gRNAs
The following NOTCH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOTCH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OGa00576 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001030295.1 | |
| Accession Version | NM_001030295.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 7686bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-04-01 | |
| Organism | Gallus gallus(chicken) | |
| Product | neurogenic locus notch homolog protein 1 | |
| Comment | Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AADN05000150.1. On May 28, 2014 this sequence version replaced XM_415420.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201357, SAMEA2201358 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGGGCGGT GTAGTGCTGC CCATCCCCGC GGTGGGGTGC ATTGCCCCGG GCTCTGTGCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001025466.1 |
| CDS | 1..7686 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001030295.1 |
1 | ATGGGGCGGT GTAGTGCTGC CCATCCCCGC GGTGGGGTGC ATTGCCCCGG GCTCTGTGCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa43346 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015279324.1 , XM_015279324.2 | |
| Accession Version | XM_015279324.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 7656bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-01-03 | |
| Organism | Gallus gallus(chicken) | |
| Product | neurogenic locus notch homolog protein 1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAAGCGGC TCTTGGCCAC CGGGCTCCTG CTGGCCCTGC TGCCCGCCCC GACGCGAGGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015134810.1 |
| CDS | 189..7844 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015279324.1 |
1 | ATGAAGCGGC TCTTGGCCAC CGGGCTCCTG CTGGCCCTGC TGCCCGCCCC GACGCGAGGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa43346 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015279324.1 , XM_015279324.2 | |
| Accession Version | XM_015279324.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 7656bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-16 | |
| Organism | Gallus gallus(chicken) | |
| Product | neurogenic locus notch homolog protein 1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279324.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAAGCGGC TCTTGGCCAC CGGGCTCCTG CTGGCCCTGC TGCCCGCCCC GACGCGAGGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015134810.1 |
| CDS | 191..7846 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015279324.2 |
1 | ATGAAGCGGC TCTTGGCCAC CGGGCTCCTG CTGGCCCTGC TGCCCGCCCC GACGCGAGGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa43347 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015279325.2 , XM_015279325.1 | |
| Accession Version | XM_015279325.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 7518bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-16 | |
| Organism | Gallus gallus(chicken) | |
| Product | neurogenic locus notch homolog protein 1 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279325.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAAGCGGC TCTTGGCCAC CGGGCTCCTG CTGGCCCTGC TGCCCGCCCC GACGCGAGGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015134811.1 |
| CDS | 191..7708 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015279325.2 |
1 | ATGAAGCGGC TCTTGGCCAC CGGGCTCCTG CTGGCCCTGC TGCCCGCCCC GACGCGAGGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa43347 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015279325.2 , XM_015279325.1 | |
| Accession Version | XM_015279325.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 7518bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-01-03 | |
| Organism | Gallus gallus(chicken) | |
| Product | neurogenic locus notch homolog protein 1 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAAGCGGC TCTTGGCCAC CGGGCTCCTG CTGGCCCTGC TGCCCGCCCC GACGCGAGGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015134811.1 |
| CDS | 189..7706 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015279325.1 |
1 | ATGAAGCGGC TCTTGGCCAC CGGGCTCCTG CTGGCCCTGC TGCCCGCCCC GACGCGAGGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Muscle contraction is required to maintain the pool of muscle progenitors via YAP and NOTCH during fetal myogenesis. |
Cytoplasmic NOTCH and membrane-derived ?-catenin link cell fate choice to epithelial-mesenchymal transition during myogenesis. |
A conserved role for Notch signaling in priming the cellular response to Shh through ciliary localisation of the key Shh transducer Smo. |
Cadherin-based adhesions in the apical endfoot are required for active Notch signaling to control neurogenesis in vertebrates. |
