COL2A1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL2A1
Entrez Gene ID	395069
Full Name	collagen type II alpha 1 chain
General protein information	Preferred Names collagen type II alpha 1 chain Names collagen alpha-1(II) chain alpha 1 type II procollagen alpha 1 type IIA collagen alpha-1 type II collagen collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia, congenital) procollagen alpha 1(II) type II collagen
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025145274.1	XP_025001042.1	collagen alpha-1(II) chain isoform X1
NM_204426.1	NP_989757.1	collagen alpha-1(II) chain precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	COL2A1	NP_001001135.2
Gallus gallus (chicken)	COL2A1	NP_989757.1
Xenopus tropicalis (tropical clawed frog)	col2a1	NP_989220.1
	COL2A1	XP_001100739.1
Canis lupus familiaris (dog)	COL2A1	NP_001006952.1
Rattus norvegicus (Norway rat)	Col2a1	NP_037061.1
Danio rerio (zebrafish)	col2a1a	NP_571367.1
Pan troglodytes (chimpanzee)	COL2A1	XP_509026.2
Homo sapiens (human)	COL2A1	NP_001835.3
Mus musculus (house mouse)	Col2a1	NP_112440.2

Gene Ontology
Bibliography

Related articles in PubMed

Interaction of lubricin with type II collagen surfaces: adsorption, friction, and normal forces.
Chang DP, Guilak F, Jay GD, Zauscher S
Journal of biomechanics47(3)659-66(2014 Feb)

Molecular cloning, characterization and localization of chicken type II procollagen gene.
Xi C, Liu N, Liang F, Guo S, Sun Y, Yang F, Xi Y
Gene366(1)67-76(2006 Jan)

Structure and sequence of the chicken type II procollagen gene. Characterization of the region encoding the carboxyl-terminal telopeptide and propeptide.
Sandell LJ, Prentice HL, Kravis D, Upholt WB
The Journal of biological chemistry259(12)7826-34(1984 Jun)

Isolation of cDNA and genomic DNA clones encoding type II collagen.
Young MF, Vogeli G, Nunez AM, Fernandez MP, Sullivan M, Sobel ME
Nucleic acids research12(10)4207-28(1984 May)

Identification of genomic DNA coding for chicken type II procollagen.
Sandell LJ, Yamada Y, Dorfman A, Upholt WB
The Journal of biological chemistry258(19)11617-21(1983 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We examined the molecular details by which lubricin interacts with type II collagen and how binding is related to boundary lubrication and adhesive interactions; and whether collagen structure can affect lubricin adsorption.
Title: Interaction of lubricin with type II collagen surfaces: adsorption, friction, and normal forces.

the structural organization, evolutional characters, developmental expression and chromosomal mapping of the gene
Title: Molecular cloning, characterization and localization of chicken type II procollagen gene.

The following COL2A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL2A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa149625	XM_025145274.1 Latest version!	Gallus gallus collagen type II alpha 1 chain (COL2A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa26664	NM_204426.1 Latest version!	Gallus gallus collagen type II alpha 1 chain (COL2A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL2A1 ( XM_025145274.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025001042.1 Gallus gallus collagen type II alpha 1 chain (COL2A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa149625
Clone ID Related Accession (Same CDS sequence)	XM_025145274.1
Accession Version	XM_025145274.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	collagen alpha-1(II) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008465.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGGCC GCCGCCCGCC CCGCTCCGCC GCTCTCCTCC TCCTCCTCCT CCTTCTCACG 
GCCGCCGCAG CCGCGCAGGA CCGCGACCTC CCGGAGGCCG CCGGGGGCTG CGTGCAGGAC 
GGGCAGAGCT ACAGCGATAA GGACGTGTGG AAGCCGGAGC CGTGCCGGAT CTGCGTGTGC 
GACACCGGGA CCGTTCTGTG CGACGAGATC ATCTGCGAGG AGCCGCAGGA CTGCCCCAGC 
CCCGAAATCC CCTTCGGAGA GTGCTGCCCC GTCTGCCCCG CAGAGCTGCC CGCCGCCGCC 
CGACAACCTG GCCCCAAGGG ACAGAAGGGA GAACCCGGAG ATATTAAAGA TGTTGTAGGA 
CCCCGAGGGC CTCCAGGACC ACAGGGCCCA GCAGGAGAGC AGGGACAGCG AGGGGACCGT 
GGCGAGAAGG GGGAGAAGGG TGCTCCTGGC CCCCGTGGAA GGGATGGAGA ACCCGGCACC 
CCTGGAAACC CAGGCCCCCC CGGTCCCCCC GGACCTCCTG GCCCCCCCGG ACTTGGTGGA 
AACTTTGCGG CGCAGATGGC GGGCGGCTTC GATGAGAAGG CGGGTGGAGC GCAGATGGGT 
GTCATGCAGG GACCCATGGG CCCTATGGGA CCCCGCGGCC CCCCTGGCCC CACTGGCGCA 
CCTGGTCCCC AGGGATTTCA AGGCAACCCC GGTGAGCCCG GCGAACCCGG CGCTGCTGGT 
CCGATGGGTC CCCGGGGACC TCCGGGACCA CCTGGGAAAC CCGGTGACGA TGGTGAGACA 
GGCAAACCCG GCAAATCTGG TGAACGTGGC CCCCCCGGCC CCCAGGGCGC TCGTGGCTTC 
CCTGGGACTC CTGGTCTCCC CGGAGTGAAA GGCCACCGAG GCTACCCCGG TTTGGATGGT 
GCCAAAGGAG AGGCGGGGGC TCCTGGAGCC AAGGGTGAAT CTGGTTCGCC GGGTGAGAAC 
GGCTCCCCCG GCCCCATGGG ACCCCGTGGG CTGCCCGGAG AGCGAGGACG TCCCGGCCCC 
TCCGGCGCCG CCGGTGCTCG TGGCAATGAC GGTCTCCCTG GCCCTGCTGG ACCCCCTGGA 
CCCGTCGGCC CTGCCGGAGC CCCCGGCTTC CCCGGAGCCC CCGGTTCAAA GGGTGAAGCC 
GGCCCCACTG GCGCACGGGG TCCCGAGGGT GCCCAAGGAC CCCGCGGCGA ATCCGGCACC 
CCCGGCTCTC CCGGCCCCGC TGGCGCACCC GGTAACCCAG GGACTGATGG CATCCCCGGT 
GCCAAGGGCT CGGCGGGTGC CCCGGGCATT GCAGGCGCTC CAGGATTCCC CGGCCCACGC 
GGCCCCCCCG GACCCCAAGG TGCCACCGGA CCACTGGGAC CCAAAGGACA GACGGGCGAA 
CCCGGCATCG CAGGCTTCAA GGGCGAGCAA GGACCGAAGG GCGAGACGGG CCCCGCAGGA 
CCCCAAGGTG CCCCCGGGCC GGCTGGCGAG GAAGGCAAGA GAGGAGCTCG TGGTGAACCT 
GGTGCCGCCG GCCCTGTGGG CCCCCCCGGA GAAAGGGGCG CTCCTGGCAA CCGTGGATTC 
CCCGGGCAGG ACGGGCTGGC CGGACCCAAG GGTGCTCCAG GTGAACGCGG CCCCGCTGGT 
CTTGCCGGTC CCAAAGGTGC CACCGGTGAC CCCGGACGTC CCGGAGAGCC CGGGCTGCCC 
GGAGCGAGGG GTCTCACCGG CCGCCCTGGC GATGCGGGAC CTCAAGGCAA AGTCGGCCCA 
ACTGGTGCTC CTGGCGAGGA TGGCCGCCCC GGCCCCCCCG GCCCTCAGGG TGCTCGTGGG 
CAGCCCGGTG TGATGGGTTT CCCCGGTCCC AAAGGCGCTA ATGGTGAGCC TGGAAAAGCT 
GGAGAGAAAG GACTGCCCGG CGCTCCAGGG CTGCGGGGTC TGCCTGGCAA GGATGGGGAG 
ACGGGAGCTG CCGGCCCCCC TGGACCCGCT GGTCCTGTGG GTGAGAGAGG AGAGCAAGGA 
GCCCCCGGTC CTTCCGGCTT CCAGGGACTG CCCGGACCAC CAGGTCCCCC TGGGGAGAGC 
GGCAAACCCG GAGACCAGGG TGTTCCTGGA GAAGCCGGTG CCCCCGGTCT TGTTGGTCCC 
AGAGGTGAAC GTGGATTCCC CGGTGAACGC GGCTCTCCCG GTGCCCAAGG GCTGCAGGGT 
CCCCGTGGGC TCCCCGGAAC GCCCGGCACT GACGGACCCA AGGGTGCAAC CGGTCCAGCC 
GGCCCCAACG GTGCCCAGGG TCCCCCAGGG CTGCAGGGAA TGCCCGGTGA GAGAGGAGCA 
GCTGGCATCG CTGGCCCCAA GGGTGACCGG GGAGATGTTG GTGAGAAAGG ACCTGAGGGA 
GCTCCAGGCA AGGATGGCGC ACGTGGTCTG ACGGGTCCCA TTGGTCCCCC TGGCCCTGCT 
GGCCCCAACG GTGAGAAGGG TGAATCCGGC CCTCCTGGTC CATCTGGTGC TGCCGGTGCC 
CGTGGTGCCC CCGGTGAGCG TGGCGAGCCC GGTGCCCCCG GTCCTGCTGG ATTTGCTGGC 
CCCCCGGGCG CCGATGGACA ACCCGGTGCC AAAGGCGAGC AGGGAGAGCC CGGGCAGAAG 
GGTGACGCGG GCGCTCCTGG TCCCCAAGGT CCCTCCGGCG CTCCTGGCCC CCAGGGTCCA 
ACCGGTGTCA CTGGTCCCAA AGGAGCTCGT GGGGCTCAGG GTCCCCCTGG AGCCACGGGA 
TTCCCCGGAG CTGCCGGCCG TGTGGGACCG CCCGGCCCTA ATGGTAACCC AGGCCCCCCC 
GGACCCCCTG GCTCTGCTGG CAAAGACGGC CCCAAGGGTG TTCGTGGCGA CGCCGGCCCC 
CCCGGCCGTG CAGGTGACCC CGGCCTCCAA GGCCCCGCCG GCCCCCCCGG CGAGAAGGGC 
GAACCCGGCG AGGACGGCCC CGCGGGTCCC GACGGCCCCC CCGGCCCTCA AGGCTTGGCA 
GGACAGCGTG GTATTGTGGG TCTCCCAGGA CAGCGTGGTG AGAGAGGCTT CCCCGGACTG 
CCGGGGCCAT CGGGAGAACC TGGAAAGCAA GGAGCGCCTG GCTCTGCGGG TGACCGAGGT 
CCCCCCGGCC CCGTGGGGCC CCCTGGGCTG ACAGGTCCTG CTGGAGAACC CGGGCGCGAG 
GGCAACCCTG GTGCTGACGG TCCCCCAGGC AGGGATGGCG CAGCTGGCGT GAAGGGTGAT 
CGTGGTGAGA CCGGCCCTGT GGGTGCCCCC GGTGCTCCTG GAGCCCCTGG CGCCCCCGGC 
CCTGTTGGTC CCACTGGAAA ACAAGGAGAC AGAGGCGAGA CGGGTGCACA AGGGCCCATG 
GGTCCCTCTG GTCCCGCTGG AGCTCGAGGA ATGCCGGGTC CCCAAGGACC TCGTGGTGAC 
AAAGGTGAGA CGGGAGAGGC TGGAGAGAGA GGGCTGAAGG GCCACCGTGG CTTCACCGGT 
CTGCAGGGTC TGCCCGGACC ACCCGGCCCG TCTGGAGACC AAGGTGCTGC CGGTCCCGCT 
GGTCCCTCCG GTCCCAGAGG TCCCCCTGGT CCCGTCGGCC CCTCTGGCAA AGATGGCTCT 
AACGGCATGC CCGGCCCCAT CGGTCCTCCC GGTCCCCGTG GACGGAGTGG TGAACCCGGC 
CCTGCGGGTC CTCCTGGAAA CCCCGGTCCT CCCGGTCCTC CTGGCCCCCC CGGCACCGGC 
ATCGACATGT CTGCTTTTGC TGGACTGGGT CAGACGGAGA AGGGCCCCGA CCCCATCCGC 
TACATGAGGG CAGACGAGGC GGCCGGAGGG CTGCGGCAGC ACGACGTGGA GGTGGATGCC 
ACCCTCAAAT CCCTCAACAA TCAGATTGAG AGCATCCGCA GCCCCGAGGG CTCCAAGAAG 
AACCCTGCCA GGACCTGCCG CGACATCAAA CTCTGCCATC CCGAGTGGAA GAGCGGAGAT 
TACTGGATTG ACCCGAACCA GGGCTGCACC TTGGACGCCA TCAAAGTATT CTGCAACATG 
GAGACGGGCG AGACCTGCGT CTACCCGACC CCCAGCAGCA TCCCCAGGAA GAACTGGTGG 
ACCAGCAAGA CGAAAGACAA GAAGCACGTC TGGTTTGCAG AGACCATCAA CGGCGGTTTC 
CACTTCAGCT ACGGCGATGA GAACCTGTCC CCCAACACCG CCAGCATCCA GATGACCTTC 
CTGCGCCTCC TGTCCACCGA GGGCTCCCAG AACGTCACCT ACCACTGCAA GAACAGCATC 
GCCTACATGG ACGAGGAGAC GGGCAACCTG AAGAAAGCCA TCCTCATCCA GGGATCCAAC 
GACGTGGAGA TCAGAGCCGA GGGCAACAGC AGGTTCACCT ACAGCGTCTT GGAGGACGGC 
TGCACGAAAC ACACTGGCAA ATGGGGCAAG ACGGTGATCG AGTACCGGTC GCAGAAGACC 
TCGCGCCTGC CCATTGTAGA TATTGCACCT ATGGACATTG GCGGAGCCGA TCAGGAGTTT 
GGCGTGGATA TTGGCCCAGT CTGCTTCTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025001042.1
CDS	67..4539
Translation	MHGRRPPRSAALLLLLLLLTAAAAAQDRDLPEAAGGCVQDGQSYSDKDVWKPEPCRICVCDTGTVLCDEIICEEPQDCPSPEIPFGECCPVCPAELPAAARQPGPKGQKGEPGDIKDVVGPRGPPGPQGPAGEQGQRGDRGEKGEKGAPGPRGRDGEPGTPGNPGPPGPPGPPGPPGLGGNFAAQMAGGFDEKAGGAQMGVMQGPMGPMGPRGPPGPTGAPGPQGFQGNPGEPGEPGAAGPMGPRGPPGPPGKPGDDGETGKPGKSGERGPPGPQGARGFPGTPGLPGVKGHRGYPGLDGAKGEAGAPGAKGESGSPGENGSPGPMGPRGLPGERGRPGPSGAAGARGNDGLPGPAGPPGPVGPAGAPGFPGAPGSKGEAGPTGARGPEGAQGPRGESGTPGSPGPAGAPGNPGTDGIPGAKGSAGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQTGEPGIAGFKGEQGPKGETGPAGPQGAPGPAGEEGKRGARGEPGAAGPVGPPGERGAPGNRGFPGQDGLAGPKGAPGERGPAGLAGPKGATGDPGRPGEPGLPGARGLTGRPGDAGPQGKVGPTGAPGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLPGAPGLRGLPGKDGETGAAGPPGPAGPVGERGEQGAPGPSGFQGLPGPPGPPGESGKPGDQGVPGEAGAPGLVGPRGERGFPGERGSPGAQGLQGPRGLPGTPGTDGPKGATGPAGPNGAQGPPGLQGMPGERGAAGIAGPKGDRGDVGEKGPEGAPGKDGARGLTGPIGPPGPAGPNGEKGESGPPGPSGAAGARGAPGERGEPGAPGPAGFAGPPGADGQPGAKGEQGEPGQKGDAGAPGPQGPSGAPGPQGPTGVTGPKGARGAQGPPGATGFPGAAGRVGPPGPNGNPGPPGPPGSAGKDGPKGVRGDAGPPGRAGDPGLQGPAGPPGEKGEPGEDGPAGPDGPPGPQGLAGQRGIVGLPGQRGERGFPGLPGPSGEPGKQGAPGSAGDRGPPGPVGPPGLTGPAGEPGREGNPGADGPPGRDGAAGVKGDRGETGPVGAPGAPGAPGAPGPVGPTGKQGDRGETGAQGPMGPSGPAGARGMPGPQGPRGDKGETGEAGERGLKGHRGFTGLQGLPGPPGPSGDQGAAGPAGPSGPRGPPGPVGPSGKDGSNGMPGPIGPPGPRGRSGEPGPAGPPGNPGPPGPPGPPGTGIDMSAFAGLGQTEKGPDPIRYMRADEAAGGLRQHDVEVDATLKSLNNQIESIRSPEGSKKNPARTCRDIKLCHPEWKSGDYWIDPNQGCTLDAIKVFCNMETGETCVYPTPSSIPRKNWWTSKTKDKKHVWFAETINGGFHFSYGDENLSPNTASIQMTFLRLLSTEGSQNVTYHCKNSIAYMDEETGNLKKAILIQGSNDVEIRAEGNSRFTYSVLEDGCTKHTGKWGKTVIEYRSQKTSRLPIVDIAPMDIGGADQEFGVDIGPVCFL

Target ORF information:

RefSeq Version	XM_025145274.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen type II alpha 1 chain (COL2A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025145274.1

ORF Insert Sequence:

ATGCACGGCC GCCGCCCGCC CCGCTCCGCC GCTCTCCTCC TCCTCCTCCT CCTTCTCACG 
GCCGCCGCAG CCGCGCAGGA CCGCGACCTC CCGGAGGCCG CCGGGGGCTG CGTGCAGGAC 
GGGCAGAGCT ACAGCGATAA GGACGTGTGG AAGCCGGAGC CGTGCCGGAT CTGCGTGTGC 
GACACCGGGA CCGTTCTGTG CGACGAGATC ATCTGCGAGG AGCCGCAGGA CTGCCCCAGC 
CCCGAAATCC CCTTCGGAGA GTGCTGCCCC GTCTGCCCCG CAGAGCTGCC CGCCGCCGCC 
CGACAACCTG GCCCCAAGGG ACAGAAGGGA GAACCCGGAG ATATTAAAGA TGTTGTAGGA 
CCCCGAGGGC CTCCAGGACC ACAGGGCCCA GCAGGAGAGC AGGGACAGCG AGGGGACCGT 
GGCGAGAAGG GGGAGAAGGG TGCTCCTGGC CCCCGTGGAA GGGATGGAGA ACCCGGCACC 
CCTGGAAACC CAGGCCCCCC CGGTCCCCCC GGACCTCCTG GCCCCCCCGG ACTTGGTGGA 
AACTTTGCGG CGCAGATGGC GGGCGGCTTC GATGAGAAGG CGGGTGGAGC GCAGATGGGT 
GTCATGCAGG GACCCATGGG CCCTATGGGA CCCCGCGGCC CCCCTGGCCC CACTGGCGCA 
CCTGGTCCCC AGGGATTTCA AGGCAACCCC GGTGAGCCCG GCGAACCCGG CGCTGCTGGT 
CCGATGGGTC CCCGGGGACC TCCGGGACCA CCTGGGAAAC CCGGTGACGA TGGTGAGACA 
GGCAAACCCG GCAAATCTGG TGAACGTGGC CCCCCCGGCC CCCAGGGCGC TCGTGGCTTC 
CCTGGGACTC CTGGTCTCCC CGGAGTGAAA GGCCACCGAG GCTACCCCGG TTTGGATGGT 
GCCAAAGGAG AGGCGGGGGC TCCTGGAGCC AAGGGTGAAT CTGGTTCGCC GGGTGAGAAC 
GGCTCCCCCG GCCCCATGGG ACCCCGTGGG CTGCCCGGAG AGCGAGGACG TCCCGGCCCC 
TCCGGCGCCG CCGGTGCTCG TGGCAATGAC GGTCTCCCTG GCCCTGCTGG ACCCCCTGGA 
CCCGTCGGCC CTGCCGGAGC CCCCGGCTTC CCCGGAGCCC CCGGTTCAAA GGGTGAAGCC 
GGCCCCACTG GCGCACGGGG TCCCGAGGGT GCCCAAGGAC CCCGCGGCGA ATCCGGCACC 
CCCGGCTCTC CCGGCCCCGC TGGCGCACCC GGTAACCCAG GGACTGATGG CATCCCCGGT 
GCCAAGGGCT CGGCGGGTGC CCCGGGCATT GCAGGCGCTC CAGGATTCCC CGGCCCACGC 
GGCCCCCCCG GACCCCAAGG TGCCACCGGA CCACTGGGAC CCAAAGGACA GACGGGCGAA 
CCCGGCATCG CAGGCTTCAA GGGCGAGCAA GGACCGAAGG GCGAGACGGG CCCCGCAGGA 
CCCCAAGGTG CCCCCGGGCC GGCTGGCGAG GAAGGCAAGA GAGGAGCTCG TGGTGAACCT 
GGTGCCGCCG GCCCTGTGGG CCCCCCCGGA GAAAGGGGCG CTCCTGGCAA CCGTGGATTC 
CCCGGGCAGG ACGGGCTGGC CGGACCCAAG GGTGCTCCAG GTGAACGCGG CCCCGCTGGT 
CTTGCCGGTC CCAAAGGTGC CACCGGTGAC CCCGGACGTC CCGGAGAGCC CGGGCTGCCC 
GGAGCGAGGG GTCTCACCGG CCGCCCTGGC GATGCGGGAC CTCAAGGCAA AGTCGGCCCA 
ACTGGTGCTC CTGGCGAGGA TGGCCGCCCC GGCCCCCCCG GCCCTCAGGG TGCTCGTGGG 
CAGCCCGGTG TGATGGGTTT CCCCGGTCCC AAAGGCGCTA ATGGTGAGCC TGGAAAAGCT 
GGAGAGAAAG GACTGCCCGG CGCTCCAGGG CTGCGGGGTC TGCCTGGCAA GGATGGGGAG 
ACGGGAGCTG CCGGCCCCCC TGGACCCGCT GGTCCTGTGG GTGAGAGAGG AGAGCAAGGA 
GCCCCCGGTC CTTCCGGCTT CCAGGGACTG CCCGGACCAC CAGGTCCCCC TGGGGAGAGC 
GGCAAACCCG GAGACCAGGG TGTTCCTGGA GAAGCCGGTG CCCCCGGTCT TGTTGGTCCC 
AGAGGTGAAC GTGGATTCCC CGGTGAACGC GGCTCTCCCG GTGCCCAAGG GCTGCAGGGT 
CCCCGTGGGC TCCCCGGAAC GCCCGGCACT GACGGACCCA AGGGTGCAAC CGGTCCAGCC 
GGCCCCAACG GTGCCCAGGG TCCCCCAGGG CTGCAGGGAA TGCCCGGTGA GAGAGGAGCA 
GCTGGCATCG CTGGCCCCAA GGGTGACCGG GGAGATGTTG GTGAGAAAGG ACCTGAGGGA 
GCTCCAGGCA AGGATGGCGC ACGTGGTCTG ACGGGTCCCA TTGGTCCCCC TGGCCCTGCT 
GGCCCCAACG GTGAGAAGGG TGAATCCGGC CCTCCTGGTC CATCTGGTGC TGCCGGTGCC 
CGTGGTGCCC CCGGTGAGCG TGGCGAGCCC GGTGCCCCCG GTCCTGCTGG ATTTGCTGGC 
CCCCCGGGCG CCGATGGACA ACCCGGTGCC AAAGGCGAGC AGGGAGAGCC CGGGCAGAAG 
GGTGACGCGG GCGCTCCTGG TCCCCAAGGT CCCTCCGGCG CTCCTGGCCC CCAGGGTCCA 
ACCGGTGTCA CTGGTCCCAA AGGAGCTCGT GGGGCTCAGG GTCCCCCTGG AGCCACGGGA 
TTCCCCGGAG CTGCCGGCCG TGTGGGACCG CCCGGCCCTA ATGGTAACCC AGGCCCCCCC 
GGACCCCCTG GCTCTGCTGG CAAAGACGGC CCCAAGGGTG TTCGTGGCGA CGCCGGCCCC 
CCCGGCCGTG CAGGTGACCC CGGCCTCCAA GGCCCCGCCG GCCCCCCCGG CGAGAAGGGC 
GAACCCGGCG AGGACGGCCC CGCGGGTCCC GACGGCCCCC CCGGCCCTCA AGGCTTGGCA 
GGACAGCGTG GTATTGTGGG TCTCCCAGGA CAGCGTGGTG AGAGAGGCTT CCCCGGACTG 
CCGGGGCCAT CGGGAGAACC TGGAAAGCAA GGAGCGCCTG GCTCTGCGGG TGACCGAGGT 
CCCCCCGGCC CCGTGGGGCC CCCTGGGCTG ACAGGTCCTG CTGGAGAACC CGGGCGCGAG 
GGCAACCCTG GTGCTGACGG TCCCCCAGGC AGGGATGGCG CAGCTGGCGT GAAGGGTGAT 
CGTGGTGAGA CCGGCCCTGT GGGTGCCCCC GGTGCTCCTG GAGCCCCTGG CGCCCCCGGC 
CCTGTTGGTC CCACTGGAAA ACAAGGAGAC AGAGGCGAGA CGGGTGCACA AGGGCCCATG 
GGTCCCTCTG GTCCCGCTGG AGCTCGAGGA ATGCCGGGTC CCCAAGGACC TCGTGGTGAC 
AAAGGTGAGA CGGGAGAGGC TGGAGAGAGA GGGCTGAAGG GCCACCGTGG CTTCACCGGT 
CTGCAGGGTC TGCCCGGACC ACCCGGCCCG TCTGGAGACC AAGGTGCTGC CGGTCCCGCT 
GGTCCCTCCG GTCCCAGAGG TCCCCCTGGT CCCGTCGGCC CCTCTGGCAA AGATGGCTCT 
AACGGCATGC CCGGCCCCAT CGGTCCTCCC GGTCCCCGTG GACGGAGTGG TGAACCCGGC 
CCTGCGGGTC CTCCTGGAAA CCCCGGTCCT CCCGGTCCTC CTGGCCCCCC CGGCACCGGC 
ATCGACATGT CTGCTTTTGC TGGACTGGGT CAGACGGAGA AGGGCCCCGA CCCCATCCGC 
TACATGAGGG CAGACGAGGC GGCCGGAGGG CTGCGGCAGC ACGACGTGGA GGTGGATGCC 
ACCCTCAAAT CCCTCAACAA TCAGATTGAG AGCATCCGCA GCCCCGAGGG CTCCAAGAAG 
AACCCTGCCA GGACCTGCCG CGACATCAAA CTCTGCCATC CCGAGTGGAA GAGCGGAGAT 
TACTGGATTG ACCCGAACCA GGGCTGCACC TTGGACGCCA TCAAAGTATT CTGCAACATG 
GAGACGGGCG AGACCTGCGT CTACCCGACC CCCAGCAGCA TCCCCAGGAA GAACTGGTGG 
ACCAGCAAGA CGAAAGACAA GAAGCACGTC TGGTTTGCAG AGACCATCAA CGGCGGTTTC 
CACTTCAGCT ACGGCGATGA GAACCTGTCC CCCAACACCG CCAGCATCCA GATGACCTTC 
CTGCGCCTCC TGTCCACCGA GGGCTCCCAG AACGTCACCT ACCACTGCAA GAACAGCATC 
GCCTACATGG ACGAGGAGAC GGGCAACCTG AAGAAAGCCA TCCTCATCCA GGGATCCAAC 
GACGTGGAGA TCAGAGCCGA GGGCAACAGC AGGTTCACCT ACAGCGTCTT GGAGGACGGC 
TGCACGAAAC ACACTGGCAA ATGGGGCAAG ACGGTGATCG AGTACCGGTC GCAGAAGACC 
TCGCGCCTGC CCATTGTAGA TATTGCACCT ATGGACATTG GCGGAGCCGA TCAGGAGTTT 
GGCGTGGATA TTGGCCCAGT CTGCTTCTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL2A1 ( NM_204426.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_989757.1 Gallus gallus collagen type II alpha 1 chain (COL2A1), mRNA.

Price & Availability↑

CloneID	OGa26664
Clone ID Related Accession (Same CDS sequence)	NM_204426.1
Accession Version	NM_204426.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Gallus gallus(chicken)
Product	collagen alpha-1(II) chain precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY046949.1. On Aug 30, 2004 this sequence version replaced XM_423849.1. ##Evidence-Data-START## Transcript exon combination :: AY046949.1 [ECO:0000332] ##Evidence-Data-END##

ATGCACGGCC GCCGCCCGCC CCGCTCCGCC GCTCTCCTCC TCCTCCTCCT CCTTCTCACG 
GCCGCCGCAG CCGCGCAGGA CCGCGACCTC CGACAACCTG GCCCCAAGGG ACAGAAGGGA 
GAACCCGGAG ATATTAAAGA TGTTGTAGGA CCCCGAGGGC CTCCAGGACC ACAGGGCCCA 
GCAGGAGAGC AGGGACAGCG AGGGGACCGT GGCGAGAAGG GGGAGAAGGG TGCTCCTGGC 
CCCCGTGGGA GGGATGGAGA ACCCGGCACC CCTGGAAACC CAGGCCCCCC CGGTCCCCCC 
GGACCTCCTG GCCCCCCCGG ACTTGGTGGA AACTTTGCGG CGCAGATGGC GGGCGGCTTC 
GATGAGAAGG CGGGTGGAGC GCAGATGGGT GTCATGCAGG GACCCATGGG CCCTATGGGA 
CCCCGCGGCC CCCCTGGCCC CACTGGCGCA CCTGGTCCCC AGGGATTTCA AGGCAACCCC 
GGTGAGCCCG GCGAACCCGG CGCTGCTGGT CCGATGGGTC CCCGGGGACC TCCGGGACCA 
CCTGGGAAAC CCGGTGACGA TGGTGAGACA GGCAAACCCG GCAAATCTGG TGAACGTGGC 
CCCCCCGGCC CCCAGGGCGC TCGTGGCTTC CCTGGGACTC CTGGTCTCCC CGGAGTGAAG 
GGCCACCGAG GCTACCCCGG TTTGGATGGT GCCAAAGGAG AGGCGGGGGC TCCTGGAGCC 
AAGGGTGAAT CTGGTTCACC GGGTGAGAAC GGCTCCCCCG GCCCCATGGG ACCCCGTGGG 
CTGCCCGGAG AGCGAGGACG TCCCGGCCCC TCCGGCGCCG CCGGTGCTCG TGGCAATGAC 
GGTCTCCCTG GCCCTGCTGG ACCCCCTGGA CCCGTCGGCC CTGCCGGAGC CCCCGGCTTC 
CCCGGAGCCC CCGGTTCAAA GGGTGAAGCC GGCCCCACTG GTGCACGGGG TCCCGAGGGT 
GCCCAAGGAC CCCGCGGCGA ATCCGGCACC CCCGGCTCTC CCGGCCCCGC TGGCGCACCC 
GGTAACCCAG GGACTGATGG CATCCCCGGT GCCAAGGGCT CGGCGGGTGC CCCGGGCATT 
GCAGGCGCTC CAGGATTCCC CGGCCCACGC GGCCCCCCCG GACCCCAAGG TGCCACCGGA 
CCACTGGGAC CCAAAGGACA GACGGGCGAA CCCGGCATCG CAGGCTTCAA GGGCGAGCAA 
GGACCGAAGG GCGAGACGGG CCCCGCAGGA CCCCAAGGTG CCCCCGGGCC GGCTGGTGAG 
GAAGGCAAGA GAGGAGCTCG TGGTGAACCT GGTGCCGCCG GCCCTGTGGG CCCCCCCGGA 
GAAAGGGGCG CTCCTGGCAA CCGTGGATTC CCCGGGCAGG ACGGGCTGGC CGGACCCAAG 
GGTGCTCCAG GTGAACGCGG CCCCGCTGGT CTCGCCGGTC CCAAAGGTGC CACCGGTGAC 
CCCGGACGTC CCGGAGAGCC CGGGCTGCCC GGAGCGAGGG GTCTCACCGG CCGCCCCGGC 
GATGCGGGAC CTCAAGGCAA AGTCGGCCCA ACTGGTGCTC CTGGCGAGGA TGGCCGCCCC 
GGCCCCCCCG GACCTCAGGG TGCTCGTGGG CAGCCTGGTG TGATGGGTTT CCCCGGTCCC 
AAAGGCGCTA ATGGTGAGCC TGGAAAAGCT GGAGAGAAAG GACTGCCCGG CGCCCCAGGG 
CTGCGGGGTC TGCCTGGCAA GGATGGGGAG ACGGGAGCTG CCGGCCCCCC TGGACCCGCT 
GGTCCTGTGG GTGAGAGAGG AGAGCAAGGA GCCCCCGGTC CTTCCGGCTT CCAGGGACTG 
CCCGGACCAC CAGGTCCCCC TGGGGAGAGC GGCAAACCCG GAGACCAGGG TGTTCCTGGA 
GAAGCCGGTG CCCCCGGTCT TGTTGGTCCC AGAGGTGAAC GTGGATTCCC CGGTGAACGC 
GGCTCTCCCG GTGCCCAAGG GCTGCAGGGT CCCCGTGGGC TCCCCGGAAC GCCCGGCACT 
GACGGACCCA AGGGTGCAAC CGGTCCAGCC GGCCCCAACG GTGCCCAGGG TCCCCCAGGG 
CTGCAGGGAA TGCCCGGTGA GAGAGGAGCA GCTGGCATCG CTGGCCTCAA GGGTGACCGG 
GGAGATGTTG GTGAGAAAGG ACCTGAGGGA GCTCCAGGCA AGGATGGCGC ACGTGGTCTG 
ACGGGTCCCA TTGGTCCCCC TGGCCCTGCT GGCCCCAACG GTGAGAAGGG TGAATCCGGC 
CCTCCTGGTC CATCTGGTGC TGCCGGTGCC CGTGGTGCCC CCGGTGAGCG TGGCGAGCCC 
GGTGCCCCCG GTCCTGCTGG ATTTGCTGGC CCCCCGGGCG CCGATGGACA ACCCGGTGCC 
AAAGGCGAGC AGGGAGAGCC CGGGCAGAAG GGTGACGCGG GCGCTCCTGG TCCCCAAGGT 
CCCTCCGGCG CTCCTGGCCC CCAGGGCCCA ACCGGTGTCA CTGGTCCCAA AGGAGCTCGT 
GGGGCTCAGG GTCCCCCTGG AGCCACGGGA TTCCCCGGAG CTGCCGGCCG TGTGGGACCG 
CCCGGCCCTA ATGGTAACCC AGGCCCCCCC GGACCCCCTG GCTCTGCTGG CAAGGACGGC 
CCCAAGGGTG TTCGTGGCGA CGCCGGCCCC CCCGGCCGTG CAGGTGACCC CGGCCTCCAA 
GGCCCCGCCG GCCCCCCCGG CGAGAAGGGC GAACCCGGCG AGGACGGCCC CGCGGGTCCC 
GACGGCCCCC CCGGCCCTCA AGGCTTGGCA GGACAGCGTG GTATTGTGGG TCTCCCAGGA 
CAGCGTGGTG AGAGAGGCTT CCCCGGACTG CCGGGGCCAT CGGGAGAACC TGGAAAGCAA 
GGAGCGCCTG GCTCTGCGGG TGACCGAGGT CCCCCCGGCC CCGTGGGGCC CCCTGGGCTG 
ACGGGTCCTG CTGGAGAACC CGGGCGCGAG GGCAACCCTG GTGCTGACGG TCTCCCAGGC 
AGGGATGGCG CAGCTGGCGT GAAGGGTGAT CGTGGTGAGA CCGGCCCTGT GGGTGCCCCC 
GGTGCTCCTG GAGCCCCTGG CGCCCCCGGC CCTGTTGGTC CCACTGGAAA ACAAGGAGAC 
AGAGGCGAGA CGGGTGCACA AGGGCCCATG GGTCCCTCTG GTCCCGCTGG AGCTCGAGGA 
ATGCCGGGTC CCCAAGGACC TCGTGGTGAC AAAGGTGAGA CGGGAGAGGC TGGAGAGAGA 
GGGCTGAAGG GCCACCGTGG CTTCACCGGT CTGCAGGGTC TGCCCGGACC ACCCGGCCCG 
TCTGGAGACC AAGGTGCTGC CGGTCCCGCT GGTCCCTCCG GTCCCAGAGG TCCCCCTGGT 
CCCGTCGGCC CCTCTGGCAA AGATGGCTCT AACGGCATGC CCGGCCCCAT CGGTCCTCCC 
GGTCCCCGTG GACGGAGTGG TGAACCCGGC CCTGCGGGTC CTCCTGGAAA CCCCGGTCCT 
CCCGGTCCTC CTGGCCCCCC CGGCACCGGC ATCGACATGT CTGCTTTTGC TGGACTGGGT 
CAGACGGAGA AGGGCCCCGA CCCCATCCGC TACATGAGGG CAGACGAGGC GGCCGGAGGG 
CTGCGGCAGC ACGACGTGGA GGTGGATGCC ACCCTCAAAT CCCTCAACAA TCAGATTGAG 
AGCATCCGCA GCCCCGAGGG CTCCAAGAAG AACCCTGCCA GGACCTGCCG CGACATCAAA 
CTCTGCCATC CCGAGTGGAA GAGCGGAGAT TACTGGATTG ACCCGAACCA GGGCTGCACC 
TTGGACGCCA TCAAAGTATT CTGCAACATG GAGACGGGCG AGACCTGCGT CTACCCGACC 
CCCAGCAGCA TCCCCAGGAA GAACTGGTGG ACCAGCAAGA CGAAAGACAA GAAGCACGTC 
TGGTTTGCAG AGACCATCAA CGGCGGTTTC CACTTCAGCT ACGGCGATGA GAACCTGTCC 
CCCAACACCG CCAGCATCCA GATGACCTTC CTGCGCCTCC TGTCCACCGA GGGCTCCCAG 
AACGTCACCT ACCACTGCAA GAACAGCATC GCCTACATGG ACGAGGAGAC GGGCAACCTG 
AAGAAAGCCA TCCTCATCCA GGGATCCAAC GACGTGGAGA TCAGAGCCGA GGGCAACAGC 
AGGTTCACCT ACAGCGTCTT GGAGGACGGC TGCACGAAAC ACACTGGCAA ATGGGGCAAG 
ACGGTGATCG AGTACCGGTT GCAGAAGACC TCGCGCCTGT CCATTGTAGA TACTGCACCT 
ATGGACATTG GCGGAGCCGA TCAGGAGTTT GGCGTGGATA TTGGCCCAGT CTGCTTCTTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989757.1
CDS	45..4307
Translation	MHGRRPPRSAALLLLLLLLTAAAAAQDRDLRQPGPKGQKGEPGDIKDVVGPRGPPGPQGPAGEQGQRGDRGEKGEKGAPGPRGRDGEPGTPGNPGPPGPPGPPGPPGLGGNFAAQMAGGFDEKAGGAQMGVMQGPMGPMGPRGPPGPTGAPGPQGFQGNPGEPGEPGAAGPMGPRGPPGPPGKPGDDGETGKPGKSGERGPPGPQGARGFPGTPGLPGVKGHRGYPGLDGAKGEAGAPGAKGESGSPGENGSPGPMGPRGLPGERGRPGPSGAAGARGNDGLPGPAGPPGPVGPAGAPGFPGAPGSKGEAGPTGARGPEGAQGPRGESGTPGSPGPAGAPGNPGTDGIPGAKGSAGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQTGEPGIAGFKGEQGPKGETGPAGPQGAPGPAGEEGKRGARGEPGAAGPVGPPGERGAPGNRGFPGQDGLAGPKGAPGERGPAGLAGPKGATGDPGRPGEPGLPGARGLTGRPGDAGPQGKVGPTGAPGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLPGAPGLRGLPGKDGETGAAGPPGPAGPVGERGEQGAPGPSGFQGLPGPPGPPGESGKPGDQGVPGEAGAPGLVGPRGERGFPGERGSPGAQGLQGPRGLPGTPGTDGPKGATGPAGPNGAQGPPGLQGMPGERGAAGIAGLKGDRGDVGEKGPEGAPGKDGARGLTGPIGPPGPAGPNGEKGESGPPGPSGAAGARGAPGERGEPGAPGPAGFAGPPGADGQPGAKGEQGEPGQKGDAGAPGPQGPSGAPGPQGPTGVTGPKGARGAQGPPGATGFPGAAGRVGPPGPNGNPGPPGPPGSAGKDGPKGVRGDAGPPGRAGDPGLQGPAGPPGEKGEPGEDGPAGPDGPPGPQGLAGQRGIVGLPGQRGERGFPGLPGPSGEPGKQGAPGSAGDRGPPGPVGPPGLTGPAGEPGREGNPGADGLPGRDGAAGVKGDRGETGPVGAPGAPGAPGAPGPVGPTGKQGDRGETGAQGPMGPSGPAGARGMPGPQGPRGDKGETGEAGERGLKGHRGFTGLQGLPGPPGPSGDQGAAGPAGPSGPRGPPGPVGPSGKDGSNGMPGPIGPPGPRGRSGEPGPAGPPGNPGPPGPPGPPGTGIDMSAFAGLGQTEKGPDPIRYMRADEAAGGLRQHDVEVDATLKSLNNQIESIRSPEGSKKNPARTCRDIKLCHPEWKSGDYWIDPNQGCTLDAIKVFCNMETGETCVYPTPSSIPRKNWWTSKTKDKKHVWFAETINGGFHFSYGDENLSPNTASIQMTFLRLLSTEGSQNVTYHCKNSIAYMDEETGNLKKAILIQGSNDVEIRAEGNSRFTYSVLEDGCTKHTGKWGKTVIEYRLQKTSRLSIVDTAPMDIGGADQEFGVDIGPVCFL

Target ORF information:

RefSeq Version	NM_204426.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen type II alpha 1 chain (COL2A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204426.1

ORF Insert Sequence:

ATGCACGGCC GCCGCCCGCC CCGCTCCGCC GCTCTCCTCC TCCTCCTCCT CCTTCTCACG 
GCCGCCGCAG CCGCGCAGGA CCGCGACCTC CGACAACCTG GCCCCAAGGG ACAGAAGGGA 
GAACCCGGAG ATATTAAAGA TGTTGTAGGA CCCCGAGGGC CTCCAGGACC ACAGGGCCCA 
GCAGGAGAGC AGGGACAGCG AGGGGACCGT GGCGAGAAGG GGGAGAAGGG TGCTCCTGGC 
CCCCGTGGGA GGGATGGAGA ACCCGGCACC CCTGGAAACC CAGGCCCCCC CGGTCCCCCC 
GGACCTCCTG GCCCCCCCGG ACTTGGTGGA AACTTTGCGG CGCAGATGGC GGGCGGCTTC 
GATGAGAAGG CGGGTGGAGC GCAGATGGGT GTCATGCAGG GACCCATGGG CCCTATGGGA 
CCCCGCGGCC CCCCTGGCCC CACTGGCGCA CCTGGTCCCC AGGGATTTCA AGGCAACCCC 
GGTGAGCCCG GCGAACCCGG CGCTGCTGGT CCGATGGGTC CCCGGGGACC TCCGGGACCA 
CCTGGGAAAC CCGGTGACGA TGGTGAGACA GGCAAACCCG GCAAATCTGG TGAACGTGGC 
CCCCCCGGCC CCCAGGGCGC TCGTGGCTTC CCTGGGACTC CTGGTCTCCC CGGAGTGAAG 
GGCCACCGAG GCTACCCCGG TTTGGATGGT GCCAAAGGAG AGGCGGGGGC TCCTGGAGCC 
AAGGGTGAAT CTGGTTCACC GGGTGAGAAC GGCTCCCCCG GCCCCATGGG ACCCCGTGGG 
CTGCCCGGAG AGCGAGGACG TCCCGGCCCC TCCGGCGCCG CCGGTGCTCG TGGCAATGAC 
GGTCTCCCTG GCCCTGCTGG ACCCCCTGGA CCCGTCGGCC CTGCCGGAGC CCCCGGCTTC 
CCCGGAGCCC CCGGTTCAAA GGGTGAAGCC GGCCCCACTG GTGCACGGGG TCCCGAGGGT 
GCCCAAGGAC CCCGCGGCGA ATCCGGCACC CCCGGCTCTC CCGGCCCCGC TGGCGCACCC 
GGTAACCCAG GGACTGATGG CATCCCCGGT GCCAAGGGCT CGGCGGGTGC CCCGGGCATT 
GCAGGCGCTC CAGGATTCCC CGGCCCACGC GGCCCCCCCG GACCCCAAGG TGCCACCGGA 
CCACTGGGAC CCAAAGGACA GACGGGCGAA CCCGGCATCG CAGGCTTCAA GGGCGAGCAA 
GGACCGAAGG GCGAGACGGG CCCCGCAGGA CCCCAAGGTG CCCCCGGGCC GGCTGGTGAG 
GAAGGCAAGA GAGGAGCTCG TGGTGAACCT GGTGCCGCCG GCCCTGTGGG CCCCCCCGGA 
GAAAGGGGCG CTCCTGGCAA CCGTGGATTC CCCGGGCAGG ACGGGCTGGC CGGACCCAAG 
GGTGCTCCAG GTGAACGCGG CCCCGCTGGT CTCGCCGGTC CCAAAGGTGC CACCGGTGAC 
CCCGGACGTC CCGGAGAGCC CGGGCTGCCC GGAGCGAGGG GTCTCACCGG CCGCCCCGGC 
GATGCGGGAC CTCAAGGCAA AGTCGGCCCA ACTGGTGCTC CTGGCGAGGA TGGCCGCCCC 
GGCCCCCCCG GACCTCAGGG TGCTCGTGGG CAGCCTGGTG TGATGGGTTT CCCCGGTCCC 
AAAGGCGCTA ATGGTGAGCC TGGAAAAGCT GGAGAGAAAG GACTGCCCGG CGCCCCAGGG 
CTGCGGGGTC TGCCTGGCAA GGATGGGGAG ACGGGAGCTG CCGGCCCCCC TGGACCCGCT 
GGTCCTGTGG GTGAGAGAGG AGAGCAAGGA GCCCCCGGTC CTTCCGGCTT CCAGGGACTG 
CCCGGACCAC CAGGTCCCCC TGGGGAGAGC GGCAAACCCG GAGACCAGGG TGTTCCTGGA 
GAAGCCGGTG CCCCCGGTCT TGTTGGTCCC AGAGGTGAAC GTGGATTCCC CGGTGAACGC 
GGCTCTCCCG GTGCCCAAGG GCTGCAGGGT CCCCGTGGGC TCCCCGGAAC GCCCGGCACT 
GACGGACCCA AGGGTGCAAC CGGTCCAGCC GGCCCCAACG GTGCCCAGGG TCCCCCAGGG 
CTGCAGGGAA TGCCCGGTGA GAGAGGAGCA GCTGGCATCG CTGGCCTCAA GGGTGACCGG 
GGAGATGTTG GTGAGAAAGG ACCTGAGGGA GCTCCAGGCA AGGATGGCGC ACGTGGTCTG 
ACGGGTCCCA TTGGTCCCCC TGGCCCTGCT GGCCCCAACG GTGAGAAGGG TGAATCCGGC 
CCTCCTGGTC CATCTGGTGC TGCCGGTGCC CGTGGTGCCC CCGGTGAGCG TGGCGAGCCC 
GGTGCCCCCG GTCCTGCTGG ATTTGCTGGC CCCCCGGGCG CCGATGGACA ACCCGGTGCC 
AAAGGCGAGC AGGGAGAGCC CGGGCAGAAG GGTGACGCGG GCGCTCCTGG TCCCCAAGGT 
CCCTCCGGCG CTCCTGGCCC CCAGGGCCCA ACCGGTGTCA CTGGTCCCAA AGGAGCTCGT 
GGGGCTCAGG GTCCCCCTGG AGCCACGGGA TTCCCCGGAG CTGCCGGCCG TGTGGGACCG 
CCCGGCCCTA ATGGTAACCC AGGCCCCCCC GGACCCCCTG GCTCTGCTGG CAAGGACGGC 
CCCAAGGGTG TTCGTGGCGA CGCCGGCCCC CCCGGCCGTG CAGGTGACCC CGGCCTCCAA 
GGCCCCGCCG GCCCCCCCGG CGAGAAGGGC GAACCCGGCG AGGACGGCCC CGCGGGTCCC 
GACGGCCCCC CCGGCCCTCA AGGCTTGGCA GGACAGCGTG GTATTGTGGG TCTCCCAGGA 
CAGCGTGGTG AGAGAGGCTT CCCCGGACTG CCGGGGCCAT CGGGAGAACC TGGAAAGCAA 
GGAGCGCCTG GCTCTGCGGG TGACCGAGGT CCCCCCGGCC CCGTGGGGCC CCCTGGGCTG 
ACGGGTCCTG CTGGAGAACC CGGGCGCGAG GGCAACCCTG GTGCTGACGG TCTCCCAGGC 
AGGGATGGCG CAGCTGGCGT GAAGGGTGAT CGTGGTGAGA CCGGCCCTGT GGGTGCCCCC 
GGTGCTCCTG GAGCCCCTGG CGCCCCCGGC CCTGTTGGTC CCACTGGAAA ACAAGGAGAC 
AGAGGCGAGA CGGGTGCACA AGGGCCCATG GGTCCCTCTG GTCCCGCTGG AGCTCGAGGA 
ATGCCGGGTC CCCAAGGACC TCGTGGTGAC AAAGGTGAGA CGGGAGAGGC TGGAGAGAGA 
GGGCTGAAGG GCCACCGTGG CTTCACCGGT CTGCAGGGTC TGCCCGGACC ACCCGGCCCG 
TCTGGAGACC AAGGTGCTGC CGGTCCCGCT GGTCCCTCCG GTCCCAGAGG TCCCCCTGGT 
CCCGTCGGCC CCTCTGGCAA AGATGGCTCT AACGGCATGC CCGGCCCCAT CGGTCCTCCC 
GGTCCCCGTG GACGGAGTGG TGAACCCGGC CCTGCGGGTC CTCCTGGAAA CCCCGGTCCT 
CCCGGTCCTC CTGGCCCCCC CGGCACCGGC ATCGACATGT CTGCTTTTGC TGGACTGGGT 
CAGACGGAGA AGGGCCCCGA CCCCATCCGC TACATGAGGG CAGACGAGGC GGCCGGAGGG 
CTGCGGCAGC ACGACGTGGA GGTGGATGCC ACCCTCAAAT CCCTCAACAA TCAGATTGAG 
AGCATCCGCA GCCCCGAGGG CTCCAAGAAG AACCCTGCCA GGACCTGCCG CGACATCAAA 
CTCTGCCATC CCGAGTGGAA GAGCGGAGAT TACTGGATTG ACCCGAACCA GGGCTGCACC 
TTGGACGCCA TCAAAGTATT CTGCAACATG GAGACGGGCG AGACCTGCGT CTACCCGACC 
CCCAGCAGCA TCCCCAGGAA GAACTGGTGG ACCAGCAAGA CGAAAGACAA GAAGCACGTC 
TGGTTTGCAG AGACCATCAA CGGCGGTTTC CACTTCAGCT ACGGCGATGA GAACCTGTCC 
CCCAACACCG CCAGCATCCA GATGACCTTC CTGCGCCTCC TGTCCACCGA GGGCTCCCAG 
AACGTCACCT ACCACTGCAA GAACAGCATC GCCTACATGG ACGAGGAGAC GGGCAACCTG 
AAGAAAGCCA TCCTCATCCA GGGATCCAAC GACGTGGAGA TCAGAGCCGA GGGCAACAGC 
AGGTTCACCT ACAGCGTCTT GGAGGACGGC TGCACGAAAC ACACTGGCAA ATGGGGCAAG 
ACGGTGATCG AGTACCGGTT GCAGAAGACC TCGCGCCTGT CCATTGTAGA TACTGCACCT 
ATGGACATTG GCGGAGCCGA TCAGGAGTTT GGCGTGGATA TTGGCCCAGT CTGCTTCTTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Interaction of lubricin with type II collagen surfaces: adsorption, friction, and normal forces.
Journal of biomechanics47(3)659-66(2014 Feb)
Chang DP,Guilak F,Jay GD,Zauscher S

Molecular cloning, characterization and localization of chicken type II procollagen gene.
Gene366(1)67-76(2006 Jan)
Xi C,Liu N,Liang F,Guo S,Sun Y,Yang F,Xi Y

Structure and sequence of the chicken type II procollagen gene. Characterization of the region encoding the carboxyl-terminal telopeptide and propeptide.
The Journal of biological chemistry259(12)7826-34(1984 Jun)
Sandell LJ,Prentice HL,Kravis D,Upholt WB

Isolation of cDNA and genomic DNA clones encoding type II collagen.
Nucleic acids research12(10)4207-28(1984 May)
Young MF,Vogeli G,Nunez AM,Fernandez MP,Sullivan M,Sobel ME

Identification of genomic DNA coding for chicken type II procollagen.
The Journal of biological chemistry258(19)11617-21(1983 Oct)
Sandell LJ,Yamada Y,Dorfman A,Upholt WB

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COL2A1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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