SHH cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	SHH
Entrez Gene ID	395615
Full Name	sonic hedgehog
Synonyms	ShhNC
General protein information	Preferred Names sonic hedgehog Names sonic hedgehog protein shh unprocessed N-terminal signaling and C-terminal autoprocessing domains sonic hedgehog homolog
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_204821.1	NP_990152.1	sonic hedgehog protein precursor

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	shh	XP_002932544.2
Homo sapiens (human)	SHH	NP_000184.1
Mus musculus (house mouse)	Shh	NP_033196.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001412	XP_321721.5
Bos taurus (cattle)	SHH	XP_002687031.2
Rattus norvegicus (Norway rat)	Shh	NP_058917.1
Gallus gallus (chicken)	SHH	NP_990152.1
Danio rerio (zebrafish)	shha	NP_571138.1
Danio rerio (zebrafish)	shhb	NP_571274.2
	SHH	XP_001106515.2
Canis lupus familiaris (dog)	SHH	XP_850450.1
Pan troglodytes (chimpanzee)	SHH	XP_001147185.2
Drosophila melanogaster (fruit fly)	hh	NP_001034065.1

Gene Ontology
Bibliography

Related articles in PubMed

Upstream regulation for initiation of restricted Shh expression in the chick limb bud.
Matsubara H, Saito D, Abe G, Yokoyama H, Suzuki T, Tamura K
Developmental dynamics : an official publication of the American Association of Anatomists246(5)417-430(2017 May)

SHH ventralizes the otocyst by maintaining basal PKA activity and regulating GLI3 signaling.
Ohta S, Wang B, Mansour SL, Schoenwolf GC
Developmental biology420(1)100-109(2016 Dec)

Splenic gene expression profiling in White Leghorn layer inoculated with the Salmonella enterica serovar Enteritidis.
Wu G, Liu L, Qi Y, Sun Y, Yang N, Xu G, Zhou H, Li X
Animal genetics46(6)617-26(2015 Dec)

Sonic hedgehog overexpression regulates the neuroepithelial cells proliferation in the spinal cord of dorsal regions during chicken embryo development.
Yang C, Li X, Liu Y, Li H, Guo Z, Lin J
Neuro endocrinology letters36(4)380-6(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

propose a model in which positive and negative transcriptional regulatory networks accumulate their functions in the intersection area of their expression regions to provide a restricted spot for the ZPA (zone of polarizing activity), the source of morphogen, Shh.
Title: Upstream regulation for initiation of restricted Shh expression in the chick limb bud.

cAMP dependent protein kinase A (PKA) is a key intracellular factor mediating SHH signaling through regulation of GLI3 processing.
Title: SHH ventralizes the otocyst by maintaining basal PKA activity and regulating GLI3 signaling.

These data identify Notch signalling as a novel modulator of Shh signalling that acts mechanistically via regulation of ciliary localisation of key components of its transduction machinery.
Title: A conserved role for Notch signaling in priming the cellular response to Shh through ciliary localisation of the key Shh transducer Smo.

Sonic Hedgehog has a conserved role in tonotopic organization of the avian basilar papilla and mammalian cochlea
Title: Conserved role of Sonic Hedgehog in tonotopic organization of the avian basilar papilla and mammalian cochlea.

SHH determines the formation of spinal cord during the development of chicken embryo.
Title: Sonic hedgehog overexpression regulates the neuroepithelial cells proliferation in the spinal cord of dorsal regions during chicken embryo development.

The following SHH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SHH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa00412	NM_204821.1 Latest version!	Gallus gallus sonic hedgehog (SHH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SHH ( NM_204821.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990152.1 Gallus gallus sonic hedgehog (SHH), mRNA.

Price & Availability↑

CloneID	OGa00412
Clone ID Related Accession (Same CDS sequence)	NM_204821.1
Accession Version	NM_204821.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Gallus gallus(chicken)
Product	sonic hedgehog protein precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L28099.1. Sequence Note: This sequence has been modified as follows: removed 16/16 bp suspected to be vector contamination from the 5'/3' end. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L28099.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTCGAAA TGCTGCTGTT GACAAGAATT CTCTTGGTGG GCTTCATCTG CGCTCTTTTA 
GTCTCCTCTG GGCTGACTTG TGGACCAGGC AGGGGCATTG GAAAAAGGAG GCACCCCAAA 
AAGCTGACCC CGTTAGCCTA TAAGCAGTTT ATTCCCAATG TGGCAGAGAA GACCCTAGGG 
GCCAGTGGAA GATATGAAGG GAAGATCACA AGAAACTCCG AGAGATTTAA AGAACTAACC 
CCAAATTACA ACCCTGACAT TATTTTTAAG GATGAAGAGA ACACGGGAGC TGACAGACTG 
ATGACTCAGC GCTGCAAGGA CAAGCTGAAT GCCCTGGCGA TCTCGGTGAT GAACCAGTGG 
CCCGGGGTGA AGCTGCGGGT GACCGAGGGC TGGGACGAGG ATGGCCATCA CTCCGAGGAA 
TCGCTGCACT ACGAGGGTCG CGCCGTGGAC ATCACCACGT CGGATCGGGA CCGCAGCAAG 
TACGGAATGC TGGCCCGCCT CGCCGTCGAG GCCGGCTTCG ACTGGGTCTA CTACGAGTCC 
AAGGCGCACA TCCACTGCTC CGTCAAAGCA GAAAACTCAG TGGCAGCGAA ATCAGGAGGC 
TGCTTCCCTG GCTCAGCCAC AGTGCACCTG GAGCATGGAG GCACCAAGCT GGTGAAGGAC 
CTGAGCCCTG GGGACCGCGT GCTGGCTGCT GACGCGGACG GCCGGCTGCT CTACAGTGAC 
TTCCTCACCT TCCTCGACCG GATGGACAGC TCCCGAAAGC TCTTCTACGT CATCGAGACG 
CGGCAGCCCC GGGCCCGGCT GCTACTGACG GCGGCCCACC TGCTCTTTGT GGCCCCCCAG 
CACAACCAGT CGGAGGCCAC AGGGTCCACC AGTGGCCAGG CGCTCTTCGC CAGCAACGTG 
AAGCCTGGCC AACGTGTCTA TGTGCTGGGC GAGGGCGGGC AGCAGCTGCT GCCGGCGTCT 
GTCCACAGCG TCTCATTGCG GGAGGAGGCG TCCGGAGCCT ACGCCCCACT CACCGCCCAG 
GGCACCATCC TCATCAACCG GGTGTTGGCC TCCTGCTACG CCGTCATCGA GGAGCACAGT 
TGGGCCCATT GGGCCTTCGC ACCATTCCGC TTGGCTCAGG GGCTGCTGGC CGCCCTCTGC 
CCAGATGGGG CCATCCCTAC TGCCGCCACC ACCACCACTG GCATCCATTG GTACTCACGG 
CTCCTCTACC GCATCGGCAG CTGGGTGCTG GATGGTGACG CGCTGCATCC GCTGGGCATG 
GTGGCACCGG CCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990152.1
CDS	211..1488
Translation	MVEMLLLTRILLVGFICALLVSSGLTCGPGRGIGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKITRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGGCFPGSATVHLEHGGTKLVKDLSPGDRVLAADADGRLLYSDFLTFLDRMDSSRKLFYVIETRQPRARLLLTAAHLLFVAPQHNQSEATGSTSGQALFASNVKPGQRVYVLGEGGQQLLPASVHSVSLREEASGAYAPLTAQGTILINRVLASCYAVIEEHSWAHWAFAPFRLAQGLLAALCPDGAIPTAATTTTGIHWYSRLLYRIGSWVLDGDALHPLGMVAPAS

Target ORF information:

RefSeq Version	NM_204821.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus sonic hedgehog (SHH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204821.1

ORF Insert Sequence:

ATGGTCGAAA TGCTGCTGTT GACAAGAATT CTCTTGGTGG GCTTCATCTG CGCTCTTTTA 
GTCTCCTCTG GGCTGACTTG TGGACCAGGC AGGGGCATTG GAAAAAGGAG GCACCCCAAA 
AAGCTGACCC CGTTAGCCTA TAAGCAGTTT ATTCCCAATG TGGCAGAGAA GACCCTAGGG 
GCCAGTGGAA GATATGAAGG GAAGATCACA AGAAACTCCG AGAGATTTAA AGAACTAACC 
CCAAATTACA ACCCTGACAT TATTTTTAAG GATGAAGAGA ACACGGGAGC TGACAGACTG 
ATGACTCAGC GCTGCAAGGA CAAGCTGAAT GCCCTGGCGA TCTCGGTGAT GAACCAGTGG 
CCCGGGGTGA AGCTGCGGGT GACCGAGGGC TGGGACGAGG ATGGCCATCA CTCCGAGGAA 
TCGCTGCACT ACGAGGGTCG CGCCGTGGAC ATCACCACGT CGGATCGGGA CCGCAGCAAG 
TACGGAATGC TGGCCCGCCT CGCCGTCGAG GCCGGCTTCG ACTGGGTCTA CTACGAGTCC 
AAGGCGCACA TCCACTGCTC CGTCAAAGCA GAAAACTCAG TGGCAGCGAA ATCAGGAGGC 
TGCTTCCCTG GCTCAGCCAC AGTGCACCTG GAGCATGGAG GCACCAAGCT GGTGAAGGAC 
CTGAGCCCTG GGGACCGCGT GCTGGCTGCT GACGCGGACG GCCGGCTGCT CTACAGTGAC 
TTCCTCACCT TCCTCGACCG GATGGACAGC TCCCGAAAGC TCTTCTACGT CATCGAGACG 
CGGCAGCCCC GGGCCCGGCT GCTACTGACG GCGGCCCACC TGCTCTTTGT GGCCCCCCAG 
CACAACCAGT CGGAGGCCAC AGGGTCCACC AGTGGCCAGG CGCTCTTCGC CAGCAACGTG 
AAGCCTGGCC AACGTGTCTA TGTGCTGGGC GAGGGCGGGC AGCAGCTGCT GCCGGCGTCT 
GTCCACAGCG TCTCATTGCG GGAGGAGGCG TCCGGAGCCT ACGCCCCACT CACCGCCCAG 
GGCACCATCC TCATCAACCG GGTGTTGGCC TCCTGCTACG CCGTCATCGA GGAGCACAGT 
TGGGCCCATT GGGCCTTCGC ACCATTCCGC TTGGCTCAGG GGCTGCTGGC CGCCCTCTGC 
CCAGATGGGG CCATCCCTAC TGCCGCCACC ACCACCACTG GCATCCATTG GTACTCACGG 
CTCCTCTACC GCATCGGCAG CTGGGTGCTG GATGGTGACG CGCTGCATCC GCTGGGCATG 
GTGGCACCGG CCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Upstream regulation for initiation of restricted Shh expression in the chick limb bud.
Developmental dynamics : an official publication of the American Association of Anatomists246(5)417-430(2017 May)
Matsubara H,Saito D,Abe G,Yokoyama H,Suzuki T,Tamura K

SHH ventralizes the otocyst by maintaining basal PKA activity and regulating GLI3 signaling.
Developmental biology420(1)100-109(2016 Dec)
Ohta S,Wang B,Mansour SL,Schoenwolf GC

Splenic gene expression profiling in White Leghorn layer inoculated with the Salmonella enterica serovar Enteritidis.
Animal genetics46(6)617-26(2015 Dec)
Wu G,Liu L,Qi Y,Sun Y,Yang N,Xu G,Zhou H,Li X

Sonic hedgehog overexpression regulates the neuroepithelial cells proliferation in the spinal cord of dorsal regions during chicken embryo development.
Neuro endocrinology letters36(4)380-6(2015)
Yang C,Li X,Liu Y,Li H,Guo Z,Lin J

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SHH cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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