gRNAs
The following ZP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OGa28351 | |
| Clone ID Related Accession (Same CDS sequence) | NM_204683.2 | |
| Accession Version | NM_204683.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2805bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2015-12-26 | |
| Organism | Gallus gallus(chicken) | |
| Product | zona pellucida sperm-binding protein 1 precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN03004551.1. On Jul 10, 2015 this sequence version replaced NM_204683.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AJ289697.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SRS213969 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGGCCGCA GCCGCTCCCT GCTGCTGCCA TTGCTGCTGC TGCTGCCTGC AGGGCTGCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_990014.2 |
| CDS | 9..2813 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_204683.2 |
1 | ATGGGCCGCA GCCGCTCCCT GCTGCTGCCA TTGCTGCTGC TGCTGCCTGC AGGGCTGCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa49025 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015301442.1 | |
| Accession Version | XM_015301442.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-01-03 | |
| Organism | Gallus gallus(chicken) | |
| Product | zona pellucida sperm-binding protein 1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_460419.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCCGTGCA CCGTGGAGCT GTGGGGTCCT ACGCCGGCAC GGTGCTGCTT CAGCCCCATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015156928.1 |
| CDS | 427..3432 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015301442.1 |
1 | ATGCCGTGCA CCGTGGAGCT GTGGGGTCCT ACGCCGGCAC GGTGCTGCTT CAGCCCCATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa49026 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015301443.1 | |
| Accession Version | XM_015301443.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2877bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-01-03 | |
| Organism | Gallus gallus(chicken) | |
| Product | zona pellucida sperm-binding protein 1 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_460419.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGCCGCA GCTGCTCCCT GCTGCTGCCA TTGCTGCTGC TGCTGCCTGC AGGGCTGCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015156929.1 |
| CDS | 26..2902 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015301443.1 |
1 | ATGGGCCGCA GCTGCTCCCT GCTGCTGCCA TTGCTGCTGC TGCTGCCTGC AGGGCTGCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Heterocomplex formation and cell-surface accumulation of hen's serum zona pellucida B1 (ZPB1)with ZPC expressed by a mammalian cell line (COS-7): a possible initiating step of egg-envelope matrix construction. |
Involvement of interaction of ZP1 and ZPC in the formation of quail perivitelline membrane. |
Interaction of sperm with purified native chicken ZP1 and ZPC proteins. |
The major chicken egg envelope protein ZP1 is different from ZPB and is synthesized in the liver. |
