MYH1B cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYH1B
Entrez Gene ID	374069
Full Name	myosin, heavy chain 1B, skeletal muscle
Synonyms	MYH3
General protein information	Preferred Names myosin, heavy chain 1B, skeletal muscle Names myosin, heavy chain 1B, skeletal muscle myosin heavy chain 3 myosin heavy chain, fast skeletal muscle, embryonic myosin, heavy chain 1B, skeletal muscle (similar to human myosin, heavy chain 1, skeletal muscle, adult) myosin-1B myosin-3
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_204228.3	NP_989559.2	myosin, heavy chain 1B, skeletal muscle

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	MYH1C	NP_001107181.1
Mus musculus (house mouse)	Myh2	NP_001034634.2
Gallus gallus (chicken)	MYH1D	XP_415578.4
Gallus gallus (chicken)	MYH1E	NP_001013415.1
Bos taurus (cattle)	MYH2	NP_001159699.1
	MYH2	XP_002800336.1
Canis lupus familiaris (dog)	MYH2	NP_001070263.1
Gallus gallus (chicken)	MYH1A	NP_001013414.1
Gallus gallus (chicken)	MYH1F	XP_001231409.1
Homo sapiens (human)	MYH2	NP_001093582.1
Rattus norvegicus (Norway rat)	Myh2	NP_001128629.1
Gallus gallus (chicken)	MYH1B	NP_989559.1
Gallus gallus (chicken)	MYH1G	XP_003642358.1

Gene Ontology
Bibliography

Related articles in PubMed

EMX2 activates slow myosin heavy chain 2 gene expression in embryonic muscle fibers.
Hatch K, Pabon A, DiMario JX
Mechanisms of development1478-16(2017 Oct)

Muscle fiber type specific induction of slow myosin heavy chain 2 gene expression by electrical stimulation.
Crew JR, Falzari K, DiMario JX
Experimental cell research316(6)1039-49(2010 Apr)

Betaine protects urea-induced denaturation of myosin subfragment-1.
Ortiz-Costa S, Sorenson MM, Sola-Penna M
The FEBS journal275(13)3388-96(2008 Jul)

Conserved Asp-137 imparts flexibility to tropomyosin and affects function.
Sumida JP, Wu E, Lehrer SS
The Journal of biological chemistry283(11)6728-34(2008 Mar)

Evidence for the expression of neonatal skeletal myosin heavy chain in primary myocardium and cardiac conduction tissue in the developing chick heart.
Machida S, Matsuoka R, Noda S, Hiratsuka E, Takagaki Y, Oana S, Furutani Y, Nakajima H, Takao A, Momma K
Developmental dynamics : an official publication of the American Association of Anatomists217(1)37-49(2000 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results indicate that slow muscle fibers can be induced by electrical stimulation to express the slow MyHC2 gene and that fast muscle fibers are refractory to stimulation-induced slow MyHC2 gene expression.
Title: Muscle fiber type specific induction of slow myosin heavy chain 2 gene expression by electrical stimulation.

urea-induced inactivation of the enzyme is caused by partial unfolding of the myosin catalytic domain
Title: Betaine protects urea-induced denaturation of myosin subfragment-1.

the highly conserved Asp-137 destabilizes the middle of Tm, resulting in a more flexible region that is important for the cooperative activation of the thin filament by myosin
Title: Conserved Asp-137 imparts flexibility to tropomyosin and affects function.

The following MYH1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYH1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa28304	NM_204228.3 Latest version!	Gallus gallus myosin, heavy chain 1B, skeletal muscle (similar to human myosin, heavy chain 1, skeletal muscle, adult) (MYH1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYH1B ( NM_204228.3 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_989559.2 Gallus gallus myosin, heavy chain 1B, skeletal muscle (similar to human myosin, heavy chain 1, skeletal muscle, adult) (MYH1B), mRNA.

Price & Availability↑

CloneID	OGa28304
Clone ID Related Accession (Same CDS sequence)	NM_204228.3
Accession Version	NM_204228.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-11
Organism	Gallus gallus(chicken)
Product	myosin, heavy chain 1B, skeletal muscle
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000628.1. On Feb 9, 2016 this sequence version replaced NM_204228.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF272033.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTCTC CAGATGCTGA AATGGCCGCC TTTGGGGAGG CTGCTCCTTA TCTTCGAAAG 
TCAGAGAAGG AACGAATCGA GGCCCAGAAC AAGCCTTTTG ATGCCAAGTC ATCAGTCTTT 
GTGGTGCATC CCAAAGAATC TTTTGTGAAA GGGACTATCC AGAGCAAAGA AACAGGGAAG 
GTCACTGTCA AGACTGAAGG TGGAGAAACT CTAACTGTGA AGGAAGATCA AATCTTCTCC 
ATGAATCCTC CCAAGTACGA TAAAATTGAG GACATGGCCA TGATGACCCA CCTCCACGAA 
CCCGCTGTGC TGTACAACCT CAAAGAGCGT TATGCAGCCT GGATGATCTA CACCTACTCG 
GGCCTCTTCT GTGTCACTGT CAACCCCTAC AAGTGGCTGC CGGTGTACAA CCCGGAGGTG 
GTGTTGGCCT ACCGAGGCAA AAAGCGCCAG GAGGCCCCTC CACACATTTT CTCCATTTCT 
GACAACGCCT ATCAGTTCAT GCTGACTGAC CGTGAGAATC AGTCGATCCT GATCACCGGA 
GAATCTGGTG CAGGGAAGAC TGTGAACACG AAACGTGTCA TCCAGTACTT TGCAACAATT 
GCAGCTAGTG GGGAGAAGAA GAAGGAGGAG CAGCCTGGCA AAATGCAGGG AACGCTTGAG 
GATCAAATCA TCAGCGCCAA CCCATTGCTA GAGGCCTTTG GAAATGCCAA GACTGTGAGG 
AATGACAACT CCTCACGCTT TGGGAAATTC ATCAGAATTC ACTTTGGTGC CACAGGAAAA 
CTAGCTTCTG CAGATATTGA AACATATCTA TTGGAGAAGT CTAGAGTCAC TTTTCAGCTG 
AAGGCAGAAA GAAGCTACCA CATATTTTAT CAAATTATGT CCAACAAGAA ACCAGAGCTA 
ATTGACATGC TTCTAATTAC CACCAATCCA TACGACTATC ACTTTGTGAG TCAAGGTGAG 
ATCACTGTTC CCAGCATTAA TGATCAAGAG GAGCTGATGG CTACAGATAG TGCCATTGAC 
ATCCTGGGCT TTACTGCCGA TGAAAAAGTG GCTATTTACA AGCTGACAGG AGCTGTCATG 
CACTATGGCA ACCTGAAGTT CAAGCAGAAA CAAAGAGAAG AGCAAGCAGA GCCAGATGGC 
ACAGAAGTTG CAGACAAGGC TGCCTACCTG ATGGGTCTGA ACTCAGCAGA CCTGCTCAAG 
GCCCTGTGCT ATCCTCGTGT CAAGGTTGGG AATGAGTACG TGACCAAGGG TCAAACTGTG 
CAGCAGGTGA ACAACTCAGT TGGTGCTCTG GCAAAGGCTG TCTATGAGAA GATGTTCTTG 
TGGATGGTTG TTCGCATCAA CCAACAGTTG GATACCAAGC AGCCCAGGCA GTACTTCATT 
GGTGTCCTGG ACATCGCTGG ATTTGAGATC TTTGATTTCA ACAGCTTGGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTCTTCAACC ACCACATGTT CGTGCTGGAA 
CAGGAGGAGT ACAAGAAGGA AGGCATTGAA TGGGAGTTCA TTGACTTTGG GATGGACCTG 
GCTGCTTGCA TTGAGCTCAT TGAAAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCAAC TGACACCTCT TTCAAAAACA AGCTCTATGA CCAGCATCTG 
GGCAAGTCCA GCAACTTCCA GAAGCCCAAG CCTGCCAAAG GCAAGGCTGA GGCCCACTTC 
TCTCTGGTGC ACTATGCTGG CACAGTAGAC TACAACATCA CTGGCTGGCT GGAGAAGAAC 
AAGGACCCCC TGAACGAAAC TGTCATTGGG CTATACCAGA AATCATCTGT GAAGACACTG 
GCCTTACTGT TTGCCAACTA TGGTGGAGCA GAAGCAGAGG CTAGTGGTGG TGGTGGTGGT 
GGCAAGAAAG GTGGCAAGAA GAAGGGTTCT TCTTTCCAGA CTGTCTCAGC CCTTTTCCGG 
GAGAACCTAA ACAAACTGAT GACCAACCTA CGGAGTACTC ACCCTCATTT TGTTCGCTGC 
ATCATCCCAA ATGAAACAAA AACACCTGGT GCCATGGAGC ATGAACTGGT GCTGCACCAG 
CTGCGCTGTA ACGGCGTGCT GGAGGGGATC AGGATTTGCA GGAAAGGCTT CCCTAGCAGA 
GTCCTCTATG CAGACTTTAA ACAGAGGTAC AAGGTGCTTA ATGCCAGTGC CATCCCTGAG 
GGACAGTTCA TTGATAGCAA GAAAGCTTCT GAGAAGCTTC TTGGGTCAAT TGATGTGGAC 
CACACCCAGT ACAAATTTGG TCACACCAAG GTGTTCTTCA AAGCTGGGCT GCTGGGACTC 
CTGGAGGAGA TGAGAGATGA GAAGCTGGCA CAGCTCATCA CTCGCACACA GGCCAGGTGC 
AGGGGCTTCC TGATGAGAGT GGAGTACCAG AGAATGGTGG AGAGGAGGGA ATCCATCTTC 
TGCATTCAGT ACAATGTTCG TGCATTCATG AATGTCAAGC ACTGGCCCTG GATGAAGCTG 
TTCTTCAAGA TCAAGCCCCT GCTGAAGAGC GCAGAGTCTG AGAAGGAGAT GGCCAACATG 
AAGGAAGAAT TTGAGAAGAC CAAGGAGGAG CTTGCAAAGT CTGAGGCAAA GAGGAAGGAG 
CTGGAGGAGA AAATGGTGAA GCTGGTGCAG GAGAAAAATG ATCTGCAGCT CCAAGTGCAG 
GCTGAAGCTG ATGCTTTGGC TGATGCTGAG GAAAGGTGTG ACCAGCTCAT CAAAACCAAA 
ATCCAGCTGG AAGCCAAAAT TAAGGAGGTG ACAGAACGGG CTGAGGATGA GGAGGAAATT 
AATGCTGAAC TGACAGCCAA GAAGAGGAAA CTGGAGGATG AATGCTCAGA GCTGAAGAAA 
GATATAGATG ACCTGGAGTT AACATTGGCC AAAGTTGAGA AGGAAAAGCA TGCCACTGAA 
AACAAGGTGA AAAACCTCAC AGAAGAGATG GCAGCCCTGG ATGAGACAAT TGCCAAGCTG 
ACAAAAGAGA AGAAAGCCCT CCAAGAGGCC CATCAGCAGA CACTGGATGA CCTGCAGGCT 
GAAGAGGACA AAGTCAATAC GCTGACCAAA GCTAAAACCA AGCTGGAACA GCAAGTGGAC 
GATCTGGAAG GGTCCCTGGA GCAAGAGAAG AAGCTGCGCA TGGACCTCGA GAGAGCTAAG 
AGGAAACTTG AAGGAGACCT GAAGATGTCC CAGGATACCA TAATGGATTT GGAAAATGAT 
AAACAGCAGC TGGATGAGAA ACTGAAGAAG AAAGACTTTG AAATCAGCCA GATCCAGAGC 
AAAATTGAGG ATGAGCAAGC CCTGGGCATG CAGTTACAGA AGAAGATCAA GGAGCTGCAG 
GCCCGTATTG AGGAACTGGA GGAGGAAATC GAGGCAGAGC GAACCTCTCG GGCAAAAGCA 
GAGAAGCACC GAGCTGACCT CTCCAGGGAG CTGGAGGAGA TCAGTGAGCG CCTGGAGGAA 
GCAGGAGGGG CTACAGCAGC TCAGATTGAT ATGAACAAGA AGCGTGAGGC AGAATTTCAG 
AAGATGCGCC GTGACCTCGA AGAGGCCACG CTGCAGCACG AAGCCACGGC TGCTGCCCTG 
CGGAAGAAGC ATGCGGACAG CACAGCGGAG CTTGGGGAGC AGATCGACAA CCTGCAACGT 
GTCAAGCAGA AGCTGGAGAA GGAGAAGAGT GAGCTGAAGA TGGAGATTGA TGACTTGGCC 
AGCAACATGG AGTCTGTCTC CAAAGCTAAG GCAAACCTGG AAAAGATGTG TCGTACTTTA 
GAAGACCAAC TGAGTGAGAT TAAGTCAAAG GAGGAGGAGC ATCAACGCAT GATAAACGAT 
CTCAGCACTC AAAGAGCTCG TCTGCAGACA GAATCAGGTG AATATTCACG CCAGGTGGAG 
GAGAAAGATG CCCTGATATC TCAGCTGTCT CGAGGCAAGC AGGCATTCAC CCAACAGATT 
GAGGAACTCA AGCGGCACTT AGAGGAAGAA ATCAAGGCCA AGAACGCCCT GGCCCACGCC 
TTGCAGTCTG CTCGTCATGA CTGTGATTTG CTCCGGGAAC AATATGAAGA GGAGCAGGAA 
GCCAAGGGTG AGCTGCAGCG TGCCCTGTCC AAGGCCAACA GTGAAGTGGC CCAGTGGAGA 
ACCAAATATG AGACAGATGC TATTCAGCGC ACAGAGGAAC TGGAGGAGGC CAAGAAGAAG 
CTGGCACAGC GCCTGCAGGA TGCCGAGGAA CATGTTGAAG CTGTGAATGC CAAATGTGCT 
TCCCTGGAAA AGACAAAGCA GAGGCTGCAG AATGAGGTGG AGGACCTGAT GATTGACGTG 
GAGCGAGCAA ATGCTGCCTG CGCAGCTCTG GACAAGAAGC AGAAGAACTT TGACAAGATC 
CTGGCAGAGT GGAAGCAGAA GTACGAGGAA ACGCAGGCTG AGCTGGAGGC CTCCCAGAAG 
GAGTCTCGCT CCCTCAGCAC AGAGCTGTTT AAGATGAAGA ATGCCTATGA GGAATCCCTG 
GACCACCTGG AAACGCTGAA GCGTGAGAAC AAGAACTTGC AGCAGGAGAT TTCTGACCTC 
ACGGAGCAGA TTGCAGAGGG AGGAAAGGCA ATTCATGAGT TGGAGAAAGT CAAGAAGCAG 
ATTGAGCAGG AGAAGTCTGA AATCCAGGCT GCTCTGGAAG AAGCTGAGGC CTCCCTGGAA 
CATGAAGAGG GGAAGATCCT GCGCCTCCAA CTGGAGCTCA ACCAGGTCAA GTCTGAGATT 
GACAGGAAAA TAGCAGAGAA AGATGAGGAA ATTGACCAGC TGAAGAGAAA TCACCTCAGA 
ATTGTGGAGT CCATGCAGAG CACTCTGGAT GCTGAGATCA GGAGCAGGAA TGAAGCCCTT 
AGACTGAAGA AGAAGATGGA GGGAGACCTG AATGAAATGG AGATCCAGCT CAACCATGCC 
AACCGCGTGG CTGCAGAGGC ACAAAAGAAC CTTAGAAACA CACAAGGAGT GCTCAAAGAT 
ACCCAGATAC ACTTGGATGA TGCTCTCAGG ACACAGGAGG ACCTGAAGGA GCAGGTGGCC 
ATGGTGGAGC GCAGAGCAAA CCTGTTGCAG GCTGAGATTG AGGAGCTACG GGCAGCCCTG 
GAACAGACAG AGCGCTCGAG AAAAGTGGCT GAGCAGGAAT TGATGGATGC AAGTGAGAGA 
GTTCAGCTCC TCCACACTCA GAACACCAGC TTGATCAACA CCAAGAAGAA ACTGGAAACA 
GACATTGCCC AAATTCAGAG TGAAATGGAG GATACCATCC AGGAAGCCCG CAATGCTGAA 
GAGAAGGCCA AGAAGGCCAT CACCGATGCA GCCATGATGG CAGAAGAGCT GAAGAAGGAG 
CAGGACACCA GTGCCCACCT GGAGAGGATG AAGAAGAACC TGGACCAGAC GGTGAAGGAT 
CTGCAGCTCC GTCTGGATGA GGCAGAGCAG CTGGCCCTGA AGGGAGGCAA GAAGCAAATT 
CAGAAGCTGG AGGCCAGGGT GCGGGAGCTG GAAGGGGAGG TTGATGCTGA GCAGAAGCGC 
AGCGCTGAAG CCGTGAAGGG TGTGCGCAAG TACGAGAGGA GGGTGAAGGA GCTGACATAC 
CAGTCTGAGG AAGATCGCAA GAACATTCTT AGACTCCAGG ATCTGGTGGA CAAACTGCAG 
ATGAAGGTGA AATCCTACAA GAGACAAGCT GAGGAGGCTG AGGAGCTGTC CAATGTCAAC 
CTCACCAAGT TCCGCAAGAT CCAGCATGAG CTGGAGGAAG CCGAGGAGCG GGCTGACATT 
GCAGAGTCAC AGGTCAACAA GCTCCGAGCA AAGAGCCGGG AGTTTCATGG CAAGAAGATA 
GAAGAGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989559.2
CDS	106..5940
Translation	MASPDAEMAAFGEAAPYLRKSEKERIEAQNKPFDAKSSVFVVHPKESFVKGTIQSKETGKVTVKTEGGETLTVKEDQIFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVLAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAASGEKKKEEQPGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPELIDMLLITTNPYDYHFVSQGEITVPSINDQEELMATDSAIDILGFTADEKVAIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLMGLNSADLLKALCYPRVKVGNEYVTKGQTVQQVNNSVGALAKAVYEKMFLWMVVRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSSNFQKPKPAKGKAEAHFSLVHYAGTVDYNITGWLEKNKDPLNETVIGLYQKSSVKTLALLFANYGGAEAEASGGGGGGKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRVLYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIDVDHTQYKFGHTKVFFKAGLLGLLEEMRDEKLAQLITRTQARCRGFLMRVEYQRMVERRESIFCIQYNVRAFMNVKHWPWMKLFFKIKPLLKSAESEKEMANMKEEFEKTKEELAKSEAKRKELEEKMVKLVQEKNDLQLQVQAEADALADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAALDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKMSQDTIMDLENDKQQLDEKLKKKDFEISQIQSKIEDEQALGMQLQKKIKELQARIEELEEEIEAERTSRAKAEKHRADLSRELEEISERLEEAGGATAAQIDMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSTAELGEQIDNLQRVKQKLEKEKSELKMEIDDLASNMESVSKAKANLEKMCRTLEDQLSEIKSKEEEHQRMINDLSTQRARLQTESGEYSRQVEEKDALISQLSRGKQAFTQQIEELKRHLEEEIKAKNALAHALQSARHDCDLLREQYEEEQEAKGELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERANAACAALDKKQKNFDKILAEWKQKYEETQAELEASQKESRSLSTELFKMKNAYEESLDHLETLKRENKNLQQEISDLTEQIAEGGKAIHELEKVKKQIEQEKSEIQAALEEAEASLEHEEGKILRLQLELNQVKSEIDRKIAEKDEEIDQLKRNHLRIVESMQSTLDAEIRSRNEALRLKKKMEGDLNEMEIQLNHANRVAAEAQKNLRNTQGVLKDTQIHLDDALRTQEDLKEQVAMVERRANLLQAEIEELRAALEQTERSRKVAEQELMDASERVQLLHTQNTSLINTKKKLETDIAQIQSEMEDTIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLDQTVKDLQLRLDEAEQLALKGGKKQIQKLEARVRELEGEVDAEQKRSAEAVKGVRKYERRVKELTYQSEEDRKNILRLQDLVDKLQMKVKSYKRQAEEAEELSNVNLTKFRKIQHELEEAEERADIAESQVNKLRAKSREFHGKKIEEEE

Target ORF information:

RefSeq Version	NM_204228.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus myosin, heavy chain 1B, skeletal muscle (similar to human myosin, heavy chain 1, skeletal muscle, adult) (MYH1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204228.3

ORF Insert Sequence:

ATGGCTTCTC CAGATGCTGA AATGGCCGCC TTTGGGGAGG CTGCTCCTTA TCTTCGAAAG 
TCAGAGAAGG AACGAATCGA GGCCCAGAAC AAGCCTTTTG ATGCCAAGTC ATCAGTCTTT 
GTGGTGCATC CCAAAGAATC TTTTGTGAAA GGGACTATCC AGAGCAAAGA AACAGGGAAG 
GTCACTGTCA AGACTGAAGG TGGAGAAACT CTAACTGTGA AGGAAGATCA AATCTTCTCC 
ATGAATCCTC CCAAGTACGA TAAAATTGAG GACATGGCCA TGATGACCCA CCTCCACGAA 
CCCGCTGTGC TGTACAACCT CAAAGAGCGT TATGCAGCCT GGATGATCTA CACCTACTCG 
GGCCTCTTCT GTGTCACTGT CAACCCCTAC AAGTGGCTGC CGGTGTACAA CCCGGAGGTG 
GTGTTGGCCT ACCGAGGCAA AAAGCGCCAG GAGGCCCCTC CACACATTTT CTCCATTTCT 
GACAACGCCT ATCAGTTCAT GCTGACTGAC CGTGAGAATC AGTCGATCCT GATCACCGGA 
GAATCTGGTG CAGGGAAGAC TGTGAACACG AAACGTGTCA TCCAGTACTT TGCAACAATT 
GCAGCTAGTG GGGAGAAGAA GAAGGAGGAG CAGCCTGGCA AAATGCAGGG AACGCTTGAG 
GATCAAATCA TCAGCGCCAA CCCATTGCTA GAGGCCTTTG GAAATGCCAA GACTGTGAGG 
AATGACAACT CCTCACGCTT TGGGAAATTC ATCAGAATTC ACTTTGGTGC CACAGGAAAA 
CTAGCTTCTG CAGATATTGA AACATATCTA TTGGAGAAGT CTAGAGTCAC TTTTCAGCTG 
AAGGCAGAAA GAAGCTACCA CATATTTTAT CAAATTATGT CCAACAAGAA ACCAGAGCTA 
ATTGACATGC TTCTAATTAC CACCAATCCA TACGACTATC ACTTTGTGAG TCAAGGTGAG 
ATCACTGTTC CCAGCATTAA TGATCAAGAG GAGCTGATGG CTACAGATAG TGCCATTGAC 
ATCCTGGGCT TTACTGCCGA TGAAAAAGTG GCTATTTACA AGCTGACAGG AGCTGTCATG 
CACTATGGCA ACCTGAAGTT CAAGCAGAAA CAAAGAGAAG AGCAAGCAGA GCCAGATGGC 
ACAGAAGTTG CAGACAAGGC TGCCTACCTG ATGGGTCTGA ACTCAGCAGA CCTGCTCAAG 
GCCCTGTGCT ATCCTCGTGT CAAGGTTGGG AATGAGTACG TGACCAAGGG TCAAACTGTG 
CAGCAGGTGA ACAACTCAGT TGGTGCTCTG GCAAAGGCTG TCTATGAGAA GATGTTCTTG 
TGGATGGTTG TTCGCATCAA CCAACAGTTG GATACCAAGC AGCCCAGGCA GTACTTCATT 
GGTGTCCTGG ACATCGCTGG ATTTGAGATC TTTGATTTCA ACAGCTTGGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTCTTCAACC ACCACATGTT CGTGCTGGAA 
CAGGAGGAGT ACAAGAAGGA AGGCATTGAA TGGGAGTTCA TTGACTTTGG GATGGACCTG 
GCTGCTTGCA TTGAGCTCAT TGAAAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCAAC TGACACCTCT TTCAAAAACA AGCTCTATGA CCAGCATCTG 
GGCAAGTCCA GCAACTTCCA GAAGCCCAAG CCTGCCAAAG GCAAGGCTGA GGCCCACTTC 
TCTCTGGTGC ACTATGCTGG CACAGTAGAC TACAACATCA CTGGCTGGCT GGAGAAGAAC 
AAGGACCCCC TGAACGAAAC TGTCATTGGG CTATACCAGA AATCATCTGT GAAGACACTG 
GCCTTACTGT TTGCCAACTA TGGTGGAGCA GAAGCAGAGG CTAGTGGTGG TGGTGGTGGT 
GGCAAGAAAG GTGGCAAGAA GAAGGGTTCT TCTTTCCAGA CTGTCTCAGC CCTTTTCCGG 
GAGAACCTAA ACAAACTGAT GACCAACCTA CGGAGTACTC ACCCTCATTT TGTTCGCTGC 
ATCATCCCAA ATGAAACAAA AACACCTGGT GCCATGGAGC ATGAACTGGT GCTGCACCAG 
CTGCGCTGTA ACGGCGTGCT GGAGGGGATC AGGATTTGCA GGAAAGGCTT CCCTAGCAGA 
GTCCTCTATG CAGACTTTAA ACAGAGGTAC AAGGTGCTTA ATGCCAGTGC CATCCCTGAG 
GGACAGTTCA TTGATAGCAA GAAAGCTTCT GAGAAGCTTC TTGGGTCAAT TGATGTGGAC 
CACACCCAGT ACAAATTTGG TCACACCAAG GTGTTCTTCA AAGCTGGGCT GCTGGGACTC 
CTGGAGGAGA TGAGAGATGA GAAGCTGGCA CAGCTCATCA CTCGCACACA GGCCAGGTGC 
AGGGGCTTCC TGATGAGAGT GGAGTACCAG AGAATGGTGG AGAGGAGGGA ATCCATCTTC 
TGCATTCAGT ACAATGTTCG TGCATTCATG AATGTCAAGC ACTGGCCCTG GATGAAGCTG 
TTCTTCAAGA TCAAGCCCCT GCTGAAGAGC GCAGAGTCTG AGAAGGAGAT GGCCAACATG 
AAGGAAGAAT TTGAGAAGAC CAAGGAGGAG CTTGCAAAGT CTGAGGCAAA GAGGAAGGAG 
CTGGAGGAGA AAATGGTGAA GCTGGTGCAG GAGAAAAATG ATCTGCAGCT CCAAGTGCAG 
GCTGAAGCTG ATGCTTTGGC TGATGCTGAG GAAAGGTGTG ACCAGCTCAT CAAAACCAAA 
ATCCAGCTGG AAGCCAAAAT TAAGGAGGTG ACAGAACGGG CTGAGGATGA GGAGGAAATT 
AATGCTGAAC TGACAGCCAA GAAGAGGAAA CTGGAGGATG AATGCTCAGA GCTGAAGAAA 
GATATAGATG ACCTGGAGTT AACATTGGCC AAAGTTGAGA AGGAAAAGCA TGCCACTGAA 
AACAAGGTGA AAAACCTCAC AGAAGAGATG GCAGCCCTGG ATGAGACAAT TGCCAAGCTG 
ACAAAAGAGA AGAAAGCCCT CCAAGAGGCC CATCAGCAGA CACTGGATGA CCTGCAGGCT 
GAAGAGGACA AAGTCAATAC GCTGACCAAA GCTAAAACCA AGCTGGAACA GCAAGTGGAC 
GATCTGGAAG GGTCCCTGGA GCAAGAGAAG AAGCTGCGCA TGGACCTCGA GAGAGCTAAG 
AGGAAACTTG AAGGAGACCT GAAGATGTCC CAGGATACCA TAATGGATTT GGAAAATGAT 
AAACAGCAGC TGGATGAGAA ACTGAAGAAG AAAGACTTTG AAATCAGCCA GATCCAGAGC 
AAAATTGAGG ATGAGCAAGC CCTGGGCATG CAGTTACAGA AGAAGATCAA GGAGCTGCAG 
GCCCGTATTG AGGAACTGGA GGAGGAAATC GAGGCAGAGC GAACCTCTCG GGCAAAAGCA 
GAGAAGCACC GAGCTGACCT CTCCAGGGAG CTGGAGGAGA TCAGTGAGCG CCTGGAGGAA 
GCAGGAGGGG CTACAGCAGC TCAGATTGAT ATGAACAAGA AGCGTGAGGC AGAATTTCAG 
AAGATGCGCC GTGACCTCGA AGAGGCCACG CTGCAGCACG AAGCCACGGC TGCTGCCCTG 
CGGAAGAAGC ATGCGGACAG CACAGCGGAG CTTGGGGAGC AGATCGACAA CCTGCAACGT 
GTCAAGCAGA AGCTGGAGAA GGAGAAGAGT GAGCTGAAGA TGGAGATTGA TGACTTGGCC 
AGCAACATGG AGTCTGTCTC CAAAGCTAAG GCAAACCTGG AAAAGATGTG TCGTACTTTA 
GAAGACCAAC TGAGTGAGAT TAAGTCAAAG GAGGAGGAGC ATCAACGCAT GATAAACGAT 
CTCAGCACTC AAAGAGCTCG TCTGCAGACA GAATCAGGTG AATATTCACG CCAGGTGGAG 
GAGAAAGATG CCCTGATATC TCAGCTGTCT CGAGGCAAGC AGGCATTCAC CCAACAGATT 
GAGGAACTCA AGCGGCACTT AGAGGAAGAA ATCAAGGCCA AGAACGCCCT GGCCCACGCC 
TTGCAGTCTG CTCGTCATGA CTGTGATTTG CTCCGGGAAC AATATGAAGA GGAGCAGGAA 
GCCAAGGGTG AGCTGCAGCG TGCCCTGTCC AAGGCCAACA GTGAAGTGGC CCAGTGGAGA 
ACCAAATATG AGACAGATGC TATTCAGCGC ACAGAGGAAC TGGAGGAGGC CAAGAAGAAG 
CTGGCACAGC GCCTGCAGGA TGCCGAGGAA CATGTTGAAG CTGTGAATGC CAAATGTGCT 
TCCCTGGAAA AGACAAAGCA GAGGCTGCAG AATGAGGTGG AGGACCTGAT GATTGACGTG 
GAGCGAGCAA ATGCTGCCTG CGCAGCTCTG GACAAGAAGC AGAAGAACTT TGACAAGATC 
CTGGCAGAGT GGAAGCAGAA GTACGAGGAA ACGCAGGCTG AGCTGGAGGC CTCCCAGAAG 
GAGTCTCGCT CCCTCAGCAC AGAGCTGTTT AAGATGAAGA ATGCCTATGA GGAATCCCTG 
GACCACCTGG AAACGCTGAA GCGTGAGAAC AAGAACTTGC AGCAGGAGAT TTCTGACCTC 
ACGGAGCAGA TTGCAGAGGG AGGAAAGGCA ATTCATGAGT TGGAGAAAGT CAAGAAGCAG 
ATTGAGCAGG AGAAGTCTGA AATCCAGGCT GCTCTGGAAG AAGCTGAGGC CTCCCTGGAA 
CATGAAGAGG GGAAGATCCT GCGCCTCCAA CTGGAGCTCA ACCAGGTCAA GTCTGAGATT 
GACAGGAAAA TAGCAGAGAA AGATGAGGAA ATTGACCAGC TGAAGAGAAA TCACCTCAGA 
ATTGTGGAGT CCATGCAGAG CACTCTGGAT GCTGAGATCA GGAGCAGGAA TGAAGCCCTT 
AGACTGAAGA AGAAGATGGA GGGAGACCTG AATGAAATGG AGATCCAGCT CAACCATGCC 
AACCGCGTGG CTGCAGAGGC ACAAAAGAAC CTTAGAAACA CACAAGGAGT GCTCAAAGAT 
ACCCAGATAC ACTTGGATGA TGCTCTCAGG ACACAGGAGG ACCTGAAGGA GCAGGTGGCC 
ATGGTGGAGC GCAGAGCAAA CCTGTTGCAG GCTGAGATTG AGGAGCTACG GGCAGCCCTG 
GAACAGACAG AGCGCTCGAG AAAAGTGGCT GAGCAGGAAT TGATGGATGC AAGTGAGAGA 
GTTCAGCTCC TCCACACTCA GAACACCAGC TTGATCAACA CCAAGAAGAA ACTGGAAACA 
GACATTGCCC AAATTCAGAG TGAAATGGAG GATACCATCC AGGAAGCCCG CAATGCTGAA 
GAGAAGGCCA AGAAGGCCAT CACCGATGCA GCCATGATGG CAGAAGAGCT GAAGAAGGAG 
CAGGACACCA GTGCCCACCT GGAGAGGATG AAGAAGAACC TGGACCAGAC GGTGAAGGAT 
CTGCAGCTCC GTCTGGATGA GGCAGAGCAG CTGGCCCTGA AGGGAGGCAA GAAGCAAATT 
CAGAAGCTGG AGGCCAGGGT GCGGGAGCTG GAAGGGGAGG TTGATGCTGA GCAGAAGCGC 
AGCGCTGAAG CCGTGAAGGG TGTGCGCAAG TACGAGAGGA GGGTGAAGGA GCTGACATAC 
CAGTCTGAGG AAGATCGCAA GAACATTCTT AGACTCCAGG ATCTGGTGGA CAAACTGCAG 
ATGAAGGTGA AATCCTACAA GAGACAAGCT GAGGAGGCTG AGGAGCTGTC CAATGTCAAC 
CTCACCAAGT TCCGCAAGAT CCAGCATGAG CTGGAGGAAG CCGAGGAGCG GGCTGACATT 
GCAGAGTCAC AGGTCAACAA GCTCCGAGCA AAGAGCCGGG AGTTTCATGG CAAGAAGATA 
GAAGAGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

EMX2 activates slow myosin heavy chain 2 gene expression in embryonic muscle fibers.
Mechanisms of development1478-16(2017 Oct)
Hatch K,Pabon A,DiMario JX

Muscle fiber type specific induction of slow myosin heavy chain 2 gene expression by electrical stimulation.
Experimental cell research316(6)1039-49(2010 Apr)
Crew JR,Falzari K,DiMario JX

Betaine protects urea-induced denaturation of myosin subfragment-1.
The FEBS journal275(13)3388-96(2008 Jul)
Ortiz-Costa S,Sorenson MM,Sola-Penna M

Conserved Asp-137 imparts flexibility to tropomyosin and affects function.
The Journal of biological chemistry283(11)6728-34(2008 Mar)
Sumida JP,Wu E,Lehrer SS

Evidence for the expression of neonatal skeletal myosin heavy chain in primary myocardium and cardiac conduction tissue in the developing chick heart.
Developmental dynamics : an official publication of the American Association of Anatomists217(1)37-49(2000 Jan)
Machida S,Matsuoka R,Noda S,Hiratsuka E,Takagaki Y,Oana S,Furutani Y,Nakajima H,Takao A,Momma K

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MYH1B cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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