RHOA cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	RHOA
Entrez Gene ID	395442
Full Name	ras homolog family member A
General protein information	Preferred Names ras homolog family member A Names transforming protein RhoA RhoA GTPase ras homolog gene family, member A
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025154420.1	XP_025010188.1	transforming protein RhoA isoform X1
NM_204704.1	NP_990035.1	transforming protein RhoA
XM_025154419.1	XP_025010187.1	transforming protein RhoA isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	RHOA	NP_001655.1
Canis lupus familiaris (dog)	RHOA	NP_001003273.2
Mus musculus (house mouse)	Rhoa	NP_058082.2
Gallus gallus (chicken)	RHOA	NP_990035.1
Eremothecium gossypii	AGOS_ABR183W	NP_983131.1
Schizosaccharomyces pombe (fission yeast)	rho1	NP_594490.1
Neurospora crassa	NCU01484	XP_956761.2
Pan troglodytes (chimpanzee)	RHOA	XP_003950161.1
Bos taurus (cattle)	RHOA	NP_788818.1
Rattus norvegicus (Norway rat)	Rhoa	NP_476473.1
Danio rerio (zebrafish)	rhoab	NP_997914.2
Kluyveromyces lactis	KLLA0B10626g	XP_452002.1
Magnaporthe oryzae (rice blast fungus)	MGG_07176	XP_003715379.1
Schizosaccharomyces pombe (fission yeast)	rho5	NP_593631.1
Saccharomyces cerevisiae (baker's yeast)	RHO1	NP_015491.1
Xenopus tropicalis (tropical clawed frog)	rhoa.2	NP_001016648.1

Gene Ontology
Bibliography

Related articles in PubMed

Asymmetric localization of DLC1 defines avian trunk neural crest polarity for directional delamination and migration.
Liu JA, Rao Y, Cheung MPL, Hui MN, Wu MH, Chan LK, Ng IO, Niu B, Cheah KSE, Sharma R, Hodgson L, Cheung M
Nature communications8(1)1185(2017 Oct)

RHOA-ROCK signalling is necessary for lateralization and differentiation of the developing sinoatrial node.
Vicente-Steijn R, Kelder TP, Tertoolen LG, Wisse LJ, Pijnappels DA, Poelmann RE, Schalij MJ, deRuiter MC, Gittenberger-de Groot AC, Jongbloed MRM
Cardiovascular research113(10)1186-1197(2017 Aug)

Melanosome transfer to keratinocyte in the chicken embryonic skin is mediated by vesicle release associated with Rho-regulated membrane blebbing.
Tadokoro R, Murai H, Sakai KI, Okui T, Yokota Y, Takahashi Y
Scientific reports638277(2016 Dec)

Effect of HMG-CoA Reductase Inhibition on Vascular Smooth Muscle Cells Extracellular Matrix Production: Role of RhoA.
Palomino-Morales R, Perales S, Torres C, Linares A, Alejandre MJ
Current vascular pharmacology14(4)345-52(2016)

Fluid flow forces and rhoA regulate fibrous development of the atrioventricular valves.
Tan H, Biechler S, Junor L, Yost MJ, Dean D, Li J, Potts JD, Goodwin RL
Developmental biology374(2)345-56(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Statins regulate the activation of RhoA through its isoprenylation, which is crucial for the regulation of extracellular matrix synthesis in smooth muscle cells.
Title: Effect of HMG-CoA Reductase Inhibition on Vascular Smooth Muscle Cells Extracellular Matrix Production: Role of RhoA.

Data indicate that RhoA is upregulated and activated in 7 day flow cultures.
Title: Fluid flow forces and rhoA regulate fibrous development of the atrioventricular valves.

miR-34a regulates RhoA/Rac1 cross-talk and negatively modulates reorganization of the actin cytoskeleton.
Title: MicroRNA-34a modulates cytoskeletal dynamics through regulating RhoA/Rac1 cross-talk in chondroblasts.

RhoA uses other pathway than ROCK/MLC in the modulation of actin stress fibers and chondrogenesis.
Title: Inhibition of RhoA but not ROCK induces chondrogenesis of chick limb mesenchymal cells.

Data show that Rho GTPases expression at the apical portion of the lens placode occurs with the same dynamics as that of the cytoskeleton.
Title: Rho signaling pathway and apical constriction in the early lens placode.

The following RHOA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RHOA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa24857	NM_204704.1 Latest version!	Gallus gallus ras homolog family member A (RHOA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OGa143879	XM_025154419.1 Latest version!	Gallus gallus ras homolog family member A (RHOA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OGa143879	XM_025154420.1 Latest version!	Gallus gallus ras homolog family member A (RHOA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RHOA ( NM_204704.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990035.1 Gallus gallus ras homolog family member A (RHOA), mRNA.

Price & Availability↑

CloneID	OGa24857
Clone ID Related Accession (Same CDS sequence)	NM_204704.1
Accession Version	NM_204704.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Gallus gallus(chicken)
Product	transforming protein RhoA
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ294705.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AJ294705.1, CV861004.1 [ECO:0000331] ##Evidence-Data-END##

ATGGCAGCCA TTCGAAAAAA GCTGGTCATA GTGGGCGACG GTGCCTGCGG GAAGACCTGT 
CTGCTGATTG TGTTTAGCAA AGACCAGTTC CCTGAAGTCT ACGTTCCCAC CGTCTTTGAA 
AATTATGTAG CAGATATTGA AGTGGATGGA AAGCAGGTGG AGTTGGCTTT GTGGGATACA 
GCAGGACAGG AAGACTACGA TCGACTTAGA CCGCTTTCTT ATCCAGATAC TGATGTTATA 
CTTATGTGTT TTTCAATCGA TAGTCCTGAT AGTTTAGAAA ACATCCCGGA GAAGTGGACC 
CCGGAAGTGA AGCATTTCTG TCCCAACGTG CCTATCATCT TGGTAGGAAA CAAGAAGGAC 
CTGAGGAATG ACGAGCACAC AAGACGAGAG CTGGCCAAAA TGAAGCAGGA GCCTGTCAAA 
CCTGAGGAAG GAAGAGATAT GGCAAACCGC ATCGGTGCAT TTGGATATAT GGAGTGTTCG 
GCAAAGACCA AAGACGGTGT GAGGGAGGTT TTTGAAATGG CCACTAGAGC TGCTTTGCAA 
GCCCGGCGTG GCAAGAAAAA GTCCGGGTGC CTTCTCTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990035.1
CDS	49..630
Translation	MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLLL

Target ORF information:

RefSeq Version	NM_204704.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ras homolog family member A (RHOA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204704.1

ORF Insert Sequence:

ATGGCAGCCA TTCGAAAAAA GCTGGTCATA GTGGGCGACG GTGCCTGCGG GAAGACCTGT 
CTGCTGATTG TGTTTAGCAA AGACCAGTTC CCTGAAGTCT ACGTTCCCAC CGTCTTTGAA 
AATTATGTAG CAGATATTGA AGTGGATGGA AAGCAGGTGG AGTTGGCTTT GTGGGATACA 
GCAGGACAGG AAGACTACGA TCGACTTAGA CCGCTTTCTT ATCCAGATAC TGATGTTATA 
CTTATGTGTT TTTCAATCGA TAGTCCTGAT AGTTTAGAAA ACATCCCGGA GAAGTGGACC 
CCGGAAGTGA AGCATTTCTG TCCCAACGTG CCTATCATCT TGGTAGGAAA CAAGAAGGAC 
CTGAGGAATG ACGAGCACAC AAGACGAGAG CTGGCCAAAA TGAAGCAGGA GCCTGTCAAA 
CCTGAGGAAG GAAGAGATAT GGCAAACCGC ATCGGTGCAT TTGGATATAT GGAGTGTTCG 
GCAAAGACCA AAGACGGTGT GAGGGAGGTT TTTGAAATGG CCACTAGAGC TGCTTTGCAA 
GCCCGGCGTG GCAAGAAAAA GTCCGGGTGC CTTCTCTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHOA ( XM_025154420.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025010188.1 Gallus gallus ras homolog family member A (RHOA), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa143879
Clone ID Related Accession (Same CDS sequence)	XM_025154420.1 , XM_025154419.1
Accession Version	XM_025154420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	transforming protein RhoA isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCCA TTCGAAAAAA GCTGGTCATA GTGGGCGACG GTGCCTGCGG GAAGACCTGT 
CTGCTGATTG TGTTTAGCAA AGACCAGTTC CCTGAAGTCT ACGTTCCCAC CGTCTTTGAA 
AATTATGTAG CAGATATTGA AGTGGATGGA AAGCAGGTGG AGTTGGCTTT GTGGGATACA 
GCAGGACAGG AAGACTACGA TCGACTTAGA CCGCTTTCTT ATCCAGATAC TGATGTTATA 
CTTATGTGTT TTTCAATTGA TAGTCCTGAT AGTTTAGAAA ACATCCCAGA GAAGTGGACC 
CCGGAAGTGA AGCATTTCTG TCCCAACGTG CCTATCATCT TGGTAGGAAA CAAGAAGGAC 
CTGAGGAATG ACGAGCACAC AAGACGAGAG CTGGCCAAAA TGAAGCAGGA GCCTGTCAAA 
CCTGAGGAAG GAAGAGATAT GGCAAACCGC ATCGGTGCAT TTGGATATAT GGAGTGTTCG 
GCAAAGACCA AAGACGGTGT GAGGGAGGTT TTTGAAATGG CCACTAGAGC TGCTTTGCAA 
GCCCGGCGTG GCAAGAAAAA GTCCGGGTGC CTTCTCTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025010188.1
CDS	29..610
Translation	MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLLL

Target ORF information:

RefSeq Version	XM_025154420.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ras homolog family member A (RHOA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025154420.1

ORF Insert Sequence:

ATGGCAGCCA TTCGAAAAAA GCTGGTCATA GTGGGCGACG GTGCCTGCGG GAAGACCTGT 
CTGCTGATTG TGTTTAGCAA AGACCAGTTC CCTGAAGTCT ACGTTCCCAC CGTCTTTGAA 
AATTATGTAG CAGATATTGA AGTGGATGGA AAGCAGGTGG AGTTGGCTTT GTGGGATACA 
GCAGGACAGG AAGACTACGA TCGACTTAGA CCGCTTTCTT ATCCAGATAC TGATGTTATA 
CTTATGTGTT TTTCAATTGA TAGTCCTGAT AGTTTAGAAA ACATCCCAGA GAAGTGGACC 
CCGGAAGTGA AGCATTTCTG TCCCAACGTG CCTATCATCT TGGTAGGAAA CAAGAAGGAC 
CTGAGGAATG ACGAGCACAC AAGACGAGAG CTGGCCAAAA TGAAGCAGGA GCCTGTCAAA 
CCTGAGGAAG GAAGAGATAT GGCAAACCGC ATCGGTGCAT TTGGATATAT GGAGTGTTCG 
GCAAAGACCA AAGACGGTGT GAGGGAGGTT TTTGAAATGG CCACTAGAGC TGCTTTGCAA 
GCCCGGCGTG GCAAGAAAAA GTCCGGGTGC CTTCTCTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHOA ( XM_025154419.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025010187.1 Gallus gallus ras homolog family member A (RHOA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa143879
Clone ID Related Accession (Same CDS sequence)	XM_025154420.1 , XM_025154419.1
Accession Version	XM_025154419.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	transforming protein RhoA isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCCA TTCGAAAAAA GCTGGTCATA GTGGGCGACG GTGCCTGCGG GAAGACCTGT 
CTGCTGATTG TGTTTAGCAA AGACCAGTTC CCTGAAGTCT ACGTTCCCAC CGTCTTTGAA 
AATTATGTAG CAGATATTGA AGTGGATGGA AAGCAGGTGG AGTTGGCTTT GTGGGATACA 
GCAGGACAGG AAGACTACGA TCGACTTAGA CCGCTTTCTT ATCCAGATAC TGATGTTATA 
CTTATGTGTT TTTCAATTGA TAGTCCTGAT AGTTTAGAAA ACATCCCAGA GAAGTGGACC 
CCGGAAGTGA AGCATTTCTG TCCCAACGTG CCTATCATCT TGGTAGGAAA CAAGAAGGAC 
CTGAGGAATG ACGAGCACAC AAGACGAGAG CTGGCCAAAA TGAAGCAGGA GCCTGTCAAA 
CCTGAGGAAG GAAGAGATAT GGCAAACCGC ATCGGTGCAT TTGGATATAT GGAGTGTTCG 
GCAAAGACCA AAGACGGTGT GAGGGAGGTT TTTGAAATGG CCACTAGAGC TGCTTTGCAA 
GCCCGGCGTG GCAAGAAAAA GTCCGGGTGC CTTCTCTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025010187.1
CDS	272..853
Translation	MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLLL

Target ORF information:

RefSeq Version	XM_025154419.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ras homolog family member A (RHOA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025154419.1

ORF Insert Sequence:

ATGGCAGCCA TTCGAAAAAA GCTGGTCATA GTGGGCGACG GTGCCTGCGG GAAGACCTGT 
CTGCTGATTG TGTTTAGCAA AGACCAGTTC CCTGAAGTCT ACGTTCCCAC CGTCTTTGAA 
AATTATGTAG CAGATATTGA AGTGGATGGA AAGCAGGTGG AGTTGGCTTT GTGGGATACA 
GCAGGACAGG AAGACTACGA TCGACTTAGA CCGCTTTCTT ATCCAGATAC TGATGTTATA 
CTTATGTGTT TTTCAATTGA TAGTCCTGAT AGTTTAGAAA ACATCCCAGA GAAGTGGACC 
CCGGAAGTGA AGCATTTCTG TCCCAACGTG CCTATCATCT TGGTAGGAAA CAAGAAGGAC 
CTGAGGAATG ACGAGCACAC AAGACGAGAG CTGGCCAAAA TGAAGCAGGA GCCTGTCAAA 
CCTGAGGAAG GAAGAGATAT GGCAAACCGC ATCGGTGCAT TTGGATATAT GGAGTGTTCG 
GCAAAGACCA AAGACGGTGT GAGGGAGGTT TTTGAAATGG CCACTAGAGC TGCTTTGCAA 
GCCCGGCGTG GCAAGAAAAA GTCCGGGTGC CTTCTCTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Asymmetric localization of DLC1 defines avian trunk neural crest polarity for directional delamination and migration.
Nature communications8(1)1185(2017 Oct)
Liu JA,Rao Y,Cheung MPL,Hui MN,Wu MH,Chan LK,Ng IO,Niu B,Cheah KSE,Sharma R,Hodgson L,Cheung M

RHOA-ROCK signalling is necessary for lateralization and differentiation of the developing sinoatrial node.
Cardiovascular research113(10)1186-1197(2017 Aug)
Vicente-Steijn R,Kelder TP,Tertoolen LG,Wisse LJ,Pijnappels DA,Poelmann RE,Schalij MJ,deRuiter MC,Gittenberger-de Groot AC,Jongbloed MRM

Melanosome transfer to keratinocyte in the chicken embryonic skin is mediated by vesicle release associated with Rho-regulated membrane blebbing.
Scientific reports638277(2016 Dec)
Tadokoro R,Murai H,Sakai KI,Okui T,Yokota Y,Takahashi Y

Effect of HMG-CoA Reductase Inhibition on Vascular Smooth Muscle Cells Extracellular Matrix Production: Role of RhoA.
Current vascular pharmacology14(4)345-52(2016)
Palomino-Morales R,Perales S,Torres C,Linares A,Alejandre MJ

Fluid flow forces and rhoA regulate fibrous development of the atrioventricular valves.
Developmental biology374(2)345-56(2013 Feb)
Tan H,Biechler S,Junor L,Yost MJ,Dean D,Li J,Potts JD,Goodwin RL

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RHOA cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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