
GCTGCACGCA TTGGCCACAC CAGCATGGTC CAACTCCTGC TGGAGAACAA TGCCGACCCC 
AACCTGGCCA CTACAGCAGG GCACACGCCT CTGCACATCG CTGCCAGAGA GGGGCATGTA 
GACACAGCCT TGGCCCTGCT AGAGAAGGGA GCCTCTCAGA CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TCCACGTTGC AGCCAAGTAT GGGAAGGTGG ATGTGGCAGA GCTGCTGCTG 
GTGCATGATG CTCACCCCAA TGCAGCGGGG AAGAACGGCC TGACCCCACT GCACGTAGCT 
GTGCACCACA ACAACCTGGA GATCGTCAAG CTTCTGCTTC CCAAGGGGAG CTCTCCACAC 
AGCTCAGCCT GGAACGGGTA CACCCCTCTG CACATTGCCG CCAAGCAGAA CCAGATGGAG 
GTGGCCAGCA GCTTGCTGCA GTATGGTGCT TCTGCAAATG CGGAGTCTGT TCAGGGAGTC 
ACCCCTCTGC ACCTGGCTTC TCAGGAAGGG CATACAGACA TGGTAGCACT GCTTTTCTCC 
AAGCAAGCCA ATGGAAACTT AGGCAACAAG AGTGGTCTGA CTCCTCTCCA TCTCGTGGCC 
CAAGAGGGAC ACGTACCAGT GGCTGATGTT CTGGTGAAAC ATGGAGTCAC AGTGGATGCG 
ACAACCAGGA TGGGCTACAC CTCACTGCAC ATAGCCAGCC ACTATGGGAA CATCAAGTTG 
GTGAAGTTTC TGCTGCAGCA CCAGGCTGAT GTCAATGCTA AGACTAAGCT GGGCTACACA 
CCCCTGCACC AGGCGGCACA GCAGGGCCAC ACGGATGTGG TGACCCTGCT GCTGAAGCAT 
GGTGCCTCTC CCAACGAGAT CAGCACAAAT GGCACCACTC CCCTGGCCAT TGCGAAGCGC 
CTCGGTTACA TTTCCGTCAC AGATGTGCTC AAGATAGTCA CAGAGGAGAC CACCATCCCA 
CCATTCAGTG ACAAGTACCG CATGAGCTTC CCGGAGACTG TGGATGAGAT TCTGGATGTG 
TCGGAGGATG AAGGCACTGC TCATGTCACA GTAATGGAGG AGGAGCTGGT CCCACCGAAA 
CCCAGAACAC CTGAACCCAA GGAGCAGGAG GGCAAGAGGG AGATGCTGGA GTTTGTGACC 
GTGACAACAC TGGAGCAAAT GGAGGAATCT GCAGCTGTCC CACAGGTCCC CTGCGTGCCC 
CCTGAGACTG TGGTGACCAT AGCAGAGGAG TCTGAGCAGA TGGGACCTGT GGAGACTGAG 
GCTGAGCAAG GCGGCCTGCT GCCTGCACCC TCGGTGTCCC CACAGGAGCC CTCCAAGGAG 
TTCGATGAGG ACTCCTTGAT TCCCAGCAGC CCGGCCACCG AAACCTCGGA CAACATCAGC 
CCCGTGGCCA GCCCCGTTCA CACAGGGTTC CTGGTGAGCT TCATGGTGGA TGCGCGTGGC 
GGCTCCATGC GGGGCAGCCG GCACCACGGG CTGCGCGTGG TCATCCCTCC CCGTGCCTGT 
GCTGCACCCA CCCGCATCAC TTGCCGCCTG GTGAAGCCTC AGAAGCTGCC TGCCCCCCCA 
CCGCTGGCTG AGGAGGAGGG CCTGGCAAGC CGCATCATTG CACTGGGGCC CGCTGGCGCC 
CAGTTCCTCA GCCCCGTCAT TGTGGAGATT CCTCACTTTG CCTCCTATGG ACGTGGGGAC 
CGTGAGCTGG TGGTGTTGCG CAGTGAGAAT GGCTCTGTCT GGAAGGAGCA CCGCAACCGC 
TACGAGGAGA GCTACATGGA CCAACTGCTC AGTGGCATGG ATGAGGAGCT GGAGAGCCAA 
GAGGAGCTGG AGAAGAAGAG GGTCTGCCGC ATCATCACCA CTGACTTTCC TCTCTACTTC 
GTGGTCATGT CCCGGATTTG CCAGGACTGT GACATGATTG GCCCCGAGGG AGGGTGCTTG 
AAAAGCACAC TGGTGCCCAT GGTGCAGGCC ACCTTCCCAG ACACCGCTGT CACCAAGAAA 
GTGAGGCTGG CTCTGCAGGC GCAGCCCGTG CCTGATGAGC TGGTGACCAA GCTGCTGGGG 
AACCAGGCAA CCTTCAGCCC CATCGTCACA GTGGAGCCAC GTCGGAGGAA GTTCCACCGC 
CCCATTGGCC TCCGCATCCC TCTGCCCCCA TCCTGGAAGG ACAATCCCCG GGACAGCGGC 
GAGGGTGACA CCACCAGCCT GCGCCTGCTC TGTTCTGTGA TAGGAGGGAC AGCACAAGCT 
CAGTGGGAAG ACATCACAGG CACCACAAAG CTGGTCTATG AAAAAGAGTG TGCTAACTTT 
ACCACCAATG TGTCTGCCAG GTTCTGGCTG GCTGACTGCC CGCGCACTGC TGAGGCTGTG 
CACTTTGCCA CCGTGCTGTA CAAGGAACTG ACGGCTGTGC CCTACATGGC CAAATTTGTG 
GTGTTTGCTA AGATGAACGA TGGACGGGAA GGCCGGCTGC GCTGCTACTG CATGACTGAT 
GACAAGGTGG ACAAGACTTT GGAGCATCAT GAGAACTTCA CTGAGGTGGC CCGCAGCAGG 
GACATTGAGG TGGTGGAAGG AATGCCTCTG CATGTCGAGC TCTCAGGGAA CCTGGTGCCC 
GTCAAGAAGG CCACGCAGTC ACGCACCTTC TTCTTCCAGT CCTTCCGGGA AAGCCGCCTC 
GCCATCCCCA TCAAGGTCCG GGACAGCAGC CGGGAAGCCA GTGGCTCCCT GTCCTTCCTG 
CGCAAGGCCA TGAAGTATGA GGACTTGCAG CATGTGCTCT GCCACCTCAA CATCAGCATT 
CCACCCTGCA CCAAGGGAAG CAGCACTGAG GAGCGAAGGA GGACACTGAC ACCTTTGTCT 
CTGCGCTCTC TGAGCAGCAC CGACAGGGCA GACCAGAAGA TGATCGACAT TGCAGAGCAG 
CTTGGCCTCA GCTGGGCAGA GCTGGCACGT GAGCTGCAGT TTGGGGTGGA TGACATCAAC 
AGGATTCGTG TGGAGAACCC TAACTCCTTG CTGGAGCAGA GCATAGCCTT GCTTAACCTC 
TGGGTCAGCC GCGAGGGCAA GGGTGTCAAA ATTGAGAACC TGTACACAGC GCTGAGGAAC 
ATTGACCGCA GCGAGATCAT CAGCACGTTG GAGGGCTCCG GCCGGCAGAG CCGCAGCATG 
AAGGGCAGCT GGCGTTACAT GGACAGGGAC TATTCACTCT CGCCCTCCCA GATGAATGTT 
CAACAGAAGG TACAAGCCAC GATCGTGCCA TCGCCAACCG TGAGCCACAC AGTGGAGAGG 
AGTGCAGACA GGCTGAGGGA CTGGAATGCA GAAGGCTCCT TTATCTCCTG CCTACAGGAC 
CTGACAGAGG GCTCCTGGCA GGAGGGGGTC ACCCGACGGC TGGGTCCAGC ACACACCGTG 
GCTGCTGGGG CGCAGGGCCA GGAGCAAGAG CAGGTCCTGG CGCCGGACGT GGAGCTGATG 
CGGGTCAGCT CCACAGAGGA CAGCGACTGG CAGCCCCAGC ACCCCGCGGG CGGCTGGCCA 
GAGGAGCCTG ACAGCCGCTG CTTTGGGCAG GGCAACGAAG TCCTCCATCT CCCTGGGGAG 
CATGTGACTG AGGAGCAGTT CACAGATGAA CAAGGCAATA TAATCACCAA GAAGGTCATC 
CGGAAAGTGG TGCATCAGTT GGACCTCGAC ACGGATGACA GGAAGGAGCA TGAGGACTTG 
ATTCTGGAGG GCTCCCTGCA GGAGCCCCAA GACCTGGAGG CAGAGGCTGA TCACTTCATG 
TACTCCATCC TACAGCGGGA CGGTCTGGGG GCCAAGGAGG AGGTGCAAGT GCGTGTCCCA 
AAACTGGAAG TCTCCGGGAG CAGGATGGGG GCTCAGATAG TGAAACGAGC CAGTCTGAAA 
AGGGGGAAGC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152883.1
CDS	831..6305
Translation	MAQAAKQLKKIKDIEAQALQEQKEKEESNRKRRNRSRDRKKKRGHAPTTVDPLPSCCKQGSPEGMLEQESAVQADAATSFLRAARSGNLDRALDHLRNGVDINTCNQNGLNALHLASKEGHTKMVVELLHKEIVLETTTKKGNTALHIAALAGQQDVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLSVAQLLLNRGASVNFTPQNGITPLHIASRRGNIIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRIAEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYSAEIDDITLDHLTPLHVAAHCGHHRVAKLLVEKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVAAFMGHLPIVKTLLQRGASPNVSNVKVETPLHMAARAGHMDVAKYLIQNKAKINAKAKDDQTPLHCAARIGHTSMVQLLLENNADPNLATTAGHTPLHIAAREGHVDTALALLEKGASQTCMTKKGFTPLHVAAKYGKVDVAELLLVHDAHPNAAGKNGLTPLHVAVHHNNLEIVKLLLPKGSSPHSSAWNGYTPLHIAAKQNQMEVASSLLQYGASANAESVQGVTPLHLASQEGHTDMVALLFSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLVKHGVTVDATTRMGYTSLHIASHYGNIKLVKFLLQHQADVNAKTKLGYTPLHQAAQQGHTDVVTLLLKHGASPNEISTNGTTPLAIAKRLGYISVTDVLKIVTEETTIPPFSDKYRMSFPETVDEILDVSEDEGTAHVTVMEEELVPPKPRTPEPKEQEGKREMLEFVTVTTLEQMEESAAVPQVPCVPPETVVTIAEESEQMGPVETEAEQGGLLPAPSVSPQEPSKEFDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHHGLRVVIPPRACAAPTRITCRLVKPQKLPAPPPLAEEEGLASRIIALGPAGAQFLSPVIVEIPHFASYGRGDRELVVLRSENGSVWKEHRNRYEESYMDQLLSGMDEELESQEELEKKRVCRIITTDFPLYFVVMSRICQDCDMIGPEGGCLKSTLVPMVQATFPDTAVTKKVRLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWKDNPRDSGEGDTTSLRLLCSVIGGTAQAQWEDITGTTKLVYEKECANFTTNVSARFWLADCPRTAEAVHFATVLYKELTAVPYMAKFVVFAKMNDGREGRLRCYCMTDDKVDKTLEHHENFTEVARSRDIEVVEGMPLHVELSGNLVPVKKATQSRTFFFQSFRESRLAIPIKVRDSSREASGSLSFLRKAMKYEDLQHVLCHLNISIPPCTKGSSTEERRRTLTPLSLRSLSSTDRADQKMIDIAEQLGLSWAELARELQFGVDDINRIRVENPNSLLEQSIALLNLWVSREGKGVKIENLYTALRNIDRSEIISTLEGSGRQSRSMKGSWRYMDRDYSLSPSQMNVQQKVQATIVPSPTVSHTVERSADRLRDWNAEGSFISCLQDLTEGSWQEGVTRRLGPAHTVAAGAQGQEQEQVLAPDVELMRVSSTEDSDWQPQHPAGGWPEEPDSRCFGQGNEVLHLPGEHVTEEQFTDEQGNIITKKVIRKVVHQLDLDTDDRKEHEDLILEGSLQEPQDLEAEADHFMYSILQRDGLGAKEEVQVRVPKLEVSGSRMGAQIVKRASLKRGKH

Target ORF information:

RefSeq Version	XM_015297397.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ankyrin 1 (ANK1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297397.2

ORF Insert Sequence:

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGAGAG GGCATGCACC AACCACTGTG GACCCCTTGC CTTCTTGTTG CAAGCAAGGA 
TCTCCTGAAG GGATGCTGGA GCAGGAGTCT GCTGTGCAGG CTGATGCTGC GACCAGCTTC 
CTGAGAGCTG CAAGATCTGG GAATCTGGAC AGAGCCTTGG ATCATCTCAG GAATGGGGTA 
GATATTAACA CCTGTAACCA GAATGGGCTG AATGCCTTGC ACCTGGCCTC CAAGGAGGGC 
CACACGAAAA TGGTGGTGGA GCTTCTGCAC AAGGAGATTG TTTTGGAGAC CACTACCAAG 
AAGGGAAACA CAGCCCTGCA CATCGCTGCC CTCGCTGGAC AGCAGGACGT GGTCCGGGAA 
CTGGTGAACT ATGGGGCCAA TGTCAATGCT CAGTCGCAGA AGGGCTTCAC ACCACTCTAC 
ATGGCAGCGC AGGAAAACCA CCTGGAAGTT GTCAAATTCC TGCTGGAAAA CGGAGCCAAC 
CAGAATGTAG CCACAGAGGA TGGCTTCACA CCACTAGCTG TGGCTCTGCA GCAGGGTCAT 
GAGAATGTGG TTGCTCACCT TATCAACTAT GGGACGAAGG GTAAGGTCCG CCTGCCTGCC 
TTGCACATTG CAGCTCGCAA CGATGACACC CGTACAGCCG CCGTGCTGCT GCAGAATGAC 
CCCAATGCTG ACGTCCTCTC CAAGACTGGA TTTACACCTT TGCACATTGC AGCCCACTAT 
GAGAATCTCA GCGTGGCCCA ATTATTGCTG AATCGTGGAG CCAGCGTCAA CTTCACACCC 
CAGAATGGGA TCACTCCTTT GCACATAGCC TCTCGTCGAG GCAACATCAT CATGGTACGG 
CTGCTGCTGG ACCGTGGGGC CCAGATAGAG ACAAGGACCA AGGATGAGTT GACGCCTCTC 
CACTGTGCAG CTCGCAATGG ACATGTGCGA ATTGCAGAGA TCCTACTGGA CCATGGTGCT 
CCCATTCAAG CCAAAACAAA GAACGGTCTG TCACCGATCC ACATGGCAGC ACAGGGTGAC 
CACCTGGACT GTGTGCGCCT ACTCCTGCAG TACAGTGCTG AGATAGACGA CATCACCCTG 
GACCACCTGA CACCACTGCA TGTAGCTGCG CACTGTGGCC ACCACCGTGT GGCCAAGCTG 
CTGGTGGAGA AGGGAGCCAA GCCCAACTCC AGAGCTCTGA ATGGCTTCAC GCCTCTCCAC 
ATTGCCTGCA AGAAGAACCA CATTCGGGTG ATGGAGTTGC TTCTGAAGAC AGGAGCCTCC 
ATTGATGCTG TCACAGAGTC TGGCCTGACC CCCTTGCATG TGGCTGCCTT CATGGGGCAC 
TTGCCCATTG TCAAAACCTT GCTGCAGCGT GGAGCATCTC CTAATGTGTC CAATGTGAAA 
GTGGAGACTC CCCTGCACAT GGCAGCCAGA GCTGGGCACA TGGATGTGGC CAAGTATCTG 
ATACAGAACA AGGCCAAAAT CAACGCCAAG GCCAAGGATG ACCAGACTCC TCTGCACTGT 
GCTGCACGCA TTGGCCACAC CAGCATGGTC CAACTCCTGC TGGAGAACAA TGCCGACCCC 
AACCTGGCCA CTACAGCAGG GCACACGCCT CTGCACATCG CTGCCAGAGA GGGGCATGTA 
GACACAGCCT TGGCCCTGCT AGAGAAGGGA GCCTCTCAGA CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TCCACGTTGC AGCCAAGTAT GGGAAGGTGG ATGTGGCAGA GCTGCTGCTG 
GTGCATGATG CTCACCCCAA TGCAGCGGGG AAGAACGGCC TGACCCCACT GCACGTAGCT 
GTGCACCACA ACAACCTGGA GATCGTCAAG CTTCTGCTTC CCAAGGGGAG CTCTCCACAC 
AGCTCAGCCT GGAACGGGTA CACCCCTCTG CACATTGCCG CCAAGCAGAA CCAGATGGAG 
GTGGCCAGCA GCTTGCTGCA GTATGGTGCT TCTGCAAATG CGGAGTCTGT TCAGGGAGTC 
ACCCCTCTGC ACCTGGCTTC TCAGGAAGGG CATACAGACA TGGTAGCACT GCTTTTCTCC 
AAGCAAGCCA ATGGAAACTT AGGCAACAAG AGTGGTCTGA CTCCTCTCCA TCTCGTGGCC 
CAAGAGGGAC ACGTACCAGT GGCTGATGTT CTGGTGAAAC ATGGAGTCAC AGTGGATGCG 
ACAACCAGGA TGGGCTACAC CTCACTGCAC ATAGCCAGCC ACTATGGGAA CATCAAGTTG 
GTGAAGTTTC TGCTGCAGCA CCAGGCTGAT GTCAATGCTA AGACTAAGCT GGGCTACACA 
CCCCTGCACC AGGCGGCACA GCAGGGCCAC ACGGATGTGG TGACCCTGCT GCTGAAGCAT 
GGTGCCTCTC CCAACGAGAT CAGCACAAAT GGCACCACTC CCCTGGCCAT TGCGAAGCGC 
CTCGGTTACA TTTCCGTCAC AGATGTGCTC AAGATAGTCA CAGAGGAGAC CACCATCCCA 
CCATTCAGTG ACAAGTACCG CATGAGCTTC CCGGAGACTG TGGATGAGAT TCTGGATGTG 
TCGGAGGATG AAGGCACTGC TCATGTCACA GTAATGGAGG AGGAGCTGGT CCCACCGAAA 
CCCAGAACAC CTGAACCCAA GGAGCAGGAG GGCAAGAGGG AGATGCTGGA GTTTGTGACC 
GTGACAACAC TGGAGCAAAT GGAGGAATCT GCAGCTGTCC CACAGGTCCC CTGCGTGCCC 
CCTGAGACTG TGGTGACCAT AGCAGAGGAG TCTGAGCAGA TGGGACCTGT GGAGACTGAG 
GCTGAGCAAG GCGGCCTGCT GCCTGCACCC TCGGTGTCCC CACAGGAGCC CTCCAAGGAG 
TTCGATGAGG ACTCCTTGAT TCCCAGCAGC CCGGCCACCG AAACCTCGGA CAACATCAGC 
CCCGTGGCCA GCCCCGTTCA CACAGGGTTC CTGGTGAGCT TCATGGTGGA TGCGCGTGGC 
GGCTCCATGC GGGGCAGCCG GCACCACGGG CTGCGCGTGG TCATCCCTCC CCGTGCCTGT 
GCTGCACCCA CCCGCATCAC TTGCCGCCTG GTGAAGCCTC AGAAGCTGCC TGCCCCCCCA 
CCGCTGGCTG AGGAGGAGGG CCTGGCAAGC CGCATCATTG CACTGGGGCC CGCTGGCGCC 
CAGTTCCTCA GCCCCGTCAT TGTGGAGATT CCTCACTTTG CCTCCTATGG ACGTGGGGAC 
CGTGAGCTGG TGGTGTTGCG CAGTGAGAAT GGCTCTGTCT GGAAGGAGCA CCGCAACCGC 
TACGAGGAGA GCTACATGGA CCAACTGCTC AGTGGCATGG ATGAGGAGCT GGAGAGCCAA 
GAGGAGCTGG AGAAGAAGAG GGTCTGCCGC ATCATCACCA CTGACTTTCC TCTCTACTTC 
GTGGTCATGT CCCGGATTTG CCAGGACTGT GACATGATTG GCCCCGAGGG AGGGTGCTTG 
AAAAGCACAC TGGTGCCCAT GGTGCAGGCC ACCTTCCCAG ACACCGCTGT CACCAAGAAA 
GTGAGGCTGG CTCTGCAGGC GCAGCCCGTG CCTGATGAGC TGGTGACCAA GCTGCTGGGG 
AACCAGGCAA CCTTCAGCCC CATCGTCACA GTGGAGCCAC GTCGGAGGAA GTTCCACCGC 
CCCATTGGCC TCCGCATCCC TCTGCCCCCA TCCTGGAAGG ACAATCCCCG GGACAGCGGC 
GAGGGTGACA CCACCAGCCT GCGCCTGCTC TGTTCTGTGA TAGGAGGGAC AGCACAAGCT 
CAGTGGGAAG ACATCACAGG CACCACAAAG CTGGTCTATG AAAAAGAGTG TGCTAACTTT 
ACCACCAATG TGTCTGCCAG GTTCTGGCTG GCTGACTGCC CGCGCACTGC TGAGGCTGTG 
CACTTTGCCA CCGTGCTGTA CAAGGAACTG ACGGCTGTGC CCTACATGGC CAAATTTGTG 
GTGTTTGCTA AGATGAACGA TGGACGGGAA GGCCGGCTGC GCTGCTACTG CATGACTGAT 
GACAAGGTGG ACAAGACTTT GGAGCATCAT GAGAACTTCA CTGAGGTGGC CCGCAGCAGG 
GACATTGAGG TGGTGGAAGG AATGCCTCTG CATGTCGAGC TCTCAGGGAA CCTGGTGCCC 
GTCAAGAAGG CCACGCAGTC ACGCACCTTC TTCTTCCAGT CCTTCCGGGA AAGCCGCCTC 
GCCATCCCCA TCAAGGTCCG GGACAGCAGC CGGGAAGCCA GTGGCTCCCT GTCCTTCCTG 
CGCAAGGCCA TGAAGTATGA GGACTTGCAG CATGTGCTCT GCCACCTCAA CATCAGCATT 
CCACCCTGCA CCAAGGGAAG CAGCACTGAG GAGCGAAGGA GGACACTGAC ACCTTTGTCT 
CTGCGCTCTC TGAGCAGCAC CGACAGGGCA GACCAGAAGA TGATCGACAT TGCAGAGCAG 
CTTGGCCTCA GCTGGGCAGA GCTGGCACGT GAGCTGCAGT TTGGGGTGGA TGACATCAAC 
AGGATTCGTG TGGAGAACCC TAACTCCTTG CTGGAGCAGA GCATAGCCTT GCTTAACCTC 
TGGGTCAGCC GCGAGGGCAA GGGTGTCAAA ATTGAGAACC TGTACACAGC GCTGAGGAAC 
ATTGACCGCA GCGAGATCAT CAGCACGTTG GAGGGCTCCG GCCGGCAGAG CCGCAGCATG 
AAGGGCAGCT GGCGTTACAT GGACAGGGAC TATTCACTCT CGCCCTCCCA GATGAATGTT 
CAACAGAAGG TACAAGCCAC GATCGTGCCA TCGCCAACCG TGAGCCACAC AGTGGAGAGG 
AGTGCAGACA GGCTGAGGGA CTGGAATGCA GAAGGCTCCT TTATCTCCTG CCTACAGGAC 
CTGACAGAGG GCTCCTGGCA GGAGGGGGTC ACCCGACGGC TGGGTCCAGC ACACACCGTG 
GCTGCTGGGG CGCAGGGCCA GGAGCAAGAG CAGGTCCTGG CGCCGGACGT GGAGCTGATG 
CGGGTCAGCT CCACAGAGGA CAGCGACTGG CAGCCCCAGC ACCCCGCGGG CGGCTGGCCA 
GAGGAGCCTG ACAGCCGCTG CTTTGGGCAG GGCAACGAAG TCCTCCATCT CCCTGGGGAG 
CATGTGACTG AGGAGCAGTT CACAGATGAA CAAGGCAATA TAATCACCAA GAAGGTCATC 
CGGAAAGTGG TGCATCAGTT GGACCTCGAC ACGGATGACA GGAAGGAGCA TGAGGACTTG 
ATTCTGGAGG GCTCCCTGCA GGAGCCCCAA GACCTGGAGG CAGAGGCTGA TCACTTCATG 
TACTCCATCC TACAGCGGGA CGGTCTGGGG GCCAAGGAGG AGGTGCAAGT GCGTGTCCCA 
AAACTGGAAG TCTCCGGGAG CAGGATGGGG GCTCAGATAG TGAAACGAGC CAGTCTGAAA 
AGGGGGAAGC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK1 ( XM_015297399.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152885.1 Gallus gallus ankyrin 1 (ANK1), transcript variant X11, mRNA.

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CloneID	OGa147287
Clone ID Related Accession (Same CDS sequence)	XM_015297399.2
Accession Version	XM_015297399.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ankyrin-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006109.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015297399.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGAGAG GGCATGCACC AACCACTGTG GACCCCTTGC CTTCTTGTTG CAAGCAAGGA 
TCTCCTGAAG GGATGCTGGA GCAGGAGTCT GCTGTGCAGG CTGATGCTGC GACCAGCTTC 
CTGAGAGCTG CAAGATCTGG GAATCTGGAC AGAGCCTTGG ATCATCTCAG GAATGGGGTA 
GATATTAACA CCTGTAACCA GAATGGGCTG AATGCCTTGC ACCTGGCCTC CAAGGAGGGC 
CACACGAAAA TGGTGGTGGA GCTTCTGCAC AAGGAGATTG TTTTGGAGAC CACTACCAAG 
AAGGGAAACA CAGCCCTGCA CATCGCTGCC CTCGCTGGAC AGCAGGACGT GGTCCGGGAA 
CTGGTGAACT ATGGGGCCAA TGTCAATGCT CAGTCGCAGA AGGGCTTCAC ACCACTCTAC 
ATGGCAGCGC AGGAAAACCA CCTGGAAGTT GTCAAATTCC TGCTGGAAAA CGGAGCCAAC 
CAGAATGTAG CCACAGAGGA TGGCTTCACA CCACTAGCTG TGGCTCTGCA GCAGGGTCAT 
GAGAATGTGG TTGCTCACCT TATCAACTAT GGGACGAAGG GTAAGGTCCG CCTGCCTGCC 
TTGCACATTG CAGCTCGCAA CGATGACACC CGTACAGCCG CCGTGCTGCT GCAGAATGAC 
CCCAATGCTG ACGTCCTCTC CAAGACTGGA TTTACACCTT TGCACATTGC AGCCCACTAT 
GAGAATCTCA GCGTGGCCCA ATTATTGCTG AATCGTGGAG CCAGCGTCAA CTTCACACCC 
CAGAATGGGA TCACTCCTTT GCACATAGCC TCTCGTCGAG GCAACATCAT CATGGTACGG 
CTGCTGCTGG ACCGTGGGGC CCAGATAGAG ACAAGGACCA AGGATGAGTT GACGCCTCTC 
CACTGTGCAG CTCGCAATGG ACATGTGCGA ATTGCAGAGA TCCTACTGGA CCATGGTGCT 
CCCATTCAAG CCAAAACAAA GAACGGTCTG TCACCGATCC ACATGGCAGC ACAGGGTGAC 
CACCTGGACT GTGTGCGCCT ACTCCTGCAG TACAGTGCTG AGATAGACGA CATCACCCTG 
GACCACCTGA CACCACTGCA TGTAGCTGCG CACTGTGGCC ACCACCGTGT GGCCAAGCTG 
CTGGTGGAGA AGGGAGCCAA GCCCAACTCC AGAGCTCTGA ATGGCTTCAC GCCTCTCCAC 
ATTGCCTGCA AGAAGAACCA CATTCGGGTG ATGGAGTTGC TTCTGAAGAC AGGAGCCTCC 
ATTGATGCTG TCACAGAGTC TGGCCTGACC CCCTTGCATG TGGCTGCCTT CATGGGGCAC 
TTGCCCATTG TCAAAACCTT GCTGCAGCGT GGAGCATCTC CTAATGTGTC CAATGTGAAA 
GTGGAGACTC CCCTGCACAT GGCAGCCAGA GCTGGGCACA TGGATGTGGC CAAGTATCTG 
ATACAGAACA AGGCCAAAAT CAACGCCAAG GCCAAGGATG ACCAGACTCC TCTGCACTGT 
GCTGCACGCA TTGGCCACAC CAGCATGGTC CAACTCCTGC TGGAGAACAA TGCCGACCCC 
AACCTGGCCA CTACAGCAGG GCACACGCCT CTGCACATCG CTGCCAGAGA GGGGCATGTA 
GACACAGCCT TGGCCCTGCT AGAGAAGGGA GCCTCTCAGA CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TCCACGTTGC AGCCAAGTAT GGGAAGGTGG ATGTGGCAGA GCTGCTGCTG 
GTGCATGATG CTCACCCCAA TGCAGCGGGG AAGAACGGCC TGACCCCACT GCACGTAGCT 
GTGCACCACA ACAACCTGGA GATCGTCAAG CTTCTGCTTC CCAAGGGGAG CTCTCCACAC 
AGCTCAGCCT GGAACGGGTA CACCCCTCTG CACATTGCCG CCAAGCAGAA CCAGATGGAG 
GTGGCCAGCA GCTTGCTGCA GTATGGTGCT TCTGCAAATG CGGAGTCTGT TCAGGGAGTC 
ACCCCTCTGC ACCTGGCTTC TCAGGAAGGG CATACAGACA TGGTAGCACT GCTTTTCTCC 
AAGCAAGCCA ATGGAAACTT AGGCAACAAG AGTGGTCTGA CTCCTCTCCA TCTCGTGGCC 
CAAGAGGGAC ACGTACCAGT GGCTGATGTT CTGGTGAAAC ATGGAGTCAC AGTGGATGCG 
ACAACCAGGA TGGGCTACAC CTCACTGCAC ATAGCCAGCC ACTATGGGAA CATCAAGTTG 
GTGAAGTTTC TGCTGCAGCA CCAGGCTGAT GTCAATGCTA AGACTAAGCT GGGCTACACA 
CCCCTGCACC AGGCGGCACA GCAGGGCCAC ACGGATGTGG TGACCCTGCT GCTGAAGCAT 
GGTGCCTCTC CCAACGAGAT CAGCACAAAT GGCACCACTC CCCTGGCCAT TGCGAAGCGC 
CTCGGTTACA TTTCCGTCAC AGATGTGCTC AAGATAGTCA CAGAGGAGAC CACCATCCCA 
CCATTCAGTG ACAAGTACCG CATGAGCTTC CCGGAGACTG TGGATGAGAT TCTGGATGTG 
TCGGAGGATG AAGGCACTGC TCATGTCACA GTAATGGAGG AGGAGCTGGT CCCACCGAAA 
CCCAGAACAC CTGAACCCAA GGAGCAGGAG GGCAAGAGGG AGATGCTGGA GTTTGTGACC 
GTGACAACAC TGGAGCAAAT GGAGGAATCT GCAGCTGTCC CACAGGTCCC CTGCGTGCCC 
CCTGAGACTG TGGTGACCAT AGCAGAGGAG TCTGAGCAGA TGGGACCTGT GGAGACTGAG 
GCTGAGCAAG GCGGCCTGCT GCCTGCACCC TCGGTGTCCC CACAGGAGCC CTCCAAGGAG 
TTCGATGAGG ACTCCTTGAT TCCCAGCAGC CCGGCCACCG AAACCTCGGA CAACATCAGC 
CCCGTGGCCA GCCCCGTTCA CACAGGGTTC CTGGTGAGCT TCATGGTGGA TGCGCGTGGC 
GGCTCCATGC GGGGCAGCCG GCACCACGGG CTGCGCGTGG TCATCCCTCC CCGTGCCTGT 
GCTGCACCCA CCCGCATCAC TTGCCGCCTG GTGAAGCCTC AGAAGCTGCC TGCCCCCCCA 
CCGCTGGCTG AGGAGGAGGG CCTGGCAAGC CGCATCATTG CACTGGGGCC CGCTGGCGCC 
CAGTTCCTCA GCCCCGTCAT TGTGGAGATT CCTCACTTTG CCTCCTATGG ACGTGGGGAC 
CGTGAGCTGG TGGTGTTGCG CAGTGAGAAT GGCTCTGTCT GGAAGGAGCA CCGCAACCGC 
TACGAGGAGA GCTACATGGA CCAACTGCTC AGTGGCATGG ATGAGGAGCT GGAGAGCCAA 
GAGGAGCTGG AGAAGAAGAG GGTCTGCCGC ATCATCACCA CTGACTTTCC TCTCTACTTC 
GTGGTCATGT CCCGGATTTG CCAGGACTGT GACATGATTG GCCCCGAGGG AGGGTGCTTG 
AAAAGCACAC TGGTGCCCAT GGTGCAGGCC ACCTTCCCAG ACACCGCTGT CACCAAGAAA 
GTGAGGCTGG CTCTGCAGGC GCAGCCCGTG CCTGATGAGC TGGTGACCAA GCTGCTGGGG 
AACCAGGCAA CCTTCAGCCC CATCGTCACA GTGGAGCCAC GTCGGAGGAA GTTCCACCGC 
CCCATTGGCC TCCGCATCCC TCTGCCCCCA TCCTGGAAGG ACAATCCCCG GGACAGCGGC 
GAGGGTGACA CCACCAGCCT GCGCCTGCTC TGTTCTGTGA TAGGAGGGAC AGCACAAGCT 
CAGTGGGAAG ACATCACAGG CACCACAAAG CTGGTCTATG AAAAAGAGTG TGCTAACTTT 
ACCACCAATG TGTCTGCCAG GTTCTGGCTG GCTGACTGCC CGCGCACTGC TGAGGCTGTG 
CACTTTGCCA CCGTGCTGTA CAAGGAACTG ACGGCTGTGC CCTACATGGC CAAATTTGTG 
GTGTTTGCTA AGATGAACGA TGGACGGGAA GGCCGGCTGC GCTGCTACTG CATGACTGAT 
GACAAGGTGG ACAAGACTTT GGAGCATCAT GAGAACTTCA CTGAGGTGGC CCGCAGCAGG 
GACATTGAGG TGGTGGAAGG AATGCCTCTG CATGTCGAGC TCTCAGGGAA CCTGGTGCCC 
GTCAAGAAGG CCACGCAGTC ACGCACCTTC TTCTTCCAGT CCTTCCGGGA AAGCCGCCTC 
GCCATCCCCA TCAAGGTCCG GGACAGCAGC CGGGAAGCCA GTGGCTCCCT GTCCTTCCTG 
CGCAAGGCCA TGAAGTATGA GGACTTGCAG CATGTGCTCT GCCACCTCAA CATCAGCATT 
CCACCCTGCA CCAAGGGAAG CAGCACTGAG GAGCGAAGGA GGACACTGAC ACCTTTGTCT 
CTGCGCTCTC TGAGCAGCAC CGACAGGGCA GACCAGAAGA TGATCGACAT TGCAGAGCAG 
CTTGGCCTCA GCTGGGCAGA GCTGGCACGT GAGCTGCAGT TTGGGGTGGA TGACATCAAC 
AGGATTCGTG TGGAGAACCC TAACTCCTTG CTGGAGCAGA GCATAGCCTT GCTTAACCTC 
TGGGTCAGCC GCGAGGGCAA GGGTGTCAAA ATTGAGAACC TGTACACAGC GCTGAGGAAC 
ATTGACCGCA GCGAGATCAT CAGCACGTTG GAGGGCTCCG GCCGGCAGAG CCGCAGCATG 
AAGGGCAGCT GGCGTTACAT GGACAGGGAC TATTCACTCT CGCCCTCCCA GATGAATGGT 
TACGCTTCGC TGCAGGACGA GCTGCTCTCC CCTGCCTCCC TGCATTATAT GCTGCCATCC 
CCTCTGCGTG CTGACCAGTA CTGGAACGAG GTGGCCATCA TGGACGCCAT CCCCATGGCC 
GCCACTGAGC AGGACGCCCT GATGGAGATG TCTGACATGC AGGTGTGGTC CTCAGGGCTC 
ACCCCTTCAC TGGTGACGGC TGAGGACTCC TCTCTGGAGT GCAGCAAGGC TGAGGACTCG 
GATGCTACAA GCGAAGGTCG TTTCCCAGGG CACCTTCTGA CAGATGCACA TGTCCCAGAC 
CACATGGGCT CTATGGACTT AGTTGAGGAT GACACAGTGG ACTCAGACGC CATGAACGGG 
CTGATTGAAC TGCTAGAGCA GGAGGAGGGG CAGAGGCCAG AGGGGAAGAT GCCAGCCAGC 
GACCACCAGC TAGGGACTGG AGAGCAGAAC CCAGAGAGTG AAGTCTCTTT TGTTTCAGTT 
CAACAGAAGG TACAAGCCAC GATCGTGCCA TCGCCAACCG TGAGCCACAC AGTGGAGAGG 
AGTGCAGACA GGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152885.1
CDS	839..6196
Translation	MAQAAKQLKKIKDIEAQALQEQKEKEESNRKRRNRSRDRKKKRGHAPTTVDPLPSCCKQGSPEGMLEQESAVQADAATSFLRAARSGNLDRALDHLRNGVDINTCNQNGLNALHLASKEGHTKMVVELLHKEIVLETTTKKGNTALHIAALAGQQDVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLSVAQLLLNRGASVNFTPQNGITPLHIASRRGNIIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRIAEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYSAEIDDITLDHLTPLHVAAHCGHHRVAKLLVEKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVAAFMGHLPIVKTLLQRGASPNVSNVKVETPLHMAARAGHMDVAKYLIQNKAKINAKAKDDQTPLHCAARIGHTSMVQLLLENNADPNLATTAGHTPLHIAAREGHVDTALALLEKGASQTCMTKKGFTPLHVAAKYGKVDVAELLLVHDAHPNAAGKNGLTPLHVAVHHNNLEIVKLLLPKGSSPHSSAWNGYTPLHIAAKQNQMEVASSLLQYGASANAESVQGVTPLHLASQEGHTDMVALLFSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLVKHGVTVDATTRMGYTSLHIASHYGNIKLVKFLLQHQADVNAKTKLGYTPLHQAAQQGHTDVVTLLLKHGASPNEISTNGTTPLAIAKRLGYISVTDVLKIVTEETTIPPFSDKYRMSFPETVDEILDVSEDEGTAHVTVMEEELVPPKPRTPEPKEQEGKREMLEFVTVTTLEQMEESAAVPQVPCVPPETVVTIAEESEQMGPVETEAEQGGLLPAPSVSPQEPSKEFDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHHGLRVVIPPRACAAPTRITCRLVKPQKLPAPPPLAEEEGLASRIIALGPAGAQFLSPVIVEIPHFASYGRGDRELVVLRSENGSVWKEHRNRYEESYMDQLLSGMDEELESQEELEKKRVCRIITTDFPLYFVVMSRICQDCDMIGPEGGCLKSTLVPMVQATFPDTAVTKKVRLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWKDNPRDSGEGDTTSLRLLCSVIGGTAQAQWEDITGTTKLVYEKECANFTTNVSARFWLADCPRTAEAVHFATVLYKELTAVPYMAKFVVFAKMNDGREGRLRCYCMTDDKVDKTLEHHENFTEVARSRDIEVVEGMPLHVELSGNLVPVKKATQSRTFFFQSFRESRLAIPIKVRDSSREASGSLSFLRKAMKYEDLQHVLCHLNISIPPCTKGSSTEERRRTLTPLSLRSLSSTDRADQKMIDIAEQLGLSWAELARELQFGVDDINRIRVENPNSLLEQSIALLNLWVSREGKGVKIENLYTALRNIDRSEIISTLEGSGRQSRSMKGSWRYMDRDYSLSPSQMNGYASLQDELLSPASLHYMLPSPLRADQYWNEVAIMDAIPMAATEQDALMEMSDMQVWSSGLTPSLVTAEDSSLECSKAEDSDATSEGRFPGHLLTDAHVPDHMGSMDLVEDDTVDSDAMNGLIELLEQEEGQRPEGKMPASDHQLGTGEQNPESEVSFVSVQQKVQATIVPSPTVSHTVERSADRT

Target ORF information:

RefSeq Version	XM_015297399.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ankyrin 1 (ANK1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297399.2

ORF Insert Sequence:

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGAGAG GGCATGCACC AACCACTGTG GACCCCTTGC CTTCTTGTTG CAAGCAAGGA 
TCTCCTGAAG GGATGCTGGA GCAGGAGTCT GCTGTGCAGG CTGATGCTGC GACCAGCTTC 
CTGAGAGCTG CAAGATCTGG GAATCTGGAC AGAGCCTTGG ATCATCTCAG GAATGGGGTA 
GATATTAACA CCTGTAACCA GAATGGGCTG AATGCCTTGC ACCTGGCCTC CAAGGAGGGC 
CACACGAAAA TGGTGGTGGA GCTTCTGCAC AAGGAGATTG TTTTGGAGAC CACTACCAAG 
AAGGGAAACA CAGCCCTGCA CATCGCTGCC CTCGCTGGAC AGCAGGACGT GGTCCGGGAA 
CTGGTGAACT ATGGGGCCAA TGTCAATGCT CAGTCGCAGA AGGGCTTCAC ACCACTCTAC 
ATGGCAGCGC AGGAAAACCA CCTGGAAGTT GTCAAATTCC TGCTGGAAAA CGGAGCCAAC 
CAGAATGTAG CCACAGAGGA TGGCTTCACA CCACTAGCTG TGGCTCTGCA GCAGGGTCAT 
GAGAATGTGG TTGCTCACCT TATCAACTAT GGGACGAAGG GTAAGGTCCG CCTGCCTGCC 
TTGCACATTG CAGCTCGCAA CGATGACACC CGTACAGCCG CCGTGCTGCT GCAGAATGAC 
CCCAATGCTG ACGTCCTCTC CAAGACTGGA TTTACACCTT TGCACATTGC AGCCCACTAT 
GAGAATCTCA GCGTGGCCCA ATTATTGCTG AATCGTGGAG CCAGCGTCAA CTTCACACCC 
CAGAATGGGA TCACTCCTTT GCACATAGCC TCTCGTCGAG GCAACATCAT CATGGTACGG 
CTGCTGCTGG ACCGTGGGGC CCAGATAGAG ACAAGGACCA AGGATGAGTT GACGCCTCTC 
CACTGTGCAG CTCGCAATGG ACATGTGCGA ATTGCAGAGA TCCTACTGGA CCATGGTGCT 
CCCATTCAAG CCAAAACAAA GAACGGTCTG TCACCGATCC ACATGGCAGC ACAGGGTGAC 
CACCTGGACT GTGTGCGCCT ACTCCTGCAG TACAGTGCTG AGATAGACGA CATCACCCTG 
GACCACCTGA CACCACTGCA TGTAGCTGCG CACTGTGGCC ACCACCGTGT GGCCAAGCTG 
CTGGTGGAGA AGGGAGCCAA GCCCAACTCC AGAGCTCTGA ATGGCTTCAC GCCTCTCCAC 
ATTGCCTGCA AGAAGAACCA CATTCGGGTG ATGGAGTTGC TTCTGAAGAC AGGAGCCTCC 
ATTGATGCTG TCACAGAGTC TGGCCTGACC CCCTTGCATG TGGCTGCCTT CATGGGGCAC 
TTGCCCATTG TCAAAACCTT GCTGCAGCGT GGAGCATCTC CTAATGTGTC CAATGTGAAA 
GTGGAGACTC CCCTGCACAT GGCAGCCAGA GCTGGGCACA TGGATGTGGC CAAGTATCTG 
ATACAGAACA AGGCCAAAAT CAACGCCAAG GCCAAGGATG ACCAGACTCC TCTGCACTGT 
GCTGCACGCA TTGGCCACAC CAGCATGGTC CAACTCCTGC TGGAGAACAA TGCCGACCCC 
AACCTGGCCA CTACAGCAGG GCACACGCCT CTGCACATCG CTGCCAGAGA GGGGCATGTA 
GACACAGCCT TGGCCCTGCT AGAGAAGGGA GCCTCTCAGA CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TCCACGTTGC AGCCAAGTAT GGGAAGGTGG ATGTGGCAGA GCTGCTGCTG 
GTGCATGATG CTCACCCCAA TGCAGCGGGG AAGAACGGCC TGACCCCACT GCACGTAGCT 
GTGCACCACA ACAACCTGGA GATCGTCAAG CTTCTGCTTC CCAAGGGGAG CTCTCCACAC 
AGCTCAGCCT GGAACGGGTA CACCCCTCTG CACATTGCCG CCAAGCAGAA CCAGATGGAG 
GTGGCCAGCA GCTTGCTGCA GTATGGTGCT TCTGCAAATG CGGAGTCTGT TCAGGGAGTC 
ACCCCTCTGC ACCTGGCTTC TCAGGAAGGG CATACAGACA TGGTAGCACT GCTTTTCTCC 
AAGCAAGCCA ATGGAAACTT AGGCAACAAG AGTGGTCTGA CTCCTCTCCA TCTCGTGGCC 
CAAGAGGGAC ACGTACCAGT GGCTGATGTT CTGGTGAAAC ATGGAGTCAC AGTGGATGCG 
ACAACCAGGA TGGGCTACAC CTCACTGCAC ATAGCCAGCC ACTATGGGAA CATCAAGTTG 
GTGAAGTTTC TGCTGCAGCA CCAGGCTGAT GTCAATGCTA AGACTAAGCT GGGCTACACA 
CCCCTGCACC AGGCGGCACA GCAGGGCCAC ACGGATGTGG TGACCCTGCT GCTGAAGCAT 
GGTGCCTCTC CCAACGAGAT CAGCACAAAT GGCACCACTC CCCTGGCCAT TGCGAAGCGC 
CTCGGTTACA TTTCCGTCAC AGATGTGCTC AAGATAGTCA CAGAGGAGAC CACCATCCCA 
CCATTCAGTG ACAAGTACCG CATGAGCTTC CCGGAGACTG TGGATGAGAT TCTGGATGTG 
TCGGAGGATG AAGGCACTGC TCATGTCACA GTAATGGAGG AGGAGCTGGT CCCACCGAAA 
CCCAGAACAC CTGAACCCAA GGAGCAGGAG GGCAAGAGGG AGATGCTGGA GTTTGTGACC 
GTGACAACAC TGGAGCAAAT GGAGGAATCT GCAGCTGTCC CACAGGTCCC CTGCGTGCCC 
CCTGAGACTG TGGTGACCAT AGCAGAGGAG TCTGAGCAGA TGGGACCTGT GGAGACTGAG 
GCTGAGCAAG GCGGCCTGCT GCCTGCACCC TCGGTGTCCC CACAGGAGCC CTCCAAGGAG 
TTCGATGAGG ACTCCTTGAT TCCCAGCAGC CCGGCCACCG AAACCTCGGA CAACATCAGC 
CCCGTGGCCA GCCCCGTTCA CACAGGGTTC CTGGTGAGCT TCATGGTGGA TGCGCGTGGC 
GGCTCCATGC GGGGCAGCCG GCACCACGGG CTGCGCGTGG TCATCCCTCC CCGTGCCTGT 
GCTGCACCCA CCCGCATCAC TTGCCGCCTG GTGAAGCCTC AGAAGCTGCC TGCCCCCCCA 
CCGCTGGCTG AGGAGGAGGG CCTGGCAAGC CGCATCATTG CACTGGGGCC CGCTGGCGCC 
CAGTTCCTCA GCCCCGTCAT TGTGGAGATT CCTCACTTTG CCTCCTATGG ACGTGGGGAC 
CGTGAGCTGG TGGTGTTGCG CAGTGAGAAT GGCTCTGTCT GGAAGGAGCA CCGCAACCGC 
TACGAGGAGA GCTACATGGA CCAACTGCTC AGTGGCATGG ATGAGGAGCT GGAGAGCCAA 
GAGGAGCTGG AGAAGAAGAG GGTCTGCCGC ATCATCACCA CTGACTTTCC TCTCTACTTC 
GTGGTCATGT CCCGGATTTG CCAGGACTGT GACATGATTG GCCCCGAGGG AGGGTGCTTG 
AAAAGCACAC TGGTGCCCAT GGTGCAGGCC ACCTTCCCAG ACACCGCTGT CACCAAGAAA 
GTGAGGCTGG CTCTGCAGGC GCAGCCCGTG CCTGATGAGC TGGTGACCAA GCTGCTGGGG 
AACCAGGCAA CCTTCAGCCC CATCGTCACA GTGGAGCCAC GTCGGAGGAA GTTCCACCGC 
CCCATTGGCC TCCGCATCCC TCTGCCCCCA TCCTGGAAGG ACAATCCCCG GGACAGCGGC 
GAGGGTGACA CCACCAGCCT GCGCCTGCTC TGTTCTGTGA TAGGAGGGAC AGCACAAGCT 
CAGTGGGAAG ACATCACAGG CACCACAAAG CTGGTCTATG AAAAAGAGTG TGCTAACTTT 
ACCACCAATG TGTCTGCCAG GTTCTGGCTG GCTGACTGCC CGCGCACTGC TGAGGCTGTG 
CACTTTGCCA CCGTGCTGTA CAAGGAACTG ACGGCTGTGC CCTACATGGC CAAATTTGTG 
GTGTTTGCTA AGATGAACGA TGGACGGGAA GGCCGGCTGC GCTGCTACTG CATGACTGAT 
GACAAGGTGG ACAAGACTTT GGAGCATCAT GAGAACTTCA CTGAGGTGGC CCGCAGCAGG 
GACATTGAGG TGGTGGAAGG AATGCCTCTG CATGTCGAGC TCTCAGGGAA CCTGGTGCCC 
GTCAAGAAGG CCACGCAGTC ACGCACCTTC TTCTTCCAGT CCTTCCGGGA AAGCCGCCTC 
GCCATCCCCA TCAAGGTCCG GGACAGCAGC CGGGAAGCCA GTGGCTCCCT GTCCTTCCTG 
CGCAAGGCCA TGAAGTATGA GGACTTGCAG CATGTGCTCT GCCACCTCAA CATCAGCATT 
CCACCCTGCA CCAAGGGAAG CAGCACTGAG GAGCGAAGGA GGACACTGAC ACCTTTGTCT 
CTGCGCTCTC TGAGCAGCAC CGACAGGGCA GACCAGAAGA TGATCGACAT TGCAGAGCAG 
CTTGGCCTCA GCTGGGCAGA GCTGGCACGT GAGCTGCAGT TTGGGGTGGA TGACATCAAC 
AGGATTCGTG TGGAGAACCC TAACTCCTTG CTGGAGCAGA GCATAGCCTT GCTTAACCTC 
TGGGTCAGCC GCGAGGGCAA GGGTGTCAAA ATTGAGAACC TGTACACAGC GCTGAGGAAC 
ATTGACCGCA GCGAGATCAT CAGCACGTTG GAGGGCTCCG GCCGGCAGAG CCGCAGCATG 
AAGGGCAGCT GGCGTTACAT GGACAGGGAC TATTCACTCT CGCCCTCCCA GATGAATGGT 
TACGCTTCGC TGCAGGACGA GCTGCTCTCC CCTGCCTCCC TGCATTATAT GCTGCCATCC 
CCTCTGCGTG CTGACCAGTA CTGGAACGAG GTGGCCATCA TGGACGCCAT CCCCATGGCC 
GCCACTGAGC AGGACGCCCT GATGGAGATG TCTGACATGC AGGTGTGGTC CTCAGGGCTC 
ACCCCTTCAC TGGTGACGGC TGAGGACTCC TCTCTGGAGT GCAGCAAGGC TGAGGACTCG 
GATGCTACAA GCGAAGGTCG TTTCCCAGGG CACCTTCTGA CAGATGCACA TGTCCCAGAC 
CACATGGGCT CTATGGACTT AGTTGAGGAT GACACAGTGG ACTCAGACGC CATGAACGGG 
CTGATTGAAC TGCTAGAGCA GGAGGAGGGG CAGAGGCCAG AGGGGAAGAT GCCAGCCAGC 
GACCACCAGC TAGGGACTGG AGAGCAGAAC CCAGAGAGTG AAGTCTCTTT TGTTTCAGTT 
CAACAGAAGG TACAAGCCAC GATCGTGCCA TCGCCAACCG TGAGCCACAC AGTGGAGAGG 
AGTGCAGACA GGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK1 ( XM_015297389.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152875.1 Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X1, mRNA.

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CloneID	OGa45385
Clone ID Related Accession (Same CDS sequence)	XM_015297389.1
Accession Version	XM_015297389.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ankyrin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455999.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGAGAG GGCATGCACC AACCACTGTG GACCCCTTGC CTTCTTGTTG CAAGCAAGGA 
TCTCCTGAAG GGATGCTGGA GCAGGAGTCT GCTGTGCAGG CTGATGCTGC GACCAGCTTC 
CTGAGAGCTG CAAGATCTGG GAATCTGGAC AGAGCCTTGG ATCATCTCAG GAATGGGGTA 
GATATTAACA CCTGTAACCA GAATGGGCTG AATGCCTTGC ACCTGGCCTC CAAGGAGGGC 
CACACGAAAA TGGTGGTGGA GCTTCTGCAC AAGGAGATTG TTTTGGAGAC CACTACCAAG 
AAGGGAAACA CAGCCCTGCA CATCGCTGCC CTCGCTGGAC AGCAGGACGT GGTCCGGGAA 
CTGGTGAACT ATGGGGCCAA TGTCAATGCT CAGTCGCAGA AGGGCTTCAC ACCACTCTAC 
ATGGCAGCGC AGGAAAACCA CCTGGAAGTT GTCAAATTCC TGCTGGAAAA CGGAGCCAAC 
CAGAATGTAG CCACAGAGGA TGGCTTCACA CCACTAGCTG TGGCTCTGCA GCAGGGTCAT 
GAGAATGTGG TTGCTCACCT TATCAACTAT GGGACGAAGG GTAAGGTCCG CCTGCCTGCC 
TTGCACATTG CAGCTCGCAA CGATGACACC CGTACAGCCG CCGTGCTGCT GCAGAATGAC 
CCCAATGCTG ACGTCCTCTC CAAGACTGGA TTTACACCTT TGCACATTGC AGCCCACTAT 
GAGAATCTCA GCGTGGCCCA ATTATTGCTG AATCGTGGAG CCAGCGTCAA CTTCACACCC 
CAGAATGGGA TCACTCCTTT GCACATAGCC TCTCGTCGAG GCAACATCAT CATGGTACGG 
CTGCTGCTGG ACCGTGGGGC CCAGATAGAG ACAAGGACCA AGGATGAGTT GACGCCTCTC 
CACTGTGCAG CTCGCAATGG ACATGTGCGA ATTGCAGAGA TCCTACTGGA CCATGGTGCT 
CCCATTCAAG CCAAAACAAA GAACGGTCTG TCACCGATCC ACATGGCAGC ACAGGGTGAC 
CACCTGGACT GTGTGCGCCT ACTCCTGCAG TACAGTGCTG AGATAGACGA CATCACCCTG 
GACCACCTGA CACCACTGCA TGTAGCTGCG CACTGTGGCC ACCACCGTGT GGCCAAGCTG 
CTGGTGGAGA AGGGAGCCAA GCCCAACTCC AGAGCTCTGA ATGGCTTCAC GCCTCTCCAC 
ATTGCCTGCA AGAAGAACCA CATTCGGGTG ATGGAGTTGC TTCTGAAGAC AGGAGCCTCC 
ATTGATGCTG TCACAGAGTC TGGCCTGACC CCCTTGCATG TGGCTGCCTT CATGGGGCAC 
TTGCCCATTG TCAAAACCTT GCTGCAGCGT GGAGCATCTC CTAATGTGTC CAATGTGAAA 
GTGGAGACTC CCCTGCACAT GGCAGCCAGA GCTGGGCACA TGGATGTGGC CAAGTATCTG 
ATACAGAACA AGGCCAAAAT CAACGCCAAG GCCAAGGATG ACCAGACTCC TCTGCACTGT 
GCTGCACGCA TTGGCCACAC CAGCATGGTC CAACTCCTGC TGGAGAACAA TGCCGACCCC 
AACCTGGCCA CTACAGCAGG GCACACGCCT CTGCACATCG CTGCCAGAGA GGGGCATGTA 
GACACAGCCT TGGCCCTGCT AGAGAAGGGA GCCTCTCAGA CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TCCACGTTGC AGCCAAGTAT GGGAAGGTGG ATGTGGCAGA GCTGCTGCTG 
GTGCATGATG CTCACCCCAA TGCAGCGGGG AAGAACGGCC TGACCCCACT GCACGTAGCT 
GTGCACCACA ACAACCTGGA GATCGTCAAG CTTCTGCTTC CCAAGGGGAG CTCTCCACAC 
AGCTCAGCCT GGAACGGGTA CACCCCTCTG CACATTGCCG CCAAGCAGAA CCAGATGGAG 
GTGGCCAGCA GCTTGCTGCA GTATGGTGCT TCTGCAAATG CGGAGTCTGT TCAGGGAGTC 
ACCCCTCTGC ACCTGGCTTC TCAGGAAGGG CATACAGACA TGGTAGCACT GCTTTTCTCC 
AAGCAAGCCA ATGGAAACTT AGGCAACAAG AGTGGTCTGA CTCCTCTCCA TCTCGTGGCC 
CAAGAGGGAC ACGTACCAGT GGCTGATGTT CTGGTGAAAC ATGGAGTCAC AGTGGATGCG 
ACAACCAGGA TGGGCTACAC CTCACTGCAC ATAGCCAGCC ACTATGGGAA CATCAAGTTG 
GTGAAGTTTC TGCTGCAGCA CCAGGCTGAT GTCAATGCTA AGACTAAGCT GGGCTACACA 
CCCCTGCACC AGGCGGCACA GCAGGGCCAC ACGGATGTGG TGACCCTGCT GCTGAAGCAT 
GGTGCCTCTC CCAACGAGAT CAGCACAAAT GGCACCACTC CCCTGGCCAT TGCGAAGCGC 
CTCGGTTACA TTTCCGTCAC AGATGTGCTC AAGATAGTCA CAGAGGAGAC CACCATCCCA 
CCATTCAGTG ACAAGTACCG CATGAGCTTC CCGGAGACCG TGGATGAGAT TCTGGATGTG 
TCGGAGGATG AAGGCACTGC TCATGTCACA GTAATGGAGG AGGAGCTGGT CCCACCGAAA 
CCCAGAACAC CTGAACCCAA GGAGCAGGAG GGCAAGAGGG AGATGCTGGA GTTTGTGACC 
GTGACAACAC TGGAGCAAAT GGAGGAATCT GCAGCTGTCC CACAGGTCCC CTGCGTGCCC 
CCTGAGACTG TGGTGACCAT AGCAGAGGAG TCTGAGCAGA TGGGACCTGT GGAGACTGAG 
GCTGAGCAAG GCGGCCTGCT GCCTGCACCC TCGGTGTCCC CACAGGAGCC CTCCAAGGAG 
TTCGATGAGG ACTCCTTGAT TCCCAGCAGC CCGGCCACCG AAACCTCGGA CAACATCAGC 
CCCGTGGCCA GCCCCGTTCA CACAGGGTTC CTGGTGAGCT TCATGGTGGA TGCGCGTGGC 
GGCTCCATGC GGGGCAGCCG GCACCACGGG CTGCGCGTGG TCATCCCTCC CCGTGCCTGT 
GCTGCACCCA CCCGCATCAC TTGCCGCCTG GTGAAGCCTC AGAAGCTGCC TGCCCCCCCA 
CCGCTGGCTG AGGAGGAGGG CCTGGCAAGC CGCATCATTG CACTGGGGCC CGCTGGCGCC 
CAGTTCCTCA GCCCCGTCAT TGTGGAGATT CCTCACTTTG CCTCCTATGG ACGTGGGGAC 
CGTGAGCTGG TGGTGTTGCG CAGTGAGAAT GGCTCTGTCT GGAAGGAGCA CCGCAACCGC 
TACGAGGAGA GCTACATGGA CCAACTGCTC AGTGGCATGG ATGAGGAGCT GGAGAGCCAA 
GAGGAGCTGG AGAAGAAGAG GGTCTGCCGC ATCATCACCA CTGACTTTCC TCTCTACTTC 
GTGGTCATGT CCCGGATTTG CCAGGACTGT GACATGATTG GCCCCGAGGG AGGGTGCTTG 
AAAAGCACAC TGGTGCCCAT GGTGCAGGCC ACCTTCCCAG ACACCGCTGT CACCAAGAAA 
GTGAGGCTGG CTCTGCAGGC GCAGCCCGTG CCTGATGAGC TGGTGACCAA GCTGCTGGGG 
AACCAGGCAA CCTTCAGCCC CATCGTCACA GTGGAGCCAC GTCGGAGGAA GTTCCACCGC 
CCCATTGGCC TCCGCATCCC TCTGCCCCCA TCCTGGAAGG ACAATCCCCG GGACAGCGGC 
GAGGGTGACA CCACCAGCCT GCGCCTGCTC TGTTCTGTGA TAGGAGGGAC AGCACAAGCT 
CAGTGGGAAG ACATCACAGG CACCACAAAG CTGGTCTATG AAAAAGAGTG TGCTAACTTT 
ACCACCAATG TGTCTGCCAG GTTCTGGCTG GCTGACTGCC CGCGCACTGC TGAGGCTGTG 
CACTTTGCCA CCGTGCTGTA CAAGGAACTG ACGGCTGTGC CCTACATGGC CAAATTTGTG 
GTGTTTGCTA AGATGAACGA TGGACGGGAA GGCCGGCTGC GCTGCTACTG CATGACTGAT 
GACAAGGTGG ACAAGACTTT GGAGCATCAT GAGAACTTCA CTGAGGTGGC CCGCAGCAGG 
GACATTGAGG TGGTGGAAGG AATGCCTCTG CATGTCGAGC TCTCAGGGAA CCTGGTGCCC 
GTCAAGAAGG CCACGCAGTC ACGCACCTTC TTCTTCCAGT CCTTCCGGGA AAGCCGCCTC 
GCCATCCCCA TCAAGGTCCG GGACAGCAGC CGGGAAGCCA GTGGCTCCCT GTCCTTCCTG 
CGCAAGGCCA TGAAGTATGA GGACTTGCAG CATGTGCTCT GCCACCTCAA CATCAGCATT 
CCACCCTGCA CCAAGGGAAG CAGCACTGAG GAGCGAAGGA GGACACTGAC ACCTTTGTCT 
CTGCGCTCTC TGAGCAGCAC CGACAGGGCA GACCAGAAGA TGATCGACAT TGCAGAGCAG 
CTTGGCCTCA GCTGGGCAGA GCTGGCACGT GAGCTGCAGT TTGGGGTGGA TGACATCAAC 
AGGATTCGTG TGGAGAACCC TAACTCCTTG CTGGAGCAGA GCATAGCCTT GCTTAACCTC 
TGGGTCAGCC GCGAGGGCAA GGGTGTCAAA ATTGAGAACC TGTACACAGC GCTGAGGAAC 
ATTGACCGCA GCGAGATCAT CAGCACGTTG GAGGGCTCCG GCCGGCAGAG CCGCAGCATG 
AAGGGCAGCT GGCGTTACAT GGACAGGGAC TATTCACTCT CGCCCTCCCA GATGAATGGT 
TACGCTTCGC TGCAGGACGA GCTGCTCTCC CCTGCCTCCC TGCATTATAT GCTGCCATCC 
CCTCTGCGTG CTGACCAGTA CTGGAACGAG GTGGCCATCA TGGACGCCAT CCCCATGGCC 
GCCACTGAGC AGGACGCCCT GATGGAGATG TCTGACATGC AGGTGTGGTC CTCAGGGCTC 
ACCCCTTCAC TGGTGACGGC TGAGGACTCC TCTCTGGAGT GCAGCAAGGC TGAGGACTCG 
GATGCTACAA GCGAAGGTCG TTTCCCAGGG CACCTTCTGA CAGATGCACA TGTCCCAGAC 
CACATGGGCT CTATGGACTT AGTTGAGGAT GACACAGTGG ACTCAGACGC CATGAACGGG 
CTGATTGAAC TGCTAGAGCA GGAGGAGGGG CAGAGGCCAG AGGGGAAGAT GCCAGCCAGC 
GACCACCAGC TAGGGACTGG AGAGCAGAAC CCAGAGAGTG AAGTCTCTTT TGTTTCAGTT 
CAACAGAAGG TACAAGCCAC GATCGTGCCA TCGCCAACCG TGAGCCACAC AGTGGAGAGG 
AGTGCAGACA GGCTGAGGGA CTGGAATGCA GAAGGCTCCT TTATCTCCTG CCTACAGGAC 
CTGACAGAGG GCTCCTGGCA GGAGGGGGTC ACCCGACGGC TGGGTCCAGC ACACACCGTG 
GCTGCTGGGG CGCAGGGCCA GGAGCAAGAG CAGGTCCTGG CGCCGGACGT GGAGCTGATG 
CGGGTCAGCT CCACAGAGGA CAGCGACTGG CAGCCCCAGC ACCCCGCGGG CGGCTGGCCA 
GAGGAGCCTG ACAGCCGCTG CTTTGGGCAG GGCAACGAAG TCCTCCATCT CCCTGGGGAG 
CATGTGACTG AGGAGCAGTT CACAGATGAA CAAGGCAATA TAATCACCAA GAAGGTCATC 
CGGAAAGTGG TGCATCAGTT GGACCTCGAC ACGGATGACA GGAAGGAGCA TGAGGACTTG 
ATTCTGGAGG GCTCCCTGCA GGAGCCCCAA GACCTGGAGG CAGAGGCTGA TCACTTCATG 
TACTCCATCC TACAGCGGGA CGGTCTGGGG GCCAAGGAGG AGGTGCAAGT GCGTGTCCCA 
AAACTGGAAG TCTCCGGGAG CAGGATGGGG GCTCAGATAG TGAAACGAGC CAGTCTGAAA 
AGGGGGAAGC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152875.1
CDS	874..6828
Translation	MAQAAKQLKKIKDIEAQALQEQKEKEESNRKRRNRSRDRKKKRGHAPTTVDPLPSCCKQGSPEGMLEQESAVQADAATSFLRAARSGNLDRALDHLRNGVDINTCNQNGLNALHLASKEGHTKMVVELLHKEIVLETTTKKGNTALHIAALAGQQDVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLSVAQLLLNRGASVNFTPQNGITPLHIASRRGNIIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRIAEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYSAEIDDITLDHLTPLHVAAHCGHHRVAKLLVEKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVAAFMGHLPIVKTLLQRGASPNVSNVKVETPLHMAARAGHMDVAKYLIQNKAKINAKAKDDQTPLHCAARIGHTSMVQLLLENNADPNLATTAGHTPLHIAAREGHVDTALALLEKGASQTCMTKKGFTPLHVAAKYGKVDVAELLLVHDAHPNAAGKNGLTPLHVAVHHNNLEIVKLLLPKGSSPHSSAWNGYTPLHIAAKQNQMEVASSLLQYGASANAESVQGVTPLHLASQEGHTDMVALLFSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLVKHGVTVDATTRMGYTSLHIASHYGNIKLVKFLLQHQADVNAKTKLGYTPLHQAAQQGHTDVVTLLLKHGASPNEISTNGTTPLAIAKRLGYISVTDVLKIVTEETTIPPFSDKYRMSFPETVDEILDVSEDEGTAHVTVMEEELVPPKPRTPEPKEQEGKREMLEFVTVTTLEQMEESAAVPQVPCVPPETVVTIAEESEQMGPVETEAEQGGLLPAPSVSPQEPSKEFDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHHGLRVVIPPRACAAPTRITCRLVKPQKLPAPPPLAEEEGLASRIIALGPAGAQFLSPVIVEIPHFASYGRGDRELVVLRSENGSVWKEHRNRYEESYMDQLLSGMDEELESQEELEKKRVCRIITTDFPLYFVVMSRICQDCDMIGPEGGCLKSTLVPMVQATFPDTAVTKKVRLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWKDNPRDSGEGDTTSLRLLCSVIGGTAQAQWEDITGTTKLVYEKECANFTTNVSARFWLADCPRTAEAVHFATVLYKELTAVPYMAKFVVFAKMNDGREGRLRCYCMTDDKVDKTLEHHENFTEVARSRDIEVVEGMPLHVELSGNLVPVKKATQSRTFFFQSFRESRLAIPIKVRDSSREASGSLSFLRKAMKYEDLQHVLCHLNISIPPCTKGSSTEERRRTLTPLSLRSLSSTDRADQKMIDIAEQLGLSWAELARELQFGVDDINRIRVENPNSLLEQSIALLNLWVSREGKGVKIENLYTALRNIDRSEIISTLEGSGRQSRSMKGSWRYMDRDYSLSPSQMNGYASLQDELLSPASLHYMLPSPLRADQYWNEVAIMDAIPMAATEQDALMEMSDMQVWSSGLTPSLVTAEDSSLECSKAEDSDATSEGRFPGHLLTDAHVPDHMGSMDLVEDDTVDSDAMNGLIELLEQEEGQRPEGKMPASDHQLGTGEQNPESEVSFVSVQQKVQATIVPSPTVSHTVERSADRLRDWNAEGSFISCLQDLTEGSWQEGVTRRLGPAHTVAAGAQGQEQEQVLAPDVELMRVSSTEDSDWQPQHPAGGWPEEPDSRCFGQGNEVLHLPGEHVTEEQFTDEQGNIITKKVIRKVVHQLDLDTDDRKEHEDLILEGSLQEPQDLEAEADHFMYSILQRDGLGAKEEVQVRVPKLEVSGSRMGAQIVKRASLKRGKH

Target ORF information:

RefSeq Version	XM_015297389.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297389.1

ORF Insert Sequence:

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGAGAG GGCATGCACC AACCACTGTG GACCCCTTGC CTTCTTGTTG CAAGCAAGGA 
TCTCCTGAAG GGATGCTGGA GCAGGAGTCT GCTGTGCAGG CTGATGCTGC GACCAGCTTC 
CTGAGAGCTG CAAGATCTGG GAATCTGGAC AGAGCCTTGG ATCATCTCAG GAATGGGGTA 
GATATTAACA CCTGTAACCA GAATGGGCTG AATGCCTTGC ACCTGGCCTC CAAGGAGGGC 
CACACGAAAA TGGTGGTGGA GCTTCTGCAC AAGGAGATTG TTTTGGAGAC CACTACCAAG 
AAGGGAAACA CAGCCCTGCA CATCGCTGCC CTCGCTGGAC AGCAGGACGT GGTCCGGGAA 
CTGGTGAACT ATGGGGCCAA TGTCAATGCT CAGTCGCAGA AGGGCTTCAC ACCACTCTAC 
ATGGCAGCGC AGGAAAACCA CCTGGAAGTT GTCAAATTCC TGCTGGAAAA CGGAGCCAAC 
CAGAATGTAG CCACAGAGGA TGGCTTCACA CCACTAGCTG TGGCTCTGCA GCAGGGTCAT 
GAGAATGTGG TTGCTCACCT TATCAACTAT GGGACGAAGG GTAAGGTCCG CCTGCCTGCC 
TTGCACATTG CAGCTCGCAA CGATGACACC CGTACAGCCG CCGTGCTGCT GCAGAATGAC 
CCCAATGCTG ACGTCCTCTC CAAGACTGGA TTTACACCTT TGCACATTGC AGCCCACTAT 
GAGAATCTCA GCGTGGCCCA ATTATTGCTG AATCGTGGAG CCAGCGTCAA CTTCACACCC 
CAGAATGGGA TCACTCCTTT GCACATAGCC TCTCGTCGAG GCAACATCAT CATGGTACGG 
CTGCTGCTGG ACCGTGGGGC CCAGATAGAG ACAAGGACCA AGGATGAGTT GACGCCTCTC 
CACTGTGCAG CTCGCAATGG ACATGTGCGA ATTGCAGAGA TCCTACTGGA CCATGGTGCT 
CCCATTCAAG CCAAAACAAA GAACGGTCTG TCACCGATCC ACATGGCAGC ACAGGGTGAC 
CACCTGGACT GTGTGCGCCT ACTCCTGCAG TACAGTGCTG AGATAGACGA CATCACCCTG 
GACCACCTGA CACCACTGCA TGTAGCTGCG CACTGTGGCC ACCACCGTGT GGCCAAGCTG 
CTGGTGGAGA AGGGAGCCAA GCCCAACTCC AGAGCTCTGA ATGGCTTCAC GCCTCTCCAC 
ATTGCCTGCA AGAAGAACCA CATTCGGGTG ATGGAGTTGC TTCTGAAGAC AGGAGCCTCC 
ATTGATGCTG TCACAGAGTC TGGCCTGACC CCCTTGCATG TGGCTGCCTT CATGGGGCAC 
TTGCCCATTG TCAAAACCTT GCTGCAGCGT GGAGCATCTC CTAATGTGTC CAATGTGAAA 
GTGGAGACTC CCCTGCACAT GGCAGCCAGA GCTGGGCACA TGGATGTGGC CAAGTATCTG 
ATACAGAACA AGGCCAAAAT CAACGCCAAG GCCAAGGATG ACCAGACTCC TCTGCACTGT 
GCTGCACGCA TTGGCCACAC CAGCATGGTC CAACTCCTGC TGGAGAACAA TGCCGACCCC 
AACCTGGCCA CTACAGCAGG GCACACGCCT CTGCACATCG CTGCCAGAGA GGGGCATGTA 
GACACAGCCT TGGCCCTGCT AGAGAAGGGA GCCTCTCAGA CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TCCACGTTGC AGCCAAGTAT GGGAAGGTGG ATGTGGCAGA GCTGCTGCTG 
GTGCATGATG CTCACCCCAA TGCAGCGGGG AAGAACGGCC TGACCCCACT GCACGTAGCT 
GTGCACCACA ACAACCTGGA GATCGTCAAG CTTCTGCTTC CCAAGGGGAG CTCTCCACAC 
AGCTCAGCCT GGAACGGGTA CACCCCTCTG CACATTGCCG CCAAGCAGAA CCAGATGGAG 
GTGGCCAGCA GCTTGCTGCA GTATGGTGCT TCTGCAAATG CGGAGTCTGT TCAGGGAGTC 
ACCCCTCTGC ACCTGGCTTC TCAGGAAGGG CATACAGACA TGGTAGCACT GCTTTTCTCC 
AAGCAAGCCA ATGGAAACTT AGGCAACAAG AGTGGTCTGA CTCCTCTCCA TCTCGTGGCC 
CAAGAGGGAC ACGTACCAGT GGCTGATGTT CTGGTGAAAC ATGGAGTCAC AGTGGATGCG 
ACAACCAGGA TGGGCTACAC CTCACTGCAC ATAGCCAGCC ACTATGGGAA CATCAAGTTG 
GTGAAGTTTC TGCTGCAGCA CCAGGCTGAT GTCAATGCTA AGACTAAGCT GGGCTACACA 
CCCCTGCACC AGGCGGCACA GCAGGGCCAC ACGGATGTGG TGACCCTGCT GCTGAAGCAT 
GGTGCCTCTC CCAACGAGAT CAGCACAAAT GGCACCACTC CCCTGGCCAT TGCGAAGCGC 
CTCGGTTACA TTTCCGTCAC AGATGTGCTC AAGATAGTCA CAGAGGAGAC CACCATCCCA 
CCATTCAGTG ACAAGTACCG CATGAGCTTC CCGGAGACCG TGGATGAGAT TCTGGATGTG 
TCGGAGGATG AAGGCACTGC TCATGTCACA GTAATGGAGG AGGAGCTGGT CCCACCGAAA 
CCCAGAACAC CTGAACCCAA GGAGCAGGAG GGCAAGAGGG AGATGCTGGA GTTTGTGACC 
GTGACAACAC TGGAGCAAAT GGAGGAATCT GCAGCTGTCC CACAGGTCCC CTGCGTGCCC 
CCTGAGACTG TGGTGACCAT AGCAGAGGAG TCTGAGCAGA TGGGACCTGT GGAGACTGAG 
GCTGAGCAAG GCGGCCTGCT GCCTGCACCC TCGGTGTCCC CACAGGAGCC CTCCAAGGAG 
TTCGATGAGG ACTCCTTGAT TCCCAGCAGC CCGGCCACCG AAACCTCGGA CAACATCAGC 
CCCGTGGCCA GCCCCGTTCA CACAGGGTTC CTGGTGAGCT TCATGGTGGA TGCGCGTGGC 
GGCTCCATGC GGGGCAGCCG GCACCACGGG CTGCGCGTGG TCATCCCTCC CCGTGCCTGT 
GCTGCACCCA CCCGCATCAC TTGCCGCCTG GTGAAGCCTC AGAAGCTGCC TGCCCCCCCA 
CCGCTGGCTG AGGAGGAGGG CCTGGCAAGC CGCATCATTG CACTGGGGCC CGCTGGCGCC 
CAGTTCCTCA GCCCCGTCAT TGTGGAGATT CCTCACTTTG CCTCCTATGG ACGTGGGGAC 
CGTGAGCTGG TGGTGTTGCG CAGTGAGAAT GGCTCTGTCT GGAAGGAGCA CCGCAACCGC 
TACGAGGAGA GCTACATGGA CCAACTGCTC AGTGGCATGG ATGAGGAGCT GGAGAGCCAA 
GAGGAGCTGG AGAAGAAGAG GGTCTGCCGC ATCATCACCA CTGACTTTCC TCTCTACTTC 
GTGGTCATGT CCCGGATTTG CCAGGACTGT GACATGATTG GCCCCGAGGG AGGGTGCTTG 
AAAAGCACAC TGGTGCCCAT GGTGCAGGCC ACCTTCCCAG ACACCGCTGT CACCAAGAAA 
GTGAGGCTGG CTCTGCAGGC GCAGCCCGTG CCTGATGAGC TGGTGACCAA GCTGCTGGGG 
AACCAGGCAA CCTTCAGCCC CATCGTCACA GTGGAGCCAC GTCGGAGGAA GTTCCACCGC 
CCCATTGGCC TCCGCATCCC TCTGCCCCCA TCCTGGAAGG ACAATCCCCG GGACAGCGGC 
GAGGGTGACA CCACCAGCCT GCGCCTGCTC TGTTCTGTGA TAGGAGGGAC AGCACAAGCT 
CAGTGGGAAG ACATCACAGG CACCACAAAG CTGGTCTATG AAAAAGAGTG TGCTAACTTT 
ACCACCAATG TGTCTGCCAG GTTCTGGCTG GCTGACTGCC CGCGCACTGC TGAGGCTGTG 
CACTTTGCCA CCGTGCTGTA CAAGGAACTG ACGGCTGTGC CCTACATGGC CAAATTTGTG 
GTGTTTGCTA AGATGAACGA TGGACGGGAA GGCCGGCTGC GCTGCTACTG CATGACTGAT 
GACAAGGTGG ACAAGACTTT GGAGCATCAT GAGAACTTCA CTGAGGTGGC CCGCAGCAGG 
GACATTGAGG TGGTGGAAGG AATGCCTCTG CATGTCGAGC TCTCAGGGAA CCTGGTGCCC 
GTCAAGAAGG CCACGCAGTC ACGCACCTTC TTCTTCCAGT CCTTCCGGGA AAGCCGCCTC 
GCCATCCCCA TCAAGGTCCG GGACAGCAGC CGGGAAGCCA GTGGCTCCCT GTCCTTCCTG 
CGCAAGGCCA TGAAGTATGA GGACTTGCAG CATGTGCTCT GCCACCTCAA CATCAGCATT 
CCACCCTGCA CCAAGGGAAG CAGCACTGAG GAGCGAAGGA GGACACTGAC ACCTTTGTCT 
CTGCGCTCTC TGAGCAGCAC CGACAGGGCA GACCAGAAGA TGATCGACAT TGCAGAGCAG 
CTTGGCCTCA GCTGGGCAGA GCTGGCACGT GAGCTGCAGT TTGGGGTGGA TGACATCAAC 
AGGATTCGTG TGGAGAACCC TAACTCCTTG CTGGAGCAGA GCATAGCCTT GCTTAACCTC 
TGGGTCAGCC GCGAGGGCAA GGGTGTCAAA ATTGAGAACC TGTACACAGC GCTGAGGAAC 
ATTGACCGCA GCGAGATCAT CAGCACGTTG GAGGGCTCCG GCCGGCAGAG CCGCAGCATG 
AAGGGCAGCT GGCGTTACAT GGACAGGGAC TATTCACTCT CGCCCTCCCA GATGAATGGT 
TACGCTTCGC TGCAGGACGA GCTGCTCTCC CCTGCCTCCC TGCATTATAT GCTGCCATCC 
CCTCTGCGTG CTGACCAGTA CTGGAACGAG GTGGCCATCA TGGACGCCAT CCCCATGGCC 
GCCACTGAGC AGGACGCCCT GATGGAGATG TCTGACATGC AGGTGTGGTC CTCAGGGCTC 
ACCCCTTCAC TGGTGACGGC TGAGGACTCC TCTCTGGAGT GCAGCAAGGC TGAGGACTCG 
GATGCTACAA GCGAAGGTCG TTTCCCAGGG CACCTTCTGA CAGATGCACA TGTCCCAGAC 
CACATGGGCT CTATGGACTT AGTTGAGGAT GACACAGTGG ACTCAGACGC CATGAACGGG 
CTGATTGAAC TGCTAGAGCA GGAGGAGGGG CAGAGGCCAG AGGGGAAGAT GCCAGCCAGC 
GACCACCAGC TAGGGACTGG AGAGCAGAAC CCAGAGAGTG AAGTCTCTTT TGTTTCAGTT 
CAACAGAAGG TACAAGCCAC GATCGTGCCA TCGCCAACCG TGAGCCACAC AGTGGAGAGG 
AGTGCAGACA GGCTGAGGGA CTGGAATGCA GAAGGCTCCT TTATCTCCTG CCTACAGGAC 
CTGACAGAGG GCTCCTGGCA GGAGGGGGTC ACCCGACGGC TGGGTCCAGC ACACACCGTG 
GCTGCTGGGG CGCAGGGCCA GGAGCAAGAG CAGGTCCTGG CGCCGGACGT GGAGCTGATG 
CGGGTCAGCT CCACAGAGGA CAGCGACTGG CAGCCCCAGC ACCCCGCGGG CGGCTGGCCA 
GAGGAGCCTG ACAGCCGCTG CTTTGGGCAG GGCAACGAAG TCCTCCATCT CCCTGGGGAG 
CATGTGACTG AGGAGCAGTT CACAGATGAA CAAGGCAATA TAATCACCAA GAAGGTCATC 
CGGAAAGTGG TGCATCAGTT GGACCTCGAC ACGGATGACA GGAAGGAGCA TGAGGACTTG 
ATTCTGGAGG GCTCCCTGCA GGAGCCCCAA GACCTGGAGG CAGAGGCTGA TCACTTCATG 
TACTCCATCC TACAGCGGGA CGGTCTGGGG GCCAAGGAGG AGGTGCAAGT GCGTGTCCCA 
AAACTGGAAG TCTCCGGGAG CAGGATGGGG GCTCAGATAG TGAAACGAGC CAGTCTGAAA 
AGGGGGAAGC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK1 ( XM_015297390.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152876.1 Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X2, mRNA.

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CloneID	OGa45386
Clone ID Related Accession (Same CDS sequence)	XM_015297390.1
Accession Version	XM_015297390.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ankyrin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455999.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGAGAG GGCATGCACC AACCACTGTG GACCCCTTGC CTTCTTGTTG CAAGCAAGGA 
TCTCCTGAAG GGATGCTGGA GCAGGAGTCT GCTGTGCAGG CTGATGCTGC GACCAGCTTC 
CTGAGAGCTG CAAGATCTGG GAATCTGGAC AGAGCCTTGG ATCATCTCAG GAATGGGGTA 
GATATTAACA CCTGTAACCA GAATGGGCTG AATGCCTTGC ACCTGGCCTC CAAGGAGGGC 
CACACGAAAA TGGTGGTGGA GCTTCTGCAC AAGGAGATTG TTTTGGAGAC CACTACCAAG 
AAGGGAAACA CAGCCCTGCA CATCGCTGCC CTCGCTGGAC AGCAGGACGT GGTCCGGGAA 
CTGGTGAACT ATGGGGCCAA TGTCAATGCT CAGTCGCAGA AGGGCTTCAC ACCACTCTAC 
ATGGCAGCGC AGGAAAACCA CCTGGAAGTT GTCAAATTCC TGCTGGAAAA CGGAGCCAAC 
CAGAATGTAG CCACAGAGGA TGGCTTCACA CCACTAGCTG TGGCTCTGCA GCAGGGTCAT 
GAGAATGTGG TTGCTCACCT TATCAACTAT GGGACGAAGG GTAAGGTCCG CCTGCCTGCC 
TTGCACATTG CAGCTCGCAA CGATGACACC CGTACAGCCG CCGTGCTGCT GCAGAATGAC 
CCCAATGCTG ACGTCCTCTC CAAGACTGGA TTTACACCTT TGCACATTGC AGCCCACTAT 
GAGAATCTCA GCGTGGCCCA ATTATTGCTG AATCGTGGAG CCAGCGTCAA CTTCACACCC 
CAGAATGGGA TCACTCCTTT GCACATAGCC TCTCGTCGAG GCAACATCAT CATGGTACGG 
CTGCTGCTGG ACCGTGGGGC CCAGATAGAG ACAAGGACCA AGGATGAGTT GACGCCTCTC 
CACTGTGCAG CTCGCAATGG ACATGTGCGA ATTGCAGAGA TCCTACTGGA CCATGGTGCT 
CCCATTCAAG CCAAAACAAA GAACGGTCTG TCACCGATCC ACATGGCAGC ACAGGGTGAC 
CACCTGGACT GTGTGCGCCT ACTCCTGCAG TACAGTGCTG AGATAGACGA CATCACCCTG 
GACCACCTGA CACCACTGCA TGTAGCTGCG CACTGTGGCC ACCACCGTGT GGCCAAGCTG 
CTGGTGGAGA AGGGAGCCAA GCCCAACTCC AGAGCTCTGA ATGGCTTCAC GCCTCTCCAC 
ATTGCCTGCA AGAAGAACCA CATTCGGGTG ATGGAGTTGC TTCTGAAGAC AGGAGCCTCC 
ATTGATGCTG TCACAGAGTC TGGCCTGACC CCCTTGCATG TGGCTGCCTT CATGGGGCAC 
TTGCCCATTG TCAAAACCTT GCTGCAGCGT GGAGCATCTC CTAATGTGTC CAATGTGAAA 
GTGGAGACTC CCCTGCACAT GGCAGCCAGA GCTGGGCACA TGGATGTGGC CAAGTATCTG 
ATACAGAACA AGGCCAAAAT CAACGCCAAG GCCAAGGATG ACCAGACTCC TCTGCACTGT 
GCTGCACGCA TTGGCCACAC CAGCATGGTC CAACTCCTGC TGGAGAACAA TGCCGACCCC 
AACCTGGCCA CTACAGCAGG GCACACGCCT CTGCACATCG CTGCCAGAGA GGGGCATGTA 
GACACAGCCT TGGCCCTGCT AGAGAAGGGA GCCTCTCAGA CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TCCACGTTGC AGCCAAGTAT GGGAAGGTGG ATGTGGCAGA GCTGCTGCTG 
GTGCATGATG CTCACCCCAA TGCAGCGGGG AAGAACGGCC TGACCCCACT GCACGTAGCT 
GTGCACCACA ACAACCTGGA GATCGTCAAG CTTCTGCTTC CCAAGGGGAG CTCTCCACAC 
AGCTCAGCCT GGAACGGGTA CACCCCTCTG CACATTGCCG CCAAGCAGAA CCAGATGGAG 
GTGGCCAGCA GCTTGCTGCA GTATGGTGCT TCTGCAAATG CGGAGTCTGT TCAGGGAGTC 
ACCCCTCTGC ACCTGGCTTC TCAGGAAGGG CATACAGACA TGGTAGCACT GCTTTTCTCC 
AAGCAAGCCA ATGGAAACTT AGGCAACAAG AGTGGTCTGA CTCCTCTCCA TCTCGTGGCC 
CAAGAGGGAC ACGTACCAGT GGCTGATGTT CTGGTGAAAC ATGGAGTCAC AGTGGATGCG 
ACAACCAGGA TGGGCTACAC CTCACTGCAC ATAGCCAGCC ACTATGGGAA CATCAAGTTG 
GTGAAGTTTC TGCTGCAGCA CCAGGCTGAT GTCAATGCTA AGACTAAGCT GGGCTACACA 
CCCCTGCACC AGGCGGCACA GCAGGGCCAC ACGGATGTGG TGACCCTGCT GCTGAAGCAT 
GGTGCCTCTC CCAACGAGAT CAGCACAAAT GGCACCACTC CCCTGGCCAT TGCGAAGCGC 
CTCGGTTACA TTTCCGTCAC AGATGTGCTC AAGATAGTCA CAGAGGAGAC CACCATCCCA 
CCATTCAGTG ACAAGTACCG CATGAGCTTC CCGGAGACCG TGGATGAGAT TCTGGATGTG 
TCGGAGGATG AAGGCACTGC TCATGTCACA GTAATGGAGG AGGAGCTGGT CCCACCGAAA 
CCCAGAACAC CTGAACCCAA GGAGCAGGAG GGCAAGAGGG AGATGCTGGA GTTTGTGACC 
GTGACAACAC TGGAGCAAAT GGAGGAATCT GCAGCTGTCC CACAGGTCCC CTGCGTGCCC 
CCTGAGACTG TGGTGACCAT AGCAGAGGAG TCTGAGCAGA TGGGACCTGT GGAGACTGAG 
GCTGAGCAAG GCGGCCTGCT GCCTGCACCC TCGGTGTCCC CACAGGAGCC CTCCAAGGAG 
TTCGATGAGG ACTCCTTGAT TCCCAGCAGC CCGGCCACCG AAACCTCGGA CAACATCAGC 
CCCGTGGCCA GCCCCGTTCA CACAGGGTTC CTGGTGAGCT TCATGGTGGA TGCGCGTGGC 
GGCTCCATGC GGGGCAGCCG GCACCACGGG CTGCGCGTGG TCATCCCTCC CCGTGCCTGT 
GCTGCACCCA CCCGCATCAC TTGCCGCCTG GTGAAGCCTC AGAAGCTGCC TGCCCCCCCA 
CCGCTGGCTG AGGAGGAGGG CCTGGCAAGC CGCATCATTG CACTGGGGCC CGCTGGCGCC 
CAGTTCCTCA GCCCCGTCAT TGTGGAGATT CCTCACTTTG CCTCCTATGG ACGTGGGGAC 
CGTGAGCTGG TGGTGTTGCG CAGTGAGAAT GGCTCTGTCT GGAAGGAGCA CCGCAACCGC 
TACGAGGAGA GCTACATGGA CCAACTGCTC AGTGGCATGG ATGAGGAGCT GGAGAGCCAA 
GAGGAGCTGG AGAAGAAGAG GGTCTGCCGC ATCATCACCA CTGACTTTCC TCTCTACTTC 
GTGGTCATGT CCCGGATTTG CCAGGACTGT GACATGATTG GCCCCGAGGG AGGGTGCTTG 
AAAAGCACAC TGGTGCCCAT GGTGCAGGCC ACCTTCCCAG ACACCGCTGT CACCAAGAAA 
GTGAGGCTGG CTCTGCAGGC GCAGCCCGTG CCTGATGAGC TGGTGACCAA GCTGCTGGGG 
AACCAGGCAA CCTTCAGCCC CATCGTCACA GTGGAGCCAC GTCGGAGGAA GTTCCACCGC 
CCCATTGGCC TCCGCATCCC TCTGCCCCCA TCCTGGAAGG ACAATCCCCG GGACAGCGGC 
GAGGGTGACA CCACCAGCCT GCGCCTGCTC TGTTCTGTGA TAGGAGGGAC AGCACAAGCT 
CAGTGGGAAG ACATCACAGG CACCACAAAG CTGGTCTATG AAAAAGAGTG TGCTAACTTT 
ACCACCAATG TGTCTGCCAG GTTCTGGCTG GCTGACTGCC CGCGCACTGC TGAGGCTGTG 
CACTTTGCCA CCGTGCTGTA CAAGGAACTG ACGGCTGTGC CCTACATGGC CAAATTTGTG 
GTGTTTGCTA AGATGAACGA TGGACGGGAA GGCCGGCTGC GCTGCTACTG CATGACTGAT 
GACAAGGTGG ACAAGACTTT GGAGCATCAT GAGAACTTCA CTGAGGTGGC CCGCAGCAGG 
GACATTGAGG TGGTGGAAGG AATGCCTCTG CATGTCGAGC TCTCAGGGAA CCTGGTGCCC 
GTCAAGAAGG CCACGCAGTC ACGCACCTTC TTCTTCCAGT CCTTCCGGGA AAGCCGCCTC 
GCCATCCCCA TCAAGGTCCG GGACAGCAGC CGGGAAGCCA GTGGCTCCCT GTCCTTCCTG 
CGCAAGGCCA TGAAGTATGA GGACTTGCAG CATGTGCTCT GCCACCTCAA CATCAGCATT 
CCACCCTGCA CCAAGGGAAG CAGCACTGAG GAGCGAAGGA GGACACTGAC ACCTTTGTCT 
CTGCGCTCTC TGAGCAGCAC CGACAGGGCA GACCAGAAGA TGATCGACAT TGCAGAGCAG 
CTTGGCCTCA GCTGGGCAGA GCTGGCACGT GAGCTGCAGT TTGGGGTGGA TGACATCAAC 
AGGATTCGTG TGGAGAACCC TAACTCCTTG CTGGAGCAGA GCATAGCCTT GCTTAACCTC 
TGGGTCAGCC GCGAGGGCAA GGGTGTCAAA ATTGAGAACC TGTACACAGC GCTGAGGAAC 
ATTGACCGCA GCGAGATCAT CAGCACGTTG GAGGGCTCCG GCCGGCAGAG CCGCAGCATG 
AAGGGCAGCT GGCGTTACAT GGACAGGGAC TATTCACTCT CGCCCTCCCA GATGAATGGT 
TACGCTTCGC TGCAGGACGA GCTGCTCTCC CCTGCCTCCC TGCATTATAT GCTGCCATCC 
CCTCTGCGTG CTGACCAGTA CTGGAACGAG GTGGCCATCA TGGACGCCAT CCCCATGGCC 
GCCACTGAGC AGGACGCCCT GATGGAGATG TCTGACATGC AGGTGTGGTC CTCAGGGCTC 
ACCCCTTCAC TGGTGACGGC TGAGGACTCC TCTCTGGAGT GCAGCAAGGC TGAGGACTCG 
GATGCTACAA GCGAAGGTCG TTTCCCAGGG CACCTTCTGA CAGATGCACA TGTCCCAGAC 
CACATGGGCT CTATGGACTT AGTTGAGGAT GACACAGTGG ACTCAGACGC CATGAACGGG 
CTGATTGAAC TGCTAGAGCA GGAGGAGGGG CAGAGGCCAG AGGGGAAGAT GCCAGCCAGC 
GACCACCAGC TAGGGACTGG AGAGCAGAAC CCAGAGAGTG AAGTCTCTTT TGTTTCAGTT 
CAACAGAAGG TACAAGCCAC GATCGTGCCA TCGCCAACCG TGAGCCACAC AGTGGAGAGG 
AGTGCAGACA GGCTGAGGGA CTGGAATGCA GAAGGCTCCT TTATCTCCTG CCTACAGGAC 
CTGACAGAGG GCTCCTGGCA GGAGGGGGTC ACCCGACGGC TGGGTCCAGC ACACACCGTG 
GCTGCTGGGG CGCAGGGCCA GGAGCAAGAG CAGGTCCTGG CGCCGGACGT GGAGCTGATG 
CGGGTCAGCT CCACAGAGGA CAGCGACTGG CAGCCCCAGC ACCCCGCGGG CGGCTGGCCA 
GAGGAGCCTG ACAGCCGCTG CTTTGGGCAG GGCAACGAAG TCCTCCATCT CCCTGGGGAG 
CATGTGACTG AGGAGCAGTT CACAGATGAA CAAGGCAATA TAATCACCAA GAAGGTCATC 
CGGAAAGTGG TGCATCAGTT GGACCTCGAC ACGGATGACA GGAAGGAGCA TGAGGACTTG 
ATTCTGGAGG GCTCCCTGCA GGAGCCCCAA GACCTGGAGG CAGAGGCTGA TCACTTCATG 
TACTCCATCC TACAGCGGGA CGGTCTGGGG GCCAAGGATC TCACCTCAAC ACCAAACCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152876.1
CDS	871..6753
Translation	MAQAAKQLKKIKDIEAQALQEQKEKEESNRKRRNRSRDRKKKRGHAPTTVDPLPSCCKQGSPEGMLEQESAVQADAATSFLRAARSGNLDRALDHLRNGVDINTCNQNGLNALHLASKEGHTKMVVELLHKEIVLETTTKKGNTALHIAALAGQQDVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLSVAQLLLNRGASVNFTPQNGITPLHIASRRGNIIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRIAEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYSAEIDDITLDHLTPLHVAAHCGHHRVAKLLVEKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVAAFMGHLPIVKTLLQRGASPNVSNVKVETPLHMAARAGHMDVAKYLIQNKAKINAKAKDDQTPLHCAARIGHTSMVQLLLENNADPNLATTAGHTPLHIAAREGHVDTALALLEKGASQTCMTKKGFTPLHVAAKYGKVDVAELLLVHDAHPNAAGKNGLTPLHVAVHHNNLEIVKLLLPKGSSPHSSAWNGYTPLHIAAKQNQMEVASSLLQYGASANAESVQGVTPLHLASQEGHTDMVALLFSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLVKHGVTVDATTRMGYTSLHIASHYGNIKLVKFLLQHQADVNAKTKLGYTPLHQAAQQGHTDVVTLLLKHGASPNEISTNGTTPLAIAKRLGYISVTDVLKIVTEETTIPPFSDKYRMSFPETVDEILDVSEDEGTAHVTVMEEELVPPKPRTPEPKEQEGKREMLEFVTVTTLEQMEESAAVPQVPCVPPETVVTIAEESEQMGPVETEAEQGGLLPAPSVSPQEPSKEFDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHHGLRVVIPPRACAAPTRITCRLVKPQKLPAPPPLAEEEGLASRIIALGPAGAQFLSPVIVEIPHFASYGRGDRELVVLRSENGSVWKEHRNRYEESYMDQLLSGMDEELESQEELEKKRVCRIITTDFPLYFVVMSRICQDCDMIGPEGGCLKSTLVPMVQATFPDTAVTKKVRLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWKDNPRDSGEGDTTSLRLLCSVIGGTAQAQWEDITGTTKLVYEKECANFTTNVSARFWLADCPRTAEAVHFATVLYKELTAVPYMAKFVVFAKMNDGREGRLRCYCMTDDKVDKTLEHHENFTEVARSRDIEVVEGMPLHVELSGNLVPVKKATQSRTFFFQSFRESRLAIPIKVRDSSREASGSLSFLRKAMKYEDLQHVLCHLNISIPPCTKGSSTEERRRTLTPLSLRSLSSTDRADQKMIDIAEQLGLSWAELARELQFGVDDINRIRVENPNSLLEQSIALLNLWVSREGKGVKIENLYTALRNIDRSEIISTLEGSGRQSRSMKGSWRYMDRDYSLSPSQMNGYASLQDELLSPASLHYMLPSPLRADQYWNEVAIMDAIPMAATEQDALMEMSDMQVWSSGLTPSLVTAEDSSLECSKAEDSDATSEGRFPGHLLTDAHVPDHMGSMDLVEDDTVDSDAMNGLIELLEQEEGQRPEGKMPASDHQLGTGEQNPESEVSFVSVQQKVQATIVPSPTVSHTVERSADRLRDWNAEGSFISCLQDLTEGSWQEGVTRRLGPAHTVAAGAQGQEQEQVLAPDVELMRVSSTEDSDWQPQHPAGGWPEEPDSRCFGQGNEVLHLPGEHVTEEQFTDEQGNIITKKVIRKVVHQLDLDTDDRKEHEDLILEGSLQEPQDLEAEADHFMYSILQRDGLGAKDLTSTPNH

Target ORF information:

RefSeq Version	XM_015297390.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297390.1

ORF Insert Sequence:

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGAGAG GGCATGCACC AACCACTGTG GACCCCTTGC CTTCTTGTTG CAAGCAAGGA 
TCTCCTGAAG GGATGCTGGA GCAGGAGTCT GCTGTGCAGG CTGATGCTGC GACCAGCTTC 
CTGAGAGCTG CAAGATCTGG GAATCTGGAC AGAGCCTTGG ATCATCTCAG GAATGGGGTA 
GATATTAACA CCTGTAACCA GAATGGGCTG AATGCCTTGC ACCTGGCCTC CAAGGAGGGC 
CACACGAAAA TGGTGGTGGA GCTTCTGCAC AAGGAGATTG TTTTGGAGAC CACTACCAAG 
AAGGGAAACA CAGCCCTGCA CATCGCTGCC CTCGCTGGAC AGCAGGACGT GGTCCGGGAA 
CTGGTGAACT ATGGGGCCAA TGTCAATGCT CAGTCGCAGA AGGGCTTCAC ACCACTCTAC 
ATGGCAGCGC AGGAAAACCA CCTGGAAGTT GTCAAATTCC TGCTGGAAAA CGGAGCCAAC 
CAGAATGTAG CCACAGAGGA TGGCTTCACA CCACTAGCTG TGGCTCTGCA GCAGGGTCAT 
GAGAATGTGG TTGCTCACCT TATCAACTAT GGGACGAAGG GTAAGGTCCG CCTGCCTGCC 
TTGCACATTG CAGCTCGCAA CGATGACACC CGTACAGCCG CCGTGCTGCT GCAGAATGAC 
CCCAATGCTG ACGTCCTCTC CAAGACTGGA TTTACACCTT TGCACATTGC AGCCCACTAT 
GAGAATCTCA GCGTGGCCCA ATTATTGCTG AATCGTGGAG CCAGCGTCAA CTTCACACCC 
CAGAATGGGA TCACTCCTTT GCACATAGCC TCTCGTCGAG GCAACATCAT CATGGTACGG 
CTGCTGCTGG ACCGTGGGGC CCAGATAGAG ACAAGGACCA AGGATGAGTT GACGCCTCTC 
CACTGTGCAG CTCGCAATGG ACATGTGCGA ATTGCAGAGA TCCTACTGGA CCATGGTGCT 
CCCATTCAAG CCAAAACAAA GAACGGTCTG TCACCGATCC ACATGGCAGC ACAGGGTGAC 
CACCTGGACT GTGTGCGCCT ACTCCTGCAG TACAGTGCTG AGATAGACGA CATCACCCTG 
GACCACCTGA CACCACTGCA TGTAGCTGCG CACTGTGGCC ACCACCGTGT GGCCAAGCTG 
CTGGTGGAGA AGGGAGCCAA GCCCAACTCC AGAGCTCTGA ATGGCTTCAC GCCTCTCCAC 
ATTGCCTGCA AGAAGAACCA CATTCGGGTG ATGGAGTTGC TTCTGAAGAC AGGAGCCTCC 
ATTGATGCTG TCACAGAGTC TGGCCTGACC CCCTTGCATG TGGCTGCCTT CATGGGGCAC 
TTGCCCATTG TCAAAACCTT GCTGCAGCGT GGAGCATCTC CTAATGTGTC CAATGTGAAA 
GTGGAGACTC CCCTGCACAT GGCAGCCAGA GCTGGGCACA TGGATGTGGC CAAGTATCTG 
ATACAGAACA AGGCCAAAAT CAACGCCAAG GCCAAGGATG ACCAGACTCC TCTGCACTGT 
GCTGCACGCA TTGGCCACAC CAGCATGGTC CAACTCCTGC TGGAGAACAA TGCCGACCCC 
AACCTGGCCA CTACAGCAGG GCACACGCCT CTGCACATCG CTGCCAGAGA GGGGCATGTA 
GACACAGCCT TGGCCCTGCT AGAGAAGGGA GCCTCTCAGA CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TCCACGTTGC AGCCAAGTAT GGGAAGGTGG ATGTGGCAGA GCTGCTGCTG 
GTGCATGATG CTCACCCCAA TGCAGCGGGG AAGAACGGCC TGACCCCACT GCACGTAGCT 
GTGCACCACA ACAACCTGGA GATCGTCAAG CTTCTGCTTC CCAAGGGGAG CTCTCCACAC 
AGCTCAGCCT GGAACGGGTA CACCCCTCTG CACATTGCCG CCAAGCAGAA CCAGATGGAG 
GTGGCCAGCA GCTTGCTGCA GTATGGTGCT TCTGCAAATG CGGAGTCTGT TCAGGGAGTC 
ACCCCTCTGC ACCTGGCTTC TCAGGAAGGG CATACAGACA TGGTAGCACT GCTTTTCTCC 
AAGCAAGCCA ATGGAAACTT AGGCAACAAG AGTGGTCTGA CTCCTCTCCA TCTCGTGGCC 
CAAGAGGGAC ACGTACCAGT GGCTGATGTT CTGGTGAAAC ATGGAGTCAC AGTGGATGCG 
ACAACCAGGA TGGGCTACAC CTCACTGCAC ATAGCCAGCC ACTATGGGAA CATCAAGTTG 
GTGAAGTTTC TGCTGCAGCA CCAGGCTGAT GTCAATGCTA AGACTAAGCT GGGCTACACA 
CCCCTGCACC AGGCGGCACA GCAGGGCCAC ACGGATGTGG TGACCCTGCT GCTGAAGCAT 
GGTGCCTCTC CCAACGAGAT CAGCACAAAT GGCACCACTC CCCTGGCCAT TGCGAAGCGC 
CTCGGTTACA TTTCCGTCAC AGATGTGCTC AAGATAGTCA CAGAGGAGAC CACCATCCCA 
CCATTCAGTG ACAAGTACCG CATGAGCTTC CCGGAGACCG TGGATGAGAT TCTGGATGTG 
TCGGAGGATG AAGGCACTGC TCATGTCACA GTAATGGAGG AGGAGCTGGT CCCACCGAAA 
CCCAGAACAC CTGAACCCAA GGAGCAGGAG GGCAAGAGGG AGATGCTGGA GTTTGTGACC 
GTGACAACAC TGGAGCAAAT GGAGGAATCT GCAGCTGTCC CACAGGTCCC CTGCGTGCCC 
CCTGAGACTG TGGTGACCAT AGCAGAGGAG TCTGAGCAGA TGGGACCTGT GGAGACTGAG 
GCTGAGCAAG GCGGCCTGCT GCCTGCACCC TCGGTGTCCC CACAGGAGCC CTCCAAGGAG 
TTCGATGAGG ACTCCTTGAT TCCCAGCAGC CCGGCCACCG AAACCTCGGA CAACATCAGC 
CCCGTGGCCA GCCCCGTTCA CACAGGGTTC CTGGTGAGCT TCATGGTGGA TGCGCGTGGC 
GGCTCCATGC GGGGCAGCCG GCACCACGGG CTGCGCGTGG TCATCCCTCC CCGTGCCTGT 
GCTGCACCCA CCCGCATCAC TTGCCGCCTG GTGAAGCCTC AGAAGCTGCC TGCCCCCCCA 
CCGCTGGCTG AGGAGGAGGG CCTGGCAAGC CGCATCATTG CACTGGGGCC CGCTGGCGCC 
CAGTTCCTCA GCCCCGTCAT TGTGGAGATT CCTCACTTTG CCTCCTATGG ACGTGGGGAC 
CGTGAGCTGG TGGTGTTGCG CAGTGAGAAT GGCTCTGTCT GGAAGGAGCA CCGCAACCGC 
TACGAGGAGA GCTACATGGA CCAACTGCTC AGTGGCATGG ATGAGGAGCT GGAGAGCCAA 
GAGGAGCTGG AGAAGAAGAG GGTCTGCCGC ATCATCACCA CTGACTTTCC TCTCTACTTC 
GTGGTCATGT CCCGGATTTG CCAGGACTGT GACATGATTG GCCCCGAGGG AGGGTGCTTG 
AAAAGCACAC TGGTGCCCAT GGTGCAGGCC ACCTTCCCAG ACACCGCTGT CACCAAGAAA 
GTGAGGCTGG CTCTGCAGGC GCAGCCCGTG CCTGATGAGC TGGTGACCAA GCTGCTGGGG 
AACCAGGCAA CCTTCAGCCC CATCGTCACA GTGGAGCCAC GTCGGAGGAA GTTCCACCGC 
CCCATTGGCC TCCGCATCCC TCTGCCCCCA TCCTGGAAGG ACAATCCCCG GGACAGCGGC 
GAGGGTGACA CCACCAGCCT GCGCCTGCTC TGTTCTGTGA TAGGAGGGAC AGCACAAGCT 
CAGTGGGAAG ACATCACAGG CACCACAAAG CTGGTCTATG AAAAAGAGTG TGCTAACTTT 
ACCACCAATG TGTCTGCCAG GTTCTGGCTG GCTGACTGCC CGCGCACTGC TGAGGCTGTG 
CACTTTGCCA CCGTGCTGTA CAAGGAACTG ACGGCTGTGC CCTACATGGC CAAATTTGTG 
GTGTTTGCTA AGATGAACGA TGGACGGGAA GGCCGGCTGC GCTGCTACTG CATGACTGAT 
GACAAGGTGG ACAAGACTTT GGAGCATCAT GAGAACTTCA CTGAGGTGGC CCGCAGCAGG 
GACATTGAGG TGGTGGAAGG AATGCCTCTG CATGTCGAGC TCTCAGGGAA CCTGGTGCCC 
GTCAAGAAGG CCACGCAGTC ACGCACCTTC TTCTTCCAGT CCTTCCGGGA AAGCCGCCTC 
GCCATCCCCA TCAAGGTCCG GGACAGCAGC CGGGAAGCCA GTGGCTCCCT GTCCTTCCTG 
CGCAAGGCCA TGAAGTATGA GGACTTGCAG CATGTGCTCT GCCACCTCAA CATCAGCATT 
CCACCCTGCA CCAAGGGAAG CAGCACTGAG GAGCGAAGGA GGACACTGAC ACCTTTGTCT 
CTGCGCTCTC TGAGCAGCAC CGACAGGGCA GACCAGAAGA TGATCGACAT TGCAGAGCAG 
CTTGGCCTCA GCTGGGCAGA GCTGGCACGT GAGCTGCAGT TTGGGGTGGA TGACATCAAC 
AGGATTCGTG TGGAGAACCC TAACTCCTTG CTGGAGCAGA GCATAGCCTT GCTTAACCTC 
TGGGTCAGCC GCGAGGGCAA GGGTGTCAAA ATTGAGAACC TGTACACAGC GCTGAGGAAC 
ATTGACCGCA GCGAGATCAT CAGCACGTTG GAGGGCTCCG GCCGGCAGAG CCGCAGCATG 
AAGGGCAGCT GGCGTTACAT GGACAGGGAC TATTCACTCT CGCCCTCCCA GATGAATGGT 
TACGCTTCGC TGCAGGACGA GCTGCTCTCC CCTGCCTCCC TGCATTATAT GCTGCCATCC 
CCTCTGCGTG CTGACCAGTA CTGGAACGAG GTGGCCATCA TGGACGCCAT CCCCATGGCC 
GCCACTGAGC AGGACGCCCT GATGGAGATG TCTGACATGC AGGTGTGGTC CTCAGGGCTC 
ACCCCTTCAC TGGTGACGGC TGAGGACTCC TCTCTGGAGT GCAGCAAGGC TGAGGACTCG 
GATGCTACAA GCGAAGGTCG TTTCCCAGGG CACCTTCTGA CAGATGCACA TGTCCCAGAC 
CACATGGGCT CTATGGACTT AGTTGAGGAT GACACAGTGG ACTCAGACGC CATGAACGGG 
CTGATTGAAC TGCTAGAGCA GGAGGAGGGG CAGAGGCCAG AGGGGAAGAT GCCAGCCAGC 
GACCACCAGC TAGGGACTGG AGAGCAGAAC CCAGAGAGTG AAGTCTCTTT TGTTTCAGTT 
CAACAGAAGG TACAAGCCAC GATCGTGCCA TCGCCAACCG TGAGCCACAC AGTGGAGAGG 
AGTGCAGACA GGCTGAGGGA CTGGAATGCA GAAGGCTCCT TTATCTCCTG CCTACAGGAC 
CTGACAGAGG GCTCCTGGCA GGAGGGGGTC ACCCGACGGC TGGGTCCAGC ACACACCGTG 
GCTGCTGGGG CGCAGGGCCA GGAGCAAGAG CAGGTCCTGG CGCCGGACGT GGAGCTGATG 
CGGGTCAGCT CCACAGAGGA CAGCGACTGG CAGCCCCAGC ACCCCGCGGG CGGCTGGCCA 
GAGGAGCCTG ACAGCCGCTG CTTTGGGCAG GGCAACGAAG TCCTCCATCT CCCTGGGGAG 
CATGTGACTG AGGAGCAGTT CACAGATGAA CAAGGCAATA TAATCACCAA GAAGGTCATC 
CGGAAAGTGG TGCATCAGTT GGACCTCGAC ACGGATGACA GGAAGGAGCA TGAGGACTTG 
ATTCTGGAGG GCTCCCTGCA GGAGCCCCAA GACCTGGAGG CAGAGGCTGA TCACTTCATG 
TACTCCATCC TACAGCGGGA CGGTCTGGGG GCCAAGGATC TCACCTCAAC ACCAAACCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK1 ( XM_015297391.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152877.1 Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X3, mRNA.

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CloneID	OGa45387
Clone ID Related Accession (Same CDS sequence)	XM_015297391.1
Accession Version	XM_015297391.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ankyrin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455999.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGGTCA GTGATGCTGG GGCTGATGCT GCGACCAGCT TCCTGAGAGC TGCAAGATCT 
GGGAATCTGG ACAGAGCCTT GGATCATCTC AGGAATGGGG TAGATATTAA CACCTGTAAC 
CAGAATGGGC TGAATGCCTT GCACCTGGCC TCCAAGGAGG GCCACACGAA AATGGTGGTG 
GAGCTTCTGC ACAAGGAGAT TGTTTTGGAG ACCACTACCA AGAAGGGAAA CACAGCCCTG 
CACATCGCTG CCCTCGCTGG ACAGCAGGAC GTGGTCCGGG AACTGGTGAA CTATGGGGCC 
AATGTCAATG CTCAGTCGCA GAAGGGCTTC ACACCACTCT ACATGGCAGC GCAGGAAAAC 
CACCTGGAAG TTGTCAAATT CCTGCTGGAA AACGGAGCCA ACCAGAATGT AGCCACAGAG 
GATGGCTTCA CACCACTAGC TGTGGCTCTG CAGCAGGGTC ATGAGAATGT GGTTGCTCAC 
CTTATCAACT ATGGGACGAA GGGTAAGGTC CGCCTGCCTG CCTTGCACAT TGCAGCTCGC 
AACGATGACA CCCGTACAGC CGCCGTGCTG CTGCAGAATG ACCCCAATGC TGACGTCCTC 
TCCAAGACTG GATTTACACC TTTGCACATT GCAGCCCACT ATGAGAATCT CAGCGTGGCC 
CAATTATTGC TGAATCGTGG AGCCAGCGTC AACTTCACAC CCCAGAATGG GATCACTCCT 
TTGCACATAG CCTCTCGTCG AGGCAACATC ATCATGGTAC GGCTGCTGCT GGACCGTGGG 
GCCCAGATAG AGACAAGGAC CAAGGATGAG TTGACGCCTC TCCACTGTGC AGCTCGCAAT 
GGACATGTGC GAATTGCAGA GATCCTACTG GACCATGGTG CTCCCATTCA AGCCAAAACA 
AAGAACGGTC TGTCACCGAT CCACATGGCA GCACAGGGTG ACCACCTGGA CTGTGTGCGC 
CTACTCCTGC AGTACAGTGC TGAGATAGAC GACATCACCC TGGACCACCT GACACCACTG 
CATGTAGCTG CGCACTGTGG CCACCACCGT GTGGCCAAGC TGCTGGTGGA GAAGGGAGCC 
AAGCCCAACT CCAGAGCTCT GAATGGCTTC ACGCCTCTCC ACATTGCCTG CAAGAAGAAC 
CACATTCGGG TGATGGAGTT GCTTCTGAAG ACAGGAGCCT CCATTGATGC TGTCACAGAG 
TCTGGCCTGA CCCCCTTGCA TGTGGCTGCC TTCATGGGGC ACTTGCCCAT TGTCAAAACC 
TTGCTGCAGC GTGGAGCATC TCCTAATGTG TCCAATGTGA AAGTGGAGAC TCCCCTGCAC 
ATGGCAGCCA GAGCTGGGCA CATGGATGTG GCCAAGTATC TGATACAGAA CAAGGCCAAA 
ATCAACGCCA AGGCCAAGGA TGACCAGACT CCTCTGCACT GTGCTGCACG CATTGGCCAC 
ACCAGCATGG TCCAACTCCT GCTGGAGAAC AATGCCGACC CCAACCTGGC CACTACAGCA 
GGGCACACGC CTCTGCACAT CGCTGCCAGA GAGGGGCATG TAGACACAGC CTTGGCCCTG 
CTAGAGAAGG GAGCCTCTCA GACCTGCATG ACCAAGAAAG GATTTACCCC TCTCCACGTT 
GCAGCCAAGT ATGGGAAGGT GGATGTGGCA GAGCTGCTGC TGGTGCATGA TGCTCACCCC 
AATGCAGCGG GGAAGAACGG CCTGACCCCA CTGCACGTAG CTGTGCACCA CAACAACCTG 
GAGATCGTCA AGCTTCTGCT TCCCAAGGGG AGCTCTCCAC ACAGCTCAGC CTGGAACGGG 
TACACCCCTC TGCACATTGC CGCCAAGCAG AACCAGATGG AGGTGGCCAG CAGCTTGCTG 
CAGTATGGTG CTTCTGCAAA TGCGGAGTCT GTTCAGGGAG TCACCCCTCT GCACCTGGCT 
TCTCAGGAAG GGCATACAGA CATGGTAGCA CTGCTTTTCT CCAAGCAAGC CAATGGAAAC 
TTAGGCAACA AGAGTGGTCT GACTCCTCTC CATCTCGTGG CCCAAGAGGG ACACGTACCA 
GTGGCTGATG TTCTGGTGAA ACATGGAGTC ACAGTGGATG CGACAACCAG GATGGGCTAC 
ACCTCACTGC ACATAGCCAG CCACTATGGG AACATCAAGT TGGTGAAGTT TCTGCTGCAG 
CACCAGGCTG ATGTCAATGC TAAGACTAAG CTGGGCTACA CACCCCTGCA CCAGGCGGCA 
CAGCAGGGCC ACACGGATGT GGTGACCCTG CTGCTGAAGC ATGGTGCCTC TCCCAACGAG 
ATCAGCACAA ATGGCACCAC TCCCCTGGCC ATTGCGAAGC GCCTCGGTTA CATTTCCGTC 
ACAGATGTGC TCAAGATAGT CACAGAGGAG ACCACCATCC CACCATTCAG TGACAAGTAC 
CGCATGAGCT TCCCGGAGAC CGTGGATGAG ATTCTGGATG TGTCGGAGGA TGAAGGCACT 
GCTCATGTCA CAGTAATGGA GGAGGAGCTG GTCCCACCGA AACCCAGAAC ACCTGAACCC 
AAGGAGCAGG AGGGCAAGAG GGAGATGCTG GAGTTTGTGA CCGTGACAAC ACTGGAGCAA 
ATGGAGGAAT CTGCAGCTGT CCCACAGGTC CCCTGCGTGC CCCCTGAGAC TGTGGTGACC 
ATAGCAGAGG AGTCTGAGCA GATGGGACCT GTGGAGACTG AGGCTGAGCA AGGCGGCCTG 
CTGCCTGCAC CCTCGGTGTC CCCACAGGAG CCCTCCAAGG AGTTCGATGA GGACTCCTTG 
ATTCCCAGCA GCCCGGCCAC CGAAACCTCG GACAACATCA GCCCCGTGGC CAGCCCCGTT 
CACACAGGGT TCCTGGTGAG CTTCATGGTG GATGCGCGTG GCGGCTCCAT GCGGGGCAGC 
CGGCACCACG GGCTGCGCGT GGTCATCCCT CCCCGTGCCT GTGCTGCACC CACCCGCATC 
ACTTGCCGCC TGGTGAAGCC TCAGAAGCTG CCTGCCCCCC CACCGCTGGC TGAGGAGGAG 
GGCCTGGCAA GCCGCATCAT TGCACTGGGG CCCGCTGGCG CCCAGTTCCT CAGCCCCGTC 
ATTGTGGAGA TTCCTCACTT TGCCTCCTAT GGACGTGGGG ACCGTGAGCT GGTGGTGTTG 
CGCAGTGAGA ATGGCTCTGT CTGGAAGGAG CACCGCAACC GCTACGAGGA GAGCTACATG 
GACCAACTGC TCAGTGGCAT GGATGAGGAG CTGGAGAGCC AAGAGGAGCT GGAGAAGAAG 
AGGGTCTGCC GCATCATCAC CACTGACTTT CCTCTCTACT TCGTGGTCAT GTCCCGGATT 
TGCCAGGACT GTGACATGAT TGGCCCCGAG GGAGGGTGCT TGAAAAGCAC ACTGGTGCCC 
ATGGTGCAGG CCACCTTCCC AGACACCGCT GTCACCAAGA AAGTGAGGCT GGCTCTGCAG 
GCGCAGCCCG TGCCTGATGA GCTGGTGACC AAGCTGCTGG GGAACCAGGC AACCTTCAGC 
CCCATCGTCA CAGTGGAGCC ACGTCGGAGG AAGTTCCACC GCCCCATTGG CCTCCGCATC 
CCTCTGCCCC CATCCTGGAA GGACAATCCC CGGGACAGCG GCGAGGGTGA CACCACCAGC 
CTGCGCCTGC TCTGTTCTGT GATAGGAGGG ACAGCACAAG CTCAGTGGGA AGACATCACA 
GGCACCACAA AGCTGGTCTA TGAAAAAGAG TGTGCTAACT TTACCACCAA TGTGTCTGCC 
AGGTTCTGGC TGGCTGACTG CCCGCGCACT GCTGAGGCTG TGCACTTTGC CACCGTGCTG 
TACAAGGAAC TGACGGCTGT GCCCTACATG GCCAAATTTG TGGTGTTTGC TAAGATGAAC 
GATGGACGGG AAGGCCGGCT GCGCTGCTAC TGCATGACTG ATGACAAGGT GGACAAGACT 
TTGGAGCATC ATGAGAACTT CACTGAGGTG GCCCGCAGCA GGGACATTGA GGTGGTGGAA 
GGAATGCCTC TGCATGTCGA GCTCTCAGGG AACCTGGTGC CCGTCAAGAA GGCCACGCAG 
TCACGCACCT TCTTCTTCCA GTCCTTCCGG GAAAGCCGCC TCGCCATCCC CATCAAGGTC 
CGGGACAGCA GCCGGGAAGC CAGTGGCTCC CTGTCCTTCC TGCGCAAGGC CATGAAGTAT 
GAGGACTTGC AGCATGTGCT CTGCCACCTC AACATCAGCA TTCCACCCTG CACCAAGGGA 
AGCAGCACTG AGGAGCGAAG GAGGACACTG ACACCTTTGT CTCTGCGCTC TCTGAGCAGC 
ACCGACAGGG CAGACCAGAA GATGATCGAC ATTGCAGAGC AGCTTGGCCT CAGCTGGGCA 
GAGCTGGCAC GTGAGCTGCA GTTTGGGGTG GATGACATCA ACAGGATTCG TGTGGAGAAC 
CCTAACTCCT TGCTGGAGCA GAGCATAGCC TTGCTTAACC TCTGGGTCAG CCGCGAGGGC 
AAGGGTGTCA AAATTGAGAA CCTGTACACA GCGCTGAGGA ACATTGACCG CAGCGAGATC 
ATCAGCACGT TGGAGGGCTC CGGCCGGCAG AGCCGCAGCA TGAAGGGCAG CTGGCGTTAC 
ATGGACAGGG ACTATTCACT CTCGCCCTCC CAGATGAATG GTTACGCTTC GCTGCAGGAC 
GAGCTGCTCT CCCCTGCCTC CCTGCATTAT ATGCTGCCAT CCCCTCTGCG TGCTGACCAG 
TACTGGAACG AGGTGGCCAT CATGGACGCC ATCCCCATGG CCGCCACTGA GCAGGACGCC 
CTGATGGAGA TGTCTGACAT GCAGGTGTGG TCCTCAGGGC TCACCCCTTC ACTGGTGACG 
GCTGAGGACT CCTCTCTGGA GTGCAGCAAG GCTGAGGACT CGGATGCTAC AAGCGAAGGT 
CGTTTCCCAG GGCACCTTCT GACAGATGCA CATGTCCCAG ACCACATGGG CTCTATGGAC 
TTAGTTGAGG ATGACACAGT GGACTCAGAC GCCATGAACG GGCTGATTGA ACTGCTAGAG 
CAGGAGGAGG GGCAGAGGCC AGAGGGGAAG ATGCCAGCCA GCGACCACCA GCTAGGGACT 
GGAGAGCAGA ACCCAGAGAG TGAAGTCTCT TTTGTTTCAG TTCAACAGAA GGTACAAGCC 
ACGATCGTGC CATCGCCAAC CGTGAGCCAC ACAGTGGAGA GGAGTGCAGA CAGGCTGAGG 
GACTGGAATG CAGAAGGCTC CTTTATCTCC TGCCTACAGG ACCTGACAGA GGGCTCCTGG 
CAGGAGGGGG TCACCCGACG GCTGGGTCCA GCACACACCG TGGCTGCTGG GGCGCAGGGC 
CAGGAGCAAG AGCAGGTCCT GGCGCCGGAC GTGGAGCTGA TGCGGGTCAG CTCCACAGAG 
GACAGCGACT GGCAGCCCCA GCACCCCGCG GGCGGCTGGC CAGAGGAGCC TGACAGCCGC 
TGCTTTGGGC AGGGCAACGA AGTCCTCCAT CTCCCTGGGG AGCATGTGAC TGAGGAGCAG 
TTCACAGATG AACAAGGCAA TATAATCACC AAGAAGGTCA TCCGGAAAGT GGTGCATCAG 
TTGGACCTCG ACACGGATGA CAGGAAGGAG CATGAGGACT TGATTCTGGA GGGCTCCCTG 
CAGGAGCCCC AAGACCTGGA GGCAGAGGCT GATCACTTCA TGTACTCCAT CCTACAGCGG 
GACGGTCTGG GGGCCAAGGA GGAGGTGCAA GTGCGTGTCC CAAAACTGGA AGTCTCCGGG 
AGCAGGATGG GGGCTCAGAT AGTGAAACGA GCCAGTCTGA AAAGGGGGAA GCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152877.1
CDS	871..6747
Translation	MAQAAKQLKKIKDIEAQALQEQKEKEESNRKRRNRSRDRKKKVSDAGADAATSFLRAARSGNLDRALDHLRNGVDINTCNQNGLNALHLASKEGHTKMVVELLHKEIVLETTTKKGNTALHIAALAGQQDVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLSVAQLLLNRGASVNFTPQNGITPLHIASRRGNIIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRIAEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYSAEIDDITLDHLTPLHVAAHCGHHRVAKLLVEKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVAAFMGHLPIVKTLLQRGASPNVSNVKVETPLHMAARAGHMDVAKYLIQNKAKINAKAKDDQTPLHCAARIGHTSMVQLLLENNADPNLATTAGHTPLHIAAREGHVDTALALLEKGASQTCMTKKGFTPLHVAAKYGKVDVAELLLVHDAHPNAAGKNGLTPLHVAVHHNNLEIVKLLLPKGSSPHSSAWNGYTPLHIAAKQNQMEVASSLLQYGASANAESVQGVTPLHLASQEGHTDMVALLFSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLVKHGVTVDATTRMGYTSLHIASHYGNIKLVKFLLQHQADVNAKTKLGYTPLHQAAQQGHTDVVTLLLKHGASPNEISTNGTTPLAIAKRLGYISVTDVLKIVTEETTIPPFSDKYRMSFPETVDEILDVSEDEGTAHVTVMEEELVPPKPRTPEPKEQEGKREMLEFVTVTTLEQMEESAAVPQVPCVPPETVVTIAEESEQMGPVETEAEQGGLLPAPSVSPQEPSKEFDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHHGLRVVIPPRACAAPTRITCRLVKPQKLPAPPPLAEEEGLASRIIALGPAGAQFLSPVIVEIPHFASYGRGDRELVVLRSENGSVWKEHRNRYEESYMDQLLSGMDEELESQEELEKKRVCRIITTDFPLYFVVMSRICQDCDMIGPEGGCLKSTLVPMVQATFPDTAVTKKVRLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWKDNPRDSGEGDTTSLRLLCSVIGGTAQAQWEDITGTTKLVYEKECANFTTNVSARFWLADCPRTAEAVHFATVLYKELTAVPYMAKFVVFAKMNDGREGRLRCYCMTDDKVDKTLEHHENFTEVARSRDIEVVEGMPLHVELSGNLVPVKKATQSRTFFFQSFRESRLAIPIKVRDSSREASGSLSFLRKAMKYEDLQHVLCHLNISIPPCTKGSSTEERRRTLTPLSLRSLSSTDRADQKMIDIAEQLGLSWAELARELQFGVDDINRIRVENPNSLLEQSIALLNLWVSREGKGVKIENLYTALRNIDRSEIISTLEGSGRQSRSMKGSWRYMDRDYSLSPSQMNGYASLQDELLSPASLHYMLPSPLRADQYWNEVAIMDAIPMAATEQDALMEMSDMQVWSSGLTPSLVTAEDSSLECSKAEDSDATSEGRFPGHLLTDAHVPDHMGSMDLVEDDTVDSDAMNGLIELLEQEEGQRPEGKMPASDHQLGTGEQNPESEVSFVSVQQKVQATIVPSPTVSHTVERSADRLRDWNAEGSFISCLQDLTEGSWQEGVTRRLGPAHTVAAGAQGQEQEQVLAPDVELMRVSSTEDSDWQPQHPAGGWPEEPDSRCFGQGNEVLHLPGEHVTEEQFTDEQGNIITKKVIRKVVHQLDLDTDDRKEHEDLILEGSLQEPQDLEAEADHFMYSILQRDGLGAKEEVQVRVPKLEVSGSRMGAQIVKRASLKRGKH

Target ORF information:

RefSeq Version	XM_015297391.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297391.1

ORF Insert Sequence:

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGGTCA GTGATGCTGG GGCTGATGCT GCGACCAGCT TCCTGAGAGC TGCAAGATCT 
GGGAATCTGG ACAGAGCCTT GGATCATCTC AGGAATGGGG TAGATATTAA CACCTGTAAC 
CAGAATGGGC TGAATGCCTT GCACCTGGCC TCCAAGGAGG GCCACACGAA AATGGTGGTG 
GAGCTTCTGC ACAAGGAGAT TGTTTTGGAG ACCACTACCA AGAAGGGAAA CACAGCCCTG 
CACATCGCTG CCCTCGCTGG ACAGCAGGAC GTGGTCCGGG AACTGGTGAA CTATGGGGCC 
AATGTCAATG CTCAGTCGCA GAAGGGCTTC ACACCACTCT ACATGGCAGC GCAGGAAAAC 
CACCTGGAAG TTGTCAAATT CCTGCTGGAA AACGGAGCCA ACCAGAATGT AGCCACAGAG 
GATGGCTTCA CACCACTAGC TGTGGCTCTG CAGCAGGGTC ATGAGAATGT GGTTGCTCAC 
CTTATCAACT ATGGGACGAA GGGTAAGGTC CGCCTGCCTG CCTTGCACAT TGCAGCTCGC 
AACGATGACA CCCGTACAGC CGCCGTGCTG CTGCAGAATG ACCCCAATGC TGACGTCCTC 
TCCAAGACTG GATTTACACC TTTGCACATT GCAGCCCACT ATGAGAATCT CAGCGTGGCC 
CAATTATTGC TGAATCGTGG AGCCAGCGTC AACTTCACAC CCCAGAATGG GATCACTCCT 
TTGCACATAG CCTCTCGTCG AGGCAACATC ATCATGGTAC GGCTGCTGCT GGACCGTGGG 
GCCCAGATAG AGACAAGGAC CAAGGATGAG TTGACGCCTC TCCACTGTGC AGCTCGCAAT 
GGACATGTGC GAATTGCAGA GATCCTACTG GACCATGGTG CTCCCATTCA AGCCAAAACA 
AAGAACGGTC TGTCACCGAT CCACATGGCA GCACAGGGTG ACCACCTGGA CTGTGTGCGC 
CTACTCCTGC AGTACAGTGC TGAGATAGAC GACATCACCC TGGACCACCT GACACCACTG 
CATGTAGCTG CGCACTGTGG CCACCACCGT GTGGCCAAGC TGCTGGTGGA GAAGGGAGCC 
AAGCCCAACT CCAGAGCTCT GAATGGCTTC ACGCCTCTCC ACATTGCCTG CAAGAAGAAC 
CACATTCGGG TGATGGAGTT GCTTCTGAAG ACAGGAGCCT CCATTGATGC TGTCACAGAG 
TCTGGCCTGA CCCCCTTGCA TGTGGCTGCC TTCATGGGGC ACTTGCCCAT TGTCAAAACC 
TTGCTGCAGC GTGGAGCATC TCCTAATGTG TCCAATGTGA AAGTGGAGAC TCCCCTGCAC 
ATGGCAGCCA GAGCTGGGCA CATGGATGTG GCCAAGTATC TGATACAGAA CAAGGCCAAA 
ATCAACGCCA AGGCCAAGGA TGACCAGACT CCTCTGCACT GTGCTGCACG CATTGGCCAC 
ACCAGCATGG TCCAACTCCT GCTGGAGAAC AATGCCGACC CCAACCTGGC CACTACAGCA 
GGGCACACGC CTCTGCACAT CGCTGCCAGA GAGGGGCATG TAGACACAGC CTTGGCCCTG 
CTAGAGAAGG GAGCCTCTCA GACCTGCATG ACCAAGAAAG GATTTACCCC TCTCCACGTT 
GCAGCCAAGT ATGGGAAGGT GGATGTGGCA GAGCTGCTGC TGGTGCATGA TGCTCACCCC 
AATGCAGCGG GGAAGAACGG CCTGACCCCA CTGCACGTAG CTGTGCACCA CAACAACCTG 
GAGATCGTCA AGCTTCTGCT TCCCAAGGGG AGCTCTCCAC ACAGCTCAGC CTGGAACGGG 
TACACCCCTC TGCACATTGC CGCCAAGCAG AACCAGATGG AGGTGGCCAG CAGCTTGCTG 
CAGTATGGTG CTTCTGCAAA TGCGGAGTCT GTTCAGGGAG TCACCCCTCT GCACCTGGCT 
TCTCAGGAAG GGCATACAGA CATGGTAGCA CTGCTTTTCT CCAAGCAAGC CAATGGAAAC 
TTAGGCAACA AGAGTGGTCT GACTCCTCTC CATCTCGTGG CCCAAGAGGG ACACGTACCA 
GTGGCTGATG TTCTGGTGAA ACATGGAGTC ACAGTGGATG CGACAACCAG GATGGGCTAC 
ACCTCACTGC ACATAGCCAG CCACTATGGG AACATCAAGT TGGTGAAGTT TCTGCTGCAG 
CACCAGGCTG ATGTCAATGC TAAGACTAAG CTGGGCTACA CACCCCTGCA CCAGGCGGCA 
CAGCAGGGCC ACACGGATGT GGTGACCCTG CTGCTGAAGC ATGGTGCCTC TCCCAACGAG 
ATCAGCACAA ATGGCACCAC TCCCCTGGCC ATTGCGAAGC GCCTCGGTTA CATTTCCGTC 
ACAGATGTGC TCAAGATAGT CACAGAGGAG ACCACCATCC CACCATTCAG TGACAAGTAC 
CGCATGAGCT TCCCGGAGAC CGTGGATGAG ATTCTGGATG TGTCGGAGGA TGAAGGCACT 
GCTCATGTCA CAGTAATGGA GGAGGAGCTG GTCCCACCGA AACCCAGAAC ACCTGAACCC 
AAGGAGCAGG AGGGCAAGAG GGAGATGCTG GAGTTTGTGA CCGTGACAAC ACTGGAGCAA 
ATGGAGGAAT CTGCAGCTGT CCCACAGGTC CCCTGCGTGC CCCCTGAGAC TGTGGTGACC 
ATAGCAGAGG AGTCTGAGCA GATGGGACCT GTGGAGACTG AGGCTGAGCA AGGCGGCCTG 
CTGCCTGCAC CCTCGGTGTC CCCACAGGAG CCCTCCAAGG AGTTCGATGA GGACTCCTTG 
ATTCCCAGCA GCCCGGCCAC CGAAACCTCG GACAACATCA GCCCCGTGGC CAGCCCCGTT 
CACACAGGGT TCCTGGTGAG CTTCATGGTG GATGCGCGTG GCGGCTCCAT GCGGGGCAGC 
CGGCACCACG GGCTGCGCGT GGTCATCCCT CCCCGTGCCT GTGCTGCACC CACCCGCATC 
ACTTGCCGCC TGGTGAAGCC TCAGAAGCTG CCTGCCCCCC CACCGCTGGC TGAGGAGGAG 
GGCCTGGCAA GCCGCATCAT TGCACTGGGG CCCGCTGGCG CCCAGTTCCT CAGCCCCGTC 
ATTGTGGAGA TTCCTCACTT TGCCTCCTAT GGACGTGGGG ACCGTGAGCT GGTGGTGTTG 
CGCAGTGAGA ATGGCTCTGT CTGGAAGGAG CACCGCAACC GCTACGAGGA GAGCTACATG 
GACCAACTGC TCAGTGGCAT GGATGAGGAG CTGGAGAGCC AAGAGGAGCT GGAGAAGAAG 
AGGGTCTGCC GCATCATCAC CACTGACTTT CCTCTCTACT TCGTGGTCAT GTCCCGGATT 
TGCCAGGACT GTGACATGAT TGGCCCCGAG GGAGGGTGCT TGAAAAGCAC ACTGGTGCCC 
ATGGTGCAGG CCACCTTCCC AGACACCGCT GTCACCAAGA AAGTGAGGCT GGCTCTGCAG 
GCGCAGCCCG TGCCTGATGA GCTGGTGACC AAGCTGCTGG GGAACCAGGC AACCTTCAGC 
CCCATCGTCA CAGTGGAGCC ACGTCGGAGG AAGTTCCACC GCCCCATTGG CCTCCGCATC 
CCTCTGCCCC CATCCTGGAA GGACAATCCC CGGGACAGCG GCGAGGGTGA CACCACCAGC 
CTGCGCCTGC TCTGTTCTGT GATAGGAGGG ACAGCACAAG CTCAGTGGGA AGACATCACA 
GGCACCACAA AGCTGGTCTA TGAAAAAGAG TGTGCTAACT TTACCACCAA TGTGTCTGCC 
AGGTTCTGGC TGGCTGACTG CCCGCGCACT GCTGAGGCTG TGCACTTTGC CACCGTGCTG 
TACAAGGAAC TGACGGCTGT GCCCTACATG GCCAAATTTG TGGTGTTTGC TAAGATGAAC 
GATGGACGGG AAGGCCGGCT GCGCTGCTAC TGCATGACTG ATGACAAGGT GGACAAGACT 
TTGGAGCATC ATGAGAACTT CACTGAGGTG GCCCGCAGCA GGGACATTGA GGTGGTGGAA 
GGAATGCCTC TGCATGTCGA GCTCTCAGGG AACCTGGTGC CCGTCAAGAA GGCCACGCAG 
TCACGCACCT TCTTCTTCCA GTCCTTCCGG GAAAGCCGCC TCGCCATCCC CATCAAGGTC 
CGGGACAGCA GCCGGGAAGC CAGTGGCTCC CTGTCCTTCC TGCGCAAGGC CATGAAGTAT 
GAGGACTTGC AGCATGTGCT CTGCCACCTC AACATCAGCA TTCCACCCTG CACCAAGGGA 
AGCAGCACTG AGGAGCGAAG GAGGACACTG ACACCTTTGT CTCTGCGCTC TCTGAGCAGC 
ACCGACAGGG CAGACCAGAA GATGATCGAC ATTGCAGAGC AGCTTGGCCT CAGCTGGGCA 
GAGCTGGCAC GTGAGCTGCA GTTTGGGGTG GATGACATCA ACAGGATTCG TGTGGAGAAC 
CCTAACTCCT TGCTGGAGCA GAGCATAGCC TTGCTTAACC TCTGGGTCAG CCGCGAGGGC 
AAGGGTGTCA AAATTGAGAA CCTGTACACA GCGCTGAGGA ACATTGACCG CAGCGAGATC 
ATCAGCACGT TGGAGGGCTC CGGCCGGCAG AGCCGCAGCA TGAAGGGCAG CTGGCGTTAC 
ATGGACAGGG ACTATTCACT CTCGCCCTCC CAGATGAATG GTTACGCTTC GCTGCAGGAC 
GAGCTGCTCT CCCCTGCCTC CCTGCATTAT ATGCTGCCAT CCCCTCTGCG TGCTGACCAG 
TACTGGAACG AGGTGGCCAT CATGGACGCC ATCCCCATGG CCGCCACTGA GCAGGACGCC 
CTGATGGAGA TGTCTGACAT GCAGGTGTGG TCCTCAGGGC TCACCCCTTC ACTGGTGACG 
GCTGAGGACT CCTCTCTGGA GTGCAGCAAG GCTGAGGACT CGGATGCTAC AAGCGAAGGT 
CGTTTCCCAG GGCACCTTCT GACAGATGCA CATGTCCCAG ACCACATGGG CTCTATGGAC 
TTAGTTGAGG ATGACACAGT GGACTCAGAC GCCATGAACG GGCTGATTGA ACTGCTAGAG 
CAGGAGGAGG GGCAGAGGCC AGAGGGGAAG ATGCCAGCCA GCGACCACCA GCTAGGGACT 
GGAGAGCAGA ACCCAGAGAG TGAAGTCTCT TTTGTTTCAG TTCAACAGAA GGTACAAGCC 
ACGATCGTGC CATCGCCAAC CGTGAGCCAC ACAGTGGAGA GGAGTGCAGA CAGGCTGAGG 
GACTGGAATG CAGAAGGCTC CTTTATCTCC TGCCTACAGG ACCTGACAGA GGGCTCCTGG 
CAGGAGGGGG TCACCCGACG GCTGGGTCCA GCACACACCG TGGCTGCTGG GGCGCAGGGC 
CAGGAGCAAG AGCAGGTCCT GGCGCCGGAC GTGGAGCTGA TGCGGGTCAG CTCCACAGAG 
GACAGCGACT GGCAGCCCCA GCACCCCGCG GGCGGCTGGC CAGAGGAGCC TGACAGCCGC 
TGCTTTGGGC AGGGCAACGA AGTCCTCCAT CTCCCTGGGG AGCATGTGAC TGAGGAGCAG 
TTCACAGATG AACAAGGCAA TATAATCACC AAGAAGGTCA TCCGGAAAGT GGTGCATCAG 
TTGGACCTCG ACACGGATGA CAGGAAGGAG CATGAGGACT TGATTCTGGA GGGCTCCCTG 
CAGGAGCCCC AAGACCTGGA GGCAGAGGCT GATCACTTCA TGTACTCCAT CCTACAGCGG 
GACGGTCTGG GGGCCAAGGA GGAGGTGCAA GTGCGTGTCC CAAAACTGGA AGTCTCCGGG 
AGCAGGATGG GGGCTCAGAT AGTGAAACGA GCCAGTCTGA AAAGGGGGAA GCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK1 ( XM_015297393.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152879.1 Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X4, mRNA.

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CloneID	OGa45388
Clone ID Related Accession (Same CDS sequence)	XM_015297393.1
Accession Version	XM_015297393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ankyrin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455999.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGGCTG ATGCTGCGAC CAGCTTCCTG AGAGCTGCAA GATCTGGGAA TCTGGACAGA 
GCCTTGGATC ATCTCAGGAA TGGGGTAGAT ATTAACACCT GTAACCAGAA TGGGCTGAAT 
GCCTTGCACC TGGCCTCCAA GGAGGGCCAC ACGAAAATGG TGGTGGAGCT TCTGCACAAG 
GAGATTGTTT TGGAGACCAC TACCAAGAAG GGAAACACAG CCCTGCACAT CGCTGCCCTC 
GCTGGACAGC AGGACGTGGT CCGGGAACTG GTGAACTATG GGGCCAATGT CAATGCTCAG 
TCGCAGAAGG GCTTCACACC ACTCTACATG GCAGCGCAGG AAAACCACCT GGAAGTTGTC 
AAATTCCTGC TGGAAAACGG AGCCAACCAG AATGTAGCCA CAGAGGATGG CTTCACACCA 
CTAGCTGTGG CTCTGCAGCA GGGTCATGAG AATGTGGTTG CTCACCTTAT CAACTATGGG 
ACGAAGGGTA AGGTCCGCCT GCCTGCCTTG CACATTGCAG CTCGCAACGA TGACACCCGT 
ACAGCCGCCG TGCTGCTGCA GAATGACCCC AATGCTGACG TCCTCTCCAA GACTGGATTT 
ACACCTTTGC ACATTGCAGC CCACTATGAG AATCTCAGCG TGGCCCAATT ATTGCTGAAT 
CGTGGAGCCA GCGTCAACTT CACACCCCAG AATGGGATCA CTCCTTTGCA CATAGCCTCT 
CGTCGAGGCA ACATCATCAT GGTACGGCTG CTGCTGGACC GTGGGGCCCA GATAGAGACA 
AGGACCAAGG ATGAGTTGAC GCCTCTCCAC TGTGCAGCTC GCAATGGACA TGTGCGAATT 
GCAGAGATCC TACTGGACCA TGGTGCTCCC ATTCAAGCCA AAACAAAGAA CGGTCTGTCA 
CCGATCCACA TGGCAGCACA GGGTGACCAC CTGGACTGTG TGCGCCTACT CCTGCAGTAC 
AGTGCTGAGA TAGACGACAT CACCCTGGAC CACCTGACAC CACTGCATGT AGCTGCGCAC 
TGTGGCCACC ACCGTGTGGC CAAGCTGCTG GTGGAGAAGG GAGCCAAGCC CAACTCCAGA 
GCTCTGAATG GCTTCACGCC TCTCCACATT GCCTGCAAGA AGAACCACAT TCGGGTGATG 
GAGTTGCTTC TGAAGACAGG AGCCTCCATT GATGCTGTCA CAGAGTCTGG CCTGACCCCC 
TTGCATGTGG CTGCCTTCAT GGGGCACTTG CCCATTGTCA AAACCTTGCT GCAGCGTGGA 
GCATCTCCTA ATGTGTCCAA TGTGAAAGTG GAGACTCCCC TGCACATGGC AGCCAGAGCT 
GGGCACATGG ATGTGGCCAA GTATCTGATA CAGAACAAGG CCAAAATCAA CGCCAAGGCC 
AAGGATGACC AGACTCCTCT GCACTGTGCT GCACGCATTG GCCACACCAG CATGGTCCAA 
CTCCTGCTGG AGAACAATGC CGACCCCAAC CTGGCCACTA CAGCAGGGCA CACGCCTCTG 
CACATCGCTG CCAGAGAGGG GCATGTAGAC ACAGCCTTGG CCCTGCTAGA GAAGGGAGCC 
TCTCAGACCT GCATGACCAA GAAAGGATTT ACCCCTCTCC ACGTTGCAGC CAAGTATGGG 
AAGGTGGATG TGGCAGAGCT GCTGCTGGTG CATGATGCTC ACCCCAATGC AGCGGGGAAG 
AACGGCCTGA CCCCACTGCA CGTAGCTGTG CACCACAACA ACCTGGAGAT CGTCAAGCTT 
CTGCTTCCCA AGGGGAGCTC TCCACACAGC TCAGCCTGGA ACGGGTACAC CCCTCTGCAC 
ATTGCCGCCA AGCAGAACCA GATGGAGGTG GCCAGCAGCT TGCTGCAGTA TGGTGCTTCT 
GCAAATGCGG AGTCTGTTCA GGGAGTCACC CCTCTGCACC TGGCTTCTCA GGAAGGGCAT 
ACAGACATGG TAGCACTGCT TTTCTCCAAG CAAGCCAATG GAAACTTAGG CAACAAGAGT 
GGTCTGACTC CTCTCCATCT CGTGGCCCAA GAGGGACACG TACCAGTGGC TGATGTTCTG 
GTGAAACATG GAGTCACAGT GGATGCGACA ACCAGGATGG GCTACACCTC ACTGCACATA 
GCCAGCCACT ATGGGAACAT CAAGTTGGTG AAGTTTCTGC TGCAGCACCA GGCTGATGTC 
AATGCTAAGA CTAAGCTGGG CTACACACCC CTGCACCAGG CGGCACAGCA GGGCCACACG 
GATGTGGTGA CCCTGCTGCT GAAGCATGGT GCCTCTCCCA ACGAGATCAG CACAAATGGC 
ACCACTCCCC TGGCCATTGC GAAGCGCCTC GGTTACATTT CCGTCACAGA TGTGCTCAAG 
ATAGTCACAG AGGAGACCAC CATCCCACCA TTCAGTGACA AGTACCGCAT GAGCTTCCCG 
GAGACCGTGG ATGAGATTCT GGATGTGTCG GAGGATGAAG GCACTGCTCA TGTCACAGTA 
ATGGAGGAGG AGCTGGTCCC ACCGAAACCC AGAACACCTG AACCCAAGGA GCAGGAGGGC 
AAGAGGGAGA TGCTGGAGTT TGTGACCGTG ACAACACTGG AGCAAATGGA GGAATCTGCA 
GCTGTCCCAC AGGTCCCCTG CGTGCCCCCT GAGACTGTGG TGACCATAGC AGAGGAGTCT 
GAGCAGATGG GACCTGTGGA GACTGAGGCT GAGCAAGGCG GCCTGCTGCC TGCACCCTCG 
GTGTCCCCAC AGGAGCCCTC CAAGGAGTTC GATGAGGACT CCTTGATTCC CAGCAGCCCG 
GCCACCGAAA CCTCGGACAA CATCAGCCCC GTGGCCAGCC CCGTTCACAC AGGGTTCCTG 
GTGAGCTTCA TGGTGGATGC GCGTGGCGGC TCCATGCGGG GCAGCCGGCA CCACGGGCTG 
CGCGTGGTCA TCCCTCCCCG TGCCTGTGCT GCACCCACCC GCATCACTTG CCGCCTGGTG 
AAGCCTCAGA AGCTGCCTGC CCCCCCACCG CTGGCTGAGG AGGAGGGCCT GGCAAGCCGC 
ATCATTGCAC TGGGGCCCGC TGGCGCCCAG TTCCTCAGCC CCGTCATTGT GGAGATTCCT 
CACTTTGCCT CCTATGGACG TGGGGACCGT GAGCTGGTGG TGTTGCGCAG TGAGAATGGC 
TCTGTCTGGA AGGAGCACCG CAACCGCTAC GAGGAGAGCT ACATGGACCA ACTGCTCAGT 
GGCATGGATG AGGAGCTGGA GAGCCAAGAG GAGCTGGAGA AGAAGAGGGT CTGCCGCATC 
ATCACCACTG ACTTTCCTCT CTACTTCGTG GTCATGTCCC GGATTTGCCA GGACTGTGAC 
ATGATTGGCC CCGAGGGAGG GTGCTTGAAA AGCACACTGG TGCCCATGGT GCAGGCCACC 
TTCCCAGACA CCGCTGTCAC CAAGAAAGTG AGGCTGGCTC TGCAGGCGCA GCCCGTGCCT 
GATGAGCTGG TGACCAAGCT GCTGGGGAAC CAGGCAACCT TCAGCCCCAT CGTCACAGTG 
GAGCCACGTC GGAGGAAGTT CCACCGCCCC ATTGGCCTCC GCATCCCTCT GCCCCCATCC 
TGGAAGGACA ATCCCCGGGA CAGCGGCGAG GGTGACACCA CCAGCCTGCG CCTGCTCTGT 
TCTGTGATAG GAGGGACAGC ACAAGCTCAG TGGGAAGACA TCACAGGCAC CACAAAGCTG 
GTCTATGAAA AAGAGTGTGC TAACTTTACC ACCAATGTGT CTGCCAGGTT CTGGCTGGCT 
GACTGCCCGC GCACTGCTGA GGCTGTGCAC TTTGCCACCG TGCTGTACAA GGAACTGACG 
GCTGTGCCCT ACATGGCCAA ATTTGTGGTG TTTGCTAAGA TGAACGATGG ACGGGAAGGC 
CGGCTGCGCT GCTACTGCAT GACTGATGAC AAGGTGGACA AGACTTTGGA GCATCATGAG 
AACTTCACTG AGGTGGCCCG CAGCAGGGAC ATTGAGGTGG TGGAAGGAAT GCCTCTGCAT 
GTCGAGCTCT CAGGGAACCT GGTGCCCGTC AAGAAGGCCA CGCAGTCACG CACCTTCTTC 
TTCCAGTCCT TCCGGGAAAG CCGCCTCGCC ATCCCCATCA AGGTCCGGGA CAGCAGCCGG 
GAAGCCAGTG GCTCCCTGTC CTTCCTGCGC AAGGCCATGA AGTATGAGGA CTTGCAGCAT 
GTGCTCTGCC ACCTCAACAT CAGCATTCCA CCCTGCACCA AGGGAAGCAG CACTGAGGAG 
CGAAGGAGGA CACTGACACC TTTGTCTCTG CGCTCTCTGA GCAGCACCGA CAGGGCAGAC 
CAGAAGATGA TCGACATTGC AGAGCAGCTT GGCCTCAGCT GGGCAGAGCT GGCACGTGAG 
CTGCAGTTTG GGGTGGATGA CATCAACAGG ATTCGTGTGG AGAACCCTAA CTCCTTGCTG 
GAGCAGAGCA TAGCCTTGCT TAACCTCTGG GTCAGCCGCG AGGGCAAGGG TGTCAAAATT 
GAGAACCTGT ACACAGCGCT GAGGAACATT GACCGCAGCG AGATCATCAG CACGTTGGAG 
GGCTCCGGCC GGCAGAGCCG CAGCATGAAG GGCAGCTGGC GTTACATGGA CAGGGACTAT 
TCACTCTCGC CCTCCCAGAT GAATGGTTAC GCTTCGCTGC AGGACGAGCT GCTCTCCCCT 
GCCTCCCTGC ATTATATGCT GCCATCCCCT CTGCGTGCTG ACCAGTACTG GAACGAGGTG 
GCCATCATGG ACGCCATCCC CATGGCCGCC ACTGAGCAGG ACGCCCTGAT GGAGATGTCT 
GACATGCAGG TGTGGTCCTC AGGGCTCACC CCTTCACTGG TGACGGCTGA GGACTCCTCT 
CTGGAGTGCA GCAAGGCTGA GGACTCGGAT GCTACAAGCG AAGGTCGTTT CCCAGGGCAC 
CTTCTGACAG ATGCACATGT CCCAGACCAC ATGGGCTCTA TGGACTTAGT TGAGGATGAC 
ACAGTGGACT CAGACGCCAT GAACGGGCTG ATTGAACTGC TAGAGCAGGA GGAGGGGCAG 
AGGCCAGAGG GGAAGATGCC AGCCAGCGAC CACCAGCTAG GGACTGGAGA GCAGAACCCA 
GAGAGTGAAG TCTCTTTTGT TTCAGTTCAA CAGAAGGTAC AAGCCACGAT CGTGCCATCG 
CCAACCGTGA GCCACACAGT GGAGAGGAGT GCAGACAGGC TGAGGGACTG GAATGCAGAA 
GGCTCCTTTA TCTCCTGCCT ACAGGACCTG ACAGAGGGCT CCTGGCAGGA GGGGGTCACC 
CGACGGCTGG GTCCAGCACA CACCGTGGCT GCTGGGGCGC AGGGCCAGGA GCAAGAGCAG 
GTCCTGGCGC CGGACGTGGA GCTGATGCGG GTCAGCTCCA CAGAGGACAG CGACTGGCAG 
CCCCAGCACC CCGCGGGCGG CTGGCCAGAG GAGCCTGACA GCCGCTGCTT TGGGCAGGGC 
AACGAAGTCC TCCATCTCCC TGGGGAGCAT GTGACTGAGG AGCAGTTCAC AGATGAACAA 
GGCAATATAA TCACCAAGAA GGTCATCCGG AAAGTGGTGC ATCAGTTGGA CCTCGACACG 
GATGACAGGA AGGAGCATGA GGACTTGATT CTGGAGGGCT CCCTGCAGGA GCCCCAAGAC 
CTGGAGGCAG AGGCTGATCA CTTCATGTAC TCCATCCTAC AGCGGGACGG TCTGGGGGCC 
AAGGAGGAGG TGCAAGTGCG TGTCCCAAAA CTGGAAGTCT CCGGGAGCAG GATGGGGGCT 
CAGATAGTGA AACGAGCCAG TCTGAAAAGG GGGAAGCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152879.1
CDS	871..6732
Translation	MAQAAKQLKKIKDIEAQALQEQKEKEESNRKRRNRSRDRKKKADAATSFLRAARSGNLDRALDHLRNGVDINTCNQNGLNALHLASKEGHTKMVVELLHKEIVLETTTKKGNTALHIAALAGQQDVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLSVAQLLLNRGASVNFTPQNGITPLHIASRRGNIIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRIAEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYSAEIDDITLDHLTPLHVAAHCGHHRVAKLLVEKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVAAFMGHLPIVKTLLQRGASPNVSNVKVETPLHMAARAGHMDVAKYLIQNKAKINAKAKDDQTPLHCAARIGHTSMVQLLLENNADPNLATTAGHTPLHIAAREGHVDTALALLEKGASQTCMTKKGFTPLHVAAKYGKVDVAELLLVHDAHPNAAGKNGLTPLHVAVHHNNLEIVKLLLPKGSSPHSSAWNGYTPLHIAAKQNQMEVASSLLQYGASANAESVQGVTPLHLASQEGHTDMVALLFSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLVKHGVTVDATTRMGYTSLHIASHYGNIKLVKFLLQHQADVNAKTKLGYTPLHQAAQQGHTDVVTLLLKHGASPNEISTNGTTPLAIAKRLGYISVTDVLKIVTEETTIPPFSDKYRMSFPETVDEILDVSEDEGTAHVTVMEEELVPPKPRTPEPKEQEGKREMLEFVTVTTLEQMEESAAVPQVPCVPPETVVTIAEESEQMGPVETEAEQGGLLPAPSVSPQEPSKEFDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHHGLRVVIPPRACAAPTRITCRLVKPQKLPAPPPLAEEEGLASRIIALGPAGAQFLSPVIVEIPHFASYGRGDRELVVLRSENGSVWKEHRNRYEESYMDQLLSGMDEELESQEELEKKRVCRIITTDFPLYFVVMSRICQDCDMIGPEGGCLKSTLVPMVQATFPDTAVTKKVRLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWKDNPRDSGEGDTTSLRLLCSVIGGTAQAQWEDITGTTKLVYEKECANFTTNVSARFWLADCPRTAEAVHFATVLYKELTAVPYMAKFVVFAKMNDGREGRLRCYCMTDDKVDKTLEHHENFTEVARSRDIEVVEGMPLHVELSGNLVPVKKATQSRTFFFQSFRESRLAIPIKVRDSSREASGSLSFLRKAMKYEDLQHVLCHLNISIPPCTKGSSTEERRRTLTPLSLRSLSSTDRADQKMIDIAEQLGLSWAELARELQFGVDDINRIRVENPNSLLEQSIALLNLWVSREGKGVKIENLYTALRNIDRSEIISTLEGSGRQSRSMKGSWRYMDRDYSLSPSQMNGYASLQDELLSPASLHYMLPSPLRADQYWNEVAIMDAIPMAATEQDALMEMSDMQVWSSGLTPSLVTAEDSSLECSKAEDSDATSEGRFPGHLLTDAHVPDHMGSMDLVEDDTVDSDAMNGLIELLEQEEGQRPEGKMPASDHQLGTGEQNPESEVSFVSVQQKVQATIVPSPTVSHTVERSADRLRDWNAEGSFISCLQDLTEGSWQEGVTRRLGPAHTVAAGAQGQEQEQVLAPDVELMRVSSTEDSDWQPQHPAGGWPEEPDSRCFGQGNEVLHLPGEHVTEEQFTDEQGNIITKKVIRKVVHQLDLDTDDRKEHEDLILEGSLQEPQDLEAEADHFMYSILQRDGLGAKEEVQVRVPKLEVSGSRMGAQIVKRASLKRGKH

Target ORF information:

RefSeq Version	XM_015297393.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297393.1

ORF Insert Sequence:

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGGCTG ATGCTGCGAC CAGCTTCCTG AGAGCTGCAA GATCTGGGAA TCTGGACAGA 
GCCTTGGATC ATCTCAGGAA TGGGGTAGAT ATTAACACCT GTAACCAGAA TGGGCTGAAT 
GCCTTGCACC TGGCCTCCAA GGAGGGCCAC ACGAAAATGG TGGTGGAGCT TCTGCACAAG 
GAGATTGTTT TGGAGACCAC TACCAAGAAG GGAAACACAG CCCTGCACAT CGCTGCCCTC 
GCTGGACAGC AGGACGTGGT CCGGGAACTG GTGAACTATG GGGCCAATGT CAATGCTCAG 
TCGCAGAAGG GCTTCACACC ACTCTACATG GCAGCGCAGG AAAACCACCT GGAAGTTGTC 
AAATTCCTGC TGGAAAACGG AGCCAACCAG AATGTAGCCA CAGAGGATGG CTTCACACCA 
CTAGCTGTGG CTCTGCAGCA GGGTCATGAG AATGTGGTTG CTCACCTTAT CAACTATGGG 
ACGAAGGGTA AGGTCCGCCT GCCTGCCTTG CACATTGCAG CTCGCAACGA TGACACCCGT 
ACAGCCGCCG TGCTGCTGCA GAATGACCCC AATGCTGACG TCCTCTCCAA GACTGGATTT 
ACACCTTTGC ACATTGCAGC CCACTATGAG AATCTCAGCG TGGCCCAATT ATTGCTGAAT 
CGTGGAGCCA GCGTCAACTT CACACCCCAG AATGGGATCA CTCCTTTGCA CATAGCCTCT 
CGTCGAGGCA ACATCATCAT GGTACGGCTG CTGCTGGACC GTGGGGCCCA GATAGAGACA 
AGGACCAAGG ATGAGTTGAC GCCTCTCCAC TGTGCAGCTC GCAATGGACA TGTGCGAATT 
GCAGAGATCC TACTGGACCA TGGTGCTCCC ATTCAAGCCA AAACAAAGAA CGGTCTGTCA 
CCGATCCACA TGGCAGCACA GGGTGACCAC CTGGACTGTG TGCGCCTACT CCTGCAGTAC 
AGTGCTGAGA TAGACGACAT CACCCTGGAC CACCTGACAC CACTGCATGT AGCTGCGCAC 
TGTGGCCACC ACCGTGTGGC CAAGCTGCTG GTGGAGAAGG GAGCCAAGCC CAACTCCAGA 
GCTCTGAATG GCTTCACGCC TCTCCACATT GCCTGCAAGA AGAACCACAT TCGGGTGATG 
GAGTTGCTTC TGAAGACAGG AGCCTCCATT GATGCTGTCA CAGAGTCTGG CCTGACCCCC 
TTGCATGTGG CTGCCTTCAT GGGGCACTTG CCCATTGTCA AAACCTTGCT GCAGCGTGGA 
GCATCTCCTA ATGTGTCCAA TGTGAAAGTG GAGACTCCCC TGCACATGGC AGCCAGAGCT 
GGGCACATGG ATGTGGCCAA GTATCTGATA CAGAACAAGG CCAAAATCAA CGCCAAGGCC 
AAGGATGACC AGACTCCTCT GCACTGTGCT GCACGCATTG GCCACACCAG CATGGTCCAA 
CTCCTGCTGG AGAACAATGC CGACCCCAAC CTGGCCACTA CAGCAGGGCA CACGCCTCTG 
CACATCGCTG CCAGAGAGGG GCATGTAGAC ACAGCCTTGG CCCTGCTAGA GAAGGGAGCC 
TCTCAGACCT GCATGACCAA GAAAGGATTT ACCCCTCTCC ACGTTGCAGC CAAGTATGGG 
AAGGTGGATG TGGCAGAGCT GCTGCTGGTG CATGATGCTC ACCCCAATGC AGCGGGGAAG 
AACGGCCTGA CCCCACTGCA CGTAGCTGTG CACCACAACA ACCTGGAGAT CGTCAAGCTT 
CTGCTTCCCA AGGGGAGCTC TCCACACAGC TCAGCCTGGA ACGGGTACAC CCCTCTGCAC 
ATTGCCGCCA AGCAGAACCA GATGGAGGTG GCCAGCAGCT TGCTGCAGTA TGGTGCTTCT 
GCAAATGCGG AGTCTGTTCA GGGAGTCACC CCTCTGCACC TGGCTTCTCA GGAAGGGCAT 
ACAGACATGG TAGCACTGCT TTTCTCCAAG CAAGCCAATG GAAACTTAGG CAACAAGAGT 
GGTCTGACTC CTCTCCATCT CGTGGCCCAA GAGGGACACG TACCAGTGGC TGATGTTCTG 
GTGAAACATG GAGTCACAGT GGATGCGACA ACCAGGATGG GCTACACCTC ACTGCACATA 
GCCAGCCACT ATGGGAACAT CAAGTTGGTG AAGTTTCTGC TGCAGCACCA GGCTGATGTC 
AATGCTAAGA CTAAGCTGGG CTACACACCC CTGCACCAGG CGGCACAGCA GGGCCACACG 
GATGTGGTGA CCCTGCTGCT GAAGCATGGT GCCTCTCCCA ACGAGATCAG CACAAATGGC 
ACCACTCCCC TGGCCATTGC GAAGCGCCTC GGTTACATTT CCGTCACAGA TGTGCTCAAG 
ATAGTCACAG AGGAGACCAC CATCCCACCA TTCAGTGACA AGTACCGCAT GAGCTTCCCG 
GAGACCGTGG ATGAGATTCT GGATGTGTCG GAGGATGAAG GCACTGCTCA TGTCACAGTA 
ATGGAGGAGG AGCTGGTCCC ACCGAAACCC AGAACACCTG AACCCAAGGA GCAGGAGGGC 
AAGAGGGAGA TGCTGGAGTT TGTGACCGTG ACAACACTGG AGCAAATGGA GGAATCTGCA 
GCTGTCCCAC AGGTCCCCTG CGTGCCCCCT GAGACTGTGG TGACCATAGC AGAGGAGTCT 
GAGCAGATGG GACCTGTGGA GACTGAGGCT GAGCAAGGCG GCCTGCTGCC TGCACCCTCG 
GTGTCCCCAC AGGAGCCCTC CAAGGAGTTC GATGAGGACT CCTTGATTCC CAGCAGCCCG 
GCCACCGAAA CCTCGGACAA CATCAGCCCC GTGGCCAGCC CCGTTCACAC AGGGTTCCTG 
GTGAGCTTCA TGGTGGATGC GCGTGGCGGC TCCATGCGGG GCAGCCGGCA CCACGGGCTG 
CGCGTGGTCA TCCCTCCCCG TGCCTGTGCT GCACCCACCC GCATCACTTG CCGCCTGGTG 
AAGCCTCAGA AGCTGCCTGC CCCCCCACCG CTGGCTGAGG AGGAGGGCCT GGCAAGCCGC 
ATCATTGCAC TGGGGCCCGC TGGCGCCCAG TTCCTCAGCC CCGTCATTGT GGAGATTCCT 
CACTTTGCCT CCTATGGACG TGGGGACCGT GAGCTGGTGG TGTTGCGCAG TGAGAATGGC 
TCTGTCTGGA AGGAGCACCG CAACCGCTAC GAGGAGAGCT ACATGGACCA ACTGCTCAGT 
GGCATGGATG AGGAGCTGGA GAGCCAAGAG GAGCTGGAGA AGAAGAGGGT CTGCCGCATC 
ATCACCACTG ACTTTCCTCT CTACTTCGTG GTCATGTCCC GGATTTGCCA GGACTGTGAC 
ATGATTGGCC CCGAGGGAGG GTGCTTGAAA AGCACACTGG TGCCCATGGT GCAGGCCACC 
TTCCCAGACA CCGCTGTCAC CAAGAAAGTG AGGCTGGCTC TGCAGGCGCA GCCCGTGCCT 
GATGAGCTGG TGACCAAGCT GCTGGGGAAC CAGGCAACCT TCAGCCCCAT CGTCACAGTG 
GAGCCACGTC GGAGGAAGTT CCACCGCCCC ATTGGCCTCC GCATCCCTCT GCCCCCATCC 
TGGAAGGACA ATCCCCGGGA CAGCGGCGAG GGTGACACCA CCAGCCTGCG CCTGCTCTGT 
TCTGTGATAG GAGGGACAGC ACAAGCTCAG TGGGAAGACA TCACAGGCAC CACAAAGCTG 
GTCTATGAAA AAGAGTGTGC TAACTTTACC ACCAATGTGT CTGCCAGGTT CTGGCTGGCT 
GACTGCCCGC GCACTGCTGA GGCTGTGCAC TTTGCCACCG TGCTGTACAA GGAACTGACG 
GCTGTGCCCT ACATGGCCAA ATTTGTGGTG TTTGCTAAGA TGAACGATGG ACGGGAAGGC 
CGGCTGCGCT GCTACTGCAT GACTGATGAC AAGGTGGACA AGACTTTGGA GCATCATGAG 
AACTTCACTG AGGTGGCCCG CAGCAGGGAC ATTGAGGTGG TGGAAGGAAT GCCTCTGCAT 
GTCGAGCTCT CAGGGAACCT GGTGCCCGTC AAGAAGGCCA CGCAGTCACG CACCTTCTTC 
TTCCAGTCCT TCCGGGAAAG CCGCCTCGCC ATCCCCATCA AGGTCCGGGA CAGCAGCCGG 
GAAGCCAGTG GCTCCCTGTC CTTCCTGCGC AAGGCCATGA AGTATGAGGA CTTGCAGCAT 
GTGCTCTGCC ACCTCAACAT CAGCATTCCA CCCTGCACCA AGGGAAGCAG CACTGAGGAG 
CGAAGGAGGA CACTGACACC TTTGTCTCTG CGCTCTCTGA GCAGCACCGA CAGGGCAGAC 
CAGAAGATGA TCGACATTGC AGAGCAGCTT GGCCTCAGCT GGGCAGAGCT GGCACGTGAG 
CTGCAGTTTG GGGTGGATGA CATCAACAGG ATTCGTGTGG AGAACCCTAA CTCCTTGCTG 
GAGCAGAGCA TAGCCTTGCT TAACCTCTGG GTCAGCCGCG AGGGCAAGGG TGTCAAAATT 
GAGAACCTGT ACACAGCGCT GAGGAACATT GACCGCAGCG AGATCATCAG CACGTTGGAG 
GGCTCCGGCC GGCAGAGCCG CAGCATGAAG GGCAGCTGGC GTTACATGGA CAGGGACTAT 
TCACTCTCGC CCTCCCAGAT GAATGGTTAC GCTTCGCTGC AGGACGAGCT GCTCTCCCCT 
GCCTCCCTGC ATTATATGCT GCCATCCCCT CTGCGTGCTG ACCAGTACTG GAACGAGGTG 
GCCATCATGG ACGCCATCCC CATGGCCGCC ACTGAGCAGG ACGCCCTGAT GGAGATGTCT 
GACATGCAGG TGTGGTCCTC AGGGCTCACC CCTTCACTGG TGACGGCTGA GGACTCCTCT 
CTGGAGTGCA GCAAGGCTGA GGACTCGGAT GCTACAAGCG AAGGTCGTTT CCCAGGGCAC 
CTTCTGACAG ATGCACATGT CCCAGACCAC ATGGGCTCTA TGGACTTAGT TGAGGATGAC 
ACAGTGGACT CAGACGCCAT GAACGGGCTG ATTGAACTGC TAGAGCAGGA GGAGGGGCAG 
AGGCCAGAGG GGAAGATGCC AGCCAGCGAC CACCAGCTAG GGACTGGAGA GCAGAACCCA 
GAGAGTGAAG TCTCTTTTGT TTCAGTTCAA CAGAAGGTAC AAGCCACGAT CGTGCCATCG 
CCAACCGTGA GCCACACAGT GGAGAGGAGT GCAGACAGGC TGAGGGACTG GAATGCAGAA 
GGCTCCTTTA TCTCCTGCCT ACAGGACCTG ACAGAGGGCT CCTGGCAGGA GGGGGTCACC 
CGACGGCTGG GTCCAGCACA CACCGTGGCT GCTGGGGCGC AGGGCCAGGA GCAAGAGCAG 
GTCCTGGCGC CGGACGTGGA GCTGATGCGG GTCAGCTCCA CAGAGGACAG CGACTGGCAG 
CCCCAGCACC CCGCGGGCGG CTGGCCAGAG GAGCCTGACA GCCGCTGCTT TGGGCAGGGC 
AACGAAGTCC TCCATCTCCC TGGGGAGCAT GTGACTGAGG AGCAGTTCAC AGATGAACAA 
GGCAATATAA TCACCAAGAA GGTCATCCGG AAAGTGGTGC ATCAGTTGGA CCTCGACACG 
GATGACAGGA AGGAGCATGA GGACTTGATT CTGGAGGGCT CCCTGCAGGA GCCCCAAGAC 
CTGGAGGCAG AGGCTGATCA CTTCATGTAC TCCATCCTAC AGCGGGACGG TCTGGGGGCC 
AAGGAGGAGG TGCAAGTGCG TGTCCCAAAA CTGGAAGTCT CCGGGAGCAG GATGGGGGCT 
CAGATAGTGA AACGAGCCAG TCTGAAAAGG GGGAAGCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK1 ( XM_015297394.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152880.1 Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X5, mRNA.

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CloneID	OGa45389
Clone ID Related Accession (Same CDS sequence)	XM_015297394.1
Accession Version	XM_015297394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ankyrin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455999.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGGGT TCAGTCCTGC TGATAACACT TTCAGTCTGG CTGCTCACAC TTTGTTTGGC 
TGCAGCATTC AGCATCAGTT TGCTGATCAG ACCTGCAGAT CCTGGCTGCC CGGGGCTGAT 
GCTGCGACCA GCTTCCTGAG AGCTGCAAGA TCTGGGAATC TGGACAGAGC CTTGGATCAT 
CTCAGGAATG GGGTAGATAT TAACACCTGT AACCAGAATG GGCTGAATGC CTTGCACCTG 
GCCTCCAAGG AGGGCCACAC GAAAATGGTG GTGGAGCTTC TGCACAAGGA GATTGTTTTG 
GAGACCACTA CCAAGAAGGG AAACACAGCC CTGCACATCG CTGCCCTCGC TGGACAGCAG 
GACGTGGTCC GGGAACTGGT GAACTATGGG GCCAATGTCA ATGCTCAGTC GCAGAAGGGC 
TTCACACCAC TCTACATGGC AGCGCAGGAA AACCACCTGG AAGTTGTCAA ATTCCTGCTG 
GAAAACGGAG CCAACCAGAA TGTAGCCACA GAGGATGGCT TCACACCACT AGCTGTGGCT 
CTGCAGCAGG GTCATGAGAA TGTGGTTGCT CACCTTATCA ACTATGGGAC GAAGGGTAAG 
GTCCGCCTGC CTGCCTTGCA CATTGCAGCT CGCAACGATG ACACCCGTAC AGCCGCCGTG 
CTGCTGCAGA ATGACCCCAA TGCTGACGTC CTCTCCAAGA CTGGATTTAC ACCTTTGCAC 
ATTGCAGCCC ACTATGAGAA TCTCAGCGTG GCCCAATTAT TGCTGAATCG TGGAGCCAGC 
GTCAACTTCA CACCCCAGAA TGGGATCACT CCTTTGCACA TAGCCTCTCG TCGAGGCAAC 
ATCATCATGG TACGGCTGCT GCTGGACCGT GGGGCCCAGA TAGAGACAAG GACCAAGGAT 
GAGTTGACGC CTCTCCACTG TGCAGCTCGC AATGGACATG TGCGAATTGC AGAGATCCTA 
CTGGACCATG GTGCTCCCAT TCAAGCCAAA ACAAAGAACG GTCTGTCACC GATCCACATG 
GCAGCACAGG GTGACCACCT GGACTGTGTG CGCCTACTCC TGCAGTACAG TGCTGAGATA 
GACGACATCA CCCTGGACCA CCTGACACCA CTGCATGTAG CTGCGCACTG TGGCCACCAC 
CGTGTGGCCA AGCTGCTGGT GGAGAAGGGA GCCAAGCCCA ACTCCAGAGC TCTGAATGGC 
TTCACGCCTC TCCACATTGC CTGCAAGAAG AACCACATTC GGGTGATGGA GTTGCTTCTG 
AAGACAGGAG CCTCCATTGA TGCTGTCACA GAGTCTGGCC TGACCCCCTT GCATGTGGCT 
GCCTTCATGG GGCACTTGCC CATTGTCAAA ACCTTGCTGC AGCGTGGAGC ATCTCCTAAT 
GTGTCCAATG TGAAAGTGGA GACTCCCCTG CACATGGCAG CCAGAGCTGG GCACATGGAT 
GTGGCCAAGT ATCTGATACA GAACAAGGCC AAAATCAACG CCAAGGCCAA GGATGACCAG 
ACTCCTCTGC ACTGTGCTGC ACGCATTGGC CACACCAGCA TGGTCCAACT CCTGCTGGAG 
AACAATGCCG ACCCCAACCT GGCCACTACA GCAGGGCACA CGCCTCTGCA CATCGCTGCC 
AGAGAGGGGC ATGTAGACAC AGCCTTGGCC CTGCTAGAGA AGGGAGCCTC TCAGACCTGC 
ATGACCAAGA AAGGATTTAC CCCTCTCCAC GTTGCAGCCA AGTATGGGAA GGTGGATGTG 
GCAGAGCTGC TGCTGGTGCA TGATGCTCAC CCCAATGCAG CGGGGAAGAA CGGCCTGACC 
CCACTGCACG TAGCTGTGCA CCACAACAAC CTGGAGATCG TCAAGCTTCT GCTTCCCAAG 
GGGAGCTCTC CACACAGCTC AGCCTGGAAC GGGTACACCC CTCTGCACAT TGCCGCCAAG 
CAGAACCAGA TGGAGGTGGC CAGCAGCTTG CTGCAGTATG GTGCTTCTGC AAATGCGGAG 
TCTGTTCAGG GAGTCACCCC TCTGCACCTG GCTTCTCAGG AAGGGCATAC AGACATGGTA 
GCACTGCTTT TCTCCAAGCA AGCCAATGGA AACTTAGGCA ACAAGAGTGG TCTGACTCCT 
CTCCATCTCG TGGCCCAAGA GGGACACGTA CCAGTGGCTG ATGTTCTGGT GAAACATGGA 
GTCACAGTGG ATGCGACAAC CAGGATGGGC TACACCTCAC TGCACATAGC CAGCCACTAT 
GGGAACATCA AGTTGGTGAA GTTTCTGCTG CAGCACCAGG CTGATGTCAA TGCTAAGACT 
AAGCTGGGCT ACACACCCCT GCACCAGGCG GCACAGCAGG GCCACACGGA TGTGGTGACC 
CTGCTGCTGA AGCATGGTGC CTCTCCCAAC GAGATCAGCA CAAATGGCAC CACTCCCCTG 
GCCATTGCGA AGCGCCTCGG TTACATTTCC GTCACAGATG TGCTCAAGAT AGTCACAGAG 
GAGACCACCA TCCCACCATT CAGTGACAAG TACCGCATGA GCTTCCCGGA GACCGTGGAT 
GAGATTCTGG ATGTGTCGGA GGATGAAGGC ACTGCTCATG TCACAGTAAT GGAGGAGGAG 
CTGGTCCCAC CGAAACCCAG AACACCTGAA CCCAAGGAGC AGGAGGGCAA GAGGGAGATG 
CTGGAGTTTG TGACCGTGAC AACACTGGAG CAAATGGAGG AATCTGCAGC TGTCCCACAG 
GTCCCCTGCG TGCCCCCTGA GACTGTGGTG ACCATAGCAG AGGAGTCTGA GCAGATGGGA 
CCTGTGGAGA CTGAGGCTGA GCAAGGCGGC CTGCTGCCTG CACCCTCGGT GTCCCCACAG 
GAGCCCTCCA AGGAGTTCGA TGAGGACTCC TTGATTCCCA GCAGCCCGGC CACCGAAACC 
TCGGACAACA TCAGCCCCGT GGCCAGCCCC GTTCACACAG GGTTCCTGGT GAGCTTCATG 
GTGGATGCGC GTGGCGGCTC CATGCGGGGC AGCCGGCACC ACGGGCTGCG CGTGGTCATC 
CCTCCCCGTG CCTGTGCTGC ACCCACCCGC ATCACTTGCC GCCTGGTGAA GCCTCAGAAG 
CTGCCTGCCC CCCCACCGCT GGCTGAGGAG GAGGGCCTGG CAAGCCGCAT CATTGCACTG 
GGGCCCGCTG GCGCCCAGTT CCTCAGCCCC GTCATTGTGG AGATTCCTCA CTTTGCCTCC 
TATGGACGTG GGGACCGTGA GCTGGTGGTG TTGCGCAGTG AGAATGGCTC TGTCTGGAAG 
GAGCACCGCA ACCGCTACGA GGAGAGCTAC ATGGACCAAC TGCTCAGTGG CATGGATGAG 
GAGCTGGAGA GCCAAGAGGA GCTGGAGAAG AAGAGGGTCT GCCGCATCAT CACCACTGAC 
TTTCCTCTCT ACTTCGTGGT CATGTCCCGG ATTTGCCAGG ACTGTGACAT GATTGGCCCC 
GAGGGAGGGT GCTTGAAAAG CACACTGGTG CCCATGGTGC AGGCCACCTT CCCAGACACC 
GCTGTCACCA AGAAAGTGAG GCTGGCTCTG CAGGCGCAGC CCGTGCCTGA TGAGCTGGTG 
ACCAAGCTGC TGGGGAACCA GGCAACCTTC AGCCCCATCG TCACAGTGGA GCCACGTCGG 
AGGAAGTTCC ACCGCCCCAT TGGCCTCCGC ATCCCTCTGC CCCCATCCTG GAAGGACAAT 
CCCCGGGACA GCGGCGAGGG TGACACCACC AGCCTGCGCC TGCTCTGTTC TGTGATAGGA 
GGGACAGCAC AAGCTCAGTG GGAAGACATC ACAGGCACCA CAAAGCTGGT CTATGAAAAA 
GAGTGTGCTA ACTTTACCAC CAATGTGTCT GCCAGGTTCT GGCTGGCTGA CTGCCCGCGC 
ACTGCTGAGG CTGTGCACTT TGCCACCGTG CTGTACAAGG AACTGACGGC TGTGCCCTAC 
ATGGCCAAAT TTGTGGTGTT TGCTAAGATG AACGATGGAC GGGAAGGCCG GCTGCGCTGC 
TACTGCATGA CTGATGACAA GGTGGACAAG ACTTTGGAGC ATCATGAGAA CTTCACTGAG 
GTGGCCCGCA GCAGGGACAT TGAGGTGGTG GAAGGAATGC CTCTGCATGT CGAGCTCTCA 
GGGAACCTGG TGCCCGTCAA GAAGGCCACG CAGTCACGCA CCTTCTTCTT CCAGTCCTTC 
CGGGAAAGCC GCCTCGCCAT CCCCATCAAG GTCCGGGACA GCAGCCGGGA AGCCAGTGGC 
TCCCTGTCCT TCCTGCGCAA GGCCATGAAG TATGAGGACT TGCAGCATGT GCTCTGCCAC 
CTCAACATCA GCATTCCACC CTGCACCAAG GGAAGCAGCA CTGAGGAGCG AAGGAGGACA 
CTGACACCTT TGTCTCTGCG CTCTCTGAGC AGCACCGACA GGGCAGACCA GAAGATGATC 
GACATTGCAG AGCAGCTTGG CCTCAGCTGG GCAGAGCTGG CACGTGAGCT GCAGTTTGGG 
GTGGATGACA TCAACAGGAT TCGTGTGGAG AACCCTAACT CCTTGCTGGA GCAGAGCATA 
GCCTTGCTTA ACCTCTGGGT CAGCCGCGAG GGCAAGGGTG TCAAAATTGA GAACCTGTAC 
ACAGCGCTGA GGAACATTGA CCGCAGCGAG ATCATCAGCA CGTTGGAGGG CTCCGGCCGG 
CAGAGCCGCA GCATGAAGGG CAGCTGGCGT TACATGGACA GGGACTATTC ACTCTCGCCC 
TCCCAGATGA ATGGTTACGC TTCGCTGCAG GACGAGCTGC TCTCCCCTGC CTCCCTGCAT 
TATATGCTGC CATCCCCTCT GCGTGCTGAC CAGTACTGGA ACGAGGTGGC CATCATGGAC 
GCCATCCCCA TGGCCGCCAC TGAGCAGGAC GCCCTGATGG AGATGTCTGA CATGCAGGTG 
TGGTCCTCAG GGCTCACCCC TTCACTGGTG ACGGCTGAGG ACTCCTCTCT GGAGTGCAGC 
AAGGCTGAGG ACTCGGATGC TACAAGCGAA GGTCGTTTCC CAGGGCACCT TCTGACAGAT 
GCACATGTCC CAGACCACAT GGGCTCTATG GACTTAGTTG AGGATGACAC AGTGGACTCA 
GACGCCATGA ACGGGCTGAT TGAACTGCTA GAGCAGGAGG AGGGGCAGAG GCCAGAGGGG 
AAGATGCCAG CCAGCGACCA CCAGCTAGGG ACTGGAGAGC AGAACCCAGA GAGTGAAGTC 
TCTTTTGTTT CAGTTCAACA GAAGGTACAA GCCACGATCG TGCCATCGCC AACCGTGAGC 
CACACAGTGG AGAGGAGTGC AGACAGGCTG AGGGACTGGA ATGCAGAAGG CTCCTTTATC 
TCCTGCCTAC AGGACCTGAC AGAGGGCTCC TGGCAGGAGG GGGTCACCCG ACGGCTGGGT 
CCAGCACACA CCGTGGCTGC TGGGGCGCAG GGCCAGGAGC AAGAGCAGGT CCTGGCGCCG 
GACGTGGAGC TGATGCGGGT CAGCTCCACA GAGGACAGCG ACTGGCAGCC CCAGCACCCC 
GCGGGCGGCT GGCCAGAGGA GCCTGACAGC CGCTGCTTTG GGCAGGGCAA CGAAGTCCTC 
CATCTCCCTG GGGAGCATGT GACTGAGGAG CAGTTCACAG ATGAACAAGG CAATATAATC 
ACCAAGAAGG TCATCCGGAA AGTGGTGCAT CAGTTGGACC TCGACACGGA TGACAGGAAG 
GAGCATGAGG ACTTGATTCT GGAGGGCTCC CTGCAGGAGC CCCAAGACCT GGAGGCAGAG 
GCTGATCACT TCATGTACTC CATCCTACAG CGGGACGGTC TGGGGGCCAA GGAGGAGGTG 
CAAGTGCGTG TCCCAAAACT GGAAGTCTCC GGGAGCAGGA TGGGGGCTCA GATAGTGAAA 
CGAGCCAGTC TGAAAAGGGG GAAGCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152880.1
CDS	16..5865
Translation	MIGFSPADNTFSLAAHTLFGCSIQHQFADQTCRSWLPGADAATSFLRAARSGNLDRALDHLRNGVDINTCNQNGLNALHLASKEGHTKMVVELLHKEIVLETTTKKGNTALHIAALAGQQDVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLSVAQLLLNRGASVNFTPQNGITPLHIASRRGNIIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRIAEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYSAEIDDITLDHLTPLHVAAHCGHHRVAKLLVEKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVAAFMGHLPIVKTLLQRGASPNVSNVKVETPLHMAARAGHMDVAKYLIQNKAKINAKAKDDQTPLHCAARIGHTSMVQLLLENNADPNLATTAGHTPLHIAAREGHVDTALALLEKGASQTCMTKKGFTPLHVAAKYGKVDVAELLLVHDAHPNAAGKNGLTPLHVAVHHNNLEIVKLLLPKGSSPHSSAWNGYTPLHIAAKQNQMEVASSLLQYGASANAESVQGVTPLHLASQEGHTDMVALLFSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLVKHGVTVDATTRMGYTSLHIASHYGNIKLVKFLLQHQADVNAKTKLGYTPLHQAAQQGHTDVVTLLLKHGASPNEISTNGTTPLAIAKRLGYISVTDVLKIVTEETTIPPFSDKYRMSFPETVDEILDVSEDEGTAHVTVMEEELVPPKPRTPEPKEQEGKREMLEFVTVTTLEQMEESAAVPQVPCVPPETVVTIAEESEQMGPVETEAEQGGLLPAPSVSPQEPSKEFDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHHGLRVVIPPRACAAPTRITCRLVKPQKLPAPPPLAEEEGLASRIIALGPAGAQFLSPVIVEIPHFASYGRGDRELVVLRSENGSVWKEHRNRYEESYMDQLLSGMDEELESQEELEKKRVCRIITTDFPLYFVVMSRICQDCDMIGPEGGCLKSTLVPMVQATFPDTAVTKKVRLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWKDNPRDSGEGDTTSLRLLCSVIGGTAQAQWEDITGTTKLVYEKECANFTTNVSARFWLADCPRTAEAVHFATVLYKELTAVPYMAKFVVFAKMNDGREGRLRCYCMTDDKVDKTLEHHENFTEVARSRDIEVVEGMPLHVELSGNLVPVKKATQSRTFFFQSFRESRLAIPIKVRDSSREASGSLSFLRKAMKYEDLQHVLCHLNISIPPCTKGSSTEERRRTLTPLSLRSLSSTDRADQKMIDIAEQLGLSWAELARELQFGVDDINRIRVENPNSLLEQSIALLNLWVSREGKGVKIENLYTALRNIDRSEIISTLEGSGRQSRSMKGSWRYMDRDYSLSPSQMNGYASLQDELLSPASLHYMLPSPLRADQYWNEVAIMDAIPMAATEQDALMEMSDMQVWSSGLTPSLVTAEDSSLECSKAEDSDATSEGRFPGHLLTDAHVPDHMGSMDLVEDDTVDSDAMNGLIELLEQEEGQRPEGKMPASDHQLGTGEQNPESEVSFVSVQQKVQATIVPSPTVSHTVERSADRLRDWNAEGSFISCLQDLTEGSWQEGVTRRLGPAHTVAAGAQGQEQEQVLAPDVELMRVSSTEDSDWQPQHPAGGWPEEPDSRCFGQGNEVLHLPGEHVTEEQFTDEQGNIITKKVIRKVVHQLDLDTDDRKEHEDLILEGSLQEPQDLEAEADHFMYSILQRDGLGAKEEVQVRVPKLEVSGSRMGAQIVKRASLKRGKH

Target ORF information:

RefSeq Version	XM_015297394.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297394.1

ORF Insert Sequence:

ATGATAGGGT TCAGTCCTGC TGATAACACT TTCAGTCTGG CTGCTCACAC TTTGTTTGGC 
TGCAGCATTC AGCATCAGTT TGCTGATCAG ACCTGCAGAT CCTGGCTGCC CGGGGCTGAT 
GCTGCGACCA GCTTCCTGAG AGCTGCAAGA TCTGGGAATC TGGACAGAGC CTTGGATCAT 
CTCAGGAATG GGGTAGATAT TAACACCTGT AACCAGAATG GGCTGAATGC CTTGCACCTG 
GCCTCCAAGG AGGGCCACAC GAAAATGGTG GTGGAGCTTC TGCACAAGGA GATTGTTTTG 
GAGACCACTA CCAAGAAGGG AAACACAGCC CTGCACATCG CTGCCCTCGC TGGACAGCAG 
GACGTGGTCC GGGAACTGGT GAACTATGGG GCCAATGTCA ATGCTCAGTC GCAGAAGGGC 
TTCACACCAC TCTACATGGC AGCGCAGGAA AACCACCTGG AAGTTGTCAA ATTCCTGCTG 
GAAAACGGAG CCAACCAGAA TGTAGCCACA GAGGATGGCT TCACACCACT AGCTGTGGCT 
CTGCAGCAGG GTCATGAGAA TGTGGTTGCT CACCTTATCA ACTATGGGAC GAAGGGTAAG 
GTCCGCCTGC CTGCCTTGCA CATTGCAGCT CGCAACGATG ACACCCGTAC AGCCGCCGTG 
CTGCTGCAGA ATGACCCCAA TGCTGACGTC CTCTCCAAGA CTGGATTTAC ACCTTTGCAC 
ATTGCAGCCC ACTATGAGAA TCTCAGCGTG GCCCAATTAT TGCTGAATCG TGGAGCCAGC 
GTCAACTTCA CACCCCAGAA TGGGATCACT CCTTTGCACA TAGCCTCTCG TCGAGGCAAC 
ATCATCATGG TACGGCTGCT GCTGGACCGT GGGGCCCAGA TAGAGACAAG GACCAAGGAT 
GAGTTGACGC CTCTCCACTG TGCAGCTCGC AATGGACATG TGCGAATTGC AGAGATCCTA 
CTGGACCATG GTGCTCCCAT TCAAGCCAAA ACAAAGAACG GTCTGTCACC GATCCACATG 
GCAGCACAGG GTGACCACCT GGACTGTGTG CGCCTACTCC TGCAGTACAG TGCTGAGATA 
GACGACATCA CCCTGGACCA CCTGACACCA CTGCATGTAG CTGCGCACTG TGGCCACCAC 
CGTGTGGCCA AGCTGCTGGT GGAGAAGGGA GCCAAGCCCA ACTCCAGAGC TCTGAATGGC 
TTCACGCCTC TCCACATTGC CTGCAAGAAG AACCACATTC GGGTGATGGA GTTGCTTCTG 
AAGACAGGAG CCTCCATTGA TGCTGTCACA GAGTCTGGCC TGACCCCCTT GCATGTGGCT 
GCCTTCATGG GGCACTTGCC CATTGTCAAA ACCTTGCTGC AGCGTGGAGC ATCTCCTAAT 
GTGTCCAATG TGAAAGTGGA GACTCCCCTG CACATGGCAG CCAGAGCTGG GCACATGGAT 
GTGGCCAAGT ATCTGATACA GAACAAGGCC AAAATCAACG CCAAGGCCAA GGATGACCAG 
ACTCCTCTGC ACTGTGCTGC ACGCATTGGC CACACCAGCA TGGTCCAACT CCTGCTGGAG 
AACAATGCCG ACCCCAACCT GGCCACTACA GCAGGGCACA CGCCTCTGCA CATCGCTGCC 
AGAGAGGGGC ATGTAGACAC AGCCTTGGCC CTGCTAGAGA AGGGAGCCTC TCAGACCTGC 
ATGACCAAGA AAGGATTTAC CCCTCTCCAC GTTGCAGCCA AGTATGGGAA GGTGGATGTG 
GCAGAGCTGC TGCTGGTGCA TGATGCTCAC CCCAATGCAG CGGGGAAGAA CGGCCTGACC 
CCACTGCACG TAGCTGTGCA CCACAACAAC CTGGAGATCG TCAAGCTTCT GCTTCCCAAG 
GGGAGCTCTC CACACAGCTC AGCCTGGAAC GGGTACACCC CTCTGCACAT TGCCGCCAAG 
CAGAACCAGA TGGAGGTGGC CAGCAGCTTG CTGCAGTATG GTGCTTCTGC AAATGCGGAG 
TCTGTTCAGG GAGTCACCCC TCTGCACCTG GCTTCTCAGG AAGGGCATAC AGACATGGTA 
GCACTGCTTT TCTCCAAGCA AGCCAATGGA AACTTAGGCA ACAAGAGTGG TCTGACTCCT 
CTCCATCTCG TGGCCCAAGA GGGACACGTA CCAGTGGCTG ATGTTCTGGT GAAACATGGA 
GTCACAGTGG ATGCGACAAC CAGGATGGGC TACACCTCAC TGCACATAGC CAGCCACTAT 
GGGAACATCA AGTTGGTGAA GTTTCTGCTG CAGCACCAGG CTGATGTCAA TGCTAAGACT 
AAGCTGGGCT ACACACCCCT GCACCAGGCG GCACAGCAGG GCCACACGGA TGTGGTGACC 
CTGCTGCTGA AGCATGGTGC CTCTCCCAAC GAGATCAGCA CAAATGGCAC CACTCCCCTG 
GCCATTGCGA AGCGCCTCGG TTACATTTCC GTCACAGATG TGCTCAAGAT AGTCACAGAG 
GAGACCACCA TCCCACCATT CAGTGACAAG TACCGCATGA GCTTCCCGGA GACCGTGGAT 
GAGATTCTGG ATGTGTCGGA GGATGAAGGC ACTGCTCATG TCACAGTAAT GGAGGAGGAG 
CTGGTCCCAC CGAAACCCAG AACACCTGAA CCCAAGGAGC AGGAGGGCAA GAGGGAGATG 
CTGGAGTTTG TGACCGTGAC AACACTGGAG CAAATGGAGG AATCTGCAGC TGTCCCACAG 
GTCCCCTGCG TGCCCCCTGA GACTGTGGTG ACCATAGCAG AGGAGTCTGA GCAGATGGGA 
CCTGTGGAGA CTGAGGCTGA GCAAGGCGGC CTGCTGCCTG CACCCTCGGT GTCCCCACAG 
GAGCCCTCCA AGGAGTTCGA TGAGGACTCC TTGATTCCCA GCAGCCCGGC CACCGAAACC 
TCGGACAACA TCAGCCCCGT GGCCAGCCCC GTTCACACAG GGTTCCTGGT GAGCTTCATG 
GTGGATGCGC GTGGCGGCTC CATGCGGGGC AGCCGGCACC ACGGGCTGCG CGTGGTCATC 
CCTCCCCGTG CCTGTGCTGC ACCCACCCGC ATCACTTGCC GCCTGGTGAA GCCTCAGAAG 
CTGCCTGCCC CCCCACCGCT GGCTGAGGAG GAGGGCCTGG CAAGCCGCAT CATTGCACTG 
GGGCCCGCTG GCGCCCAGTT CCTCAGCCCC GTCATTGTGG AGATTCCTCA CTTTGCCTCC 
TATGGACGTG GGGACCGTGA GCTGGTGGTG TTGCGCAGTG AGAATGGCTC TGTCTGGAAG 
GAGCACCGCA ACCGCTACGA GGAGAGCTAC ATGGACCAAC TGCTCAGTGG CATGGATGAG 
GAGCTGGAGA GCCAAGAGGA GCTGGAGAAG AAGAGGGTCT GCCGCATCAT CACCACTGAC 
TTTCCTCTCT ACTTCGTGGT CATGTCCCGG ATTTGCCAGG ACTGTGACAT GATTGGCCCC 
GAGGGAGGGT GCTTGAAAAG CACACTGGTG CCCATGGTGC AGGCCACCTT CCCAGACACC 
GCTGTCACCA AGAAAGTGAG GCTGGCTCTG CAGGCGCAGC CCGTGCCTGA TGAGCTGGTG 
ACCAAGCTGC TGGGGAACCA GGCAACCTTC AGCCCCATCG TCACAGTGGA GCCACGTCGG 
AGGAAGTTCC ACCGCCCCAT TGGCCTCCGC ATCCCTCTGC CCCCATCCTG GAAGGACAAT 
CCCCGGGACA GCGGCGAGGG TGACACCACC AGCCTGCGCC TGCTCTGTTC TGTGATAGGA 
GGGACAGCAC AAGCTCAGTG GGAAGACATC ACAGGCACCA CAAAGCTGGT CTATGAAAAA 
GAGTGTGCTA ACTTTACCAC CAATGTGTCT GCCAGGTTCT GGCTGGCTGA CTGCCCGCGC 
ACTGCTGAGG CTGTGCACTT TGCCACCGTG CTGTACAAGG AACTGACGGC TGTGCCCTAC 
ATGGCCAAAT TTGTGGTGTT TGCTAAGATG AACGATGGAC GGGAAGGCCG GCTGCGCTGC 
TACTGCATGA CTGATGACAA GGTGGACAAG ACTTTGGAGC ATCATGAGAA CTTCACTGAG 
GTGGCCCGCA GCAGGGACAT TGAGGTGGTG GAAGGAATGC CTCTGCATGT CGAGCTCTCA 
GGGAACCTGG TGCCCGTCAA GAAGGCCACG CAGTCACGCA CCTTCTTCTT CCAGTCCTTC 
CGGGAAAGCC GCCTCGCCAT CCCCATCAAG GTCCGGGACA GCAGCCGGGA AGCCAGTGGC 
TCCCTGTCCT TCCTGCGCAA GGCCATGAAG TATGAGGACT TGCAGCATGT GCTCTGCCAC 
CTCAACATCA GCATTCCACC CTGCACCAAG GGAAGCAGCA CTGAGGAGCG AAGGAGGACA 
CTGACACCTT TGTCTCTGCG CTCTCTGAGC AGCACCGACA GGGCAGACCA GAAGATGATC 
GACATTGCAG AGCAGCTTGG CCTCAGCTGG GCAGAGCTGG CACGTGAGCT GCAGTTTGGG 
GTGGATGACA TCAACAGGAT TCGTGTGGAG AACCCTAACT CCTTGCTGGA GCAGAGCATA 
GCCTTGCTTA ACCTCTGGGT CAGCCGCGAG GGCAAGGGTG TCAAAATTGA GAACCTGTAC 
ACAGCGCTGA GGAACATTGA CCGCAGCGAG ATCATCAGCA CGTTGGAGGG CTCCGGCCGG 
CAGAGCCGCA GCATGAAGGG CAGCTGGCGT TACATGGACA GGGACTATTC ACTCTCGCCC 
TCCCAGATGA ATGGTTACGC TTCGCTGCAG GACGAGCTGC TCTCCCCTGC CTCCCTGCAT 
TATATGCTGC CATCCCCTCT GCGTGCTGAC CAGTACTGGA ACGAGGTGGC CATCATGGAC 
GCCATCCCCA TGGCCGCCAC TGAGCAGGAC GCCCTGATGG AGATGTCTGA CATGCAGGTG 
TGGTCCTCAG GGCTCACCCC TTCACTGGTG ACGGCTGAGG ACTCCTCTCT GGAGTGCAGC 
AAGGCTGAGG ACTCGGATGC TACAAGCGAA GGTCGTTTCC CAGGGCACCT TCTGACAGAT 
GCACATGTCC CAGACCACAT GGGCTCTATG GACTTAGTTG AGGATGACAC AGTGGACTCA 
GACGCCATGA ACGGGCTGAT TGAACTGCTA GAGCAGGAGG AGGGGCAGAG GCCAGAGGGG 
AAGATGCCAG CCAGCGACCA CCAGCTAGGG ACTGGAGAGC AGAACCCAGA GAGTGAAGTC 
TCTTTTGTTT CAGTTCAACA GAAGGTACAA GCCACGATCG TGCCATCGCC AACCGTGAGC 
CACACAGTGG AGAGGAGTGC AGACAGGCTG AGGGACTGGA ATGCAGAAGG CTCCTTTATC 
TCCTGCCTAC AGGACCTGAC AGAGGGCTCC TGGCAGGAGG GGGTCACCCG ACGGCTGGGT 
CCAGCACACA CCGTGGCTGC TGGGGCGCAG GGCCAGGAGC AAGAGCAGGT CCTGGCGCCG 
GACGTGGAGC TGATGCGGGT CAGCTCCACA GAGGACAGCG ACTGGCAGCC CCAGCACCCC 
GCGGGCGGCT GGCCAGAGGA GCCTGACAGC CGCTGCTTTG GGCAGGGCAA CGAAGTCCTC 
CATCTCCCTG GGGAGCATGT GACTGAGGAG CAGTTCACAG ATGAACAAGG CAATATAATC 
ACCAAGAAGG TCATCCGGAA AGTGGTGCAT CAGTTGGACC TCGACACGGA TGACAGGAAG 
GAGCATGAGG ACTTGATTCT GGAGGGCTCC CTGCAGGAGC CCCAAGACCT GGAGGCAGAG 
GCTGATCACT TCATGTACTC CATCCTACAG CGGGACGGTC TGGGGGCCAA GGAGGAGGTG 
CAAGTGCGTG TCCCAAAACT GGAAGTCTCC GGGAGCAGGA TGGGGGCTCA GATAGTGAAA 
CGAGCCAGTC TGAAAAGGGG GAAGCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK1 ( XM_015297395.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152881.1 Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X6, mRNA.

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CloneID	OGa45390
Clone ID Related Accession (Same CDS sequence)	XM_015297395.1
Accession Version	XM_015297395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ankyrin-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455999.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAC GAAAGGGCAA GTCTGGTCCA GACGTGGCTG CTGATGCTGC GACCAGCTTC 
CTGAGAGCTG CAAGATCTGG GAATCTGGAC AGAGCCTTGG ATCATCTCAG GAATGGGGTA 
GATATTAACA CCTGTAACCA GAATGGGCTG AATGCCTTGC ACCTGGCCTC CAAGGAGGGC 
CACACGAAAA TGGTGGTGGA GCTTCTGCAC AAGGAGATTG TTTTGGAGAC CACTACCAAG 
AAGGGAAACA CAGCCCTGCA CATCGCTGCC CTCGCTGGAC AGCAGGACGT GGTCCGGGAA 
CTGGTGAACT ATGGGGCCAA TGTCAATGCT CAGTCGCAGA AGGGCTTCAC ACCACTCTAC 
ATGGCAGCGC AGGAAAACCA CCTGGAAGTT GTCAAATTCC TGCTGGAAAA CGGAGCCAAC 
CAGAATGTAG CCACAGAGGA TGGCTTCACA CCACTAGCTG TGGCTCTGCA GCAGGGTCAT 
GAGAATGTGG TTGCTCACCT TATCAACTAT GGGACGAAGG GTAAGGTCCG CCTGCCTGCC 
TTGCACATTG CAGCTCGCAA CGATGACACC CGTACAGCCG CCGTGCTGCT GCAGAATGAC 
CCCAATGCTG ACGTCCTCTC CAAGACTGGA TTTACACCTT TGCACATTGC AGCCCACTAT 
GAGAATCTCA GCGTGGCCCA ATTATTGCTG AATCGTGGAG CCAGCGTCAA CTTCACACCC 
CAGAATGGGA TCACTCCTTT GCACATAGCC TCTCGTCGAG GCAACATCAT CATGGTACGG 
CTGCTGCTGG ACCGTGGGGC CCAGATAGAG ACAAGGACCA AGGATGAGTT GACGCCTCTC 
CACTGTGCAG CTCGCAATGG ACATGTGCGA ATTGCAGAGA TCCTACTGGA CCATGGTGCT 
CCCATTCAAG CCAAAACAAA GAACGGTCTG TCACCGATCC ACATGGCAGC ACAGGGTGAC 
CACCTGGACT GTGTGCGCCT ACTCCTGCAG TACAGTGCTG AGATAGACGA CATCACCCTG 
GACCACCTGA CACCACTGCA TGTAGCTGCG CACTGTGGCC ACCACCGTGT GGCCAAGCTG 
CTGGTGGAGA AGGGAGCCAA GCCCAACTCC AGAGCTCTGA ATGGCTTCAC GCCTCTCCAC 
ATTGCCTGCA AGAAGAACCA CATTCGGGTG ATGGAGTTGC TTCTGAAGAC AGGAGCCTCC 
ATTGATGCTG TCACAGAGTC TGGCCTGACC CCCTTGCATG TGGCTGCCTT CATGGGGCAC 
TTGCCCATTG TCAAAACCTT GCTGCAGCGT GGAGCATCTC CTAATGTGTC CAATGTGAAA 
GTGGAGACTC CCCTGCACAT GGCAGCCAGA GCTGGGCACA TGGATGTGGC CAAGTATCTG 
ATACAGAACA AGGCCAAAAT CAACGCCAAG GCCAAGGATG ACCAGACTCC TCTGCACTGT 
GCTGCACGCA TTGGCCACAC CAGCATGGTC CAACTCCTGC TGGAGAACAA TGCCGACCCC 
AACCTGGCCA CTACAGCAGG GCACACGCCT CTGCACATCG CTGCCAGAGA GGGGCATGTA 
GACACAGCCT TGGCCCTGCT AGAGAAGGGA GCCTCTCAGA CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TCCACGTTGC AGCCAAGTAT GGGAAGGTGG ATGTGGCAGA GCTGCTGCTG 
GTGCATGATG CTCACCCCAA TGCAGCGGGG AAGAACGGCC TGACCCCACT GCACGTAGCT 
GTGCACCACA ACAACCTGGA GATCGTCAAG CTTCTGCTTC CCAAGGGGAG CTCTCCACAC 
AGCTCAGCCT GGAACGGGTA CACCCCTCTG CACATTGCCG CCAAGCAGAA CCAGATGGAG 
GTGGCCAGCA GCTTGCTGCA GTATGGTGCT TCTGCAAATG CGGAGTCTGT TCAGGGAGTC 
ACCCCTCTGC ACCTGGCTTC TCAGGAAGGG CATACAGACA TGGTAGCACT GCTTTTCTCC 
AAGCAAGCCA ATGGAAACTT AGGCAACAAG AGTGGTCTGA CTCCTCTCCA TCTCGTGGCC 
CAAGAGGGAC ACGTACCAGT GGCTGATGTT CTGGTGAAAC ATGGAGTCAC AGTGGATGCG 
ACAACCAGGA TGGGCTACAC CTCACTGCAC ATAGCCAGCC ACTATGGGAA CATCAAGTTG 
GTGAAGTTTC TGCTGCAGCA CCAGGCTGAT GTCAATGCTA AGACTAAGCT GGGCTACACA 
CCCCTGCACC AGGCGGCACA GCAGGGCCAC ACGGATGTGG TGACCCTGCT GCTGAAGCAT 
GGTGCCTCTC CCAACGAGAT CAGCACAAAT GGCACCACTC CCCTGGCCAT TGCGAAGCGC 
CTCGGTTACA TTTCCGTCAC AGATGTGCTC AAGATAGTCA CAGAGGAGAC CACCATCCCA 
CCATTCAGTG ACAAGTACCG CATGAGCTTC CCGGAGACCG TGGATGAGAT TCTGGATGTG 
TCGGAGGATG AAGGCACTGC TCATGTCACA GTAATGGAGG AGGAGCTGGT CCCACCGAAA 
CCCAGAACAC CTGAACCCAA GGAGCAGGAG GGCAAGAGGG AGATGCTGGA GTTTGTGACC 
GTGACAACAC TGGAGCAAAT GGAGGAATCT GCAGCTGTCC CACAGGTCCC CTGCGTGCCC 
CCTGAGACTG TGGTGACCAT AGCAGAGGAG TCTGAGCAGA TGGGACCTGT GGAGACTGAG 
GCTGAGCAAG GCGGCCTGCT GCCTGCACCC TCGGTGTCCC CACAGGAGCC CTCCAAGGAG 
TTCGATGAGG ACTCCTTGAT TCCCAGCAGC CCGGCCACCG AAACCTCGGA CAACATCAGC 
CCCGTGGCCA GCCCCGTTCA CACAGGGTTC CTGGTGAGCT TCATGGTGGA TGCGCGTGGC 
GGCTCCATGC GGGGCAGCCG GCACCACGGG CTGCGCGTGG TCATCCCTCC CCGTGCCTGT 
GCTGCACCCA CCCGCATCAC TTGCCGCCTG GTGAAGCCTC AGAAGCTGCC TGCCCCCCCA 
CCGCTGGCTG AGGAGGAGGG CCTGGCAAGC CGCATCATTG CACTGGGGCC CGCTGGCGCC 
CAGTTCCTCA GCCCCGTCAT TGTGGAGATT CCTCACTTTG CCTCCTATGG ACGTGGGGAC 
CGTGAGCTGG TGGTGTTGCG CAGTGAGAAT GGCTCTGTCT GGAAGGAGCA CCGCAACCGC 
TACGAGGAGA GCTACATGGA CCAACTGCTC AGTGGCATGG ATGAGGAGCT GGAGAGCCAA 
GAGGAGCTGG AGAAGAAGAG GGTCTGCCGC ATCATCACCA CTGACTTTCC TCTCTACTTC 
GTGGTCATGT CCCGGATTTG CCAGGACTGT GACATGATTG GCCCCGAGGG AGGGTGCTTG 
AAAAGCACAC TGGTGCCCAT GGTGCAGGCC ACCTTCCCAG ACACCGCTGT CACCAAGAAA 
GTGAGGCTGG CTCTGCAGGC GCAGCCCGTG CCTGATGAGC TGGTGACCAA GCTGCTGGGG 
AACCAGGCAA CCTTCAGCCC CATCGTCACA GTGGAGCCAC GTCGGAGGAA GTTCCACCGC 
CCCATTGGCC TCCGCATCCC TCTGCCCCCA TCCTGGAAGG ACAATCCCCG GGACAGCGGC 
GAGGGTGACA CCACCAGCCT GCGCCTGCTC TGTTCTGTGA TAGGAGGGAC AGCACAAGCT 
CAGTGGGAAG ACATCACAGG CACCACAAAG CTGGTCTATG AAAAAGAGTG TGCTAACTTT 
ACCACCAATG TGTCTGCCAG GTTCTGGCTG GCTGACTGCC CGCGCACTGC TGAGGCTGTG 
CACTTTGCCA CCGTGCTGTA CAAGGAACTG ACGGCTGTGC CCTACATGGC CAAATTTGTG 
GTGTTTGCTA AGATGAACGA TGGACGGGAA GGCCGGCTGC GCTGCTACTG CATGACTGAT 
GACAAGGTGG ACAAGACTTT GGAGCATCAT GAGAACTTCA CTGAGGTGGC CCGCAGCAGG 
GACATTGAGG TGGTGGAAGG AATGCCTCTG CATGTCGAGC TCTCAGGGAA CCTGGTGCCC 
GTCAAGAAGG CCACGCAGTC ACGCACCTTC TTCTTCCAGT CCTTCCGGGA AAGCCGCCTC 
GCCATCCCCA TCAAGGTCCG GGACAGCAGC CGGGAAGCCA GTGGCTCCCT GTCCTTCCTG 
CGCAAGGCCA TGAAGTATGA GGACTTGCAG CATGTGCTCT GCCACCTCAA CATCAGCATT 
CCACCCTGCA CCAAGGGAAG CAGCACTGAG GAGCGAAGGA GGACACTGAC ACCTTTGTCT 
CTGCGCTCTC TGAGCAGCAC CGACAGGGCA GACCAGAAGA TGATCGACAT TGCAGAGCAG 
CTTGGCCTCA GCTGGGCAGA GCTGGCACGT GAGCTGCAGT TTGGGGTGGA TGACATCAAC 
AGGATTCGTG TGGAGAACCC TAACTCCTTG CTGGAGCAGA GCATAGCCTT GCTTAACCTC 
TGGGTCAGCC GCGAGGGCAA GGGTGTCAAA ATTGAGAACC TGTACACAGC GCTGAGGAAC 
ATTGACCGCA GCGAGATCAT CAGCACGTTG GAGGGCTCCG GCCGGCAGAG CCGCAGCATG 
AAGGGCAGCT GGCGTTACAT GGACAGGGAC TATTCACTCT CGCCCTCCCA GATGAATGGT 
TACGCTTCGC TGCAGGACGA GCTGCTCTCC CCTGCCTCCC TGCATTATAT GCTGCCATCC 
CCTCTGCGTG CTGACCAGTA CTGGAACGAG GTGGCCATCA TGGACGCCAT CCCCATGGCC 
GCCACTGAGC AGGACGCCCT GATGGAGATG TCTGACATGC AGGTGTGGTC CTCAGGGCTC 
ACCCCTTCAC TGGTGACGGC TGAGGACTCC TCTCTGGAGT GCAGCAAGGC TGAGGACTCG 
GATGCTACAA GCGAAGGTCG TTTCCCAGGG CACCTTCTGA CAGATGCACA TGTCCCAGAC 
CACATGGGCT CTATGGACTT AGTTGAGGAT GACACAGTGG ACTCAGACGC CATGAACGGG 
CTGATTGAAC TGCTAGAGCA GGAGGAGGGG CAGAGGCCAG AGGGGAAGAT GCCAGCCAGC 
GACCACCAGC TAGGGACTGG AGAGCAGAAC CCAGAGAGTG AAGTCTCTTT TGTTTCAGTT 
CAACAGAAGG TACAAGCCAC GATCGTGCCA TCGCCAACCG TGAGCCACAC AGTGGAGAGG 
AGTGCAGACA GGCTGAGGGA CTGGAATGCA GAAGGCTCCT TTATCTCCTG CCTACAGGAC 
CTGACAGAGG GCTCCTGGCA GGAGGGGGTC ACCCGACGGC TGGGTCCAGC ACACACCGTG 
GCTGCTGGGG CGCAGGGCCA GGAGCAAGAG CAGGTCCTGG CGCCGGACGT GGAGCTGATG 
CGGGTCAGCT CCACAGAGGA CAGCGACTGG CAGCCCCAGC ACCCCGCGGG CGGCTGGCCA 
GAGGAGCCTG ACAGCCGCTG CTTTGGGCAG GGCAACGAAG TCCTCCATCT CCCTGGGGAG 
CATGTGACTG AGGAGCAGTT CACAGATGAA CAAGGCAATA TAATCACCAA GAAGGTCATC 
CGGAAAGTGG TGCATCAGTT GGACCTCGAC ACGGATGACA GGAAGGAGCA TGAGGACTTG 
ATTCTGGAGG GCTCCCTGCA GGAGCCCCAA GACCTGGAGG CAGAGGCTGA TCACTTCATG 
TACTCCATCC TACAGCGGGA CGGTCTGGGG GCCAAGGAGG AGGTGCAAGT GCGTGTCCCA 
AAACTGGAAG TCTCCGGGAG CAGGATGGGG GCTCAGATAG TGAAACGAGC CAGTCTGAAA 
AGGGGGAAGC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152881.1
CDS	16..5790
Translation	MAARKGKSGPDVAADAATSFLRAARSGNLDRALDHLRNGVDINTCNQNGLNALHLASKEGHTKMVVELLHKEIVLETTTKKGNTALHIAALAGQQDVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLSVAQLLLNRGASVNFTPQNGITPLHIASRRGNIIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRIAEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYSAEIDDITLDHLTPLHVAAHCGHHRVAKLLVEKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVAAFMGHLPIVKTLLQRGASPNVSNVKVETPLHMAARAGHMDVAKYLIQNKAKINAKAKDDQTPLHCAARIGHTSMVQLLLENNADPNLATTAGHTPLHIAAREGHVDTALALLEKGASQTCMTKKGFTPLHVAAKYGKVDVAELLLVHDAHPNAAGKNGLTPLHVAVHHNNLEIVKLLLPKGSSPHSSAWNGYTPLHIAAKQNQMEVASSLLQYGASANAESVQGVTPLHLASQEGHTDMVALLFSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLVKHGVTVDATTRMGYTSLHIASHYGNIKLVKFLLQHQADVNAKTKLGYTPLHQAAQQGHTDVVTLLLKHGASPNEISTNGTTPLAIAKRLGYISVTDVLKIVTEETTIPPFSDKYRMSFPETVDEILDVSEDEGTAHVTVMEEELVPPKPRTPEPKEQEGKREMLEFVTVTTLEQMEESAAVPQVPCVPPETVVTIAEESEQMGPVETEAEQGGLLPAPSVSPQEPSKEFDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHHGLRVVIPPRACAAPTRITCRLVKPQKLPAPPPLAEEEGLASRIIALGPAGAQFLSPVIVEIPHFASYGRGDRELVVLRSENGSVWKEHRNRYEESYMDQLLSGMDEELESQEELEKKRVCRIITTDFPLYFVVMSRICQDCDMIGPEGGCLKSTLVPMVQATFPDTAVTKKVRLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWKDNPRDSGEGDTTSLRLLCSVIGGTAQAQWEDITGTTKLVYEKECANFTTNVSARFWLADCPRTAEAVHFATVLYKELTAVPYMAKFVVFAKMNDGREGRLRCYCMTDDKVDKTLEHHENFTEVARSRDIEVVEGMPLHVELSGNLVPVKKATQSRTFFFQSFRESRLAIPIKVRDSSREASGSLSFLRKAMKYEDLQHVLCHLNISIPPCTKGSSTEERRRTLTPLSLRSLSSTDRADQKMIDIAEQLGLSWAELARELQFGVDDINRIRVENPNSLLEQSIALLNLWVSREGKGVKIENLYTALRNIDRSEIISTLEGSGRQSRSMKGSWRYMDRDYSLSPSQMNGYASLQDELLSPASLHYMLPSPLRADQYWNEVAIMDAIPMAATEQDALMEMSDMQVWSSGLTPSLVTAEDSSLECSKAEDSDATSEGRFPGHLLTDAHVPDHMGSMDLVEDDTVDSDAMNGLIELLEQEEGQRPEGKMPASDHQLGTGEQNPESEVSFVSVQQKVQATIVPSPTVSHTVERSADRLRDWNAEGSFISCLQDLTEGSWQEGVTRRLGPAHTVAAGAQGQEQEQVLAPDVELMRVSSTEDSDWQPQHPAGGWPEEPDSRCFGQGNEVLHLPGEHVTEEQFTDEQGNIITKKVIRKVVHQLDLDTDDRKEHEDLILEGSLQEPQDLEAEADHFMYSILQRDGLGAKEEVQVRVPKLEVSGSRMGAQIVKRASLKRGKH

Target ORF information:

RefSeq Version	XM_015297395.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297395.1

ORF Insert Sequence:

ATGGCAGCAC GAAAGGGCAA GTCTGGTCCA GACGTGGCTG CTGATGCTGC GACCAGCTTC 
CTGAGAGCTG CAAGATCTGG GAATCTGGAC AGAGCCTTGG ATCATCTCAG GAATGGGGTA 
GATATTAACA CCTGTAACCA GAATGGGCTG AATGCCTTGC ACCTGGCCTC CAAGGAGGGC 
CACACGAAAA TGGTGGTGGA GCTTCTGCAC AAGGAGATTG TTTTGGAGAC CACTACCAAG 
AAGGGAAACA CAGCCCTGCA CATCGCTGCC CTCGCTGGAC AGCAGGACGT GGTCCGGGAA 
CTGGTGAACT ATGGGGCCAA TGTCAATGCT CAGTCGCAGA AGGGCTTCAC ACCACTCTAC 
ATGGCAGCGC AGGAAAACCA CCTGGAAGTT GTCAAATTCC TGCTGGAAAA CGGAGCCAAC 
CAGAATGTAG CCACAGAGGA TGGCTTCACA CCACTAGCTG TGGCTCTGCA GCAGGGTCAT 
GAGAATGTGG TTGCTCACCT TATCAACTAT GGGACGAAGG GTAAGGTCCG CCTGCCTGCC 
TTGCACATTG CAGCTCGCAA CGATGACACC CGTACAGCCG CCGTGCTGCT GCAGAATGAC 
CCCAATGCTG ACGTCCTCTC CAAGACTGGA TTTACACCTT TGCACATTGC AGCCCACTAT 
GAGAATCTCA GCGTGGCCCA ATTATTGCTG AATCGTGGAG CCAGCGTCAA CTTCACACCC 
CAGAATGGGA TCACTCCTTT GCACATAGCC TCTCGTCGAG GCAACATCAT CATGGTACGG 
CTGCTGCTGG ACCGTGGGGC CCAGATAGAG ACAAGGACCA AGGATGAGTT GACGCCTCTC 
CACTGTGCAG CTCGCAATGG ACATGTGCGA ATTGCAGAGA TCCTACTGGA CCATGGTGCT 
CCCATTCAAG CCAAAACAAA GAACGGTCTG TCACCGATCC ACATGGCAGC ACAGGGTGAC 
CACCTGGACT GTGTGCGCCT ACTCCTGCAG TACAGTGCTG AGATAGACGA CATCACCCTG 
GACCACCTGA CACCACTGCA TGTAGCTGCG CACTGTGGCC ACCACCGTGT GGCCAAGCTG 
CTGGTGGAGA AGGGAGCCAA GCCCAACTCC AGAGCTCTGA ATGGCTTCAC GCCTCTCCAC 
ATTGCCTGCA AGAAGAACCA CATTCGGGTG ATGGAGTTGC TTCTGAAGAC AGGAGCCTCC 
ATTGATGCTG TCACAGAGTC TGGCCTGACC CCCTTGCATG TGGCTGCCTT CATGGGGCAC 
TTGCCCATTG TCAAAACCTT GCTGCAGCGT GGAGCATCTC CTAATGTGTC CAATGTGAAA 
GTGGAGACTC CCCTGCACAT GGCAGCCAGA GCTGGGCACA TGGATGTGGC CAAGTATCTG 
ATACAGAACA AGGCCAAAAT CAACGCCAAG GCCAAGGATG ACCAGACTCC TCTGCACTGT 
GCTGCACGCA TTGGCCACAC CAGCATGGTC CAACTCCTGC TGGAGAACAA TGCCGACCCC 
AACCTGGCCA CTACAGCAGG GCACACGCCT CTGCACATCG CTGCCAGAGA GGGGCATGTA 
GACACAGCCT TGGCCCTGCT AGAGAAGGGA GCCTCTCAGA CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TCCACGTTGC AGCCAAGTAT GGGAAGGTGG ATGTGGCAGA GCTGCTGCTG 
GTGCATGATG CTCACCCCAA TGCAGCGGGG AAGAACGGCC TGACCCCACT GCACGTAGCT 
GTGCACCACA ACAACCTGGA GATCGTCAAG CTTCTGCTTC CCAAGGGGAG CTCTCCACAC 
AGCTCAGCCT GGAACGGGTA CACCCCTCTG CACATTGCCG CCAAGCAGAA CCAGATGGAG 
GTGGCCAGCA GCTTGCTGCA GTATGGTGCT TCTGCAAATG CGGAGTCTGT TCAGGGAGTC 
ACCCCTCTGC ACCTGGCTTC TCAGGAAGGG CATACAGACA TGGTAGCACT GCTTTTCTCC 
AAGCAAGCCA ATGGAAACTT AGGCAACAAG AGTGGTCTGA CTCCTCTCCA TCTCGTGGCC 
CAAGAGGGAC ACGTACCAGT GGCTGATGTT CTGGTGAAAC ATGGAGTCAC AGTGGATGCG 
ACAACCAGGA TGGGCTACAC CTCACTGCAC ATAGCCAGCC ACTATGGGAA CATCAAGTTG 
GTGAAGTTTC TGCTGCAGCA CCAGGCTGAT GTCAATGCTA AGACTAAGCT GGGCTACACA 
CCCCTGCACC AGGCGGCACA GCAGGGCCAC ACGGATGTGG TGACCCTGCT GCTGAAGCAT 
GGTGCCTCTC CCAACGAGAT CAGCACAAAT GGCACCACTC CCCTGGCCAT TGCGAAGCGC 
CTCGGTTACA TTTCCGTCAC AGATGTGCTC AAGATAGTCA CAGAGGAGAC CACCATCCCA 
CCATTCAGTG ACAAGTACCG CATGAGCTTC CCGGAGACCG TGGATGAGAT TCTGGATGTG 
TCGGAGGATG AAGGCACTGC TCATGTCACA GTAATGGAGG AGGAGCTGGT CCCACCGAAA 
CCCAGAACAC CTGAACCCAA GGAGCAGGAG GGCAAGAGGG AGATGCTGGA GTTTGTGACC 
GTGACAACAC TGGAGCAAAT GGAGGAATCT GCAGCTGTCC CACAGGTCCC CTGCGTGCCC 
CCTGAGACTG TGGTGACCAT AGCAGAGGAG TCTGAGCAGA TGGGACCTGT GGAGACTGAG 
GCTGAGCAAG GCGGCCTGCT GCCTGCACCC TCGGTGTCCC CACAGGAGCC CTCCAAGGAG 
TTCGATGAGG ACTCCTTGAT TCCCAGCAGC CCGGCCACCG AAACCTCGGA CAACATCAGC 
CCCGTGGCCA GCCCCGTTCA CACAGGGTTC CTGGTGAGCT TCATGGTGGA TGCGCGTGGC 
GGCTCCATGC GGGGCAGCCG GCACCACGGG CTGCGCGTGG TCATCCCTCC CCGTGCCTGT 
GCTGCACCCA CCCGCATCAC TTGCCGCCTG GTGAAGCCTC AGAAGCTGCC TGCCCCCCCA 
CCGCTGGCTG AGGAGGAGGG CCTGGCAAGC CGCATCATTG CACTGGGGCC CGCTGGCGCC 
CAGTTCCTCA GCCCCGTCAT TGTGGAGATT CCTCACTTTG CCTCCTATGG ACGTGGGGAC 
CGTGAGCTGG TGGTGTTGCG CAGTGAGAAT GGCTCTGTCT GGAAGGAGCA CCGCAACCGC 
TACGAGGAGA GCTACATGGA CCAACTGCTC AGTGGCATGG ATGAGGAGCT GGAGAGCCAA 
GAGGAGCTGG AGAAGAAGAG GGTCTGCCGC ATCATCACCA CTGACTTTCC TCTCTACTTC 
GTGGTCATGT CCCGGATTTG CCAGGACTGT GACATGATTG GCCCCGAGGG AGGGTGCTTG 
AAAAGCACAC TGGTGCCCAT GGTGCAGGCC ACCTTCCCAG ACACCGCTGT CACCAAGAAA 
GTGAGGCTGG CTCTGCAGGC GCAGCCCGTG CCTGATGAGC TGGTGACCAA GCTGCTGGGG 
AACCAGGCAA CCTTCAGCCC CATCGTCACA GTGGAGCCAC GTCGGAGGAA GTTCCACCGC 
CCCATTGGCC TCCGCATCCC TCTGCCCCCA TCCTGGAAGG ACAATCCCCG GGACAGCGGC 
GAGGGTGACA CCACCAGCCT GCGCCTGCTC TGTTCTGTGA TAGGAGGGAC AGCACAAGCT 
CAGTGGGAAG ACATCACAGG CACCACAAAG CTGGTCTATG AAAAAGAGTG TGCTAACTTT 
ACCACCAATG TGTCTGCCAG GTTCTGGCTG GCTGACTGCC CGCGCACTGC TGAGGCTGTG 
CACTTTGCCA CCGTGCTGTA CAAGGAACTG ACGGCTGTGC CCTACATGGC CAAATTTGTG 
GTGTTTGCTA AGATGAACGA TGGACGGGAA GGCCGGCTGC GCTGCTACTG CATGACTGAT 
GACAAGGTGG ACAAGACTTT GGAGCATCAT GAGAACTTCA CTGAGGTGGC CCGCAGCAGG 
GACATTGAGG TGGTGGAAGG AATGCCTCTG CATGTCGAGC TCTCAGGGAA CCTGGTGCCC 
GTCAAGAAGG CCACGCAGTC ACGCACCTTC TTCTTCCAGT CCTTCCGGGA AAGCCGCCTC 
GCCATCCCCA TCAAGGTCCG GGACAGCAGC CGGGAAGCCA GTGGCTCCCT GTCCTTCCTG 
CGCAAGGCCA TGAAGTATGA GGACTTGCAG CATGTGCTCT GCCACCTCAA CATCAGCATT 
CCACCCTGCA CCAAGGGAAG CAGCACTGAG GAGCGAAGGA GGACACTGAC ACCTTTGTCT 
CTGCGCTCTC TGAGCAGCAC CGACAGGGCA GACCAGAAGA TGATCGACAT TGCAGAGCAG 
CTTGGCCTCA GCTGGGCAGA GCTGGCACGT GAGCTGCAGT TTGGGGTGGA TGACATCAAC 
AGGATTCGTG TGGAGAACCC TAACTCCTTG CTGGAGCAGA GCATAGCCTT GCTTAACCTC 
TGGGTCAGCC GCGAGGGCAA GGGTGTCAAA ATTGAGAACC TGTACACAGC GCTGAGGAAC 
ATTGACCGCA GCGAGATCAT CAGCACGTTG GAGGGCTCCG GCCGGCAGAG CCGCAGCATG 
AAGGGCAGCT GGCGTTACAT GGACAGGGAC TATTCACTCT CGCCCTCCCA GATGAATGGT 
TACGCTTCGC TGCAGGACGA GCTGCTCTCC CCTGCCTCCC TGCATTATAT GCTGCCATCC 
CCTCTGCGTG CTGACCAGTA CTGGAACGAG GTGGCCATCA TGGACGCCAT CCCCATGGCC 
GCCACTGAGC AGGACGCCCT GATGGAGATG TCTGACATGC AGGTGTGGTC CTCAGGGCTC 
ACCCCTTCAC TGGTGACGGC TGAGGACTCC TCTCTGGAGT GCAGCAAGGC TGAGGACTCG 
GATGCTACAA GCGAAGGTCG TTTCCCAGGG CACCTTCTGA CAGATGCACA TGTCCCAGAC 
CACATGGGCT CTATGGACTT AGTTGAGGAT GACACAGTGG ACTCAGACGC CATGAACGGG 
CTGATTGAAC TGCTAGAGCA GGAGGAGGGG CAGAGGCCAG AGGGGAAGAT GCCAGCCAGC 
GACCACCAGC TAGGGACTGG AGAGCAGAAC CCAGAGAGTG AAGTCTCTTT TGTTTCAGTT 
CAACAGAAGG TACAAGCCAC GATCGTGCCA TCGCCAACCG TGAGCCACAC AGTGGAGAGG 
AGTGCAGACA GGCTGAGGGA CTGGAATGCA GAAGGCTCCT TTATCTCCTG CCTACAGGAC 
CTGACAGAGG GCTCCTGGCA GGAGGGGGTC ACCCGACGGC TGGGTCCAGC ACACACCGTG 
GCTGCTGGGG CGCAGGGCCA GGAGCAAGAG CAGGTCCTGG CGCCGGACGT GGAGCTGATG 
CGGGTCAGCT CCACAGAGGA CAGCGACTGG CAGCCCCAGC ACCCCGCGGG CGGCTGGCCA 
GAGGAGCCTG ACAGCCGCTG CTTTGGGCAG GGCAACGAAG TCCTCCATCT CCCTGGGGAG 
CATGTGACTG AGGAGCAGTT CACAGATGAA CAAGGCAATA TAATCACCAA GAAGGTCATC 
CGGAAAGTGG TGCATCAGTT GGACCTCGAC ACGGATGACA GGAAGGAGCA TGAGGACTTG 
ATTCTGGAGG GCTCCCTGCA GGAGCCCCAA GACCTGGAGG CAGAGGCTGA TCACTTCATG 
TACTCCATCC TACAGCGGGA CGGTCTGGGG GCCAAGGAGG AGGTGCAAGT GCGTGTCCCA 
AAACTGGAAG TCTCCGGGAG CAGGATGGGG GCTCAGATAG TGAAACGAGC CAGTCTGAAA 
AGGGGGAAGC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK1 ( XM_015297396.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152882.1 Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X7, mRNA.

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CloneID	OGa45391
Clone ID Related Accession (Same CDS sequence)	XM_015297396.1
Accession Version	XM_015297396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ankyrin-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455999.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATGCT TGCATTACTT CAGGGCTGAT GCTGCGACCA GCTTCCTGAG AGCTGCAAGA 
TCTGGGAATC TGGACAGAGC CTTGGATCAT CTCAGGAATG GGGTAGATAT TAACACCTGT 
AACCAGAATG GGCTGAATGC CTTGCACCTG GCCTCCAAGG AGGGCCACAC GAAAATGGTG 
GTGGAGCTTC TGCACAAGGA GATTGTTTTG GAGACCACTA CCAAGAAGGG AAACACAGCC 
CTGCACATCG CTGCCCTCGC TGGACAGCAG GACGTGGTCC GGGAACTGGT GAACTATGGG 
GCCAATGTCA ATGCTCAGTC GCAGAAGGGC TTCACACCAC TCTACATGGC AGCGCAGGAA 
AACCACCTGG AAGTTGTCAA ATTCCTGCTG GAAAACGGAG CCAACCAGAA TGTAGCCACA 
GAGGATGGCT TCACACCACT AGCTGTGGCT CTGCAGCAGG GTCATGAGAA TGTGGTTGCT 
CACCTTATCA ACTATGGGAC GAAGGGTAAG GTCCGCCTGC CTGCCTTGCA CATTGCAGCT 
CGCAACGATG ACACCCGTAC AGCCGCCGTG CTGCTGCAGA ATGACCCCAA TGCTGACGTC 
CTCTCCAAGA CTGGATTTAC ACCTTTGCAC ATTGCAGCCC ACTATGAGAA TCTCAGCGTG 
GCCCAATTAT TGCTGAATCG TGGAGCCAGC GTCAACTTCA CACCCCAGAA TGGGATCACT 
CCTTTGCACA TAGCCTCTCG TCGAGGCAAC ATCATCATGG TACGGCTGCT GCTGGACCGT 
GGGGCCCAGA TAGAGACAAG GACCAAGGAT GAGTTGACGC CTCTCCACTG TGCAGCTCGC 
AATGGACATG TGCGAATTGC AGAGATCCTA CTGGACCATG GTGCTCCCAT TCAAGCCAAA 
ACAAAGAACG GTCTGTCACC GATCCACATG GCAGCACAGG GTGACCACCT GGACTGTGTG 
CGCCTACTCC TGCAGTACAG TGCTGAGATA GACGACATCA CCCTGGACCA CCTGACACCA 
CTGCATGTAG CTGCGCACTG TGGCCACCAC CGTGTGGCCA AGCTGCTGGT GGAGAAGGGA 
GCCAAGCCCA ACTCCAGAGC TCTGAATGGC TTCACGCCTC TCCACATTGC CTGCAAGAAG 
AACCACATTC GGGTGATGGA GTTGCTTCTG AAGACAGGAG CCTCCATTGA TGCTGTCACA 
GAGTCTGGCC TGACCCCCTT GCATGTGGCT GCCTTCATGG GGCACTTGCC CATTGTCAAA 
ACCTTGCTGC AGCGTGGAGC ATCTCCTAAT GTGTCCAATG TGAAAGTGGA GACTCCCCTG 
CACATGGCAG CCAGAGCTGG GCACATGGAT GTGGCCAAGT ATCTGATACA GAACAAGGCC 
AAAATCAACG CCAAGGCCAA GGATGACCAG ACTCCTCTGC ACTGTGCTGC ACGCATTGGC 
CACACCAGCA TGGTCCAACT CCTGCTGGAG AACAATGCCG ACCCCAACCT GGCCACTACA 
GCAGGGCACA CGCCTCTGCA CATCGCTGCC AGAGAGGGGC ATGTAGACAC AGCCTTGGCC 
CTGCTAGAGA AGGGAGCCTC TCAGACCTGC ATGACCAAGA AAGGATTTAC CCCTCTCCAC 
GTTGCAGCCA AGTATGGGAA GGTGGATGTG GCAGAGCTGC TGCTGGTGCA TGATGCTCAC 
CCCAATGCAG CGGGGAAGAA CGGCCTGACC CCACTGCACG TAGCTGTGCA CCACAACAAC 
CTGGAGATCG TCAAGCTTCT GCTTCCCAAG GGGAGCTCTC CACACAGCTC AGCCTGGAAC 
GGGTACACCC CTCTGCACAT TGCCGCCAAG CAGAACCAGA TGGAGGTGGC CAGCAGCTTG 
CTGCAGTATG GTGCTTCTGC AAATGCGGAG TCTGTTCAGG GAGTCACCCC TCTGCACCTG 
GCTTCTCAGG AAGGGCATAC AGACATGGTA GCACTGCTTT TCTCCAAGCA AGCCAATGGA 
AACTTAGGCA ACAAGAGTGG TCTGACTCCT CTCCATCTCG TGGCCCAAGA GGGACACGTA 
CCAGTGGCTG ATGTTCTGGT GAAACATGGA GTCACAGTGG ATGCGACAAC CAGGATGGGC 
TACACCTCAC TGCACATAGC CAGCCACTAT GGGAACATCA AGTTGGTGAA GTTTCTGCTG 
CAGCACCAGG CTGATGTCAA TGCTAAGACT AAGCTGGGCT ACACACCCCT GCACCAGGCG 
GCACAGCAGG GCCACACGGA TGTGGTGACC CTGCTGCTGA AGCATGGTGC CTCTCCCAAC 
GAGATCAGCA CAAATGGCAC CACTCCCCTG GCCATTGCGA AGCGCCTCGG TTACATTTCC 
GTCACAGATG TGCTCAAGAT AGTCACAGAG GAGACCACCA TCCCACCATT CAGTGACAAG 
TACCGCATGA GCTTCCCGGA GACCGTGGAT GAGATTCTGG ATGTGTCGGA GGATGAAGGC 
ACTGCTCATG TCACAGTAAT GGAGGAGGAG CTGGTCCCAC CGAAACCCAG AACACCTGAA 
CCCAAGGAGC AGGAGGGCAA GAGGGAGATG CTGGAGTTTG TGACCGTGAC AACACTGGAG 
CAAATGGAGG AATCTGCAGC TGTCCCACAG GTCCCCTGCG TGCCCCCTGA GACTGTGGTG 
ACCATAGCAG AGGAGTCTGA GCAGATGGGA CCTGTGGAGA CTGAGGCTGA GCAAGGCGGC 
CTGCTGCCTG CACCCTCGGT GTCCCCACAG GAGCCCTCCA AGGAGTTCGA TGAGGACTCC 
TTGATTCCCA GCAGCCCGGC CACCGAAACC TCGGACAACA TCAGCCCCGT GGCCAGCCCC 
GTTCACACAG GGTTCCTGGT GAGCTTCATG GTGGATGCGC GTGGCGGCTC CATGCGGGGC 
AGCCGGCACC ACGGGCTGCG CGTGGTCATC CCTCCCCGTG CCTGTGCTGC ACCCACCCGC 
ATCACTTGCC GCCTGGTGAA GCCTCAGAAG CTGCCTGCCC CCCCACCGCT GGCTGAGGAG 
GAGGGCCTGG CAAGCCGCAT CATTGCACTG GGGCCCGCTG GCGCCCAGTT CCTCAGCCCC 
GTCATTGTGG AGATTCCTCA CTTTGCCTCC TATGGACGTG GGGACCGTGA GCTGGTGGTG 
TTGCGCAGTG AGAATGGCTC TGTCTGGAAG GAGCACCGCA ACCGCTACGA GGAGAGCTAC 
ATGGACCAAC TGCTCAGTGG CATGGATGAG GAGCTGGAGA GCCAAGAGGA GCTGGAGAAG 
AAGAGGGTCT GCCGCATCAT CACCACTGAC TTTCCTCTCT ACTTCGTGGT CATGTCCCGG 
ATTTGCCAGG ACTGTGACAT GATTGGCCCC GAGGGAGGGT GCTTGAAAAG CACACTGGTG 
CCCATGGTGC AGGCCACCTT CCCAGACACC GCTGTCACCA AGAAAGTGAG GCTGGCTCTG 
CAGGCGCAGC CCGTGCCTGA TGAGCTGGTG ACCAAGCTGC TGGGGAACCA GGCAACCTTC 
AGCCCCATCG TCACAGTGGA GCCACGTCGG AGGAAGTTCC ACCGCCCCAT TGGCCTCCGC 
ATCCCTCTGC CCCCATCCTG GAAGGACAAT CCCCGGGACA GCGGCGAGGG TGACACCACC 
AGCCTGCGCC TGCTCTGTTC TGTGATAGGA GGGACAGCAC AAGCTCAGTG GGAAGACATC 
ACAGGCACCA CAAAGCTGGT CTATGAAAAA GAGTGTGCTA ACTTTACCAC CAATGTGTCT 
GCCAGGTTCT GGCTGGCTGA CTGCCCGCGC ACTGCTGAGG CTGTGCACTT TGCCACCGTG 
CTGTACAAGG AACTGACGGC TGTGCCCTAC ATGGCCAAAT TTGTGGTGTT TGCTAAGATG 
AACGATGGAC GGGAAGGCCG GCTGCGCTGC TACTGCATGA CTGATGACAA GGTGGACAAG 
ACTTTGGAGC ATCATGAGAA CTTCACTGAG GTGGCCCGCA GCAGGGACAT TGAGGTGGTG 
GAAGGAATGC CTCTGCATGT CGAGCTCTCA GGGAACCTGG TGCCCGTCAA GAAGGCCACG 
CAGTCACGCA CCTTCTTCTT CCAGTCCTTC CGGGAAAGCC GCCTCGCCAT CCCCATCAAG 
GTCCGGGACA GCAGCCGGGA AGCCAGTGGC TCCCTGTCCT TCCTGCGCAA GGCCATGAAG 
TATGAGGACT TGCAGCATGT GCTCTGCCAC CTCAACATCA GCATTCCACC CTGCACCAAG 
GGAAGCAGCA CTGAGGAGCG AAGGAGGACA CTGACACCTT TGTCTCTGCG CTCTCTGAGC 
AGCACCGACA GGGCAGACCA GAAGATGATC GACATTGCAG AGCAGCTTGG CCTCAGCTGG 
GCAGAGCTGG CACGTGAGCT GCAGTTTGGG GTGGATGACA TCAACAGGAT TCGTGTGGAG 
AACCCTAACT CCTTGCTGGA GCAGAGCATA GCCTTGCTTA ACCTCTGGGT CAGCCGCGAG 
GGCAAGGGTG TCAAAATTGA GAACCTGTAC ACAGCGCTGA GGAACATTGA CCGCAGCGAG 
ATCATCAGCA CGTTGGAGGG CTCCGGCCGG CAGAGCCGCA GCATGAAGGG CAGCTGGCGT 
TACATGGACA GGGACTATTC ACTCTCGCCC TCCCAGATGA ATGGTTACGC TTCGCTGCAG 
GACGAGCTGC TCTCCCCTGC CTCCCTGCAT TATATGCTGC CATCCCCTCT GCGTGCTGAC 
CAGTACTGGA ACGAGGTGGC CATCATGGAC GCCATCCCCA TGGCCGCCAC TGAGCAGGAC 
GCCCTGATGG AGATGTCTGA CATGCAGGTG TGGTCCTCAG GGCTCACCCC TTCACTGGTG 
ACGGCTGAGG ACTCCTCTCT GGAGTGCAGC AAGGCTGAGG ACTCGGATGC TACAAGCGAA 
GGTCGTTTCC CAGGGCACCT TCTGACAGAT GCACATGTCC CAGACCACAT GGGCTCTATG 
GACTTAGTTG AGGATGACAC AGTGGACTCA GACGCCATGA ACGGGCTGAT TGAACTGCTA 
GAGCAGGAGG AGGGGCAGAG GCCAGAGGGG AAGATGCCAG CCAGCGACCA CCAGCTAGGG 
ACTGGAGAGC AGAACCCAGA GAGTGAAGTC TCTTTTGTTT CAGTTCAACA GAAGGTACAA 
GCCACGATCG TGCCATCGCC AACCGTGAGC CACACAGTGG AGAGGAGTGC AGACAGGCTG 
AGGGACTGGA ATGCAGAAGG CTCCTTTATC TCCTGCCTAC AGGACCTGAC AGAGGGCTCC 
TGGCAGGAGG GGGTCACCCG ACGGCTGGGT CCAGCACACA CCGTGGCTGC TGGGGCGCAG 
GGCCAGGAGC AAGAGCAGGT CCTGGCGCCG GACGTGGAGC TGATGCGGGT CAGCTCCACA 
GAGGACAGCG ACTGGCAGCC CCAGCACCCC GCGGGCGGCT GGCCAGAGGA GCCTGACAGC 
CGCTGCTTTG GGCAGGGCAA CGAAGTCCTC CATCTCCCTG GGGAGCATGT GACTGAGGAG 
CAGTTCACAG ATGAACAAGG CAATATAATC ACCAAGAAGG TCATCCGGAA AGTGGTGCAT 
CAGTTGGACC TCGACACGGA TGACAGGAAG GAGCATGAGG ACTTGATTCT GGAGGGCTCC 
CTGCAGGAGC CCCAAGACCT GGAGGCAGAG GCTGATCACT TCATGTACTC CATCCTACAG 
CGGGACGGTC TGGGGGCCAA GGAGGAGGTG CAAGTGCGTG TCCCAAAACT GGAAGTCTCC 
GGGAGCAGGA TGGGGGCTCA GATAGTGAAA CGAGCCAGTC TGAAAAGGGG GAAGCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152882.1
CDS	80..5839
Translation	MPCLHYFRADAATSFLRAARSGNLDRALDHLRNGVDINTCNQNGLNALHLASKEGHTKMVVELLHKEIVLETTTKKGNTALHIAALAGQQDVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLSVAQLLLNRGASVNFTPQNGITPLHIASRRGNIIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRIAEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYSAEIDDITLDHLTPLHVAAHCGHHRVAKLLVEKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVAAFMGHLPIVKTLLQRGASPNVSNVKVETPLHMAARAGHMDVAKYLIQNKAKINAKAKDDQTPLHCAARIGHTSMVQLLLENNADPNLATTAGHTPLHIAAREGHVDTALALLEKGASQTCMTKKGFTPLHVAAKYGKVDVAELLLVHDAHPNAAGKNGLTPLHVAVHHNNLEIVKLLLPKGSSPHSSAWNGYTPLHIAAKQNQMEVASSLLQYGASANAESVQGVTPLHLASQEGHTDMVALLFSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLVKHGVTVDATTRMGYTSLHIASHYGNIKLVKFLLQHQADVNAKTKLGYTPLHQAAQQGHTDVVTLLLKHGASPNEISTNGTTPLAIAKRLGYISVTDVLKIVTEETTIPPFSDKYRMSFPETVDEILDVSEDEGTAHVTVMEEELVPPKPRTPEPKEQEGKREMLEFVTVTTLEQMEESAAVPQVPCVPPETVVTIAEESEQMGPVETEAEQGGLLPAPSVSPQEPSKEFDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHHGLRVVIPPRACAAPTRITCRLVKPQKLPAPPPLAEEEGLASRIIALGPAGAQFLSPVIVEIPHFASYGRGDRELVVLRSENGSVWKEHRNRYEESYMDQLLSGMDEELESQEELEKKRVCRIITTDFPLYFVVMSRICQDCDMIGPEGGCLKSTLVPMVQATFPDTAVTKKVRLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWKDNPRDSGEGDTTSLRLLCSVIGGTAQAQWEDITGTTKLVYEKECANFTTNVSARFWLADCPRTAEAVHFATVLYKELTAVPYMAKFVVFAKMNDGREGRLRCYCMTDDKVDKTLEHHENFTEVARSRDIEVVEGMPLHVELSGNLVPVKKATQSRTFFFQSFRESRLAIPIKVRDSSREASGSLSFLRKAMKYEDLQHVLCHLNISIPPCTKGSSTEERRRTLTPLSLRSLSSTDRADQKMIDIAEQLGLSWAELARELQFGVDDINRIRVENPNSLLEQSIALLNLWVSREGKGVKIENLYTALRNIDRSEIISTLEGSGRQSRSMKGSWRYMDRDYSLSPSQMNGYASLQDELLSPASLHYMLPSPLRADQYWNEVAIMDAIPMAATEQDALMEMSDMQVWSSGLTPSLVTAEDSSLECSKAEDSDATSEGRFPGHLLTDAHVPDHMGSMDLVEDDTVDSDAMNGLIELLEQEEGQRPEGKMPASDHQLGTGEQNPESEVSFVSVQQKVQATIVPSPTVSHTVERSADRLRDWNAEGSFISCLQDLTEGSWQEGVTRRLGPAHTVAAGAQGQEQEQVLAPDVELMRVSSTEDSDWQPQHPAGGWPEEPDSRCFGQGNEVLHLPGEHVTEEQFTDEQGNIITKKVIRKVVHQLDLDTDDRKEHEDLILEGSLQEPQDLEAEADHFMYSILQRDGLGAKEEVQVRVPKLEVSGSRMGAQIVKRASLKRGKH

Target ORF information:

RefSeq Version	XM_015297396.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297396.1

ORF Insert Sequence:

ATGCCATGCT TGCATTACTT CAGGGCTGAT GCTGCGACCA GCTTCCTGAG AGCTGCAAGA 
TCTGGGAATC TGGACAGAGC CTTGGATCAT CTCAGGAATG GGGTAGATAT TAACACCTGT 
AACCAGAATG GGCTGAATGC CTTGCACCTG GCCTCCAAGG AGGGCCACAC GAAAATGGTG 
GTGGAGCTTC TGCACAAGGA GATTGTTTTG GAGACCACTA CCAAGAAGGG AAACACAGCC 
CTGCACATCG CTGCCCTCGC TGGACAGCAG GACGTGGTCC GGGAACTGGT GAACTATGGG 
GCCAATGTCA ATGCTCAGTC GCAGAAGGGC TTCACACCAC TCTACATGGC AGCGCAGGAA 
AACCACCTGG AAGTTGTCAA ATTCCTGCTG GAAAACGGAG CCAACCAGAA TGTAGCCACA 
GAGGATGGCT TCACACCACT AGCTGTGGCT CTGCAGCAGG GTCATGAGAA TGTGGTTGCT 
CACCTTATCA ACTATGGGAC GAAGGGTAAG GTCCGCCTGC CTGCCTTGCA CATTGCAGCT 
CGCAACGATG ACACCCGTAC AGCCGCCGTG CTGCTGCAGA ATGACCCCAA TGCTGACGTC 
CTCTCCAAGA CTGGATTTAC ACCTTTGCAC ATTGCAGCCC ACTATGAGAA TCTCAGCGTG 
GCCCAATTAT TGCTGAATCG TGGAGCCAGC GTCAACTTCA CACCCCAGAA TGGGATCACT 
CCTTTGCACA TAGCCTCTCG TCGAGGCAAC ATCATCATGG TACGGCTGCT GCTGGACCGT 
GGGGCCCAGA TAGAGACAAG GACCAAGGAT GAGTTGACGC CTCTCCACTG TGCAGCTCGC 
AATGGACATG TGCGAATTGC AGAGATCCTA CTGGACCATG GTGCTCCCAT TCAAGCCAAA 
ACAAAGAACG GTCTGTCACC GATCCACATG GCAGCACAGG GTGACCACCT GGACTGTGTG 
CGCCTACTCC TGCAGTACAG TGCTGAGATA GACGACATCA CCCTGGACCA CCTGACACCA 
CTGCATGTAG CTGCGCACTG TGGCCACCAC CGTGTGGCCA AGCTGCTGGT GGAGAAGGGA 
GCCAAGCCCA ACTCCAGAGC TCTGAATGGC TTCACGCCTC TCCACATTGC CTGCAAGAAG 
AACCACATTC GGGTGATGGA GTTGCTTCTG AAGACAGGAG CCTCCATTGA TGCTGTCACA 
GAGTCTGGCC TGACCCCCTT GCATGTGGCT GCCTTCATGG GGCACTTGCC CATTGTCAAA 
ACCTTGCTGC AGCGTGGAGC ATCTCCTAAT GTGTCCAATG TGAAAGTGGA GACTCCCCTG 
CACATGGCAG CCAGAGCTGG GCACATGGAT GTGGCCAAGT ATCTGATACA GAACAAGGCC 
AAAATCAACG CCAAGGCCAA GGATGACCAG ACTCCTCTGC ACTGTGCTGC ACGCATTGGC 
CACACCAGCA TGGTCCAACT CCTGCTGGAG AACAATGCCG ACCCCAACCT GGCCACTACA 
GCAGGGCACA CGCCTCTGCA CATCGCTGCC AGAGAGGGGC ATGTAGACAC AGCCTTGGCC 
CTGCTAGAGA AGGGAGCCTC TCAGACCTGC ATGACCAAGA AAGGATTTAC CCCTCTCCAC 
GTTGCAGCCA AGTATGGGAA GGTGGATGTG GCAGAGCTGC TGCTGGTGCA TGATGCTCAC 
CCCAATGCAG CGGGGAAGAA CGGCCTGACC CCACTGCACG TAGCTGTGCA CCACAACAAC 
CTGGAGATCG TCAAGCTTCT GCTTCCCAAG GGGAGCTCTC CACACAGCTC AGCCTGGAAC 
GGGTACACCC CTCTGCACAT TGCCGCCAAG CAGAACCAGA TGGAGGTGGC CAGCAGCTTG 
CTGCAGTATG GTGCTTCTGC AAATGCGGAG TCTGTTCAGG GAGTCACCCC TCTGCACCTG 
GCTTCTCAGG AAGGGCATAC AGACATGGTA GCACTGCTTT TCTCCAAGCA AGCCAATGGA 
AACTTAGGCA ACAAGAGTGG TCTGACTCCT CTCCATCTCG TGGCCCAAGA GGGACACGTA 
CCAGTGGCTG ATGTTCTGGT GAAACATGGA GTCACAGTGG ATGCGACAAC CAGGATGGGC 
TACACCTCAC TGCACATAGC CAGCCACTAT GGGAACATCA AGTTGGTGAA GTTTCTGCTG 
CAGCACCAGG CTGATGTCAA TGCTAAGACT AAGCTGGGCT ACACACCCCT GCACCAGGCG 
GCACAGCAGG GCCACACGGA TGTGGTGACC CTGCTGCTGA AGCATGGTGC CTCTCCCAAC 
GAGATCAGCA CAAATGGCAC CACTCCCCTG GCCATTGCGA AGCGCCTCGG TTACATTTCC 
GTCACAGATG TGCTCAAGAT AGTCACAGAG GAGACCACCA TCCCACCATT CAGTGACAAG 
TACCGCATGA GCTTCCCGGA GACCGTGGAT GAGATTCTGG ATGTGTCGGA GGATGAAGGC 
ACTGCTCATG TCACAGTAAT GGAGGAGGAG CTGGTCCCAC CGAAACCCAG AACACCTGAA 
CCCAAGGAGC AGGAGGGCAA GAGGGAGATG CTGGAGTTTG TGACCGTGAC AACACTGGAG 
CAAATGGAGG AATCTGCAGC TGTCCCACAG GTCCCCTGCG TGCCCCCTGA GACTGTGGTG 
ACCATAGCAG AGGAGTCTGA GCAGATGGGA CCTGTGGAGA CTGAGGCTGA GCAAGGCGGC 
CTGCTGCCTG CACCCTCGGT GTCCCCACAG GAGCCCTCCA AGGAGTTCGA TGAGGACTCC 
TTGATTCCCA GCAGCCCGGC CACCGAAACC TCGGACAACA TCAGCCCCGT GGCCAGCCCC 
GTTCACACAG GGTTCCTGGT GAGCTTCATG GTGGATGCGC GTGGCGGCTC CATGCGGGGC 
AGCCGGCACC ACGGGCTGCG CGTGGTCATC CCTCCCCGTG CCTGTGCTGC ACCCACCCGC 
ATCACTTGCC GCCTGGTGAA GCCTCAGAAG CTGCCTGCCC CCCCACCGCT GGCTGAGGAG 
GAGGGCCTGG CAAGCCGCAT CATTGCACTG GGGCCCGCTG GCGCCCAGTT CCTCAGCCCC 
GTCATTGTGG AGATTCCTCA CTTTGCCTCC TATGGACGTG GGGACCGTGA GCTGGTGGTG 
TTGCGCAGTG AGAATGGCTC TGTCTGGAAG GAGCACCGCA ACCGCTACGA GGAGAGCTAC 
ATGGACCAAC TGCTCAGTGG CATGGATGAG GAGCTGGAGA GCCAAGAGGA GCTGGAGAAG 
AAGAGGGTCT GCCGCATCAT CACCACTGAC TTTCCTCTCT ACTTCGTGGT CATGTCCCGG 
ATTTGCCAGG ACTGTGACAT GATTGGCCCC GAGGGAGGGT GCTTGAAAAG CACACTGGTG 
CCCATGGTGC AGGCCACCTT CCCAGACACC GCTGTCACCA AGAAAGTGAG GCTGGCTCTG 
CAGGCGCAGC CCGTGCCTGA TGAGCTGGTG ACCAAGCTGC TGGGGAACCA GGCAACCTTC 
AGCCCCATCG TCACAGTGGA GCCACGTCGG AGGAAGTTCC ACCGCCCCAT TGGCCTCCGC 
ATCCCTCTGC CCCCATCCTG GAAGGACAAT CCCCGGGACA GCGGCGAGGG TGACACCACC 
AGCCTGCGCC TGCTCTGTTC TGTGATAGGA GGGACAGCAC AAGCTCAGTG GGAAGACATC 
ACAGGCACCA CAAAGCTGGT CTATGAAAAA GAGTGTGCTA ACTTTACCAC CAATGTGTCT 
GCCAGGTTCT GGCTGGCTGA CTGCCCGCGC ACTGCTGAGG CTGTGCACTT TGCCACCGTG 
CTGTACAAGG AACTGACGGC TGTGCCCTAC ATGGCCAAAT TTGTGGTGTT TGCTAAGATG 
AACGATGGAC GGGAAGGCCG GCTGCGCTGC TACTGCATGA CTGATGACAA GGTGGACAAG 
ACTTTGGAGC ATCATGAGAA CTTCACTGAG GTGGCCCGCA GCAGGGACAT TGAGGTGGTG 
GAAGGAATGC CTCTGCATGT CGAGCTCTCA GGGAACCTGG TGCCCGTCAA GAAGGCCACG 
CAGTCACGCA CCTTCTTCTT CCAGTCCTTC CGGGAAAGCC GCCTCGCCAT CCCCATCAAG 
GTCCGGGACA GCAGCCGGGA AGCCAGTGGC TCCCTGTCCT TCCTGCGCAA GGCCATGAAG 
TATGAGGACT TGCAGCATGT GCTCTGCCAC CTCAACATCA GCATTCCACC CTGCACCAAG 
GGAAGCAGCA CTGAGGAGCG AAGGAGGACA CTGACACCTT TGTCTCTGCG CTCTCTGAGC 
AGCACCGACA GGGCAGACCA GAAGATGATC GACATTGCAG AGCAGCTTGG CCTCAGCTGG 
GCAGAGCTGG CACGTGAGCT GCAGTTTGGG GTGGATGACA TCAACAGGAT TCGTGTGGAG 
AACCCTAACT CCTTGCTGGA GCAGAGCATA GCCTTGCTTA ACCTCTGGGT CAGCCGCGAG 
GGCAAGGGTG TCAAAATTGA GAACCTGTAC ACAGCGCTGA GGAACATTGA CCGCAGCGAG 
ATCATCAGCA CGTTGGAGGG CTCCGGCCGG CAGAGCCGCA GCATGAAGGG CAGCTGGCGT 
TACATGGACA GGGACTATTC ACTCTCGCCC TCCCAGATGA ATGGTTACGC TTCGCTGCAG 
GACGAGCTGC TCTCCCCTGC CTCCCTGCAT TATATGCTGC CATCCCCTCT GCGTGCTGAC 
CAGTACTGGA ACGAGGTGGC CATCATGGAC GCCATCCCCA TGGCCGCCAC TGAGCAGGAC 
GCCCTGATGG AGATGTCTGA CATGCAGGTG TGGTCCTCAG GGCTCACCCC TTCACTGGTG 
ACGGCTGAGG ACTCCTCTCT GGAGTGCAGC AAGGCTGAGG ACTCGGATGC TACAAGCGAA 
GGTCGTTTCC CAGGGCACCT TCTGACAGAT GCACATGTCC CAGACCACAT GGGCTCTATG 
GACTTAGTTG AGGATGACAC AGTGGACTCA GACGCCATGA ACGGGCTGAT TGAACTGCTA 
GAGCAGGAGG AGGGGCAGAG GCCAGAGGGG AAGATGCCAG CCAGCGACCA CCAGCTAGGG 
ACTGGAGAGC AGAACCCAGA GAGTGAAGTC TCTTTTGTTT CAGTTCAACA GAAGGTACAA 
GCCACGATCG TGCCATCGCC AACCGTGAGC CACACAGTGG AGAGGAGTGC AGACAGGCTG 
AGGGACTGGA ATGCAGAAGG CTCCTTTATC TCCTGCCTAC AGGACCTGAC AGAGGGCTCC 
TGGCAGGAGG GGGTCACCCG ACGGCTGGGT CCAGCACACA CCGTGGCTGC TGGGGCGCAG 
GGCCAGGAGC AAGAGCAGGT CCTGGCGCCG GACGTGGAGC TGATGCGGGT CAGCTCCACA 
GAGGACAGCG ACTGGCAGCC CCAGCACCCC GCGGGCGGCT GGCCAGAGGA GCCTGACAGC 
CGCTGCTTTG GGCAGGGCAA CGAAGTCCTC CATCTCCCTG GGGAGCATGT GACTGAGGAG 
CAGTTCACAG ATGAACAAGG CAATATAATC ACCAAGAAGG TCATCCGGAA AGTGGTGCAT 
CAGTTGGACC TCGACACGGA TGACAGGAAG GAGCATGAGG ACTTGATTCT GGAGGGCTCC 
CTGCAGGAGC CCCAAGACCT GGAGGCAGAG GCTGATCACT TCATGTACTC CATCCTACAG 
CGGGACGGTC TGGGGGCCAA GGAGGAGGTG CAAGTGCGTG TCCCAAAACT GGAAGTCTCC 
GGGAGCAGGA TGGGGGCTCA GATAGTGAAA CGAGCCAGTC TGAAAAGGGG GAAGCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK1 ( XM_015297397.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152883.1 Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X8, mRNA.

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CloneID	OGa45392
Clone ID Related Accession (Same CDS sequence)	XM_015297397.1
Accession Version	XM_015297397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ankyrin-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455999.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGAGAG GGCATGCACC AACCACTGTG GACCCCTTGC CTTCTTGTTG CAAGCAAGGA 
TCTCCTGAAG GGATGCTGGA GCAGGAGTCT GCTGTGCAGG CTGATGCTGC GACCAGCTTC 
CTGAGAGCTG CAAGATCTGG GAATCTGGAC AGAGCCTTGG ATCATCTCAG GAATGGGGTA 
GATATTAACA CCTGTAACCA GAATGGGCTG AATGCCTTGC ACCTGGCCTC CAAGGAGGGC 
CACACGAAAA TGGTGGTGGA GCTTCTGCAC AAGGAGATTG TTTTGGAGAC CACTACCAAG 
AAGGGAAACA CAGCCCTGCA CATCGCTGCC CTCGCTGGAC AGCAGGACGT GGTCCGGGAA 
CTGGTGAACT ATGGGGCCAA TGTCAATGCT CAGTCGCAGA AGGGCTTCAC ACCACTCTAC 
ATGGCAGCGC AGGAAAACCA CCTGGAAGTT GTCAAATTCC TGCTGGAAAA CGGAGCCAAC 
CAGAATGTAG CCACAGAGGA TGGCTTCACA CCACTAGCTG TGGCTCTGCA GCAGGGTCAT 
GAGAATGTGG TTGCTCACCT TATCAACTAT GGGACGAAGG GTAAGGTCCG CCTGCCTGCC 
TTGCACATTG CAGCTCGCAA CGATGACACC CGTACAGCCG CCGTGCTGCT GCAGAATGAC 
CCCAATGCTG ACGTCCTCTC CAAGACTGGA TTTACACCTT TGCACATTGC AGCCCACTAT 
GAGAATCTCA GCGTGGCCCA ATTATTGCTG AATCGTGGAG CCAGCGTCAA CTTCACACCC 
CAGAATGGGA TCACTCCTTT GCACATAGCC TCTCGTCGAG GCAACATCAT CATGGTACGG 
CTGCTGCTGG ACCGTGGGGC CCAGATAGAG ACAAGGACCA AGGATGAGTT GACGCCTCTC 
CACTGTGCAG CTCGCAATGG ACATGTGCGA ATTGCAGAGA TCCTACTGGA CCATGGTGCT 
CCCATTCAAG CCAAAACAAA GAACGGTCTG TCACCGATCC ACATGGCAGC ACAGGGTGAC 
CACCTGGACT GTGTGCGCCT ACTCCTGCAG TACAGTGCTG AGATAGACGA CATCACCCTG 
GACCACCTGA CACCACTGCA TGTAGCTGCG CACTGTGGCC ACCACCGTGT GGCCAAGCTG 
CTGGTGGAGA AGGGAGCCAA GCCCAACTCC AGAGCTCTGA ATGGCTTCAC GCCTCTCCAC 
ATTGCCTGCA AGAAGAACCA CATTCGGGTG ATGGAGTTGC TTCTGAAGAC AGGAGCCTCC 
ATTGATGCTG TCACAGAGTC TGGCCTGACC CCCTTGCATG TGGCTGCCTT CATGGGGCAC 
TTGCCCATTG TCAAAACCTT GCTGCAGCGT GGAGCATCTC CTAATGTGTC CAATGTGAAA 
GTGGAGACTC CCCTGCACAT GGCAGCCAGA GCTGGGCACA TGGATGTGGC CAAGTATCTG 
ATACAGAACA AGGCCAAAAT CAACGCCAAG GCCAAGGATG ACCAGACTCC TCTGCACTGT 
GCTGCACGCA TTGGCCACAC CAGCATGGTC CAACTCCTGC TGGAGAACAA TGCCGACCCC 
AACCTGGCCA CTACAGCAGG GCACACGCCT CTGCACATCG CTGCCAGAGA GGGGCATGTA 
GACACAGCCT TGGCCCTGCT AGAGAAGGGA GCCTCTCAGA CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TCCACGTTGC AGCCAAGTAT GGGAAGGTGG ATGTGGCAGA GCTGCTGCTG 
GTGCATGATG CTCACCCCAA TGCAGCGGGG AAGAACGGCC TGACCCCACT GCACGTAGCT 
GTGCACCACA ACAACCTGGA GATCGTCAAG CTTCTGCTTC CCAAGGGGAG CTCTCCACAC 
AGCTCAGCCT GGAACGGGTA CACCCCTCTG CACATTGCCG CCAAGCAGAA CCAGATGGAG 
GTGGCCAGCA GCTTGCTGCA GTATGGTGCT TCTGCAAATG CGGAGTCTGT TCAGGGAGTC 
ACCCCTCTGC ACCTGGCTTC TCAGGAAGGG CATACAGACA TGGTAGCACT GCTTTTCTCC 
AAGCAAGCCA ATGGAAACTT AGGCAACAAG AGTGGTCTGA CTCCTCTCCA TCTCGTGGCC 
CAAGAGGGAC ACGTACCAGT GGCTGATGTT CTGGTGAAAC ATGGAGTCAC AGTGGATGCG 
ACAACCAGGA TGGGCTACAC CTCACTGCAC ATAGCCAGCC ACTATGGGAA CATCAAGTTG 
GTGAAGTTTC TGCTGCAGCA CCAGGCTGAT GTCAATGCTA AGACTAAGCT GGGCTACACA 
CCCCTGCACC AGGCGGCACA GCAGGGCCAC ACGGATGTGG TGACCCTGCT GCTGAAGCAT 
GGTGCCTCTC CCAACGAGAT CAGCACAAAT GGCACCACTC CCCTGGCCAT TGCGAAGCGC 
CTCGGTTACA TTTCCGTCAC AGATGTGCTC AAGATAGTCA CAGAGGAGAC CACCATCCCA 
CCATTCAGTG ACAAGTACCG CATGAGCTTC CCGGAGACCG TGGATGAGAT TCTGGATGTG 
TCGGAGGATG AAGGCACTGC TCATGTCACA GTAATGGAGG AGGAGCTGGT CCCACCGAAA 
CCCAGAACAC CTGAACCCAA GGAGCAGGAG GGCAAGAGGG AGATGCTGGA GTTTGTGACC 
GTGACAACAC TGGAGCAAAT GGAGGAATCT GCAGCTGTCC CACAGGTCCC CTGCGTGCCC 
CCTGAGACTG TGGTGACCAT AGCAGAGGAG TCTGAGCAGA TGGGACCTGT GGAGACTGAG 
GCTGAGCAAG GCGGCCTGCT GCCTGCACCC TCGGTGTCCC CACAGGAGCC CTCCAAGGAG 
TTCGATGAGG ACTCCTTGAT TCCCAGCAGC CCGGCCACCG AAACCTCGGA CAACATCAGC 
CCCGTGGCCA GCCCCGTTCA CACAGGGTTC CTGGTGAGCT TCATGGTGGA TGCGCGTGGC 
GGCTCCATGC GGGGCAGCCG GCACCACGGG CTGCGCGTGG TCATCCCTCC CCGTGCCTGT 
GCTGCACCCA CCCGCATCAC TTGCCGCCTG GTGAAGCCTC AGAAGCTGCC TGCCCCCCCA 
CCGCTGGCTG AGGAGGAGGG CCTGGCAAGC CGCATCATTG CACTGGGGCC CGCTGGCGCC 
CAGTTCCTCA GCCCCGTCAT TGTGGAGATT CCTCACTTTG CCTCCTATGG ACGTGGGGAC 
CGTGAGCTGG TGGTGTTGCG CAGTGAGAAT GGCTCTGTCT GGAAGGAGCA CCGCAACCGC 
TACGAGGAGA GCTACATGGA CCAACTGCTC AGTGGCATGG ATGAGGAGCT GGAGAGCCAA 
GAGGAGCTGG AGAAGAAGAG GGTCTGCCGC ATCATCACCA CTGACTTTCC TCTCTACTTC 
GTGGTCATGT CCCGGATTTG CCAGGACTGT GACATGATTG GCCCCGAGGG AGGGTGCTTG 
AAAAGCACAC TGGTGCCCAT GGTGCAGGCC ACCTTCCCAG ACACCGCTGT CACCAAGAAA 
GTGAGGCTGG CTCTGCAGGC GCAGCCCGTG CCTGATGAGC TGGTGACCAA GCTGCTGGGG 
AACCAGGCAA CCTTCAGCCC CATCGTCACA GTGGAGCCAC GTCGGAGGAA GTTCCACCGC 
CCCATTGGCC TCCGCATCCC TCTGCCCCCA TCCTGGAAGG ACAATCCCCG GGACAGCGGC 
GAGGGTGACA CCACCAGCCT GCGCCTGCTC TGTTCTGTGA TAGGAGGGAC AGCACAAGCT 
CAGTGGGAAG ACATCACAGG CACCACAAAG CTGGTCTATG AAAAAGAGTG TGCTAACTTT 
ACCACCAATG TGTCTGCCAG GTTCTGGCTG GCTGACTGCC CGCGCACTGC TGAGGCTGTG 
CACTTTGCCA CCGTGCTGTA CAAGGAACTG ACGGCTGTGC CCTACATGGC CAAATTTGTG 
GTGTTTGCTA AGATGAACGA TGGACGGGAA GGCCGGCTGC GCTGCTACTG CATGACTGAT 
GACAAGGTGG ACAAGACTTT GGAGCATCAT GAGAACTTCA CTGAGGTGGC CCGCAGCAGG 
GACATTGAGG TGGTGGAAGG AATGCCTCTG CATGTCGAGC TCTCAGGGAA CCTGGTGCCC 
GTCAAGAAGG CCACGCAGTC ACGCACCTTC TTCTTCCAGT CCTTCCGGGA AAGCCGCCTC 
GCCATCCCCA TCAAGGTCCG GGACAGCAGC CGGGAAGCCA GTGGCTCCCT GTCCTTCCTG 
CGCAAGGCCA TGAAGTATGA GGACTTGCAG CATGTGCTCT GCCACCTCAA CATCAGCATT 
CCACCCTGCA CCAAGGGAAG CAGCACTGAG GAGCGAAGGA GGACACTGAC ACCTTTGTCT 
CTGCGCTCTC TGAGCAGCAC CGACAGGGCA GACCAGAAGA TGATCGACAT TGCAGAGCAG 
CTTGGCCTCA GCTGGGCAGA GCTGGCACGT GAGCTGCAGT TTGGGGTGGA TGACATCAAC 
AGGATTCGTG TGGAGAACCC TAACTCCTTG CTGGAGCAGA GCATAGCCTT GCTTAACCTC 
TGGGTCAGCC GCGAGGGCAA GGGTGTCAAA ATTGAGAACC TGTACACAGC GCTGAGGAAC 
ATTGACCGCA GCGAGATCAT CAGCACGTTG GAGGGCTCCG GCCGGCAGAG CCGCAGCATG 
AAGGGCAGCT GGCGTTACAT GGACAGGGAC TATTCACTCT CGCCCTCCCA GATGAATGTT 
CAACAGAAGG TACAAGCCAC GATCGTGCCA TCGCCAACCG TGAGCCACAC AGTGGAGAGG 
AGTGCAGACA GGCTGAGGGA CTGGAATGCA GAAGGCTCCT TTATCTCCTG CCTACAGGAC 
CTGACAGAGG GCTCCTGGCA GGAGGGGGTC ACCCGACGGC TGGGTCCAGC ACACACCGTG 
GCTGCTGGGG CGCAGGGCCA GGAGCAAGAG CAGGTCCTGG CGCCGGACGT GGAGCTGATG 
CGGGTCAGCT CCACAGAGGA CAGCGACTGG CAGCCCCAGC ACCCCGCGGG CGGCTGGCCA 
GAGGAGCCTG ACAGCCGCTG CTTTGGGCAG GGCAACGAAG TCCTCCATCT CCCTGGGGAG 
CATGTGACTG AGGAGCAGTT CACAGATGAA CAAGGCAATA TAATCACCAA GAAGGTCATC 
CGGAAAGTGG TGCATCAGTT GGACCTCGAC ACGGATGACA GGAAGGAGCA TGAGGACTTG 
ATTCTGGAGG GCTCCCTGCA GGAGCCCCAA GACCTGGAGG CAGAGGCTGA TCACTTCATG 
TACTCCATCC TACAGCGGGA CGGTCTGGGG GCCAAGGAGG AGGTGCAAGT GCGTGTCCCA 
AAACTGGAAG TCTCCGGGAG CAGGATGGGG GCTCAGATAG TGAAACGAGC CAGTCTGAAA 
AGGGGGAAGC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152883.1
CDS	881..6355
Translation	MAQAAKQLKKIKDIEAQALQEQKEKEESNRKRRNRSRDRKKKRGHAPTTVDPLPSCCKQGSPEGMLEQESAVQADAATSFLRAARSGNLDRALDHLRNGVDINTCNQNGLNALHLASKEGHTKMVVELLHKEIVLETTTKKGNTALHIAALAGQQDVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLSVAQLLLNRGASVNFTPQNGITPLHIASRRGNIIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRIAEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYSAEIDDITLDHLTPLHVAAHCGHHRVAKLLVEKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVAAFMGHLPIVKTLLQRGASPNVSNVKVETPLHMAARAGHMDVAKYLIQNKAKINAKAKDDQTPLHCAARIGHTSMVQLLLENNADPNLATTAGHTPLHIAAREGHVDTALALLEKGASQTCMTKKGFTPLHVAAKYGKVDVAELLLVHDAHPNAAGKNGLTPLHVAVHHNNLEIVKLLLPKGSSPHSSAWNGYTPLHIAAKQNQMEVASSLLQYGASANAESVQGVTPLHLASQEGHTDMVALLFSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLVKHGVTVDATTRMGYTSLHIASHYGNIKLVKFLLQHQADVNAKTKLGYTPLHQAAQQGHTDVVTLLLKHGASPNEISTNGTTPLAIAKRLGYISVTDVLKIVTEETTIPPFSDKYRMSFPETVDEILDVSEDEGTAHVTVMEEELVPPKPRTPEPKEQEGKREMLEFVTVTTLEQMEESAAVPQVPCVPPETVVTIAEESEQMGPVETEAEQGGLLPAPSVSPQEPSKEFDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHHGLRVVIPPRACAAPTRITCRLVKPQKLPAPPPLAEEEGLASRIIALGPAGAQFLSPVIVEIPHFASYGRGDRELVVLRSENGSVWKEHRNRYEESYMDQLLSGMDEELESQEELEKKRVCRIITTDFPLYFVVMSRICQDCDMIGPEGGCLKSTLVPMVQATFPDTAVTKKVRLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWKDNPRDSGEGDTTSLRLLCSVIGGTAQAQWEDITGTTKLVYEKECANFTTNVSARFWLADCPRTAEAVHFATVLYKELTAVPYMAKFVVFAKMNDGREGRLRCYCMTDDKVDKTLEHHENFTEVARSRDIEVVEGMPLHVELSGNLVPVKKATQSRTFFFQSFRESRLAIPIKVRDSSREASGSLSFLRKAMKYEDLQHVLCHLNISIPPCTKGSSTEERRRTLTPLSLRSLSSTDRADQKMIDIAEQLGLSWAELARELQFGVDDINRIRVENPNSLLEQSIALLNLWVSREGKGVKIENLYTALRNIDRSEIISTLEGSGRQSRSMKGSWRYMDRDYSLSPSQMNVQQKVQATIVPSPTVSHTVERSADRLRDWNAEGSFISCLQDLTEGSWQEGVTRRLGPAHTVAAGAQGQEQEQVLAPDVELMRVSSTEDSDWQPQHPAGGWPEEPDSRCFGQGNEVLHLPGEHVTEEQFTDEQGNIITKKVIRKVVHQLDLDTDDRKEHEDLILEGSLQEPQDLEAEADHFMYSILQRDGLGAKEEVQVRVPKLEVSGSRMGAQIVKRASLKRGKH

Target ORF information:

RefSeq Version	XM_015297397.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297397.1

ORF Insert Sequence:

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGAGAG GGCATGCACC AACCACTGTG GACCCCTTGC CTTCTTGTTG CAAGCAAGGA 
TCTCCTGAAG GGATGCTGGA GCAGGAGTCT GCTGTGCAGG CTGATGCTGC GACCAGCTTC 
CTGAGAGCTG CAAGATCTGG GAATCTGGAC AGAGCCTTGG ATCATCTCAG GAATGGGGTA 
GATATTAACA CCTGTAACCA GAATGGGCTG AATGCCTTGC ACCTGGCCTC CAAGGAGGGC 
CACACGAAAA TGGTGGTGGA GCTTCTGCAC AAGGAGATTG TTTTGGAGAC CACTACCAAG 
AAGGGAAACA CAGCCCTGCA CATCGCTGCC CTCGCTGGAC AGCAGGACGT GGTCCGGGAA 
CTGGTGAACT ATGGGGCCAA TGTCAATGCT CAGTCGCAGA AGGGCTTCAC ACCACTCTAC 
ATGGCAGCGC AGGAAAACCA CCTGGAAGTT GTCAAATTCC TGCTGGAAAA CGGAGCCAAC 
CAGAATGTAG CCACAGAGGA TGGCTTCACA CCACTAGCTG TGGCTCTGCA GCAGGGTCAT 
GAGAATGTGG TTGCTCACCT TATCAACTAT GGGACGAAGG GTAAGGTCCG CCTGCCTGCC 
TTGCACATTG CAGCTCGCAA CGATGACACC CGTACAGCCG CCGTGCTGCT GCAGAATGAC 
CCCAATGCTG ACGTCCTCTC CAAGACTGGA TTTACACCTT TGCACATTGC AGCCCACTAT 
GAGAATCTCA GCGTGGCCCA ATTATTGCTG AATCGTGGAG CCAGCGTCAA CTTCACACCC 
CAGAATGGGA TCACTCCTTT GCACATAGCC TCTCGTCGAG GCAACATCAT CATGGTACGG 
CTGCTGCTGG ACCGTGGGGC CCAGATAGAG ACAAGGACCA AGGATGAGTT GACGCCTCTC 
CACTGTGCAG CTCGCAATGG ACATGTGCGA ATTGCAGAGA TCCTACTGGA CCATGGTGCT 
CCCATTCAAG CCAAAACAAA GAACGGTCTG TCACCGATCC ACATGGCAGC ACAGGGTGAC 
CACCTGGACT GTGTGCGCCT ACTCCTGCAG TACAGTGCTG AGATAGACGA CATCACCCTG 
GACCACCTGA CACCACTGCA TGTAGCTGCG CACTGTGGCC ACCACCGTGT GGCCAAGCTG 
CTGGTGGAGA AGGGAGCCAA GCCCAACTCC AGAGCTCTGA ATGGCTTCAC GCCTCTCCAC 
ATTGCCTGCA AGAAGAACCA CATTCGGGTG ATGGAGTTGC TTCTGAAGAC AGGAGCCTCC 
ATTGATGCTG TCACAGAGTC TGGCCTGACC CCCTTGCATG TGGCTGCCTT CATGGGGCAC 
TTGCCCATTG TCAAAACCTT GCTGCAGCGT GGAGCATCTC CTAATGTGTC CAATGTGAAA 
GTGGAGACTC CCCTGCACAT GGCAGCCAGA GCTGGGCACA TGGATGTGGC CAAGTATCTG 
ATACAGAACA AGGCCAAAAT CAACGCCAAG GCCAAGGATG ACCAGACTCC TCTGCACTGT 
GCTGCACGCA TTGGCCACAC CAGCATGGTC CAACTCCTGC TGGAGAACAA TGCCGACCCC 
AACCTGGCCA CTACAGCAGG GCACACGCCT CTGCACATCG CTGCCAGAGA GGGGCATGTA 
GACACAGCCT TGGCCCTGCT AGAGAAGGGA GCCTCTCAGA CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TCCACGTTGC AGCCAAGTAT GGGAAGGTGG ATGTGGCAGA GCTGCTGCTG 
GTGCATGATG CTCACCCCAA TGCAGCGGGG AAGAACGGCC TGACCCCACT GCACGTAGCT 
GTGCACCACA ACAACCTGGA GATCGTCAAG CTTCTGCTTC CCAAGGGGAG CTCTCCACAC 
AGCTCAGCCT GGAACGGGTA CACCCCTCTG CACATTGCCG CCAAGCAGAA CCAGATGGAG 
GTGGCCAGCA GCTTGCTGCA GTATGGTGCT TCTGCAAATG CGGAGTCTGT TCAGGGAGTC 
ACCCCTCTGC ACCTGGCTTC TCAGGAAGGG CATACAGACA TGGTAGCACT GCTTTTCTCC 
AAGCAAGCCA ATGGAAACTT AGGCAACAAG AGTGGTCTGA CTCCTCTCCA TCTCGTGGCC 
CAAGAGGGAC ACGTACCAGT GGCTGATGTT CTGGTGAAAC ATGGAGTCAC AGTGGATGCG 
ACAACCAGGA TGGGCTACAC CTCACTGCAC ATAGCCAGCC ACTATGGGAA CATCAAGTTG 
GTGAAGTTTC TGCTGCAGCA CCAGGCTGAT GTCAATGCTA AGACTAAGCT GGGCTACACA 
CCCCTGCACC AGGCGGCACA GCAGGGCCAC ACGGATGTGG TGACCCTGCT GCTGAAGCAT 
GGTGCCTCTC CCAACGAGAT CAGCACAAAT GGCACCACTC CCCTGGCCAT TGCGAAGCGC 
CTCGGTTACA TTTCCGTCAC AGATGTGCTC AAGATAGTCA CAGAGGAGAC CACCATCCCA 
CCATTCAGTG ACAAGTACCG CATGAGCTTC CCGGAGACCG TGGATGAGAT TCTGGATGTG 
TCGGAGGATG AAGGCACTGC TCATGTCACA GTAATGGAGG AGGAGCTGGT CCCACCGAAA 
CCCAGAACAC CTGAACCCAA GGAGCAGGAG GGCAAGAGGG AGATGCTGGA GTTTGTGACC 
GTGACAACAC TGGAGCAAAT GGAGGAATCT GCAGCTGTCC CACAGGTCCC CTGCGTGCCC 
CCTGAGACTG TGGTGACCAT AGCAGAGGAG TCTGAGCAGA TGGGACCTGT GGAGACTGAG 
GCTGAGCAAG GCGGCCTGCT GCCTGCACCC TCGGTGTCCC CACAGGAGCC CTCCAAGGAG 
TTCGATGAGG ACTCCTTGAT TCCCAGCAGC CCGGCCACCG AAACCTCGGA CAACATCAGC 
CCCGTGGCCA GCCCCGTTCA CACAGGGTTC CTGGTGAGCT TCATGGTGGA TGCGCGTGGC 
GGCTCCATGC GGGGCAGCCG GCACCACGGG CTGCGCGTGG TCATCCCTCC CCGTGCCTGT 
GCTGCACCCA CCCGCATCAC TTGCCGCCTG GTGAAGCCTC AGAAGCTGCC TGCCCCCCCA 
CCGCTGGCTG AGGAGGAGGG CCTGGCAAGC CGCATCATTG CACTGGGGCC CGCTGGCGCC 
CAGTTCCTCA GCCCCGTCAT TGTGGAGATT CCTCACTTTG CCTCCTATGG ACGTGGGGAC 
CGTGAGCTGG TGGTGTTGCG CAGTGAGAAT GGCTCTGTCT GGAAGGAGCA CCGCAACCGC 
TACGAGGAGA GCTACATGGA CCAACTGCTC AGTGGCATGG ATGAGGAGCT GGAGAGCCAA 
GAGGAGCTGG AGAAGAAGAG GGTCTGCCGC ATCATCACCA CTGACTTTCC TCTCTACTTC 
GTGGTCATGT CCCGGATTTG CCAGGACTGT GACATGATTG GCCCCGAGGG AGGGTGCTTG 
AAAAGCACAC TGGTGCCCAT GGTGCAGGCC ACCTTCCCAG ACACCGCTGT CACCAAGAAA 
GTGAGGCTGG CTCTGCAGGC GCAGCCCGTG CCTGATGAGC TGGTGACCAA GCTGCTGGGG 
AACCAGGCAA CCTTCAGCCC CATCGTCACA GTGGAGCCAC GTCGGAGGAA GTTCCACCGC 
CCCATTGGCC TCCGCATCCC TCTGCCCCCA TCCTGGAAGG ACAATCCCCG GGACAGCGGC 
GAGGGTGACA CCACCAGCCT GCGCCTGCTC TGTTCTGTGA TAGGAGGGAC AGCACAAGCT 
CAGTGGGAAG ACATCACAGG CACCACAAAG CTGGTCTATG AAAAAGAGTG TGCTAACTTT 
ACCACCAATG TGTCTGCCAG GTTCTGGCTG GCTGACTGCC CGCGCACTGC TGAGGCTGTG 
CACTTTGCCA CCGTGCTGTA CAAGGAACTG ACGGCTGTGC CCTACATGGC CAAATTTGTG 
GTGTTTGCTA AGATGAACGA TGGACGGGAA GGCCGGCTGC GCTGCTACTG CATGACTGAT 
GACAAGGTGG ACAAGACTTT GGAGCATCAT GAGAACTTCA CTGAGGTGGC CCGCAGCAGG 
GACATTGAGG TGGTGGAAGG AATGCCTCTG CATGTCGAGC TCTCAGGGAA CCTGGTGCCC 
GTCAAGAAGG CCACGCAGTC ACGCACCTTC TTCTTCCAGT CCTTCCGGGA AAGCCGCCTC 
GCCATCCCCA TCAAGGTCCG GGACAGCAGC CGGGAAGCCA GTGGCTCCCT GTCCTTCCTG 
CGCAAGGCCA TGAAGTATGA GGACTTGCAG CATGTGCTCT GCCACCTCAA CATCAGCATT 
CCACCCTGCA CCAAGGGAAG CAGCACTGAG GAGCGAAGGA GGACACTGAC ACCTTTGTCT 
CTGCGCTCTC TGAGCAGCAC CGACAGGGCA GACCAGAAGA TGATCGACAT TGCAGAGCAG 
CTTGGCCTCA GCTGGGCAGA GCTGGCACGT GAGCTGCAGT TTGGGGTGGA TGACATCAAC 
AGGATTCGTG TGGAGAACCC TAACTCCTTG CTGGAGCAGA GCATAGCCTT GCTTAACCTC 
TGGGTCAGCC GCGAGGGCAA GGGTGTCAAA ATTGAGAACC TGTACACAGC GCTGAGGAAC 
ATTGACCGCA GCGAGATCAT CAGCACGTTG GAGGGCTCCG GCCGGCAGAG CCGCAGCATG 
AAGGGCAGCT GGCGTTACAT GGACAGGGAC TATTCACTCT CGCCCTCCCA GATGAATGTT 
CAACAGAAGG TACAAGCCAC GATCGTGCCA TCGCCAACCG TGAGCCACAC AGTGGAGAGG 
AGTGCAGACA GGCTGAGGGA CTGGAATGCA GAAGGCTCCT TTATCTCCTG CCTACAGGAC 
CTGACAGAGG GCTCCTGGCA GGAGGGGGTC ACCCGACGGC TGGGTCCAGC ACACACCGTG 
GCTGCTGGGG CGCAGGGCCA GGAGCAAGAG CAGGTCCTGG CGCCGGACGT GGAGCTGATG 
CGGGTCAGCT CCACAGAGGA CAGCGACTGG CAGCCCCAGC ACCCCGCGGG CGGCTGGCCA 
GAGGAGCCTG ACAGCCGCTG CTTTGGGCAG GGCAACGAAG TCCTCCATCT CCCTGGGGAG 
CATGTGACTG AGGAGCAGTT CACAGATGAA CAAGGCAATA TAATCACCAA GAAGGTCATC 
CGGAAAGTGG TGCATCAGTT GGACCTCGAC ACGGATGACA GGAAGGAGCA TGAGGACTTG 
ATTCTGGAGG GCTCCCTGCA GGAGCCCCAA GACCTGGAGG CAGAGGCTGA TCACTTCATG 
TACTCCATCC TACAGCGGGA CGGTCTGGGG GCCAAGGAGG AGGTGCAAGT GCGTGTCCCA 
AAACTGGAAG TCTCCGGGAG CAGGATGGGG GCTCAGATAG TGAAACGAGC CAGTCTGAAA 
AGGGGGAAGC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK1 ( XM_015297398.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152884.1 Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X9, mRNA.

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CloneID	OGa45393
Clone ID Related Accession (Same CDS sequence)	XM_015297398.1
Accession Version	XM_015297398.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ankyrin-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455999.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGAGAG GGCATGCACC AACCACTGTG GACCCCTTGC CTTCTTGTTG CAAGCAAGGA 
TCTCCTGAAG GGATGCTGGA GCAGGAGTCT GCTGTGCAGG CTGATGCTGC GACCAGCTTC 
CTGAGAGCTG CAAGATCTGG GAATCTGGAC AGAGCCTTGG ATCATCTCAG GAATGGGGTA 
GATATTAACA CCTGTAACCA GAATGGGCTG AATGCCTTGC ACCTGGCCTC CAAGGAGGGC 
CACACGAAAA TGGTGGTGGA GCTTCTGCAC AAGGAGATTG TTTTGGAGAC CACTACCAAG 
AAGGGAAACA CAGCCCTGCA CATCGCTGCC CTCGCTGGAC AGCAGGACGT GGTCCGGGAA 
CTGGTGAACT ATGGGGCCAA TGTCAATGCT CAGTCGCAGA AGGGCTTCAC ACCACTCTAC 
ATGGCAGCGC AGGAAAACCA CCTGGAAGTT GTCAAATTCC TGCTGGAAAA CGGAGCCAAC 
CAGAATGTAG CCACAGAGGA TGGCTTCACA CCACTAGCTG TGGCTCTGCA GCAGGGTCAT 
GAGAATGTGG TTGCTCACCT TATCAACTAT GGGACGAAGG GTAAGGTCCG CCTGCCTGCC 
TTGCACATTG CAGCTCGCAA CGATGACACC CGTACAGCCG CCGTGCTGCT GCAGAATGAC 
CCCAATGCTG ACGTCCTCTC CAAGACTGGA TTTACACCTT TGCACATTGC AGCCCACTAT 
GAGAATCTCA GCGTGGCCCA ATTATTGCTG AATCGTGGAG CCAGCGTCAA CTTCACACCC 
CAGAATGGGA TCACTCCTTT GCACATAGCC TCTCGTCGAG GCAACATCAT CATGGTACGG 
CTGCTGCTGG ACCGTGGGGC CCAGATAGAG ACAAGGACCA AGGATGAGTT GACGCCTCTC 
CACTGTGCAG CTCGCAATGG ACATGTGCGA ATTGCAGAGA TCCTACTGGA CCATGGTGCT 
CCCATTCAAG CCAAAACAAA GAACGGTCTG TCACCGATCC ACATGGCAGC ACAGGGTGAC 
CACCTGGACT GTGTGCGCCT ACTCCTGCAG TACAGTGCTG AGATAGACGA CATCACCCTG 
GACCACCTGA CACCACTGCA TGTAGCTGCG CACTGTGGCC ACCACCGTGT GGCCAAGCTG 
CTGGTGGAGA AGGGAGCCAA GCCCAACTCC AGAGCTCTGA ATGGCTTCAC GCCTCTCCAC 
ATTGCCTGCA AGAAGAACCA CATTCGGGTG ATGGAGTTGC TTCTGAAGAC AGGAGCCTCC 
ATTGATGCTG TCACAGAGTC TGGCCTGACC CCCTTGCATG TGGCTGCCTT CATGGGGCAC 
TTGCCCATTG TCAAAACCTT GCTGCAGCGT GGAGCATCTC CTAATGTGTC CAATGTGAAA 
GTGGAGACTC CCCTGCACAT GGCAGCCAGA GCTGGGCACA TGGATGTGGC CAAGTATCTG 
ATACAGAACA AGGCCAAAAT CAACGCCAAG GCCAAGGATG ACCAGACTCC TCTGCACTGT 
GCTGCACGCA TTGGCCACAC CAGCATGGTC CAACTCCTGC TGGAGAACAA TGCCGACCCC 
AACCTGGCCA CTACAGCAGG GCACACGCCT CTGCACATCG CTGCCAGAGA GGGGCATGTA 
GACACAGCCT TGGCCCTGCT AGAGAAGGGA GCCTCTCAGA CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TCCACGTTGC AGCCAAGTAT GGGAAGGTGG ATGTGGCAGA GCTGCTGCTG 
GTGCATGATG CTCACCCCAA TGCAGCGGGG AAGAACGGCC TGACCCCACT GCACGTAGCT 
GTGCACCACA ACAACCTGGA GATCGTCAAG CTTCTGCTTC CCAAGGGGAG CTCTCCACAC 
AGCTCAGCCT GGAACGGGTA CACCCCTCTG CACATTGCCG CCAAGCAGAA CCAGATGGAG 
GTGGCCAGCA GCTTGCTGCA GTATGGTGCT TCTGCAAATG CGGAGTCTGT TCAGGGAGTC 
ACCCCTCTGC ACCTGGCTTC TCAGGAAGGG CATACAGACA TGGTAGCACT GCTTTTCTCC 
AAGCAAGCCA ATGGAAACTT AGGCAACAAG AGTGGTCTGA CTCCTCTCCA TCTCGTGGCC 
CAAGAGGGAC ACGTACCAGT GGCTGATGTT CTGGTGAAAC ATGGAGTCAC AGTGGATGCG 
ACAACCAGGA TGGGCTACAC CTCACTGCAC ATAGCCAGCC ACTATGGGAA CATCAAGTTG 
GTGAAGTTTC TGCTGCAGCA CCAGGCTGAT GTCAATGCTA AGACTAAGCT GGGCTACACA 
CCCCTGCACC AGGCGGCACA GCAGGGCCAC ACGGATGTGG TGACCCTGCT GCTGAAGCAT 
GGTGCCTCTC CCAACGAGAT CAGCACAAAT GGCACCACTC CCCTGGCCAT TGCGAAGCGC 
CTCGGTTACA TTTCCGTCAC AGATGTGCTC AAGATAGTCA CAGAGGAGAC CACCATCCCA 
CCATTCAGTG ACAAGTACCG CATGAGCTTC CCGGAGACCG TGGATGAGAT TCTGGATGTG 
TCGGAGGATG AAGGCACTGC TCATGTCACA GTAATGGAGG AGGAGCTGGT CCCACCGAAA 
CCCAGAACAC CTGAACCCAA GGAGCAGGAG GGCAAGAGGG AGATGCTGGA GTTTGTGACC 
GTGACAACAC TGGAGCAAAT GGAGGAATCT GCAGCTGTCC CACAGGTCCC CTGCGTGCCC 
CCTGAGACTG TGGTGACCAT AGCAGAGGAG TCTGAGCAGA TGGGACCTGT GGAGACTGAG 
GCTGAGCAAG GCGGCCTGCT GCCTGCACCC TCGGTGTCCC CACAGGAGCC CTCCAAGGAG 
TTCGATGAGG ACTCCTTGAT TCCCAGCAGC CCGGCCACCG AAACCTCGGA CAACATCAGC 
CCCGTGGCCA GCCCCGTTCA CACAGGGTTC CTGGTGAGCT TCATGGTGGA TGCGCGTGGC 
GGCTCCATGC GGGGCAGCCG GCACCACGGG CTGCGCGTGG TCATCCCTCC CCGTGCCTGT 
GCTGCACCCA CCCGCATCAC TTGCCGCCTG GTGAAGCCTC AGAAGCTGCC TGCCCCCCCA 
CCGCTGGCTG AGGAGGAGGG CCTGGCAAGC CGCATCATTG CACTGGGGCC CGCTGGCGCC 
CAGTTCCTCA GCCCCGTCAT TGTGGAGATT CCTCACTTTG CCTCCTATGG ACGTGGGGAC 
CGTGAGCTGG TGGTGTTGCG CAGTGAGAAT GGCTCTGTCT GGAAGGAGCA CCGCAACCGC 
TACGAGGAGA GCTACATGGA CCAACTGCTC AGTGGCATGG ATGAGGAGCT GGAGAGCCAA 
GAGGAGCTGG AGAAGAAGAG GGTCTGCCGC ATCATCACCA CTGACTTTCC TCTCTACTTC 
GTGGTCATGT CCCGGATTTG CCAGGACTGT GACATGATTG GCCCCGAGGG AGGGTGCTTG 
AAAAGCACAC TGGTGCCCAT GGTGCAGGCC ACCTTCCCAG ACACCGCTGT CACCAAGAAA 
GTGAGGCTGG CTCTGCAGGC GCAGCCCGTG CCTGATGAGC TGGTGACCAA GCTGCTGGGG 
AACCAGGCAA CCTTCAGCCC CATCGTCACA GTGGAGCCAC GTCGGAGGAA GTTCCACCGC 
CCCATTGGCC TCCGCATCCC TCTGCCCCCA TCCTGGAAGG ACAATCCCCG GGACAGCGGC 
GAGGGTGACA CCACCAGCCT GCGCCTGCTC TGTTCTGTGA TAGGAGGGAC AGCACAAGCT 
CAGTGGGAAG ACATCACAGG CACCACAAAG CTGGTCTATG AAAAAGAGTG TGCTAACTTT 
ACCACCAATG TGTCTGCCAG GTTCTGGCTG GCTGACTGCC CGCGCACTGC TGAGGCTGTG 
CACTTTGCCA CCGTGCTGTA CAAGGAACTG ACGGCTGTGC CCTACATGGC CAAATTTGTG 
GTGTTTGCTA AGATGAACGA TGGACGGGAA GGCCGGCTGC GCTGCTACTG CATGACTGAT 
GACAAGGTGG ACAAGACTTT GGAGCATCAT GAGAACTTCA CTGAGGTGGC CCGCAGCAGG 
GACATTGAGG TGGTGGAAGG AATGCCTCTG CATGTCGAGC TCTCAGGGAA CCTGGTGCCC 
GTCAAGAAGG CCACGCAGTC ACGCACCTTC TTCTTCCAGT CCTTCCGGGA AAGCCGCCTC 
GCCATCCCCA TCAAGGTCCG GGACAGCAGC CGGGAAGCCA GTGGCTCCCT GTCCTTCCTG 
CGCAAGGCCA TGAAGTATGA GGACTTGCAG CATGTGCTCT GCCACCTCAA CATCAGCATT 
CCACCCTGCA CCAAGGGAAG CAGCACTGAG GAGCGAAGGA GGACACTGAC ACCTTTGTCT 
CTGCGCTCTC TGAGCAGCAC CGACAGGGCA GACCAGAAGA TGATCGACAT TGCAGAGCAG 
CTTGGCCTCA GCTGGGCAGA GCTGGCACGT GAGCTGCAGT TTGGGGTGGA TGACATCAAC 
AGGATTCGTG TGGAGAACCC TAACTCCTTG CTGGAGCAGA GCATAGCCTT GCTTAACCTC 
TGGGTCAGCC GCGAGGGCAA GGGTGTCAAA ATTGAGAACC TGTACACAGC GCTGAGGAAC 
ATTGACCGCA GCGAGATCAT CAGCACGTTG GAGGGCTCCG GCCGGCAGAG CCGCAGCATG 
AAGGGCAGCT GGCGTTACAT GGACAGGGAC TATTCACTCT CGCCCTCCCA GATGAATGGT 
TACGCTTCGC TGCAGGACGA GCTGCTCTCC CCTGCCTCCC TGCATTATAT GCTGCCATCC 
CCTCTGCGTG CTGACCAGTA CTGGAACGAG GTGGCCATCA TGGACGCCAT CCCCATGGCC 
GCCACTGAGC AGGACGCCCT GATGGAGATG TCTGACATGC AGGTGTGGTC CTCAGGGCTC 
ACCCCTTCAC TGGTGACGGC TGAGGACTCC TCTCTGGAGT GCAGCAAGGC TGAGGACTCG 
GATGCTACAA GCGAAGGTCG TTTCCCAGGG CACCTTCTGA CAGATGCACA TGTCCCAGAC 
CACATGGGCT CTATGGACTT AGTTGAGGAT GACACAGTGG ACTCAGACGC CATGAACGGG 
CTGATTGAAC TGCTAGAGCA GGAGGAGGGG CAGAGGCCAG AGGGGAAGAT GCCAGCCAGC 
GACCACCAGC TAGGGACTGG AGAGCAGAAC CCAGAGAGTG AAGTCTCTTT TGTTTCAGTT 
CAACAGAAGG TACAAGCCAC GATCGTGCCA TCGCCAACCG TGAGCCACAC AGTGGAGAGG 
AGTGCAGACA GGGCAACGAA GTCCTCCATC TCCCTGGGGA GCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152884.1
CDS	887..6274
Translation	MAQAAKQLKKIKDIEAQALQEQKEKEESNRKRRNRSRDRKKKRGHAPTTVDPLPSCCKQGSPEGMLEQESAVQADAATSFLRAARSGNLDRALDHLRNGVDINTCNQNGLNALHLASKEGHTKMVVELLHKEIVLETTTKKGNTALHIAALAGQQDVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLSVAQLLLNRGASVNFTPQNGITPLHIASRRGNIIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRIAEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYSAEIDDITLDHLTPLHVAAHCGHHRVAKLLVEKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVAAFMGHLPIVKTLLQRGASPNVSNVKVETPLHMAARAGHMDVAKYLIQNKAKINAKAKDDQTPLHCAARIGHTSMVQLLLENNADPNLATTAGHTPLHIAAREGHVDTALALLEKGASQTCMTKKGFTPLHVAAKYGKVDVAELLLVHDAHPNAAGKNGLTPLHVAVHHNNLEIVKLLLPKGSSPHSSAWNGYTPLHIAAKQNQMEVASSLLQYGASANAESVQGVTPLHLASQEGHTDMVALLFSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLVKHGVTVDATTRMGYTSLHIASHYGNIKLVKFLLQHQADVNAKTKLGYTPLHQAAQQGHTDVVTLLLKHGASPNEISTNGTTPLAIAKRLGYISVTDVLKIVTEETTIPPFSDKYRMSFPETVDEILDVSEDEGTAHVTVMEEELVPPKPRTPEPKEQEGKREMLEFVTVTTLEQMEESAAVPQVPCVPPETVVTIAEESEQMGPVETEAEQGGLLPAPSVSPQEPSKEFDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHHGLRVVIPPRACAAPTRITCRLVKPQKLPAPPPLAEEEGLASRIIALGPAGAQFLSPVIVEIPHFASYGRGDRELVVLRSENGSVWKEHRNRYEESYMDQLLSGMDEELESQEELEKKRVCRIITTDFPLYFVVMSRICQDCDMIGPEGGCLKSTLVPMVQATFPDTAVTKKVRLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWKDNPRDSGEGDTTSLRLLCSVIGGTAQAQWEDITGTTKLVYEKECANFTTNVSARFWLADCPRTAEAVHFATVLYKELTAVPYMAKFVVFAKMNDGREGRLRCYCMTDDKVDKTLEHHENFTEVARSRDIEVVEGMPLHVELSGNLVPVKKATQSRTFFFQSFRESRLAIPIKVRDSSREASGSLSFLRKAMKYEDLQHVLCHLNISIPPCTKGSSTEERRRTLTPLSLRSLSSTDRADQKMIDIAEQLGLSWAELARELQFGVDDINRIRVENPNSLLEQSIALLNLWVSREGKGVKIENLYTALRNIDRSEIISTLEGSGRQSRSMKGSWRYMDRDYSLSPSQMNGYASLQDELLSPASLHYMLPSPLRADQYWNEVAIMDAIPMAATEQDALMEMSDMQVWSSGLTPSLVTAEDSSLECSKAEDSDATSEGRFPGHLLTDAHVPDHMGSMDLVEDDTVDSDAMNGLIELLEQEEGQRPEGKMPASDHQLGTGEQNPESEVSFVSVQQKVQATIVPSPTVSHTVERSADRATKSSISLGSM

Target ORF information:

RefSeq Version	XM_015297398.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297398.1

ORF Insert Sequence:

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGAGAG GGCATGCACC AACCACTGTG GACCCCTTGC CTTCTTGTTG CAAGCAAGGA 
TCTCCTGAAG GGATGCTGGA GCAGGAGTCT GCTGTGCAGG CTGATGCTGC GACCAGCTTC 
CTGAGAGCTG CAAGATCTGG GAATCTGGAC AGAGCCTTGG ATCATCTCAG GAATGGGGTA 
GATATTAACA CCTGTAACCA GAATGGGCTG AATGCCTTGC ACCTGGCCTC CAAGGAGGGC 
CACACGAAAA TGGTGGTGGA GCTTCTGCAC AAGGAGATTG TTTTGGAGAC CACTACCAAG 
AAGGGAAACA CAGCCCTGCA CATCGCTGCC CTCGCTGGAC AGCAGGACGT GGTCCGGGAA 
CTGGTGAACT ATGGGGCCAA TGTCAATGCT CAGTCGCAGA AGGGCTTCAC ACCACTCTAC 
ATGGCAGCGC AGGAAAACCA CCTGGAAGTT GTCAAATTCC TGCTGGAAAA CGGAGCCAAC 
CAGAATGTAG CCACAGAGGA TGGCTTCACA CCACTAGCTG TGGCTCTGCA GCAGGGTCAT 
GAGAATGTGG TTGCTCACCT TATCAACTAT GGGACGAAGG GTAAGGTCCG CCTGCCTGCC 
TTGCACATTG CAGCTCGCAA CGATGACACC CGTACAGCCG CCGTGCTGCT GCAGAATGAC 
CCCAATGCTG ACGTCCTCTC CAAGACTGGA TTTACACCTT TGCACATTGC AGCCCACTAT 
GAGAATCTCA GCGTGGCCCA ATTATTGCTG AATCGTGGAG CCAGCGTCAA CTTCACACCC 
CAGAATGGGA TCACTCCTTT GCACATAGCC TCTCGTCGAG GCAACATCAT CATGGTACGG 
CTGCTGCTGG ACCGTGGGGC CCAGATAGAG ACAAGGACCA AGGATGAGTT GACGCCTCTC 
CACTGTGCAG CTCGCAATGG ACATGTGCGA ATTGCAGAGA TCCTACTGGA CCATGGTGCT 
CCCATTCAAG CCAAAACAAA GAACGGTCTG TCACCGATCC ACATGGCAGC ACAGGGTGAC 
CACCTGGACT GTGTGCGCCT ACTCCTGCAG TACAGTGCTG AGATAGACGA CATCACCCTG 
GACCACCTGA CACCACTGCA TGTAGCTGCG CACTGTGGCC ACCACCGTGT GGCCAAGCTG 
CTGGTGGAGA AGGGAGCCAA GCCCAACTCC AGAGCTCTGA ATGGCTTCAC GCCTCTCCAC 
ATTGCCTGCA AGAAGAACCA CATTCGGGTG ATGGAGTTGC TTCTGAAGAC AGGAGCCTCC 
ATTGATGCTG TCACAGAGTC TGGCCTGACC CCCTTGCATG TGGCTGCCTT CATGGGGCAC 
TTGCCCATTG TCAAAACCTT GCTGCAGCGT GGAGCATCTC CTAATGTGTC CAATGTGAAA 
GTGGAGACTC CCCTGCACAT GGCAGCCAGA GCTGGGCACA TGGATGTGGC CAAGTATCTG 
ATACAGAACA AGGCCAAAAT CAACGCCAAG GCCAAGGATG ACCAGACTCC TCTGCACTGT 
GCTGCACGCA TTGGCCACAC CAGCATGGTC CAACTCCTGC TGGAGAACAA TGCCGACCCC 
AACCTGGCCA CTACAGCAGG GCACACGCCT CTGCACATCG CTGCCAGAGA GGGGCATGTA 
GACACAGCCT TGGCCCTGCT AGAGAAGGGA GCCTCTCAGA CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TCCACGTTGC AGCCAAGTAT GGGAAGGTGG ATGTGGCAGA GCTGCTGCTG 
GTGCATGATG CTCACCCCAA TGCAGCGGGG AAGAACGGCC TGACCCCACT GCACGTAGCT 
GTGCACCACA ACAACCTGGA GATCGTCAAG CTTCTGCTTC CCAAGGGGAG CTCTCCACAC 
AGCTCAGCCT GGAACGGGTA CACCCCTCTG CACATTGCCG CCAAGCAGAA CCAGATGGAG 
GTGGCCAGCA GCTTGCTGCA GTATGGTGCT TCTGCAAATG CGGAGTCTGT TCAGGGAGTC 
ACCCCTCTGC ACCTGGCTTC TCAGGAAGGG CATACAGACA TGGTAGCACT GCTTTTCTCC 
AAGCAAGCCA ATGGAAACTT AGGCAACAAG AGTGGTCTGA CTCCTCTCCA TCTCGTGGCC 
CAAGAGGGAC ACGTACCAGT GGCTGATGTT CTGGTGAAAC ATGGAGTCAC AGTGGATGCG 
ACAACCAGGA TGGGCTACAC CTCACTGCAC ATAGCCAGCC ACTATGGGAA CATCAAGTTG 
GTGAAGTTTC TGCTGCAGCA CCAGGCTGAT GTCAATGCTA AGACTAAGCT GGGCTACACA 
CCCCTGCACC AGGCGGCACA GCAGGGCCAC ACGGATGTGG TGACCCTGCT GCTGAAGCAT 
GGTGCCTCTC CCAACGAGAT CAGCACAAAT GGCACCACTC CCCTGGCCAT TGCGAAGCGC 
CTCGGTTACA TTTCCGTCAC AGATGTGCTC AAGATAGTCA CAGAGGAGAC CACCATCCCA 
CCATTCAGTG ACAAGTACCG CATGAGCTTC CCGGAGACCG TGGATGAGAT TCTGGATGTG 
TCGGAGGATG AAGGCACTGC TCATGTCACA GTAATGGAGG AGGAGCTGGT CCCACCGAAA 
CCCAGAACAC CTGAACCCAA GGAGCAGGAG GGCAAGAGGG AGATGCTGGA GTTTGTGACC 
GTGACAACAC TGGAGCAAAT GGAGGAATCT GCAGCTGTCC CACAGGTCCC CTGCGTGCCC 
CCTGAGACTG TGGTGACCAT AGCAGAGGAG TCTGAGCAGA TGGGACCTGT GGAGACTGAG 
GCTGAGCAAG GCGGCCTGCT GCCTGCACCC TCGGTGTCCC CACAGGAGCC CTCCAAGGAG 
TTCGATGAGG ACTCCTTGAT TCCCAGCAGC CCGGCCACCG AAACCTCGGA CAACATCAGC 
CCCGTGGCCA GCCCCGTTCA CACAGGGTTC CTGGTGAGCT TCATGGTGGA TGCGCGTGGC 
GGCTCCATGC GGGGCAGCCG GCACCACGGG CTGCGCGTGG TCATCCCTCC CCGTGCCTGT 
GCTGCACCCA CCCGCATCAC TTGCCGCCTG GTGAAGCCTC AGAAGCTGCC TGCCCCCCCA 
CCGCTGGCTG AGGAGGAGGG CCTGGCAAGC CGCATCATTG CACTGGGGCC CGCTGGCGCC 
CAGTTCCTCA GCCCCGTCAT TGTGGAGATT CCTCACTTTG CCTCCTATGG ACGTGGGGAC 
CGTGAGCTGG TGGTGTTGCG CAGTGAGAAT GGCTCTGTCT GGAAGGAGCA CCGCAACCGC 
TACGAGGAGA GCTACATGGA CCAACTGCTC AGTGGCATGG ATGAGGAGCT GGAGAGCCAA 
GAGGAGCTGG AGAAGAAGAG GGTCTGCCGC ATCATCACCA CTGACTTTCC TCTCTACTTC 
GTGGTCATGT CCCGGATTTG CCAGGACTGT GACATGATTG GCCCCGAGGG AGGGTGCTTG 
AAAAGCACAC TGGTGCCCAT GGTGCAGGCC ACCTTCCCAG ACACCGCTGT CACCAAGAAA 
GTGAGGCTGG CTCTGCAGGC GCAGCCCGTG CCTGATGAGC TGGTGACCAA GCTGCTGGGG 
AACCAGGCAA CCTTCAGCCC CATCGTCACA GTGGAGCCAC GTCGGAGGAA GTTCCACCGC 
CCCATTGGCC TCCGCATCCC TCTGCCCCCA TCCTGGAAGG ACAATCCCCG GGACAGCGGC 
GAGGGTGACA CCACCAGCCT GCGCCTGCTC TGTTCTGTGA TAGGAGGGAC AGCACAAGCT 
CAGTGGGAAG ACATCACAGG CACCACAAAG CTGGTCTATG AAAAAGAGTG TGCTAACTTT 
ACCACCAATG TGTCTGCCAG GTTCTGGCTG GCTGACTGCC CGCGCACTGC TGAGGCTGTG 
CACTTTGCCA CCGTGCTGTA CAAGGAACTG ACGGCTGTGC CCTACATGGC CAAATTTGTG 
GTGTTTGCTA AGATGAACGA TGGACGGGAA GGCCGGCTGC GCTGCTACTG CATGACTGAT 
GACAAGGTGG ACAAGACTTT GGAGCATCAT GAGAACTTCA CTGAGGTGGC CCGCAGCAGG 
GACATTGAGG TGGTGGAAGG AATGCCTCTG CATGTCGAGC TCTCAGGGAA CCTGGTGCCC 
GTCAAGAAGG CCACGCAGTC ACGCACCTTC TTCTTCCAGT CCTTCCGGGA AAGCCGCCTC 
GCCATCCCCA TCAAGGTCCG GGACAGCAGC CGGGAAGCCA GTGGCTCCCT GTCCTTCCTG 
CGCAAGGCCA TGAAGTATGA GGACTTGCAG CATGTGCTCT GCCACCTCAA CATCAGCATT 
CCACCCTGCA CCAAGGGAAG CAGCACTGAG GAGCGAAGGA GGACACTGAC ACCTTTGTCT 
CTGCGCTCTC TGAGCAGCAC CGACAGGGCA GACCAGAAGA TGATCGACAT TGCAGAGCAG 
CTTGGCCTCA GCTGGGCAGA GCTGGCACGT GAGCTGCAGT TTGGGGTGGA TGACATCAAC 
AGGATTCGTG TGGAGAACCC TAACTCCTTG CTGGAGCAGA GCATAGCCTT GCTTAACCTC 
TGGGTCAGCC GCGAGGGCAA GGGTGTCAAA ATTGAGAACC TGTACACAGC GCTGAGGAAC 
ATTGACCGCA GCGAGATCAT CAGCACGTTG GAGGGCTCCG GCCGGCAGAG CCGCAGCATG 
AAGGGCAGCT GGCGTTACAT GGACAGGGAC TATTCACTCT CGCCCTCCCA GATGAATGGT 
TACGCTTCGC TGCAGGACGA GCTGCTCTCC CCTGCCTCCC TGCATTATAT GCTGCCATCC 
CCTCTGCGTG CTGACCAGTA CTGGAACGAG GTGGCCATCA TGGACGCCAT CCCCATGGCC 
GCCACTGAGC AGGACGCCCT GATGGAGATG TCTGACATGC AGGTGTGGTC CTCAGGGCTC 
ACCCCTTCAC TGGTGACGGC TGAGGACTCC TCTCTGGAGT GCAGCAAGGC TGAGGACTCG 
GATGCTACAA GCGAAGGTCG TTTCCCAGGG CACCTTCTGA CAGATGCACA TGTCCCAGAC 
CACATGGGCT CTATGGACTT AGTTGAGGAT GACACAGTGG ACTCAGACGC CATGAACGGG 
CTGATTGAAC TGCTAGAGCA GGAGGAGGGG CAGAGGCCAG AGGGGAAGAT GCCAGCCAGC 
GACCACCAGC TAGGGACTGG AGAGCAGAAC CCAGAGAGTG AAGTCTCTTT TGTTTCAGTT 
CAACAGAAGG TACAAGCCAC GATCGTGCCA TCGCCAACCG TGAGCCACAC AGTGGAGAGG 
AGTGCAGACA GGGCAACGAA GTCCTCCATC TCCCTGGGGA GCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK1 ( XM_015297399.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152885.1 Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X10, mRNA.

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CloneID	OGa45394
Clone ID Related Accession (Same CDS sequence)	XM_015297399.1
Accession Version	XM_015297399.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ankyrin-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455999.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGAGAG GGCATGCACC AACCACTGTG GACCCCTTGC CTTCTTGTTG CAAGCAAGGA 
TCTCCTGAAG GGATGCTGGA GCAGGAGTCT GCTGTGCAGG CTGATGCTGC GACCAGCTTC 
CTGAGAGCTG CAAGATCTGG GAATCTGGAC AGAGCCTTGG ATCATCTCAG GAATGGGGTA 
GATATTAACA CCTGTAACCA GAATGGGCTG AATGCCTTGC ACCTGGCCTC CAAGGAGGGC 
CACACGAAAA TGGTGGTGGA GCTTCTGCAC AAGGAGATTG TTTTGGAGAC CACTACCAAG 
AAGGGAAACA CAGCCCTGCA CATCGCTGCC CTCGCTGGAC AGCAGGACGT GGTCCGGGAA 
CTGGTGAACT ATGGGGCCAA TGTCAATGCT CAGTCGCAGA AGGGCTTCAC ACCACTCTAC 
ATGGCAGCGC AGGAAAACCA CCTGGAAGTT GTCAAATTCC TGCTGGAAAA CGGAGCCAAC 
CAGAATGTAG CCACAGAGGA TGGCTTCACA CCACTAGCTG TGGCTCTGCA GCAGGGTCAT 
GAGAATGTGG TTGCTCACCT TATCAACTAT GGGACGAAGG GTAAGGTCCG CCTGCCTGCC 
TTGCACATTG CAGCTCGCAA CGATGACACC CGTACAGCCG CCGTGCTGCT GCAGAATGAC 
CCCAATGCTG ACGTCCTCTC CAAGACTGGA TTTACACCTT TGCACATTGC AGCCCACTAT 
GAGAATCTCA GCGTGGCCCA ATTATTGCTG AATCGTGGAG CCAGCGTCAA CTTCACACCC 
CAGAATGGGA TCACTCCTTT GCACATAGCC TCTCGTCGAG GCAACATCAT CATGGTACGG 
CTGCTGCTGG ACCGTGGGGC CCAGATAGAG ACAAGGACCA AGGATGAGTT GACGCCTCTC 
CACTGTGCAG CTCGCAATGG ACATGTGCGA ATTGCAGAGA TCCTACTGGA CCATGGTGCT 
CCCATTCAAG CCAAAACAAA GAACGGTCTG TCACCGATCC ACATGGCAGC ACAGGGTGAC 
CACCTGGACT GTGTGCGCCT ACTCCTGCAG TACAGTGCTG AGATAGACGA CATCACCCTG 
GACCACCTGA CACCACTGCA TGTAGCTGCG CACTGTGGCC ACCACCGTGT GGCCAAGCTG 
CTGGTGGAGA AGGGAGCCAA GCCCAACTCC AGAGCTCTGA ATGGCTTCAC GCCTCTCCAC 
ATTGCCTGCA AGAAGAACCA CATTCGGGTG ATGGAGTTGC TTCTGAAGAC AGGAGCCTCC 
ATTGATGCTG TCACAGAGTC TGGCCTGACC CCCTTGCATG TGGCTGCCTT CATGGGGCAC 
TTGCCCATTG TCAAAACCTT GCTGCAGCGT GGAGCATCTC CTAATGTGTC CAATGTGAAA 
GTGGAGACTC CCCTGCACAT GGCAGCCAGA GCTGGGCACA TGGATGTGGC CAAGTATCTG 
ATACAGAACA AGGCCAAAAT CAACGCCAAG GCCAAGGATG ACCAGACTCC TCTGCACTGT 
GCTGCACGCA TTGGCCACAC CAGCATGGTC CAACTCCTGC TGGAGAACAA TGCCGACCCC 
AACCTGGCCA CTACAGCAGG GCACACGCCT CTGCACATCG CTGCCAGAGA GGGGCATGTA 
GACACAGCCT TGGCCCTGCT AGAGAAGGGA GCCTCTCAGA CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TCCACGTTGC AGCCAAGTAT GGGAAGGTGG ATGTGGCAGA GCTGCTGCTG 
GTGCATGATG CTCACCCCAA TGCAGCGGGG AAGAACGGCC TGACCCCACT GCACGTAGCT 
GTGCACCACA ACAACCTGGA GATCGTCAAG CTTCTGCTTC CCAAGGGGAG CTCTCCACAC 
AGCTCAGCCT GGAACGGGTA CACCCCTCTG CACATTGCCG CCAAGCAGAA CCAGATGGAG 
GTGGCCAGCA GCTTGCTGCA GTATGGTGCT TCTGCAAATG CGGAGTCTGT TCAGGGAGTC 
ACCCCTCTGC ACCTGGCTTC TCAGGAAGGG CATACAGACA TGGTAGCACT GCTTTTCTCC 
AAGCAAGCCA ATGGAAACTT AGGCAACAAG AGTGGTCTGA CTCCTCTCCA TCTCGTGGCC 
CAAGAGGGAC ACGTACCAGT GGCTGATGTT CTGGTGAAAC ATGGAGTCAC AGTGGATGCG 
ACAACCAGGA TGGGCTACAC CTCACTGCAC ATAGCCAGCC ACTATGGGAA CATCAAGTTG 
GTGAAGTTTC TGCTGCAGCA CCAGGCTGAT GTCAATGCTA AGACTAAGCT GGGCTACACA 
CCCCTGCACC AGGCGGCACA GCAGGGCCAC ACGGATGTGG TGACCCTGCT GCTGAAGCAT 
GGTGCCTCTC CCAACGAGAT CAGCACAAAT GGCACCACTC CCCTGGCCAT TGCGAAGCGC 
CTCGGTTACA TTTCCGTCAC AGATGTGCTC AAGATAGTCA CAGAGGAGAC CACCATCCCA 
CCATTCAGTG ACAAGTACCG CATGAGCTTC CCGGAGACCG TGGATGAGAT TCTGGATGTG 
TCGGAGGATG AAGGCACTGC TCATGTCACA GTAATGGAGG AGGAGCTGGT CCCACCGAAA 
CCCAGAACAC CTGAACCCAA GGAGCAGGAG GGCAAGAGGG AGATGCTGGA GTTTGTGACC 
GTGACAACAC TGGAGCAAAT GGAGGAATCT GCAGCTGTCC CACAGGTCCC CTGCGTGCCC 
CCTGAGACTG TGGTGACCAT AGCAGAGGAG TCTGAGCAGA TGGGACCTGT GGAGACTGAG 
GCTGAGCAAG GCGGCCTGCT GCCTGCACCC TCGGTGTCCC CACAGGAGCC CTCCAAGGAG 
TTCGATGAGG ACTCCTTGAT TCCCAGCAGC CCGGCCACCG AAACCTCGGA CAACATCAGC 
CCCGTGGCCA GCCCCGTTCA CACAGGGTTC CTGGTGAGCT TCATGGTGGA TGCGCGTGGC 
GGCTCCATGC GGGGCAGCCG GCACCACGGG CTGCGCGTGG TCATCCCTCC CCGTGCCTGT 
GCTGCACCCA CCCGCATCAC TTGCCGCCTG GTGAAGCCTC AGAAGCTGCC TGCCCCCCCA 
CCGCTGGCTG AGGAGGAGGG CCTGGCAAGC CGCATCATTG CACTGGGGCC CGCTGGCGCC 
CAGTTCCTCA GCCCCGTCAT TGTGGAGATT CCTCACTTTG CCTCCTATGG ACGTGGGGAC 
CGTGAGCTGG TGGTGTTGCG CAGTGAGAAT GGCTCTGTCT GGAAGGAGCA CCGCAACCGC 
TACGAGGAGA GCTACATGGA CCAACTGCTC AGTGGCATGG ATGAGGAGCT GGAGAGCCAA 
GAGGAGCTGG AGAAGAAGAG GGTCTGCCGC ATCATCACCA CTGACTTTCC TCTCTACTTC 
GTGGTCATGT CCCGGATTTG CCAGGACTGT GACATGATTG GCCCCGAGGG AGGGTGCTTG 
AAAAGCACAC TGGTGCCCAT GGTGCAGGCC ACCTTCCCAG ACACCGCTGT CACCAAGAAA 
GTGAGGCTGG CTCTGCAGGC GCAGCCCGTG CCTGATGAGC TGGTGACCAA GCTGCTGGGG 
AACCAGGCAA CCTTCAGCCC CATCGTCACA GTGGAGCCAC GTCGGAGGAA GTTCCACCGC 
CCCATTGGCC TCCGCATCCC TCTGCCCCCA TCCTGGAAGG ACAATCCCCG GGACAGCGGC 
GAGGGTGACA CCACCAGCCT GCGCCTGCTC TGTTCTGTGA TAGGAGGGAC AGCACAAGCT 
CAGTGGGAAG ACATCACAGG CACCACAAAG CTGGTCTATG AAAAAGAGTG TGCTAACTTT 
ACCACCAATG TGTCTGCCAG GTTCTGGCTG GCTGACTGCC CGCGCACTGC TGAGGCTGTG 
CACTTTGCCA CCGTGCTGTA CAAGGAACTG ACGGCTGTGC CCTACATGGC CAAATTTGTG 
GTGTTTGCTA AGATGAACGA TGGACGGGAA GGCCGGCTGC GCTGCTACTG CATGACTGAT 
GACAAGGTGG ACAAGACTTT GGAGCATCAT GAGAACTTCA CTGAGGTGGC CCGCAGCAGG 
GACATTGAGG TGGTGGAAGG AATGCCTCTG CATGTCGAGC TCTCAGGGAA CCTGGTGCCC 
GTCAAGAAGG CCACGCAGTC ACGCACCTTC TTCTTCCAGT CCTTCCGGGA AAGCCGCCTC 
GCCATCCCCA TCAAGGTCCG GGACAGCAGC CGGGAAGCCA GTGGCTCCCT GTCCTTCCTG 
CGCAAGGCCA TGAAGTATGA GGACTTGCAG CATGTGCTCT GCCACCTCAA CATCAGCATT 
CCACCCTGCA CCAAGGGAAG CAGCACTGAG GAGCGAAGGA GGACACTGAC ACCTTTGTCT 
CTGCGCTCTC TGAGCAGCAC CGACAGGGCA GACCAGAAGA TGATCGACAT TGCAGAGCAG 
CTTGGCCTCA GCTGGGCAGA GCTGGCACGT GAGCTGCAGT TTGGGGTGGA TGACATCAAC 
AGGATTCGTG TGGAGAACCC TAACTCCTTG CTGGAGCAGA GCATAGCCTT GCTTAACCTC 
TGGGTCAGCC GCGAGGGCAA GGGTGTCAAA ATTGAGAACC TGTACACAGC GCTGAGGAAC 
ATTGACCGCA GCGAGATCAT CAGCACGTTG GAGGGCTCCG GCCGGCAGAG CCGCAGCATG 
AAGGGCAGCT GGCGTTACAT GGACAGGGAC TATTCACTCT CGCCCTCCCA GATGAATGGT 
TACGCTTCGC TGCAGGACGA GCTGCTCTCC CCTGCCTCCC TGCATTATAT GCTGCCATCC 
CCTCTGCGTG CTGACCAGTA CTGGAACGAG GTGGCCATCA TGGACGCCAT CCCCATGGCC 
GCCACTGAGC AGGACGCCCT GATGGAGATG TCTGACATGC AGGTGTGGTC CTCAGGGCTC 
ACCCCTTCAC TGGTGACGGC TGAGGACTCC TCTCTGGAGT GCAGCAAGGC TGAGGACTCG 
GATGCTACAA GCGAAGGTCG TTTCCCAGGG CACCTTCTGA CAGATGCACA TGTCCCAGAC 
CACATGGGCT CTATGGACTT AGTTGAGGAT GACACAGTGG ACTCAGACGC CATGAACGGG 
CTGATTGAAC TGCTAGAGCA GGAGGAGGGG CAGAGGCCAG AGGGGAAGAT GCCAGCCAGC 
GACCACCAGC TAGGGACTGG AGAGCAGAAC CCAGAGAGTG AAGTCTCTTT TGTTTCAGTT 
CAACAGAAGG TACAAGCCAC GATCGTGCCA TCGCCAACCG TGAGCCACAC AGTGGAGAGG 
AGTGCAGACA GGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152885.1
CDS	888..6245
Translation	MAQAAKQLKKIKDIEAQALQEQKEKEESNRKRRNRSRDRKKKRGHAPTTVDPLPSCCKQGSPEGMLEQESAVQADAATSFLRAARSGNLDRALDHLRNGVDINTCNQNGLNALHLASKEGHTKMVVELLHKEIVLETTTKKGNTALHIAALAGQQDVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLSVAQLLLNRGASVNFTPQNGITPLHIASRRGNIIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRIAEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYSAEIDDITLDHLTPLHVAAHCGHHRVAKLLVEKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVAAFMGHLPIVKTLLQRGASPNVSNVKVETPLHMAARAGHMDVAKYLIQNKAKINAKAKDDQTPLHCAARIGHTSMVQLLLENNADPNLATTAGHTPLHIAAREGHVDTALALLEKGASQTCMTKKGFTPLHVAAKYGKVDVAELLLVHDAHPNAAGKNGLTPLHVAVHHNNLEIVKLLLPKGSSPHSSAWNGYTPLHIAAKQNQMEVASSLLQYGASANAESVQGVTPLHLASQEGHTDMVALLFSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLVKHGVTVDATTRMGYTSLHIASHYGNIKLVKFLLQHQADVNAKTKLGYTPLHQAAQQGHTDVVTLLLKHGASPNEISTNGTTPLAIAKRLGYISVTDVLKIVTEETTIPPFSDKYRMSFPETVDEILDVSEDEGTAHVTVMEEELVPPKPRTPEPKEQEGKREMLEFVTVTTLEQMEESAAVPQVPCVPPETVVTIAEESEQMGPVETEAEQGGLLPAPSVSPQEPSKEFDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHHGLRVVIPPRACAAPTRITCRLVKPQKLPAPPPLAEEEGLASRIIALGPAGAQFLSPVIVEIPHFASYGRGDRELVVLRSENGSVWKEHRNRYEESYMDQLLSGMDEELESQEELEKKRVCRIITTDFPLYFVVMSRICQDCDMIGPEGGCLKSTLVPMVQATFPDTAVTKKVRLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWKDNPRDSGEGDTTSLRLLCSVIGGTAQAQWEDITGTTKLVYEKECANFTTNVSARFWLADCPRTAEAVHFATVLYKELTAVPYMAKFVVFAKMNDGREGRLRCYCMTDDKVDKTLEHHENFTEVARSRDIEVVEGMPLHVELSGNLVPVKKATQSRTFFFQSFRESRLAIPIKVRDSSREASGSLSFLRKAMKYEDLQHVLCHLNISIPPCTKGSSTEERRRTLTPLSLRSLSSTDRADQKMIDIAEQLGLSWAELARELQFGVDDINRIRVENPNSLLEQSIALLNLWVSREGKGVKIENLYTALRNIDRSEIISTLEGSGRQSRSMKGSWRYMDRDYSLSPSQMNGYASLQDELLSPASLHYMLPSPLRADQYWNEVAIMDAIPMAATEQDALMEMSDMQVWSSGLTPSLVTAEDSSLECSKAEDSDATSEGRFPGHLLTDAHVPDHMGSMDLVEDDTVDSDAMNGLIELLEQEEGQRPEGKMPASDHQLGTGEQNPESEVSFVSVQQKVQATIVPSPTVSHTVERSADRT

Target ORF information:

RefSeq Version	XM_015297399.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297399.1

ORF Insert Sequence:

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGAGAG GGCATGCACC AACCACTGTG GACCCCTTGC CTTCTTGTTG CAAGCAAGGA 
TCTCCTGAAG GGATGCTGGA GCAGGAGTCT GCTGTGCAGG CTGATGCTGC GACCAGCTTC 
CTGAGAGCTG CAAGATCTGG GAATCTGGAC AGAGCCTTGG ATCATCTCAG GAATGGGGTA 
GATATTAACA CCTGTAACCA GAATGGGCTG AATGCCTTGC ACCTGGCCTC CAAGGAGGGC 
CACACGAAAA TGGTGGTGGA GCTTCTGCAC AAGGAGATTG TTTTGGAGAC CACTACCAAG 
AAGGGAAACA CAGCCCTGCA CATCGCTGCC CTCGCTGGAC AGCAGGACGT GGTCCGGGAA 
CTGGTGAACT ATGGGGCCAA TGTCAATGCT CAGTCGCAGA AGGGCTTCAC ACCACTCTAC 
ATGGCAGCGC AGGAAAACCA CCTGGAAGTT GTCAAATTCC TGCTGGAAAA CGGAGCCAAC 
CAGAATGTAG CCACAGAGGA TGGCTTCACA CCACTAGCTG TGGCTCTGCA GCAGGGTCAT 
GAGAATGTGG TTGCTCACCT TATCAACTAT GGGACGAAGG GTAAGGTCCG CCTGCCTGCC 
TTGCACATTG CAGCTCGCAA CGATGACACC CGTACAGCCG CCGTGCTGCT GCAGAATGAC 
CCCAATGCTG ACGTCCTCTC CAAGACTGGA TTTACACCTT TGCACATTGC AGCCCACTAT 
GAGAATCTCA GCGTGGCCCA ATTATTGCTG AATCGTGGAG CCAGCGTCAA CTTCACACCC 
CAGAATGGGA TCACTCCTTT GCACATAGCC TCTCGTCGAG GCAACATCAT CATGGTACGG 
CTGCTGCTGG ACCGTGGGGC CCAGATAGAG ACAAGGACCA AGGATGAGTT GACGCCTCTC 
CACTGTGCAG CTCGCAATGG ACATGTGCGA ATTGCAGAGA TCCTACTGGA CCATGGTGCT 
CCCATTCAAG CCAAAACAAA GAACGGTCTG TCACCGATCC ACATGGCAGC ACAGGGTGAC 
CACCTGGACT GTGTGCGCCT ACTCCTGCAG TACAGTGCTG AGATAGACGA CATCACCCTG 
GACCACCTGA CACCACTGCA TGTAGCTGCG CACTGTGGCC ACCACCGTGT GGCCAAGCTG 
CTGGTGGAGA AGGGAGCCAA GCCCAACTCC AGAGCTCTGA ATGGCTTCAC GCCTCTCCAC 
ATTGCCTGCA AGAAGAACCA CATTCGGGTG ATGGAGTTGC TTCTGAAGAC AGGAGCCTCC 
ATTGATGCTG TCACAGAGTC TGGCCTGACC CCCTTGCATG TGGCTGCCTT CATGGGGCAC 
TTGCCCATTG TCAAAACCTT GCTGCAGCGT GGAGCATCTC CTAATGTGTC CAATGTGAAA 
GTGGAGACTC CCCTGCACAT GGCAGCCAGA GCTGGGCACA TGGATGTGGC CAAGTATCTG 
ATACAGAACA AGGCCAAAAT CAACGCCAAG GCCAAGGATG ACCAGACTCC TCTGCACTGT 
GCTGCACGCA TTGGCCACAC CAGCATGGTC CAACTCCTGC TGGAGAACAA TGCCGACCCC 
AACCTGGCCA CTACAGCAGG GCACACGCCT CTGCACATCG CTGCCAGAGA GGGGCATGTA 
GACACAGCCT TGGCCCTGCT AGAGAAGGGA GCCTCTCAGA CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TCCACGTTGC AGCCAAGTAT GGGAAGGTGG ATGTGGCAGA GCTGCTGCTG 
GTGCATGATG CTCACCCCAA TGCAGCGGGG AAGAACGGCC TGACCCCACT GCACGTAGCT 
GTGCACCACA ACAACCTGGA GATCGTCAAG CTTCTGCTTC CCAAGGGGAG CTCTCCACAC 
AGCTCAGCCT GGAACGGGTA CACCCCTCTG CACATTGCCG CCAAGCAGAA CCAGATGGAG 
GTGGCCAGCA GCTTGCTGCA GTATGGTGCT TCTGCAAATG CGGAGTCTGT TCAGGGAGTC 
ACCCCTCTGC ACCTGGCTTC TCAGGAAGGG CATACAGACA TGGTAGCACT GCTTTTCTCC 
AAGCAAGCCA ATGGAAACTT AGGCAACAAG AGTGGTCTGA CTCCTCTCCA TCTCGTGGCC 
CAAGAGGGAC ACGTACCAGT GGCTGATGTT CTGGTGAAAC ATGGAGTCAC AGTGGATGCG 
ACAACCAGGA TGGGCTACAC CTCACTGCAC ATAGCCAGCC ACTATGGGAA CATCAAGTTG 
GTGAAGTTTC TGCTGCAGCA CCAGGCTGAT GTCAATGCTA AGACTAAGCT GGGCTACACA 
CCCCTGCACC AGGCGGCACA GCAGGGCCAC ACGGATGTGG TGACCCTGCT GCTGAAGCAT 
GGTGCCTCTC CCAACGAGAT CAGCACAAAT GGCACCACTC CCCTGGCCAT TGCGAAGCGC 
CTCGGTTACA TTTCCGTCAC AGATGTGCTC AAGATAGTCA CAGAGGAGAC CACCATCCCA 
CCATTCAGTG ACAAGTACCG CATGAGCTTC CCGGAGACCG TGGATGAGAT TCTGGATGTG 
TCGGAGGATG AAGGCACTGC TCATGTCACA GTAATGGAGG AGGAGCTGGT CCCACCGAAA 
CCCAGAACAC CTGAACCCAA GGAGCAGGAG GGCAAGAGGG AGATGCTGGA GTTTGTGACC 
GTGACAACAC TGGAGCAAAT GGAGGAATCT GCAGCTGTCC CACAGGTCCC CTGCGTGCCC 
CCTGAGACTG TGGTGACCAT AGCAGAGGAG TCTGAGCAGA TGGGACCTGT GGAGACTGAG 
GCTGAGCAAG GCGGCCTGCT GCCTGCACCC TCGGTGTCCC CACAGGAGCC CTCCAAGGAG 
TTCGATGAGG ACTCCTTGAT TCCCAGCAGC CCGGCCACCG AAACCTCGGA CAACATCAGC 
CCCGTGGCCA GCCCCGTTCA CACAGGGTTC CTGGTGAGCT TCATGGTGGA TGCGCGTGGC 
GGCTCCATGC GGGGCAGCCG GCACCACGGG CTGCGCGTGG TCATCCCTCC CCGTGCCTGT 
GCTGCACCCA CCCGCATCAC TTGCCGCCTG GTGAAGCCTC AGAAGCTGCC TGCCCCCCCA 
CCGCTGGCTG AGGAGGAGGG CCTGGCAAGC CGCATCATTG CACTGGGGCC CGCTGGCGCC 
CAGTTCCTCA GCCCCGTCAT TGTGGAGATT CCTCACTTTG CCTCCTATGG ACGTGGGGAC 
CGTGAGCTGG TGGTGTTGCG CAGTGAGAAT GGCTCTGTCT GGAAGGAGCA CCGCAACCGC 
TACGAGGAGA GCTACATGGA CCAACTGCTC AGTGGCATGG ATGAGGAGCT GGAGAGCCAA 
GAGGAGCTGG AGAAGAAGAG GGTCTGCCGC ATCATCACCA CTGACTTTCC TCTCTACTTC 
GTGGTCATGT CCCGGATTTG CCAGGACTGT GACATGATTG GCCCCGAGGG AGGGTGCTTG 
AAAAGCACAC TGGTGCCCAT GGTGCAGGCC ACCTTCCCAG ACACCGCTGT CACCAAGAAA 
GTGAGGCTGG CTCTGCAGGC GCAGCCCGTG CCTGATGAGC TGGTGACCAA GCTGCTGGGG 
AACCAGGCAA CCTTCAGCCC CATCGTCACA GTGGAGCCAC GTCGGAGGAA GTTCCACCGC 
CCCATTGGCC TCCGCATCCC TCTGCCCCCA TCCTGGAAGG ACAATCCCCG GGACAGCGGC 
GAGGGTGACA CCACCAGCCT GCGCCTGCTC TGTTCTGTGA TAGGAGGGAC AGCACAAGCT 
CAGTGGGAAG ACATCACAGG CACCACAAAG CTGGTCTATG AAAAAGAGTG TGCTAACTTT 
ACCACCAATG TGTCTGCCAG GTTCTGGCTG GCTGACTGCC CGCGCACTGC TGAGGCTGTG 
CACTTTGCCA CCGTGCTGTA CAAGGAACTG ACGGCTGTGC CCTACATGGC CAAATTTGTG 
GTGTTTGCTA AGATGAACGA TGGACGGGAA GGCCGGCTGC GCTGCTACTG CATGACTGAT 
GACAAGGTGG ACAAGACTTT GGAGCATCAT GAGAACTTCA CTGAGGTGGC CCGCAGCAGG 
GACATTGAGG TGGTGGAAGG AATGCCTCTG CATGTCGAGC TCTCAGGGAA CCTGGTGCCC 
GTCAAGAAGG CCACGCAGTC ACGCACCTTC TTCTTCCAGT CCTTCCGGGA AAGCCGCCTC 
GCCATCCCCA TCAAGGTCCG GGACAGCAGC CGGGAAGCCA GTGGCTCCCT GTCCTTCCTG 
CGCAAGGCCA TGAAGTATGA GGACTTGCAG CATGTGCTCT GCCACCTCAA CATCAGCATT 
CCACCCTGCA CCAAGGGAAG CAGCACTGAG GAGCGAAGGA GGACACTGAC ACCTTTGTCT 
CTGCGCTCTC TGAGCAGCAC CGACAGGGCA GACCAGAAGA TGATCGACAT TGCAGAGCAG 
CTTGGCCTCA GCTGGGCAGA GCTGGCACGT GAGCTGCAGT TTGGGGTGGA TGACATCAAC 
AGGATTCGTG TGGAGAACCC TAACTCCTTG CTGGAGCAGA GCATAGCCTT GCTTAACCTC 
TGGGTCAGCC GCGAGGGCAA GGGTGTCAAA ATTGAGAACC TGTACACAGC GCTGAGGAAC 
ATTGACCGCA GCGAGATCAT CAGCACGTTG GAGGGCTCCG GCCGGCAGAG CCGCAGCATG 
AAGGGCAGCT GGCGTTACAT GGACAGGGAC TATTCACTCT CGCCCTCCCA GATGAATGGT 
TACGCTTCGC TGCAGGACGA GCTGCTCTCC CCTGCCTCCC TGCATTATAT GCTGCCATCC 
CCTCTGCGTG CTGACCAGTA CTGGAACGAG GTGGCCATCA TGGACGCCAT CCCCATGGCC 
GCCACTGAGC AGGACGCCCT GATGGAGATG TCTGACATGC AGGTGTGGTC CTCAGGGCTC 
ACCCCTTCAC TGGTGACGGC TGAGGACTCC TCTCTGGAGT GCAGCAAGGC TGAGGACTCG 
GATGCTACAA GCGAAGGTCG TTTCCCAGGG CACCTTCTGA CAGATGCACA TGTCCCAGAC 
CACATGGGCT CTATGGACTT AGTTGAGGAT GACACAGTGG ACTCAGACGC CATGAACGGG 
CTGATTGAAC TGCTAGAGCA GGAGGAGGGG CAGAGGCCAG AGGGGAAGAT GCCAGCCAGC 
GACCACCAGC TAGGGACTGG AGAGCAGAAC CCAGAGAGTG AAGTCTCTTT TGTTTCAGTT 
CAACAGAAGG TACAAGCCAC GATCGTGCCA TCGCCAACCG TGAGCCACAC AGTGGAGAGG 
AGTGCAGACA GGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK1 ( XM_015297400.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152886.1 Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X11, mRNA.

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CloneID	OGa45395
Clone ID Related Accession (Same CDS sequence)	XM_015297400.1
Accession Version	XM_015297400.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ankyrin-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455999.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGAGAG GGCATGCACC AACCACTGTG GACCCCTTGC CTTCTTGTTG CAAGCAAGGA 
TCTCCTGAAG GGATGCTGGA GCAGGAGTCT GCTGTGCAGG CTGATGCTGC GACCAGCTTC 
CTGAGAGCTG CAAGATCTGG GAATCTGGAC AGAGCCTTGG ATCATCTCAG GAATGGGGTA 
GATATTAACA CCTGTAACCA GAATGGGCTG AATGCCTTGC ACCTGGCCTC CAAGGAGGGC 
CACACGAAAA TGGTGGTGGA GCTTCTGCAC AAGGAGATTG TTTTGGAGAC CACTACCAAG 
AAGGGAAACA CAGCCCTGCA CATCGCTGCC CTCGCTGGAC AGCAGGACGT GGTCCGGGAA 
CTGGTGAACT ATGGGGCCAA TGTCAATGCT CAGTCGCAGA AGGGCTTCAC ACCACTCTAC 
ATGGCAGCGC AGGAAAACCA CCTGGAAGTT GTCAAATTCC TGCTGGAAAA CGGAGCCAAC 
CAGAATGTAG CCACAGAGGA TGGCTTCACA CCACTAGCTG TGGCTCTGCA GCAGGGTCAT 
GAGAATGTGG TTGCTCACCT TATCAACTAT GGGACGAAGG GTAAGGTCCG CCTGCCTGCC 
TTGCACATTG CAGCTCGCAA CGATGACACC CGTACAGCCG CCGTGCTGCT GCAGAATGAC 
CCCAATGCTG ACGTCCTCTC CAAGACTGGA TTTACACCTT TGCACATTGC AGCCCACTAT 
GAGAATCTCA GCGTGGCCCA ATTATTGCTG AATCGTGGAG CCAGCGTCAA CTTCACACCC 
CAGAATGGGA TCACTCCTTT GCACATAGCC TCTCGTCGAG GCAACATCAT CATGGTACGG 
CTGCTGCTGG ACCGTGGGGC CCAGATAGAG ACAAGGACCA AGGATGAGTT GACGCCTCTC 
CACTGTGCAG CTCGCAATGG ACATGTGCGA ATTGCAGAGA TCCTACTGGA CCATGGTGCT 
CCCATTCAAG CCAAAACAAA GAACGGTCTG TCACCGATCC ACATGGCAGC ACAGGGTGAC 
CACCTGGACT GTGTGCGCCT ACTCCTGCAG TACAGTGCTG AGATAGACGA CATCACCCTG 
GACCACCTGA CACCACTGCA TGTAGCTGCG CACTGTGGCC ACCACCGTGT GGCCAAGCTG 
CTGGTGGAGA AGGGAGCCAA GCCCAACTCC AGAGCTCTGA ATGGCTTCAC GCCTCTCCAC 
ATTGCCTGCA AGAAGAACCA CATTCGGGTG ATGGAGTTGC TTCTGAAGAC AGGAGCCTCC 
ATTGATGCTG TCACAGAGTC TGGCCTGACC CCCTTGCATG TGGCTGCCTT CATGGGGCAC 
TTGCCCATTG TCAAAACCTT GCTGCAGCGT GGAGCATCTC CTAATGTGTC CAATGTGAAA 
GTGGAGACTC CCCTGCACAT GGCAGCCAGA GCTGGGCACA TGGATGTGGC CAAGTATCTG 
ATACAGAACA AGGCCAAAAT CAACGCCAAG GCCAAGGATG ACCAGACTCC TCTGCACTGT 
GCTGCACGCA TTGGCCACAC CAGCATGGTC CAACTCCTGC TGGAGAACAA TGCCGACCCC 
AACCTGGCCA CTACAGCAGG GCACACGCCT CTGCACATCG CTGCCAGAGA GGGGCATGTA 
GACACAGCCT TGGCCCTGCT AGAGAAGGGA GCCTCTCAGA CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TCCACGTTGC AGCCAAGTAT GGGAAGGTGG ATGTGGCAGA GCTGCTGCTG 
GTGCATGATG CTCACCCCAA TGCAGCGGGG AAGAACGGCC TGACCCCACT GCACGTAGCT 
GTGCACCACA ACAACCTGGA GATCGTCAAG CTTCTGCTTC CCAAGGGGAG CTCTCCACAC 
AGCTCAGCCT GGAACGGGTA CACCCCTCTG CACATTGCCG CCAAGCAGAA CCAGATGGAG 
GTGGCCAGCA GCTTGCTGCA GTATGGTGCT TCTGCAAATG CGGAGTCTGT TCAGGGAGTC 
ACCCCTCTGC ACCTGGCTTC TCAGGAAGGG CATACAGACA TGGTAGCACT GCTTTTCTCC 
AAGCAAGCCA ATGGAAACTT AGGCAACAAG AGTGGTCTGA CTCCTCTCCA TCTCGTGGCC 
CAAGAGGGAC ACGTACCAGT GGCTGATGTT CTGGTGAAAC ATGGAGTCAC AGTGGATGCG 
ACAACCAGGA TGGGCTACAC CTCACTGCAC ATAGCCAGCC ACTATGGGAA CATCAAGTTG 
GTGAAGTTTC TGCTGCAGCA CCAGGCTGAT GTCAATGCTA AGACTAAGCT GGGCTACACA 
CCCCTGCACC AGGCGGCACA GCAGGGCCAC ACGGATGTGG TGACCCTGCT GCTGAAGCAT 
GGTGCCTCTC CCAACGAGAT CAGCACAAAT GGCACCACTC CCCTGGCCAT TGCGAAGCGC 
CTCGGTTACA TTTCCGTCAC AGATGTGCTC AAGATAGTCA CAGAGGAGAC CACCATCCCA 
CCATTCAGTG ACAAGTACCG CATGAGCTTC CCGGAGACCG TGGATGAGAT TCTGGATGTG 
TCGGAGGATG AAGGCACTGC TCATGTCACA GTAATGGAGG AGGAGCTGGT CCCACCGAAA 
CCCAGAACAC CTGAACCCAA GGAGCAGGAG GGCAAGAGGG AGATGCTGGA GTTTGTGACC 
GTGACAACAC TGGAGCAAAT GGAGGAATCT GCAGCTGTCC CACAGGTCCC CTGCGTGCCC 
CCTGAGACTG TGGTGACCAT AGCAGAGGAG TCTGAGCAGA TGGGACCTGT GGAGACTGAG 
GCTGAGCAAG GCGGCCTGCT GCCTGCACCC TCGGTGTCCC CACAGGAGCC CTCCAAGGAG 
TTCGATGAGG ACTCCTTGAT TCCCAGCAGC CCGGCCACCG AAACCTCGGA CAACATCAGC 
CCCGTGGCCA GCCCCGTTCA CACAGGGTTC CTGGTGAGCT TCATGGTGGA TGCGCGTGGC 
GGCTCCATGC GGGGCAGCCG GCACCACGGG CTGCGCGTGG TCATCCCTCC CCGTGCCTGT 
GCTGCACCCA CCCGCATCAC TTGCCGCCTG GTGAAGCCTC AGAAGCTGCC TGCCCCCCCA 
CCGCTGGCTG AGGAGGAGGG CCTGGCAAGC CGCATCATTG CACTGGGGCC CGCTGGCGCC 
CAGTTCCTCA GCCCCGTCAT TGTGGAGATT CCTCACTTTG CCTCCTATGG ACGTGGGGAC 
CGTGAGCTGG TGGTGTTGCG CAGTGAGAAT GGCTCTGTCT GGAAGGAGCA CCGCAACCGC 
TACGAGGAGA GCTACATGGA CCAACTGCTC AGTGGCATGG ATGAGGAGCT GGAGAGCCAA 
GAGGAGCTGG AGAAGAAGAG GGTCTGCCGC ATCATCACCA CTGACTTTCC TCTCTACTTC 
GTGGTCATGT CCCGGATTTG CCAGGACTGT GACATGATTG GCCCCGAGGG AGGGTGCTTG 
AAAAGCACAC TGGTGCCCAT GGTGCAGGCC ACCTTCCCAG ACACCGCTGT CACCAAGAAA 
GTGAGGCTGG CTCTGCAGGC GCAGCCCGTG CCTGATGAGC TGGTGACCAA GCTGCTGGGG 
AACCAGGCAA CCTTCAGCCC CATCGTCACA GTGGAGCCAC GTCGGAGGAA GTTCCACCGC 
CCCATTGGCC TCCGCATCCC TCTGCCCCCA TCCTGGAAGG ACAATCCCCG GGACAGCGGC 
GAGGGTGACA CCACCAGCCT GCGCCTGCTC TGTTCTGTGA TAGGAGGGAC AGCACAAGCT 
CAGTGGGAAG ACATCACAGG CACCACAAAG CTGGTCTATG AAAAAGAGTG TGCTAACTTT 
ACCACCAATG TGTCTGCCAG GTTCTGGCTG GCTGACTGCC CGCGCACTGC TGAGGCTGTG 
CACTTTGCCA CCGTGCTGTA CAAGGAACTG ACGGCTGTGC CCTACATGGC CAAATTTGTG 
GTGTTTGCTA AGATGAACGA TGGACGGGAA GGCCGGCTGC GCTGCTACTG CATGACTGAT 
GACAAGGTGG ACAAGACTTT GGAGCATCAT GAGAACTTCA CTGAGGTGGC CCGCAGCAGG 
GACATTGAGG TGGTGGAAGG AATGCCTCTG CATGTCGAGC TCTCAGGGAA CCTGGTGCCC 
GTCAAGAAGG CCACGCAGTC ACGCACCTTC TTCTTCCAGT CCTTCCGGGA AAGCCGCCTC 
GCCATCCCCA TCAAGGTCCG GGACAGCAGC CGGGAAGCCA GTGGCTCCCT GTCCTTCCTG 
CGCAAGGCCA TGAAGTATGA GGACTTGCAG CATGTGCTCT GCCACCTCAA CATCAGCATT 
CCACCCTGCA CCAAGGGAAG CAGCACTGAG GAGCGAAGGA GGACACTGAC ACCTTTGTCT 
CTGCGCTCTC TGAGCAGCAC CGACAGGGCA GACCAGAAGA TGATCGACAT TGCAGAGCAG 
CTTGGCCTCA GCTGGGCAGA GCTGGCACGT GAGCTGCAGT TTGGGGTGGA TGACATCAAC 
AGGATTCGTG TGGAGAACCC TAACTCCTTG CTGGAGCAGA GCATAGCCTT GCTTAACCTC 
TGGGTCAGCC GCGAGGGCAA GGGTGTCAAA ATTGAGAACC TGTACACAGC GCTGAGGAAC 
ATTGACCGCA GCGAGATCAT CAGCACGTTG GAGGGCTCCG GCCGGCAGAG CCGCAGCATG 
AAGGGCAGCT GGCGTTACAT GGACAGGGAC TATTCACTCT CGCCCTCCCA GATGAATGTT 
CAACAGAAGG TACAAGCCAC GATCGTGCCA TCGCCAACCG TGAGCCACAC AGTGGAGAGG 
AGTGCAGACA GGGCAACGAA GTCCTCCATC TCCCTGGGGA GCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152886.1
CDS	892..5799
Translation	MAQAAKQLKKIKDIEAQALQEQKEKEESNRKRRNRSRDRKKKRGHAPTTVDPLPSCCKQGSPEGMLEQESAVQADAATSFLRAARSGNLDRALDHLRNGVDINTCNQNGLNALHLASKEGHTKMVVELLHKEIVLETTTKKGNTALHIAALAGQQDVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLSVAQLLLNRGASVNFTPQNGITPLHIASRRGNIIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRIAEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYSAEIDDITLDHLTPLHVAAHCGHHRVAKLLVEKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVAAFMGHLPIVKTLLQRGASPNVSNVKVETPLHMAARAGHMDVAKYLIQNKAKINAKAKDDQTPLHCAARIGHTSMVQLLLENNADPNLATTAGHTPLHIAAREGHVDTALALLEKGASQTCMTKKGFTPLHVAAKYGKVDVAELLLVHDAHPNAAGKNGLTPLHVAVHHNNLEIVKLLLPKGSSPHSSAWNGYTPLHIAAKQNQMEVASSLLQYGASANAESVQGVTPLHLASQEGHTDMVALLFSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLVKHGVTVDATTRMGYTSLHIASHYGNIKLVKFLLQHQADVNAKTKLGYTPLHQAAQQGHTDVVTLLLKHGASPNEISTNGTTPLAIAKRLGYISVTDVLKIVTEETTIPPFSDKYRMSFPETVDEILDVSEDEGTAHVTVMEEELVPPKPRTPEPKEQEGKREMLEFVTVTTLEQMEESAAVPQVPCVPPETVVTIAEESEQMGPVETEAEQGGLLPAPSVSPQEPSKEFDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHHGLRVVIPPRACAAPTRITCRLVKPQKLPAPPPLAEEEGLASRIIALGPAGAQFLSPVIVEIPHFASYGRGDRELVVLRSENGSVWKEHRNRYEESYMDQLLSGMDEELESQEELEKKRVCRIITTDFPLYFVVMSRICQDCDMIGPEGGCLKSTLVPMVQATFPDTAVTKKVRLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWKDNPRDSGEGDTTSLRLLCSVIGGTAQAQWEDITGTTKLVYEKECANFTTNVSARFWLADCPRTAEAVHFATVLYKELTAVPYMAKFVVFAKMNDGREGRLRCYCMTDDKVDKTLEHHENFTEVARSRDIEVVEGMPLHVELSGNLVPVKKATQSRTFFFQSFRESRLAIPIKVRDSSREASGSLSFLRKAMKYEDLQHVLCHLNISIPPCTKGSSTEERRRTLTPLSLRSLSSTDRADQKMIDIAEQLGLSWAELARELQFGVDDINRIRVENPNSLLEQSIALLNLWVSREGKGVKIENLYTALRNIDRSEIISTLEGSGRQSRSMKGSWRYMDRDYSLSPSQMNVQQKVQATIVPSPTVSHTVERSADRATKSSISLGSM

Target ORF information:

RefSeq Version	XM_015297400.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ankyrin 1, erythrocytic (ANK1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297400.1

ORF Insert Sequence:

ATGGCTCAAG CAGCAAAGCA GCTGAAGAAA ATCAAAGACA TTGAGGCTCA GGCTCTACAG 
GAGCAGAAGG AGAAGGAAGA ATCCAATCGC AAGCGCAGGA ACCGCTCTCG GGACCGGAAG 
AAAAAGAGAG GGCATGCACC AACCACTGTG GACCCCTTGC CTTCTTGTTG CAAGCAAGGA 
TCTCCTGAAG GGATGCTGGA GCAGGAGTCT GCTGTGCAGG CTGATGCTGC GACCAGCTTC 
CTGAGAGCTG CAAGATCTGG GAATCTGGAC AGAGCCTTGG ATCATCTCAG GAATGGGGTA 
GATATTAACA CCTGTAACCA GAATGGGCTG AATGCCTTGC ACCTGGCCTC CAAGGAGGGC 
CACACGAAAA TGGTGGTGGA GCTTCTGCAC AAGGAGATTG TTTTGGAGAC CACTACCAAG 
AAGGGAAACA CAGCCCTGCA CATCGCTGCC CTCGCTGGAC AGCAGGACGT GGTCCGGGAA 
CTGGTGAACT ATGGGGCCAA TGTCAATGCT CAGTCGCAGA AGGGCTTCAC ACCACTCTAC 
ATGGCAGCGC AGGAAAACCA CCTGGAAGTT GTCAAATTCC TGCTGGAAAA CGGAGCCAAC 
CAGAATGTAG CCACAGAGGA TGGCTTCACA CCACTAGCTG TGGCTCTGCA GCAGGGTCAT 
GAGAATGTGG TTGCTCACCT TATCAACTAT GGGACGAAGG GTAAGGTCCG CCTGCCTGCC 
TTGCACATTG CAGCTCGCAA CGATGACACC CGTACAGCCG CCGTGCTGCT GCAGAATGAC 
CCCAATGCTG ACGTCCTCTC CAAGACTGGA TTTACACCTT TGCACATTGC AGCCCACTAT 
GAGAATCTCA GCGTGGCCCA ATTATTGCTG AATCGTGGAG CCAGCGTCAA CTTCACACCC 
CAGAATGGGA TCACTCCTTT GCACATAGCC TCTCGTCGAG GCAACATCAT CATGGTACGG 
CTGCTGCTGG ACCGTGGGGC CCAGATAGAG ACAAGGACCA AGGATGAGTT GACGCCTCTC 
CACTGTGCAG CTCGCAATGG ACATGTGCGA ATTGCAGAGA TCCTACTGGA CCATGGTGCT 
CCCATTCAAG CCAAAACAAA GAACGGTCTG TCACCGATCC ACATGGCAGC ACAGGGTGAC 
CACCTGGACT GTGTGCGCCT ACTCCTGCAG TACAGTGCTG AGATAGACGA CATCACCCTG 
GACCACCTGA CACCACTGCA TGTAGCTGCG CACTGTGGCC ACCACCGTGT GGCCAAGCTG 
CTGGTGGAGA AGGGAGCCAA GCCCAACTCC AGAGCTCTGA ATGGCTTCAC GCCTCTCCAC 
ATTGCCTGCA AGAAGAACCA CATTCGGGTG ATGGAGTTGC TTCTGAAGAC AGGAGCCTCC 
ATTGATGCTG TCACAGAGTC TGGCCTGACC CCCTTGCATG TGGCTGCCTT CATGGGGCAC 
TTGCCCATTG TCAAAACCTT GCTGCAGCGT GGAGCATCTC CTAATGTGTC CAATGTGAAA 
GTGGAGACTC CCCTGCACAT GGCAGCCAGA GCTGGGCACA TGGATGTGGC CAAGTATCTG 
ATACAGAACA AGGCCAAAAT CAACGCCAAG GCCAAGGATG ACCAGACTCC TCTGCACTGT 
GCTGCACGCA TTGGCCACAC CAGCATGGTC CAACTCCTGC TGGAGAACAA TGCCGACCCC 
AACCTGGCCA CTACAGCAGG GCACACGCCT CTGCACATCG CTGCCAGAGA GGGGCATGTA 
GACACAGCCT TGGCCCTGCT AGAGAAGGGA GCCTCTCAGA CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TCCACGTTGC AGCCAAGTAT GGGAAGGTGG ATGTGGCAGA GCTGCTGCTG 
GTGCATGATG CTCACCCCAA TGCAGCGGGG AAGAACGGCC TGACCCCACT GCACGTAGCT 
GTGCACCACA ACAACCTGGA GATCGTCAAG CTTCTGCTTC CCAAGGGGAG CTCTCCACAC 
AGCTCAGCCT GGAACGGGTA CACCCCTCTG CACATTGCCG CCAAGCAGAA CCAGATGGAG 
GTGGCCAGCA GCTTGCTGCA GTATGGTGCT TCTGCAAATG CGGAGTCTGT TCAGGGAGTC 
ACCCCTCTGC ACCTGGCTTC TCAGGAAGGG CATACAGACA TGGTAGCACT GCTTTTCTCC 
AAGCAAGCCA ATGGAAACTT AGGCAACAAG AGTGGTCTGA CTCCTCTCCA TCTCGTGGCC 
CAAGAGGGAC ACGTACCAGT GGCTGATGTT CTGGTGAAAC ATGGAGTCAC AGTGGATGCG 
ACAACCAGGA TGGGCTACAC CTCACTGCAC ATAGCCAGCC ACTATGGGAA CATCAAGTTG 
GTGAAGTTTC TGCTGCAGCA CCAGGCTGAT GTCAATGCTA AGACTAAGCT GGGCTACACA 
CCCCTGCACC AGGCGGCACA GCAGGGCCAC ACGGATGTGG TGACCCTGCT GCTGAAGCAT 
GGTGCCTCTC CCAACGAGAT CAGCACAAAT GGCACCACTC CCCTGGCCAT TGCGAAGCGC 
CTCGGTTACA TTTCCGTCAC AGATGTGCTC AAGATAGTCA CAGAGGAGAC CACCATCCCA 
CCATTCAGTG ACAAGTACCG CATGAGCTTC CCGGAGACCG TGGATGAGAT TCTGGATGTG 
TCGGAGGATG AAGGCACTGC TCATGTCACA GTAATGGAGG AGGAGCTGGT CCCACCGAAA 
CCCAGAACAC CTGAACCCAA GGAGCAGGAG GGCAAGAGGG AGATGCTGGA GTTTGTGACC 
GTGACAACAC TGGAGCAAAT GGAGGAATCT GCAGCTGTCC CACAGGTCCC CTGCGTGCCC 
CCTGAGACTG TGGTGACCAT AGCAGAGGAG TCTGAGCAGA TGGGACCTGT GGAGACTGAG 
GCTGAGCAAG GCGGCCTGCT GCCTGCACCC TCGGTGTCCC CACAGGAGCC CTCCAAGGAG 
TTCGATGAGG ACTCCTTGAT TCCCAGCAGC CCGGCCACCG AAACCTCGGA CAACATCAGC 
CCCGTGGCCA GCCCCGTTCA CACAGGGTTC CTGGTGAGCT TCATGGTGGA TGCGCGTGGC 
GGCTCCATGC GGGGCAGCCG GCACCACGGG CTGCGCGTGG TCATCCCTCC CCGTGCCTGT 
GCTGCACCCA CCCGCATCAC TTGCCGCCTG GTGAAGCCTC AGAAGCTGCC TGCCCCCCCA 
CCGCTGGCTG AGGAGGAGGG CCTGGCAAGC CGCATCATTG CACTGGGGCC CGCTGGCGCC 
CAGTTCCTCA GCCCCGTCAT TGTGGAGATT CCTCACTTTG CCTCCTATGG ACGTGGGGAC 
CGTGAGCTGG TGGTGTTGCG CAGTGAGAAT GGCTCTGTCT GGAAGGAGCA CCGCAACCGC 
TACGAGGAGA GCTACATGGA CCAACTGCTC AGTGGCATGG ATGAGGAGCT GGAGAGCCAA 
GAGGAGCTGG AGAAGAAGAG GGTCTGCCGC ATCATCACCA CTGACTTTCC TCTCTACTTC 
GTGGTCATGT CCCGGATTTG CCAGGACTGT GACATGATTG GCCCCGAGGG AGGGTGCTTG 
AAAAGCACAC TGGTGCCCAT GGTGCAGGCC ACCTTCCCAG ACACCGCTGT CACCAAGAAA 
GTGAGGCTGG CTCTGCAGGC GCAGCCCGTG CCTGATGAGC TGGTGACCAA GCTGCTGGGG 
AACCAGGCAA CCTTCAGCCC CATCGTCACA GTGGAGCCAC GTCGGAGGAA GTTCCACCGC 
CCCATTGGCC TCCGCATCCC TCTGCCCCCA TCCTGGAAGG ACAATCCCCG GGACAGCGGC 
GAGGGTGACA CCACCAGCCT GCGCCTGCTC TGTTCTGTGA TAGGAGGGAC AGCACAAGCT 
CAGTGGGAAG ACATCACAGG CACCACAAAG CTGGTCTATG AAAAAGAGTG TGCTAACTTT 
ACCACCAATG TGTCTGCCAG GTTCTGGCTG GCTGACTGCC CGCGCACTGC TGAGGCTGTG 
CACTTTGCCA CCGTGCTGTA CAAGGAACTG ACGGCTGTGC CCTACATGGC CAAATTTGTG 
GTGTTTGCTA AGATGAACGA TGGACGGGAA GGCCGGCTGC GCTGCTACTG CATGACTGAT 
GACAAGGTGG ACAAGACTTT GGAGCATCAT GAGAACTTCA CTGAGGTGGC CCGCAGCAGG 
GACATTGAGG TGGTGGAAGG AATGCCTCTG CATGTCGAGC TCTCAGGGAA CCTGGTGCCC 
GTCAAGAAGG CCACGCAGTC ACGCACCTTC TTCTTCCAGT CCTTCCGGGA AAGCCGCCTC 
GCCATCCCCA TCAAGGTCCG GGACAGCAGC CGGGAAGCCA GTGGCTCCCT GTCCTTCCTG 
CGCAAGGCCA TGAAGTATGA GGACTTGCAG CATGTGCTCT GCCACCTCAA CATCAGCATT 
CCACCCTGCA CCAAGGGAAG CAGCACTGAG GAGCGAAGGA GGACACTGAC ACCTTTGTCT 
CTGCGCTCTC TGAGCAGCAC CGACAGGGCA GACCAGAAGA TGATCGACAT TGCAGAGCAG 
CTTGGCCTCA GCTGGGCAGA GCTGGCACGT GAGCTGCAGT TTGGGGTGGA TGACATCAAC 
AGGATTCGTG TGGAGAACCC TAACTCCTTG CTGGAGCAGA GCATAGCCTT GCTTAACCTC 
TGGGTCAGCC GCGAGGGCAA GGGTGTCAAA ATTGAGAACC TGTACACAGC GCTGAGGAAC 
ATTGACCGCA GCGAGATCAT CAGCACGTTG GAGGGCTCCG GCCGGCAGAG CCGCAGCATG 
AAGGGCAGCT GGCGTTACAT GGACAGGGAC TATTCACTCT CGCCCTCCCA GATGAATGTT 
CAACAGAAGG TACAAGCCAC GATCGTGCCA TCGCCAACCG TGAGCCACAC AGTGGAGAGG 
AGTGCAGACA GGGCAACGAA GTCCTCCATC TCCCTGGGGA GCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ANK1
Entrez Gene ID	396311
Full Name	ankyrin 1
Synonyms	CHANK1
General protein information	Preferred Names ankyrin 1 Names ankyrin-1 ankyrin 1, erythrocytic erythroid ankyrin
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Canis lupus familiaris (dog)	ANK1	XP_005629869.1
Bos taurus (cattle)	ANK1	XP_002698771.3
Mus musculus (house mouse)	Ank1	NP_001104253.1
Homo sapiens (human)	ANK1	NP_001135918.1
Rattus norvegicus (Norway rat)	Ank1	NP_001100792.1
Pan troglodytes (chimpanzee)	ANK1	XP_003311743.1
	ANK1	XP_001099591.2
Gallus gallus (chicken)	ANK1	XP_424401.4
Danio rerio (zebrafish)	si:ch211-263m18.3	XP_005161295.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ANK1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

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