gRNAs
The following COL5A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL5A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OGa153315 | |
| Clone ID Related Accession (Same CDS sequence) | NM_204790.3 | |
| Accession Version | NM_204790.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 5508bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-01-11 | |
| Organism | Gallus gallus(chicken) | |
| Product | collagen alpha-1(V) chain precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000150.1. On Dec 20, 2018 this sequence version replaced NM_204790.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AF137273.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2201357, SAMEA2201358 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGATACGC ACACGCGATG GAAAAGGCGC AGTTGGATAC GGAACTGGCA GCTGCACGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_990121.2 |
| CDS | 1..5508 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_204790.3 |
1 | ATGGATACGC ACACGCGATG GAAAAGGCGC AGTTGGATAC GGAACTGGCA GCTGCACGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa145095 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015279318.2 | |
| Accession Version | XM_015279318.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 5508bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-16 | |
| Organism | Gallus gallus(chicken) | |
| Product | collagen alpha-1(V) chain isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279318.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGATACGC ACACGCGATG GAAAAGGCGC AGTTGGATAC GGAACTGGCA GCTGCACGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015134804.2 |
| CDS | 259..5766 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015279318.2 |
1 | ATGGATACGC ACACGCGATG GAAAAGGCGC AGTTGGATAC GGAACTGGCA GCTGCACGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa28365 | |
| Clone ID Related Accession (Same CDS sequence) | NM_204790.2 | |
| Accession Version | NM_204790.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 5508bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-11-05 | |
| Organism | Gallus gallus(chicken) | |
| Product | collagen alpha-1(V) chain precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF137273.1, BU463634.1, AADN04000037.1, BM486346.1, BU114026.1, CO506693.1, BG711514.1, BM425584.1, BG710393.1, BU249831.1, U39621.1, CN218579.1 and AW355367.1. On Jul 10, 2015 this sequence version replaced NM_204790.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AF137273.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2201357, SAMEA2201358 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGATACGC ACACGCGATG GAAAAGGCGC AGTTGGATAC GGAACTGGCA GCTGCACGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_990121.2 |
| CDS | 1..5508 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_204790.2 |
1 | ATGGATACGC ACACGCGATG GAAAAGGCGC AGTTGGATAC GGAACTGGCA GCTGCACGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa43341 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015279318.1 | |
| Accession Version | XM_015279318.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 5508bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-01-03 | |
| Organism | Gallus gallus(chicken) | |
| Product | collagen alpha-1(V) chain isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGATACGC ACACGCGATG GAAAAGGCGC AGTTGGATAC GGAACTGGCA GCTGCACGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015134804.1 |
| CDS | 266..5773 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015279318.1 |
1 | ATGGATACGC ACACGCGATG GAAAAGGCGC AGTTGGATAC GGAACTGGCA GCTGCACGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Chicken genomics resource: sequencing and annotation of 35,407 ESTs from single and multiple tissue cDNA libraries and CAP3 assembly of a chicken gene index. |
ESTs from brain and testis of White Leghorn and red junglefowl: annotation, bioinformatic classification of unknown transcripts and analysis of expression levels. |
A comprehensive collection of chicken cDNAs. |
Complete primary structure of the chicken alpha1(V) collagen chain. |
Reduction of type V collagen using a dominant-negative strategy alters the regulation of fibrillogenesis and results in the loss of corneal-specific fibril morphology. |
