GJA1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	GJA1
Entrez Gene ID	395278
Full Name	gap junction protein alpha 1
Synonyms	CX43,connexin-43
General protein information	Preferred Names gap junction protein alpha 1 Names gap junction alpha-1 protein connexin 43
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_204586.2	NP_989917.1	gap junction alpha-1 protein

Pathways from BioSystems

Homologies

	GJA1	XP_001109617.1
Mus musculus (house mouse)	Gja1	NP_034418.1
Xenopus tropicalis (tropical clawed frog)	gja1	NP_988856.1
Pan troglodytes (chimpanzee)	GJA1	XP_001164338.1
Canis lupus familiaris (dog)	GJA1	NP_001002951.2
Bos taurus (cattle)	GJA1	NP_776493.1
Rattus norvegicus (Norway rat)	Gja1	NP_036699.1
Homo sapiens (human)	GJA1	NP_000156.1
Gallus gallus (chicken)	GJA1	NP_989917.1
Danio rerio (zebrafish)	cx43	NP_571113.1

Gene Ontology
Bibliography

Related articles in PubMed

Polymorphisms in GJA1 and their association with growth traits in chicken.
Shahjahan M, Liu RR, Zhao GP, Zhang JJ, Zheng MQ, Li QH, Wen J
Genetics and molecular research : GMR14(4)18839-50(2015 Dec)

Adaptation of connexin 43-hemichannel prostaglandin release to mechanical loading.
Siller-Jackson AJ, Burra S, Gu S, Xia X, Bonewald LF, Sprague E, Jiang JX
The Journal of biological chemistry283(39)26374-82(2008 Sep)

Hormonal, pH, and calcium regulation of connexin 43-mediated dye transfer in osteocytes in chick calvaria.
Ishihara Y, Kamioka H, Honjo T, Ueda H, Takano-Yamamoto T, Yamashiro T
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research23(3)350-60(2008 Mar)

Connexin 43 is localized with actin in tenocytes.
Wall ME, Otey C, Qi J, Banes AJ
Cell motility and the cytoskeleton64(2)121-30(2007 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The present study describes four previously unknown SNPs, four haplotypes from two variants having high linkage disequilibrium, and seven diplotypes of the chicken GJA1 gene, and reports their significant association with growth traits.
Title: Polymorphisms in GJA1 and their association with growth traits in chicken.

that Cx43-hemichannel activity associated with PGE(2) release is adaptively regulated by mechanical loading to provide an effective means of regulating levels of extracellular signaling molecules responsible for initiation of bone (re)modeling.
Title: Adaptation of connexin 43-hemichannel prostaglandin release to mechanical loading.

Cell-cell communication among osteocytes in chick calvaria was mediated by Cx43 and was regulated by extracellular pH, extracellular calcium ion concentration, and PTH.
Title: Hormonal, pH, and calcium regulation of connexin 43-mediated dye transfer in osteocytes in chick calvaria.

Four percent of tenocytes had colocalization of actin filaments and Cx43, which was significantly increased with 5% cyclic strain
Title: Connexin 43 is localized with actin in tenocytes.

mechanotransduction occurs in individual cells but integration of signals markedly enhances it and (ii) communication via connexin 43 is inhibitory.
Title: Connexin 32 and 43 gap junctions differentially modulate tenocyte response to cyclic mechanical load.

The following GJA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GJA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa28288	NM_204586.2 Latest version!	Gallus gallus gap junction protein alpha 1 (GJA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GJA1 ( NM_204586.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_989917.1 Gallus gallus gap junction protein alpha 1 (GJA1), mRNA.

Price & Availability↑

CloneID	OGa28288
Clone ID Related Accession (Same CDS sequence)	NM_204586.2
Accession Version	NM_204586.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Gallus gallus(chicken)
Product	gap junction alpha-1 protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M29003.2. On Aug 30, 2012 this sequence version replaced NM_204586.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M29003.2, BU412829.1 [ECO:0000332] ##Evidence-Data-END##

ATGGGTGATT GGAGTGCCCT GGGAAAACTT CTTGACAAGG TTCAAGCTTA TTCTACTGCA 
GGAGGAAAAG TGTGGCTGTC TGTCCTCTTT ATTTTCCGAA TCCTGCTATT GGGGACAGCA 
GTCGAGTCTG CTTGGGGAGA TGAACAGTCT GCTTTTCGGT GCAATACTCA ACAGCCTGGT 
TGTGAGAACG TCTGCTATGA CAAGTCCTTC CCCATCTCCC ACGTGCGTTT CTGGGTTCTG 
CAGATCATAT TTGTGTCTGT ACCAACCCTC TTGTACCTGG CGCACGTGTT CTACGTGATG 
AGGAAAGAAG AGAAGCTGAA CAAAAGAGAG GAAGAGCTCA AGGTGGTCCA GAATGATGGT 
GTGAATGTGG ATATGCACCT CAAACAAATA GAAAGTAAGA AATTCAAGTA TGGAATTGAA 
GAGCACGGCA AGGTAAAGAT GCGTGGAGGA CTGCTTCGTA CTTACATCAT CAGCATCCTG 
TTTAAGTCTG TCTTCGAGGT GGCTTTCCTG CTGATACAGT GGTACATTTA TGGGTTTAGC 
CTGAGTGCCA TCTACACCTG TGAGCGAGAC CCATGCCCTC ACAGGGTGGA CTGTTTCCTG 
TCCCGTCCAA CTGAGAAAAC CATTTTCATC GTCTTCATGC TGGTAGTGTC TTTGGTGTCT 
CTTGCCTTGA ACATCATTGA GCTTTTCTAC GTGTTCTTCA AGGGTGTCAA GGATCGTGTG 
AAAGGAAAAA CCGACCCCTA CTCCCACAGT GGCACGATGA GCCCTTCCAA GGACTGTGGT 
TCCCCCAAAT ATGCGTATTA CAATGGCTGT TCCTCACCGA CCGCCCCCTT GTCTCCCATG 
TCTCCCCCGG GGTACAAGCT TGTTACCGGA GACAGGAACA ATTCCTCCTG TCGTAACTAC 
AATAAGCAAG CCAGCGAGCA AAACTGGGCC AACTACAGTG CGGAGCAGAA TAGGATGGGG 
CAGGCTGGCA GCACCATCTC CAACTCTCAT GCCCAGCCCT TCGACTTCGC CGATGAGCAC 
CAGAACACGA AAAAACTGGC GTCGGGACAC GAGCTGCAAC CCCTCACCAT TGTGGACCAG 
AGGCCTCCCA GCAGAGCCAG CAGCCGAGCC AGCAGCAGGC CTCGACCTGA TGACCTGGAG 
ATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989917.1
CDS	180..1325
Translation	MGDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESAWGDEQSAFRCNTQQPGCENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLAHVFYVMRKEEKLNKREEELKVVQNDGVNVDMHLKQIESKKFKYGIEEHGKVKMRGGLLRTYIISILFKSVFEVAFLLIQWYIYGFSLSAIYTCERDPCPHRVDCFLSRPTEKTIFIVFMLVVSLVSLALNIIELFYVFFKGVKDRVKGKTDPYSHSGTMSPSKDCGSPKYAYYNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFADEHQNTKKLASGHELQPLTIVDQRPPSRASSRASSRPRPDDLEI

Target ORF information:

RefSeq Version	NM_204586.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus gap junction protein alpha 1 (GJA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204586.2

ORF Insert Sequence:

ATGGGTGATT GGAGTGCCCT GGGAAAACTT CTTGACAAGG TTCAAGCTTA TTCTACTGCA 
GGAGGAAAAG TGTGGCTGTC TGTCCTCTTT ATTTTCCGAA TCCTGCTATT GGGGACAGCA 
GTCGAGTCTG CTTGGGGAGA TGAACAGTCT GCTTTTCGGT GCAATACTCA ACAGCCTGGT 
TGTGAGAACG TCTGCTATGA CAAGTCCTTC CCCATCTCCC ACGTGCGTTT CTGGGTTCTG 
CAGATCATAT TTGTGTCTGT ACCAACCCTC TTGTACCTGG CGCACGTGTT CTACGTGATG 
AGGAAAGAAG AGAAGCTGAA CAAAAGAGAG GAAGAGCTCA AGGTGGTCCA GAATGATGGT 
GTGAATGTGG ATATGCACCT CAAACAAATA GAAAGTAAGA AATTCAAGTA TGGAATTGAA 
GAGCACGGCA AGGTAAAGAT GCGTGGAGGA CTGCTTCGTA CTTACATCAT CAGCATCCTG 
TTTAAGTCTG TCTTCGAGGT GGCTTTCCTG CTGATACAGT GGTACATTTA TGGGTTTAGC 
CTGAGTGCCA TCTACACCTG TGAGCGAGAC CCATGCCCTC ACAGGGTGGA CTGTTTCCTG 
TCCCGTCCAA CTGAGAAAAC CATTTTCATC GTCTTCATGC TGGTAGTGTC TTTGGTGTCT 
CTTGCCTTGA ACATCATTGA GCTTTTCTAC GTGTTCTTCA AGGGTGTCAA GGATCGTGTG 
AAAGGAAAAA CCGACCCCTA CTCCCACAGT GGCACGATGA GCCCTTCCAA GGACTGTGGT 
TCCCCCAAAT ATGCGTATTA CAATGGCTGT TCCTCACCGA CCGCCCCCTT GTCTCCCATG 
TCTCCCCCGG GGTACAAGCT TGTTACCGGA GACAGGAACA ATTCCTCCTG TCGTAACTAC 
AATAAGCAAG CCAGCGAGCA AAACTGGGCC AACTACAGTG CGGAGCAGAA TAGGATGGGG 
CAGGCTGGCA GCACCATCTC CAACTCTCAT GCCCAGCCCT TCGACTTCGC CGATGAGCAC 
CAGAACACGA AAAAACTGGC GTCGGGACAC GAGCTGCAAC CCCTCACCAT TGTGGACCAG 
AGGCCTCCCA GCAGAGCCAG CAGCCGAGCC AGCAGCAGGC CTCGACCTGA TGACCTGGAG 
ATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Polymorphisms in GJA1 and their association with growth traits in chicken.
Genetics and molecular research : GMR14(4)18839-50(2015 Dec)
Shahjahan M,Liu RR,Zhao GP,Zhang JJ,Zheng MQ,Li QH,Wen J

Adaptation of connexin 43-hemichannel prostaglandin release to mechanical loading.
The Journal of biological chemistry283(39)26374-82(2008 Sep)
Siller-Jackson AJ,Burra S,Gu S,Xia X,Bonewald LF,Sprague E,Jiang JX

Hormonal, pH, and calcium regulation of connexin 43-mediated dye transfer in osteocytes in chick calvaria.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research23(3)350-60(2008 Mar)
Ishihara Y,Kamioka H,Honjo T,Ueda H,Takano-Yamamoto T,Yamashiro T

Connexin 43 is localized with actin in tenocytes.
Cell motility and the cytoskeleton64(2)121-30(2007 Feb)
Wall ME,Otey C,Qi J,Banes AJ

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GJA1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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