PTCH1 cDNA ORF clone, Gallus gallus(chicken)

The following PTCH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTCH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa152959 XM_025144670.1
Latest version!
Gallus gallus patched 1 (PTCH1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa48039 XM_015280248.2
Latest version!
Gallus gallus patched 1 (PTCH1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa28384 NM_204960.2
Latest version!
Gallus gallus patched 1 (PTCH1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
View Old Accession Versions >>
OGa48039 XM_015280248.1 Gallus gallus patched 1 (PTCH1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa152959
    Clone ID Related Accession (Same CDS sequence) XM_025144670.1
    Accession Version XM_025144670.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3876bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein patched homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAACC CCCAGCTCAT GATTCAGACT CCACAGGAAG ATGGAACTAA TGTACTCACA 
    ACAGAAGCAC TCCGGCAGCA CCTTGACTCT GCACTTCAGG CCAGCAGAGT ACATGTTTAT
    ATGTACAACA GACAATGGAA GTTGGAACAT TTATGTTATA AATCAGGAGA ACTCATCACA
    GAAGCAGGCT ATATGGACCA GATTATAGAA TATCTTTATC CTTGCTTAAT TATCACTCCT
    TTGGACTGCT TCTGGGAGGG AGCAAAGCTA CAGTCTGGAA CTGCCTATCT ATTAGGGAAG
    CCTCCTTTGC AGTGGATCAA CTTTGACCCC TTAGAATTCT TGGAAGAGTT AAAGAAAATA
    AACTACCAAG TGGAGAGCTG GGAAGAAATG CTGAATAAAG CAGAGGTTGG TCATGGTTAT
    ATGGATCGAC CTTGCCTGAA TCCTGCTGAT CCTGATTGCC CAATCACAGC TCCCAATAAA
    AATTCTACCA AGCCTCTTGA TGTAGCCCTT GTTTTGAGTG GTGGATGCTA TGGACTGTCA
    AGAAAATACA TGCATTGGCA GGAAGAGTTA ATTATAGGTG GTACAGTCAA GAACAGTTCT
    GGTAAACTTG TCAGTGCCCA GGCTTTGCAA ACCATGTTTC AGTTAATGAC TCCTAAGCAA
    ATGTATGAGC ACTTCAAGGG ATATGAATAT GTTTCACATA TCAACTGGAA TGAGGATAAA
    GCAGCAGCAA TTCTGGAAGC CTGGCAGAGG ATGTATGTCG AGGTTGTTCA TCAAAGTGTT
    GCACAAAACT CTACTCAGAA GGTGCTTTCC TTTACTACAA CTACCCTGGA CGACATCCTA
    AAGTCATTTT CTGATGTCAG TGTTATCAGA GTAGCTAGTG GCTACTTACT AATGCTTGCC
    TACGCCTGTT TAACAATGCT GAGGTGGGAT TGTGCCAAGT CTCAAGGTGC CGTGGGGCTG
    GCAGGAGTCT TGTTGGTTGC ACTGTCAGTG GCTGCAGGCT TGGGTCTGTG CTCACTGATT
    GGAATTTCCT TTAATGCTGC CACAACTCAG GTTCTGCCTT TTCTTGCTCT CGGAGTTGGT
    GTAGATGATG TTTTCCTTCT AGCCCATGCG TTTAGTGAAA CAGGACAGAA TAAGAGAATT
    CCATTCGAGG ACAGAACAGG TGAATGTTTA AAGCGAACAG GAGCAAGTGT AGCCCTTACA
    TCTATCAGCA ATGTTACTGC ATTCTTTATG GCTGCCTTAA TCCCTATTCC TGCTTTACGA
    GCATTTTCAC TCCAAGCAGC AGTTGTGGTA GTATTCAACT TTGCAATGGT TCTGCTAATT
    TTTCCTGCTA TACTGAGCAT GGACCTTTAT CGTCGAGAAG ACAGGAGACT GGATATATTC
    TGCTGTTTTA CAAGTCCATG TGTCACCAGA GTGATTCAGA TCGAGCCTCA AGCGTATGCA
    GAAAATGACA ATATTTGTTA CAGTTCTCCA CCCCCGTACA GCAGTCACAG TTTTGCACAT
    GAAACTCAGA TTACAATGCA GTCTACAGTG CAGTTGCGCA CTGAATATGA TCCTCATACA
    CAAGCGTACT ACACCACTGC AGAGCCACGA TCAGAAATAT CAGTACAGCC TGTGACAGTG
    ACTCAAGATA GTCTTAGCTG CCAAAGCCCA GAAAGCGCAA GCTCAACAAG GGATTTGCTT
    TCCCAGTTCT CAGACTCCAG TGTGCATTGC CTTGAGCCAC CCTGTACGAA GTGGACACTC
    TCAACTTTTG CAGAAAAACA TTATGCTCCG TTCCTCCTAA AACCAAAAGC TAAGGTTGTG
    GTTATTTTTC TTTTCCTGGG CTTACTTGGG CTCAGCCTCT ATGGAACTAC CCGGGTGAGA
    GATGGACTAG ATCTCACAGA CATTGTACCA CGGGATACAC GGGAATATGA CTTTATTGCT
    GCGCAGTTCA AATACTTCTC ATTCTACAAT ATGTACATTG TGACACAGAA AGCAGACTAT
    CCAAATGTCC AGCACTTACT TTACGAACTT CACAGAAGTT TCAGCAATGT GACATATGTT
    TTGTTGGAAG GAGATAGGCA GCTTCCCAAA ATGTGGTTAC ATTACTTTCG AGACTGGCTG
    CAAGGACTCC AGGATGCATT TGACAGTGAC TGGGAAACTG GGAAAATCAC TTACAGCAAC
    TATAAAAATG GATCTGATGA TGCTGTTCTG GCTTATAAAC TCTTGGTACA AACAGGCAAC
    CGAGCAAAGC CCATTGACAT CAGTCAGTTG ACTAAACAGC GTCTGGTGGA TGCAGATGGA
    ATCATTAACC CAAATGCTTT CTACATCTAC CTGACAGCCT GGGTTAGCAA TGACCCTGTG
    GCCTATGCTG CCTCTCAAGC CAACATCCGG CCTCACCGCC CAGAATGGGT GCATGACAAA
    GCAGACTATA TGCCAGAAAC AAGGCTAAGA ATTCCAGCGG CTGAGCCCAT AGAATATGCT
    CAGTTTCCTT TCTACCTCAA TGGATTACGT GAGACTTCTG ACTTTGTGGA AGCTATTGAG
    AAAGTGAGAG CTATCTGCAA TAACTACACA AGCCTCGGGA TCGCTAGCTA CCCAAATGGT
    TACCCATTTC TATTCTGGGA GCAGTACATT GGGCTTCGTC ATTGGCTCCT CTTATCCATT
    AGCGTGGTTT TGGCTTGCAC GTTTCTGGTG TGTGCCCTCT TCCTCCTAAA CCCCTGGACA
    GCTGGGATCA TTGTGGTGGT GCTGGCTCTG ATGACTGTGG AGCTGTTTGG CATGATGGGT
    TTAATTGGAA TAAAACTAAG TGCAGTGCCT GTGGTTATCC TGATTGCTTC TGTTGGAATT
    GGTGTGGAAT TCACTGTTCA CATTGCTTTG GCATTTTTAA CTGCCATAGG AGATAAGAAC
    CGTAGGGCTG TCCTTGCATT GGAACATATG TTTGCACCAG TGTTGGATGG AGCTGTGTCC
    ACTCTTCTTG GAGTGTTAAT GCTTGCAGGA TCAGAGTTTG ATTTTATTGT CAGGTATTTC
    TTTGCTGTTT TGGCAATCTT AACCATTCTG GGAGTTCTCA ATGGATTGGT GCTGCTTCCT
    GTTCTTCTTT CTTTCTTTGG ACCATACCCT GAGGTTTCTC CAGCCTGTGG TCGAAACAGA
    TTACCTACAC CATCTCCTGA GCCACCTCCG AGTATTGTGA GGTTTGCACT GCCGCCTGGG
    CACACAAATA ATGGGTCAGA TTCGTCTGAT TCAGAGTACA GCTCTCAAAC CACCGTGTCA
    GGCATCAGTG AGGAGCTCCA TCATTATGAG GCCACCCAAA GCCCTGGGAT ACCAGTCCAT
    CAAGTAGTAG TGGAAGCCAC TGAGAATCCT GTCTTTGCAA GATCCACTGT GGTACAGCCA
    GAGTCAAGGC ATCAGTCAAG TCCCAGATTG CAATCAAATC CAGAGGCTGG CACACAGCAG
    GTGTGGCATC AGGGCAGACA ACCCAAGCAG GAAGTGAGGG AAGGACTGCG ACCACCTCCT
    TACCGCCCTC GCAGGGACGC TTTTGAAATT TCTACTGAAG GGCATTCAGG ACCTAGCAAT
    AAGGATCGCT TGAACCACAA GGCTCATTCT CATAACATGA GAAGCCCAGC ATTTGGTGCC
    ATGGGTGTTC CAGGATCTGC CTACTGCCAG CCTATCACTA CTGTGACTGC TTCGGCTTCA
    GTGACTGTTG CTGTCCACCC TGCTGTGCAC AGCCACAACT CTTGCAGAGG GAGCTTTCCA
    TCCTGTGAAG AATACAATGA AGATGACCGT GAGATGTTTG AGGACCCCCA TGTGCCTTTT
    AATGTGCGGT GTGAAAGAAG AAATTCTAAA GTAGAAGTTA TAGAGCTTCA GGATGTCGAA
    TGTGAGGAGA GGACAGCTGG CAAAATCTCA GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000438.1
    CDS122..3997
    Translation

    Target ORF information:

    RefSeq Version XM_025144670.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus patched 1 (PTCH1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025144670.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAACC CCCAGCTCAT GATTCAGACT CCACAGGAAG ATGGAACTAA TGTACTCACA 
    ACAGAAGCAC TCCGGCAGCA CCTTGACTCT GCACTTCAGG CCAGCAGAGT ACATGTTTAT
    ATGTACAACA GACAATGGAA GTTGGAACAT TTATGTTATA AATCAGGAGA ACTCATCACA
    GAAGCAGGCT ATATGGACCA GATTATAGAA TATCTTTATC CTTGCTTAAT TATCACTCCT
    TTGGACTGCT TCTGGGAGGG AGCAAAGCTA CAGTCTGGAA CTGCCTATCT ATTAGGGAAG
    CCTCCTTTGC AGTGGATCAA CTTTGACCCC TTAGAATTCT TGGAAGAGTT AAAGAAAATA
    AACTACCAAG TGGAGAGCTG GGAAGAAATG CTGAATAAAG CAGAGGTTGG TCATGGTTAT
    ATGGATCGAC CTTGCCTGAA TCCTGCTGAT CCTGATTGCC CAATCACAGC TCCCAATAAA
    AATTCTACCA AGCCTCTTGA TGTAGCCCTT GTTTTGAGTG GTGGATGCTA TGGACTGTCA
    AGAAAATACA TGCATTGGCA GGAAGAGTTA ATTATAGGTG GTACAGTCAA GAACAGTTCT
    GGTAAACTTG TCAGTGCCCA GGCTTTGCAA ACCATGTTTC AGTTAATGAC TCCTAAGCAA
    ATGTATGAGC ACTTCAAGGG ATATGAATAT GTTTCACATA TCAACTGGAA TGAGGATAAA
    GCAGCAGCAA TTCTGGAAGC CTGGCAGAGG ATGTATGTCG AGGTTGTTCA TCAAAGTGTT
    GCACAAAACT CTACTCAGAA GGTGCTTTCC TTTACTACAA CTACCCTGGA CGACATCCTA
    AAGTCATTTT CTGATGTCAG TGTTATCAGA GTAGCTAGTG GCTACTTACT AATGCTTGCC
    TACGCCTGTT TAACAATGCT GAGGTGGGAT TGTGCCAAGT CTCAAGGTGC CGTGGGGCTG
    GCAGGAGTCT TGTTGGTTGC ACTGTCAGTG GCTGCAGGCT TGGGTCTGTG CTCACTGATT
    GGAATTTCCT TTAATGCTGC CACAACTCAG GTTCTGCCTT TTCTTGCTCT CGGAGTTGGT
    GTAGATGATG TTTTCCTTCT AGCCCATGCG TTTAGTGAAA CAGGACAGAA TAAGAGAATT
    CCATTCGAGG ACAGAACAGG TGAATGTTTA AAGCGAACAG GAGCAAGTGT AGCCCTTACA
    TCTATCAGCA ATGTTACTGC ATTCTTTATG GCTGCCTTAA TCCCTATTCC TGCTTTACGA
    GCATTTTCAC TCCAAGCAGC AGTTGTGGTA GTATTCAACT TTGCAATGGT TCTGCTAATT
    TTTCCTGCTA TACTGAGCAT GGACCTTTAT CGTCGAGAAG ACAGGAGACT GGATATATTC
    TGCTGTTTTA CAAGTCCATG TGTCACCAGA GTGATTCAGA TCGAGCCTCA AGCGTATGCA
    GAAAATGACA ATATTTGTTA CAGTTCTCCA CCCCCGTACA GCAGTCACAG TTTTGCACAT
    GAAACTCAGA TTACAATGCA GTCTACAGTG CAGTTGCGCA CTGAATATGA TCCTCATACA
    CAAGCGTACT ACACCACTGC AGAGCCACGA TCAGAAATAT CAGTACAGCC TGTGACAGTG
    ACTCAAGATA GTCTTAGCTG CCAAAGCCCA GAAAGCGCAA GCTCAACAAG GGATTTGCTT
    TCCCAGTTCT CAGACTCCAG TGTGCATTGC CTTGAGCCAC CCTGTACGAA GTGGACACTC
    TCAACTTTTG CAGAAAAACA TTATGCTCCG TTCCTCCTAA AACCAAAAGC TAAGGTTGTG
    GTTATTTTTC TTTTCCTGGG CTTACTTGGG CTCAGCCTCT ATGGAACTAC CCGGGTGAGA
    GATGGACTAG ATCTCACAGA CATTGTACCA CGGGATACAC GGGAATATGA CTTTATTGCT
    GCGCAGTTCA AATACTTCTC ATTCTACAAT ATGTACATTG TGACACAGAA AGCAGACTAT
    CCAAATGTCC AGCACTTACT TTACGAACTT CACAGAAGTT TCAGCAATGT GACATATGTT
    TTGTTGGAAG GAGATAGGCA GCTTCCCAAA ATGTGGTTAC ATTACTTTCG AGACTGGCTG
    CAAGGACTCC AGGATGCATT TGACAGTGAC TGGGAAACTG GGAAAATCAC TTACAGCAAC
    TATAAAAATG GATCTGATGA TGCTGTTCTG GCTTATAAAC TCTTGGTACA AACAGGCAAC
    CGAGCAAAGC CCATTGACAT CAGTCAGTTG ACTAAACAGC GTCTGGTGGA TGCAGATGGA
    ATCATTAACC CAAATGCTTT CTACATCTAC CTGACAGCCT GGGTTAGCAA TGACCCTGTG
    GCCTATGCTG CCTCTCAAGC CAACATCCGG CCTCACCGCC CAGAATGGGT GCATGACAAA
    GCAGACTATA TGCCAGAAAC AAGGCTAAGA ATTCCAGCGG CTGAGCCCAT AGAATATGCT
    CAGTTTCCTT TCTACCTCAA TGGATTACGT GAGACTTCTG ACTTTGTGGA AGCTATTGAG
    AAAGTGAGAG CTATCTGCAA TAACTACACA AGCCTCGGGA TCGCTAGCTA CCCAAATGGT
    TACCCATTTC TATTCTGGGA GCAGTACATT GGGCTTCGTC ATTGGCTCCT CTTATCCATT
    AGCGTGGTTT TGGCTTGCAC GTTTCTGGTG TGTGCCCTCT TCCTCCTAAA CCCCTGGACA
    GCTGGGATCA TTGTGGTGGT GCTGGCTCTG ATGACTGTGG AGCTGTTTGG CATGATGGGT
    TTAATTGGAA TAAAACTAAG TGCAGTGCCT GTGGTTATCC TGATTGCTTC TGTTGGAATT
    GGTGTGGAAT TCACTGTTCA CATTGCTTTG GCATTTTTAA CTGCCATAGG AGATAAGAAC
    CGTAGGGCTG TCCTTGCATT GGAACATATG TTTGCACCAG TGTTGGATGG AGCTGTGTCC
    ACTCTTCTTG GAGTGTTAAT GCTTGCAGGA TCAGAGTTTG ATTTTATTGT CAGGTATTTC
    TTTGCTGTTT TGGCAATCTT AACCATTCTG GGAGTTCTCA ATGGATTGGT GCTGCTTCCT
    GTTCTTCTTT CTTTCTTTGG ACCATACCCT GAGGTTTCTC CAGCCTGTGG TCGAAACAGA
    TTACCTACAC CATCTCCTGA GCCACCTCCG AGTATTGTGA GGTTTGCACT GCCGCCTGGG
    CACACAAATA ATGGGTCAGA TTCGTCTGAT TCAGAGTACA GCTCTCAAAC CACCGTGTCA
    GGCATCAGTG AGGAGCTCCA TCATTATGAG GCCACCCAAA GCCCTGGGAT ACCAGTCCAT
    CAAGTAGTAG TGGAAGCCAC TGAGAATCCT GTCTTTGCAA GATCCACTGT GGTACAGCCA
    GAGTCAAGGC ATCAGTCAAG TCCCAGATTG CAATCAAATC CAGAGGCTGG CACACAGCAG
    GTGTGGCATC AGGGCAGACA ACCCAAGCAG GAAGTGAGGG AAGGACTGCG ACCACCTCCT
    TACCGCCCTC GCAGGGACGC TTTTGAAATT TCTACTGAAG GGCATTCAGG ACCTAGCAAT
    AAGGATCGCT TGAACCACAA GGCTCATTCT CATAACATGA GAAGCCCAGC ATTTGGTGCC
    ATGGGTGTTC CAGGATCTGC CTACTGCCAG CCTATCACTA CTGTGACTGC TTCGGCTTCA
    GTGACTGTTG CTGTCCACCC TGCTGTGCAC AGCCACAACT CTTGCAGAGG GAGCTTTCCA
    TCCTGTGAAG AATACAATGA AGATGACCGT GAGATGTTTG AGGACCCCCA TGTGCCTTTT
    AATGTGCGGT GTGAAAGAAG AAATTCTAAA GTAGAAGTTA TAGAGCTTCA GGATGTCGAA
    TGTGAGGAGA GGACAGCTGG CAAAATCTCA GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48039
    Clone ID Related Accession (Same CDS sequence) XM_015280248.2 , XM_015280248.1
    Accession Version XM_015280248.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4182bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein patched homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015280248.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGGTCGC ACCCGGCCCC GTCAAGAGGC TCCCCGCCGG CTCCGGCAGC CATGGGGAGA 
    GCTACCGGGA GGAGAGCGCC GCTGTGGCTG CGGGCGAAGT TTCAGAGACT GCTCTTTAAC
    CTGGGCTGCT ACATTCAGAA AAACTGCGGC AAGTTCCTGG TGGTCGGCCT GCTGATATTC
    GGCGCGTTCG CGGTGGGACT ACGGGCGGCC AACCTAGAGA CCAACGTAGA GGAGCTGTGG
    GTGGAAGTTG GTGGACGAGT GAGTCGGGAG TTAAACTACA CACGGCAGAA GATTGGAGAA
    GAGGCCATGT TTAACCCCCA GCTCATGATT CAGACTCCAC AGGAAGATGG AACTAATGTA
    CTCACAACAG AAGCACTCCG GCAGCACCTT GACTCTGCAC TTCAGGCCAG CAGAGTACAT
    GTTTATATGT ACAACAGACA ATGGAAGTTG GAACATTTAT GTTATAAATC AGGAGAACTC
    ATCACAGAAG CAGGCTATAT GGACCAGATT ATAGAATATC TTTATCCTTG CTTAATTATC
    ACTCCTTTGG ACTGCTTCTG GGAGGGAGCA AAGCTACAGT CTGGAACTGC CTATCTATTA
    GGGAAGCCTC CTTTGCAGTG GATCAACTTT GACCCCTTAG AATTCTTGGA AGAGTTAAAG
    AAAATAAACT ACCAAGTGGA GAGCTGGGAA GAAATGCTGA ATAAAGCAGA GGTTGGTCAT
    GGTTATATGG ATCGACCTTG CCTGAATCCT GCTGATCCTG ATTGCCCAAT CACAGCTCCC
    AATAAAAATT CTACCAAGCC TCTTGATGTA GCCCTTGTTT TGAGTGGTGG ATGCTATGGA
    CTGTCAAGAA AATACATGCA TTGGCAGGAA GAGTTAATTA TAGGTGGTAC AGTCAAGAAC
    AGTTCTGGTA AACTTGTCAG TGCCCAGGCT TTGCAAACCA TGTTTCAGTT AATGACTCCT
    AAGCAAATGT ATGAGCACTT CAAGGGATAT GAATATGTTT CACATATCAA CTGGAATGAG
    GATAAAGCAG CAGCAATTCT GGAAGCCTGG CAGAGGATGT ATGTCGAGGT TGTTCATCAA
    AGTGTTGCAC AAAACTCTAC TCAGAAGGTG CTTTCCTTTA CTACAACTAC CCTGGACGAC
    ATCCTAAAGT CATTTTCTGA TGTCAGTGTT ATCAGAGTAG CTAGTGGCTA CTTACTAATG
    CTTGCCTACG CCTGTTTAAC AATGCTGAGG TGGGATTGTG CCAAGTCTCA AGGTGCCGTG
    GGGCTGGCAG GAGTCTTGTT GGTTGCACTG TCAGTGGCTG CAGGCTTGGG TCTGTGCTCA
    CTGATTGGAA TTTCCTTTAA TGCTGCCACA ACTCAGGTTC TGCCTTTTCT TGCTCTCGGA
    GTTGGTGTAG ATGATGTTTT CCTTCTAGCC CATGCGTTTA GTGAAACAGG ACAGAATAAG
    AGAATTCCAT TCGAGGACAG AACAGGTGAA TGTTTAAAGC GAACAGGAGC AAGTGTAGCC
    CTTACATCTA TCAGCAATGT TACTGCATTC TTTATGGCTG CCTTAATCCC TATTCCTGCT
    TTACGAGCAT TTTCACTCCA AGCAGCAGTT GTGGTAGTAT TCAACTTTGC AATGGTTCTG
    CTAATTTTTC CTGCTATACT GAGCATGGAC CTTTATCGTC GAGAAGACAG GAGACTGGAT
    ATATTCTGCT GTTTTACAAG TCCATGTGTC ACCAGAGTGA TTCAGATCGA GCCTCAAGCG
    TATGCAGAAA ATGACAATAT TTGTTACAGT TCTCCACCCC CGTACAGCAG TCACAGTTTT
    GCACATGAAA CTCAGATTAC AATGCAGTCT ACAGTGCAGT TGCGCACTGA ATATGATCCT
    CATACACAAG CGTACTACAC CACTGCAGAG CCACGATCAG AAATATCAGT ACAGCCTGTG
    ACAGTGACTC AAGATAGTCT TAGCTGCCAA AGCCCAGAAA GCGCAAGCTC AACAAGGGAT
    TTGCTTTCCC AGTTCTCAGA CTCCAGTGTG CATTGCCTTG AGCCACCCTG TACGAAGTGG
    ACACTCTCAA CTTTTGCAGA AAAACATTAT GCTCCGTTCC TCCTAAAACC AAAAGCTAAG
    GTTGTGGTTA TTTTTCTTTT CCTGGGCTTA CTTGGGCTCA GCCTCTATGG AACTACCCGG
    GTGAGAGATG GACTAGATCT CACAGACATT GTACCACGGG ATACACGGGA ATATGACTTT
    ATTGCTGCGC AGTTCAAATA CTTCTCATTC TACAATATGT ACATTGTGAC ACAGAAAGCA
    GACTATCCAA ATGTCCAGCA CTTACTTTAC GAACTTCACA GAAGTTTCAG CAATGTGACA
    TATGTTTTGT TGGAAGGAGA TAGGCAGCTT CCCAAAATGT GGTTACATTA CTTTCGAGAC
    TGGCTGCAAG GACTCCAGGA TGCATTTGAC AGTGACTGGG AAACTGGGAA AATCACTTAC
    AGCAACTATA AAAATGGATC TGATGATGCT GTTCTGGCTT ATAAACTCTT GGTACAAACA
    GGCAACCGAG CAAAGCCCAT TGACATCAGT CAGTTGACTA AACAGCGTCT GGTGGATGCA
    GATGGAATCA TTAACCCAAA TGCTTTCTAC ATCTACCTGA CAGCCTGGGT TAGCAATGAC
    CCTGTGGCCT ATGCTGCCTC TCAAGCCAAC ATCCGGCCTC ACCGCCCAGA ATGGGTGCAT
    GACAAAGCAG ACTATATGCC AGAAACAAGG CTAAGAATTC CAGCGGCTGA GCCCATAGAA
    TATGCTCAGT TTCCTTTCTA CCTCAATGGA TTACGTGAGA CTTCTGACTT TGTGGAAGCT
    ATTGAGAAAG TGAGAGCTAT CTGCAATAAC TACACAAGCC TCGGGATCGC TAGCTACCCA
    AATGGTTACC CATTTCTATT CTGGGAGCAG TACATTGGGC TTCGTCATTG GCTCCTCTTA
    TCCATTAGCG TGGTTTTGGC TTGCACGTTT CTGGTGTGTG CCCTCTTCCT CCTAAACCCC
    TGGACAGCTG GGATCATTGT GGTGGTGCTG GCTCTGATGA CTGTGGAGCT GTTTGGCATG
    ATGGGTTTAA TTGGAATAAA ACTAAGTGCA GTGCCTGTGG TTATCCTGAT TGCTTCTGTT
    GGAATTGGTG TGGAATTCAC TGTTCACATT GCTTTGGCAT TTTTAACTGC CATAGGAGAT
    AAGAACCGTA GGGCTGTCCT TGCATTGGAA CATATGTTTG CACCAGTGTT GGATGGAGCT
    GTGTCCACTC TTCTTGGAGT GTTAATGCTT GCAGGATCAG AGTTTGATTT TATTGTCAGG
    TATTTCTTTG CTGTTTTGGC AATCTTAACC ATTCTGGGAG TTCTCAATGG ATTGGTGCTG
    CTTCCTGTTC TTCTTTCTTT CTTTGGACCA TACCCTGAGG TTTCTCCAGC CTGTGGTCGA
    AACAGATTAC CTACACCATC TCCTGAGCCA CCTCCGAGTA TTGTGAGGTT TGCACTGCCG
    CCTGGGCACA CAAATAATGG GTCAGATTCG TCTGATTCAG AGTACAGCTC TCAAACCACC
    GTGTCAGGCA TCAGTGAGGA GCTCCATCAT TATGAGGCCA CCCAAAGCCC TGGGATACCA
    GTCCATCAAG TAGTAGTGGA AGCCACTGAG AATCCTGTCT TTGCAAGATC CACTGTGGTA
    CAGCCAGAGT CAAGGCATCA GTCAAGTCCC AGATTGCAAT CAAATCCAGA GGCTGGCACA
    CAGCAGGTGT GGCATCAGGG CAGACAACCC AAGCAGGAAG TGAGGGAAGG ACTGCGACCA
    CCTCCTTACC GCCCTCGCAG GGACGCTTTT GAAATTTCTA CTGAAGGGCA TTCAGGACCT
    AGCAATAAGG ATCGCTTGAA CCACAAGGCT CATTCTCATA ACATGAGAAG CCCAGCATTT
    GGTGCCATGG GTGTTCCAGG ATCTGCCTAC TGCCAGCCTA TCACTACTGT GACTGCTTCG
    GCTTCAGTGA CTGTTGCTGT CCACCCTGCT GTGCACAGCC ACAACTCTTG CAGAGGGAGC
    TTTCCATCCT GTGAAGAATA CAATGAAGAT GACCGTGAGA TGTTTGAGGA CCCCCATGTG
    CCTTTTAATG TGCGGTGTGA AAGAAGAAAT TCTAAAGTAG AAGTTATAGA GCTTCAGGAT
    GTCGAATGTG AGGAGAGGAC AGCTGGCAAA ATCTCAGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015135734.1
    CDS138..4319
    Translation

    Target ORF information:

    RefSeq Version XM_015280248.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus patched 1 (PTCH1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280248.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGGTCGC ACCCGGCCCC GTCAAGAGGC TCCCCGCCGG CTCCGGCAGC CATGGGGAGA 
    GCTACCGGGA GGAGAGCGCC GCTGTGGCTG CGGGCGAAGT TTCAGAGACT GCTCTTTAAC
    CTGGGCTGCT ACATTCAGAA AAACTGCGGC AAGTTCCTGG TGGTCGGCCT GCTGATATTC
    GGCGCGTTCG CGGTGGGACT ACGGGCGGCC AACCTAGAGA CCAACGTAGA GGAGCTGTGG
    GTGGAAGTTG GTGGACGAGT GAGTCGGGAG TTAAACTACA CACGGCAGAA GATTGGAGAA
    GAGGCCATGT TTAACCCCCA GCTCATGATT CAGACTCCAC AGGAAGATGG AACTAATGTA
    CTCACAACAG AAGCACTCCG GCAGCACCTT GACTCTGCAC TTCAGGCCAG CAGAGTACAT
    GTTTATATGT ACAACAGACA ATGGAAGTTG GAACATTTAT GTTATAAATC AGGAGAACTC
    ATCACAGAAG CAGGCTATAT GGACCAGATT ATAGAATATC TTTATCCTTG CTTAATTATC
    ACTCCTTTGG ACTGCTTCTG GGAGGGAGCA AAGCTACAGT CTGGAACTGC CTATCTATTA
    GGGAAGCCTC CTTTGCAGTG GATCAACTTT GACCCCTTAG AATTCTTGGA AGAGTTAAAG
    AAAATAAACT ACCAAGTGGA GAGCTGGGAA GAAATGCTGA ATAAAGCAGA GGTTGGTCAT
    GGTTATATGG ATCGACCTTG CCTGAATCCT GCTGATCCTG ATTGCCCAAT CACAGCTCCC
    AATAAAAATT CTACCAAGCC TCTTGATGTA GCCCTTGTTT TGAGTGGTGG ATGCTATGGA
    CTGTCAAGAA AATACATGCA TTGGCAGGAA GAGTTAATTA TAGGTGGTAC AGTCAAGAAC
    AGTTCTGGTA AACTTGTCAG TGCCCAGGCT TTGCAAACCA TGTTTCAGTT AATGACTCCT
    AAGCAAATGT ATGAGCACTT CAAGGGATAT GAATATGTTT CACATATCAA CTGGAATGAG
    GATAAAGCAG CAGCAATTCT GGAAGCCTGG CAGAGGATGT ATGTCGAGGT TGTTCATCAA
    AGTGTTGCAC AAAACTCTAC TCAGAAGGTG CTTTCCTTTA CTACAACTAC CCTGGACGAC
    ATCCTAAAGT CATTTTCTGA TGTCAGTGTT ATCAGAGTAG CTAGTGGCTA CTTACTAATG
    CTTGCCTACG CCTGTTTAAC AATGCTGAGG TGGGATTGTG CCAAGTCTCA AGGTGCCGTG
    GGGCTGGCAG GAGTCTTGTT GGTTGCACTG TCAGTGGCTG CAGGCTTGGG TCTGTGCTCA
    CTGATTGGAA TTTCCTTTAA TGCTGCCACA ACTCAGGTTC TGCCTTTTCT TGCTCTCGGA
    GTTGGTGTAG ATGATGTTTT CCTTCTAGCC CATGCGTTTA GTGAAACAGG ACAGAATAAG
    AGAATTCCAT TCGAGGACAG AACAGGTGAA TGTTTAAAGC GAACAGGAGC AAGTGTAGCC
    CTTACATCTA TCAGCAATGT TACTGCATTC TTTATGGCTG CCTTAATCCC TATTCCTGCT
    TTACGAGCAT TTTCACTCCA AGCAGCAGTT GTGGTAGTAT TCAACTTTGC AATGGTTCTG
    CTAATTTTTC CTGCTATACT GAGCATGGAC CTTTATCGTC GAGAAGACAG GAGACTGGAT
    ATATTCTGCT GTTTTACAAG TCCATGTGTC ACCAGAGTGA TTCAGATCGA GCCTCAAGCG
    TATGCAGAAA ATGACAATAT TTGTTACAGT TCTCCACCCC CGTACAGCAG TCACAGTTTT
    GCACATGAAA CTCAGATTAC AATGCAGTCT ACAGTGCAGT TGCGCACTGA ATATGATCCT
    CATACACAAG CGTACTACAC CACTGCAGAG CCACGATCAG AAATATCAGT ACAGCCTGTG
    ACAGTGACTC AAGATAGTCT TAGCTGCCAA AGCCCAGAAA GCGCAAGCTC AACAAGGGAT
    TTGCTTTCCC AGTTCTCAGA CTCCAGTGTG CATTGCCTTG AGCCACCCTG TACGAAGTGG
    ACACTCTCAA CTTTTGCAGA AAAACATTAT GCTCCGTTCC TCCTAAAACC AAAAGCTAAG
    GTTGTGGTTA TTTTTCTTTT CCTGGGCTTA CTTGGGCTCA GCCTCTATGG AACTACCCGG
    GTGAGAGATG GACTAGATCT CACAGACATT GTACCACGGG ATACACGGGA ATATGACTTT
    ATTGCTGCGC AGTTCAAATA CTTCTCATTC TACAATATGT ACATTGTGAC ACAGAAAGCA
    GACTATCCAA ATGTCCAGCA CTTACTTTAC GAACTTCACA GAAGTTTCAG CAATGTGACA
    TATGTTTTGT TGGAAGGAGA TAGGCAGCTT CCCAAAATGT GGTTACATTA CTTTCGAGAC
    TGGCTGCAAG GACTCCAGGA TGCATTTGAC AGTGACTGGG AAACTGGGAA AATCACTTAC
    AGCAACTATA AAAATGGATC TGATGATGCT GTTCTGGCTT ATAAACTCTT GGTACAAACA
    GGCAACCGAG CAAAGCCCAT TGACATCAGT CAGTTGACTA AACAGCGTCT GGTGGATGCA
    GATGGAATCA TTAACCCAAA TGCTTTCTAC ATCTACCTGA CAGCCTGGGT TAGCAATGAC
    CCTGTGGCCT ATGCTGCCTC TCAAGCCAAC ATCCGGCCTC ACCGCCCAGA ATGGGTGCAT
    GACAAAGCAG ACTATATGCC AGAAACAAGG CTAAGAATTC CAGCGGCTGA GCCCATAGAA
    TATGCTCAGT TTCCTTTCTA CCTCAATGGA TTACGTGAGA CTTCTGACTT TGTGGAAGCT
    ATTGAGAAAG TGAGAGCTAT CTGCAATAAC TACACAAGCC TCGGGATCGC TAGCTACCCA
    AATGGTTACC CATTTCTATT CTGGGAGCAG TACATTGGGC TTCGTCATTG GCTCCTCTTA
    TCCATTAGCG TGGTTTTGGC TTGCACGTTT CTGGTGTGTG CCCTCTTCCT CCTAAACCCC
    TGGACAGCTG GGATCATTGT GGTGGTGCTG GCTCTGATGA CTGTGGAGCT GTTTGGCATG
    ATGGGTTTAA TTGGAATAAA ACTAAGTGCA GTGCCTGTGG TTATCCTGAT TGCTTCTGTT
    GGAATTGGTG TGGAATTCAC TGTTCACATT GCTTTGGCAT TTTTAACTGC CATAGGAGAT
    AAGAACCGTA GGGCTGTCCT TGCATTGGAA CATATGTTTG CACCAGTGTT GGATGGAGCT
    GTGTCCACTC TTCTTGGAGT GTTAATGCTT GCAGGATCAG AGTTTGATTT TATTGTCAGG
    TATTTCTTTG CTGTTTTGGC AATCTTAACC ATTCTGGGAG TTCTCAATGG ATTGGTGCTG
    CTTCCTGTTC TTCTTTCTTT CTTTGGACCA TACCCTGAGG TTTCTCCAGC CTGTGGTCGA
    AACAGATTAC CTACACCATC TCCTGAGCCA CCTCCGAGTA TTGTGAGGTT TGCACTGCCG
    CCTGGGCACA CAAATAATGG GTCAGATTCG TCTGATTCAG AGTACAGCTC TCAAACCACC
    GTGTCAGGCA TCAGTGAGGA GCTCCATCAT TATGAGGCCA CCCAAAGCCC TGGGATACCA
    GTCCATCAAG TAGTAGTGGA AGCCACTGAG AATCCTGTCT TTGCAAGATC CACTGTGGTA
    CAGCCAGAGT CAAGGCATCA GTCAAGTCCC AGATTGCAAT CAAATCCAGA GGCTGGCACA
    CAGCAGGTGT GGCATCAGGG CAGACAACCC AAGCAGGAAG TGAGGGAAGG ACTGCGACCA
    CCTCCTTACC GCCCTCGCAG GGACGCTTTT GAAATTTCTA CTGAAGGGCA TTCAGGACCT
    AGCAATAAGG ATCGCTTGAA CCACAAGGCT CATTCTCATA ACATGAGAAG CCCAGCATTT
    GGTGCCATGG GTGTTCCAGG ATCTGCCTAC TGCCAGCCTA TCACTACTGT GACTGCTTCG
    GCTTCAGTGA CTGTTGCTGT CCACCCTGCT GTGCACAGCC ACAACTCTTG CAGAGGGAGC
    TTTCCATCCT GTGAAGAATA CAATGAAGAT GACCGTGAGA TGTTTGAGGA CCCCCATGTG
    CCTTTTAATG TGCGGTGTGA AAGAAGAAAT TCTAAAGTAG AAGTTATAGA GCTTCAGGAT
    GTCGAATGTG AGGAGAGGAC AGCTGGCAAA ATCTCAGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48039
    Clone ID Related Accession (Same CDS sequence) XM_015280248.2 , XM_015280248.1
    Accession Version XM_015280248.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4182bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product protein patched homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764318.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGGTCGC ACCCGGCCCC GTCAAGAGGC TCCCCGCCGG CTCCGGCAGC CATGGGGAGA 
    GCTACCGGGA GGAGAGCGCC GCTGTGGCTG CGGGCGAAGT TTCAGAGACT GCTCTTTAAC
    CTGGGCTGCT ACATTCAGAA AAACTGCGGC AAGTTCCTGG TGGTCGGCCT GCTGATATTC
    GGCGCGTTCG CGGTGGGACT ACGGGCGGCC AACCTAGAGA CCAACGTAGA GGAGCTGTGG
    GTGGAAGTTG GTGGACGAGT GAGTCGGGAG TTAAACTACA CACGGCAGAA GATTGGAGAA
    GAGGCCATGT TTAACCCCCA GCTCATGATT CAGACTCCAC AGGAAGATGG AACTAATGTA
    CTCACAACAG AAGCACTCCG GCAGCACCTT GACTCTGCAC TTCAGGCCAG CAGAGTACAT
    GTTTATATGT ACAACAGACA ATGGAAGTTG GAACATTTAT GTTATAAATC AGGAGAACTC
    ATCACAGAAG CAGGCTATAT GGACCAGATT ATAGAATATC TTTATCCTTG CTTAATTATC
    ACTCCTTTGG ACTGCTTCTG GGAGGGAGCA AAGCTACAGT CTGGAACTGC CTATCTATTA
    GGGAAGCCTC CTTTGCAGTG GATCAACTTT GACCCCTTAG AATTCTTGGA AGAGTTAAAG
    AAAATAAACT ACCAAGTGGA GAGCTGGGAA GAAATGCTGA ATAAAGCAGA GGTTGGTCAT
    GGTTATATGG ATCGACCTTG CCTGAATCCT GCTGATCCTG ATTGCCCAAT CACAGCTCCC
    AATAAAAATT CTACCAAGCC TCTTGATGTA GCCCTTGTTT TGAGTGGTGG ATGCTATGGA
    CTGTCAAGAA AATACATGCA TTGGCAGGAA GAGTTAATTA TAGGTGGTAC AGTCAAGAAC
    AGTTCTGGTA AACTTGTCAG TGCCCAGGCT TTGCAAACCA TGTTTCAGTT AATGACTCCT
    AAGCAAATGT ATGAGCACTT CAAGGGATAT GAATATGTTT CACATATCAA CTGGAATGAG
    GATAAAGCAG CAGCAATTCT GGAAGCCTGG CAGAGGATGT ATGTCGAGGT TGTTCATCAA
    AGTGTTGCAC AAAACTCTAC TCAGAAGGTG CTTTCCTTTA CTACAACTAC CCTGGACGAC
    ATCCTAAAGT CATTTTCTGA TGTCAGTGTT ATCAGAGTAG CTAGTGGCTA CTTACTAATG
    CTTGCCTACG CCTGTTTAAC AATGCTGAGG TGGGATTGTG CCAAGTCTCA AGGTGCCGTG
    GGGCTGGCAG GAGTCTTGTT GGTTGCACTG TCAGTGGCTG CAGGCTTGGG TCTGTGCTCA
    CTGATTGGAA TTTCCTTTAA TGCTGCCACA ACTCAGGTTC TGCCTTTTCT TGCTCTCGGA
    GTTGGTGTAG ATGATGTTTT CCTTCTAGCC CATGCGTTTA GTGAAACAGG ACAGAATAAG
    AGAATTCCAT TCGAGGACAG AACAGGTGAA TGTTTAAAGC GAACAGGAGC AAGTGTAGCC
    CTTACATCTA TCAGCAATGT TACTGCATTC TTTATGGCTG CCTTAATCCC TATTCCTGCT
    TTACGAGCAT TTTCACTCCA AGCAGCAGTT GTGGTAGTAT TCAACTTTGC AATGGTTCTG
    CTAATTTTTC CTGCTATACT GAGCATGGAC CTTTATCGTC GAGAAGACAG GAGACTGGAT
    ATATTCTGCT GTTTTACAAG TCCATGTGTC ACCAGAGTGA TTCAGATCGA GCCTCAAGCG
    TATGCAGAAA ATGACAATAT TTGTTACAGT TCTCCACCCC CGTACAGCAG TCACAGTTTT
    GCACATGAAA CTCAGATTAC AATGCAGTCT ACAGTGCAGT TGCGCACTGA ATATGATCCT
    CATACACAAG CGTACTACAC CACTGCAGAG CCACGATCAG AAATATCAGT ACAGCCTGTG
    ACAGTGACTC AAGATAGTCT TAGCTGCCAA AGCCCAGAAA GCGCAAGCTC AACAAGGGAT
    TTGCTTTCCC AGTTCTCAGA CTCCAGTGTG CATTGCCTTG AGCCACCCTG TACGAAGTGG
    ACACTCTCAA CTTTTGCAGA AAAACATTAT GCTCCGTTCC TCCTAAAACC AAAAGCTAAG
    GTTGTGGTTA TTTTTCTTTT CCTGGGCTTA CTTGGGCTCA GCCTCTATGG AACTACCCGG
    GTGAGAGATG GACTAGATCT CACAGACATT GTACCACGGG ATACACGGGA ATATGACTTT
    ATTGCTGCGC AGTTCAAATA CTTCTCATTC TACAATATGT ACATTGTGAC ACAGAAAGCA
    GACTATCCAA ATGTCCAGCA CTTACTTTAC GAACTTCACA GAAGTTTCAG CAATGTGACA
    TATGTTTTGT TGGAAGGAGA TAGGCAGCTT CCCAAAATGT GGTTACATTA CTTTCGAGAC
    TGGCTGCAAG GACTCCAGGA TGCATTTGAC AGTGACTGGG AAACTGGGAA AATCACTTAC
    AGCAACTATA AAAATGGATC TGATGATGCT GTTCTGGCTT ATAAACTCTT GGTACAAACA
    GGCAACCGAG CAAAGCCCAT TGACATCAGT CAGTTGACTA AACAGCGTCT GGTGGATGCA
    GATGGAATCA TTAACCCAAA TGCTTTCTAC ATCTACCTGA CAGCCTGGGT TAGCAATGAC
    CCTGTGGCCT ATGCTGCCTC TCAAGCCAAC ATCCGGCCTC ACCGCCCAGA ATGGGTGCAT
    GACAAAGCAG ACTATATGCC AGAAACAAGG CTAAGAATTC CAGCGGCTGA GCCCATAGAA
    TATGCTCAGT TTCCTTTCTA CCTCAATGGA TTACGTGAGA CTTCTGACTT TGTGGAAGCT
    ATTGAGAAAG TGAGAGCTAT CTGCAATAAC TACACAAGCC TCGGGATCGC TAGCTACCCA
    AATGGTTACC CATTTCTATT CTGGGAGCAG TACATTGGGC TTCGTCATTG GCTCCTCTTA
    TCCATTAGCG TGGTTTTGGC TTGCACGTTT CTGGTGTGTG CCCTCTTCCT CCTAAACCCC
    TGGACAGCTG GGATCATTGT GGTGGTGCTG GCTCTGATGA CTGTGGAGCT GTTTGGCATG
    ATGGGTTTAA TTGGAATAAA ACTAAGTGCA GTGCCTGTGG TTATCCTGAT TGCTTCTGTT
    GGAATTGGTG TGGAATTCAC TGTTCACATT GCTTTGGCAT TTTTAACTGC CATAGGAGAT
    AAGAACCGTA GGGCTGTCCT TGCATTGGAA CATATGTTTG CACCAGTGTT GGATGGAGCT
    GTGTCCACTC TTCTTGGAGT GTTAATGCTT GCAGGATCAG AGTTTGATTT TATTGTCAGG
    TATTTCTTTG CTGTTTTGGC AATCTTAACC ATTCTGGGAG TTCTCAATGG ATTGGTGCTG
    CTTCCTGTTC TTCTTTCTTT CTTTGGACCA TACCCTGAGG TTTCTCCAGC CTGTGGTCGA
    AACAGATTAC CTACACCATC TCCTGAGCCA CCTCCGAGTA TTGTGAGGTT TGCACTGCCG
    CCTGGGCACA CAAATAATGG GTCAGATTCG TCTGATTCAG AGTACAGCTC TCAAACCACC
    GTGTCAGGCA TCAGTGAGGA GCTCCATCAT TATGAGGCCA CCCAAAGCCC TGGGATACCA
    GTCCATCAAG TAGTAGTGGA AGCCACTGAG AATCCTGTCT TTGCAAGATC CACTGTGGTA
    CAGCCAGAGT CAAGGCATCA GTCAAGTCCC AGATTGCAAT CAAATCCAGA GGCTGGCACA
    CAGCAGGTGT GGCATCAGGG CAGACAACCC AAGCAGGAAG TGAGGGAAGG ACTGCGACCA
    CCTCCTTACC GCCCTCGCAG GGACGCTTTT GAAATTTCTA CTGAAGGGCA TTCAGGACCT
    AGCAATAAGG ATCGCTTGAA CCACAAGGCT CATTCTCATA ACATGAGAAG CCCAGCATTT
    GGTGCCATGG GTGTTCCAGG ATCTGCCTAC TGCCAGCCTA TCACTACTGT GACTGCTTCG
    GCTTCAGTGA CTGTTGCTGT CCACCCTGCT GTGCACAGCC ACAACTCTTG CAGAGGGAGC
    TTTCCATCCT GTGAAGAATA CAATGAAGAT GACCGTGAGA TGTTTGAGGA CCCCCATGTG
    CCTTTTAATG TGCGGTGTGA AAGAAGAAAT TCTAAAGTAG AAGTTATAGA GCTTCAGGAT
    GTCGAATGTG AGGAGAGGAC AGCTGGCAAA ATCTCAGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015135734.1
    CDS113..4294
    Translation

    Target ORF information:

    RefSeq Version XM_015280248.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus patched 1 (PTCH1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280248.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGGTCGC ACCCGGCCCC GTCAAGAGGC TCCCCGCCGG CTCCGGCAGC CATGGGGAGA 
    GCTACCGGGA GGAGAGCGCC GCTGTGGCTG CGGGCGAAGT TTCAGAGACT GCTCTTTAAC
    CTGGGCTGCT ACATTCAGAA AAACTGCGGC AAGTTCCTGG TGGTCGGCCT GCTGATATTC
    GGCGCGTTCG CGGTGGGACT ACGGGCGGCC AACCTAGAGA CCAACGTAGA GGAGCTGTGG
    GTGGAAGTTG GTGGACGAGT GAGTCGGGAG TTAAACTACA CACGGCAGAA GATTGGAGAA
    GAGGCCATGT TTAACCCCCA GCTCATGATT CAGACTCCAC AGGAAGATGG AACTAATGTA
    CTCACAACAG AAGCACTCCG GCAGCACCTT GACTCTGCAC TTCAGGCCAG CAGAGTACAT
    GTTTATATGT ACAACAGACA ATGGAAGTTG GAACATTTAT GTTATAAATC AGGAGAACTC
    ATCACAGAAG CAGGCTATAT GGACCAGATT ATAGAATATC TTTATCCTTG CTTAATTATC
    ACTCCTTTGG ACTGCTTCTG GGAGGGAGCA AAGCTACAGT CTGGAACTGC CTATCTATTA
    GGGAAGCCTC CTTTGCAGTG GATCAACTTT GACCCCTTAG AATTCTTGGA AGAGTTAAAG
    AAAATAAACT ACCAAGTGGA GAGCTGGGAA GAAATGCTGA ATAAAGCAGA GGTTGGTCAT
    GGTTATATGG ATCGACCTTG CCTGAATCCT GCTGATCCTG ATTGCCCAAT CACAGCTCCC
    AATAAAAATT CTACCAAGCC TCTTGATGTA GCCCTTGTTT TGAGTGGTGG ATGCTATGGA
    CTGTCAAGAA AATACATGCA TTGGCAGGAA GAGTTAATTA TAGGTGGTAC AGTCAAGAAC
    AGTTCTGGTA AACTTGTCAG TGCCCAGGCT TTGCAAACCA TGTTTCAGTT AATGACTCCT
    AAGCAAATGT ATGAGCACTT CAAGGGATAT GAATATGTTT CACATATCAA CTGGAATGAG
    GATAAAGCAG CAGCAATTCT GGAAGCCTGG CAGAGGATGT ATGTCGAGGT TGTTCATCAA
    AGTGTTGCAC AAAACTCTAC TCAGAAGGTG CTTTCCTTTA CTACAACTAC CCTGGACGAC
    ATCCTAAAGT CATTTTCTGA TGTCAGTGTT ATCAGAGTAG CTAGTGGCTA CTTACTAATG
    CTTGCCTACG CCTGTTTAAC AATGCTGAGG TGGGATTGTG CCAAGTCTCA AGGTGCCGTG
    GGGCTGGCAG GAGTCTTGTT GGTTGCACTG TCAGTGGCTG CAGGCTTGGG TCTGTGCTCA
    CTGATTGGAA TTTCCTTTAA TGCTGCCACA ACTCAGGTTC TGCCTTTTCT TGCTCTCGGA
    GTTGGTGTAG ATGATGTTTT CCTTCTAGCC CATGCGTTTA GTGAAACAGG ACAGAATAAG
    AGAATTCCAT TCGAGGACAG AACAGGTGAA TGTTTAAAGC GAACAGGAGC AAGTGTAGCC
    CTTACATCTA TCAGCAATGT TACTGCATTC TTTATGGCTG CCTTAATCCC TATTCCTGCT
    TTACGAGCAT TTTCACTCCA AGCAGCAGTT GTGGTAGTAT TCAACTTTGC AATGGTTCTG
    CTAATTTTTC CTGCTATACT GAGCATGGAC CTTTATCGTC GAGAAGACAG GAGACTGGAT
    ATATTCTGCT GTTTTACAAG TCCATGTGTC ACCAGAGTGA TTCAGATCGA GCCTCAAGCG
    TATGCAGAAA ATGACAATAT TTGTTACAGT TCTCCACCCC CGTACAGCAG TCACAGTTTT
    GCACATGAAA CTCAGATTAC AATGCAGTCT ACAGTGCAGT TGCGCACTGA ATATGATCCT
    CATACACAAG CGTACTACAC CACTGCAGAG CCACGATCAG AAATATCAGT ACAGCCTGTG
    ACAGTGACTC AAGATAGTCT TAGCTGCCAA AGCCCAGAAA GCGCAAGCTC AACAAGGGAT
    TTGCTTTCCC AGTTCTCAGA CTCCAGTGTG CATTGCCTTG AGCCACCCTG TACGAAGTGG
    ACACTCTCAA CTTTTGCAGA AAAACATTAT GCTCCGTTCC TCCTAAAACC AAAAGCTAAG
    GTTGTGGTTA TTTTTCTTTT CCTGGGCTTA CTTGGGCTCA GCCTCTATGG AACTACCCGG
    GTGAGAGATG GACTAGATCT CACAGACATT GTACCACGGG ATACACGGGA ATATGACTTT
    ATTGCTGCGC AGTTCAAATA CTTCTCATTC TACAATATGT ACATTGTGAC ACAGAAAGCA
    GACTATCCAA ATGTCCAGCA CTTACTTTAC GAACTTCACA GAAGTTTCAG CAATGTGACA
    TATGTTTTGT TGGAAGGAGA TAGGCAGCTT CCCAAAATGT GGTTACATTA CTTTCGAGAC
    TGGCTGCAAG GACTCCAGGA TGCATTTGAC AGTGACTGGG AAACTGGGAA AATCACTTAC
    AGCAACTATA AAAATGGATC TGATGATGCT GTTCTGGCTT ATAAACTCTT GGTACAAACA
    GGCAACCGAG CAAAGCCCAT TGACATCAGT CAGTTGACTA AACAGCGTCT GGTGGATGCA
    GATGGAATCA TTAACCCAAA TGCTTTCTAC ATCTACCTGA CAGCCTGGGT TAGCAATGAC
    CCTGTGGCCT ATGCTGCCTC TCAAGCCAAC ATCCGGCCTC ACCGCCCAGA ATGGGTGCAT
    GACAAAGCAG ACTATATGCC AGAAACAAGG CTAAGAATTC CAGCGGCTGA GCCCATAGAA
    TATGCTCAGT TTCCTTTCTA CCTCAATGGA TTACGTGAGA CTTCTGACTT TGTGGAAGCT
    ATTGAGAAAG TGAGAGCTAT CTGCAATAAC TACACAAGCC TCGGGATCGC TAGCTACCCA
    AATGGTTACC CATTTCTATT CTGGGAGCAG TACATTGGGC TTCGTCATTG GCTCCTCTTA
    TCCATTAGCG TGGTTTTGGC TTGCACGTTT CTGGTGTGTG CCCTCTTCCT CCTAAACCCC
    TGGACAGCTG GGATCATTGT GGTGGTGCTG GCTCTGATGA CTGTGGAGCT GTTTGGCATG
    ATGGGTTTAA TTGGAATAAA ACTAAGTGCA GTGCCTGTGG TTATCCTGAT TGCTTCTGTT
    GGAATTGGTG TGGAATTCAC TGTTCACATT GCTTTGGCAT TTTTAACTGC CATAGGAGAT
    AAGAACCGTA GGGCTGTCCT TGCATTGGAA CATATGTTTG CACCAGTGTT GGATGGAGCT
    GTGTCCACTC TTCTTGGAGT GTTAATGCTT GCAGGATCAG AGTTTGATTT TATTGTCAGG
    TATTTCTTTG CTGTTTTGGC AATCTTAACC ATTCTGGGAG TTCTCAATGG ATTGGTGCTG
    CTTCCTGTTC TTCTTTCTTT CTTTGGACCA TACCCTGAGG TTTCTCCAGC CTGTGGTCGA
    AACAGATTAC CTACACCATC TCCTGAGCCA CCTCCGAGTA TTGTGAGGTT TGCACTGCCG
    CCTGGGCACA CAAATAATGG GTCAGATTCG TCTGATTCAG AGTACAGCTC TCAAACCACC
    GTGTCAGGCA TCAGTGAGGA GCTCCATCAT TATGAGGCCA CCCAAAGCCC TGGGATACCA
    GTCCATCAAG TAGTAGTGGA AGCCACTGAG AATCCTGTCT TTGCAAGATC CACTGTGGTA
    CAGCCAGAGT CAAGGCATCA GTCAAGTCCC AGATTGCAAT CAAATCCAGA GGCTGGCACA
    CAGCAGGTGT GGCATCAGGG CAGACAACCC AAGCAGGAAG TGAGGGAAGG ACTGCGACCA
    CCTCCTTACC GCCCTCGCAG GGACGCTTTT GAAATTTCTA CTGAAGGGCA TTCAGGACCT
    AGCAATAAGG ATCGCTTGAA CCACAAGGCT CATTCTCATA ACATGAGAAG CCCAGCATTT
    GGTGCCATGG GTGTTCCAGG ATCTGCCTAC TGCCAGCCTA TCACTACTGT GACTGCTTCG
    GCTTCAGTGA CTGTTGCTGT CCACCCTGCT GTGCACAGCC ACAACTCTTG CAGAGGGAGC
    TTTCCATCCT GTGAAGAATA CAATGAAGAT GACCGTGAGA TGTTTGAGGA CCCCCATGTG
    CCTTTTAATG TGCGGTGTGA AAGAAGAAAT TCTAAAGTAG AAGTTATAGA GCTTCAGGAT
    GTCGAATGTG AGGAGAGGAC AGCTGGCAAA ATCTCAGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa28384
    Clone ID Related Accession (Same CDS sequence) NM_204960.2
    Accession Version NM_204960.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4332bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Gallus gallus(chicken)
    Product protein patched homolog 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC186361.2. On Mar 20, 2015 this sequence version replaced NM_204960.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: U40074.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCCTCGG CCGCTGACGC CTTAGAGCCG GAGAGCGGCA GCAGCACCGC GGGCGGCGGC 
    AGCCACCCCG TTCGGGCGGC GAGGAGTGCC CGCGGCCGGC GGCGGCGGAG CGGAGGGACC
    CGGCGCGCCG CGGCGCCCGA CCGGGAGTAT CTGCAGCGGC CGAGCTACTG CGATGCGGCG
    TTCGCCTTGG AGCAGATCGC AAAGGGGAGA GCTACCGGGA GGAGAGCGCC GCTGTGGCTG
    CGGGCGAAGT TTCAGAGACT GCTCTTTAAC CTGGGCTGCT ACATTCAGAA AAACTGCGGC
    AAGTTCCTGG TGGTCGGCCT GCTGATATTC GGCGCGTTCG CGGTGGGACT ACGGGCGGCC
    AACCTAGAGA CCAACGTAGA GGAGCTGTGG GTGGAAGTTG GTGGACGAGT GAGTCGGGAG
    TTAAACTACA CACGGCAGAA GATTGGAGAA GAGGCCATGT TTAACCCCCA GCTCATGATT
    CAGACTCCAC AGGAAGATGG AACTAATGTA CTCACAACAG AAGCACTCCG GCAGCACCTT
    GACTCTGCAC TTCAGGCCAG CAGAGTACAT GTTTATATGT ACAACAGACA ATGGAAGTTG
    GAACATTTAT GTTATAAATC AGGAGAACTC ATCACAGAAG CAGGCTATAT GGACCAGATT
    ATAGAATATC TTTATCCTTG CTTAATTATC ACTCCTTTGG ACTGCTTCTG GGAGGGAGCA
    AAGCTACAGT CTGGAACTGC CTATCTATTA GGGAAGCCTC CTTTGCAGTG GATCAACTTT
    GACCCCTTAG AATTCTTGGA AGAGTTAAAG AAAATAAACT ACCAAGTGGA GAGCTGGGAA
    GAAATGCTGA ATAAAGCAGA GGTTGGTCAT GGTTATATGG ATCGACCTTG CCTGAATCCT
    GCTGATCCTG ATTGCCCAAT CACAGCTCCC AATAAAAATT CTACCAAGCC TCTTGATGTA
    GCCCTTGTTT TGAGTGGTGG ATGCTATGGA CTGTCAAGAA AATACATGCA TTGGCAGGAA
    GAGTTAATTA TAGGTGGTAC AGTCAAGAAC AGTTCTGGTA AACTTGTCAG TGCCCAGGCT
    TTGCAAACCA TGTTTCAGTT AATGACTCCT AAGCAAATGT ATGAGCACTT CAAGGGATAT
    GAATATGTTT CACATATCAA CTGGAATGAG GATAAAGCAG CAGCAATTCT GGAAGCCTGG
    CAGAGGATGT ATGTCGAGGT TGTTCATCAA AGTGTTGCAC AAAACTCTAC TCAGAAGGTG
    CTTTCCTTTA CTACAACTAC CCTGGACGAC ATCCTAAAGT CATTTTCTGA TGTCAGTGTT
    ATCAGAGTAG CTAGTGGCTA CTTACTAATG CTTGCCTACG CCTGTTTAAC AATGCTGAGG
    TGGGATTGTG CCAAGTCTCA AGGTGCCGTG GGGCTGGCAG GAGTCTTGTT GGTTGCACTG
    TCAGTGGCTG CAGGCTTGGG TCTGTGCTCA CTGATTGGAA TTTCCTTTAA TGCTGCCACA
    ACTCAGGTTC TGCCTTTTCT TGCTCTCGGA GTTGGTGTAG ATGATGTTTT CCTTCTAGCC
    CATGCGTTTA GTGAAACAGG ACAGAATAAG AGAATTCCAT TCGAGGACAG AACAGGTGAA
    TGTTTAAAGC GAACAGGAGC AAGTGTAGCC CTTACATCTA TCAGCAATGT TACTGCATTC
    TTTATGGCTG CCTTAATCCC TATTCCTGCT TTACGAGCAT TTTCACTCCA AGCAGCAGTT
    GTGGTAGTAT TCAACTTTGC AATGGTTCTG CTAATTTTTC CTGCTATACT GAGCATGGAC
    CTTTATCGTC GAGAAGACAG GAGACTGGAT ATATTCTGCT GTTTTACAAG TCCATGTGTC
    ACCAGAGTGA TTCAGATCGA GCCTCAAGCG TATGCAGAAA ATGACAATAT TTGTTACAGT
    TCTCCACCCC CGTACAGCAG TCACAGTTTT GCACATGAAA CTCAGATTAC AATGCAGTCT
    ACAGTGCAGT TGCGCACTGA ATATGATCCT CATACACAAG CGTACTACAC CACTGCAGAG
    CCACGATCAG AAATATCAGT ACAGCCTGTG ACAGTGACTC AAGATAGTCT TAGCTGCCAA
    AGCCCAGAAA GCGCAAGCTC AACAAGGGAT TTGCTTTCCC AGTTCTCAGA CTCCAGTGTG
    CATTGCCTTG AGCCACCCTG TACGAAGTGG ACACTCTCAA CTTTTGCAGA AAAACATTAT
    GCTCCGTTCC TCCTAAAACC AAAAGCTAAG GTTGTGGTTA TTTTTCTTTT CCTGGGCTTA
    CTTGGGCTCA GCCTCTATGG AACTACCCGG GTGAGAGATG GACTAGATCT CACAGACATT
    GTACCACGGG ATACACGGGA ATATGACTTT ATTGCTGCGC AGTTCAAATA CTTCTCATTC
    TACAATATGT ACATTGTGAC ACAGAAAGCA GACTATCCAA ATGTCCAGCA CTTACTTTAC
    GAACTTCACA GAAGTTTCAG CAATGTGACA TATGTTTTGT TGGAAGGAGA TAGGCAGCTT
    CCCAAAATGT GGTTACATTA CTTTCGAGAC TGGCTGCAAG GACTCCAGGA TGCATTTGAC
    AGTGACTGGG AAACTGGGAA AATCACTTAC AGCAACTATA AAAATGGATC TGATGATGCT
    GTTCTGGCTT ATAAACTCTT GGTACAAACA GGCAACCGAG CAAAGCCCAT TGACATCAGT
    CAGTTGACTA AACAGCGTCT GGTGGATGCA GATGGAATCA TTAACCCAAA TGCTTTCTAC
    ATCTACCTGA CAGCCTGGGT TAGCAATGAC CCTGTGGCCT ATGCTGCCTC TCAAGCCAAC
    ATCCGGCCTC ACCGCCCAGA ATGGGTGCAT GACAAAGCAG ACTATATGCC AGAAACAAGG
    CTAAGAATTC CAGCGGCTGA GCCCATAGAA TATGCTCAGT TTCCTTTCTA CCTCAATGGA
    TTACGTGAGA CTTCTGACTT TGTGGAAGCT ATTGAGAAAG TGAGAGCTAT CTGCAATAAC
    TACACAAGCC TCGGGATCGC TAGCTACCCA AATGGTTACC CATTTCTATT CTGGGAGCAG
    TACATTGGGC TTCGTCATTG GCTCCTCTTA TCCATTAGCG TGGTTTTGGC TTGCACGTTT
    CTGGTGTGTG CCCTCTTCCT CCTAAACCCC TGGACAGCTG GGATCATTGT GGTGGTGCTG
    GCTCTGATGA CTGTGGAGCT GTTTGGCATG ATGGGTTTAA TTGGAATAAA ACTAAGTGCA
    GTGCCTGTGG TTATCCTGAT TGCTTCTGTT GGAATTGGTG TGGAATTCAC TGTTCACATT
    GCTTTGGCAT TTTTAACTGC CATAGGAGAT AAGAACCGTA GGGCTGTCCT TGCATTGGAA
    CATATGTTTG CACCAGTGTT GGATGGAGCT GTGTCCACTC TTCTTGGAGT GTTAATGCTT
    GCAGGATCAG AGTTTGATTT TATTGTCAGG TATTTCTTTG CTGTTTTGGC AATCTTAACC
    ATTCTGGGAG TTCTCAATGG ATTGGTGCTG CTTCCTGTTC TTCTTTCTTT CTTTGGACCA
    TACCCTGAGG TTTCTCCAGC CTGTGGTCGA AACAGATTAC CTACACCATC TCCTGAGCCA
    CCTCCGAGTA TTGTGAGGTT TGCACTGCCG CCTGGGCACA CAAATAATGG GTCAGATTCG
    TCTGATTCAG AGTACAGCTC TCAAACCACC GTGTCAGGCA TCAGTGAGGA GCTCCATCAT
    TATGAGGCCA CCCAAAGCCC TGGGATACCA GTCCATCAAG TAGTAGTGGA AGCCACTGAG
    AATCCTGTCT TTGCAAGATC CACTGTGGTA CAGCCAGAGT CAAGGCATCA GTCAAGTCCC
    AGATTGCAAT CAAATCCAGA GGCTGGCACA CAGCAGGTGT GGCATCAGGG CAGACAACCC
    AAGCAGGAAG TGAGGGAAGG ACTGCGACCA CCTCCTTACC GCCCTCGCAG GGACGCTTTT
    GAAATTTCTA CTGAAGGGCA TTCAGGACCT AGCAATAAGG ATCGCTTGAA CCACAAGGCT
    CATTCTCATA ACATGAGAAG CCCAGCATTT GGTGCCATGG GTGTTCCAGG ATCTGCCTAC
    TGCCAGCCTA TCACTACTGT GACTGCTTCG GCTTCAGTGA CTGTTGCTGT CCACCCTGCT
    GTGCACAGCC ACAACTCTTG CAGAGGGAGC TTTCCATCCT GTGAAGAATA CAATGAAGAT
    GACCGTGAGA TGTTTGAGGA CCCCCATGTG CCTTTTAATG TGCGGTGTGA AAGAAGAAAT
    TCTAAAGTAG AAGTTATAGA GCTTCAGGAT GTCGAATGTG AGGAGAGGAC AGCTGGCAAA
    ATCTCAGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_990291.2
    CDS19..4350
    Translation

    Target ORF information:

    RefSeq Version NM_204960.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus patched 1 (PTCH1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_204960.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCCTCGG CCGCTGACGC CTTAGAGCCG GAGAGCGGCA GCAGCACCGC GGGCGGCGGC 
    AGCCACCCCG TTCGGGCGGC GAGGAGTGCC CGCGGCCGGC GGCGGCGGAG CGGAGGGACC
    CGGCGCGCCG CGGCGCCCGA CCGGGAGTAT CTGCAGCGGC CGAGCTACTG CGATGCGGCG
    TTCGCCTTGG AGCAGATCGC AAAGGGGAGA GCTACCGGGA GGAGAGCGCC GCTGTGGCTG
    CGGGCGAAGT TTCAGAGACT GCTCTTTAAC CTGGGCTGCT ACATTCAGAA AAACTGCGGC
    AAGTTCCTGG TGGTCGGCCT GCTGATATTC GGCGCGTTCG CGGTGGGACT ACGGGCGGCC
    AACCTAGAGA CCAACGTAGA GGAGCTGTGG GTGGAAGTTG GTGGACGAGT GAGTCGGGAG
    TTAAACTACA CACGGCAGAA GATTGGAGAA GAGGCCATGT TTAACCCCCA GCTCATGATT
    CAGACTCCAC AGGAAGATGG AACTAATGTA CTCACAACAG AAGCACTCCG GCAGCACCTT
    GACTCTGCAC TTCAGGCCAG CAGAGTACAT GTTTATATGT ACAACAGACA ATGGAAGTTG
    GAACATTTAT GTTATAAATC AGGAGAACTC ATCACAGAAG CAGGCTATAT GGACCAGATT
    ATAGAATATC TTTATCCTTG CTTAATTATC ACTCCTTTGG ACTGCTTCTG GGAGGGAGCA
    AAGCTACAGT CTGGAACTGC CTATCTATTA GGGAAGCCTC CTTTGCAGTG GATCAACTTT
    GACCCCTTAG AATTCTTGGA AGAGTTAAAG AAAATAAACT ACCAAGTGGA GAGCTGGGAA
    GAAATGCTGA ATAAAGCAGA GGTTGGTCAT GGTTATATGG ATCGACCTTG CCTGAATCCT
    GCTGATCCTG ATTGCCCAAT CACAGCTCCC AATAAAAATT CTACCAAGCC TCTTGATGTA
    GCCCTTGTTT TGAGTGGTGG ATGCTATGGA CTGTCAAGAA AATACATGCA TTGGCAGGAA
    GAGTTAATTA TAGGTGGTAC AGTCAAGAAC AGTTCTGGTA AACTTGTCAG TGCCCAGGCT
    TTGCAAACCA TGTTTCAGTT AATGACTCCT AAGCAAATGT ATGAGCACTT CAAGGGATAT
    GAATATGTTT CACATATCAA CTGGAATGAG GATAAAGCAG CAGCAATTCT GGAAGCCTGG
    CAGAGGATGT ATGTCGAGGT TGTTCATCAA AGTGTTGCAC AAAACTCTAC TCAGAAGGTG
    CTTTCCTTTA CTACAACTAC CCTGGACGAC ATCCTAAAGT CATTTTCTGA TGTCAGTGTT
    ATCAGAGTAG CTAGTGGCTA CTTACTAATG CTTGCCTACG CCTGTTTAAC AATGCTGAGG
    TGGGATTGTG CCAAGTCTCA AGGTGCCGTG GGGCTGGCAG GAGTCTTGTT GGTTGCACTG
    TCAGTGGCTG CAGGCTTGGG TCTGTGCTCA CTGATTGGAA TTTCCTTTAA TGCTGCCACA
    ACTCAGGTTC TGCCTTTTCT TGCTCTCGGA GTTGGTGTAG ATGATGTTTT CCTTCTAGCC
    CATGCGTTTA GTGAAACAGG ACAGAATAAG AGAATTCCAT TCGAGGACAG AACAGGTGAA
    TGTTTAAAGC GAACAGGAGC AAGTGTAGCC CTTACATCTA TCAGCAATGT TACTGCATTC
    TTTATGGCTG CCTTAATCCC TATTCCTGCT TTACGAGCAT TTTCACTCCA AGCAGCAGTT
    GTGGTAGTAT TCAACTTTGC AATGGTTCTG CTAATTTTTC CTGCTATACT GAGCATGGAC
    CTTTATCGTC GAGAAGACAG GAGACTGGAT ATATTCTGCT GTTTTACAAG TCCATGTGTC
    ACCAGAGTGA TTCAGATCGA GCCTCAAGCG TATGCAGAAA ATGACAATAT TTGTTACAGT
    TCTCCACCCC CGTACAGCAG TCACAGTTTT GCACATGAAA CTCAGATTAC AATGCAGTCT
    ACAGTGCAGT TGCGCACTGA ATATGATCCT CATACACAAG CGTACTACAC CACTGCAGAG
    CCACGATCAG AAATATCAGT ACAGCCTGTG ACAGTGACTC AAGATAGTCT TAGCTGCCAA
    AGCCCAGAAA GCGCAAGCTC AACAAGGGAT TTGCTTTCCC AGTTCTCAGA CTCCAGTGTG
    CATTGCCTTG AGCCACCCTG TACGAAGTGG ACACTCTCAA CTTTTGCAGA AAAACATTAT
    GCTCCGTTCC TCCTAAAACC AAAAGCTAAG GTTGTGGTTA TTTTTCTTTT CCTGGGCTTA
    CTTGGGCTCA GCCTCTATGG AACTACCCGG GTGAGAGATG GACTAGATCT CACAGACATT
    GTACCACGGG ATACACGGGA ATATGACTTT ATTGCTGCGC AGTTCAAATA CTTCTCATTC
    TACAATATGT ACATTGTGAC ACAGAAAGCA GACTATCCAA ATGTCCAGCA CTTACTTTAC
    GAACTTCACA GAAGTTTCAG CAATGTGACA TATGTTTTGT TGGAAGGAGA TAGGCAGCTT
    CCCAAAATGT GGTTACATTA CTTTCGAGAC TGGCTGCAAG GACTCCAGGA TGCATTTGAC
    AGTGACTGGG AAACTGGGAA AATCACTTAC AGCAACTATA AAAATGGATC TGATGATGCT
    GTTCTGGCTT ATAAACTCTT GGTACAAACA GGCAACCGAG CAAAGCCCAT TGACATCAGT
    CAGTTGACTA AACAGCGTCT GGTGGATGCA GATGGAATCA TTAACCCAAA TGCTTTCTAC
    ATCTACCTGA CAGCCTGGGT TAGCAATGAC CCTGTGGCCT ATGCTGCCTC TCAAGCCAAC
    ATCCGGCCTC ACCGCCCAGA ATGGGTGCAT GACAAAGCAG ACTATATGCC AGAAACAAGG
    CTAAGAATTC CAGCGGCTGA GCCCATAGAA TATGCTCAGT TTCCTTTCTA CCTCAATGGA
    TTACGTGAGA CTTCTGACTT TGTGGAAGCT ATTGAGAAAG TGAGAGCTAT CTGCAATAAC
    TACACAAGCC TCGGGATCGC TAGCTACCCA AATGGTTACC CATTTCTATT CTGGGAGCAG
    TACATTGGGC TTCGTCATTG GCTCCTCTTA TCCATTAGCG TGGTTTTGGC TTGCACGTTT
    CTGGTGTGTG CCCTCTTCCT CCTAAACCCC TGGACAGCTG GGATCATTGT GGTGGTGCTG
    GCTCTGATGA CTGTGGAGCT GTTTGGCATG ATGGGTTTAA TTGGAATAAA ACTAAGTGCA
    GTGCCTGTGG TTATCCTGAT TGCTTCTGTT GGAATTGGTG TGGAATTCAC TGTTCACATT
    GCTTTGGCAT TTTTAACTGC CATAGGAGAT AAGAACCGTA GGGCTGTCCT TGCATTGGAA
    CATATGTTTG CACCAGTGTT GGATGGAGCT GTGTCCACTC TTCTTGGAGT GTTAATGCTT
    GCAGGATCAG AGTTTGATTT TATTGTCAGG TATTTCTTTG CTGTTTTGGC AATCTTAACC
    ATTCTGGGAG TTCTCAATGG ATTGGTGCTG CTTCCTGTTC TTCTTTCTTT CTTTGGACCA
    TACCCTGAGG TTTCTCCAGC CTGTGGTCGA AACAGATTAC CTACACCATC TCCTGAGCCA
    CCTCCGAGTA TTGTGAGGTT TGCACTGCCG CCTGGGCACA CAAATAATGG GTCAGATTCG
    TCTGATTCAG AGTACAGCTC TCAAACCACC GTGTCAGGCA TCAGTGAGGA GCTCCATCAT
    TATGAGGCCA CCCAAAGCCC TGGGATACCA GTCCATCAAG TAGTAGTGGA AGCCACTGAG
    AATCCTGTCT TTGCAAGATC CACTGTGGTA CAGCCAGAGT CAAGGCATCA GTCAAGTCCC
    AGATTGCAAT CAAATCCAGA GGCTGGCACA CAGCAGGTGT GGCATCAGGG CAGACAACCC
    AAGCAGGAAG TGAGGGAAGG ACTGCGACCA CCTCCTTACC GCCCTCGCAG GGACGCTTTT
    GAAATTTCTA CTGAAGGGCA TTCAGGACCT AGCAATAAGG ATCGCTTGAA CCACAAGGCT
    CATTCTCATA ACATGAGAAG CCCAGCATTT GGTGCCATGG GTGTTCCAGG ATCTGCCTAC
    TGCCAGCCTA TCACTACTGT GACTGCTTCG GCTTCAGTGA CTGTTGCTGT CCACCCTGCT
    GTGCACAGCC ACAACTCTTG CAGAGGGAGC TTTCCATCCT GTGAAGAATA CAATGAAGAT
    GACCGTGAGA TGTTTGAGGA CCCCCATGTG CCTTTTAATG TGCGGTGTGA AAGAAGAAAT
    TCTAAAGTAG AAGTTATAGA GCTTCAGGAT GTCGAATGTG AGGAGAGGAC AGCTGGCAAA
    ATCTCAGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.