SPON1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPON1
Entrez Gene ID	395657
Full Name	spondin 1
Synonyms	spondin-1
General protein information	Preferred Names spondin 1 Names spondin-1 F-spondin spondin 1, extracellular matrix protein
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_204851.1	NP_990182.1	spondin-1 precursor
XM_025150570.1	XP_025006338.1	spondin-1 isoform X1

Pathways from BioSystems

Homologies

	SPON1	XP_001093336.1
Mus musculus (house mouse)	Spon1	NP_663559.1
Xenopus tropicalis (tropical clawed frog)	spon1	NP_001090741.1
Homo sapiens (human)	SPON1	NP_006099.2
Rattus norvegicus (Norway rat)	Spon1	NP_742064.1
Gallus gallus (chicken)	SPON1	NP_990182.1
Danio rerio (zebrafish)	spon1a	NP_571590.1
Drosophila melanogaster (fruit fly)	CG17739	NP_610757.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012307	XP_320234.3
Pan troglodytes (chimpanzee)	SPON1	XP_508295.3
Canis lupus familiaris (dog)	SPON1	XP_534067.2
Bos taurus (cattle)	SPON1	NP_777168.1

Gene Ontology
Bibliography

Related articles in PubMed

F-spondin regulates neuronal survival through activation of disabled-1 in the chicken ciliary ganglion.
Peterziel H, Sackmann T, Strelau J, Kuhn PH, Lichtenthaler SF, Marom K, Klar A, Unsicker K
Molecular and cellular neurosciences46(2)483-97(2011 Feb)

F-spondin regulates chondrocyte terminal differentiation and endochondral bone formation.
Palmer GD, Piton AH, Thant LM, Oliveira SM, D'Angelo M, Attur MG, Abramson SB, Teixeira CC
Journal of orthopaedic research : official publication of the Orthopaedic Research Society28(10)1323-9(2010 Oct)

F-Spondin is required for accurate pathfinding of commissural axons at the floor plate.
Burstyn-Cohen T, Tzarfaty V, Frumkin A, Feinstein Y, Stoeckli E, Klar A
Neuron23(2)233-46(1999 Jun)

F-Spondin, expressed in somite regions avoided by neural crest cells, mediates inhibition of distinct somite domains to neural crest migration.
Debby-Brafman A, Burstyn-Cohen T, Klar A, Kalcheim C
Neuron22(3)475-88(1999 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We report here that F-spondin is expressed in non-neuronal cells in the embryonic chicken ciliary ganglion (CG) and robustly promotes survival of cultured CG neurons
Title: F-spondin regulates neuronal survival through activation of disabled-1 in the chicken ciliary ganglion.

F-spondin is expressed in embryonic cartilage, where it has the capacity to enhance chondrocyte terminal differentiation and mineralization.
Title: F-spondin regulates chondrocyte terminal differentiation and endochondral bone formation.

The following SPON1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPON1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa27485	NM_204851.1 Latest version!	Gallus gallus spondin 1 (SPON1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OGa150747	XM_025150570.1 Latest version!	Gallus gallus spondin 1 (SPON1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPON1 ( NM_204851.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990182.1 Gallus gallus spondin 1 (SPON1), mRNA.

Price & Availability↑

CloneID	OGa27485
Clone ID Related Accession (Same CDS sequence)	NM_204851.1
Accession Version	NM_204851.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Gallus gallus(chicken)
Product	spondin-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF149302.1. ##Evidence-Data-START## Transcript exon combination :: AF149302.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCAGCGC GGCTGCGGCC CCTGGCCCTG CGGCTGCTGG CGCGCACCTT CCCCTTGGTG 
GCGAGGGGCT TCTCCGACGA GACCCTGGAG AAAGCCGCCA AATCCGAGGG CTACTGCAGC 
CGGATCCTGC GAGCCCAAGG CACCAGGAGG GAAGGGTACA ATGAATTTAG CCTGAGGGTG 
GAGGGCGATC CGGAATTCTA CAAGCCTGGG AACAGTTACC GCGTGACGCT TTCTGCTGCC 
ACTCCTGCGT ACTTTCGAGG ATTCACATTG ATTGCTCTGA AGGAAGGAAA AGAAGGTGAT 
AAAGAGGAAG ACCATGCGGG AACTTTTCAG ATCATAGATG AAGAAGAGAC GCAGTTCATG 
AGCAATTGTC CCGTCGCGGT TACTGAGAGC ACACCTAGAA GGAGGACACG CATCCAGGTC 
TTCTGGACAG CTCCTCCTAC TGGTACGGGC TGTGTCATTC TGAAAGCCAG TATTGTGCAG 
AAGCGCATTA TTTATTTTCA GGACGAGGGT TCTCTCACCA AAAGAATCTG TGAACAAGAT 
TCAGCCTCTG AAGGTGTGAC TGACAAACCA ACATTAGATT GCTGTGCCTG TGGAACTGCC 
AAATACAGGC TAACGTTTTA TGGAAATTGG TCGGAAAAAA CACATCCCAA AGACTTTCCT 
CGGCGCACCA ACCATTGGTC TGCGATCATT GGTAGCTCTC ACTCAAAGAA CTACATCCTT 
TGGGAGTATG GAGGGTATGC TAGTGAAGGT GTCAAGCAGG TTGCAGAGCT GGGATCCCCA 
GTCAAGATGG AAGAAGAAAT TCGACAACAA AGTGATGAGG TTTTAACAGT CATCAAGGCA 
AAAGCACAGT GGCCTGCCTG GCAGCCTCTG AATGTGAGAG CTGCTCCCTC TGCTGAGTTT 
TCTGTTGATC GCCACCGGCA CCTGATGTCC TTCCTCACCA TGCTGGGGCC CAGTCCCGAC 
TGGAATGTGG GCCTGTCTGC TGAGGACCTC TGCACCAAGG ACTGTGGCTG GGTTCAGAAA 
GTCGTGCAGG ATTTAATCCC CTGGGATGCC GGCACAGACA GTGGCGTCAC CTATGAGTCA 
CCCAACAAAC CTACAGTTCC TCAAGAGAAG ATTAGACCAC TTACAAGCTT AGATCACCCT 
CAGAGTCCAT TTTATGATCC AGAAGGAGGA TCTATCAAGC TTGTAGCCAG AGTCGTGCTT 
GAAAGAATTG CACGCAAGGG GGAGCAGTGC AACTTCGTAC CTGATAACAT AGATGATATT 
GTGGCAGACC TAGCACCAGA AGAAAAAGAA GAAGATGATA CCCCTGAGAC CTGCATATAT 
TCAAACTGGT CCCCCTGGTC AGCCTGCAGC TCCTCTACCT GTGAGAAGGG CAAGAGGATG 
AGGCAGAGAA TGCTTAAAGC TCAGCTGGAC CTCAGTGTGC CCTGTCCTGA TACCCAAGAT 
TTTCAGCCAT GCATGGGTCC AGGCTGCAGT GATGAAGATG GTTCAACTTG CATGATGTCT 
GACTGGATTA CATGGTCCCC CTGTAGTGTT TCCTGTGGAA TGGGAACGCG ATCTAGAGAG 
AGATATGTAA AGCAATTCCC CGAAGATGGC TCTATGTGCA AAGTGCCTAC TGAAGAAACT 
GAGAAATGTA TTGTAAATGA GGAATGCTCC CCTAGCAGCT GCCTTGTCAC CGAATGGGGA 
GAGTGGGATG AATGCAGTGC TAGCTGTGGC ACAGGAATGA AAAGGCGACA CAGAATGATC 
AAGATGACTC CTGCTGATGG ATCTATGTGC AAGGCAGAAA CTACAGAGGC AGAGAAATGC 
ATGATGCCCG AATGCCATAC TATTCCCTGC CTTCTATCCC CATGGTCTGA ATGGAGCGAC 
TGCAGCGTGA CATGTGGGAA GGGAATGCGA ACCCGGCAAA GGATGCTGAA ATCTGCAGCT 
GAGCTTGGAG ACTGCAATGA GGAACTGGAG CAAGCAGAGA AATGCATGCT ACCTGAATGC 
CCCATTGACT GTGAGCTAAC GGAGTGGTCC CAGTGGTCCG AGTGCAATAC CTCCTGTGGG 
AAGGGCCACA TGATCAGAAC AAGAATGATC AAAATAGAAC CACAGTTTGG AGGAACAGCA 
TGCCCAGAAA CTGTCCAACG TACTAAATGT CGAGTAAGGA AATGCCTGAG AGGCCCAGGT 
ATGGAAAAGA GGCGTTGGAA GGAGGCCCGG GAGAAAAGAA GAAGTGAACA AGCAAAAAAA 
AATATTGATA ATGAGCAATA TCCAGTTTGT AGGCTGAAAC CATGGACTGC TTGGACAGAA 
TGTTCTACAC TCTGTGGAGG TGGAATTCAG GAGCGCTACA TGATGGTAAA GAAGAGGTCC 
AAAAGCACTC AGTTTACTAG CTGCAAAGAC AAAAAGGAGC TAAGAGCATG TAACGTTCAT 
CCTTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990182.1
CDS	136..2544
Translation	MAARLRPLALRLLARTFPLVARGFSDETLEKAAKSEGYCSRILRAQGTRREGYNEFSLRVEGDPEFYKPGNSYRVTLSAATPAYFRGFTLIALKEGKEGDKEEDHAGTFQIIDEEETQFMSNCPVAVTESTPRRRTRIQVFWTAPPTGTGCVILKASIVQKRIIYFQDEGSLTKRICEQDSASEGVTDKPTLDCCACGTAKYRLTFYGNWSEKTHPKDFPRRTNHWSAIIGSSHSKNYILWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPLNVRAAPSAEFSVDRHRHLMSFLTMLGPSPDWNVGLSAEDLCTKDCGWVQKVVQDLIPWDAGTDSGVTYESPNKPTVPQEKIRPLTSLDHPQSPFYDPEGGSIKLVARVVLERIARKGEQCNFVPDNIDDIVADLAPEEKEEDDTPETCIYSNWSPWSACSSSTCEKGKRMRQRMLKAQLDLSVPCPDTQDFQPCMGPGCSDEDGSTCMMSDWITWSPCSVSCGMGTRSRERYVKQFPEDGSMCKVPTEETEKCIVNEECSPSSCLVTEWGEWDECSASCGTGMKRRHRMIKMTPADGSMCKAETTEAEKCMMPECHTIPCLLSPWSEWSDCSVTCGKGMRTRQRMLKSAAELGDCNEELEQAEKCMLPECPIDCELTEWSQWSECNTSCGKGHMIRTRMIKIEPQFGGTACPETVQRTKCRVRKCLRGPGMEKRRWKEAREKRRSEQAKKNIDNEQYPVCRLKPWTAWTECSTLCGGGIQERYMMVKKRSKSTQFTSCKDKKELRACNVHPC

Target ORF information:

RefSeq Version	NM_204851.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spondin 1 (SPON1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204851.1

ORF Insert Sequence:

ATGGCAGCGC GGCTGCGGCC CCTGGCCCTG CGGCTGCTGG CGCGCACCTT CCCCTTGGTG 
GCGAGGGGCT TCTCCGACGA GACCCTGGAG AAAGCCGCCA AATCCGAGGG CTACTGCAGC 
CGGATCCTGC GAGCCCAAGG CACCAGGAGG GAAGGGTACA ATGAATTTAG CCTGAGGGTG 
GAGGGCGATC CGGAATTCTA CAAGCCTGGG AACAGTTACC GCGTGACGCT TTCTGCTGCC 
ACTCCTGCGT ACTTTCGAGG ATTCACATTG ATTGCTCTGA AGGAAGGAAA AGAAGGTGAT 
AAAGAGGAAG ACCATGCGGG AACTTTTCAG ATCATAGATG AAGAAGAGAC GCAGTTCATG 
AGCAATTGTC CCGTCGCGGT TACTGAGAGC ACACCTAGAA GGAGGACACG CATCCAGGTC 
TTCTGGACAG CTCCTCCTAC TGGTACGGGC TGTGTCATTC TGAAAGCCAG TATTGTGCAG 
AAGCGCATTA TTTATTTTCA GGACGAGGGT TCTCTCACCA AAAGAATCTG TGAACAAGAT 
TCAGCCTCTG AAGGTGTGAC TGACAAACCA ACATTAGATT GCTGTGCCTG TGGAACTGCC 
AAATACAGGC TAACGTTTTA TGGAAATTGG TCGGAAAAAA CACATCCCAA AGACTTTCCT 
CGGCGCACCA ACCATTGGTC TGCGATCATT GGTAGCTCTC ACTCAAAGAA CTACATCCTT 
TGGGAGTATG GAGGGTATGC TAGTGAAGGT GTCAAGCAGG TTGCAGAGCT GGGATCCCCA 
GTCAAGATGG AAGAAGAAAT TCGACAACAA AGTGATGAGG TTTTAACAGT CATCAAGGCA 
AAAGCACAGT GGCCTGCCTG GCAGCCTCTG AATGTGAGAG CTGCTCCCTC TGCTGAGTTT 
TCTGTTGATC GCCACCGGCA CCTGATGTCC TTCCTCACCA TGCTGGGGCC CAGTCCCGAC 
TGGAATGTGG GCCTGTCTGC TGAGGACCTC TGCACCAAGG ACTGTGGCTG GGTTCAGAAA 
GTCGTGCAGG ATTTAATCCC CTGGGATGCC GGCACAGACA GTGGCGTCAC CTATGAGTCA 
CCCAACAAAC CTACAGTTCC TCAAGAGAAG ATTAGACCAC TTACAAGCTT AGATCACCCT 
CAGAGTCCAT TTTATGATCC AGAAGGAGGA TCTATCAAGC TTGTAGCCAG AGTCGTGCTT 
GAAAGAATTG CACGCAAGGG GGAGCAGTGC AACTTCGTAC CTGATAACAT AGATGATATT 
GTGGCAGACC TAGCACCAGA AGAAAAAGAA GAAGATGATA CCCCTGAGAC CTGCATATAT 
TCAAACTGGT CCCCCTGGTC AGCCTGCAGC TCCTCTACCT GTGAGAAGGG CAAGAGGATG 
AGGCAGAGAA TGCTTAAAGC TCAGCTGGAC CTCAGTGTGC CCTGTCCTGA TACCCAAGAT 
TTTCAGCCAT GCATGGGTCC AGGCTGCAGT GATGAAGATG GTTCAACTTG CATGATGTCT 
GACTGGATTA CATGGTCCCC CTGTAGTGTT TCCTGTGGAA TGGGAACGCG ATCTAGAGAG 
AGATATGTAA AGCAATTCCC CGAAGATGGC TCTATGTGCA AAGTGCCTAC TGAAGAAACT 
GAGAAATGTA TTGTAAATGA GGAATGCTCC CCTAGCAGCT GCCTTGTCAC CGAATGGGGA 
GAGTGGGATG AATGCAGTGC TAGCTGTGGC ACAGGAATGA AAAGGCGACA CAGAATGATC 
AAGATGACTC CTGCTGATGG ATCTATGTGC AAGGCAGAAA CTACAGAGGC AGAGAAATGC 
ATGATGCCCG AATGCCATAC TATTCCCTGC CTTCTATCCC CATGGTCTGA ATGGAGCGAC 
TGCAGCGTGA CATGTGGGAA GGGAATGCGA ACCCGGCAAA GGATGCTGAA ATCTGCAGCT 
GAGCTTGGAG ACTGCAATGA GGAACTGGAG CAAGCAGAGA AATGCATGCT ACCTGAATGC 
CCCATTGACT GTGAGCTAAC GGAGTGGTCC CAGTGGTCCG AGTGCAATAC CTCCTGTGGG 
AAGGGCCACA TGATCAGAAC AAGAATGATC AAAATAGAAC CACAGTTTGG AGGAACAGCA 
TGCCCAGAAA CTGTCCAACG TACTAAATGT CGAGTAAGGA AATGCCTGAG AGGCCCAGGT 
ATGGAAAAGA GGCGTTGGAA GGAGGCCCGG GAGAAAAGAA GAAGTGAACA AGCAAAAAAA 
AATATTGATA ATGAGCAATA TCCAGTTTGT AGGCTGAAAC CATGGACTGC TTGGACAGAA 
TGTTCTACAC TCTGTGGAGG TGGAATTCAG GAGCGCTACA TGATGGTAAA GAAGAGGTCC 
AAAAGCACTC AGTTTACTAG CTGCAAAGAC AAAAAGGAGC TAAGAGCATG TAACGTTCAT 
CCTTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPON1 ( XM_025150570.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025006338.1 Gallus gallus spondin 1 (SPON1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa150747
Clone ID Related Accession (Same CDS sequence)	XM_025150570.1
Accession Version	XM_025150570.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	spondin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCACT CAGTGACGCT TTCTGCTGCC ACTCCTGCGT ACTTTCGAGG ATTCACATTG 
ATTGCTCTGA AGGAAGGAAA AGAAGGTGAT AAAGAGGAAG ACCATGCGGG AACTTTTCAG 
ATCATAGATG AAGAAGAGAC GCAGTTCATG AGCAATTGTC CCGTCGCGGT TACTGAGAGC 
ACACCTAGAA GGAGGACACG CATCCAGGTC TTCTGGACAG CTCCTCCTAC TGGTACGGGC 
TGTGTCATTC TGAAAGCCAG TATTGTGCAG AAGCGCATTA TTTATTTTCA GGACGAGGGT 
TCTCTCACCA AAAGAATCTG TGAACAAGAT TCAGCCTCTG AAGGTGTGAC TGACAAACCA 
ATATTAGATT GCTGTGCCTG TGGAACTGCC AAATACAGGC TAACGTTTTA TGGAAATTGG 
TCGGAAAAAA CACATCCCAA AGACTTTCCT CGGCGCACCA ACCATTGGTC TGCGATCATT 
GGTAGCTCTC ACTCAAAGAA CTACATCCTT TGGGAGTATG GAGGGTATGC TAGTGAAGGT 
GTCAAGCAGG TTGCAGAGCT GGGATCCCCA GTCAAGATGG AAGAAGAAAT TCGACAACAA 
AGTGATGAGG TTTTAACAGT CATCAAGGCA AAAGCACAGT GGCCTGCCTG GCAGCCTCTG 
AATGTGAGAG CTGCTCCCTC TGCTGAGTTT TCTGTTGATC GCCACCGGCA CCTGATGTCC 
TTCCTCACCA TGCTGGGGCC CAGTCCCGAC TGGAATGTGG GCCTGTCTGC TGAGGACCTC 
TGCACCAAGG ACTGTGGCTG GGTTCAGAAA GTCGTGCAGG ATTTAATCCC CTGGGATGCC 
GGCACAGACA GTGGCGTCAC CTATGAGTCA CCCAACAAAC CTACAGTTCC TCAAGAGAAG 
ATTAGACCAC TTACAAGCTT AGATCACCCT CAGAGTCCAT TTTATGATCC AGAAGGAGGA 
TCTATCAAGC TTGTAGCCAG AGTCGTCATT GAAAGAATTG CACGCAAGGG GGAGCAGTGC 
AACATCGTAC CTGATAACAT AGATGATATT GTGGCAGACC TAGCACCAGA AGAAAAAGAA 
GAAGATGATA CCCCTGAGAC CTGCATATAT TCAAACTGGT CCCCCTGGTC AGCCTGCAGC 
TCCTCTACCT GTGAGAAGGG CAAGAGGATG AGGCAGAGAA TGCTTAAAGC TCAGCTGGAC 
CTCAGTGTGC CCTGTCCTGA TACCCAAGAT TTTCAGCCAT GCATGGGTCC AGGCTGCAGT 
GATGAAGATG GTTCAACTTG CATGATGTCT GACTGGATTA CATGGTCCCC CTGTAGTGTT 
TCCTGTGGAA TGGGAACGCG ATCTAGAGAG AGATATGTAA AGCAATTCCC CGAAGATGGC 
TCTATGTGCA AAGTGCCTAC TGAAGAAACT GAGAAATGTA TTGTAAATGA GGAATGCTCC 
CCTAGCAGCT GCCTTGTCAC CGAATGGGGA GAGTGGGATG AATGCAGTGC TAGCTGTGGC 
ACGGGAATGA AAAGGCGACA CAGAATGATC AAGATGACTC CTGCTGATGG ATCTATGTGC 
AAGGCAGAAA CTACAGAGGC AGAGAAATGC ATGATGCCCG AATGCCATAC TATTCCCTGC 
CTTCTATCCC CATGGTCTGA ATGGAGCGAC TGCAGCGTGA CTTGTGGGAA GGGAATGCGA 
ACCCGGCAAA GGATGCTGAA ATCTGCAGCT GAGCTTGGAG ACTGCAATGA GGAACTGGAG 
CAAGCAGAGA AATGCATGCT ACCTGAATGC CCCATTGACT GTGAGCTAAC GGAGTGGTCC 
CAGTGGTCCG AGTGCAATAC CTCCTGTGGG AAGGGCCACA TGATCAGAAC AAGAATGATC 
AAAATAGAAC CACAGTTTGG AGGAACAGCA TGCCCAGAAA CTGTCCAACG TACTAAATGT 
CGAGTAAGGA AATGCCTGAG AGGCCCAGGT ATGGAAAAGA GGCGTTGGAA GGAGGCCCGG 
GAGAAAAGAA GAAGTGAACA AGCAAAAAAA AATATTGATA ATGAGCAATA TCCAGTTTGT 
AGGCTGAAAC CATGGACTGC TTGGACAGAA TGTTCTACAC TCTGTGGAGG TGGAATTCAG 
GAGCGCTACA TGATGGTAAA GAAGAGGTCC AAAAGCACTC AGTTTACTAG CTGCAAAGAC 
AAAAAGGAGC TAAGAGCATG TAACGTTCAT CCTTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025006338.1
CDS	16..2214
Translation	MLHSVTLSAATPAYFRGFTLIALKEGKEGDKEEDHAGTFQIIDEEETQFMSNCPVAVTESTPRRRTRIQVFWTAPPTGTGCVILKASIVQKRIIYFQDEGSLTKRICEQDSASEGVTDKPILDCCACGTAKYRLTFYGNWSEKTHPKDFPRRTNHWSAIIGSSHSKNYILWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPLNVRAAPSAEFSVDRHRHLMSFLTMLGPSPDWNVGLSAEDLCTKDCGWVQKVVQDLIPWDAGTDSGVTYESPNKPTVPQEKIRPLTSLDHPQSPFYDPEGGSIKLVARVVIERIARKGEQCNIVPDNIDDIVADLAPEEKEEDDTPETCIYSNWSPWSACSSSTCEKGKRMRQRMLKAQLDLSVPCPDTQDFQPCMGPGCSDEDGSTCMMSDWITWSPCSVSCGMGTRSRERYVKQFPEDGSMCKVPTEETEKCIVNEECSPSSCLVTEWGEWDECSASCGTGMKRRHRMIKMTPADGSMCKAETTEAEKCMMPECHTIPCLLSPWSEWSDCSVTCGKGMRTRQRMLKSAAELGDCNEELEQAEKCMLPECPIDCELTEWSQWSECNTSCGKGHMIRTRMIKIEPQFGGTACPETVQRTKCRVRKCLRGPGMEKRRWKEAREKRRSEQAKKNIDNEQYPVCRLKPWTAWTECSTLCGGGIQERYMMVKKRSKSTQFTSCKDKKELRACNVHPC

Target ORF information:

RefSeq Version	XM_025150570.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spondin 1 (SPON1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025150570.1

ORF Insert Sequence:

ATGCTTCACT CAGTGACGCT TTCTGCTGCC ACTCCTGCGT ACTTTCGAGG ATTCACATTG 
ATTGCTCTGA AGGAAGGAAA AGAAGGTGAT AAAGAGGAAG ACCATGCGGG AACTTTTCAG 
ATCATAGATG AAGAAGAGAC GCAGTTCATG AGCAATTGTC CCGTCGCGGT TACTGAGAGC 
ACACCTAGAA GGAGGACACG CATCCAGGTC TTCTGGACAG CTCCTCCTAC TGGTACGGGC 
TGTGTCATTC TGAAAGCCAG TATTGTGCAG AAGCGCATTA TTTATTTTCA GGACGAGGGT 
TCTCTCACCA AAAGAATCTG TGAACAAGAT TCAGCCTCTG AAGGTGTGAC TGACAAACCA 
ATATTAGATT GCTGTGCCTG TGGAACTGCC AAATACAGGC TAACGTTTTA TGGAAATTGG 
TCGGAAAAAA CACATCCCAA AGACTTTCCT CGGCGCACCA ACCATTGGTC TGCGATCATT 
GGTAGCTCTC ACTCAAAGAA CTACATCCTT TGGGAGTATG GAGGGTATGC TAGTGAAGGT 
GTCAAGCAGG TTGCAGAGCT GGGATCCCCA GTCAAGATGG AAGAAGAAAT TCGACAACAA 
AGTGATGAGG TTTTAACAGT CATCAAGGCA AAAGCACAGT GGCCTGCCTG GCAGCCTCTG 
AATGTGAGAG CTGCTCCCTC TGCTGAGTTT TCTGTTGATC GCCACCGGCA CCTGATGTCC 
TTCCTCACCA TGCTGGGGCC CAGTCCCGAC TGGAATGTGG GCCTGTCTGC TGAGGACCTC 
TGCACCAAGG ACTGTGGCTG GGTTCAGAAA GTCGTGCAGG ATTTAATCCC CTGGGATGCC 
GGCACAGACA GTGGCGTCAC CTATGAGTCA CCCAACAAAC CTACAGTTCC TCAAGAGAAG 
ATTAGACCAC TTACAAGCTT AGATCACCCT CAGAGTCCAT TTTATGATCC AGAAGGAGGA 
TCTATCAAGC TTGTAGCCAG AGTCGTCATT GAAAGAATTG CACGCAAGGG GGAGCAGTGC 
AACATCGTAC CTGATAACAT AGATGATATT GTGGCAGACC TAGCACCAGA AGAAAAAGAA 
GAAGATGATA CCCCTGAGAC CTGCATATAT TCAAACTGGT CCCCCTGGTC AGCCTGCAGC 
TCCTCTACCT GTGAGAAGGG CAAGAGGATG AGGCAGAGAA TGCTTAAAGC TCAGCTGGAC 
CTCAGTGTGC CCTGTCCTGA TACCCAAGAT TTTCAGCCAT GCATGGGTCC AGGCTGCAGT 
GATGAAGATG GTTCAACTTG CATGATGTCT GACTGGATTA CATGGTCCCC CTGTAGTGTT 
TCCTGTGGAA TGGGAACGCG ATCTAGAGAG AGATATGTAA AGCAATTCCC CGAAGATGGC 
TCTATGTGCA AAGTGCCTAC TGAAGAAACT GAGAAATGTA TTGTAAATGA GGAATGCTCC 
CCTAGCAGCT GCCTTGTCAC CGAATGGGGA GAGTGGGATG AATGCAGTGC TAGCTGTGGC 
ACGGGAATGA AAAGGCGACA CAGAATGATC AAGATGACTC CTGCTGATGG ATCTATGTGC 
AAGGCAGAAA CTACAGAGGC AGAGAAATGC ATGATGCCCG AATGCCATAC TATTCCCTGC 
CTTCTATCCC CATGGTCTGA ATGGAGCGAC TGCAGCGTGA CTTGTGGGAA GGGAATGCGA 
ACCCGGCAAA GGATGCTGAA ATCTGCAGCT GAGCTTGGAG ACTGCAATGA GGAACTGGAG 
CAAGCAGAGA AATGCATGCT ACCTGAATGC CCCATTGACT GTGAGCTAAC GGAGTGGTCC 
CAGTGGTCCG AGTGCAATAC CTCCTGTGGG AAGGGCCACA TGATCAGAAC AAGAATGATC 
AAAATAGAAC CACAGTTTGG AGGAACAGCA TGCCCAGAAA CTGTCCAACG TACTAAATGT 
CGAGTAAGGA AATGCCTGAG AGGCCCAGGT ATGGAAAAGA GGCGTTGGAA GGAGGCCCGG 
GAGAAAAGAA GAAGTGAACA AGCAAAAAAA AATATTGATA ATGAGCAATA TCCAGTTTGT 
AGGCTGAAAC CATGGACTGC TTGGACAGAA TGTTCTACAC TCTGTGGAGG TGGAATTCAG 
GAGCGCTACA TGATGGTAAA GAAGAGGTCC AAAAGCACTC AGTTTACTAG CTGCAAAGAC 
AAAAAGGAGC TAAGAGCATG TAACGTTCAT CCTTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

F-spondin regulates neuronal survival through activation of disabled-1 in the chicken ciliary ganglion.
Molecular and cellular neurosciences46(2)483-97(2011 Feb)
Peterziel H,Sackmann T,Strelau J,Kuhn PH,Lichtenthaler SF,Marom K,Klar A,Unsicker K

F-spondin regulates chondrocyte terminal differentiation and endochondral bone formation.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society28(10)1323-9(2010 Oct)
Palmer GD,Piton AH,Thant LM,Oliveira SM,D'Angelo M,Attur MG,Abramson SB,Teixeira CC

F-Spondin is required for accurate pathfinding of commissural axons at the floor plate.
Neuron23(2)233-46(1999 Jun)
Burstyn-Cohen T,Tzarfaty V,Frumkin A,Feinstein Y,Stoeckli E,Klar A

F-Spondin, expressed in somite regions avoided by neural crest cells, mediates inhibition of distinct somite domains to neural crest migration.
Neuron22(3)475-88(1999 Mar)
Debby-Brafman A,Burstyn-Cohen T,Klar A,Kalcheim C

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SPON1 cDNA ORF clone, Gallus gallus(chicken)

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Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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