gRNAs
The following PTCH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTCH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OGa152959 | |
| Clone ID Related Accession (Same CDS sequence) | XM_025144670.1 | |
| Accession Version | XM_025144670.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3876bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-16 | |
| Organism | Gallus gallus(chicken) | |
| Product | protein patched homolog 1 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGTTTAACC CCCAGCTCAT GATTCAGACT CCACAGGAAG ATGGAACTAA TGTACTCACA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_025000438.1 |
| CDS | 122..3997 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_025144670.1 |
1 | ATGTTTAACC CCCAGCTCAT GATTCAGACT CCACAGGAAG ATGGAACTAA TGTACTCACA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa48039 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015280248.2 , XM_015280248.1 | |
| Accession Version | XM_015280248.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4182bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-16 | |
| Organism | Gallus gallus(chicken) | |
| Product | protein patched homolog 1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015280248.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAGGTCGC ACCCGGCCCC GTCAAGAGGC TCCCCGCCGG CTCCGGCAGC CATGGGGAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015135734.1 |
| CDS | 138..4319 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015280248.2 |
1 | ATGAGGTCGC ACCCGGCCCC GTCAAGAGGC TCCCCGCCGG CTCCGGCAGC CATGGGGAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa48039 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015280248.2 , XM_015280248.1 | |
| Accession Version | XM_015280248.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4182bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-01-03 | |
| Organism | Gallus gallus(chicken) | |
| Product | protein patched homolog 1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764318.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAGGTCGC ACCCGGCCCC GTCAAGAGGC TCCCCGCCGG CTCCGGCAGC CATGGGGAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015135734.1 |
| CDS | 113..4294 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015280248.1 |
1 | ATGAGGTCGC ACCCGGCCCC GTCAAGAGGC TCCCCGCCGG CTCCGGCAGC CATGGGGAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa28384 | |
| Clone ID Related Accession (Same CDS sequence) | NM_204960.2 | |
| Accession Version | NM_204960.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4332bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-02-24 | |
| Organism | Gallus gallus(chicken) | |
| Product | protein patched homolog 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC186361.2. On Mar 20, 2015 this sequence version replaced NM_204960.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: U40074.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGGCCTCGG CCGCTGACGC CTTAGAGCCG GAGAGCGGCA GCAGCACCGC GGGCGGCGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_990291.2 |
| CDS | 19..4350 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_204960.2 |
1 | ATGGCCTCGG CCGCTGACGC CTTAGAGCCG GAGAGCGGCA GCAGCACCGC GGGCGGCGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Genome duplication in the teleost fish Tetraodon nigroviridis reveals the early vertebrate proto-karyotype. |
Male-biased mutation rate and divergence in autosomal, z-linked and w-linked introns of chicken and Turkey. |
Biochemical evidence that patched is the Hedgehog receptor. |
Conservation in hedgehog signaling: induction of a chicken patched homolog by Sonic hedgehog in the developing limb. |
