CDKN1B cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	CDKN1B
Entrez Gene ID	374106
Full Name	cyclin dependent kinase inhibitor 1B
General protein information	Preferred Names cyclin dependent kinase inhibitor 1B Names cyclin-dependent kinase inhibitor 1B cyclin-dependent kinase inhibitor 1B (p27, Kip1)
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_204256.2	NP_989587.1	cyclin-dependent kinase inhibitor 1B
XM_015289915.1	XP_015145401.1	cyclin-dependent kinase inhibitor 1B isoform X1
XM_015289915.2	XP_015145401.1	cyclin-dependent kinase inhibitor 1B isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CDKN1B	XP_522347.3
Canis lupus familiaris (dog)	CDKN1B	NP_001002957.1
Bos taurus (cattle)	CDKN1B	NP_001093816.1
	CDKN1B	XP_001085433.1
Danio rerio (zebrafish)	cdkn1bb	NP_997957.1
Danio rerio (zebrafish)	LOC100534692	XP_003201420.1
Rattus norvegicus (Norway rat)	Cdkn1b	NP_113950.2
Homo sapiens (human)	CDKN1B	NP_004055.1
Mus musculus (house mouse)	Cdkn1b	NP_034005.2
Gallus gallus (chicken)	CDKN1B	NP_989587.1

Gene Ontology
Bibliography

Related articles in PubMed

p27(Kip1) participates in the regulation of endoreplication in differentiating chick retinal ganglion cells.
Ovejero-Benito MC, Frade JM
Cell cycle (Georgetown, Tex.)14(14)2311-22(2015)

MicroRNAs 221 and 222 target p27Kip1 in Marek's disease virus-transformed tumour cell line MSB-1.
Lambeth LS, Yao Y, Smith LP, Zhao Y, Nair V
The Journal of general virology90(Pt 5)1164-71(2009 May)

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Caldwell RB, Kierzek AM, Arakawa H, Bezzubov Y, Zaim J, Fiedler P, Kutter S, Blagodatski A, Kostovska D, Koter M, Plachy J, Carninci P, Hayashizaki Y, Buerstedde JM
Genome biology6(1)R6(2005)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The short hairpin RNAs (shRNA) that significantly downregulate p27(Kip1) expression facilitated DNA synthesis and increased ploidy in isolated chick RGCs.
Title: p27(Kip1) participates in the regulation of endoreplication in differentiating chick retinal ganglion cells.

These data show that an oncogenic herpesvirus, as in the case of many cancers, can exploit the miRNA machinery for suppressing cell cycle regulatory molecules such as p27(Kip1) in the induction and progression of T-cell lymphomas.
Title: MicroRNAs 221 and 222 target p27Kip1 in Marek's disease virus-transformed tumour cell line MSB-1.

The following CDKN1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDKN1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa25171	NM_204256.2 Latest version!	Gallus gallus cyclin dependent kinase inhibitor 1B (CDKN1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OGa29651	XM_015289915.2 Latest version!	Gallus gallus cyclin dependent kinase inhibitor 1B (CDKN1B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
View Old Accession Versions >>
OGa29651	XM_015289915.1	Gallus gallus cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CDKN1B ( NM_204256.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_989587.1 Gallus gallus cyclin dependent kinase inhibitor 1B (CDKN1B), mRNA.

Price & Availability↑

CloneID	OGa25171
Clone ID Related Accession (Same CDS sequence)	NM_204256.2
Accession Version	NM_204256.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-16
Organism	Gallus gallus(chicken)
Product	cyclin-dependent kinase inhibitor 1B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ851674.1. On Feb 27, 2005 this sequence version replaced NM_204256.1. ##Evidence-Data-START## Transcript exon combination :: AJ851674.1, BU407623.1 [ECO:0000332] ##Evidence-Data-END##

ATGTCAAACG TCCGCATTTC TAATGGGAGC CCTACCCTGG AGCGCATGGA GGCGCGGCAG 
TCGGAGTACC CGAAGCCGTC GGCCTGCAGG AACCTCTTCG GGCCGGTAAA CCACGAAGAG 
TTAAACAGGG ACTTGAAGAA GCACCGCAAG GAAATGGAAG AGGCATGCCA GAGGAAGTGG 
AATTTCGATT TCCAGAACCA CAAGCCGCTG GAAGGCAGGT ACGAGTGGCA AGCCGTGGAG 
AAGGGGAGCT CGCCCGACTT CTACTTCAGG CAGCCGAGGC TATCCAAAGC CGTCTGCAAG 
TCCGCCGGCC GTCAGAGCCT GGATGTAAAC GGGAATTGCC AAACCGCGAT TTGCGCCGCT 
TCTCAGGGAA TCTCAGAGGA CACTCACTGT GTAGGCCGAA AGACTGATGT TGCTGGCAGT 
CAGACGGACT TTGCAGAGCA GTGCGCCGGG CAGAGGAAAA GACCCGCCGC CGACGATTCC 
TCTCCTCAAA ATAAAAGAGC CAACACAACA GAAGAGGAGG TTTCAGAAGA CTCCCCCAGT 
GCCAGTTCAG TGGAGCAAAC ACCCAAGAAA TCGAGCCCGA GACGACATCA AACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989587.1
CDS	530..1126
Translation	MSNVRISNGSPTLERMEARQSEYPKPSACRNLFGPVNHEELNRDLKKHRKEMEEACQRKWNFDFQNHKPLEGRYEWQAVEKGSSPDFYFRQPRLSKAVCKSAGRQSLDVNGNCQTAICAASQGISEDTHCVGRKTDVAGSQTDFAEQCAGQRKRPAADDSSPQNKRANTTEEEVSEDSPSASSVEQTPKKSSPRRHQT

Target ORF information:

RefSeq Version	NM_204256.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus cyclin dependent kinase inhibitor 1B (CDKN1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204256.2

ORF Insert Sequence:

ATGTCAAACG TCCGCATTTC TAATGGGAGC CCTACCCTGG AGCGCATGGA GGCGCGGCAG 
TCGGAGTACC CGAAGCCGTC GGCCTGCAGG AACCTCTTCG GGCCGGTAAA CCACGAAGAG 
TTAAACAGGG ACTTGAAGAA GCACCGCAAG GAAATGGAAG AGGCATGCCA GAGGAAGTGG 
AATTTCGATT TCCAGAACCA CAAGCCGCTG GAAGGCAGGT ACGAGTGGCA AGCCGTGGAG 
AAGGGGAGCT CGCCCGACTT CTACTTCAGG CAGCCGAGGC TATCCAAAGC CGTCTGCAAG 
TCCGCCGGCC GTCAGAGCCT GGATGTAAAC GGGAATTGCC AAACCGCGAT TTGCGCCGCT 
TCTCAGGGAA TCTCAGAGGA CACTCACTGT GTAGGCCGAA AGACTGATGT TGCTGGCAGT 
CAGACGGACT TTGCAGAGCA GTGCGCCGGG CAGAGGAAAA GACCCGCCGC CGACGATTCC 
TCTCCTCAAA ATAAAAGAGC CAACACAACA GAAGAGGAGG TTTCAGAAGA CTCCCCCAGT 
GCCAGTTCAG TGGAGCAAAC ACCCAAGAAA TCGAGCCCGA GACGACATCA AACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDKN1B ( XM_015289915.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145401.1 Gallus gallus cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa29651
Clone ID Related Accession (Same CDS sequence)	XM_015289915.1 , XM_015289915.2
Accession Version	XM_015289915.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	cyclin-dependent kinase inhibitor 1B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455715.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAACG TCCGCATTTC TAATGGGAGC CCTACCCTGG AGCGCATGGA GGCGCGGCAG 
TCGGAGTACC CGAAGCCGTC GGCCTGCAGG AACCTCTTCG GGCCGGTAAA CCACGAAGAG 
TTAAACAGGG ACTTGAAGAA GCACCGCAAG GAAATGGAAG AGGCATGCCA GAGGAAGTGG 
AATTTCGATT TCCAGAACCA CAAGCCGCTG GAAGGCAGGT ACGAGTGGCA AGCCGTGGAG 
AAGGGGAGCT CGCCCGACTT CTACTTCAGG CAGCCGAGGC TATCCAAAGC CGTCTGCAAG 
TCCGCCGGCC GTCAGAGCCT GGATGTAAAC GGGAATTGCC AAACCGCGAT TTGCGCCGCT 
TCTCAGGGAA TCTCAGAGGA CACTCACTGT GTAGGCCGAA AGACTGATGT TGCTGGCAGT 
CAGACGGACT TTGCAGAGCA GTGCGCCGGG CAGAGGAAAA GACCCGCCGC CGACGGGCGG 
AGGACTCGCG TTTCCTTGCT CATCGGGGGG CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145401.1
CDS	523..1038
Translation	MSNVRISNGSPTLERMEARQSEYPKPSACRNLFGPVNHEELNRDLKKHRKEMEEACQRKWNFDFQNHKPLEGRYEWQAVEKGSSPDFYFRQPRLSKAVCKSAGRQSLDVNGNCQTAICAASQGISEDTHCVGRKTDVAGSQTDFAEQCAGQRKRPAADGRRTRVSLLIGGR

Target ORF information:

RefSeq Version	XM_015289915.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289915.1

ORF Insert Sequence:

ATGTCAAACG TCCGCATTTC TAATGGGAGC CCTACCCTGG AGCGCATGGA GGCGCGGCAG 
TCGGAGTACC CGAAGCCGTC GGCCTGCAGG AACCTCTTCG GGCCGGTAAA CCACGAAGAG 
TTAAACAGGG ACTTGAAGAA GCACCGCAAG GAAATGGAAG AGGCATGCCA GAGGAAGTGG 
AATTTCGATT TCCAGAACCA CAAGCCGCTG GAAGGCAGGT ACGAGTGGCA AGCCGTGGAG 
AAGGGGAGCT CGCCCGACTT CTACTTCAGG CAGCCGAGGC TATCCAAAGC CGTCTGCAAG 
TCCGCCGGCC GTCAGAGCCT GGATGTAAAC GGGAATTGCC AAACCGCGAT TTGCGCCGCT 
TCTCAGGGAA TCTCAGAGGA CACTCACTGT GTAGGCCGAA AGACTGATGT TGCTGGCAGT 
CAGACGGACT TTGCAGAGCA GTGCGCCGGG CAGAGGAAAA GACCCGCCGC CGACGGGCGG 
AGGACTCGCG TTTCCTTGCT CATCGGGGGG CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDKN1B ( XM_015289915.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145401.1 Gallus gallus cyclin dependent kinase inhibitor 1B (CDKN1B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa29651
Clone ID Related Accession (Same CDS sequence)	XM_015289915.1 , XM_015289915.2
Accession Version	XM_015289915.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	cyclin-dependent kinase inhibitor 1B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289915.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAACG TCCGCATTTC TAATGGGAGC CCTACCCTGG AGCGCATGGA GGCGCGGCAG 
TCGGAGTACC CGAAGCCGTC GGCCTGCAGG AACCTCTTCG GGCCGGTAAA CCACGAAGAG 
TTAAACAGGG ACTTGAAGAA GCACCGCAAG GAAATGGAAG AGGCATGCCA GAGGAAGTGG 
AATTTCGATT TCCAGAACCA CAAGCCGCTG GAAGGCAGGT ACGAGTGGCA AGCCGTGGAG 
AAGGGGAGCT CGCCCGACTT CTACTTCAGG CAGCCGAGGC TATCCAAAGC CGTCTGCAAG 
TCCGCCGGCC GTCAGAGCCT GGATGTAAAC GGGAATTGCC AAACCGCGAT TTGCGCCGCT 
TCTCAGGGAA TCTCAGAGGA CACTCACTGT GTAGGCCGAA AGACTGATGT TGCTGGCAGT 
CAGACGGACT TTGCAGAGCA GTGCGCCGGG CAGAGGAAAA GACCCGCCGC CGACGGGCGG 
AGGACTCGCG TTTCCTTGCT CATCGGGGGG CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145401.1
CDS	5817..6332
Translation	MSNVRISNGSPTLERMEARQSEYPKPSACRNLFGPVNHEELNRDLKKHRKEMEEACQRKWNFDFQNHKPLEGRYEWQAVEKGSSPDFYFRQPRLSKAVCKSAGRQSLDVNGNCQTAICAASQGISEDTHCVGRKTDVAGSQTDFAEQCAGQRKRPAADGRRTRVSLLIGGR

Target ORF information:

RefSeq Version	XM_015289915.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus cyclin dependent kinase inhibitor 1B (CDKN1B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289915.2

ORF Insert Sequence:

ATGTCAAACG TCCGCATTTC TAATGGGAGC CCTACCCTGG AGCGCATGGA GGCGCGGCAG 
TCGGAGTACC CGAAGCCGTC GGCCTGCAGG AACCTCTTCG GGCCGGTAAA CCACGAAGAG 
TTAAACAGGG ACTTGAAGAA GCACCGCAAG GAAATGGAAG AGGCATGCCA GAGGAAGTGG 
AATTTCGATT TCCAGAACCA CAAGCCGCTG GAAGGCAGGT ACGAGTGGCA AGCCGTGGAG 
AAGGGGAGCT CGCCCGACTT CTACTTCAGG CAGCCGAGGC TATCCAAAGC CGTCTGCAAG 
TCCGCCGGCC GTCAGAGCCT GGATGTAAAC GGGAATTGCC AAACCGCGAT TTGCGCCGCT 
TCTCAGGGAA TCTCAGAGGA CACTCACTGT GTAGGCCGAA AGACTGATGT TGCTGGCAGT 
CAGACGGACT TTGCAGAGCA GTGCGCCGGG CAGAGGAAAA GACCCGCCGC CGACGGGCGG 
AGGACTCGCG TTTCCTTGCT CATCGGGGGG CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

p27(Kip1) participates in the regulation of endoreplication in differentiating chick retinal ganglion cells.
Cell cycle (Georgetown, Tex.)14(14)2311-22(2015)
Ovejero-Benito MC,Frade JM

MicroRNAs 221 and 222 target p27Kip1 in Marek's disease virus-transformed tumour cell line MSB-1.
The Journal of general virology90(Pt 5)1164-71(2009 May)
Lambeth LS,Yao Y,Smith LP,Zhao Y,Nair V

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Genome biology6(1)R6(2005)
Caldwell RB,Kierzek AM,Arakawa H,Bezzubov Y,Zaim J,Fiedler P,Kutter S,Blagodatski A,Kostovska D,Koter M,Plachy J,Carninci P,Hayashizaki Y,Buerstedde JM

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CDKN1B cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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