COL4A1 cDNA ORF clone, Gallus gallus(chicken)

The following COL4A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL4A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa142049 XM_025156021.1
Latest version!
Gallus gallus collagen type IV alpha 1 chain (COL4A1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa24130 NM_001162399.3
Latest version!
Gallus gallus collagen type IV alpha 1 chain (COL4A1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OGa30657 XM_015277823.2
Latest version!
Gallus gallus collagen type IV alpha 1 chain (COL4A1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OGa30658 XM_015277824.2
Latest version!
Gallus gallus collagen type IV alpha 1 chain (COL4A1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
View Old Accession Versions >>
OGa30657 XM_015277823.1 Gallus gallus collagen, type IV, alpha 1 (COL4A1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OGa24130 NM_001162399.2 Gallus gallus collagen type IV alpha 1 chain (COL4A1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OGa30658 XM_015277824.1 Gallus gallus collagen, type IV, alpha 1 (COL4A1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa142049
    Clone ID Related Accession (Same CDS sequence) XM_025156021.1
    Accession Version XM_025156021.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product collagen alpha-1(IV) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCAAGGAC CTGAAGGTCC CCCTGGGCCA CCAGGACTTA AGGGTGATAC TGGAGAACCA 
    GGGCTGCCAG GAACAAAAGG AACAAGAGGC CCACCAGGAC CATCAGGATT TCCAGGAAAC
    CCAGGCCTCC CTGGCATTCC TGGACAAGAT GGCCCTCCTG GTCCACCTGG CATTCCAGGA
    TGTAATGGCA CAAAGGGTGA AAGAGGTCCA GTAGGTCCCC CAGGTTTACC GGGACTGACT
    GGAAGCATAG GACCCCCAGG ACCTCCTGGA ATTAAGGGAG ATCCAGGTGA AGTGATTGGT
    ATTTTACCTC CTAGTGTGCT AAAAGGTGAT AAAGGCTTTC CTGGCCAACC TGGACTACCT
    GGTCCACCTG GAACTCCAGG GTTTCAGGGA CCAATGGGGC CACCAGGGAT TCCAGGAGAA
    CCAGGTCCCC CCGGGCCACC AGGACCCCCA GGCGAGAAGG GCTACATGGG CTTGGGCTTT
    CAAGGTCCCA AAGGTGAAAA GGGAGAACAA GGGGTCCAGG GTCCCCCGGG GCCACCAGGA
    CCAGCACCAC ACATGAAAGA AAAAGGAAGC GAAATCATAA AGGGAGAAAA GGGTGAGCCA
    GGTGAAAAGG GTGAACAGGG AGTCCCAGGA TTGCCAGGCT CAGGTTACAG AGGAGAAAAG
    GGTGAACCTG GGAAGCCCGG TCCACGTGGA AAGCCTGGAA AAGATGGTGA ACCAGGACCA
    AAAGGAGAAC CCGGCTTGCC TGGAGGGTTT GGGATTCCAG GAAGGCCTGG GGAACCTGGT
    GCTAAGGGTG AAAAAGGTGA TCAAGGTCCT CAAGGGCCTC CAGGAAGAAT TATAGATGCT
    GGACCAATAG ATACTTTTGG TGAAAAAGGA GACCCTGGAG TTTCAGGTTT GCCTGGACTA
    AAAGGTCAAA AAGGTGAAAA AGGTTTCCCT GGTGCACAAG GGATCCCAGG AATTCCAGGT
    CGACCAGTTC CAGACATGGT AGGATCACCT GGTGTCCCTG GCCAGAGAGG TGAAAAAGGT
    GAAAAGGGTT CTCCAGGATT CCCTATACCT GGACCTCCTG GAAGAGATGG TTTCCCAGGC
    CCACCCGGGT TGCCCGGCCC ACCAGGTCCT CCAGGTGTAA CAGATGGAAT AGATCAATGT
    CAGCAAGGGC AACCTGGTGC ACCAGGTGTA CCTGGACCTC CAGGGCAACA TGGATTTCCA
    GGAGAGATGG GAGAGAAAGG TGAAAAAGGT GAAACCTGTG CCTTGTGTGA TAAAGTAGGA
    CCCCCTGGAC TTCCAGGACC ACAAGGACTA CCGGGTCCAG CAGGTTTTCC GGGACAGTCA
    GGTTTCAAGG GTGACAGAGG CTTACCTGGA CTTGATGGCC TTCCAGGCGT ACCCGGGCCC
    CCAGGCGCTC CAGGACTCAT TGGCCTCCCT GGGGCAAAAG GAGAACCAGG AGACTTTACT
    TATGATTCCA TCCTGAAAGG TGAGAAGGGA GATCCTGGTT TCCCAGGACA ACCAGGTATT
    CCTGGAAGAG AAGGAACACC AGGAAAAGAC GGATTGCCAG GTCCTCAAGG TCCAAAAGGA
    GCATCGGGTA CAGCTGGCTT GAAAGGAGAA CGTGGTCCCC CTGGACAACC TGGATTCCCT
    GGAGTACGTG GTGAAAGAGG TTTACCTGGC TCTCCTGGGG TAGGCACTGC AGGGTTACCT
    GGTGACAAAG GAGAGAGAGG TTTTGCTGGA ACTCCAGGTT TACCAGGTCT TCCAGGAACA
    AAAGGTGAAG CAGGACGAAC TGTACCACTC CCTGGACCTC CTGGGGCAGA TGGCCTTCCT
    GGACCACCTG GTTTCCCTGG ACCCCAAGGT GACAAAGGGA ATCCTGGGCT TCCGGGGAGA
    CCTGGCCTGC CAGGAGAAAA AGGTGCTGTT GGGCAGCCAG GTATAGGGTT TCCTGGACCT
    CCAGGACCCA AAGGTTTCCC AGGTCAGCCT GGCTCACCTG GTCCTCCGGG TACTCCTGGA
    ACCCCTGGCC TGGATGGTTT TCCAGGCACA CCAGGTTTAC AAGGTCAAAA AGGTGAACCT
    GGAGTGGGTC TCCCTGGTCC TAAAGGGTTA CCAGGCATCC CAGGTCCAGC TGGCATCCCT
    GGAGAAAAAG GAAATCCAGG GCTGCCTGGC TTACGAGGTG AACAAGGATT CCCAGGTGTA
    CCTGGACAAC AAGGATTTAG AGGAGATCCA GGCCTCCCTG GACTACAGGG CTTGCCAGGC
    CCCCAGGGCT CACCAGGAGT GGGCCCACCG GGATTGCCAG GGCCCGCTGG GCAGCCAGGA
    CCTGAGGGGC CACCAGGATT TCCTGGAATA AAAGGAGAAA GGGGCTTCCC TGGTATCCCA
    GGTCTAGATA TGCCAGGGCC AAAAGGAGAT AAAGGTAGCC AGGGCCAGCC TGGAGTACCA
    GGGTCTGTGG GGCTACCTGG CCTGCCAGGT CCACAAGGAT CTGTTGGACT GAAAGGATCA
    GCAGGAGCCA AAGGAGAAAT GGGAGTTATG GGAACTCCAG GGTTGCCAGG GACTCCTGGA
    CCAGTTGGAG ATCGAGGGTT GCCTGGTGAA AAAGGTAGCC AAGGTTTGAA AGGTGTGGTT
    GGACCACAGG GTGAACCAGG TGTCAAAGGG GATAAAGGTG AAGCTGGTCT TCCGGGAATA
    CCTGGAAGCA TGGACACCAT GGACATGGTT AGTCTCAAAG GCCAAAAGGG AGATCCTGGA
    GAAAAAGGGG ACCTTGGTCC AACAGGAGAA AAAGGGTTAC GTGGGGAGTA TGGAGAACCA
    GGAATTCCAG GAAGGGATGG TGAACCTGGT ACTCCGGGAC AACCAGGACC AAAAGGAGAT
    CAAGGCCCCC CAGGGTACCC AGGAGATCCA GGAATTCCAG GGCAGAAAGG ATCAGTTGGT
    GAAATGGGTT TGCCAGGAAC ACCTGGAGAA AAGGGTCTGC CTGGAATACC AGGTTCACCA
    GGCACACCAG GGTTCCCTGG GCAAAAAGGT GAAAAAGGAG ACAAAGGAGC AGCAGGTTTT
    CCTGGAATTG GCTTTCCAGG ATCTCCTGGT GAGAAGGGAG AACCAGGAAG AACAGGCAGT
    CCGGGTTTGT CTGGAGACAA AGGTGAAAAG GGTAATGCAG GAATTCCAGG AATGCCGGGT
    GCTCCAGGTC CCAAAGGATC CCCTGGCATT GCAGGATTTC CAGGGAGCCC AGGCCTCCAT
    GGAGCAAAGG GTGAAAAAGG ACTTCCTGGC TTAAGTGGCA TCCCAGGCTT AAAAGGAGAA
    CCAGGTGAAC CTGGTGTTCC TGGTCCTGCT GGTTCCATTG GTCAGAAGGG TGAACCAGGC
    TATGATGGGA TTCCAGGCAC AGCTGGTGCA AAGGGTGAAC AAGGTGTACC AGGTAGAGGA
    GTTCCAGGAT ATCCAGGTGC AAAGGGAGAT AAAGGTGCAA AGGGTGAAGT GGGTTTCCCA
    GGATCCCCAG GAATTCCAGG GATTCCAGGC TTAAAAGGAG AACAAGGGTA TGCTGGCCCA
    CCCGGCCCTA AAGGCAACCA AGGTCTTCCT GGACTACCAG GAAGTGCAAT TGAGGGCCCT
    AAAGGAGACA GAGGACCACA AGGCCAACCC GGCCTTCCAG GTTTACCAGG TCCAACGGGG
    CCACCAGGAC CTCCAGGTCT GAAGGGAGCT AAAGGAGAGC AGGGAAGCGA TGGCTGGCCT
    GGAACTCCTG GAGGTCCAGG AGATAAAGGC GATCCAGGTT TCCAGGGACT GCCAGGTAGT
    CCTGGTTTGC CAGGAGGTGC TGGTGAAAAG GGTGATCTAG GGCCTCCAGG TGTTCCAGGT
    GTTCCAGGTC CAAAAGGATC TGCAGGCTTC CCAGGCCTCA AGGGTGAGCG AGGTGACCAA
    GGTCTTCCTG GATCTAAAGG TTTACAAGGC CCGCCGGGCC CCCCAGGTGC TTTTCAGCTT
    ATCAAAGGAG ATCCTGGCTT ACCTGGCCCG GTAGGACCAG TTGGTCTCAA AGGATTCCCA
    GGACATCCAG GTCCCAAAGG ACAGCAAGGA GTAACAGGGC CTTCAGGTGT ACCTGGACCA
    CCTGGTAGTC CAGGGTTTGA TGGCAGACCA GGGCAAAAAG GAGAAGTTGG TCCTTATGGT
    CCTCCAGGGC CCAGAGGAAT CCCTGGTCCG CCTGGCCCTG AAGGTTTGCC TGGGGCTATG
    GGTCCACCAG GTGCAGCATC TGTTGCTCAT GGCTTCCTTG TCACCAGACA CAGTCAAACC
    ACTGAAGAAC CACAATGTCC ATTTGGAACA AGGCTGATTT ACCATGGCTA CTCCCTTCTT
    TATGTACAAG GAAATGAAAG AGCACACGGT CAAGATCTGG GCACTGCAGG GAGCTGTCTT
    CGTAAATTTA GTACTATGCC TTTCTTATTT TGCAATATCA ACAATGTATG TAACTTTGCA
    TCAAGGAATG ACTATTCCTA CTGGCTGTCC ACTCCTGAAC CCATGCCAAT GAGTATGGCA
    CCTATCACTG GTGAAAGTAT CAGACCATTC ATCAGCAGAT GCTCTGTGTG TGAAGCTCCT
    GCTATGGTAA TCGCCGTTCA CAGTCAAACA ATCCAGATAC CACCATGTCC TGAAGGCTGG
    AGTTCCCTTT GGATTGGTTA CTCCTTTGTC ATGCACACCA GTGCTGGTGC TGAGGGCTCT
    GGCCAGGCCC TGGCGTCTCC TGGTTCCTGC CTGGAGGAGT TCCGCAGCGC TCCCTTCATT
    GAATGCCATG GCCGGGGCAC CTGCAATTAT TATGCAAATG CTTACAGCTT CTGGCTTGCC
    ACTATAGAGA GGAATGAGAT GTTCAAGAAA CCGACTCCGT CAACTTTGAA AGCAGGGGAT
    CTGCGCTCCA ACGTTAGCCG TTGTCAAGTC TGTATGAGAA ATACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025011789.1
    CDS307..5154
    Translation

    Target ORF information:

    RefSeq Version XM_025156021.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen type IV alpha 1 chain (COL4A1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025156021.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCAAGGAC CTGAAGGTCC CCCTGGGCCA CCAGGACTTA AGGGTGATAC TGGAGAACCA 
    GGGCTGCCAG GAACAAAAGG AACAAGAGGC CCACCAGGAC CATCAGGATT TCCAGGAAAC
    CCAGGCCTCC CTGGCATTCC TGGACAAGAT GGCCCTCCTG GTCCACCTGG CATTCCAGGA
    TGTAATGGCA CAAAGGGTGA AAGAGGTCCA GTAGGTCCCC CAGGTTTACC GGGACTGACT
    GGAAGCATAG GACCCCCAGG ACCTCCTGGA ATTAAGGGAG ATCCAGGTGA AGTGATTGGT
    ATTTTACCTC CTAGTGTGCT AAAAGGTGAT AAAGGCTTTC CTGGCCAACC TGGACTACCT
    GGTCCACCTG GAACTCCAGG GTTTCAGGGA CCAATGGGGC CACCAGGGAT TCCAGGAGAA
    CCAGGTCCCC CCGGGCCACC AGGACCCCCA GGCGAGAAGG GCTACATGGG CTTGGGCTTT
    CAAGGTCCCA AAGGTGAAAA GGGAGAACAA GGGGTCCAGG GTCCCCCGGG GCCACCAGGA
    CCAGCACCAC ACATGAAAGA AAAAGGAAGC GAAATCATAA AGGGAGAAAA GGGTGAGCCA
    GGTGAAAAGG GTGAACAGGG AGTCCCAGGA TTGCCAGGCT CAGGTTACAG AGGAGAAAAG
    GGTGAACCTG GGAAGCCCGG TCCACGTGGA AAGCCTGGAA AAGATGGTGA ACCAGGACCA
    AAAGGAGAAC CCGGCTTGCC TGGAGGGTTT GGGATTCCAG GAAGGCCTGG GGAACCTGGT
    GCTAAGGGTG AAAAAGGTGA TCAAGGTCCT CAAGGGCCTC CAGGAAGAAT TATAGATGCT
    GGACCAATAG ATACTTTTGG TGAAAAAGGA GACCCTGGAG TTTCAGGTTT GCCTGGACTA
    AAAGGTCAAA AAGGTGAAAA AGGTTTCCCT GGTGCACAAG GGATCCCAGG AATTCCAGGT
    CGACCAGTTC CAGACATGGT AGGATCACCT GGTGTCCCTG GCCAGAGAGG TGAAAAAGGT
    GAAAAGGGTT CTCCAGGATT CCCTATACCT GGACCTCCTG GAAGAGATGG TTTCCCAGGC
    CCACCCGGGT TGCCCGGCCC ACCAGGTCCT CCAGGTGTAA CAGATGGAAT AGATCAATGT
    CAGCAAGGGC AACCTGGTGC ACCAGGTGTA CCTGGACCTC CAGGGCAACA TGGATTTCCA
    GGAGAGATGG GAGAGAAAGG TGAAAAAGGT GAAACCTGTG CCTTGTGTGA TAAAGTAGGA
    CCCCCTGGAC TTCCAGGACC ACAAGGACTA CCGGGTCCAG CAGGTTTTCC GGGACAGTCA
    GGTTTCAAGG GTGACAGAGG CTTACCTGGA CTTGATGGCC TTCCAGGCGT ACCCGGGCCC
    CCAGGCGCTC CAGGACTCAT TGGCCTCCCT GGGGCAAAAG GAGAACCAGG AGACTTTACT
    TATGATTCCA TCCTGAAAGG TGAGAAGGGA GATCCTGGTT TCCCAGGACA ACCAGGTATT
    CCTGGAAGAG AAGGAACACC AGGAAAAGAC GGATTGCCAG GTCCTCAAGG TCCAAAAGGA
    GCATCGGGTA CAGCTGGCTT GAAAGGAGAA CGTGGTCCCC CTGGACAACC TGGATTCCCT
    GGAGTACGTG GTGAAAGAGG TTTACCTGGC TCTCCTGGGG TAGGCACTGC AGGGTTACCT
    GGTGACAAAG GAGAGAGAGG TTTTGCTGGA ACTCCAGGTT TACCAGGTCT TCCAGGAACA
    AAAGGTGAAG CAGGACGAAC TGTACCACTC CCTGGACCTC CTGGGGCAGA TGGCCTTCCT
    GGACCACCTG GTTTCCCTGG ACCCCAAGGT GACAAAGGGA ATCCTGGGCT TCCGGGGAGA
    CCTGGCCTGC CAGGAGAAAA AGGTGCTGTT GGGCAGCCAG GTATAGGGTT TCCTGGACCT
    CCAGGACCCA AAGGTTTCCC AGGTCAGCCT GGCTCACCTG GTCCTCCGGG TACTCCTGGA
    ACCCCTGGCC TGGATGGTTT TCCAGGCACA CCAGGTTTAC AAGGTCAAAA AGGTGAACCT
    GGAGTGGGTC TCCCTGGTCC TAAAGGGTTA CCAGGCATCC CAGGTCCAGC TGGCATCCCT
    GGAGAAAAAG GAAATCCAGG GCTGCCTGGC TTACGAGGTG AACAAGGATT CCCAGGTGTA
    CCTGGACAAC AAGGATTTAG AGGAGATCCA GGCCTCCCTG GACTACAGGG CTTGCCAGGC
    CCCCAGGGCT CACCAGGAGT GGGCCCACCG GGATTGCCAG GGCCCGCTGG GCAGCCAGGA
    CCTGAGGGGC CACCAGGATT TCCTGGAATA AAAGGAGAAA GGGGCTTCCC TGGTATCCCA
    GGTCTAGATA TGCCAGGGCC AAAAGGAGAT AAAGGTAGCC AGGGCCAGCC TGGAGTACCA
    GGGTCTGTGG GGCTACCTGG CCTGCCAGGT CCACAAGGAT CTGTTGGACT GAAAGGATCA
    GCAGGAGCCA AAGGAGAAAT GGGAGTTATG GGAACTCCAG GGTTGCCAGG GACTCCTGGA
    CCAGTTGGAG ATCGAGGGTT GCCTGGTGAA AAAGGTAGCC AAGGTTTGAA AGGTGTGGTT
    GGACCACAGG GTGAACCAGG TGTCAAAGGG GATAAAGGTG AAGCTGGTCT TCCGGGAATA
    CCTGGAAGCA TGGACACCAT GGACATGGTT AGTCTCAAAG GCCAAAAGGG AGATCCTGGA
    GAAAAAGGGG ACCTTGGTCC AACAGGAGAA AAAGGGTTAC GTGGGGAGTA TGGAGAACCA
    GGAATTCCAG GAAGGGATGG TGAACCTGGT ACTCCGGGAC AACCAGGACC AAAAGGAGAT
    CAAGGCCCCC CAGGGTACCC AGGAGATCCA GGAATTCCAG GGCAGAAAGG ATCAGTTGGT
    GAAATGGGTT TGCCAGGAAC ACCTGGAGAA AAGGGTCTGC CTGGAATACC AGGTTCACCA
    GGCACACCAG GGTTCCCTGG GCAAAAAGGT GAAAAAGGAG ACAAAGGAGC AGCAGGTTTT
    CCTGGAATTG GCTTTCCAGG ATCTCCTGGT GAGAAGGGAG AACCAGGAAG AACAGGCAGT
    CCGGGTTTGT CTGGAGACAA AGGTGAAAAG GGTAATGCAG GAATTCCAGG AATGCCGGGT
    GCTCCAGGTC CCAAAGGATC CCCTGGCATT GCAGGATTTC CAGGGAGCCC AGGCCTCCAT
    GGAGCAAAGG GTGAAAAAGG ACTTCCTGGC TTAAGTGGCA TCCCAGGCTT AAAAGGAGAA
    CCAGGTGAAC CTGGTGTTCC TGGTCCTGCT GGTTCCATTG GTCAGAAGGG TGAACCAGGC
    TATGATGGGA TTCCAGGCAC AGCTGGTGCA AAGGGTGAAC AAGGTGTACC AGGTAGAGGA
    GTTCCAGGAT ATCCAGGTGC AAAGGGAGAT AAAGGTGCAA AGGGTGAAGT GGGTTTCCCA
    GGATCCCCAG GAATTCCAGG GATTCCAGGC TTAAAAGGAG AACAAGGGTA TGCTGGCCCA
    CCCGGCCCTA AAGGCAACCA AGGTCTTCCT GGACTACCAG GAAGTGCAAT TGAGGGCCCT
    AAAGGAGACA GAGGACCACA AGGCCAACCC GGCCTTCCAG GTTTACCAGG TCCAACGGGG
    CCACCAGGAC CTCCAGGTCT GAAGGGAGCT AAAGGAGAGC AGGGAAGCGA TGGCTGGCCT
    GGAACTCCTG GAGGTCCAGG AGATAAAGGC GATCCAGGTT TCCAGGGACT GCCAGGTAGT
    CCTGGTTTGC CAGGAGGTGC TGGTGAAAAG GGTGATCTAG GGCCTCCAGG TGTTCCAGGT
    GTTCCAGGTC CAAAAGGATC TGCAGGCTTC CCAGGCCTCA AGGGTGAGCG AGGTGACCAA
    GGTCTTCCTG GATCTAAAGG TTTACAAGGC CCGCCGGGCC CCCCAGGTGC TTTTCAGCTT
    ATCAAAGGAG ATCCTGGCTT ACCTGGCCCG GTAGGACCAG TTGGTCTCAA AGGATTCCCA
    GGACATCCAG GTCCCAAAGG ACAGCAAGGA GTAACAGGGC CTTCAGGTGT ACCTGGACCA
    CCTGGTAGTC CAGGGTTTGA TGGCAGACCA GGGCAAAAAG GAGAAGTTGG TCCTTATGGT
    CCTCCAGGGC CCAGAGGAAT CCCTGGTCCG CCTGGCCCTG AAGGTTTGCC TGGGGCTATG
    GGTCCACCAG GTGCAGCATC TGTTGCTCAT GGCTTCCTTG TCACCAGACA CAGTCAAACC
    ACTGAAGAAC CACAATGTCC ATTTGGAACA AGGCTGATTT ACCATGGCTA CTCCCTTCTT
    TATGTACAAG GAAATGAAAG AGCACACGGT CAAGATCTGG GCACTGCAGG GAGCTGTCTT
    CGTAAATTTA GTACTATGCC TTTCTTATTT TGCAATATCA ACAATGTATG TAACTTTGCA
    TCAAGGAATG ACTATTCCTA CTGGCTGTCC ACTCCTGAAC CCATGCCAAT GAGTATGGCA
    CCTATCACTG GTGAAAGTAT CAGACCATTC ATCAGCAGAT GCTCTGTGTG TGAAGCTCCT
    GCTATGGTAA TCGCCGTTCA CAGTCAAACA ATCCAGATAC CACCATGTCC TGAAGGCTGG
    AGTTCCCTTT GGATTGGTTA CTCCTTTGTC ATGCACACCA GTGCTGGTGC TGAGGGCTCT
    GGCCAGGCCC TGGCGTCTCC TGGTTCCTGC CTGGAGGAGT TCCGCAGCGC TCCCTTCATT
    GAATGCCATG GCCGGGGCAC CTGCAATTAT TATGCAAATG CTTACAGCTT CTGGCTTGCC
    ACTATAGAGA GGAATGAGAT GTTCAAGAAA CCGACTCCGT CAACTTTGAA AGCAGGGGAT
    CTGCGCTCCA ACGTTAGCCG TTGTCAAGTC TGTATGAGAA ATACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa24130
    Clone ID Related Accession (Same CDS sequence) NM_001162399.3 , NM_001162399.2
    Accession Version NM_001162399.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5010bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-25
    Organism Gallus gallus(chicken)
    Product collagen alpha-1(IV) chain precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AADN05000386.1. On Jun 26, 2018 this sequence version replaced NM_001162399.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTGGCGTT GGCTGGGCTT CGGGCTGCTG CTGAGCGCCG AGCTCAGCGC CGTGTCCGCC 
    GCGGGTTGTT CTGGTTGTGG AAAATGTGAC TGCAGTGGAG TAAAAGGACA AAAGGGAGAA
    AGAGGCCTTC CGGGACTACA GGGTGTGATT GGGTTTCCTG GAATGCAAGG ACCTGAAGGT
    CCCCCTGGGC CACCAGGACT TAAGGGTGAT ACTGGAGAAC CAGGGCTGCC AGGAACAAAA
    GGAACAAGAG GCCCACCAGG ACCATCAGGA TTTCCAGGAA ACCCAGGCCT CCCTGGCATT
    CCTGGACAAG ATGGCCCTCC TGGTCCACCT GGCATTCCAG GATGTAATGG CACAAAGGGT
    GAAAGAGGTC CAGTAGGTCC CCCAGGTTTA CCGGGACTGA CTGGAAGCAT AGGACCCCCA
    GGACCTCCTG GAATTAAGGG AGATCCAGGT GAAGTGATTG GTATTTTACC TCCTAGTGTG
    CTAAAAGGTG ATAAAGGCTT TCCTGGCCAA CCTGGACTAC CTGGTCCACC TGGAACTCCA
    GGGTTTCAGG GACCAATGGG GCCACCAGGG ATTCCAGGAG AACCAGGTCC CCCCGGGCCA
    CCAGGACCCC CAGGCGAGAA GGGCTACATG GGCTTGGGCT TTCAAGGTCC CAAAGGTGAA
    AAGGGAGAAC AAGGGGTCCA GGGTCCCCCG GGGCCACCAG GACCAGCACC ACACATGAAA
    GAAAAAGGAA GCGAAATCAT AAAGGGAGAA AAGGGTGAGC CAGGTGAAAA GGGTGAACAG
    GGAGTCCCAG GATTGCCAGG CTCAGGTTAC AGAGGAGAAA AGGGTGAACC TGGGAAGCCC
    GGTCCACGTG GAAAGCCTGG AAAAGATGGT GAACCAGGAC CAAAAGGAGA ACCCGGCTTG
    CCTGGAGGGT TTGGGATTCC AGGAAGGCCT GGGGAACCTG GTGCTAAGGG TGAAAAAGGT
    GATCAAGGTC CTCAAGGGCC TCCAGGAAGA ATTATAGATG CTGGACCAAT AGATACTTTT
    GGTGAAAAAG GAGACCCTGG AGTTTCAGGT TTGCCTGGAC TAAAAGGTCA AAAAGGTGAA
    AAAGGTTTCC CTGGTGCACA AGGGATCCCA GGAATTCCAG GTCGACCAGT TCCAGACATG
    GTAGGATCAC CTGGTGTCCC TGGCCAGAGA GGTGAAAAAG GTGAAAAGGG TTCTCCAGGA
    TTCCCTATAC CTGGACCTCC TGGAAGAGAT GGTTTCCCAG GCCCACCCGG GTTGCCCGGC
    CCACCAGGTC CTCCAGGTGT AACAGATGGA ATAGATCAAT GTCAGCAAGG GCAACCTGGT
    GCACCAGGTG TACCTGGACC TCCAGGGCAA CATGGATTTC CAGGAGAGAT GGGAGAGAAA
    GGTGAAAAAG GTGAAACCTG TGCCTTGTGT GATAAAGTAG GACCCCCTGG ACTTCCAGGA
    CCACAAGGAC TACCGGGTCC AGCAGGTTTT CCGGGACAGT CAGGTTTCAA GGGTGACAGA
    GGCTTACCTG GACTTGATGG CCTTCCAGGC GTACCCGGGC CCCCAGGCGC TCCAGGACTC
    ATTGGCCTCC CTGGGGCAAA AGGAGAACCA GGAGACTTTA CTTATGATTC CATCCTGAAA
    GGTGAGAAGG GAGATCCTGG TTTCCCAGGA CAACCAGGTA TTCCTGGAAG AGAAGGAACA
    CCAGGAAAAG ACGGATTGCC AGGTCCTCAA GGTCCAAAAG GAGCATCGGG TACAGCTGGC
    TTGAAAGGAG AACGTGGTCC CCCTGGACAA CCTGGATTCC CTGGAGTACG TGGTGAAAGA
    GGTTTACCTG GCTCTCCTGG GGTAGGCACT GCAGGGTTAC CTGGTGACAA AGGAGAGAGA
    GGTTTTGCTG GAACTCCAGG TTTACCAGGT CTTCCAGGAA CAAAAGGTGA AGCAGGACGA
    ACTGTACCAC TCCCTGGACC TCCTGGGGCA GATGGCCTTC CTGGACCACC TGGTTTCCCT
    GGACCCCAAG GTGACAAAGG GAATCCTGGG CTTCCGGGGA GACCTGGCCT GCCAGGAGAA
    AAAGGTGCTG TTGGGCAGCC AGGTATAGGG TTTCCTGGAC CTCCAGGACC CAAAGGTTTC
    CCAGGTCAGC CTGGCTCACC TGGTCCTCCG GGTACTCCTG GAACCCCTGG CCTGGATGGT
    TTTCCAGGCA CACCAGGTTT ACAAGGTCAA AAAGGTGAAC CTGGAGTGGG TCTCCCTGGT
    CCTAAAGGGT TACCAGGCAT CCCAGGTCCA GCTGGCATCC CTGGAGAAAA AGGAAATCCA
    GGGCTGCCTG GCTTACGAGG TGAACAAGGA TTCCCAGGTG TACCTGGACA ACAAGGATTT
    AGAGGAGATC CAGGCCTCCC TGGACTACAG GGCTTGCCAG GCCCCCAGGG CTCACCAGGA
    GTGGGCCCAC CGGGATTGCC AGGGCCCGCT GGGCAGCCAG GACCTGAGGG GCCACCAGGA
    TTTCCTGGAA TAAAAGGAGA AAGGGGCTTC CCTGGTATCC CAGGTCTAGA TATGCCAGGG
    CCAAAAGGAG ATAAAGGTAG CCAGGGCCAG CCTGGAGTAC CAGGGTCTGT GGGGCTACCT
    GGCCTGCCAG GTCCACAAGG ATCTGTTGGA CTGAAAGGAT CAGCAGGAGC CAAAGGAGAA
    ATGGGAGTTA TGGGAACTCC AGGGTTGCCA GGGACTCCTG GACCAGTTGG AGATCGAGGG
    TTGCCTGGTG AAAAAGGTAG CCAAGGTTTG AAAGGTGTGG TTGGACCACA GGGTGAACCA
    GGTGTCAAAG GGGATAAAGG TGAAGCTGGT CTTCCGGGAA TACCTGGAAG CATGGACACC
    ATGGACATGG TTAGTCTCAA AGGCCAAAAG GGAGATCCTG GAGAAAAAGG GGACCTTGGT
    CCAACAGGAG AAAAAGGGTT ACGTGGGGAG TATGGAGAAC CAGGAATTCC AGGAAGGGAT
    GGTGAACCTG GTACTCCGGG ACAACCAGGA CCAAAAGGAG ATCAAGGCCC CCCAGGGTAC
    CCAGGAGATC CAGGAATTCC AGGGCAGAAA GGATCAGTTG GTGAAATGGG TTTGCCAGGA
    ACACCTGGAG AAAAGGGTCT GCCTGGAATA CCAGGTTCAC CAGGCACACC AGGGTTCCCT
    GGGCAAAAAG GTGAAAAAGG AGACAAAGGA GCAGCAGGTT TTCCTGGAAT TGGCTTTCCA
    GGATCTCCTG GTGAGAAGGG AGAACCAGGA AGAACAGGCA GTCCGGGTTT GTCTGGAGAC
    AAAGGTGAAA AGGGTAATGC AGGAATTCCA GGAATGCCGG GTGCTCCAGG TCCCAAAGGA
    TCCCCTGGCA TTGCAGGATT TCCAGGCAAC CCAGGCCTCC ATGGAGCAAA GGGTGAAAAA
    GGACTTCCTG GCTTAAGTGG CATCCCAGGC TTAAAAGGAG AACCAGGTGA ACCTGGTGTT
    CCTGGTCCTG CTGGTTCCAT TGGTCAGAAG GGTGAACCAG GCTATGATGG GATTCCAGGC
    ACAGCTGGTG CAAAGGGTGA ACAAGGTGTA CCAGGTAGAG GAGTTCCAGG ATATCCAGGT
    GCAAAGGGAG ATAAAGGTGC AAAGGGTGAA GTGGGTTTCC CAGGATCCCC AGGAATTCCA
    GGGATTCCAG GCTTAAAAGG AGAACAAGGG TATGCTGGCC CACCCGGCCC TAAAGGCAAC
    CAAGGTCTTC CTGGACTACC AGGAAGTGCA ATTGAGGGCC CTAAAGGAGA CAGAGGACCA
    CAAGGCCAAC CCGGCCTTCC AGGTTTACCA GGTCCAACGG GGCCACCAGG ACCTCCAGGT
    CTGAAGGGAG CTAAAGGAGA GCAGGGAAGC GATGGCTGGC CTGGAACTCC TGGAGGTCCA
    GGAGATAAAG GCGATCCAGG TTTCCAGGGA CTGCCAGGTA GTCCTGGTTT GCCAGGAGGT
    GCTGGTGAAA AGGGTGATCT AGGGCCTCCA GGTGTTCCAG GTGTTCCAGG TCCAAAAGGA
    TCTGCAGGCT TCCCAGGCCT CAAGGGTGAG CGAGGTGACC AAGGTCTTCC TGGATCTAAA
    GGTTTACAAG GCCCGCCGGG CCCCCCAGGT GCTTTTCAGC TTATCAAAGG AGATCCTGGC
    TTACCTGGCC CGGTAGGACC AGTTGGTCTC AAAGGATTCC CAGGACATCC AGGTCCCAAA
    GGACAGCAAG GAGTAACAGG GCCTTCAGGT GTACCTGGAC CACCTGGTAG TCCAGGGTTT
    GATGGCAGAC CAGGGCAAAA AGGAGAAGTT GGTCCTTATG GTCCTCCAGG GCCCAGAGGA
    ATCCCTGGTC CGCCTGGCCC TGAAGGTTTG CCTGGGGCTA TGGGTCCACC AGGTGCAGCA
    TCTGTTGCTC ATGGCTTCCT TGTCACCAGA CACAGTCAAA CCACTGAAGA ACCACAATGT
    CCATTTGGAA CAAGGCTGAT TTACCATGGC TACTCCCTTC TTTATGTACA AGGAAATGAA
    AGAGCACACG GTCAAGATCT GGGCACTGCA GGGAGCTGTC TTCGTAAATT TAGTACTATG
    CCTTTCTTAT TTTGCAATAT CAACAATGTA TGTAACTTTG CATCAAGGAA TGACTATTCC
    TACTGGCTGT CCACTCCTGA ACCCATGCCA ATGAGTATGG CACCTATCAC TGGTGAAAGT
    ATCAGACCAT TCATCAGCAG ATGCTCTGTG TGTGAAGCTC CTGCTATGGT AATCGCCGTT
    CACAGTCAAA CAATCCAGAT ACCACCATGT CCTGAAGGCT GGAGTTCCCT TTGGATTGGT
    TACTCCTTTG TCATGCACAC CAGTGCTGGT GCTGAGGGCT CTGGCCAGGC CCTGGCGTCT
    CCTGGTTCCT GCCTGGAGGA GTTCCGCAGC GCTCCCTTCA TTGAATGCCA TGGCCGGGGC
    ACCTGCAATT ATTATGCAAA TGCTTACAGC TTCTGGCTTG CCACTATAGA GAGGAATGAG
    ATGTTCAAGA AACCGACTCC GTCAACTTTG AAAGCAGGGG ATCTGCGCTC CAACGTTAGC
    CGTTGTCAAG TCTGTATGAG AAATACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001155871.1
    CDS127..5136
    Translation

    Target ORF information:

    RefSeq Version NM_001162399.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen type IV alpha 1 chain (COL4A1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001162399.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTGGCGTT GGCTGGGCTT CGGGCTGCTG CTGAGCGCCG AGCTCAGCGC CGTGTCCGCC 
    GCGGGTTGTT CTGGTTGTGG AAAATGTGAC TGCAGTGGAG TAAAAGGACA AAAGGGAGAA
    AGAGGCCTTC CGGGACTACA GGGTGTGATT GGGTTTCCTG GAATGCAAGG ACCTGAAGGT
    CCCCCTGGGC CACCAGGACT TAAGGGTGAT ACTGGAGAAC CAGGGCTGCC AGGAACAAAA
    GGAACAAGAG GCCCACCAGG ACCATCAGGA TTTCCAGGAA ACCCAGGCCT CCCTGGCATT
    CCTGGACAAG ATGGCCCTCC TGGTCCACCT GGCATTCCAG GATGTAATGG CACAAAGGGT
    GAAAGAGGTC CAGTAGGTCC CCCAGGTTTA CCGGGACTGA CTGGAAGCAT AGGACCCCCA
    GGACCTCCTG GAATTAAGGG AGATCCAGGT GAAGTGATTG GTATTTTACC TCCTAGTGTG
    CTAAAAGGTG ATAAAGGCTT TCCTGGCCAA CCTGGACTAC CTGGTCCACC TGGAACTCCA
    GGGTTTCAGG GACCAATGGG GCCACCAGGG ATTCCAGGAG AACCAGGTCC CCCCGGGCCA
    CCAGGACCCC CAGGCGAGAA GGGCTACATG GGCTTGGGCT TTCAAGGTCC CAAAGGTGAA
    AAGGGAGAAC AAGGGGTCCA GGGTCCCCCG GGGCCACCAG GACCAGCACC ACACATGAAA
    GAAAAAGGAA GCGAAATCAT AAAGGGAGAA AAGGGTGAGC CAGGTGAAAA GGGTGAACAG
    GGAGTCCCAG GATTGCCAGG CTCAGGTTAC AGAGGAGAAA AGGGTGAACC TGGGAAGCCC
    GGTCCACGTG GAAAGCCTGG AAAAGATGGT GAACCAGGAC CAAAAGGAGA ACCCGGCTTG
    CCTGGAGGGT TTGGGATTCC AGGAAGGCCT GGGGAACCTG GTGCTAAGGG TGAAAAAGGT
    GATCAAGGTC CTCAAGGGCC TCCAGGAAGA ATTATAGATG CTGGACCAAT AGATACTTTT
    GGTGAAAAAG GAGACCCTGG AGTTTCAGGT TTGCCTGGAC TAAAAGGTCA AAAAGGTGAA
    AAAGGTTTCC CTGGTGCACA AGGGATCCCA GGAATTCCAG GTCGACCAGT TCCAGACATG
    GTAGGATCAC CTGGTGTCCC TGGCCAGAGA GGTGAAAAAG GTGAAAAGGG TTCTCCAGGA
    TTCCCTATAC CTGGACCTCC TGGAAGAGAT GGTTTCCCAG GCCCACCCGG GTTGCCCGGC
    CCACCAGGTC CTCCAGGTGT AACAGATGGA ATAGATCAAT GTCAGCAAGG GCAACCTGGT
    GCACCAGGTG TACCTGGACC TCCAGGGCAA CATGGATTTC CAGGAGAGAT GGGAGAGAAA
    GGTGAAAAAG GTGAAACCTG TGCCTTGTGT GATAAAGTAG GACCCCCTGG ACTTCCAGGA
    CCACAAGGAC TACCGGGTCC AGCAGGTTTT CCGGGACAGT CAGGTTTCAA GGGTGACAGA
    GGCTTACCTG GACTTGATGG CCTTCCAGGC GTACCCGGGC CCCCAGGCGC TCCAGGACTC
    ATTGGCCTCC CTGGGGCAAA AGGAGAACCA GGAGACTTTA CTTATGATTC CATCCTGAAA
    GGTGAGAAGG GAGATCCTGG TTTCCCAGGA CAACCAGGTA TTCCTGGAAG AGAAGGAACA
    CCAGGAAAAG ACGGATTGCC AGGTCCTCAA GGTCCAAAAG GAGCATCGGG TACAGCTGGC
    TTGAAAGGAG AACGTGGTCC CCCTGGACAA CCTGGATTCC CTGGAGTACG TGGTGAAAGA
    GGTTTACCTG GCTCTCCTGG GGTAGGCACT GCAGGGTTAC CTGGTGACAA AGGAGAGAGA
    GGTTTTGCTG GAACTCCAGG TTTACCAGGT CTTCCAGGAA CAAAAGGTGA AGCAGGACGA
    ACTGTACCAC TCCCTGGACC TCCTGGGGCA GATGGCCTTC CTGGACCACC TGGTTTCCCT
    GGACCCCAAG GTGACAAAGG GAATCCTGGG CTTCCGGGGA GACCTGGCCT GCCAGGAGAA
    AAAGGTGCTG TTGGGCAGCC AGGTATAGGG TTTCCTGGAC CTCCAGGACC CAAAGGTTTC
    CCAGGTCAGC CTGGCTCACC TGGTCCTCCG GGTACTCCTG GAACCCCTGG CCTGGATGGT
    TTTCCAGGCA CACCAGGTTT ACAAGGTCAA AAAGGTGAAC CTGGAGTGGG TCTCCCTGGT
    CCTAAAGGGT TACCAGGCAT CCCAGGTCCA GCTGGCATCC CTGGAGAAAA AGGAAATCCA
    GGGCTGCCTG GCTTACGAGG TGAACAAGGA TTCCCAGGTG TACCTGGACA ACAAGGATTT
    AGAGGAGATC CAGGCCTCCC TGGACTACAG GGCTTGCCAG GCCCCCAGGG CTCACCAGGA
    GTGGGCCCAC CGGGATTGCC AGGGCCCGCT GGGCAGCCAG GACCTGAGGG GCCACCAGGA
    TTTCCTGGAA TAAAAGGAGA AAGGGGCTTC CCTGGTATCC CAGGTCTAGA TATGCCAGGG
    CCAAAAGGAG ATAAAGGTAG CCAGGGCCAG CCTGGAGTAC CAGGGTCTGT GGGGCTACCT
    GGCCTGCCAG GTCCACAAGG ATCTGTTGGA CTGAAAGGAT CAGCAGGAGC CAAAGGAGAA
    ATGGGAGTTA TGGGAACTCC AGGGTTGCCA GGGACTCCTG GACCAGTTGG AGATCGAGGG
    TTGCCTGGTG AAAAAGGTAG CCAAGGTTTG AAAGGTGTGG TTGGACCACA GGGTGAACCA
    GGTGTCAAAG GGGATAAAGG TGAAGCTGGT CTTCCGGGAA TACCTGGAAG CATGGACACC
    ATGGACATGG TTAGTCTCAA AGGCCAAAAG GGAGATCCTG GAGAAAAAGG GGACCTTGGT
    CCAACAGGAG AAAAAGGGTT ACGTGGGGAG TATGGAGAAC CAGGAATTCC AGGAAGGGAT
    GGTGAACCTG GTACTCCGGG ACAACCAGGA CCAAAAGGAG ATCAAGGCCC CCCAGGGTAC
    CCAGGAGATC CAGGAATTCC AGGGCAGAAA GGATCAGTTG GTGAAATGGG TTTGCCAGGA
    ACACCTGGAG AAAAGGGTCT GCCTGGAATA CCAGGTTCAC CAGGCACACC AGGGTTCCCT
    GGGCAAAAAG GTGAAAAAGG AGACAAAGGA GCAGCAGGTT TTCCTGGAAT TGGCTTTCCA
    GGATCTCCTG GTGAGAAGGG AGAACCAGGA AGAACAGGCA GTCCGGGTTT GTCTGGAGAC
    AAAGGTGAAA AGGGTAATGC AGGAATTCCA GGAATGCCGG GTGCTCCAGG TCCCAAAGGA
    TCCCCTGGCA TTGCAGGATT TCCAGGCAAC CCAGGCCTCC ATGGAGCAAA GGGTGAAAAA
    GGACTTCCTG GCTTAAGTGG CATCCCAGGC TTAAAAGGAG AACCAGGTGA ACCTGGTGTT
    CCTGGTCCTG CTGGTTCCAT TGGTCAGAAG GGTGAACCAG GCTATGATGG GATTCCAGGC
    ACAGCTGGTG CAAAGGGTGA ACAAGGTGTA CCAGGTAGAG GAGTTCCAGG ATATCCAGGT
    GCAAAGGGAG ATAAAGGTGC AAAGGGTGAA GTGGGTTTCC CAGGATCCCC AGGAATTCCA
    GGGATTCCAG GCTTAAAAGG AGAACAAGGG TATGCTGGCC CACCCGGCCC TAAAGGCAAC
    CAAGGTCTTC CTGGACTACC AGGAAGTGCA ATTGAGGGCC CTAAAGGAGA CAGAGGACCA
    CAAGGCCAAC CCGGCCTTCC AGGTTTACCA GGTCCAACGG GGCCACCAGG ACCTCCAGGT
    CTGAAGGGAG CTAAAGGAGA GCAGGGAAGC GATGGCTGGC CTGGAACTCC TGGAGGTCCA
    GGAGATAAAG GCGATCCAGG TTTCCAGGGA CTGCCAGGTA GTCCTGGTTT GCCAGGAGGT
    GCTGGTGAAA AGGGTGATCT AGGGCCTCCA GGTGTTCCAG GTGTTCCAGG TCCAAAAGGA
    TCTGCAGGCT TCCCAGGCCT CAAGGGTGAG CGAGGTGACC AAGGTCTTCC TGGATCTAAA
    GGTTTACAAG GCCCGCCGGG CCCCCCAGGT GCTTTTCAGC TTATCAAAGG AGATCCTGGC
    TTACCTGGCC CGGTAGGACC AGTTGGTCTC AAAGGATTCC CAGGACATCC AGGTCCCAAA
    GGACAGCAAG GAGTAACAGG GCCTTCAGGT GTACCTGGAC CACCTGGTAG TCCAGGGTTT
    GATGGCAGAC CAGGGCAAAA AGGAGAAGTT GGTCCTTATG GTCCTCCAGG GCCCAGAGGA
    ATCCCTGGTC CGCCTGGCCC TGAAGGTTTG CCTGGGGCTA TGGGTCCACC AGGTGCAGCA
    TCTGTTGCTC ATGGCTTCCT TGTCACCAGA CACAGTCAAA CCACTGAAGA ACCACAATGT
    CCATTTGGAA CAAGGCTGAT TTACCATGGC TACTCCCTTC TTTATGTACA AGGAAATGAA
    AGAGCACACG GTCAAGATCT GGGCACTGCA GGGAGCTGTC TTCGTAAATT TAGTACTATG
    CCTTTCTTAT TTTGCAATAT CAACAATGTA TGTAACTTTG CATCAAGGAA TGACTATTCC
    TACTGGCTGT CCACTCCTGA ACCCATGCCA ATGAGTATGG CACCTATCAC TGGTGAAAGT
    ATCAGACCAT TCATCAGCAG ATGCTCTGTG TGTGAAGCTC CTGCTATGGT AATCGCCGTT
    CACAGTCAAA CAATCCAGAT ACCACCATGT CCTGAAGGCT GGAGTTCCCT TTGGATTGGT
    TACTCCTTTG TCATGCACAC CAGTGCTGGT GCTGAGGGCT CTGGCCAGGC CCTGGCGTCT
    CCTGGTTCCT GCCTGGAGGA GTTCCGCAGC GCTCCCTTCA TTGAATGCCA TGGCCGGGGC
    ACCTGCAATT ATTATGCAAA TGCTTACAGC TTCTGGCTTG CCACTATAGA GAGGAATGAG
    ATGTTCAAGA AACCGACTCC GTCAACTTTG AAAGCAGGGG ATCTGCGCTC CAACGTTAGC
    CGTTGTCAAG TCTGTATGAG AAATACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa24130
    Clone ID Related Accession (Same CDS sequence) NM_001162399.3 , NM_001162399.2
    Accession Version NM_001162399.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5010bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-11-05
    Organism Gallus gallus(chicken)
    Product collagen alpha-1(IV) chain precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AADN04000282.1. On Feb 10, 2016 this sequence version replaced NM_001162399.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTGGCGTT GGCTGGGCTT CGGGCTGCTG CTGAGCGCCG AGCTCAGCGC CGTGTCCGCC 
    GCGGGTTGTT CTGGTTGTGG AAAATGTGAC TGCAGTGGAG TAAAAGGACA AAAGGGAGAA
    AGAGGCCTTC CGGGACTACA GGGTGTGATT GGGTTTCCTG GAATGCAAGG ACCTGAAGGT
    CCCCCTGGGC CACCAGGACT TAAGGGTGAT ACTGGAGAAC CAGGGCTGCC AGGAACAAAA
    GGAACAAGAG GCCCACCAGG ACCATCAGGA TTTCCAGGAA ACCCAGGCCT CCCTGGCATT
    CCTGGACAAG ATGGCCCTCC TGGTCCACCT GGCATTCCAG GATGTAATGG CACAAAGGGT
    GAAAGAGGTC CAGTAGGTCC CCCAGGTTTA CCGGGACTGA CTGGAAGCAT AGGACCCCCA
    GGACCTCCTG GAATTAAGGG AGATCCAGGT GAAGTGATTG GTATTTTACC TCCTAGTGTG
    CTAAAAGGTG ATAAAGGCTT TCCTGGCCAA CCTGGACTAC CTGGTCCACC TGGAACTCCA
    GGGTTTCAGG GACCAATGGG GCCACCAGGG ATTCCAGGAG AACCAGGTCC CCCCGGGCCA
    CCAGGACCCC CAGGCGAGAA GGGCTACATG GGCTTGGGCT TTCAAGGTCC CAAAGGTGAA
    AAGGGAGAAC AAGGGGTCCA GGGTCCCCCG GGGCCACCAG GACCAGCACC ACACATGAAA
    GAAAAAGGAA GCGAAATCAT AAAGGGAGAA AAGGGTGAGC CAGGTGAAAA GGGTGAACAG
    GGAGTCCCAG GATTGCCAGG CTCAGGTTAC AGAGGAGAAA AGGGTGAACC TGGGAAGCCC
    GGTCCACGTG GAAAGCCTGG AAAAGATGGT GAACCAGGAC CAAAAGGAGA ACCCGGCTTG
    CCTGGAGGGT TTGGGATTCC AGGAAGGCCT GGGGAACCTG GTGCTAAGGG TGAAAAAGGT
    GATCAAGGTC CTCAAGGGCC TCCAGGAAGA ATTATAGATG CTGGACCAAT AGATACTTTT
    GGTGAAAAAG GAGACCCTGG AGTTTCAGGT TTGCCTGGAC TAAAAGGTCA AAAAGGTGAA
    AAAGGTTTCC CTGGTGCACA AGGGATCCCA GGAATTCCAG GTCGACCAGT TCCAGACATG
    GTAGGATCAC CTGGTGTCCC TGGCCAGAGA GGTGAAAAAG GTGAAAAGGG TTCTCCAGGA
    TTCCCTATAC CTGGACCTCC TGGAAGAGAT GGTTTCCCAG GCCCACCCGG GTTGCCCGGC
    CCACCAGGTC CTCCAGGTGT AACAGATGGA ATAGATCAAT GTCAGCAAGG GCAACCTGGT
    GCACCAGGTG TACCTGGACC TCCAGGGCAA CATGGATTTC CAGGAGAGAT GGGAGAGAAA
    GGTGAAAAAG GTGAAACCTG TGCCTTGTGT GATAAAGTAG GACCCCCTGG ACTTCCAGGA
    CCACAAGGAC TACCGGGTCC AGCAGGTTTT CCGGGACAGT CAGGTTTCAA GGGTGACAGA
    GGCTTACCTG GACTTGATGG CCTTCCAGGC GTACCCGGGC CCCCAGGCGC TCCAGGACTC
    ATTGGCCTCC CTGGGGCAAA AGGAGAACCA GGAGACTTTA CTTATGATTC CATCCTGAAA
    GGTGAGAAGG GAGATCCTGG TTTCCCAGGA CAACCAGGTA TTCCTGGAAG AGAAGGAACA
    CCAGGAAAAG ACGGATTGCC AGGTCCTCAA GGTCCAAAAG GAGCATCGGG TACAGCTGGC
    TTGAAAGGAG AACGTGGTCC CCCTGGACAA CCTGGATTCC CTGGAGTACG TGGTGAAAGA
    GGTTTACCTG GCTCTCCTGG GGTAGGCACT GCAGGGTTAC CTGGTGACAA AGGAGAGAGA
    GGTTTTGCTG GAACTCCAGG TTTACCAGGT CTTCCAGGAA CAAAAGGTGA AGCAGGACGA
    ACTGTACCAC TCCCTGGACC TCCTGGGGCA GATGGCCTTC CTGGACCACC TGGTTTCCCT
    GGACCCCAAG GTGACAAAGG GAATCCTGGG CTTCCGGGGA GACCTGGCCT GCCAGGAGAA
    AAAGGTGCTG TTGGGCAGCC AGGTATAGGG TTTCCTGGAC CTCCAGGACC CAAAGGTTTC
    CCAGGTCAGC CTGGCTCACC TGGTCCTCCG GGTACTCCTG GAACCCCTGG CCTGGATGGT
    TTTCCAGGCA CACCAGGTTT ACAAGGTCAA AAAGGTGAAC CTGGAGTGGG TCTCCCTGGT
    CCTAAAGGGT TACCAGGCAT CCCAGGTCCA GCTGGCATCC CTGGAGAAAA AGGAAATCCA
    GGGCTGCCTG GCTTACGAGG TGAACAAGGA TTCCCAGGTG TACCTGGACA ACAAGGATTT
    AGAGGAGATC CAGGCCTCCC TGGACTACAG GGCTTGCCAG GCCCCCAGGG CTCACCAGGA
    GTGGGCCCAC CGGGATTGCC AGGGCCCGCT GGGCAGCCAG GACCTGAGGG GCCACCAGGA
    TTTCCTGGAA TAAAAGGAGA AAGGGGCTTC CCTGGTATCC CAGGTCTAGA TATGCCAGGG
    CCAAAAGGAG ATAAAGGTAG CCAGGGCCAG CCTGGAGTAC CAGGGTCTGT GGGGCTACCT
    GGCCTGCCAG GTCCACAAGG ATCTGTTGGA CTGAAAGGAT CAGCAGGAGC CAAAGGAGAA
    ATGGGAGTTA TGGGAACTCC AGGGTTGCCA GGGACTCCTG GACCAGTTGG AGATCGAGGG
    TTGCCTGGTG AAAAAGGTAG CCAAGGTTTG AAAGGTGTGG TTGGACCACA GGGTGAACCA
    GGTGTCAAAG GGGATAAAGG TGAAGCTGGT CTTCCGGGAA TACCTGGAAG CATGGACACC
    ATGGACATGG TTAGTCTCAA AGGCCAAAAG GGAGATCCTG GAGAAAAAGG GGACCTTGGT
    CCAACAGGAG AAAAAGGGTT ACGTGGGGAG TATGGAGAAC CAGGAATTCC AGGAAGGGAT
    GGTGAACCTG GTACTCCGGG ACAACCAGGA CCAAAAGGAG ATCAAGGCCC CCCAGGGTAC
    CCAGGAGATC CAGGAATTCC AGGGCAGAAA GGATCAGTTG GTGAAATGGG TTTGCCAGGA
    ACACCTGGAG AAAAGGGTCT GCCTGGAATA CCAGGTTCAC CAGGCACACC AGGGTTCCCT
    GGGCAAAAAG GTGAAAAAGG AGACAAAGGA GCAGCAGGTT TTCCTGGAAT TGGCTTTCCA
    GGATCTCCTG GTGAGAAGGG AGAACCAGGA AGAACAGGCA GTCCGGGTTT GTCTGGAGAC
    AAAGGTGAAA AGGGTAATGC AGGAATTCCA GGAATGCCGG GTGCTCCAGG TCCCAAAGGA
    TCCCCTGGCA TTGCAGGATT TCCAGGCAAC CCAGGCCTCC ATGGAGCAAA GGGTGAAAAA
    GGACTTCCTG GCTTAAGTGG CATCCCAGGC TTAAAAGGAG AACCAGGTGA ACCTGGTGTT
    CCTGGTCCTG CTGGTTCCAT TGGTCAGAAG GGTGAACCAG GCTATGATGG GATTCCAGGC
    ACAGCTGGTG CAAAGGGTGA ACAAGGTGTA CCAGGTAGAG GAGTTCCAGG ATATCCAGGT
    GCAAAGGGAG ATAAAGGTGC AAAGGGTGAA GTGGGTTTCC CAGGATCCCC AGGAATTCCA
    GGGATTCCAG GCTTAAAAGG AGAACAAGGG TATGCTGGCC CACCCGGCCC TAAAGGCAAC
    CAAGGTCTTC CTGGACTACC AGGAAGTGCA ATTGAGGGCC CTAAAGGAGA CAGAGGACCA
    CAAGGCCAAC CCGGCCTTCC AGGTTTACCA GGTCCAACGG GGCCACCAGG ACCTCCAGGT
    CTGAAGGGAG CTAAAGGAGA GCAGGGAAGC GATGGCTGGC CTGGAACTCC TGGAGGTCCA
    GGAGATAAAG GCGATCCAGG TTTCCAGGGA CTGCCAGGTA GTCCTGGTTT GCCAGGAGGT
    GCTGGTGAAA AGGGTGATCT AGGGCCTCCA GGTGTTCCAG GTGTTCCAGG TCCAAAAGGA
    TCTGCAGGCT TCCCAGGCCT CAAGGGTGAG CGAGGTGACC AAGGTCTTCC TGGATCTAAA
    GGTTTACAAG GCCCGCCGGG CCCCCCAGGT GCTTTTCAGC TTATCAAAGG AGATCCTGGC
    TTACCTGGCC CGGTAGGACC AGTTGGTCTC AAAGGATTCC CAGGACATCC AGGTCCCAAA
    GGACAGCAAG GAGTAACAGG GCCTTCAGGT GTACCTGGAC CACCTGGTAG TCCAGGGTTT
    GATGGCAGAC CAGGGCAAAA AGGAGAAGTT GGTCCTTATG GTCCTCCAGG GCCCAGAGGA
    ATCCCTGGTC CGCCTGGCCC TGAAGGTTTG CCTGGGGCTA TGGGTCCACC AGGTGCAGCA
    TCTGTTGCTC ATGGCTTCCT TGTCACCAGA CACAGTCAAA CCACTGAAGA ACCACAATGT
    CCATTTGGAA CAAGGCTGAT TTACCATGGC TACTCCCTTC TTTATGTACA AGGAAATGAA
    AGAGCACACG GTCAAGATCT GGGCACTGCA GGGAGCTGTC TTCGTAAATT TAGTACTATG
    CCTTTCTTAT TTTGCAATAT CAACAATGTA TGTAACTTTG CATCAAGGAA TGACTATTCC
    TACTGGCTGT CCACTCCTGA ACCCATGCCA ATGAGTATGG CACCTATCAC TGGTGAAAGT
    ATCAGACCAT TCATCAGCAG ATGCTCTGTG TGTGAAGCTC CTGCTATGGT AATCGCCGTT
    CACAGTCAAA CAATCCAGAT ACCACCATGT CCTGAAGGCT GGAGTTCCCT TTGGATTGGT
    TACTCCTTTG TCATGCACAC CAGTGCTGGT GCTGAGGGCT CTGGCCAGGC CCTGGCGTCT
    CCTGGTTCCT GCCTGGAGGA GTTCCGCAGC GCTCCCTTCA TTGAATGCCA TGGCCGGGGC
    ACCTGCAATT ATTATGCAAA TGCTTACAGC TTCTGGCTTG CCACTATAGA GAGGAATGAG
    ATGTTCAAGA AACCGACTCC GTCAACTTTG AAAGCAGGGG ATCTGCGCTC CAACGTTAGC
    CGTTGTCAAG TCTGTATGAG AAATACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001155871.1
    CDS127..5136
    Translation

    Target ORF information:

    RefSeq Version NM_001162399.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen type IV alpha 1 chain (COL4A1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001162399.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTGGCGTT GGCTGGGCTT CGGGCTGCTG CTGAGCGCCG AGCTCAGCGC CGTGTCCGCC 
    GCGGGTTGTT CTGGTTGTGG AAAATGTGAC TGCAGTGGAG TAAAAGGACA AAAGGGAGAA
    AGAGGCCTTC CGGGACTACA GGGTGTGATT GGGTTTCCTG GAATGCAAGG ACCTGAAGGT
    CCCCCTGGGC CACCAGGACT TAAGGGTGAT ACTGGAGAAC CAGGGCTGCC AGGAACAAAA
    GGAACAAGAG GCCCACCAGG ACCATCAGGA TTTCCAGGAA ACCCAGGCCT CCCTGGCATT
    CCTGGACAAG ATGGCCCTCC TGGTCCACCT GGCATTCCAG GATGTAATGG CACAAAGGGT
    GAAAGAGGTC CAGTAGGTCC CCCAGGTTTA CCGGGACTGA CTGGAAGCAT AGGACCCCCA
    GGACCTCCTG GAATTAAGGG AGATCCAGGT GAAGTGATTG GTATTTTACC TCCTAGTGTG
    CTAAAAGGTG ATAAAGGCTT TCCTGGCCAA CCTGGACTAC CTGGTCCACC TGGAACTCCA
    GGGTTTCAGG GACCAATGGG GCCACCAGGG ATTCCAGGAG AACCAGGTCC CCCCGGGCCA
    CCAGGACCCC CAGGCGAGAA GGGCTACATG GGCTTGGGCT TTCAAGGTCC CAAAGGTGAA
    AAGGGAGAAC AAGGGGTCCA GGGTCCCCCG GGGCCACCAG GACCAGCACC ACACATGAAA
    GAAAAAGGAA GCGAAATCAT AAAGGGAGAA AAGGGTGAGC CAGGTGAAAA GGGTGAACAG
    GGAGTCCCAG GATTGCCAGG CTCAGGTTAC AGAGGAGAAA AGGGTGAACC TGGGAAGCCC
    GGTCCACGTG GAAAGCCTGG AAAAGATGGT GAACCAGGAC CAAAAGGAGA ACCCGGCTTG
    CCTGGAGGGT TTGGGATTCC AGGAAGGCCT GGGGAACCTG GTGCTAAGGG TGAAAAAGGT
    GATCAAGGTC CTCAAGGGCC TCCAGGAAGA ATTATAGATG CTGGACCAAT AGATACTTTT
    GGTGAAAAAG GAGACCCTGG AGTTTCAGGT TTGCCTGGAC TAAAAGGTCA AAAAGGTGAA
    AAAGGTTTCC CTGGTGCACA AGGGATCCCA GGAATTCCAG GTCGACCAGT TCCAGACATG
    GTAGGATCAC CTGGTGTCCC TGGCCAGAGA GGTGAAAAAG GTGAAAAGGG TTCTCCAGGA
    TTCCCTATAC CTGGACCTCC TGGAAGAGAT GGTTTCCCAG GCCCACCCGG GTTGCCCGGC
    CCACCAGGTC CTCCAGGTGT AACAGATGGA ATAGATCAAT GTCAGCAAGG GCAACCTGGT
    GCACCAGGTG TACCTGGACC TCCAGGGCAA CATGGATTTC CAGGAGAGAT GGGAGAGAAA
    GGTGAAAAAG GTGAAACCTG TGCCTTGTGT GATAAAGTAG GACCCCCTGG ACTTCCAGGA
    CCACAAGGAC TACCGGGTCC AGCAGGTTTT CCGGGACAGT CAGGTTTCAA GGGTGACAGA
    GGCTTACCTG GACTTGATGG CCTTCCAGGC GTACCCGGGC CCCCAGGCGC TCCAGGACTC
    ATTGGCCTCC CTGGGGCAAA AGGAGAACCA GGAGACTTTA CTTATGATTC CATCCTGAAA
    GGTGAGAAGG GAGATCCTGG TTTCCCAGGA CAACCAGGTA TTCCTGGAAG AGAAGGAACA
    CCAGGAAAAG ACGGATTGCC AGGTCCTCAA GGTCCAAAAG GAGCATCGGG TACAGCTGGC
    TTGAAAGGAG AACGTGGTCC CCCTGGACAA CCTGGATTCC CTGGAGTACG TGGTGAAAGA
    GGTTTACCTG GCTCTCCTGG GGTAGGCACT GCAGGGTTAC CTGGTGACAA AGGAGAGAGA
    GGTTTTGCTG GAACTCCAGG TTTACCAGGT CTTCCAGGAA CAAAAGGTGA AGCAGGACGA
    ACTGTACCAC TCCCTGGACC TCCTGGGGCA GATGGCCTTC CTGGACCACC TGGTTTCCCT
    GGACCCCAAG GTGACAAAGG GAATCCTGGG CTTCCGGGGA GACCTGGCCT GCCAGGAGAA
    AAAGGTGCTG TTGGGCAGCC AGGTATAGGG TTTCCTGGAC CTCCAGGACC CAAAGGTTTC
    CCAGGTCAGC CTGGCTCACC TGGTCCTCCG GGTACTCCTG GAACCCCTGG CCTGGATGGT
    TTTCCAGGCA CACCAGGTTT ACAAGGTCAA AAAGGTGAAC CTGGAGTGGG TCTCCCTGGT
    CCTAAAGGGT TACCAGGCAT CCCAGGTCCA GCTGGCATCC CTGGAGAAAA AGGAAATCCA
    GGGCTGCCTG GCTTACGAGG TGAACAAGGA TTCCCAGGTG TACCTGGACA ACAAGGATTT
    AGAGGAGATC CAGGCCTCCC TGGACTACAG GGCTTGCCAG GCCCCCAGGG CTCACCAGGA
    GTGGGCCCAC CGGGATTGCC AGGGCCCGCT GGGCAGCCAG GACCTGAGGG GCCACCAGGA
    TTTCCTGGAA TAAAAGGAGA AAGGGGCTTC CCTGGTATCC CAGGTCTAGA TATGCCAGGG
    CCAAAAGGAG ATAAAGGTAG CCAGGGCCAG CCTGGAGTAC CAGGGTCTGT GGGGCTACCT
    GGCCTGCCAG GTCCACAAGG ATCTGTTGGA CTGAAAGGAT CAGCAGGAGC CAAAGGAGAA
    ATGGGAGTTA TGGGAACTCC AGGGTTGCCA GGGACTCCTG GACCAGTTGG AGATCGAGGG
    TTGCCTGGTG AAAAAGGTAG CCAAGGTTTG AAAGGTGTGG TTGGACCACA GGGTGAACCA
    GGTGTCAAAG GGGATAAAGG TGAAGCTGGT CTTCCGGGAA TACCTGGAAG CATGGACACC
    ATGGACATGG TTAGTCTCAA AGGCCAAAAG GGAGATCCTG GAGAAAAAGG GGACCTTGGT
    CCAACAGGAG AAAAAGGGTT ACGTGGGGAG TATGGAGAAC CAGGAATTCC AGGAAGGGAT
    GGTGAACCTG GTACTCCGGG ACAACCAGGA CCAAAAGGAG ATCAAGGCCC CCCAGGGTAC
    CCAGGAGATC CAGGAATTCC AGGGCAGAAA GGATCAGTTG GTGAAATGGG TTTGCCAGGA
    ACACCTGGAG AAAAGGGTCT GCCTGGAATA CCAGGTTCAC CAGGCACACC AGGGTTCCCT
    GGGCAAAAAG GTGAAAAAGG AGACAAAGGA GCAGCAGGTT TTCCTGGAAT TGGCTTTCCA
    GGATCTCCTG GTGAGAAGGG AGAACCAGGA AGAACAGGCA GTCCGGGTTT GTCTGGAGAC
    AAAGGTGAAA AGGGTAATGC AGGAATTCCA GGAATGCCGG GTGCTCCAGG TCCCAAAGGA
    TCCCCTGGCA TTGCAGGATT TCCAGGCAAC CCAGGCCTCC ATGGAGCAAA GGGTGAAAAA
    GGACTTCCTG GCTTAAGTGG CATCCCAGGC TTAAAAGGAG AACCAGGTGA ACCTGGTGTT
    CCTGGTCCTG CTGGTTCCAT TGGTCAGAAG GGTGAACCAG GCTATGATGG GATTCCAGGC
    ACAGCTGGTG CAAAGGGTGA ACAAGGTGTA CCAGGTAGAG GAGTTCCAGG ATATCCAGGT
    GCAAAGGGAG ATAAAGGTGC AAAGGGTGAA GTGGGTTTCC CAGGATCCCC AGGAATTCCA
    GGGATTCCAG GCTTAAAAGG AGAACAAGGG TATGCTGGCC CACCCGGCCC TAAAGGCAAC
    CAAGGTCTTC CTGGACTACC AGGAAGTGCA ATTGAGGGCC CTAAAGGAGA CAGAGGACCA
    CAAGGCCAAC CCGGCCTTCC AGGTTTACCA GGTCCAACGG GGCCACCAGG ACCTCCAGGT
    CTGAAGGGAG CTAAAGGAGA GCAGGGAAGC GATGGCTGGC CTGGAACTCC TGGAGGTCCA
    GGAGATAAAG GCGATCCAGG TTTCCAGGGA CTGCCAGGTA GTCCTGGTTT GCCAGGAGGT
    GCTGGTGAAA AGGGTGATCT AGGGCCTCCA GGTGTTCCAG GTGTTCCAGG TCCAAAAGGA
    TCTGCAGGCT TCCCAGGCCT CAAGGGTGAG CGAGGTGACC AAGGTCTTCC TGGATCTAAA
    GGTTTACAAG GCCCGCCGGG CCCCCCAGGT GCTTTTCAGC TTATCAAAGG AGATCCTGGC
    TTACCTGGCC CGGTAGGACC AGTTGGTCTC AAAGGATTCC CAGGACATCC AGGTCCCAAA
    GGACAGCAAG GAGTAACAGG GCCTTCAGGT GTACCTGGAC CACCTGGTAG TCCAGGGTTT
    GATGGCAGAC CAGGGCAAAA AGGAGAAGTT GGTCCTTATG GTCCTCCAGG GCCCAGAGGA
    ATCCCTGGTC CGCCTGGCCC TGAAGGTTTG CCTGGGGCTA TGGGTCCACC AGGTGCAGCA
    TCTGTTGCTC ATGGCTTCCT TGTCACCAGA CACAGTCAAA CCACTGAAGA ACCACAATGT
    CCATTTGGAA CAAGGCTGAT TTACCATGGC TACTCCCTTC TTTATGTACA AGGAAATGAA
    AGAGCACACG GTCAAGATCT GGGCACTGCA GGGAGCTGTC TTCGTAAATT TAGTACTATG
    CCTTTCTTAT TTTGCAATAT CAACAATGTA TGTAACTTTG CATCAAGGAA TGACTATTCC
    TACTGGCTGT CCACTCCTGA ACCCATGCCA ATGAGTATGG CACCTATCAC TGGTGAAAGT
    ATCAGACCAT TCATCAGCAG ATGCTCTGTG TGTGAAGCTC CTGCTATGGT AATCGCCGTT
    CACAGTCAAA CAATCCAGAT ACCACCATGT CCTGAAGGCT GGAGTTCCCT TTGGATTGGT
    TACTCCTTTG TCATGCACAC CAGTGCTGGT GCTGAGGGCT CTGGCCAGGC CCTGGCGTCT
    CCTGGTTCCT GCCTGGAGGA GTTCCGCAGC GCTCCCTTCA TTGAATGCCA TGGCCGGGGC
    ACCTGCAATT ATTATGCAAA TGCTTACAGC TTCTGGCTTG CCACTATAGA GAGGAATGAG
    ATGTTCAAGA AACCGACTCC GTCAACTTTG AAAGCAGGGG ATCTGCGCTC CAACGTTAGC
    CGTTGTCAAG TCTGTATGAG AAATACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30657
    Clone ID Related Accession (Same CDS sequence) XM_015277823.2 , XM_015277823.1
    Accession Version XM_015277823.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5010bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product collagen alpha-1(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277823.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTGGCGTT GGCTGGGCTT CGGGCTGCTG CTGAGCGCCG AGCTCAGCGC CGTGTCCGCC 
    GCGGGTTGTT CTGGTTGTGG AAAATGTGAC TGCAGTGGAG TAAAAGGACA AAAGGGAGAA
    AGAGGCCTTC CGGGACTACA GGGTGTGATT GGGTTTCCTG GAATGCAAGG ACCTGAAGGT
    CCCCCTGGGC CACCAGGACT TAAGGGTGAT ACTGGAGAAC CAGGGCTGCC AGGAACAAAA
    GGAACAAGAG GCCCACCAGG ACCATCAGGA TTTCCAGGAA ACCCAGGCCT CCCTGGCATT
    CCTGGACAAG ATGGCCCTCC TGGTCCACCT GGCATTCCAG GATGTAATGG CACAAAGGGT
    GAAAGAGGTC CAGTAGGTCC CCCAGGTTTA CCGGGACTGA CTGGAAGCAT AGGACCCCCA
    GGACCTCCTG GAATTAAGGG AGATCCAGGT GAAGTGATTG GTATTTTACC TCCTAGTGTG
    CTAAAAGGTG ATAAAGGCTT TCCTGGCCAA CCTGGACTAC CTGGTCCACC TGGAACTCCA
    GGGTTTCAGG GACCAATGGG GCCACCAGGG ATTCCAGGAG AACCAGGTCC CCCCGGGCCA
    CCAGGACCCC CAGGCGAGAA GGGCTACATG GGCTTGGGCT TTCAAGGTCC CAAAGGTGAA
    AAGGGAGAAC AAGGGGTCCA GGGTCCCCCG GGGCCACCAG GACCAGCACC ACACATGAAA
    GAAAAAGGAA GCGAAATCAT AAAGGGAGAA AAGGGTGAGC CAGGTGAAAA GGGTGAACAG
    GGAGTCCCAG GATTGCCAGG CTCAGGTTAC AGAGGAGAAA AGGGTGAACC TGGGAAGCCC
    GGTCCACGTG GAAAGCCTGG AAAAGATGGT GAACCAGGAC CAAAAGGAGA ACCCGGCTTG
    CCTGGAGGGT TTGGGATTCC AGGAAGGCCT GGGGAACCTG GTGCTAAGGG TGAAAAAGGT
    GATCAAGGTC CTCAAGGGCC TCCAGGAAGA ATTATAGATG CTGGACCAAT AGATACTTTT
    GGTGAAAAAG GAGACCCTGG AGTTTCAGGT TTGCCTGGAC TAAAAGGTCA AAAAGGTGAA
    AAAGGTTTCC CTGGTGCACA AGGGATCCCA GGAATTCCAG GTCGACCAGT TCCAGACATG
    GTAGGATCAC CTGGTGTCCC TGGCCAGAGA GGTGAAAAAG GTGAAAAGGG TTCTCCAGGA
    TTCCCTATAC CTGGACCTCC TGGAAGAGAT GGTTTCCCAG GCCCACCCGG GTTGCCCGGC
    CCACCAGGTC CTCCAGGTGT AACAGATGGA ATAGATCAAT GTCAGCAAGG GCAACCTGGT
    GCACCAGGTG TACCTGGACC TCCAGGGCAA CATGGATTTC CAGGAGAGAT GGGAGAGAAA
    GGTGAAAAAG GTGAAACCTG TGCCTTGTGT GATAAAGTAG GACCCCCTGG ACTTCCAGGA
    CCACAAGGAC TACCGGGTCC AGCAGGTTTT CCGGGACAGT CAGGTTTCAA GGGTGACAGA
    GGCTTACCTG GACTTGATGG CCTTCCAGGC GTACCCGGGC CCCCAGGCGC TCCAGGACTC
    ATTGGCCTCC CTGGGGCAAA AGGAGAACCA GGAGACTTTA CTTATGATTC CATCCTGAAA
    GGTGAGAAGG GAGATCCTGG TTTCCCAGGA CAACCAGGTA TTCCTGGAAG AGAAGGAACA
    CCAGGAAAAG ACGGATTGCC AGGTCCTCAA GGTCCAAAAG GAGCATCGGG TACAGCTGGC
    TTGAAAGGAG AACGTGGTCC CCCTGGACAA CCTGGATTCC CTGGAGTACG TGGTGAAAGA
    GGTTTACCTG GCTCTCCTGG GGTAGGCACT GCAGGGTTAC CTGGTGACAA AGGAGAGAGA
    GGTTTTGCTG GAACTCCAGG TTTACCAGGT CTTCCAGGAA CAAAAGGTGA AGCAGGACGA
    ACTGTACCAC TCCCTGGACC TCCTGGGGCA GATGGCCTTC CTGGACCACC TGGTTTCCCT
    GGACCCCAAG GTGACAAAGG GAATCCTGGG CTTCCGGGGA GACCTGGCCT GCCAGGAGAA
    AAAGGTGCTG TTGGGCAGCC AGGTATAGGG TTTCCTGGAC CTCCAGGACC CAAAGGTTTC
    CCAGGTCAGC CTGGCTCACC TGGTCCTCCG GGTACTCCTG GAACCCCTGG CCTGGATGGT
    TTTCCAGGCA CACCAGGTTT ACAAGGTCAA AAAGGTGAAC CTGGAGTGGG TCTCCCTGGT
    CCTAAAGGGT TACCAGGCAT CCCAGGTCCA GCTGGCATCC CTGGAGAAAA AGGAAATCCA
    GGGCTGCCTG GCTTACGAGG TGAACAAGGA TTCCCAGGTG TACCTGGACA ACAAGGATTT
    AGAGGAGATC CAGGCCTCCC TGGACTACAG GGCTTGCCAG GCCCCCAGGG CTCACCAGGA
    GTGGGCCCAC CGGGATTGCC AGGGCCCGCT GGGCAGCCAG GACCTGAGGG GCCACCAGGA
    TTTCCTGGAA TAAAAGGAGA AAGGGGCTTC CCTGGTATCC CAGGTCTAGA TATGCCAGGG
    CCAAAAGGAG ATAAAGGTAG CCAGGGCCAG CCTGGAGTAC CAGGGTCTGT GGGGCTACCT
    GGCCTGCCAG GTCCACAAGG ATCTGTTGGA CTGAAAGGAT CAGCAGGAGC CAAAGGAGAA
    ATGGGAGTTA TGGGAACTCC AGGGTTGCCA GGGACTCCTG GACCAGTTGG AGATCGAGGG
    TTGCCTGGTG AAAAAGGTAG CCAAGGTTTG AAAGGTGTGG TTGGACCACA GGGTGAACCA
    GGTGTCAAAG GGGATAAAGG TGAAGCTGGT CTTCCGGGAA TACCTGGAAG CATGGACACC
    ATGGACATGG TTAGTCTCAA AGGCCAAAAG GGAGATCCTG GAGAAAAAGG GGACCTTGGT
    CCAACAGGAG AAAAAGGGTT ACGTGGGGAG TATGGAGAAC CAGGAATTCC AGGAAGGGAT
    GGTGAACCTG GTACTCCGGG ACAACCAGGA CCAAAAGGAG ATCAAGGCCC CCCAGGGTAC
    CCAGGAGATC CAGGAATTCC AGGGCAGAAA GGATCAGTTG GTGAAATGGG TTTGCCAGGA
    ACACCTGGAG AAAAGGGTCT GCCTGGAATA CCAGGTTCAC CAGGCACACC AGGGTTCCCT
    GGGCAAAAAG GTGAAAAAGG AGACAAAGGA GCAGCAGGTT TTCCTGGAAT TGGCTTTCCA
    GGATCTCCTG GTGAGAAGGG AGAACCAGGA AGAACAGGCA GTCCGGGTTT GTCTGGAGAC
    AAAGGTGAAA AGGGTAATGC AGGAATTCCA GGAATGCCGG GTGCTCCAGG TCCCAAAGGA
    TCCCCTGGCA TTGCAGGATT TCCAGGGAGC CCAGGCCTCC ATGGAGCAAA GGGTGAAAAA
    GGACTTCCTG GCTTAAGTGG CATCCCAGGC TTAAAAGGAG AACCAGGTGA ACCTGGTGTT
    CCTGGTCCTG CTGGTTCCAT TGGTCAGAAG GGTGAACCAG GCTATGATGG GATTCCAGGC
    ACAGCTGGTG CAAAGGGTGA ACAAGGTGTA CCAGGTAGAG GAGTTCCAGG ATATCCAGGT
    GCAAAGGGAG ATAAAGGTGC AAAGGGTGAA GTGGGTTTCC CAGGATCCCC AGGAATTCCA
    GGGATTCCAG GCTTAAAAGG AGAACAAGGG TATGCTGGCC CACCCGGCCC TAAAGGCAAC
    CAAGGTCTTC CTGGACTACC AGGAAGTGCA ATTGAGGGCC CTAAAGGAGA CAGAGGACCA
    CAAGGCCAAC CCGGCCTTCC AGGTTTACCA GGTCCAACGG GGCCACCAGG ACCTCCAGGT
    CTGAAGGGAG CTAAAGGAGA GCAGGGAAGC GATGGCTGGC CTGGAACTCC TGGAGGTCCA
    GGAGATAAAG GCGATCCAGG TTTCCAGGGA CTGCCAGGTA GTCCTGGTTT GCCAGGAGGT
    GCTGGTGAAA AGGGTGATCT AGGGCCTCCA GGTGTTCCAG GTGTTCCAGG TCCAAAAGGA
    TCTGCAGGCT TCCCAGGCCT CAAGGGTGAG CGAGGTGACC AAGGTCTTCC TGGATCTAAA
    GGTTTACAAG GCCCGCCGGG CCCCCCAGGT GCTTTTCAGC TTATCAAAGG AGATCCTGGC
    TTACCTGGCC CGGTAGGACC AGTTGGTCTC AAAGGATTCC CAGGACATCC AGGTCCCAAA
    GGACAGCAAG GAGTAACAGG GCCTTCAGGT GTACCTGGAC CACCTGGTAG TCCAGGGTTT
    GATGGCAGAC CAGGGCAAAA AGGAGAAGTT GGTCCTTATG GTCCTCCAGG GCCCAGAGGA
    ATCCCTGGTC CGCCTGGCCC TGAAGGTTTG CCTGGGGCTA TGGGTCCACC AGGTGCAGCA
    TCTGTTGCTC ATGGCTTCCT TGTCACCAGA CACAGTCAAA CCACTGAAGA ACCACAATGT
    CCATTTGGAA CAAGGCTGAT TTACCATGGC TACTCCCTTC TTTATGTACA AGGAAATGAA
    AGAGCACACG GTCAAGATCT GGGCACTGCA GGGAGCTGTC TTCGTAAATT TAGTACTATG
    CCTTTCTTAT TTTGCAATAT CAACAATGTA TGTAACTTTG CATCAAGGAA TGACTATTCC
    TACTGGCTGT CCACTCCTGA ACCCATGCCA ATGAGTATGG CACCTATCAC TGGTGAAAGT
    ATCAGACCAT TCATCAGCAG ATGCTCTGTG TGTGAAGCTC CTGCTATGGT AATCGCCGTT
    CACAGTCAAA CAATCCAGAT ACCACCATGT CCTGAAGGCT GGAGTTCCCT TTGGATTGGT
    TACTCCTTTG TCATGCACAC CAGTGCTGGT GCTGAGGGCT CTGGCCAGGC CCTGGCGTCT
    CCTGGTTCCT GCCTGGAGGA GTTCCGCAGC GCTCCCTTCA TTGAATGCCA TGGCCGGGGC
    ACCTGCAATT ATTATGCAAA TGCTTACAGC TTCTGGCTTG CCACTATAGA GAGGAATGAG
    ATGTTCAAGA AACCGACTCC GTCAACTTTG AAAGCAGGGG ATCTGCGCTC CAACGTTAGC
    CGTTGTCAAG TCTGTATGAG AAATACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133309.1
    CDS141..5150
    Translation

    Target ORF information:

    RefSeq Version XM_015277823.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen type IV alpha 1 chain (COL4A1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277823.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTGGCGTT GGCTGGGCTT CGGGCTGCTG CTGAGCGCCG AGCTCAGCGC CGTGTCCGCC 
    GCGGGTTGTT CTGGTTGTGG AAAATGTGAC TGCAGTGGAG TAAAAGGACA AAAGGGAGAA
    AGAGGCCTTC CGGGACTACA GGGTGTGATT GGGTTTCCTG GAATGCAAGG ACCTGAAGGT
    CCCCCTGGGC CACCAGGACT TAAGGGTGAT ACTGGAGAAC CAGGGCTGCC AGGAACAAAA
    GGAACAAGAG GCCCACCAGG ACCATCAGGA TTTCCAGGAA ACCCAGGCCT CCCTGGCATT
    CCTGGACAAG ATGGCCCTCC TGGTCCACCT GGCATTCCAG GATGTAATGG CACAAAGGGT
    GAAAGAGGTC CAGTAGGTCC CCCAGGTTTA CCGGGACTGA CTGGAAGCAT AGGACCCCCA
    GGACCTCCTG GAATTAAGGG AGATCCAGGT GAAGTGATTG GTATTTTACC TCCTAGTGTG
    CTAAAAGGTG ATAAAGGCTT TCCTGGCCAA CCTGGACTAC CTGGTCCACC TGGAACTCCA
    GGGTTTCAGG GACCAATGGG GCCACCAGGG ATTCCAGGAG AACCAGGTCC CCCCGGGCCA
    CCAGGACCCC CAGGCGAGAA GGGCTACATG GGCTTGGGCT TTCAAGGTCC CAAAGGTGAA
    AAGGGAGAAC AAGGGGTCCA GGGTCCCCCG GGGCCACCAG GACCAGCACC ACACATGAAA
    GAAAAAGGAA GCGAAATCAT AAAGGGAGAA AAGGGTGAGC CAGGTGAAAA GGGTGAACAG
    GGAGTCCCAG GATTGCCAGG CTCAGGTTAC AGAGGAGAAA AGGGTGAACC TGGGAAGCCC
    GGTCCACGTG GAAAGCCTGG AAAAGATGGT GAACCAGGAC CAAAAGGAGA ACCCGGCTTG
    CCTGGAGGGT TTGGGATTCC AGGAAGGCCT GGGGAACCTG GTGCTAAGGG TGAAAAAGGT
    GATCAAGGTC CTCAAGGGCC TCCAGGAAGA ATTATAGATG CTGGACCAAT AGATACTTTT
    GGTGAAAAAG GAGACCCTGG AGTTTCAGGT TTGCCTGGAC TAAAAGGTCA AAAAGGTGAA
    AAAGGTTTCC CTGGTGCACA AGGGATCCCA GGAATTCCAG GTCGACCAGT TCCAGACATG
    GTAGGATCAC CTGGTGTCCC TGGCCAGAGA GGTGAAAAAG GTGAAAAGGG TTCTCCAGGA
    TTCCCTATAC CTGGACCTCC TGGAAGAGAT GGTTTCCCAG GCCCACCCGG GTTGCCCGGC
    CCACCAGGTC CTCCAGGTGT AACAGATGGA ATAGATCAAT GTCAGCAAGG GCAACCTGGT
    GCACCAGGTG TACCTGGACC TCCAGGGCAA CATGGATTTC CAGGAGAGAT GGGAGAGAAA
    GGTGAAAAAG GTGAAACCTG TGCCTTGTGT GATAAAGTAG GACCCCCTGG ACTTCCAGGA
    CCACAAGGAC TACCGGGTCC AGCAGGTTTT CCGGGACAGT CAGGTTTCAA GGGTGACAGA
    GGCTTACCTG GACTTGATGG CCTTCCAGGC GTACCCGGGC CCCCAGGCGC TCCAGGACTC
    ATTGGCCTCC CTGGGGCAAA AGGAGAACCA GGAGACTTTA CTTATGATTC CATCCTGAAA
    GGTGAGAAGG GAGATCCTGG TTTCCCAGGA CAACCAGGTA TTCCTGGAAG AGAAGGAACA
    CCAGGAAAAG ACGGATTGCC AGGTCCTCAA GGTCCAAAAG GAGCATCGGG TACAGCTGGC
    TTGAAAGGAG AACGTGGTCC CCCTGGACAA CCTGGATTCC CTGGAGTACG TGGTGAAAGA
    GGTTTACCTG GCTCTCCTGG GGTAGGCACT GCAGGGTTAC CTGGTGACAA AGGAGAGAGA
    GGTTTTGCTG GAACTCCAGG TTTACCAGGT CTTCCAGGAA CAAAAGGTGA AGCAGGACGA
    ACTGTACCAC TCCCTGGACC TCCTGGGGCA GATGGCCTTC CTGGACCACC TGGTTTCCCT
    GGACCCCAAG GTGACAAAGG GAATCCTGGG CTTCCGGGGA GACCTGGCCT GCCAGGAGAA
    AAAGGTGCTG TTGGGCAGCC AGGTATAGGG TTTCCTGGAC CTCCAGGACC CAAAGGTTTC
    CCAGGTCAGC CTGGCTCACC TGGTCCTCCG GGTACTCCTG GAACCCCTGG CCTGGATGGT
    TTTCCAGGCA CACCAGGTTT ACAAGGTCAA AAAGGTGAAC CTGGAGTGGG TCTCCCTGGT
    CCTAAAGGGT TACCAGGCAT CCCAGGTCCA GCTGGCATCC CTGGAGAAAA AGGAAATCCA
    GGGCTGCCTG GCTTACGAGG TGAACAAGGA TTCCCAGGTG TACCTGGACA ACAAGGATTT
    AGAGGAGATC CAGGCCTCCC TGGACTACAG GGCTTGCCAG GCCCCCAGGG CTCACCAGGA
    GTGGGCCCAC CGGGATTGCC AGGGCCCGCT GGGCAGCCAG GACCTGAGGG GCCACCAGGA
    TTTCCTGGAA TAAAAGGAGA AAGGGGCTTC CCTGGTATCC CAGGTCTAGA TATGCCAGGG
    CCAAAAGGAG ATAAAGGTAG CCAGGGCCAG CCTGGAGTAC CAGGGTCTGT GGGGCTACCT
    GGCCTGCCAG GTCCACAAGG ATCTGTTGGA CTGAAAGGAT CAGCAGGAGC CAAAGGAGAA
    ATGGGAGTTA TGGGAACTCC AGGGTTGCCA GGGACTCCTG GACCAGTTGG AGATCGAGGG
    TTGCCTGGTG AAAAAGGTAG CCAAGGTTTG AAAGGTGTGG TTGGACCACA GGGTGAACCA
    GGTGTCAAAG GGGATAAAGG TGAAGCTGGT CTTCCGGGAA TACCTGGAAG CATGGACACC
    ATGGACATGG TTAGTCTCAA AGGCCAAAAG GGAGATCCTG GAGAAAAAGG GGACCTTGGT
    CCAACAGGAG AAAAAGGGTT ACGTGGGGAG TATGGAGAAC CAGGAATTCC AGGAAGGGAT
    GGTGAACCTG GTACTCCGGG ACAACCAGGA CCAAAAGGAG ATCAAGGCCC CCCAGGGTAC
    CCAGGAGATC CAGGAATTCC AGGGCAGAAA GGATCAGTTG GTGAAATGGG TTTGCCAGGA
    ACACCTGGAG AAAAGGGTCT GCCTGGAATA CCAGGTTCAC CAGGCACACC AGGGTTCCCT
    GGGCAAAAAG GTGAAAAAGG AGACAAAGGA GCAGCAGGTT TTCCTGGAAT TGGCTTTCCA
    GGATCTCCTG GTGAGAAGGG AGAACCAGGA AGAACAGGCA GTCCGGGTTT GTCTGGAGAC
    AAAGGTGAAA AGGGTAATGC AGGAATTCCA GGAATGCCGG GTGCTCCAGG TCCCAAAGGA
    TCCCCTGGCA TTGCAGGATT TCCAGGGAGC CCAGGCCTCC ATGGAGCAAA GGGTGAAAAA
    GGACTTCCTG GCTTAAGTGG CATCCCAGGC TTAAAAGGAG AACCAGGTGA ACCTGGTGTT
    CCTGGTCCTG CTGGTTCCAT TGGTCAGAAG GGTGAACCAG GCTATGATGG GATTCCAGGC
    ACAGCTGGTG CAAAGGGTGA ACAAGGTGTA CCAGGTAGAG GAGTTCCAGG ATATCCAGGT
    GCAAAGGGAG ATAAAGGTGC AAAGGGTGAA GTGGGTTTCC CAGGATCCCC AGGAATTCCA
    GGGATTCCAG GCTTAAAAGG AGAACAAGGG TATGCTGGCC CACCCGGCCC TAAAGGCAAC
    CAAGGTCTTC CTGGACTACC AGGAAGTGCA ATTGAGGGCC CTAAAGGAGA CAGAGGACCA
    CAAGGCCAAC CCGGCCTTCC AGGTTTACCA GGTCCAACGG GGCCACCAGG ACCTCCAGGT
    CTGAAGGGAG CTAAAGGAGA GCAGGGAAGC GATGGCTGGC CTGGAACTCC TGGAGGTCCA
    GGAGATAAAG GCGATCCAGG TTTCCAGGGA CTGCCAGGTA GTCCTGGTTT GCCAGGAGGT
    GCTGGTGAAA AGGGTGATCT AGGGCCTCCA GGTGTTCCAG GTGTTCCAGG TCCAAAAGGA
    TCTGCAGGCT TCCCAGGCCT CAAGGGTGAG CGAGGTGACC AAGGTCTTCC TGGATCTAAA
    GGTTTACAAG GCCCGCCGGG CCCCCCAGGT GCTTTTCAGC TTATCAAAGG AGATCCTGGC
    TTACCTGGCC CGGTAGGACC AGTTGGTCTC AAAGGATTCC CAGGACATCC AGGTCCCAAA
    GGACAGCAAG GAGTAACAGG GCCTTCAGGT GTACCTGGAC CACCTGGTAG TCCAGGGTTT
    GATGGCAGAC CAGGGCAAAA AGGAGAAGTT GGTCCTTATG GTCCTCCAGG GCCCAGAGGA
    ATCCCTGGTC CGCCTGGCCC TGAAGGTTTG CCTGGGGCTA TGGGTCCACC AGGTGCAGCA
    TCTGTTGCTC ATGGCTTCCT TGTCACCAGA CACAGTCAAA CCACTGAAGA ACCACAATGT
    CCATTTGGAA CAAGGCTGAT TTACCATGGC TACTCCCTTC TTTATGTACA AGGAAATGAA
    AGAGCACACG GTCAAGATCT GGGCACTGCA GGGAGCTGTC TTCGTAAATT TAGTACTATG
    CCTTTCTTAT TTTGCAATAT CAACAATGTA TGTAACTTTG CATCAAGGAA TGACTATTCC
    TACTGGCTGT CCACTCCTGA ACCCATGCCA ATGAGTATGG CACCTATCAC TGGTGAAAGT
    ATCAGACCAT TCATCAGCAG ATGCTCTGTG TGTGAAGCTC CTGCTATGGT AATCGCCGTT
    CACAGTCAAA CAATCCAGAT ACCACCATGT CCTGAAGGCT GGAGTTCCCT TTGGATTGGT
    TACTCCTTTG TCATGCACAC CAGTGCTGGT GCTGAGGGCT CTGGCCAGGC CCTGGCGTCT
    CCTGGTTCCT GCCTGGAGGA GTTCCGCAGC GCTCCCTTCA TTGAATGCCA TGGCCGGGGC
    ACCTGCAATT ATTATGCAAA TGCTTACAGC TTCTGGCTTG CCACTATAGA GAGGAATGAG
    ATGTTCAAGA AACCGACTCC GTCAACTTTG AAAGCAGGGG ATCTGCGCTC CAACGTTAGC
    CGTTGTCAAG TCTGTATGAG AAATACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30657
    Clone ID Related Accession (Same CDS sequence) XM_015277823.2 , XM_015277823.1
    Accession Version XM_015277823.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5010bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product collagen alpha-1(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763585.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTGGCGTT GGCTGGGCTT CGGGCTGCTG CTGAGCGCCG AGCTCAGCGC CGTGTCCGCC 
    GCGGGTTGTT CTGGTTGTGG AAAATGTGAC TGCAGTGGAG TAAAAGGACA AAAGGGAGAA
    AGAGGCCTTC CGGGACTACA GGGTGTGATT GGGTTTCCTG GAATGCAAGG ACCTGAAGGT
    CCCCCTGGGC CACCAGGACT TAAGGGTGAT ACTGGAGAAC CAGGGCTGCC AGGAACAAAA
    GGAACAAGAG GCCCACCAGG ACCATCAGGA TTTCCAGGAA ACCCAGGCCT CCCTGGCATT
    CCTGGACAAG ATGGCCCTCC TGGTCCACCT GGCATTCCAG GATGTAATGG CACAAAGGGT
    GAAAGAGGTC CAGTAGGTCC CCCAGGTTTA CCGGGACTGA CTGGAAGCAT AGGACCCCCA
    GGACCTCCTG GAATTAAGGG AGATCCAGGT GAAGTGATTG GTATTTTACC TCCTAGTGTG
    CTAAAAGGTG ATAAAGGCTT TCCTGGCCAA CCTGGACTAC CTGGTCCACC TGGAACTCCA
    GGGTTTCAGG GACCAATGGG GCCACCAGGG ATTCCAGGAG AACCAGGTCC CCCCGGGCCA
    CCAGGACCCC CAGGCGAGAA GGGCTACATG GGCTTGGGCT TTCAAGGTCC CAAAGGTGAA
    AAGGGAGAAC AAGGGGTCCA GGGTCCCCCG GGGCCACCAG GACCAGCACC ACACATGAAA
    GAAAAAGGAA GCGAAATCAT AAAGGGAGAA AAGGGTGAGC CAGGTGAAAA GGGTGAACAG
    GGAGTCCCAG GATTGCCAGG CTCAGGTTAC AGAGGAGAAA AGGGTGAACC TGGGAAGCCC
    GGTCCACGTG GAAAGCCTGG AAAAGATGGT GAACCAGGAC CAAAAGGAGA ACCCGGCTTG
    CCTGGAGGGT TTGGGATTCC AGGAAGGCCT GGGGAACCTG GTGCTAAGGG TGAAAAAGGT
    GATCAAGGTC CTCAAGGGCC TCCAGGAAGA ATTATAGATG CTGGACCAAT AGATACTTTT
    GGTGAAAAAG GAGACCCTGG AGTTTCAGGT TTGCCTGGAC TAAAAGGTCA AAAAGGTGAA
    AAAGGTTTCC CTGGTGCACA AGGGATCCCA GGAATTCCAG GTCGACCAGT TCCAGACATG
    GTAGGATCAC CTGGTGTCCC TGGCCAGAGA GGTGAAAAAG GTGAAAAGGG TTCTCCAGGA
    TTCCCTATAC CTGGACCTCC TGGAAGAGAT GGTTTCCCAG GCCCACCCGG GTTGCCCGGC
    CCACCAGGTC CTCCAGGTGT AACAGATGGA ATAGATCAAT GTCAGCAAGG GCAACCTGGT
    GCACCAGGTG TACCTGGACC TCCAGGGCAA CATGGATTTC CAGGAGAGAT GGGAGAGAAA
    GGTGAAAAAG GTGAAACCTG TGCCTTGTGT GATAAAGTAG GACCCCCTGG ACTTCCAGGA
    CCACAAGGAC TACCGGGTCC AGCAGGTTTT CCGGGACAGT CAGGTTTCAA GGGTGACAGA
    GGCTTACCTG GACTTGATGG CCTTCCAGGC GTACCCGGGC CCCCAGGCGC TCCAGGACTC
    ATTGGCCTCC CTGGGGCAAA AGGAGAACCA GGAGACTTTA CTTATGATTC CATCCTGAAA
    GGTGAGAAGG GAGATCCTGG TTTCCCAGGA CAACCAGGTA TTCCTGGAAG AGAAGGAACA
    CCAGGAAAAG ACGGATTGCC AGGTCCTCAA GGTCCAAAAG GAGCATCGGG TACAGCTGGC
    TTGAAAGGAG AACGTGGTCC CCCTGGACAA CCTGGATTCC CTGGAGTACG TGGTGAAAGA
    GGTTTACCTG GCTCTCCTGG GGTAGGCACT GCAGGGTTAC CTGGTGACAA AGGAGAGAGA
    GGTTTTGCTG GAACTCCAGG TTTACCAGGT CTTCCAGGAA CAAAAGGTGA AGCAGGACGA
    ACTGTACCAC TCCCTGGACC TCCTGGGGCA GATGGCCTTC CTGGACCACC TGGTTTCCCT
    GGACCCCAAG GTGACAAAGG GAATCCTGGG CTTCCGGGGA GACCTGGCCT GCCAGGAGAA
    AAAGGTGCTG TTGGGCAGCC AGGTATAGGG TTTCCTGGAC CTCCAGGACC CAAAGGTTTC
    CCAGGTCAGC CTGGCTCACC TGGTCCTCCG GGTACTCCTG GAACCCCTGG CCTGGATGGT
    TTTCCAGGCA CACCAGGTTT ACAAGGTCAA AAAGGTGAAC CTGGAGTGGG TCTCCCTGGT
    CCTAAAGGGT TACCAGGCAT CCCAGGTCCA GCTGGCATCC CTGGAGAAAA AGGAAATCCA
    GGGCTGCCTG GCTTACGAGG TGAACAAGGA TTCCCAGGTG TACCTGGACA ACAAGGATTT
    AGAGGAGATC CAGGCCTCCC TGGACTACAG GGCTTGCCAG GCCCCCAGGG CTCACCAGGA
    GTGGGCCCAC CGGGATTGCC AGGGCCCGCT GGGCAGCCAG GACCTGAGGG GCCACCAGGA
    TTTCCTGGAA TAAAAGGAGA AAGGGGCTTC CCTGGTATCC CAGGTCTAGA TATGCCAGGG
    CCAAAAGGAG ATAAAGGTAG CCAGGGCCAG CCTGGAGTAC CAGGGTCTGT GGGGCTACCT
    GGCCTGCCAG GTCCACAAGG ATCTGTTGGA CTGAAAGGAT CAGCAGGAGC CAAAGGAGAA
    ATGGGAGTTA TGGGAACTCC AGGGTTGCCA GGGACTCCTG GACCAGTTGG AGATCGAGGG
    TTGCCTGGTG AAAAAGGTAG CCAAGGTTTG AAAGGTGTGG TTGGACCACA GGGTGAACCA
    GGTGTCAAAG GGGATAAAGG TGAAGCTGGT CTTCCGGGAA TACCTGGAAG CATGGACACC
    ATGGACATGG TTAGTCTCAA AGGCCAAAAG GGAGATCCTG GAGAAAAAGG GGACCTTGGT
    CCAACAGGAG AAAAAGGGTT ACGTGGGGAG TATGGAGAAC CAGGAATTCC AGGAAGGGAT
    GGTGAACCTG GTACTCCGGG ACAACCAGGA CCAAAAGGAG ATCAAGGCCC CCCAGGGTAC
    CCAGGAGATC CAGGAATTCC AGGGCAGAAA GGATCAGTTG GTGAAATGGG TTTGCCAGGA
    ACACCTGGAG AAAAGGGTCT GCCTGGAATA CCAGGTTCAC CAGGCACACC AGGGTTCCCT
    GGGCAAAAAG GTGAAAAAGG AGACAAAGGA GCAGCAGGTT TTCCTGGAAT TGGCTTTCCA
    GGATCTCCTG GTGAGAAGGG AGAACCAGGA AGAACAGGCA GTCCGGGTTT GTCTGGAGAC
    AAAGGTGAAA AGGGTAATGC AGGAATTCCA GGAATGCCGG GTGCTCCAGG TCCCAAAGGA
    TCCCCTGGCA TTGCAGGATT TCCAGGGAGC CCAGGCCTCC ATGGAGCAAA GGGTGAAAAA
    GGACTTCCTG GCTTAAGTGG CATCCCAGGC TTAAAAGGAG AACCAGGTGA ACCTGGTGTT
    CCTGGTCCTG CTGGTTCCAT TGGTCAGAAG GGTGAACCAG GCTATGATGG GATTCCAGGC
    ACAGCTGGTG CAAAGGGTGA ACAAGGTGTA CCAGGTAGAG GAGTTCCAGG ATATCCAGGT
    GCAAAGGGAG ATAAAGGTGC AAAGGGTGAA GTGGGTTTCC CAGGATCCCC AGGAATTCCA
    GGGATTCCAG GCTTAAAAGG AGAACAAGGG TATGCTGGCC CACCCGGCCC TAAAGGCAAC
    CAAGGTCTTC CTGGACTACC AGGAAGTGCA ATTGAGGGCC CTAAAGGAGA CAGAGGACCA
    CAAGGCCAAC CCGGCCTTCC AGGTTTACCA GGTCCAACGG GGCCACCAGG ACCTCCAGGT
    CTGAAGGGAG CTAAAGGAGA GCAGGGAAGC GATGGCTGGC CTGGAACTCC TGGAGGTCCA
    GGAGATAAAG GCGATCCAGG TTTCCAGGGA CTGCCAGGTA GTCCTGGTTT GCCAGGAGGT
    GCTGGTGAAA AGGGTGATCT AGGGCCTCCA GGTGTTCCAG GTGTTCCAGG TCCAAAAGGA
    TCTGCAGGCT TCCCAGGCCT CAAGGGTGAG CGAGGTGACC AAGGTCTTCC TGGATCTAAA
    GGTTTACAAG GCCCGCCGGG CCCCCCAGGT GCTTTTCAGC TTATCAAAGG AGATCCTGGC
    TTACCTGGCC CGGTAGGACC AGTTGGTCTC AAAGGATTCC CAGGACATCC AGGTCCCAAA
    GGACAGCAAG GAGTAACAGG GCCTTCAGGT GTACCTGGAC CACCTGGTAG TCCAGGGTTT
    GATGGCAGAC CAGGGCAAAA AGGAGAAGTT GGTCCTTATG GTCCTCCAGG GCCCAGAGGA
    ATCCCTGGTC CGCCTGGCCC TGAAGGTTTG CCTGGGGCTA TGGGTCCACC AGGTGCAGCA
    TCTGTTGCTC ATGGCTTCCT TGTCACCAGA CACAGTCAAA CCACTGAAGA ACCACAATGT
    CCATTTGGAA CAAGGCTGAT TTACCATGGC TACTCCCTTC TTTATGTACA AGGAAATGAA
    AGAGCACACG GTCAAGATCT GGGCACTGCA GGGAGCTGTC TTCGTAAATT TAGTACTATG
    CCTTTCTTAT TTTGCAATAT CAACAATGTA TGTAACTTTG CATCAAGGAA TGACTATTCC
    TACTGGCTGT CCACTCCTGA ACCCATGCCA ATGAGTATGG CACCTATCAC TGGTGAAAGT
    ATCAGACCAT TCATCAGCAG ATGCTCTGTG TGTGAAGCTC CTGCTATGGT AATCGCCGTT
    CACAGTCAAA CAATCCAGAT ACCACCATGT CCTGAAGGCT GGAGTTCCCT TTGGATTGGT
    TACTCCTTTG TCATGCACAC CAGTGCTGGT GCTGAGGGCT CTGGCCAGGC CCTGGCGTCT
    CCTGGTTCCT GCCTGGAGGA GTTCCGCAGC GCTCCCTTCA TTGAATGCCA TGGCCGGGGC
    ACCTGCAATT ATTATGCAAA TGCTTACAGC TTCTGGCTTG CCACTATAGA GAGGAATGAG
    ATGTTCAAGA AACCGACTCC GTCAACTTTG AAAGCAGGGG ATCTGCGCTC CAACGTTAGC
    CGTTGTCAAG TCTGTATGAG AAATACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133309.1
    CDS120..5129
    Translation

    Target ORF information:

    RefSeq Version XM_015277823.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen, type IV, alpha 1 (COL4A1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277823.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTGGCGTT GGCTGGGCTT CGGGCTGCTG CTGAGCGCCG AGCTCAGCGC CGTGTCCGCC 
    GCGGGTTGTT CTGGTTGTGG AAAATGTGAC TGCAGTGGAG TAAAAGGACA AAAGGGAGAA
    AGAGGCCTTC CGGGACTACA GGGTGTGATT GGGTTTCCTG GAATGCAAGG ACCTGAAGGT
    CCCCCTGGGC CACCAGGACT TAAGGGTGAT ACTGGAGAAC CAGGGCTGCC AGGAACAAAA
    GGAACAAGAG GCCCACCAGG ACCATCAGGA TTTCCAGGAA ACCCAGGCCT CCCTGGCATT
    CCTGGACAAG ATGGCCCTCC TGGTCCACCT GGCATTCCAG GATGTAATGG CACAAAGGGT
    GAAAGAGGTC CAGTAGGTCC CCCAGGTTTA CCGGGACTGA CTGGAAGCAT AGGACCCCCA
    GGACCTCCTG GAATTAAGGG AGATCCAGGT GAAGTGATTG GTATTTTACC TCCTAGTGTG
    CTAAAAGGTG ATAAAGGCTT TCCTGGCCAA CCTGGACTAC CTGGTCCACC TGGAACTCCA
    GGGTTTCAGG GACCAATGGG GCCACCAGGG ATTCCAGGAG AACCAGGTCC CCCCGGGCCA
    CCAGGACCCC CAGGCGAGAA GGGCTACATG GGCTTGGGCT TTCAAGGTCC CAAAGGTGAA
    AAGGGAGAAC AAGGGGTCCA GGGTCCCCCG GGGCCACCAG GACCAGCACC ACACATGAAA
    GAAAAAGGAA GCGAAATCAT AAAGGGAGAA AAGGGTGAGC CAGGTGAAAA GGGTGAACAG
    GGAGTCCCAG GATTGCCAGG CTCAGGTTAC AGAGGAGAAA AGGGTGAACC TGGGAAGCCC
    GGTCCACGTG GAAAGCCTGG AAAAGATGGT GAACCAGGAC CAAAAGGAGA ACCCGGCTTG
    CCTGGAGGGT TTGGGATTCC AGGAAGGCCT GGGGAACCTG GTGCTAAGGG TGAAAAAGGT
    GATCAAGGTC CTCAAGGGCC TCCAGGAAGA ATTATAGATG CTGGACCAAT AGATACTTTT
    GGTGAAAAAG GAGACCCTGG AGTTTCAGGT TTGCCTGGAC TAAAAGGTCA AAAAGGTGAA
    AAAGGTTTCC CTGGTGCACA AGGGATCCCA GGAATTCCAG GTCGACCAGT TCCAGACATG
    GTAGGATCAC CTGGTGTCCC TGGCCAGAGA GGTGAAAAAG GTGAAAAGGG TTCTCCAGGA
    TTCCCTATAC CTGGACCTCC TGGAAGAGAT GGTTTCCCAG GCCCACCCGG GTTGCCCGGC
    CCACCAGGTC CTCCAGGTGT AACAGATGGA ATAGATCAAT GTCAGCAAGG GCAACCTGGT
    GCACCAGGTG TACCTGGACC TCCAGGGCAA CATGGATTTC CAGGAGAGAT GGGAGAGAAA
    GGTGAAAAAG GTGAAACCTG TGCCTTGTGT GATAAAGTAG GACCCCCTGG ACTTCCAGGA
    CCACAAGGAC TACCGGGTCC AGCAGGTTTT CCGGGACAGT CAGGTTTCAA GGGTGACAGA
    GGCTTACCTG GACTTGATGG CCTTCCAGGC GTACCCGGGC CCCCAGGCGC TCCAGGACTC
    ATTGGCCTCC CTGGGGCAAA AGGAGAACCA GGAGACTTTA CTTATGATTC CATCCTGAAA
    GGTGAGAAGG GAGATCCTGG TTTCCCAGGA CAACCAGGTA TTCCTGGAAG AGAAGGAACA
    CCAGGAAAAG ACGGATTGCC AGGTCCTCAA GGTCCAAAAG GAGCATCGGG TACAGCTGGC
    TTGAAAGGAG AACGTGGTCC CCCTGGACAA CCTGGATTCC CTGGAGTACG TGGTGAAAGA
    GGTTTACCTG GCTCTCCTGG GGTAGGCACT GCAGGGTTAC CTGGTGACAA AGGAGAGAGA
    GGTTTTGCTG GAACTCCAGG TTTACCAGGT CTTCCAGGAA CAAAAGGTGA AGCAGGACGA
    ACTGTACCAC TCCCTGGACC TCCTGGGGCA GATGGCCTTC CTGGACCACC TGGTTTCCCT
    GGACCCCAAG GTGACAAAGG GAATCCTGGG CTTCCGGGGA GACCTGGCCT GCCAGGAGAA
    AAAGGTGCTG TTGGGCAGCC AGGTATAGGG TTTCCTGGAC CTCCAGGACC CAAAGGTTTC
    CCAGGTCAGC CTGGCTCACC TGGTCCTCCG GGTACTCCTG GAACCCCTGG CCTGGATGGT
    TTTCCAGGCA CACCAGGTTT ACAAGGTCAA AAAGGTGAAC CTGGAGTGGG TCTCCCTGGT
    CCTAAAGGGT TACCAGGCAT CCCAGGTCCA GCTGGCATCC CTGGAGAAAA AGGAAATCCA
    GGGCTGCCTG GCTTACGAGG TGAACAAGGA TTCCCAGGTG TACCTGGACA ACAAGGATTT
    AGAGGAGATC CAGGCCTCCC TGGACTACAG GGCTTGCCAG GCCCCCAGGG CTCACCAGGA
    GTGGGCCCAC CGGGATTGCC AGGGCCCGCT GGGCAGCCAG GACCTGAGGG GCCACCAGGA
    TTTCCTGGAA TAAAAGGAGA AAGGGGCTTC CCTGGTATCC CAGGTCTAGA TATGCCAGGG
    CCAAAAGGAG ATAAAGGTAG CCAGGGCCAG CCTGGAGTAC CAGGGTCTGT GGGGCTACCT
    GGCCTGCCAG GTCCACAAGG ATCTGTTGGA CTGAAAGGAT CAGCAGGAGC CAAAGGAGAA
    ATGGGAGTTA TGGGAACTCC AGGGTTGCCA GGGACTCCTG GACCAGTTGG AGATCGAGGG
    TTGCCTGGTG AAAAAGGTAG CCAAGGTTTG AAAGGTGTGG TTGGACCACA GGGTGAACCA
    GGTGTCAAAG GGGATAAAGG TGAAGCTGGT CTTCCGGGAA TACCTGGAAG CATGGACACC
    ATGGACATGG TTAGTCTCAA AGGCCAAAAG GGAGATCCTG GAGAAAAAGG GGACCTTGGT
    CCAACAGGAG AAAAAGGGTT ACGTGGGGAG TATGGAGAAC CAGGAATTCC AGGAAGGGAT
    GGTGAACCTG GTACTCCGGG ACAACCAGGA CCAAAAGGAG ATCAAGGCCC CCCAGGGTAC
    CCAGGAGATC CAGGAATTCC AGGGCAGAAA GGATCAGTTG GTGAAATGGG TTTGCCAGGA
    ACACCTGGAG AAAAGGGTCT GCCTGGAATA CCAGGTTCAC CAGGCACACC AGGGTTCCCT
    GGGCAAAAAG GTGAAAAAGG AGACAAAGGA GCAGCAGGTT TTCCTGGAAT TGGCTTTCCA
    GGATCTCCTG GTGAGAAGGG AGAACCAGGA AGAACAGGCA GTCCGGGTTT GTCTGGAGAC
    AAAGGTGAAA AGGGTAATGC AGGAATTCCA GGAATGCCGG GTGCTCCAGG TCCCAAAGGA
    TCCCCTGGCA TTGCAGGATT TCCAGGGAGC CCAGGCCTCC ATGGAGCAAA GGGTGAAAAA
    GGACTTCCTG GCTTAAGTGG CATCCCAGGC TTAAAAGGAG AACCAGGTGA ACCTGGTGTT
    CCTGGTCCTG CTGGTTCCAT TGGTCAGAAG GGTGAACCAG GCTATGATGG GATTCCAGGC
    ACAGCTGGTG CAAAGGGTGA ACAAGGTGTA CCAGGTAGAG GAGTTCCAGG ATATCCAGGT
    GCAAAGGGAG ATAAAGGTGC AAAGGGTGAA GTGGGTTTCC CAGGATCCCC AGGAATTCCA
    GGGATTCCAG GCTTAAAAGG AGAACAAGGG TATGCTGGCC CACCCGGCCC TAAAGGCAAC
    CAAGGTCTTC CTGGACTACC AGGAAGTGCA ATTGAGGGCC CTAAAGGAGA CAGAGGACCA
    CAAGGCCAAC CCGGCCTTCC AGGTTTACCA GGTCCAACGG GGCCACCAGG ACCTCCAGGT
    CTGAAGGGAG CTAAAGGAGA GCAGGGAAGC GATGGCTGGC CTGGAACTCC TGGAGGTCCA
    GGAGATAAAG GCGATCCAGG TTTCCAGGGA CTGCCAGGTA GTCCTGGTTT GCCAGGAGGT
    GCTGGTGAAA AGGGTGATCT AGGGCCTCCA GGTGTTCCAG GTGTTCCAGG TCCAAAAGGA
    TCTGCAGGCT TCCCAGGCCT CAAGGGTGAG CGAGGTGACC AAGGTCTTCC TGGATCTAAA
    GGTTTACAAG GCCCGCCGGG CCCCCCAGGT GCTTTTCAGC TTATCAAAGG AGATCCTGGC
    TTACCTGGCC CGGTAGGACC AGTTGGTCTC AAAGGATTCC CAGGACATCC AGGTCCCAAA
    GGACAGCAAG GAGTAACAGG GCCTTCAGGT GTACCTGGAC CACCTGGTAG TCCAGGGTTT
    GATGGCAGAC CAGGGCAAAA AGGAGAAGTT GGTCCTTATG GTCCTCCAGG GCCCAGAGGA
    ATCCCTGGTC CGCCTGGCCC TGAAGGTTTG CCTGGGGCTA TGGGTCCACC AGGTGCAGCA
    TCTGTTGCTC ATGGCTTCCT TGTCACCAGA CACAGTCAAA CCACTGAAGA ACCACAATGT
    CCATTTGGAA CAAGGCTGAT TTACCATGGC TACTCCCTTC TTTATGTACA AGGAAATGAA
    AGAGCACACG GTCAAGATCT GGGCACTGCA GGGAGCTGTC TTCGTAAATT TAGTACTATG
    CCTTTCTTAT TTTGCAATAT CAACAATGTA TGTAACTTTG CATCAAGGAA TGACTATTCC
    TACTGGCTGT CCACTCCTGA ACCCATGCCA ATGAGTATGG CACCTATCAC TGGTGAAAGT
    ATCAGACCAT TCATCAGCAG ATGCTCTGTG TGTGAAGCTC CTGCTATGGT AATCGCCGTT
    CACAGTCAAA CAATCCAGAT ACCACCATGT CCTGAAGGCT GGAGTTCCCT TTGGATTGGT
    TACTCCTTTG TCATGCACAC CAGTGCTGGT GCTGAGGGCT CTGGCCAGGC CCTGGCGTCT
    CCTGGTTCCT GCCTGGAGGA GTTCCGCAGC GCTCCCTTCA TTGAATGCCA TGGCCGGGGC
    ACCTGCAATT ATTATGCAAA TGCTTACAGC TTCTGGCTTG CCACTATAGA GAGGAATGAG
    ATGTTCAAGA AACCGACTCC GTCAACTTTG AAAGCAGGGG ATCTGCGCTC CAACGTTAGC
    CGTTGTCAAG TCTGTATGAG AAATACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30658
    Clone ID Related Accession (Same CDS sequence) XM_015277824.2 , XM_015277824.1
    Accession Version XM_015277824.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5001bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product collagen alpha-1(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277824.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTGGCGTT GGCTGGGCTT CGGGCTGCTG CTGAGCGCCG AGCTCAGCGC CGTGTCCGCC 
    GCGGGTTGTT CTGGTTGTGG AAAATGTGAC TGCAGTGGAG TAAAAGGACA AAAGGGAGAA
    AGAGGCCTTC CGGGACTACA GGGTGTGATT GGGTTTCCTG GAATGCAAGG ACCTGAAGGT
    CCCCCTGGGC CACCAGGACT TAAGGGTGAT ACTGGAGAAC CAGGGCTGCC AGGAACAAAA
    GGAACAAGAG GCCCACCAGG ACCATCAGGA TTTCCAGGAA ACCCAGGCCT CCCTGGCATT
    CCTGGACAAG ATGGCCCTCC TGGTCCACCT GGCATTCCAG GATGTAATGG CACAAAGGGT
    GAAAGAGGTC CAGTAGGTCC CCCAGGTTTA CCGGGACTGA CTGGAAGCAT AGGACCCCCA
    GGACCTCCTG GAATTAAGGG AGATCCAGGT GAAGTGATTG GTATTTTACC TCCTAGTGTG
    CTAAAAGGTG ATAAAGGCTT TCCTGGCCAA CCTGGACTAC CTGGTCCACC TGGAACTCCA
    GGGTTTCAGG GACCAATGGG GCCACCAGGG ATTCCAGGAG AACCAGGTCC CCCCGGGCCA
    CCAGGACCCC CAGGCGAGAA GGGCTACATG GGCTTGGGCT TTCAAGGTCC CAAAGGTGAA
    AAGGGAGAAC AAGGGGTCCA GGGTCCCCCG GGGCCACCAG GACCAGCACC ACACATGAAA
    GAAAAAGGAA GCGAAATCAT AAAGGGAGAA AAGGGTGAGC CAGGTGAAAA GGGTGAACAG
    GGAGTCCCAG GATTGCCAGG CTCAGGTTAC AGAGGAGAAA AGGGTGAACC TGGGAAGCCC
    GGTCCACGTG GAAAGCCTGG AAAAGATGGT GAACCAGGAC CAAAAGGAGA ACCCGGCTTG
    CCTGGAGGGT TTGGGATTCC AGGAAGGCCT GGGGAACCTG GTGCTAAGGG TGAAAAAGGT
    GATCAAGGTC CTCAAGGGCC TCCAGGAAGA ATTATAGATG CTGGACCAAT AGATACTTTT
    GGTGAAAAAG GAGACCCTGG AGTTTCAGGT TTGCCTGGAC TAAAAGGTCA AAAAGGTGAA
    AAAGGTTTCC CTGGTGCACA AGGGATCCCA GGAATTCCAG GTCGACCAGT TCCAGACATG
    GTAGGATCAC CTGGTGTCCC TGGCCAGAGA GGTGAAAAAG GTGAAAAGGG TTCTCCAGGA
    TTCCCTATAC CTGGACCTCC TGGAAGAGAT GGTTTCCCAG GCCCACCCGG GTTGCCCGGC
    CCACCAGGTC CTCCAGGTGT AACAGATGGA ATAGATCAAT GTCAGCAAGG GCAACCTGGT
    GCACCAGGTG TACCTGGACC TCCAGGGCAA CATGGATTTC CAGGAGAGAT GGGAGAGAAA
    GGTGAAAAAG GTGAAACCTG TGCCTTGTGT GATAAAGTAG GACCCCCTGG ACTTCCAGGA
    CCACAAGGAC TACCGGGTCC AGCAGGTTTT CCGGGACAGT CAGGTTTCAA GGGTGACAGA
    GGCTTACCTG GACTTGATGG CCTTCCAGGC GTACCCGGGC CCCCAGGCGC TCCAGGACTC
    ATTGGCCTCC CTGGGGCAAA AGGAGAACCA GGAGACTTTA CTTATGATTC CATCCTGAAA
    GGTGAGAAGG GAGATCCTGG TTTCCCAGGA CAACCAGGTA TTCCTGGAAG AGAAGGAACA
    CCAGGAAAAG ACGGATTGCC AGGTCCTCAA GGTCCAAAAG GAGCATCGGG TACAGCTGGC
    TTGAAAGGAG AACGTGGTCC CCCTGGACAA CCTGGATTCC CTGGAGTACG TGGTGAAAGA
    GGTTTACCTG GCTCTCCTGG GGTAGGCACT GCAGGGTTAC CTGGTGACAA AGGAGAGAGA
    GGTTTTGCTG GAACTCCAGG TTTACCAGGT CTTCCAGGAA CAAAAGGTGA AGCAGGACGA
    ACTGTACCAC TCCCTGGACC TCCTGGGGCA GATGGCCTTC CTGGACCACC TGGTTTCCCT
    GGACCCCAAG GTGACAAAGG GAATCCTGGG CTTCCGGGGA GACCTGGCCT GCCAGGAGAA
    AAAGGTGCTG TTGGGCAGCC AGGTATAGGG TTTCCTGGAC CTCCAGGACC CAAAGGTTTC
    CCAGGTCAGC CTGGCTCACC TGGTCCTCCG GGTACTCCTG GAACCCCTGG CCTGGATGGT
    TTTCCAGGCA CACCAGGTTT ACAAGGTCAA AAAGGTGAAC CTGGAGTGGG TCTCCCTGGT
    CCTAAAGGGT TACCAGGCAT CCCAGGTCCA GCTGGCATCC CTGGAGAAAA AGGAAATCCA
    GGGCTGCCTG GCTTACGAGG TGAACAAGGA TTCCCAGGTG TACCTGGACA ACAAGGATTT
    AGAGGAGATC CAGGCCTCCC TGGACTACAG GGCTTGCCAG GCCCCCAGGG CTCACCAGGA
    GTGGGCCCAC CGGGATTGCC AGGGCCCGCT GGGCAGCCAG GACCTGAGGG GCCACCAGGA
    TTTCCTGGAA TAAAAGGAGA AAGGGGCTTC CCTGGTATCC CAGGTCTAGA TATGCCAGGG
    CCAAAAGGAG ATAAAGGTAG CCAGGGCCAG CCTGGAGTAC CAGGGTCTGT GGGGCTACCT
    GGCCTGCCAG GTCCACAAGG ATCTGTTGGA CTGAAAGGAT CAGCAGGAGC CAAAGGAGAA
    ATGGGAGTTA TGGGAACTCC AGGGTTGCCA GGGACTCCTG GACCAGTTGG AGATCGAGGG
    TTGCCTGGTG AAAAAGGTAG CCAAGGTTTG AAAGGTGTGG TTGGACCACA GGGTGAACCA
    GGTGTCAAAG GGGATAAAGG TGAAGCTGGT CTTCCGGGAA TACCTGGAAG CATGGACACC
    ATGGACATGG TTAGTCTCAA AGGCCAAAAG GGAGATCCTG GAGAAAAAGG GGACCTTGGT
    CCAACAGGAG AAAAAGGGTT ACGTGGGGAG TATGGAGAAC CAGGAATTCC AGGAAGGGAT
    GGTGAACCTG GTACTCCGGG ACAACCAGGA CCAAAAGGAG ATCAAGGCCC CCCAGGGTAC
    CCAGGAGATC CAGGAATTCC AGGGCAGAAA GGATCAGTTG GTGAAATGGG TTTGCCAGGA
    ACACCTGGAG AAAAGGGTCT GCCTGGAATA CCAGGTTCAC CAGGCACACC AGGGTTCCCT
    GGGCAAAAAG GTGAAAAAGG AGACAAAGGA GCAGCAGGTT TTCCTGGAAT TGGCTTTCCA
    GGATCTCCTG GTGAGAAGGG AGAACCAGGA AGAACAGGCA GTCCGGGTTT GTCTGGAGAC
    AAAGGTGAAA AGGGTAATGC AGGAATTCCA GGAATGCCGG GTGCTCCAGG TCCCAAAGGA
    TCCCCTGGCA TTGCAGGATT TCCAGGGAGC CCAGGCCTCC ATGGAGCAAA GGGTGAAAAA
    GGACTTCCTG GCTTAAGTGG CATCCCAGGC TTAAAAGGAG AACCAGGTGA ACCTGGTGTT
    CCTGGTCCTG CTGGTTCCAT TGGTCAGAAG GGTGAACCAG GCTATGATGG GATTCCAGGC
    ACAGCTGGTG CAAAGGGTGA ACAAGGTGTA CCAGGTAGAG GAGTTCCAGG ATATCCAGGT
    GCAAAGGGAG ATAAAGGTGC AAAGGGTGAA GTGGGTTTCC CAGGATCCCC AGGAATTCCA
    GGGATTCCAG GCTTAAAAGG AGAACAAGGG TATGCTGGCC CACCCGGCCC TAAAGGCAAC
    CAAGGTCTTC CTGGACTACC AGGAAGTGCA ATTGAGGGCC CTAAAGGAGA CAGAGGACCA
    CAAGGCCAAC CCGGCCTTCC AGGTTTACCA GGTCCAACGG GGCCACCAGG ACCTCCAGGT
    CTGAAGGGAG CTAAAGGAGA GCAGGGAAGC GATGGCTGGC CTGGAACTCC TGGAGGTCCA
    GGAGATAAAG GCGATCCAGG TTTCCAGGGA CTGCCAGGTA GTCCTGGTTT GCCAGGAGGT
    GCTGGTGAAA AGGGTGATCT AGGGCCTCCA GGTGTTCCAG GTCCAAAAGG ATCTGCAGGC
    TTCCCAGGCC TCAAGGGTGA GCGAGGTGAC CAAGGTCTTC CTGGATCTAA AGGTTTACAA
    GGCCCGCCGG GCCCCCCAGG TGCTTTTCAG CTTATCAAAG GAGATCCTGG CTTACCTGGC
    CCGGTAGGAC CAGTTGGTCT CAAAGGATTC CCAGGACATC CAGGTCCCAA AGGACAGCAA
    GGAGTAACAG GGCCTTCAGG TGTACCTGGA CCACCTGGTA GTCCAGGGTT TGATGGCAGA
    CCAGGGCAAA AAGGAGAAGT TGGTCCTTAT GGTCCTCCAG GGCCCAGAGG AATCCCTGGT
    CCGCCTGGCC CTGAAGGTTT GCCTGGGGCT ATGGGTCCAC CAGGTGCAGC ATCTGTTGCT
    CATGGCTTCC TTGTCACCAG ACACAGTCAA ACCACTGAAG AACCACAATG TCCATTTGGA
    ACAAGGCTGA TTTACCATGG CTACTCCCTT CTTTATGTAC AAGGAAATGA AAGAGCACAC
    GGTCAAGATC TGGGCACTGC AGGGAGCTGT CTTCGTAAAT TTAGTACTAT GCCTTTCTTA
    TTTTGCAATA TCAACAATGT ATGTAACTTT GCATCAAGGA ATGACTATTC CTACTGGCTG
    TCCACTCCTG AACCCATGCC AATGAGTATG GCACCTATCA CTGGTGAAAG TATCAGACCA
    TTCATCAGCA GATGCTCTGT GTGTGAAGCT CCTGCTATGG TAATCGCCGT TCACAGTCAA
    ACAATCCAGA TACCACCATG TCCTGAAGGC TGGAGTTCCC TTTGGATTGG TTACTCCTTT
    GTCATGCACA CCAGTGCTGG TGCTGAGGGC TCTGGCCAGG CCCTGGCGTC TCCTGGTTCC
    TGCCTGGAGG AGTTCCGCAG CGCTCCCTTC ATTGAATGCC ATGGCCGGGG CACCTGCAAT
    TATTATGCAA ATGCTTACAG CTTCTGGCTT GCCACTATAG AGAGGAATGA GATGTTCAAG
    AAACCGACTC CGTCAACTTT GAAAGCAGGG GATCTGCGCT CCAACGTTAG CCGTTGTCAA
    GTCTGTATGA GAAATACATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133310.1
    CDS141..5141
    Translation

    Target ORF information:

    RefSeq Version XM_015277824.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen type IV alpha 1 chain (COL4A1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277824.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTGGCGTT GGCTGGGCTT CGGGCTGCTG CTGAGCGCCG AGCTCAGCGC CGTGTCCGCC 
    GCGGGTTGTT CTGGTTGTGG AAAATGTGAC TGCAGTGGAG TAAAAGGACA AAAGGGAGAA
    AGAGGCCTTC CGGGACTACA GGGTGTGATT GGGTTTCCTG GAATGCAAGG ACCTGAAGGT
    CCCCCTGGGC CACCAGGACT TAAGGGTGAT ACTGGAGAAC CAGGGCTGCC AGGAACAAAA
    GGAACAAGAG GCCCACCAGG ACCATCAGGA TTTCCAGGAA ACCCAGGCCT CCCTGGCATT
    CCTGGACAAG ATGGCCCTCC TGGTCCACCT GGCATTCCAG GATGTAATGG CACAAAGGGT
    GAAAGAGGTC CAGTAGGTCC CCCAGGTTTA CCGGGACTGA CTGGAAGCAT AGGACCCCCA
    GGACCTCCTG GAATTAAGGG AGATCCAGGT GAAGTGATTG GTATTTTACC TCCTAGTGTG
    CTAAAAGGTG ATAAAGGCTT TCCTGGCCAA CCTGGACTAC CTGGTCCACC TGGAACTCCA
    GGGTTTCAGG GACCAATGGG GCCACCAGGG ATTCCAGGAG AACCAGGTCC CCCCGGGCCA
    CCAGGACCCC CAGGCGAGAA GGGCTACATG GGCTTGGGCT TTCAAGGTCC CAAAGGTGAA
    AAGGGAGAAC AAGGGGTCCA GGGTCCCCCG GGGCCACCAG GACCAGCACC ACACATGAAA
    GAAAAAGGAA GCGAAATCAT AAAGGGAGAA AAGGGTGAGC CAGGTGAAAA GGGTGAACAG
    GGAGTCCCAG GATTGCCAGG CTCAGGTTAC AGAGGAGAAA AGGGTGAACC TGGGAAGCCC
    GGTCCACGTG GAAAGCCTGG AAAAGATGGT GAACCAGGAC CAAAAGGAGA ACCCGGCTTG
    CCTGGAGGGT TTGGGATTCC AGGAAGGCCT GGGGAACCTG GTGCTAAGGG TGAAAAAGGT
    GATCAAGGTC CTCAAGGGCC TCCAGGAAGA ATTATAGATG CTGGACCAAT AGATACTTTT
    GGTGAAAAAG GAGACCCTGG AGTTTCAGGT TTGCCTGGAC TAAAAGGTCA AAAAGGTGAA
    AAAGGTTTCC CTGGTGCACA AGGGATCCCA GGAATTCCAG GTCGACCAGT TCCAGACATG
    GTAGGATCAC CTGGTGTCCC TGGCCAGAGA GGTGAAAAAG GTGAAAAGGG TTCTCCAGGA
    TTCCCTATAC CTGGACCTCC TGGAAGAGAT GGTTTCCCAG GCCCACCCGG GTTGCCCGGC
    CCACCAGGTC CTCCAGGTGT AACAGATGGA ATAGATCAAT GTCAGCAAGG GCAACCTGGT
    GCACCAGGTG TACCTGGACC TCCAGGGCAA CATGGATTTC CAGGAGAGAT GGGAGAGAAA
    GGTGAAAAAG GTGAAACCTG TGCCTTGTGT GATAAAGTAG GACCCCCTGG ACTTCCAGGA
    CCACAAGGAC TACCGGGTCC AGCAGGTTTT CCGGGACAGT CAGGTTTCAA GGGTGACAGA
    GGCTTACCTG GACTTGATGG CCTTCCAGGC GTACCCGGGC CCCCAGGCGC TCCAGGACTC
    ATTGGCCTCC CTGGGGCAAA AGGAGAACCA GGAGACTTTA CTTATGATTC CATCCTGAAA
    GGTGAGAAGG GAGATCCTGG TTTCCCAGGA CAACCAGGTA TTCCTGGAAG AGAAGGAACA
    CCAGGAAAAG ACGGATTGCC AGGTCCTCAA GGTCCAAAAG GAGCATCGGG TACAGCTGGC
    TTGAAAGGAG AACGTGGTCC CCCTGGACAA CCTGGATTCC CTGGAGTACG TGGTGAAAGA
    GGTTTACCTG GCTCTCCTGG GGTAGGCACT GCAGGGTTAC CTGGTGACAA AGGAGAGAGA
    GGTTTTGCTG GAACTCCAGG TTTACCAGGT CTTCCAGGAA CAAAAGGTGA AGCAGGACGA
    ACTGTACCAC TCCCTGGACC TCCTGGGGCA GATGGCCTTC CTGGACCACC TGGTTTCCCT
    GGACCCCAAG GTGACAAAGG GAATCCTGGG CTTCCGGGGA GACCTGGCCT GCCAGGAGAA
    AAAGGTGCTG TTGGGCAGCC AGGTATAGGG TTTCCTGGAC CTCCAGGACC CAAAGGTTTC
    CCAGGTCAGC CTGGCTCACC TGGTCCTCCG GGTACTCCTG GAACCCCTGG CCTGGATGGT
    TTTCCAGGCA CACCAGGTTT ACAAGGTCAA AAAGGTGAAC CTGGAGTGGG TCTCCCTGGT
    CCTAAAGGGT TACCAGGCAT CCCAGGTCCA GCTGGCATCC CTGGAGAAAA AGGAAATCCA
    GGGCTGCCTG GCTTACGAGG TGAACAAGGA TTCCCAGGTG TACCTGGACA ACAAGGATTT
    AGAGGAGATC CAGGCCTCCC TGGACTACAG GGCTTGCCAG GCCCCCAGGG CTCACCAGGA
    GTGGGCCCAC CGGGATTGCC AGGGCCCGCT GGGCAGCCAG GACCTGAGGG GCCACCAGGA
    TTTCCTGGAA TAAAAGGAGA AAGGGGCTTC CCTGGTATCC CAGGTCTAGA TATGCCAGGG
    CCAAAAGGAG ATAAAGGTAG CCAGGGCCAG CCTGGAGTAC CAGGGTCTGT GGGGCTACCT
    GGCCTGCCAG GTCCACAAGG ATCTGTTGGA CTGAAAGGAT CAGCAGGAGC CAAAGGAGAA
    ATGGGAGTTA TGGGAACTCC AGGGTTGCCA GGGACTCCTG GACCAGTTGG AGATCGAGGG
    TTGCCTGGTG AAAAAGGTAG CCAAGGTTTG AAAGGTGTGG TTGGACCACA GGGTGAACCA
    GGTGTCAAAG GGGATAAAGG TGAAGCTGGT CTTCCGGGAA TACCTGGAAG CATGGACACC
    ATGGACATGG TTAGTCTCAA AGGCCAAAAG GGAGATCCTG GAGAAAAAGG GGACCTTGGT
    CCAACAGGAG AAAAAGGGTT ACGTGGGGAG TATGGAGAAC CAGGAATTCC AGGAAGGGAT
    GGTGAACCTG GTACTCCGGG ACAACCAGGA CCAAAAGGAG ATCAAGGCCC CCCAGGGTAC
    CCAGGAGATC CAGGAATTCC AGGGCAGAAA GGATCAGTTG GTGAAATGGG TTTGCCAGGA
    ACACCTGGAG AAAAGGGTCT GCCTGGAATA CCAGGTTCAC CAGGCACACC AGGGTTCCCT
    GGGCAAAAAG GTGAAAAAGG AGACAAAGGA GCAGCAGGTT TTCCTGGAAT TGGCTTTCCA
    GGATCTCCTG GTGAGAAGGG AGAACCAGGA AGAACAGGCA GTCCGGGTTT GTCTGGAGAC
    AAAGGTGAAA AGGGTAATGC AGGAATTCCA GGAATGCCGG GTGCTCCAGG TCCCAAAGGA
    TCCCCTGGCA TTGCAGGATT TCCAGGGAGC CCAGGCCTCC ATGGAGCAAA GGGTGAAAAA
    GGACTTCCTG GCTTAAGTGG CATCCCAGGC TTAAAAGGAG AACCAGGTGA ACCTGGTGTT
    CCTGGTCCTG CTGGTTCCAT TGGTCAGAAG GGTGAACCAG GCTATGATGG GATTCCAGGC
    ACAGCTGGTG CAAAGGGTGA ACAAGGTGTA CCAGGTAGAG GAGTTCCAGG ATATCCAGGT
    GCAAAGGGAG ATAAAGGTGC AAAGGGTGAA GTGGGTTTCC CAGGATCCCC AGGAATTCCA
    GGGATTCCAG GCTTAAAAGG AGAACAAGGG TATGCTGGCC CACCCGGCCC TAAAGGCAAC
    CAAGGTCTTC CTGGACTACC AGGAAGTGCA ATTGAGGGCC CTAAAGGAGA CAGAGGACCA
    CAAGGCCAAC CCGGCCTTCC AGGTTTACCA GGTCCAACGG GGCCACCAGG ACCTCCAGGT
    CTGAAGGGAG CTAAAGGAGA GCAGGGAAGC GATGGCTGGC CTGGAACTCC TGGAGGTCCA
    GGAGATAAAG GCGATCCAGG TTTCCAGGGA CTGCCAGGTA GTCCTGGTTT GCCAGGAGGT
    GCTGGTGAAA AGGGTGATCT AGGGCCTCCA GGTGTTCCAG GTCCAAAAGG ATCTGCAGGC
    TTCCCAGGCC TCAAGGGTGA GCGAGGTGAC CAAGGTCTTC CTGGATCTAA AGGTTTACAA
    GGCCCGCCGG GCCCCCCAGG TGCTTTTCAG CTTATCAAAG GAGATCCTGG CTTACCTGGC
    CCGGTAGGAC CAGTTGGTCT CAAAGGATTC CCAGGACATC CAGGTCCCAA AGGACAGCAA
    GGAGTAACAG GGCCTTCAGG TGTACCTGGA CCACCTGGTA GTCCAGGGTT TGATGGCAGA
    CCAGGGCAAA AAGGAGAAGT TGGTCCTTAT GGTCCTCCAG GGCCCAGAGG AATCCCTGGT
    CCGCCTGGCC CTGAAGGTTT GCCTGGGGCT ATGGGTCCAC CAGGTGCAGC ATCTGTTGCT
    CATGGCTTCC TTGTCACCAG ACACAGTCAA ACCACTGAAG AACCACAATG TCCATTTGGA
    ACAAGGCTGA TTTACCATGG CTACTCCCTT CTTTATGTAC AAGGAAATGA AAGAGCACAC
    GGTCAAGATC TGGGCACTGC AGGGAGCTGT CTTCGTAAAT TTAGTACTAT GCCTTTCTTA
    TTTTGCAATA TCAACAATGT ATGTAACTTT GCATCAAGGA ATGACTATTC CTACTGGCTG
    TCCACTCCTG AACCCATGCC AATGAGTATG GCACCTATCA CTGGTGAAAG TATCAGACCA
    TTCATCAGCA GATGCTCTGT GTGTGAAGCT CCTGCTATGG TAATCGCCGT TCACAGTCAA
    ACAATCCAGA TACCACCATG TCCTGAAGGC TGGAGTTCCC TTTGGATTGG TTACTCCTTT
    GTCATGCACA CCAGTGCTGG TGCTGAGGGC TCTGGCCAGG CCCTGGCGTC TCCTGGTTCC
    TGCCTGGAGG AGTTCCGCAG CGCTCCCTTC ATTGAATGCC ATGGCCGGGG CACCTGCAAT
    TATTATGCAA ATGCTTACAG CTTCTGGCTT GCCACTATAG AGAGGAATGA GATGTTCAAG
    AAACCGACTC CGTCAACTTT GAAAGCAGGG GATCTGCGCT CCAACGTTAG CCGTTGTCAA
    GTCTGTATGA GAAATACATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30658
    Clone ID Related Accession (Same CDS sequence) XM_015277824.2 , XM_015277824.1
    Accession Version XM_015277824.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5001bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product collagen alpha-1(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763585.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTGGCGTT GGCTGGGCTT CGGGCTGCTG CTGAGCGCCG AGCTCAGCGC CGTGTCCGCC 
    GCGGGTTGTT CTGGTTGTGG AAAATGTGAC TGCAGTGGAG TAAAAGGACA AAAGGGAGAA
    AGAGGCCTTC CGGGACTACA GGGTGTGATT GGGTTTCCTG GAATGCAAGG ACCTGAAGGT
    CCCCCTGGGC CACCAGGACT TAAGGGTGAT ACTGGAGAAC CAGGGCTGCC AGGAACAAAA
    GGAACAAGAG GCCCACCAGG ACCATCAGGA TTTCCAGGAA ACCCAGGCCT CCCTGGCATT
    CCTGGACAAG ATGGCCCTCC TGGTCCACCT GGCATTCCAG GATGTAATGG CACAAAGGGT
    GAAAGAGGTC CAGTAGGTCC CCCAGGTTTA CCGGGACTGA CTGGAAGCAT AGGACCCCCA
    GGACCTCCTG GAATTAAGGG AGATCCAGGT GAAGTGATTG GTATTTTACC TCCTAGTGTG
    CTAAAAGGTG ATAAAGGCTT TCCTGGCCAA CCTGGACTAC CTGGTCCACC TGGAACTCCA
    GGGTTTCAGG GACCAATGGG GCCACCAGGG ATTCCAGGAG AACCAGGTCC CCCCGGGCCA
    CCAGGACCCC CAGGCGAGAA GGGCTACATG GGCTTGGGCT TTCAAGGTCC CAAAGGTGAA
    AAGGGAGAAC AAGGGGTCCA GGGTCCCCCG GGGCCACCAG GACCAGCACC ACACATGAAA
    GAAAAAGGAA GCGAAATCAT AAAGGGAGAA AAGGGTGAGC CAGGTGAAAA GGGTGAACAG
    GGAGTCCCAG GATTGCCAGG CTCAGGTTAC AGAGGAGAAA AGGGTGAACC TGGGAAGCCC
    GGTCCACGTG GAAAGCCTGG AAAAGATGGT GAACCAGGAC CAAAAGGAGA ACCCGGCTTG
    CCTGGAGGGT TTGGGATTCC AGGAAGGCCT GGGGAACCTG GTGCTAAGGG TGAAAAAGGT
    GATCAAGGTC CTCAAGGGCC TCCAGGAAGA ATTATAGATG CTGGACCAAT AGATACTTTT
    GGTGAAAAAG GAGACCCTGG AGTTTCAGGT TTGCCTGGAC TAAAAGGTCA AAAAGGTGAA
    AAAGGTTTCC CTGGTGCACA AGGGATCCCA GGAATTCCAG GTCGACCAGT TCCAGACATG
    GTAGGATCAC CTGGTGTCCC TGGCCAGAGA GGTGAAAAAG GTGAAAAGGG TTCTCCAGGA
    TTCCCTATAC CTGGACCTCC TGGAAGAGAT GGTTTCCCAG GCCCACCCGG GTTGCCCGGC
    CCACCAGGTC CTCCAGGTGT AACAGATGGA ATAGATCAAT GTCAGCAAGG GCAACCTGGT
    GCACCAGGTG TACCTGGACC TCCAGGGCAA CATGGATTTC CAGGAGAGAT GGGAGAGAAA
    GGTGAAAAAG GTGAAACCTG TGCCTTGTGT GATAAAGTAG GACCCCCTGG ACTTCCAGGA
    CCACAAGGAC TACCGGGTCC AGCAGGTTTT CCGGGACAGT CAGGTTTCAA GGGTGACAGA
    GGCTTACCTG GACTTGATGG CCTTCCAGGC GTACCCGGGC CCCCAGGCGC TCCAGGACTC
    ATTGGCCTCC CTGGGGCAAA AGGAGAACCA GGAGACTTTA CTTATGATTC CATCCTGAAA
    GGTGAGAAGG GAGATCCTGG TTTCCCAGGA CAACCAGGTA TTCCTGGAAG AGAAGGAACA
    CCAGGAAAAG ACGGATTGCC AGGTCCTCAA GGTCCAAAAG GAGCATCGGG TACAGCTGGC
    TTGAAAGGAG AACGTGGTCC CCCTGGACAA CCTGGATTCC CTGGAGTACG TGGTGAAAGA
    GGTTTACCTG GCTCTCCTGG GGTAGGCACT GCAGGGTTAC CTGGTGACAA AGGAGAGAGA
    GGTTTTGCTG GAACTCCAGG TTTACCAGGT CTTCCAGGAA CAAAAGGTGA AGCAGGACGA
    ACTGTACCAC TCCCTGGACC TCCTGGGGCA GATGGCCTTC CTGGACCACC TGGTTTCCCT
    GGACCCCAAG GTGACAAAGG GAATCCTGGG CTTCCGGGGA GACCTGGCCT GCCAGGAGAA
    AAAGGTGCTG TTGGGCAGCC AGGTATAGGG TTTCCTGGAC CTCCAGGACC CAAAGGTTTC
    CCAGGTCAGC CTGGCTCACC TGGTCCTCCG GGTACTCCTG GAACCCCTGG CCTGGATGGT
    TTTCCAGGCA CACCAGGTTT ACAAGGTCAA AAAGGTGAAC CTGGAGTGGG TCTCCCTGGT
    CCTAAAGGGT TACCAGGCAT CCCAGGTCCA GCTGGCATCC CTGGAGAAAA AGGAAATCCA
    GGGCTGCCTG GCTTACGAGG TGAACAAGGA TTCCCAGGTG TACCTGGACA ACAAGGATTT
    AGAGGAGATC CAGGCCTCCC TGGACTACAG GGCTTGCCAG GCCCCCAGGG CTCACCAGGA
    GTGGGCCCAC CGGGATTGCC AGGGCCCGCT GGGCAGCCAG GACCTGAGGG GCCACCAGGA
    TTTCCTGGAA TAAAAGGAGA AAGGGGCTTC CCTGGTATCC CAGGTCTAGA TATGCCAGGG
    CCAAAAGGAG ATAAAGGTAG CCAGGGCCAG CCTGGAGTAC CAGGGTCTGT GGGGCTACCT
    GGCCTGCCAG GTCCACAAGG ATCTGTTGGA CTGAAAGGAT CAGCAGGAGC CAAAGGAGAA
    ATGGGAGTTA TGGGAACTCC AGGGTTGCCA GGGACTCCTG GACCAGTTGG AGATCGAGGG
    TTGCCTGGTG AAAAAGGTAG CCAAGGTTTG AAAGGTGTGG TTGGACCACA GGGTGAACCA
    GGTGTCAAAG GGGATAAAGG TGAAGCTGGT CTTCCGGGAA TACCTGGAAG CATGGACACC
    ATGGACATGG TTAGTCTCAA AGGCCAAAAG GGAGATCCTG GAGAAAAAGG GGACCTTGGT
    CCAACAGGAG AAAAAGGGTT ACGTGGGGAG TATGGAGAAC CAGGAATTCC AGGAAGGGAT
    GGTGAACCTG GTACTCCGGG ACAACCAGGA CCAAAAGGAG ATCAAGGCCC CCCAGGGTAC
    CCAGGAGATC CAGGAATTCC AGGGCAGAAA GGATCAGTTG GTGAAATGGG TTTGCCAGGA
    ACACCTGGAG AAAAGGGTCT GCCTGGAATA CCAGGTTCAC CAGGCACACC AGGGTTCCCT
    GGGCAAAAAG GTGAAAAAGG AGACAAAGGA GCAGCAGGTT TTCCTGGAAT TGGCTTTCCA
    GGATCTCCTG GTGAGAAGGG AGAACCAGGA AGAACAGGCA GTCCGGGTTT GTCTGGAGAC
    AAAGGTGAAA AGGGTAATGC AGGAATTCCA GGAATGCCGG GTGCTCCAGG TCCCAAAGGA
    TCCCCTGGCA TTGCAGGATT TCCAGGGAGC CCAGGCCTCC ATGGAGCAAA GGGTGAAAAA
    GGACTTCCTG GCTTAAGTGG CATCCCAGGC TTAAAAGGAG AACCAGGTGA ACCTGGTGTT
    CCTGGTCCTG CTGGTTCCAT TGGTCAGAAG GGTGAACCAG GCTATGATGG GATTCCAGGC
    ACAGCTGGTG CAAAGGGTGA ACAAGGTGTA CCAGGTAGAG GAGTTCCAGG ATATCCAGGT
    GCAAAGGGAG ATAAAGGTGC AAAGGGTGAA GTGGGTTTCC CAGGATCCCC AGGAATTCCA
    GGGATTCCAG GCTTAAAAGG AGAACAAGGG TATGCTGGCC CACCCGGCCC TAAAGGCAAC
    CAAGGTCTTC CTGGACTACC AGGAAGTGCA ATTGAGGGCC CTAAAGGAGA CAGAGGACCA
    CAAGGCCAAC CCGGCCTTCC AGGTTTACCA GGTCCAACGG GGCCACCAGG ACCTCCAGGT
    CTGAAGGGAG CTAAAGGAGA GCAGGGAAGC GATGGCTGGC CTGGAACTCC TGGAGGTCCA
    GGAGATAAAG GCGATCCAGG TTTCCAGGGA CTGCCAGGTA GTCCTGGTTT GCCAGGAGGT
    GCTGGTGAAA AGGGTGATCT AGGGCCTCCA GGTGTTCCAG GTCCAAAAGG ATCTGCAGGC
    TTCCCAGGCC TCAAGGGTGA GCGAGGTGAC CAAGGTCTTC CTGGATCTAA AGGTTTACAA
    GGCCCGCCGG GCCCCCCAGG TGCTTTTCAG CTTATCAAAG GAGATCCTGG CTTACCTGGC
    CCGGTAGGAC CAGTTGGTCT CAAAGGATTC CCAGGACATC CAGGTCCCAA AGGACAGCAA
    GGAGTAACAG GGCCTTCAGG TGTACCTGGA CCACCTGGTA GTCCAGGGTT TGATGGCAGA
    CCAGGGCAAA AAGGAGAAGT TGGTCCTTAT GGTCCTCCAG GGCCCAGAGG AATCCCTGGT
    CCGCCTGGCC CTGAAGGTTT GCCTGGGGCT ATGGGTCCAC CAGGTGCAGC ATCTGTTGCT
    CATGGCTTCC TTGTCACCAG ACACAGTCAA ACCACTGAAG AACCACAATG TCCATTTGGA
    ACAAGGCTGA TTTACCATGG CTACTCCCTT CTTTATGTAC AAGGAAATGA AAGAGCACAC
    GGTCAAGATC TGGGCACTGC AGGGAGCTGT CTTCGTAAAT TTAGTACTAT GCCTTTCTTA
    TTTTGCAATA TCAACAATGT ATGTAACTTT GCATCAAGGA ATGACTATTC CTACTGGCTG
    TCCACTCCTG AACCCATGCC AATGAGTATG GCACCTATCA CTGGTGAAAG TATCAGACCA
    TTCATCAGCA GATGCTCTGT GTGTGAAGCT CCTGCTATGG TAATCGCCGT TCACAGTCAA
    ACAATCCAGA TACCACCATG TCCTGAAGGC TGGAGTTCCC TTTGGATTGG TTACTCCTTT
    GTCATGCACA CCAGTGCTGG TGCTGAGGGC TCTGGCCAGG CCCTGGCGTC TCCTGGTTCC
    TGCCTGGAGG AGTTCCGCAG CGCTCCCTTC ATTGAATGCC ATGGCCGGGG CACCTGCAAT
    TATTATGCAA ATGCTTACAG CTTCTGGCTT GCCACTATAG AGAGGAATGA GATGTTCAAG
    AAACCGACTC CGTCAACTTT GAAAGCAGGG GATCTGCGCT CCAACGTTAG CCGTTGTCAA
    GTCTGTATGA GAAATACATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133310.1
    CDS120..5120
    Translation

    Target ORF information:

    RefSeq Version XM_015277824.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen, type IV, alpha 1 (COL4A1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277824.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTGGCGTT GGCTGGGCTT CGGGCTGCTG CTGAGCGCCG AGCTCAGCGC CGTGTCCGCC 
    GCGGGTTGTT CTGGTTGTGG AAAATGTGAC TGCAGTGGAG TAAAAGGACA AAAGGGAGAA
    AGAGGCCTTC CGGGACTACA GGGTGTGATT GGGTTTCCTG GAATGCAAGG ACCTGAAGGT
    CCCCCTGGGC CACCAGGACT TAAGGGTGAT ACTGGAGAAC CAGGGCTGCC AGGAACAAAA
    GGAACAAGAG GCCCACCAGG ACCATCAGGA TTTCCAGGAA ACCCAGGCCT CCCTGGCATT
    CCTGGACAAG ATGGCCCTCC TGGTCCACCT GGCATTCCAG GATGTAATGG CACAAAGGGT
    GAAAGAGGTC CAGTAGGTCC CCCAGGTTTA CCGGGACTGA CTGGAAGCAT AGGACCCCCA
    GGACCTCCTG GAATTAAGGG AGATCCAGGT GAAGTGATTG GTATTTTACC TCCTAGTGTG
    CTAAAAGGTG ATAAAGGCTT TCCTGGCCAA CCTGGACTAC CTGGTCCACC TGGAACTCCA
    GGGTTTCAGG GACCAATGGG GCCACCAGGG ATTCCAGGAG AACCAGGTCC CCCCGGGCCA
    CCAGGACCCC CAGGCGAGAA GGGCTACATG GGCTTGGGCT TTCAAGGTCC CAAAGGTGAA
    AAGGGAGAAC AAGGGGTCCA GGGTCCCCCG GGGCCACCAG GACCAGCACC ACACATGAAA
    GAAAAAGGAA GCGAAATCAT AAAGGGAGAA AAGGGTGAGC CAGGTGAAAA GGGTGAACAG
    GGAGTCCCAG GATTGCCAGG CTCAGGTTAC AGAGGAGAAA AGGGTGAACC TGGGAAGCCC
    GGTCCACGTG GAAAGCCTGG AAAAGATGGT GAACCAGGAC CAAAAGGAGA ACCCGGCTTG
    CCTGGAGGGT TTGGGATTCC AGGAAGGCCT GGGGAACCTG GTGCTAAGGG TGAAAAAGGT
    GATCAAGGTC CTCAAGGGCC TCCAGGAAGA ATTATAGATG CTGGACCAAT AGATACTTTT
    GGTGAAAAAG GAGACCCTGG AGTTTCAGGT TTGCCTGGAC TAAAAGGTCA AAAAGGTGAA
    AAAGGTTTCC CTGGTGCACA AGGGATCCCA GGAATTCCAG GTCGACCAGT TCCAGACATG
    GTAGGATCAC CTGGTGTCCC TGGCCAGAGA GGTGAAAAAG GTGAAAAGGG TTCTCCAGGA
    TTCCCTATAC CTGGACCTCC TGGAAGAGAT GGTTTCCCAG GCCCACCCGG GTTGCCCGGC
    CCACCAGGTC CTCCAGGTGT AACAGATGGA ATAGATCAAT GTCAGCAAGG GCAACCTGGT
    GCACCAGGTG TACCTGGACC TCCAGGGCAA CATGGATTTC CAGGAGAGAT GGGAGAGAAA
    GGTGAAAAAG GTGAAACCTG TGCCTTGTGT GATAAAGTAG GACCCCCTGG ACTTCCAGGA
    CCACAAGGAC TACCGGGTCC AGCAGGTTTT CCGGGACAGT CAGGTTTCAA GGGTGACAGA
    GGCTTACCTG GACTTGATGG CCTTCCAGGC GTACCCGGGC CCCCAGGCGC TCCAGGACTC
    ATTGGCCTCC CTGGGGCAAA AGGAGAACCA GGAGACTTTA CTTATGATTC CATCCTGAAA
    GGTGAGAAGG GAGATCCTGG TTTCCCAGGA CAACCAGGTA TTCCTGGAAG AGAAGGAACA
    CCAGGAAAAG ACGGATTGCC AGGTCCTCAA GGTCCAAAAG GAGCATCGGG TACAGCTGGC
    TTGAAAGGAG AACGTGGTCC CCCTGGACAA CCTGGATTCC CTGGAGTACG TGGTGAAAGA
    GGTTTACCTG GCTCTCCTGG GGTAGGCACT GCAGGGTTAC CTGGTGACAA AGGAGAGAGA
    GGTTTTGCTG GAACTCCAGG TTTACCAGGT CTTCCAGGAA CAAAAGGTGA AGCAGGACGA
    ACTGTACCAC TCCCTGGACC TCCTGGGGCA GATGGCCTTC CTGGACCACC TGGTTTCCCT
    GGACCCCAAG GTGACAAAGG GAATCCTGGG CTTCCGGGGA GACCTGGCCT GCCAGGAGAA
    AAAGGTGCTG TTGGGCAGCC AGGTATAGGG TTTCCTGGAC CTCCAGGACC CAAAGGTTTC
    CCAGGTCAGC CTGGCTCACC TGGTCCTCCG GGTACTCCTG GAACCCCTGG CCTGGATGGT
    TTTCCAGGCA CACCAGGTTT ACAAGGTCAA AAAGGTGAAC CTGGAGTGGG TCTCCCTGGT
    CCTAAAGGGT TACCAGGCAT CCCAGGTCCA GCTGGCATCC CTGGAGAAAA AGGAAATCCA
    GGGCTGCCTG GCTTACGAGG TGAACAAGGA TTCCCAGGTG TACCTGGACA ACAAGGATTT
    AGAGGAGATC CAGGCCTCCC TGGACTACAG GGCTTGCCAG GCCCCCAGGG CTCACCAGGA
    GTGGGCCCAC CGGGATTGCC AGGGCCCGCT GGGCAGCCAG GACCTGAGGG GCCACCAGGA
    TTTCCTGGAA TAAAAGGAGA AAGGGGCTTC CCTGGTATCC CAGGTCTAGA TATGCCAGGG
    CCAAAAGGAG ATAAAGGTAG CCAGGGCCAG CCTGGAGTAC CAGGGTCTGT GGGGCTACCT
    GGCCTGCCAG GTCCACAAGG ATCTGTTGGA CTGAAAGGAT CAGCAGGAGC CAAAGGAGAA
    ATGGGAGTTA TGGGAACTCC AGGGTTGCCA GGGACTCCTG GACCAGTTGG AGATCGAGGG
    TTGCCTGGTG AAAAAGGTAG CCAAGGTTTG AAAGGTGTGG TTGGACCACA GGGTGAACCA
    GGTGTCAAAG GGGATAAAGG TGAAGCTGGT CTTCCGGGAA TACCTGGAAG CATGGACACC
    ATGGACATGG TTAGTCTCAA AGGCCAAAAG GGAGATCCTG GAGAAAAAGG GGACCTTGGT
    CCAACAGGAG AAAAAGGGTT ACGTGGGGAG TATGGAGAAC CAGGAATTCC AGGAAGGGAT
    GGTGAACCTG GTACTCCGGG ACAACCAGGA CCAAAAGGAG ATCAAGGCCC CCCAGGGTAC
    CCAGGAGATC CAGGAATTCC AGGGCAGAAA GGATCAGTTG GTGAAATGGG TTTGCCAGGA
    ACACCTGGAG AAAAGGGTCT GCCTGGAATA CCAGGTTCAC CAGGCACACC AGGGTTCCCT
    GGGCAAAAAG GTGAAAAAGG AGACAAAGGA GCAGCAGGTT TTCCTGGAAT TGGCTTTCCA
    GGATCTCCTG GTGAGAAGGG AGAACCAGGA AGAACAGGCA GTCCGGGTTT GTCTGGAGAC
    AAAGGTGAAA AGGGTAATGC AGGAATTCCA GGAATGCCGG GTGCTCCAGG TCCCAAAGGA
    TCCCCTGGCA TTGCAGGATT TCCAGGGAGC CCAGGCCTCC ATGGAGCAAA GGGTGAAAAA
    GGACTTCCTG GCTTAAGTGG CATCCCAGGC TTAAAAGGAG AACCAGGTGA ACCTGGTGTT
    CCTGGTCCTG CTGGTTCCAT TGGTCAGAAG GGTGAACCAG GCTATGATGG GATTCCAGGC
    ACAGCTGGTG CAAAGGGTGA ACAAGGTGTA CCAGGTAGAG GAGTTCCAGG ATATCCAGGT
    GCAAAGGGAG ATAAAGGTGC AAAGGGTGAA GTGGGTTTCC CAGGATCCCC AGGAATTCCA
    GGGATTCCAG GCTTAAAAGG AGAACAAGGG TATGCTGGCC CACCCGGCCC TAAAGGCAAC
    CAAGGTCTTC CTGGACTACC AGGAAGTGCA ATTGAGGGCC CTAAAGGAGA CAGAGGACCA
    CAAGGCCAAC CCGGCCTTCC AGGTTTACCA GGTCCAACGG GGCCACCAGG ACCTCCAGGT
    CTGAAGGGAG CTAAAGGAGA GCAGGGAAGC GATGGCTGGC CTGGAACTCC TGGAGGTCCA
    GGAGATAAAG GCGATCCAGG TTTCCAGGGA CTGCCAGGTA GTCCTGGTTT GCCAGGAGGT
    GCTGGTGAAA AGGGTGATCT AGGGCCTCCA GGTGTTCCAG GTCCAAAAGG ATCTGCAGGC
    TTCCCAGGCC TCAAGGGTGA GCGAGGTGAC CAAGGTCTTC CTGGATCTAA AGGTTTACAA
    GGCCCGCCGG GCCCCCCAGG TGCTTTTCAG CTTATCAAAG GAGATCCTGG CTTACCTGGC
    CCGGTAGGAC CAGTTGGTCT CAAAGGATTC CCAGGACATC CAGGTCCCAA AGGACAGCAA
    GGAGTAACAG GGCCTTCAGG TGTACCTGGA CCACCTGGTA GTCCAGGGTT TGATGGCAGA
    CCAGGGCAAA AAGGAGAAGT TGGTCCTTAT GGTCCTCCAG GGCCCAGAGG AATCCCTGGT
    CCGCCTGGCC CTGAAGGTTT GCCTGGGGCT ATGGGTCCAC CAGGTGCAGC ATCTGTTGCT
    CATGGCTTCC TTGTCACCAG ACACAGTCAA ACCACTGAAG AACCACAATG TCCATTTGGA
    ACAAGGCTGA TTTACCATGG CTACTCCCTT CTTTATGTAC AAGGAAATGA AAGAGCACAC
    GGTCAAGATC TGGGCACTGC AGGGAGCTGT CTTCGTAAAT TTAGTACTAT GCCTTTCTTA
    TTTTGCAATA TCAACAATGT ATGTAACTTT GCATCAAGGA ATGACTATTC CTACTGGCTG
    TCCACTCCTG AACCCATGCC AATGAGTATG GCACCTATCA CTGGTGAAAG TATCAGACCA
    TTCATCAGCA GATGCTCTGT GTGTGAAGCT CCTGCTATGG TAATCGCCGT TCACAGTCAA
    ACAATCCAGA TACCACCATG TCCTGAAGGC TGGAGTTCCC TTTGGATTGG TTACTCCTTT
    GTCATGCACA CCAGTGCTGG TGCTGAGGGC TCTGGCCAGG CCCTGGCGTC TCCTGGTTCC
    TGCCTGGAGG AGTTCCGCAG CGCTCCCTTC ATTGAATGCC ATGGCCGGGG CACCTGCAAT
    TATTATGCAA ATGCTTACAG CTTCTGGCTT GCCACTATAG AGAGGAATGA GATGTTCAAG
    AAACCGACTC CGTCAACTTT GAAAGCAGGG GATCTGCGCT CCAACGTTAG CCGTTGTCAA
    GTCTGTATGA GAAATACATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.