The following BRAF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OGa142100 | |
| Clone ID Related Accession (Same CDS sequence) | XM_025146843.1 | |
| Accession Version | XM_025146843.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2430bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-16 | |
| Organism | Gallus gallus(chicken) | |
| Product | serine/threonine-protein kinase B-raf isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCGGCGC TGAGCAGCGG CAGCAGCGCC GAGGGGGCCT CGCTCTTCAA CGGGGACATG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_025002611.1 |
| CDS | 188..2617 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_025146843.1 |
1 | ATGGCGGCGC TGAGCAGCGG CAGCAGCGCC GAGGGGGCCT CGCTCTTCAA CGGGGACATG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa142101 | |
| Clone ID Related Accession (Same CDS sequence) | XM_025146848.1 | |
| Accession Version | XM_025146848.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2418bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-16 | |
| Organism | Gallus gallus(chicken) | |
| Product | serine/threonine-protein kinase B-raf isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCGGCGC TGAGCAGCGG CAGCAGCGCC GAGGGGGCCT CGCTCTTCAA CGGGGACATG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_025002616.1 |
| CDS | 188..2605 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_025146848.1 |
1 | ATGGCGGCGC TGAGCAGCGG CAGCAGCGCC GAGGGGGCCT CGCTCTTCAA CGGGGACATG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa29296 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015284541.1 | |
| Accession Version | XM_015284541.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-01-03 | |
| Organism | Gallus gallus(chicken) | |
| Product | serine/threonine-protein kinase B-raf isoform X4 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455714.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGATTAAAT TAACACAAGA ACATATAGAA GCGCTGTTAG ACAAGTTTGG AGGAGAGCAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015140027.1 |
| CDS | 38..2185 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015284541.1 |
1 | ATGATTAAAT TAACACAAGA ACATATAGAA GCGCTGTTAG ACAAGTTTGG AGGAGAGCAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa26752 | |
| Clone ID Related Accession (Same CDS sequence) | NM_205302.1 | |
| Accession Version | NM_205302.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-25 | |
| Organism | Gallus gallus(chicken) | |
| Product | serine/threonine-protein kinase B-raf | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X67052.1. ##Evidence-Data-START## Transcript exon combination :: X67052.1 [ECO:0000332] ##Evidence-Data-END## | |
1 | ATGGCGGCGC TGAGCAGCGG CAGCAGCGCC GAGGGGGCCT CGCTCTTCAA CGGGGACATG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_990633.1 |
| CDS | 34..2454 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_205302.1 |
1 | ATGGCGGCGC TGAGCAGCGG CAGCAGCGCC GAGGGGGCCT CGCTCTTCAA CGGGGACATG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa29293 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015284526.1 , XM_015284526.2 | |
| Accession Version | XM_015284526.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-01-03 | |
| Organism | Gallus gallus(chicken) | |
| Product | serine/threonine-protein kinase B-raf isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455714.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCGGCGC TGAGCAGCGG CAGCAGCGCC GAGGGGGCCT CGCTCTTCAA CGGGGACATG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015140012.1 |
| CDS | 183..2486 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015284526.1 |
1 | ATGGCGGCGC TGAGCAGCGG CAGCAGCGCC GAGGGGGCCT CGCTCTTCAA CGGGGACATG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa29293 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015284526.1 , XM_015284526.2 | |
| Accession Version | XM_015284526.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-16 | |
| Organism | Gallus gallus(chicken) | |
| Product | serine/threonine-protein kinase B-raf isoform X3 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284526.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCGGCGC TGAGCAGCGG CAGCAGCGCC GAGGGGGCCT CGCTCTTCAA CGGGGACATG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015140012.1 |
| CDS | 186..2489 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015284526.2 |
1 | ATGGCGGCGC TGAGCAGCGG CAGCAGCGCC GAGGGGGCCT CGCTCTTCAA CGGGGACATG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa29294 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015284532.1 | |
| Accession Version | XM_015284532.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-01-03 | |
| Organism | Gallus gallus(chicken) | |
| Product | serine/threonine-protein kinase B-raf isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455714.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCGGCGC TGAGCAGCGG CAGCAGCGCC GAGGGGGCCT CGCTCTTCAA CGGGGACATG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015140018.1 |
| CDS | 184..2484 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015284532.1 |
1 | ATGGCGGCGC TGAGCAGCGG CAGCAGCGCC GAGGGGGCCT CGCTCTTCAA CGGGGACATG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa29295 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015284536.2 , XM_015284536.1 | |
| Accession Version | XM_015284536.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-16 | |
| Organism | Gallus gallus(chicken) | |
| Product | serine/threonine-protein kinase B-raf isoform X4 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284536.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCGGCGC TGAGCAGCGG CAGCAGCGCC GAGGGGGCCT CGCTCTTCAA CGGGGACATG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015140022.1 |
| CDS | 186..2477 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015284536.2 |
1 | ATGGCGGCGC TGAGCAGCGG CAGCAGCGCC GAGGGGGCCT CGCTCTTCAA CGGGGACATG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa29295 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015284536.2 , XM_015284536.1 | |
| Accession Version | XM_015284536.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-01-03 | |
| Organism | Gallus gallus(chicken) | |
| Product | serine/threonine-protein kinase B-raf isoform X3 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455714.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCGGCGC TGAGCAGCGG CAGCAGCGCC GAGGGGGCCT CGCTCTTCAA CGGGGACATG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015140022.1 |
| CDS | 184..2475 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015284536.1 |
1 | ATGGCGGCGC TGAGCAGCGG CAGCAGCGCC GAGGGGGCCT CGCTCTTCAA CGGGGACATG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
B-raf alternative splicing is dispensable for development but required for learning and memory associated with the hippocampus in the adult mouse. |
Functional analysis of the regulatory requirements of B-Raf and the B-Raf(V600E) oncoprotein. |
Identification of novel ERK-mediated feedback phosphorylation sites at the C-terminus of B-Raf. |
Inducible gene deletion reveals different roles for B-Raf and Raf-1 in B-cell antigen receptor signalling. |
Genomic organization and nucleotide sequence of the coding region of the chicken c-Rmil(B-raf-1) proto-oncogene. |
