TOP2A cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	TOP2A
Entrez Gene ID	395570
Full Name	topoisomerase (DNA) II alpha
General protein information	Preferred Names topoisomerase (DNA) II alpha Names DNA topoisomerase 2-alpha DNA topoisomerase II, alpha isozyme DNA topoisomeraseII_alpha topoisomerase (DNA) II alpha 170kDa
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_204791.1	NP_990122.1	DNA topoisomerase 2-alpha

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Top2a	NP_035753.2
Rattus norvegicus (Norway rat)	Top2a	NP_071519.2
Xenopus tropicalis (tropical clawed frog)	topoii	XP_002942632.2
Danio rerio (zebrafish)	top2a	NP_001003834.1
Drosophila melanogaster (fruit fly)	Top2	NP_001260580.1
Caenorhabditis elegans (roundworm)	top-2	NP_496536.1
Bos taurus (cattle)	TOP2A	XP_002696055.2
Gallus gallus (chicken)	TOP2A	NP_990122.1
Homo sapiens (human)	TOP2A	NP_001058.2
Pan troglodytes (chimpanzee)	TOP2A	XP_003315524.1
Canis lupus familiaris (dog)	TOP2A	XP_537646.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008917	XP_319665.4
	TOP2A	XP_002808175.1

Gene Ontology
Bibliography

Related articles in PubMed

Mre11 Is Essential for the Removal of Lethal Topoisomerase 2 Covalent Cleavage Complexes.
Hoa NN, Shimizu T, Zhou ZW, Wang ZQ, Deshpande RA, Paull TT, Akter S, Tsuda M, Furuta R, Tsutsui K, Takeda S, Sasanuma H
Molecular cell64(3)580-592(2016 Nov)

PICH promotes sister chromatid disjunction and co-operates with topoisomerase II in mitosis.
Nielsen CF, Huttner D, Bizard AH, Hirano S, Li TN, Palmai-Pallag T, Bjerregaard VA, Liu Y, Nigg EA, Wang LH, Hickson ID
Nature communications68962(2015 Dec)

Mitotic chromosomes are compacted laterally by KIF4 and condensin and axially by topoisomerase II?.
Samejima K, Samejima I, Vagnarelli P, Ogawa H, Vargiu G, Kelly DA, de Lima Alves F, Kerr A, Green LC, Hudson DF, Ohta S, Cooke CA, Farr CJ, Rappsilber J, Earnshaw WC
The Journal of cell biology199(5)755-70(2012 Nov)

Maternal topoisomerase II alpha, not topoisomerase II beta, enables embryonic development of zebrafish top2a-/- mutants.
Sapetto-Rebow B, McLoughlin SC, O'Shea LC, O'Leary O, Willer JR, Alvarez Y, Collery R, O'Sullivan J, Van Eeden F, Hensey C, Kennedy BN
BMC developmental biology1171(2011 Nov)

Co-localization of chicken DNA topoisomerase IIalpha, but not beta, with sites of DNA replication and possible involvement of a C-terminal region of alpha through its binding to PCNA.
Niimi A, Suka N, Harata M, Kikuchi A, Mizuno S
Chromosoma110(2)102-14(2001 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Both the genome instability and cell death of MRE11-null and MRE11-mutated H129N cells are significantly reversed by overexpression of Tdp2, an enzyme that eliminates covalent Top2 conjugates; thus, the essential role of Mre11 nuclease activity is likely to remove the DNA lesions.
Title: Mre11 Is Essential for the Removal of Lethal Topoisomerase 2 Covalent Cleavage Complexes.

PICH and Topo II cooperate to prevent chromosome missegregation events in mitosis.
Title: PICH promotes sister chromatid disjunction and co-operates with topoisomerase II in mitosis.

The topoisomerase IIalpha contributed to shaping mitotic chromosomes by promoting the shortening of the chromatid axes and apparently acting in opposition to the actions of KIF4 and condensins.
Title: Mitotic chromosomes are compacted laterally by KIF4 and condensin and axially by topoisomerase II?.

found in chicken chromosome scaffolds. Chicken topoisomerase II is located in the neucleus, associated with mitotic chromosomes and chromosome scaffolds.
Title: Topoisomerase II is a structural component of mitotic chromosome scaffolds.

The following TOP2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa28074	NM_204791.1 Latest version!	Gallus gallus topoisomerase (DNA) II alpha (TOP2A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOP2A ( NM_204791.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990122.1 Gallus gallus topoisomerase (DNA) II alpha (TOP2A), mRNA.

Price & Availability↑

CloneID	OGa28074
Clone ID Related Accession (Same CDS sequence)	NM_204791.1
Accession Version	NM_204791.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Gallus gallus(chicken)
Product	DNA topoisomerase 2-alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB007445.2. ##Evidence-Data-START## Transcript exon combination :: AB007445.2 [ECO:0000332] ##Evidence-Data-END##

ATGGAGCTCC TGGACTCCCC GGCGCCGCTC CGACCCCTGC ACGACAACCC CCGGCTGCCC 
AAAGCCGATG GCGCCCAGAA GCGGCTCTCG GTGGAGCGCA TCTACCAGAA GAAGACGCAG 
CTGGAGCACA TCCTCCTGCG GCCCGACACC TACATCGGCT CCGTGGAGAC CGTCACGCAG 
CAAATGTGGG TGTTCGATGA GGACGTGGGC CTCAACTGCA GGGATGTGAC CTTCGTGCCC 
GGCCTGTACA AGATCTTCGA CGAGATCCTG GTCAATGCTG CGGACAACAA GCAGAGGGAT 
AAAAGCATGT CCTGCATCAA AGTTACAATC GACCCAGAGA ATAACACCAT CAGTGTGTGG 
AACAATGGGA AAGGCATTCC AGTCGTTGAA CATAAAGTGG AAAAGGTCTA TGTGCCAGCA 
CTCATCTTTG GACAGCTGCT GACATCCAGC AACTATGATG ACGATGAAAA GAAAGTCACA 
GGTGGGCGAA ATGGTTACGG AGCCAAACTG TGCAACATAT TCAGCACCAA ATTTACTGTG 
GAGACTGCGT GTCGTGAATA CAAAAAGTTG TTCAAGCAGA CCTGGACAGA CAACATGGGG 
AAGGCCGGTG AGATGACGCT GAAGCATTTT GATGGAGAAG ATTACACGTG TGTCACTTTC 
CAACCTGATC TGTCGAAATT CAAAATGACG ATTCTCGACA AGGACATTGT AGCACTGATG 
TCTCGGAGGG CGTATGACAT TGCTGGATCC ACAAAGGATG TCAAAGTTTT CCTGAATGGG 
AAGAGGCTGC CTGTGAAAGG ATTTCGCAGC TACGTGGACC TCTATCTGAA GGACAAAGTG 
GATGAGACTG GAAATGCACT TAAGGTTATT CATGAGGAAG TGAATTCCAG GTGGGAAGTG 
TGCTTGACTT TAAGTGAAAA AGGCTTCCAG CAAGTTAGCT TTGTCAACAG CATTGCCACC 
ACAAAGGGTG GCAGGCACGT TGATTACGTA GCTGACCAGA TTGTGACTAA ACTCATTGAT 
GTTGTGAAAA AGAAGAACAA AAACGGAGTT GGAGTGAAGC CGTTTCAGGT GAAGAATCAC 
ATGTGGATTT TTGTGAATTC CTTGATTGAA AACCCAACCT TTGACTCTCA GACTAAAGAG 
AACATGACTC TGCAGGCAAA GAGCTTTGGC TCAACATGTA AACTGAGTGA GAAATTTATC 
AAGGGTGCAG TTGGCTGTGG CATTGTTGAA AGTATCCTAA ACTGGGTAAA ATTTAAAGCT 
CAGACCCAGC TGAATAAGAA ATGTTCAGCT GTGAAGCACA CCAAGATTAA GGGCGTTCCT 
AAACTGGATG ATGCCAATGA TGCTGGCAGC AAGAATTCAA TAGATTGTAC ACTCATCCTG 
ACTGAGGGAG ACTCAGCCAA AACGCTGGCT GTCTCTGGTT TAGGAGTGGT TGGTAGAGAC 
AAATACGGAG TATTTCCCCT TCGTGGGAAA ATGCTCAACG TCAGGGAAGC TTCTCATAAG 
CAGATAATGG AAAATGCTGA AATCAACAAC ATCATCAAGA TTGTGGGTTT ACAGTATAAG 
AAGAATTACG AAGATCGAGA ATCTTTAAAG AGTCTTCGCT ATGGGAAGAT CATGATTATG 
ACAGATCAGG ACCAAGATGG ATCTCATATC AAAGGTTTGC TGATTAACTT CATCCATCAC 
AACTGGCCTT CTCTTCTAAG ACACAACTTC TTGGAGGAAT TTATTACCCC CATCATAAAG 
GTCTCTAAAA ACAAAGAAGA AATTCCATTC TATAGCATTC CTGAGTTTGA AGAATGGAAA 
AGCAGTACAC AGAACTACAA CTCATGGAAA ATCAAGTACT ACAAAGGTTT GGGTACCAGC 
ACATCGAAGG AGGCTAAGGA GTACTTTGCA GATATGGCTA GACATCGAAT TGGCTTCAAG 
TACTCTGGTC CTGAAGATGA TGCTGCCATC ACCCTGGCCT TTAGTAAGAA GAAGGTAGAA 
GAGCGAAAGG AATGGCTGAC TAATTTCATG GAGGACAGAA GACAGCGGAA CGTGCATGGG 
CTGCCAGAGG ACTACCTGTA TGGGAAAGAT ACTAATTACC TGACATACAA CGACTTCATC 
AACAAGGAGT TGGTCCTGTT CTCAAACTCA GACAATGAAA GATCGATCCC ATCTCTGGTT 
GATGGTTTGA AGCCAGGCCA GAGGAAAGTT CTGTTCACGT GTTTCAAAAG AAACGACAAA 
CGGGAGGTGA AAGGTGCTCA GCTGGCTGGT TCTGTAGCTG AGATGTCCTC GTACCATCAC 
GGTGAGGCAT CACTGATGAT GACGATTATA AACCTAGCTC AGAACTTTGT GGGCAGTAAC 
AACCTCAACC TGCTGCAGCC CATCGGTCAG TTTGGCACAA GGCTGCATGG TGGGAAGGAC 
TCTGCAAGCC CTCGATACAT CTTTACAATG CTAAGCCCAC TAGCACGGTT GCTGTTCCCA 
CCAGTGGATG ACAATGTCCT GAGGTTCCTC TATGATGACA ACCAACGTGT GGAGCCGGAG 
TGGTACATGC CTATAATTCC GATGGTGCTG ATAAATGGGG CAGAAGGGAT TGGCACCGGC 
TGGTCCTGTA AGATCCCCAA CTTTGATATC AGGGAGACTG TGAATAACAT CCGTTGTCTG 
TTGGATGGAA AAGAACCACT GCCAATGCTT CCCAGCTACA AGAACTTCAA AGGCACAATA 
GATGAGCTTG GACCTAACCA GTATGTGATA AGTGGTGAAG TGTCTATCCT TGATTCTACG 
ACCATTGAAA TCACTGAGCT GCCCGTCAGA ACATGGACAC AGACCTACAA AGAGCAAGTT 
CTGGAGCCAA TGCTAAATGG GACTGAGAAG ACCCCCCCAC TAATCACAGA CTATAAAGAA 
TACCACACTG ACACCACAGT GAAGTTTGTA GTGAAGATGA GTGAGGAAAA ACTAGCTGAA 
GCTGAAGCAG TGGGGTTGCA TAAGGTCTTC AAACTCCAAA CAAATCTAAC ATGCAACTCT 
ATGGTTCTTT TTGACCACGT TGGCTTTCTC AAGAAGTACG AGTCTCCCCA GGATATCCTT 
AAAGAGTTCT TTGAACTCAG GCTCCGATAC TATGGTTTGA GGAAAGAATG GCTTATTGGG 
ATGCTAGGAG CTGAGTCTGC AAAGCTGAAC AACCAGGCTC GATTCATCCT GGAGAAAATA 
GATGGCAAAA TTGTGATCGA AAACAAACCC AAGAAAGAAC TGATCCAAGT TCTTATCCAA 
AGAGGATATG AATCAGATCC AGTGAAGGCC TGGAAGGAAT TGCAAAACAA GGAGGAAGAA 
GAAGGAGATG AGAGTGGTGA AGAATCAGCT GCAGCTACCG GACCAGATTT CAACTACCTT 
CTGAACATGC CCCTTTGGTA CCTGACTAAG GAGAAGAAAG ATGAGCTCTG CAAGCAGAGA 
GATAACAAAG ACAAGGAGCT GGAAGACCTG AAGCACAAGA GCCCCTCTGA CCTGTGGAAA 
GAAGACCTTG CTGCCTTTGT TGAAGAGCTT GATGCAGTAG AAGCCAAGCA AATGCAGGAT 
GAGATGGCAG GGATAACTGG CAAACCTCTA AAGGTGAAAG GAGGGAAGCA AGGAGGGAAG 
CAGAAGGTGA CAAAGGCACA GCTGGCGGAA GTAATGCCGT CACCTCATGG CATAAGGGTT 
GTTCCTCGGG TCACTGCAGA AATGAAGGCG GAGGCAGAAA AGAGAATTAA AAAGAAGATA 
AAGAGTGAAA AAAATGAATC TGATGAGAAA CAAGAAGGAA ACTCATCGGG GGATAAAGAG 
CCCTCAAGCC TTAAGCAACG GTTAGCACAG AAGCGTAAAG CCGAGCAAGG CACCAAGAGG 
CAGACCACTC TACCATTTAA ACCAATAAAG AAAATGAAGC GCAACCCTTG GTCTGACTCA 
GAGTCTGACT CAGAGTCGGA TGATTTTGAA GTGCCTTCTA AAAGAGAGCG AGTGGTTCGC 
CAGGCAGCAG CCAAAATCAA GCCCATGGTC AATTCGGACT CGGATGCAGA TCTGACCAGC 
TCAGATGAGG ACTCTGAGTA TCAGGAGAAC AGTGAAGGCA ACACCGACTC AGATACAACC 
TCAAAAAAGA AACCTCCCAA GGCCAAAGCT GTCCCAAAGG AAAAAAAGGG TAAAGCTCCC 
AAGGAAAAGC CGCTGCCAGA TGCTGTCCCT GTTCGAGTCC AAAATGTTGC TGCTGAAAGT 
GCGAGTCAGG ATCCCGCGGC TCCTCCTGTG TCCGTGCCTC GTGCTCAGGC TGTGCCTAAG 
AAGCCTGCTG CTGCTAAGAA GGGCAGCACT GCCAAGGATA ACCAGCCGTC CATCATGGAT 
ATTCTCACCA AGAAGAAGGC AGCACCAAAG GCCCCCAGGC GAGCACAGAG GGAGGAGTCC 
CCACCTTCTG AAGCCACAGC GGCGGTTGCC AAGAAGCCCG GTCCCCCCCG GGGCCGAAAG 
GCCACCAAAA GGCTGACGAG TTCTTCTGAT TCTGACTCAG ATTTCGGCTC AAGGCCTTCT 
AAATCCGTTG CAGCAAAGAA ATCCAAGCGG GACGACGATG ACAGCTACAG CATTGATCTG 
ACTGCAGACA GCCCTGCAGC AGCTGCGCCC CGCACCCGGC CTGGGCGCCT CAAAAAGCCC 
GTCCAGTACT TGGAGTCATC TGATGAGGAT GATATGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990122.1
CDS	55..4716
Translation	MELLDSPAPLRPLHDNPRLPKADGAQKRLSVERIYQKKTQLEHILLRPDTYIGSVETVTQQMWVFDEDVGLNCRDVTFVPGLYKIFDEILVNAADNKQRDKSMSCIKVTIDPENNTISVWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACREYKKLFKQTWTDNMGKAGEMTLKHFDGEDYTCVTFQPDLSKFKMTILDKDIVALMSRRAYDIAGSTKDVKVFLNGKRLPVKGFRSYVDLYLKDKVDETGNALKVIHEEVNSRWEVCLTLSEKGFQQVSFVNSIATTKGGRHVDYVADQIVTKLIDVVKKKNKNGVGVKPFQVKNHMWIFVNSLIENPTFDSQTKENMTLQAKSFGSTCKLSEKFIKGAVGCGIVESILNWVKFKAQTQLNKKCSAVKHTKIKGVPKLDDANDAGSKNSIDCTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKMLNVREASHKQIMENAEINNIIKIVGLQYKKNYEDRESLKSLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHNFLEEFITPIIKVSKNKEEIPFYSIPEFEEWKSSTQNYNSWKIKYYKGLGTSTSKEAKEYFADMARHRIGFKYSGPEDDAAITLAFSKKKVEERKEWLTNFMEDRRQRNVHGLPEDYLYGKDTNYLTYNDFINKELVLFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKGAQLAGSVAEMSSYHHGEASLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPVDDNVLRFLYDDNQRVEPEWYMPIIPMVLINGAEGIGTGWSCKIPNFDIRETVNNIRCLLDGKEPLPMLPSYKNFKGTIDELGPNQYVISGEVSILDSTTIEITELPVRTWTQTYKEQVLEPMLNGTEKTPPLITDYKEYHTDTTVKFVVKMSEEKLAEAEAVGLHKVFKLQTNLTCNSMVLFDHVGFLKKYESPQDILKEFFELRLRYYGLRKEWLIGMLGAESAKLNNQARFILEKIDGKIVIENKPKKELIQVLIQRGYESDPVKAWKELQNKEEEEGDESGEESAAATGPDFNYLLNMPLWYLTKEKKDELCKQRDNKDKELEDLKHKSPSDLWKEDLAAFVEELDAVEAKQMQDEMAGITGKPLKVKGGKQGGKQKVTKAQLAEVMPSPHGIRVVPRVTAEMKAEAEKRIKKKIKSEKNESDEKQEGNSSGDKEPSSLKQRLAQKRKAEQGTKRQTTLPFKPIKKMKRNPWSDSESDSESDDFEVPSKRERVVRQAAAKIKPMVNSDSDADLTSSDEDSEYQENSEGNTDSDTTSKKKPPKAKAVPKEKKGKAPKEKPLPDAVPVRVQNVAAESASQDPAAPPVSVPRAQAVPKKPAAAKKGSTAKDNQPSIMDILTKKKAAPKAPRRAQREESPPSEATAAVAKKPGPPRGRKATKRLTSSSDSDSDFGSRPSKSVAAKKSKRDDDDSYSIDLTADSPAAAAPRTRPGRLKKPVQYLESSDEDDMF

Target ORF information:

RefSeq Version	NM_204791.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus topoisomerase (DNA) II alpha (TOP2A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204791.1

ORF Insert Sequence:

ATGGAGCTCC TGGACTCCCC GGCGCCGCTC CGACCCCTGC ACGACAACCC CCGGCTGCCC 
AAAGCCGATG GCGCCCAGAA GCGGCTCTCG GTGGAGCGCA TCTACCAGAA GAAGACGCAG 
CTGGAGCACA TCCTCCTGCG GCCCGACACC TACATCGGCT CCGTGGAGAC CGTCACGCAG 
CAAATGTGGG TGTTCGATGA GGACGTGGGC CTCAACTGCA GGGATGTGAC CTTCGTGCCC 
GGCCTGTACA AGATCTTCGA CGAGATCCTG GTCAATGCTG CGGACAACAA GCAGAGGGAT 
AAAAGCATGT CCTGCATCAA AGTTACAATC GACCCAGAGA ATAACACCAT CAGTGTGTGG 
AACAATGGGA AAGGCATTCC AGTCGTTGAA CATAAAGTGG AAAAGGTCTA TGTGCCAGCA 
CTCATCTTTG GACAGCTGCT GACATCCAGC AACTATGATG ACGATGAAAA GAAAGTCACA 
GGTGGGCGAA ATGGTTACGG AGCCAAACTG TGCAACATAT TCAGCACCAA ATTTACTGTG 
GAGACTGCGT GTCGTGAATA CAAAAAGTTG TTCAAGCAGA CCTGGACAGA CAACATGGGG 
AAGGCCGGTG AGATGACGCT GAAGCATTTT GATGGAGAAG ATTACACGTG TGTCACTTTC 
CAACCTGATC TGTCGAAATT CAAAATGACG ATTCTCGACA AGGACATTGT AGCACTGATG 
TCTCGGAGGG CGTATGACAT TGCTGGATCC ACAAAGGATG TCAAAGTTTT CCTGAATGGG 
AAGAGGCTGC CTGTGAAAGG ATTTCGCAGC TACGTGGACC TCTATCTGAA GGACAAAGTG 
GATGAGACTG GAAATGCACT TAAGGTTATT CATGAGGAAG TGAATTCCAG GTGGGAAGTG 
TGCTTGACTT TAAGTGAAAA AGGCTTCCAG CAAGTTAGCT TTGTCAACAG CATTGCCACC 
ACAAAGGGTG GCAGGCACGT TGATTACGTA GCTGACCAGA TTGTGACTAA ACTCATTGAT 
GTTGTGAAAA AGAAGAACAA AAACGGAGTT GGAGTGAAGC CGTTTCAGGT GAAGAATCAC 
ATGTGGATTT TTGTGAATTC CTTGATTGAA AACCCAACCT TTGACTCTCA GACTAAAGAG 
AACATGACTC TGCAGGCAAA GAGCTTTGGC TCAACATGTA AACTGAGTGA GAAATTTATC 
AAGGGTGCAG TTGGCTGTGG CATTGTTGAA AGTATCCTAA ACTGGGTAAA ATTTAAAGCT 
CAGACCCAGC TGAATAAGAA ATGTTCAGCT GTGAAGCACA CCAAGATTAA GGGCGTTCCT 
AAACTGGATG ATGCCAATGA TGCTGGCAGC AAGAATTCAA TAGATTGTAC ACTCATCCTG 
ACTGAGGGAG ACTCAGCCAA AACGCTGGCT GTCTCTGGTT TAGGAGTGGT TGGTAGAGAC 
AAATACGGAG TATTTCCCCT TCGTGGGAAA ATGCTCAACG TCAGGGAAGC TTCTCATAAG 
CAGATAATGG AAAATGCTGA AATCAACAAC ATCATCAAGA TTGTGGGTTT ACAGTATAAG 
AAGAATTACG AAGATCGAGA ATCTTTAAAG AGTCTTCGCT ATGGGAAGAT CATGATTATG 
ACAGATCAGG ACCAAGATGG ATCTCATATC AAAGGTTTGC TGATTAACTT CATCCATCAC 
AACTGGCCTT CTCTTCTAAG ACACAACTTC TTGGAGGAAT TTATTACCCC CATCATAAAG 
GTCTCTAAAA ACAAAGAAGA AATTCCATTC TATAGCATTC CTGAGTTTGA AGAATGGAAA 
AGCAGTACAC AGAACTACAA CTCATGGAAA ATCAAGTACT ACAAAGGTTT GGGTACCAGC 
ACATCGAAGG AGGCTAAGGA GTACTTTGCA GATATGGCTA GACATCGAAT TGGCTTCAAG 
TACTCTGGTC CTGAAGATGA TGCTGCCATC ACCCTGGCCT TTAGTAAGAA GAAGGTAGAA 
GAGCGAAAGG AATGGCTGAC TAATTTCATG GAGGACAGAA GACAGCGGAA CGTGCATGGG 
CTGCCAGAGG ACTACCTGTA TGGGAAAGAT ACTAATTACC TGACATACAA CGACTTCATC 
AACAAGGAGT TGGTCCTGTT CTCAAACTCA GACAATGAAA GATCGATCCC ATCTCTGGTT 
GATGGTTTGA AGCCAGGCCA GAGGAAAGTT CTGTTCACGT GTTTCAAAAG AAACGACAAA 
CGGGAGGTGA AAGGTGCTCA GCTGGCTGGT TCTGTAGCTG AGATGTCCTC GTACCATCAC 
GGTGAGGCAT CACTGATGAT GACGATTATA AACCTAGCTC AGAACTTTGT GGGCAGTAAC 
AACCTCAACC TGCTGCAGCC CATCGGTCAG TTTGGCACAA GGCTGCATGG TGGGAAGGAC 
TCTGCAAGCC CTCGATACAT CTTTACAATG CTAAGCCCAC TAGCACGGTT GCTGTTCCCA 
CCAGTGGATG ACAATGTCCT GAGGTTCCTC TATGATGACA ACCAACGTGT GGAGCCGGAG 
TGGTACATGC CTATAATTCC GATGGTGCTG ATAAATGGGG CAGAAGGGAT TGGCACCGGC 
TGGTCCTGTA AGATCCCCAA CTTTGATATC AGGGAGACTG TGAATAACAT CCGTTGTCTG 
TTGGATGGAA AAGAACCACT GCCAATGCTT CCCAGCTACA AGAACTTCAA AGGCACAATA 
GATGAGCTTG GACCTAACCA GTATGTGATA AGTGGTGAAG TGTCTATCCT TGATTCTACG 
ACCATTGAAA TCACTGAGCT GCCCGTCAGA ACATGGACAC AGACCTACAA AGAGCAAGTT 
CTGGAGCCAA TGCTAAATGG GACTGAGAAG ACCCCCCCAC TAATCACAGA CTATAAAGAA 
TACCACACTG ACACCACAGT GAAGTTTGTA GTGAAGATGA GTGAGGAAAA ACTAGCTGAA 
GCTGAAGCAG TGGGGTTGCA TAAGGTCTTC AAACTCCAAA CAAATCTAAC ATGCAACTCT 
ATGGTTCTTT TTGACCACGT TGGCTTTCTC AAGAAGTACG AGTCTCCCCA GGATATCCTT 
AAAGAGTTCT TTGAACTCAG GCTCCGATAC TATGGTTTGA GGAAAGAATG GCTTATTGGG 
ATGCTAGGAG CTGAGTCTGC AAAGCTGAAC AACCAGGCTC GATTCATCCT GGAGAAAATA 
GATGGCAAAA TTGTGATCGA AAACAAACCC AAGAAAGAAC TGATCCAAGT TCTTATCCAA 
AGAGGATATG AATCAGATCC AGTGAAGGCC TGGAAGGAAT TGCAAAACAA GGAGGAAGAA 
GAAGGAGATG AGAGTGGTGA AGAATCAGCT GCAGCTACCG GACCAGATTT CAACTACCTT 
CTGAACATGC CCCTTTGGTA CCTGACTAAG GAGAAGAAAG ATGAGCTCTG CAAGCAGAGA 
GATAACAAAG ACAAGGAGCT GGAAGACCTG AAGCACAAGA GCCCCTCTGA CCTGTGGAAA 
GAAGACCTTG CTGCCTTTGT TGAAGAGCTT GATGCAGTAG AAGCCAAGCA AATGCAGGAT 
GAGATGGCAG GGATAACTGG CAAACCTCTA AAGGTGAAAG GAGGGAAGCA AGGAGGGAAG 
CAGAAGGTGA CAAAGGCACA GCTGGCGGAA GTAATGCCGT CACCTCATGG CATAAGGGTT 
GTTCCTCGGG TCACTGCAGA AATGAAGGCG GAGGCAGAAA AGAGAATTAA AAAGAAGATA 
AAGAGTGAAA AAAATGAATC TGATGAGAAA CAAGAAGGAA ACTCATCGGG GGATAAAGAG 
CCCTCAAGCC TTAAGCAACG GTTAGCACAG AAGCGTAAAG CCGAGCAAGG CACCAAGAGG 
CAGACCACTC TACCATTTAA ACCAATAAAG AAAATGAAGC GCAACCCTTG GTCTGACTCA 
GAGTCTGACT CAGAGTCGGA TGATTTTGAA GTGCCTTCTA AAAGAGAGCG AGTGGTTCGC 
CAGGCAGCAG CCAAAATCAA GCCCATGGTC AATTCGGACT CGGATGCAGA TCTGACCAGC 
TCAGATGAGG ACTCTGAGTA TCAGGAGAAC AGTGAAGGCA ACACCGACTC AGATACAACC 
TCAAAAAAGA AACCTCCCAA GGCCAAAGCT GTCCCAAAGG AAAAAAAGGG TAAAGCTCCC 
AAGGAAAAGC CGCTGCCAGA TGCTGTCCCT GTTCGAGTCC AAAATGTTGC TGCTGAAAGT 
GCGAGTCAGG ATCCCGCGGC TCCTCCTGTG TCCGTGCCTC GTGCTCAGGC TGTGCCTAAG 
AAGCCTGCTG CTGCTAAGAA GGGCAGCACT GCCAAGGATA ACCAGCCGTC CATCATGGAT 
ATTCTCACCA AGAAGAAGGC AGCACCAAAG GCCCCCAGGC GAGCACAGAG GGAGGAGTCC 
CCACCTTCTG AAGCCACAGC GGCGGTTGCC AAGAAGCCCG GTCCCCCCCG GGGCCGAAAG 
GCCACCAAAA GGCTGACGAG TTCTTCTGAT TCTGACTCAG ATTTCGGCTC AAGGCCTTCT 
AAATCCGTTG CAGCAAAGAA ATCCAAGCGG GACGACGATG ACAGCTACAG CATTGATCTG 
ACTGCAGACA GCCCTGCAGC AGCTGCGCCC CGCACCCGGC CTGGGCGCCT CAAAAAGCCC 
GTCCAGTACT TGGAGTCATC TGATGAGGAT GATATGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mre11 Is Essential for the Removal of Lethal Topoisomerase 2 Covalent Cleavage Complexes.
Molecular cell64(3)580-592(2016 Nov)
Hoa NN,Shimizu T,Zhou ZW,Wang ZQ,Deshpande RA,Paull TT,Akter S,Tsuda M,Furuta R,Tsutsui K,Takeda S,Sasanuma H

PICH promotes sister chromatid disjunction and co-operates with topoisomerase II in mitosis.
Nature communications68962(2015 Dec)
Nielsen CF,Huttner D,Bizard AH,Hirano S,Li TN,Palmai-Pallag T,Bjerregaard VA,Liu Y,Nigg EA,Wang LH,Hickson ID

Mitotic chromosomes are compacted laterally by KIF4 and condensin and axially by topoisomerase II?.
The Journal of cell biology199(5)755-70(2012 Nov)
Samejima K,Samejima I,Vagnarelli P,Ogawa H,Vargiu G,Kelly DA,de Lima Alves F,Kerr A,Green LC,Hudson DF,Ohta S,Cooke CA,Farr CJ,Rappsilber J,Earnshaw WC

Maternal topoisomerase II alpha, not topoisomerase II beta, enables embryonic development of zebrafish top2a-/- mutants.
BMC developmental biology1171(2011 Nov)
Sapetto-Rebow B,McLoughlin SC,O'Shea LC,O'Leary O,Willer JR,Alvarez Y,Collery R,O'Sullivan J,Van Eeden F,Hensey C,Kennedy BN

Co-localization of chicken DNA topoisomerase IIalpha, but not beta, with sites of DNA replication and possible involvement of a C-terminal region of alpha through its binding to PCNA.
Chromosoma110(2)102-14(2001 May)
Niimi A,Suka N,Harata M,Kikuchi A,Mizuno S

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TOP2A cDNA ORF clone, Gallus gallus(chicken)

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