CHEK1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHEK1
Entrez Gene ID	374260
Full Name	checkpoint kinase 1
General protein information	Preferred Names checkpoint kinase 1 Names serine/threonine-protein kinase Chk1 CHK1 checkpoint homolog STT3, subunit of the oligosaccharyltransferase complex, homolog A integral membrane protein 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015297941.1	XP_015153427.1	serine/threonine-protein kinase Chk1 isoform X2
NM_204345.1	NP_989676.1	serine/threonine-protein kinase Chk1
XM_015297939.1	XP_015153425.1	serine/threonine-protein kinase Chk1 isoform X1
XM_015297939.2	XP_015153425.1	serine/threonine-protein kinase Chk1 isoform X1
XM_015297941.2	XP_015153427.1	serine/threonine-protein kinase Chk1 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CHEK1	NP_001091492.1
Gallus gallus (chicken)	STT3A	NP_989676.1
Saccharomyces cerevisiae (baker's yeast)	CHK1	NP_009833.1
Eremothecium gossypii	AGOS_AEL185C	NP_984676.1
Magnaporthe oryzae (rice blast fungus)	MGG_03729	XP_003716151.1
Xenopus tropicalis (tropical clawed frog)	chek1	XP_002937561.2
Homo sapiens (human)	CHEK1	NP_001265.2
Rattus norvegicus (Norway rat)	Chek1	NP_536325.1
Caenorhabditis elegans (roundworm)	chk-1	NP_001024233.1
Kluyveromyces lactis	KLLA0F01276g	XP_455137.1
Schizosaccharomyces pombe (fission yeast)	chk1	NP_588070.1
	LOC713358	XP_001111431.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008118	XP_317338.4
Pan troglodytes (chimpanzee)	CHEK1	XP_001146296.1
Mus musculus (house mouse)	Chek1	NP_031717.2
Neurospora crassa	NCU08346	XP_965249.2
Canis lupus familiaris (dog)	CHEK1	XP_852191.1
Danio rerio (zebrafish)	chek1	NP_956487.1
Drosophila melanogaster (fruit fly)	grp	NP_723986.1

Gene Ontology
Bibliography

Related articles in PubMed

Opposing effects of pericentrin and microcephalin on the pericentriolar material regulate CHK1 activation in the DNA damage response.
Antonczak AK, Mullee LI, Wang Y, Comartin D, Inoue T, Pelletier L, Morrison CG
Oncogene35(15)2003-10(2016 Apr)

BRCA1 Is Required for Maintenance of Phospho-Chk1 and G2/M Arrest during DNA Cross-Link Repair in DT40 Cells.
Draga M, Madgett EB, Vandenberg CJ, du Plessis D, Kaufmann A, Werler P, Chakraborty P, Lowndes NF, Hiom K
Molecular and cellular biology35(22)3829-40(2015 Nov)

DNA damage induces Chk1-dependent threonine-160 phosphorylation and activation of Cdk2.
Bourke E, Brown JA, Takeda S, Hochegger H, Morrison CG
Oncogene29(4)616-24(2010 Jan)

Chk1 C-terminal regulatory phosphorylation mediates checkpoint activation by de-repression of Chk1 catalytic activity.
Walker M, Black EJ, Oehler V, Gillespie DA, Scott MT
Oncogene28(24)2314-23(2009 Jun)

Expanded roles for Chk1 in genome maintenance.
Enders GH
The Journal of biological chemistry283(26)17749-52(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pericentriolar material expansion depended on CHK1 kinase. Nuclear CHK1 activation after DNA damage mirrored PCM expansion, suggesting a PCM/damage feedback loop whereby CHK1 regulates pericentrin-dependent PCM expansion to control its own activation.
Title: Opposing effects of pericentrin and microcephalin on the pericentriolar material regulate CHK1 activation in the DNA damage response.

BRCA1 promotes G2 arrest and cell death by prolonging phosphorylation of Chk1 on serine 345 after DNA damage to sustain arrest.
Title: BRCA1 Is Required for Maintenance of Phospho-Chk1 and G2/M Arrest during DNA Cross-Link Repair in DT40 Cells.

Chk1 signalling causes centrosome amplification after ionizing radiation by upregulating Cdk2 activity through activating phosphorylation.
Title: DNA damage induces Chk1-dependent threonine-160 phosphorylation and activation of Cdk2.

Results show that S345 phosphorylation is impaired in Chk1 S317A and S366A mutants, suggesting that modification of adjacent SQ sites promotes this key regulatory event.
Title: Chk1 C-terminal regulatory phosphorylation mediates checkpoint activation by de-repression of Chk1 catalytic activity.

Chk1 appears to help defend genomic integrity through effects on several other pathways, including Fanconi anemia proteins, the mitotic spindle, and transcription of cell cycle-related genes [review]
Title: Expanded roles for Chk1 in genome maintenance.

The following CHEK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHEK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa27688	NM_204345.1 Latest version!	Gallus gallus checkpoint kinase 1 (CHEK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OGa45778	XM_015297941.2 Latest version!	Gallus gallus checkpoint kinase 1 (CHEK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OGa45777	XM_015297939.2 Latest version!	Gallus gallus checkpoint kinase 1 (CHEK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OGa45777	XM_015297939.1	Gallus gallus checkpoint kinase 1 (CHEK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OGa45778	XM_015297941.1	Gallus gallus checkpoint kinase 1 (CHEK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHEK1 ( NM_204345.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_989676.1 Gallus gallus checkpoint kinase 1 (CHEK1), mRNA.

Price & Availability↑

CloneID	OGa27688
Clone ID Related Accession (Same CDS sequence)	NM_204345.1
Accession Version	NM_204345.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase Chk1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF525027.1. On Dec 9, 2006 this sequence version replaced XM_001231581.1. ##Evidence-Data-START## Transcript exon combination :: AF525027.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCGGTGC CCTTCGTGGA GGACTGGGAC CTGGTGCAGA CCCTGGGAGA AGGCGCTTAC 
GGGGAGGTGC AGTTGGCCGT GAACCGCCGC ACCGAGGAGG CCGTGGCCGT GAAGATCGTG 
GACATGAAGC GCGCGGCCGA CTGCCCGGAG AACATCAAGA AGGAGATCTG CATCAACAAG 
ATGCTGAACC ACGAGAACGT CGTCAAGTTC TACGGGCACC GGCGGGAGGG GGCCACGCAG 
TACCTGTTCC TGGAGTACTG CAGCGGCGGG GAGCTCTTCG ACCGCATCGA GCCGGACATT 
GGGATGCCGG AGCCGGAGGC GCAGCGCTTC TTCCAGCAGC TGATTGCTGG TGTGGTGTAC 
CTGCACAGCA TGGGCATCAC GCACCGCGAC CTCAAGCCGG AGAACCTGCT GCTGGATGAG 
CGAGACAACC TGAAGATCTC CGATTTCGGC CTGGCCACCG TCTTCAAGCA CAACGGGCGC 
GAGCGGCTGC TGAACAAGAT GTGTGGCACA CTGCCCTACG TGGCCCCCGA GCTGCTGCGG 
CGCCCCGAGT TCCGTGCGGA GCCCGTGGAC GTGTGGGCCT GCGGCGTGGT GCTGACCGCC 
ATGCTGGCGG GAGAGCTACC CTGGGATCAG CCCAGCGACA GCTGCCAGGA GTACAGCGAC 
TGGAAGGAGA GGAAGACCTA CCTGGCGCCC TGGAGGAAGA TCGACTCTGC ACCATTAGCA 
CTGCTGCACA AGATCCTGAC CGAGAACCCC ACGGCGCGCA TCACCATCCC CGACATCAAG 
AAGGACCGCT GGTACTGCCG GCCCCTGAAG AAGGGCACAA AGCGTGGCCG CGTGTCCTCA 
GGTGGTGTCA CCGAGTCCCC CGGAGCCCTC CCCAAACACA TCCGCTCTGA CACAGACTTC 
TCTCCTGTGA AGAGTGCCCT GGGTGAGGAC AAGGCGAGCT ACTCCACATC ACAGCCTGAG 
CCCGGCACCG GCGGCGCGCT GTGGGACAGC AGCACGGGCA GCATCGACCG CCTGGTGCAG 
GGCATCAGCT TCTCCCAGCC TGCCTGCCCT GAGCACATGC TGCTCAACAG CCAGCTGCTC 
GGCACGCCAG GCTCCTCCCA GAGCCCGTGG CAGCGCCTGG TGAGGCGGAT GACGCGCTTC 
TTCACCAAGC TGGACGCCGA CGGCTCCTAC CGCAGCCTGA GGGACGTGTG TGAGAAGATG 
GGCTACGGCT GGAAGCAGAG CTGCACCAAC CAGGTCACCA TCTCCACCAC GGACCGGAGG 
AACAACAAGC TGATCTTCAA GGTGAACCTG CTGGAGATGG AGAGCAGGAT CCTGGTGGAC 
TTCCGCCTCT CCAAGGGCGA CGGGTTGGAG TTCAAGCGGC ATTTCCTGAA AATCAAAGGC 
AAACTGAGCG ACGTGGTCAG CACCCAGAAG GTGTGGCTGC CCCCACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989676.1
CDS	28..1458
Translation	MAVPFVEDWDLVQTLGEGAYGEVQLAVNRRTEEAVAVKIVDMKRAADCPENIKKEICINKMLNHENVVKFYGHRREGATQYLFLEYCSGGELFDRIEPDIGMPEPEAQRFFQQLIAGVVYLHSMGITHRDLKPENLLLDERDNLKISDFGLATVFKHNGRERLLNKMCGTLPYVAPELLRRPEFRAEPVDVWACGVVLTAMLAGELPWDQPSDSCQEYSDWKERKTYLAPWRKIDSAPLALLHKILTENPTARITIPDIKKDRWYCRPLKKGTKRGRVSSGGVTESPGALPKHIRSDTDFSPVKSALGEDKASYSTSQPEPGTGGALWDSSTGSIDRLVQGISFSQPACPEHMLLNSQLLGTPGSSQSPWQRLVRRMTRFFTKLDADGSYRSLRDVCEKMGYGWKQSCTNQVTISTTDRRNNKLIFKVNLLEMESRILVDFRLSKGDGLEFKRHFLKIKGKLSDVVSTQKVWLPPP

Target ORF information:

RefSeq Version	NM_204345.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus checkpoint kinase 1 (CHEK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204345.1

ORF Insert Sequence:

ATGGCGGTGC CCTTCGTGGA GGACTGGGAC CTGGTGCAGA CCCTGGGAGA AGGCGCTTAC 
GGGGAGGTGC AGTTGGCCGT GAACCGCCGC ACCGAGGAGG CCGTGGCCGT GAAGATCGTG 
GACATGAAGC GCGCGGCCGA CTGCCCGGAG AACATCAAGA AGGAGATCTG CATCAACAAG 
ATGCTGAACC ACGAGAACGT CGTCAAGTTC TACGGGCACC GGCGGGAGGG GGCCACGCAG 
TACCTGTTCC TGGAGTACTG CAGCGGCGGG GAGCTCTTCG ACCGCATCGA GCCGGACATT 
GGGATGCCGG AGCCGGAGGC GCAGCGCTTC TTCCAGCAGC TGATTGCTGG TGTGGTGTAC 
CTGCACAGCA TGGGCATCAC GCACCGCGAC CTCAAGCCGG AGAACCTGCT GCTGGATGAG 
CGAGACAACC TGAAGATCTC CGATTTCGGC CTGGCCACCG TCTTCAAGCA CAACGGGCGC 
GAGCGGCTGC TGAACAAGAT GTGTGGCACA CTGCCCTACG TGGCCCCCGA GCTGCTGCGG 
CGCCCCGAGT TCCGTGCGGA GCCCGTGGAC GTGTGGGCCT GCGGCGTGGT GCTGACCGCC 
ATGCTGGCGG GAGAGCTACC CTGGGATCAG CCCAGCGACA GCTGCCAGGA GTACAGCGAC 
TGGAAGGAGA GGAAGACCTA CCTGGCGCCC TGGAGGAAGA TCGACTCTGC ACCATTAGCA 
CTGCTGCACA AGATCCTGAC CGAGAACCCC ACGGCGCGCA TCACCATCCC CGACATCAAG 
AAGGACCGCT GGTACTGCCG GCCCCTGAAG AAGGGCACAA AGCGTGGCCG CGTGTCCTCA 
GGTGGTGTCA CCGAGTCCCC CGGAGCCCTC CCCAAACACA TCCGCTCTGA CACAGACTTC 
TCTCCTGTGA AGAGTGCCCT GGGTGAGGAC AAGGCGAGCT ACTCCACATC ACAGCCTGAG 
CCCGGCACCG GCGGCGCGCT GTGGGACAGC AGCACGGGCA GCATCGACCG CCTGGTGCAG 
GGCATCAGCT TCTCCCAGCC TGCCTGCCCT GAGCACATGC TGCTCAACAG CCAGCTGCTC 
GGCACGCCAG GCTCCTCCCA GAGCCCGTGG CAGCGCCTGG TGAGGCGGAT GACGCGCTTC 
TTCACCAAGC TGGACGCCGA CGGCTCCTAC CGCAGCCTGA GGGACGTGTG TGAGAAGATG 
GGCTACGGCT GGAAGCAGAG CTGCACCAAC CAGGTCACCA TCTCCACCAC GGACCGGAGG 
AACAACAAGC TGATCTTCAA GGTGAACCTG CTGGAGATGG AGAGCAGGAT CCTGGTGGAC 
TTCCGCCTCT CCAAGGGCGA CGGGTTGGAG TTCAAGCGGC ATTTCCTGAA AATCAAAGGC 
AAACTGAGCG ACGTGGTCAG CACCCAGAAG GTGTGGCTGC CCCCACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHEK1 ( XM_015297941.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015153427.1 Gallus gallus checkpoint kinase 1 (CHEK1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa45778
Clone ID Related Accession (Same CDS sequence)	XM_015297941.1 , XM_015297941.2
Accession Version	XM_015297941.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase Chk1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456007.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGC CCTTCGTGGA GGACTGGGAC CTGGTGCAGA CCCTGGGAGA AGGCGCTTAC 
GGGGAGGTGC AGTTGGCCGT GAACCGCCGC ACCGAGGAGG CCGTGGCCGT GAAGATCGTG 
GACATGAAGC GCGCGGCCGA CTGCCCGGAG AACATCAAGA AGGAGATCTG CATCAACAAG 
ATGCTGAACC ACGAGAACGT CGTCAAGTTC TACGGGCACC GGCGGGAGGG GGCCACGCAG 
TACCTGTTCC TGGAGTACTG CAGCGGCGGG GAGCTCTTCG ACCGCATCGA GCCGGACATT 
GGGATGCCGG AGCCGGAGGC GCAGCGCTTC TTCCAGCAGC TGATTGCTGG TGTGGTGTAC 
CTGCACAGCA TGGGCATCAC GCACCGCGAC CTCAAGCCGG AGAACCTGCT GCTGGATGAG 
CGAGACAACC TGAAGATCTC CGATTTCGGC CTGGCCACCG TCTTCAAGCA CAACGGGCGC 
GAGCGGCTGC TGAACAAGAT GTGTGGCACA CTGCCCTACG TGGCCCCCGA GCTGCTGCGG 
CGCCCCGAGT TCCGTGCAGA GCCCGTGGAC GTGTGGGCCT GCGGCGTGGT GCTGACCGCC 
ATGCTGGCGG GAGAGCTACC CTGGGATCAG CCCAGCGACA GCTGCCAGGA GTACAGCGAC 
TGGAAGGAGA GGAAGACCTA CCTGGCGCCC TGGAGGAAGA TCGACTCTGC ACCATTAGCA 
CTGCTGCACA AGATCCTGAC CGAGAACCCC ACGGCGCGCA TCACCATCCC CGACATCAAG 
AAGGACCGCT GGTACTGCCG GCCCCTGAAG AAGGGTGGTG TCACCGAGTC CCCCGGAGCC 
CTCCCCAAAC ACATCCGCTC TGACACAGAC TTCTCTCCTG TGAAGAGTGC CCTGGGTGAG 
GACAAGGCGA GCTACTCCAC GTCGCAGCCT GAGCCCGGCA CCGGCGGTGC GCTGTGGGAC 
AGCAGCACGG GCAGCATCGA CCGCCTGGTG CAGGGCATCA GCTTCTCCCA GCCTGCCTGC 
CCTGAGCACA TGCTGCTCAA CAGCCAGCTG CTCGGCACAC CAGGCTCCTC CCAGAGCCCG 
TGGCAGCGCC TGGTGAGGCG GATGACGCGC TTCTTCACCA AGCTGGACGC CGACGGCTCC 
TACCGCAGCC TGAGGGACGT GTGTGAGAAG ATGGGCTACG GCTGGAAGCA GAGCTGCACC 
AACCAGGTCA CCATCTCCAC CACGGACCGG AGGAACAACA AGCTGATCTT CAAGGTGAAC 
CTGCTGGAGA TGGAGAGCAG GATCCTGGTG GACTTCCGCC TCTCCAAGGG CGACGGGTTG 
GAGTTCAAGC GGCATTTCCT GAAAATCAAA GGCAAACTGA GCGACGTGGT CAGCACCCAG 
AAGGTGTGGC TGCCCCCACC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015153427.1
CDS	76..1479
Translation	MAVPFVEDWDLVQTLGEGAYGEVQLAVNRRTEEAVAVKIVDMKRAADCPENIKKEICINKMLNHENVVKFYGHRREGATQYLFLEYCSGGELFDRIEPDIGMPEPEAQRFFQQLIAGVVYLHSMGITHRDLKPENLLLDERDNLKISDFGLATVFKHNGRERLLNKMCGTLPYVAPELLRRPEFRAEPVDVWACGVVLTAMLAGELPWDQPSDSCQEYSDWKERKTYLAPWRKIDSAPLALLHKILTENPTARITIPDIKKDRWYCRPLKKGGVTESPGALPKHIRSDTDFSPVKSALGEDKASYSTSQPEPGTGGALWDSSTGSIDRLVQGISFSQPACPEHMLLNSQLLGTPGSSQSPWQRLVRRMTRFFTKLDADGSYRSLRDVCEKMGYGWKQSCTNQVTISTTDRRNNKLIFKVNLLEMESRILVDFRLSKGDGLEFKRHFLKIKGKLSDVVSTQKVWLPPP

Target ORF information:

RefSeq Version	XM_015297941.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus checkpoint kinase 1 (CHEK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297941.1

ORF Insert Sequence:

ATGGCGGTGC CCTTCGTGGA GGACTGGGAC CTGGTGCAGA CCCTGGGAGA AGGCGCTTAC 
GGGGAGGTGC AGTTGGCCGT GAACCGCCGC ACCGAGGAGG CCGTGGCCGT GAAGATCGTG 
GACATGAAGC GCGCGGCCGA CTGCCCGGAG AACATCAAGA AGGAGATCTG CATCAACAAG 
ATGCTGAACC ACGAGAACGT CGTCAAGTTC TACGGGCACC GGCGGGAGGG GGCCACGCAG 
TACCTGTTCC TGGAGTACTG CAGCGGCGGG GAGCTCTTCG ACCGCATCGA GCCGGACATT 
GGGATGCCGG AGCCGGAGGC GCAGCGCTTC TTCCAGCAGC TGATTGCTGG TGTGGTGTAC 
CTGCACAGCA TGGGCATCAC GCACCGCGAC CTCAAGCCGG AGAACCTGCT GCTGGATGAG 
CGAGACAACC TGAAGATCTC CGATTTCGGC CTGGCCACCG TCTTCAAGCA CAACGGGCGC 
GAGCGGCTGC TGAACAAGAT GTGTGGCACA CTGCCCTACG TGGCCCCCGA GCTGCTGCGG 
CGCCCCGAGT TCCGTGCAGA GCCCGTGGAC GTGTGGGCCT GCGGCGTGGT GCTGACCGCC 
ATGCTGGCGG GAGAGCTACC CTGGGATCAG CCCAGCGACA GCTGCCAGGA GTACAGCGAC 
TGGAAGGAGA GGAAGACCTA CCTGGCGCCC TGGAGGAAGA TCGACTCTGC ACCATTAGCA 
CTGCTGCACA AGATCCTGAC CGAGAACCCC ACGGCGCGCA TCACCATCCC CGACATCAAG 
AAGGACCGCT GGTACTGCCG GCCCCTGAAG AAGGGTGGTG TCACCGAGTC CCCCGGAGCC 
CTCCCCAAAC ACATCCGCTC TGACACAGAC TTCTCTCCTG TGAAGAGTGC CCTGGGTGAG 
GACAAGGCGA GCTACTCCAC GTCGCAGCCT GAGCCCGGCA CCGGCGGTGC GCTGTGGGAC 
AGCAGCACGG GCAGCATCGA CCGCCTGGTG CAGGGCATCA GCTTCTCCCA GCCTGCCTGC 
CCTGAGCACA TGCTGCTCAA CAGCCAGCTG CTCGGCACAC CAGGCTCCTC CCAGAGCCCG 
TGGCAGCGCC TGGTGAGGCG GATGACGCGC TTCTTCACCA AGCTGGACGC CGACGGCTCC 
TACCGCAGCC TGAGGGACGT GTGTGAGAAG ATGGGCTACG GCTGGAAGCA GAGCTGCACC 
AACCAGGTCA CCATCTCCAC CACGGACCGG AGGAACAACA AGCTGATCTT CAAGGTGAAC 
CTGCTGGAGA TGGAGAGCAG GATCCTGGTG GACTTCCGCC TCTCCAAGGG CGACGGGTTG 
GAGTTCAAGC GGCATTTCCT GAAAATCAAA GGCAAACTGA GCGACGTGGT CAGCACCCAG 
AAGGTGTGGC TGCCCCCACC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHEK1 ( XM_015297941.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015153427.1 Gallus gallus checkpoint kinase 1 (CHEK1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa45778
Clone ID Related Accession (Same CDS sequence)	XM_015297941.1 , XM_015297941.2
Accession Version	XM_015297941.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase Chk1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006111.5) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015297941.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGC CCTTCGTGGA GGACTGGGAC CTGGTGCAGA CCCTGGGAGA AGGCGCTTAC 
GGGGAGGTGC AGTTGGCCGT GAACCGCCGC ACCGAGGAGG CCGTGGCCGT GAAGATCGTG 
GACATGAAGC GCGCGGCCGA CTGCCCGGAG AACATCAAGA AGGAGATCTG CATCAACAAG 
ATGCTGAACC ACGAGAACGT CGTCAAGTTC TACGGGCACC GGCGGGAGGG GGCCACGCAG 
TACCTGTTCC TGGAGTACTG CAGCGGCGGG GAGCTCTTCG ACCGCATCGA GCCGGACATT 
GGGATGCCGG AGCCGGAGGC GCAGCGCTTC TTCCAGCAGC TGATTGCTGG TGTGGTGTAC 
CTGCACAGCA TGGGCATCAC GCACCGCGAC CTCAAGCCGG AGAACCTGCT GCTGGATGAG 
CGAGACAACC TGAAGATCTC CGATTTCGGC CTGGCCACCG TCTTCAAGCA CAACGGGCGC 
GAGCGGCTGC TGAACAAGAT GTGTGGCACA CTGCCCTACG TGGCCCCCGA GCTGCTGCGG 
CGCCCCGAGT TCCGTGCAGA GCCCGTGGAC GTGTGGGCCT GCGGCGTGGT GCTGACCGCC 
ATGCTGGCGG GAGAGCTACC CTGGGATCAG CCCAGCGACA GCTGCCAGGA GTACAGCGAC 
TGGAAGGAGA GGAAGACCTA CCTGGCGCCC TGGAGGAAGA TCGACTCTGC ACCATTAGCA 
CTGCTGCACA AGATCCTGAC CGAGAACCCC ACGGCGCGCA TCACCATCCC CGACATCAAG 
AAGGACCGCT GGTACTGCCG GCCCCTGAAG AAGGGTGGTG TCACCGAGTC CCCCGGAGCC 
CTCCCCAAAC ACATCCGCTC TGACACAGAC TTCTCTCCTG TGAAGAGTGC CCTGGGTGAG 
GACAAGGCGA GCTACTCCAC GTCGCAGCCT GAGCCCGGCA CCGGCGGTGC GCTGTGGGAC 
AGCAGCACGG GCAGCATCGA CCGCCTGGTG CAGGGCATCA GCTTCTCCCA GCCTGCCTGC 
CCTGAGCACA TGCTGCTCAA CAGCCAGCTG CTCGGCACAC CAGGCTCCTC CCAGAGCCCG 
TGGCAGCGCC TGGTGAGGCG GATGACGCGC TTCTTCACCA AGCTGGACGC CGACGGCTCC 
TACCGCAGCC TGAGGGACGT GTGTGAGAAG ATGGGCTACG GCTGGAAGCA GAGCTGCACC 
AACCAGGTCA CCATCTCCAC CACGGACCGG AGGAACAACA AGCTGATCTT CAAGGTGAAC 
CTGCTGGAGA TGGAGAGCAG GATCCTGGTG GACTTCCGCC TCTCCAAGGG CGACGGGTTG 
GAGTTCAAGC GGCATTTCCT GAAAATCAAA GGCAAACTGA GCGACGTGGT CAGCACCCAG 
AAGGTGTGGC TGCCCCCACC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015153427.1
CDS	82..1485
Translation	MAVPFVEDWDLVQTLGEGAYGEVQLAVNRRTEEAVAVKIVDMKRAADCPENIKKEICINKMLNHENVVKFYGHRREGATQYLFLEYCSGGELFDRIEPDIGMPEPEAQRFFQQLIAGVVYLHSMGITHRDLKPENLLLDERDNLKISDFGLATVFKHNGRERLLNKMCGTLPYVAPELLRRPEFRAEPVDVWACGVVLTAMLAGELPWDQPSDSCQEYSDWKERKTYLAPWRKIDSAPLALLHKILTENPTARITIPDIKKDRWYCRPLKKGGVTESPGALPKHIRSDTDFSPVKSALGEDKASYSTSQPEPGTGGALWDSSTGSIDRLVQGISFSQPACPEHMLLNSQLLGTPGSSQSPWQRLVRRMTRFFTKLDADGSYRSLRDVCEKMGYGWKQSCTNQVTISTTDRRNNKLIFKVNLLEMESRILVDFRLSKGDGLEFKRHFLKIKGKLSDVVSTQKVWLPPP

Target ORF information:

RefSeq Version	XM_015297941.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus checkpoint kinase 1 (CHEK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297941.2

ORF Insert Sequence:

ATGGCGGTGC CCTTCGTGGA GGACTGGGAC CTGGTGCAGA CCCTGGGAGA AGGCGCTTAC 
GGGGAGGTGC AGTTGGCCGT GAACCGCCGC ACCGAGGAGG CCGTGGCCGT GAAGATCGTG 
GACATGAAGC GCGCGGCCGA CTGCCCGGAG AACATCAAGA AGGAGATCTG CATCAACAAG 
ATGCTGAACC ACGAGAACGT CGTCAAGTTC TACGGGCACC GGCGGGAGGG GGCCACGCAG 
TACCTGTTCC TGGAGTACTG CAGCGGCGGG GAGCTCTTCG ACCGCATCGA GCCGGACATT 
GGGATGCCGG AGCCGGAGGC GCAGCGCTTC TTCCAGCAGC TGATTGCTGG TGTGGTGTAC 
CTGCACAGCA TGGGCATCAC GCACCGCGAC CTCAAGCCGG AGAACCTGCT GCTGGATGAG 
CGAGACAACC TGAAGATCTC CGATTTCGGC CTGGCCACCG TCTTCAAGCA CAACGGGCGC 
GAGCGGCTGC TGAACAAGAT GTGTGGCACA CTGCCCTACG TGGCCCCCGA GCTGCTGCGG 
CGCCCCGAGT TCCGTGCAGA GCCCGTGGAC GTGTGGGCCT GCGGCGTGGT GCTGACCGCC 
ATGCTGGCGG GAGAGCTACC CTGGGATCAG CCCAGCGACA GCTGCCAGGA GTACAGCGAC 
TGGAAGGAGA GGAAGACCTA CCTGGCGCCC TGGAGGAAGA TCGACTCTGC ACCATTAGCA 
CTGCTGCACA AGATCCTGAC CGAGAACCCC ACGGCGCGCA TCACCATCCC CGACATCAAG 
AAGGACCGCT GGTACTGCCG GCCCCTGAAG AAGGGTGGTG TCACCGAGTC CCCCGGAGCC 
CTCCCCAAAC ACATCCGCTC TGACACAGAC TTCTCTCCTG TGAAGAGTGC CCTGGGTGAG 
GACAAGGCGA GCTACTCCAC GTCGCAGCCT GAGCCCGGCA CCGGCGGTGC GCTGTGGGAC 
AGCAGCACGG GCAGCATCGA CCGCCTGGTG CAGGGCATCA GCTTCTCCCA GCCTGCCTGC 
CCTGAGCACA TGCTGCTCAA CAGCCAGCTG CTCGGCACAC CAGGCTCCTC CCAGAGCCCG 
TGGCAGCGCC TGGTGAGGCG GATGACGCGC TTCTTCACCA AGCTGGACGC CGACGGCTCC 
TACCGCAGCC TGAGGGACGT GTGTGAGAAG ATGGGCTACG GCTGGAAGCA GAGCTGCACC 
AACCAGGTCA CCATCTCCAC CACGGACCGG AGGAACAACA AGCTGATCTT CAAGGTGAAC 
CTGCTGGAGA TGGAGAGCAG GATCCTGGTG GACTTCCGCC TCTCCAAGGG CGACGGGTTG 
GAGTTCAAGC GGCATTTCCT GAAAATCAAA GGCAAACTGA GCGACGTGGT CAGCACCCAG 
AAGGTGTGGC TGCCCCCACC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHEK1 ( XM_015297939.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015153425.1 Gallus gallus checkpoint kinase 1 (CHEK1), transcript variant X1, mRNA.

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CloneID	OGa45777
Clone ID Related Accession (Same CDS sequence)	XM_015297939.1 , XM_015297939.2
Accession Version	XM_015297939.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase Chk1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456007.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGC CCTTCGTGGA GGACTGGGAC CTGGTGCAGA CCCTGGGAGA AGGCGCTTAC 
GGGGAGGTGC AGTTGGCCGT GAACCGCCGC ACCGAGGAGG CCGTGGCCGT GAAGATCGTG 
GACATGAAGC GCGCGGCCGA CTGCCCGGAG AACATCAAGA AGGAGATCTG CATCAACAAG 
ATGCTGAACC ACGAGAACGT CGTCAAGTTC TACGGGCACC GGCGGGAGGG GGCCACGCAG 
TACCTGTTCC TGGAGTACTG CAGCGGCGGG GAGCTCTTCG ACCGCATCGA GCCGGACATT 
GGGATGCCGG AGCCGGAGGC GCAGCGCTTC TTCCAGCAGC TGATTGCTGG TGTGGTGTAC 
CTGCACAGCA TGGGCATCAC GCACCGCGAC CTCAAGCCGG AGAACCTGCT GCTGGATGAG 
CGAGACAACC TGAAGATCTC CGATTTCGGC CTGGCCACCG TCTTCAAGCA CAACGGGCGC 
GAGCGGCTGC TGAACAAGAT GTGTGGCACA CTGCCCTACG TGGCCCCCGA GCTGCTGCGG 
CGCCCCGAGT TCCGTGCAGA GCCCGTGGAC GTGTGGGCCT GCGGCGTGGT GCTGACCGCC 
ATGCTGGCGG GAGAGCTACC CTGGGATCAG CCCAGCGACA GCTGCCAGGA GTACAGCGAC 
TGGAAGGAGA GGAAGACCTA CCTGGCGCCC TGGAGGAAGA TCGACTCTGC ACCATTAGCA 
CTGCTGCACA AGATCCTGAC CGAGAACCCC ACGGCGCGCA TCACCATCCC CGACATCAAG 
AAGGACCGCT GGTACTGCCG GCCCCTGAAG AAGGGTAAGA GCTGCGGGGC AAAGTGCGTT 
TCCTGCTCGA GCTGTGCACA GTGTTGGGTT TTTGCTCGCT TTGCAGGCAC AAAGCGTGGC 
CGCGTGTCCT CAGGTGGTGT CACCGAGTCC CCCGGAGCCC TCCCCAAACA CATCCGCTCT 
GACACAGACT TCTCTCCTGT GAAGAGTGCC CTGGGTGAGG ACAAGGCGAG CTACTCCACG 
TCGCAGCCTG AGCCCGGCAC CGGCGGTGCG CTGTGGGACA GCAGCACGGG CAGCATCGAC 
CGCCTGGTGC AGGGCATCAG CTTCTCCCAG CCTGCCTGCC CTGAGCACAT GCTGCTCAAC 
AGCCAGCTGC TCGGCACACC AGGCTCCTCC CAGAGCCCGT GGCAGCGCCT GGTGAGGCGG 
ATGACGCGCT TCTTCACCAA GCTGGACGCC GACGGCTCCT ACCGCAGCCT GAGGGACGTG 
TGTGAGAAGA TGGGCTACGG CTGGAAGCAG AGCTGCACCA ACCAGGTCAC CATCTCCACC 
ACGGACCGGA GGAACAACAA GCTGATCTTC AAGGTGAACC TGCTGGAGAT GGAGAGCAGG 
ATCCTGGTGG ACTTCCGCCT CTCCAAGGGC GACGGGTTGG AGTTCAAGCG GCATTTCCTG 
AAAATCAAAG GCAAACTGAG CGACGTGGTC AGCACCCAGA AGGTGTGGCT GCCCCCACCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015153425.1
CDS	76..1578
Translation	MAVPFVEDWDLVQTLGEGAYGEVQLAVNRRTEEAVAVKIVDMKRAADCPENIKKEICINKMLNHENVVKFYGHRREGATQYLFLEYCSGGELFDRIEPDIGMPEPEAQRFFQQLIAGVVYLHSMGITHRDLKPENLLLDERDNLKISDFGLATVFKHNGRERLLNKMCGTLPYVAPELLRRPEFRAEPVDVWACGVVLTAMLAGELPWDQPSDSCQEYSDWKERKTYLAPWRKIDSAPLALLHKILTENPTARITIPDIKKDRWYCRPLKKGKSCGAKCVSCSSCAQCWVFARFAGTKRGRVSSGGVTESPGALPKHIRSDTDFSPVKSALGEDKASYSTSQPEPGTGGALWDSSTGSIDRLVQGISFSQPACPEHMLLNSQLLGTPGSSQSPWQRLVRRMTRFFTKLDADGSYRSLRDVCEKMGYGWKQSCTNQVTISTTDRRNNKLIFKVNLLEMESRILVDFRLSKGDGLEFKRHFLKIKGKLSDVVSTQKVWLPPP

Target ORF information:

RefSeq Version	XM_015297939.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus checkpoint kinase 1 (CHEK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297939.1

ORF Insert Sequence:

ATGGCGGTGC CCTTCGTGGA GGACTGGGAC CTGGTGCAGA CCCTGGGAGA AGGCGCTTAC 
GGGGAGGTGC AGTTGGCCGT GAACCGCCGC ACCGAGGAGG CCGTGGCCGT GAAGATCGTG 
GACATGAAGC GCGCGGCCGA CTGCCCGGAG AACATCAAGA AGGAGATCTG CATCAACAAG 
ATGCTGAACC ACGAGAACGT CGTCAAGTTC TACGGGCACC GGCGGGAGGG GGCCACGCAG 
TACCTGTTCC TGGAGTACTG CAGCGGCGGG GAGCTCTTCG ACCGCATCGA GCCGGACATT 
GGGATGCCGG AGCCGGAGGC GCAGCGCTTC TTCCAGCAGC TGATTGCTGG TGTGGTGTAC 
CTGCACAGCA TGGGCATCAC GCACCGCGAC CTCAAGCCGG AGAACCTGCT GCTGGATGAG 
CGAGACAACC TGAAGATCTC CGATTTCGGC CTGGCCACCG TCTTCAAGCA CAACGGGCGC 
GAGCGGCTGC TGAACAAGAT GTGTGGCACA CTGCCCTACG TGGCCCCCGA GCTGCTGCGG 
CGCCCCGAGT TCCGTGCAGA GCCCGTGGAC GTGTGGGCCT GCGGCGTGGT GCTGACCGCC 
ATGCTGGCGG GAGAGCTACC CTGGGATCAG CCCAGCGACA GCTGCCAGGA GTACAGCGAC 
TGGAAGGAGA GGAAGACCTA CCTGGCGCCC TGGAGGAAGA TCGACTCTGC ACCATTAGCA 
CTGCTGCACA AGATCCTGAC CGAGAACCCC ACGGCGCGCA TCACCATCCC CGACATCAAG 
AAGGACCGCT GGTACTGCCG GCCCCTGAAG AAGGGTAAGA GCTGCGGGGC AAAGTGCGTT 
TCCTGCTCGA GCTGTGCACA GTGTTGGGTT TTTGCTCGCT TTGCAGGCAC AAAGCGTGGC 
CGCGTGTCCT CAGGTGGTGT CACCGAGTCC CCCGGAGCCC TCCCCAAACA CATCCGCTCT 
GACACAGACT TCTCTCCTGT GAAGAGTGCC CTGGGTGAGG ACAAGGCGAG CTACTCCACG 
TCGCAGCCTG AGCCCGGCAC CGGCGGTGCG CTGTGGGACA GCAGCACGGG CAGCATCGAC 
CGCCTGGTGC AGGGCATCAG CTTCTCCCAG CCTGCCTGCC CTGAGCACAT GCTGCTCAAC 
AGCCAGCTGC TCGGCACACC AGGCTCCTCC CAGAGCCCGT GGCAGCGCCT GGTGAGGCGG 
ATGACGCGCT TCTTCACCAA GCTGGACGCC GACGGCTCCT ACCGCAGCCT GAGGGACGTG 
TGTGAGAAGA TGGGCTACGG CTGGAAGCAG AGCTGCACCA ACCAGGTCAC CATCTCCACC 
ACGGACCGGA GGAACAACAA GCTGATCTTC AAGGTGAACC TGCTGGAGAT GGAGAGCAGG 
ATCCTGGTGG ACTTCCGCCT CTCCAAGGGC GACGGGTTGG AGTTCAAGCG GCATTTCCTG 
AAAATCAAAG GCAAACTGAG CGACGTGGTC AGCACCCAGA AGGTGTGGCT GCCCCCACCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHEK1 ( XM_015297939.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015153425.1 Gallus gallus checkpoint kinase 1 (CHEK1), transcript variant X1, mRNA.

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CloneID	OGa45777
Clone ID Related Accession (Same CDS sequence)	XM_015297939.1 , XM_015297939.2
Accession Version	XM_015297939.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase Chk1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006111.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015297939.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGC CCTTCGTGGA GGACTGGGAC CTGGTGCAGA CCCTGGGAGA AGGCGCTTAC 
GGGGAGGTGC AGTTGGCCGT GAACCGCCGC ACCGAGGAGG CCGTGGCCGT GAAGATCGTG 
GACATGAAGC GCGCGGCCGA CTGCCCGGAG AACATCAAGA AGGAGATCTG CATCAACAAG 
ATGCTGAACC ACGAGAACGT CGTCAAGTTC TACGGGCACC GGCGGGAGGG GGCCACGCAG 
TACCTGTTCC TGGAGTACTG CAGCGGCGGG GAGCTCTTCG ACCGCATCGA GCCGGACATT 
GGGATGCCGG AGCCGGAGGC GCAGCGCTTC TTCCAGCAGC TGATTGCTGG TGTGGTGTAC 
CTGCACAGCA TGGGCATCAC GCACCGCGAC CTCAAGCCGG AGAACCTGCT GCTGGATGAG 
CGAGACAACC TGAAGATCTC CGATTTCGGC CTGGCCACCG TCTTCAAGCA CAACGGGCGC 
GAGCGGCTGC TGAACAAGAT GTGTGGCACA CTGCCCTACG TGGCCCCCGA GCTGCTGCGG 
CGCCCCGAGT TCCGTGCAGA GCCCGTGGAC GTGTGGGCCT GCGGCGTGGT GCTGACCGCC 
ATGCTGGCGG GAGAGCTACC CTGGGATCAG CCCAGCGACA GCTGCCAGGA GTACAGCGAC 
TGGAAGGAGA GGAAGACCTA CCTGGCGCCC TGGAGGAAGA TCGACTCTGC ACCATTAGCA 
CTGCTGCACA AGATCCTGAC CGAGAACCCC ACGGCGCGCA TCACCATCCC CGACATCAAG 
AAGGACCGCT GGTACTGCCG GCCCCTGAAG AAGGGTAAGA GCTGCGGGGC AAAGTGCGTT 
TCCTGCTCGA GCTGTGCACA GTGTTGGGTT TTTGCTCGCT TTGCAGGCAC AAAGCGTGGC 
CGCGTGTCCT CAGGTGGTGT CACCGAGTCC CCCGGAGCCC TCCCCAAACA CATCCGCTCT 
GACACAGACT TCTCTCCTGT GAAGAGTGCC CTGGGTGAGG ACAAGGCGAG CTACTCCACG 
TCGCAGCCTG AGCCCGGCAC CGGCGGTGCG CTGTGGGACA GCAGCACGGG CAGCATCGAC 
CGCCTGGTGC AGGGCATCAG CTTCTCCCAG CCTGCCTGCC CTGAGCACAT GCTGCTCAAC 
AGCCAGCTGC TCGGCACACC AGGCTCCTCC CAGAGCCCGT GGCAGCGCCT GGTGAGGCGG 
ATGACGCGCT TCTTCACCAA GCTGGACGCC GACGGCTCCT ACCGCAGCCT GAGGGACGTG 
TGTGAGAAGA TGGGCTACGG CTGGAAGCAG AGCTGCACCA ACCAGGTCAC CATCTCCACC 
ACGGACCGGA GGAACAACAA GCTGATCTTC AAGGTGAACC TGCTGGAGAT GGAGAGCAGG 
ATCCTGGTGG ACTTCCGCCT CTCCAAGGGC GACGGGTTGG AGTTCAAGCG GCATTTCCTG 
AAAATCAAAG GCAAACTGAG CGACGTGGTC AGCACCCAGA AGGTGTGGCT GCCCCCACCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015153425.1
CDS	82..1584
Translation	MAVPFVEDWDLVQTLGEGAYGEVQLAVNRRTEEAVAVKIVDMKRAADCPENIKKEICINKMLNHENVVKFYGHRREGATQYLFLEYCSGGELFDRIEPDIGMPEPEAQRFFQQLIAGVVYLHSMGITHRDLKPENLLLDERDNLKISDFGLATVFKHNGRERLLNKMCGTLPYVAPELLRRPEFRAEPVDVWACGVVLTAMLAGELPWDQPSDSCQEYSDWKERKTYLAPWRKIDSAPLALLHKILTENPTARITIPDIKKDRWYCRPLKKGKSCGAKCVSCSSCAQCWVFARFAGTKRGRVSSGGVTESPGALPKHIRSDTDFSPVKSALGEDKASYSTSQPEPGTGGALWDSSTGSIDRLVQGISFSQPACPEHMLLNSQLLGTPGSSQSPWQRLVRRMTRFFTKLDADGSYRSLRDVCEKMGYGWKQSCTNQVTISTTDRRNNKLIFKVNLLEMESRILVDFRLSKGDGLEFKRHFLKIKGKLSDVVSTQKVWLPPP

Target ORF information:

RefSeq Version	XM_015297939.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus checkpoint kinase 1 (CHEK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297939.2

ORF Insert Sequence:

ATGGCGGTGC CCTTCGTGGA GGACTGGGAC CTGGTGCAGA CCCTGGGAGA AGGCGCTTAC 
GGGGAGGTGC AGTTGGCCGT GAACCGCCGC ACCGAGGAGG CCGTGGCCGT GAAGATCGTG 
GACATGAAGC GCGCGGCCGA CTGCCCGGAG AACATCAAGA AGGAGATCTG CATCAACAAG 
ATGCTGAACC ACGAGAACGT CGTCAAGTTC TACGGGCACC GGCGGGAGGG GGCCACGCAG 
TACCTGTTCC TGGAGTACTG CAGCGGCGGG GAGCTCTTCG ACCGCATCGA GCCGGACATT 
GGGATGCCGG AGCCGGAGGC GCAGCGCTTC TTCCAGCAGC TGATTGCTGG TGTGGTGTAC 
CTGCACAGCA TGGGCATCAC GCACCGCGAC CTCAAGCCGG AGAACCTGCT GCTGGATGAG 
CGAGACAACC TGAAGATCTC CGATTTCGGC CTGGCCACCG TCTTCAAGCA CAACGGGCGC 
GAGCGGCTGC TGAACAAGAT GTGTGGCACA CTGCCCTACG TGGCCCCCGA GCTGCTGCGG 
CGCCCCGAGT TCCGTGCAGA GCCCGTGGAC GTGTGGGCCT GCGGCGTGGT GCTGACCGCC 
ATGCTGGCGG GAGAGCTACC CTGGGATCAG CCCAGCGACA GCTGCCAGGA GTACAGCGAC 
TGGAAGGAGA GGAAGACCTA CCTGGCGCCC TGGAGGAAGA TCGACTCTGC ACCATTAGCA 
CTGCTGCACA AGATCCTGAC CGAGAACCCC ACGGCGCGCA TCACCATCCC CGACATCAAG 
AAGGACCGCT GGTACTGCCG GCCCCTGAAG AAGGGTAAGA GCTGCGGGGC AAAGTGCGTT 
TCCTGCTCGA GCTGTGCACA GTGTTGGGTT TTTGCTCGCT TTGCAGGCAC AAAGCGTGGC 
CGCGTGTCCT CAGGTGGTGT CACCGAGTCC CCCGGAGCCC TCCCCAAACA CATCCGCTCT 
GACACAGACT TCTCTCCTGT GAAGAGTGCC CTGGGTGAGG ACAAGGCGAG CTACTCCACG 
TCGCAGCCTG AGCCCGGCAC CGGCGGTGCG CTGTGGGACA GCAGCACGGG CAGCATCGAC 
CGCCTGGTGC AGGGCATCAG CTTCTCCCAG CCTGCCTGCC CTGAGCACAT GCTGCTCAAC 
AGCCAGCTGC TCGGCACACC AGGCTCCTCC CAGAGCCCGT GGCAGCGCCT GGTGAGGCGG 
ATGACGCGCT TCTTCACCAA GCTGGACGCC GACGGCTCCT ACCGCAGCCT GAGGGACGTG 
TGTGAGAAGA TGGGCTACGG CTGGAAGCAG AGCTGCACCA ACCAGGTCAC CATCTCCACC 
ACGGACCGGA GGAACAACAA GCTGATCTTC AAGGTGAACC TGCTGGAGAT GGAGAGCAGG 
ATCCTGGTGG ACTTCCGCCT CTCCAAGGGC GACGGGTTGG AGTTCAAGCG GCATTTCCTG 
AAAATCAAAG GCAAACTGAG CGACGTGGTC AGCACCCAGA AGGTGTGGCT GCCCCCACCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Opposing effects of pericentrin and microcephalin on the pericentriolar material regulate CHK1 activation in the DNA damage response.
Oncogene35(15)2003-10(2016 Apr)
Antonczak AK,Mullee LI,Wang Y,Comartin D,Inoue T,Pelletier L,Morrison CG

BRCA1 Is Required for Maintenance of Phospho-Chk1 and G2/M Arrest during DNA Cross-Link Repair in DT40 Cells.
Molecular and cellular biology35(22)3829-40(2015 Nov)
Draga M,Madgett EB,Vandenberg CJ,du Plessis D,Kaufmann A,Werler P,Chakraborty P,Lowndes NF,Hiom K

DNA damage induces Chk1-dependent threonine-160 phosphorylation and activation of Cdk2.
Oncogene29(4)616-24(2010 Jan)
Bourke E,Brown JA,Takeda S,Hochegger H,Morrison CG

Chk1 C-terminal regulatory phosphorylation mediates checkpoint activation by de-repression of Chk1 catalytic activity.
Oncogene28(24)2314-23(2009 Jun)
Walker M,Black EJ,Oehler V,Gillespie DA,Scott MT

Expanded roles for Chk1 in genome maintenance.
The Journal of biological chemistry283(26)17749-52(2008 Jun)
Enders GH

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CHEK1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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