COL5A1 cDNA ORF clone, Gallus gallus(chicken)

The following COL5A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL5A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa153315 NM_204790.3
Latest version!
Gallus gallus collagen type V alpha 1 chain (COL5A1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa145095 XM_015279318.2
Latest version!
Gallus gallus collagen type V alpha 1 chain (COL5A1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa28365 NM_204790.2
Latest version!
Gallus gallus collagen type V alpha 1 chain (COL5A1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OGa43341 XM_015279318.1
Latest version!
Gallus gallus collagen, type V, alpha 1 (COL5A1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa153315
    Clone ID Related Accession (Same CDS sequence) NM_204790.3
    Accession Version NM_204790.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5508bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-11
    Organism Gallus gallus(chicken)
    Product collagen alpha-1(V) chain precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000150.1. On Dec 20, 2018 this sequence version replaced NM_204790.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AF137273.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2201357, SAMEA2201358 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGATACGC ACACGCGATG GAAAAGGCGC AGTTGGATAC GGAACTGGCA GCTGCACGTC 
    GCGCTGGTGC TGCTGGGCGC CGCGGCCCTG GGCCGGGCAG CTGAACCTGC AGACCTCTTG
    AAGGTATTAG ATTTTCACAA TTTACCTGAT GGTATCACAA GAACAACAGG GTTTTGCACA
    AGCAGAAGAT CTTCAAAAGA AGCAGACGTT GCTTACAGAG TAACAAAAGA TGCTCAGCTC
    AGCGCCCCGA CAAAGCAGCT GTATCCTGCT TCCCCATTCC CAGAGGACTT CTCCATTTTA
    ACCACTGTAA AAGCGAAGAA AGGAGGTCAG TCCTTCTTGA TCTCAATTTA CAATGAGCAA
    GGGATCCAGC AAATTGGTGT GGAGATGGGT AGATCTCCTG TATTCCTGTA TGAAGACCAT
    ACAGGAAAGC CAGGCCCAGA AGACTATCCT CTCTTTAGAG GCATTAACCT AGCAGATGGC
    AAGTGGCACA GGGTCGCCAT CAGCGTGCAG AAGAAAAACG TCACCTTGAT TTTGGACTGT
    AAAAAGAAAA TAACCAAGTT CTTGGATCGA AGCGACCATC CCATCATTGA CGTCAATGGC
    ATCATCGTAT TTGGAACGAG GATATTAGAT GAGGAAGTCT TCGAGGGCGA CATCCAGCAG
    CTGCTGATCG TGGCCGACCC CCGGGCAGCC CACGACTACT GTGAGCACTA CAGCCCCGAC
    TGTGACACCG CCGTCCCCGA TGCTCCACAA TCCCAGGACC CCAACCAGGA TGAATACTAT
    ACCGATGGGG AGGGAGAAGG GGACACCTAC TACTATGAGT ACCCCTACTA TGAAGACGTT
    GATGAAGCTG TAAAGCCTGA AGCACCCACC ACCAAACCTG CACCCCCAGG AGTGGCTGCT
    GGAGAGAGAC CCGAAACCAA GCAGGACTAT CCTTCCCCCA CGCCATCCCC TGAAGCAGGG
    AACCCCAGCA GGCAGACCAA AGGGGCTGCA CCCGTGGACG ACCCCCTGGT GGATGAGTAC
    AACTATGAAA CCATCAACGA GGAGTACTTC ACACCTCTGC CCTATGAAGA CATCAACTAC
    AACGAAGAAG TAGACCCCCA GGGAGGGCTC ACTGAAAATG CAGTGGAGGC GGAGCTCCCC
    ACGAGTACCG TCATCACCTA CAATGAGACT GATGCTGCTC AAGGAGGAGA CGACCTGGAT
    AAAGACTTCA CCGAGGAAAC GATAAAAGAA TACGATGGGA ACTACTACTA TTATGACAGA
    ACAGTGTCTC CTGACATCGG GCCTGGGATG CCTGCCAACC AGGACACCAT CTACGAAGGG
    ATTGGAGGCC CACGGGGTGA GAAGGGGCAG AAGGGCGAGC CTGCCATTAT TGAGCCGGGT
    ATGCTTGTGG AAGGTCCCCC TGGTCCCGAA GGCCCCGCAG GCCTTCCAGG ACCTCCAGGA
    CCAACTGGAC CTGTGGGCTT AATGGGAGAT CCTGGAGAGA GAGGACCGCC TGGCCGCCCA
    GGTCTTCCAG GAGCAGATGG TTTACCAGGA CCACCAGGGA CAATGCTTAT GTTGCCTTTC
    CGCTTCAGTG GAGGAGGAGA TGCTGGCTCC AAAGGACCCA TGGTCTCTGC CCAGGAAGCT
    CAAGCCCAGG CCATCCTGCA GCAGGCGAGG CTGGCACTGA GAGGACCGGC GGGTCCAATG
    GGTCTGACCG GGCGGCCCGG CCCCATGGGA CCCCCAGGCA GTGGAGGATT GAAGGGCGAA
    GCTGGAGAAA TGGGACCTCA GGGTCCACGA GGCATCCAGG GCCCTCCCGG CCCGGCGGGG
    AAGCCAGGCC GCAGGGGACG AGCTGGCAGC GATGGTGCCC GGGGAATGCC AGGACAGACA
    GGACCAAAGG GTGACCGAGG GTTTGATGGC TTGGCTGGCT TGCCTGGCGA GAAGGGGAAC
    AGAGGTGAGC CAGGTCCCCA CGGCCCCCCG GGGGCACCCG GAGAGGACGG GGAGAGGGGG
    GATGATGGAG AAGTTGGACC CAGAGGGCTG CCCGGAGAAC CTGGCCCCCG AGGACTGCTG
    GGACCCAAAG GGCCACCAGG CCCACCGGGG CCACCGGGTG TGGCCGGTAT GGATGGACAA
    ACTGGTCCCA AGGGGAACGT GGGTCCCCAG GGTGAGCCGG GACCCCCAGG CCAGCAGGGC
    AACCCAGGTG CTCAGGGTCT TCCAGGTCCA CAAGGCCCCA TCGGTCCCCC AGGAGAGAAG
    GGGCCGCTGG GCAAACCTGG TCTTCCTGGC ATGCCTGGTG CAGATGGTCC TCCGGGTCAT
    CCTGGCAAGG AAGGTCCTCC TGGAGAGAAG GGGAGTCAGG GTCCACCAGG CCCTCAGGGT
    CCCATTGGTT ATCCAGGTCC ACGCGGAGTC AAGGGAGCGG ATGGTGTTCG TGGGTTGAAG
    GGCACCAAAG GTGAAAAGGG TGAAGATGGC TTCCCTGGCT TCAAAGGGGA TATGGGCATC
    AAGGGAGACC GGGGTGAAAT CGGACCTCCG GGCCCCAGGG GTGAGGATGG CCCTGAAGGT
    CCCAAAGGTC GCTCGGGTCC CAACGGAGAC CCCGGTCCTC TCGGTCCTGC TGGAGAGAAG
    GGAAAACTTG GCGTGCCAGG GCTGCCCGGC TACCCGGGCA GGCAGGGCCC AAAGGGCTCC
    ATTGGCTTCC CAGGATTTCC AGGCGCCAAC GGCGAGAAGG GCACACGGGG GACACCTGGC
    AAACCAGGTC CAAGGGGGCA ACGCGGTCCA ACGGGTCCAC GAGGGGAAAG AGGCCCTAGG
    GGAAGCACTG GGAAACCTGG CCCAAAGGGC AACTCTGGTG GTGATGGCCC CCCTGGTCCT
    CCTGGCGAAC GGGGACCACC AGGGCCTCAA GGACCAACCG GATTTCCTGG ACCAAAAGGC
    CCCCCTGGTC CTCCTGGGAA GGATGGATTG CCTGGTCACC CCGGACAGAG AGGAGAAACC
    GGTTTCCAAG GCAAGACTGG CCCACCTGGC CCCCCTGGTG TTGTAGGCCC TCAGGGCCCA
    ACTGGTGAAA CTGGTCCAAT GGGTGAGCGA GGACATCCAG GTCCTCCGGG TCCTCCGGGT
    GAACAAGGTC TTCCTGGCCT CACTGGGAAG GAAGGGACCA AGGGGGACCC CGGTCCTGCT
    GGCCTCCCAG GGAAGGATGG TCCCCCAGGC TTGCGAGGCT TCCCTGGAGA AAGAGGTCTG
    CCTGGCCCCA TTGGTTCTCC AGGGCTGAAA GGCAACGAAG GTCCCCCAGG CCCCCCTGGT
    CCTGCAGGCT CTCCTGGTGA GCGAGGTCCT GCGGGCTCAG CTGGCCCCAT TGGCTTACCA
    GGGCGACCTG GCCCACAGGG ACCTCCGGGA CCTGCAGGTG AAAAGGGAGC CCCGGGCGAG
    AAGGGTCCTC AAGGACCAGC AGGTCGGGAT GGCATCCAAG GCCCCGTGGG ACTCCCGGGT
    CCAGCAGGTC CTGTTGGCCC CCCCGGCGAG GATGGAGACA AGGGCGAGAT CGGGGAGCCT
    GGCCAGAAGG GCAGCAAGGG TGACAAAGGG GAGCAGGGTC CACCAGGTCC TACAGGCCCA
    CAAGGTCCGA TCGGTCAGCC AGGTCCAGCT GGTGCTGATG GAGAGCCAGG TCCCAGAGGG
    CAGCAAGGCC TCTTTGGTCA GAAAGGTGAT GAAGGACCTC GAGGTTTCCC CGGTCCCCCC
    GGCCCTGTGG GCTTGCAGGG CTTGCCTGGA CCTCCTGGTG AGAAGGGAGA AACAGGAGAT
    GTTGGGCAAA TGGGTCCACC AGGTCCACCT GGGCCCAGAG GCCCCTCCGG CCCACCAGGA
    GCAGACGGAC CCCAAGGCCC TGCTGGGGGA ATAGGAAATC CTGGTGCAGT AGGAGAGAAG
    GGTGAGCCTG GTGAATCTGG TGAGCCTGGT CTGCCTGGAG AGGTCGGCCT GCCGGGTCCA
    AAAGGTGAAA GAGGTGAAAA GGGTGAAGCA GGTCCCTCTG GTGCCGCTGG TCCACCTGGC
    CCTAAAGGTC CACCAGGTGA TGATGGTCCC AAAGGCAGCC CTGGTCCAGT TGGTTTCCCT
    GGTGACCCCG GTCCTCCTGG AGAGCCTGGT CCAGCTGGTC AAGATGGTCC CCCTGGTGAC
    AAAGGTGACG ACGGCGAACC TGGTCAGACA GGGTCCCCAG GTCCCACGGG AGAGCCAGGC
    CCCTCTGGAC CCCCAGGAAA AAGGGGCCCT CCTGGTCCAG CTGGTCCTGA AGGAAGGCAA
    GGAGAGAAAG GAGCCAAGGG TGAAGCTGGT TTGGAAGGGC CTCCTGGGAA GACAGGCCCA
    ATCGGTCCCC AGGGTGCTCC GGGGAAGCCC GGCCCTGATG GCCTCCGGGG AATTCCTGGT
    CCAGTTGGTG AACAAGGCCT TCCAGGATCC CCTGGCCCAG ATGGCCCCCC AGGCCCATTG
    GGCCCACCTG GTTTGCCTGG CCTCAAAGGA GACTCTGGTC CTAAAGGAGA AAAGGGTCAT
    CCAGGTTTAA TTGGTCTTAT TGGGCCGCCG GGTGAGCAGG GAGAAAAGGG TGACCGAGGT
    CTCCCGGGCC CCCAGGGCTC TGCAGGACCC AAGGGAGAGC AGGGTATCAC AGGTCCCTCT
    GGACCAATTG GACCCCCAGG GCCACCAGGA TTACCGGGTC CACCTGGTCC CAAAGGTGCT
    AAAGGATCAT CAGGTCCAAC GGGTCCAAAG GGTGAATCGG GTCTTCCTGG GCCTCCAGGG
    CCTCCTGGTC CTCCTGGAGA AGTCATCCAG CCTCTGCCCA TCCAGTCTTC CAAGAGGACC
    AGGCGAAACA TCGACGCCAG CCAGCTGGTG GACGATGGAA ACGCTGACAA CTACATGGAC
    TATGCAGATG GCATGGAGGA AATCTTTGGC TCACTGAATT CCTTGAAACT GGAAATTGAA
    CAAATGAAGC ATCCATTGGG CACTCAACAT AACCCAGCTC GTACCTGCAA GGATCTCCAG
    CTCTGTCACC CTGATTTCCC AGATGGTGAA TACTGGGTGG ATCCTAACCA AGGATGTTCC
    AGGGACTCTT TCAAAGTGTA CTGTAATTTC ACAGCTGGTG GAGAGACTTG CATCTTCCCT
    GATAAGAAGT CTGAAGGAGC TAGAATTACT TCTTGGCCAA AGGAAAACCC AGGCTCCTGG
    TTCAGTGAAT TCAAGCGCGG TAAACTGCTC TCCTATGTGG ATTCGGATGG GAACCCCATC
    GGAGTGGTAC AGATGACGTT CCTCAGGCTG CTGAGTGCCT CGGCCCACCA GAACATCACT
    TACAACTGCT ACCAGTCCGT AGCCTGGCAC GATGCCACCA CTGACAGCTA CGACAAGGCC
    ATCCGCTTCC TGGGCTCCAA CGACGAGGAG ATGTCCTACG ACAACAACCC CTACATCCGA
    GCTGCATTCG ATGGCTGCGC GGCAAAGAAG GGCTATCAGA AGACTGTCCT GGAAATCAAC
    ACGCCCAAGG TCGAGCAAGT GCCTATCGTC GACATCATGT TCAATGACTT CGGAGAAGCA
    TCACAGAAAT TTGGATTCGA GGTGGGACCA GCTTGCTTCA TGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_990121.2
    CDS1..5508
    Translation

    Target ORF information:

    RefSeq Version NM_204790.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen type V alpha 1 chain (COL5A1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_204790.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGATACGC ACACGCGATG GAAAAGGCGC AGTTGGATAC GGAACTGGCA GCTGCACGTC 
    GCGCTGGTGC TGCTGGGCGC CGCGGCCCTG GGCCGGGCAG CTGAACCTGC AGACCTCTTG
    AAGGTATTAG ATTTTCACAA TTTACCTGAT GGTATCACAA GAACAACAGG GTTTTGCACA
    AGCAGAAGAT CTTCAAAAGA AGCAGACGTT GCTTACAGAG TAACAAAAGA TGCTCAGCTC
    AGCGCCCCGA CAAAGCAGCT GTATCCTGCT TCCCCATTCC CAGAGGACTT CTCCATTTTA
    ACCACTGTAA AAGCGAAGAA AGGAGGTCAG TCCTTCTTGA TCTCAATTTA CAATGAGCAA
    GGGATCCAGC AAATTGGTGT GGAGATGGGT AGATCTCCTG TATTCCTGTA TGAAGACCAT
    ACAGGAAAGC CAGGCCCAGA AGACTATCCT CTCTTTAGAG GCATTAACCT AGCAGATGGC
    AAGTGGCACA GGGTCGCCAT CAGCGTGCAG AAGAAAAACG TCACCTTGAT TTTGGACTGT
    AAAAAGAAAA TAACCAAGTT CTTGGATCGA AGCGACCATC CCATCATTGA CGTCAATGGC
    ATCATCGTAT TTGGAACGAG GATATTAGAT GAGGAAGTCT TCGAGGGCGA CATCCAGCAG
    CTGCTGATCG TGGCCGACCC CCGGGCAGCC CACGACTACT GTGAGCACTA CAGCCCCGAC
    TGTGACACCG CCGTCCCCGA TGCTCCACAA TCCCAGGACC CCAACCAGGA TGAATACTAT
    ACCGATGGGG AGGGAGAAGG GGACACCTAC TACTATGAGT ACCCCTACTA TGAAGACGTT
    GATGAAGCTG TAAAGCCTGA AGCACCCACC ACCAAACCTG CACCCCCAGG AGTGGCTGCT
    GGAGAGAGAC CCGAAACCAA GCAGGACTAT CCTTCCCCCA CGCCATCCCC TGAAGCAGGG
    AACCCCAGCA GGCAGACCAA AGGGGCTGCA CCCGTGGACG ACCCCCTGGT GGATGAGTAC
    AACTATGAAA CCATCAACGA GGAGTACTTC ACACCTCTGC CCTATGAAGA CATCAACTAC
    AACGAAGAAG TAGACCCCCA GGGAGGGCTC ACTGAAAATG CAGTGGAGGC GGAGCTCCCC
    ACGAGTACCG TCATCACCTA CAATGAGACT GATGCTGCTC AAGGAGGAGA CGACCTGGAT
    AAAGACTTCA CCGAGGAAAC GATAAAAGAA TACGATGGGA ACTACTACTA TTATGACAGA
    ACAGTGTCTC CTGACATCGG GCCTGGGATG CCTGCCAACC AGGACACCAT CTACGAAGGG
    ATTGGAGGCC CACGGGGTGA GAAGGGGCAG AAGGGCGAGC CTGCCATTAT TGAGCCGGGT
    ATGCTTGTGG AAGGTCCCCC TGGTCCCGAA GGCCCCGCAG GCCTTCCAGG ACCTCCAGGA
    CCAACTGGAC CTGTGGGCTT AATGGGAGAT CCTGGAGAGA GAGGACCGCC TGGCCGCCCA
    GGTCTTCCAG GAGCAGATGG TTTACCAGGA CCACCAGGGA CAATGCTTAT GTTGCCTTTC
    CGCTTCAGTG GAGGAGGAGA TGCTGGCTCC AAAGGACCCA TGGTCTCTGC CCAGGAAGCT
    CAAGCCCAGG CCATCCTGCA GCAGGCGAGG CTGGCACTGA GAGGACCGGC GGGTCCAATG
    GGTCTGACCG GGCGGCCCGG CCCCATGGGA CCCCCAGGCA GTGGAGGATT GAAGGGCGAA
    GCTGGAGAAA TGGGACCTCA GGGTCCACGA GGCATCCAGG GCCCTCCCGG CCCGGCGGGG
    AAGCCAGGCC GCAGGGGACG AGCTGGCAGC GATGGTGCCC GGGGAATGCC AGGACAGACA
    GGACCAAAGG GTGACCGAGG GTTTGATGGC TTGGCTGGCT TGCCTGGCGA GAAGGGGAAC
    AGAGGTGAGC CAGGTCCCCA CGGCCCCCCG GGGGCACCCG GAGAGGACGG GGAGAGGGGG
    GATGATGGAG AAGTTGGACC CAGAGGGCTG CCCGGAGAAC CTGGCCCCCG AGGACTGCTG
    GGACCCAAAG GGCCACCAGG CCCACCGGGG CCACCGGGTG TGGCCGGTAT GGATGGACAA
    ACTGGTCCCA AGGGGAACGT GGGTCCCCAG GGTGAGCCGG GACCCCCAGG CCAGCAGGGC
    AACCCAGGTG CTCAGGGTCT TCCAGGTCCA CAAGGCCCCA TCGGTCCCCC AGGAGAGAAG
    GGGCCGCTGG GCAAACCTGG TCTTCCTGGC ATGCCTGGTG CAGATGGTCC TCCGGGTCAT
    CCTGGCAAGG AAGGTCCTCC TGGAGAGAAG GGGAGTCAGG GTCCACCAGG CCCTCAGGGT
    CCCATTGGTT ATCCAGGTCC ACGCGGAGTC AAGGGAGCGG ATGGTGTTCG TGGGTTGAAG
    GGCACCAAAG GTGAAAAGGG TGAAGATGGC TTCCCTGGCT TCAAAGGGGA TATGGGCATC
    AAGGGAGACC GGGGTGAAAT CGGACCTCCG GGCCCCAGGG GTGAGGATGG CCCTGAAGGT
    CCCAAAGGTC GCTCGGGTCC CAACGGAGAC CCCGGTCCTC TCGGTCCTGC TGGAGAGAAG
    GGAAAACTTG GCGTGCCAGG GCTGCCCGGC TACCCGGGCA GGCAGGGCCC AAAGGGCTCC
    ATTGGCTTCC CAGGATTTCC AGGCGCCAAC GGCGAGAAGG GCACACGGGG GACACCTGGC
    AAACCAGGTC CAAGGGGGCA ACGCGGTCCA ACGGGTCCAC GAGGGGAAAG AGGCCCTAGG
    GGAAGCACTG GGAAACCTGG CCCAAAGGGC AACTCTGGTG GTGATGGCCC CCCTGGTCCT
    CCTGGCGAAC GGGGACCACC AGGGCCTCAA GGACCAACCG GATTTCCTGG ACCAAAAGGC
    CCCCCTGGTC CTCCTGGGAA GGATGGATTG CCTGGTCACC CCGGACAGAG AGGAGAAACC
    GGTTTCCAAG GCAAGACTGG CCCACCTGGC CCCCCTGGTG TTGTAGGCCC TCAGGGCCCA
    ACTGGTGAAA CTGGTCCAAT GGGTGAGCGA GGACATCCAG GTCCTCCGGG TCCTCCGGGT
    GAACAAGGTC TTCCTGGCCT CACTGGGAAG GAAGGGACCA AGGGGGACCC CGGTCCTGCT
    GGCCTCCCAG GGAAGGATGG TCCCCCAGGC TTGCGAGGCT TCCCTGGAGA AAGAGGTCTG
    CCTGGCCCCA TTGGTTCTCC AGGGCTGAAA GGCAACGAAG GTCCCCCAGG CCCCCCTGGT
    CCTGCAGGCT CTCCTGGTGA GCGAGGTCCT GCGGGCTCAG CTGGCCCCAT TGGCTTACCA
    GGGCGACCTG GCCCACAGGG ACCTCCGGGA CCTGCAGGTG AAAAGGGAGC CCCGGGCGAG
    AAGGGTCCTC AAGGACCAGC AGGTCGGGAT GGCATCCAAG GCCCCGTGGG ACTCCCGGGT
    CCAGCAGGTC CTGTTGGCCC CCCCGGCGAG GATGGAGACA AGGGCGAGAT CGGGGAGCCT
    GGCCAGAAGG GCAGCAAGGG TGACAAAGGG GAGCAGGGTC CACCAGGTCC TACAGGCCCA
    CAAGGTCCGA TCGGTCAGCC AGGTCCAGCT GGTGCTGATG GAGAGCCAGG TCCCAGAGGG
    CAGCAAGGCC TCTTTGGTCA GAAAGGTGAT GAAGGACCTC GAGGTTTCCC CGGTCCCCCC
    GGCCCTGTGG GCTTGCAGGG CTTGCCTGGA CCTCCTGGTG AGAAGGGAGA AACAGGAGAT
    GTTGGGCAAA TGGGTCCACC AGGTCCACCT GGGCCCAGAG GCCCCTCCGG CCCACCAGGA
    GCAGACGGAC CCCAAGGCCC TGCTGGGGGA ATAGGAAATC CTGGTGCAGT AGGAGAGAAG
    GGTGAGCCTG GTGAATCTGG TGAGCCTGGT CTGCCTGGAG AGGTCGGCCT GCCGGGTCCA
    AAAGGTGAAA GAGGTGAAAA GGGTGAAGCA GGTCCCTCTG GTGCCGCTGG TCCACCTGGC
    CCTAAAGGTC CACCAGGTGA TGATGGTCCC AAAGGCAGCC CTGGTCCAGT TGGTTTCCCT
    GGTGACCCCG GTCCTCCTGG AGAGCCTGGT CCAGCTGGTC AAGATGGTCC CCCTGGTGAC
    AAAGGTGACG ACGGCGAACC TGGTCAGACA GGGTCCCCAG GTCCCACGGG AGAGCCAGGC
    CCCTCTGGAC CCCCAGGAAA AAGGGGCCCT CCTGGTCCAG CTGGTCCTGA AGGAAGGCAA
    GGAGAGAAAG GAGCCAAGGG TGAAGCTGGT TTGGAAGGGC CTCCTGGGAA GACAGGCCCA
    ATCGGTCCCC AGGGTGCTCC GGGGAAGCCC GGCCCTGATG GCCTCCGGGG AATTCCTGGT
    CCAGTTGGTG AACAAGGCCT TCCAGGATCC CCTGGCCCAG ATGGCCCCCC AGGCCCATTG
    GGCCCACCTG GTTTGCCTGG CCTCAAAGGA GACTCTGGTC CTAAAGGAGA AAAGGGTCAT
    CCAGGTTTAA TTGGTCTTAT TGGGCCGCCG GGTGAGCAGG GAGAAAAGGG TGACCGAGGT
    CTCCCGGGCC CCCAGGGCTC TGCAGGACCC AAGGGAGAGC AGGGTATCAC AGGTCCCTCT
    GGACCAATTG GACCCCCAGG GCCACCAGGA TTACCGGGTC CACCTGGTCC CAAAGGTGCT
    AAAGGATCAT CAGGTCCAAC GGGTCCAAAG GGTGAATCGG GTCTTCCTGG GCCTCCAGGG
    CCTCCTGGTC CTCCTGGAGA AGTCATCCAG CCTCTGCCCA TCCAGTCTTC CAAGAGGACC
    AGGCGAAACA TCGACGCCAG CCAGCTGGTG GACGATGGAA ACGCTGACAA CTACATGGAC
    TATGCAGATG GCATGGAGGA AATCTTTGGC TCACTGAATT CCTTGAAACT GGAAATTGAA
    CAAATGAAGC ATCCATTGGG CACTCAACAT AACCCAGCTC GTACCTGCAA GGATCTCCAG
    CTCTGTCACC CTGATTTCCC AGATGGTGAA TACTGGGTGG ATCCTAACCA AGGATGTTCC
    AGGGACTCTT TCAAAGTGTA CTGTAATTTC ACAGCTGGTG GAGAGACTTG CATCTTCCCT
    GATAAGAAGT CTGAAGGAGC TAGAATTACT TCTTGGCCAA AGGAAAACCC AGGCTCCTGG
    TTCAGTGAAT TCAAGCGCGG TAAACTGCTC TCCTATGTGG ATTCGGATGG GAACCCCATC
    GGAGTGGTAC AGATGACGTT CCTCAGGCTG CTGAGTGCCT CGGCCCACCA GAACATCACT
    TACAACTGCT ACCAGTCCGT AGCCTGGCAC GATGCCACCA CTGACAGCTA CGACAAGGCC
    ATCCGCTTCC TGGGCTCCAA CGACGAGGAG ATGTCCTACG ACAACAACCC CTACATCCGA
    GCTGCATTCG ATGGCTGCGC GGCAAAGAAG GGCTATCAGA AGACTGTCCT GGAAATCAAC
    ACGCCCAAGG TCGAGCAAGT GCCTATCGTC GACATCATGT TCAATGACTT CGGAGAAGCA
    TCACAGAAAT TTGGATTCGA GGTGGGACCA GCTTGCTTCA TGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa145095
    Clone ID Related Accession (Same CDS sequence) XM_015279318.2
    Accession Version XM_015279318.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5508bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product collagen alpha-1(V) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279318.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGATACGC ACACGCGATG GAAAAGGCGC AGTTGGATAC GGAACTGGCA GCTGCACGTC 
    GCGCTGGTGC TGCTGGGCGC CGCGGCCCTG GGCCGGGCAG CTGAACCTGC AGACCTCTTG
    AAGGTATTAG ATTTTCACAA TTTACCTGAT GGTATCACAA GAACAACAGG GTTTTGCACA
    AGCAGAAGAT CTTCAAAAGA AGCAGACGTT GCTTACAGAG TAACAAAAGA TGCTCAGCTC
    AGCGCCCCGA CAAAGCAGCT GTATCCTGCT TCCCCATTCC CAGAGGACTT CTCCATTTTA
    ACCACTGTAA AAGCGAAGAA AGGAGGTCAG TCCTTCTTGA TCTCAATTTA CAATGAGCAA
    GGGATCCAGC AAATTGGTGT GGAGATGGGT AGATCTCCTG TATTCCTGTA TGAAGACCAT
    ACAGGAAAGC CAGGCCCAGA AGACTATCCT CTCTTTAGAG GCATTAACCT AGCAGATGGC
    AAGTGGCACA GGGTCGCCAT CAGCGTGCAG AAGAAAAACG TCACCTTGAT TTTGGACTGT
    AAAAAGAAAA TAACCAAGTT CTTGGATCGA AGCGACCATC CCATCATTGA CGTCAATGGC
    ATCATCGTAT TTGGAACGAG GATATTAGAT GAGGAAGTCT TCGAGGGCGA CATCCAGCAG
    CTGCTGATCG TGGCCGACCC CCGGGCAGCC CACGACTACT GTGAGCACTA CAGCCCCGAC
    TGTGACACCG CCGTCCCCGA TGCTCCACAA TCCCAGGACC CCAACCAGGA TGAATACTAT
    ACCGATGGGG AGGGAGAAGG GGACACCTAC TACTATGAGT ACCCCTACTA TGAAGACGTT
    GATGAAGCTG TAAAGCCTGA AGCACCCACC ACCAAACCTG CACCCCCAGG AGTGGCTGCT
    GGAGAGAGAC CCGAAACCAA GCAGGACTAT CCTTCCCCCA CGCCATCCCC TGAAGCAGGG
    AACCCCAGCA GGCAGACCAA AGGGGCTGCA CCCGTGGACG ACCCCCTGGT GGATGAGTAC
    AACTATGAAA CCATCAACGA GGAGTACTTC ACACCTCTGC CCTATGAAGA CATCAACTAC
    AACGAAGAAG TAGACCCCCA GGGAGGGCTC ACTGAAAATG CAGTGGAGGC GGAGCTCCCC
    ACGAGTACCG TCATCACCTA CAATGAGACT GATGCTGCTC AAGGAGGAGA CGACCTGGAT
    AAAGACTTCA CCGAGGAAAC GATAAAAGAA TACGATGGGA ACTACTACTA TTATGACAGA
    ACAGTGTCTC CTGACATCGG GCCTGGGATG CCTGCCAACC AGGACACCAT CTACGAAGGG
    ATTGGAGGCC CACGGGGTGA GAAGGGGCAG AAGGGCGAGC CTGCCATTAT TGAGCCGGGT
    ATGCTTGTGG AAGGTCCCCC TGGTCCCGAA GGCCCCGCAG GCCTTCCAGG ACCTCCAGGA
    CCAACTGGAC CTGTGGGCTT AATGGGAGAT CCTGGAGAGA GAGGACCGCC TGGCCGCCCA
    GGTCTTCCAG GAGCAGATGG TTTACCAGGA CCACCAGGGA CAATGCTTAT GTTGCCTTTC
    CGCTTCAGTG GAGGAGGAGA TGCTGGCTCC AAAGGACCCA TGGTCTCTGC CCAGGAAGCT
    CAAGCCCAGG CCATCCTGCA GCAGGCGAGG CTGGCACTGA GAGGACCGGC GGGTCCAATG
    GGTCTGACCG GGCGGCCCGG CCCCATGGGA CCCCCAGGCA GTGGAGGATT GAAGGGCGAA
    GCTGGAGAAA TGGGACCTCA GGGTCCACGA GGCATCCAGG GCCCTCCCGG CCCGGCGGGG
    AAGCCAGGCC GCAGGGGACG AGCTGGCAGC GATGGTGCCC GGGGAATGCC AGGACAGACA
    GGACCAAAGG GTGACCGAGG GTTTGATGGC TTGGCTGGCT TGCCTGGCGA GAAGGGGAAC
    AGAGGTGAGC CAGGTCCCCA CGGCCCCCCG GGGGCACCCG GAGAGGACGG GGAGAGGGGG
    GATGATGGAG AAGTTGGACC CAGAGGGCTG CCCGGAGAAC CTGGCCCCCG AGGACTGCTG
    GGACCCAAAG GGCCACCAGG CCCACCGGGG CCACCGGGTG TGGCCGGTAT GGATGGACAA
    ACTGGTCCCA AGGGGAACGT GGGTCCCCAG GGTGAGCCGG GACCCCCAGG CCAGCAGGGC
    AACCCAGGTG CTCAGGGTCT TCCAGGTCCA CAAGGCCCCA TCGGTCCCCC AGGAGAGAAG
    GGGCCGCTGG GCAAACCTGG TCTTCCTGGC ATGCCTGGTG CAGATGGTCC TCCGGGTCAT
    CCTGGCAAGG AAGGTCCTCC TGGAGAGAAG GGGAGTCAGG GTCCACCAGG CCCTCAGGGT
    CCCATTGGTT ATCCAGGTCC ACGCGGAGTC AAGGGAGCGG ATGGTGTTCG TGGGTTGAAG
    GGCACCAAAG GTGAAAAGGG TGAAGATGGC TTCCCTGGCT TCAAAGGGGA TATGGGCATC
    AAGGGAGACC GGGGTGAAAT CGGACCTCCG GGCCCCAGGG GTGAGGATGG CCCTGAAGGT
    CCCAAAGGTC GCTCGGGTCC CAACGGAGAC CCCGGTCCTC TCGGTCCTGC TGGAGAGAAG
    GGAAAACTTG GCGTGCCAGG GCTGCCCGGC TACCCGGGCA GGCAGGGCCC AAAGGGCTCC
    ATTGGCTTCC CAGGATTTCC AGGCGCCAAC GGCGAGAAGG GCACACGGGG GACACCTGGC
    AAACCAGGTC CAAGGGGGCA ACGCGGTCCA ACGGGTCCAC GAGGGGAAAG AGGCCCTAGG
    GGAAGCACTG GGAAACCTGG CCCAAAGGGC AACTCTGGTG GTGATGGCCC CCCTGGTCCT
    CCTGGCGAAC GGGGACCACC AGGGCCTCAA GGACCAACCG GATTTCCTGG ACCAAAAGGC
    CCCCCTGGTC CTCCTGGGAA GGATGGATTG CCTGGTCACC CCGGACAGAG AGGAGAAACC
    GGTTTCCAAG GCAAGACTGG CCCACCTGGC CCCCCTGGTG TTGTAGGCCC TCAGGGCCCA
    ACTGGTGAAA CTGGTCCAAT GGGTGAGCGA GGACATCCAG GTCCTCCGGG TCCTCCGGGT
    GAACAAGGTC TTCCTGGCCT CACTGGGAAG GAAGGGACCA AGGGGGACCC CGGTCCTGCT
    GGCCTCCCAG GGAAGGATGG TCCCCCAGGC TTGCGAGGCT TCCCTGGAGA AAGAGGTCTG
    CCTGGCCCCA TTGGTTCTCC AGGGCTGAAA GGCAACGAAG GTCCCCCAGG CCCCCCTGGT
    CCTGCAGGCT CTCCTGGTGA GCGAGGTCCT GCGGGCTCAG CTGGCCCCAT TGGCTTACCA
    GGGCGACCTG GCCCACAGGG ACCTCCGGGA CCTGCAGGTG AAAAGGGAGC CCCGGGCGAG
    AAGGGTCCTC AAGGACCAGC AGGTCGGGAT GGCATCCAAG GCCCCGTGGG ACTCCCGGGT
    CCAGCAGGTC CTGTTGGCCC CCCCGGCGAG GATGGAGACA AGGGCGAGAT CGGGGAGCCT
    GGCCAGAAGG GCAGCAAGGG TGACAAAGGG GAGCAGGGTC CACCAGGTCC TACAGGCCCA
    CAAGGTCCGA TCGGTCAGCC AGGTCCAGCT GGTGCTGATG GAGAGCCAGG TCCCAGAGGG
    CAGCAAGGCC TCTTTGGTCA GAAAGGTGAT GAAGGACCTC GAGGTTTCCC CGGTCCCCCC
    GGCCCTGTGG GCTTGCAGGG CTTGCCTGGA CCTCCTGGTG AGAAGGGAGA AACAGGAGAT
    GTTGGGCAAA TGGGTCCACC AGGTCCACCT GGGCCCAGAG GCCCCTCCGG CCCACCAGGA
    GCAGACGGAC CCCAAGGCCC TGCTGGGGGA ATAGGAAATC CTGGTGCAGT AGGAGAGAAG
    GGTGAGCCTG GTGAATCTGG TGAGCCTGGT CTGCCTGGAG AGGTCGGCCT GCCGGGTCCA
    AAAGGTGAAA GAGGTGAAAA GGGTGAAGCA GGTCCCTCTG GTGCCGCTGG TCCACCTGGC
    CCTAAAGGTC CACCAGGTGA TGATGGTCCC AAAGGCAGCC CTGGTCCAGT TGGTTTCCCT
    GGTGACCCCG GTCCTCCTGG AGAGCCTGGT CCAGCTGGTC AAGATGGTCC CCCTGGTGAC
    AAAGGTGACG ACGGCGAACC TGGTCAGACA GGGTCCCCAG GTCCCACGGG AGAGCCAGGC
    CCCTCTGGAC CCCCAGGAAA AAGGGGCCCT CCTGGTCCAG CTGGTCCTGA AGGAAGGCAA
    GGAGAGAAAG GAGCCAAGGG TGAAGCTGGT TTGGAAGGGC CTCCTGGGAA GACAGGCCCA
    ATCGGTCCCC AGGGTGCTCC GGGGAAGCCC GGCCCTGATG GCCTCCGGGG AATTCCTGGT
    CCAGTTGGTG AACAAGGCCT TCCAGGATCC CCTGGCCCAG ATGGCCCCCC AGGCCCATTG
    GGCCCACCTG GTTTGCCTGG CCTCAAAGGA GACTCTGGTC CTAAAGGAGA AAAGGGTCAT
    CCAGGTTTAA TTGGTCTTAT TGGGCCGCCG GGTGAGCAGG GAGAAAAGGG TGACCGAGGT
    CTCCCGGGCC CCCAGGGCTC TGCAGGACCC AAGGGAGAGC AGGGTATCAC AGGTCCCTCT
    GGACCAATTG GACCCCCAGG GCCACCAGGA TTACCGGGTC CACCTGGTCC CAAAGGTGCT
    AAAGGATCAT CAGGTCCAAC GGGTCCAAAG GGTGAATCGG GTCTTCCTGG GCCTCCAGGG
    CCTCCTGGTC CTCCTGGAGA AGTCATCCAG CCTCTGCCCA TCCAGTCTTC CAAGAGGACC
    AGGCGAAACA TCGACGCCAG CCAGCTGGTG GACGATGGAA ACGCTGACAA CTACATGGAC
    TATGCAGATG GCATGGAGGA AATCTTTGGC TCACTGAATT CCTTGAAACT GGAAATTGAA
    CAAATGAAGC ATCCATTGGG CACTCAACAT AACCCAGCTC GTACCTGCAA GGATCTCCAG
    CTCTGTCACC CTGATTTCCC AGATGGTGAA TACTGGGTGG ATCCTAACCA AGGATGTTCC
    AGGGACTCTT TCAAAGTGTA CTGTAATTTC ACAGCTGGTG GAGAGACTTG CATCTTCCCT
    GATAAGAAGT CTGAAGGAAG TAAAATGGCT CGTTGGCCCA AAGAACAGCC TTCCACATGG
    TATAGTCAAT ACAAGCGGGG GTCTTTGCTC TCCTATGTGG ATTCGGATGG GAACCCCATC
    GGAGTGGTAC AGATGACGTT CCTCAGGCTG CTGAGTGCCT CGGCCCACCA GAACATCACT
    TACAACTGCT ACCAGTCCGT AGCCTGGCAC GATGCCACCA CTGACAGCTA CGACAAGGCC
    ATCCGCTTCC TGGGCTCCAA CGACGAGGAG ATGTCCTACG ACAACAACCC CTACATCCGA
    GCTGCATTCG ATGGCTGCGC GGCAAAGAAG GGCTATCAGA AGACTGTCCT GGAAATCAAC
    ACGCCCAAGG TCGAGCAAGT GCCTATCGTC GACATCATGT TCAATGACTT CGGAGAAGCA
    TCACAGAAAT TTGGATTCGA GGTGGGACCA GCTTGCTTCA TGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134804.2
    CDS259..5766
    Translation

    Target ORF information:

    RefSeq Version XM_015279318.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen type V alpha 1 chain (COL5A1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279318.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGATACGC ACACGCGATG GAAAAGGCGC AGTTGGATAC GGAACTGGCA GCTGCACGTC 
    GCGCTGGTGC TGCTGGGCGC CGCGGCCCTG GGCCGGGCAG CTGAACCTGC AGACCTCTTG
    AAGGTATTAG ATTTTCACAA TTTACCTGAT GGTATCACAA GAACAACAGG GTTTTGCACA
    AGCAGAAGAT CTTCAAAAGA AGCAGACGTT GCTTACAGAG TAACAAAAGA TGCTCAGCTC
    AGCGCCCCGA CAAAGCAGCT GTATCCTGCT TCCCCATTCC CAGAGGACTT CTCCATTTTA
    ACCACTGTAA AAGCGAAGAA AGGAGGTCAG TCCTTCTTGA TCTCAATTTA CAATGAGCAA
    GGGATCCAGC AAATTGGTGT GGAGATGGGT AGATCTCCTG TATTCCTGTA TGAAGACCAT
    ACAGGAAAGC CAGGCCCAGA AGACTATCCT CTCTTTAGAG GCATTAACCT AGCAGATGGC
    AAGTGGCACA GGGTCGCCAT CAGCGTGCAG AAGAAAAACG TCACCTTGAT TTTGGACTGT
    AAAAAGAAAA TAACCAAGTT CTTGGATCGA AGCGACCATC CCATCATTGA CGTCAATGGC
    ATCATCGTAT TTGGAACGAG GATATTAGAT GAGGAAGTCT TCGAGGGCGA CATCCAGCAG
    CTGCTGATCG TGGCCGACCC CCGGGCAGCC CACGACTACT GTGAGCACTA CAGCCCCGAC
    TGTGACACCG CCGTCCCCGA TGCTCCACAA TCCCAGGACC CCAACCAGGA TGAATACTAT
    ACCGATGGGG AGGGAGAAGG GGACACCTAC TACTATGAGT ACCCCTACTA TGAAGACGTT
    GATGAAGCTG TAAAGCCTGA AGCACCCACC ACCAAACCTG CACCCCCAGG AGTGGCTGCT
    GGAGAGAGAC CCGAAACCAA GCAGGACTAT CCTTCCCCCA CGCCATCCCC TGAAGCAGGG
    AACCCCAGCA GGCAGACCAA AGGGGCTGCA CCCGTGGACG ACCCCCTGGT GGATGAGTAC
    AACTATGAAA CCATCAACGA GGAGTACTTC ACACCTCTGC CCTATGAAGA CATCAACTAC
    AACGAAGAAG TAGACCCCCA GGGAGGGCTC ACTGAAAATG CAGTGGAGGC GGAGCTCCCC
    ACGAGTACCG TCATCACCTA CAATGAGACT GATGCTGCTC AAGGAGGAGA CGACCTGGAT
    AAAGACTTCA CCGAGGAAAC GATAAAAGAA TACGATGGGA ACTACTACTA TTATGACAGA
    ACAGTGTCTC CTGACATCGG GCCTGGGATG CCTGCCAACC AGGACACCAT CTACGAAGGG
    ATTGGAGGCC CACGGGGTGA GAAGGGGCAG AAGGGCGAGC CTGCCATTAT TGAGCCGGGT
    ATGCTTGTGG AAGGTCCCCC TGGTCCCGAA GGCCCCGCAG GCCTTCCAGG ACCTCCAGGA
    CCAACTGGAC CTGTGGGCTT AATGGGAGAT CCTGGAGAGA GAGGACCGCC TGGCCGCCCA
    GGTCTTCCAG GAGCAGATGG TTTACCAGGA CCACCAGGGA CAATGCTTAT GTTGCCTTTC
    CGCTTCAGTG GAGGAGGAGA TGCTGGCTCC AAAGGACCCA TGGTCTCTGC CCAGGAAGCT
    CAAGCCCAGG CCATCCTGCA GCAGGCGAGG CTGGCACTGA GAGGACCGGC GGGTCCAATG
    GGTCTGACCG GGCGGCCCGG CCCCATGGGA CCCCCAGGCA GTGGAGGATT GAAGGGCGAA
    GCTGGAGAAA TGGGACCTCA GGGTCCACGA GGCATCCAGG GCCCTCCCGG CCCGGCGGGG
    AAGCCAGGCC GCAGGGGACG AGCTGGCAGC GATGGTGCCC GGGGAATGCC AGGACAGACA
    GGACCAAAGG GTGACCGAGG GTTTGATGGC TTGGCTGGCT TGCCTGGCGA GAAGGGGAAC
    AGAGGTGAGC CAGGTCCCCA CGGCCCCCCG GGGGCACCCG GAGAGGACGG GGAGAGGGGG
    GATGATGGAG AAGTTGGACC CAGAGGGCTG CCCGGAGAAC CTGGCCCCCG AGGACTGCTG
    GGACCCAAAG GGCCACCAGG CCCACCGGGG CCACCGGGTG TGGCCGGTAT GGATGGACAA
    ACTGGTCCCA AGGGGAACGT GGGTCCCCAG GGTGAGCCGG GACCCCCAGG CCAGCAGGGC
    AACCCAGGTG CTCAGGGTCT TCCAGGTCCA CAAGGCCCCA TCGGTCCCCC AGGAGAGAAG
    GGGCCGCTGG GCAAACCTGG TCTTCCTGGC ATGCCTGGTG CAGATGGTCC TCCGGGTCAT
    CCTGGCAAGG AAGGTCCTCC TGGAGAGAAG GGGAGTCAGG GTCCACCAGG CCCTCAGGGT
    CCCATTGGTT ATCCAGGTCC ACGCGGAGTC AAGGGAGCGG ATGGTGTTCG TGGGTTGAAG
    GGCACCAAAG GTGAAAAGGG TGAAGATGGC TTCCCTGGCT TCAAAGGGGA TATGGGCATC
    AAGGGAGACC GGGGTGAAAT CGGACCTCCG GGCCCCAGGG GTGAGGATGG CCCTGAAGGT
    CCCAAAGGTC GCTCGGGTCC CAACGGAGAC CCCGGTCCTC TCGGTCCTGC TGGAGAGAAG
    GGAAAACTTG GCGTGCCAGG GCTGCCCGGC TACCCGGGCA GGCAGGGCCC AAAGGGCTCC
    ATTGGCTTCC CAGGATTTCC AGGCGCCAAC GGCGAGAAGG GCACACGGGG GACACCTGGC
    AAACCAGGTC CAAGGGGGCA ACGCGGTCCA ACGGGTCCAC GAGGGGAAAG AGGCCCTAGG
    GGAAGCACTG GGAAACCTGG CCCAAAGGGC AACTCTGGTG GTGATGGCCC CCCTGGTCCT
    CCTGGCGAAC GGGGACCACC AGGGCCTCAA GGACCAACCG GATTTCCTGG ACCAAAAGGC
    CCCCCTGGTC CTCCTGGGAA GGATGGATTG CCTGGTCACC CCGGACAGAG AGGAGAAACC
    GGTTTCCAAG GCAAGACTGG CCCACCTGGC CCCCCTGGTG TTGTAGGCCC TCAGGGCCCA
    ACTGGTGAAA CTGGTCCAAT GGGTGAGCGA GGACATCCAG GTCCTCCGGG TCCTCCGGGT
    GAACAAGGTC TTCCTGGCCT CACTGGGAAG GAAGGGACCA AGGGGGACCC CGGTCCTGCT
    GGCCTCCCAG GGAAGGATGG TCCCCCAGGC TTGCGAGGCT TCCCTGGAGA AAGAGGTCTG
    CCTGGCCCCA TTGGTTCTCC AGGGCTGAAA GGCAACGAAG GTCCCCCAGG CCCCCCTGGT
    CCTGCAGGCT CTCCTGGTGA GCGAGGTCCT GCGGGCTCAG CTGGCCCCAT TGGCTTACCA
    GGGCGACCTG GCCCACAGGG ACCTCCGGGA CCTGCAGGTG AAAAGGGAGC CCCGGGCGAG
    AAGGGTCCTC AAGGACCAGC AGGTCGGGAT GGCATCCAAG GCCCCGTGGG ACTCCCGGGT
    CCAGCAGGTC CTGTTGGCCC CCCCGGCGAG GATGGAGACA AGGGCGAGAT CGGGGAGCCT
    GGCCAGAAGG GCAGCAAGGG TGACAAAGGG GAGCAGGGTC CACCAGGTCC TACAGGCCCA
    CAAGGTCCGA TCGGTCAGCC AGGTCCAGCT GGTGCTGATG GAGAGCCAGG TCCCAGAGGG
    CAGCAAGGCC TCTTTGGTCA GAAAGGTGAT GAAGGACCTC GAGGTTTCCC CGGTCCCCCC
    GGCCCTGTGG GCTTGCAGGG CTTGCCTGGA CCTCCTGGTG AGAAGGGAGA AACAGGAGAT
    GTTGGGCAAA TGGGTCCACC AGGTCCACCT GGGCCCAGAG GCCCCTCCGG CCCACCAGGA
    GCAGACGGAC CCCAAGGCCC TGCTGGGGGA ATAGGAAATC CTGGTGCAGT AGGAGAGAAG
    GGTGAGCCTG GTGAATCTGG TGAGCCTGGT CTGCCTGGAG AGGTCGGCCT GCCGGGTCCA
    AAAGGTGAAA GAGGTGAAAA GGGTGAAGCA GGTCCCTCTG GTGCCGCTGG TCCACCTGGC
    CCTAAAGGTC CACCAGGTGA TGATGGTCCC AAAGGCAGCC CTGGTCCAGT TGGTTTCCCT
    GGTGACCCCG GTCCTCCTGG AGAGCCTGGT CCAGCTGGTC AAGATGGTCC CCCTGGTGAC
    AAAGGTGACG ACGGCGAACC TGGTCAGACA GGGTCCCCAG GTCCCACGGG AGAGCCAGGC
    CCCTCTGGAC CCCCAGGAAA AAGGGGCCCT CCTGGTCCAG CTGGTCCTGA AGGAAGGCAA
    GGAGAGAAAG GAGCCAAGGG TGAAGCTGGT TTGGAAGGGC CTCCTGGGAA GACAGGCCCA
    ATCGGTCCCC AGGGTGCTCC GGGGAAGCCC GGCCCTGATG GCCTCCGGGG AATTCCTGGT
    CCAGTTGGTG AACAAGGCCT TCCAGGATCC CCTGGCCCAG ATGGCCCCCC AGGCCCATTG
    GGCCCACCTG GTTTGCCTGG CCTCAAAGGA GACTCTGGTC CTAAAGGAGA AAAGGGTCAT
    CCAGGTTTAA TTGGTCTTAT TGGGCCGCCG GGTGAGCAGG GAGAAAAGGG TGACCGAGGT
    CTCCCGGGCC CCCAGGGCTC TGCAGGACCC AAGGGAGAGC AGGGTATCAC AGGTCCCTCT
    GGACCAATTG GACCCCCAGG GCCACCAGGA TTACCGGGTC CACCTGGTCC CAAAGGTGCT
    AAAGGATCAT CAGGTCCAAC GGGTCCAAAG GGTGAATCGG GTCTTCCTGG GCCTCCAGGG
    CCTCCTGGTC CTCCTGGAGA AGTCATCCAG CCTCTGCCCA TCCAGTCTTC CAAGAGGACC
    AGGCGAAACA TCGACGCCAG CCAGCTGGTG GACGATGGAA ACGCTGACAA CTACATGGAC
    TATGCAGATG GCATGGAGGA AATCTTTGGC TCACTGAATT CCTTGAAACT GGAAATTGAA
    CAAATGAAGC ATCCATTGGG CACTCAACAT AACCCAGCTC GTACCTGCAA GGATCTCCAG
    CTCTGTCACC CTGATTTCCC AGATGGTGAA TACTGGGTGG ATCCTAACCA AGGATGTTCC
    AGGGACTCTT TCAAAGTGTA CTGTAATTTC ACAGCTGGTG GAGAGACTTG CATCTTCCCT
    GATAAGAAGT CTGAAGGAAG TAAAATGGCT CGTTGGCCCA AAGAACAGCC TTCCACATGG
    TATAGTCAAT ACAAGCGGGG GTCTTTGCTC TCCTATGTGG ATTCGGATGG GAACCCCATC
    GGAGTGGTAC AGATGACGTT CCTCAGGCTG CTGAGTGCCT CGGCCCACCA GAACATCACT
    TACAACTGCT ACCAGTCCGT AGCCTGGCAC GATGCCACCA CTGACAGCTA CGACAAGGCC
    ATCCGCTTCC TGGGCTCCAA CGACGAGGAG ATGTCCTACG ACAACAACCC CTACATCCGA
    GCTGCATTCG ATGGCTGCGC GGCAAAGAAG GGCTATCAGA AGACTGTCCT GGAAATCAAC
    ACGCCCAAGG TCGAGCAAGT GCCTATCGTC GACATCATGT TCAATGACTT CGGAGAAGCA
    TCACAGAAAT TTGGATTCGA GGTGGGACCA GCTTGCTTCA TGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa28365
    Clone ID Related Accession (Same CDS sequence) NM_204790.2
    Accession Version NM_204790.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5508bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-11-05
    Organism Gallus gallus(chicken)
    Product collagen alpha-1(V) chain precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF137273.1, BU463634.1, AADN04000037.1, BM486346.1, BU114026.1, CO506693.1, BG711514.1, BM425584.1, BG710393.1, BU249831.1, U39621.1, CN218579.1 and AW355367.1. On Jul 10, 2015 this sequence version replaced NM_204790.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AF137273.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2201357, SAMEA2201358 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGATACGC ACACGCGATG GAAAAGGCGC AGTTGGATAC GGAACTGGCA GCTGCACGTC 
    GCGCTGGTGC TGCTGGGCGC CGCGGCCCTG GGCCGGGCAG CTGAACCTGC AGACCTCTTG
    AAGGTATTAG ATTTTCACAA TTTACCTGAT GGTATCACAA GAACAACAGG GTTTTGCACA
    AGCAGAAGAT CTTCAAAAGA AGCAGACGTT GCTTACAGAG TAACAAAAGA TGCTCAGCTC
    AGCGCCCCGA CAAAGCAGCT GTATCCTGCT TCCCCATTCC CAGAGGACTT CTCCATTTTA
    ACCACTGTAA AAGCGAAGAA AGGAGGTCAG TCCTTCTTGA TCTCAATTTA CAATGAGCAA
    GGGATCCAGC AAATTGGTGT GGAGATGGGT AGATCTCCTG TATTCCTGTA TGAAGACCAT
    ACAGGAAAGC CAGGCCCAGA AGACTATCCT CTCTTTAGAG GCATTAACCT AGCAGATGGC
    AAGTGGCACA GGGTCGCCAT CAGCGTGCAG AAGAAAAACG TCACCTTGAT TTTGGACTGT
    AAAAAGAAAA TAACCAAGTT CTTGGATCGA AGCGACCATC CCATCATTGA CGTCAATGGC
    ATCATCGTAT TTGGAACGAG GATATTAGAT GAGGAAGTCT TTGAGGGCGA CATCCAGCAG
    CTGCTGATCG TGGCCGACCC CCGGGCAGCC CACGACTACT GTGAGCACTA CAGCCCCGAC
    TGTGACACCG CCGTCCCCGA TGCTCCACAA TCCCAGGACC CCAACCAGGA TGAATACTAT
    ACCGATGGGG AGGGAGAAGG GGACACCTAC TACTATGAGT ACCCCTACTA TGAAGACGTT
    GATGAAGCTG TAAAGCCTGA AGCACCCACC ACCAAACCTG CACCCCCAGG AGTGGCTGCT
    GGAGAGAGAC CCGAAACCAA GCAGGACTAT CCTTCCCCCA CGCCATCCCC TGAAGCAGGG
    AACCCCAGCA GGCAGACCAA AGGGGCTGCA CCCGTGGACG ACCCCCTGGT GGATGAGTAC
    AACTATGAAA CCATCAACGA GGAGTACTTC ACACCTCTGC CCTATGAAGA CATCAACTAC
    AACGAAGAAG TAGACCCCCA GGGAGGGCTC ACTGAAAATG CAGTGGAGGC GGAGCTCCCC
    ACGAGTACCG TCATCACCTA CAATGAGACT GATGCTGCTC AAGGAGGAGA CGACCTGGAT
    AAAGACTTCA CCGAGGAAAC GATAAAAGAA TACGATGGGA ACTACTACTA TTATGACAGA
    ACAGTGTCTC CTGACATCGG GCCTGGGATG CCTGCCAACC AGGACACCAT CTACGAAGGG
    ATTGGAGGCC CACGGGGTGA GAAGGGGCAG AAGGGCGAGC CTGCCATTAT TGAGCCGGGT
    ATGCTTGTGG AAGGCCCCCC TGGTCCCGAA GGCCCCGCAG GCCTTCCAGG ACCTCCAGGA
    CCAACCGGAC CTGTGGGCTT AATGGGAGAT CCTGGAGAGA GAGGACCGCC TGGCCGCCCA
    GGTCTTCCAG GAGCAGATGG TTTACCAGGA CCACCAGGGA CAATGCTTAT GTTGCCTTTC
    CGCTTCAGTG GAGGAGGAGA TGCTGGCTCC AAAGGACCCA TGGTCTCTGC CCAGGAAGCT
    CAAGCCCAGG CCATCCTGCA GCAGGCGAGG TTGGCACTGA GAGGACCGGC GGGTCCAATG
    GGTCTGACCG GGCGGCCCGG CCCCATGGGA CCCCCAGGCA GTGGAGGATT GAAGGGCGAA
    GCTGGAGAAA TGGGACCTCA GGGTCCACGA GGCATCCAGG GCCCTCCCGG CCCGGCGGGG
    AAGCCAGGCC GCAGGGGACG AGCTGGCAGC GATGGTGCCC GGGGAATGCC AGGACAGACA
    GGACCAAAGG GTGACCGAGG GTTTGATGGC TTGGCTGGTT TGCCTGGCGA GAAGGGGAAC
    AGAGGTGAGC CAGGTCCCCA CGGCCCCCCG GGGGCACCCG GAGAGGACGG GGAGAGGGGG
    GATGATGGAG AAGTTGGACC CAGAGGGCTG CCCGGAGAAC CTGGCCCCCG AGGACTGCTG
    GGACCCAAAG GGCCACCAGG CCCACCGGGG CCACCGGGTG TGGCCGGTAT GGATGGACAA
    ACTGGTCCCA AGGGGAACGT GGGTCCCCAG GGTGAGCCGG GACCCCCAGG CCAGCAGGGC
    AACCCAGGTG CTCAGGGTCT TCCAGGTCCA CAAGGCCCCA TCGGTCCCCC AGGAGAGAAG
    GGGCCGCTGG GCAAACCTGG TCTTCCTGGC ATGCCTGGTG CAGATGGTCC TCCGGGTCAT
    CCTGGCAAGG AAGGTCCTCC TGGAGAGAAG GGGAGTCAGG GTCCACCAGG CCCTCAGGGT
    CCCATTGGTT ATCCAGGTCC ACGCGGAGTC AAGGGAGCGG ATGGTGTTCG TGGGTTGAAG
    GGCACCAAAG GTGAAAAGGG TGAAGATGGC TTCCCTGGCT TCAAAGGGGA TATGGGCATC
    AAGGGAGACC GGGGTGAAAT CGGACCTCCG GGCCCCAGGG GTGAGGATGG CCCTGAAGGT
    CCCAAAGGTC GCTCGGGTCC CAACGGAGAC CCCGGTCCTC TCGGTCCTGC TGGAGAGAAG
    GGAAAACTTG GCGTGCCAGG GCTGCCCGGC TACCCGGGCA GGCAGGGCCC AAAGGGCTCC
    ATTGGCTTCC CAGGATTTCC AGGCGCCAAC GGCGAGAAGG GCACACGGGG GACACCTGGC
    AAACCAGGTC CAAGGGGGCA ACGCGGTCCA ACGGGTCCAC GAGGGGAAAG AGGCCCTAGG
    GGAAGCACTG GGAAACCTGG CCCAAAGGGC AACTCTGGTG GTGATGGCCC CCCTGGTCCT
    CCTGGCGAAC GGGGACCACC AGGGCCTCAA GGACCAACCG GATTTCCTGG ACCAAAAGGC
    CCCCCTGGTC CTCCTGGGAA GGATGGATTG CCTGGTCACC CCGGACAGAG AGGAGAAACC
    GGTTTCCAAG GCAAGACTGG CCCACCTGGC CCCCCTGGTG TTGTAGGCCC TCAGGGCCCA
    ACTGGTGAAA CTGGTCCAAT GGGTGAGCGA GGACATCCAG GTCCTCCGGG TCCTCCGGGT
    GAACAAGGTC TTCCTGGCCT CACTGGGAAG GAAGGGACCA AGGGGGACCC CGGTCCTGCT
    GGCCTCCCAG GGAAGGATGG TCCCCCAGGC TTGCGAGGCT TCCCTGGAGA AAGAGGTCTG
    CCTGGCCCCA TTGGTTCTCC AGGGCTGAAA GGCAACGAAG GTCCCCCAGG CCCCCCTGGT
    CCTGCAGGCT CTCCTGGTGA GCGAGGTCCT GCGGGCTCAG CTGGCCCCAT TGGCTTACCA
    GGGCGACCTG GCCCACAGGG ACCTCCGGGA CCTGCAGGTG AAAAGGGAGC CCCGGGCGAG
    AAGGGTCCTC AAGGACCAGC AGGTCGGGAT GGCATCCAAG GCCCCGTGGG ACTCCCGGGT
    CCAGCAGGTC CTGTTGGCCC CCCCGGCGAG GATGGAGACA AGGGCGAGAT CGGGGAGCCT
    GGCCAGAAGG GCAGCAAGGG TGACAAAGGG GAGCAGGGTC CACCAGGTCC TACAGGCCCA
    CAAGGTCCGA TCGGTCAGCC AGGTCCAGCT GGTGCTGATG GAGAGCCGGG TCCCAGAGGG
    CAGCAAGGCC TCTTTGGTCA GAAAGGTGAT GAAGGACCTC GAGGTTTCCC CGGTCCCCCC
    GGCCCTGTGG GCTTGCAGGG CTTGCCTGGA CCTCCTGGTG AGAAGGGAGA AACAGGAGAT
    GTTGGGCAAA TGGGTCCACC AGGTCCACCT GGGCCCAGAG GCCCCTCCGG CCCACCAGGA
    GCAGACGGAC CCCAAGGCCC TGCTGGGGGA ATAGGAAATC CTGGTGCAGT AGGAGAGAAG
    GGTGAGCCTG GTGAATCTGG TGAGCCTGGT CTGCCTGGAG AGGTCGGCCT GCCGGGTCCA
    AAAGGTGAAA GAGGTGAAAA GGGTGAAGCA GGTCCCTCTG GTGCCGCTGG TCCACCTGGC
    CCTAAAGGTC CACCAGGTGA TGATGGTCCC AAAGGCAGCC CTGGTCCAGT TGGTTTCCCT
    GGTGACCCCG GTCCTCCTGG AGAGCCTGGT CCAGCTGGTC AAGATGGTCC CCCTGGTGAC
    AAAGGTGACG ACGGCGAACC TGGTCAGACA GGGTCCCCAG GTCCCACGGG AGAGCCAGGC
    CCCTCTGGAC CCCCAGGAAA AAGGGGCCCT CCTGGTCCAG CTGGTCCTGA AGGAAGGCAA
    GGAGAGAAAG GAGCCAAGGG TGAAGCTGGT TTGGAAGGGC CTCCTGGGAA GACAGGCCCA
    ATCGGTCCCC AGGGTGCTCC GGGGAAGCCC GGCCCTGATG GCCTCCGGGG AATTCCTGGT
    CCAGTTGGTG AACAAGGCCT TCCAGGATCC CCTGGCCCAG ATGGCCCCCC AGGCCCATTG
    GGCCCACCTG GTTTGCCTGG CCTCAAAGGA GACTCTGGTC CTAAAGGAGA AAAGGGTCAT
    CCAGGTTTAA TTGGTCTTAT TGGGCCGCCG GGTGAGCAGG GAGAAAAGGG TGACCGAGGT
    CTCCCGGGCC CCCAGGGCTC TGCAGGACCC AAGGGAGAGC AGGGTATCAC AGGTCCCTCT
    GGACCAATTG GACCCCCAGG GCCACCAGGA TTACCGGGTC CACCTGGTCC CAAAGGTGCT
    AAAGGATCAT CAGGTCCAAC GGGTCCAAAG GGTGAATCGG GTCTTCCTGG GCCTCCAGGG
    CCTCCTGGTC CTCCTGGAGA AGTCATCCAG CCTCTGCCCA TCCAGTCTTC CAAGAGGACC
    AGGCGAAACA TCGACGCCAG CCAGCTGGTG GACGATGGAA ACGCTGACAA CTACATGGAC
    TATGCAGATG GCATGGAGGA AATCTTCGGC TCACTGAATT CCTTGAAACT GGAAATTGAA
    CAAATGAAGC ATCCATTGGG CACTCAACAT AACCCAGCTC GTACCTGCAA GGATCTCCAG
    CTCTGTCACC CTGATTTCCC AGATGGTGAA TACTGGGTGG ATCCTAACCA AGGATGTTCC
    AGGGACTCTT TCAAAGTGTA CTGTAATTTC ACAGCTGGTG GAGAGACTTG CATCTTCCCT
    GATAAGAAGT CTGAAGGAGC TAGAATTACT TCTTGGCCAA AGGAAAACCC AGGCTCCTGG
    TTCAGTGAAT TCAAGCGCGG TAAACTGCTC TCCTATGTGG ATTCGGATGG GAACCCCATC
    GGAGTGGTAC AGATGACGTT CCTCAGGCTG CTGAGTGCCT CGGCCCACCA GAACATCACT
    TACAACTGCT ACCAGTCCGT AGCCTGGCAC GATGCCACCA CTGACAGCTA CGACAAGGCC
    ATCCGCTTCC TGGGCTCCAA CGACGAGGAG ATGTCCTACG ACAACAACCC CTACATCCGA
    GCTGCATTCG ATGGCTGCGC GGCAAAGAAG GGCTATCAGA AGACTGTCCT GGAAATCAAC
    ACGCCCAAGG TCGAGCAAGT GCCTATCGTC GACATCATGT TCAATGACTT CGGAGAAGCA
    TCACAGAAAT TTGGATTCGA GGTGGGACCA GCTTGCTTCA TGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_990121.2
    CDS1..5508
    Translation

    Target ORF information:

    RefSeq Version NM_204790.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen type V alpha 1 chain (COL5A1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_204790.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGATACGC ACACGCGATG GAAAAGGCGC AGTTGGATAC GGAACTGGCA GCTGCACGTC 
    GCGCTGGTGC TGCTGGGCGC CGCGGCCCTG GGCCGGGCAG CTGAACCTGC AGACCTCTTG
    AAGGTATTAG ATTTTCACAA TTTACCTGAT GGTATCACAA GAACAACAGG GTTTTGCACA
    AGCAGAAGAT CTTCAAAAGA AGCAGACGTT GCTTACAGAG TAACAAAAGA TGCTCAGCTC
    AGCGCCCCGA CAAAGCAGCT GTATCCTGCT TCCCCATTCC CAGAGGACTT CTCCATTTTA
    ACCACTGTAA AAGCGAAGAA AGGAGGTCAG TCCTTCTTGA TCTCAATTTA CAATGAGCAA
    GGGATCCAGC AAATTGGTGT GGAGATGGGT AGATCTCCTG TATTCCTGTA TGAAGACCAT
    ACAGGAAAGC CAGGCCCAGA AGACTATCCT CTCTTTAGAG GCATTAACCT AGCAGATGGC
    AAGTGGCACA GGGTCGCCAT CAGCGTGCAG AAGAAAAACG TCACCTTGAT TTTGGACTGT
    AAAAAGAAAA TAACCAAGTT CTTGGATCGA AGCGACCATC CCATCATTGA CGTCAATGGC
    ATCATCGTAT TTGGAACGAG GATATTAGAT GAGGAAGTCT TTGAGGGCGA CATCCAGCAG
    CTGCTGATCG TGGCCGACCC CCGGGCAGCC CACGACTACT GTGAGCACTA CAGCCCCGAC
    TGTGACACCG CCGTCCCCGA TGCTCCACAA TCCCAGGACC CCAACCAGGA TGAATACTAT
    ACCGATGGGG AGGGAGAAGG GGACACCTAC TACTATGAGT ACCCCTACTA TGAAGACGTT
    GATGAAGCTG TAAAGCCTGA AGCACCCACC ACCAAACCTG CACCCCCAGG AGTGGCTGCT
    GGAGAGAGAC CCGAAACCAA GCAGGACTAT CCTTCCCCCA CGCCATCCCC TGAAGCAGGG
    AACCCCAGCA GGCAGACCAA AGGGGCTGCA CCCGTGGACG ACCCCCTGGT GGATGAGTAC
    AACTATGAAA CCATCAACGA GGAGTACTTC ACACCTCTGC CCTATGAAGA CATCAACTAC
    AACGAAGAAG TAGACCCCCA GGGAGGGCTC ACTGAAAATG CAGTGGAGGC GGAGCTCCCC
    ACGAGTACCG TCATCACCTA CAATGAGACT GATGCTGCTC AAGGAGGAGA CGACCTGGAT
    AAAGACTTCA CCGAGGAAAC GATAAAAGAA TACGATGGGA ACTACTACTA TTATGACAGA
    ACAGTGTCTC CTGACATCGG GCCTGGGATG CCTGCCAACC AGGACACCAT CTACGAAGGG
    ATTGGAGGCC CACGGGGTGA GAAGGGGCAG AAGGGCGAGC CTGCCATTAT TGAGCCGGGT
    ATGCTTGTGG AAGGCCCCCC TGGTCCCGAA GGCCCCGCAG GCCTTCCAGG ACCTCCAGGA
    CCAACCGGAC CTGTGGGCTT AATGGGAGAT CCTGGAGAGA GAGGACCGCC TGGCCGCCCA
    GGTCTTCCAG GAGCAGATGG TTTACCAGGA CCACCAGGGA CAATGCTTAT GTTGCCTTTC
    CGCTTCAGTG GAGGAGGAGA TGCTGGCTCC AAAGGACCCA TGGTCTCTGC CCAGGAAGCT
    CAAGCCCAGG CCATCCTGCA GCAGGCGAGG TTGGCACTGA GAGGACCGGC GGGTCCAATG
    GGTCTGACCG GGCGGCCCGG CCCCATGGGA CCCCCAGGCA GTGGAGGATT GAAGGGCGAA
    GCTGGAGAAA TGGGACCTCA GGGTCCACGA GGCATCCAGG GCCCTCCCGG CCCGGCGGGG
    AAGCCAGGCC GCAGGGGACG AGCTGGCAGC GATGGTGCCC GGGGAATGCC AGGACAGACA
    GGACCAAAGG GTGACCGAGG GTTTGATGGC TTGGCTGGTT TGCCTGGCGA GAAGGGGAAC
    AGAGGTGAGC CAGGTCCCCA CGGCCCCCCG GGGGCACCCG GAGAGGACGG GGAGAGGGGG
    GATGATGGAG AAGTTGGACC CAGAGGGCTG CCCGGAGAAC CTGGCCCCCG AGGACTGCTG
    GGACCCAAAG GGCCACCAGG CCCACCGGGG CCACCGGGTG TGGCCGGTAT GGATGGACAA
    ACTGGTCCCA AGGGGAACGT GGGTCCCCAG GGTGAGCCGG GACCCCCAGG CCAGCAGGGC
    AACCCAGGTG CTCAGGGTCT TCCAGGTCCA CAAGGCCCCA TCGGTCCCCC AGGAGAGAAG
    GGGCCGCTGG GCAAACCTGG TCTTCCTGGC ATGCCTGGTG CAGATGGTCC TCCGGGTCAT
    CCTGGCAAGG AAGGTCCTCC TGGAGAGAAG GGGAGTCAGG GTCCACCAGG CCCTCAGGGT
    CCCATTGGTT ATCCAGGTCC ACGCGGAGTC AAGGGAGCGG ATGGTGTTCG TGGGTTGAAG
    GGCACCAAAG GTGAAAAGGG TGAAGATGGC TTCCCTGGCT TCAAAGGGGA TATGGGCATC
    AAGGGAGACC GGGGTGAAAT CGGACCTCCG GGCCCCAGGG GTGAGGATGG CCCTGAAGGT
    CCCAAAGGTC GCTCGGGTCC CAACGGAGAC CCCGGTCCTC TCGGTCCTGC TGGAGAGAAG
    GGAAAACTTG GCGTGCCAGG GCTGCCCGGC TACCCGGGCA GGCAGGGCCC AAAGGGCTCC
    ATTGGCTTCC CAGGATTTCC AGGCGCCAAC GGCGAGAAGG GCACACGGGG GACACCTGGC
    AAACCAGGTC CAAGGGGGCA ACGCGGTCCA ACGGGTCCAC GAGGGGAAAG AGGCCCTAGG
    GGAAGCACTG GGAAACCTGG CCCAAAGGGC AACTCTGGTG GTGATGGCCC CCCTGGTCCT
    CCTGGCGAAC GGGGACCACC AGGGCCTCAA GGACCAACCG GATTTCCTGG ACCAAAAGGC
    CCCCCTGGTC CTCCTGGGAA GGATGGATTG CCTGGTCACC CCGGACAGAG AGGAGAAACC
    GGTTTCCAAG GCAAGACTGG CCCACCTGGC CCCCCTGGTG TTGTAGGCCC TCAGGGCCCA
    ACTGGTGAAA CTGGTCCAAT GGGTGAGCGA GGACATCCAG GTCCTCCGGG TCCTCCGGGT
    GAACAAGGTC TTCCTGGCCT CACTGGGAAG GAAGGGACCA AGGGGGACCC CGGTCCTGCT
    GGCCTCCCAG GGAAGGATGG TCCCCCAGGC TTGCGAGGCT TCCCTGGAGA AAGAGGTCTG
    CCTGGCCCCA TTGGTTCTCC AGGGCTGAAA GGCAACGAAG GTCCCCCAGG CCCCCCTGGT
    CCTGCAGGCT CTCCTGGTGA GCGAGGTCCT GCGGGCTCAG CTGGCCCCAT TGGCTTACCA
    GGGCGACCTG GCCCACAGGG ACCTCCGGGA CCTGCAGGTG AAAAGGGAGC CCCGGGCGAG
    AAGGGTCCTC AAGGACCAGC AGGTCGGGAT GGCATCCAAG GCCCCGTGGG ACTCCCGGGT
    CCAGCAGGTC CTGTTGGCCC CCCCGGCGAG GATGGAGACA AGGGCGAGAT CGGGGAGCCT
    GGCCAGAAGG GCAGCAAGGG TGACAAAGGG GAGCAGGGTC CACCAGGTCC TACAGGCCCA
    CAAGGTCCGA TCGGTCAGCC AGGTCCAGCT GGTGCTGATG GAGAGCCGGG TCCCAGAGGG
    CAGCAAGGCC TCTTTGGTCA GAAAGGTGAT GAAGGACCTC GAGGTTTCCC CGGTCCCCCC
    GGCCCTGTGG GCTTGCAGGG CTTGCCTGGA CCTCCTGGTG AGAAGGGAGA AACAGGAGAT
    GTTGGGCAAA TGGGTCCACC AGGTCCACCT GGGCCCAGAG GCCCCTCCGG CCCACCAGGA
    GCAGACGGAC CCCAAGGCCC TGCTGGGGGA ATAGGAAATC CTGGTGCAGT AGGAGAGAAG
    GGTGAGCCTG GTGAATCTGG TGAGCCTGGT CTGCCTGGAG AGGTCGGCCT GCCGGGTCCA
    AAAGGTGAAA GAGGTGAAAA GGGTGAAGCA GGTCCCTCTG GTGCCGCTGG TCCACCTGGC
    CCTAAAGGTC CACCAGGTGA TGATGGTCCC AAAGGCAGCC CTGGTCCAGT TGGTTTCCCT
    GGTGACCCCG GTCCTCCTGG AGAGCCTGGT CCAGCTGGTC AAGATGGTCC CCCTGGTGAC
    AAAGGTGACG ACGGCGAACC TGGTCAGACA GGGTCCCCAG GTCCCACGGG AGAGCCAGGC
    CCCTCTGGAC CCCCAGGAAA AAGGGGCCCT CCTGGTCCAG CTGGTCCTGA AGGAAGGCAA
    GGAGAGAAAG GAGCCAAGGG TGAAGCTGGT TTGGAAGGGC CTCCTGGGAA GACAGGCCCA
    ATCGGTCCCC AGGGTGCTCC GGGGAAGCCC GGCCCTGATG GCCTCCGGGG AATTCCTGGT
    CCAGTTGGTG AACAAGGCCT TCCAGGATCC CCTGGCCCAG ATGGCCCCCC AGGCCCATTG
    GGCCCACCTG GTTTGCCTGG CCTCAAAGGA GACTCTGGTC CTAAAGGAGA AAAGGGTCAT
    CCAGGTTTAA TTGGTCTTAT TGGGCCGCCG GGTGAGCAGG GAGAAAAGGG TGACCGAGGT
    CTCCCGGGCC CCCAGGGCTC TGCAGGACCC AAGGGAGAGC AGGGTATCAC AGGTCCCTCT
    GGACCAATTG GACCCCCAGG GCCACCAGGA TTACCGGGTC CACCTGGTCC CAAAGGTGCT
    AAAGGATCAT CAGGTCCAAC GGGTCCAAAG GGTGAATCGG GTCTTCCTGG GCCTCCAGGG
    CCTCCTGGTC CTCCTGGAGA AGTCATCCAG CCTCTGCCCA TCCAGTCTTC CAAGAGGACC
    AGGCGAAACA TCGACGCCAG CCAGCTGGTG GACGATGGAA ACGCTGACAA CTACATGGAC
    TATGCAGATG GCATGGAGGA AATCTTCGGC TCACTGAATT CCTTGAAACT GGAAATTGAA
    CAAATGAAGC ATCCATTGGG CACTCAACAT AACCCAGCTC GTACCTGCAA GGATCTCCAG
    CTCTGTCACC CTGATTTCCC AGATGGTGAA TACTGGGTGG ATCCTAACCA AGGATGTTCC
    AGGGACTCTT TCAAAGTGTA CTGTAATTTC ACAGCTGGTG GAGAGACTTG CATCTTCCCT
    GATAAGAAGT CTGAAGGAGC TAGAATTACT TCTTGGCCAA AGGAAAACCC AGGCTCCTGG
    TTCAGTGAAT TCAAGCGCGG TAAACTGCTC TCCTATGTGG ATTCGGATGG GAACCCCATC
    GGAGTGGTAC AGATGACGTT CCTCAGGCTG CTGAGTGCCT CGGCCCACCA GAACATCACT
    TACAACTGCT ACCAGTCCGT AGCCTGGCAC GATGCCACCA CTGACAGCTA CGACAAGGCC
    ATCCGCTTCC TGGGCTCCAA CGACGAGGAG ATGTCCTACG ACAACAACCC CTACATCCGA
    GCTGCATTCG ATGGCTGCGC GGCAAAGAAG GGCTATCAGA AGACTGTCCT GGAAATCAAC
    ACGCCCAAGG TCGAGCAAGT GCCTATCGTC GACATCATGT TCAATGACTT CGGAGAAGCA
    TCACAGAAAT TTGGATTCGA GGTGGGACCA GCTTGCTTCA TGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43341
    Clone ID Related Accession (Same CDS sequence) XM_015279318.1
    Accession Version XM_015279318.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5508bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product collagen alpha-1(V) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGATACGC ACACGCGATG GAAAAGGCGC AGTTGGATAC GGAACTGGCA GCTGCACGTC 
    GCGCTGGTGC TGCTGGGCGC CGCGGCCCTG GGCCGGGCAG CTGAACCTGC AGACCTCTTG
    AAGGTATTAG ATTTTCACAA TTTACCTGAT GGTATCACAA GAACAACAGG GTTTTGCACA
    AGCAGAAGAT CTTCAAAAGA AGCAGACGTT GCTTACAGAG TAACAAAAGA TGCTCAGCTC
    AGCGCCCCGA CAAAGCAGCT GTATCCTGCT TCCCCATTCC CAGAGGACTT CTCCATTTTA
    ACCACTGTAA AAGCGAAGAA AGGAGGTCAG TCCTTCTTGA TCTCAATTTA CAATGAGCAA
    GGGATCCAGC AAATTGGTGT GGAGATGGGT AGATCTCCTG TATTCCTGTA TGAAGACCAT
    ACAGGAAAGC CAGGCCCAGA AGACTATCCT CTCTTTAGAG GCATTAACCT AGCAGATGGC
    AAGTGGCACA GGGTCGCCAT CAGCGTGCAG AAGAAAAACG TCACCTTGAT TTTGGACTGT
    AAAAAGAAAA TAACCAAGTT CTTGGATCGA AGCGACCATC CCATCATTGA CGTCAATGGC
    ATCATCGTAT TTGGAACGAG GATATTAGAT GAGGAAGTCT TCGAGGGCGA CATCCAGCAG
    CTGCTGATCG TGGCCGACCC CCGGGCAGCC CACGACTACT GTGAGCACTA CAGCCCCGAC
    TGTGACACCG CCGTCCCCGA TGCTCCACAA TCCCAGGACC CCAACCAGGA TGAATACTAT
    ACCGATGGGG AGGGAGAAGG GGACACCTAC TACTATGAGT ACCCCTACTA TGAAGACGTT
    GATGAAGCTG TAAAGCCTGA AGCACCCACC ACCAAACCTG CACCCCCAGG AGTGGCTGCT
    GGAGAGAGAC CCGAAACCAA GCAGGACTAT CCTTCCCCCA CGCCATCCCC TGAAGCAGGG
    AACCCCAGCA GGCAGACCAA AGGGGCTGCA CCCGTGGACG ACCCCCTGGT GGATGAGTAC
    AACTATGAAA CCATCAACGA GGAGTACTTC ACACCTCTGC CCTATGAAGA CATCAACTAC
    AACGAAGAAG TAGACCCCCA GGGAGGGCTC ACTGAAAATG CAGTGGAGGC GGAGCTCCCC
    ACGAGTACCG TCATCACCTA CAATGAGACT GATGCTGCTC AAGGAGGAGA CGACCTGGAT
    AAAGACTTCA CCGAGGAAAC GATAAAAGAA TACGATGGGA ACTACTACTA TTATGACAGA
    ACAGTGTCTC CTGACATCGG GCCTGGGATG CCTGCCAACC AGGACACCAT CTACGAAGGG
    ATTGGAGGCC CACGGGGTGA GAAGGGGCAG AAGGGCGAGC CTGCCATTAT TGAGCCGGGT
    ATGCTTGTGG AAGGTCCCCC TGGTCCCGAA GGCCCCGCAG GCCTTCCAGG ACCTCCAGGA
    CCAACTGGAC CTGTGGGCTT AATGGGAGAT CCTGGAGAGA GAGGACCGCC TGGCCGCCCA
    GGTCTTCCAG GAGCAGATGG TTTACCAGGA CCACCAGGGA CAATGCTTAT GTTGCCTTTC
    CGCTTCAGTG GAGGAGGAGA TGCTGGCTCC AAAGGACCCA TGGTCTCTGC CCAGGAAGCT
    CAAGCCCAGG CCATCCTGCA GCAGGCGAGG CTGGCACTGA GAGGACCGGC GGGTCCAATG
    GGTCTGACCG GGCGGCCCGG CCCCATGGGA CCCCCAGGCA GTGGAGGATT GAAGGGCGAA
    GCTGGAGAAA TGGGACCTCA GGGTCCACGA GGCATCCAGG GCCCTCCCGG CCCGGCGGGG
    AAGCCAGGCC GCAGGGGACG AGCTGGCAGC GATGGTGCCC GGGGAATGCC AGGACAGACA
    GGACCAAAGG GTGACCGAGG GTTTGATGGC TTGGCTGGCT TGCCTGGCGA GAAGGGGAAC
    AGAGGTGAGC CAGGTCCCCA CGGCCCCCCG GGGGCACCCG GAGAGGACGG GGAGAGGGGG
    GATGATGGAG AAGTTGGACC CAGAGGGCTG CCCGGAGAAC CTGGCCCCCG AGGACTGCTG
    GGACCCAAAG GGCCACCAGG CCCACCGGGG CCACCGGGTG TGGCCGGTAT GGATGGACAA
    ACTGGTCCCA AGGGGAACGT GGGTCCCCAG GGTGAGCCGG GACCCCCAGG CCAGCAGGGC
    AACCCAGGTG CTCAGGGTCT TCCAGGTCCA CAAGGCCCCA TCGGTCCCCC AGGAGAGAAG
    GGGCCGCTGG GCAAACCTGG TCTTCCTGGC ATGCCTGGTG CAGATGGTCC TCCGGGTCAT
    CCTGGCAAGG AAGGTCCTCC TGGAGAGAAG GGGAGTCAGG GTCCACCAGG CCCTCAGGGT
    CCCATTGGTT ATCCAGGTCC ACGCGGAGTC AAGGGAGCGG ATGGTGTTCG TGGGTTGAAG
    GGCACCAAAG GTGAAAAGGG TGAAGATGGC TTCCCTGGCT TCAAAGGGGA TATGGGCATC
    AAGGGAGACC GGGGTGAAAT CGGACCTCCG GGCCCCAGGG GTGAGGATGG CCCTGAAGGT
    CCCAAAGGTC GCTCGGGTCC CAACGGAGAC CCCGGTCCTC TCGGTCCTGC TGGAGAGAAG
    GGAAAACTTG GCGTGCCAGG GCTGCCCGGC TACCCGGGCA GGCAGGGCCC AAAGGGCTCC
    ATTGGCTTCC CAGGATTTCC AGGCGCCAAC GGCGAGAAGG GCACACGGGG GACACCTGGC
    AAACCAGGTC CAAGGGGGCA ACGCGGTCCA ACGGGTCCAC GAGGGGAAAG AGGCCCTAGG
    GGAAGCACTG GGAAACCTGG CCCAAAGGGC AACTCTGGTG GTGATGGCCC CCCTGGTCCT
    CCTGGCGAAC GGGGACCACC AGGGCCTCAA GGACCAACCG GATTTCCTGG ACCAAAAGGC
    CCCCCTGGTC CTCCTGGGAA GGATGGATTG CCTGGTCACC CCGGACAGAG AGGAGAAACC
    GGTTTCCAAG GCAAGACTGG CCCACCTGGC CCCCCTGGTG TTGTAGGCCC TCAGGGCCCA
    ACTGGTGAAA CTGGTCCAAT GGGTGAGCGA GGACATCCAG GTCCTCCGGG TCCTCCGGGT
    GAACAAGGTC TTCCTGGCCT CACTGGGAAG GAAGGGACCA AGGGGGACCC CGGTCCTGCT
    GGCCTCCCAG GGAAGGATGG TCCCCCAGGC TTGCGAGGCT TCCCTGGAGA AAGAGGTCTG
    CCTGGCCCCA TTGGTTCTCC AGGGCTGAAA GGCAACGAAG GTCCCCCAGG CCCCCCTGGT
    CCTGCAGGCT CTCCTGGTGA GCGAGGTCCT GCGGGCTCAG CTGGCCCCAT TGGCTTACCA
    GGGCGACCTG GCCCACAGGG ACCTCCGGGA CCTGCAGGTG AAAAGGGAGC CCCGGGCGAG
    AAGGGTCCTC AAGGACCAGC AGGTCGGGAT GGCATCCAAG GCCCCGTGGG ACTCCCGGGT
    CCAGCAGGTC CTGTTGGCCC CCCCGGCGAG GATGGAGACA AGGGCGAGAT CGGGGAGCCT
    GGCCAGAAGG GCAGCAAGGG TGACAAAGGG GAGCAGGGTC CACCAGGTCC TACAGGCCCA
    CAAGGTCCGA TCGGTCAGCC AGGTCCAGCT GGTGCTGATG GAGAGCCAGG TCCCAGAGGG
    CAGCAAGGCC TCTTTGGTCA GAAAGGTGAT GAAGGTCCTC GAGGTTTCCC CGGTCCCCCC
    GGCCCTGTGG GCTTGCAGGG CTTGCCTGGA CCTCCTGGTG AGAAGGGAGA AACAGGAGAT
    GTTGGGCAAA TGGGTCCACC AGGTCCACCT GGGCCCAGAG GCCCCTCCGG CCCACCAGGA
    GCAGACGGAC CCCAAGGCCC TGCTGGGGGA ATAGGAAATC CTGGTGCAGT AGGAGAGAAG
    GGTGAGCCTG GTGAATCTGG TGAGCCTGGT CTGCCTGGAG AGGTCGGCCT GCCGGGTCCA
    AAAGGTGAAA GAGGTGAAAA GGGTGAAGCA GGTCCCTCTG GAGCAGCTGG ACCACCTGGT
    GCTAAAGGTC CACCAGGTGA TGATGGTCCC AAAGGCAGCC CTGGTCCAGT TGGTTTCCCT
    GGTGACCCCG GTCCTCCTGG AGAGCCTGGT CCAGCTGGTC AAGATGGTCC CCCTGGTGAC
    AAAGGTGACG ACGGCGAACC TGGTCAGACA GGGTCCCCAG GTCCCACGGG AGAGCCAGGC
    CCCTCTGGAC CCCCAGGAAA AAGGGGCCCT CCTGGTCCAG CTGGTCCTGA AGGAAGGCAA
    GGAGAGAAAG GAGCCAAGGG TGAAGCTGGT TTGGAAGGGC CTCCTGGGAA GACAGGCCCA
    ATCGGTCCCC AGGGTGCTCC GGGGAAGCCC GGCCCTGATG GCCTCCGGGG AATTCCTGGT
    CCAGTTGGTG AACAAGGCCT TCCAGGATCC CCTGGCCCAG ATGGCCCCCC AGGCCCATTG
    GGCCCACCTG GTTTGCCTGG CCTCAAAGGA GACTCTGGTC CTAAAGGAGA AAAGGGTCAT
    CCAGGTTTAA TTGGTCTTAT TGGGCCGCCG GGTGAGCAGG GAGAAAAGGG TGACCGAGGT
    CTCCCGGGCC CCCAGGGCTC TGCAGGACCC AAGGGAGAGC AGGGTATCAC AGGTCCCTCT
    GGACCAATTG GACCCCCAGG GCCACCAGGA TTACCGGGTC CACCTGGTCC CAAAGGTGCT
    AAAGGATCAT CAGGTCCAAC GGGTCCAAAG GGTGAATCGG GTCTTCCTGG GCCTCCAGGG
    CCTCCTGGTC CTCCTGGAGA AGTCATCCAG CCTCTGCCCA TCCAGTCTTC CAAGAGGACC
    AGGCGAAACA TCGACGCCAG CCAGCTGGTG GACGATGGAA ACGCTGACAA CTACATGGAC
    TATGCAGATG GCATGGAGGA AATCTTTGGC TCACTGAATT CCTTGAAACT GGAAATTGAA
    CAAATGAAGC ATCCATTGGG CACTCAACAT AACCCAGCTC GTACCTGCAA GGATCTCCAG
    CTCTGTCACC CTGATTTCCC AGATGGTGAA TACTGGGTGG ATCCTAACCA AGGATGTTCC
    AGGGACTCTT TCAAAGTGTA CTGTAATTTC ACAGCTGGTG GAGAGACTTG CATCTTCCCT
    GATAAGAAGT CTGAAGGAAG TAAAATGGCT CGTTGGCCCA AAGAACAGCC TTCCACATGG
    TATAGTCAAT ACAAGCGGGG GTCTTTGCTC TCCTATGTGG ATTCGGATGG GAACCCCATC
    GGAGTGGTAC AGATGACGTT CCTCAGGCTG CTGAGTGCCT CGGCCCACCA GAACATCACT
    TACAACTGCT ACCAGTCCGT AGCCTGGCAC GATGCCACCA CTGACAGCTA CGACAAGGCC
    ATCCGCTTCC TGGGCTCCAA CGACGAGGAG ATGTCCTACG ACAACAACCC CTACATCCGA
    GCTGCATTCG ATGGCTGCGC GGCAAAGAAG GGCTATCAGA AGACTGTCCT GGAAATCAAC
    ACGCCCAAGG TCGAGCAAGT GCCTATCGTC GACATCATGT TCAATGACTT CGGAGAAGCA
    TCACAGAAAT TTGGATTCGA GGTGGGACCA GCTTGCTTCA TGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134804.1
    CDS266..5773
    Translation

    Target ORF information:

    RefSeq Version XM_015279318.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen, type V, alpha 1 (COL5A1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279318.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGATACGC ACACGCGATG GAAAAGGCGC AGTTGGATAC GGAACTGGCA GCTGCACGTC 
    GCGCTGGTGC TGCTGGGCGC CGCGGCCCTG GGCCGGGCAG CTGAACCTGC AGACCTCTTG
    AAGGTATTAG ATTTTCACAA TTTACCTGAT GGTATCACAA GAACAACAGG GTTTTGCACA
    AGCAGAAGAT CTTCAAAAGA AGCAGACGTT GCTTACAGAG TAACAAAAGA TGCTCAGCTC
    AGCGCCCCGA CAAAGCAGCT GTATCCTGCT TCCCCATTCC CAGAGGACTT CTCCATTTTA
    ACCACTGTAA AAGCGAAGAA AGGAGGTCAG TCCTTCTTGA TCTCAATTTA CAATGAGCAA
    GGGATCCAGC AAATTGGTGT GGAGATGGGT AGATCTCCTG TATTCCTGTA TGAAGACCAT
    ACAGGAAAGC CAGGCCCAGA AGACTATCCT CTCTTTAGAG GCATTAACCT AGCAGATGGC
    AAGTGGCACA GGGTCGCCAT CAGCGTGCAG AAGAAAAACG TCACCTTGAT TTTGGACTGT
    AAAAAGAAAA TAACCAAGTT CTTGGATCGA AGCGACCATC CCATCATTGA CGTCAATGGC
    ATCATCGTAT TTGGAACGAG GATATTAGAT GAGGAAGTCT TCGAGGGCGA CATCCAGCAG
    CTGCTGATCG TGGCCGACCC CCGGGCAGCC CACGACTACT GTGAGCACTA CAGCCCCGAC
    TGTGACACCG CCGTCCCCGA TGCTCCACAA TCCCAGGACC CCAACCAGGA TGAATACTAT
    ACCGATGGGG AGGGAGAAGG GGACACCTAC TACTATGAGT ACCCCTACTA TGAAGACGTT
    GATGAAGCTG TAAAGCCTGA AGCACCCACC ACCAAACCTG CACCCCCAGG AGTGGCTGCT
    GGAGAGAGAC CCGAAACCAA GCAGGACTAT CCTTCCCCCA CGCCATCCCC TGAAGCAGGG
    AACCCCAGCA GGCAGACCAA AGGGGCTGCA CCCGTGGACG ACCCCCTGGT GGATGAGTAC
    AACTATGAAA CCATCAACGA GGAGTACTTC ACACCTCTGC CCTATGAAGA CATCAACTAC
    AACGAAGAAG TAGACCCCCA GGGAGGGCTC ACTGAAAATG CAGTGGAGGC GGAGCTCCCC
    ACGAGTACCG TCATCACCTA CAATGAGACT GATGCTGCTC AAGGAGGAGA CGACCTGGAT
    AAAGACTTCA CCGAGGAAAC GATAAAAGAA TACGATGGGA ACTACTACTA TTATGACAGA
    ACAGTGTCTC CTGACATCGG GCCTGGGATG CCTGCCAACC AGGACACCAT CTACGAAGGG
    ATTGGAGGCC CACGGGGTGA GAAGGGGCAG AAGGGCGAGC CTGCCATTAT TGAGCCGGGT
    ATGCTTGTGG AAGGTCCCCC TGGTCCCGAA GGCCCCGCAG GCCTTCCAGG ACCTCCAGGA
    CCAACTGGAC CTGTGGGCTT AATGGGAGAT CCTGGAGAGA GAGGACCGCC TGGCCGCCCA
    GGTCTTCCAG GAGCAGATGG TTTACCAGGA CCACCAGGGA CAATGCTTAT GTTGCCTTTC
    CGCTTCAGTG GAGGAGGAGA TGCTGGCTCC AAAGGACCCA TGGTCTCTGC CCAGGAAGCT
    CAAGCCCAGG CCATCCTGCA GCAGGCGAGG CTGGCACTGA GAGGACCGGC GGGTCCAATG
    GGTCTGACCG GGCGGCCCGG CCCCATGGGA CCCCCAGGCA GTGGAGGATT GAAGGGCGAA
    GCTGGAGAAA TGGGACCTCA GGGTCCACGA GGCATCCAGG GCCCTCCCGG CCCGGCGGGG
    AAGCCAGGCC GCAGGGGACG AGCTGGCAGC GATGGTGCCC GGGGAATGCC AGGACAGACA
    GGACCAAAGG GTGACCGAGG GTTTGATGGC TTGGCTGGCT TGCCTGGCGA GAAGGGGAAC
    AGAGGTGAGC CAGGTCCCCA CGGCCCCCCG GGGGCACCCG GAGAGGACGG GGAGAGGGGG
    GATGATGGAG AAGTTGGACC CAGAGGGCTG CCCGGAGAAC CTGGCCCCCG AGGACTGCTG
    GGACCCAAAG GGCCACCAGG CCCACCGGGG CCACCGGGTG TGGCCGGTAT GGATGGACAA
    ACTGGTCCCA AGGGGAACGT GGGTCCCCAG GGTGAGCCGG GACCCCCAGG CCAGCAGGGC
    AACCCAGGTG CTCAGGGTCT TCCAGGTCCA CAAGGCCCCA TCGGTCCCCC AGGAGAGAAG
    GGGCCGCTGG GCAAACCTGG TCTTCCTGGC ATGCCTGGTG CAGATGGTCC TCCGGGTCAT
    CCTGGCAAGG AAGGTCCTCC TGGAGAGAAG GGGAGTCAGG GTCCACCAGG CCCTCAGGGT
    CCCATTGGTT ATCCAGGTCC ACGCGGAGTC AAGGGAGCGG ATGGTGTTCG TGGGTTGAAG
    GGCACCAAAG GTGAAAAGGG TGAAGATGGC TTCCCTGGCT TCAAAGGGGA TATGGGCATC
    AAGGGAGACC GGGGTGAAAT CGGACCTCCG GGCCCCAGGG GTGAGGATGG CCCTGAAGGT
    CCCAAAGGTC GCTCGGGTCC CAACGGAGAC CCCGGTCCTC TCGGTCCTGC TGGAGAGAAG
    GGAAAACTTG GCGTGCCAGG GCTGCCCGGC TACCCGGGCA GGCAGGGCCC AAAGGGCTCC
    ATTGGCTTCC CAGGATTTCC AGGCGCCAAC GGCGAGAAGG GCACACGGGG GACACCTGGC
    AAACCAGGTC CAAGGGGGCA ACGCGGTCCA ACGGGTCCAC GAGGGGAAAG AGGCCCTAGG
    GGAAGCACTG GGAAACCTGG CCCAAAGGGC AACTCTGGTG GTGATGGCCC CCCTGGTCCT
    CCTGGCGAAC GGGGACCACC AGGGCCTCAA GGACCAACCG GATTTCCTGG ACCAAAAGGC
    CCCCCTGGTC CTCCTGGGAA GGATGGATTG CCTGGTCACC CCGGACAGAG AGGAGAAACC
    GGTTTCCAAG GCAAGACTGG CCCACCTGGC CCCCCTGGTG TTGTAGGCCC TCAGGGCCCA
    ACTGGTGAAA CTGGTCCAAT GGGTGAGCGA GGACATCCAG GTCCTCCGGG TCCTCCGGGT
    GAACAAGGTC TTCCTGGCCT CACTGGGAAG GAAGGGACCA AGGGGGACCC CGGTCCTGCT
    GGCCTCCCAG GGAAGGATGG TCCCCCAGGC TTGCGAGGCT TCCCTGGAGA AAGAGGTCTG
    CCTGGCCCCA TTGGTTCTCC AGGGCTGAAA GGCAACGAAG GTCCCCCAGG CCCCCCTGGT
    CCTGCAGGCT CTCCTGGTGA GCGAGGTCCT GCGGGCTCAG CTGGCCCCAT TGGCTTACCA
    GGGCGACCTG GCCCACAGGG ACCTCCGGGA CCTGCAGGTG AAAAGGGAGC CCCGGGCGAG
    AAGGGTCCTC AAGGACCAGC AGGTCGGGAT GGCATCCAAG GCCCCGTGGG ACTCCCGGGT
    CCAGCAGGTC CTGTTGGCCC CCCCGGCGAG GATGGAGACA AGGGCGAGAT CGGGGAGCCT
    GGCCAGAAGG GCAGCAAGGG TGACAAAGGG GAGCAGGGTC CACCAGGTCC TACAGGCCCA
    CAAGGTCCGA TCGGTCAGCC AGGTCCAGCT GGTGCTGATG GAGAGCCAGG TCCCAGAGGG
    CAGCAAGGCC TCTTTGGTCA GAAAGGTGAT GAAGGTCCTC GAGGTTTCCC CGGTCCCCCC
    GGCCCTGTGG GCTTGCAGGG CTTGCCTGGA CCTCCTGGTG AGAAGGGAGA AACAGGAGAT
    GTTGGGCAAA TGGGTCCACC AGGTCCACCT GGGCCCAGAG GCCCCTCCGG CCCACCAGGA
    GCAGACGGAC CCCAAGGCCC TGCTGGGGGA ATAGGAAATC CTGGTGCAGT AGGAGAGAAG
    GGTGAGCCTG GTGAATCTGG TGAGCCTGGT CTGCCTGGAG AGGTCGGCCT GCCGGGTCCA
    AAAGGTGAAA GAGGTGAAAA GGGTGAAGCA GGTCCCTCTG GAGCAGCTGG ACCACCTGGT
    GCTAAAGGTC CACCAGGTGA TGATGGTCCC AAAGGCAGCC CTGGTCCAGT TGGTTTCCCT
    GGTGACCCCG GTCCTCCTGG AGAGCCTGGT CCAGCTGGTC AAGATGGTCC CCCTGGTGAC
    AAAGGTGACG ACGGCGAACC TGGTCAGACA GGGTCCCCAG GTCCCACGGG AGAGCCAGGC
    CCCTCTGGAC CCCCAGGAAA AAGGGGCCCT CCTGGTCCAG CTGGTCCTGA AGGAAGGCAA
    GGAGAGAAAG GAGCCAAGGG TGAAGCTGGT TTGGAAGGGC CTCCTGGGAA GACAGGCCCA
    ATCGGTCCCC AGGGTGCTCC GGGGAAGCCC GGCCCTGATG GCCTCCGGGG AATTCCTGGT
    CCAGTTGGTG AACAAGGCCT TCCAGGATCC CCTGGCCCAG ATGGCCCCCC AGGCCCATTG
    GGCCCACCTG GTTTGCCTGG CCTCAAAGGA GACTCTGGTC CTAAAGGAGA AAAGGGTCAT
    CCAGGTTTAA TTGGTCTTAT TGGGCCGCCG GGTGAGCAGG GAGAAAAGGG TGACCGAGGT
    CTCCCGGGCC CCCAGGGCTC TGCAGGACCC AAGGGAGAGC AGGGTATCAC AGGTCCCTCT
    GGACCAATTG GACCCCCAGG GCCACCAGGA TTACCGGGTC CACCTGGTCC CAAAGGTGCT
    AAAGGATCAT CAGGTCCAAC GGGTCCAAAG GGTGAATCGG GTCTTCCTGG GCCTCCAGGG
    CCTCCTGGTC CTCCTGGAGA AGTCATCCAG CCTCTGCCCA TCCAGTCTTC CAAGAGGACC
    AGGCGAAACA TCGACGCCAG CCAGCTGGTG GACGATGGAA ACGCTGACAA CTACATGGAC
    TATGCAGATG GCATGGAGGA AATCTTTGGC TCACTGAATT CCTTGAAACT GGAAATTGAA
    CAAATGAAGC ATCCATTGGG CACTCAACAT AACCCAGCTC GTACCTGCAA GGATCTCCAG
    CTCTGTCACC CTGATTTCCC AGATGGTGAA TACTGGGTGG ATCCTAACCA AGGATGTTCC
    AGGGACTCTT TCAAAGTGTA CTGTAATTTC ACAGCTGGTG GAGAGACTTG CATCTTCCCT
    GATAAGAAGT CTGAAGGAAG TAAAATGGCT CGTTGGCCCA AAGAACAGCC TTCCACATGG
    TATAGTCAAT ACAAGCGGGG GTCTTTGCTC TCCTATGTGG ATTCGGATGG GAACCCCATC
    GGAGTGGTAC AGATGACGTT CCTCAGGCTG CTGAGTGCCT CGGCCCACCA GAACATCACT
    TACAACTGCT ACCAGTCCGT AGCCTGGCAC GATGCCACCA CTGACAGCTA CGACAAGGCC
    ATCCGCTTCC TGGGCTCCAA CGACGAGGAG ATGTCCTACG ACAACAACCC CTACATCCGA
    GCTGCATTCG ATGGCTGCGC GGCAAAGAAG GGCTATCAGA AGACTGTCCT GGAAATCAAC
    ACGCCCAAGG TCGAGCAAGT GCCTATCGTC GACATCATGT TCAATGACTT CGGAGAAGCA
    TCACAGAAAT TTGGATTCGA GGTGGGACCA GCTTGCTTCA TGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.