NDC80 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	NDC80
Entrez Gene ID	395134
Full Name	NDC80, kinetochore complex component
Synonyms	HEC1,KNTC2
General protein information	Preferred Names NDC80, kinetochore complex component Names kinetochore protein NDC80 homolog NDC80 homolog, kinetochore complex component NDC80 kinetochore complex component homolog kinetochore associated 2 kinetochore protein Hec1 kinetochore-associated protein 2
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015277933.1	XP_015133419.1	kinetochore protein NDC80 homolog isoform X1
XM_015277933.2	XP_015133419.1	kinetochore protein NDC80 homolog isoform X1
XM_025147012.1	XP_025002780.1	kinetochore protein NDC80 homolog isoform X1
NM_204477.2	NP_989808.2	kinetochore protein NDC80 homolog

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NDC80	XP_001146732.1
Bos taurus (cattle)	NDC80	NP_001193512.1
Mus musculus (house mouse)	Ndc80	NP_075783.2
	NDC80	XP_001086615.1
Gallus gallus (chicken)	NDC80	NP_989808.2
Danio rerio (zebrafish)	ndc80	NP_001003863.1
Canis lupus familiaris (dog)	NDC80	XP_537313.2
Oryza sativa (rice)	Os08g0468400	NP_001062008.1
Xenopus tropicalis (tropical clawed frog)	ndc80	NP_001011162.1
Arabidopsis thaliana (thale cress)	AT3G54630	NP_191024.1
Homo sapiens (human)	NDC80	NP_006092.1
Rattus norvegicus (Norway rat)	Ndc80	NP_001119742.1

Gene Ontology
Bibliography

Related articles in PubMed

CENP-A is required for accurate chromosome segregation and sustained kinetochore association of BubR1.
R?gnier V, Vagnarelli P, Fukagawa T, Zerjal T, Burns E, Trouche D, Earnshaw W, Brown W
Molecular and cellular biology25(10)3967-81(2005 May)

The functional region of CENP-H interacts with the Nuf2 complex that localizes to centromere during mitosis.
Mikami Y, Hori T, Kimura H, Fukagawa T
Molecular and cellular biology25(5)1958-70(2005 Mar)

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Caldwell RB, Kierzek AM, Arakawa H, Bezzubov Y, Zaim J, Fiedler P, Kutter S, Blagodatski A, Kostovska D, Koter M, Plachy J, Carninci P, Hayashizaki Y, Buerstedde JM
Genome biology6(1)R6(2005)

Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cells.
Hori T, Haraguchi T, Hiraoka Y, Kimura H, Fukagawa T
Journal of cell science116(Pt 16)3347-62(2003 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NDC80 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NDC80 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa26731	NM_204477.2 Latest version!	Gallus gallus NDC80, kinetochore complex component (NDC80), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OGa32477	XM_015277933.2 Latest version!	Gallus gallus NDC80, kinetochore complex component (NDC80), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OGa32477	XM_025147012.1 Latest version!	Gallus gallus NDC80, kinetochore complex component (NDC80), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OGa32477	XM_015277933.1	Gallus gallus NDC80 kinetochore complex component (NDC80), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NDC80 ( NM_204477.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_989808.2 Gallus gallus NDC80, kinetochore complex component (NDC80), mRNA.

Price & Availability↑

CloneID	OGa26731
Clone ID Related Accession (Same CDS sequence)	NM_204477.2
Accession Version	NM_204477.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-14
Organism	Gallus gallus(chicken)
Product	kinetochore protein NDC80 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ719646.1. On Oct 10, 2004 this sequence version replaced NM_204477.1. ##Evidence-Data-START## Transcript exon combination :: AJ719646.1, AB098622.1 [ECO:0000332] ##Evidence-Data-END##

ATGAGGCGAA GCTCAACCAC TTCTGGGAGT AGCGGTCGGC AGTCTACAAT GGTACTGAGA 
GTGCAGGACA GCAACAAGAT GGGTCTTCAA ACACCTCAAA CGAAGGACAG AGGAACGTTT 
GGAAAGCTGA GCATGAGCAA AACTACGTCT GCAACTTCAG AAAGAAAAGT CAGCTTTTTT 
GGGAAGAGAG CTAGTGGGGC TGGAGGTTCA CGCAACAGTC AGTATGGCAT GTTTGGTACA 
GAAAAGATCA AGGATCCCAG GCCGCTTCAT GACAAGACGT TCATCCAACA ATGTATCAAA 
CAGCTTTGTG AGTTTCTTGT TGAGAATGGT TATGCCCATA ATGTCACCAT AAAATCACTC 
CAGTCTCCAT CTGTAAAAGA CTTCATAAAA ATCTTCACTT TTATCTATGG ATTTCTCTGC 
CCTTCTTATG AACTGCCTGA CTCAAAATTT GAAGAGGAAA TTCCCAGAGT TTTCAAAGAG 
CTTGGGTACC CTTTTCCTTT GTCAAAAAGC TCTATGTACA CAGTGGGAGC ACCACACACC 
TGGCCTCAGA TTGTGGCAGC TTTGATTTGG TTAACTGACT GTTTCAAGTT GTATGCTGCC 
ATGAGGGAAA ATCCATCTTC ATTTGATGAT GGGCAGAACT GGGGAGGAGA AACGGATGAT 
GGAATTGTAC ATAACAAGCT TTTCATGGAC TACGTTGTAA AGTGCTATGA GCACTTCATG 
AAAGGGGGAG ATACTTACGA AGAATTGGAT GCAGAAGTGC AGTCAAAATT AAAAGATTTA 
TTTAATGTAG ATGAATTCAA AATAGAAGGC TTAGCAGCAG AAAACAAAAG ACTTCACGAA 
GAAATTGCAA GACTAGAAAA AGAAAGGGAA AGTGAGCCGG ATCGTTTGGT ATCGCTACGA 
AAGCTGAGAT CTTCTTTTCA AGCAGATGTT CAGAAATACC AGGCTTATCT GGCTAATCTG 
GAATCTCACA CAACCATCCT TGATCAGAAA ATGAAAAGTG TTAATGAAGA AGTTGAGACA 
ACAGAAATGG AAGTAGAAGC AATGAAGCAG GAGAATGCAC GGCTCCAGCA TATCTTTGAT 
AACCAAAAGT ACTCAGTTGC GGATATCGAG AGAATAAATC ACGAGAGAAA TGAATTGCAG 
CAAACCATTA ATAAGCTAAC TAAGGAACTC GAAGCAGAAG AACATCAGCT GTGGAACGAA 
GAGCTAAAAT ATGCTAGAAA TAAAGAGGCG ATTGAAACAC AGCTGGCAGA GTATCATAAA 
CTGGCTCGAA AGCTGAAATT AATTCCAATA AGTGCTGAGA ATTCTAAAGG GCACGACTTT 
GAGATTCACT TTAATCCTGA GGCAGGACCA AATTGCCTAG TCAAATACAG AACTCAGATT 
AAGGCTCCTC TTATGGAGAT TGTAAACCAG ACTGAGGAAG AAATTAGGAA AGCTACTCAG 
CGGAAAATGA GTTTGGAGAA CACCTTGGAA CAGGTGAATG CAATGGTAGC GGAGAAGAAA 
AGCAGTGTGA AAACACTAAA AGAAGAAGCA GAAAAGCTGG ATGATCTTTA CCATCAGAAG 
CTTAAGGAGG CAGAAGAAGA GGAGCAAAAA TGTGCAAATG AGCTGGAATT GTTAGAGAAG 
CATAAACAGT TACTTGAAAG TGGAGTCAAT GAGGGTCTCA GTGAAGCCAC AAATGAACTG 
CATGATATTC AGAGACAATA CCAAATTGTT ATGCAAACAA CAACAGAAGA GAGCAGGAAA 
GCAGGTGATA ACTTGAATCG TCTCTTAGAA GTGATTACTA CTCATGTAGT ATCTATAGAG 
AAATACCTTG ATGAGCAGAA TTCAAAAATT GACAGAGACT ATGAAGAATT CATGTCTGAA 
GATCTTCTGT CAACCTTGAC CGGAATTCTA GACAGTTATA AAAAGAAGGC TGAAAGCATA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989808.2
CDS	216..2138
Translation	MRRSSTTSGSSGRQSTMVLRVQDSNKMGLQTPQTKDRGTFGKLSMSKTTSATSERKVSFFGKRASGAGGSRNSQYGMFGTEKIKDPRPLHDKTFIQQCIKQLCEFLVENGYAHNVTIKSLQSPSVKDFIKIFTFIYGFLCPSYELPDSKFEEEIPRVFKELGYPFPLSKSSMYTVGAPHTWPQIVAALIWLTDCFKLYAAMRENPSSFDDGQNWGGETDDGIVHNKLFMDYVVKCYEHFMKGGDTYEELDAEVQSKLKDLFNVDEFKIEGLAAENKRLHEEIARLEKERESEPDRLVSLRKLRSSFQADVQKYQAYLANLESHTTILDQKMKSVNEEVETTEMEVEAMKQENARLQHIFDNQKYSVADIERINHERNELQQTINKLTKELEAEEHQLWNEELKYARNKEAIETQLAEYHKLARKLKLIPISAENSKGHDFEIHFNPEAGPNCLVKYRTQIKAPLMEIVNQTEEEIRKATQRKMSLENTLEQVNAMVAEKKSSVKTLKEEAEKLDDLYHQKLKEAEEEEQKCANELELLEKHKQLLESGVNEGLSEATNELHDIQRQYQIVMQTTTEESRKAGDNLNRLLEVITTHVVSIEKYLDEQNSKIDRDYEEFMSEDLLSTLTGILDSYKKKAESI

Target ORF information:

RefSeq Version	NM_204477.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NDC80, kinetochore complex component (NDC80), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204477.2

ORF Insert Sequence:

ATGAGGCGAA GCTCAACCAC TTCTGGGAGT AGCGGTCGGC AGTCTACAAT GGTACTGAGA 
GTGCAGGACA GCAACAAGAT GGGTCTTCAA ACACCTCAAA CGAAGGACAG AGGAACGTTT 
GGAAAGCTGA GCATGAGCAA AACTACGTCT GCAACTTCAG AAAGAAAAGT CAGCTTTTTT 
GGGAAGAGAG CTAGTGGGGC TGGAGGTTCA CGCAACAGTC AGTATGGCAT GTTTGGTACA 
GAAAAGATCA AGGATCCCAG GCCGCTTCAT GACAAGACGT TCATCCAACA ATGTATCAAA 
CAGCTTTGTG AGTTTCTTGT TGAGAATGGT TATGCCCATA ATGTCACCAT AAAATCACTC 
CAGTCTCCAT CTGTAAAAGA CTTCATAAAA ATCTTCACTT TTATCTATGG ATTTCTCTGC 
CCTTCTTATG AACTGCCTGA CTCAAAATTT GAAGAGGAAA TTCCCAGAGT TTTCAAAGAG 
CTTGGGTACC CTTTTCCTTT GTCAAAAAGC TCTATGTACA CAGTGGGAGC ACCACACACC 
TGGCCTCAGA TTGTGGCAGC TTTGATTTGG TTAACTGACT GTTTCAAGTT GTATGCTGCC 
ATGAGGGAAA ATCCATCTTC ATTTGATGAT GGGCAGAACT GGGGAGGAGA AACGGATGAT 
GGAATTGTAC ATAACAAGCT TTTCATGGAC TACGTTGTAA AGTGCTATGA GCACTTCATG 
AAAGGGGGAG ATACTTACGA AGAATTGGAT GCAGAAGTGC AGTCAAAATT AAAAGATTTA 
TTTAATGTAG ATGAATTCAA AATAGAAGGC TTAGCAGCAG AAAACAAAAG ACTTCACGAA 
GAAATTGCAA GACTAGAAAA AGAAAGGGAA AGTGAGCCGG ATCGTTTGGT ATCGCTACGA 
AAGCTGAGAT CTTCTTTTCA AGCAGATGTT CAGAAATACC AGGCTTATCT GGCTAATCTG 
GAATCTCACA CAACCATCCT TGATCAGAAA ATGAAAAGTG TTAATGAAGA AGTTGAGACA 
ACAGAAATGG AAGTAGAAGC AATGAAGCAG GAGAATGCAC GGCTCCAGCA TATCTTTGAT 
AACCAAAAGT ACTCAGTTGC GGATATCGAG AGAATAAATC ACGAGAGAAA TGAATTGCAG 
CAAACCATTA ATAAGCTAAC TAAGGAACTC GAAGCAGAAG AACATCAGCT GTGGAACGAA 
GAGCTAAAAT ATGCTAGAAA TAAAGAGGCG ATTGAAACAC AGCTGGCAGA GTATCATAAA 
CTGGCTCGAA AGCTGAAATT AATTCCAATA AGTGCTGAGA ATTCTAAAGG GCACGACTTT 
GAGATTCACT TTAATCCTGA GGCAGGACCA AATTGCCTAG TCAAATACAG AACTCAGATT 
AAGGCTCCTC TTATGGAGAT TGTAAACCAG ACTGAGGAAG AAATTAGGAA AGCTACTCAG 
CGGAAAATGA GTTTGGAGAA CACCTTGGAA CAGGTGAATG CAATGGTAGC GGAGAAGAAA 
AGCAGTGTGA AAACACTAAA AGAAGAAGCA GAAAAGCTGG ATGATCTTTA CCATCAGAAG 
CTTAAGGAGG CAGAAGAAGA GGAGCAAAAA TGTGCAAATG AGCTGGAATT GTTAGAGAAG 
CATAAACAGT TACTTGAAAG TGGAGTCAAT GAGGGTCTCA GTGAAGCCAC AAATGAACTG 
CATGATATTC AGAGACAATA CCAAATTGTT ATGCAAACAA CAACAGAAGA GAGCAGGAAA 
GCAGGTGATA ACTTGAATCG TCTCTTAGAA GTGATTACTA CTCATGTAGT ATCTATAGAG 
AAATACCTTG ATGAGCAGAA TTCAAAAATT GACAGAGACT ATGAAGAATT CATGTCTGAA 
GATCTTCTGT CAACCTTGAC CGGAATTCTA GACAGTTATA AAAAGAAGGC TGAAAGCATA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDC80 ( XM_015277933.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133419.1 Gallus gallus NDC80 kinetochore complex component (NDC80), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa32477
Clone ID Related Accession (Same CDS sequence)	XM_015277933.1 , XM_015277933.2 , XM_025147012.1
Accession Version	XM_015277933.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	kinetochore protein NDC80 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCGAA GCTCAACCAC TTCTGGGAGT AGCGGTCGGC AGTCTACAAT GGTACTGAGA 
GTGCAGGACA GCAACAAGAT GGGTCTTCAA ACGCCTCAAA CGAAGGACAG AGGAACGTTT 
GGAAAGCTGA GCATGAGCAA AACTACATCT GCAACTTCAG AAAGAAAAGT CAGCTTTTTT 
GGGAAGAGAG CTAGTGGGGC TGGAGGTTCA CGCAACAGTC AGTATGGTAT GTTTGGTACA 
GAAAAGATCA AGGATCCCAG GCCGCTTCAT GACAAGGCGT TCATCCAACA ATGTATCAAA 
CAACTTTGTG AGTTTCTTGT TGAGAATGGT TATGCCCATA ATGTCACCAT AAAATCACTC 
CAGTCTCCAT CTGTAAAAGA CTTCATAAAA ATCTTCACTT TTATCTATGG ATTTCTCTGC 
CCTTCTTATG AACTGCCTGA CTCAAAATTT GAAGAGGAAA TTCCCAGAGT TTTCAAAGAG 
CTTGGGTACC CTTTTCCTTT GTCAAAAAGC TCCATGTACA CAGTGGGAGC ACCACACACC 
TGGCCTCAGA TTGTGGCAGC TTTGATTTGG TTAACTGACT GTTTCAAGTT GTATGCTGCC 
ATGAGGGAAA ATCCATCTTC ATTTGATGAT GGGCAGAACT GGGGAGGAGA AACGGATGAT 
GGAATTGTAC ATAACAAGCT TTTCATGGAC TACGTTGTAA AGTGCTATGA GCACTTCATG 
AAAGGGGGAG ATACTTACGA AGAATTGGAT GCAGAAGTGC AGTCAAAATT AAAAGATTTA 
TTTAATGTAG ATGAATTCAA AATAGAAGGC TTAGCAGCAG AAAACAAAAG ACTTCATGAA 
GAAATTGCAA GACTAGAAAA AGAAAGGGAA AGCGAGCCGG ATCGTTTGGT ATCGCTACGA 
AAGCTGAGAT CTTCTTTTCA AGCAGATGTT CAGAAATACC AGGCTTATCT GGCTAATCTG 
GAATCTCACA CAACCATCCT TGATCAGAAA ATGAAAAGTG TTAATGAAGA AGTTGAGACA 
ACAGAAATGG AAGTAGAAGC AATGAAGCAG GAGAATGCAC GGCTCCAGCA TATCTTTGAT 
AACCAAAAGT ACTCAGTTGC GGATATCGAG AGAATAAATC ACGAGAGAAA TGAGTTGCAG 
CAAACCATTA ATAAGCTAAC TAAGGAACTC GAAGCAGAAG AACATCAGCT GTGGAACGAA 
GAGCTAAAAT ATGCTAGAAA TAAAGAGGCG ATTGAAACAC AGCTGGCAGA GTATCATAAA 
CTGGCTCGAA AGCTGAAATT AATTCCAATA AGTGCTGAGA ATTCTAAAGG GCACGACTTT 
GAGATTCACT TTAATCCTGA GGCAGGACCA AATTGCCTAG TCAAATACAG AACTCAGATT 
AAGGCTCCTC TTATGGAGAT TGTAAACCAG ACTGAGGAAG AAATTAGGAA AGCTACTCAA 
CGGAAAATGA GTTTGGAGGA CACCTTGGAA CAGGTGAATG CAATGGTAGC GGAGAAGAAA 
AGCAGTGTGA AAACACTAAA AGAAGAAGCA GAAAAGCTGG ATGATCTTTA CCATCAGAAG 
CTTAAGGAGG CAGAAGAAGA GGAGCAAAAA TGTGCAAATG AGCTGGAATT GTTAGAGAAG 
CATAAACAGT TACTTGAAAG TGGAGTCAAT GAGGGTCTCA GTGAAGCCAC AAATGAACTA 
CATGATATTC AGAGACAATA CCAAATTGTT ATGCAAACAA CAACAGAAGA GAGCAGGAAA 
GCAGGTGATA ACTTGAATCG TCTCTTAGAA GTGATCACTA CTCATGTAGT ATCTATAGAG 
AAATACCTTG ATGAACAGAA TTCAAAAATT GACAGAGACT ATGAAGAATT CATGTCTGAA 
GATCTTCTGT CAACCTTGAC CGGAATTCTA GACAGTTATA AAAAGAAGGC TGAAAGCATA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133419.1
CDS	164..2086
Translation	MRRSSTTSGSSGRQSTMVLRVQDSNKMGLQTPQTKDRGTFGKLSMSKTTSATSERKVSFFGKRASGAGGSRNSQYGMFGTEKIKDPRPLHDKAFIQQCIKQLCEFLVENGYAHNVTIKSLQSPSVKDFIKIFTFIYGFLCPSYELPDSKFEEEIPRVFKELGYPFPLSKSSMYTVGAPHTWPQIVAALIWLTDCFKLYAAMRENPSSFDDGQNWGGETDDGIVHNKLFMDYVVKCYEHFMKGGDTYEELDAEVQSKLKDLFNVDEFKIEGLAAENKRLHEEIARLEKERESEPDRLVSLRKLRSSFQADVQKYQAYLANLESHTTILDQKMKSVNEEVETTEMEVEAMKQENARLQHIFDNQKYSVADIERINHERNELQQTINKLTKELEAEEHQLWNEELKYARNKEAIETQLAEYHKLARKLKLIPISAENSKGHDFEIHFNPEAGPNCLVKYRTQIKAPLMEIVNQTEEEIRKATQRKMSLEDTLEQVNAMVAEKKSSVKTLKEEAEKLDDLYHQKLKEAEEEEQKCANELELLEKHKQLLESGVNEGLSEATNELHDIQRQYQIVMQTTTEESRKAGDNLNRLLEVITTHVVSIEKYLDEQNSKIDRDYEEFMSEDLLSTLTGILDSYKKKAESI

Target ORF information:

RefSeq Version	XM_015277933.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NDC80 kinetochore complex component (NDC80), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277933.1

ORF Insert Sequence:

ATGAGGCGAA GCTCAACCAC TTCTGGGAGT AGCGGTCGGC AGTCTACAAT GGTACTGAGA 
GTGCAGGACA GCAACAAGAT GGGTCTTCAA ACGCCTCAAA CGAAGGACAG AGGAACGTTT 
GGAAAGCTGA GCATGAGCAA AACTACATCT GCAACTTCAG AAAGAAAAGT CAGCTTTTTT 
GGGAAGAGAG CTAGTGGGGC TGGAGGTTCA CGCAACAGTC AGTATGGTAT GTTTGGTACA 
GAAAAGATCA AGGATCCCAG GCCGCTTCAT GACAAGGCGT TCATCCAACA ATGTATCAAA 
CAACTTTGTG AGTTTCTTGT TGAGAATGGT TATGCCCATA ATGTCACCAT AAAATCACTC 
CAGTCTCCAT CTGTAAAAGA CTTCATAAAA ATCTTCACTT TTATCTATGG ATTTCTCTGC 
CCTTCTTATG AACTGCCTGA CTCAAAATTT GAAGAGGAAA TTCCCAGAGT TTTCAAAGAG 
CTTGGGTACC CTTTTCCTTT GTCAAAAAGC TCCATGTACA CAGTGGGAGC ACCACACACC 
TGGCCTCAGA TTGTGGCAGC TTTGATTTGG TTAACTGACT GTTTCAAGTT GTATGCTGCC 
ATGAGGGAAA ATCCATCTTC ATTTGATGAT GGGCAGAACT GGGGAGGAGA AACGGATGAT 
GGAATTGTAC ATAACAAGCT TTTCATGGAC TACGTTGTAA AGTGCTATGA GCACTTCATG 
AAAGGGGGAG ATACTTACGA AGAATTGGAT GCAGAAGTGC AGTCAAAATT AAAAGATTTA 
TTTAATGTAG ATGAATTCAA AATAGAAGGC TTAGCAGCAG AAAACAAAAG ACTTCATGAA 
GAAATTGCAA GACTAGAAAA AGAAAGGGAA AGCGAGCCGG ATCGTTTGGT ATCGCTACGA 
AAGCTGAGAT CTTCTTTTCA AGCAGATGTT CAGAAATACC AGGCTTATCT GGCTAATCTG 
GAATCTCACA CAACCATCCT TGATCAGAAA ATGAAAAGTG TTAATGAAGA AGTTGAGACA 
ACAGAAATGG AAGTAGAAGC AATGAAGCAG GAGAATGCAC GGCTCCAGCA TATCTTTGAT 
AACCAAAAGT ACTCAGTTGC GGATATCGAG AGAATAAATC ACGAGAGAAA TGAGTTGCAG 
CAAACCATTA ATAAGCTAAC TAAGGAACTC GAAGCAGAAG AACATCAGCT GTGGAACGAA 
GAGCTAAAAT ATGCTAGAAA TAAAGAGGCG ATTGAAACAC AGCTGGCAGA GTATCATAAA 
CTGGCTCGAA AGCTGAAATT AATTCCAATA AGTGCTGAGA ATTCTAAAGG GCACGACTTT 
GAGATTCACT TTAATCCTGA GGCAGGACCA AATTGCCTAG TCAAATACAG AACTCAGATT 
AAGGCTCCTC TTATGGAGAT TGTAAACCAG ACTGAGGAAG AAATTAGGAA AGCTACTCAA 
CGGAAAATGA GTTTGGAGGA CACCTTGGAA CAGGTGAATG CAATGGTAGC GGAGAAGAAA 
AGCAGTGTGA AAACACTAAA AGAAGAAGCA GAAAAGCTGG ATGATCTTTA CCATCAGAAG 
CTTAAGGAGG CAGAAGAAGA GGAGCAAAAA TGTGCAAATG AGCTGGAATT GTTAGAGAAG 
CATAAACAGT TACTTGAAAG TGGAGTCAAT GAGGGTCTCA GTGAAGCCAC AAATGAACTA 
CATGATATTC AGAGACAATA CCAAATTGTT ATGCAAACAA CAACAGAAGA GAGCAGGAAA 
GCAGGTGATA ACTTGAATCG TCTCTTAGAA GTGATCACTA CTCATGTAGT ATCTATAGAG 
AAATACCTTG ATGAACAGAA TTCAAAAATT GACAGAGACT ATGAAGAATT CATGTCTGAA 
GATCTTCTGT CAACCTTGAC CGGAATTCTA GACAGTTATA AAAAGAAGGC TGAAAGCATA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDC80 ( XM_015277933.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133419.1 Gallus gallus NDC80, kinetochore complex component (NDC80), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa32477
Clone ID Related Accession (Same CDS sequence)	XM_015277933.1 , XM_015277933.2 , XM_025147012.1
Accession Version	XM_015277933.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	kinetochore protein NDC80 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277933.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCGAA GCTCAACCAC TTCTGGGAGT AGCGGTCGGC AGTCTACAAT GGTACTGAGA 
GTGCAGGACA GCAACAAGAT GGGTCTTCAA ACGCCTCAAA CGAAGGACAG AGGAACGTTT 
GGAAAGCTGA GCATGAGCAA AACTACATCT GCAACTTCAG AAAGAAAAGT CAGCTTTTTT 
GGGAAGAGAG CTAGTGGGGC TGGAGGTTCA CGCAACAGTC AGTATGGTAT GTTTGGTACA 
GAAAAGATCA AGGATCCCAG GCCGCTTCAT GACAAGGCGT TCATCCAACA ATGTATCAAA 
CAACTTTGTG AGTTTCTTGT TGAGAATGGT TATGCCCATA ATGTCACCAT AAAATCACTC 
CAGTCTCCAT CTGTAAAAGA CTTCATAAAA ATCTTCACTT TTATCTATGG ATTTCTCTGC 
CCTTCTTATG AACTGCCTGA CTCAAAATTT GAAGAGGAAA TTCCCAGAGT TTTCAAAGAG 
CTTGGGTACC CTTTTCCTTT GTCAAAAAGC TCCATGTACA CAGTGGGAGC ACCACACACC 
TGGCCTCAGA TTGTGGCAGC TTTGATTTGG TTAACTGACT GTTTCAAGTT GTATGCTGCC 
ATGAGGGAAA ATCCATCTTC ATTTGATGAT GGGCAGAACT GGGGAGGAGA AACGGATGAT 
GGAATTGTAC ATAACAAGCT TTTCATGGAC TACGTTGTAA AGTGCTATGA GCACTTCATG 
AAAGGGGGAG ATACTTACGA AGAATTGGAT GCAGAAGTGC AGTCAAAATT AAAAGATTTA 
TTTAATGTAG ATGAATTCAA AATAGAAGGC TTAGCAGCAG AAAACAAAAG ACTTCATGAA 
GAAATTGCAA GACTAGAAAA AGAAAGGGAA AGCGAGCCGG ATCGTTTGGT ATCGCTACGA 
AAGCTGAGAT CTTCTTTTCA AGCAGATGTT CAGAAATACC AGGCTTATCT GGCTAATCTG 
GAATCTCACA CAACCATCCT TGATCAGAAA ATGAAAAGTG TTAATGAAGA AGTTGAGACA 
ACAGAAATGG AAGTAGAAGC AATGAAGCAG GAGAATGCAC GGCTCCAGCA TATCTTTGAT 
AACCAAAAGT ACTCAGTTGC GGATATCGAG AGAATAAATC ACGAGAGAAA TGAGTTGCAG 
CAAACCATTA ATAAGCTAAC TAAGGAACTC GAAGCAGAAG AACATCAGCT GTGGAACGAA 
GAGCTAAAAT ATGCTAGAAA TAAAGAGGCG ATTGAAACAC AGCTGGCAGA GTATCATAAA 
CTGGCTCGAA AGCTGAAATT AATTCCAATA AGTGCTGAGA ATTCTAAAGG GCACGACTTT 
GAGATTCACT TTAATCCTGA GGCAGGACCA AATTGCCTAG TCAAATACAG AACTCAGATT 
AAGGCTCCTC TTATGGAGAT TGTAAACCAG ACTGAGGAAG AAATTAGGAA AGCTACTCAA 
CGGAAAATGA GTTTGGAGGA CACCTTGGAA CAGGTGAATG CAATGGTAGC GGAGAAGAAA 
AGCAGTGTGA AAACACTAAA AGAAGAAGCA GAAAAGCTGG ATGATCTTTA CCATCAGAAG 
CTTAAGGAGG CAGAAGAAGA GGAGCAAAAA TGTGCAAATG AGCTGGAATT GTTAGAGAAG 
CATAAACAGT TACTTGAAAG TGGAGTCAAT GAGGGTCTCA GTGAAGCCAC AAATGAACTA 
CATGATATTC AGAGACAATA CCAAATTGTT ATGCAAACAA CAACAGAAGA GAGCAGGAAA 
GCAGGTGATA ACTTGAATCG TCTCTTAGAA GTGATCACTA CTCATGTAGT ATCTATAGAG 
AAATACCTTG ATGAACAGAA TTCAAAAATT GACAGAGACT ATGAAGAATT CATGTCTGAA 
GATCTTCTGT CAACCTTGAC CGGAATTCTA GACAGTTATA AAAAGAAGGC TGAAAGCATA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133419.1
CDS	175..2097
Translation	MRRSSTTSGSSGRQSTMVLRVQDSNKMGLQTPQTKDRGTFGKLSMSKTTSATSERKVSFFGKRASGAGGSRNSQYGMFGTEKIKDPRPLHDKAFIQQCIKQLCEFLVENGYAHNVTIKSLQSPSVKDFIKIFTFIYGFLCPSYELPDSKFEEEIPRVFKELGYPFPLSKSSMYTVGAPHTWPQIVAALIWLTDCFKLYAAMRENPSSFDDGQNWGGETDDGIVHNKLFMDYVVKCYEHFMKGGDTYEELDAEVQSKLKDLFNVDEFKIEGLAAENKRLHEEIARLEKERESEPDRLVSLRKLRSSFQADVQKYQAYLANLESHTTILDQKMKSVNEEVETTEMEVEAMKQENARLQHIFDNQKYSVADIERINHERNELQQTINKLTKELEAEEHQLWNEELKYARNKEAIETQLAEYHKLARKLKLIPISAENSKGHDFEIHFNPEAGPNCLVKYRTQIKAPLMEIVNQTEEEIRKATQRKMSLEDTLEQVNAMVAEKKSSVKTLKEEAEKLDDLYHQKLKEAEEEEQKCANELELLEKHKQLLESGVNEGLSEATNELHDIQRQYQIVMQTTTEESRKAGDNLNRLLEVITTHVVSIEKYLDEQNSKIDRDYEEFMSEDLLSTLTGILDSYKKKAESI

Target ORF information:

RefSeq Version	XM_015277933.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NDC80, kinetochore complex component (NDC80), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277933.2

ORF Insert Sequence:

ATGAGGCGAA GCTCAACCAC TTCTGGGAGT AGCGGTCGGC AGTCTACAAT GGTACTGAGA 
GTGCAGGACA GCAACAAGAT GGGTCTTCAA ACGCCTCAAA CGAAGGACAG AGGAACGTTT 
GGAAAGCTGA GCATGAGCAA AACTACATCT GCAACTTCAG AAAGAAAAGT CAGCTTTTTT 
GGGAAGAGAG CTAGTGGGGC TGGAGGTTCA CGCAACAGTC AGTATGGTAT GTTTGGTACA 
GAAAAGATCA AGGATCCCAG GCCGCTTCAT GACAAGGCGT TCATCCAACA ATGTATCAAA 
CAACTTTGTG AGTTTCTTGT TGAGAATGGT TATGCCCATA ATGTCACCAT AAAATCACTC 
CAGTCTCCAT CTGTAAAAGA CTTCATAAAA ATCTTCACTT TTATCTATGG ATTTCTCTGC 
CCTTCTTATG AACTGCCTGA CTCAAAATTT GAAGAGGAAA TTCCCAGAGT TTTCAAAGAG 
CTTGGGTACC CTTTTCCTTT GTCAAAAAGC TCCATGTACA CAGTGGGAGC ACCACACACC 
TGGCCTCAGA TTGTGGCAGC TTTGATTTGG TTAACTGACT GTTTCAAGTT GTATGCTGCC 
ATGAGGGAAA ATCCATCTTC ATTTGATGAT GGGCAGAACT GGGGAGGAGA AACGGATGAT 
GGAATTGTAC ATAACAAGCT TTTCATGGAC TACGTTGTAA AGTGCTATGA GCACTTCATG 
AAAGGGGGAG ATACTTACGA AGAATTGGAT GCAGAAGTGC AGTCAAAATT AAAAGATTTA 
TTTAATGTAG ATGAATTCAA AATAGAAGGC TTAGCAGCAG AAAACAAAAG ACTTCATGAA 
GAAATTGCAA GACTAGAAAA AGAAAGGGAA AGCGAGCCGG ATCGTTTGGT ATCGCTACGA 
AAGCTGAGAT CTTCTTTTCA AGCAGATGTT CAGAAATACC AGGCTTATCT GGCTAATCTG 
GAATCTCACA CAACCATCCT TGATCAGAAA ATGAAAAGTG TTAATGAAGA AGTTGAGACA 
ACAGAAATGG AAGTAGAAGC AATGAAGCAG GAGAATGCAC GGCTCCAGCA TATCTTTGAT 
AACCAAAAGT ACTCAGTTGC GGATATCGAG AGAATAAATC ACGAGAGAAA TGAGTTGCAG 
CAAACCATTA ATAAGCTAAC TAAGGAACTC GAAGCAGAAG AACATCAGCT GTGGAACGAA 
GAGCTAAAAT ATGCTAGAAA TAAAGAGGCG ATTGAAACAC AGCTGGCAGA GTATCATAAA 
CTGGCTCGAA AGCTGAAATT AATTCCAATA AGTGCTGAGA ATTCTAAAGG GCACGACTTT 
GAGATTCACT TTAATCCTGA GGCAGGACCA AATTGCCTAG TCAAATACAG AACTCAGATT 
AAGGCTCCTC TTATGGAGAT TGTAAACCAG ACTGAGGAAG AAATTAGGAA AGCTACTCAA 
CGGAAAATGA GTTTGGAGGA CACCTTGGAA CAGGTGAATG CAATGGTAGC GGAGAAGAAA 
AGCAGTGTGA AAACACTAAA AGAAGAAGCA GAAAAGCTGG ATGATCTTTA CCATCAGAAG 
CTTAAGGAGG CAGAAGAAGA GGAGCAAAAA TGTGCAAATG AGCTGGAATT GTTAGAGAAG 
CATAAACAGT TACTTGAAAG TGGAGTCAAT GAGGGTCTCA GTGAAGCCAC AAATGAACTA 
CATGATATTC AGAGACAATA CCAAATTGTT ATGCAAACAA CAACAGAAGA GAGCAGGAAA 
GCAGGTGATA ACTTGAATCG TCTCTTAGAA GTGATCACTA CTCATGTAGT ATCTATAGAG 
AAATACCTTG ATGAACAGAA TTCAAAAATT GACAGAGACT ATGAAGAATT CATGTCTGAA 
GATCTTCTGT CAACCTTGAC CGGAATTCTA GACAGTTATA AAAAGAAGGC TGAAAGCATA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDC80 ( XM_025147012.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025002780.1 Gallus gallus NDC80, kinetochore complex component (NDC80), transcript variant X2, mRNA.

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CloneID	OGa32477
Clone ID Related Accession (Same CDS sequence)	XM_015277933.1 , XM_015277933.2 , XM_025147012.1
Accession Version	XM_025147012.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	kinetochore protein NDC80 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCGAA GCTCAACCAC TTCTGGGAGT AGCGGTCGGC AGTCTACAAT GGTACTGAGA 
GTGCAGGACA GCAACAAGAT GGGTCTTCAA ACGCCTCAAA CGAAGGACAG AGGAACGTTT 
GGAAAGCTGA GCATGAGCAA AACTACATCT GCAACTTCAG AAAGAAAAGT CAGCTTTTTT 
GGGAAGAGAG CTAGTGGGGC TGGAGGTTCA CGCAACAGTC AGTATGGTAT GTTTGGTACA 
GAAAAGATCA AGGATCCCAG GCCGCTTCAT GACAAGGCGT TCATCCAACA ATGTATCAAA 
CAACTTTGTG AGTTTCTTGT TGAGAATGGT TATGCCCATA ATGTCACCAT AAAATCACTC 
CAGTCTCCAT CTGTAAAAGA CTTCATAAAA ATCTTCACTT TTATCTATGG ATTTCTCTGC 
CCTTCTTATG AACTGCCTGA CTCAAAATTT GAAGAGGAAA TTCCCAGAGT TTTCAAAGAG 
CTTGGGTACC CTTTTCCTTT GTCAAAAAGC TCCATGTACA CAGTGGGAGC ACCACACACC 
TGGCCTCAGA TTGTGGCAGC TTTGATTTGG TTAACTGACT GTTTCAAGTT GTATGCTGCC 
ATGAGGGAAA ATCCATCTTC ATTTGATGAT GGGCAGAACT GGGGAGGAGA AACGGATGAT 
GGAATTGTAC ATAACAAGCT TTTCATGGAC TACGTTGTAA AGTGCTATGA GCACTTCATG 
AAAGGGGGAG ATACTTACGA AGAATTGGAT GCAGAAGTGC AGTCAAAATT AAAAGATTTA 
TTTAATGTAG ATGAATTCAA AATAGAAGGC TTAGCAGCAG AAAACAAAAG ACTTCATGAA 
GAAATTGCAA GACTAGAAAA AGAAAGGGAA AGCGAGCCGG ATCGTTTGGT ATCGCTACGA 
AAGCTGAGAT CTTCTTTTCA AGCAGATGTT CAGAAATACC AGGCTTATCT GGCTAATCTG 
GAATCTCACA CAACCATCCT TGATCAGAAA ATGAAAAGTG TTAATGAAGA AGTTGAGACA 
ACAGAAATGG AAGTAGAAGC AATGAAGCAG GAGAATGCAC GGCTCCAGCA TATCTTTGAT 
AACCAAAAGT ACTCAGTTGC GGATATCGAG AGAATAAATC ACGAGAGAAA TGAGTTGCAG 
CAAACCATTA ATAAGCTAAC TAAGGAACTC GAAGCAGAAG AACATCAGCT GTGGAACGAA 
GAGCTAAAAT ATGCTAGAAA TAAAGAGGCG ATTGAAACAC AGCTGGCAGA GTATCATAAA 
CTGGCTCGAA AGCTGAAATT AATTCCAATA AGTGCTGAGA ATTCTAAAGG GCACGACTTT 
GAGATTCACT TTAATCCTGA GGCAGGACCA AATTGCCTAG TCAAATACAG AACTCAGATT 
AAGGCTCCTC TTATGGAGAT TGTAAACCAG ACTGAGGAAG AAATTAGGAA AGCTACTCAA 
CGGAAAATGA GTTTGGAGGA CACCTTGGAA CAGGTGAATG CAATGGTAGC GGAGAAGAAA 
AGCAGTGTGA AAACACTAAA AGAAGAAGCA GAAAAGCTGG ATGATCTTTA CCATCAGAAG 
CTTAAGGAGG CAGAAGAAGA GGAGCAAAAA TGTGCAAATG AGCTGGAATT GTTAGAGAAG 
CATAAACAGT TACTTGAAAG TGGAGTCAAT GAGGGTCTCA GTGAAGCCAC AAATGAACTA 
CATGATATTC AGAGACAATA CCAAATTGTT ATGCAAACAA CAACAGAAGA GAGCAGGAAA 
GCAGGTGATA ACTTGAATCG TCTCTTAGAA GTGATCACTA CTCATGTAGT ATCTATAGAG 
AAATACCTTG ATGAACAGAA TTCAAAAATT GACAGAGACT ATGAAGAATT CATGTCTGAA 
GATCTTCTGT CAACCTTGAC CGGAATTCTA GACAGTTATA AAAAGAAGGC TGAAAGCATA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025002780.1
CDS	186..2108
Translation	MRRSSTTSGSSGRQSTMVLRVQDSNKMGLQTPQTKDRGTFGKLSMSKTTSATSERKVSFFGKRASGAGGSRNSQYGMFGTEKIKDPRPLHDKAFIQQCIKQLCEFLVENGYAHNVTIKSLQSPSVKDFIKIFTFIYGFLCPSYELPDSKFEEEIPRVFKELGYPFPLSKSSMYTVGAPHTWPQIVAALIWLTDCFKLYAAMRENPSSFDDGQNWGGETDDGIVHNKLFMDYVVKCYEHFMKGGDTYEELDAEVQSKLKDLFNVDEFKIEGLAAENKRLHEEIARLEKERESEPDRLVSLRKLRSSFQADVQKYQAYLANLESHTTILDQKMKSVNEEVETTEMEVEAMKQENARLQHIFDNQKYSVADIERINHERNELQQTINKLTKELEAEEHQLWNEELKYARNKEAIETQLAEYHKLARKLKLIPISAENSKGHDFEIHFNPEAGPNCLVKYRTQIKAPLMEIVNQTEEEIRKATQRKMSLEDTLEQVNAMVAEKKSSVKTLKEEAEKLDDLYHQKLKEAEEEEQKCANELELLEKHKQLLESGVNEGLSEATNELHDIQRQYQIVMQTTTEESRKAGDNLNRLLEVITTHVVSIEKYLDEQNSKIDRDYEEFMSEDLLSTLTGILDSYKKKAESI

Target ORF information:

RefSeq Version	XM_025147012.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NDC80, kinetochore complex component (NDC80), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025147012.1

ORF Insert Sequence:

ATGAGGCGAA GCTCAACCAC TTCTGGGAGT AGCGGTCGGC AGTCTACAAT GGTACTGAGA 
GTGCAGGACA GCAACAAGAT GGGTCTTCAA ACGCCTCAAA CGAAGGACAG AGGAACGTTT 
GGAAAGCTGA GCATGAGCAA AACTACATCT GCAACTTCAG AAAGAAAAGT CAGCTTTTTT 
GGGAAGAGAG CTAGTGGGGC TGGAGGTTCA CGCAACAGTC AGTATGGTAT GTTTGGTACA 
GAAAAGATCA AGGATCCCAG GCCGCTTCAT GACAAGGCGT TCATCCAACA ATGTATCAAA 
CAACTTTGTG AGTTTCTTGT TGAGAATGGT TATGCCCATA ATGTCACCAT AAAATCACTC 
CAGTCTCCAT CTGTAAAAGA CTTCATAAAA ATCTTCACTT TTATCTATGG ATTTCTCTGC 
CCTTCTTATG AACTGCCTGA CTCAAAATTT GAAGAGGAAA TTCCCAGAGT TTTCAAAGAG 
CTTGGGTACC CTTTTCCTTT GTCAAAAAGC TCCATGTACA CAGTGGGAGC ACCACACACC 
TGGCCTCAGA TTGTGGCAGC TTTGATTTGG TTAACTGACT GTTTCAAGTT GTATGCTGCC 
ATGAGGGAAA ATCCATCTTC ATTTGATGAT GGGCAGAACT GGGGAGGAGA AACGGATGAT 
GGAATTGTAC ATAACAAGCT TTTCATGGAC TACGTTGTAA AGTGCTATGA GCACTTCATG 
AAAGGGGGAG ATACTTACGA AGAATTGGAT GCAGAAGTGC AGTCAAAATT AAAAGATTTA 
TTTAATGTAG ATGAATTCAA AATAGAAGGC TTAGCAGCAG AAAACAAAAG ACTTCATGAA 
GAAATTGCAA GACTAGAAAA AGAAAGGGAA AGCGAGCCGG ATCGTTTGGT ATCGCTACGA 
AAGCTGAGAT CTTCTTTTCA AGCAGATGTT CAGAAATACC AGGCTTATCT GGCTAATCTG 
GAATCTCACA CAACCATCCT TGATCAGAAA ATGAAAAGTG TTAATGAAGA AGTTGAGACA 
ACAGAAATGG AAGTAGAAGC AATGAAGCAG GAGAATGCAC GGCTCCAGCA TATCTTTGAT 
AACCAAAAGT ACTCAGTTGC GGATATCGAG AGAATAAATC ACGAGAGAAA TGAGTTGCAG 
CAAACCATTA ATAAGCTAAC TAAGGAACTC GAAGCAGAAG AACATCAGCT GTGGAACGAA 
GAGCTAAAAT ATGCTAGAAA TAAAGAGGCG ATTGAAACAC AGCTGGCAGA GTATCATAAA 
CTGGCTCGAA AGCTGAAATT AATTCCAATA AGTGCTGAGA ATTCTAAAGG GCACGACTTT 
GAGATTCACT TTAATCCTGA GGCAGGACCA AATTGCCTAG TCAAATACAG AACTCAGATT 
AAGGCTCCTC TTATGGAGAT TGTAAACCAG ACTGAGGAAG AAATTAGGAA AGCTACTCAA 
CGGAAAATGA GTTTGGAGGA CACCTTGGAA CAGGTGAATG CAATGGTAGC GGAGAAGAAA 
AGCAGTGTGA AAACACTAAA AGAAGAAGCA GAAAAGCTGG ATGATCTTTA CCATCAGAAG 
CTTAAGGAGG CAGAAGAAGA GGAGCAAAAA TGTGCAAATG AGCTGGAATT GTTAGAGAAG 
CATAAACAGT TACTTGAAAG TGGAGTCAAT GAGGGTCTCA GTGAAGCCAC AAATGAACTA 
CATGATATTC AGAGACAATA CCAAATTGTT ATGCAAACAA CAACAGAAGA GAGCAGGAAA 
GCAGGTGATA ACTTGAATCG TCTCTTAGAA GTGATCACTA CTCATGTAGT ATCTATAGAG 
AAATACCTTG ATGAACAGAA TTCAAAAATT GACAGAGACT ATGAAGAATT CATGTCTGAA 
GATCTTCTGT CAACCTTGAC CGGAATTCTA GACAGTTATA AAAAGAAGGC TGAAAGCATA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CENP-A is required for accurate chromosome segregation and sustained kinetochore association of BubR1.
Molecular and cellular biology25(10)3967-81(2005 May)
R?gnier V,Vagnarelli P,Fukagawa T,Zerjal T,Burns E,Trouche D,Earnshaw W,Brown W

The functional region of CENP-H interacts with the Nuf2 complex that localizes to centromere during mitosis.
Molecular and cellular biology25(5)1958-70(2005 Mar)
Mikami Y,Hori T,Kimura H,Fukagawa T

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Genome biology6(1)R6(2005)
Caldwell RB,Kierzek AM,Arakawa H,Bezzubov Y,Zaim J,Fiedler P,Kutter S,Blagodatski A,Kostovska D,Koter M,Plachy J,Carninci P,Hayashizaki Y,Buerstedde JM

Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cells.
Journal of cell science116(Pt 16)3347-62(2003 Aug)
Hori T,Haraguchi T,Hiraoka Y,Kimura H,Fukagawa T

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NDC80 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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