ZEB1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZEB1
Entrez Gene ID	396029
Full Name	zinc finger E-box binding homeobox 1
Synonyms	TCF8
General protein information	Preferred Names zinc finger E-box binding homeobox 1 Names zinc finger E-box-binding homeobox 1 TCF-8 delta EF1 delta-crystallin enhancer-binding factor deltaEF1 transcription factor 8 (represses interleukin 2 expression)
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015281304.1	XP_015136790.1	zinc finger E-box-binding homeobox 1 isoform X1
NM_205131.1	NP_990462.1	zinc finger E-box-binding homeobox 1
XM_015281305.1	XP_015136791.1	zinc finger E-box-binding homeobox 1 isoform X3
XM_015281304.2	XP_015136790.1	zinc finger E-box-binding homeobox 1 isoform X1
XM_025147055.1	XP_025002823.1	zinc finger E-box-binding homeobox 1 isoform X2
XM_025147056.1	XP_025002824.1	zinc finger E-box-binding homeobox 1 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	ZEB1	NP_001167567.1
Bos taurus (cattle)	ZEB1	NP_001193519.1
Mus musculus (house mouse)	Zeb1	NP_035676.1
Gallus gallus (chicken)	ZEB1	NP_990462.1
Canis lupus familiaris (dog)	ZEB1	XP_003638927.1
	ZEB1	XP_001089463.2
Xenopus tropicalis (tropical clawed frog)	zeb1	NP_001015808.1
Pan troglodytes (chimpanzee)	ZEB1	XP_003312560.1
Rattus norvegicus (Norway rat)	Zeb1	NP_037296.1
Danio rerio (zebrafish)	zeb1b	NP_571784.1

Gene Ontology
Bibliography

Related articles in PubMed

miRNA-223 upregulated by MYOD inhibits myoblast proliferation by repressing IGF2 and facilitates myoblast differentiation by inhibiting ZEB1.
Li G, Luo W, Abdalla BA, Ouyang H, Yu J, Hu F, Nie Q, Zhang X
Cell death & disease8(10)e3094(2017 Oct)

Regulation of trunk neural crest delamination by ?EF1 and Sip1 in the chicken embryo.
Yasumi T, Inoue M, Maruhashi M, Kamachi Y, Higashi Y, Kondoh H, Uchikawa M
Development, growth & differentiation58(2)205-14(2016 Feb)

MicroRNA-200C and -150 play an important role in endothelial cell differentiation and vasculogenesis by targeting transcription repressor ZEB1.
Luo Z, Wen G, Wang G, Pu X, Ye S, Xu Q, Wang W, Xiao Q
Stem cells (Dayton, Ohio)31(9)1749-62(2013 Sep)

Negative modulation of the chicken infectious anemia virus promoter by COUP-TF1 and an E box-like element at the transcription start site binding deltaEF1.
Miller MM, Jarosinski KW, Schat KA
The Journal of general virology89(Pt 12)2998-3003(2008 Dec)

Organization of the gene encoding transcriptional repressor deltaEF1 and cross-species conservation of its domains.
Sekido R, Takagi T, Okanami M, Moribe H, Yamamura M, Higashi Y, Kondoh H
Gene173(2)227-32(1996 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Expression of Sox10, the major transcription factor that regulates neural crest development, was also decreased by the shRNAs against deltaEF1 or Sip1.
Title: Regulation of trunk neural crest delamination by ?EF1 and Sip1 in the chicken embryo.

miR-150 and miR-200c play an important role in human endothelial lineage specification and chick embryonic vasculogenesis by targeting ZEB1.
Title: MicroRNA-200C and -150 play an important role in endothelial cell differentiation and vasculogenesis by targeting transcription repressor ZEB1.

These findings indicate that the chicken infectious anemia virus promoter activity can be affected directly or indirectly by COUP-TF1 and deltaEF1.
Title: Negative modulation of the chicken infectious anemia virus promoter by COUP-TF1 and an E box-like element at the transcription start site binding deltaEF1.

The following ZEB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZEB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa31495	XM_015281305.1 Latest version!	Gallus gallus zinc finger E-box binding homeobox 1 (ZEB1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OGa31495	XM_025147056.1 Latest version!	Gallus gallus zinc finger E-box binding homeobox 1 (ZEB1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OGa145753	XM_025147055.1 Latest version!	Gallus gallus zinc finger E-box binding homeobox 1 (ZEB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa31494	XM_015281304.2 Latest version!	Gallus gallus zinc finger E-box binding homeobox 1 (ZEB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa00405	NM_205131.1 Latest version!	Gallus gallus zinc finger E-box binding homeobox 1 (ZEB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OGa31494	XM_015281304.1	Gallus gallus zinc finger E-box binding homeobox 1 (ZEB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZEB1 ( XM_015281305.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015136791.1 Gallus gallus zinc finger E-box binding homeobox 1 (ZEB1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OGa31495
Clone ID Related Accession (Same CDS sequence)	XM_015281305.1 , XM_025147056.1
Accession Version	XM_015281305.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	zinc finger E-box-binding homeobox 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTCGC ACAAGTCGGG ACGAGATCAG AGACATGTGA CGCAGTCCAG TGGTAATCGA 
AAATTCAAGT GCACTGAATG TGGAAAAGCT TTCAAATATA AACATCATCT AAAGGAACAC 
CTACGAATCC ACAGTGGAGA GAAGCCATAT GAGTGCCCAA ACTGCAAGAA ACGTTTTTCC 
CATTCTGGTT CATACAGCTC ACACATAAGC AGTAAGAAGT GTATTGGTTT GATGCCCGTG 
AATGGTCGAG CTCGGTCAGG GCTCAAGATG TCTCAGTGCT CCTCCCCTTC CCTTTCTGCA 
TCGCCCGGTA GCCCAGCAAG ACCACAGATA CGACAAAAGA TAGAAAATAA ACCCTTGCAA 
GAGCAACTTC CTGTTAACCA AATTAAAACT GAACCTGTGG ATTATGAATT CAAGCCCATA 
GTGGTTGCTT CAGGAATTAA TTGTTCGACC CCTTTGCAGA ATGGGGTTTT TAGTGGTGGT 
AGCCCGTTGC AGGCAACCAG TTCTCCTCAG GGTGTGGTGC AAGCTGTTGT TCTGCCAACA 
GTGGGTCTGG TGTCTCCCAT AAGCATCAAC TTAAGTGACA TTCAAAATGT ACTTAAAGTG 
GCAGTGGATG GTAATGTAAT AAGGCAAGTA TTGGAAAACA ATCACGCTAA TCTTGCATCC 
AAAGAACAAG AAACAATCAG CAATGCATCT ATACAACAAG CTGGCCATTC CCTCATTTCA 
GCTATCAGTC TTCCTTTGGT TGACCAAGAT GGGACAACCA AAATTATCAT CAACTACAGC 
TTGGAGCAGC CAAGTCAACT TCAGGTTGTT CCCCAAAATC TAAAAAAAGA ACATTCTGTT 
CCTACAAACA GTTGCAAAAA TGAAAAGTTA CCAGAAGATC TTACGGTGAA GTCTGAGAAA 
GATAAAAACT TTGAAGGAGA GACCAACGAT AGCACTTGTC TTCTTTGTGA TGACTGTCCA 
GGAGATCTTA ATGCACTTCA AGAATTAAAG CACTATGAAA CAAAAAATCC TCCTCAGCTT 
CCCCAGTCCA GTGGAACAGA AGCTGAGAAG CCCAGCTCCC CTGCCCCATC AGAAACTGGG 
GAGAACAACT TATCTCCTGG TCAGCCACCC TTAAAGAACC TTTTATCGCT CCTAAAAGCA 
TATTATGCAT TAAATGCACA ACCAAGCGCA GAAGAGCTTT CAAAAATTGC CGATTCCGTG 
AACCTACCAC TGGATGTGGT AAAAAAGTGG TTTGAAAAAA TGCAAGCTGG ACAAATTTCT 
GTGCAGTCTT CTGGACCATC TTCTCCTGAA CAAGTTAAAA TAAGCAGTCC CACAGATAAC 
GATGATCAAG CAGCAACTAC AAATGAGAGT GAACCCCAGA ACAGCACAAA CAACTCACAA 
AATCCGGCCA ATACAAGTAA ATCTCAGACT TCATCAGGGG GATCAACTCA GAATGGTTCG 
CGCAGTAGCA CGCCATCCCC ATCACCACTA AACCTTTCTT CATCAAGAAA TTCACAGGGT 
TATACGTACA CAGCAGAGGG TGTACAAGAA GAGCCACAAA TGGAACCTCT TGACCTTTCA 
CTACCAAAGC AACATGGAGA ACTGTTGGAA AGATCTACCA TAACTAGTGT TTACCAGAAC 
AGTGTTTATT CTGTCCAGGA AGAACCTTTG AACTTAACTT GTGCAAAAAA AGAACCACAA 
AAGGACAACA GTATTACAGA CTCTGATCCT ATTGTAAATG TAATCCCACC AAGTGCCAAT 
CCCATAAATA TTGCTATACC TACAGTCACT GCCCAGTTAC CTACAATTGT TGCCATTGCT 
GACCAGAACA GTGTTCCATG CTTGAGAGCT CTTGCTGCCA ATAAGCAAAC CATTTTGATT 
CCCCAGGTGG CTTATACGTA CTCTACTACA GTTAGTCCTG CAGTTCAGGA AACACCACCA 
AAACAGACCC AAGCCAACGG AAGTCAGGAC GAAAGGCAGG ACACTAGCTC AGAAGGAGTA 
TCGAATGTAG AAGATCAAAA TGATTCTGAT TCAACACCCC CAAAGAAAAA GATGAGAAAG 
ACAGAAAATG GGATGTATGC ATGTGATTTG TGTGACAAAA TATTCCAGAA GAGCAGCTCA 
TTATTGAGAC ATAAGTATGA ACACACAGGT AAAAGACCTC ATGAGTGTGG AATCTGTAAA 
AAAGCCTTCA AACACAAACA CCATTTGATC GAACACATGC GACTGCATTC TGGGGAGAAA 
CCCTACCAAT GTGACAAATG CGGGAAGCGC TTCTCACACT CGGGGTCGTA CTCTCAGCAC 
ATGAATCATC GCTACTCCTA TTGCAAAAGA GAGGCAGAGG AGCGTGACAG CACAGAGCAG 
GAGGAGGTGG GACAGGAGGT GCTCAGCAGC GAGCATGCTG GTGCCAGGGC ATCGCCATCG 
CAGATCGACT CCGATGAGAG AGAGAGTCTA ACCAGGGAAG AAGAGGAAGA TAGTGAAAAA 
GAGGAAGAGG AGGAGGAAGA AAAGGACGTA GAAGGACTTC AGGAAGAAAA AGAATGTAGA 
AAACTACAAG ATGTAGAGGA GGAAGAAGAA GTAGAAGAAG AAGAAGAAGA AGAGGAAGGG 
AAAACTGAAG GTAACAAGAA CGATGATGTT GTAAATCGAG CAAGCAATGC AGAACCAGAA 
GTTATACAGA GCAATGGGCA GGTGTCAGAA GAAAAAACAA ATAAAGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015136791.1
CDS	707..3397
Translation	MTSHKSGRDQRHVTQSSGNRKFKCTECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCIGLMPVNGRARSGLKMSQCSSPSLSASPGSPARPQIRQKIENKPLQEQLPVNQIKTEPVDYEFKPIVVASGINCSTPLQNGVFSGGSPLQATSSPQGVVQAVVLPTVGLVSPISINLSDIQNVLKVAVDGNVIRQVLENNHANLASKEQETISNASIQQAGHSLISAISLPLVDQDGTTKIIINYSLEQPSQLQVVPQNLKKEHSVPTNSCKNEKLPEDLTVKSEKDKNFEGETNDSTCLLCDDCPGDLNALQELKHYETKNPPQLPQSSGTEAEKPSSPAPSETGENNLSPGQPPLKNLLSLLKAYYALNAQPSAEELSKIADSVNLPLDVVKKWFEKMQAGQISVQSSGPSSPEQVKISSPTDNDDQAATTNESEPQNSTNNSQNPANTSKSQTSSGGSTQNGSRSSTPSPSPLNLSSSRNSQGYTYTAEGVQEEPQMEPLDLSLPKQHGELLERSTITSVYQNSVYSVQEEPLNLTCAKKEPQKDNSITDSDPIVNVIPPSANPINIAIPTVTAQLPTIVAIADQNSVPCLRALAANKQTILIPQVAYTYSTTVSPAVQETPPKQTQANGSQDERQDTSSEGVSNVEDQNDSDSTPPKKKMRKTENGMYACDLCDKIFQKSSSLLRHKYEHTGKRPHECGICKKAFKHKHHLIEHMRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKREAEERDSTEQEEVGQEVLSSEHAGARASPSQIDSDERESLTREEEEDSEKEEEEEEEKDVEGLQEEKECRKLQDVEEEEEVEEEEEEEEGKTEGNKNDDVVNRASNAEPEVIQSNGQVSEEKTNKA

Target ORF information:

RefSeq Version	XM_015281305.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus zinc finger E-box binding homeobox 1 (ZEB1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281305.1

ORF Insert Sequence:

ATGACGTCGC ACAAGTCGGG ACGAGATCAG AGACATGTGA CGCAGTCCAG TGGTAATCGA 
AAATTCAAGT GCACTGAATG TGGAAAAGCT TTCAAATATA AACATCATCT AAAGGAACAC 
CTACGAATCC ACAGTGGAGA GAAGCCATAT GAGTGCCCAA ACTGCAAGAA ACGTTTTTCC 
CATTCTGGTT CATACAGCTC ACACATAAGC AGTAAGAAGT GTATTGGTTT GATGCCCGTG 
AATGGTCGAG CTCGGTCAGG GCTCAAGATG TCTCAGTGCT CCTCCCCTTC CCTTTCTGCA 
TCGCCCGGTA GCCCAGCAAG ACCACAGATA CGACAAAAGA TAGAAAATAA ACCCTTGCAA 
GAGCAACTTC CTGTTAACCA AATTAAAACT GAACCTGTGG ATTATGAATT CAAGCCCATA 
GTGGTTGCTT CAGGAATTAA TTGTTCGACC CCTTTGCAGA ATGGGGTTTT TAGTGGTGGT 
AGCCCGTTGC AGGCAACCAG TTCTCCTCAG GGTGTGGTGC AAGCTGTTGT TCTGCCAACA 
GTGGGTCTGG TGTCTCCCAT AAGCATCAAC TTAAGTGACA TTCAAAATGT ACTTAAAGTG 
GCAGTGGATG GTAATGTAAT AAGGCAAGTA TTGGAAAACA ATCACGCTAA TCTTGCATCC 
AAAGAACAAG AAACAATCAG CAATGCATCT ATACAACAAG CTGGCCATTC CCTCATTTCA 
GCTATCAGTC TTCCTTTGGT TGACCAAGAT GGGACAACCA AAATTATCAT CAACTACAGC 
TTGGAGCAGC CAAGTCAACT TCAGGTTGTT CCCCAAAATC TAAAAAAAGA ACATTCTGTT 
CCTACAAACA GTTGCAAAAA TGAAAAGTTA CCAGAAGATC TTACGGTGAA GTCTGAGAAA 
GATAAAAACT TTGAAGGAGA GACCAACGAT AGCACTTGTC TTCTTTGTGA TGACTGTCCA 
GGAGATCTTA ATGCACTTCA AGAATTAAAG CACTATGAAA CAAAAAATCC TCCTCAGCTT 
CCCCAGTCCA GTGGAACAGA AGCTGAGAAG CCCAGCTCCC CTGCCCCATC AGAAACTGGG 
GAGAACAACT TATCTCCTGG TCAGCCACCC TTAAAGAACC TTTTATCGCT CCTAAAAGCA 
TATTATGCAT TAAATGCACA ACCAAGCGCA GAAGAGCTTT CAAAAATTGC CGATTCCGTG 
AACCTACCAC TGGATGTGGT AAAAAAGTGG TTTGAAAAAA TGCAAGCTGG ACAAATTTCT 
GTGCAGTCTT CTGGACCATC TTCTCCTGAA CAAGTTAAAA TAAGCAGTCC CACAGATAAC 
GATGATCAAG CAGCAACTAC AAATGAGAGT GAACCCCAGA ACAGCACAAA CAACTCACAA 
AATCCGGCCA ATACAAGTAA ATCTCAGACT TCATCAGGGG GATCAACTCA GAATGGTTCG 
CGCAGTAGCA CGCCATCCCC ATCACCACTA AACCTTTCTT CATCAAGAAA TTCACAGGGT 
TATACGTACA CAGCAGAGGG TGTACAAGAA GAGCCACAAA TGGAACCTCT TGACCTTTCA 
CTACCAAAGC AACATGGAGA ACTGTTGGAA AGATCTACCA TAACTAGTGT TTACCAGAAC 
AGTGTTTATT CTGTCCAGGA AGAACCTTTG AACTTAACTT GTGCAAAAAA AGAACCACAA 
AAGGACAACA GTATTACAGA CTCTGATCCT ATTGTAAATG TAATCCCACC AAGTGCCAAT 
CCCATAAATA TTGCTATACC TACAGTCACT GCCCAGTTAC CTACAATTGT TGCCATTGCT 
GACCAGAACA GTGTTCCATG CTTGAGAGCT CTTGCTGCCA ATAAGCAAAC CATTTTGATT 
CCCCAGGTGG CTTATACGTA CTCTACTACA GTTAGTCCTG CAGTTCAGGA AACACCACCA 
AAACAGACCC AAGCCAACGG AAGTCAGGAC GAAAGGCAGG ACACTAGCTC AGAAGGAGTA 
TCGAATGTAG AAGATCAAAA TGATTCTGAT TCAACACCCC CAAAGAAAAA GATGAGAAAG 
ACAGAAAATG GGATGTATGC ATGTGATTTG TGTGACAAAA TATTCCAGAA GAGCAGCTCA 
TTATTGAGAC ATAAGTATGA ACACACAGGT AAAAGACCTC ATGAGTGTGG AATCTGTAAA 
AAAGCCTTCA AACACAAACA CCATTTGATC GAACACATGC GACTGCATTC TGGGGAGAAA 
CCCTACCAAT GTGACAAATG CGGGAAGCGC TTCTCACACT CGGGGTCGTA CTCTCAGCAC 
ATGAATCATC GCTACTCCTA TTGCAAAAGA GAGGCAGAGG AGCGTGACAG CACAGAGCAG 
GAGGAGGTGG GACAGGAGGT GCTCAGCAGC GAGCATGCTG GTGCCAGGGC ATCGCCATCG 
CAGATCGACT CCGATGAGAG AGAGAGTCTA ACCAGGGAAG AAGAGGAAGA TAGTGAAAAA 
GAGGAAGAGG AGGAGGAAGA AAAGGACGTA GAAGGACTTC AGGAAGAAAA AGAATGTAGA 
AAACTACAAG ATGTAGAGGA GGAAGAAGAA GTAGAAGAAG AAGAAGAAGA AGAGGAAGGG 
AAAACTGAAG GTAACAAGAA CGATGATGTT GTAAATCGAG CAAGCAATGC AGAACCAGAA 
GTTATACAGA GCAATGGGCA GGTGTCAGAA GAAAAAACAA ATAAAGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZEB1 ( XM_025147056.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025002824.1 Gallus gallus zinc finger E-box binding homeobox 1 (ZEB1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa31495
Clone ID Related Accession (Same CDS sequence)	XM_015281305.1 , XM_025147056.1
Accession Version	XM_025147056.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	zinc finger E-box-binding homeobox 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTCGC ACAAGTCGGG ACGAGATCAG AGACATGTGA CGCAGTCCAG TGGTAATCGA 
AAATTCAAGT GCACTGAATG TGGAAAAGCT TTCAAATATA AACATCATCT AAAGGAACAC 
CTACGAATCC ACAGTGGAGA GAAGCCATAT GAGTGCCCAA ACTGCAAGAA ACGTTTTTCC 
CATTCTGGTT CATACAGCTC ACACATAAGC AGTAAGAAGT GTATTGGTTT GATGCCCGTG 
AATGGTCGAG CTCGGTCAGG GCTCAAGATG TCTCAGTGCT CCTCCCCTTC CCTTTCTGCA 
TCGCCCGGTA GCCCAGCAAG ACCACAGATA CGACAAAAGA TAGAAAATAA ACCCTTGCAA 
GAGCAACTTC CTGTTAACCA AATTAAAACT GAACCTGTGG ATTATGAATT CAAGCCCATA 
GTGGTTGCTT CAGGAATTAA TTGTTCGACC CCTTTGCAGA ATGGGGTTTT TAGTGGTGGT 
AGCCCGTTGC AGGCAACCAG TTCTCCTCAG GGTGTGGTGC AAGCTGTTGT TCTGCCAACA 
GTGGGTCTGG TGTCTCCCAT AAGCATCAAC TTAAGTGACA TTCAAAATGT ACTTAAAGTG 
GCAGTGGATG GTAATGTAAT AAGGCAAGTA TTGGAAAACA ATCACGCTAA TCTTGCATCC 
AAAGAACAAG AAACAATCAG CAATGCATCT ATACAACAAG CTGGCCATTC CCTCATTTCA 
GCTATCAGTC TTCCTTTGGT TGACCAAGAT GGGACAACCA AAATTATCAT CAACTACAGC 
TTGGAGCAGC CAAGTCAACT TCAGGTTGTT CCCCAAAATC TAAAAAAAGA ACATTCTGTT 
CCTACAAACA GTTGCAAAAA TGAAAAGTTA CCAGAAGATC TTACGGTGAA GTCTGAGAAA 
GATAAAAACT TTGAAGGAGA GACCAACGAT AGCACTTGTC TTCTTTGTGA TGACTGTCCA 
GGAGATCTTA ATGCACTTCA AGAATTAAAG CACTATGAAA CAAAAAATCC TCCTCAGCTT 
CCCCAGTCCA GTGGAACAGA AGCTGAGAAG CCCAGCTCCC CTGCCCCATC AGAAACTGGG 
GAGAACAACT TATCTCCTGG TCAGCCACCC TTAAAGAACC TTTTATCGCT CCTAAAAGCA 
TATTATGCAT TAAATGCACA ACCAAGCGCA GAAGAGCTTT CAAAAATTGC CGATTCCGTG 
AACCTACCAC TGGATGTGGT AAAAAAGTGG TTTGAAAAAA TGCAAGCTGG ACAAATTTCT 
GTGCAGTCTT CTGGACCATC TTCTCCTGAA CAAGTTAAAA TAAGCAGTCC CACAGATAAC 
GATGATCAAG CAGCAACTAC AAATGAGAGT GAACCCCAGA ACAGCACAAA CAACTCACAA 
AATCCGGCCA ATACAAGTAA ATCTCAGACT TCATCAGGGG GATCAACTCA GAATGGTTCG 
CGCAGTAGCA CGCCATCCCC ATCACCACTA AACCTTTCTT CATCAAGAAA TTCACAGGGT 
TATACGTACA CAGCAGAGGG TGTACAAGAA GAGCCACAAA TGGAACCTCT TGACCTTTCA 
CTACCAAAGC AACATGGAGA ACTGTTGGAA AGATCTACCA TAACTAGTGT TTACCAGAAC 
AGTGTTTATT CTGTCCAGGA AGAACCTTTG AACTTAACTT GTGCAAAAAA AGAACCACAA 
AAGGACAACA GTATTACAGA CTCTGATCCT ATTGTAAATG TAATCCCACC AAGTGCCAAT 
CCCATAAATA TTGCTATACC TACAGTCACT GCCCAGTTAC CTACAATTGT TGCCATTGCT 
GACCAGAACA GTGTTCCATG CTTGAGAGCT CTTGCTGCCA ATAAGCAAAC CATTTTGATT 
CCCCAGGTGG CTTATACGTA CTCTACTACA GTTAGTCCTG CAGTTCAGGA AACACCACCA 
AAACAGACCC AAGCCAACGG AAGTCAGGAC GAAAGGCAGG ACACTAGCTC AGAAGGAGTA 
TCGAATGTAG AAGATCAAAA TGATTCTGAT TCAACACCCC CAAAGAAAAA GATGAGAAAG 
ACAGAAAATG GGATGTATGC ATGTGATTTG TGTGACAAAA TATTCCAGAA GAGCAGCTCA 
TTATTGAGAC ATAAGTATGA ACACACAGGT AAAAGACCTC ATGAGTGTGG AATCTGTAAA 
AAAGCCTTCA AACACAAACA CCATTTGATC GAACACATGC GACTGCATTC TGGGGAGAAA 
CCCTACCAAT GTGACAAATG CGGGAAGCGC TTCTCACACT CGGGGTCGTA CTCTCAGCAC 
ATGAATCATC GCTACTCCTA TTGCAAAAGA GAGGCAGAGG AGCGTGACAG CACAGAGCAG 
GAGGAGGTGG GACAGGAGGT GCTCAGCAGC GAGCATGCTG GTGCCAGGGC ATCGCCATCG 
CAGATCGACT CCGATGAGAG AGAGAGTCTA ACCAGGGAAG AAGAGGAAGA TAGTGAAAAA 
GAGGAAGAGG AGGAGGAAGA AAAGGACGTA GAAGGACTTC AGGAAGAAAA AGAATGTAGA 
AAACTACAAG ATGTAGAGGA GGAAGAAGAA GTAGAAGAAG AAGAAGAAGA AGAGGAAGGG 
AAAACTGAAG GTAACAAGAA CGATGATGTT GTAAATCGAG CAAGCAATGC AGAACCAGAA 
GTTATACAGA GCAATGGGCA GGTGTCAGAA GAAAAAACAA ATAAAGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025002824.1
CDS	730..3420
Translation	MTSHKSGRDQRHVTQSSGNRKFKCTECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCIGLMPVNGRARSGLKMSQCSSPSLSASPGSPARPQIRQKIENKPLQEQLPVNQIKTEPVDYEFKPIVVASGINCSTPLQNGVFSGGSPLQATSSPQGVVQAVVLPTVGLVSPISINLSDIQNVLKVAVDGNVIRQVLENNHANLASKEQETISNASIQQAGHSLISAISLPLVDQDGTTKIIINYSLEQPSQLQVVPQNLKKEHSVPTNSCKNEKLPEDLTVKSEKDKNFEGETNDSTCLLCDDCPGDLNALQELKHYETKNPPQLPQSSGTEAEKPSSPAPSETGENNLSPGQPPLKNLLSLLKAYYALNAQPSAEELSKIADSVNLPLDVVKKWFEKMQAGQISVQSSGPSSPEQVKISSPTDNDDQAATTNESEPQNSTNNSQNPANTSKSQTSSGGSTQNGSRSSTPSPSPLNLSSSRNSQGYTYTAEGVQEEPQMEPLDLSLPKQHGELLERSTITSVYQNSVYSVQEEPLNLTCAKKEPQKDNSITDSDPIVNVIPPSANPINIAIPTVTAQLPTIVAIADQNSVPCLRALAANKQTILIPQVAYTYSTTVSPAVQETPPKQTQANGSQDERQDTSSEGVSNVEDQNDSDSTPPKKKMRKTENGMYACDLCDKIFQKSSSLLRHKYEHTGKRPHECGICKKAFKHKHHLIEHMRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKREAEERDSTEQEEVGQEVLSSEHAGARASPSQIDSDERESLTREEEEDSEKEEEEEEEKDVEGLQEEKECRKLQDVEEEEEVEEEEEEEEGKTEGNKNDDVVNRASNAEPEVIQSNGQVSEEKTNKA

Target ORF information:

RefSeq Version	XM_025147056.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus zinc finger E-box binding homeobox 1 (ZEB1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025147056.1

ORF Insert Sequence:

ATGACGTCGC ACAAGTCGGG ACGAGATCAG AGACATGTGA CGCAGTCCAG TGGTAATCGA 
AAATTCAAGT GCACTGAATG TGGAAAAGCT TTCAAATATA AACATCATCT AAAGGAACAC 
CTACGAATCC ACAGTGGAGA GAAGCCATAT GAGTGCCCAA ACTGCAAGAA ACGTTTTTCC 
CATTCTGGTT CATACAGCTC ACACATAAGC AGTAAGAAGT GTATTGGTTT GATGCCCGTG 
AATGGTCGAG CTCGGTCAGG GCTCAAGATG TCTCAGTGCT CCTCCCCTTC CCTTTCTGCA 
TCGCCCGGTA GCCCAGCAAG ACCACAGATA CGACAAAAGA TAGAAAATAA ACCCTTGCAA 
GAGCAACTTC CTGTTAACCA AATTAAAACT GAACCTGTGG ATTATGAATT CAAGCCCATA 
GTGGTTGCTT CAGGAATTAA TTGTTCGACC CCTTTGCAGA ATGGGGTTTT TAGTGGTGGT 
AGCCCGTTGC AGGCAACCAG TTCTCCTCAG GGTGTGGTGC AAGCTGTTGT TCTGCCAACA 
GTGGGTCTGG TGTCTCCCAT AAGCATCAAC TTAAGTGACA TTCAAAATGT ACTTAAAGTG 
GCAGTGGATG GTAATGTAAT AAGGCAAGTA TTGGAAAACA ATCACGCTAA TCTTGCATCC 
AAAGAACAAG AAACAATCAG CAATGCATCT ATACAACAAG CTGGCCATTC CCTCATTTCA 
GCTATCAGTC TTCCTTTGGT TGACCAAGAT GGGACAACCA AAATTATCAT CAACTACAGC 
TTGGAGCAGC CAAGTCAACT TCAGGTTGTT CCCCAAAATC TAAAAAAAGA ACATTCTGTT 
CCTACAAACA GTTGCAAAAA TGAAAAGTTA CCAGAAGATC TTACGGTGAA GTCTGAGAAA 
GATAAAAACT TTGAAGGAGA GACCAACGAT AGCACTTGTC TTCTTTGTGA TGACTGTCCA 
GGAGATCTTA ATGCACTTCA AGAATTAAAG CACTATGAAA CAAAAAATCC TCCTCAGCTT 
CCCCAGTCCA GTGGAACAGA AGCTGAGAAG CCCAGCTCCC CTGCCCCATC AGAAACTGGG 
GAGAACAACT TATCTCCTGG TCAGCCACCC TTAAAGAACC TTTTATCGCT CCTAAAAGCA 
TATTATGCAT TAAATGCACA ACCAAGCGCA GAAGAGCTTT CAAAAATTGC CGATTCCGTG 
AACCTACCAC TGGATGTGGT AAAAAAGTGG TTTGAAAAAA TGCAAGCTGG ACAAATTTCT 
GTGCAGTCTT CTGGACCATC TTCTCCTGAA CAAGTTAAAA TAAGCAGTCC CACAGATAAC 
GATGATCAAG CAGCAACTAC AAATGAGAGT GAACCCCAGA ACAGCACAAA CAACTCACAA 
AATCCGGCCA ATACAAGTAA ATCTCAGACT TCATCAGGGG GATCAACTCA GAATGGTTCG 
CGCAGTAGCA CGCCATCCCC ATCACCACTA AACCTTTCTT CATCAAGAAA TTCACAGGGT 
TATACGTACA CAGCAGAGGG TGTACAAGAA GAGCCACAAA TGGAACCTCT TGACCTTTCA 
CTACCAAAGC AACATGGAGA ACTGTTGGAA AGATCTACCA TAACTAGTGT TTACCAGAAC 
AGTGTTTATT CTGTCCAGGA AGAACCTTTG AACTTAACTT GTGCAAAAAA AGAACCACAA 
AAGGACAACA GTATTACAGA CTCTGATCCT ATTGTAAATG TAATCCCACC AAGTGCCAAT 
CCCATAAATA TTGCTATACC TACAGTCACT GCCCAGTTAC CTACAATTGT TGCCATTGCT 
GACCAGAACA GTGTTCCATG CTTGAGAGCT CTTGCTGCCA ATAAGCAAAC CATTTTGATT 
CCCCAGGTGG CTTATACGTA CTCTACTACA GTTAGTCCTG CAGTTCAGGA AACACCACCA 
AAACAGACCC AAGCCAACGG AAGTCAGGAC GAAAGGCAGG ACACTAGCTC AGAAGGAGTA 
TCGAATGTAG AAGATCAAAA TGATTCTGAT TCAACACCCC CAAAGAAAAA GATGAGAAAG 
ACAGAAAATG GGATGTATGC ATGTGATTTG TGTGACAAAA TATTCCAGAA GAGCAGCTCA 
TTATTGAGAC ATAAGTATGA ACACACAGGT AAAAGACCTC ATGAGTGTGG AATCTGTAAA 
AAAGCCTTCA AACACAAACA CCATTTGATC GAACACATGC GACTGCATTC TGGGGAGAAA 
CCCTACCAAT GTGACAAATG CGGGAAGCGC TTCTCACACT CGGGGTCGTA CTCTCAGCAC 
ATGAATCATC GCTACTCCTA TTGCAAAAGA GAGGCAGAGG AGCGTGACAG CACAGAGCAG 
GAGGAGGTGG GACAGGAGGT GCTCAGCAGC GAGCATGCTG GTGCCAGGGC ATCGCCATCG 
CAGATCGACT CCGATGAGAG AGAGAGTCTA ACCAGGGAAG AAGAGGAAGA TAGTGAAAAA 
GAGGAAGAGG AGGAGGAAGA AAAGGACGTA GAAGGACTTC AGGAAGAAAA AGAATGTAGA 
AAACTACAAG ATGTAGAGGA GGAAGAAGAA GTAGAAGAAG AAGAAGAAGA AGAGGAAGGG 
AAAACTGAAG GTAACAAGAA CGATGATGTT GTAAATCGAG CAAGCAATGC AGAACCAGAA 
GTTATACAGA GCAATGGGCA GGTGTCAGAA GAAAAAACAA ATAAAGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZEB1 ( XM_025147055.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025002823.1 Gallus gallus zinc finger E-box binding homeobox 1 (ZEB1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa145753
Clone ID Related Accession (Same CDS sequence)	XM_025147055.1
Accession Version	XM_025147055.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	zinc finger E-box-binding homeobox 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTT GTGCAGTCAC CAATTACAAT AACGTGATAG AAGCAAACTC AGATTCAGAT 
GACGAAGATA AATTGCATAT AGTGGAAGAA GAAAGTATAA CTGATGCAGC AGACTGCGAT 
GCAAGTGTGC CAGAAGATGA CTTGCCAACA GACCACACCG TGTTACCAGA AAACAGTGAG 
AGGGAAGGGA GCACAAACAG CTGCTGGGAA GATGAAGCAG GAAAAGAAAC AAAGGAAATC 
CTGGGGCCTG AAGCTCAGTC AGATGAAGTT GGATGTACAG TAAAAGAAGA TGAATGTGAT 
TCTGATGCAG AAAATGAACA GAACCATGAC CCTAATGTTG AAGAATTCCT TCAACAAGAA 
GATACAGCTG TTATTTACCC TGAAGCACCT GAGGAGGACC AGAGACAAGG CACACCAGAA 
GCTAGTGGTC AGGATGAAAA TGGAACGCCC GATGCATTTT CCCAGCTGCT CACTTGCCCG 
TACTGTGACA GAGGGTACAA ACGCTTCACC TCTCTGAAGG AACACATTAA ATACCGCCAT 
GAAAAGAACG AGGATAACTT CAGTTGCTCC CTCTGCAGTT ACACGTTTGC GTATAGAACA 
CAGCTGGACC GCCACATGAC GTCGCACAAG TCGGGACGAG ATCAGAGACA TGTGACGCAG 
TCCAGTGGTA ATCGAAAATT CAAGTGCACT GAATGTGGAA AAGCTTTCAA ATATAAACAT 
CATCTAAAGG AACACCTACG AATCCACAGT GGAGAGAAGC CATATGAGTG CCCAAACTGC 
AAGAAACGTT TTTCCCATTC TGGTTCATAC AGCTCACACA TAAGCAGTAA GAAGTGTATT 
GGTTTGATGC CCGTGAATGG TCGAGCTCGG TCAGGGCTCA AGATGTCTCA GTGCTCCTCC 
CCTTCCCTTT CTGCATCGCC CGGTAGCCCA GCAAGACCAC AGATACGACA AAAGATAGAA 
AATAAACCCT TGCAAGAGCA ACTTCCTGTT AACCAAATTA AAACTGAACC TGTGGATTAT 
GAATTCAAGC CCATAGTGGT TGCTTCAGGA ATTAATTGTT CGACCCCTTT GCAGAATGGG 
GTTTTTAGTG GTGGTAGCCC GTTGCAGGCA ACCAGTTCTC CTCAGGGTGT GGTGCAAGCT 
GTTGTTCTGC CAACAGTGGG TCTGGTGTCT CCCATAAGCA TCAACTTAAG TGACATTCAA 
AATGTACTTA AAGTGGCAGT GGATGGTAAT GTAATAAGGC AAGTATTGGA AAACAATCAC 
GCTAATCTTG CATCCAAAGA ACAAGAAACA ATCAGCAATG CATCTATACA ACAAGCTGGC 
CATTCCCTCA TTTCAGCTAT CAGTCTTCCT TTGGTTGACC AAGATGGGAC AACCAAAATT 
ATCATCAACT ACAGCTTGGA GCAGCCAAGT CAACTTCAGG TTGTTCCCCA AAATCTAAAA 
AAAGAACATT CTGTTCCTAC AAACAGTTGC AAAAATGAAA AGTTACCAGA AGATCTTACG 
GTGAAGTCTG AGAAAGATAA AAACTTTGAA GGAGAGACCA ACGATAGCAC TTGTCTTCTT 
TGTGATGACT GTCCAGGAGA TCTTAATGCA CTTCAAGAAT TAAAGCACTA TGAAACAAAA 
AATCCTCCTC AGCTTCCCCA GTCCAGTGGA ACAGAAGCTG AGAAGCCCAG CTCCCCTGCC 
CCATCAGAAA CTGGGGAGAA CAACTTATCT CCTGGTCAGC CACCCTTAAA GAACCTTTTA 
TCGCTCCTAA AAGCATATTA TGCATTAAAT GCACAACCAA GCGCAGAAGA GCTTTCAAAA 
ATTGCCGATT CCGTGAACCT ACCACTGGAT GTGGTAAAAA AGTGGTTTGA AAAAATGCAA 
GCTGGACAAA TTTCTGTGCA GTCTTCTGGA CCATCTTCTC CTGAACAAGT TAAAATAAGC 
AGTCCCACAG ATAACGATGA TCAAGCAGCA ACTACAAATG AGAGTGAACC CCAGAACAGC 
ACAAACAACT CACAAAATCC GGCCAATACA AGTAAATCTC AGACTTCATC AGGGGGATCA 
ACTCAGAATG GTTCGCGCAG TAGCACGCCA TCCCCATCAC CACTAAACCT TTCTTCATCA 
AGAAATTCAC AGGGTTATAC GTACACAGCA GAGGGTGTAC AAGAAGAGCC ACAAATGGAA 
CCTCTTGACC TTTCACTACC AAAGCAACAT GGAGAACTGT TGGAAAGATC TACCATAACT 
AGTGTTTACC AGAACAGTGT TTATTCTGTC CAGGAAGAAC CTTTGAACTT AACTTGTGCA 
AAAAAAGAAC CACAAAAGGA CAACAGTATT ACAGACTCTG ATCCTATTGT AAATGTAATC 
CCACCAAGTG CCAATCCCAT AAATATTGCT ATACCTACAG TCACTGCCCA GTTACCTACA 
ATTGTTGCCA TTGCTGACCA GAACAGTGTT CCATGCTTGA GAGCTCTTGC TGCCAATAAG 
CAAACCATTT TGATTCCCCA GGTGGCTTAT ACGTACTCTA CTACAGTTAG TCCTGCAGTT 
CAGGAAACAC CACCAAAACA GACCCAAGCC AACGGAAGTC AGGACGAAAG GCAGGACACT 
AGCTCAGAAG GAGTATCGAA TGTAGAAGAT CAAAATGATT CTGATTCAAC ACCCCCAAAG 
AAAAAGATGA GAAAGACAGA AAATGGGATG TATGCATGTG ATTTGTGTGA CAAAATATTC 
CAGAAGAGCA GCTCATTATT GAGACATAAG TATGAACACA CAGGTAAAAG ACCTCATGAG 
TGTGGAATCT GTAAAAAAGC CTTCAAACAC AAACACCATT TGATCGAACA CATGCGACTG 
CATTCTGGGG AGAAACCCTA CCAATGTGAC AAATGCGGGA AGCGCTTCTC ACACTCGGGG 
TCGTACTCTC AGCACATGAA TCATCGCTAC TCCTATTGCA AAAGAGAGGC AGAGGAGCGT 
GACAGCACAG AGCAGGAGGA GGTGGGACAG GAGGTGCTCA GCAGCGAGCA TGCTGGTGCC 
AGGGCATCGC CATCGCAGAT CGACTCCGAT GAGAGAGAGA GTCTAACCAG GGAAGAAGAG 
GAAGATAGTG AAAAAGAGGA AGAGGAGGAG GAAGAAAAGG ACGTAGAAGG ACTTCAGGAA 
GAAAAAGAAT GTAGAAAACT ACAAGATGTA GAGGAGGAAG AAGAAGTAGA AGAAGAAGAA 
GAAGAAGAGG AAGGGAAAAC TGAAGGTAAC AAGAACGATG ATGTTGTAAA TCGAGCAAGC 
AATGCAGAAC CAGAAGTTAT ACAGAGCAAT GGGCAGGTGT CAGAAGAAAA AACAAATAAA 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025002823.1
CDS	205..3510
Translation	MATCAVTNYNNVIEANSDSDDEDKLHIVEEESITDAADCDASVPEDDLPTDHTVLPENSEREGSTNSCWEDEAGKETKEILGPEAQSDEVGCTVKEDECDSDAENEQNHDPNVEEFLQQEDTAVIYPEAPEEDQRQGTPEASGQDENGTPDAFSQLLTCPYCDRGYKRFTSLKEHIKYRHEKNEDNFSCSLCSYTFAYRTQLDRHMTSHKSGRDQRHVTQSSGNRKFKCTECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCIGLMPVNGRARSGLKMSQCSSPSLSASPGSPARPQIRQKIENKPLQEQLPVNQIKTEPVDYEFKPIVVASGINCSTPLQNGVFSGGSPLQATSSPQGVVQAVVLPTVGLVSPISINLSDIQNVLKVAVDGNVIRQVLENNHANLASKEQETISNASIQQAGHSLISAISLPLVDQDGTTKIIINYSLEQPSQLQVVPQNLKKEHSVPTNSCKNEKLPEDLTVKSEKDKNFEGETNDSTCLLCDDCPGDLNALQELKHYETKNPPQLPQSSGTEAEKPSSPAPSETGENNLSPGQPPLKNLLSLLKAYYALNAQPSAEELSKIADSVNLPLDVVKKWFEKMQAGQISVQSSGPSSPEQVKISSPTDNDDQAATTNESEPQNSTNNSQNPANTSKSQTSSGGSTQNGSRSSTPSPSPLNLSSSRNSQGYTYTAEGVQEEPQMEPLDLSLPKQHGELLERSTITSVYQNSVYSVQEEPLNLTCAKKEPQKDNSITDSDPIVNVIPPSANPINIAIPTVTAQLPTIVAIADQNSVPCLRALAANKQTILIPQVAYTYSTTVSPAVQETPPKQTQANGSQDERQDTSSEGVSNVEDQNDSDSTPPKKKMRKTENGMYACDLCDKIFQKSSSLLRHKYEHTGKRPHECGICKKAFKHKHHLIEHMRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKREAEERDSTEQEEVGQEVLSSEHAGARASPSQIDSDERESLTREEEEDSEKEEEEEEEKDVEGLQEEKECRKLQDVEEEEEVEEEEEEEEGKTEGNKNDDVVNRASNAEPEVIQSNGQVSEEKTNKA

Target ORF information:

RefSeq Version	XM_025147055.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus zinc finger E-box binding homeobox 1 (ZEB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025147055.1

ORF Insert Sequence:

ATGGCAACTT GTGCAGTCAC CAATTACAAT AACGTGATAG AAGCAAACTC AGATTCAGAT 
GACGAAGATA AATTGCATAT AGTGGAAGAA GAAAGTATAA CTGATGCAGC AGACTGCGAT 
GCAAGTGTGC CAGAAGATGA CTTGCCAACA GACCACACCG TGTTACCAGA AAACAGTGAG 
AGGGAAGGGA GCACAAACAG CTGCTGGGAA GATGAAGCAG GAAAAGAAAC AAAGGAAATC 
CTGGGGCCTG AAGCTCAGTC AGATGAAGTT GGATGTACAG TAAAAGAAGA TGAATGTGAT 
TCTGATGCAG AAAATGAACA GAACCATGAC CCTAATGTTG AAGAATTCCT TCAACAAGAA 
GATACAGCTG TTATTTACCC TGAAGCACCT GAGGAGGACC AGAGACAAGG CACACCAGAA 
GCTAGTGGTC AGGATGAAAA TGGAACGCCC GATGCATTTT CCCAGCTGCT CACTTGCCCG 
TACTGTGACA GAGGGTACAA ACGCTTCACC TCTCTGAAGG AACACATTAA ATACCGCCAT 
GAAAAGAACG AGGATAACTT CAGTTGCTCC CTCTGCAGTT ACACGTTTGC GTATAGAACA 
CAGCTGGACC GCCACATGAC GTCGCACAAG TCGGGACGAG ATCAGAGACA TGTGACGCAG 
TCCAGTGGTA ATCGAAAATT CAAGTGCACT GAATGTGGAA AAGCTTTCAA ATATAAACAT 
CATCTAAAGG AACACCTACG AATCCACAGT GGAGAGAAGC CATATGAGTG CCCAAACTGC 
AAGAAACGTT TTTCCCATTC TGGTTCATAC AGCTCACACA TAAGCAGTAA GAAGTGTATT 
GGTTTGATGC CCGTGAATGG TCGAGCTCGG TCAGGGCTCA AGATGTCTCA GTGCTCCTCC 
CCTTCCCTTT CTGCATCGCC CGGTAGCCCA GCAAGACCAC AGATACGACA AAAGATAGAA 
AATAAACCCT TGCAAGAGCA ACTTCCTGTT AACCAAATTA AAACTGAACC TGTGGATTAT 
GAATTCAAGC CCATAGTGGT TGCTTCAGGA ATTAATTGTT CGACCCCTTT GCAGAATGGG 
GTTTTTAGTG GTGGTAGCCC GTTGCAGGCA ACCAGTTCTC CTCAGGGTGT GGTGCAAGCT 
GTTGTTCTGC CAACAGTGGG TCTGGTGTCT CCCATAAGCA TCAACTTAAG TGACATTCAA 
AATGTACTTA AAGTGGCAGT GGATGGTAAT GTAATAAGGC AAGTATTGGA AAACAATCAC 
GCTAATCTTG CATCCAAAGA ACAAGAAACA ATCAGCAATG CATCTATACA ACAAGCTGGC 
CATTCCCTCA TTTCAGCTAT CAGTCTTCCT TTGGTTGACC AAGATGGGAC AACCAAAATT 
ATCATCAACT ACAGCTTGGA GCAGCCAAGT CAACTTCAGG TTGTTCCCCA AAATCTAAAA 
AAAGAACATT CTGTTCCTAC AAACAGTTGC AAAAATGAAA AGTTACCAGA AGATCTTACG 
GTGAAGTCTG AGAAAGATAA AAACTTTGAA GGAGAGACCA ACGATAGCAC TTGTCTTCTT 
TGTGATGACT GTCCAGGAGA TCTTAATGCA CTTCAAGAAT TAAAGCACTA TGAAACAAAA 
AATCCTCCTC AGCTTCCCCA GTCCAGTGGA ACAGAAGCTG AGAAGCCCAG CTCCCCTGCC 
CCATCAGAAA CTGGGGAGAA CAACTTATCT CCTGGTCAGC CACCCTTAAA GAACCTTTTA 
TCGCTCCTAA AAGCATATTA TGCATTAAAT GCACAACCAA GCGCAGAAGA GCTTTCAAAA 
ATTGCCGATT CCGTGAACCT ACCACTGGAT GTGGTAAAAA AGTGGTTTGA AAAAATGCAA 
GCTGGACAAA TTTCTGTGCA GTCTTCTGGA CCATCTTCTC CTGAACAAGT TAAAATAAGC 
AGTCCCACAG ATAACGATGA TCAAGCAGCA ACTACAAATG AGAGTGAACC CCAGAACAGC 
ACAAACAACT CACAAAATCC GGCCAATACA AGTAAATCTC AGACTTCATC AGGGGGATCA 
ACTCAGAATG GTTCGCGCAG TAGCACGCCA TCCCCATCAC CACTAAACCT TTCTTCATCA 
AGAAATTCAC AGGGTTATAC GTACACAGCA GAGGGTGTAC AAGAAGAGCC ACAAATGGAA 
CCTCTTGACC TTTCACTACC AAAGCAACAT GGAGAACTGT TGGAAAGATC TACCATAACT 
AGTGTTTACC AGAACAGTGT TTATTCTGTC CAGGAAGAAC CTTTGAACTT AACTTGTGCA 
AAAAAAGAAC CACAAAAGGA CAACAGTATT ACAGACTCTG ATCCTATTGT AAATGTAATC 
CCACCAAGTG CCAATCCCAT AAATATTGCT ATACCTACAG TCACTGCCCA GTTACCTACA 
ATTGTTGCCA TTGCTGACCA GAACAGTGTT CCATGCTTGA GAGCTCTTGC TGCCAATAAG 
CAAACCATTT TGATTCCCCA GGTGGCTTAT ACGTACTCTA CTACAGTTAG TCCTGCAGTT 
CAGGAAACAC CACCAAAACA GACCCAAGCC AACGGAAGTC AGGACGAAAG GCAGGACACT 
AGCTCAGAAG GAGTATCGAA TGTAGAAGAT CAAAATGATT CTGATTCAAC ACCCCCAAAG 
AAAAAGATGA GAAAGACAGA AAATGGGATG TATGCATGTG ATTTGTGTGA CAAAATATTC 
CAGAAGAGCA GCTCATTATT GAGACATAAG TATGAACACA CAGGTAAAAG ACCTCATGAG 
TGTGGAATCT GTAAAAAAGC CTTCAAACAC AAACACCATT TGATCGAACA CATGCGACTG 
CATTCTGGGG AGAAACCCTA CCAATGTGAC AAATGCGGGA AGCGCTTCTC ACACTCGGGG 
TCGTACTCTC AGCACATGAA TCATCGCTAC TCCTATTGCA AAAGAGAGGC AGAGGAGCGT 
GACAGCACAG AGCAGGAGGA GGTGGGACAG GAGGTGCTCA GCAGCGAGCA TGCTGGTGCC 
AGGGCATCGC CATCGCAGAT CGACTCCGAT GAGAGAGAGA GTCTAACCAG GGAAGAAGAG 
GAAGATAGTG AAAAAGAGGA AGAGGAGGAG GAAGAAAAGG ACGTAGAAGG ACTTCAGGAA 
GAAAAAGAAT GTAGAAAACT ACAAGATGTA GAGGAGGAAG AAGAAGTAGA AGAAGAAGAA 
GAAGAAGAGG AAGGGAAAAC TGAAGGTAAC AAGAACGATG ATGTTGTAAA TCGAGCAAGC 
AATGCAGAAC CAGAAGTTAT ACAGAGCAAT GGGCAGGTGT CAGAAGAAAA AACAAATAAA 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZEB1 ( XM_015281304.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015136790.1 Gallus gallus zinc finger E-box binding homeobox 1 (ZEB1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa31494
Clone ID Related Accession (Same CDS sequence)	XM_015281304.1 , XM_015281304.2
Accession Version	XM_015281304.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	zinc finger E-box-binding homeobox 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG GCCCCAGGTG TAAGCGCAGG AAGCAGGCGA ACCCGAGGCG CAACAACGTC 
ACCAATTACA ATAACGTGAT AGAAGCAAAC TCAGATTCAG ATGACGAAGA TAAATTGCAT 
ATAGTGGAAG AAGAAAGTAT AACTGATGCA GCAGACTGCG ATGCAAGTGT GCCAGAAGAT 
GACTTGCCAA CAGACCACAC CGTGTTACCA GAAAACAGTG AGAGGGAAGG GAGCACAAAC 
AGCTGCTGGG AAGATGAAGC AGGAAAAGAA ACAAAGGAAA TCCTGGGGCC TGAAGCTCAG 
TCAGATGAAG TTGGATGTAC AGTAAAAGAA GATGAATGTG ATTCTGATGC AGAAAATGAA 
CAGAACCATG ACCCTAATGT TGAAGAATTC CTTCAACAAG AAGATACAGC TGTTATTTAC 
CCTGAAGCAC CTGAGGAGGA CCAGAGACAA GGCACACCAG AAGCTAGTGG TCAGGATGAA 
AATGGAACGC CCGATGCATT TTCCCAGCTG CTCACTTGCC CGTACTGTGA CAGAGGGTAC 
AAACGCTTCA CCTCTCTGAA GGAACACATT AAATACCGCC ATGAAAAGAA CGAGGATAAC 
TTCAGTTGCT CCCTCTGCAG TTACACGTTT GCGTATAGAA CACAGCTGGA CCGCCACATG 
ACGTCGCACA AGTCGGGACG AGATCAGAGA CATGTGACGC AGTCCAGTGG TAATCGAAAA 
TTCAAGTGCA CTGAATGTGG AAAAGCTTTC AAATATAAAC ATCATCTAAA GGAACACCTA 
CGAATCCACA GTGGAGAGAA GCCATATGAG TGCCCAAACT GCAAGAAACG TTTTTCCCAT 
TCTGGTTCAT ACAGCTCACA CATAAGCAGT AAGAAGTGTA TTGGTTTGAT GCCCGTGAAT 
GGTCGAGCTC GGTCAGGGCT CAAGATGTCT CAGTGCTCCT CCCCTTCCCT TTCTGCATCG 
CCCGGTAGCC CAGCAAGACC ACAGATACGA CAAAAGATAG AAAATAAACC CTTGCAAGAG 
CAACTTCCTG TTAACCAAAT TAAAACTGAA CCTGTGGATT ATGAATTCAA GCCCATAGTG 
GTTGCTTCAG GAATTAATTG TTCGACCCCT TTGCAGAATG GGGTTTTTAG TGGTGGTAGC 
CCGTTGCAGG CAACCAGTTC TCCTCAGGGT GTGGTGCAAG CTGTTGTTCT GCCAACAGTG 
GGTCTGGTGT CTCCCATAAG CATCAACTTA AGTGACATTC AAAATGTACT TAAAGTGGCA 
GTGGATGGTA ATGTAATAAG GCAAGTATTG GAAAACAATC ACGCTAATCT TGCATCCAAA 
GAACAAGAAA CAATCAGCAA TGCATCTATA CAACAAGCTG GCCATTCCCT CATTTCAGCT 
ATCAGTCTTC CTTTGGTTGA CCAAGATGGG ACAACCAAAA TTATCATCAA CTACAGCTTG 
GAGCAGCCAA GTCAACTTCA GGTTGTTCCC CAAAATCTAA AAAAAGAACA TTCTGTTCCT 
ACAAACAGTT GCAAAAATGA AAAGTTACCA GAAGATCTTA CGGTGAAGTC TGAGAAAGAT 
AAAAACTTTG AAGGAGAGAC CAACGATAGC ACTTGTCTTC TTTGTGATGA CTGTCCAGGA 
GATCTTAATG CACTTCAAGA ATTAAAGCAC TATGAAACAA AAAATCCTCC TCAGCTTCCC 
CAGTCCAGTG GAACAGAAGC TGAGAAGCCC AGCTCCCCTG CCCCATCAGA AACTGGGGAG 
AACAACTTAT CTCCTGGTCA GCCACCCTTA AAGAACCTTT TATCGCTCCT AAAAGCATAT 
TATGCATTAA ATGCACAACC AAGCGCAGAA GAGCTTTCAA AAATTGCCGA TTCCGTGAAC 
CTACCACTGG ATGTGGTAAA AAAGTGGTTT GAAAAAATGC AAGCTGGACA AATTTCTGTG 
CAGTCTTCTG GACCATCTTC TCCTGAACAA GTTAAAATAA GCAGTCCCAC AGATAACGAT 
GATCAAGCAG CAACTACAAA TGAGAGTGAA CCCCAGAACA GCACAAACAA CTCACAAAAT 
CCGGCCAATA CAAGTAAATC TCAGACTTCA TCAGGGGGAT CAACTCAGAA TGGTTCGCGC 
AGTAGCACGC CATCCCCATC ACCACTAAAC CTTTCTTCAT CAAGAAATTC ACAGGGTTAT 
ACGTACACAG CAGAGGGTGT ACAAGAAGAG CCACAAATGG AACCTCTTGA CCTTTCACTA 
CCAAAGCAAC ATGGAGAACT GTTGGAAAGA TCTACCATAA CTAGTGTTTA CCAGAACAGT 
GTTTATTCTG TCCAGGAAGA ACCTTTGAAC TTAACTTGTG CAAAAAAAGA ACCACAAAAG 
GACAACAGTA TTACAGACTC TGATCCTATT GTAAATGTAA TCCCACCAAG TGCCAATCCC 
ATAAATATTG CTATACCTAC AGTCACTGCC CAGTTACCTA CAATTGTTGC CATTGCTGAC 
CAGAACAGTG TTCCATGCTT GAGAGCTCTT GCTGCCAATA AGCAAACCAT TTTGATTCCC 
CAGGTGGCTT ATACGTACTC TACTACAGTT AGTCCTGCAG TTCAGGAAAC ACCACCAAAA 
CAGACCCAAG CCAACGGAAG TCAGGACGAA AGGCAGGACA CTAGCTCAGA AGGAGTATCG 
AATGTAGAAG ATCAAAATGA TTCTGATTCA ACACCCCCAA AGAAAAAGAT GAGAAAGACA 
GAAAATGGGA TGTATGCATG TGATTTGTGT GACAAAATAT TCCAGAAGAG CAGCTCATTA 
TTGAGACATA AGTATGAACA CACAGGTAAA AGACCTCATG AGTGTGGAAT CTGTAAAAAA 
GCCTTCAAAC ACAAACACCA TTTGATCGAA CACATGCGAC TGCATTCTGG GGAGAAACCC 
TACCAATGTG ACAAATGCGG GAAGCGCTTC TCACACTCGG GGTCGTACTC TCAGCACATG 
AATCATCGCT ACTCCTATTG CAAAAGAGAG GCAGAGGAGC GTGACAGCAC AGAGCAGGAG 
GAGGTGGGAC AGGAGGTGCT CAGCAGCGAG CATGCTGGTG CCAGGGCATC GCCATCGCAG 
ATCGACTCCG ATGAGAGAGA GAGTCTAACC AGGGAAGAAG AGGAAGATAG TGAAAAAGAG 
GAAGAGGAGG AGGAAGAAAA GGACGTAGAA GGACTTCAGG AAGAAAAAGA ATGTAGAAAA 
CTACAAGATG TAGAGGAGGA AGAAGAAGTA GAAGAAGAAG AAGAAGAAGA GGAAGGGAAA 
ACTGAAGGTA ACAAGAACGA TGATGTTGTA AATCGAGCAA GCAATGCAGA ACCAGAAGTT 
ATACAGAGCA ATGGGCAGGT GTCAGAAGAA AAAACAAATA AAGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015136790.1
CDS	49..3396
Translation	MADGPRCKRRKQANPRRNNVTNYNNVIEANSDSDDEDKLHIVEEESITDAADCDASVPEDDLPTDHTVLPENSEREGSTNSCWEDEAGKETKEILGPEAQSDEVGCTVKEDECDSDAENEQNHDPNVEEFLQQEDTAVIYPEAPEEDQRQGTPEASGQDENGTPDAFSQLLTCPYCDRGYKRFTSLKEHIKYRHEKNEDNFSCSLCSYTFAYRTQLDRHMTSHKSGRDQRHVTQSSGNRKFKCTECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCIGLMPVNGRARSGLKMSQCSSPSLSASPGSPARPQIRQKIENKPLQEQLPVNQIKTEPVDYEFKPIVVASGINCSTPLQNGVFSGGSPLQATSSPQGVVQAVVLPTVGLVSPISINLSDIQNVLKVAVDGNVIRQVLENNHANLASKEQETISNASIQQAGHSLISAISLPLVDQDGTTKIIINYSLEQPSQLQVVPQNLKKEHSVPTNSCKNEKLPEDLTVKSEKDKNFEGETNDSTCLLCDDCPGDLNALQELKHYETKNPPQLPQSSGTEAEKPSSPAPSETGENNLSPGQPPLKNLLSLLKAYYALNAQPSAEELSKIADSVNLPLDVVKKWFEKMQAGQISVQSSGPSSPEQVKISSPTDNDDQAATTNESEPQNSTNNSQNPANTSKSQTSSGGSTQNGSRSSTPSPSPLNLSSSRNSQGYTYTAEGVQEEPQMEPLDLSLPKQHGELLERSTITSVYQNSVYSVQEEPLNLTCAKKEPQKDNSITDSDPIVNVIPPSANPINIAIPTVTAQLPTIVAIADQNSVPCLRALAANKQTILIPQVAYTYSTTVSPAVQETPPKQTQANGSQDERQDTSSEGVSNVEDQNDSDSTPPKKKMRKTENGMYACDLCDKIFQKSSSLLRHKYEHTGKRPHECGICKKAFKHKHHLIEHMRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKREAEERDSTEQEEVGQEVLSSEHAGARASPSQIDSDERESLTREEEEDSEKEEEEEEEKDVEGLQEEKECRKLQDVEEEEEVEEEEEEEEGKTEGNKNDDVVNRASNAEPEVIQSNGQVSEEKTNKA

Target ORF information:

RefSeq Version	XM_015281304.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus zinc finger E-box binding homeobox 1 (ZEB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281304.1

ORF Insert Sequence:

ATGGCGGATG GCCCCAGGTG TAAGCGCAGG AAGCAGGCGA ACCCGAGGCG CAACAACGTC 
ACCAATTACA ATAACGTGAT AGAAGCAAAC TCAGATTCAG ATGACGAAGA TAAATTGCAT 
ATAGTGGAAG AAGAAAGTAT AACTGATGCA GCAGACTGCG ATGCAAGTGT GCCAGAAGAT 
GACTTGCCAA CAGACCACAC CGTGTTACCA GAAAACAGTG AGAGGGAAGG GAGCACAAAC 
AGCTGCTGGG AAGATGAAGC AGGAAAAGAA ACAAAGGAAA TCCTGGGGCC TGAAGCTCAG 
TCAGATGAAG TTGGATGTAC AGTAAAAGAA GATGAATGTG ATTCTGATGC AGAAAATGAA 
CAGAACCATG ACCCTAATGT TGAAGAATTC CTTCAACAAG AAGATACAGC TGTTATTTAC 
CCTGAAGCAC CTGAGGAGGA CCAGAGACAA GGCACACCAG AAGCTAGTGG TCAGGATGAA 
AATGGAACGC CCGATGCATT TTCCCAGCTG CTCACTTGCC CGTACTGTGA CAGAGGGTAC 
AAACGCTTCA CCTCTCTGAA GGAACACATT AAATACCGCC ATGAAAAGAA CGAGGATAAC 
TTCAGTTGCT CCCTCTGCAG TTACACGTTT GCGTATAGAA CACAGCTGGA CCGCCACATG 
ACGTCGCACA AGTCGGGACG AGATCAGAGA CATGTGACGC AGTCCAGTGG TAATCGAAAA 
TTCAAGTGCA CTGAATGTGG AAAAGCTTTC AAATATAAAC ATCATCTAAA GGAACACCTA 
CGAATCCACA GTGGAGAGAA GCCATATGAG TGCCCAAACT GCAAGAAACG TTTTTCCCAT 
TCTGGTTCAT ACAGCTCACA CATAAGCAGT AAGAAGTGTA TTGGTTTGAT GCCCGTGAAT 
GGTCGAGCTC GGTCAGGGCT CAAGATGTCT CAGTGCTCCT CCCCTTCCCT TTCTGCATCG 
CCCGGTAGCC CAGCAAGACC ACAGATACGA CAAAAGATAG AAAATAAACC CTTGCAAGAG 
CAACTTCCTG TTAACCAAAT TAAAACTGAA CCTGTGGATT ATGAATTCAA GCCCATAGTG 
GTTGCTTCAG GAATTAATTG TTCGACCCCT TTGCAGAATG GGGTTTTTAG TGGTGGTAGC 
CCGTTGCAGG CAACCAGTTC TCCTCAGGGT GTGGTGCAAG CTGTTGTTCT GCCAACAGTG 
GGTCTGGTGT CTCCCATAAG CATCAACTTA AGTGACATTC AAAATGTACT TAAAGTGGCA 
GTGGATGGTA ATGTAATAAG GCAAGTATTG GAAAACAATC ACGCTAATCT TGCATCCAAA 
GAACAAGAAA CAATCAGCAA TGCATCTATA CAACAAGCTG GCCATTCCCT CATTTCAGCT 
ATCAGTCTTC CTTTGGTTGA CCAAGATGGG ACAACCAAAA TTATCATCAA CTACAGCTTG 
GAGCAGCCAA GTCAACTTCA GGTTGTTCCC CAAAATCTAA AAAAAGAACA TTCTGTTCCT 
ACAAACAGTT GCAAAAATGA AAAGTTACCA GAAGATCTTA CGGTGAAGTC TGAGAAAGAT 
AAAAACTTTG AAGGAGAGAC CAACGATAGC ACTTGTCTTC TTTGTGATGA CTGTCCAGGA 
GATCTTAATG CACTTCAAGA ATTAAAGCAC TATGAAACAA AAAATCCTCC TCAGCTTCCC 
CAGTCCAGTG GAACAGAAGC TGAGAAGCCC AGCTCCCCTG CCCCATCAGA AACTGGGGAG 
AACAACTTAT CTCCTGGTCA GCCACCCTTA AAGAACCTTT TATCGCTCCT AAAAGCATAT 
TATGCATTAA ATGCACAACC AAGCGCAGAA GAGCTTTCAA AAATTGCCGA TTCCGTGAAC 
CTACCACTGG ATGTGGTAAA AAAGTGGTTT GAAAAAATGC AAGCTGGACA AATTTCTGTG 
CAGTCTTCTG GACCATCTTC TCCTGAACAA GTTAAAATAA GCAGTCCCAC AGATAACGAT 
GATCAAGCAG CAACTACAAA TGAGAGTGAA CCCCAGAACA GCACAAACAA CTCACAAAAT 
CCGGCCAATA CAAGTAAATC TCAGACTTCA TCAGGGGGAT CAACTCAGAA TGGTTCGCGC 
AGTAGCACGC CATCCCCATC ACCACTAAAC CTTTCTTCAT CAAGAAATTC ACAGGGTTAT 
ACGTACACAG CAGAGGGTGT ACAAGAAGAG CCACAAATGG AACCTCTTGA CCTTTCACTA 
CCAAAGCAAC ATGGAGAACT GTTGGAAAGA TCTACCATAA CTAGTGTTTA CCAGAACAGT 
GTTTATTCTG TCCAGGAAGA ACCTTTGAAC TTAACTTGTG CAAAAAAAGA ACCACAAAAG 
GACAACAGTA TTACAGACTC TGATCCTATT GTAAATGTAA TCCCACCAAG TGCCAATCCC 
ATAAATATTG CTATACCTAC AGTCACTGCC CAGTTACCTA CAATTGTTGC CATTGCTGAC 
CAGAACAGTG TTCCATGCTT GAGAGCTCTT GCTGCCAATA AGCAAACCAT TTTGATTCCC 
CAGGTGGCTT ATACGTACTC TACTACAGTT AGTCCTGCAG TTCAGGAAAC ACCACCAAAA 
CAGACCCAAG CCAACGGAAG TCAGGACGAA AGGCAGGACA CTAGCTCAGA AGGAGTATCG 
AATGTAGAAG ATCAAAATGA TTCTGATTCA ACACCCCCAA AGAAAAAGAT GAGAAAGACA 
GAAAATGGGA TGTATGCATG TGATTTGTGT GACAAAATAT TCCAGAAGAG CAGCTCATTA 
TTGAGACATA AGTATGAACA CACAGGTAAA AGACCTCATG AGTGTGGAAT CTGTAAAAAA 
GCCTTCAAAC ACAAACACCA TTTGATCGAA CACATGCGAC TGCATTCTGG GGAGAAACCC 
TACCAATGTG ACAAATGCGG GAAGCGCTTC TCACACTCGG GGTCGTACTC TCAGCACATG 
AATCATCGCT ACTCCTATTG CAAAAGAGAG GCAGAGGAGC GTGACAGCAC AGAGCAGGAG 
GAGGTGGGAC AGGAGGTGCT CAGCAGCGAG CATGCTGGTG CCAGGGCATC GCCATCGCAG 
ATCGACTCCG ATGAGAGAGA GAGTCTAACC AGGGAAGAAG AGGAAGATAG TGAAAAAGAG 
GAAGAGGAGG AGGAAGAAAA GGACGTAGAA GGACTTCAGG AAGAAAAAGA ATGTAGAAAA 
CTACAAGATG TAGAGGAGGA AGAAGAAGTA GAAGAAGAAG AAGAAGAAGA GGAAGGGAAA 
ACTGAAGGTA ACAAGAACGA TGATGTTGTA AATCGAGCAA GCAATGCAGA ACCAGAAGTT 
ATACAGAGCA ATGGGCAGGT GTCAGAAGAA AAAACAAATA AAGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZEB1 ( XM_015281304.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015136790.1 Gallus gallus zinc finger E-box binding homeobox 1 (ZEB1), transcript variant X1, mRNA.

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CloneID	OGa31494
Clone ID Related Accession (Same CDS sequence)	XM_015281304.1 , XM_015281304.2
Accession Version	XM_015281304.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	zinc finger E-box-binding homeobox 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015281304.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG GCCCCAGGTG TAAGCGCAGG AAGCAGGCGA ACCCGAGGCG CAACAACGTC 
ACCAATTACA ATAACGTGAT AGAAGCAAAC TCAGATTCAG ATGACGAAGA TAAATTGCAT 
ATAGTGGAAG AAGAAAGTAT AACTGATGCA GCAGACTGCG ATGCAAGTGT GCCAGAAGAT 
GACTTGCCAA CAGACCACAC CGTGTTACCA GAAAACAGTG AGAGGGAAGG GAGCACAAAC 
AGCTGCTGGG AAGATGAAGC AGGAAAAGAA ACAAAGGAAA TCCTGGGGCC TGAAGCTCAG 
TCAGATGAAG TTGGATGTAC AGTAAAAGAA GATGAATGTG ATTCTGATGC AGAAAATGAA 
CAGAACCATG ACCCTAATGT TGAAGAATTC CTTCAACAAG AAGATACAGC TGTTATTTAC 
CCTGAAGCAC CTGAGGAGGA CCAGAGACAA GGCACACCAG AAGCTAGTGG TCAGGATGAA 
AATGGAACGC CCGATGCATT TTCCCAGCTG CTCACTTGCC CGTACTGTGA CAGAGGGTAC 
AAACGCTTCA CCTCTCTGAA GGAACACATT AAATACCGCC ATGAAAAGAA CGAGGATAAC 
TTCAGTTGCT CCCTCTGCAG TTACACGTTT GCGTATAGAA CACAGCTGGA CCGCCACATG 
ACGTCGCACA AGTCGGGACG AGATCAGAGA CATGTGACGC AGTCCAGTGG TAATCGAAAA 
TTCAAGTGCA CTGAATGTGG AAAAGCTTTC AAATATAAAC ATCATCTAAA GGAACACCTA 
CGAATCCACA GTGGAGAGAA GCCATATGAG TGCCCAAACT GCAAGAAACG TTTTTCCCAT 
TCTGGTTCAT ACAGCTCACA CATAAGCAGT AAGAAGTGTA TTGGTTTGAT GCCCGTGAAT 
GGTCGAGCTC GGTCAGGGCT CAAGATGTCT CAGTGCTCCT CCCCTTCCCT TTCTGCATCG 
CCCGGTAGCC CAGCAAGACC ACAGATACGA CAAAAGATAG AAAATAAACC CTTGCAAGAG 
CAACTTCCTG TTAACCAAAT TAAAACTGAA CCTGTGGATT ATGAATTCAA GCCCATAGTG 
GTTGCTTCAG GAATTAATTG TTCGACCCCT TTGCAGAATG GGGTTTTTAG TGGTGGTAGC 
CCGTTGCAGG CAACCAGTTC TCCTCAGGGT GTGGTGCAAG CTGTTGTTCT GCCAACAGTG 
GGTCTGGTGT CTCCCATAAG CATCAACTTA AGTGACATTC AAAATGTACT TAAAGTGGCA 
GTGGATGGTA ATGTAATAAG GCAAGTATTG GAAAACAATC ACGCTAATCT TGCATCCAAA 
GAACAAGAAA CAATCAGCAA TGCATCTATA CAACAAGCTG GCCATTCCCT CATTTCAGCT 
ATCAGTCTTC CTTTGGTTGA CCAAGATGGG ACAACCAAAA TTATCATCAA CTACAGCTTG 
GAGCAGCCAA GTCAACTTCA GGTTGTTCCC CAAAATCTAA AAAAAGAACA TTCTGTTCCT 
ACAAACAGTT GCAAAAATGA AAAGTTACCA GAAGATCTTA CGGTGAAGTC TGAGAAAGAT 
AAAAACTTTG AAGGAGAGAC CAACGATAGC ACTTGTCTTC TTTGTGATGA CTGTCCAGGA 
GATCTTAATG CACTTCAAGA ATTAAAGCAC TATGAAACAA AAAATCCTCC TCAGCTTCCC 
CAGTCCAGTG GAACAGAAGC TGAGAAGCCC AGCTCCCCTG CCCCATCAGA AACTGGGGAG 
AACAACTTAT CTCCTGGTCA GCCACCCTTA AAGAACCTTT TATCGCTCCT AAAAGCATAT 
TATGCATTAA ATGCACAACC AAGCGCAGAA GAGCTTTCAA AAATTGCCGA TTCCGTGAAC 
CTACCACTGG ATGTGGTAAA AAAGTGGTTT GAAAAAATGC AAGCTGGACA AATTTCTGTG 
CAGTCTTCTG GACCATCTTC TCCTGAACAA GTTAAAATAA GCAGTCCCAC AGATAACGAT 
GATCAAGCAG CAACTACAAA TGAGAGTGAA CCCCAGAACA GCACAAACAA CTCACAAAAT 
CCGGCCAATA CAAGTAAATC TCAGACTTCA TCAGGGGGAT CAACTCAGAA TGGTTCGCGC 
AGTAGCACGC CATCCCCATC ACCACTAAAC CTTTCTTCAT CAAGAAATTC ACAGGGTTAT 
ACGTACACAG CAGAGGGTGT ACAAGAAGAG CCACAAATGG AACCTCTTGA CCTTTCACTA 
CCAAAGCAAC ATGGAGAACT GTTGGAAAGA TCTACCATAA CTAGTGTTTA CCAGAACAGT 
GTTTATTCTG TCCAGGAAGA ACCTTTGAAC TTAACTTGTG CAAAAAAAGA ACCACAAAAG 
GACAACAGTA TTACAGACTC TGATCCTATT GTAAATGTAA TCCCACCAAG TGCCAATCCC 
ATAAATATTG CTATACCTAC AGTCACTGCC CAGTTACCTA CAATTGTTGC CATTGCTGAC 
CAGAACAGTG TTCCATGCTT GAGAGCTCTT GCTGCCAATA AGCAAACCAT TTTGATTCCC 
CAGGTGGCTT ATACGTACTC TACTACAGTT AGTCCTGCAG TTCAGGAAAC ACCACCAAAA 
CAGACCCAAG CCAACGGAAG TCAGGACGAA AGGCAGGACA CTAGCTCAGA AGGAGTATCG 
AATGTAGAAG ATCAAAATGA TTCTGATTCA ACACCCCCAA AGAAAAAGAT GAGAAAGACA 
GAAAATGGGA TGTATGCATG TGATTTGTGT GACAAAATAT TCCAGAAGAG CAGCTCATTA 
TTGAGACATA AGTATGAACA CACAGGTAAA AGACCTCATG AGTGTGGAAT CTGTAAAAAA 
GCCTTCAAAC ACAAACACCA TTTGATCGAA CACATGCGAC TGCATTCTGG GGAGAAACCC 
TACCAATGTG ACAAATGCGG GAAGCGCTTC TCACACTCGG GGTCGTACTC TCAGCACATG 
AATCATCGCT ACTCCTATTG CAAAAGAGAG GCAGAGGAGC GTGACAGCAC AGAGCAGGAG 
GAGGTGGGAC AGGAGGTGCT CAGCAGCGAG CATGCTGGTG CCAGGGCATC GCCATCGCAG 
ATCGACTCCG ATGAGAGAGA GAGTCTAACC AGGGAAGAAG AGGAAGATAG TGAAAAAGAG 
GAAGAGGAGG AGGAAGAAAA GGACGTAGAA GGACTTCAGG AAGAAAAAGA ATGTAGAAAA 
CTACAAGATG TAGAGGAGGA AGAAGAAGTA GAAGAAGAAG AAGAAGAAGA GGAAGGGAAA 
ACTGAAGGTA ACAAGAACGA TGATGTTGTA AATCGAGCAA GCAATGCAGA ACCAGAAGTT 
ATACAGAGCA ATGGGCAGGT GTCAGAAGAA AAAACAAATA AAGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015136790.1
CDS	64..3411
Translation	MADGPRCKRRKQANPRRNNVTNYNNVIEANSDSDDEDKLHIVEEESITDAADCDASVPEDDLPTDHTVLPENSEREGSTNSCWEDEAGKETKEILGPEAQSDEVGCTVKEDECDSDAENEQNHDPNVEEFLQQEDTAVIYPEAPEEDQRQGTPEASGQDENGTPDAFSQLLTCPYCDRGYKRFTSLKEHIKYRHEKNEDNFSCSLCSYTFAYRTQLDRHMTSHKSGRDQRHVTQSSGNRKFKCTECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCIGLMPVNGRARSGLKMSQCSSPSLSASPGSPARPQIRQKIENKPLQEQLPVNQIKTEPVDYEFKPIVVASGINCSTPLQNGVFSGGSPLQATSSPQGVVQAVVLPTVGLVSPISINLSDIQNVLKVAVDGNVIRQVLENNHANLASKEQETISNASIQQAGHSLISAISLPLVDQDGTTKIIINYSLEQPSQLQVVPQNLKKEHSVPTNSCKNEKLPEDLTVKSEKDKNFEGETNDSTCLLCDDCPGDLNALQELKHYETKNPPQLPQSSGTEAEKPSSPAPSETGENNLSPGQPPLKNLLSLLKAYYALNAQPSAEELSKIADSVNLPLDVVKKWFEKMQAGQISVQSSGPSSPEQVKISSPTDNDDQAATTNESEPQNSTNNSQNPANTSKSQTSSGGSTQNGSRSSTPSPSPLNLSSSRNSQGYTYTAEGVQEEPQMEPLDLSLPKQHGELLERSTITSVYQNSVYSVQEEPLNLTCAKKEPQKDNSITDSDPIVNVIPPSANPINIAIPTVTAQLPTIVAIADQNSVPCLRALAANKQTILIPQVAYTYSTTVSPAVQETPPKQTQANGSQDERQDTSSEGVSNVEDQNDSDSTPPKKKMRKTENGMYACDLCDKIFQKSSSLLRHKYEHTGKRPHECGICKKAFKHKHHLIEHMRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKREAEERDSTEQEEVGQEVLSSEHAGARASPSQIDSDERESLTREEEEDSEKEEEEEEEKDVEGLQEEKECRKLQDVEEEEEVEEEEEEEEGKTEGNKNDDVVNRASNAEPEVIQSNGQVSEEKTNKA

Target ORF information:

RefSeq Version	XM_015281304.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus zinc finger E-box binding homeobox 1 (ZEB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281304.2

ORF Insert Sequence:

ATGGCGGATG GCCCCAGGTG TAAGCGCAGG AAGCAGGCGA ACCCGAGGCG CAACAACGTC 
ACCAATTACA ATAACGTGAT AGAAGCAAAC TCAGATTCAG ATGACGAAGA TAAATTGCAT 
ATAGTGGAAG AAGAAAGTAT AACTGATGCA GCAGACTGCG ATGCAAGTGT GCCAGAAGAT 
GACTTGCCAA CAGACCACAC CGTGTTACCA GAAAACAGTG AGAGGGAAGG GAGCACAAAC 
AGCTGCTGGG AAGATGAAGC AGGAAAAGAA ACAAAGGAAA TCCTGGGGCC TGAAGCTCAG 
TCAGATGAAG TTGGATGTAC AGTAAAAGAA GATGAATGTG ATTCTGATGC AGAAAATGAA 
CAGAACCATG ACCCTAATGT TGAAGAATTC CTTCAACAAG AAGATACAGC TGTTATTTAC 
CCTGAAGCAC CTGAGGAGGA CCAGAGACAA GGCACACCAG AAGCTAGTGG TCAGGATGAA 
AATGGAACGC CCGATGCATT TTCCCAGCTG CTCACTTGCC CGTACTGTGA CAGAGGGTAC 
AAACGCTTCA CCTCTCTGAA GGAACACATT AAATACCGCC ATGAAAAGAA CGAGGATAAC 
TTCAGTTGCT CCCTCTGCAG TTACACGTTT GCGTATAGAA CACAGCTGGA CCGCCACATG 
ACGTCGCACA AGTCGGGACG AGATCAGAGA CATGTGACGC AGTCCAGTGG TAATCGAAAA 
TTCAAGTGCA CTGAATGTGG AAAAGCTTTC AAATATAAAC ATCATCTAAA GGAACACCTA 
CGAATCCACA GTGGAGAGAA GCCATATGAG TGCCCAAACT GCAAGAAACG TTTTTCCCAT 
TCTGGTTCAT ACAGCTCACA CATAAGCAGT AAGAAGTGTA TTGGTTTGAT GCCCGTGAAT 
GGTCGAGCTC GGTCAGGGCT CAAGATGTCT CAGTGCTCCT CCCCTTCCCT TTCTGCATCG 
CCCGGTAGCC CAGCAAGACC ACAGATACGA CAAAAGATAG AAAATAAACC CTTGCAAGAG 
CAACTTCCTG TTAACCAAAT TAAAACTGAA CCTGTGGATT ATGAATTCAA GCCCATAGTG 
GTTGCTTCAG GAATTAATTG TTCGACCCCT TTGCAGAATG GGGTTTTTAG TGGTGGTAGC 
CCGTTGCAGG CAACCAGTTC TCCTCAGGGT GTGGTGCAAG CTGTTGTTCT GCCAACAGTG 
GGTCTGGTGT CTCCCATAAG CATCAACTTA AGTGACATTC AAAATGTACT TAAAGTGGCA 
GTGGATGGTA ATGTAATAAG GCAAGTATTG GAAAACAATC ACGCTAATCT TGCATCCAAA 
GAACAAGAAA CAATCAGCAA TGCATCTATA CAACAAGCTG GCCATTCCCT CATTTCAGCT 
ATCAGTCTTC CTTTGGTTGA CCAAGATGGG ACAACCAAAA TTATCATCAA CTACAGCTTG 
GAGCAGCCAA GTCAACTTCA GGTTGTTCCC CAAAATCTAA AAAAAGAACA TTCTGTTCCT 
ACAAACAGTT GCAAAAATGA AAAGTTACCA GAAGATCTTA CGGTGAAGTC TGAGAAAGAT 
AAAAACTTTG AAGGAGAGAC CAACGATAGC ACTTGTCTTC TTTGTGATGA CTGTCCAGGA 
GATCTTAATG CACTTCAAGA ATTAAAGCAC TATGAAACAA AAAATCCTCC TCAGCTTCCC 
CAGTCCAGTG GAACAGAAGC TGAGAAGCCC AGCTCCCCTG CCCCATCAGA AACTGGGGAG 
AACAACTTAT CTCCTGGTCA GCCACCCTTA AAGAACCTTT TATCGCTCCT AAAAGCATAT 
TATGCATTAA ATGCACAACC AAGCGCAGAA GAGCTTTCAA AAATTGCCGA TTCCGTGAAC 
CTACCACTGG ATGTGGTAAA AAAGTGGTTT GAAAAAATGC AAGCTGGACA AATTTCTGTG 
CAGTCTTCTG GACCATCTTC TCCTGAACAA GTTAAAATAA GCAGTCCCAC AGATAACGAT 
GATCAAGCAG CAACTACAAA TGAGAGTGAA CCCCAGAACA GCACAAACAA CTCACAAAAT 
CCGGCCAATA CAAGTAAATC TCAGACTTCA TCAGGGGGAT CAACTCAGAA TGGTTCGCGC 
AGTAGCACGC CATCCCCATC ACCACTAAAC CTTTCTTCAT CAAGAAATTC ACAGGGTTAT 
ACGTACACAG CAGAGGGTGT ACAAGAAGAG CCACAAATGG AACCTCTTGA CCTTTCACTA 
CCAAAGCAAC ATGGAGAACT GTTGGAAAGA TCTACCATAA CTAGTGTTTA CCAGAACAGT 
GTTTATTCTG TCCAGGAAGA ACCTTTGAAC TTAACTTGTG CAAAAAAAGA ACCACAAAAG 
GACAACAGTA TTACAGACTC TGATCCTATT GTAAATGTAA TCCCACCAAG TGCCAATCCC 
ATAAATATTG CTATACCTAC AGTCACTGCC CAGTTACCTA CAATTGTTGC CATTGCTGAC 
CAGAACAGTG TTCCATGCTT GAGAGCTCTT GCTGCCAATA AGCAAACCAT TTTGATTCCC 
CAGGTGGCTT ATACGTACTC TACTACAGTT AGTCCTGCAG TTCAGGAAAC ACCACCAAAA 
CAGACCCAAG CCAACGGAAG TCAGGACGAA AGGCAGGACA CTAGCTCAGA AGGAGTATCG 
AATGTAGAAG ATCAAAATGA TTCTGATTCA ACACCCCCAA AGAAAAAGAT GAGAAAGACA 
GAAAATGGGA TGTATGCATG TGATTTGTGT GACAAAATAT TCCAGAAGAG CAGCTCATTA 
TTGAGACATA AGTATGAACA CACAGGTAAA AGACCTCATG AGTGTGGAAT CTGTAAAAAA 
GCCTTCAAAC ACAAACACCA TTTGATCGAA CACATGCGAC TGCATTCTGG GGAGAAACCC 
TACCAATGTG ACAAATGCGG GAAGCGCTTC TCACACTCGG GGTCGTACTC TCAGCACATG 
AATCATCGCT ACTCCTATTG CAAAAGAGAG GCAGAGGAGC GTGACAGCAC AGAGCAGGAG 
GAGGTGGGAC AGGAGGTGCT CAGCAGCGAG CATGCTGGTG CCAGGGCATC GCCATCGCAG 
ATCGACTCCG ATGAGAGAGA GAGTCTAACC AGGGAAGAAG AGGAAGATAG TGAAAAAGAG 
GAAGAGGAGG AGGAAGAAAA GGACGTAGAA GGACTTCAGG AAGAAAAAGA ATGTAGAAAA 
CTACAAGATG TAGAGGAGGA AGAAGAAGTA GAAGAAGAAG AAGAAGAAGA GGAAGGGAAA 
ACTGAAGGTA ACAAGAACGA TGATGTTGTA AATCGAGCAA GCAATGCAGA ACCAGAAGTT 
ATACAGAGCA ATGGGCAGGT GTCAGAAGAA AAAACAAATA AAGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZEB1 ( NM_205131.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990462.1 Gallus gallus zinc finger E-box binding homeobox 1 (ZEB1), mRNA.

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CloneID	OGa00405
Clone ID Related Accession (Same CDS sequence)	NM_205131.1
Accession Version	NM_205131.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-10
Organism	Gallus gallus(chicken)
Product	zinc finger E-box-binding homeobox 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D14313.1. ##Evidence-Data-START## Transcript exon combination :: D14313.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCGGATG GCCCCAGGTG TAAGCGCAGG AAGCAGGCGA ACCCGAGGCG CAACAACGTC 
ACCAATTACA ATAACGTGAT AGAAGCAAAC TCAGATTCAG ATGACGAAGA TAAATTGCAT 
ATAGTGGAAG AAGAAAGTAT AACTGATGCA GCAGACTGCG ATGCAAGTGT GCCAGAAGAT 
GACTTGCCAA CAGACCACAC CGTGTTACCA GAAAACAGTG AGAGGGAAGG GAGCACAAAC 
AGCTGCTGGG AAGATGAAGG AAAAGAAACA AAGGAAATCC TGGGGCCTGA AGCTCAGTCA 
GATGAAGTTG GATGTACAGT AAAAGAAGAT GAATGTGATT CTGATGCAGA AAATGAACAG 
AACCATGACC CTAATGTTGA AGAATTCCTT CAACAAGAAG ATACAGCTGT TATTTACCCT 
GAAGCACCTG AGGAGGACCA GAGACAAGGC ACACCAGAAG CTAGTGGTCA GGATGAAAAT 
GGAACGCCCG ATGCATTTTC CCAGCTGCTC ACCTGCCCGT ACTGTGACAG AGGGTACAAA 
CGCTTCACCT CTCTGAAGGA ACACATTAAA TACCGCCATG AAAAGAACGA GGATAACTTC 
AGTTGCTCCC TCTGCAGTTA CACGTTTGCG TATAGAACAC AGCTGGACCG CCACATGACG 
TCGCACAAGT CAGGACGAGA TCAGAGACAT GTGACGCAGT CCAGTGGTAA TCGAAAATTC 
AAGTGCACTG AATGTGGAAA AGCTTTTAAA TATAAACATC ATCTAAAGGA ACACCTACGA 
ATTCACAGTG GAGAGAAGCC ATATGAGTGC CCAAACTGCA AGAAACGTTT TTCCCATTCT 
GGTTCATACA GCTCACACAT AAGCAGTAAG AAGTGTATTG GTTTGATGCC CGTAAAGGGT 
CGAGCTCGGT CAGGGCTCAA GACGTCTCAG TGCTCCTCCC CTTCCCTTTC TGCATCGCCC 
GGTAGCCCAG CAAGACCACA GATACGACAA AAGATAGAAA ATAAACCCTT GCAAGAGCAA 
CTTCCTGTTA ACCAAATTAA AACTGAACCT GTGGATTATG AATTCAAGCC CATAGTGGTT 
GCTTCAGGAA TTAATTGTTC GACCCCTTTG CAGAATGGGG TTTTTAGTGG TGGTAGCCCG 
TTGCAGGCAA CCAGTTCTCC TCAGGGTGTG GTGCAAGCTG TTGTTCTGCC AACAGTGGGT 
CTGGTGTCTC CCATAAGCAT CAACTTAAGT GACATTCAAA ATGTACTTAA AGTGGCAGTG 
GATGGTAATG TAATAAGGCA AGTATTGGAA AACAATCACG CTAATCTTGC ATCCAAAGAA 
CAAGAAACAA TCAGCAATGC ATCTATACAA CAAGCTGGCC ATTCCCTCAT TTCAGCTATC 
AGTCTTCCTT TGGTTGACCA AGATGGGACA ACCAAAATTA TCATCAACTA CAGCTTGGAG 
CAGCCAAGTC AACTTCAGGT TGTTCCCCAA AATCTAAAAA AAGAACATTC TGTTCCTACA 
AACAGTTGCA AAAATGAAAA GTTACCAGAA GATCTTACGG TGAAGTCTGA GAAAGATAAA 
AACTTTGAAG GAGAGACCAA CGATAGCACT TGTCTTCTTT GTGATGACTG TCCAGGAGAT 
CTTAATGCAC TTCAAGAATT AAAGCACTAT GAAACAAAAA ATCCTCCTCA GCTTCCCCAG 
TCCAGTGGAA CAGAAGCTGA GAAGCCCAGC TCCCCTGCCC CATCAGAAAC TGGGGAGAAC 
AACTTATCTC CTGGTCAGCC ACCCTTAAAG AACCTTTTAT CGCTCCTAAA AGCATATTAT 
GCATTAAATG CACAACCAAG CGCAGAAGAG CTTTCAAAAA TCGCCGATTC CGTGAACCTA 
CCACTGGATG TGGTAAAAAA GTGGTTTGAA AAAATGCAAG CTGGACAAAT TTCTGTGCAG 
TCTTCTGGAC CATCTTCTCC TGAACAAGTT AAAATAAGCA GTCCCACAGA TAACGATGAT 
CAAGCAGCAA CTACAAATGA GAGTGAACCC CAGAACAGCA CAAACAACTC ACAAAATCCG 
GCCAATACAA GTAAATCTCA GACTTCATCA GGGGGATCAA CTCAGAATGG TTCGCGCAGT 
AGCACGCCAT CCCCATCACC ACTAAACCTT TCTTCATCAA GAAATTCACA GGGTTATACG 
TACACAGCAG AGGGTGTACA AGAAGAGCCA CAAATGGAAC CTCTTGACCT TTCACTACCA 
AAGCAACATG GAGAACTGTT GGAAAGATCT ACCATAACTA GTGTTTACCA GAACAGTGTT 
TATTCTGTCC AGGAAGAACC TTTGAACTTA ACTTGTGCAA AAAAAGAACC ACAAAAGGAC 
AACAGTATTA CAGACTCTGA TCCTATTGTA AATGTAATCC CACCAAGTGC CAATCCCATA 
AATATTGCTA TACCTACAGT CACTGCCCAG TTACCTACAA TTGTTGCCAT TGCTGACCAG 
AACAGTGTTC CATGCTTGAG AGCTCTTGCT GCCAATAAGC AAACCATTTT GATTCCCCAG 
GTGGCTTATA CGTACTCTAC TACAGTTAGT CCTGCAGTTC AGGAAACACC ACCAAAACAG 
ACCCAAGCCA ACGGAAGTCA GGACGAAAGG CAGGACACTA GCTCAGAAGG AGTATCGAAT 
GTAGAAGATC AAAATGATTC TGATTCAACA CCCCCAAAGA AAAAGATGAG AAAGACAGAA 
AATGGGATGT ATGCATGTGA TTTGTGTGAC AAAATATTCC AGAAGAGCAG CTCATTATTG 
AGACATAAGT ATGAACACAC AGGTAAAAGA CCTCATGAGT GTGGAATCTG TAAAAAAGCC 
TTCAAACACA AACACCATTT GATCGAACAC ATGCGACTGC ATTCTGGGGA GAAACCCTAC 
CAATGTGACA AATGCGGGAA GCGCTTCTCA CACTCGGGGT CGTACTCTCA GCACATGAAT 
CATCGCTACT CCTATTGCAA AAGAGAGGCA GAGGAGCGTG ACAGCACAGA GCAGGAGGAG 
GTGGGACAGG AGGTGCTCAG CAGCGAGCAT GCTGGTGCCA GAGCATCGCC ATCGCAGATC 
GACTCCGATG AGAGAGAGAG TCTAACCAGG GAAGAAGAGG AAGATAGTGA AAAAGAGGAA 
GAGGAGGAGG AAGAAAAGGA CGTAGAAGGA CTTCAGGAAG AAAAAGAATG TAGAAAACTA 
CAAGATGTAG AGGAGGAAGA AGAAGTAGAA GAAGAAGAAG AAGAAGAGGA AGGGAAAACT 
GAAGGTAACA AGAACGATGA CGTTGTAAAT CGAGCAAGCA ATGCAGAACC AGAAGTTATA 
CAGAGCAATG GGCAGGTGTC AGAAGAAAAA ACAAATAAAG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990462.1
CDS	12..3356
Translation	MADGPRCKRRKQANPRRNNVTNYNNVIEANSDSDDEDKLHIVEEESITDAADCDASVPEDDLPTDHTVLPENSEREGSTNSCWEDEGKETKEILGPEAQSDEVGCTVKEDECDSDAENEQNHDPNVEEFLQQEDTAVIYPEAPEEDQRQGTPEASGQDENGTPDAFSQLLTCPYCDRGYKRFTSLKEHIKYRHEKNEDNFSCSLCSYTFAYRTQLDRHMTSHKSGRDQRHVTQSSGNRKFKCTECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCIGLMPVKGRARSGLKTSQCSSPSLSASPGSPARPQIRQKIENKPLQEQLPVNQIKTEPVDYEFKPIVVASGINCSTPLQNGVFSGGSPLQATSSPQGVVQAVVLPTVGLVSPISINLSDIQNVLKVAVDGNVIRQVLENNHANLASKEQETISNASIQQAGHSLISAISLPLVDQDGTTKIIINYSLEQPSQLQVVPQNLKKEHSVPTNSCKNEKLPEDLTVKSEKDKNFEGETNDSTCLLCDDCPGDLNALQELKHYETKNPPQLPQSSGTEAEKPSSPAPSETGENNLSPGQPPLKNLLSLLKAYYALNAQPSAEELSKIADSVNLPLDVVKKWFEKMQAGQISVQSSGPSSPEQVKISSPTDNDDQAATTNESEPQNSTNNSQNPANTSKSQTSSGGSTQNGSRSSTPSPSPLNLSSSRNSQGYTYTAEGVQEEPQMEPLDLSLPKQHGELLERSTITSVYQNSVYSVQEEPLNLTCAKKEPQKDNSITDSDPIVNVIPPSANPINIAIPTVTAQLPTIVAIADQNSVPCLRALAANKQTILIPQVAYTYSTTVSPAVQETPPKQTQANGSQDERQDTSSEGVSNVEDQNDSDSTPPKKKMRKTENGMYACDLCDKIFQKSSSLLRHKYEHTGKRPHECGICKKAFKHKHHLIEHMRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKREAEERDSTEQEEVGQEVLSSEHAGARASPSQIDSDERESLTREEEEDSEKEEEEEEEKDVEGLQEEKECRKLQDVEEEEEVEEEEEEEEGKTEGNKNDDVVNRASNAEPEVIQSNGQVSEEKTNKA

Target ORF information:

RefSeq Version	NM_205131.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus zinc finger E-box binding homeobox 1 (ZEB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205131.1

ORF Insert Sequence:

ATGGCGGATG GCCCCAGGTG TAAGCGCAGG AAGCAGGCGA ACCCGAGGCG CAACAACGTC 
ACCAATTACA ATAACGTGAT AGAAGCAAAC TCAGATTCAG ATGACGAAGA TAAATTGCAT 
ATAGTGGAAG AAGAAAGTAT AACTGATGCA GCAGACTGCG ATGCAAGTGT GCCAGAAGAT 
GACTTGCCAA CAGACCACAC CGTGTTACCA GAAAACAGTG AGAGGGAAGG GAGCACAAAC 
AGCTGCTGGG AAGATGAAGG AAAAGAAACA AAGGAAATCC TGGGGCCTGA AGCTCAGTCA 
GATGAAGTTG GATGTACAGT AAAAGAAGAT GAATGTGATT CTGATGCAGA AAATGAACAG 
AACCATGACC CTAATGTTGA AGAATTCCTT CAACAAGAAG ATACAGCTGT TATTTACCCT 
GAAGCACCTG AGGAGGACCA GAGACAAGGC ACACCAGAAG CTAGTGGTCA GGATGAAAAT 
GGAACGCCCG ATGCATTTTC CCAGCTGCTC ACCTGCCCGT ACTGTGACAG AGGGTACAAA 
CGCTTCACCT CTCTGAAGGA ACACATTAAA TACCGCCATG AAAAGAACGA GGATAACTTC 
AGTTGCTCCC TCTGCAGTTA CACGTTTGCG TATAGAACAC AGCTGGACCG CCACATGACG 
TCGCACAAGT CAGGACGAGA TCAGAGACAT GTGACGCAGT CCAGTGGTAA TCGAAAATTC 
AAGTGCACTG AATGTGGAAA AGCTTTTAAA TATAAACATC ATCTAAAGGA ACACCTACGA 
ATTCACAGTG GAGAGAAGCC ATATGAGTGC CCAAACTGCA AGAAACGTTT TTCCCATTCT 
GGTTCATACA GCTCACACAT AAGCAGTAAG AAGTGTATTG GTTTGATGCC CGTAAAGGGT 
CGAGCTCGGT CAGGGCTCAA GACGTCTCAG TGCTCCTCCC CTTCCCTTTC TGCATCGCCC 
GGTAGCCCAG CAAGACCACA GATACGACAA AAGATAGAAA ATAAACCCTT GCAAGAGCAA 
CTTCCTGTTA ACCAAATTAA AACTGAACCT GTGGATTATG AATTCAAGCC CATAGTGGTT 
GCTTCAGGAA TTAATTGTTC GACCCCTTTG CAGAATGGGG TTTTTAGTGG TGGTAGCCCG 
TTGCAGGCAA CCAGTTCTCC TCAGGGTGTG GTGCAAGCTG TTGTTCTGCC AACAGTGGGT 
CTGGTGTCTC CCATAAGCAT CAACTTAAGT GACATTCAAA ATGTACTTAA AGTGGCAGTG 
GATGGTAATG TAATAAGGCA AGTATTGGAA AACAATCACG CTAATCTTGC ATCCAAAGAA 
CAAGAAACAA TCAGCAATGC ATCTATACAA CAAGCTGGCC ATTCCCTCAT TTCAGCTATC 
AGTCTTCCTT TGGTTGACCA AGATGGGACA ACCAAAATTA TCATCAACTA CAGCTTGGAG 
CAGCCAAGTC AACTTCAGGT TGTTCCCCAA AATCTAAAAA AAGAACATTC TGTTCCTACA 
AACAGTTGCA AAAATGAAAA GTTACCAGAA GATCTTACGG TGAAGTCTGA GAAAGATAAA 
AACTTTGAAG GAGAGACCAA CGATAGCACT TGTCTTCTTT GTGATGACTG TCCAGGAGAT 
CTTAATGCAC TTCAAGAATT AAAGCACTAT GAAACAAAAA ATCCTCCTCA GCTTCCCCAG 
TCCAGTGGAA CAGAAGCTGA GAAGCCCAGC TCCCCTGCCC CATCAGAAAC TGGGGAGAAC 
AACTTATCTC CTGGTCAGCC ACCCTTAAAG AACCTTTTAT CGCTCCTAAA AGCATATTAT 
GCATTAAATG CACAACCAAG CGCAGAAGAG CTTTCAAAAA TCGCCGATTC CGTGAACCTA 
CCACTGGATG TGGTAAAAAA GTGGTTTGAA AAAATGCAAG CTGGACAAAT TTCTGTGCAG 
TCTTCTGGAC CATCTTCTCC TGAACAAGTT AAAATAAGCA GTCCCACAGA TAACGATGAT 
CAAGCAGCAA CTACAAATGA GAGTGAACCC CAGAACAGCA CAAACAACTC ACAAAATCCG 
GCCAATACAA GTAAATCTCA GACTTCATCA GGGGGATCAA CTCAGAATGG TTCGCGCAGT 
AGCACGCCAT CCCCATCACC ACTAAACCTT TCTTCATCAA GAAATTCACA GGGTTATACG 
TACACAGCAG AGGGTGTACA AGAAGAGCCA CAAATGGAAC CTCTTGACCT TTCACTACCA 
AAGCAACATG GAGAACTGTT GGAAAGATCT ACCATAACTA GTGTTTACCA GAACAGTGTT 
TATTCTGTCC AGGAAGAACC TTTGAACTTA ACTTGTGCAA AAAAAGAACC ACAAAAGGAC 
AACAGTATTA CAGACTCTGA TCCTATTGTA AATGTAATCC CACCAAGTGC CAATCCCATA 
AATATTGCTA TACCTACAGT CACTGCCCAG TTACCTACAA TTGTTGCCAT TGCTGACCAG 
AACAGTGTTC CATGCTTGAG AGCTCTTGCT GCCAATAAGC AAACCATTTT GATTCCCCAG 
GTGGCTTATA CGTACTCTAC TACAGTTAGT CCTGCAGTTC AGGAAACACC ACCAAAACAG 
ACCCAAGCCA ACGGAAGTCA GGACGAAAGG CAGGACACTA GCTCAGAAGG AGTATCGAAT 
GTAGAAGATC AAAATGATTC TGATTCAACA CCCCCAAAGA AAAAGATGAG AAAGACAGAA 
AATGGGATGT ATGCATGTGA TTTGTGTGAC AAAATATTCC AGAAGAGCAG CTCATTATTG 
AGACATAAGT ATGAACACAC AGGTAAAAGA CCTCATGAGT GTGGAATCTG TAAAAAAGCC 
TTCAAACACA AACACCATTT GATCGAACAC ATGCGACTGC ATTCTGGGGA GAAACCCTAC 
CAATGTGACA AATGCGGGAA GCGCTTCTCA CACTCGGGGT CGTACTCTCA GCACATGAAT 
CATCGCTACT CCTATTGCAA AAGAGAGGCA GAGGAGCGTG ACAGCACAGA GCAGGAGGAG 
GTGGGACAGG AGGTGCTCAG CAGCGAGCAT GCTGGTGCCA GAGCATCGCC ATCGCAGATC 
GACTCCGATG AGAGAGAGAG TCTAACCAGG GAAGAAGAGG AAGATAGTGA AAAAGAGGAA 
GAGGAGGAGG AAGAAAAGGA CGTAGAAGGA CTTCAGGAAG AAAAAGAATG TAGAAAACTA 
CAAGATGTAG AGGAGGAAGA AGAAGTAGAA GAAGAAGAAG AAGAAGAGGA AGGGAAAACT 
GAAGGTAACA AGAACGATGA CGTTGTAAAT CGAGCAAGCA ATGCAGAACC AGAAGTTATA 
CAGAGCAATG GGCAGGTGTC AGAAGAAAAA ACAAATAAAG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

miRNA-223 upregulated by MYOD inhibits myoblast proliferation by repressing IGF2 and facilitates myoblast differentiation by inhibiting ZEB1.
Cell death & disease8(10)e3094(2017 Oct)
Li G,Luo W,Abdalla BA,Ouyang H,Yu J,Hu F,Nie Q,Zhang X

Regulation of trunk neural crest delamination by ?EF1 and Sip1 in the chicken embryo.
Development, growth & differentiation58(2)205-14(2016 Feb)
Yasumi T,Inoue M,Maruhashi M,Kamachi Y,Higashi Y,Kondoh H,Uchikawa M

MicroRNA-200C and -150 play an important role in endothelial cell differentiation and vasculogenesis by targeting transcription repressor ZEB1.
Stem cells (Dayton, Ohio)31(9)1749-62(2013 Sep)
Luo Z,Wen G,Wang G,Pu X,Ye S,Xu Q,Wang W,Xiao Q

Negative modulation of the chicken infectious anemia virus promoter by COUP-TF1 and an E box-like element at the transcription start site binding deltaEF1.
The Journal of general virology89(Pt 12)2998-3003(2008 Dec)
Miller MM,Jarosinski KW,Schat KA

Organization of the gene encoding transcriptional repressor deltaEF1 and cross-species conservation of its domains.
Gene173(2)227-32(1996 Sep)
Sekido R,Takagi T,Okanami M,Moribe H,Yamamura M,Higashi Y,Kondoh H

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ZEB1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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