KCNMA1 cDNA ORF clone, Gallus gallus(chicken)

The following KCNMA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KCNMA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa37463 XM_015288191.1
Latest version!
Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X44, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OGa151410 XM_025151434.1
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa151411 XM_025151436.1
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa151412 XM_025151438.1
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X37, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa151413 XM_025151439.1
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X38, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa151414 XM_025151440.1
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X41, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa151415 XM_025151442.1
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X44, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OGa151416 XM_025151443.1
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X46, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OGa37420 XM_015288147.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37421 XM_015288148.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37422 XM_015288149.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37423 XM_015288150.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37424 XM_015288151.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37425 XM_015288152.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37426 XM_015288153.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37427 XM_015288154.1
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37428 XM_015288155.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37429 XM_015288156.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37430 XM_015288157.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37431 XM_015288158.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37432 XM_015288159.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37433 XM_015288160.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37434 XM_015288161.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37435 XM_015288162.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37436 XM_015288163.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37437 XM_015288164.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37438 XM_015288165.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37439 XM_015288166.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37440 XM_015288167.1
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37441 XM_015288168.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37442 XM_015288169.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37443 XM_015288170.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37444 XM_015288171.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37445 XM_015288172.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37446 XM_015288173.1
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37447 XM_015288174.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X30, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37448 XM_015288175.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X31, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37449 XM_015288176.1
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X32, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37450 XM_015288177.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X33, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37451 XM_015288178.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X34, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37452 XM_015288179.1
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X35, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37453 XM_015288180.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X36, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37454 XM_015288181.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X39, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37455 XM_015288182.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X40, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37456 XM_015288183.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X42, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37457 XM_015288184.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X43, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa37458 XM_015288185.1
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X45, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa37459 XM_015288186.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X47, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa37460 XM_015288187.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X48, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa37461 XM_015288189.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X49, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa37462 XM_015288190.2
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X50, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa27550 NM_204224.1
Latest version!
Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OGa37439 XM_015288166.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37422 XM_015288149.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37429 XM_015288156.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37442 XM_015288169.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37455 XM_015288182.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X36, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37426 XM_015288153.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37448 XM_015288175.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37462 XM_015288190.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X43, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa37433 XM_015288160.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37434 XM_015288161.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37443 XM_015288170.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37451 XM_015288178.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X32, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37460 XM_015288187.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X41, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa37432 XM_015288159.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37436 XM_015288163.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37450 XM_015288177.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X31, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37454 XM_015288181.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X35, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37457 XM_015288184.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X38, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa37459 XM_015288186.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X40, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa37423 XM_015288150.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37456 XM_015288183.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X37, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37420 XM_015288147.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37437 XM_015288164.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37444 XM_015288171.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37453 XM_015288180.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X34, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37421 XM_015288148.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37424 XM_015288151.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37425 XM_015288152.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37428 XM_015288155.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37430 XM_015288157.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37431 XM_015288158.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37435 XM_015288162.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37438 XM_015288165.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37441 XM_015288168.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37445 XM_015288172.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37447 XM_015288174.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa37461 XM_015288189.1 Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X42, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa37463
    Clone ID Related Accession (Same CDS sequence) XM_015288191.1
    Accession Version XM_015288191.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2841bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X44
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTTTGCTC GCTACGTGCC AGAAATTGCT GCTCTCATCC TTAATCGGAA GAAATACGGC 
    GGGACTTTTA ACTCAACCCG AGGCAGAAAG CACATAGTTG TCTGTGGTCA CATAACACTT
    GAAAGCGTGT CCAACTTCCT GAAGGACTTC CTGCACAAGG ATAGGGATGA TGTTAACGTA
    GAAATCGTCT TTCTGCATAA TATCTCCCCT AACCTTGAGC TGGAAGCTCT TTTTAAACGA
    CACTTCACTC AGGTGGAATT TTATCAGGGT TCAGTCCTTA ATCCCCATGA CCTGGCAAGA
    GTAAAGATAG AGTCAGCAGA TGCGTGCCTA ATTCTTGCCA ATAAATACTG CGCTGACCCA
    GATGCTGAGG ATGCCTCCAA TATTATGAGA GTTATTTCAA TAAAGAATTA TCATCCGAAG
    ATAAGAATTA TCACTCAGAT GTTGCAGTAT CACAATAAGG CTCATCTGTT AAATATTCCA
    AGCTGGAATT GGAAGGAAGG GGATGATGCG ATCTGTCTTG CTGAGCTCAA GCTGGGTTTC
    ATAGCTCAGA GTTGCCTGGC ACCAGGCCTT TCAACAATGT TAGCCAACCT CTTTTCCATG
    AGATCATTCA TAAAGATTGA GGAGGATACA TGGCAGAAAT ACTATCTGGA AGGAGTTGCA
    AATGAAATGT ATACAGAATA TCTGTCTAGT GCCTTTGTGG GTTTGTCCTT CCCTGCAGTT
    TGTGAACTGG TGTTTGCGAA ACTAAAGCTC TTAATGATAG CCATAGAATA CAAGTCGGAA
    AAAAGAGAAA GCAGAAGCCG AAAGCGTATA TTGATCAATC CTGGAAACCA TGTGAAGATT
    CAAGAAGGTA CCTTAGGATT CTTCATTGCA AGTGATGCCA AAGAAGTAAA AAGGGCATTT
    TTTTACTGCA AGGCCTGCCA TGATGACATC ACAGATCCCA AAAGGATAAA AAAGTGTGGC
    TGCAAACGGC TGATCTATTC CAAGATGTCC ATCTACAAGA GAATGAAACT GGCATGTTGT
    TTTGATTGCG GACGCTCTGA GCGTGACTGC TCTTGCATGT CAGGCAGTGT ACATAGTAAC
    ATGGACACCC TTGAGAGAGC CTTCCCACTT TCTTCTGTCT CTGTTAATGA TTGCTCCACC
    AGTTTACGTG CCTTTGAAGA TGAGCAGCCG TCAACACTAT CACCCAAAAA AAAGCAACGA
    AATGGAGGCA TGCGAAATTC ACCCAACTCC TCACCCAAAC TGATGAGGCA TGACCCCTTG
    TTAATTCCTG GCAATGAACA GATTGACAAT ATGGATGCCA ATGTGAAAAA ATATGACTCT
    ACGGGAATGT TTCATTGGTG TCCAGCCAAG GATATTGAAA AGGTCATTTT GACTCGAAGT
    GAAGCAGCGA TGACAGTTTT AAGTGGGCAT GTAGTCGTTT GCATATTTGG GGATGTTAAA
    TCTGCTTTGA TTGGATTAAG AAATTTAGTG ATGCCATTAC GAGCCAGCAA CTTTCACTAC
    CATGAACTCA AGCACATTGT GTTTGTGGGT TCACTTGAAT ACCTGAGAAG GGAATGGGAG
    ACCCTGCATA ACTTCCCCAA GGTTTCAATC TTGCCTGGTA CACCATTAAG TCGGGCTGAT
    TTAAGGGCTG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT
    AATATTGATG ATGCTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA
    TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG CTTTACACCC
    CCTGGTATGG ATAGGTCATC TCCAGACAAC AGTCCTGTCC ACGGCCTGTT ACGTCAACCC
    TCCATTACAA CAGGAGCCAA CATCCCTATT ATAACAGAGC TAGCTAAGCC TGGCAAACTT
    CTGCCTTTGG TTTCAATCAG TCAGGAAAAA AACAGTGGAA CCCACATTCT AATGATAACT
    GAACTGGTAA ATGATTCAAA TGTTCAGTTC TTGGACCAAG ATGATGATGA TGATCCAGAT
    ACAGAACTGT ACCTCACGCA GCCCTTTGCA TGTGGAACCG CATTTGCTGT CAGCGTGCTG
    GACTCTCTCA TGAGCGCAAC ATACTTCAAT GACAATATCC TCACACTGAT ACGGACATTG
    GTAACAGGAG GAGCCACACC AGAGCTGGAA GCACTGATTG CCGAGGAAAA TGCTTTGAGA
    GGAGGTTACA GCACGCCCCA GACACTTGCA AACAGGGACA GGTGTCGAGT TGCTCAGTTG
    GCTTTGTATG ATGGGCCATT TGCAGACCTA GGGGATGGAG GCTGCTATGG AGATCTATTT
    TGTAAAGCAC TGAAAACATA CAATATGCTT TGCTTTGGAA TTTATCGATT AAGGGACGCT
    CATCTAAGTA CTCCCAGCCA ATGCACTAAA CGTTATGTTA TCACAAACCC ACCATATGAG
    TTTGAGCTTG TGCCGACAGA CTTGATCTTC TGTTTGATGC AATTCGACCA CAATGCTGGC
    CAGTCCCGGG CCAGTCTTTC TCATTCCTCC CATTCCTCCT ATTCTTCCAG CAAGAAGAGC
    TCATCAGTTC ACTCCATCCC ATCAACAGCA AACCGACCGA ACCGCACCAA GACCAGGGAT
    TCCCGGGAAA AACAGAATGC AACAAGGATG AATAGAATGG GCCAAGCAGA AAAGAAATGG
    TTTACAGATG AACCGGATAA TGCCTATCCC AGAAACATTC AAATCAAGCC CATGAGCACC
    CACATGGCCA ATCAGATCAA TCAATACAAA TCGACAAGCA GCTTGATACC ACCAATCAGA
    GAAGTTGAAG ATGAATGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143677.1
    CDS21..2861
    Translation

    Target ORF information:

    RefSeq Version XM_015288191.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X44, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288191.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTTTGCTC GCTACGTGCC AGAAATTGCT GCTCTCATCC TTAATCGGAA GAAATACGGC 
    GGGACTTTTA ACTCAACCCG AGGCAGAAAG CACATAGTTG TCTGTGGTCA CATAACACTT
    GAAAGCGTGT CCAACTTCCT GAAGGACTTC CTGCACAAGG ATAGGGATGA TGTTAACGTA
    GAAATCGTCT TTCTGCATAA TATCTCCCCT AACCTTGAGC TGGAAGCTCT TTTTAAACGA
    CACTTCACTC AGGTGGAATT TTATCAGGGT TCAGTCCTTA ATCCCCATGA CCTGGCAAGA
    GTAAAGATAG AGTCAGCAGA TGCGTGCCTA ATTCTTGCCA ATAAATACTG CGCTGACCCA
    GATGCTGAGG ATGCCTCCAA TATTATGAGA GTTATTTCAA TAAAGAATTA TCATCCGAAG
    ATAAGAATTA TCACTCAGAT GTTGCAGTAT CACAATAAGG CTCATCTGTT AAATATTCCA
    AGCTGGAATT GGAAGGAAGG GGATGATGCG ATCTGTCTTG CTGAGCTCAA GCTGGGTTTC
    ATAGCTCAGA GTTGCCTGGC ACCAGGCCTT TCAACAATGT TAGCCAACCT CTTTTCCATG
    AGATCATTCA TAAAGATTGA GGAGGATACA TGGCAGAAAT ACTATCTGGA AGGAGTTGCA
    AATGAAATGT ATACAGAATA TCTGTCTAGT GCCTTTGTGG GTTTGTCCTT CCCTGCAGTT
    TGTGAACTGG TGTTTGCGAA ACTAAAGCTC TTAATGATAG CCATAGAATA CAAGTCGGAA
    AAAAGAGAAA GCAGAAGCCG AAAGCGTATA TTGATCAATC CTGGAAACCA TGTGAAGATT
    CAAGAAGGTA CCTTAGGATT CTTCATTGCA AGTGATGCCA AAGAAGTAAA AAGGGCATTT
    TTTTACTGCA AGGCCTGCCA TGATGACATC ACAGATCCCA AAAGGATAAA AAAGTGTGGC
    TGCAAACGGC TGATCTATTC CAAGATGTCC ATCTACAAGA GAATGAAACT GGCATGTTGT
    TTTGATTGCG GACGCTCTGA GCGTGACTGC TCTTGCATGT CAGGCAGTGT ACATAGTAAC
    ATGGACACCC TTGAGAGAGC CTTCCCACTT TCTTCTGTCT CTGTTAATGA TTGCTCCACC
    AGTTTACGTG CCTTTGAAGA TGAGCAGCCG TCAACACTAT CACCCAAAAA AAAGCAACGA
    AATGGAGGCA TGCGAAATTC ACCCAACTCC TCACCCAAAC TGATGAGGCA TGACCCCTTG
    TTAATTCCTG GCAATGAACA GATTGACAAT ATGGATGCCA ATGTGAAAAA ATATGACTCT
    ACGGGAATGT TTCATTGGTG TCCAGCCAAG GATATTGAAA AGGTCATTTT GACTCGAAGT
    GAAGCAGCGA TGACAGTTTT AAGTGGGCAT GTAGTCGTTT GCATATTTGG GGATGTTAAA
    TCTGCTTTGA TTGGATTAAG AAATTTAGTG ATGCCATTAC GAGCCAGCAA CTTTCACTAC
    CATGAACTCA AGCACATTGT GTTTGTGGGT TCACTTGAAT ACCTGAGAAG GGAATGGGAG
    ACCCTGCATA ACTTCCCCAA GGTTTCAATC TTGCCTGGTA CACCATTAAG TCGGGCTGAT
    TTAAGGGCTG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT
    AATATTGATG ATGCTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA
    TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG CTTTACACCC
    CCTGGTATGG ATAGGTCATC TCCAGACAAC AGTCCTGTCC ACGGCCTGTT ACGTCAACCC
    TCCATTACAA CAGGAGCCAA CATCCCTATT ATAACAGAGC TAGCTAAGCC TGGCAAACTT
    CTGCCTTTGG TTTCAATCAG TCAGGAAAAA AACAGTGGAA CCCACATTCT AATGATAACT
    GAACTGGTAA ATGATTCAAA TGTTCAGTTC TTGGACCAAG ATGATGATGA TGATCCAGAT
    ACAGAACTGT ACCTCACGCA GCCCTTTGCA TGTGGAACCG CATTTGCTGT CAGCGTGCTG
    GACTCTCTCA TGAGCGCAAC ATACTTCAAT GACAATATCC TCACACTGAT ACGGACATTG
    GTAACAGGAG GAGCCACACC AGAGCTGGAA GCACTGATTG CCGAGGAAAA TGCTTTGAGA
    GGAGGTTACA GCACGCCCCA GACACTTGCA AACAGGGACA GGTGTCGAGT TGCTCAGTTG
    GCTTTGTATG ATGGGCCATT TGCAGACCTA GGGGATGGAG GCTGCTATGG AGATCTATTT
    TGTAAAGCAC TGAAAACATA CAATATGCTT TGCTTTGGAA TTTATCGATT AAGGGACGCT
    CATCTAAGTA CTCCCAGCCA ATGCACTAAA CGTTATGTTA TCACAAACCC ACCATATGAG
    TTTGAGCTTG TGCCGACAGA CTTGATCTTC TGTTTGATGC AATTCGACCA CAATGCTGGC
    CAGTCCCGGG CCAGTCTTTC TCATTCCTCC CATTCCTCCT ATTCTTCCAG CAAGAAGAGC
    TCATCAGTTC ACTCCATCCC ATCAACAGCA AACCGACCGA ACCGCACCAA GACCAGGGAT
    TCCCGGGAAA AACAGAATGC AACAAGGATG AATAGAATGG GCCAAGCAGA AAAGAAATGG
    TTTACAGATG AACCGGATAA TGCCTATCCC AGAAACATTC AAATCAAGCC CATGAGCACC
    CACATGGCCA ATCAGATCAA TCAATACAAA TCGACAAGCA GCTTGATACC ACCAATCAGA
    GAAGTTGAAG ATGAATGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa151410
    Clone ID Related Accession (Same CDS sequence) XM_025151434.1
    Accession Version XM_025151434.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3663bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GTAAATGATT CAAATGTTCA GTTCTTGGAC
    CAAGATGATG ATGATGATCC AGATACAGAA CTGTACCTCA CGCAGCCCTT TGCATGTGGA
    ACCGCATTTG CTGTCAGCGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT
    ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG
    ATTGCCGAGG AAAATGCTTT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG
    GACAGGTGTC GAGTTGCTCA GTTGGCTTTG TATGATGGGC CATTTGCAGA CCTAGGGGAT
    GGAGGCTGCT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT
    GGAATTTATC GATTAAGGGA CGCTCATCTA AGTACTCCCA GCCAATGCAC TAAACGTTAT
    GTTATCACAA ACCCACCATA TGAGTTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG
    ATGCAATTCG ACCACAATGC TGGCCAGTCC CGGGCCAGTC TTTCTCATTC CTCCCATTCC
    TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCCA TCCCATCAAC AGCAAACCGA
    CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA AAAAAGAAAT GGTTTACAGA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025007202.1
    CDS368..4030
    Translation

    Target ORF information:

    RefSeq Version XM_025151434.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025151434.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GTAAATGATT CAAATGTTCA GTTCTTGGAC
    CAAGATGATG ATGATGATCC AGATACAGAA CTGTACCTCA CGCAGCCCTT TGCATGTGGA
    ACCGCATTTG CTGTCAGCGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT
    ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG
    ATTGCCGAGG AAAATGCTTT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG
    GACAGGTGTC GAGTTGCTCA GTTGGCTTTG TATGATGGGC CATTTGCAGA CCTAGGGGAT
    GGAGGCTGCT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT
    GGAATTTATC GATTAAGGGA CGCTCATCTA AGTACTCCCA GCCAATGCAC TAAACGTTAT
    GTTATCACAA ACCCACCATA TGAGTTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG
    ATGCAATTCG ACCACAATGC TGGCCAGTCC CGGGCCAGTC TTTCTCATTC CTCCCATTCC
    TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCCA TCCCATCAAC AGCAAACCGA
    CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA AAAAAGAAAT GGTTTACAGA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa151411
    Clone ID Related Accession (Same CDS sequence) XM_025151436.1
    Accession Version XM_025151436.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X28
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTT
    GAAGATGAGC AGCCGTCAAC ACTATCACCC AAAAAAAAGC AACGAAATGG AGGCATGCGA
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAT
    GAACAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACGGG AATGTTTCAT
    TGGTGTCCAG CCAAGGATAT TGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACCCT GCATAACTTC
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGCTTTA CACCCCCTGG TATGGATAGG
    TCATCTCCAG ACAACAGTCC TGTCCACGGC CTGTTACGTC AACCCTCCAT TACAACAGGA
    GCCAACATCC CTATTATAAC AGAGCTAGTA AATGATTCAA ATGTTCAGTT CTTGGACCAA
    GATGATGATG ATGATCCAGA TACAGAACTG TACCTCACGC AGCCCTTTGC ATGTGGAACC
    GCATTTGCTG TCAGCGTGCT GGACTCTCTC ATGAGCGCAA CATACTTCAA TGACAATATC
    CTCACACTGA TACGGACATT GGTAACAGGA GGAGCCACAC CAGAGCTGGA AGCACTGATT
    GCCGAGGAAA ATGCTTTGAG AGGAGGTTAC AGCACGCCCC AGACACTTGC AAACAGGGAC
    AGGTGTCGAG TTGCTCAGTT GGCTTTGTAT GATGGGCCAT TTGCAGACCT AGGGGATGGA
    GGCTGCTATG GAGATCTATT TTGTAAAGCA CTGAAAACAT ACAATATGCT TTGCTTTGGA
    ATTTATCGAT TAAGGGACGC TCATCTAAGT ACTCCCAGCC AATGCACTAA ACGTTATGTT
    ATCACAAACC CACCATATGA GTTTGAGCTT GTGCCGACAG ACTTGATCTT CTGTTTGATG
    CAATTCGACC ACAATGCTGG CCAGTCCCGG GCCAGTCTTT CTCATTCCTC CCATTCCTCC
    TATTCTTCCA GCAAGAAGAG CTCATCAGTT CACTCCATCC CATCAACAGC AAACCGACCG
    AACCGCACCA AGACCAGGGA TTCCCGGGAA AAACAGAATG CAACAAGGAT GAATAGAATG
    GGCCAAGCAG AAAAGAAATG GTTTACAGAT GAACCGGATA ATGCCTATCC CAGAAACATT
    CAAATCAAGC CCATGAGCAC CCACATGGCC AATCAGATCA ATCAATACAA ATCGACAAGC
    AGCTTGATAC CACCAATCAG AGAAGTTGAA GATGAATGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025007204.1
    CDS367..4008
    Translation

    Target ORF information:

    RefSeq Version XM_025151436.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025151436.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTT
    GAAGATGAGC AGCCGTCAAC ACTATCACCC AAAAAAAAGC AACGAAATGG AGGCATGCGA
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAT
    GAACAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACGGG AATGTTTCAT
    TGGTGTCCAG CCAAGGATAT TGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACCCT GCATAACTTC
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGCTTTA CACCCCCTGG TATGGATAGG
    TCATCTCCAG ACAACAGTCC TGTCCACGGC CTGTTACGTC AACCCTCCAT TACAACAGGA
    GCCAACATCC CTATTATAAC AGAGCTAGTA AATGATTCAA ATGTTCAGTT CTTGGACCAA
    GATGATGATG ATGATCCAGA TACAGAACTG TACCTCACGC AGCCCTTTGC ATGTGGAACC
    GCATTTGCTG TCAGCGTGCT GGACTCTCTC ATGAGCGCAA CATACTTCAA TGACAATATC
    CTCACACTGA TACGGACATT GGTAACAGGA GGAGCCACAC CAGAGCTGGA AGCACTGATT
    GCCGAGGAAA ATGCTTTGAG AGGAGGTTAC AGCACGCCCC AGACACTTGC AAACAGGGAC
    AGGTGTCGAG TTGCTCAGTT GGCTTTGTAT GATGGGCCAT TTGCAGACCT AGGGGATGGA
    GGCTGCTATG GAGATCTATT TTGTAAAGCA CTGAAAACAT ACAATATGCT TTGCTTTGGA
    ATTTATCGAT TAAGGGACGC TCATCTAAGT ACTCCCAGCC AATGCACTAA ACGTTATGTT
    ATCACAAACC CACCATATGA GTTTGAGCTT GTGCCGACAG ACTTGATCTT CTGTTTGATG
    CAATTCGACC ACAATGCTGG CCAGTCCCGG GCCAGTCTTT CTCATTCCTC CCATTCCTCC
    TATTCTTCCA GCAAGAAGAG CTCATCAGTT CACTCCATCC CATCAACAGC AAACCGACCG
    AACCGCACCA AGACCAGGGA TTCCCGGGAA AAACAGAATG CAACAAGGAT GAATAGAATG
    GGCCAAGCAG AAAAGAAATG GTTTACAGAT GAACCGGATA ATGCCTATCC CAGAAACATT
    CAAATCAAGC CCATGAGCAC CCACATGGCC AATCAGATCA ATCAATACAA ATCGACAAGC
    AGCTTGATAC CACCAATCAG AGAAGTTGAA GATGAATGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa151412
    Clone ID Related Accession (Same CDS sequence) XM_025151438.1
    Accession Version XM_025151438.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X37
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTTTG AAGATGAGCA GCCGTCAACA CTATCACCCA AAAAAAAGCA ACGAAATGGA
    GGCATGCGAA ATTCACCCAA CTCCTCACCC AAACTGATGA GGCATGACCC CTTGTTAATT
    CCTGGCAATG AACAGATTGA CAATATGGAT GCCAATGTGA AAAAATATGA CTCTACGGGA
    ATGTTTCATT GGTGTCCAGC CAAGGATATT GAAAAGGTCA TTTTGACTCG AAGTGAAGCA
    GCGATGACAG TTTTAAGTGG GCATGTAGTC GTTTGCATAT TTGGGGATGT TAAATCTGCT
    TTGATTGGAT TAAGAAATTT AGTGATGCCA TTACGAGCCA GCAACTTTCA CTACCATGAA
    CTCAAGCACA TTGTGTTTGT GGGTTCACTT GAATACCTGA GAAGGGAATG GGAGACCCTG
    CATAACTTCC CCAAGGTTTC AATCTTGCCT GGTACACCAT TAAGTCGGGC TGATTTAAGG
    GCTGTCAACA TCAACCTCTG CGACATGTGC GTTATCCTGT CAGCCAATCA GAATAATATT
    GATGATGCTT CGCTGCAGGA CAAGGAATGC ATCTTGGCGT CACTCAACAT CAAATCTATG
    CAGTTTGATG ACAGCATTGG GGTCTTGCAG GCTAATTCCC AAGGCTTTAC ACCCCCTGGT
    ATGGATAGGT CATCTCCAGA CAACAGTCCT GTCCACGGCC TGTTACGTCA ACCCTCCATT
    ACAACAGGAG CCAACATCCC TATTATAACA GAGCTAGCTA AGCCTGGCAA ACTTCTGCCT
    TTGGTTTCAA TCAGTCAGGA AAAAAACAGT GGAACCCACA TTCTAATGAT AACTGAACTG
    GTAAATGATT CAAATGTTCA GTTCTTGGAC CAAGATGATG ATGATGATCC AGATACAGAA
    CTGTACCTCA CGCAGCCCTT TGCATGTGGA ACCGCATTTG CTGTCAGCGT GCTGGACTCT
    CTCATGAGCG CAACATACTT CAATGACAAT ATCCTCACAC TGATACGGAC ATTGGTAACA
    GGAGGAGCCA CACCAGAGCT GGAAGCACTG ATTGCCGAGG AAAATGCTTT GAGAGGAGGT
    TACAGCACGC CCCAGACACT TGCAAACAGG GACAGGTGTC GAGTTGCTCA GTTGGCTTTG
    TATGATGGGC CATTTGCAGA CCTAGGGGAT GGAGGCTGCT ATGGAGATCT ATTTTGTAAA
    GCACTGAAAA CATACAATAT GCTTTGCTTT GGAATTTATC GATTAAGGGA CGCTCATCTA
    AGTACTCCCA GCCAATGCAC TAAACGTTAT GTTATCACAA ACCCACCATA TGAGTTTGAG
    CTTGTGCCGA CAGACTTGAT CTTCTGTTTG ATGCAATTCG ACCACAATGC TGGCCAGTCC
    CGGGCCAGTC TTTCTCATTC CTCCCATTCC TCCTATTCTT CCAGCAAGAA GAGCTCATCA
    GTTCACTCCA TCCCATCAAC AGCAAACCGA CCGAACCGCA CCAAGACCAG GGATTCCCGG
    GAAAAACAGA ACAGAAAAGA AATGGTTTAC AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025007206.1
    CDS368..3943
    Translation

    Target ORF information:

    RefSeq Version XM_025151438.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X37, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025151438.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTTTG AAGATGAGCA GCCGTCAACA CTATCACCCA AAAAAAAGCA ACGAAATGGA
    GGCATGCGAA ATTCACCCAA CTCCTCACCC AAACTGATGA GGCATGACCC CTTGTTAATT
    CCTGGCAATG AACAGATTGA CAATATGGAT GCCAATGTGA AAAAATATGA CTCTACGGGA
    ATGTTTCATT GGTGTCCAGC CAAGGATATT GAAAAGGTCA TTTTGACTCG AAGTGAAGCA
    GCGATGACAG TTTTAAGTGG GCATGTAGTC GTTTGCATAT TTGGGGATGT TAAATCTGCT
    TTGATTGGAT TAAGAAATTT AGTGATGCCA TTACGAGCCA GCAACTTTCA CTACCATGAA
    CTCAAGCACA TTGTGTTTGT GGGTTCACTT GAATACCTGA GAAGGGAATG GGAGACCCTG
    CATAACTTCC CCAAGGTTTC AATCTTGCCT GGTACACCAT TAAGTCGGGC TGATTTAAGG
    GCTGTCAACA TCAACCTCTG CGACATGTGC GTTATCCTGT CAGCCAATCA GAATAATATT
    GATGATGCTT CGCTGCAGGA CAAGGAATGC ATCTTGGCGT CACTCAACAT CAAATCTATG
    CAGTTTGATG ACAGCATTGG GGTCTTGCAG GCTAATTCCC AAGGCTTTAC ACCCCCTGGT
    ATGGATAGGT CATCTCCAGA CAACAGTCCT GTCCACGGCC TGTTACGTCA ACCCTCCATT
    ACAACAGGAG CCAACATCCC TATTATAACA GAGCTAGCTA AGCCTGGCAA ACTTCTGCCT
    TTGGTTTCAA TCAGTCAGGA AAAAAACAGT GGAACCCACA TTCTAATGAT AACTGAACTG
    GTAAATGATT CAAATGTTCA GTTCTTGGAC CAAGATGATG ATGATGATCC AGATACAGAA
    CTGTACCTCA CGCAGCCCTT TGCATGTGGA ACCGCATTTG CTGTCAGCGT GCTGGACTCT
    CTCATGAGCG CAACATACTT CAATGACAAT ATCCTCACAC TGATACGGAC ATTGGTAACA
    GGAGGAGCCA CACCAGAGCT GGAAGCACTG ATTGCCGAGG AAAATGCTTT GAGAGGAGGT
    TACAGCACGC CCCAGACACT TGCAAACAGG GACAGGTGTC GAGTTGCTCA GTTGGCTTTG
    TATGATGGGC CATTTGCAGA CCTAGGGGAT GGAGGCTGCT ATGGAGATCT ATTTTGTAAA
    GCACTGAAAA CATACAATAT GCTTTGCTTT GGAATTTATC GATTAAGGGA CGCTCATCTA
    AGTACTCCCA GCCAATGCAC TAAACGTTAT GTTATCACAA ACCCACCATA TGAGTTTGAG
    CTTGTGCCGA CAGACTTGAT CTTCTGTTTG ATGCAATTCG ACCACAATGC TGGCCAGTCC
    CGGGCCAGTC TTTCTCATTC CTCCCATTCC TCCTATTCTT CCAGCAAGAA GAGCTCATCA
    GTTCACTCCA TCCCATCAAC AGCAAACCGA CCGAACCGCA CCAAGACCAG GGATTCCCGG
    GAAAAACAGA ACAGAAAAGA AATGGTTTAC AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa151413
    Clone ID Related Accession (Same CDS sequence) XM_025151439.1
    Accession Version XM_025151439.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X38
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTT
    GAAGATGAGC AGCCGTCAAC ACTATCACCC AAAAAAAAGC AACGAAATGG AGGCATGCGA
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAT
    GAACAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACGGG AATGTTTCAT
    TGGTGTCCAG CCAAGGATAT TGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACCCT GCATAACTTC
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGCTTTA CACCCCCTGG TATGGATAGG
    TCATCTCCAG ACAACAGTCC TGTCCACGGC CTGTTACGTC AACCCTCCAT TACAACAGGA
    GCCAACATCC CTATTATAAC AGAGCTAGCT AAGCCTGGCA AACTTCTGCC TTTGGTTTCA
    ATCAGTCAGG AAAAAAACAG TGGAACCCAC ATTCTAATGA TAACTGAACT GGTAAATGAT
    TCAAATGTTC AGTTCTTGGA CCAAGATGAT GATGATGATC CAGATACAGA ACTGTACCTC
    ACGCAGCCCT TTGCATGTGG AACCGCATTT GCTGTCAGCG TGCTGGACTC TCTCATGAGC
    GCAACATACT TCAATGACAA TATCCTCACA CTGATACGGA CATTGGTAAC AGGAGGAGCC
    ACACCAGAGC TGGAAGCACT GATTGCCGAG GAAAATGCTT TGAGAGGAGG TTACAGCACG
    CCCCAGACAC TTGCAAACAG GGACAGGTGT CGAGTTGCTC AGTTGGCTTT GTATGATGGG
    CCATTTGCAG ACCTAGGGGA TGGAGGCTGC TATGGAGATC TATTTTGTAA AGCACTGAAA
    ACATACAATA TGCTTTGCTT TGGAATTTAT CGATTAAGGG ACGCTCATCT AAGTACTCCC
    AGCCAATGCA CTAAACGTTA TGTTATCACA AACCCACCAT ATGAGTTTGA GCTTGTGCCG
    ACAGACTTGA TCTTCTGTTT GATGCAATTC GACCACAATG CTGGCCAGTC CCGGGCCAGT
    CTTTCTCATT CCTCCCATTC CTCCTATTCT TCCAGCAAGA AGAGCTCATC AGTTCACTCC
    ATCCCATCAA CAGCAAACCG ACCGAACCGC ACCAAGACCA GGGATTCCCG GGAAAAACAG
    AAGTATGTGC AGGAAGATAG ACTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025007207.1
    CDS367..3933
    Translation

    Target ORF information:

    RefSeq Version XM_025151439.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X38, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025151439.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTT
    GAAGATGAGC AGCCGTCAAC ACTATCACCC AAAAAAAAGC AACGAAATGG AGGCATGCGA
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAT
    GAACAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACGGG AATGTTTCAT
    TGGTGTCCAG CCAAGGATAT TGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACCCT GCATAACTTC
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGCTTTA CACCCCCTGG TATGGATAGG
    TCATCTCCAG ACAACAGTCC TGTCCACGGC CTGTTACGTC AACCCTCCAT TACAACAGGA
    GCCAACATCC CTATTATAAC AGAGCTAGCT AAGCCTGGCA AACTTCTGCC TTTGGTTTCA
    ATCAGTCAGG AAAAAAACAG TGGAACCCAC ATTCTAATGA TAACTGAACT GGTAAATGAT
    TCAAATGTTC AGTTCTTGGA CCAAGATGAT GATGATGATC CAGATACAGA ACTGTACCTC
    ACGCAGCCCT TTGCATGTGG AACCGCATTT GCTGTCAGCG TGCTGGACTC TCTCATGAGC
    GCAACATACT TCAATGACAA TATCCTCACA CTGATACGGA CATTGGTAAC AGGAGGAGCC
    ACACCAGAGC TGGAAGCACT GATTGCCGAG GAAAATGCTT TGAGAGGAGG TTACAGCACG
    CCCCAGACAC TTGCAAACAG GGACAGGTGT CGAGTTGCTC AGTTGGCTTT GTATGATGGG
    CCATTTGCAG ACCTAGGGGA TGGAGGCTGC TATGGAGATC TATTTTGTAA AGCACTGAAA
    ACATACAATA TGCTTTGCTT TGGAATTTAT CGATTAAGGG ACGCTCATCT AAGTACTCCC
    AGCCAATGCA CTAAACGTTA TGTTATCACA AACCCACCAT ATGAGTTTGA GCTTGTGCCG
    ACAGACTTGA TCTTCTGTTT GATGCAATTC GACCACAATG CTGGCCAGTC CCGGGCCAGT
    CTTTCTCATT CCTCCCATTC CTCCTATTCT TCCAGCAAGA AGAGCTCATC AGTTCACTCC
    ATCCCATCAA CAGCAAACCG ACCGAACCGC ACCAAGACCA GGGATTCCCG GGAAAAACAG
    AAGTATGTGC AGGAAGATAG ACTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa151414
    Clone ID Related Accession (Same CDS sequence) XM_025151440.1
    Accession Version XM_025151440.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3564bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X41
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTT
    GAAGATGAGC AGCCGTCAAC ACTATCACCC AAAAAAAAGC AACGAAATGG AGGCATGCGA
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAT
    GAACAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACGGG AATGTTTCAT
    TGGTGTCCAG CCAAGGATAT TGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACCCT GCATAACTTC
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGCTTTA CACCCCCTGG TATGGATAGG
    TCATCTCCAG ACAACAGTCC TGTCCACGGC CTGTTACGTC AACCCTCCAT TACAACAGGA
    GCCAACATCC CTATTATAAC AGAGCTAGCT AAGCCTGGCA AACTTCTGCC TTTGGTTTCA
    ATCAGTCAGG AAAAAAACAG TGGAACCCAC ATTCTAATGA TAACTGAACT GGTAAATGAT
    TCAAATGTTC AGTTCTTGGA CCAAGATGAT GATGATGATC CAGATACAGA ACTGTACCTC
    ACGCAGCCCT TTGCATGTGG AACCGCATTT GCTGTCAGCG TGCTGGACTC TCTCATGAGC
    GCAACATACT TCAATGACAA TATCCTCACA CTGATACGGA CATTGGTAAC AGGAGGAGCC
    ACACCAGAGC TGGAAGCACT GATTGCCGAG GAAAATGCTT TGAGAGGAGG TTACAGCACG
    CCCCAGACAC TTGCAAACAG GGACAGGTGT CGAGTTGCTC AGTTGGCTTT GTATGATGGG
    CCATTTGCAG ACCTAGGGGA TGGAGGCTGC TATGGAGATC TATTTTGTAA AGCACTGAAA
    ACATACAATA TGCTTTGCTT TGGAATTTAT CGATTAAGGG ACGCTCATCT AAGTACTCCC
    AGCCAATGCA CTAAACGTTA TGTTATCACA AACCCACCAT ATGAGTTTGA GCTTGTGCCG
    ACAGACTTGA TCTTCTGTTT GATGCAATTC GACCACAATG CTGGCCAGTC CCGGGCCAGT
    CTTTCTCATT CCTCCCATTC CTCCTATTCT TCCAGCAAGA AGAGCTCATC AGTTCACTCC
    ATCCCATCAA CAGCAAACCG ACCGAACCGC ACCAAGACCA GGGATTCCCG GGAAAAACAG
    AAAAAAGAAA TGGTTTACAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025007208.1
    CDS368..3931
    Translation

    Target ORF information:

    RefSeq Version XM_025151440.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X41, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025151440.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTT
    GAAGATGAGC AGCCGTCAAC ACTATCACCC AAAAAAAAGC AACGAAATGG AGGCATGCGA
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAT
    GAACAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACGGG AATGTTTCAT
    TGGTGTCCAG CCAAGGATAT TGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACCCT GCATAACTTC
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGCTTTA CACCCCCTGG TATGGATAGG
    TCATCTCCAG ACAACAGTCC TGTCCACGGC CTGTTACGTC AACCCTCCAT TACAACAGGA
    GCCAACATCC CTATTATAAC AGAGCTAGCT AAGCCTGGCA AACTTCTGCC TTTGGTTTCA
    ATCAGTCAGG AAAAAAACAG TGGAACCCAC ATTCTAATGA TAACTGAACT GGTAAATGAT
    TCAAATGTTC AGTTCTTGGA CCAAGATGAT GATGATGATC CAGATACAGA ACTGTACCTC
    ACGCAGCCCT TTGCATGTGG AACCGCATTT GCTGTCAGCG TGCTGGACTC TCTCATGAGC
    GCAACATACT TCAATGACAA TATCCTCACA CTGATACGGA CATTGGTAAC AGGAGGAGCC
    ACACCAGAGC TGGAAGCACT GATTGCCGAG GAAAATGCTT TGAGAGGAGG TTACAGCACG
    CCCCAGACAC TTGCAAACAG GGACAGGTGT CGAGTTGCTC AGTTGGCTTT GTATGATGGG
    CCATTTGCAG ACCTAGGGGA TGGAGGCTGC TATGGAGATC TATTTTGTAA AGCACTGAAA
    ACATACAATA TGCTTTGCTT TGGAATTTAT CGATTAAGGG ACGCTCATCT AAGTACTCCC
    AGCCAATGCA CTAAACGTTA TGTTATCACA AACCCACCAT ATGAGTTTGA GCTTGTGCCG
    ACAGACTTGA TCTTCTGTTT GATGCAATTC GACCACAATG CTGGCCAGTC CCGGGCCAGT
    CTTTCTCATT CCTCCCATTC CTCCTATTCT TCCAGCAAGA AGAGCTCATC AGTTCACTCC
    ATCCCATCAA CAGCAAACCG ACCGAACCGC ACCAAGACCA GGGATTCCCG GGAAAAACAG
    AAAAAAGAAA TGGTTTACAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa151415
    Clone ID Related Accession (Same CDS sequence) XM_025151442.1
    Accession Version XM_025151442.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3480bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X44
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGTAAAT GATTCAAATG TTCAGTTCTT GGACCAAGAT
    GATGATGATG ATCCAGATAC AGAACTGTAC CTCACGCAGC CCTTTGCATG TGGAACCGCA
    TTTGCTGTCA GCGTGCTGGA CTCTCTCATG AGCGCAACAT ACTTCAATGA CAATATCCTC
    ACACTGATAC GGACATTGGT AACAGGAGGA GCCACACCAG AGCTGGAAGC ACTGATTGCC
    GAGGAAAATG CTTTGAGAGG AGGTTACAGC ACGCCCCAGA CACTTGCAAA CAGGGACAGG
    TGTCGAGTTG CTCAGTTGGC TTTGTATGAT GGGCCATTTG CAGACCTAGG GGATGGAGGC
    TGCTATGGAG ATCTATTTTG TAAAGCACTG AAAACATACA ATATGCTTTG CTTTGGAATT
    TATCGATTAA GGGACGCTCA TCTAAGTACT CCCAGCCAAT GCACTAAACG TTATGTTATC
    ACAAACCCAC CATATGAGTT TGAGCTTGTG CCGACAGACT TGATCTTCTG TTTGATGCAA
    TTCGACCACA ATGCTGGCCA GTCCCGGGCC AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT
    TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC TCCATCCCAT CAACAGCAAA CCGACCGAAC
    CGCACCAAGA CCAGGGATTC CCGGGAAAAA CAGAAGTATG TGCAGGAAGA TAGACTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025007210.1
    CDS116..3595
    Translation

    Target ORF information:

    RefSeq Version XM_025151442.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X44, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025151442.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGTAAAT GATTCAAATG TTCAGTTCTT GGACCAAGAT
    GATGATGATG ATCCAGATAC AGAACTGTAC CTCACGCAGC CCTTTGCATG TGGAACCGCA
    TTTGCTGTCA GCGTGCTGGA CTCTCTCATG AGCGCAACAT ACTTCAATGA CAATATCCTC
    ACACTGATAC GGACATTGGT AACAGGAGGA GCCACACCAG AGCTGGAAGC ACTGATTGCC
    GAGGAAAATG CTTTGAGAGG AGGTTACAGC ACGCCCCAGA CACTTGCAAA CAGGGACAGG
    TGTCGAGTTG CTCAGTTGGC TTTGTATGAT GGGCCATTTG CAGACCTAGG GGATGGAGGC
    TGCTATGGAG ATCTATTTTG TAAAGCACTG AAAACATACA ATATGCTTTG CTTTGGAATT
    TATCGATTAA GGGACGCTCA TCTAAGTACT CCCAGCCAAT GCACTAAACG TTATGTTATC
    ACAAACCCAC CATATGAGTT TGAGCTTGTG CCGACAGACT TGATCTTCTG TTTGATGCAA
    TTCGACCACA ATGCTGGCCA GTCCCGGGCC AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT
    TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC TCCATCCCAT CAACAGCAAA CCGACCGAAC
    CGCACCAAGA CCAGGGATTC CCGGGAAAAA CAGAAGTATG TGCAGGAAGA TAGACTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa151416
    Clone ID Related Accession (Same CDS sequence) XM_025151443.1
    Accession Version XM_025151443.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3477bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X46
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGTAAAT GATTCAAATG TTCAGTTCTT GGACCAAGAT
    GATGATGATG ATCCAGATAC AGAACTGTAC CTCACGCAGC CCTTTGCATG TGGAACCGCA
    TTTGCTGTCA GCGTGCTGGA CTCTCTCATG AGCGCAACAT ACTTCAATGA CAATATCCTC
    ACACTGATAC GGACATTGGT AACAGGAGGA GCCACACCAG AGCTGGAAGC ACTGATTGCC
    GAGGAAAATG CTTTGAGAGG AGGTTACAGC ACGCCCCAGA CACTTGCAAA CAGGGACAGG
    TGTCGAGTTG CTCAGTTGGC TTTGTATGAT GGGCCATTTG CAGACCTAGG GGATGGAGGC
    TGCTATGGAG ATCTATTTTG TAAAGCACTG AAAACATACA ATATGCTTTG CTTTGGAATT
    TATCGATTAA GGGACGCTCA TCTAAGTACT CCCAGCCAAT GCACTAAACG TTATGTTATC
    ACAAACCCAC CATATGAGTT TGAGCTTGTG CCGACAGACT TGATCTTCTG TTTGATGCAA
    TTCGACCACA ATGCTGGCCA GTCCCGGGCC AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT
    TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC TCCATCCCAT CAACAGCAAA CCGACCGAAC
    CGCACCAAGA CCAGGGATTC CCGGGAAAAA CAGAAAAAAG AAATGGTTTA CAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025007211.1
    CDS367..3843
    Translation

    Target ORF information:

    RefSeq Version XM_025151443.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X46, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025151443.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGTAAAT GATTCAAATG TTCAGTTCTT GGACCAAGAT
    GATGATGATG ATCCAGATAC AGAACTGTAC CTCACGCAGC CCTTTGCATG TGGAACCGCA
    TTTGCTGTCA GCGTGCTGGA CTCTCTCATG AGCGCAACAT ACTTCAATGA CAATATCCTC
    ACACTGATAC GGACATTGGT AACAGGAGGA GCCACACCAG AGCTGGAAGC ACTGATTGCC
    GAGGAAAATG CTTTGAGAGG AGGTTACAGC ACGCCCCAGA CACTTGCAAA CAGGGACAGG
    TGTCGAGTTG CTCAGTTGGC TTTGTATGAT GGGCCATTTG CAGACCTAGG GGATGGAGGC
    TGCTATGGAG ATCTATTTTG TAAAGCACTG AAAACATACA ATATGCTTTG CTTTGGAATT
    TATCGATTAA GGGACGCTCA TCTAAGTACT CCCAGCCAAT GCACTAAACG TTATGTTATC
    ACAAACCCAC CATATGAGTT TGAGCTTGTG CCGACAGACT TGATCTTCTG TTTGATGCAA
    TTCGACCACA ATGCTGGCCA GTCCCGGGCC AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT
    TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC TCCATCCCAT CAACAGCAAA CCGACCGAAC
    CGCACCAAGA CCAGGGATTC CCGGGAAAAA CAGAAAAAAG AAATGGTTTA CAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37420
    Clone ID Related Accession (Same CDS sequence) XM_015288147.2 , XM_015288147.1
    Accession Version XM_015288147.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3909bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288147.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GCTAAGCCTG GCAAACTTCT GCCTTTGGTT
    TCAATCAGTC AGGAAAAAAA CAGTGGAACC CACATTCTAA TGATAACTGA ACTGGTAAAT
    GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGATAC AGAACTGTAC
    CTCACGCAGC CCTTTGCATG TGGAACCGCA TTTGCTGTCA GCGTGCTGGA CTCTCTCATG
    AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA
    GCCACACCAG AGCTGGAAGC ACTGATTGCC GAGGAAAATG CTTTGAGAGG AGGTTACAGC
    ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGTTGGC TTTGTATGAT
    GGGCCATTTG CAGACCTAGG GGATGGAGGC TGCTATGGAG ATCTATTTTG TAAAGCACTG
    AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGACGCTCA TCTAAGTACT
    CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CATATGAGTT TGAGCTTGTG
    CCGACAGACT TGATCTTCTG TTTGATGCAA TTCGACCACA ATGCTGGCCA GTCCCGGGCC
    AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC
    TCCATCCCAT CAACAGCAAA CCGACCGAAC CGCACCAAGA CCAGGGATTC CCGGGAAAAA
    CAGAATGCAA CAAGGATGAA TAGAATGGGC CAAGCAGAAA AGAAATGGTT TACAGATGAA
    CCGGATAATG CCTATCCCAG AAACATTCAA ATCAAGCCCA TGAGCACCCA CATGGCCAAT
    CAGATCAATC AATACAAATC GACAAGCAGC TTGATACCAC CAATCAGAGA AGTTGAAGAT
    GAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143633.1
    CDS370..4278
    Translation

    Target ORF information:

    RefSeq Version XM_015288147.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288147.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GCTAAGCCTG GCAAACTTCT GCCTTTGGTT
    TCAATCAGTC AGGAAAAAAA CAGTGGAACC CACATTCTAA TGATAACTGA ACTGGTAAAT
    GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGATAC AGAACTGTAC
    CTCACGCAGC CCTTTGCATG TGGAACCGCA TTTGCTGTCA GCGTGCTGGA CTCTCTCATG
    AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA
    GCCACACCAG AGCTGGAAGC ACTGATTGCC GAGGAAAATG CTTTGAGAGG AGGTTACAGC
    ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGTTGGC TTTGTATGAT
    GGGCCATTTG CAGACCTAGG GGATGGAGGC TGCTATGGAG ATCTATTTTG TAAAGCACTG
    AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGACGCTCA TCTAAGTACT
    CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CATATGAGTT TGAGCTTGTG
    CCGACAGACT TGATCTTCTG TTTGATGCAA TTCGACCACA ATGCTGGCCA GTCCCGGGCC
    AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC
    TCCATCCCAT CAACAGCAAA CCGACCGAAC CGCACCAAGA CCAGGGATTC CCGGGAAAAA
    CAGAATGCAA CAAGGATGAA TAGAATGGGC CAAGCAGAAA AGAAATGGTT TACAGATGAA
    CCGGATAATG CCTATCCCAG AAACATTCAA ATCAAGCCCA TGAGCACCCA CATGGCCAAT
    CAGATCAATC AATACAAATC GACAAGCAGC TTGATACCAC CAATCAGAGA AGTTGAAGAT
    GAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37420
    Clone ID Related Accession (Same CDS sequence) XM_015288147.2 , XM_015288147.1
    Accession Version XM_015288147.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3909bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GCTAAGCCTG GCAAACTTCT GCCTTTGGTT
    TCAATCAGTC AGGAAAAAAA CAGTGGAACC CACATTCTAA TGATAACTGA ACTGGTAAAT
    GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGATAC AGAACTGTAC
    CTCACGCAGC CCTTTGCATG TGGAACCGCA TTTGCTGTCA GCGTGCTGGA CTCTCTCATG
    AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA
    GCCACACCAG AGCTGGAAGC ACTGATTGCC GAGGAAAATG CTTTGAGAGG AGGTTACAGC
    ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGTTGGC TTTGTATGAT
    GGGCCATTTG CAGACCTAGG GGATGGAGGC TGCTATGGAG ATCTATTTTG TAAAGCACTG
    AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGACGCTCA TCTAAGTACT
    CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CATATGAGTT TGAGCTTGTG
    CCGACAGACT TGATCTTCTG TTTGATGCAA TTCGACCACA ATGCTGGCCA GTCCCGGGCC
    AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC
    TCCATCCCAT CAACAGCAAA CCGACCGAAC CGCACCAAGA CCAGGGATTC CCGGGAAAAA
    CAGAATGCAA CAAGGATGAA TAGAATGGGC CAAGCAGAAA AGAAATGGTT TACAGATGAA
    CCGGATAATG CCTATCCCAG AAACATTCAA ATCAAGCCCA TGAGCACCCA CATGGCCAAT
    CAGATCAATC AATACAAATC GACAAGCAGC TTGATACCAC CAATCAGAGA AGTTGAAGAT
    GAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143633.1
    CDS374..4282
    Translation

    Target ORF information:

    RefSeq Version XM_015288147.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288147.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GCTAAGCCTG GCAAACTTCT GCCTTTGGTT
    TCAATCAGTC AGGAAAAAAA CAGTGGAACC CACATTCTAA TGATAACTGA ACTGGTAAAT
    GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGATAC AGAACTGTAC
    CTCACGCAGC CCTTTGCATG TGGAACCGCA TTTGCTGTCA GCGTGCTGGA CTCTCTCATG
    AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA
    GCCACACCAG AGCTGGAAGC ACTGATTGCC GAGGAAAATG CTTTGAGAGG AGGTTACAGC
    ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGTTGGC TTTGTATGAT
    GGGCCATTTG CAGACCTAGG GGATGGAGGC TGCTATGGAG ATCTATTTTG TAAAGCACTG
    AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGACGCTCA TCTAAGTACT
    CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CATATGAGTT TGAGCTTGTG
    CCGACAGACT TGATCTTCTG TTTGATGCAA TTCGACCACA ATGCTGGCCA GTCCCGGGCC
    AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC
    TCCATCCCAT CAACAGCAAA CCGACCGAAC CGCACCAAGA CCAGGGATTC CCGGGAAAAA
    CAGAATGCAA CAAGGATGAA TAGAATGGGC CAAGCAGAAA AGAAATGGTT TACAGATGAA
    CCGGATAATG CCTATCCCAG AAACATTCAA ATCAAGCCCA TGAGCACCCA CATGGCCAAT
    CAGATCAATC AATACAAATC GACAAGCAGC TTGATACCAC CAATCAGAGA AGTTGAAGAT
    GAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37421
    Clone ID Related Accession (Same CDS sequence) XM_015288148.1 , XM_015288148.2
    Accession Version XM_015288148.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GCTAAGCCTG GCAAACTTCT GCCTTTGGTT
    TCAATCAGTC AGGAAAAAAA CAGTGGAACC CACATTCTAA TGATAACTGA ACTGGTAAAT
    GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGATAC AGAACTGTAC
    CTCACGCAGC CCTTTGCATG TGGAACCGCA TTTGCTGTCA GCGTGCTGGA CTCTCTCATG
    AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA
    GCCACACCAG AGCTGGAAGC ACTGATTGCC GAGGAAAATG CTTTGAGAGG AGGTTACAGC
    ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGTTGGC TTTGTATGAT
    GGGCCATTTG CAGACCTAGG GGATGGAGGC TGCTATGGAG ATCTATTTTG TAAAGCACTG
    AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGACGCTCA TCTAAGTACT
    CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CATATGAGTT TGAGCTTGTG
    CCGACAGACT TGATCTTCTG TTTGATGCAA TTCGACCACA ATGCTGGCCA GTCCCGGGCC
    AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC
    TCCATCCCAT CAACAGCAAA CCGACCGAAC CGCACCAAGA CCAGGGATTC CCGGGAAAAA
    CAGAATGCAA CAAGGATGAA TAGAATGGGC CAAGAAAAGA AATGGTTTAC AGATGAACCG
    GATAATGCCT ATCCCAGAAA CATTCAAATC AAGCCCATGA GCACCCACAT GGCCAATCAG
    ATCAATCAAT ACAAATCGAC AAGCAGCTTG ATACCACCAA TCAGAGAAGT TGAAGATGAA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143634.1
    CDS374..4279
    Translation

    Target ORF information:

    RefSeq Version XM_015288148.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288148.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GCTAAGCCTG GCAAACTTCT GCCTTTGGTT
    TCAATCAGTC AGGAAAAAAA CAGTGGAACC CACATTCTAA TGATAACTGA ACTGGTAAAT
    GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGATAC AGAACTGTAC
    CTCACGCAGC CCTTTGCATG TGGAACCGCA TTTGCTGTCA GCGTGCTGGA CTCTCTCATG
    AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA
    GCCACACCAG AGCTGGAAGC ACTGATTGCC GAGGAAAATG CTTTGAGAGG AGGTTACAGC
    ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGTTGGC TTTGTATGAT
    GGGCCATTTG CAGACCTAGG GGATGGAGGC TGCTATGGAG ATCTATTTTG TAAAGCACTG
    AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGACGCTCA TCTAAGTACT
    CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CATATGAGTT TGAGCTTGTG
    CCGACAGACT TGATCTTCTG TTTGATGCAA TTCGACCACA ATGCTGGCCA GTCCCGGGCC
    AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC
    TCCATCCCAT CAACAGCAAA CCGACCGAAC CGCACCAAGA CCAGGGATTC CCGGGAAAAA
    CAGAATGCAA CAAGGATGAA TAGAATGGGC CAAGAAAAGA AATGGTTTAC AGATGAACCG
    GATAATGCCT ATCCCAGAAA CATTCAAATC AAGCCCATGA GCACCCACAT GGCCAATCAG
    ATCAATCAAT ACAAATCGAC AAGCAGCTTG ATACCACCAA TCAGAGAAGT TGAAGATGAA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37421
    Clone ID Related Accession (Same CDS sequence) XM_015288148.1 , XM_015288148.2
    Accession Version XM_015288148.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288148.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GCTAAGCCTG GCAAACTTCT GCCTTTGGTT
    TCAATCAGTC AGGAAAAAAA CAGTGGAACC CACATTCTAA TGATAACTGA ACTGGTAAAT
    GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGATAC AGAACTGTAC
    CTCACGCAGC CCTTTGCATG TGGAACCGCA TTTGCTGTCA GCGTGCTGGA CTCTCTCATG
    AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA
    GCCACACCAG AGCTGGAAGC ACTGATTGCC GAGGAAAATG CTTTGAGAGG AGGTTACAGC
    ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGTTGGC TTTGTATGAT
    GGGCCATTTG CAGACCTAGG GGATGGAGGC TGCTATGGAG ATCTATTTTG TAAAGCACTG
    AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGACGCTCA TCTAAGTACT
    CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CATATGAGTT TGAGCTTGTG
    CCGACAGACT TGATCTTCTG TTTGATGCAA TTCGACCACA ATGCTGGCCA GTCCCGGGCC
    AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC
    TCCATCCCAT CAACAGCAAA CCGACCGAAC CGCACCAAGA CCAGGGATTC CCGGGAAAAA
    CAGAATGCAA CAAGGATGAA TAGAATGGGC CAAGAAAAGA AATGGTTTAC AGATGAACCG
    GATAATGCCT ATCCCAGAAA CATTCAAATC AAGCCCATGA GCACCCACAT GGCCAATCAG
    ATCAATCAAT ACAAATCGAC AAGCAGCTTG ATACCACCAA TCAGAGAAGT TGAAGATGAA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143634.1
    CDS370..4275
    Translation

    Target ORF information:

    RefSeq Version XM_015288148.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288148.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GCTAAGCCTG GCAAACTTCT GCCTTTGGTT
    TCAATCAGTC AGGAAAAAAA CAGTGGAACC CACATTCTAA TGATAACTGA ACTGGTAAAT
    GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGATAC AGAACTGTAC
    CTCACGCAGC CCTTTGCATG TGGAACCGCA TTTGCTGTCA GCGTGCTGGA CTCTCTCATG
    AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA
    GCCACACCAG AGCTGGAAGC ACTGATTGCC GAGGAAAATG CTTTGAGAGG AGGTTACAGC
    ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGTTGGC TTTGTATGAT
    GGGCCATTTG CAGACCTAGG GGATGGAGGC TGCTATGGAG ATCTATTTTG TAAAGCACTG
    AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGACGCTCA TCTAAGTACT
    CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CATATGAGTT TGAGCTTGTG
    CCGACAGACT TGATCTTCTG TTTGATGCAA TTCGACCACA ATGCTGGCCA GTCCCGGGCC
    AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC
    TCCATCCCAT CAACAGCAAA CCGACCGAAC CGCACCAAGA CCAGGGATTC CCGGGAAAAA
    CAGAATGCAA CAAGGATGAA TAGAATGGGC CAAGAAAAGA AATGGTTTAC AGATGAACCG
    GATAATGCCT ATCCCAGAAA CATTCAAATC AAGCCCATGA GCACCCACAT GGCCAATCAG
    ATCAATCAAT ACAAATCGAC AAGCAGCTTG ATACCACCAA TCAGAGAAGT TGAAGATGAA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37422
    Clone ID Related Accession (Same CDS sequence) XM_015288149.1 , XM_015288149.2
    Accession Version XM_015288149.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3900bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCCC
    AAGATGTCCA TCTACAAGAG AATGAAACTG GCATGTTGTT TTGATTGCGG ACGCTCTGAG
    CGTGACTGCT CTTGCATGTC AGGCAGTGTA CATAGTAACA TGGACACCCT TGAGAGAGCC
    TTCCCACTTT CTTCTGTCTC TGTTAATGAT TGCTCCACCA GTTTACGTGC CTTTGAAGAT
    GAGCAGCCGT CAACACTATC ACCCAAAAAA AAGCAACGAA ATGGAGGCAT GCGAAATTCA
    CCCAACTCCT CACCCAAACT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAATGAACAG
    ATTGACAATA TGGATGCCAA TGTGAAAAAA TATGACTCTA CGGGAATGTT TCATTGGTGT
    CCAGCCAAGG ATATTGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA
    AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA
    AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG
    TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CCCTGCATAA CTTCCCCAAG
    GTTTCAATCT TGCCTGGTAC ACCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC
    CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG
    CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC
    ATTGGGGTCT TGCAGGCTAA TTCCCAAGGC TTTACACCCC CTGGTATGGA TAGGTCATCT
    CCAGACAACA GTCCTGTCCA CGGCCTGTTA CGTCAACCCT CCATTACAAC AGGAGCCAAC
    ATCCCTATTA TAACAGAGCT AGCTAAGCCT GGCAAACTTC TGCCTTTGGT TTCAATCAGT
    CAGGAAAAAA ACAGTGGAAC CCACATTCTA ATGATAACTG AACTGGTAAA TGATTCAAAT
    GTTCAGTTCT TGGACCAAGA TGATGATGAT GATCCAGATA CAGAACTGTA CCTCACGCAG
    CCCTTTGCAT GTGGAACCGC ATTTGCTGTC AGCGTGCTGG ACTCTCTCAT GAGCGCAACA
    TACTTCAATG ACAATATCCT CACACTGATA CGGACATTGG TAACAGGAGG AGCCACACCA
    GAGCTGGAAG CACTGATTGC CGAGGAAAAT GCTTTGAGAG GAGGTTACAG CACGCCCCAG
    ACACTTGCAA ACAGGGACAG GTGTCGAGTT GCTCAGTTGG CTTTGTATGA TGGGCCATTT
    GCAGACCTAG GGGATGGAGG CTGCTATGGA GATCTATTTT GTAAAGCACT GAAAACATAC
    AATATGCTTT GCTTTGGAAT TTATCGATTA AGGGACGCTC ATCTAAGTAC TCCCAGCCAA
    TGCACTAAAC GTTATGTTAT CACAAACCCA CCATATGAGT TTGAGCTTGT GCCGACAGAC
    TTGATCTTCT GTTTGATGCA ATTCGACCAC AATGCTGGCC AGTCCCGGGC CAGTCTTTCT
    CATTCCTCCC ATTCCTCCTA TTCTTCCAGC AAGAAGAGCT CATCAGTTCA CTCCATCCCA
    TCAACAGCAA ACCGACCGAA CCGCACCAAG ACCAGGGATT CCCGGGAAAA ACAGAATGCA
    ACAAGGATGA ATAGAATGGG CCAAGCAGAA AAGAAATGGT TTACAGATGA ACCGGATAAT
    GCCTATCCCA GAAACATTCA AATCAAGCCC ATGAGCACCC ACATGGCCAA TCAGATCAAT
    CAATACAAAT CGACAAGCAG CTTGATACCA CCAATCAGAG AAGTTGAAGA TGAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143635.1
    CDS374..4273
    Translation

    Target ORF information:

    RefSeq Version XM_015288149.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288149.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCCC
    AAGATGTCCA TCTACAAGAG AATGAAACTG GCATGTTGTT TTGATTGCGG ACGCTCTGAG
    CGTGACTGCT CTTGCATGTC AGGCAGTGTA CATAGTAACA TGGACACCCT TGAGAGAGCC
    TTCCCACTTT CTTCTGTCTC TGTTAATGAT TGCTCCACCA GTTTACGTGC CTTTGAAGAT
    GAGCAGCCGT CAACACTATC ACCCAAAAAA AAGCAACGAA ATGGAGGCAT GCGAAATTCA
    CCCAACTCCT CACCCAAACT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAATGAACAG
    ATTGACAATA TGGATGCCAA TGTGAAAAAA TATGACTCTA CGGGAATGTT TCATTGGTGT
    CCAGCCAAGG ATATTGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA
    AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA
    AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG
    TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CCCTGCATAA CTTCCCCAAG
    GTTTCAATCT TGCCTGGTAC ACCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC
    CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG
    CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC
    ATTGGGGTCT TGCAGGCTAA TTCCCAAGGC TTTACACCCC CTGGTATGGA TAGGTCATCT
    CCAGACAACA GTCCTGTCCA CGGCCTGTTA CGTCAACCCT CCATTACAAC AGGAGCCAAC
    ATCCCTATTA TAACAGAGCT AGCTAAGCCT GGCAAACTTC TGCCTTTGGT TTCAATCAGT
    CAGGAAAAAA ACAGTGGAAC CCACATTCTA ATGATAACTG AACTGGTAAA TGATTCAAAT
    GTTCAGTTCT TGGACCAAGA TGATGATGAT GATCCAGATA CAGAACTGTA CCTCACGCAG
    CCCTTTGCAT GTGGAACCGC ATTTGCTGTC AGCGTGCTGG ACTCTCTCAT GAGCGCAACA
    TACTTCAATG ACAATATCCT CACACTGATA CGGACATTGG TAACAGGAGG AGCCACACCA
    GAGCTGGAAG CACTGATTGC CGAGGAAAAT GCTTTGAGAG GAGGTTACAG CACGCCCCAG
    ACACTTGCAA ACAGGGACAG GTGTCGAGTT GCTCAGTTGG CTTTGTATGA TGGGCCATTT
    GCAGACCTAG GGGATGGAGG CTGCTATGGA GATCTATTTT GTAAAGCACT GAAAACATAC
    AATATGCTTT GCTTTGGAAT TTATCGATTA AGGGACGCTC ATCTAAGTAC TCCCAGCCAA
    TGCACTAAAC GTTATGTTAT CACAAACCCA CCATATGAGT TTGAGCTTGT GCCGACAGAC
    TTGATCTTCT GTTTGATGCA ATTCGACCAC AATGCTGGCC AGTCCCGGGC CAGTCTTTCT
    CATTCCTCCC ATTCCTCCTA TTCTTCCAGC AAGAAGAGCT CATCAGTTCA CTCCATCCCA
    TCAACAGCAA ACCGACCGAA CCGCACCAAG ACCAGGGATT CCCGGGAAAA ACAGAATGCA
    ACAAGGATGA ATAGAATGGG CCAAGCAGAA AAGAAATGGT TTACAGATGA ACCGGATAAT
    GCCTATCCCA GAAACATTCA AATCAAGCCC ATGAGCACCC ACATGGCCAA TCAGATCAAT
    CAATACAAAT CGACAAGCAG CTTGATACCA CCAATCAGAG AAGTTGAAGA TGAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37422
    Clone ID Related Accession (Same CDS sequence) XM_015288149.1 , XM_015288149.2
    Accession Version XM_015288149.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3900bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288149.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCCC
    AAGATGTCCA TCTACAAGAG AATGAAACTG GCATGTTGTT TTGATTGCGG ACGCTCTGAG
    CGTGACTGCT CTTGCATGTC AGGCAGTGTA CATAGTAACA TGGACACCCT TGAGAGAGCC
    TTCCCACTTT CTTCTGTCTC TGTTAATGAT TGCTCCACCA GTTTACGTGC CTTTGAAGAT
    GAGCAGCCGT CAACACTATC ACCCAAAAAA AAGCAACGAA ATGGAGGCAT GCGAAATTCA
    CCCAACTCCT CACCCAAACT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAATGAACAG
    ATTGACAATA TGGATGCCAA TGTGAAAAAA TATGACTCTA CGGGAATGTT TCATTGGTGT
    CCAGCCAAGG ATATTGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA
    AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA
    AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG
    TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CCCTGCATAA CTTCCCCAAG
    GTTTCAATCT TGCCTGGTAC ACCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC
    CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG
    CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC
    ATTGGGGTCT TGCAGGCTAA TTCCCAAGGC TTTACACCCC CTGGTATGGA TAGGTCATCT
    CCAGACAACA GTCCTGTCCA CGGCCTGTTA CGTCAACCCT CCATTACAAC AGGAGCCAAC
    ATCCCTATTA TAACAGAGCT AGCTAAGCCT GGCAAACTTC TGCCTTTGGT TTCAATCAGT
    CAGGAAAAAA ACAGTGGAAC CCACATTCTA ATGATAACTG AACTGGTAAA TGATTCAAAT
    GTTCAGTTCT TGGACCAAGA TGATGATGAT GATCCAGATA CAGAACTGTA CCTCACGCAG
    CCCTTTGCAT GTGGAACCGC ATTTGCTGTC AGCGTGCTGG ACTCTCTCAT GAGCGCAACA
    TACTTCAATG ACAATATCCT CACACTGATA CGGACATTGG TAACAGGAGG AGCCACACCA
    GAGCTGGAAG CACTGATTGC CGAGGAAAAT GCTTTGAGAG GAGGTTACAG CACGCCCCAG
    ACACTTGCAA ACAGGGACAG GTGTCGAGTT GCTCAGTTGG CTTTGTATGA TGGGCCATTT
    GCAGACCTAG GGGATGGAGG CTGCTATGGA GATCTATTTT GTAAAGCACT GAAAACATAC
    AATATGCTTT GCTTTGGAAT TTATCGATTA AGGGACGCTC ATCTAAGTAC TCCCAGCCAA
    TGCACTAAAC GTTATGTTAT CACAAACCCA CCATATGAGT TTGAGCTTGT GCCGACAGAC
    TTGATCTTCT GTTTGATGCA ATTCGACCAC AATGCTGGCC AGTCCCGGGC CAGTCTTTCT
    CATTCCTCCC ATTCCTCCTA TTCTTCCAGC AAGAAGAGCT CATCAGTTCA CTCCATCCCA
    TCAACAGCAA ACCGACCGAA CCGCACCAAG ACCAGGGATT CCCGGGAAAA ACAGAATGCA
    ACAAGGATGA ATAGAATGGG CCAAGCAGAA AAGAAATGGT TTACAGATGA ACCGGATAAT
    GCCTATCCCA GAAACATTCA AATCAAGCCC ATGAGCACCC ACATGGCCAA TCAGATCAAT
    CAATACAAAT CGACAAGCAG CTTGATACCA CCAATCAGAG AAGTTGAAGA TGAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143635.1
    CDS370..4269
    Translation

    Target ORF information:

    RefSeq Version XM_015288149.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288149.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCCC
    AAGATGTCCA TCTACAAGAG AATGAAACTG GCATGTTGTT TTGATTGCGG ACGCTCTGAG
    CGTGACTGCT CTTGCATGTC AGGCAGTGTA CATAGTAACA TGGACACCCT TGAGAGAGCC
    TTCCCACTTT CTTCTGTCTC TGTTAATGAT TGCTCCACCA GTTTACGTGC CTTTGAAGAT
    GAGCAGCCGT CAACACTATC ACCCAAAAAA AAGCAACGAA ATGGAGGCAT GCGAAATTCA
    CCCAACTCCT CACCCAAACT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAATGAACAG
    ATTGACAATA TGGATGCCAA TGTGAAAAAA TATGACTCTA CGGGAATGTT TCATTGGTGT
    CCAGCCAAGG ATATTGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA
    AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA
    AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG
    TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CCCTGCATAA CTTCCCCAAG
    GTTTCAATCT TGCCTGGTAC ACCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC
    CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG
    CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC
    ATTGGGGTCT TGCAGGCTAA TTCCCAAGGC TTTACACCCC CTGGTATGGA TAGGTCATCT
    CCAGACAACA GTCCTGTCCA CGGCCTGTTA CGTCAACCCT CCATTACAAC AGGAGCCAAC
    ATCCCTATTA TAACAGAGCT AGCTAAGCCT GGCAAACTTC TGCCTTTGGT TTCAATCAGT
    CAGGAAAAAA ACAGTGGAAC CCACATTCTA ATGATAACTG AACTGGTAAA TGATTCAAAT
    GTTCAGTTCT TGGACCAAGA TGATGATGAT GATCCAGATA CAGAACTGTA CCTCACGCAG
    CCCTTTGCAT GTGGAACCGC ATTTGCTGTC AGCGTGCTGG ACTCTCTCAT GAGCGCAACA
    TACTTCAATG ACAATATCCT CACACTGATA CGGACATTGG TAACAGGAGG AGCCACACCA
    GAGCTGGAAG CACTGATTGC CGAGGAAAAT GCTTTGAGAG GAGGTTACAG CACGCCCCAG
    ACACTTGCAA ACAGGGACAG GTGTCGAGTT GCTCAGTTGG CTTTGTATGA TGGGCCATTT
    GCAGACCTAG GGGATGGAGG CTGCTATGGA GATCTATTTT GTAAAGCACT GAAAACATAC
    AATATGCTTT GCTTTGGAAT TTATCGATTA AGGGACGCTC ATCTAAGTAC TCCCAGCCAA
    TGCACTAAAC GTTATGTTAT CACAAACCCA CCATATGAGT TTGAGCTTGT GCCGACAGAC
    TTGATCTTCT GTTTGATGCA ATTCGACCAC AATGCTGGCC AGTCCCGGGC CAGTCTTTCT
    CATTCCTCCC ATTCCTCCTA TTCTTCCAGC AAGAAGAGCT CATCAGTTCA CTCCATCCCA
    TCAACAGCAA ACCGACCGAA CCGCACCAAG ACCAGGGATT CCCGGGAAAA ACAGAATGCA
    ACAAGGATGA ATAGAATGGG CCAAGCAGAA AAGAAATGGT TTACAGATGA ACCGGATAAT
    GCCTATCCCA GAAACATTCA AATCAAGCCC ATGAGCACCC ACATGGCCAA TCAGATCAAT
    CAATACAAAT CGACAAGCAG CTTGATACCA CCAATCAGAG AAGTTGAAGA TGAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37423
    Clone ID Related Accession (Same CDS sequence) XM_015288150.1 , XM_015288150.2
    Accession Version XM_015288150.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3897bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTCCAAG
    ATGTCCATCT ACAAGAGAAT GAAACTGGCA TGTTGTTTTG ATTGCGGACG CTCTGAGCGT
    GACTGCTCTT GCATGTCAGG CAGTGTACAT AGTAACATGG ACACCCTTGA GAGAGCCTTC
    CCACTTTCTT CTGTCTCTGT TAATGATTGC TCCACCAGTT TACGTGCCTT TGAAGATGAG
    CAGCCGTCAA CACTATCACC CAAAAAAAAG CAACGAAATG GAGGCATGCG AAATTCACCC
    AACTCCTCAC CCAAACTGAT GAGGCATGAC CCCTTGTTAA TTCCTGGCAA TGAACAGATT
    GACAATATGG ATGCCAATGT GAAAAAATAT GACTCTACGG GAATGTTTCA TTGGTGTCCA
    GCCAAGGATA TTGAAAAGGT CATTTTGACT CGAAGTGAAG CAGCGATGAC AGTTTTAAGT
    GGGCATGTAG TCGTTTGCAT ATTTGGGGAT GTTAAATCTG CTTTGATTGG ATTAAGAAAT
    TTAGTGATGC CATTACGAGC CAGCAACTTT CACTACCATG AACTCAAGCA CATTGTGTTT
    GTGGGTTCAC TTGAATACCT GAGAAGGGAA TGGGAGACCC TGCATAACTT CCCCAAGGTT
    TCAATCTTGC CTGGTACACC ATTAAGTCGG GCTGATTTAA GGGCTGTCAA CATCAACCTC
    TGCGACATGT GCGTTATCCT GTCAGCCAAT CAGAATAATA TTGATGATGC TTCGCTGCAG
    GACAAGGAAT GCATCTTGGC GTCACTCAAC ATCAAATCTA TGCAGTTTGA TGACAGCATT
    GGGGTCTTGC AGGCTAATTC CCAAGGCTTT ACACCCCCTG GTATGGATAG GTCATCTCCA
    GACAACAGTC CTGTCCACGG CCTGTTACGT CAACCCTCCA TTACAACAGG AGCCAACATC
    CCTATTATAA CAGAGCTAGC TAAGCCTGGC AAACTTCTGC CTTTGGTTTC AATCAGTCAG
    GAAAAAAACA GTGGAACCCA CATTCTAATG ATAACTGAAC TGGTAAATGA TTCAAATGTT
    CAGTTCTTGG ACCAAGATGA TGATGATGAT CCAGATACAG AACTGTACCT CACGCAGCCC
    TTTGCATGTG GAACCGCATT TGCTGTCAGC GTGCTGGACT CTCTCATGAG CGCAACATAC
    TTCAATGACA ATATCCTCAC ACTGATACGG ACATTGGTAA CAGGAGGAGC CACACCAGAG
    CTGGAAGCAC TGATTGCCGA GGAAAATGCT TTGAGAGGAG GTTACAGCAC GCCCCAGACA
    CTTGCAAACA GGGACAGGTG TCGAGTTGCT CAGTTGGCTT TGTATGATGG GCCATTTGCA
    GACCTAGGGG ATGGAGGCTG CTATGGAGAT CTATTTTGTA AAGCACTGAA AACATACAAT
    ATGCTTTGCT TTGGAATTTA TCGATTAAGG GACGCTCATC TAAGTACTCC CAGCCAATGC
    ACTAAACGTT ATGTTATCAC AAACCCACCA TATGAGTTTG AGCTTGTGCC GACAGACTTG
    ATCTTCTGTT TGATGCAATT CGACCACAAT GCTGGCCAGT CCCGGGCCAG TCTTTCTCAT
    TCCTCCCATT CCTCCTATTC TTCCAGCAAG AAGAGCTCAT CAGTTCACTC CATCCCATCA
    ACAGCAAACC GACCGAACCG CACCAAGACC AGGGATTCCC GGGAAAAACA GAATGCAACA
    AGGATGAATA GAATGGGCCA AGCAGAAAAG AAATGGTTTA CAGATGAACC GGATAATGCC
    TATCCCAGAA ACATTCAAAT CAAGCCCATG AGCACCCACA TGGCCAATCA GATCAATCAA
    TACAAATCGA CAAGCAGCTT GATACCACCA ATCAGAGAAG TTGAAGATGA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143636.1
    CDS374..4270
    Translation

    Target ORF information:

    RefSeq Version XM_015288150.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288150.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTCCAAG
    ATGTCCATCT ACAAGAGAAT GAAACTGGCA TGTTGTTTTG ATTGCGGACG CTCTGAGCGT
    GACTGCTCTT GCATGTCAGG CAGTGTACAT AGTAACATGG ACACCCTTGA GAGAGCCTTC
    CCACTTTCTT CTGTCTCTGT TAATGATTGC TCCACCAGTT TACGTGCCTT TGAAGATGAG
    CAGCCGTCAA CACTATCACC CAAAAAAAAG CAACGAAATG GAGGCATGCG AAATTCACCC
    AACTCCTCAC CCAAACTGAT GAGGCATGAC CCCTTGTTAA TTCCTGGCAA TGAACAGATT
    GACAATATGG ATGCCAATGT GAAAAAATAT GACTCTACGG GAATGTTTCA TTGGTGTCCA
    GCCAAGGATA TTGAAAAGGT CATTTTGACT CGAAGTGAAG CAGCGATGAC AGTTTTAAGT
    GGGCATGTAG TCGTTTGCAT ATTTGGGGAT GTTAAATCTG CTTTGATTGG ATTAAGAAAT
    TTAGTGATGC CATTACGAGC CAGCAACTTT CACTACCATG AACTCAAGCA CATTGTGTTT
    GTGGGTTCAC TTGAATACCT GAGAAGGGAA TGGGAGACCC TGCATAACTT CCCCAAGGTT
    TCAATCTTGC CTGGTACACC ATTAAGTCGG GCTGATTTAA GGGCTGTCAA CATCAACCTC
    TGCGACATGT GCGTTATCCT GTCAGCCAAT CAGAATAATA TTGATGATGC TTCGCTGCAG
    GACAAGGAAT GCATCTTGGC GTCACTCAAC ATCAAATCTA TGCAGTTTGA TGACAGCATT
    GGGGTCTTGC AGGCTAATTC CCAAGGCTTT ACACCCCCTG GTATGGATAG GTCATCTCCA
    GACAACAGTC CTGTCCACGG CCTGTTACGT CAACCCTCCA TTACAACAGG AGCCAACATC
    CCTATTATAA CAGAGCTAGC TAAGCCTGGC AAACTTCTGC CTTTGGTTTC AATCAGTCAG
    GAAAAAAACA GTGGAACCCA CATTCTAATG ATAACTGAAC TGGTAAATGA TTCAAATGTT
    CAGTTCTTGG ACCAAGATGA TGATGATGAT CCAGATACAG AACTGTACCT CACGCAGCCC
    TTTGCATGTG GAACCGCATT TGCTGTCAGC GTGCTGGACT CTCTCATGAG CGCAACATAC
    TTCAATGACA ATATCCTCAC ACTGATACGG ACATTGGTAA CAGGAGGAGC CACACCAGAG
    CTGGAAGCAC TGATTGCCGA GGAAAATGCT TTGAGAGGAG GTTACAGCAC GCCCCAGACA
    CTTGCAAACA GGGACAGGTG TCGAGTTGCT CAGTTGGCTT TGTATGATGG GCCATTTGCA
    GACCTAGGGG ATGGAGGCTG CTATGGAGAT CTATTTTGTA AAGCACTGAA AACATACAAT
    ATGCTTTGCT TTGGAATTTA TCGATTAAGG GACGCTCATC TAAGTACTCC CAGCCAATGC
    ACTAAACGTT ATGTTATCAC AAACCCACCA TATGAGTTTG AGCTTGTGCC GACAGACTTG
    ATCTTCTGTT TGATGCAATT CGACCACAAT GCTGGCCAGT CCCGGGCCAG TCTTTCTCAT
    TCCTCCCATT CCTCCTATTC TTCCAGCAAG AAGAGCTCAT CAGTTCACTC CATCCCATCA
    ACAGCAAACC GACCGAACCG CACCAAGACC AGGGATTCCC GGGAAAAACA GAATGCAACA
    AGGATGAATA GAATGGGCCA AGCAGAAAAG AAATGGTTTA CAGATGAACC GGATAATGCC
    TATCCCAGAA ACATTCAAAT CAAGCCCATG AGCACCCACA TGGCCAATCA GATCAATCAA
    TACAAATCGA CAAGCAGCTT GATACCACCA ATCAGAGAAG TTGAAGATGA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37423
    Clone ID Related Accession (Same CDS sequence) XM_015288150.1 , XM_015288150.2
    Accession Version XM_015288150.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3897bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288150.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTCCAAG
    ATGTCCATCT ACAAGAGAAT GAAACTGGCA TGTTGTTTTG ATTGCGGACG CTCTGAGCGT
    GACTGCTCTT GCATGTCAGG CAGTGTACAT AGTAACATGG ACACCCTTGA GAGAGCCTTC
    CCACTTTCTT CTGTCTCTGT TAATGATTGC TCCACCAGTT TACGTGCCTT TGAAGATGAG
    CAGCCGTCAA CACTATCACC CAAAAAAAAG CAACGAAATG GAGGCATGCG AAATTCACCC
    AACTCCTCAC CCAAACTGAT GAGGCATGAC CCCTTGTTAA TTCCTGGCAA TGAACAGATT
    GACAATATGG ATGCCAATGT GAAAAAATAT GACTCTACGG GAATGTTTCA TTGGTGTCCA
    GCCAAGGATA TTGAAAAGGT CATTTTGACT CGAAGTGAAG CAGCGATGAC AGTTTTAAGT
    GGGCATGTAG TCGTTTGCAT ATTTGGGGAT GTTAAATCTG CTTTGATTGG ATTAAGAAAT
    TTAGTGATGC CATTACGAGC CAGCAACTTT CACTACCATG AACTCAAGCA CATTGTGTTT
    GTGGGTTCAC TTGAATACCT GAGAAGGGAA TGGGAGACCC TGCATAACTT CCCCAAGGTT
    TCAATCTTGC CTGGTACACC ATTAAGTCGG GCTGATTTAA GGGCTGTCAA CATCAACCTC
    TGCGACATGT GCGTTATCCT GTCAGCCAAT CAGAATAATA TTGATGATGC TTCGCTGCAG
    GACAAGGAAT GCATCTTGGC GTCACTCAAC ATCAAATCTA TGCAGTTTGA TGACAGCATT
    GGGGTCTTGC AGGCTAATTC CCAAGGCTTT ACACCCCCTG GTATGGATAG GTCATCTCCA
    GACAACAGTC CTGTCCACGG CCTGTTACGT CAACCCTCCA TTACAACAGG AGCCAACATC
    CCTATTATAA CAGAGCTAGC TAAGCCTGGC AAACTTCTGC CTTTGGTTTC AATCAGTCAG
    GAAAAAAACA GTGGAACCCA CATTCTAATG ATAACTGAAC TGGTAAATGA TTCAAATGTT
    CAGTTCTTGG ACCAAGATGA TGATGATGAT CCAGATACAG AACTGTACCT CACGCAGCCC
    TTTGCATGTG GAACCGCATT TGCTGTCAGC GTGCTGGACT CTCTCATGAG CGCAACATAC
    TTCAATGACA ATATCCTCAC ACTGATACGG ACATTGGTAA CAGGAGGAGC CACACCAGAG
    CTGGAAGCAC TGATTGCCGA GGAAAATGCT TTGAGAGGAG GTTACAGCAC GCCCCAGACA
    CTTGCAAACA GGGACAGGTG TCGAGTTGCT CAGTTGGCTT TGTATGATGG GCCATTTGCA
    GACCTAGGGG ATGGAGGCTG CTATGGAGAT CTATTTTGTA AAGCACTGAA AACATACAAT
    ATGCTTTGCT TTGGAATTTA TCGATTAAGG GACGCTCATC TAAGTACTCC CAGCCAATGC
    ACTAAACGTT ATGTTATCAC AAACCCACCA TATGAGTTTG AGCTTGTGCC GACAGACTTG
    ATCTTCTGTT TGATGCAATT CGACCACAAT GCTGGCCAGT CCCGGGCCAG TCTTTCTCAT
    TCCTCCCATT CCTCCTATTC TTCCAGCAAG AAGAGCTCAT CAGTTCACTC CATCCCATCA
    ACAGCAAACC GACCGAACCG CACCAAGACC AGGGATTCCC GGGAAAAACA GAATGCAACA
    AGGATGAATA GAATGGGCCA AGCAGAAAAG AAATGGTTTA CAGATGAACC GGATAATGCC
    TATCCCAGAA ACATTCAAAT CAAGCCCATG AGCACCCACA TGGCCAATCA GATCAATCAA
    TACAAATCGA CAAGCAGCTT GATACCACCA ATCAGAGAAG TTGAAGATGA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143636.1
    CDS369..4265
    Translation

    Target ORF information:

    RefSeq Version XM_015288150.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288150.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTCCAAG
    ATGTCCATCT ACAAGAGAAT GAAACTGGCA TGTTGTTTTG ATTGCGGACG CTCTGAGCGT
    GACTGCTCTT GCATGTCAGG CAGTGTACAT AGTAACATGG ACACCCTTGA GAGAGCCTTC
    CCACTTTCTT CTGTCTCTGT TAATGATTGC TCCACCAGTT TACGTGCCTT TGAAGATGAG
    CAGCCGTCAA CACTATCACC CAAAAAAAAG CAACGAAATG GAGGCATGCG AAATTCACCC
    AACTCCTCAC CCAAACTGAT GAGGCATGAC CCCTTGTTAA TTCCTGGCAA TGAACAGATT
    GACAATATGG ATGCCAATGT GAAAAAATAT GACTCTACGG GAATGTTTCA TTGGTGTCCA
    GCCAAGGATA TTGAAAAGGT CATTTTGACT CGAAGTGAAG CAGCGATGAC AGTTTTAAGT
    GGGCATGTAG TCGTTTGCAT ATTTGGGGAT GTTAAATCTG CTTTGATTGG ATTAAGAAAT
    TTAGTGATGC CATTACGAGC CAGCAACTTT CACTACCATG AACTCAAGCA CATTGTGTTT
    GTGGGTTCAC TTGAATACCT GAGAAGGGAA TGGGAGACCC TGCATAACTT CCCCAAGGTT
    TCAATCTTGC CTGGTACACC ATTAAGTCGG GCTGATTTAA GGGCTGTCAA CATCAACCTC
    TGCGACATGT GCGTTATCCT GTCAGCCAAT CAGAATAATA TTGATGATGC TTCGCTGCAG
    GACAAGGAAT GCATCTTGGC GTCACTCAAC ATCAAATCTA TGCAGTTTGA TGACAGCATT
    GGGGTCTTGC AGGCTAATTC CCAAGGCTTT ACACCCCCTG GTATGGATAG GTCATCTCCA
    GACAACAGTC CTGTCCACGG CCTGTTACGT CAACCCTCCA TTACAACAGG AGCCAACATC
    CCTATTATAA CAGAGCTAGC TAAGCCTGGC AAACTTCTGC CTTTGGTTTC AATCAGTCAG
    GAAAAAAACA GTGGAACCCA CATTCTAATG ATAACTGAAC TGGTAAATGA TTCAAATGTT
    CAGTTCTTGG ACCAAGATGA TGATGATGAT CCAGATACAG AACTGTACCT CACGCAGCCC
    TTTGCATGTG GAACCGCATT TGCTGTCAGC GTGCTGGACT CTCTCATGAG CGCAACATAC
    TTCAATGACA ATATCCTCAC ACTGATACGG ACATTGGTAA CAGGAGGAGC CACACCAGAG
    CTGGAAGCAC TGATTGCCGA GGAAAATGCT TTGAGAGGAG GTTACAGCAC GCCCCAGACA
    CTTGCAAACA GGGACAGGTG TCGAGTTGCT CAGTTGGCTT TGTATGATGG GCCATTTGCA
    GACCTAGGGG ATGGAGGCTG CTATGGAGAT CTATTTTGTA AAGCACTGAA AACATACAAT
    ATGCTTTGCT TTGGAATTTA TCGATTAAGG GACGCTCATC TAAGTACTCC CAGCCAATGC
    ACTAAACGTT ATGTTATCAC AAACCCACCA TATGAGTTTG AGCTTGTGCC GACAGACTTG
    ATCTTCTGTT TGATGCAATT CGACCACAAT GCTGGCCAGT CCCGGGCCAG TCTTTCTCAT
    TCCTCCCATT CCTCCTATTC TTCCAGCAAG AAGAGCTCAT CAGTTCACTC CATCCCATCA
    ACAGCAAACC GACCGAACCG CACCAAGACC AGGGATTCCC GGGAAAAACA GAATGCAACA
    AGGATGAATA GAATGGGCCA AGCAGAAAAG AAATGGTTTA CAGATGAACC GGATAATGCC
    TATCCCAGAA ACATTCAAAT CAAGCCCATG AGCACCCACA TGGCCAATCA GATCAATCAA
    TACAAATCGA CAAGCAGCTT GATACCACCA ATCAGAGAAG TTGAAGATGA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37424
    Clone ID Related Accession (Same CDS sequence) XM_015288151.1 , XM_015288151.2
    Accession Version XM_015288151.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3825bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GTAAATGATT CAAATGTTCA GTTCTTGGAC
    CAAGATGATG ATGATGATCC AGATACAGAA CTGTACCTCA CGCAGCCCTT TGCATGTGGA
    ACCGCATTTG CTGTCAGCGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT
    ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG
    ATTGCCGAGG AAAATGCTTT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG
    GACAGGTGTC GAGTTGCTCA GTTGGCTTTG TATGATGGGC CATTTGCAGA CCTAGGGGAT
    GGAGGCTGCT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT
    GGAATTTATC GATTAAGGGA CGCTCATCTA AGTACTCCCA GCCAATGCAC TAAACGTTAT
    GTTATCACAA ACCCACCATA TGAGTTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG
    ATGCAATTCG ACCACAATGC TGGCCAGTCC CGGGCCAGTC TTTCTCATTC CTCCCATTCC
    TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCCA TCCCATCAAC AGCAAACCGA
    CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA ATGCAACAAG GATGAATAGA
    ATGGGCCAAG CAGAAAAGAA ATGGTTTACA GATGAACCGG ATAATGCCTA TCCCAGAAAC
    ATTCAAATCA AGCCCATGAG CACCCACATG GCCAATCAGA TCAATCAATA CAAATCGACA
    AGCAGCTTGA TACCACCAAT CAGAGAAGTT GAAGATGAAT GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143637.1
    CDS374..4198
    Translation

    Target ORF information:

    RefSeq Version XM_015288151.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288151.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GTAAATGATT CAAATGTTCA GTTCTTGGAC
    CAAGATGATG ATGATGATCC AGATACAGAA CTGTACCTCA CGCAGCCCTT TGCATGTGGA
    ACCGCATTTG CTGTCAGCGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT
    ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG
    ATTGCCGAGG AAAATGCTTT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG
    GACAGGTGTC GAGTTGCTCA GTTGGCTTTG TATGATGGGC CATTTGCAGA CCTAGGGGAT
    GGAGGCTGCT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT
    GGAATTTATC GATTAAGGGA CGCTCATCTA AGTACTCCCA GCCAATGCAC TAAACGTTAT
    GTTATCACAA ACCCACCATA TGAGTTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG
    ATGCAATTCG ACCACAATGC TGGCCAGTCC CGGGCCAGTC TTTCTCATTC CTCCCATTCC
    TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCCA TCCCATCAAC AGCAAACCGA
    CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA ATGCAACAAG GATGAATAGA
    ATGGGCCAAG CAGAAAAGAA ATGGTTTACA GATGAACCGG ATAATGCCTA TCCCAGAAAC
    ATTCAAATCA AGCCCATGAG CACCCACATG GCCAATCAGA TCAATCAATA CAAATCGACA
    AGCAGCTTGA TACCACCAAT CAGAGAAGTT GAAGATGAAT GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37424
    Clone ID Related Accession (Same CDS sequence) XM_015288151.1 , XM_015288151.2
    Accession Version XM_015288151.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3825bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288151.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GTAAATGATT CAAATGTTCA GTTCTTGGAC
    CAAGATGATG ATGATGATCC AGATACAGAA CTGTACCTCA CGCAGCCCTT TGCATGTGGA
    ACCGCATTTG CTGTCAGCGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT
    ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG
    ATTGCCGAGG AAAATGCTTT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG
    GACAGGTGTC GAGTTGCTCA GTTGGCTTTG TATGATGGGC CATTTGCAGA CCTAGGGGAT
    GGAGGCTGCT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT
    GGAATTTATC GATTAAGGGA CGCTCATCTA AGTACTCCCA GCCAATGCAC TAAACGTTAT
    GTTATCACAA ACCCACCATA TGAGTTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG
    ATGCAATTCG ACCACAATGC TGGCCAGTCC CGGGCCAGTC TTTCTCATTC CTCCCATTCC
    TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCCA TCCCATCAAC AGCAAACCGA
    CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA ATGCAACAAG GATGAATAGA
    ATGGGCCAAG CAGAAAAGAA ATGGTTTACA GATGAACCGG ATAATGCCTA TCCCAGAAAC
    ATTCAAATCA AGCCCATGAG CACCCACATG GCCAATCAGA TCAATCAATA CAAATCGACA
    AGCAGCTTGA TACCACCAAT CAGAGAAGTT GAAGATGAAT GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143637.1
    CDS369..4193
    Translation

    Target ORF information:

    RefSeq Version XM_015288151.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288151.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GTAAATGATT CAAATGTTCA GTTCTTGGAC
    CAAGATGATG ATGATGATCC AGATACAGAA CTGTACCTCA CGCAGCCCTT TGCATGTGGA
    ACCGCATTTG CTGTCAGCGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT
    ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG
    ATTGCCGAGG AAAATGCTTT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG
    GACAGGTGTC GAGTTGCTCA GTTGGCTTTG TATGATGGGC CATTTGCAGA CCTAGGGGAT
    GGAGGCTGCT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT
    GGAATTTATC GATTAAGGGA CGCTCATCTA AGTACTCCCA GCCAATGCAC TAAACGTTAT
    GTTATCACAA ACCCACCATA TGAGTTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG
    ATGCAATTCG ACCACAATGC TGGCCAGTCC CGGGCCAGTC TTTCTCATTC CTCCCATTCC
    TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCCA TCCCATCAAC AGCAAACCGA
    CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA ATGCAACAAG GATGAATAGA
    ATGGGCCAAG CAGAAAAGAA ATGGTTTACA GATGAACCGG ATAATGCCTA TCCCAGAAAC
    ATTCAAATCA AGCCCATGAG CACCCACATG GCCAATCAGA TCAATCAATA CAAATCGACA
    AGCAGCTTGA TACCACCAAT CAGAGAAGTT GAAGATGAAT GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37425
    Clone ID Related Accession (Same CDS sequence) XM_015288152.1 , XM_015288152.2
    Accession Version XM_015288152.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3822bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GTAAATGATT CAAATGTTCA GTTCTTGGAC
    CAAGATGATG ATGATGATCC AGATACAGAA CTGTACCTCA CGCAGCCCTT TGCATGTGGA
    ACCGCATTTG CTGTCAGCGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT
    ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG
    ATTGCCGAGG AAAATGCTTT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG
    GACAGGTGTC GAGTTGCTCA GTTGGCTTTG TATGATGGGC CATTTGCAGA CCTAGGGGAT
    GGAGGCTGCT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT
    GGAATTTATC GATTAAGGGA CGCTCATCTA AGTACTCCCA GCCAATGCAC TAAACGTTAT
    GTTATCACAA ACCCACCATA TGAGTTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG
    ATGCAATTCG ACCACAATGC TGGCCAGTCC CGGGCCAGTC TTTCTCATTC CTCCCATTCC
    TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCCA TCCCATCAAC AGCAAACCGA
    CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA ATGCAACAAG GATGAATAGA
    ATGGGCCAAG AAAAGAAATG GTTTACAGAT GAACCGGATA ATGCCTATCC CAGAAACATT
    CAAATCAAGC CCATGAGCAC CCACATGGCC AATCAGATCA ATCAATACAA ATCGACAAGC
    AGCTTGATAC CACCAATCAG AGAAGTTGAA GATGAATGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143638.1
    CDS374..4195
    Translation

    Target ORF information:

    RefSeq Version XM_015288152.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288152.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GTAAATGATT CAAATGTTCA GTTCTTGGAC
    CAAGATGATG ATGATGATCC AGATACAGAA CTGTACCTCA CGCAGCCCTT TGCATGTGGA
    ACCGCATTTG CTGTCAGCGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT
    ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG
    ATTGCCGAGG AAAATGCTTT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG
    GACAGGTGTC GAGTTGCTCA GTTGGCTTTG TATGATGGGC CATTTGCAGA CCTAGGGGAT
    GGAGGCTGCT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT
    GGAATTTATC GATTAAGGGA CGCTCATCTA AGTACTCCCA GCCAATGCAC TAAACGTTAT
    GTTATCACAA ACCCACCATA TGAGTTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG
    ATGCAATTCG ACCACAATGC TGGCCAGTCC CGGGCCAGTC TTTCTCATTC CTCCCATTCC
    TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCCA TCCCATCAAC AGCAAACCGA
    CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA ATGCAACAAG GATGAATAGA
    ATGGGCCAAG AAAAGAAATG GTTTACAGAT GAACCGGATA ATGCCTATCC CAGAAACATT
    CAAATCAAGC CCATGAGCAC CCACATGGCC AATCAGATCA ATCAATACAA ATCGACAAGC
    AGCTTGATAC CACCAATCAG AGAAGTTGAA GATGAATGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37425
    Clone ID Related Accession (Same CDS sequence) XM_015288152.1 , XM_015288152.2
    Accession Version XM_015288152.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3822bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288152.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GTAAATGATT CAAATGTTCA GTTCTTGGAC
    CAAGATGATG ATGATGATCC AGATACAGAA CTGTACCTCA CGCAGCCCTT TGCATGTGGA
    ACCGCATTTG CTGTCAGCGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT
    ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG
    ATTGCCGAGG AAAATGCTTT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG
    GACAGGTGTC GAGTTGCTCA GTTGGCTTTG TATGATGGGC CATTTGCAGA CCTAGGGGAT
    GGAGGCTGCT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT
    GGAATTTATC GATTAAGGGA CGCTCATCTA AGTACTCCCA GCCAATGCAC TAAACGTTAT
    GTTATCACAA ACCCACCATA TGAGTTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG
    ATGCAATTCG ACCACAATGC TGGCCAGTCC CGGGCCAGTC TTTCTCATTC CTCCCATTCC
    TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCCA TCCCATCAAC AGCAAACCGA
    CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA ATGCAACAAG GATGAATAGA
    ATGGGCCAAG AAAAGAAATG GTTTACAGAT GAACCGGATA ATGCCTATCC CAGAAACATT
    CAAATCAAGC CCATGAGCAC CCACATGGCC AATCAGATCA ATCAATACAA ATCGACAAGC
    AGCTTGATAC CACCAATCAG AGAAGTTGAA GATGAATGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143638.1
    CDS369..4190
    Translation

    Target ORF information:

    RefSeq Version XM_015288152.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288152.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GTAAATGATT CAAATGTTCA GTTCTTGGAC
    CAAGATGATG ATGATGATCC AGATACAGAA CTGTACCTCA CGCAGCCCTT TGCATGTGGA
    ACCGCATTTG CTGTCAGCGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT
    ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG
    ATTGCCGAGG AAAATGCTTT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG
    GACAGGTGTC GAGTTGCTCA GTTGGCTTTG TATGATGGGC CATTTGCAGA CCTAGGGGAT
    GGAGGCTGCT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT
    GGAATTTATC GATTAAGGGA CGCTCATCTA AGTACTCCCA GCCAATGCAC TAAACGTTAT
    GTTATCACAA ACCCACCATA TGAGTTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG
    ATGCAATTCG ACCACAATGC TGGCCAGTCC CGGGCCAGTC TTTCTCATTC CTCCCATTCC
    TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCCA TCCCATCAAC AGCAAACCGA
    CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA ATGCAACAAG GATGAATAGA
    ATGGGCCAAG AAAAGAAATG GTTTACAGAT GAACCGGATA ATGCCTATCC CAGAAACATT
    CAAATCAAGC CCATGAGCAC CCACATGGCC AATCAGATCA ATCAATACAA ATCGACAAGC
    AGCTTGATAC CACCAATCAG AGAAGTTGAA GATGAATGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37426
    Clone ID Related Accession (Same CDS sequence) XM_015288153.2 , XM_015288153.1
    Accession Version XM_015288153.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288153.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTCCAAG
    ATGTCCATCT ACAAGAGAAT GAAACTGGCA TGTTGTTTTG ATTGCGGACG CTCTGAGCGT
    GACTGCTCTT GCATGTCAGG CAGTGTACAT AGTAACATGG ACACCCTTGA GAGAGCCTTC
    CCACTTTCTT CTGTCTCTGT TAATGATTGC TCCACCAGTT TACGTGCCTT TGAAGATGAG
    CAGCCGTCAA CACTATCACC CAAAAAAAAG CAACGAAATG GAGGCATGCG AAATTCACCC
    AACTCCTCAC CCAAACTGAT GAGGCATGAC CCCTTGTTAA TTCCTGGCAA TGAACAGATT
    GACAATATGG ATGCCAATGT GAAAAAATAT GACTCTACGG GAATGTTTCA TTGGTGTCCA
    GCCAAGGATA TTGAAAAGGT CATTTTGACT CGAAGTGAAG CAGCGATGAC AGTTTTAAGT
    GGGCATGTAG TCGTTTGCAT ATTTGGGGAT GTTAAATCTG CTTTGATTGG ATTAAGAAAT
    TTAGTGATGC CATTACGAGC CAGCAACTTT CACTACCATG AACTCAAGCA CATTGTGTTT
    GTGGGTTCAC TTGAATACCT GAGAAGGGAA TGGGAGACCC TGCATAACTT CCCCAAGGTT
    TCAATCTTGC CTGGTACACC ATTAAGTCGG GCTGATTTAA GGGCTGTCAA CATCAACCTC
    TGCGACATGT GCGTTATCCT GTCAGCCAAT CAGAATAATA TTGATGATGC TTCGCTGCAG
    GACAAGGAAT GCATCTTGGC GTCACTCAAC ATCAAATCTA TGCAGTTTGA TGACAGCATT
    GGGGTCTTGC AGGCTAATTC CCAAGGCTTT ACACCCCCTG GTATGGATAG GTCATCTCCA
    GACAACAGTC CTGTCCACGG CCTGTTACGT CAACCCTCCA TTACAACAGG AGCCAACATC
    CCTATTATAA CAGAGCTAGT AAATGATTCA AATGTTCAGT TCTTGGACCA AGATGATGAT
    GATGATCCAG ATACAGAACT GTACCTCACG CAGCCCTTTG CATGTGGAAC CGCATTTGCT
    GTCAGCGTGC TGGACTCTCT CATGAGCGCA ACATACTTCA ATGACAATAT CCTCACACTG
    ATACGGACAT TGGTAACAGG AGGAGCCACA CCAGAGCTGG AAGCACTGAT TGCCGAGGAA
    AATGCTTTGA GAGGAGGTTA CAGCACGCCC CAGACACTTG CAAACAGGGA CAGGTGTCGA
    GTTGCTCAGT TGGCTTTGTA TGATGGGCCA TTTGCAGACC TAGGGGATGG AGGCTGCTAT
    GGAGATCTAT TTTGTAAAGC ACTGAAAACA TACAATATGC TTTGCTTTGG AATTTATCGA
    TTAAGGGACG CTCATCTAAG TACTCCCAGC CAATGCACTA AACGTTATGT TATCACAAAC
    CCACCATATG AGTTTGAGCT TGTGCCGACA GACTTGATCT TCTGTTTGAT GCAATTCGAC
    CACAATGCTG GCCAGTCCCG GGCCAGTCTT TCTCATTCCT CCCATTCCTC CTATTCTTCC
    AGCAAGAAGA GCTCATCAGT TCACTCCATC CCATCAACAG CAAACCGACC GAACCGCACC
    AAGACCAGGG ATTCCCGGGA AAAACAGAAT GCAACAAGGA TGAATAGAAT GGGCCAAGCA
    GAAAAGAAAT GGTTTACAGA TGAACCGGAT AATGCCTATC CCAGAAACAT TCAAATCAAG
    CCCATGAGCA CCCACATGGC CAATCAGATC AATCAATACA AATCGACAAG CAGCTTGATA
    CCACCAATCA GAGAAGTTGA AGATGAATGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143639.1
    CDS116..3928
    Translation

    Target ORF information:

    RefSeq Version XM_015288153.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288153.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTCCAAG
    ATGTCCATCT ACAAGAGAAT GAAACTGGCA TGTTGTTTTG ATTGCGGACG CTCTGAGCGT
    GACTGCTCTT GCATGTCAGG CAGTGTACAT AGTAACATGG ACACCCTTGA GAGAGCCTTC
    CCACTTTCTT CTGTCTCTGT TAATGATTGC TCCACCAGTT TACGTGCCTT TGAAGATGAG
    CAGCCGTCAA CACTATCACC CAAAAAAAAG CAACGAAATG GAGGCATGCG AAATTCACCC
    AACTCCTCAC CCAAACTGAT GAGGCATGAC CCCTTGTTAA TTCCTGGCAA TGAACAGATT
    GACAATATGG ATGCCAATGT GAAAAAATAT GACTCTACGG GAATGTTTCA TTGGTGTCCA
    GCCAAGGATA TTGAAAAGGT CATTTTGACT CGAAGTGAAG CAGCGATGAC AGTTTTAAGT
    GGGCATGTAG TCGTTTGCAT ATTTGGGGAT GTTAAATCTG CTTTGATTGG ATTAAGAAAT
    TTAGTGATGC CATTACGAGC CAGCAACTTT CACTACCATG AACTCAAGCA CATTGTGTTT
    GTGGGTTCAC TTGAATACCT GAGAAGGGAA TGGGAGACCC TGCATAACTT CCCCAAGGTT
    TCAATCTTGC CTGGTACACC ATTAAGTCGG GCTGATTTAA GGGCTGTCAA CATCAACCTC
    TGCGACATGT GCGTTATCCT GTCAGCCAAT CAGAATAATA TTGATGATGC TTCGCTGCAG
    GACAAGGAAT GCATCTTGGC GTCACTCAAC ATCAAATCTA TGCAGTTTGA TGACAGCATT
    GGGGTCTTGC AGGCTAATTC CCAAGGCTTT ACACCCCCTG GTATGGATAG GTCATCTCCA
    GACAACAGTC CTGTCCACGG CCTGTTACGT CAACCCTCCA TTACAACAGG AGCCAACATC
    CCTATTATAA CAGAGCTAGT AAATGATTCA AATGTTCAGT TCTTGGACCA AGATGATGAT
    GATGATCCAG ATACAGAACT GTACCTCACG CAGCCCTTTG CATGTGGAAC CGCATTTGCT
    GTCAGCGTGC TGGACTCTCT CATGAGCGCA ACATACTTCA ATGACAATAT CCTCACACTG
    ATACGGACAT TGGTAACAGG AGGAGCCACA CCAGAGCTGG AAGCACTGAT TGCCGAGGAA
    AATGCTTTGA GAGGAGGTTA CAGCACGCCC CAGACACTTG CAAACAGGGA CAGGTGTCGA
    GTTGCTCAGT TGGCTTTGTA TGATGGGCCA TTTGCAGACC TAGGGGATGG AGGCTGCTAT
    GGAGATCTAT TTTGTAAAGC ACTGAAAACA TACAATATGC TTTGCTTTGG AATTTATCGA
    TTAAGGGACG CTCATCTAAG TACTCCCAGC CAATGCACTA AACGTTATGT TATCACAAAC
    CCACCATATG AGTTTGAGCT TGTGCCGACA GACTTGATCT TCTGTTTGAT GCAATTCGAC
    CACAATGCTG GCCAGTCCCG GGCCAGTCTT TCTCATTCCT CCCATTCCTC CTATTCTTCC
    AGCAAGAAGA GCTCATCAGT TCACTCCATC CCATCAACAG CAAACCGACC GAACCGCACC
    AAGACCAGGG ATTCCCGGGA AAAACAGAAT GCAACAAGGA TGAATAGAAT GGGCCAAGCA
    GAAAAGAAAT GGTTTACAGA TGAACCGGAT AATGCCTATC CCAGAAACAT TCAAATCAAG
    CCCATGAGCA CCCACATGGC CAATCAGATC AATCAATACA AATCGACAAG CAGCTTGATA
    CCACCAATCA GAGAAGTTGA AGATGAATGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37426
    Clone ID Related Accession (Same CDS sequence) XM_015288153.2 , XM_015288153.1
    Accession Version XM_015288153.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTCCAAG
    ATGTCCATCT ACAAGAGAAT GAAACTGGCA TGTTGTTTTG ATTGCGGACG CTCTGAGCGT
    GACTGCTCTT GCATGTCAGG CAGTGTACAT AGTAACATGG ACACCCTTGA GAGAGCCTTC
    CCACTTTCTT CTGTCTCTGT TAATGATTGC TCCACCAGTT TACGTGCCTT TGAAGATGAG
    CAGCCGTCAA CACTATCACC CAAAAAAAAG CAACGAAATG GAGGCATGCG AAATTCACCC
    AACTCCTCAC CCAAACTGAT GAGGCATGAC CCCTTGTTAA TTCCTGGCAA TGAACAGATT
    GACAATATGG ATGCCAATGT GAAAAAATAT GACTCTACGG GAATGTTTCA TTGGTGTCCA
    GCCAAGGATA TTGAAAAGGT CATTTTGACT CGAAGTGAAG CAGCGATGAC AGTTTTAAGT
    GGGCATGTAG TCGTTTGCAT ATTTGGGGAT GTTAAATCTG CTTTGATTGG ATTAAGAAAT
    TTAGTGATGC CATTACGAGC CAGCAACTTT CACTACCATG AACTCAAGCA CATTGTGTTT
    GTGGGTTCAC TTGAATACCT GAGAAGGGAA TGGGAGACCC TGCATAACTT CCCCAAGGTT
    TCAATCTTGC CTGGTACACC ATTAAGTCGG GCTGATTTAA GGGCTGTCAA CATCAACCTC
    TGCGACATGT GCGTTATCCT GTCAGCCAAT CAGAATAATA TTGATGATGC TTCGCTGCAG
    GACAAGGAAT GCATCTTGGC GTCACTCAAC ATCAAATCTA TGCAGTTTGA TGACAGCATT
    GGGGTCTTGC AGGCTAATTC CCAAGGCTTT ACACCCCCTG GTATGGATAG GTCATCTCCA
    GACAACAGTC CTGTCCACGG CCTGTTACGT CAACCCTCCA TTACAACAGG AGCCAACATC
    CCTATTATAA CAGAGCTAGT AAATGATTCA AATGTTCAGT TCTTGGACCA AGATGATGAT
    GATGATCCAG ATACAGAACT GTACCTCACG CAGCCCTTTG CATGTGGAAC CGCATTTGCT
    GTCAGCGTGC TGGACTCTCT CATGAGCGCA ACATACTTCA ATGACAATAT CCTCACACTG
    ATACGGACAT TGGTAACAGG AGGAGCCACA CCAGAGCTGG AAGCACTGAT TGCCGAGGAA
    AATGCTTTGA GAGGAGGTTA CAGCACGCCC CAGACACTTG CAAACAGGGA CAGGTGTCGA
    GTTGCTCAGT TGGCTTTGTA TGATGGGCCA TTTGCAGACC TAGGGGATGG AGGCTGCTAT
    GGAGATCTAT TTTGTAAAGC ACTGAAAACA TACAATATGC TTTGCTTTGG AATTTATCGA
    TTAAGGGACG CTCATCTAAG TACTCCCAGC CAATGCACTA AACGTTATGT TATCACAAAC
    CCACCATATG AGTTTGAGCT TGTGCCGACA GACTTGATCT TCTGTTTGAT GCAATTCGAC
    CACAATGCTG GCCAGTCCCG GGCCAGTCTT TCTCATTCCT CCCATTCCTC CTATTCTTCC
    AGCAAGAAGA GCTCATCAGT TCACTCCATC CCATCAACAG CAAACCGACC GAACCGCACC
    AAGACCAGGG ATTCCCGGGA AAAACAGAAT GCAACAAGGA TGAATAGAAT GGGCCAAGCA
    GAAAAGAAAT GGTTTACAGA TGAACCGGAT AATGCCTATC CCAGAAACAT TCAAATCAAG
    CCCATGAGCA CCCACATGGC CAATCAGATC AATCAATACA AATCGACAAG CAGCTTGATA
    CCACCAATCA GAGAAGTTGA AGATGAATGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143639.1
    CDS116..3928
    Translation

    Target ORF information:

    RefSeq Version XM_015288153.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288153.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTCCAAG
    ATGTCCATCT ACAAGAGAAT GAAACTGGCA TGTTGTTTTG ATTGCGGACG CTCTGAGCGT
    GACTGCTCTT GCATGTCAGG CAGTGTACAT AGTAACATGG ACACCCTTGA GAGAGCCTTC
    CCACTTTCTT CTGTCTCTGT TAATGATTGC TCCACCAGTT TACGTGCCTT TGAAGATGAG
    CAGCCGTCAA CACTATCACC CAAAAAAAAG CAACGAAATG GAGGCATGCG AAATTCACCC
    AACTCCTCAC CCAAACTGAT GAGGCATGAC CCCTTGTTAA TTCCTGGCAA TGAACAGATT
    GACAATATGG ATGCCAATGT GAAAAAATAT GACTCTACGG GAATGTTTCA TTGGTGTCCA
    GCCAAGGATA TTGAAAAGGT CATTTTGACT CGAAGTGAAG CAGCGATGAC AGTTTTAAGT
    GGGCATGTAG TCGTTTGCAT ATTTGGGGAT GTTAAATCTG CTTTGATTGG ATTAAGAAAT
    TTAGTGATGC CATTACGAGC CAGCAACTTT CACTACCATG AACTCAAGCA CATTGTGTTT
    GTGGGTTCAC TTGAATACCT GAGAAGGGAA TGGGAGACCC TGCATAACTT CCCCAAGGTT
    TCAATCTTGC CTGGTACACC ATTAAGTCGG GCTGATTTAA GGGCTGTCAA CATCAACCTC
    TGCGACATGT GCGTTATCCT GTCAGCCAAT CAGAATAATA TTGATGATGC TTCGCTGCAG
    GACAAGGAAT GCATCTTGGC GTCACTCAAC ATCAAATCTA TGCAGTTTGA TGACAGCATT
    GGGGTCTTGC AGGCTAATTC CCAAGGCTTT ACACCCCCTG GTATGGATAG GTCATCTCCA
    GACAACAGTC CTGTCCACGG CCTGTTACGT CAACCCTCCA TTACAACAGG AGCCAACATC
    CCTATTATAA CAGAGCTAGT AAATGATTCA AATGTTCAGT TCTTGGACCA AGATGATGAT
    GATGATCCAG ATACAGAACT GTACCTCACG CAGCCCTTTG CATGTGGAAC CGCATTTGCT
    GTCAGCGTGC TGGACTCTCT CATGAGCGCA ACATACTTCA ATGACAATAT CCTCACACTG
    ATACGGACAT TGGTAACAGG AGGAGCCACA CCAGAGCTGG AAGCACTGAT TGCCGAGGAA
    AATGCTTTGA GAGGAGGTTA CAGCACGCCC CAGACACTTG CAAACAGGGA CAGGTGTCGA
    GTTGCTCAGT TGGCTTTGTA TGATGGGCCA TTTGCAGACC TAGGGGATGG AGGCTGCTAT
    GGAGATCTAT TTTGTAAAGC ACTGAAAACA TACAATATGC TTTGCTTTGG AATTTATCGA
    TTAAGGGACG CTCATCTAAG TACTCCCAGC CAATGCACTA AACGTTATGT TATCACAAAC
    CCACCATATG AGTTTGAGCT TGTGCCGACA GACTTGATCT TCTGTTTGAT GCAATTCGAC
    CACAATGCTG GCCAGTCCCG GGCCAGTCTT TCTCATTCCT CCCATTCCTC CTATTCTTCC
    AGCAAGAAGA GCTCATCAGT TCACTCCATC CCATCAACAG CAAACCGACC GAACCGCACC
    AAGACCAGGG ATTCCCGGGA AAAACAGAAT GCAACAAGGA TGAATAGAAT GGGCCAAGCA
    GAAAAGAAAT GGTTTACAGA TGAACCGGAT AATGCCTATC CCAGAAACAT TCAAATCAAG
    CCCATGAGCA CCCACATGGC CAATCAGATC AATCAATACA AATCGACAAG CAGCTTGATA
    CCACCAATCA GAGAAGTTGA AGATGAATGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37427
    Clone ID Related Accession (Same CDS sequence) XM_015288154.1
    Accession Version XM_015288154.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3801bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCCCAA GATGTCCATC
    TACAAGAGAA TGAAACTGGC ATGTTGTTTT GATTGCGGAC GCTCTGAGCG TGACTGCTCT
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACACCCTTG AGAGAGCCTT CCCACTTTCT
    TCTGTCTCTG TTAATGATTG CTCCACCAGT TTACGTGCCT TTGAAGATGA GCAGCCGTCA
    ACACTATCAC CCAAAAAAAA GCAACGAAAT GGAGGCATGC GAAATTCACC CAACTCCTCA
    CCCAAACTGA TGAGGCATGA CCCCTTGTTA ATTCCTGGCA ATGAACAGAT TGACAATATG
    GATGCCAATG TGAAAAAATA TGACTCTACG GGAATGTTTC ATTGGTGTCC AGCCAAGGAT
    ATTGAAAAGG TCATTTTGAC TCGAAGTGAA GCAGCGATGA CAGTTTTAAG TGGGCATGTA
    GTCGTTTGCA TATTTGGGGA TGTTAAATCT GCTTTGATTG GATTAAGAAA TTTAGTGATG
    CCATTACGAG CCAGCAACTT TCACTACCAT GAACTCAAGC ACATTGTGTT TGTGGGTTCA
    CTTGAATACC TGAGAAGGGA ATGGGAGACC CTGCATAACT TCCCCAAGGT TTCAATCTTG
    CCTGGTACAC CATTAAGTCG GGCTGATTTA AGGGCTGTCA ACATCAACCT CTGCGACATG
    TGCGTTATCC TGTCAGCCAA TCAGAATAAT ATTGATGATG CTTCGCTGCA GGACAAGGAA
    TGCATCTTGG CGTCACTCAA CATCAAATCT ATGCAGTTTG ATGACAGCAT TGGGGTCTTG
    CAGGCTAATT CCCAAGGCTT TACACCCCCT GGTATGGATA GGTCATCTCC AGACAACAGT
    CCTGTCCACG GCCTGTTACG TCAACCCTCC ATTACAACAG GAGCCAACAT CCCTATTATA
    ACAGAGCTAG TAAATGATTC AAATGTTCAG TTCTTGGACC AAGATGATGA TGATGATCCA
    GATACAGAAC TGTACCTCAC GCAGCCCTTT GCATGTGGAA CCGCATTTGC TGTCAGCGTG
    CTGGACTCTC TCATGAGCGC AACATACTTC AATGACAATA TCCTCACACT GATACGGACA
    TTGGTAACAG GAGGAGCCAC ACCAGAGCTG GAAGCACTGA TTGCCGAGGA AAATGCTTTG
    AGAGGAGGTT ACAGCACGCC CCAGACACTT GCAAACAGGG ACAGGTGTCG AGTTGCTCAG
    TTGGCTTTGT ATGATGGGCC ATTTGCAGAC CTAGGGGATG GAGGCTGCTA TGGAGATCTA
    TTTTGTAAAG CACTGAAAAC ATACAATATG CTTTGCTTTG GAATTTATCG ATTAAGGGAC
    GCTCATCTAA GTACTCCCAG CCAATGCACT AAACGTTATG TTATCACAAA CCCACCATAT
    GAGTTTGAGC TTGTGCCGAC AGACTTGATC TTCTGTTTGA TGCAATTCGA CCACAATGCT
    GGCCAGTCCC GGGCCAGTCT TTCTCATTCC TCCCATTCCT CCTATTCTTC CAGCAAGAAG
    AGCTCATCAG TTCACTCCAT CCCATCAACA GCAAACCGAC CGAACCGCAC CAAGACCAGG
    GATTCCCGGG AAAAACAGAA TGCAACAAGG ATGAATAGAA TGGGCCAAGA AAAGAAATGG
    TTTACAGATG AACCGGATAA TGCCTATCCC AGAAACATTC AAATCAAGCC CATGAGCACC
    CACATGGCCA ATCAGATCAA TCAATACAAA TCGACAAGCA GCTTGATACC ACCAATCAGA
    GAAGTTGAAG ATGAATGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143640.1
    CDS116..3916
    Translation

    Target ORF information:

    RefSeq Version XM_015288154.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288154.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCCCAA GATGTCCATC
    TACAAGAGAA TGAAACTGGC ATGTTGTTTT GATTGCGGAC GCTCTGAGCG TGACTGCTCT
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACACCCTTG AGAGAGCCTT CCCACTTTCT
    TCTGTCTCTG TTAATGATTG CTCCACCAGT TTACGTGCCT TTGAAGATGA GCAGCCGTCA
    ACACTATCAC CCAAAAAAAA GCAACGAAAT GGAGGCATGC GAAATTCACC CAACTCCTCA
    CCCAAACTGA TGAGGCATGA CCCCTTGTTA ATTCCTGGCA ATGAACAGAT TGACAATATG
    GATGCCAATG TGAAAAAATA TGACTCTACG GGAATGTTTC ATTGGTGTCC AGCCAAGGAT
    ATTGAAAAGG TCATTTTGAC TCGAAGTGAA GCAGCGATGA CAGTTTTAAG TGGGCATGTA
    GTCGTTTGCA TATTTGGGGA TGTTAAATCT GCTTTGATTG GATTAAGAAA TTTAGTGATG
    CCATTACGAG CCAGCAACTT TCACTACCAT GAACTCAAGC ACATTGTGTT TGTGGGTTCA
    CTTGAATACC TGAGAAGGGA ATGGGAGACC CTGCATAACT TCCCCAAGGT TTCAATCTTG
    CCTGGTACAC CATTAAGTCG GGCTGATTTA AGGGCTGTCA ACATCAACCT CTGCGACATG
    TGCGTTATCC TGTCAGCCAA TCAGAATAAT ATTGATGATG CTTCGCTGCA GGACAAGGAA
    TGCATCTTGG CGTCACTCAA CATCAAATCT ATGCAGTTTG ATGACAGCAT TGGGGTCTTG
    CAGGCTAATT CCCAAGGCTT TACACCCCCT GGTATGGATA GGTCATCTCC AGACAACAGT
    CCTGTCCACG GCCTGTTACG TCAACCCTCC ATTACAACAG GAGCCAACAT CCCTATTATA
    ACAGAGCTAG TAAATGATTC AAATGTTCAG TTCTTGGACC AAGATGATGA TGATGATCCA
    GATACAGAAC TGTACCTCAC GCAGCCCTTT GCATGTGGAA CCGCATTTGC TGTCAGCGTG
    CTGGACTCTC TCATGAGCGC AACATACTTC AATGACAATA TCCTCACACT GATACGGACA
    TTGGTAACAG GAGGAGCCAC ACCAGAGCTG GAAGCACTGA TTGCCGAGGA AAATGCTTTG
    AGAGGAGGTT ACAGCACGCC CCAGACACTT GCAAACAGGG ACAGGTGTCG AGTTGCTCAG
    TTGGCTTTGT ATGATGGGCC ATTTGCAGAC CTAGGGGATG GAGGCTGCTA TGGAGATCTA
    TTTTGTAAAG CACTGAAAAC ATACAATATG CTTTGCTTTG GAATTTATCG ATTAAGGGAC
    GCTCATCTAA GTACTCCCAG CCAATGCACT AAACGTTATG TTATCACAAA CCCACCATAT
    GAGTTTGAGC TTGTGCCGAC AGACTTGATC TTCTGTTTGA TGCAATTCGA CCACAATGCT
    GGCCAGTCCC GGGCCAGTCT TTCTCATTCC TCCCATTCCT CCTATTCTTC CAGCAAGAAG
    AGCTCATCAG TTCACTCCAT CCCATCAACA GCAAACCGAC CGAACCGCAC CAAGACCAGG
    GATTCCCGGG AAAAACAGAA TGCAACAAGG ATGAATAGAA TGGGCCAAGA AAAGAAATGG
    TTTACAGATG AACCGGATAA TGCCTATCCC AGAAACATTC AAATCAAGCC CATGAGCACC
    CACATGGCCA ATCAGATCAA TCAATACAAA TCGACAAGCA GCTTGATACC ACCAATCAGA
    GAAGTTGAAG ATGAATGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37428
    Clone ID Related Accession (Same CDS sequence) XM_015288155.1 , XM_015288155.2
    Accession Version XM_015288155.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3783bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGCGC TCATCATCCC GGTGACCATG GAGGTGCCCT GCGATAGCCG CGGACAGCGC 
    ATGTGGTGGG CTTTCCTCGC CTCATCCATG GTCACTTTCT TTGGGGGACT GTTTATCATT
    CTGCTGTGGA GGACCCTCAA GTACCTGTGG ACTGTCTGCT GCCACTGCGG GGTCAAAAAC
    AAGGAGGCCC AGAAGATTAA TGGTGGTGGA GATACGCAGG CAGATGGGGC CTGCAAACCA
    ACAGATGAAA AAGAGGAGAA CGTGGCAGCA GAAGTGGGAT GGATGACCTC CGTGAAAGAC
    TGGGCAGGAG TCATGATATC TGCCCAGACA CTAACTGGCA GAGTACTTGT TGTCTTAGTC
    TTCGCTCTTA GTATTGGTGC TCTTGTAATA TACTTCATAG ATTCATCAAA CCCAATAGAA
    TCCTGCCAGA ATTTCTACAA AGATTTCACA TTACAGATTG ACATGGCTTT CAATGTGTTC
    TTCCTACTCT ACTTCGGCTT GCGTTTCATA GCAGCCAATG ACAAGCTATG GTTTTGGCTT
    GAAGTCAACT CAGTAGTAGA TTTCTTCACG GTCCCTCCAG TGTTTGTTTC AGTATATTTA
    AACAGAAGTT GGCTTGGTTT AAGATTTTTA AGAGCCCTTA GACTGATACA GTTTTCAGAA
    ATATTGCAGT TTCTGAATAT CCTTAAAACA AGTAATTCCA TCAAGCTAGT GAATCTGTGC
    TCTATATTTA TCAGCACATG GCTGACAGCA GCTGGGTTCA TACATTTGGT GGAGAACTCA
    GGGGATCCTT GGGAAAATTT CCAAAATAAT CAACAGTTAA CATATTGGGA ATGTGTTTAT
    TTACTGATGG TTACAATGTC CACTGTTGGT TATGGAGATG TTTATGCAAA AACAACCCTT
    GGTCGCCTTT TCATGGTCTT CTTCATCCTT GGGGGATTGG CCATGTTTGC CAGCTACGTC
    CCTGAAATCA TAGAGTTAAT AGGAAACCGC AAGAAATATG GTGGTTCCTA TAGTGCGGTT
    AGTGGAAGAA AGCACATAGT TGTCTGTGGT CACATAACAC TTGAAAGCGT GTCCAACTTC
    CTGAAGGACT TCCTGCACAA GGATAGGGAT GATGTTAACG TAGAAATCGT CTTTCTGCAT
    AATATCTCCC CTAACCTTGA GCTGGAAGCT CTTTTTAAAC GACACTTCAC TCAGGTGGAA
    TTTTATCAGG GTTCAGTCCT TAATCCCCAT GACCTGGCAA GAGTAAAGAT AGAGTCAGCA
    GATGCGTGCC TAATTCTTGC CAATAAATAC TGCGCTGACC CAGATGCTGA GGATGCCTCC
    AATATTATGA GAGTTATTTC AATAAAGAAT TATCATCCGA AGATAAGAAT TATCACTCAG
    ATGTTGCAGT ATCACAATAA GGCTCATCTG TTAAATATTC CAAGCTGGAA TTGGAAGGAA
    GGGGATGATG CGATCTGTCT TGCTGAGCTC AAGCTGGGTT TCATAGCTCA GAGTTGCCTG
    GCACCAGGCC TTTCAACAAT GTTAGCCAAC CTCTTTTCCA TGAGATCATT CATAAAGATT
    GAGGAGGATA CATGGCAGAA ATACTATCTG GAAGGAGTTG CAAATGAAAT GTATACAGAA
    TATCTGTCTA GTGCCTTTGT GGGTTTGTCC TTCCCTGCAG TTTGTGAACT GGTGTTTGCG
    AAACTAAAGC TCTTAATGAT AGCCATAGAA TACAAGTCGG AAAAAAGAGA AAGCAGAAGC
    CGAAAGCGTA TATTGATCAA TCCTGGAAAC CATGTGAAGA TTCAAGAAGG TACCTTAGGA
    TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT TTTTTTACTG CAAGGCCTGC
    CATGATGACA TCACAGATCC CAAAAGGATA AAAAAGTGTG GCTGCAAACG GCTGATCTAT
    TCCAAGATGT CCATCTACAA GAGAATGAAA CTGGCATGTT GTTTTGATTG CGGACGCTCT
    GAGCGTGACT GCTCTTGCAT GTCAGGCAGT GTACATAGTA ACATGGACAC CCTTGAGAGA
    GCCTTCCCAC TTTCTTCTGT CTCTGTTAAT GATTGCTCCA CCAGTTTACG TGCCTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGCTAAG CCTGGCAAAC TTCTGCCTTT GGTTTCAATC
    AGTCAGGAAA AAAACAGTGG AACCCACATT CTAATGATAA CTGAACTGGT AAATGATTCA
    AATGTTCAGT TCTTGGACCA AGATGATGAT GATGATCCAG ATACAGAACT GTACCTCACG
    CAGCCCTTTG CATGTGGAAC CGCATTTGCT GTCAGCGTGC TGGACTCTCT CATGAGCGCA
    ACATACTTCA ATGACAATAT CCTCACACTG ATACGGACAT TGGTAACAGG AGGAGCCACA
    CCAGAGCTGG AAGCACTGAT TGCCGAGGAA AATGCTTTGA GAGGAGGTTA CAGCACGCCC
    CAGACACTTG CAAACAGGGA CAGGTGTCGA GTTGCTCAGT TGGCTTTGTA TGATGGGCCA
    TTTGCAGACC TAGGGGATGG AGGCTGCTAT GGAGATCTAT TTTGTAAAGC ACTGAAAACA
    TACAATATGC TTTGCTTTGG AATTTATCGA TTAAGGGACG CTCATCTAAG TACTCCCAGC
    CAATGCACTA AACGTTATGT TATCACAAAC CCACCATATG AGTTTGAGCT TGTGCCGACA
    GACTTGATCT TCTGTTTGAT GCAATTCGAC CACAATGCTG GCCAGTCCCG GGCCAGTCTT
    TCTCATTCCT CCCATTCCTC CTATTCTTCC AGCAAGAAGA GCTCATCAGT TCACTCCATC
    CCATCAACAG CAAACCGACC GAACCGCACC AAGACCAGGG ATTCCCGGGA AAAACAGAAT
    GCAACAAGGA TGAATAGAAT GGGCCAAGCA GAAAAGAAAT GGTTTACAGA TGAACCGGAT
    AATGCCTATC CCAGAAACAT TCAAATCAAG CCCATGAGCA CCCACATGGC CAATCAGATC
    AATCAATACA AATCGACAAG CAGCTTGATA CCACCAATCA GAGAAGTTGA AGATGAATGT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143641.1
    CDS187..3969
    Translation

    Target ORF information:

    RefSeq Version XM_015288155.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288155.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGCGC TCATCATCCC GGTGACCATG GAGGTGCCCT GCGATAGCCG CGGACAGCGC 
    ATGTGGTGGG CTTTCCTCGC CTCATCCATG GTCACTTTCT TTGGGGGACT GTTTATCATT
    CTGCTGTGGA GGACCCTCAA GTACCTGTGG ACTGTCTGCT GCCACTGCGG GGTCAAAAAC
    AAGGAGGCCC AGAAGATTAA TGGTGGTGGA GATACGCAGG CAGATGGGGC CTGCAAACCA
    ACAGATGAAA AAGAGGAGAA CGTGGCAGCA GAAGTGGGAT GGATGACCTC CGTGAAAGAC
    TGGGCAGGAG TCATGATATC TGCCCAGACA CTAACTGGCA GAGTACTTGT TGTCTTAGTC
    TTCGCTCTTA GTATTGGTGC TCTTGTAATA TACTTCATAG ATTCATCAAA CCCAATAGAA
    TCCTGCCAGA ATTTCTACAA AGATTTCACA TTACAGATTG ACATGGCTTT CAATGTGTTC
    TTCCTACTCT ACTTCGGCTT GCGTTTCATA GCAGCCAATG ACAAGCTATG GTTTTGGCTT
    GAAGTCAACT CAGTAGTAGA TTTCTTCACG GTCCCTCCAG TGTTTGTTTC AGTATATTTA
    AACAGAAGTT GGCTTGGTTT AAGATTTTTA AGAGCCCTTA GACTGATACA GTTTTCAGAA
    ATATTGCAGT TTCTGAATAT CCTTAAAACA AGTAATTCCA TCAAGCTAGT GAATCTGTGC
    TCTATATTTA TCAGCACATG GCTGACAGCA GCTGGGTTCA TACATTTGGT GGAGAACTCA
    GGGGATCCTT GGGAAAATTT CCAAAATAAT CAACAGTTAA CATATTGGGA ATGTGTTTAT
    TTACTGATGG TTACAATGTC CACTGTTGGT TATGGAGATG TTTATGCAAA AACAACCCTT
    GGTCGCCTTT TCATGGTCTT CTTCATCCTT GGGGGATTGG CCATGTTTGC CAGCTACGTC
    CCTGAAATCA TAGAGTTAAT AGGAAACCGC AAGAAATATG GTGGTTCCTA TAGTGCGGTT
    AGTGGAAGAA AGCACATAGT TGTCTGTGGT CACATAACAC TTGAAAGCGT GTCCAACTTC
    CTGAAGGACT TCCTGCACAA GGATAGGGAT GATGTTAACG TAGAAATCGT CTTTCTGCAT
    AATATCTCCC CTAACCTTGA GCTGGAAGCT CTTTTTAAAC GACACTTCAC TCAGGTGGAA
    TTTTATCAGG GTTCAGTCCT TAATCCCCAT GACCTGGCAA GAGTAAAGAT AGAGTCAGCA
    GATGCGTGCC TAATTCTTGC CAATAAATAC TGCGCTGACC CAGATGCTGA GGATGCCTCC
    AATATTATGA GAGTTATTTC AATAAAGAAT TATCATCCGA AGATAAGAAT TATCACTCAG
    ATGTTGCAGT ATCACAATAA GGCTCATCTG TTAAATATTC CAAGCTGGAA TTGGAAGGAA
    GGGGATGATG CGATCTGTCT TGCTGAGCTC AAGCTGGGTT TCATAGCTCA GAGTTGCCTG
    GCACCAGGCC TTTCAACAAT GTTAGCCAAC CTCTTTTCCA TGAGATCATT CATAAAGATT
    GAGGAGGATA CATGGCAGAA ATACTATCTG GAAGGAGTTG CAAATGAAAT GTATACAGAA
    TATCTGTCTA GTGCCTTTGT GGGTTTGTCC TTCCCTGCAG TTTGTGAACT GGTGTTTGCG
    AAACTAAAGC TCTTAATGAT AGCCATAGAA TACAAGTCGG AAAAAAGAGA AAGCAGAAGC
    CGAAAGCGTA TATTGATCAA TCCTGGAAAC CATGTGAAGA TTCAAGAAGG TACCTTAGGA
    TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT TTTTTTACTG CAAGGCCTGC
    CATGATGACA TCACAGATCC CAAAAGGATA AAAAAGTGTG GCTGCAAACG GCTGATCTAT
    TCCAAGATGT CCATCTACAA GAGAATGAAA CTGGCATGTT GTTTTGATTG CGGACGCTCT
    GAGCGTGACT GCTCTTGCAT GTCAGGCAGT GTACATAGTA ACATGGACAC CCTTGAGAGA
    GCCTTCCCAC TTTCTTCTGT CTCTGTTAAT GATTGCTCCA CCAGTTTACG TGCCTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGCTAAG CCTGGCAAAC TTCTGCCTTT GGTTTCAATC
    AGTCAGGAAA AAAACAGTGG AACCCACATT CTAATGATAA CTGAACTGGT AAATGATTCA
    AATGTTCAGT TCTTGGACCA AGATGATGAT GATGATCCAG ATACAGAACT GTACCTCACG
    CAGCCCTTTG CATGTGGAAC CGCATTTGCT GTCAGCGTGC TGGACTCTCT CATGAGCGCA
    ACATACTTCA ATGACAATAT CCTCACACTG ATACGGACAT TGGTAACAGG AGGAGCCACA
    CCAGAGCTGG AAGCACTGAT TGCCGAGGAA AATGCTTTGA GAGGAGGTTA CAGCACGCCC
    CAGACACTTG CAAACAGGGA CAGGTGTCGA GTTGCTCAGT TGGCTTTGTA TGATGGGCCA
    TTTGCAGACC TAGGGGATGG AGGCTGCTAT GGAGATCTAT TTTGTAAAGC ACTGAAAACA
    TACAATATGC TTTGCTTTGG AATTTATCGA TTAAGGGACG CTCATCTAAG TACTCCCAGC
    CAATGCACTA AACGTTATGT TATCACAAAC CCACCATATG AGTTTGAGCT TGTGCCGACA
    GACTTGATCT TCTGTTTGAT GCAATTCGAC CACAATGCTG GCCAGTCCCG GGCCAGTCTT
    TCTCATTCCT CCCATTCCTC CTATTCTTCC AGCAAGAAGA GCTCATCAGT TCACTCCATC
    CCATCAACAG CAAACCGACC GAACCGCACC AAGACCAGGG ATTCCCGGGA AAAACAGAAT
    GCAACAAGGA TGAATAGAAT GGGCCAAGCA GAAAAGAAAT GGTTTACAGA TGAACCGGAT
    AATGCCTATC CCAGAAACAT TCAAATCAAG CCCATGAGCA CCCACATGGC CAATCAGATC
    AATCAATACA AATCGACAAG CAGCTTGATA CCACCAATCA GAGAAGTTGA AGATGAATGT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37428
    Clone ID Related Accession (Same CDS sequence) XM_015288155.1 , XM_015288155.2
    Accession Version XM_015288155.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3783bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288155.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGCGC TCATCATCCC GGTGACCATG GAGGTGCCCT GCGATAGCCG CGGACAGCGC 
    ATGTGGTGGG CTTTCCTCGC CTCATCCATG GTCACTTTCT TTGGGGGACT GTTTATCATT
    CTGCTGTGGA GGACCCTCAA GTACCTGTGG ACTGTCTGCT GCCACTGCGG GGTCAAAAAC
    AAGGAGGCCC AGAAGATTAA TGGTGGTGGA GATACGCAGG CAGATGGGGC CTGCAAACCA
    ACAGATGAAA AAGAGGAGAA CGTGGCAGCA GAAGTGGGAT GGATGACCTC CGTGAAAGAC
    TGGGCAGGAG TCATGATATC TGCCCAGACA CTAACTGGCA GAGTACTTGT TGTCTTAGTC
    TTCGCTCTTA GTATTGGTGC TCTTGTAATA TACTTCATAG ATTCATCAAA CCCAATAGAA
    TCCTGCCAGA ATTTCTACAA AGATTTCACA TTACAGATTG ACATGGCTTT CAATGTGTTC
    TTCCTACTCT ACTTCGGCTT GCGTTTCATA GCAGCCAATG ACAAGCTATG GTTTTGGCTT
    GAAGTCAACT CAGTAGTAGA TTTCTTCACG GTCCCTCCAG TGTTTGTTTC AGTATATTTA
    AACAGAAGTT GGCTTGGTTT AAGATTTTTA AGAGCCCTTA GACTGATACA GTTTTCAGAA
    ATATTGCAGT TTCTGAATAT CCTTAAAACA AGTAATTCCA TCAAGCTAGT GAATCTGTGC
    TCTATATTTA TCAGCACATG GCTGACAGCA GCTGGGTTCA TACATTTGGT GGAGAACTCA
    GGGGATCCTT GGGAAAATTT CCAAAATAAT CAACAGTTAA CATATTGGGA ATGTGTTTAT
    TTACTGATGG TTACAATGTC CACTGTTGGT TATGGAGATG TTTATGCAAA AACAACCCTT
    GGTCGCCTTT TCATGGTCTT CTTCATCCTT GGGGGATTGG CCATGTTTGC CAGCTACGTC
    CCTGAAATCA TAGAGTTAAT AGGAAACCGC AAGAAATATG GTGGTTCCTA TAGTGCGGTT
    AGTGGAAGAA AGCACATAGT TGTCTGTGGT CACATAACAC TTGAAAGCGT GTCCAACTTC
    CTGAAGGACT TCCTGCACAA GGATAGGGAT GATGTTAACG TAGAAATCGT CTTTCTGCAT
    AATATCTCCC CTAACCTTGA GCTGGAAGCT CTTTTTAAAC GACACTTCAC TCAGGTGGAA
    TTTTATCAGG GTTCAGTCCT TAATCCCCAT GACCTGGCAA GAGTAAAGAT AGAGTCAGCA
    GATGCGTGCC TAATTCTTGC CAATAAATAC TGCGCTGACC CAGATGCTGA GGATGCCTCC
    AATATTATGA GAGTTATTTC AATAAAGAAT TATCATCCGA AGATAAGAAT TATCACTCAG
    ATGTTGCAGT ATCACAATAA GGCTCATCTG TTAAATATTC CAAGCTGGAA TTGGAAGGAA
    GGGGATGATG CGATCTGTCT TGCTGAGCTC AAGCTGGGTT TCATAGCTCA GAGTTGCCTG
    GCACCAGGCC TTTCAACAAT GTTAGCCAAC CTCTTTTCCA TGAGATCATT CATAAAGATT
    GAGGAGGATA CATGGCAGAA ATACTATCTG GAAGGAGTTG CAAATGAAAT GTATACAGAA
    TATCTGTCTA GTGCCTTTGT GGGTTTGTCC TTCCCTGCAG TTTGTGAACT GGTGTTTGCG
    AAACTAAAGC TCTTAATGAT AGCCATAGAA TACAAGTCGG AAAAAAGAGA AAGCAGAAGC
    CGAAAGCGTA TATTGATCAA TCCTGGAAAC CATGTGAAGA TTCAAGAAGG TACCTTAGGA
    TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT TTTTTTACTG CAAGGCCTGC
    CATGATGACA TCACAGATCC CAAAAGGATA AAAAAGTGTG GCTGCAAACG GCTGATCTAT
    TCCAAGATGT CCATCTACAA GAGAATGAAA CTGGCATGTT GTTTTGATTG CGGACGCTCT
    GAGCGTGACT GCTCTTGCAT GTCAGGCAGT GTACATAGTA ACATGGACAC CCTTGAGAGA
    GCCTTCCCAC TTTCTTCTGT CTCTGTTAAT GATTGCTCCA CCAGTTTACG TGCCTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGCTAAG CCTGGCAAAC TTCTGCCTTT GGTTTCAATC
    AGTCAGGAAA AAAACAGTGG AACCCACATT CTAATGATAA CTGAACTGGT AAATGATTCA
    AATGTTCAGT TCTTGGACCA AGATGATGAT GATGATCCAG ATACAGAACT GTACCTCACG
    CAGCCCTTTG CATGTGGAAC CGCATTTGCT GTCAGCGTGC TGGACTCTCT CATGAGCGCA
    ACATACTTCA ATGACAATAT CCTCACACTG ATACGGACAT TGGTAACAGG AGGAGCCACA
    CCAGAGCTGG AAGCACTGAT TGCCGAGGAA AATGCTTTGA GAGGAGGTTA CAGCACGCCC
    CAGACACTTG CAAACAGGGA CAGGTGTCGA GTTGCTCAGT TGGCTTTGTA TGATGGGCCA
    TTTGCAGACC TAGGGGATGG AGGCTGCTAT GGAGATCTAT TTTGTAAAGC ACTGAAAACA
    TACAATATGC TTTGCTTTGG AATTTATCGA TTAAGGGACG CTCATCTAAG TACTCCCAGC
    CAATGCACTA AACGTTATGT TATCACAAAC CCACCATATG AGTTTGAGCT TGTGCCGACA
    GACTTGATCT TCTGTTTGAT GCAATTCGAC CACAATGCTG GCCAGTCCCG GGCCAGTCTT
    TCTCATTCCT CCCATTCCTC CTATTCTTCC AGCAAGAAGA GCTCATCAGT TCACTCCATC
    CCATCAACAG CAAACCGACC GAACCGCACC AAGACCAGGG ATTCCCGGGA AAAACAGAAT
    GCAACAAGGA TGAATAGAAT GGGCCAAGCA GAAAAGAAAT GGTTTACAGA TGAACCGGAT
    AATGCCTATC CCAGAAACAT TCAAATCAAG CCCATGAGCA CCCACATGGC CAATCAGATC
    AATCAATACA AATCGACAAG CAGCTTGATA CCACCAATCA GAGAAGTTGA AGATGAATGT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143641.1
    CDS168..3950
    Translation

    Target ORF information:

    RefSeq Version XM_015288155.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288155.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGCGC TCATCATCCC GGTGACCATG GAGGTGCCCT GCGATAGCCG CGGACAGCGC 
    ATGTGGTGGG CTTTCCTCGC CTCATCCATG GTCACTTTCT TTGGGGGACT GTTTATCATT
    CTGCTGTGGA GGACCCTCAA GTACCTGTGG ACTGTCTGCT GCCACTGCGG GGTCAAAAAC
    AAGGAGGCCC AGAAGATTAA TGGTGGTGGA GATACGCAGG CAGATGGGGC CTGCAAACCA
    ACAGATGAAA AAGAGGAGAA CGTGGCAGCA GAAGTGGGAT GGATGACCTC CGTGAAAGAC
    TGGGCAGGAG TCATGATATC TGCCCAGACA CTAACTGGCA GAGTACTTGT TGTCTTAGTC
    TTCGCTCTTA GTATTGGTGC TCTTGTAATA TACTTCATAG ATTCATCAAA CCCAATAGAA
    TCCTGCCAGA ATTTCTACAA AGATTTCACA TTACAGATTG ACATGGCTTT CAATGTGTTC
    TTCCTACTCT ACTTCGGCTT GCGTTTCATA GCAGCCAATG ACAAGCTATG GTTTTGGCTT
    GAAGTCAACT CAGTAGTAGA TTTCTTCACG GTCCCTCCAG TGTTTGTTTC AGTATATTTA
    AACAGAAGTT GGCTTGGTTT AAGATTTTTA AGAGCCCTTA GACTGATACA GTTTTCAGAA
    ATATTGCAGT TTCTGAATAT CCTTAAAACA AGTAATTCCA TCAAGCTAGT GAATCTGTGC
    TCTATATTTA TCAGCACATG GCTGACAGCA GCTGGGTTCA TACATTTGGT GGAGAACTCA
    GGGGATCCTT GGGAAAATTT CCAAAATAAT CAACAGTTAA CATATTGGGA ATGTGTTTAT
    TTACTGATGG TTACAATGTC CACTGTTGGT TATGGAGATG TTTATGCAAA AACAACCCTT
    GGTCGCCTTT TCATGGTCTT CTTCATCCTT GGGGGATTGG CCATGTTTGC CAGCTACGTC
    CCTGAAATCA TAGAGTTAAT AGGAAACCGC AAGAAATATG GTGGTTCCTA TAGTGCGGTT
    AGTGGAAGAA AGCACATAGT TGTCTGTGGT CACATAACAC TTGAAAGCGT GTCCAACTTC
    CTGAAGGACT TCCTGCACAA GGATAGGGAT GATGTTAACG TAGAAATCGT CTTTCTGCAT
    AATATCTCCC CTAACCTTGA GCTGGAAGCT CTTTTTAAAC GACACTTCAC TCAGGTGGAA
    TTTTATCAGG GTTCAGTCCT TAATCCCCAT GACCTGGCAA GAGTAAAGAT AGAGTCAGCA
    GATGCGTGCC TAATTCTTGC CAATAAATAC TGCGCTGACC CAGATGCTGA GGATGCCTCC
    AATATTATGA GAGTTATTTC AATAAAGAAT TATCATCCGA AGATAAGAAT TATCACTCAG
    ATGTTGCAGT ATCACAATAA GGCTCATCTG TTAAATATTC CAAGCTGGAA TTGGAAGGAA
    GGGGATGATG CGATCTGTCT TGCTGAGCTC AAGCTGGGTT TCATAGCTCA GAGTTGCCTG
    GCACCAGGCC TTTCAACAAT GTTAGCCAAC CTCTTTTCCA TGAGATCATT CATAAAGATT
    GAGGAGGATA CATGGCAGAA ATACTATCTG GAAGGAGTTG CAAATGAAAT GTATACAGAA
    TATCTGTCTA GTGCCTTTGT GGGTTTGTCC TTCCCTGCAG TTTGTGAACT GGTGTTTGCG
    AAACTAAAGC TCTTAATGAT AGCCATAGAA TACAAGTCGG AAAAAAGAGA AAGCAGAAGC
    CGAAAGCGTA TATTGATCAA TCCTGGAAAC CATGTGAAGA TTCAAGAAGG TACCTTAGGA
    TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT TTTTTTACTG CAAGGCCTGC
    CATGATGACA TCACAGATCC CAAAAGGATA AAAAAGTGTG GCTGCAAACG GCTGATCTAT
    TCCAAGATGT CCATCTACAA GAGAATGAAA CTGGCATGTT GTTTTGATTG CGGACGCTCT
    GAGCGTGACT GCTCTTGCAT GTCAGGCAGT GTACATAGTA ACATGGACAC CCTTGAGAGA
    GCCTTCCCAC TTTCTTCTGT CTCTGTTAAT GATTGCTCCA CCAGTTTACG TGCCTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGCTAAG CCTGGCAAAC TTCTGCCTTT GGTTTCAATC
    AGTCAGGAAA AAAACAGTGG AACCCACATT CTAATGATAA CTGAACTGGT AAATGATTCA
    AATGTTCAGT TCTTGGACCA AGATGATGAT GATGATCCAG ATACAGAACT GTACCTCACG
    CAGCCCTTTG CATGTGGAAC CGCATTTGCT GTCAGCGTGC TGGACTCTCT CATGAGCGCA
    ACATACTTCA ATGACAATAT CCTCACACTG ATACGGACAT TGGTAACAGG AGGAGCCACA
    CCAGAGCTGG AAGCACTGAT TGCCGAGGAA AATGCTTTGA GAGGAGGTTA CAGCACGCCC
    CAGACACTTG CAAACAGGGA CAGGTGTCGA GTTGCTCAGT TGGCTTTGTA TGATGGGCCA
    TTTGCAGACC TAGGGGATGG AGGCTGCTAT GGAGATCTAT TTTGTAAAGC ACTGAAAACA
    TACAATATGC TTTGCTTTGG AATTTATCGA TTAAGGGACG CTCATCTAAG TACTCCCAGC
    CAATGCACTA AACGTTATGT TATCACAAAC CCACCATATG AGTTTGAGCT TGTGCCGACA
    GACTTGATCT TCTGTTTGAT GCAATTCGAC CACAATGCTG GCCAGTCCCG GGCCAGTCTT
    TCTCATTCCT CCCATTCCTC CTATTCTTCC AGCAAGAAGA GCTCATCAGT TCACTCCATC
    CCATCAACAG CAAACCGACC GAACCGCACC AAGACCAGGG ATTCCCGGGA AAAACAGAAT
    GCAACAAGGA TGAATAGAAT GGGCCAAGCA GAAAAGAAAT GGTTTACAGA TGAACCGGAT
    AATGCCTATC CCAGAAACAT TCAAATCAAG CCCATGAGCA CCCACATGGC CAATCAGATC
    AATCAATACA AATCGACAAG CAGCTTGATA CCACCAATCA GAGAAGTTGA AGATGAATGT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37429
    Clone ID Related Accession (Same CDS sequence) XM_015288156.1 , XM_015288156.2
    Accession Version XM_015288156.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GCTAAGCCTG GCAAACTTCT GCCTTTGGTT
    TCAATCAGTC AGGAAAAAAA CAGTGGAACC CACATTCTAA TGATAACTGA ACTGGTAAAT
    GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGATAC AGAACTGTAC
    CTCACGCAGC CCTTTGCATG TGGAACCGCA TTTGCTGTCA GCGTGCTGGA CTCTCTCATG
    AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA
    GCCACACCAG AGCTGGAAGC ACTGATTGCC GAGGAAAATG CTTTGAGAGG AGGTTACAGC
    ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGTTGGC TTTGTATGAT
    GGGCCATTTG CAGACCTAGG GGATGGAGGC TGCTATGGAG ATCTATTTTG TAAAGCACTG
    AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGACGCTCA TCTAAGTACT
    CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CATATGAGTT TGAGCTTGTG
    CCGACAGACT TGATCTTCTG TTTGATGCAA TTCGACCACA ATGCTGGCCA GTCCCGGGCC
    AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC
    TCCATCCCAT CAACAGCAAA CCGACCGAAC CGCACCAAGA CCAGGGATTC CCGGGAAAAA
    CAGAACAGAA AAGAAATGGT TTACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143642.1
    CDS374..4123
    Translation

    Target ORF information:

    RefSeq Version XM_015288156.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288156.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GCTAAGCCTG GCAAACTTCT GCCTTTGGTT
    TCAATCAGTC AGGAAAAAAA CAGTGGAACC CACATTCTAA TGATAACTGA ACTGGTAAAT
    GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGATAC AGAACTGTAC
    CTCACGCAGC CCTTTGCATG TGGAACCGCA TTTGCTGTCA GCGTGCTGGA CTCTCTCATG
    AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA
    GCCACACCAG AGCTGGAAGC ACTGATTGCC GAGGAAAATG CTTTGAGAGG AGGTTACAGC
    ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGTTGGC TTTGTATGAT
    GGGCCATTTG CAGACCTAGG GGATGGAGGC TGCTATGGAG ATCTATTTTG TAAAGCACTG
    AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGACGCTCA TCTAAGTACT
    CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CATATGAGTT TGAGCTTGTG
    CCGACAGACT TGATCTTCTG TTTGATGCAA TTCGACCACA ATGCTGGCCA GTCCCGGGCC
    AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC
    TCCATCCCAT CAACAGCAAA CCGACCGAAC CGCACCAAGA CCAGGGATTC CCGGGAAAAA
    CAGAACAGAA AAGAAATGGT TTACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37429
    Clone ID Related Accession (Same CDS sequence) XM_015288156.1 , XM_015288156.2
    Accession Version XM_015288156.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288156.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GCTAAGCCTG GCAAACTTCT GCCTTTGGTT
    TCAATCAGTC AGGAAAAAAA CAGTGGAACC CACATTCTAA TGATAACTGA ACTGGTAAAT
    GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGATAC AGAACTGTAC
    CTCACGCAGC CCTTTGCATG TGGAACCGCA TTTGCTGTCA GCGTGCTGGA CTCTCTCATG
    AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA
    GCCACACCAG AGCTGGAAGC ACTGATTGCC GAGGAAAATG CTTTGAGAGG AGGTTACAGC
    ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGTTGGC TTTGTATGAT
    GGGCCATTTG CAGACCTAGG GGATGGAGGC TGCTATGGAG ATCTATTTTG TAAAGCACTG
    AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGACGCTCA TCTAAGTACT
    CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CATATGAGTT TGAGCTTGTG
    CCGACAGACT TGATCTTCTG TTTGATGCAA TTCGACCACA ATGCTGGCCA GTCCCGGGCC
    AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC
    TCCATCCCAT CAACAGCAAA CCGACCGAAC CGCACCAAGA CCAGGGATTC CCGGGAAAAA
    CAGAACAGAA AAGAAATGGT TTACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143642.1
    CDS369..4118
    Translation

    Target ORF information:

    RefSeq Version XM_015288156.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288156.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GCTAAGCCTG GCAAACTTCT GCCTTTGGTT
    TCAATCAGTC AGGAAAAAAA CAGTGGAACC CACATTCTAA TGATAACTGA ACTGGTAAAT
    GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGATAC AGAACTGTAC
    CTCACGCAGC CCTTTGCATG TGGAACCGCA TTTGCTGTCA GCGTGCTGGA CTCTCTCATG
    AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA
    GCCACACCAG AGCTGGAAGC ACTGATTGCC GAGGAAAATG CTTTGAGAGG AGGTTACAGC
    ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGTTGGC TTTGTATGAT
    GGGCCATTTG CAGACCTAGG GGATGGAGGC TGCTATGGAG ATCTATTTTG TAAAGCACTG
    AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGACGCTCA TCTAAGTACT
    CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CATATGAGTT TGAGCTTGTG
    CCGACAGACT TGATCTTCTG TTTGATGCAA TTCGACCACA ATGCTGGCCA GTCCCGGGCC
    AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC
    TCCATCCCAT CAACAGCAAA CCGACCGAAC CGCACCAAGA CCAGGGATTC CCGGGAAAAA
    CAGAACAGAA AAGAAATGGT TTACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37430
    Clone ID Related Accession (Same CDS sequence) XM_015288157.1 , XM_015288157.2
    Accession Version XM_015288157.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3747bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GCTAAGCCTG GCAAACTTCT GCCTTTGGTT
    TCAATCAGTC AGGAAAAAAA CAGTGGAACC CACATTCTAA TGATAACTGA ACTGGTAAAT
    GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGATAC AGAACTGTAC
    CTCACGCAGC CCTTTGCATG TGGAACCGCA TTTGCTGTCA GCGTGCTGGA CTCTCTCATG
    AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA
    GCCACACCAG AGCTGGAAGC ACTGATTGCC GAGGAAAATG CTTTGAGAGG AGGTTACAGC
    ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGTTGGC TTTGTATGAT
    GGGCCATTTG CAGACCTAGG GGATGGAGGC TGCTATGGAG ATCTATTTTG TAAAGCACTG
    AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGACGCTCA TCTAAGTACT
    CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CATATGAGTT TGAGCTTGTG
    CCGACAGACT TGATCTTCTG TTTGATGCAA TTCGACCACA ATGCTGGCCA GTCCCGGGCC
    AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC
    TCCATCCCAT CAACAGCAAA CCGACCGAAC CGCACCAAGA CCAGGGATTC CCGGGAAAAA
    CAGAAAAAAG AAATGGTTTA CAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143643.1
    CDS374..4120
    Translation

    Target ORF information:

    RefSeq Version XM_015288157.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288157.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GCTAAGCCTG GCAAACTTCT GCCTTTGGTT
    TCAATCAGTC AGGAAAAAAA CAGTGGAACC CACATTCTAA TGATAACTGA ACTGGTAAAT
    GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGATAC AGAACTGTAC
    CTCACGCAGC CCTTTGCATG TGGAACCGCA TTTGCTGTCA GCGTGCTGGA CTCTCTCATG
    AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA
    GCCACACCAG AGCTGGAAGC ACTGATTGCC GAGGAAAATG CTTTGAGAGG AGGTTACAGC
    ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGTTGGC TTTGTATGAT
    GGGCCATTTG CAGACCTAGG GGATGGAGGC TGCTATGGAG ATCTATTTTG TAAAGCACTG
    AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGACGCTCA TCTAAGTACT
    CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CATATGAGTT TGAGCTTGTG
    CCGACAGACT TGATCTTCTG TTTGATGCAA TTCGACCACA ATGCTGGCCA GTCCCGGGCC
    AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC
    TCCATCCCAT CAACAGCAAA CCGACCGAAC CGCACCAAGA CCAGGGATTC CCGGGAAAAA
    CAGAAAAAAG AAATGGTTTA CAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37430
    Clone ID Related Accession (Same CDS sequence) XM_015288157.1 , XM_015288157.2
    Accession Version XM_015288157.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3747bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288157.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GCTAAGCCTG GCAAACTTCT GCCTTTGGTT
    TCAATCAGTC AGGAAAAAAA CAGTGGAACC CACATTCTAA TGATAACTGA ACTGGTAAAT
    GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGATAC AGAACTGTAC
    CTCACGCAGC CCTTTGCATG TGGAACCGCA TTTGCTGTCA GCGTGCTGGA CTCTCTCATG
    AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA
    GCCACACCAG AGCTGGAAGC ACTGATTGCC GAGGAAAATG CTTTGAGAGG AGGTTACAGC
    ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGTTGGC TTTGTATGAT
    GGGCCATTTG CAGACCTAGG GGATGGAGGC TGCTATGGAG ATCTATTTTG TAAAGCACTG
    AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGACGCTCA TCTAAGTACT
    CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CATATGAGTT TGAGCTTGTG
    CCGACAGACT TGATCTTCTG TTTGATGCAA TTCGACCACA ATGCTGGCCA GTCCCGGGCC
    AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC
    TCCATCCCAT CAACAGCAAA CCGACCGAAC CGCACCAAGA CCAGGGATTC CCGGGAAAAA
    CAGAAAAAAG AAATGGTTTA CAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143643.1
    CDS369..4115
    Translation

    Target ORF information:

    RefSeq Version XM_015288157.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288157.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GCTAAGCCTG GCAAACTTCT GCCTTTGGTT
    TCAATCAGTC AGGAAAAAAA CAGTGGAACC CACATTCTAA TGATAACTGA ACTGGTAAAT
    GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGATAC AGAACTGTAC
    CTCACGCAGC CCTTTGCATG TGGAACCGCA TTTGCTGTCA GCGTGCTGGA CTCTCTCATG
    AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA
    GCCACACCAG AGCTGGAAGC ACTGATTGCC GAGGAAAATG CTTTGAGAGG AGGTTACAGC
    ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGTTGGC TTTGTATGAT
    GGGCCATTTG CAGACCTAGG GGATGGAGGC TGCTATGGAG ATCTATTTTG TAAAGCACTG
    AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGACGCTCA TCTAAGTACT
    CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CATATGAGTT TGAGCTTGTG
    CCGACAGACT TGATCTTCTG TTTGATGCAA TTCGACCACA ATGCTGGCCA GTCCCGGGCC
    AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC
    TCCATCCCAT CAACAGCAAA CCGACCGAAC CGCACCAAGA CCAGGGATTC CCGGGAAAAA
    CAGAAAAAAG AAATGGTTTA CAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37431
    Clone ID Related Accession (Same CDS sequence) XM_015288158.1 , XM_015288158.2
    Accession Version XM_015288158.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3735bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTTTG AAGATGAGCA GCCGTCAACA CTATCACCCA AAAAAAAGCA ACGAAATGGA
    GGCATGCGAA ATTCACCCAA CTCCTCACCC AAACTGATGA GGCATGACCC CTTGTTAATT
    CCTGGCAATG AACAGATTGA CAATATGGAT GCCAATGTGA AAAAATATGA CTCTACGGGA
    ATGTTTCATT GGTGTCCAGC CAAGGATATT GAAAAGGTCA TTTTGACTCG AAGTGAAGCA
    GCGATGACAG TTTTAAGTGG GCATGTAGTC GTTTGCATAT TTGGGGATGT TAAATCTGCT
    TTGATTGGAT TAAGAAATTT AGTGATGCCA TTACGAGCCA GCAACTTTCA CTACCATGAA
    CTCAAGCACA TTGTGTTTGT GGGTTCACTT GAATACCTGA GAAGGGAATG GGAGACCCTG
    CATAACTTCC CCAAGGTTTC AATCTTGCCT GGTACACCAT TAAGTCGGGC TGATTTAAGG
    GCTGTCAACA TCAACCTCTG CGACATGTGC GTTATCCTGT CAGCCAATCA GAATAATATT
    GATGATGCTT CGCTGCAGGA CAAGGAATGC ATCTTGGCGT CACTCAACAT CAAATCTATG
    CAGTTTGATG ACAGCATTGG GGTCTTGCAG GCTAATTCCC AAGGCTTTAC ACCCCCTGGT
    ATGGATAGGT CATCTCCAGA CAACAGTCCT GTCCACGGCC TGTTACGTCA ACCCTCCATT
    ACAACAGGAG CCAACATCCC TATTATAACA GAGCTAGCTA AGCCTGGCAA ACTTCTGCCT
    TTGGTTTCAA TCAGTCAGGA AAAAAACAGT GGAACCCACA TTCTAATGAT AACTGAACTG
    GTAAATGATT CAAATGTTCA GTTCTTGGAC CAAGATGATG ATGATGATCC AGATACAGAA
    CTGTACCTCA CGCAGCCCTT TGCATGTGGA ACCGCATTTG CTGTCAGCGT GCTGGACTCT
    CTCATGAGCG CAACATACTT CAATGACAAT ATCCTCACAC TGATACGGAC ATTGGTAACA
    GGAGGAGCCA CACCAGAGCT GGAAGCACTG ATTGCCGAGG AAAATGCTTT GAGAGGAGGT
    TACAGCACGC CCCAGACACT TGCAAACAGG GACAGGTGTC GAGTTGCTCA GTTGGCTTTG
    TATGATGGGC CATTTGCAGA CCTAGGGGAT GGAGGCTGCT ATGGAGATCT ATTTTGTAAA
    GCACTGAAAA CATACAATAT GCTTTGCTTT GGAATTTATC GATTAAGGGA CGCTCATCTA
    AGTACTCCCA GCCAATGCAC TAAACGTTAT GTTATCACAA ACCCACCATA TGAGTTTGAG
    CTTGTGCCGA CAGACTTGAT CTTCTGTTTG ATGCAATTCG ACCACAATGC TGGCCAGTCC
    CGGGCCAGTC TTTCTCATTC CTCCCATTCC TCCTATTCTT CCAGCAAGAA GAGCTCATCA
    GTTCACTCCA TCCCATCAAC AGCAAACCGA CCGAACCGCA CCAAGACCAG GGATTCCCGG
    GAAAAACAGA ATGCAACAAG GATGAATAGA ATGGGCCAAG CAGAAAAGAA ATGGTTTACA
    GATGAACCGG ATAATGCCTA TCCCAGAAAC ATTCAAATCA AGCCCATGAG CACCCACATG
    GCCAATCAGA TCAATCAATA CAAATCGACA AGCAGCTTGA TACCACCAAT CAGAGAAGTT
    GAAGATGAAT GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143644.1
    CDS373..4107
    Translation

    Target ORF information:

    RefSeq Version XM_015288158.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288158.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTTTG AAGATGAGCA GCCGTCAACA CTATCACCCA AAAAAAAGCA ACGAAATGGA
    GGCATGCGAA ATTCACCCAA CTCCTCACCC AAACTGATGA GGCATGACCC CTTGTTAATT
    CCTGGCAATG AACAGATTGA CAATATGGAT GCCAATGTGA AAAAATATGA CTCTACGGGA
    ATGTTTCATT GGTGTCCAGC CAAGGATATT GAAAAGGTCA TTTTGACTCG AAGTGAAGCA
    GCGATGACAG TTTTAAGTGG GCATGTAGTC GTTTGCATAT TTGGGGATGT TAAATCTGCT
    TTGATTGGAT TAAGAAATTT AGTGATGCCA TTACGAGCCA GCAACTTTCA CTACCATGAA
    CTCAAGCACA TTGTGTTTGT GGGTTCACTT GAATACCTGA GAAGGGAATG GGAGACCCTG
    CATAACTTCC CCAAGGTTTC AATCTTGCCT GGTACACCAT TAAGTCGGGC TGATTTAAGG
    GCTGTCAACA TCAACCTCTG CGACATGTGC GTTATCCTGT CAGCCAATCA GAATAATATT
    GATGATGCTT CGCTGCAGGA CAAGGAATGC ATCTTGGCGT CACTCAACAT CAAATCTATG
    CAGTTTGATG ACAGCATTGG GGTCTTGCAG GCTAATTCCC AAGGCTTTAC ACCCCCTGGT
    ATGGATAGGT CATCTCCAGA CAACAGTCCT GTCCACGGCC TGTTACGTCA ACCCTCCATT
    ACAACAGGAG CCAACATCCC TATTATAACA GAGCTAGCTA AGCCTGGCAA ACTTCTGCCT
    TTGGTTTCAA TCAGTCAGGA AAAAAACAGT GGAACCCACA TTCTAATGAT AACTGAACTG
    GTAAATGATT CAAATGTTCA GTTCTTGGAC CAAGATGATG ATGATGATCC AGATACAGAA
    CTGTACCTCA CGCAGCCCTT TGCATGTGGA ACCGCATTTG CTGTCAGCGT GCTGGACTCT
    CTCATGAGCG CAACATACTT CAATGACAAT ATCCTCACAC TGATACGGAC ATTGGTAACA
    GGAGGAGCCA CACCAGAGCT GGAAGCACTG ATTGCCGAGG AAAATGCTTT GAGAGGAGGT
    TACAGCACGC CCCAGACACT TGCAAACAGG GACAGGTGTC GAGTTGCTCA GTTGGCTTTG
    TATGATGGGC CATTTGCAGA CCTAGGGGAT GGAGGCTGCT ATGGAGATCT ATTTTGTAAA
    GCACTGAAAA CATACAATAT GCTTTGCTTT GGAATTTATC GATTAAGGGA CGCTCATCTA
    AGTACTCCCA GCCAATGCAC TAAACGTTAT GTTATCACAA ACCCACCATA TGAGTTTGAG
    CTTGTGCCGA CAGACTTGAT CTTCTGTTTG ATGCAATTCG ACCACAATGC TGGCCAGTCC
    CGGGCCAGTC TTTCTCATTC CTCCCATTCC TCCTATTCTT CCAGCAAGAA GAGCTCATCA
    GTTCACTCCA TCCCATCAAC AGCAAACCGA CCGAACCGCA CCAAGACCAG GGATTCCCGG
    GAAAAACAGA ATGCAACAAG GATGAATAGA ATGGGCCAAG CAGAAAAGAA ATGGTTTACA
    GATGAACCGG ATAATGCCTA TCCCAGAAAC ATTCAAATCA AGCCCATGAG CACCCACATG
    GCCAATCAGA TCAATCAATA CAAATCGACA AGCAGCTTGA TACCACCAAT CAGAGAAGTT
    GAAGATGAAT GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37431
    Clone ID Related Accession (Same CDS sequence) XM_015288158.1 , XM_015288158.2
    Accession Version XM_015288158.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3735bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288158.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTTTG AAGATGAGCA GCCGTCAACA CTATCACCCA AAAAAAAGCA ACGAAATGGA
    GGCATGCGAA ATTCACCCAA CTCCTCACCC AAACTGATGA GGCATGACCC CTTGTTAATT
    CCTGGCAATG AACAGATTGA CAATATGGAT GCCAATGTGA AAAAATATGA CTCTACGGGA
    ATGTTTCATT GGTGTCCAGC CAAGGATATT GAAAAGGTCA TTTTGACTCG AAGTGAAGCA
    GCGATGACAG TTTTAAGTGG GCATGTAGTC GTTTGCATAT TTGGGGATGT TAAATCTGCT
    TTGATTGGAT TAAGAAATTT AGTGATGCCA TTACGAGCCA GCAACTTTCA CTACCATGAA
    CTCAAGCACA TTGTGTTTGT GGGTTCACTT GAATACCTGA GAAGGGAATG GGAGACCCTG
    CATAACTTCC CCAAGGTTTC AATCTTGCCT GGTACACCAT TAAGTCGGGC TGATTTAAGG
    GCTGTCAACA TCAACCTCTG CGACATGTGC GTTATCCTGT CAGCCAATCA GAATAATATT
    GATGATGCTT CGCTGCAGGA CAAGGAATGC ATCTTGGCGT CACTCAACAT CAAATCTATG
    CAGTTTGATG ACAGCATTGG GGTCTTGCAG GCTAATTCCC AAGGCTTTAC ACCCCCTGGT
    ATGGATAGGT CATCTCCAGA CAACAGTCCT GTCCACGGCC TGTTACGTCA ACCCTCCATT
    ACAACAGGAG CCAACATCCC TATTATAACA GAGCTAGCTA AGCCTGGCAA ACTTCTGCCT
    TTGGTTTCAA TCAGTCAGGA AAAAAACAGT GGAACCCACA TTCTAATGAT AACTGAACTG
    GTAAATGATT CAAATGTTCA GTTCTTGGAC CAAGATGATG ATGATGATCC AGATACAGAA
    CTGTACCTCA CGCAGCCCTT TGCATGTGGA ACCGCATTTG CTGTCAGCGT GCTGGACTCT
    CTCATGAGCG CAACATACTT CAATGACAAT ATCCTCACAC TGATACGGAC ATTGGTAACA
    GGAGGAGCCA CACCAGAGCT GGAAGCACTG ATTGCCGAGG AAAATGCTTT GAGAGGAGGT
    TACAGCACGC CCCAGACACT TGCAAACAGG GACAGGTGTC GAGTTGCTCA GTTGGCTTTG
    TATGATGGGC CATTTGCAGA CCTAGGGGAT GGAGGCTGCT ATGGAGATCT ATTTTGTAAA
    GCACTGAAAA CATACAATAT GCTTTGCTTT GGAATTTATC GATTAAGGGA CGCTCATCTA
    AGTACTCCCA GCCAATGCAC TAAACGTTAT GTTATCACAA ACCCACCATA TGAGTTTGAG
    CTTGTGCCGA CAGACTTGAT CTTCTGTTTG ATGCAATTCG ACCACAATGC TGGCCAGTCC
    CGGGCCAGTC TTTCTCATTC CTCCCATTCC TCCTATTCTT CCAGCAAGAA GAGCTCATCA
    GTTCACTCCA TCCCATCAAC AGCAAACCGA CCGAACCGCA CCAAGACCAG GGATTCCCGG
    GAAAAACAGA ATGCAACAAG GATGAATAGA ATGGGCCAAG CAGAAAAGAA ATGGTTTACA
    GATGAACCGG ATAATGCCTA TCCCAGAAAC ATTCAAATCA AGCCCATGAG CACCCACATG
    GCCAATCAGA TCAATCAATA CAAATCGACA AGCAGCTTGA TACCACCAAT CAGAGAAGTT
    GAAGATGAAT GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143644.1
    CDS369..4103
    Translation

    Target ORF information:

    RefSeq Version XM_015288158.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288158.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTTTG AAGATGAGCA GCCGTCAACA CTATCACCCA AAAAAAAGCA ACGAAATGGA
    GGCATGCGAA ATTCACCCAA CTCCTCACCC AAACTGATGA GGCATGACCC CTTGTTAATT
    CCTGGCAATG AACAGATTGA CAATATGGAT GCCAATGTGA AAAAATATGA CTCTACGGGA
    ATGTTTCATT GGTGTCCAGC CAAGGATATT GAAAAGGTCA TTTTGACTCG AAGTGAAGCA
    GCGATGACAG TTTTAAGTGG GCATGTAGTC GTTTGCATAT TTGGGGATGT TAAATCTGCT
    TTGATTGGAT TAAGAAATTT AGTGATGCCA TTACGAGCCA GCAACTTTCA CTACCATGAA
    CTCAAGCACA TTGTGTTTGT GGGTTCACTT GAATACCTGA GAAGGGAATG GGAGACCCTG
    CATAACTTCC CCAAGGTTTC AATCTTGCCT GGTACACCAT TAAGTCGGGC TGATTTAAGG
    GCTGTCAACA TCAACCTCTG CGACATGTGC GTTATCCTGT CAGCCAATCA GAATAATATT
    GATGATGCTT CGCTGCAGGA CAAGGAATGC ATCTTGGCGT CACTCAACAT CAAATCTATG
    CAGTTTGATG ACAGCATTGG GGTCTTGCAG GCTAATTCCC AAGGCTTTAC ACCCCCTGGT
    ATGGATAGGT CATCTCCAGA CAACAGTCCT GTCCACGGCC TGTTACGTCA ACCCTCCATT
    ACAACAGGAG CCAACATCCC TATTATAACA GAGCTAGCTA AGCCTGGCAA ACTTCTGCCT
    TTGGTTTCAA TCAGTCAGGA AAAAAACAGT GGAACCCACA TTCTAATGAT AACTGAACTG
    GTAAATGATT CAAATGTTCA GTTCTTGGAC CAAGATGATG ATGATGATCC AGATACAGAA
    CTGTACCTCA CGCAGCCCTT TGCATGTGGA ACCGCATTTG CTGTCAGCGT GCTGGACTCT
    CTCATGAGCG CAACATACTT CAATGACAAT ATCCTCACAC TGATACGGAC ATTGGTAACA
    GGAGGAGCCA CACCAGAGCT GGAAGCACTG ATTGCCGAGG AAAATGCTTT GAGAGGAGGT
    TACAGCACGC CCCAGACACT TGCAAACAGG GACAGGTGTC GAGTTGCTCA GTTGGCTTTG
    TATGATGGGC CATTTGCAGA CCTAGGGGAT GGAGGCTGCT ATGGAGATCT ATTTTGTAAA
    GCACTGAAAA CATACAATAT GCTTTGCTTT GGAATTTATC GATTAAGGGA CGCTCATCTA
    AGTACTCCCA GCCAATGCAC TAAACGTTAT GTTATCACAA ACCCACCATA TGAGTTTGAG
    CTTGTGCCGA CAGACTTGAT CTTCTGTTTG ATGCAATTCG ACCACAATGC TGGCCAGTCC
    CGGGCCAGTC TTTCTCATTC CTCCCATTCC TCCTATTCTT CCAGCAAGAA GAGCTCATCA
    GTTCACTCCA TCCCATCAAC AGCAAACCGA CCGAACCGCA CCAAGACCAG GGATTCCCGG
    GAAAAACAGA ATGCAACAAG GATGAATAGA ATGGGCCAAG CAGAAAAGAA ATGGTTTACA
    GATGAACCGG ATAATGCCTA TCCCAGAAAC ATTCAAATCA AGCCCATGAG CACCCACATG
    GCCAATCAGA TCAATCAATA CAAATCGACA AGCAGCTTGA TACCACCAAT CAGAGAAGTT
    GAAGATGAAT GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37432
    Clone ID Related Accession (Same CDS sequence) XM_015288159.2 , XM_015288159.1
    Accession Version XM_015288159.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3726bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288159.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTT
    GAAGATGAGC AGCCGTCAAC ACTATCACCC AAAAAAAAGC AACGAAATGG AGGCATGCGA
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAT
    GAACAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACGGG AATGTTTCAT
    TGGTGTCCAG CCAAGGATAT TGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACCCT GCATAACTTC
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGCTTTA CACCCCCTGG TATGGATAGG
    TCATCTCCAG ACAACAGTCC TGTCCACGGC CTGTTACGTC AACCCTCCAT TACAACAGGA
    GCCAACATCC CTATTATAAC AGAGCTAGCT AAGCCTGGCA AACTTCTGCC TTTGGTTTCA
    ATCAGTCAGG AAAAAAACAG TGGAACCCAC ATTCTAATGA TAACTGAACT GGTAAATGAT
    TCAAATGTTC AGTTCTTGGA CCAAGATGAT GATGATGATC CAGATACAGA ACTGTACCTC
    ACGCAGCCCT TTGCATGTGG AACCGCATTT GCTGTCAGCG TGCTGGACTC TCTCATGAGC
    GCAACATACT TCAATGACAA TATCCTCACA CTGATACGGA CATTGGTAAC AGGAGGAGCC
    ACACCAGAGC TGGAAGCACT GATTGCCGAG GAAAATGCTT TGAGAGGAGG TTACAGCACG
    CCCCAGACAC TTGCAAACAG GGACAGGTGT CGAGTTGCTC AGTTGGCTTT GTATGATGGG
    CCATTTGCAG ACCTAGGGGA TGGAGGCTGC TATGGAGATC TATTTTGTAA AGCACTGAAA
    ACATACAATA TGCTTTGCTT TGGAATTTAT CGATTAAGGG ACGCTCATCT AAGTACTCCC
    AGCCAATGCA CTAAACGTTA TGTTATCACA AACCCACCAT ATGAGTTTGA GCTTGTGCCG
    ACAGACTTGA TCTTCTGTTT GATGCAATTC GACCACAATG CTGGCCAGTC CCGGGCCAGT
    CTTTCTCATT CCTCCCATTC CTCCTATTCT TCCAGCAAGA AGAGCTCATC AGTTCACTCC
    ATCCCATCAA CAGCAAACCG ACCGAACCGC ACCAAGACCA GGGATTCCCG GGAAAAACAG
    AATGCAACAA GGATGAATAG AATGGGCCAA GCAGAAAAGA AATGGTTTAC AGATGAACCG
    GATAATGCCT ATCCCAGAAA CATTCAAATC AAGCCCATGA GCACCCACAT GGCCAATCAG
    ATCAATCAAT ACAAATCGAC AAGCAGCTTG ATACCACCAA TCAGAGAAGT TGAAGATGAA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143645.1
    CDS368..4093
    Translation

    Target ORF information:

    RefSeq Version XM_015288159.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288159.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTT
    GAAGATGAGC AGCCGTCAAC ACTATCACCC AAAAAAAAGC AACGAAATGG AGGCATGCGA
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAT
    GAACAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACGGG AATGTTTCAT
    TGGTGTCCAG CCAAGGATAT TGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACCCT GCATAACTTC
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGCTTTA CACCCCCTGG TATGGATAGG
    TCATCTCCAG ACAACAGTCC TGTCCACGGC CTGTTACGTC AACCCTCCAT TACAACAGGA
    GCCAACATCC CTATTATAAC AGAGCTAGCT AAGCCTGGCA AACTTCTGCC TTTGGTTTCA
    ATCAGTCAGG AAAAAAACAG TGGAACCCAC ATTCTAATGA TAACTGAACT GGTAAATGAT
    TCAAATGTTC AGTTCTTGGA CCAAGATGAT GATGATGATC CAGATACAGA ACTGTACCTC
    ACGCAGCCCT TTGCATGTGG AACCGCATTT GCTGTCAGCG TGCTGGACTC TCTCATGAGC
    GCAACATACT TCAATGACAA TATCCTCACA CTGATACGGA CATTGGTAAC AGGAGGAGCC
    ACACCAGAGC TGGAAGCACT GATTGCCGAG GAAAATGCTT TGAGAGGAGG TTACAGCACG
    CCCCAGACAC TTGCAAACAG GGACAGGTGT CGAGTTGCTC AGTTGGCTTT GTATGATGGG
    CCATTTGCAG ACCTAGGGGA TGGAGGCTGC TATGGAGATC TATTTTGTAA AGCACTGAAA
    ACATACAATA TGCTTTGCTT TGGAATTTAT CGATTAAGGG ACGCTCATCT AAGTACTCCC
    AGCCAATGCA CTAAACGTTA TGTTATCACA AACCCACCAT ATGAGTTTGA GCTTGTGCCG
    ACAGACTTGA TCTTCTGTTT GATGCAATTC GACCACAATG CTGGCCAGTC CCGGGCCAGT
    CTTTCTCATT CCTCCCATTC CTCCTATTCT TCCAGCAAGA AGAGCTCATC AGTTCACTCC
    ATCCCATCAA CAGCAAACCG ACCGAACCGC ACCAAGACCA GGGATTCCCG GGAAAAACAG
    AATGCAACAA GGATGAATAG AATGGGCCAA GCAGAAAAGA AATGGTTTAC AGATGAACCG
    GATAATGCCT ATCCCAGAAA CATTCAAATC AAGCCCATGA GCACCCACAT GGCCAATCAG
    ATCAATCAAT ACAAATCGAC AAGCAGCTTG ATACCACCAA TCAGAGAAGT TGAAGATGAA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37432
    Clone ID Related Accession (Same CDS sequence) XM_015288159.2 , XM_015288159.1
    Accession Version XM_015288159.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3726bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTT
    GAAGATGAGC AGCCGTCAAC ACTATCACCC AAAAAAAAGC AACGAAATGG AGGCATGCGA
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAT
    GAACAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACGGG AATGTTTCAT
    TGGTGTCCAG CCAAGGATAT TGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACCCT GCATAACTTC
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGCTTTA CACCCCCTGG TATGGATAGG
    TCATCTCCAG ACAACAGTCC TGTCCACGGC CTGTTACGTC AACCCTCCAT TACAACAGGA
    GCCAACATCC CTATTATAAC AGAGCTAGCT AAGCCTGGCA AACTTCTGCC TTTGGTTTCA
    ATCAGTCAGG AAAAAAACAG TGGAACCCAC ATTCTAATGA TAACTGAACT GGTAAATGAT
    TCAAATGTTC AGTTCTTGGA CCAAGATGAT GATGATGATC CAGATACAGA ACTGTACCTC
    ACGCAGCCCT TTGCATGTGG AACCGCATTT GCTGTCAGCG TGCTGGACTC TCTCATGAGC
    GCAACATACT TCAATGACAA TATCCTCACA CTGATACGGA CATTGGTAAC AGGAGGAGCC
    ACACCAGAGC TGGAAGCACT GATTGCCGAG GAAAATGCTT TGAGAGGAGG TTACAGCACG
    CCCCAGACAC TTGCAAACAG GGACAGGTGT CGAGTTGCTC AGTTGGCTTT GTATGATGGG
    CCATTTGCAG ACCTAGGGGA TGGAGGCTGC TATGGAGATC TATTTTGTAA AGCACTGAAA
    ACATACAATA TGCTTTGCTT TGGAATTTAT CGATTAAGGG ACGCTCATCT AAGTACTCCC
    AGCCAATGCA CTAAACGTTA TGTTATCACA AACCCACCAT ATGAGTTTGA GCTTGTGCCG
    ACAGACTTGA TCTTCTGTTT GATGCAATTC GACCACAATG CTGGCCAGTC CCGGGCCAGT
    CTTTCTCATT CCTCCCATTC CTCCTATTCT TCCAGCAAGA AGAGCTCATC AGTTCACTCC
    ATCCCATCAA CAGCAAACCG ACCGAACCGC ACCAAGACCA GGGATTCCCG GGAAAAACAG
    AATGCAACAA GGATGAATAG AATGGGCCAA GCAGAAAAGA AATGGTTTAC AGATGAACCG
    GATAATGCCT ATCCCAGAAA CATTCAAATC AAGCCCATGA GCACCCACAT GGCCAATCAG
    ATCAATCAAT ACAAATCGAC AAGCAGCTTG ATACCACCAA TCAGAGAAGT TGAAGATGAA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143645.1
    CDS373..4098
    Translation

    Target ORF information:

    RefSeq Version XM_015288159.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288159.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTT
    GAAGATGAGC AGCCGTCAAC ACTATCACCC AAAAAAAAGC AACGAAATGG AGGCATGCGA
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAT
    GAACAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACGGG AATGTTTCAT
    TGGTGTCCAG CCAAGGATAT TGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACCCT GCATAACTTC
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGCTTTA CACCCCCTGG TATGGATAGG
    TCATCTCCAG ACAACAGTCC TGTCCACGGC CTGTTACGTC AACCCTCCAT TACAACAGGA
    GCCAACATCC CTATTATAAC AGAGCTAGCT AAGCCTGGCA AACTTCTGCC TTTGGTTTCA
    ATCAGTCAGG AAAAAAACAG TGGAACCCAC ATTCTAATGA TAACTGAACT GGTAAATGAT
    TCAAATGTTC AGTTCTTGGA CCAAGATGAT GATGATGATC CAGATACAGA ACTGTACCTC
    ACGCAGCCCT TTGCATGTGG AACCGCATTT GCTGTCAGCG TGCTGGACTC TCTCATGAGC
    GCAACATACT TCAATGACAA TATCCTCACA CTGATACGGA CATTGGTAAC AGGAGGAGCC
    ACACCAGAGC TGGAAGCACT GATTGCCGAG GAAAATGCTT TGAGAGGAGG TTACAGCACG
    CCCCAGACAC TTGCAAACAG GGACAGGTGT CGAGTTGCTC AGTTGGCTTT GTATGATGGG
    CCATTTGCAG ACCTAGGGGA TGGAGGCTGC TATGGAGATC TATTTTGTAA AGCACTGAAA
    ACATACAATA TGCTTTGCTT TGGAATTTAT CGATTAAGGG ACGCTCATCT AAGTACTCCC
    AGCCAATGCA CTAAACGTTA TGTTATCACA AACCCACCAT ATGAGTTTGA GCTTGTGCCG
    ACAGACTTGA TCTTCTGTTT GATGCAATTC GACCACAATG CTGGCCAGTC CCGGGCCAGT
    CTTTCTCATT CCTCCCATTC CTCCTATTCT TCCAGCAAGA AGAGCTCATC AGTTCACTCC
    ATCCCATCAA CAGCAAACCG ACCGAACCGC ACCAAGACCA GGGATTCCCG GGAAAAACAG
    AATGCAACAA GGATGAATAG AATGGGCCAA GCAGAAAAGA AATGGTTTAC AGATGAACCG
    GATAATGCCT ATCCCAGAAA CATTCAAATC AAGCCCATGA GCACCCACAT GGCCAATCAG
    ATCAATCAAT ACAAATCGAC AAGCAGCTTG ATACCACCAA TCAGAGAAGT TGAAGATGAA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37433
    Clone ID Related Accession (Same CDS sequence) XM_015288160.1 , XM_015288160.2
    Accession Version XM_015288160.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3723bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGCTAAG CCTGGCAAAC TTCTGCCTTT GGTTTCAATC
    AGTCAGGAAA AAAACAGTGG AACCCACATT CTAATGATAA CTGAACTGGT AAATGATTCA
    AATGTTCAGT TCTTGGACCA AGATGATGAT GATGATCCAG ATACAGAACT GTACCTCACG
    CAGCCCTTTG CATGTGGAAC CGCATTTGCT GTCAGCGTGC TGGACTCTCT CATGAGCGCA
    ACATACTTCA ATGACAATAT CCTCACACTG ATACGGACAT TGGTAACAGG AGGAGCCACA
    CCAGAGCTGG AAGCACTGAT TGCCGAGGAA AATGCTTTGA GAGGAGGTTA CAGCACGCCC
    CAGACACTTG CAAACAGGGA CAGGTGTCGA GTTGCTCAGT TGGCTTTGTA TGATGGGCCA
    TTTGCAGACC TAGGGGATGG AGGCTGCTAT GGAGATCTAT TTTGTAAAGC ACTGAAAACA
    TACAATATGC TTTGCTTTGG AATTTATCGA TTAAGGGACG CTCATCTAAG TACTCCCAGC
    CAATGCACTA AACGTTATGT TATCACAAAC CCACCATATG AGTTTGAGCT TGTGCCGACA
    GACTTGATCT TCTGTTTGAT GCAATTCGAC CACAATGCTG GCCAGTCCCG GGCCAGTCTT
    TCTCATTCCT CCCATTCCTC CTATTCTTCC AGCAAGAAGA GCTCATCAGT TCACTCCATC
    CCATCAACAG CAAACCGACC GAACCGCACC AAGACCAGGG ATTCCCGGGA AAAACAGAAT
    GCAACAAGGA TGAATAGAAT GGGCCAAGCA GAAAAGAAAT GGTTTACAGA TGAACCGGAT
    AATGCCTATC CCAGAAACAT TCAAATCAAG CCCATGAGCA CCCACATGGC CAATCAGATC
    AATCAATACA AATCGACAAG CAGCTTGATA CCACCAATCA GAGAAGTTGA AGATGAATGT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143646.1
    CDS373..4095
    Translation

    Target ORF information:

    RefSeq Version XM_015288160.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288160.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGCTAAG CCTGGCAAAC TTCTGCCTTT GGTTTCAATC
    AGTCAGGAAA AAAACAGTGG AACCCACATT CTAATGATAA CTGAACTGGT AAATGATTCA
    AATGTTCAGT TCTTGGACCA AGATGATGAT GATGATCCAG ATACAGAACT GTACCTCACG
    CAGCCCTTTG CATGTGGAAC CGCATTTGCT GTCAGCGTGC TGGACTCTCT CATGAGCGCA
    ACATACTTCA ATGACAATAT CCTCACACTG ATACGGACAT TGGTAACAGG AGGAGCCACA
    CCAGAGCTGG AAGCACTGAT TGCCGAGGAA AATGCTTTGA GAGGAGGTTA CAGCACGCCC
    CAGACACTTG CAAACAGGGA CAGGTGTCGA GTTGCTCAGT TGGCTTTGTA TGATGGGCCA
    TTTGCAGACC TAGGGGATGG AGGCTGCTAT GGAGATCTAT TTTGTAAAGC ACTGAAAACA
    TACAATATGC TTTGCTTTGG AATTTATCGA TTAAGGGACG CTCATCTAAG TACTCCCAGC
    CAATGCACTA AACGTTATGT TATCACAAAC CCACCATATG AGTTTGAGCT TGTGCCGACA
    GACTTGATCT TCTGTTTGAT GCAATTCGAC CACAATGCTG GCCAGTCCCG GGCCAGTCTT
    TCTCATTCCT CCCATTCCTC CTATTCTTCC AGCAAGAAGA GCTCATCAGT TCACTCCATC
    CCATCAACAG CAAACCGACC GAACCGCACC AAGACCAGGG ATTCCCGGGA AAAACAGAAT
    GCAACAAGGA TGAATAGAAT GGGCCAAGCA GAAAAGAAAT GGTTTACAGA TGAACCGGAT
    AATGCCTATC CCAGAAACAT TCAAATCAAG CCCATGAGCA CCCACATGGC CAATCAGATC
    AATCAATACA AATCGACAAG CAGCTTGATA CCACCAATCA GAGAAGTTGA AGATGAATGT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37433
    Clone ID Related Accession (Same CDS sequence) XM_015288160.1 , XM_015288160.2
    Accession Version XM_015288160.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3723bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288160.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGCTAAG CCTGGCAAAC TTCTGCCTTT GGTTTCAATC
    AGTCAGGAAA AAAACAGTGG AACCCACATT CTAATGATAA CTGAACTGGT AAATGATTCA
    AATGTTCAGT TCTTGGACCA AGATGATGAT GATGATCCAG ATACAGAACT GTACCTCACG
    CAGCCCTTTG CATGTGGAAC CGCATTTGCT GTCAGCGTGC TGGACTCTCT CATGAGCGCA
    ACATACTTCA ATGACAATAT CCTCACACTG ATACGGACAT TGGTAACAGG AGGAGCCACA
    CCAGAGCTGG AAGCACTGAT TGCCGAGGAA AATGCTTTGA GAGGAGGTTA CAGCACGCCC
    CAGACACTTG CAAACAGGGA CAGGTGTCGA GTTGCTCAGT TGGCTTTGTA TGATGGGCCA
    TTTGCAGACC TAGGGGATGG AGGCTGCTAT GGAGATCTAT TTTGTAAAGC ACTGAAAACA
    TACAATATGC TTTGCTTTGG AATTTATCGA TTAAGGGACG CTCATCTAAG TACTCCCAGC
    CAATGCACTA AACGTTATGT TATCACAAAC CCACCATATG AGTTTGAGCT TGTGCCGACA
    GACTTGATCT TCTGTTTGAT GCAATTCGAC CACAATGCTG GCCAGTCCCG GGCCAGTCTT
    TCTCATTCCT CCCATTCCTC CTATTCTTCC AGCAAGAAGA GCTCATCAGT TCACTCCATC
    CCATCAACAG CAAACCGACC GAACCGCACC AAGACCAGGG ATTCCCGGGA AAAACAGAAT
    GCAACAAGGA TGAATAGAAT GGGCCAAGCA GAAAAGAAAT GGTTTACAGA TGAACCGGAT
    AATGCCTATC CCAGAAACAT TCAAATCAAG CCCATGAGCA CCCACATGGC CAATCAGATC
    AATCAATACA AATCGACAAG CAGCTTGATA CCACCAATCA GAGAAGTTGA AGATGAATGT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143646.1
    CDS368..4090
    Translation

    Target ORF information:

    RefSeq Version XM_015288160.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288160.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGCTAAG CCTGGCAAAC TTCTGCCTTT GGTTTCAATC
    AGTCAGGAAA AAAACAGTGG AACCCACATT CTAATGATAA CTGAACTGGT AAATGATTCA
    AATGTTCAGT TCTTGGACCA AGATGATGAT GATGATCCAG ATACAGAACT GTACCTCACG
    CAGCCCTTTG CATGTGGAAC CGCATTTGCT GTCAGCGTGC TGGACTCTCT CATGAGCGCA
    ACATACTTCA ATGACAATAT CCTCACACTG ATACGGACAT TGGTAACAGG AGGAGCCACA
    CCAGAGCTGG AAGCACTGAT TGCCGAGGAA AATGCTTTGA GAGGAGGTTA CAGCACGCCC
    CAGACACTTG CAAACAGGGA CAGGTGTCGA GTTGCTCAGT TGGCTTTGTA TGATGGGCCA
    TTTGCAGACC TAGGGGATGG AGGCTGCTAT GGAGATCTAT TTTGTAAAGC ACTGAAAACA
    TACAATATGC TTTGCTTTGG AATTTATCGA TTAAGGGACG CTCATCTAAG TACTCCCAGC
    CAATGCACTA AACGTTATGT TATCACAAAC CCACCATATG AGTTTGAGCT TGTGCCGACA
    GACTTGATCT TCTGTTTGAT GCAATTCGAC CACAATGCTG GCCAGTCCCG GGCCAGTCTT
    TCTCATTCCT CCCATTCCTC CTATTCTTCC AGCAAGAAGA GCTCATCAGT TCACTCCATC
    CCATCAACAG CAAACCGACC GAACCGCACC AAGACCAGGG ATTCCCGGGA AAAACAGAAT
    GCAACAAGGA TGAATAGAAT GGGCCAAGCA GAAAAGAAAT GGTTTACAGA TGAACCGGAT
    AATGCCTATC CCAGAAACAT TCAAATCAAG CCCATGAGCA CCCACATGGC CAATCAGATC
    AATCAATACA AATCGACAAG CAGCTTGATA CCACCAATCA GAGAAGTTGA AGATGAATGT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37434
    Clone ID Related Accession (Same CDS sequence) XM_015288161.1 , XM_015288161.2
    Accession Version XM_015288161.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGCTAAG CCTGGCAAAC TTCTGCCTTT GGTTTCAATC
    AGTCAGGAAA AAAACAGTGG AACCCACATT CTAATGATAA CTGAACTGGT AAATGATTCA
    AATGTTCAGT TCTTGGACCA AGATGATGAT GATGATCCAG ATACAGAACT GTACCTCACG
    CAGCCCTTTG CATGTGGAAC CGCATTTGCT GTCAGCGTGC TGGACTCTCT CATGAGCGCA
    ACATACTTCA ATGACAATAT CCTCACACTG ATACGGACAT TGGTAACAGG AGGAGCCACA
    CCAGAGCTGG AAGCACTGAT TGCCGAGGAA AATGCTTTGA GAGGAGGTTA CAGCACGCCC
    CAGACACTTG CAAACAGGGA CAGGTGTCGA GTTGCTCAGT TGGCTTTGTA TGATGGGCCA
    TTTGCAGACC TAGGGGATGG AGGCTGCTAT GGAGATCTAT TTTGTAAAGC ACTGAAAACA
    TACAATATGC TTTGCTTTGG AATTTATCGA TTAAGGGACG CTCATCTAAG TACTCCCAGC
    CAATGCACTA AACGTTATGT TATCACAAAC CCACCATATG AGTTTGAGCT TGTGCCGACA
    GACTTGATCT TCTGTTTGAT GCAATTCGAC CACAATGCTG GCCAGTCCCG GGCCAGTCTT
    TCTCATTCCT CCCATTCCTC CTATTCTTCC AGCAAGAAGA GCTCATCAGT TCACTCCATC
    CCATCAACAG CAAACCGACC GAACCGCACC AAGACCAGGG ATTCCCGGGA AAAACAGAAT
    GCAACAAGGA TGAATAGAAT GGGCCAAGAA AAGAAATGGT TTACAGATGA ACCGGATAAT
    GCCTATCCCA GAAACATTCA AATCAAGCCC ATGAGCACCC ACATGGCCAA TCAGATCAAT
    CAATACAAAT CGACAAGCAG CTTGATACCA CCAATCAGAG AAGTTGAAGA TGAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143647.1
    CDS373..4092
    Translation

    Target ORF information:

    RefSeq Version XM_015288161.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288161.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGCTAAG CCTGGCAAAC TTCTGCCTTT GGTTTCAATC
    AGTCAGGAAA AAAACAGTGG AACCCACATT CTAATGATAA CTGAACTGGT AAATGATTCA
    AATGTTCAGT TCTTGGACCA AGATGATGAT GATGATCCAG ATACAGAACT GTACCTCACG
    CAGCCCTTTG CATGTGGAAC CGCATTTGCT GTCAGCGTGC TGGACTCTCT CATGAGCGCA
    ACATACTTCA ATGACAATAT CCTCACACTG ATACGGACAT TGGTAACAGG AGGAGCCACA
    CCAGAGCTGG AAGCACTGAT TGCCGAGGAA AATGCTTTGA GAGGAGGTTA CAGCACGCCC
    CAGACACTTG CAAACAGGGA CAGGTGTCGA GTTGCTCAGT TGGCTTTGTA TGATGGGCCA
    TTTGCAGACC TAGGGGATGG AGGCTGCTAT GGAGATCTAT TTTGTAAAGC ACTGAAAACA
    TACAATATGC TTTGCTTTGG AATTTATCGA TTAAGGGACG CTCATCTAAG TACTCCCAGC
    CAATGCACTA AACGTTATGT TATCACAAAC CCACCATATG AGTTTGAGCT TGTGCCGACA
    GACTTGATCT TCTGTTTGAT GCAATTCGAC CACAATGCTG GCCAGTCCCG GGCCAGTCTT
    TCTCATTCCT CCCATTCCTC CTATTCTTCC AGCAAGAAGA GCTCATCAGT TCACTCCATC
    CCATCAACAG CAAACCGACC GAACCGCACC AAGACCAGGG ATTCCCGGGA AAAACAGAAT
    GCAACAAGGA TGAATAGAAT GGGCCAAGAA AAGAAATGGT TTACAGATGA ACCGGATAAT
    GCCTATCCCA GAAACATTCA AATCAAGCCC ATGAGCACCC ACATGGCCAA TCAGATCAAT
    CAATACAAAT CGACAAGCAG CTTGATACCA CCAATCAGAG AAGTTGAAGA TGAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37434
    Clone ID Related Accession (Same CDS sequence) XM_015288161.1 , XM_015288161.2
    Accession Version XM_015288161.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288161.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGCTAAG CCTGGCAAAC TTCTGCCTTT GGTTTCAATC
    AGTCAGGAAA AAAACAGTGG AACCCACATT CTAATGATAA CTGAACTGGT AAATGATTCA
    AATGTTCAGT TCTTGGACCA AGATGATGAT GATGATCCAG ATACAGAACT GTACCTCACG
    CAGCCCTTTG CATGTGGAAC CGCATTTGCT GTCAGCGTGC TGGACTCTCT CATGAGCGCA
    ACATACTTCA ATGACAATAT CCTCACACTG ATACGGACAT TGGTAACAGG AGGAGCCACA
    CCAGAGCTGG AAGCACTGAT TGCCGAGGAA AATGCTTTGA GAGGAGGTTA CAGCACGCCC
    CAGACACTTG CAAACAGGGA CAGGTGTCGA GTTGCTCAGT TGGCTTTGTA TGATGGGCCA
    TTTGCAGACC TAGGGGATGG AGGCTGCTAT GGAGATCTAT TTTGTAAAGC ACTGAAAACA
    TACAATATGC TTTGCTTTGG AATTTATCGA TTAAGGGACG CTCATCTAAG TACTCCCAGC
    CAATGCACTA AACGTTATGT TATCACAAAC CCACCATATG AGTTTGAGCT TGTGCCGACA
    GACTTGATCT TCTGTTTGAT GCAATTCGAC CACAATGCTG GCCAGTCCCG GGCCAGTCTT
    TCTCATTCCT CCCATTCCTC CTATTCTTCC AGCAAGAAGA GCTCATCAGT TCACTCCATC
    CCATCAACAG CAAACCGACC GAACCGCACC AAGACCAGGG ATTCCCGGGA AAAACAGAAT
    GCAACAAGGA TGAATAGAAT GGGCCAAGAA AAGAAATGGT TTACAGATGA ACCGGATAAT
    GCCTATCCCA GAAACATTCA AATCAAGCCC ATGAGCACCC ACATGGCCAA TCAGATCAAT
    CAATACAAAT CGACAAGCAG CTTGATACCA CCAATCAGAG AAGTTGAAGA TGAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143647.1
    CDS368..4087
    Translation

    Target ORF information:

    RefSeq Version XM_015288161.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288161.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGCTAAG CCTGGCAAAC TTCTGCCTTT GGTTTCAATC
    AGTCAGGAAA AAAACAGTGG AACCCACATT CTAATGATAA CTGAACTGGT AAATGATTCA
    AATGTTCAGT TCTTGGACCA AGATGATGAT GATGATCCAG ATACAGAACT GTACCTCACG
    CAGCCCTTTG CATGTGGAAC CGCATTTGCT GTCAGCGTGC TGGACTCTCT CATGAGCGCA
    ACATACTTCA ATGACAATAT CCTCACACTG ATACGGACAT TGGTAACAGG AGGAGCCACA
    CCAGAGCTGG AAGCACTGAT TGCCGAGGAA AATGCTTTGA GAGGAGGTTA CAGCACGCCC
    CAGACACTTG CAAACAGGGA CAGGTGTCGA GTTGCTCAGT TGGCTTTGTA TGATGGGCCA
    TTTGCAGACC TAGGGGATGG AGGCTGCTAT GGAGATCTAT TTTGTAAAGC ACTGAAAACA
    TACAATATGC TTTGCTTTGG AATTTATCGA TTAAGGGACG CTCATCTAAG TACTCCCAGC
    CAATGCACTA AACGTTATGT TATCACAAAC CCACCATATG AGTTTGAGCT TGTGCCGACA
    GACTTGATCT TCTGTTTGAT GCAATTCGAC CACAATGCTG GCCAGTCCCG GGCCAGTCTT
    TCTCATTCCT CCCATTCCTC CTATTCTTCC AGCAAGAAGA GCTCATCAGT TCACTCCATC
    CCATCAACAG CAAACCGACC GAACCGCACC AAGACCAGGG ATTCCCGGGA AAAACAGAAT
    GCAACAAGGA TGAATAGAAT GGGCCAAGAA AAGAAATGGT TTACAGATGA ACCGGATAAT
    GCCTATCCCA GAAACATTCA AATCAAGCCC ATGAGCACCC ACATGGCCAA TCAGATCAAT
    CAATACAAAT CGACAAGCAG CTTGATACCA CCAATCAGAG AAGTTGAAGA TGAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37435
    Clone ID Related Accession (Same CDS sequence) XM_015288162.1 , XM_015288162.2
    Accession Version XM_015288162.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGCTAA GCCTGGCAAA CTTCTGCCTT TGGTTTCAAT CAGTCAGGAA
    AAAAACAGTG GAACCCACAT TCTAATGATA ACTGAACTGG TAAATGATTC AAATGTTCAG
    TTCTTGGACC AAGATGATGA TGATGATCCA GATACAGAAC TGTACCTCAC GCAGCCCTTT
    GCATGTGGAA CCGCATTTGC TGTCAGCGTG CTGGACTCTC TCATGAGCGC AACATACTTC
    AATGACAATA TCCTCACACT GATACGGACA TTGGTAACAG GAGGAGCCAC ACCAGAGCTG
    GAAGCACTGA TTGCCGAGGA AAATGCTTTG AGAGGAGGTT ACAGCACGCC CCAGACACTT
    GCAAACAGGG ACAGGTGTCG AGTTGCTCAG TTGGCTTTGT ATGATGGGCC ATTTGCAGAC
    CTAGGGGATG GAGGCTGCTA TGGAGATCTA TTTTGTAAAG CACTGAAAAC ATACAATATG
    CTTTGCTTTG GAATTTATCG ATTAAGGGAC GCTCATCTAA GTACTCCCAG CCAATGCACT
    AAACGTTATG TTATCACAAA CCCACCATAT GAGTTTGAGC TTGTGCCGAC AGACTTGATC
    TTCTGTTTGA TGCAATTCGA CCACAATGCT GGCCAGTCCC GGGCCAGTCT TTCTCATTCC
    TCCCATTCCT CCTATTCTTC CAGCAAGAAG AGCTCATCAG TTCACTCCAT CCCATCAACA
    GCAAACCGAC CGAACCGCAC CAAGACCAGG GATTCCCGGG AAAAACAGAA TGCAACAAGG
    ATGAATAGAA TGGGCCAAGC AGAAAAGAAA TGGTTTACAG ATGAACCGGA TAATGCCTAT
    CCCAGAAACA TTCAAATCAA GCCCATGAGC ACCCACATGG CCAATCAGAT CAATCAATAC
    AAATCGACAA GCAGCTTGAT ACCACCAATC AGAGAAGTTG AAGATGAATG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143648.1
    CDS373..4086
    Translation

    Target ORF information:

    RefSeq Version XM_015288162.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288162.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGCTAA GCCTGGCAAA CTTCTGCCTT TGGTTTCAAT CAGTCAGGAA
    AAAAACAGTG GAACCCACAT TCTAATGATA ACTGAACTGG TAAATGATTC AAATGTTCAG
    TTCTTGGACC AAGATGATGA TGATGATCCA GATACAGAAC TGTACCTCAC GCAGCCCTTT
    GCATGTGGAA CCGCATTTGC TGTCAGCGTG CTGGACTCTC TCATGAGCGC AACATACTTC
    AATGACAATA TCCTCACACT GATACGGACA TTGGTAACAG GAGGAGCCAC ACCAGAGCTG
    GAAGCACTGA TTGCCGAGGA AAATGCTTTG AGAGGAGGTT ACAGCACGCC CCAGACACTT
    GCAAACAGGG ACAGGTGTCG AGTTGCTCAG TTGGCTTTGT ATGATGGGCC ATTTGCAGAC
    CTAGGGGATG GAGGCTGCTA TGGAGATCTA TTTTGTAAAG CACTGAAAAC ATACAATATG
    CTTTGCTTTG GAATTTATCG ATTAAGGGAC GCTCATCTAA GTACTCCCAG CCAATGCACT
    AAACGTTATG TTATCACAAA CCCACCATAT GAGTTTGAGC TTGTGCCGAC AGACTTGATC
    TTCTGTTTGA TGCAATTCGA CCACAATGCT GGCCAGTCCC GGGCCAGTCT TTCTCATTCC
    TCCCATTCCT CCTATTCTTC CAGCAAGAAG AGCTCATCAG TTCACTCCAT CCCATCAACA
    GCAAACCGAC CGAACCGCAC CAAGACCAGG GATTCCCGGG AAAAACAGAA TGCAACAAGG
    ATGAATAGAA TGGGCCAAGC AGAAAAGAAA TGGTTTACAG ATGAACCGGA TAATGCCTAT
    CCCAGAAACA TTCAAATCAA GCCCATGAGC ACCCACATGG CCAATCAGAT CAATCAATAC
    AAATCGACAA GCAGCTTGAT ACCACCAATC AGAGAAGTTG AAGATGAATG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37435
    Clone ID Related Accession (Same CDS sequence) XM_015288162.1 , XM_015288162.2
    Accession Version XM_015288162.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288162.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGCTAA GCCTGGCAAA CTTCTGCCTT TGGTTTCAAT CAGTCAGGAA
    AAAAACAGTG GAACCCACAT TCTAATGATA ACTGAACTGG TAAATGATTC AAATGTTCAG
    TTCTTGGACC AAGATGATGA TGATGATCCA GATACAGAAC TGTACCTCAC GCAGCCCTTT
    GCATGTGGAA CCGCATTTGC TGTCAGCGTG CTGGACTCTC TCATGAGCGC AACATACTTC
    AATGACAATA TCCTCACACT GATACGGACA TTGGTAACAG GAGGAGCCAC ACCAGAGCTG
    GAAGCACTGA TTGCCGAGGA AAATGCTTTG AGAGGAGGTT ACAGCACGCC CCAGACACTT
    GCAAACAGGG ACAGGTGTCG AGTTGCTCAG TTGGCTTTGT ATGATGGGCC ATTTGCAGAC
    CTAGGGGATG GAGGCTGCTA TGGAGATCTA TTTTGTAAAG CACTGAAAAC ATACAATATG
    CTTTGCTTTG GAATTTATCG ATTAAGGGAC GCTCATCTAA GTACTCCCAG CCAATGCACT
    AAACGTTATG TTATCACAAA CCCACCATAT GAGTTTGAGC TTGTGCCGAC AGACTTGATC
    TTCTGTTTGA TGCAATTCGA CCACAATGCT GGCCAGTCCC GGGCCAGTCT TTCTCATTCC
    TCCCATTCCT CCTATTCTTC CAGCAAGAAG AGCTCATCAG TTCACTCCAT CCCATCAACA
    GCAAACCGAC CGAACCGCAC CAAGACCAGG GATTCCCGGG AAAAACAGAA TGCAACAAGG
    ATGAATAGAA TGGGCCAAGC AGAAAAGAAA TGGTTTACAG ATGAACCGGA TAATGCCTAT
    CCCAGAAACA TTCAAATCAA GCCCATGAGC ACCCACATGG CCAATCAGAT CAATCAATAC
    AAATCGACAA GCAGCTTGAT ACCACCAATC AGAGAAGTTG AAGATGAATG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143648.1
    CDS368..4081
    Translation

    Target ORF information:

    RefSeq Version XM_015288162.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288162.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGCTAA GCCTGGCAAA CTTCTGCCTT TGGTTTCAAT CAGTCAGGAA
    AAAAACAGTG GAACCCACAT TCTAATGATA ACTGAACTGG TAAATGATTC AAATGTTCAG
    TTCTTGGACC AAGATGATGA TGATGATCCA GATACAGAAC TGTACCTCAC GCAGCCCTTT
    GCATGTGGAA CCGCATTTGC TGTCAGCGTG CTGGACTCTC TCATGAGCGC AACATACTTC
    AATGACAATA TCCTCACACT GATACGGACA TTGGTAACAG GAGGAGCCAC ACCAGAGCTG
    GAAGCACTGA TTGCCGAGGA AAATGCTTTG AGAGGAGGTT ACAGCACGCC CCAGACACTT
    GCAAACAGGG ACAGGTGTCG AGTTGCTCAG TTGGCTTTGT ATGATGGGCC ATTTGCAGAC
    CTAGGGGATG GAGGCTGCTA TGGAGATCTA TTTTGTAAAG CACTGAAAAC ATACAATATG
    CTTTGCTTTG GAATTTATCG ATTAAGGGAC GCTCATCTAA GTACTCCCAG CCAATGCACT
    AAACGTTATG TTATCACAAA CCCACCATAT GAGTTTGAGC TTGTGCCGAC AGACTTGATC
    TTCTGTTTGA TGCAATTCGA CCACAATGCT GGCCAGTCCC GGGCCAGTCT TTCTCATTCC
    TCCCATTCCT CCTATTCTTC CAGCAAGAAG AGCTCATCAG TTCACTCCAT CCCATCAACA
    GCAAACCGAC CGAACCGCAC CAAGACCAGG GATTCCCGGG AAAAACAGAA TGCAACAAGG
    ATGAATAGAA TGGGCCAAGC AGAAAAGAAA TGGTTTACAG ATGAACCGGA TAATGCCTAT
    CCCAGAAACA TTCAAATCAA GCCCATGAGC ACCCACATGG CCAATCAGAT CAATCAATAC
    AAATCGACAA GCAGCTTGAT ACCACCAATC AGAGAAGTTG AAGATGAATG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37436
    Clone ID Related Accession (Same CDS sequence) XM_015288163.2 , XM_015288163.1
    Accession Version XM_015288163.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288163.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGCTAA GCCTGGCAAA CTTCTGCCTT TGGTTTCAAT CAGTCAGGAA
    AAAAACAGTG GAACCCACAT TCTAATGATA ACTGAACTGG TAAATGATTC AAATGTTCAG
    TTCTTGGACC AAGATGATGA TGATGATCCA GATACAGAAC TGTACCTCAC GCAGCCCTTT
    GCATGTGGAA CCGCATTTGC TGTCAGCGTG CTGGACTCTC TCATGAGCGC AACATACTTC
    AATGACAATA TCCTCACACT GATACGGACA TTGGTAACAG GAGGAGCCAC ACCAGAGCTG
    GAAGCACTGA TTGCCGAGGA AAATGCTTTG AGAGGAGGTT ACAGCACGCC CCAGACACTT
    GCAAACAGGG ACAGGTGTCG AGTTGCTCAG TTGGCTTTGT ATGATGGGCC ATTTGCAGAC
    CTAGGGGATG GAGGCTGCTA TGGAGATCTA TTTTGTAAAG CACTGAAAAC ATACAATATG
    CTTTGCTTTG GAATTTATCG ATTAAGGGAC GCTCATCTAA GTACTCCCAG CCAATGCACT
    AAACGTTATG TTATCACAAA CCCACCATAT GAGTTTGAGC TTGTGCCGAC AGACTTGATC
    TTCTGTTTGA TGCAATTCGA CCACAATGCT GGCCAGTCCC GGGCCAGTCT TTCTCATTCC
    TCCCATTCCT CCTATTCTTC CAGCAAGAAG AGCTCATCAG TTCACTCCAT CCCATCAACA
    GCAAACCGAC CGAACCGCAC CAAGACCAGG GATTCCCGGG AAAAACAGAA TGCAACAAGG
    ATGAATAGAA TGGGCCAAGA AAAGAAATGG TTTACAGATG AACCGGATAA TGCCTATCCC
    AGAAACATTC AAATCAAGCC CATGAGCACC CACATGGCCA ATCAGATCAA TCAATACAAA
    TCGACAAGCA GCTTGATACC ACCAATCAGA GAAGTTGAAG ATGAATGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143649.1
    CDS368..4078
    Translation

    Target ORF information:

    RefSeq Version XM_015288163.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288163.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGCTAA GCCTGGCAAA CTTCTGCCTT TGGTTTCAAT CAGTCAGGAA
    AAAAACAGTG GAACCCACAT TCTAATGATA ACTGAACTGG TAAATGATTC AAATGTTCAG
    TTCTTGGACC AAGATGATGA TGATGATCCA GATACAGAAC TGTACCTCAC GCAGCCCTTT
    GCATGTGGAA CCGCATTTGC TGTCAGCGTG CTGGACTCTC TCATGAGCGC AACATACTTC
    AATGACAATA TCCTCACACT GATACGGACA TTGGTAACAG GAGGAGCCAC ACCAGAGCTG
    GAAGCACTGA TTGCCGAGGA AAATGCTTTG AGAGGAGGTT ACAGCACGCC CCAGACACTT
    GCAAACAGGG ACAGGTGTCG AGTTGCTCAG TTGGCTTTGT ATGATGGGCC ATTTGCAGAC
    CTAGGGGATG GAGGCTGCTA TGGAGATCTA TTTTGTAAAG CACTGAAAAC ATACAATATG
    CTTTGCTTTG GAATTTATCG ATTAAGGGAC GCTCATCTAA GTACTCCCAG CCAATGCACT
    AAACGTTATG TTATCACAAA CCCACCATAT GAGTTTGAGC TTGTGCCGAC AGACTTGATC
    TTCTGTTTGA TGCAATTCGA CCACAATGCT GGCCAGTCCC GGGCCAGTCT TTCTCATTCC
    TCCCATTCCT CCTATTCTTC CAGCAAGAAG AGCTCATCAG TTCACTCCAT CCCATCAACA
    GCAAACCGAC CGAACCGCAC CAAGACCAGG GATTCCCGGG AAAAACAGAA TGCAACAAGG
    ATGAATAGAA TGGGCCAAGA AAAGAAATGG TTTACAGATG AACCGGATAA TGCCTATCCC
    AGAAACATTC AAATCAAGCC CATGAGCACC CACATGGCCA ATCAGATCAA TCAATACAAA
    TCGACAAGCA GCTTGATACC ACCAATCAGA GAAGTTGAAG ATGAATGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37436
    Clone ID Related Accession (Same CDS sequence) XM_015288163.2 , XM_015288163.1
    Accession Version XM_015288163.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGCTAA GCCTGGCAAA CTTCTGCCTT TGGTTTCAAT CAGTCAGGAA
    AAAAACAGTG GAACCCACAT TCTAATGATA ACTGAACTGG TAAATGATTC AAATGTTCAG
    TTCTTGGACC AAGATGATGA TGATGATCCA GATACAGAAC TGTACCTCAC GCAGCCCTTT
    GCATGTGGAA CCGCATTTGC TGTCAGCGTG CTGGACTCTC TCATGAGCGC AACATACTTC
    AATGACAATA TCCTCACACT GATACGGACA TTGGTAACAG GAGGAGCCAC ACCAGAGCTG
    GAAGCACTGA TTGCCGAGGA AAATGCTTTG AGAGGAGGTT ACAGCACGCC CCAGACACTT
    GCAAACAGGG ACAGGTGTCG AGTTGCTCAG TTGGCTTTGT ATGATGGGCC ATTTGCAGAC
    CTAGGGGATG GAGGCTGCTA TGGAGATCTA TTTTGTAAAG CACTGAAAAC ATACAATATG
    CTTTGCTTTG GAATTTATCG ATTAAGGGAC GCTCATCTAA GTACTCCCAG CCAATGCACT
    AAACGTTATG TTATCACAAA CCCACCATAT GAGTTTGAGC TTGTGCCGAC AGACTTGATC
    TTCTGTTTGA TGCAATTCGA CCACAATGCT GGCCAGTCCC GGGCCAGTCT TTCTCATTCC
    TCCCATTCCT CCTATTCTTC CAGCAAGAAG AGCTCATCAG TTCACTCCAT CCCATCAACA
    GCAAACCGAC CGAACCGCAC CAAGACCAGG GATTCCCGGG AAAAACAGAA TGCAACAAGG
    ATGAATAGAA TGGGCCAAGA AAAGAAATGG TTTACAGATG AACCGGATAA TGCCTATCCC
    AGAAACATTC AAATCAAGCC CATGAGCACC CACATGGCCA ATCAGATCAA TCAATACAAA
    TCGACAAGCA GCTTGATACC ACCAATCAGA GAAGTTGAAG ATGAATGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143649.1
    CDS373..4083
    Translation

    Target ORF information:

    RefSeq Version XM_015288163.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288163.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGCTAA GCCTGGCAAA CTTCTGCCTT TGGTTTCAAT CAGTCAGGAA
    AAAAACAGTG GAACCCACAT TCTAATGATA ACTGAACTGG TAAATGATTC AAATGTTCAG
    TTCTTGGACC AAGATGATGA TGATGATCCA GATACAGAAC TGTACCTCAC GCAGCCCTTT
    GCATGTGGAA CCGCATTTGC TGTCAGCGTG CTGGACTCTC TCATGAGCGC AACATACTTC
    AATGACAATA TCCTCACACT GATACGGACA TTGGTAACAG GAGGAGCCAC ACCAGAGCTG
    GAAGCACTGA TTGCCGAGGA AAATGCTTTG AGAGGAGGTT ACAGCACGCC CCAGACACTT
    GCAAACAGGG ACAGGTGTCG AGTTGCTCAG TTGGCTTTGT ATGATGGGCC ATTTGCAGAC
    CTAGGGGATG GAGGCTGCTA TGGAGATCTA TTTTGTAAAG CACTGAAAAC ATACAATATG
    CTTTGCTTTG GAATTTATCG ATTAAGGGAC GCTCATCTAA GTACTCCCAG CCAATGCACT
    AAACGTTATG TTATCACAAA CCCACCATAT GAGTTTGAGC TTGTGCCGAC AGACTTGATC
    TTCTGTTTGA TGCAATTCGA CCACAATGCT GGCCAGTCCC GGGCCAGTCT TTCTCATTCC
    TCCCATTCCT CCTATTCTTC CAGCAAGAAG AGCTCATCAG TTCACTCCAT CCCATCAACA
    GCAAACCGAC CGAACCGCAC CAAGACCAGG GATTCCCGGG AAAAACAGAA TGCAACAAGG
    ATGAATAGAA TGGGCCAAGA AAAGAAATGG TTTACAGATG AACCGGATAA TGCCTATCCC
    AGAAACATTC AAATCAAGCC CATGAGCACC CACATGGCCA ATCAGATCAA TCAATACAAA
    TCGACAAGCA GCTTGATACC ACCAATCAGA GAAGTTGAAG ATGAATGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37437
    Clone ID Related Accession (Same CDS sequence) XM_015288164.2 , XM_015288164.1
    Accession Version XM_015288164.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3666bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288164.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GTAAATGATT CAAATGTTCA GTTCTTGGAC
    CAAGATGATG ATGATGATCC AGATACAGAA CTGTACCTCA CGCAGCCCTT TGCATGTGGA
    ACCGCATTTG CTGTCAGCGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT
    ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG
    ATTGCCGAGG AAAATGCTTT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG
    GACAGGTGTC GAGTTGCTCA GTTGGCTTTG TATGATGGGC CATTTGCAGA CCTAGGGGAT
    GGAGGCTGCT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT
    GGAATTTATC GATTAAGGGA CGCTCATCTA AGTACTCCCA GCCAATGCAC TAAACGTTAT
    GTTATCACAA ACCCACCATA TGAGTTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG
    ATGCAATTCG ACCACAATGC TGGCCAGTCC CGGGCCAGTC TTTCTCATTC CTCCCATTCC
    TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCCA TCCCATCAAC AGCAAACCGA
    CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA ACAGAAAAGA AATGGTTTAC
    AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143650.1
    CDS368..4033
    Translation

    Target ORF information:

    RefSeq Version XM_015288164.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288164.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GTAAATGATT CAAATGTTCA GTTCTTGGAC
    CAAGATGATG ATGATGATCC AGATACAGAA CTGTACCTCA CGCAGCCCTT TGCATGTGGA
    ACCGCATTTG CTGTCAGCGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT
    ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG
    ATTGCCGAGG AAAATGCTTT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG
    GACAGGTGTC GAGTTGCTCA GTTGGCTTTG TATGATGGGC CATTTGCAGA CCTAGGGGAT
    GGAGGCTGCT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT
    GGAATTTATC GATTAAGGGA CGCTCATCTA AGTACTCCCA GCCAATGCAC TAAACGTTAT
    GTTATCACAA ACCCACCATA TGAGTTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG
    ATGCAATTCG ACCACAATGC TGGCCAGTCC CGGGCCAGTC TTTCTCATTC CTCCCATTCC
    TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCCA TCCCATCAAC AGCAAACCGA
    CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA ACAGAAAAGA AATGGTTTAC
    AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37437
    Clone ID Related Accession (Same CDS sequence) XM_015288164.2 , XM_015288164.1
    Accession Version XM_015288164.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3666bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GTAAATGATT CAAATGTTCA GTTCTTGGAC
    CAAGATGATG ATGATGATCC AGATACAGAA CTGTACCTCA CGCAGCCCTT TGCATGTGGA
    ACCGCATTTG CTGTCAGCGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT
    ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG
    ATTGCCGAGG AAAATGCTTT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG
    GACAGGTGTC GAGTTGCTCA GTTGGCTTTG TATGATGGGC CATTTGCAGA CCTAGGGGAT
    GGAGGCTGCT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT
    GGAATTTATC GATTAAGGGA CGCTCATCTA AGTACTCCCA GCCAATGCAC TAAACGTTAT
    GTTATCACAA ACCCACCATA TGAGTTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG
    ATGCAATTCG ACCACAATGC TGGCCAGTCC CGGGCCAGTC TTTCTCATTC CTCCCATTCC
    TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCCA TCCCATCAAC AGCAAACCGA
    CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA ACAGAAAAGA AATGGTTTAC
    AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143650.1
    CDS373..4038
    Translation

    Target ORF information:

    RefSeq Version XM_015288164.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288164.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTCCA AGATGTCCAT CTACAAGAGA ATGAAACTGG CATGTTGTTT TGATTGCGGA
    CGCTCTGAGC GTGACTGCTC TTGCATGTCA GGCAGTGTAC ATAGTAACAT GGACACCCTT
    GAGAGAGCCT TCCCACTTTC TTCTGTCTCT GTTAATGATT GCTCCACCAG TTTACGTGCC
    TTTGAAGATG AGCAGCCGTC AACACTATCA CCCAAAAAAA AGCAACGAAA TGGAGGCATG
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC
    AATGAACAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC GGGAATGTTT
    CATTGGTGTC CAGCCAAGGA TATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC CCTGCATAAC
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGTATGGAT
    AGGTCATCTC CAGACAACAG TCCTGTCCAC GGCCTGTTAC GTCAACCCTC CATTACAACA
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GTAAATGATT CAAATGTTCA GTTCTTGGAC
    CAAGATGATG ATGATGATCC AGATACAGAA CTGTACCTCA CGCAGCCCTT TGCATGTGGA
    ACCGCATTTG CTGTCAGCGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT
    ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG
    ATTGCCGAGG AAAATGCTTT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG
    GACAGGTGTC GAGTTGCTCA GTTGGCTTTG TATGATGGGC CATTTGCAGA CCTAGGGGAT
    GGAGGCTGCT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT
    GGAATTTATC GATTAAGGGA CGCTCATCTA AGTACTCCCA GCCAATGCAC TAAACGTTAT
    GTTATCACAA ACCCACCATA TGAGTTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG
    ATGCAATTCG ACCACAATGC TGGCCAGTCC CGGGCCAGTC TTTCTCATTC CTCCCATTCC
    TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCCA TCCCATCAAC AGCAAACCGA
    CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA ACAGAAAAGA AATGGTTTAC
    AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37438
    Clone ID Related Accession (Same CDS sequence) XM_015288165.1 , XM_015288165.2
    Accession Version XM_015288165.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3657bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCCC
    AAGATGTCCA TCTACAAGAG AATGAAACTG GCATGTTGTT TTGATTGCGG ACGCTCTGAG
    CGTGACTGCT CTTGCATGTC AGGCAGTGTA CATAGTAACA TGGACACCCT TGAGAGAGCC
    TTCCCACTTT CTTCTGTCTC TGTTAATGAT TGCTCCACCA GTTTACGTGC CTTTGAAGAT
    GAGCAGCCGT CAACACTATC ACCCAAAAAA AAGCAACGAA ATGGAGGCAT GCGAAATTCA
    CCCAACTCCT CACCCAAACT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAATGAACAG
    ATTGACAATA TGGATGCCAA TGTGAAAAAA TATGACTCTA CGGGAATGTT TCATTGGTGT
    CCAGCCAAGG ATATTGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA
    AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA
    AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG
    TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CCCTGCATAA CTTCCCCAAG
    GTTTCAATCT TGCCTGGTAC ACCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC
    CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG
    CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC
    ATTGGGGTCT TGCAGGCTAA TTCCCAAGGC TTTACACCCC CTGGTATGGA TAGGTCATCT
    CCAGACAACA GTCCTGTCCA CGGCCTGTTA CGTCAACCCT CCATTACAAC AGGAGCCAAC
    ATCCCTATTA TAACAGAGCT AGTAAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT
    GATGATGATC CAGATACAGA ACTGTACCTC ACGCAGCCCT TTGCATGTGG AACCGCATTT
    GCTGTCAGCG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA
    CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCCGAG
    GAAAATGCTT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT
    CGAGTTGCTC AGTTGGCTTT GTATGATGGG CCATTTGCAG ACCTAGGGGA TGGAGGCTGC
    TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT
    CGATTAAGGG ACGCTCATCT AAGTACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA
    AACCCACCAT ATGAGTTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTC
    GACCACAATG CTGGCCAGTC CCGGGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT
    TCCAGCAAGA AGAGCTCATC AGTTCACTCC ATCCCATCAA CAGCAAACCG ACCGAACCGC
    ACCAAGACCA GGGATTCCCG GGAAAAACAG AACAGAAAAG AAATGGTTTA CAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143651.1
    CDS373..4029
    Translation

    Target ORF information:

    RefSeq Version XM_015288165.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288165.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCCC
    AAGATGTCCA TCTACAAGAG AATGAAACTG GCATGTTGTT TTGATTGCGG ACGCTCTGAG
    CGTGACTGCT CTTGCATGTC AGGCAGTGTA CATAGTAACA TGGACACCCT TGAGAGAGCC
    TTCCCACTTT CTTCTGTCTC TGTTAATGAT TGCTCCACCA GTTTACGTGC CTTTGAAGAT
    GAGCAGCCGT CAACACTATC ACCCAAAAAA AAGCAACGAA ATGGAGGCAT GCGAAATTCA
    CCCAACTCCT CACCCAAACT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAATGAACAG
    ATTGACAATA TGGATGCCAA TGTGAAAAAA TATGACTCTA CGGGAATGTT TCATTGGTGT
    CCAGCCAAGG ATATTGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA
    AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA
    AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG
    TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CCCTGCATAA CTTCCCCAAG
    GTTTCAATCT TGCCTGGTAC ACCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC
    CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG
    CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC
    ATTGGGGTCT TGCAGGCTAA TTCCCAAGGC TTTACACCCC CTGGTATGGA TAGGTCATCT
    CCAGACAACA GTCCTGTCCA CGGCCTGTTA CGTCAACCCT CCATTACAAC AGGAGCCAAC
    ATCCCTATTA TAACAGAGCT AGTAAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT
    GATGATGATC CAGATACAGA ACTGTACCTC ACGCAGCCCT TTGCATGTGG AACCGCATTT
    GCTGTCAGCG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA
    CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCCGAG
    GAAAATGCTT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT
    CGAGTTGCTC AGTTGGCTTT GTATGATGGG CCATTTGCAG ACCTAGGGGA TGGAGGCTGC
    TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT
    CGATTAAGGG ACGCTCATCT AAGTACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA
    AACCCACCAT ATGAGTTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTC
    GACCACAATG CTGGCCAGTC CCGGGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT
    TCCAGCAAGA AGAGCTCATC AGTTCACTCC ATCCCATCAA CAGCAAACCG ACCGAACCGC
    ACCAAGACCA GGGATTCCCG GGAAAAACAG AACAGAAAAG AAATGGTTTA CAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37438
    Clone ID Related Accession (Same CDS sequence) XM_015288165.1 , XM_015288165.2
    Accession Version XM_015288165.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3657bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288165.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCCC
    AAGATGTCCA TCTACAAGAG AATGAAACTG GCATGTTGTT TTGATTGCGG ACGCTCTGAG
    CGTGACTGCT CTTGCATGTC AGGCAGTGTA CATAGTAACA TGGACACCCT TGAGAGAGCC
    TTCCCACTTT CTTCTGTCTC TGTTAATGAT TGCTCCACCA GTTTACGTGC CTTTGAAGAT
    GAGCAGCCGT CAACACTATC ACCCAAAAAA AAGCAACGAA ATGGAGGCAT GCGAAATTCA
    CCCAACTCCT CACCCAAACT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAATGAACAG
    ATTGACAATA TGGATGCCAA TGTGAAAAAA TATGACTCTA CGGGAATGTT TCATTGGTGT
    CCAGCCAAGG ATATTGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA
    AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA
    AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG
    TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CCCTGCATAA CTTCCCCAAG
    GTTTCAATCT TGCCTGGTAC ACCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC
    CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG
    CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC
    ATTGGGGTCT TGCAGGCTAA TTCCCAAGGC TTTACACCCC CTGGTATGGA TAGGTCATCT
    CCAGACAACA GTCCTGTCCA CGGCCTGTTA CGTCAACCCT CCATTACAAC AGGAGCCAAC
    ATCCCTATTA TAACAGAGCT AGTAAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT
    GATGATGATC CAGATACAGA ACTGTACCTC ACGCAGCCCT TTGCATGTGG AACCGCATTT
    GCTGTCAGCG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA
    CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCCGAG
    GAAAATGCTT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT
    CGAGTTGCTC AGTTGGCTTT GTATGATGGG CCATTTGCAG ACCTAGGGGA TGGAGGCTGC
    TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT
    CGATTAAGGG ACGCTCATCT AAGTACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA
    AACCCACCAT ATGAGTTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTC
    GACCACAATG CTGGCCAGTC CCGGGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT
    TCCAGCAAGA AGAGCTCATC AGTTCACTCC ATCCCATCAA CAGCAAACCG ACCGAACCGC
    ACCAAGACCA GGGATTCCCG GGAAAAACAG AACAGAAAAG AAATGGTTTA CAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143651.1
    CDS368..4024
    Translation

    Target ORF information:

    RefSeq Version XM_015288165.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288165.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCCC
    AAGATGTCCA TCTACAAGAG AATGAAACTG GCATGTTGTT TTGATTGCGG ACGCTCTGAG
    CGTGACTGCT CTTGCATGTC AGGCAGTGTA CATAGTAACA TGGACACCCT TGAGAGAGCC
    TTCCCACTTT CTTCTGTCTC TGTTAATGAT TGCTCCACCA GTTTACGTGC CTTTGAAGAT
    GAGCAGCCGT CAACACTATC ACCCAAAAAA AAGCAACGAA ATGGAGGCAT GCGAAATTCA
    CCCAACTCCT CACCCAAACT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAATGAACAG
    ATTGACAATA TGGATGCCAA TGTGAAAAAA TATGACTCTA CGGGAATGTT TCATTGGTGT
    CCAGCCAAGG ATATTGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA
    AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA
    AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG
    TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CCCTGCATAA CTTCCCCAAG
    GTTTCAATCT TGCCTGGTAC ACCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC
    CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG
    CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC
    ATTGGGGTCT TGCAGGCTAA TTCCCAAGGC TTTACACCCC CTGGTATGGA TAGGTCATCT
    CCAGACAACA GTCCTGTCCA CGGCCTGTTA CGTCAACCCT CCATTACAAC AGGAGCCAAC
    ATCCCTATTA TAACAGAGCT AGTAAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT
    GATGATGATC CAGATACAGA ACTGTACCTC ACGCAGCCCT TTGCATGTGG AACCGCATTT
    GCTGTCAGCG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA
    CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCCGAG
    GAAAATGCTT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT
    CGAGTTGCTC AGTTGGCTTT GTATGATGGG CCATTTGCAG ACCTAGGGGA TGGAGGCTGC
    TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT
    CGATTAAGGG ACGCTCATCT AAGTACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA
    AACCCACCAT ATGAGTTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTC
    GACCACAATG CTGGCCAGTC CCGGGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT
    TCCAGCAAGA AGAGCTCATC AGTTCACTCC ATCCCATCAA CAGCAAACCG ACCGAACCGC
    ACCAAGACCA GGGATTCCCG GGAAAAACAG AACAGAAAAG AAATGGTTTA CAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37439
    Clone ID Related Accession (Same CDS sequence) XM_015288166.2 , XM_015288166.1
    Accession Version XM_015288166.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3654bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288166.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCCC
    AAGATGTCCA TCTACAAGAG AATGAAACTG GCATGTTGTT TTGATTGCGG ACGCTCTGAG
    CGTGACTGCT CTTGCATGTC AGGCAGTGTA CATAGTAACA TGGACACCCT TGAGAGAGCC
    TTCCCACTTT CTTCTGTCTC TGTTAATGAT TGCTCCACCA GTTTACGTGC CTTTGAAGAT
    GAGCAGCCGT CAACACTATC ACCCAAAAAA AAGCAACGAA ATGGAGGCAT GCGAAATTCA
    CCCAACTCCT CACCCAAACT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAATGAACAG
    ATTGACAATA TGGATGCCAA TGTGAAAAAA TATGACTCTA CGGGAATGTT TCATTGGTGT
    CCAGCCAAGG ATATTGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA
    AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA
    AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG
    TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CCCTGCATAA CTTCCCCAAG
    GTTTCAATCT TGCCTGGTAC ACCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC
    CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG
    CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC
    ATTGGGGTCT TGCAGGCTAA TTCCCAAGGC TTTACACCCC CTGGTATGGA TAGGTCATCT
    CCAGACAACA GTCCTGTCCA CGGCCTGTTA CGTCAACCCT CCATTACAAC AGGAGCCAAC
    ATCCCTATTA TAACAGAGCT AGTAAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT
    GATGATGATC CAGATACAGA ACTGTACCTC ACGCAGCCCT TTGCATGTGG AACCGCATTT
    GCTGTCAGCG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA
    CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCCGAG
    GAAAATGCTT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT
    CGAGTTGCTC AGTTGGCTTT GTATGATGGG CCATTTGCAG ACCTAGGGGA TGGAGGCTGC
    TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT
    CGATTAAGGG ACGCTCATCT AAGTACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA
    AACCCACCAT ATGAGTTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTC
    GACCACAATG CTGGCCAGTC CCGGGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT
    TCCAGCAAGA AGAGCTCATC AGTTCACTCC ATCCCATCAA CAGCAAACCG ACCGAACCGC
    ACCAAGACCA GGGATTCCCG GGAAAAACAG AAAAAAGAAA TGGTTTACAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143652.1
    CDS368..4021
    Translation

    Target ORF information:

    RefSeq Version XM_015288166.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288166.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCCC
    AAGATGTCCA TCTACAAGAG AATGAAACTG GCATGTTGTT TTGATTGCGG ACGCTCTGAG
    CGTGACTGCT CTTGCATGTC AGGCAGTGTA CATAGTAACA TGGACACCCT TGAGAGAGCC
    TTCCCACTTT CTTCTGTCTC TGTTAATGAT TGCTCCACCA GTTTACGTGC CTTTGAAGAT
    GAGCAGCCGT CAACACTATC ACCCAAAAAA AAGCAACGAA ATGGAGGCAT GCGAAATTCA
    CCCAACTCCT CACCCAAACT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAATGAACAG
    ATTGACAATA TGGATGCCAA TGTGAAAAAA TATGACTCTA CGGGAATGTT TCATTGGTGT
    CCAGCCAAGG ATATTGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA
    AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA
    AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG
    TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CCCTGCATAA CTTCCCCAAG
    GTTTCAATCT TGCCTGGTAC ACCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC
    CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG
    CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC
    ATTGGGGTCT TGCAGGCTAA TTCCCAAGGC TTTACACCCC CTGGTATGGA TAGGTCATCT
    CCAGACAACA GTCCTGTCCA CGGCCTGTTA CGTCAACCCT CCATTACAAC AGGAGCCAAC
    ATCCCTATTA TAACAGAGCT AGTAAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT
    GATGATGATC CAGATACAGA ACTGTACCTC ACGCAGCCCT TTGCATGTGG AACCGCATTT
    GCTGTCAGCG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA
    CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCCGAG
    GAAAATGCTT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT
    CGAGTTGCTC AGTTGGCTTT GTATGATGGG CCATTTGCAG ACCTAGGGGA TGGAGGCTGC
    TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT
    CGATTAAGGG ACGCTCATCT AAGTACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA
    AACCCACCAT ATGAGTTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTC
    GACCACAATG CTGGCCAGTC CCGGGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT
    TCCAGCAAGA AGAGCTCATC AGTTCACTCC ATCCCATCAA CAGCAAACCG ACCGAACCGC
    ACCAAGACCA GGGATTCCCG GGAAAAACAG AAAAAAGAAA TGGTTTACAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37439
    Clone ID Related Accession (Same CDS sequence) XM_015288166.2 , XM_015288166.1
    Accession Version XM_015288166.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3654bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCCC
    AAGATGTCCA TCTACAAGAG AATGAAACTG GCATGTTGTT TTGATTGCGG ACGCTCTGAG
    CGTGACTGCT CTTGCATGTC AGGCAGTGTA CATAGTAACA TGGACACCCT TGAGAGAGCC
    TTCCCACTTT CTTCTGTCTC TGTTAATGAT TGCTCCACCA GTTTACGTGC CTTTGAAGAT
    GAGCAGCCGT CAACACTATC ACCCAAAAAA AAGCAACGAA ATGGAGGCAT GCGAAATTCA
    CCCAACTCCT CACCCAAACT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAATGAACAG
    ATTGACAATA TGGATGCCAA TGTGAAAAAA TATGACTCTA CGGGAATGTT TCATTGGTGT
    CCAGCCAAGG ATATTGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA
    AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA
    AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG
    TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CCCTGCATAA CTTCCCCAAG
    GTTTCAATCT TGCCTGGTAC ACCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC
    CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG
    CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC
    ATTGGGGTCT TGCAGGCTAA TTCCCAAGGC TTTACACCCC CTGGTATGGA TAGGTCATCT
    CCAGACAACA GTCCTGTCCA CGGCCTGTTA CGTCAACCCT CCATTACAAC AGGAGCCAAC
    ATCCCTATTA TAACAGAGCT AGTAAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT
    GATGATGATC CAGATACAGA ACTGTACCTC ACGCAGCCCT TTGCATGTGG AACCGCATTT
    GCTGTCAGCG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA
    CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCCGAG
    GAAAATGCTT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT
    CGAGTTGCTC AGTTGGCTTT GTATGATGGG CCATTTGCAG ACCTAGGGGA TGGAGGCTGC
    TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT
    CGATTAAGGG ACGCTCATCT AAGTACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA
    AACCCACCAT ATGAGTTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTC
    GACCACAATG CTGGCCAGTC CCGGGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT
    TCCAGCAAGA AGAGCTCATC AGTTCACTCC ATCCCATCAA CAGCAAACCG ACCGAACCGC
    ACCAAGACCA GGGATTCCCG GGAAAAACAG AAAAAAGAAA TGGTTTACAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143652.1
    CDS373..4026
    Translation

    Target ORF information:

    RefSeq Version XM_015288166.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288166.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCCC
    AAGATGTCCA TCTACAAGAG AATGAAACTG GCATGTTGTT TTGATTGCGG ACGCTCTGAG
    CGTGACTGCT CTTGCATGTC AGGCAGTGTA CATAGTAACA TGGACACCCT TGAGAGAGCC
    TTCCCACTTT CTTCTGTCTC TGTTAATGAT TGCTCCACCA GTTTACGTGC CTTTGAAGAT
    GAGCAGCCGT CAACACTATC ACCCAAAAAA AAGCAACGAA ATGGAGGCAT GCGAAATTCA
    CCCAACTCCT CACCCAAACT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAATGAACAG
    ATTGACAATA TGGATGCCAA TGTGAAAAAA TATGACTCTA CGGGAATGTT TCATTGGTGT
    CCAGCCAAGG ATATTGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA
    AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA
    AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG
    TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CCCTGCATAA CTTCCCCAAG
    GTTTCAATCT TGCCTGGTAC ACCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC
    CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG
    CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC
    ATTGGGGTCT TGCAGGCTAA TTCCCAAGGC TTTACACCCC CTGGTATGGA TAGGTCATCT
    CCAGACAACA GTCCTGTCCA CGGCCTGTTA CGTCAACCCT CCATTACAAC AGGAGCCAAC
    ATCCCTATTA TAACAGAGCT AGTAAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT
    GATGATGATC CAGATACAGA ACTGTACCTC ACGCAGCCCT TTGCATGTGG AACCGCATTT
    GCTGTCAGCG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA
    CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCCGAG
    GAAAATGCTT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT
    CGAGTTGCTC AGTTGGCTTT GTATGATGGG CCATTTGCAG ACCTAGGGGA TGGAGGCTGC
    TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT
    CGATTAAGGG ACGCTCATCT AAGTACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA
    AACCCACCAT ATGAGTTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTC
    GACCACAATG CTGGCCAGTC CCGGGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT
    TCCAGCAAGA AGAGCTCATC AGTTCACTCC ATCCCATCAA CAGCAAACCG ACCGAACCGC
    ACCAAGACCA GGGATTCCCG GGAAAAACAG AAAAAAGAAA TGGTTTACAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37440
    Clone ID Related Accession (Same CDS sequence) XM_015288167.1
    Accession Version XM_015288167.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTTTG AAGATGAGCA GCCGTCAACA CTATCACCCA AAAAAAAGCA ACGAAATGGA
    GGCATGCGAA ATTCACCCAA CTCCTCACCC AAACTGATGA GGCATGACCC CTTGTTAATT
    CCTGGCAATG AACAGATTGA CAATATGGAT GCCAATGTGA AAAAATATGA CTCTACGGGA
    ATGTTTCATT GGTGTCCAGC CAAGGATATT GAAAAGGTCA TTTTGACTCG AAGTGAAGCA
    GCGATGACAG TTTTAAGTGG GCATGTAGTC GTTTGCATAT TTGGGGATGT TAAATCTGCT
    TTGATTGGAT TAAGAAATTT AGTGATGCCA TTACGAGCCA GCAACTTTCA CTACCATGAA
    CTCAAGCACA TTGTGTTTGT GGGTTCACTT GAATACCTGA GAAGGGAATG GGAGACCCTG
    CATAACTTCC CCAAGGTTTC AATCTTGCCT GGTACACCAT TAAGTCGGGC TGATTTAAGG
    GCTGTCAACA TCAACCTCTG CGACATGTGC GTTATCCTGT CAGCCAATCA GAATAATATT
    GATGATGCTT CGCTGCAGGA CAAGGAATGC ATCTTGGCGT CACTCAACAT CAAATCTATG
    CAGTTTGATG ACAGCATTGG GGTCTTGCAG GCTAATTCCC AAGGCTTTAC ACCCCCTGGT
    ATGGATAGGT CATCTCCAGA CAACAGTCCT GTCCACGGCC TGTTACGTCA ACCCTCCATT
    ACAACAGGAG CCAACATCCC TATTATAACA GAGCTAGTAA ATGATTCAAA TGTTCAGTTC
    TTGGACCAAG ATGATGATGA TGATCCAGAT ACAGAACTGT ACCTCACGCA GCCCTTTGCA
    TGTGGAACCG CATTTGCTGT CAGCGTGCTG GACTCTCTCA TGAGCGCAAC ATACTTCAAT
    GACAATATCC TCACACTGAT ACGGACATTG GTAACAGGAG GAGCCACACC AGAGCTGGAA
    GCACTGATTG CCGAGGAAAA TGCTTTGAGA GGAGGTTACA GCACGCCCCA GACACTTGCA
    AACAGGGACA GGTGTCGAGT TGCTCAGTTG GCTTTGTATG ATGGGCCATT TGCAGACCTA
    GGGGATGGAG GCTGCTATGG AGATCTATTT TGTAAAGCAC TGAAAACATA CAATATGCTT
    TGCTTTGGAA TTTATCGATT AAGGGACGCT CATCTAAGTA CTCCCAGCCA ATGCACTAAA
    CGTTATGTTA TCACAAACCC ACCATATGAG TTTGAGCTTG TGCCGACAGA CTTGATCTTC
    TGTTTGATGC AATTCGACCA CAATGCTGGC CAGTCCCGGG CCAGTCTTTC TCATTCCTCC
    CATTCCTCCT ATTCTTCCAG CAAGAAGAGC TCATCAGTTC ACTCCATCCC ATCAACAGCA
    AACCGACCGA ACCGCACCAA GACCAGGGAT TCCCGGGAAA AACAGAATGC AACAAGGATG
    AATAGAATGG GCCAAGCAGA AAAGAAATGG TTTACAGATG AACCGGATAA TGCCTATCCC
    AGAAACATTC AAATCAAGCC CATGAGCACC CACATGGCCA ATCAGATCAA TCAATACAAA
    TCGACAAGCA GCTTGATACC ACCAATCAGA GAAGTTGAAG ATGAATGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143653.1
    CDS116..3766
    Translation

    Target ORF information:

    RefSeq Version XM_015288167.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288167.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTTTG AAGATGAGCA GCCGTCAACA CTATCACCCA AAAAAAAGCA ACGAAATGGA
    GGCATGCGAA ATTCACCCAA CTCCTCACCC AAACTGATGA GGCATGACCC CTTGTTAATT
    CCTGGCAATG AACAGATTGA CAATATGGAT GCCAATGTGA AAAAATATGA CTCTACGGGA
    ATGTTTCATT GGTGTCCAGC CAAGGATATT GAAAAGGTCA TTTTGACTCG AAGTGAAGCA
    GCGATGACAG TTTTAAGTGG GCATGTAGTC GTTTGCATAT TTGGGGATGT TAAATCTGCT
    TTGATTGGAT TAAGAAATTT AGTGATGCCA TTACGAGCCA GCAACTTTCA CTACCATGAA
    CTCAAGCACA TTGTGTTTGT GGGTTCACTT GAATACCTGA GAAGGGAATG GGAGACCCTG
    CATAACTTCC CCAAGGTTTC AATCTTGCCT GGTACACCAT TAAGTCGGGC TGATTTAAGG
    GCTGTCAACA TCAACCTCTG CGACATGTGC GTTATCCTGT CAGCCAATCA GAATAATATT
    GATGATGCTT CGCTGCAGGA CAAGGAATGC ATCTTGGCGT CACTCAACAT CAAATCTATG
    CAGTTTGATG ACAGCATTGG GGTCTTGCAG GCTAATTCCC AAGGCTTTAC ACCCCCTGGT
    ATGGATAGGT CATCTCCAGA CAACAGTCCT GTCCACGGCC TGTTACGTCA ACCCTCCATT
    ACAACAGGAG CCAACATCCC TATTATAACA GAGCTAGTAA ATGATTCAAA TGTTCAGTTC
    TTGGACCAAG ATGATGATGA TGATCCAGAT ACAGAACTGT ACCTCACGCA GCCCTTTGCA
    TGTGGAACCG CATTTGCTGT CAGCGTGCTG GACTCTCTCA TGAGCGCAAC ATACTTCAAT
    GACAATATCC TCACACTGAT ACGGACATTG GTAACAGGAG GAGCCACACC AGAGCTGGAA
    GCACTGATTG CCGAGGAAAA TGCTTTGAGA GGAGGTTACA GCACGCCCCA GACACTTGCA
    AACAGGGACA GGTGTCGAGT TGCTCAGTTG GCTTTGTATG ATGGGCCATT TGCAGACCTA
    GGGGATGGAG GCTGCTATGG AGATCTATTT TGTAAAGCAC TGAAAACATA CAATATGCTT
    TGCTTTGGAA TTTATCGATT AAGGGACGCT CATCTAAGTA CTCCCAGCCA ATGCACTAAA
    CGTTATGTTA TCACAAACCC ACCATATGAG TTTGAGCTTG TGCCGACAGA CTTGATCTTC
    TGTTTGATGC AATTCGACCA CAATGCTGGC CAGTCCCGGG CCAGTCTTTC TCATTCCTCC
    CATTCCTCCT ATTCTTCCAG CAAGAAGAGC TCATCAGTTC ACTCCATCCC ATCAACAGCA
    AACCGACCGA ACCGCACCAA GACCAGGGAT TCCCGGGAAA AACAGAATGC AACAAGGATG
    AATAGAATGG GCCAAGCAGA AAAGAAATGG TTTACAGATG AACCGGATAA TGCCTATCCC
    AGAAACATTC AAATCAAGCC CATGAGCACC CACATGGCCA ATCAGATCAA TCAATACAAA
    TCGACAAGCA GCTTGATACC ACCAATCAGA GAAGTTGAAG ATGAATGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37441
    Clone ID Related Accession (Same CDS sequence) XM_015288168.1 , XM_015288168.2
    Accession Version XM_015288168.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3648bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTTTG AAGATGAGCA GCCGTCAACA CTATCACCCA AAAAAAAGCA ACGAAATGGA
    GGCATGCGAA ATTCACCCAA CTCCTCACCC AAACTGATGA GGCATGACCC CTTGTTAATT
    CCTGGCAATG AACAGATTGA CAATATGGAT GCCAATGTGA AAAAATATGA CTCTACGGGA
    ATGTTTCATT GGTGTCCAGC CAAGGATATT GAAAAGGTCA TTTTGACTCG AAGTGAAGCA
    GCGATGACAG TTTTAAGTGG GCATGTAGTC GTTTGCATAT TTGGGGATGT TAAATCTGCT
    TTGATTGGAT TAAGAAATTT AGTGATGCCA TTACGAGCCA GCAACTTTCA CTACCATGAA
    CTCAAGCACA TTGTGTTTGT GGGTTCACTT GAATACCTGA GAAGGGAATG GGAGACCCTG
    CATAACTTCC CCAAGGTTTC AATCTTGCCT GGTACACCAT TAAGTCGGGC TGATTTAAGG
    GCTGTCAACA TCAACCTCTG CGACATGTGC GTTATCCTGT CAGCCAATCA GAATAATATT
    GATGATGCTT CGCTGCAGGA CAAGGAATGC ATCTTGGCGT CACTCAACAT CAAATCTATG
    CAGTTTGATG ACAGCATTGG GGTCTTGCAG GCTAATTCCC AAGGCTTTAC ACCCCCTGGT
    ATGGATAGGT CATCTCCAGA CAACAGTCCT GTCCACGGCC TGTTACGTCA ACCCTCCATT
    ACAACAGGAG CCAACATCCC TATTATAACA GAGCTAGTAA ATGATTCAAA TGTTCAGTTC
    TTGGACCAAG ATGATGATGA TGATCCAGAT ACAGAACTGT ACCTCACGCA GCCCTTTGCA
    TGTGGAACCG CATTTGCTGT CAGCGTGCTG GACTCTCTCA TGAGCGCAAC ATACTTCAAT
    GACAATATCC TCACACTGAT ACGGACATTG GTAACAGGAG GAGCCACACC AGAGCTGGAA
    GCACTGATTG CCGAGGAAAA TGCTTTGAGA GGAGGTTACA GCACGCCCCA GACACTTGCA
    AACAGGGACA GGTGTCGAGT TGCTCAGTTG GCTTTGTATG ATGGGCCATT TGCAGACCTA
    GGGGATGGAG GCTGCTATGG AGATCTATTT TGTAAAGCAC TGAAAACATA CAATATGCTT
    TGCTTTGGAA TTTATCGATT AAGGGACGCT CATCTAAGTA CTCCCAGCCA ATGCACTAAA
    CGTTATGTTA TCACAAACCC ACCATATGAG TTTGAGCTTG TGCCGACAGA CTTGATCTTC
    TGTTTGATGC AATTCGACCA CAATGCTGGC CAGTCCCGGG CCAGTCTTTC TCATTCCTCC
    CATTCCTCCT ATTCTTCCAG CAAGAAGAGC TCATCAGTTC ACTCCATCCC ATCAACAGCA
    AACCGACCGA ACCGCACCAA GACCAGGGAT TCCCGGGAAA AACAGAATGC AACAAGGATG
    AATAGAATGG GCCAAGAAAA GAAATGGTTT ACAGATGAAC CGGATAATGC CTATCCCAGA
    AACATTCAAA TCAAGCCCAT GAGCACCCAC ATGGCCAATC AGATCAATCA ATACAAATCG
    ACAAGCAGCT TGATACCACC AATCAGAGAA GTTGAAGATG AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143654.1
    CDS373..4020
    Translation

    Target ORF information:

    RefSeq Version XM_015288168.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288168.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTTTG AAGATGAGCA GCCGTCAACA CTATCACCCA AAAAAAAGCA ACGAAATGGA
    GGCATGCGAA ATTCACCCAA CTCCTCACCC AAACTGATGA GGCATGACCC CTTGTTAATT
    CCTGGCAATG AACAGATTGA CAATATGGAT GCCAATGTGA AAAAATATGA CTCTACGGGA
    ATGTTTCATT GGTGTCCAGC CAAGGATATT GAAAAGGTCA TTTTGACTCG AAGTGAAGCA
    GCGATGACAG TTTTAAGTGG GCATGTAGTC GTTTGCATAT TTGGGGATGT TAAATCTGCT
    TTGATTGGAT TAAGAAATTT AGTGATGCCA TTACGAGCCA GCAACTTTCA CTACCATGAA
    CTCAAGCACA TTGTGTTTGT GGGTTCACTT GAATACCTGA GAAGGGAATG GGAGACCCTG
    CATAACTTCC CCAAGGTTTC AATCTTGCCT GGTACACCAT TAAGTCGGGC TGATTTAAGG
    GCTGTCAACA TCAACCTCTG CGACATGTGC GTTATCCTGT CAGCCAATCA GAATAATATT
    GATGATGCTT CGCTGCAGGA CAAGGAATGC ATCTTGGCGT CACTCAACAT CAAATCTATG
    CAGTTTGATG ACAGCATTGG GGTCTTGCAG GCTAATTCCC AAGGCTTTAC ACCCCCTGGT
    ATGGATAGGT CATCTCCAGA CAACAGTCCT GTCCACGGCC TGTTACGTCA ACCCTCCATT
    ACAACAGGAG CCAACATCCC TATTATAACA GAGCTAGTAA ATGATTCAAA TGTTCAGTTC
    TTGGACCAAG ATGATGATGA TGATCCAGAT ACAGAACTGT ACCTCACGCA GCCCTTTGCA
    TGTGGAACCG CATTTGCTGT CAGCGTGCTG GACTCTCTCA TGAGCGCAAC ATACTTCAAT
    GACAATATCC TCACACTGAT ACGGACATTG GTAACAGGAG GAGCCACACC AGAGCTGGAA
    GCACTGATTG CCGAGGAAAA TGCTTTGAGA GGAGGTTACA GCACGCCCCA GACACTTGCA
    AACAGGGACA GGTGTCGAGT TGCTCAGTTG GCTTTGTATG ATGGGCCATT TGCAGACCTA
    GGGGATGGAG GCTGCTATGG AGATCTATTT TGTAAAGCAC TGAAAACATA CAATATGCTT
    TGCTTTGGAA TTTATCGATT AAGGGACGCT CATCTAAGTA CTCCCAGCCA ATGCACTAAA
    CGTTATGTTA TCACAAACCC ACCATATGAG TTTGAGCTTG TGCCGACAGA CTTGATCTTC
    TGTTTGATGC AATTCGACCA CAATGCTGGC CAGTCCCGGG CCAGTCTTTC TCATTCCTCC
    CATTCCTCCT ATTCTTCCAG CAAGAAGAGC TCATCAGTTC ACTCCATCCC ATCAACAGCA
    AACCGACCGA ACCGCACCAA GACCAGGGAT TCCCGGGAAA AACAGAATGC AACAAGGATG
    AATAGAATGG GCCAAGAAAA GAAATGGTTT ACAGATGAAC CGGATAATGC CTATCCCAGA
    AACATTCAAA TCAAGCCCAT GAGCACCCAC ATGGCCAATC AGATCAATCA ATACAAATCG
    ACAAGCAGCT TGATACCACC AATCAGAGAA GTTGAAGATG AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37441
    Clone ID Related Accession (Same CDS sequence) XM_015288168.1 , XM_015288168.2
    Accession Version XM_015288168.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3648bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288168.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTTTG AAGATGAGCA GCCGTCAACA CTATCACCCA AAAAAAAGCA ACGAAATGGA
    GGCATGCGAA ATTCACCCAA CTCCTCACCC AAACTGATGA GGCATGACCC CTTGTTAATT
    CCTGGCAATG AACAGATTGA CAATATGGAT GCCAATGTGA AAAAATATGA CTCTACGGGA
    ATGTTTCATT GGTGTCCAGC CAAGGATATT GAAAAGGTCA TTTTGACTCG AAGTGAAGCA
    GCGATGACAG TTTTAAGTGG GCATGTAGTC GTTTGCATAT TTGGGGATGT TAAATCTGCT
    TTGATTGGAT TAAGAAATTT AGTGATGCCA TTACGAGCCA GCAACTTTCA CTACCATGAA
    CTCAAGCACA TTGTGTTTGT GGGTTCACTT GAATACCTGA GAAGGGAATG GGAGACCCTG
    CATAACTTCC CCAAGGTTTC AATCTTGCCT GGTACACCAT TAAGTCGGGC TGATTTAAGG
    GCTGTCAACA TCAACCTCTG CGACATGTGC GTTATCCTGT CAGCCAATCA GAATAATATT
    GATGATGCTT CGCTGCAGGA CAAGGAATGC ATCTTGGCGT CACTCAACAT CAAATCTATG
    CAGTTTGATG ACAGCATTGG GGTCTTGCAG GCTAATTCCC AAGGCTTTAC ACCCCCTGGT
    ATGGATAGGT CATCTCCAGA CAACAGTCCT GTCCACGGCC TGTTACGTCA ACCCTCCATT
    ACAACAGGAG CCAACATCCC TATTATAACA GAGCTAGTAA ATGATTCAAA TGTTCAGTTC
    TTGGACCAAG ATGATGATGA TGATCCAGAT ACAGAACTGT ACCTCACGCA GCCCTTTGCA
    TGTGGAACCG CATTTGCTGT CAGCGTGCTG GACTCTCTCA TGAGCGCAAC ATACTTCAAT
    GACAATATCC TCACACTGAT ACGGACATTG GTAACAGGAG GAGCCACACC AGAGCTGGAA
    GCACTGATTG CCGAGGAAAA TGCTTTGAGA GGAGGTTACA GCACGCCCCA GACACTTGCA
    AACAGGGACA GGTGTCGAGT TGCTCAGTTG GCTTTGTATG ATGGGCCATT TGCAGACCTA
    GGGGATGGAG GCTGCTATGG AGATCTATTT TGTAAAGCAC TGAAAACATA CAATATGCTT
    TGCTTTGGAA TTTATCGATT AAGGGACGCT CATCTAAGTA CTCCCAGCCA ATGCACTAAA
    CGTTATGTTA TCACAAACCC ACCATATGAG TTTGAGCTTG TGCCGACAGA CTTGATCTTC
    TGTTTGATGC AATTCGACCA CAATGCTGGC CAGTCCCGGG CCAGTCTTTC TCATTCCTCC
    CATTCCTCCT ATTCTTCCAG CAAGAAGAGC TCATCAGTTC ACTCCATCCC ATCAACAGCA
    AACCGACCGA ACCGCACCAA GACCAGGGAT TCCCGGGAAA AACAGAATGC AACAAGGATG
    AATAGAATGG GCCAAGAAAA GAAATGGTTT ACAGATGAAC CGGATAATGC CTATCCCAGA
    AACATTCAAA TCAAGCCCAT GAGCACCCAC ATGGCCAATC AGATCAATCA ATACAAATCG
    ACAAGCAGCT TGATACCACC AATCAGAGAA GTTGAAGATG AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143654.1
    CDS368..4015
    Translation

    Target ORF information:

    RefSeq Version XM_015288168.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288168.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTTTG AAGATGAGCA GCCGTCAACA CTATCACCCA AAAAAAAGCA ACGAAATGGA
    GGCATGCGAA ATTCACCCAA CTCCTCACCC AAACTGATGA GGCATGACCC CTTGTTAATT
    CCTGGCAATG AACAGATTGA CAATATGGAT GCCAATGTGA AAAAATATGA CTCTACGGGA
    ATGTTTCATT GGTGTCCAGC CAAGGATATT GAAAAGGTCA TTTTGACTCG AAGTGAAGCA
    GCGATGACAG TTTTAAGTGG GCATGTAGTC GTTTGCATAT TTGGGGATGT TAAATCTGCT
    TTGATTGGAT TAAGAAATTT AGTGATGCCA TTACGAGCCA GCAACTTTCA CTACCATGAA
    CTCAAGCACA TTGTGTTTGT GGGTTCACTT GAATACCTGA GAAGGGAATG GGAGACCCTG
    CATAACTTCC CCAAGGTTTC AATCTTGCCT GGTACACCAT TAAGTCGGGC TGATTTAAGG
    GCTGTCAACA TCAACCTCTG CGACATGTGC GTTATCCTGT CAGCCAATCA GAATAATATT
    GATGATGCTT CGCTGCAGGA CAAGGAATGC ATCTTGGCGT CACTCAACAT CAAATCTATG
    CAGTTTGATG ACAGCATTGG GGTCTTGCAG GCTAATTCCC AAGGCTTTAC ACCCCCTGGT
    ATGGATAGGT CATCTCCAGA CAACAGTCCT GTCCACGGCC TGTTACGTCA ACCCTCCATT
    ACAACAGGAG CCAACATCCC TATTATAACA GAGCTAGTAA ATGATTCAAA TGTTCAGTTC
    TTGGACCAAG ATGATGATGA TGATCCAGAT ACAGAACTGT ACCTCACGCA GCCCTTTGCA
    TGTGGAACCG CATTTGCTGT CAGCGTGCTG GACTCTCTCA TGAGCGCAAC ATACTTCAAT
    GACAATATCC TCACACTGAT ACGGACATTG GTAACAGGAG GAGCCACACC AGAGCTGGAA
    GCACTGATTG CCGAGGAAAA TGCTTTGAGA GGAGGTTACA GCACGCCCCA GACACTTGCA
    AACAGGGACA GGTGTCGAGT TGCTCAGTTG GCTTTGTATG ATGGGCCATT TGCAGACCTA
    GGGGATGGAG GCTGCTATGG AGATCTATTT TGTAAAGCAC TGAAAACATA CAATATGCTT
    TGCTTTGGAA TTTATCGATT AAGGGACGCT CATCTAAGTA CTCCCAGCCA ATGCACTAAA
    CGTTATGTTA TCACAAACCC ACCATATGAG TTTGAGCTTG TGCCGACAGA CTTGATCTTC
    TGTTTGATGC AATTCGACCA CAATGCTGGC CAGTCCCGGG CCAGTCTTTC TCATTCCTCC
    CATTCCTCCT ATTCTTCCAG CAAGAAGAGC TCATCAGTTC ACTCCATCCC ATCAACAGCA
    AACCGACCGA ACCGCACCAA GACCAGGGAT TCCCGGGAAA AACAGAATGC AACAAGGATG
    AATAGAATGG GCCAAGAAAA GAAATGGTTT ACAGATGAAC CGGATAATGC CTATCCCAGA
    AACATTCAAA TCAAGCCCAT GAGCACCCAC ATGGCCAATC AGATCAATCA ATACAAATCG
    ACAAGCAGCT TGATACCACC AATCAGAGAA GTTGAAGATG AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37442
    Clone ID Related Accession (Same CDS sequence) XM_015288169.1 , XM_015288169.2
    Accession Version XM_015288169.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3648bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCAAT GTTTGCTCGC
    TACGTGCCAG AAATTGCTGC TCTCATCCTT AATCGGAAGA AATACGGCGG GACTTTTAAC
    TCAACCCGAG GCAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTTTG AAGATGAGCA GCCGTCAACA CTATCACCCA AAAAAAAGCA ACGAAATGGA
    GGCATGCGAA ATTCACCCAA CTCCTCACCC AAACTGATGA GGCATGACCC CTTGTTAATT
    CCTGGCAATG AACAGATTGA CAATATGGAT GCCAATGTGA AAAAATATGA CTCTACGGGA
    ATGTTTCATT GGTGTCCAGC CAAGGATATT GAAAAGGTCA TTTTGACTCG AAGTGAAGCA
    GCGATGACAG TTTTAAGTGG GCATGTAGTC GTTTGCATAT TTGGGGATGT TAAATCTGCT
    TTGATTGGAT TAAGAAATTT AGTGATGCCA TTACGAGCCA GCAACTTTCA CTACCATGAA
    CTCAAGCACA TTGTGTTTGT GGGTTCACTT GAATACCTGA GAAGGGAATG GGAGACCCTG
    CATAACTTCC CCAAGGTTTC AATCTTGCCT GGTACACCAT TAAGTCGGGC TGATTTAAGG
    GCTGTCAACA TCAACCTCTG CGACATGTGC GTTATCCTGT CAGCCAATCA GAATAATATT
    GATGATGCTT CGCTGCAGGA CAAGGAATGC ATCTTGGCGT CACTCAACAT CAAATCTATG
    CAGTTTGATG ACAGCATTGG GGTCTTGCAG GCTAATTCCC AAGGCTTTAC ACCCCCTGGT
    ATGGATAGGT CATCTCCAGA CAACAGTCCT GTCCACGGCC TGTTACGTCA ACCCTCCATT
    ACAACAGGAG CCAACATCCC TATTATAACA GAGCTAGTAA ATGATTCAAA TGTTCAGTTC
    TTGGACCAAG ATGATGATGA TGATCCAGAT ACAGAACTGT ACCTCACGCA GCCCTTTGCA
    TGTGGAACCG CATTTGCTGT CAGCGTGCTG GACTCTCTCA TGAGCGCAAC ATACTTCAAT
    GACAATATCC TCACACTGAT ACGGACATTG GTAACAGGAG GAGCCACACC AGAGCTGGAA
    GCACTGATTG CCGAGGAAAA TGCTTTGAGA GGAGGTTACA GCACGCCCCA GACACTTGCA
    AACAGGGACA GGTGTCGAGT TGCTCAGTTG GCTTTGTATG ATGGGCCATT TGCAGACCTA
    GGGGATGGAG GCTGCTATGG AGATCTATTT TGTAAAGCAC TGAAAACATA CAATATGCTT
    TGCTTTGGAA TTTATCGATT AAGGGACGCT CATCTAAGTA CTCCCAGCCA ATGCACTAAA
    CGTTATGTTA TCACAAACCC ACCATATGAG TTTGAGCTTG TGCCGACAGA CTTGATCTTC
    TGTTTGATGC AATTCGACCA CAATGCTGGC CAGTCCCGGG CCAGTCTTTC TCATTCCTCC
    CATTCCTCCT ATTCTTCCAG CAAGAAGAGC TCATCAGTTC ACTCCATCCC ATCAACAGCA
    AACCGACCGA ACCGCACCAA GACCAGGGAT TCCCGGGAAA AACAGAATGC AACAAGGATG
    AATAGAATGG GCCAAGAAAA GAAATGGTTT ACAGATGAAC CGGATAATGC CTATCCCAGA
    AACATTCAAA TCAAGCCCAT GAGCACCCAC ATGGCCAATC AGATCAATCA ATACAAATCG
    ACAAGCAGCT TGATACCACC AATCAGAGAA GTTGAAGATG AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143655.1
    CDS373..4020
    Translation

    Target ORF information:

    RefSeq Version XM_015288169.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288169.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCAAT GTTTGCTCGC
    TACGTGCCAG AAATTGCTGC TCTCATCCTT AATCGGAAGA AATACGGCGG GACTTTTAAC
    TCAACCCGAG GCAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTTTG AAGATGAGCA GCCGTCAACA CTATCACCCA AAAAAAAGCA ACGAAATGGA
    GGCATGCGAA ATTCACCCAA CTCCTCACCC AAACTGATGA GGCATGACCC CTTGTTAATT
    CCTGGCAATG AACAGATTGA CAATATGGAT GCCAATGTGA AAAAATATGA CTCTACGGGA
    ATGTTTCATT GGTGTCCAGC CAAGGATATT GAAAAGGTCA TTTTGACTCG AAGTGAAGCA
    GCGATGACAG TTTTAAGTGG GCATGTAGTC GTTTGCATAT TTGGGGATGT TAAATCTGCT
    TTGATTGGAT TAAGAAATTT AGTGATGCCA TTACGAGCCA GCAACTTTCA CTACCATGAA
    CTCAAGCACA TTGTGTTTGT GGGTTCACTT GAATACCTGA GAAGGGAATG GGAGACCCTG
    CATAACTTCC CCAAGGTTTC AATCTTGCCT GGTACACCAT TAAGTCGGGC TGATTTAAGG
    GCTGTCAACA TCAACCTCTG CGACATGTGC GTTATCCTGT CAGCCAATCA GAATAATATT
    GATGATGCTT CGCTGCAGGA CAAGGAATGC ATCTTGGCGT CACTCAACAT CAAATCTATG
    CAGTTTGATG ACAGCATTGG GGTCTTGCAG GCTAATTCCC AAGGCTTTAC ACCCCCTGGT
    ATGGATAGGT CATCTCCAGA CAACAGTCCT GTCCACGGCC TGTTACGTCA ACCCTCCATT
    ACAACAGGAG CCAACATCCC TATTATAACA GAGCTAGTAA ATGATTCAAA TGTTCAGTTC
    TTGGACCAAG ATGATGATGA TGATCCAGAT ACAGAACTGT ACCTCACGCA GCCCTTTGCA
    TGTGGAACCG CATTTGCTGT CAGCGTGCTG GACTCTCTCA TGAGCGCAAC ATACTTCAAT
    GACAATATCC TCACACTGAT ACGGACATTG GTAACAGGAG GAGCCACACC AGAGCTGGAA
    GCACTGATTG CCGAGGAAAA TGCTTTGAGA GGAGGTTACA GCACGCCCCA GACACTTGCA
    AACAGGGACA GGTGTCGAGT TGCTCAGTTG GCTTTGTATG ATGGGCCATT TGCAGACCTA
    GGGGATGGAG GCTGCTATGG AGATCTATTT TGTAAAGCAC TGAAAACATA CAATATGCTT
    TGCTTTGGAA TTTATCGATT AAGGGACGCT CATCTAAGTA CTCCCAGCCA ATGCACTAAA
    CGTTATGTTA TCACAAACCC ACCATATGAG TTTGAGCTTG TGCCGACAGA CTTGATCTTC
    TGTTTGATGC AATTCGACCA CAATGCTGGC CAGTCCCGGG CCAGTCTTTC TCATTCCTCC
    CATTCCTCCT ATTCTTCCAG CAAGAAGAGC TCATCAGTTC ACTCCATCCC ATCAACAGCA
    AACCGACCGA ACCGCACCAA GACCAGGGAT TCCCGGGAAA AACAGAATGC AACAAGGATG
    AATAGAATGG GCCAAGAAAA GAAATGGTTT ACAGATGAAC CGGATAATGC CTATCCCAGA
    AACATTCAAA TCAAGCCCAT GAGCACCCAC ATGGCCAATC AGATCAATCA ATACAAATCG
    ACAAGCAGCT TGATACCACC AATCAGAGAA GTTGAAGATG AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37442
    Clone ID Related Accession (Same CDS sequence) XM_015288169.1 , XM_015288169.2
    Accession Version XM_015288169.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3648bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288169.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCAAT GTTTGCTCGC
    TACGTGCCAG AAATTGCTGC TCTCATCCTT AATCGGAAGA AATACGGCGG GACTTTTAAC
    TCAACCCGAG GCAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTTTG AAGATGAGCA GCCGTCAACA CTATCACCCA AAAAAAAGCA ACGAAATGGA
    GGCATGCGAA ATTCACCCAA CTCCTCACCC AAACTGATGA GGCATGACCC CTTGTTAATT
    CCTGGCAATG AACAGATTGA CAATATGGAT GCCAATGTGA AAAAATATGA CTCTACGGGA
    ATGTTTCATT GGTGTCCAGC CAAGGATATT GAAAAGGTCA TTTTGACTCG AAGTGAAGCA
    GCGATGACAG TTTTAAGTGG GCATGTAGTC GTTTGCATAT TTGGGGATGT TAAATCTGCT
    TTGATTGGAT TAAGAAATTT AGTGATGCCA TTACGAGCCA GCAACTTTCA CTACCATGAA
    CTCAAGCACA TTGTGTTTGT GGGTTCACTT GAATACCTGA GAAGGGAATG GGAGACCCTG
    CATAACTTCC CCAAGGTTTC AATCTTGCCT GGTACACCAT TAAGTCGGGC TGATTTAAGG
    GCTGTCAACA TCAACCTCTG CGACATGTGC GTTATCCTGT CAGCCAATCA GAATAATATT
    GATGATGCTT CGCTGCAGGA CAAGGAATGC ATCTTGGCGT CACTCAACAT CAAATCTATG
    CAGTTTGATG ACAGCATTGG GGTCTTGCAG GCTAATTCCC AAGGCTTTAC ACCCCCTGGT
    ATGGATAGGT CATCTCCAGA CAACAGTCCT GTCCACGGCC TGTTACGTCA ACCCTCCATT
    ACAACAGGAG CCAACATCCC TATTATAACA GAGCTAGTAA ATGATTCAAA TGTTCAGTTC
    TTGGACCAAG ATGATGATGA TGATCCAGAT ACAGAACTGT ACCTCACGCA GCCCTTTGCA
    TGTGGAACCG CATTTGCTGT CAGCGTGCTG GACTCTCTCA TGAGCGCAAC ATACTTCAAT
    GACAATATCC TCACACTGAT ACGGACATTG GTAACAGGAG GAGCCACACC AGAGCTGGAA
    GCACTGATTG CCGAGGAAAA TGCTTTGAGA GGAGGTTACA GCACGCCCCA GACACTTGCA
    AACAGGGACA GGTGTCGAGT TGCTCAGTTG GCTTTGTATG ATGGGCCATT TGCAGACCTA
    GGGGATGGAG GCTGCTATGG AGATCTATTT TGTAAAGCAC TGAAAACATA CAATATGCTT
    TGCTTTGGAA TTTATCGATT AAGGGACGCT CATCTAAGTA CTCCCAGCCA ATGCACTAAA
    CGTTATGTTA TCACAAACCC ACCATATGAG TTTGAGCTTG TGCCGACAGA CTTGATCTTC
    TGTTTGATGC AATTCGACCA CAATGCTGGC CAGTCCCGGG CCAGTCTTTC TCATTCCTCC
    CATTCCTCCT ATTCTTCCAG CAAGAAGAGC TCATCAGTTC ACTCCATCCC ATCAACAGCA
    AACCGACCGA ACCGCACCAA GACCAGGGAT TCCCGGGAAA AACAGAATGC AACAAGGATG
    AATAGAATGG GCCAAGAAAA GAAATGGTTT ACAGATGAAC CGGATAATGC CTATCCCAGA
    AACATTCAAA TCAAGCCCAT GAGCACCCAC ATGGCCAATC AGATCAATCA ATACAAATCG
    ACAAGCAGCT TGATACCACC AATCAGAGAA GTTGAAGATG AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143655.1
    CDS368..4015
    Translation

    Target ORF information:

    RefSeq Version XM_015288169.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288169.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCAAT GTTTGCTCGC
    TACGTGCCAG AAATTGCTGC TCTCATCCTT AATCGGAAGA AATACGGCGG GACTTTTAAC
    TCAACCCGAG GCAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTG
    ATCTATTTTG AAGATGAGCA GCCGTCAACA CTATCACCCA AAAAAAAGCA ACGAAATGGA
    GGCATGCGAA ATTCACCCAA CTCCTCACCC AAACTGATGA GGCATGACCC CTTGTTAATT
    CCTGGCAATG AACAGATTGA CAATATGGAT GCCAATGTGA AAAAATATGA CTCTACGGGA
    ATGTTTCATT GGTGTCCAGC CAAGGATATT GAAAAGGTCA TTTTGACTCG AAGTGAAGCA
    GCGATGACAG TTTTAAGTGG GCATGTAGTC GTTTGCATAT TTGGGGATGT TAAATCTGCT
    TTGATTGGAT TAAGAAATTT AGTGATGCCA TTACGAGCCA GCAACTTTCA CTACCATGAA
    CTCAAGCACA TTGTGTTTGT GGGTTCACTT GAATACCTGA GAAGGGAATG GGAGACCCTG
    CATAACTTCC CCAAGGTTTC AATCTTGCCT GGTACACCAT TAAGTCGGGC TGATTTAAGG
    GCTGTCAACA TCAACCTCTG CGACATGTGC GTTATCCTGT CAGCCAATCA GAATAATATT
    GATGATGCTT CGCTGCAGGA CAAGGAATGC ATCTTGGCGT CACTCAACAT CAAATCTATG
    CAGTTTGATG ACAGCATTGG GGTCTTGCAG GCTAATTCCC AAGGCTTTAC ACCCCCTGGT
    ATGGATAGGT CATCTCCAGA CAACAGTCCT GTCCACGGCC TGTTACGTCA ACCCTCCATT
    ACAACAGGAG CCAACATCCC TATTATAACA GAGCTAGTAA ATGATTCAAA TGTTCAGTTC
    TTGGACCAAG ATGATGATGA TGATCCAGAT ACAGAACTGT ACCTCACGCA GCCCTTTGCA
    TGTGGAACCG CATTTGCTGT CAGCGTGCTG GACTCTCTCA TGAGCGCAAC ATACTTCAAT
    GACAATATCC TCACACTGAT ACGGACATTG GTAACAGGAG GAGCCACACC AGAGCTGGAA
    GCACTGATTG CCGAGGAAAA TGCTTTGAGA GGAGGTTACA GCACGCCCCA GACACTTGCA
    AACAGGGACA GGTGTCGAGT TGCTCAGTTG GCTTTGTATG ATGGGCCATT TGCAGACCTA
    GGGGATGGAG GCTGCTATGG AGATCTATTT TGTAAAGCAC TGAAAACATA CAATATGCTT
    TGCTTTGGAA TTTATCGATT AAGGGACGCT CATCTAAGTA CTCCCAGCCA ATGCACTAAA
    CGTTATGTTA TCACAAACCC ACCATATGAG TTTGAGCTTG TGCCGACAGA CTTGATCTTC
    TGTTTGATGC AATTCGACCA CAATGCTGGC CAGTCCCGGG CCAGTCTTTC TCATTCCTCC
    CATTCCTCCT ATTCTTCCAG CAAGAAGAGC TCATCAGTTC ACTCCATCCC ATCAACAGCA
    AACCGACCGA ACCGCACCAA GACCAGGGAT TCCCGGGAAA AACAGAATGC AACAAGGATG
    AATAGAATGG GCCAAGAAAA GAAATGGTTT ACAGATGAAC CGGATAATGC CTATCCCAGA
    AACATTCAAA TCAAGCCCAT GAGCACCCAC ATGGCCAATC AGATCAATCA ATACAAATCG
    ACAAGCAGCT TGATACCACC AATCAGAGAA GTTGAAGATG AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37443
    Clone ID Related Accession (Same CDS sequence) XM_015288170.1 , XM_015288170.2
    Accession Version XM_015288170.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3645bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCCCAA GATGTCCATC
    TACAAGAGAA TGAAACTGGC ATGTTGTTTT GATTGCGGAC GCTCTGAGCG TGACTGCTCT
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACACCCTTG AGAGAGCCTT CCCACTTTCT
    TCTGTCTCTG TTAATGATTG CTCCACCAGT TTACGTGCCT TTGAAGATGA GCAGCCGTCA
    ACACTATCAC CCAAAAAAAA GCAACGAAAT GGAGGCATGC GAAATTCACC CAACTCCTCA
    CCCAAACTGA TGAGGCATGA CCCCTTGTTA ATTCCTGGCA ATGAACAGAT TGACAATATG
    GATGCCAATG TGAAAAAATA TGACTCTACG GGAATGTTTC ATTGGTGTCC AGCCAAGGAT
    ATTGAAAAGG TCATTTTGAC TCGAAGTGAA GCAGCGATGA CAGTTTTAAG TGGGCATGTA
    GTCGTTTGCA TATTTGGGGA TGTTAAATCT GCTTTGATTG GATTAAGAAA TTTAGTGATG
    CCATTACGAG CCAGCAACTT TCACTACCAT GAACTCAAGC ACATTGTGTT TGTGGGTTCA
    CTTGAATACC TGAGAAGGGA ATGGGAGACC CTGCATAACT TCCCCAAGGT TTCAATCTTG
    CCTGGTACAC CATTAAGTCG GGCTGATTTA AGGGCTGTCA ACATCAACCT CTGCGACATG
    TGCGTTATCC TGTCAGCCAA TCAGAATAAT ATTGATGATG CTTCGCTGCA GGACAAGGAA
    TGCATCTTGG CGTCACTCAA CATCAAATCT ATGCAGTTTG ATGACAGCAT TGGGGTCTTG
    CAGGCTAATT CCCAAGGCTT TACACCCCCT GGTATGGATA GGTCATCTCC AGACAACAGT
    CCTGTCCACG GCCTGTTACG TCAACCCTCC ATTACAACAG GAGCCAACAT CCCTATTATA
    ACAGAGCTAG TAAATGATTC AAATGTTCAG TTCTTGGACC AAGATGATGA TGATGATCCA
    GATACAGAAC TGTACCTCAC GCAGCCCTTT GCATGTGGAA CCGCATTTGC TGTCAGCGTG
    CTGGACTCTC TCATGAGCGC AACATACTTC AATGACAATA TCCTCACACT GATACGGACA
    TTGGTAACAG GAGGAGCCAC ACCAGAGCTG GAAGCACTGA TTGCCGAGGA AAATGCTTTG
    AGAGGAGGTT ACAGCACGCC CCAGACACTT GCAAACAGGG ACAGGTGTCG AGTTGCTCAG
    TTGGCTTTGT ATGATGGGCC ATTTGCAGAC CTAGGGGATG GAGGCTGCTA TGGAGATCTA
    TTTTGTAAAG CACTGAAAAC ATACAATATG CTTTGCTTTG GAATTTATCG ATTAAGGGAC
    GCTCATCTAA GTACTCCCAG CCAATGCACT AAACGTTATG TTATCACAAA CCCACCATAT
    GAGTTTGAGC TTGTGCCGAC AGACTTGATC TTCTGTTTGA TGCAATTCGA CCACAATGCT
    GGCCAGTCCC GGGCCAGTCT TTCTCATTCC TCCCATTCCT CCTATTCTTC CAGCAAGAAG
    AGCTCATCAG TTCACTCCAT CCCATCAACA GCAAACCGAC CGAACCGCAC CAAGACCAGG
    GATTCCCGGG AAAAACAGAA GTATGTGCAG GAAGATAGAC TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143656.1
    CDS116..3760
    Translation

    Target ORF information:

    RefSeq Version XM_015288170.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288170.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCCCAA GATGTCCATC
    TACAAGAGAA TGAAACTGGC ATGTTGTTTT GATTGCGGAC GCTCTGAGCG TGACTGCTCT
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACACCCTTG AGAGAGCCTT CCCACTTTCT
    TCTGTCTCTG TTAATGATTG CTCCACCAGT TTACGTGCCT TTGAAGATGA GCAGCCGTCA
    ACACTATCAC CCAAAAAAAA GCAACGAAAT GGAGGCATGC GAAATTCACC CAACTCCTCA
    CCCAAACTGA TGAGGCATGA CCCCTTGTTA ATTCCTGGCA ATGAACAGAT TGACAATATG
    GATGCCAATG TGAAAAAATA TGACTCTACG GGAATGTTTC ATTGGTGTCC AGCCAAGGAT
    ATTGAAAAGG TCATTTTGAC TCGAAGTGAA GCAGCGATGA CAGTTTTAAG TGGGCATGTA
    GTCGTTTGCA TATTTGGGGA TGTTAAATCT GCTTTGATTG GATTAAGAAA TTTAGTGATG
    CCATTACGAG CCAGCAACTT TCACTACCAT GAACTCAAGC ACATTGTGTT TGTGGGTTCA
    CTTGAATACC TGAGAAGGGA ATGGGAGACC CTGCATAACT TCCCCAAGGT TTCAATCTTG
    CCTGGTACAC CATTAAGTCG GGCTGATTTA AGGGCTGTCA ACATCAACCT CTGCGACATG
    TGCGTTATCC TGTCAGCCAA TCAGAATAAT ATTGATGATG CTTCGCTGCA GGACAAGGAA
    TGCATCTTGG CGTCACTCAA CATCAAATCT ATGCAGTTTG ATGACAGCAT TGGGGTCTTG
    CAGGCTAATT CCCAAGGCTT TACACCCCCT GGTATGGATA GGTCATCTCC AGACAACAGT
    CCTGTCCACG GCCTGTTACG TCAACCCTCC ATTACAACAG GAGCCAACAT CCCTATTATA
    ACAGAGCTAG TAAATGATTC AAATGTTCAG TTCTTGGACC AAGATGATGA TGATGATCCA
    GATACAGAAC TGTACCTCAC GCAGCCCTTT GCATGTGGAA CCGCATTTGC TGTCAGCGTG
    CTGGACTCTC TCATGAGCGC AACATACTTC AATGACAATA TCCTCACACT GATACGGACA
    TTGGTAACAG GAGGAGCCAC ACCAGAGCTG GAAGCACTGA TTGCCGAGGA AAATGCTTTG
    AGAGGAGGTT ACAGCACGCC CCAGACACTT GCAAACAGGG ACAGGTGTCG AGTTGCTCAG
    TTGGCTTTGT ATGATGGGCC ATTTGCAGAC CTAGGGGATG GAGGCTGCTA TGGAGATCTA
    TTTTGTAAAG CACTGAAAAC ATACAATATG CTTTGCTTTG GAATTTATCG ATTAAGGGAC
    GCTCATCTAA GTACTCCCAG CCAATGCACT AAACGTTATG TTATCACAAA CCCACCATAT
    GAGTTTGAGC TTGTGCCGAC AGACTTGATC TTCTGTTTGA TGCAATTCGA CCACAATGCT
    GGCCAGTCCC GGGCCAGTCT TTCTCATTCC TCCCATTCCT CCTATTCTTC CAGCAAGAAG
    AGCTCATCAG TTCACTCCAT CCCATCAACA GCAAACCGAC CGAACCGCAC CAAGACCAGG
    GATTCCCGGG AAAAACAGAA GTATGTGCAG GAAGATAGAC TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37443
    Clone ID Related Accession (Same CDS sequence) XM_015288170.1 , XM_015288170.2
    Accession Version XM_015288170.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3645bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288170.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCCCAA GATGTCCATC
    TACAAGAGAA TGAAACTGGC ATGTTGTTTT GATTGCGGAC GCTCTGAGCG TGACTGCTCT
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACACCCTTG AGAGAGCCTT CCCACTTTCT
    TCTGTCTCTG TTAATGATTG CTCCACCAGT TTACGTGCCT TTGAAGATGA GCAGCCGTCA
    ACACTATCAC CCAAAAAAAA GCAACGAAAT GGAGGCATGC GAAATTCACC CAACTCCTCA
    CCCAAACTGA TGAGGCATGA CCCCTTGTTA ATTCCTGGCA ATGAACAGAT TGACAATATG
    GATGCCAATG TGAAAAAATA TGACTCTACG GGAATGTTTC ATTGGTGTCC AGCCAAGGAT
    ATTGAAAAGG TCATTTTGAC TCGAAGTGAA GCAGCGATGA CAGTTTTAAG TGGGCATGTA
    GTCGTTTGCA TATTTGGGGA TGTTAAATCT GCTTTGATTG GATTAAGAAA TTTAGTGATG
    CCATTACGAG CCAGCAACTT TCACTACCAT GAACTCAAGC ACATTGTGTT TGTGGGTTCA
    CTTGAATACC TGAGAAGGGA ATGGGAGACC CTGCATAACT TCCCCAAGGT TTCAATCTTG
    CCTGGTACAC CATTAAGTCG GGCTGATTTA AGGGCTGTCA ACATCAACCT CTGCGACATG
    TGCGTTATCC TGTCAGCCAA TCAGAATAAT ATTGATGATG CTTCGCTGCA GGACAAGGAA
    TGCATCTTGG CGTCACTCAA CATCAAATCT ATGCAGTTTG ATGACAGCAT TGGGGTCTTG
    CAGGCTAATT CCCAAGGCTT TACACCCCCT GGTATGGATA GGTCATCTCC AGACAACAGT
    CCTGTCCACG GCCTGTTACG TCAACCCTCC ATTACAACAG GAGCCAACAT CCCTATTATA
    ACAGAGCTAG TAAATGATTC AAATGTTCAG TTCTTGGACC AAGATGATGA TGATGATCCA
    GATACAGAAC TGTACCTCAC GCAGCCCTTT GCATGTGGAA CCGCATTTGC TGTCAGCGTG
    CTGGACTCTC TCATGAGCGC AACATACTTC AATGACAATA TCCTCACACT GATACGGACA
    TTGGTAACAG GAGGAGCCAC ACCAGAGCTG GAAGCACTGA TTGCCGAGGA AAATGCTTTG
    AGAGGAGGTT ACAGCACGCC CCAGACACTT GCAAACAGGG ACAGGTGTCG AGTTGCTCAG
    TTGGCTTTGT ATGATGGGCC ATTTGCAGAC CTAGGGGATG GAGGCTGCTA TGGAGATCTA
    TTTTGTAAAG CACTGAAAAC ATACAATATG CTTTGCTTTG GAATTTATCG ATTAAGGGAC
    GCTCATCTAA GTACTCCCAG CCAATGCACT AAACGTTATG TTATCACAAA CCCACCATAT
    GAGTTTGAGC TTGTGCCGAC AGACTTGATC TTCTGTTTGA TGCAATTCGA CCACAATGCT
    GGCCAGTCCC GGGCCAGTCT TTCTCATTCC TCCCATTCCT CCTATTCTTC CAGCAAGAAG
    AGCTCATCAG TTCACTCCAT CCCATCAACA GCAAACCGAC CGAACCGCAC CAAGACCAGG
    GATTCCCGGG AAAAACAGAA GTATGTGCAG GAAGATAGAC TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143656.1
    CDS116..3760
    Translation

    Target ORF information:

    RefSeq Version XM_015288170.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288170.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCCCAA GATGTCCATC
    TACAAGAGAA TGAAACTGGC ATGTTGTTTT GATTGCGGAC GCTCTGAGCG TGACTGCTCT
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACACCCTTG AGAGAGCCTT CCCACTTTCT
    TCTGTCTCTG TTAATGATTG CTCCACCAGT TTACGTGCCT TTGAAGATGA GCAGCCGTCA
    ACACTATCAC CCAAAAAAAA GCAACGAAAT GGAGGCATGC GAAATTCACC CAACTCCTCA
    CCCAAACTGA TGAGGCATGA CCCCTTGTTA ATTCCTGGCA ATGAACAGAT TGACAATATG
    GATGCCAATG TGAAAAAATA TGACTCTACG GGAATGTTTC ATTGGTGTCC AGCCAAGGAT
    ATTGAAAAGG TCATTTTGAC TCGAAGTGAA GCAGCGATGA CAGTTTTAAG TGGGCATGTA
    GTCGTTTGCA TATTTGGGGA TGTTAAATCT GCTTTGATTG GATTAAGAAA TTTAGTGATG
    CCATTACGAG CCAGCAACTT TCACTACCAT GAACTCAAGC ACATTGTGTT TGTGGGTTCA
    CTTGAATACC TGAGAAGGGA ATGGGAGACC CTGCATAACT TCCCCAAGGT TTCAATCTTG
    CCTGGTACAC CATTAAGTCG GGCTGATTTA AGGGCTGTCA ACATCAACCT CTGCGACATG
    TGCGTTATCC TGTCAGCCAA TCAGAATAAT ATTGATGATG CTTCGCTGCA GGACAAGGAA
    TGCATCTTGG CGTCACTCAA CATCAAATCT ATGCAGTTTG ATGACAGCAT TGGGGTCTTG
    CAGGCTAATT CCCAAGGCTT TACACCCCCT GGTATGGATA GGTCATCTCC AGACAACAGT
    CCTGTCCACG GCCTGTTACG TCAACCCTCC ATTACAACAG GAGCCAACAT CCCTATTATA
    ACAGAGCTAG TAAATGATTC AAATGTTCAG TTCTTGGACC AAGATGATGA TGATGATCCA
    GATACAGAAC TGTACCTCAC GCAGCCCTTT GCATGTGGAA CCGCATTTGC TGTCAGCGTG
    CTGGACTCTC TCATGAGCGC AACATACTTC AATGACAATA TCCTCACACT GATACGGACA
    TTGGTAACAG GAGGAGCCAC ACCAGAGCTG GAAGCACTGA TTGCCGAGGA AAATGCTTTG
    AGAGGAGGTT ACAGCACGCC CCAGACACTT GCAAACAGGG ACAGGTGTCG AGTTGCTCAG
    TTGGCTTTGT ATGATGGGCC ATTTGCAGAC CTAGGGGATG GAGGCTGCTA TGGAGATCTA
    TTTTGTAAAG CACTGAAAAC ATACAATATG CTTTGCTTTG GAATTTATCG ATTAAGGGAC
    GCTCATCTAA GTACTCCCAG CCAATGCACT AAACGTTATG TTATCACAAA CCCACCATAT
    GAGTTTGAGC TTGTGCCGAC AGACTTGATC TTCTGTTTGA TGCAATTCGA CCACAATGCT
    GGCCAGTCCC GGGCCAGTCT TTCTCATTCC TCCCATTCCT CCTATTCTTC CAGCAAGAAG
    AGCTCATCAG TTCACTCCAT CCCATCAACA GCAAACCGAC CGAACCGCAC CAAGACCAGG
    GATTCCCGGG AAAAACAGAA GTATGTGCAG GAAGATAGAC TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37444
    Clone ID Related Accession (Same CDS sequence) XM_015288171.2 , XM_015288171.1
    Accession Version XM_015288171.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3645bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288171.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCCCAA GATGTCCATC
    TACAAGAGAA TGAAACTGGC ATGTTGTTTT GATTGCGGAC GCTCTGAGCG TGACTGCTCT
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACACCCTTG AGAGAGCCTT CCCACTTTCT
    TCTGTCTCTG TTAATGATTG CTCCACCAGT TTACGTGCCT TTGAAGATGA GCAGCCGTCA
    ACACTATCAC CCAAAAAAAA GCAACGAAAT GGAGGCATGC GAAATTCACC CAACTCCTCA
    CCCAAACTGA TGAGGCATGA CCCCTTGTTA ATTCCTGGCA ATGAACAGAT TGACAATATG
    GATGCCAATG TGAAAAAATA TGACTCTACG GGAATGTTTC ATTGGTGTCC AGCCAAGGAT
    ATTGAAAAGG TCATTTTGAC TCGAAGTGAA GCAGCGATGA CAGTTTTAAG TGGGCATGTA
    GTCGTTTGCA TATTTGGGGA TGTTAAATCT GCTTTGATTG GATTAAGAAA TTTAGTGATG
    CCATTACGAG CCAGCAACTT TCACTACCAT GAACTCAAGC ACATTGTGTT TGTGGGTTCA
    CTTGAATACC TGAGAAGGGA ATGGGAGACC CTGCATAACT TCCCCAAGGT TTCAATCTTG
    CCTGGTACAC CATTAAGTCG GGCTGATTTA AGGGCTGTCA ACATCAACCT CTGCGACATG
    TGCGTTATCC TGTCAGCCAA TCAGAATAAT ATTGATGATG CTTCGCTGCA GGACAAGGAA
    TGCATCTTGG CGTCACTCAA CATCAAATCT ATGCAGTTTG ATGACAGCAT TGGGGTCTTG
    CAGGCTAATT CCCAAGGCTT TACACCCCCT GGTATGGATA GGTCATCTCC AGACAACAGT
    CCTGTCCACG GCCTGTTACG TCAACCCTCC ATTACAACAG GAGCCAACAT CCCTATTATA
    ACAGAGCTAG TAAATGATTC AAATGTTCAG TTCTTGGACC AAGATGATGA TGATGATCCA
    GATACAGAAC TGTACCTCAC GCAGCCCTTT GCATGTGGAA CCGCATTTGC TGTCAGCGTG
    CTGGACTCTC TCATGAGCGC AACATACTTC AATGACAATA TCCTCACACT GATACGGACA
    TTGGTAACAG GAGGAGCCAC ACCAGAGCTG GAAGCACTGA TTGCCGAGGA AAATGCTTTG
    AGAGGAGGTT ACAGCACGCC CCAGACACTT GCAAACAGGG ACAGGTGTCG AGTTGCTCAG
    TTGGCTTTGT ATGATGGGCC ATTTGCAGAC CTAGGGGATG GAGGCTGCTA TGGAGATCTA
    TTTTGTAAAG CACTGAAAAC ATACAATATG CTTTGCTTTG GAATTTATCG ATTAAGGGAC
    GCTCATCTAA GTACTCCCAG CCAATGCACT AAACGTTATG TTATCACAAA CCCACCATAT
    GAGTTTGAGC TTGTGCCGAC AGACTTGATC TTCTGTTTGA TGCAATTCGA CCACAATGCT
    GGCCAGTCCC GGGCCAGTCT TTCTCATTCC TCCCATTCCT CCTATTCTTC CAGCAAGAAG
    AGCTCATCAG TTCACTCCAT CCCATCAACA GCAAACCGAC CGAACCGCAC CAAGACCAGG
    GATTCCCGGG AAAAACAGAA CAGAAAAGAA ATGGTTTACA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143657.1
    CDS367..4011
    Translation

    Target ORF information:

    RefSeq Version XM_015288171.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288171.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCCCAA GATGTCCATC
    TACAAGAGAA TGAAACTGGC ATGTTGTTTT GATTGCGGAC GCTCTGAGCG TGACTGCTCT
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACACCCTTG AGAGAGCCTT CCCACTTTCT
    TCTGTCTCTG TTAATGATTG CTCCACCAGT TTACGTGCCT TTGAAGATGA GCAGCCGTCA
    ACACTATCAC CCAAAAAAAA GCAACGAAAT GGAGGCATGC GAAATTCACC CAACTCCTCA
    CCCAAACTGA TGAGGCATGA CCCCTTGTTA ATTCCTGGCA ATGAACAGAT TGACAATATG
    GATGCCAATG TGAAAAAATA TGACTCTACG GGAATGTTTC ATTGGTGTCC AGCCAAGGAT
    ATTGAAAAGG TCATTTTGAC TCGAAGTGAA GCAGCGATGA CAGTTTTAAG TGGGCATGTA
    GTCGTTTGCA TATTTGGGGA TGTTAAATCT GCTTTGATTG GATTAAGAAA TTTAGTGATG
    CCATTACGAG CCAGCAACTT TCACTACCAT GAACTCAAGC ACATTGTGTT TGTGGGTTCA
    CTTGAATACC TGAGAAGGGA ATGGGAGACC CTGCATAACT TCCCCAAGGT TTCAATCTTG
    CCTGGTACAC CATTAAGTCG GGCTGATTTA AGGGCTGTCA ACATCAACCT CTGCGACATG
    TGCGTTATCC TGTCAGCCAA TCAGAATAAT ATTGATGATG CTTCGCTGCA GGACAAGGAA
    TGCATCTTGG CGTCACTCAA CATCAAATCT ATGCAGTTTG ATGACAGCAT TGGGGTCTTG
    CAGGCTAATT CCCAAGGCTT TACACCCCCT GGTATGGATA GGTCATCTCC AGACAACAGT
    CCTGTCCACG GCCTGTTACG TCAACCCTCC ATTACAACAG GAGCCAACAT CCCTATTATA
    ACAGAGCTAG TAAATGATTC AAATGTTCAG TTCTTGGACC AAGATGATGA TGATGATCCA
    GATACAGAAC TGTACCTCAC GCAGCCCTTT GCATGTGGAA CCGCATTTGC TGTCAGCGTG
    CTGGACTCTC TCATGAGCGC AACATACTTC AATGACAATA TCCTCACACT GATACGGACA
    TTGGTAACAG GAGGAGCCAC ACCAGAGCTG GAAGCACTGA TTGCCGAGGA AAATGCTTTG
    AGAGGAGGTT ACAGCACGCC CCAGACACTT GCAAACAGGG ACAGGTGTCG AGTTGCTCAG
    TTGGCTTTGT ATGATGGGCC ATTTGCAGAC CTAGGGGATG GAGGCTGCTA TGGAGATCTA
    TTTTGTAAAG CACTGAAAAC ATACAATATG CTTTGCTTTG GAATTTATCG ATTAAGGGAC
    GCTCATCTAA GTACTCCCAG CCAATGCACT AAACGTTATG TTATCACAAA CCCACCATAT
    GAGTTTGAGC TTGTGCCGAC AGACTTGATC TTCTGTTTGA TGCAATTCGA CCACAATGCT
    GGCCAGTCCC GGGCCAGTCT TTCTCATTCC TCCCATTCCT CCTATTCTTC CAGCAAGAAG
    AGCTCATCAG TTCACTCCAT CCCATCAACA GCAAACCGAC CGAACCGCAC CAAGACCAGG
    GATTCCCGGG AAAAACAGAA CAGAAAAGAA ATGGTTTACA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37444
    Clone ID Related Accession (Same CDS sequence) XM_015288171.2 , XM_015288171.1
    Accession Version XM_015288171.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3645bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCCCAA GATGTCCATC
    TACAAGAGAA TGAAACTGGC ATGTTGTTTT GATTGCGGAC GCTCTGAGCG TGACTGCTCT
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACACCCTTG AGAGAGCCTT CCCACTTTCT
    TCTGTCTCTG TTAATGATTG CTCCACCAGT TTACGTGCCT TTGAAGATGA GCAGCCGTCA
    ACACTATCAC CCAAAAAAAA GCAACGAAAT GGAGGCATGC GAAATTCACC CAACTCCTCA
    CCCAAACTGA TGAGGCATGA CCCCTTGTTA ATTCCTGGCA ATGAACAGAT TGACAATATG
    GATGCCAATG TGAAAAAATA TGACTCTACG GGAATGTTTC ATTGGTGTCC AGCCAAGGAT
    ATTGAAAAGG TCATTTTGAC TCGAAGTGAA GCAGCGATGA CAGTTTTAAG TGGGCATGTA
    GTCGTTTGCA TATTTGGGGA TGTTAAATCT GCTTTGATTG GATTAAGAAA TTTAGTGATG
    CCATTACGAG CCAGCAACTT TCACTACCAT GAACTCAAGC ACATTGTGTT TGTGGGTTCA
    CTTGAATACC TGAGAAGGGA ATGGGAGACC CTGCATAACT TCCCCAAGGT TTCAATCTTG
    CCTGGTACAC CATTAAGTCG GGCTGATTTA AGGGCTGTCA ACATCAACCT CTGCGACATG
    TGCGTTATCC TGTCAGCCAA TCAGAATAAT ATTGATGATG CTTCGCTGCA GGACAAGGAA
    TGCATCTTGG CGTCACTCAA CATCAAATCT ATGCAGTTTG ATGACAGCAT TGGGGTCTTG
    CAGGCTAATT CCCAAGGCTT TACACCCCCT GGTATGGATA GGTCATCTCC AGACAACAGT
    CCTGTCCACG GCCTGTTACG TCAACCCTCC ATTACAACAG GAGCCAACAT CCCTATTATA
    ACAGAGCTAG TAAATGATTC AAATGTTCAG TTCTTGGACC AAGATGATGA TGATGATCCA
    GATACAGAAC TGTACCTCAC GCAGCCCTTT GCATGTGGAA CCGCATTTGC TGTCAGCGTG
    CTGGACTCTC TCATGAGCGC AACATACTTC AATGACAATA TCCTCACACT GATACGGACA
    TTGGTAACAG GAGGAGCCAC ACCAGAGCTG GAAGCACTGA TTGCCGAGGA AAATGCTTTG
    AGAGGAGGTT ACAGCACGCC CCAGACACTT GCAAACAGGG ACAGGTGTCG AGTTGCTCAG
    TTGGCTTTGT ATGATGGGCC ATTTGCAGAC CTAGGGGATG GAGGCTGCTA TGGAGATCTA
    TTTTGTAAAG CACTGAAAAC ATACAATATG CTTTGCTTTG GAATTTATCG ATTAAGGGAC
    GCTCATCTAA GTACTCCCAG CCAATGCACT AAACGTTATG TTATCACAAA CCCACCATAT
    GAGTTTGAGC TTGTGCCGAC AGACTTGATC TTCTGTTTGA TGCAATTCGA CCACAATGCT
    GGCCAGTCCC GGGCCAGTCT TTCTCATTCC TCCCATTCCT CCTATTCTTC CAGCAAGAAG
    AGCTCATCAG TTCACTCCAT CCCATCAACA GCAAACCGAC CGAACCGCAC CAAGACCAGG
    GATTCCCGGG AAAAACAGAA CAGAAAAGAA ATGGTTTACA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143657.1
    CDS373..4017
    Translation

    Target ORF information:

    RefSeq Version XM_015288171.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288171.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCCCAA GATGTCCATC
    TACAAGAGAA TGAAACTGGC ATGTTGTTTT GATTGCGGAC GCTCTGAGCG TGACTGCTCT
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACACCCTTG AGAGAGCCTT CCCACTTTCT
    TCTGTCTCTG TTAATGATTG CTCCACCAGT TTACGTGCCT TTGAAGATGA GCAGCCGTCA
    ACACTATCAC CCAAAAAAAA GCAACGAAAT GGAGGCATGC GAAATTCACC CAACTCCTCA
    CCCAAACTGA TGAGGCATGA CCCCTTGTTA ATTCCTGGCA ATGAACAGAT TGACAATATG
    GATGCCAATG TGAAAAAATA TGACTCTACG GGAATGTTTC ATTGGTGTCC AGCCAAGGAT
    ATTGAAAAGG TCATTTTGAC TCGAAGTGAA GCAGCGATGA CAGTTTTAAG TGGGCATGTA
    GTCGTTTGCA TATTTGGGGA TGTTAAATCT GCTTTGATTG GATTAAGAAA TTTAGTGATG
    CCATTACGAG CCAGCAACTT TCACTACCAT GAACTCAAGC ACATTGTGTT TGTGGGTTCA
    CTTGAATACC TGAGAAGGGA ATGGGAGACC CTGCATAACT TCCCCAAGGT TTCAATCTTG
    CCTGGTACAC CATTAAGTCG GGCTGATTTA AGGGCTGTCA ACATCAACCT CTGCGACATG
    TGCGTTATCC TGTCAGCCAA TCAGAATAAT ATTGATGATG CTTCGCTGCA GGACAAGGAA
    TGCATCTTGG CGTCACTCAA CATCAAATCT ATGCAGTTTG ATGACAGCAT TGGGGTCTTG
    CAGGCTAATT CCCAAGGCTT TACACCCCCT GGTATGGATA GGTCATCTCC AGACAACAGT
    CCTGTCCACG GCCTGTTACG TCAACCCTCC ATTACAACAG GAGCCAACAT CCCTATTATA
    ACAGAGCTAG TAAATGATTC AAATGTTCAG TTCTTGGACC AAGATGATGA TGATGATCCA
    GATACAGAAC TGTACCTCAC GCAGCCCTTT GCATGTGGAA CCGCATTTGC TGTCAGCGTG
    CTGGACTCTC TCATGAGCGC AACATACTTC AATGACAATA TCCTCACACT GATACGGACA
    TTGGTAACAG GAGGAGCCAC ACCAGAGCTG GAAGCACTGA TTGCCGAGGA AAATGCTTTG
    AGAGGAGGTT ACAGCACGCC CCAGACACTT GCAAACAGGG ACAGGTGTCG AGTTGCTCAG
    TTGGCTTTGT ATGATGGGCC ATTTGCAGAC CTAGGGGATG GAGGCTGCTA TGGAGATCTA
    TTTTGTAAAG CACTGAAAAC ATACAATATG CTTTGCTTTG GAATTTATCG ATTAAGGGAC
    GCTCATCTAA GTACTCCCAG CCAATGCACT AAACGTTATG TTATCACAAA CCCACCATAT
    GAGTTTGAGC TTGTGCCGAC AGACTTGATC TTCTGTTTGA TGCAATTCGA CCACAATGCT
    GGCCAGTCCC GGGCCAGTCT TTCTCATTCC TCCCATTCCT CCTATTCTTC CAGCAAGAAG
    AGCTCATCAG TTCACTCCAT CCCATCAACA GCAAACCGAC CGAACCGCAC CAAGACCAGG
    GATTCCCGGG AAAAACAGAA CAGAAAAGAA ATGGTTTACA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37445
    Clone ID Related Accession (Same CDS sequence) XM_015288172.1 , XM_015288172.2
    Accession Version XM_015288172.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCCCAA GATGTCCATC
    TACAAGAGAA TGAAACTGGC ATGTTGTTTT GATTGCGGAC GCTCTGAGCG TGACTGCTCT
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACACCCTTG AGAGAGCCTT CCCACTTTCT
    TCTGTCTCTG TTAATGATTG CTCCACCAGT TTACGTGCCT TTGAAGATGA GCAGCCGTCA
    ACACTATCAC CCAAAAAAAA GCAACGAAAT GGAGGCATGC GAAATTCACC CAACTCCTCA
    CCCAAACTGA TGAGGCATGA CCCCTTGTTA ATTCCTGGCA ATGAACAGAT TGACAATATG
    GATGCCAATG TGAAAAAATA TGACTCTACG GGAATGTTTC ATTGGTGTCC AGCCAAGGAT
    ATTGAAAAGG TCATTTTGAC TCGAAGTGAA GCAGCGATGA CAGTTTTAAG TGGGCATGTA
    GTCGTTTGCA TATTTGGGGA TGTTAAATCT GCTTTGATTG GATTAAGAAA TTTAGTGATG
    CCATTACGAG CCAGCAACTT TCACTACCAT GAACTCAAGC ACATTGTGTT TGTGGGTTCA
    CTTGAATACC TGAGAAGGGA ATGGGAGACC CTGCATAACT TCCCCAAGGT TTCAATCTTG
    CCTGGTACAC CATTAAGTCG GGCTGATTTA AGGGCTGTCA ACATCAACCT CTGCGACATG
    TGCGTTATCC TGTCAGCCAA TCAGAATAAT ATTGATGATG CTTCGCTGCA GGACAAGGAA
    TGCATCTTGG CGTCACTCAA CATCAAATCT ATGCAGTTTG ATGACAGCAT TGGGGTCTTG
    CAGGCTAATT CCCAAGGCTT TACACCCCCT GGTATGGATA GGTCATCTCC AGACAACAGT
    CCTGTCCACG GCCTGTTACG TCAACCCTCC ATTACAACAG GAGCCAACAT CCCTATTATA
    ACAGAGCTAG TAAATGATTC AAATGTTCAG TTCTTGGACC AAGATGATGA TGATGATCCA
    GATACAGAAC TGTACCTCAC GCAGCCCTTT GCATGTGGAA CCGCATTTGC TGTCAGCGTG
    CTGGACTCTC TCATGAGCGC AACATACTTC AATGACAATA TCCTCACACT GATACGGACA
    TTGGTAACAG GAGGAGCCAC ACCAGAGCTG GAAGCACTGA TTGCCGAGGA AAATGCTTTG
    AGAGGAGGTT ACAGCACGCC CCAGACACTT GCAAACAGGG ACAGGTGTCG AGTTGCTCAG
    TTGGCTTTGT ATGATGGGCC ATTTGCAGAC CTAGGGGATG GAGGCTGCTA TGGAGATCTA
    TTTTGTAAAG CACTGAAAAC ATACAATATG CTTTGCTTTG GAATTTATCG ATTAAGGGAC
    GCTCATCTAA GTACTCCCAG CCAATGCACT AAACGTTATG TTATCACAAA CCCACCATAT
    GAGTTTGAGC TTGTGCCGAC AGACTTGATC TTCTGTTTGA TGCAATTCGA CCACAATGCT
    GGCCAGTCCC GGGCCAGTCT TTCTCATTCC TCCCATTCCT CCTATTCTTC CAGCAAGAAG
    AGCTCATCAG TTCACTCCAT CCCATCAACA GCAAACCGAC CGAACCGCAC CAAGACCAGG
    GATTCCCGGG AAAAACAGAA AAAAGAAATG GTTTACAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143658.1
    CDS373..4014
    Translation

    Target ORF information:

    RefSeq Version XM_015288172.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288172.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCCCAA GATGTCCATC
    TACAAGAGAA TGAAACTGGC ATGTTGTTTT GATTGCGGAC GCTCTGAGCG TGACTGCTCT
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACACCCTTG AGAGAGCCTT CCCACTTTCT
    TCTGTCTCTG TTAATGATTG CTCCACCAGT TTACGTGCCT TTGAAGATGA GCAGCCGTCA
    ACACTATCAC CCAAAAAAAA GCAACGAAAT GGAGGCATGC GAAATTCACC CAACTCCTCA
    CCCAAACTGA TGAGGCATGA CCCCTTGTTA ATTCCTGGCA ATGAACAGAT TGACAATATG
    GATGCCAATG TGAAAAAATA TGACTCTACG GGAATGTTTC ATTGGTGTCC AGCCAAGGAT
    ATTGAAAAGG TCATTTTGAC TCGAAGTGAA GCAGCGATGA CAGTTTTAAG TGGGCATGTA
    GTCGTTTGCA TATTTGGGGA TGTTAAATCT GCTTTGATTG GATTAAGAAA TTTAGTGATG
    CCATTACGAG CCAGCAACTT TCACTACCAT GAACTCAAGC ACATTGTGTT TGTGGGTTCA
    CTTGAATACC TGAGAAGGGA ATGGGAGACC CTGCATAACT TCCCCAAGGT TTCAATCTTG
    CCTGGTACAC CATTAAGTCG GGCTGATTTA AGGGCTGTCA ACATCAACCT CTGCGACATG
    TGCGTTATCC TGTCAGCCAA TCAGAATAAT ATTGATGATG CTTCGCTGCA GGACAAGGAA
    TGCATCTTGG CGTCACTCAA CATCAAATCT ATGCAGTTTG ATGACAGCAT TGGGGTCTTG
    CAGGCTAATT CCCAAGGCTT TACACCCCCT GGTATGGATA GGTCATCTCC AGACAACAGT
    CCTGTCCACG GCCTGTTACG TCAACCCTCC ATTACAACAG GAGCCAACAT CCCTATTATA
    ACAGAGCTAG TAAATGATTC AAATGTTCAG TTCTTGGACC AAGATGATGA TGATGATCCA
    GATACAGAAC TGTACCTCAC GCAGCCCTTT GCATGTGGAA CCGCATTTGC TGTCAGCGTG
    CTGGACTCTC TCATGAGCGC AACATACTTC AATGACAATA TCCTCACACT GATACGGACA
    TTGGTAACAG GAGGAGCCAC ACCAGAGCTG GAAGCACTGA TTGCCGAGGA AAATGCTTTG
    AGAGGAGGTT ACAGCACGCC CCAGACACTT GCAAACAGGG ACAGGTGTCG AGTTGCTCAG
    TTGGCTTTGT ATGATGGGCC ATTTGCAGAC CTAGGGGATG GAGGCTGCTA TGGAGATCTA
    TTTTGTAAAG CACTGAAAAC ATACAATATG CTTTGCTTTG GAATTTATCG ATTAAGGGAC
    GCTCATCTAA GTACTCCCAG CCAATGCACT AAACGTTATG TTATCACAAA CCCACCATAT
    GAGTTTGAGC TTGTGCCGAC AGACTTGATC TTCTGTTTGA TGCAATTCGA CCACAATGCT
    GGCCAGTCCC GGGCCAGTCT TTCTCATTCC TCCCATTCCT CCTATTCTTC CAGCAAGAAG
    AGCTCATCAG TTCACTCCAT CCCATCAACA GCAAACCGAC CGAACCGCAC CAAGACCAGG
    GATTCCCGGG AAAAACAGAA AAAAGAAATG GTTTACAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37445
    Clone ID Related Accession (Same CDS sequence) XM_015288172.1 , XM_015288172.2
    Accession Version XM_015288172.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288172.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCCCAA GATGTCCATC
    TACAAGAGAA TGAAACTGGC ATGTTGTTTT GATTGCGGAC GCTCTGAGCG TGACTGCTCT
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACACCCTTG AGAGAGCCTT CCCACTTTCT
    TCTGTCTCTG TTAATGATTG CTCCACCAGT TTACGTGCCT TTGAAGATGA GCAGCCGTCA
    ACACTATCAC CCAAAAAAAA GCAACGAAAT GGAGGCATGC GAAATTCACC CAACTCCTCA
    CCCAAACTGA TGAGGCATGA CCCCTTGTTA ATTCCTGGCA ATGAACAGAT TGACAATATG
    GATGCCAATG TGAAAAAATA TGACTCTACG GGAATGTTTC ATTGGTGTCC AGCCAAGGAT
    ATTGAAAAGG TCATTTTGAC TCGAAGTGAA GCAGCGATGA CAGTTTTAAG TGGGCATGTA
    GTCGTTTGCA TATTTGGGGA TGTTAAATCT GCTTTGATTG GATTAAGAAA TTTAGTGATG
    CCATTACGAG CCAGCAACTT TCACTACCAT GAACTCAAGC ACATTGTGTT TGTGGGTTCA
    CTTGAATACC TGAGAAGGGA ATGGGAGACC CTGCATAACT TCCCCAAGGT TTCAATCTTG
    CCTGGTACAC CATTAAGTCG GGCTGATTTA AGGGCTGTCA ACATCAACCT CTGCGACATG
    TGCGTTATCC TGTCAGCCAA TCAGAATAAT ATTGATGATG CTTCGCTGCA GGACAAGGAA
    TGCATCTTGG CGTCACTCAA CATCAAATCT ATGCAGTTTG ATGACAGCAT TGGGGTCTTG
    CAGGCTAATT CCCAAGGCTT TACACCCCCT GGTATGGATA GGTCATCTCC AGACAACAGT
    CCTGTCCACG GCCTGTTACG TCAACCCTCC ATTACAACAG GAGCCAACAT CCCTATTATA
    ACAGAGCTAG TAAATGATTC AAATGTTCAG TTCTTGGACC AAGATGATGA TGATGATCCA
    GATACAGAAC TGTACCTCAC GCAGCCCTTT GCATGTGGAA CCGCATTTGC TGTCAGCGTG
    CTGGACTCTC TCATGAGCGC AACATACTTC AATGACAATA TCCTCACACT GATACGGACA
    TTGGTAACAG GAGGAGCCAC ACCAGAGCTG GAAGCACTGA TTGCCGAGGA AAATGCTTTG
    AGAGGAGGTT ACAGCACGCC CCAGACACTT GCAAACAGGG ACAGGTGTCG AGTTGCTCAG
    TTGGCTTTGT ATGATGGGCC ATTTGCAGAC CTAGGGGATG GAGGCTGCTA TGGAGATCTA
    TTTTGTAAAG CACTGAAAAC ATACAATATG CTTTGCTTTG GAATTTATCG ATTAAGGGAC
    GCTCATCTAA GTACTCCCAG CCAATGCACT AAACGTTATG TTATCACAAA CCCACCATAT
    GAGTTTGAGC TTGTGCCGAC AGACTTGATC TTCTGTTTGA TGCAATTCGA CCACAATGCT
    GGCCAGTCCC GGGCCAGTCT TTCTCATTCC TCCCATTCCT CCTATTCTTC CAGCAAGAAG
    AGCTCATCAG TTCACTCCAT CCCATCAACA GCAAACCGAC CGAACCGCAC CAAGACCAGG
    GATTCCCGGG AAAAACAGAA AAAAGAAATG GTTTACAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143658.1
    CDS107..3748
    Translation

    Target ORF information:

    RefSeq Version XM_015288172.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288172.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCCCAA GATGTCCATC
    TACAAGAGAA TGAAACTGGC ATGTTGTTTT GATTGCGGAC GCTCTGAGCG TGACTGCTCT
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACACCCTTG AGAGAGCCTT CCCACTTTCT
    TCTGTCTCTG TTAATGATTG CTCCACCAGT TTACGTGCCT TTGAAGATGA GCAGCCGTCA
    ACACTATCAC CCAAAAAAAA GCAACGAAAT GGAGGCATGC GAAATTCACC CAACTCCTCA
    CCCAAACTGA TGAGGCATGA CCCCTTGTTA ATTCCTGGCA ATGAACAGAT TGACAATATG
    GATGCCAATG TGAAAAAATA TGACTCTACG GGAATGTTTC ATTGGTGTCC AGCCAAGGAT
    ATTGAAAAGG TCATTTTGAC TCGAAGTGAA GCAGCGATGA CAGTTTTAAG TGGGCATGTA
    GTCGTTTGCA TATTTGGGGA TGTTAAATCT GCTTTGATTG GATTAAGAAA TTTAGTGATG
    CCATTACGAG CCAGCAACTT TCACTACCAT GAACTCAAGC ACATTGTGTT TGTGGGTTCA
    CTTGAATACC TGAGAAGGGA ATGGGAGACC CTGCATAACT TCCCCAAGGT TTCAATCTTG
    CCTGGTACAC CATTAAGTCG GGCTGATTTA AGGGCTGTCA ACATCAACCT CTGCGACATG
    TGCGTTATCC TGTCAGCCAA TCAGAATAAT ATTGATGATG CTTCGCTGCA GGACAAGGAA
    TGCATCTTGG CGTCACTCAA CATCAAATCT ATGCAGTTTG ATGACAGCAT TGGGGTCTTG
    CAGGCTAATT CCCAAGGCTT TACACCCCCT GGTATGGATA GGTCATCTCC AGACAACAGT
    CCTGTCCACG GCCTGTTACG TCAACCCTCC ATTACAACAG GAGCCAACAT CCCTATTATA
    ACAGAGCTAG TAAATGATTC AAATGTTCAG TTCTTGGACC AAGATGATGA TGATGATCCA
    GATACAGAAC TGTACCTCAC GCAGCCCTTT GCATGTGGAA CCGCATTTGC TGTCAGCGTG
    CTGGACTCTC TCATGAGCGC AACATACTTC AATGACAATA TCCTCACACT GATACGGACA
    TTGGTAACAG GAGGAGCCAC ACCAGAGCTG GAAGCACTGA TTGCCGAGGA AAATGCTTTG
    AGAGGAGGTT ACAGCACGCC CCAGACACTT GCAAACAGGG ACAGGTGTCG AGTTGCTCAG
    TTGGCTTTGT ATGATGGGCC ATTTGCAGAC CTAGGGGATG GAGGCTGCTA TGGAGATCTA
    TTTTGTAAAG CACTGAAAAC ATACAATATG CTTTGCTTTG GAATTTATCG ATTAAGGGAC
    GCTCATCTAA GTACTCCCAG CCAATGCACT AAACGTTATG TTATCACAAA CCCACCATAT
    GAGTTTGAGC TTGTGCCGAC AGACTTGATC TTCTGTTTGA TGCAATTCGA CCACAATGCT
    GGCCAGTCCC GGGCCAGTCT TTCTCATTCC TCCCATTCCT CCTATTCTTC CAGCAAGAAG
    AGCTCATCAG TTCACTCCAT CCCATCAACA GCAAACCGAC CGAACCGCAC CAAGACCAGG
    GATTCCCGGG AAAAACAGAA AAAAGAAATG GTTTACAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37446
    Clone ID Related Accession (Same CDS sequence) XM_015288173.1
    Accession Version XM_015288173.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3639bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X29
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTT
    GAAGATGAGC AGCCGTCAAC ACTATCACCC AAAAAAAAGC AACGAAATGG AGGCATGCGA
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAT
    GAACAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACGGG AATGTTTCAT
    TGGTGTCCAG CCAAGGATAT TGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACCCT GCATAACTTC
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGCTTTA CACCCCCTGG TATGGATAGG
    TCATCTCCAG ACAACAGTCC TGTCCACGGC CTGTTACGTC AACCCTCCAT TACAACAGGA
    GCCAACATCC CTATTATAAC AGAGCTAGTA AATGATTCAA ATGTTCAGTT CTTGGACCAA
    GATGATGATG ATGATCCAGA TACAGAACTG TACCTCACGC AGCCCTTTGC ATGTGGAACC
    GCATTTGCTG TCAGCGTGCT GGACTCTCTC ATGAGCGCAA CATACTTCAA TGACAATATC
    CTCACACTGA TACGGACATT GGTAACAGGA GGAGCCACAC CAGAGCTGGA AGCACTGATT
    GCCGAGGAAA ATGCTTTGAG AGGAGGTTAC AGCACGCCCC AGACACTTGC AAACAGGGAC
    AGGTGTCGAG TTGCTCAGTT GGCTTTGTAT GATGGGCCAT TTGCAGACCT AGGGGATGGA
    GGCTGCTATG GAGATCTATT TTGTAAAGCA CTGAAAACAT ACAATATGCT TTGCTTTGGA
    ATTTATCGAT TAAGGGACGC TCATCTAAGT ACTCCCAGCC AATGCACTAA ACGTTATGTT
    ATCACAAACC CACCATATGA GTTTGAGCTT GTGCCGACAG ACTTGATCTT CTGTTTGATG
    CAATTCGACC ACAATGCTGG CCAGTCCCGG GCCAGTCTTT CTCATTCCTC CCATTCCTCC
    TATTCTTCCA GCAAGAAGAG CTCATCAGTT CACTCCATCC CATCAACAGC AAACCGACCG
    AACCGCACCA AGACCAGGGA TTCCCGGGAA AAACAGAATG CAACAAGGAT GAATAGAATG
    GGCCAAGAAA AGAAATGGTT TACAGATGAA CCGGATAATG CCTATCCCAG AAACATTCAA
    ATCAAGCCCA TGAGCACCCA CATGGCCAAT CAGATCAATC AATACAAATC GACAAGCAGC
    TTGATACCAC CAATCAGAGA AGTTGAAGAT GAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143659.1
    CDS116..3754
    Translation

    Target ORF information:

    RefSeq Version XM_015288173.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X29, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288173.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTT
    GAAGATGAGC AGCCGTCAAC ACTATCACCC AAAAAAAAGC AACGAAATGG AGGCATGCGA
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAT
    GAACAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACGGG AATGTTTCAT
    TGGTGTCCAG CCAAGGATAT TGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACCCT GCATAACTTC
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGCTTTA CACCCCCTGG TATGGATAGG
    TCATCTCCAG ACAACAGTCC TGTCCACGGC CTGTTACGTC AACCCTCCAT TACAACAGGA
    GCCAACATCC CTATTATAAC AGAGCTAGTA AATGATTCAA ATGTTCAGTT CTTGGACCAA
    GATGATGATG ATGATCCAGA TACAGAACTG TACCTCACGC AGCCCTTTGC ATGTGGAACC
    GCATTTGCTG TCAGCGTGCT GGACTCTCTC ATGAGCGCAA CATACTTCAA TGACAATATC
    CTCACACTGA TACGGACATT GGTAACAGGA GGAGCCACAC CAGAGCTGGA AGCACTGATT
    GCCGAGGAAA ATGCTTTGAG AGGAGGTTAC AGCACGCCCC AGACACTTGC AAACAGGGAC
    AGGTGTCGAG TTGCTCAGTT GGCTTTGTAT GATGGGCCAT TTGCAGACCT AGGGGATGGA
    GGCTGCTATG GAGATCTATT TTGTAAAGCA CTGAAAACAT ACAATATGCT TTGCTTTGGA
    ATTTATCGAT TAAGGGACGC TCATCTAAGT ACTCCCAGCC AATGCACTAA ACGTTATGTT
    ATCACAAACC CACCATATGA GTTTGAGCTT GTGCCGACAG ACTTGATCTT CTGTTTGATG
    CAATTCGACC ACAATGCTGG CCAGTCCCGG GCCAGTCTTT CTCATTCCTC CCATTCCTCC
    TATTCTTCCA GCAAGAAGAG CTCATCAGTT CACTCCATCC CATCAACAGC AAACCGACCG
    AACCGCACCA AGACCAGGGA TTCCCGGGAA AAACAGAATG CAACAAGGAT GAATAGAATG
    GGCCAAGAAA AGAAATGGTT TACAGATGAA CCGGATAATG CCTATCCCAG AAACATTCAA
    ATCAAGCCCA TGAGCACCCA CATGGCCAAT CAGATCAATC AATACAAATC GACAAGCAGC
    TTGATACCAC CAATCAGAGA AGTTGAAGAT GAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37447
    Clone ID Related Accession (Same CDS sequence) XM_015288174.1 , XM_015288174.2
    Accession Version XM_015288174.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3639bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X28
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGTAAAT GATTCAAATG TTCAGTTCTT GGACCAAGAT
    GATGATGATG ATCCAGATAC AGAACTGTAC CTCACGCAGC CCTTTGCATG TGGAACCGCA
    TTTGCTGTCA GCGTGCTGGA CTCTCTCATG AGCGCAACAT ACTTCAATGA CAATATCCTC
    ACACTGATAC GGACATTGGT AACAGGAGGA GCCACACCAG AGCTGGAAGC ACTGATTGCC
    GAGGAAAATG CTTTGAGAGG AGGTTACAGC ACGCCCCAGA CACTTGCAAA CAGGGACAGG
    TGTCGAGTTG CTCAGTTGGC TTTGTATGAT GGGCCATTTG CAGACCTAGG GGATGGAGGC
    TGCTATGGAG ATCTATTTTG TAAAGCACTG AAAACATACA ATATGCTTTG CTTTGGAATT
    TATCGATTAA GGGACGCTCA TCTAAGTACT CCCAGCCAAT GCACTAAACG TTATGTTATC
    ACAAACCCAC CATATGAGTT TGAGCTTGTG CCGACAGACT TGATCTTCTG TTTGATGCAA
    TTCGACCACA ATGCTGGCCA GTCCCGGGCC AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT
    TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC TCCATCCCAT CAACAGCAAA CCGACCGAAC
    CGCACCAAGA CCAGGGATTC CCGGGAAAAA CAGAATGCAA CAAGGATGAA TAGAATGGGC
    CAAGCAGAAA AGAAATGGTT TACAGATGAA CCGGATAATG CCTATCCCAG AAACATTCAA
    ATCAAGCCCA TGAGCACCCA CATGGCCAAT CAGATCAATC AATACAAATC GACAAGCAGC
    TTGATACCAC CAATCAGAGA AGTTGAAGAT GAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143660.1
    CDS373..4011
    Translation

    Target ORF information:

    RefSeq Version XM_015288174.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288174.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGTAAAT GATTCAAATG TTCAGTTCTT GGACCAAGAT
    GATGATGATG ATCCAGATAC AGAACTGTAC CTCACGCAGC CCTTTGCATG TGGAACCGCA
    TTTGCTGTCA GCGTGCTGGA CTCTCTCATG AGCGCAACAT ACTTCAATGA CAATATCCTC
    ACACTGATAC GGACATTGGT AACAGGAGGA GCCACACCAG AGCTGGAAGC ACTGATTGCC
    GAGGAAAATG CTTTGAGAGG AGGTTACAGC ACGCCCCAGA CACTTGCAAA CAGGGACAGG
    TGTCGAGTTG CTCAGTTGGC TTTGTATGAT GGGCCATTTG CAGACCTAGG GGATGGAGGC
    TGCTATGGAG ATCTATTTTG TAAAGCACTG AAAACATACA ATATGCTTTG CTTTGGAATT
    TATCGATTAA GGGACGCTCA TCTAAGTACT CCCAGCCAAT GCACTAAACG TTATGTTATC
    ACAAACCCAC CATATGAGTT TGAGCTTGTG CCGACAGACT TGATCTTCTG TTTGATGCAA
    TTCGACCACA ATGCTGGCCA GTCCCGGGCC AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT
    TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC TCCATCCCAT CAACAGCAAA CCGACCGAAC
    CGCACCAAGA CCAGGGATTC CCGGGAAAAA CAGAATGCAA CAAGGATGAA TAGAATGGGC
    CAAGCAGAAA AGAAATGGTT TACAGATGAA CCGGATAATG CCTATCCCAG AAACATTCAA
    ATCAAGCCCA TGAGCACCCA CATGGCCAAT CAGATCAATC AATACAAATC GACAAGCAGC
    TTGATACCAC CAATCAGAGA AGTTGAAGAT GAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37447
    Clone ID Related Accession (Same CDS sequence) XM_015288174.1 , XM_015288174.2
    Accession Version XM_015288174.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3639bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X30
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288174.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGTAAAT GATTCAAATG TTCAGTTCTT GGACCAAGAT
    GATGATGATG ATCCAGATAC AGAACTGTAC CTCACGCAGC CCTTTGCATG TGGAACCGCA
    TTTGCTGTCA GCGTGCTGGA CTCTCTCATG AGCGCAACAT ACTTCAATGA CAATATCCTC
    ACACTGATAC GGACATTGGT AACAGGAGGA GCCACACCAG AGCTGGAAGC ACTGATTGCC
    GAGGAAAATG CTTTGAGAGG AGGTTACAGC ACGCCCCAGA CACTTGCAAA CAGGGACAGG
    TGTCGAGTTG CTCAGTTGGC TTTGTATGAT GGGCCATTTG CAGACCTAGG GGATGGAGGC
    TGCTATGGAG ATCTATTTTG TAAAGCACTG AAAACATACA ATATGCTTTG CTTTGGAATT
    TATCGATTAA GGGACGCTCA TCTAAGTACT CCCAGCCAAT GCACTAAACG TTATGTTATC
    ACAAACCCAC CATATGAGTT TGAGCTTGTG CCGACAGACT TGATCTTCTG TTTGATGCAA
    TTCGACCACA ATGCTGGCCA GTCCCGGGCC AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT
    TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC TCCATCCCAT CAACAGCAAA CCGACCGAAC
    CGCACCAAGA CCAGGGATTC CCGGGAAAAA CAGAATGCAA CAAGGATGAA TAGAATGGGC
    CAAGCAGAAA AGAAATGGTT TACAGATGAA CCGGATAATG CCTATCCCAG AAACATTCAA
    ATCAAGCCCA TGAGCACCCA CATGGCCAAT CAGATCAATC AATACAAATC GACAAGCAGC
    TTGATACCAC CAATCAGAGA AGTTGAAGAT GAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143660.1
    CDS367..4005
    Translation

    Target ORF information:

    RefSeq Version XM_015288174.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X30, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288174.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGTAAAT GATTCAAATG TTCAGTTCTT GGACCAAGAT
    GATGATGATG ATCCAGATAC AGAACTGTAC CTCACGCAGC CCTTTGCATG TGGAACCGCA
    TTTGCTGTCA GCGTGCTGGA CTCTCTCATG AGCGCAACAT ACTTCAATGA CAATATCCTC
    ACACTGATAC GGACATTGGT AACAGGAGGA GCCACACCAG AGCTGGAAGC ACTGATTGCC
    GAGGAAAATG CTTTGAGAGG AGGTTACAGC ACGCCCCAGA CACTTGCAAA CAGGGACAGG
    TGTCGAGTTG CTCAGTTGGC TTTGTATGAT GGGCCATTTG CAGACCTAGG GGATGGAGGC
    TGCTATGGAG ATCTATTTTG TAAAGCACTG AAAACATACA ATATGCTTTG CTTTGGAATT
    TATCGATTAA GGGACGCTCA TCTAAGTACT CCCAGCCAAT GCACTAAACG TTATGTTATC
    ACAAACCCAC CATATGAGTT TGAGCTTGTG CCGACAGACT TGATCTTCTG TTTGATGCAA
    TTCGACCACA ATGCTGGCCA GTCCCGGGCC AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT
    TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC TCCATCCCAT CAACAGCAAA CCGACCGAAC
    CGCACCAAGA CCAGGGATTC CCGGGAAAAA CAGAATGCAA CAAGGATGAA TAGAATGGGC
    CAAGCAGAAA AGAAATGGTT TACAGATGAA CCGGATAATG CCTATCCCAG AAACATTCAA
    ATCAAGCCCA TGAGCACCCA CATGGCCAAT CAGATCAATC AATACAAATC GACAAGCAGC
    TTGATACCAC CAATCAGAGA AGTTGAAGAT GAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37448
    Clone ID Related Accession (Same CDS sequence) XM_015288175.2 , XM_015288175.1
    Accession Version XM_015288175.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3639bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X31
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288175.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCAAT GTTTGCTCGC
    TACGTGCCAG AAATTGCTGC TCTCATCCTT AATCGGAAGA AATACGGCGG GACTTTTAAC
    TCAACCCGAG GCAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTT
    GAAGATGAGC AGCCGTCAAC ACTATCACCC AAAAAAAAGC AACGAAATGG AGGCATGCGA
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAT
    GAACAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACGGG AATGTTTCAT
    TGGTGTCCAG CCAAGGATAT TGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACCCT GCATAACTTC
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGCTTTA CACCCCCTGG TATGGATAGG
    TCATCTCCAG ACAACAGTCC TGTCCACGGC CTGTTACGTC AACCCTCCAT TACAACAGGA
    GCCAACATCC CTATTATAAC AGAGCTAGTA AATGATTCAA ATGTTCAGTT CTTGGACCAA
    GATGATGATG ATGATCCAGA TACAGAACTG TACCTCACGC AGCCCTTTGC ATGTGGAACC
    GCATTTGCTG TCAGCGTGCT GGACTCTCTC ATGAGCGCAA CATACTTCAA TGACAATATC
    CTCACACTGA TACGGACATT GGTAACAGGA GGAGCCACAC CAGAGCTGGA AGCACTGATT
    GCCGAGGAAA ATGCTTTGAG AGGAGGTTAC AGCACGCCCC AGACACTTGC AAACAGGGAC
    AGGTGTCGAG TTGCTCAGTT GGCTTTGTAT GATGGGCCAT TTGCAGACCT AGGGGATGGA
    GGCTGCTATG GAGATCTATT TTGTAAAGCA CTGAAAACAT ACAATATGCT TTGCTTTGGA
    ATTTATCGAT TAAGGGACGC TCATCTAAGT ACTCCCAGCC AATGCACTAA ACGTTATGTT
    ATCACAAACC CACCATATGA GTTTGAGCTT GTGCCGACAG ACTTGATCTT CTGTTTGATG
    CAATTCGACC ACAATGCTGG CCAGTCCCGG GCCAGTCTTT CTCATTCCTC CCATTCCTCC
    TATTCTTCCA GCAAGAAGAG CTCATCAGTT CACTCCATCC CATCAACAGC AAACCGACCG
    AACCGCACCA AGACCAGGGA TTCCCGGGAA AAACAGAATG CAACAAGGAT GAATAGAATG
    GGCCAAGAAA AGAAATGGTT TACAGATGAA CCGGATAATG CCTATCCCAG AAACATTCAA
    ATCAAGCCCA TGAGCACCCA CATGGCCAAT CAGATCAATC AATACAAATC GACAAGCAGC
    TTGATACCAC CAATCAGAGA AGTTGAAGAT GAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143661.1
    CDS368..4006
    Translation

    Target ORF information:

    RefSeq Version XM_015288175.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X31, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288175.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCAAT GTTTGCTCGC
    TACGTGCCAG AAATTGCTGC TCTCATCCTT AATCGGAAGA AATACGGCGG GACTTTTAAC
    TCAACCCGAG GCAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTT
    GAAGATGAGC AGCCGTCAAC ACTATCACCC AAAAAAAAGC AACGAAATGG AGGCATGCGA
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAT
    GAACAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACGGG AATGTTTCAT
    TGGTGTCCAG CCAAGGATAT TGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACCCT GCATAACTTC
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGCTTTA CACCCCCTGG TATGGATAGG
    TCATCTCCAG ACAACAGTCC TGTCCACGGC CTGTTACGTC AACCCTCCAT TACAACAGGA
    GCCAACATCC CTATTATAAC AGAGCTAGTA AATGATTCAA ATGTTCAGTT CTTGGACCAA
    GATGATGATG ATGATCCAGA TACAGAACTG TACCTCACGC AGCCCTTTGC ATGTGGAACC
    GCATTTGCTG TCAGCGTGCT GGACTCTCTC ATGAGCGCAA CATACTTCAA TGACAATATC
    CTCACACTGA TACGGACATT GGTAACAGGA GGAGCCACAC CAGAGCTGGA AGCACTGATT
    GCCGAGGAAA ATGCTTTGAG AGGAGGTTAC AGCACGCCCC AGACACTTGC AAACAGGGAC
    AGGTGTCGAG TTGCTCAGTT GGCTTTGTAT GATGGGCCAT TTGCAGACCT AGGGGATGGA
    GGCTGCTATG GAGATCTATT TTGTAAAGCA CTGAAAACAT ACAATATGCT TTGCTTTGGA
    ATTTATCGAT TAAGGGACGC TCATCTAAGT ACTCCCAGCC AATGCACTAA ACGTTATGTT
    ATCACAAACC CACCATATGA GTTTGAGCTT GTGCCGACAG ACTTGATCTT CTGTTTGATG
    CAATTCGACC ACAATGCTGG CCAGTCCCGG GCCAGTCTTT CTCATTCCTC CCATTCCTCC
    TATTCTTCCA GCAAGAAGAG CTCATCAGTT CACTCCATCC CATCAACAGC AAACCGACCG
    AACCGCACCA AGACCAGGGA TTCCCGGGAA AAACAGAATG CAACAAGGAT GAATAGAATG
    GGCCAAGAAA AGAAATGGTT TACAGATGAA CCGGATAATG CCTATCCCAG AAACATTCAA
    ATCAAGCCCA TGAGCACCCA CATGGCCAAT CAGATCAATC AATACAAATC GACAAGCAGC
    TTGATACCAC CAATCAGAGA AGTTGAAGAT GAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37448
    Clone ID Related Accession (Same CDS sequence) XM_015288175.2 , XM_015288175.1
    Accession Version XM_015288175.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3639bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X29
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCAAT GTTTGCTCGC
    TACGTGCCAG AAATTGCTGC TCTCATCCTT AATCGGAAGA AATACGGCGG GACTTTTAAC
    TCAACCCGAG GCAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTT
    GAAGATGAGC AGCCGTCAAC ACTATCACCC AAAAAAAAGC AACGAAATGG AGGCATGCGA
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAT
    GAACAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACGGG AATGTTTCAT
    TGGTGTCCAG CCAAGGATAT TGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACCCT GCATAACTTC
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGCTTTA CACCCCCTGG TATGGATAGG
    TCATCTCCAG ACAACAGTCC TGTCCACGGC CTGTTACGTC AACCCTCCAT TACAACAGGA
    GCCAACATCC CTATTATAAC AGAGCTAGTA AATGATTCAA ATGTTCAGTT CTTGGACCAA
    GATGATGATG ATGATCCAGA TACAGAACTG TACCTCACGC AGCCCTTTGC ATGTGGAACC
    GCATTTGCTG TCAGCGTGCT GGACTCTCTC ATGAGCGCAA CATACTTCAA TGACAATATC
    CTCACACTGA TACGGACATT GGTAACAGGA GGAGCCACAC CAGAGCTGGA AGCACTGATT
    GCCGAGGAAA ATGCTTTGAG AGGAGGTTAC AGCACGCCCC AGACACTTGC AAACAGGGAC
    AGGTGTCGAG TTGCTCAGTT GGCTTTGTAT GATGGGCCAT TTGCAGACCT AGGGGATGGA
    GGCTGCTATG GAGATCTATT TTGTAAAGCA CTGAAAACAT ACAATATGCT TTGCTTTGGA
    ATTTATCGAT TAAGGGACGC TCATCTAAGT ACTCCCAGCC AATGCACTAA ACGTTATGTT
    ATCACAAACC CACCATATGA GTTTGAGCTT GTGCCGACAG ACTTGATCTT CTGTTTGATG
    CAATTCGACC ACAATGCTGG CCAGTCCCGG GCCAGTCTTT CTCATTCCTC CCATTCCTCC
    TATTCTTCCA GCAAGAAGAG CTCATCAGTT CACTCCATCC CATCAACAGC AAACCGACCG
    AACCGCACCA AGACCAGGGA TTCCCGGGAA AAACAGAATG CAACAAGGAT GAATAGAATG
    GGCCAAGAAA AGAAATGGTT TACAGATGAA CCGGATAATG CCTATCCCAG AAACATTCAA
    ATCAAGCCCA TGAGCACCCA CATGGCCAAT CAGATCAATC AATACAAATC GACAAGCAGC
    TTGATACCAC CAATCAGAGA AGTTGAAGAT GAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143661.1
    CDS373..4011
    Translation

    Target ORF information:

    RefSeq Version XM_015288175.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X29, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288175.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCAAT GTTTGCTCGC
    TACGTGCCAG AAATTGCTGC TCTCATCCTT AATCGGAAGA AATACGGCGG GACTTTTAAC
    TCAACCCGAG GCAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTT
    GAAGATGAGC AGCCGTCAAC ACTATCACCC AAAAAAAAGC AACGAAATGG AGGCATGCGA
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAT
    GAACAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACGGG AATGTTTCAT
    TGGTGTCCAG CCAAGGATAT TGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACCCT GCATAACTTC
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGCTTTA CACCCCCTGG TATGGATAGG
    TCATCTCCAG ACAACAGTCC TGTCCACGGC CTGTTACGTC AACCCTCCAT TACAACAGGA
    GCCAACATCC CTATTATAAC AGAGCTAGTA AATGATTCAA ATGTTCAGTT CTTGGACCAA
    GATGATGATG ATGATCCAGA TACAGAACTG TACCTCACGC AGCCCTTTGC ATGTGGAACC
    GCATTTGCTG TCAGCGTGCT GGACTCTCTC ATGAGCGCAA CATACTTCAA TGACAATATC
    CTCACACTGA TACGGACATT GGTAACAGGA GGAGCCACAC CAGAGCTGGA AGCACTGATT
    GCCGAGGAAA ATGCTTTGAG AGGAGGTTAC AGCACGCCCC AGACACTTGC AAACAGGGAC
    AGGTGTCGAG TTGCTCAGTT GGCTTTGTAT GATGGGCCAT TTGCAGACCT AGGGGATGGA
    GGCTGCTATG GAGATCTATT TTGTAAAGCA CTGAAAACAT ACAATATGCT TTGCTTTGGA
    ATTTATCGAT TAAGGGACGC TCATCTAAGT ACTCCCAGCC AATGCACTAA ACGTTATGTT
    ATCACAAACC CACCATATGA GTTTGAGCTT GTGCCGACAG ACTTGATCTT CTGTTTGATG
    CAATTCGACC ACAATGCTGG CCAGTCCCGG GCCAGTCTTT CTCATTCCTC CCATTCCTCC
    TATTCTTCCA GCAAGAAGAG CTCATCAGTT CACTCCATCC CATCAACAGC AAACCGACCG
    AACCGCACCA AGACCAGGGA TTCCCGGGAA AAACAGAATG CAACAAGGAT GAATAGAATG
    GGCCAAGAAA AGAAATGGTT TACAGATGAA CCGGATAATG CCTATCCCAG AAACATTCAA
    ATCAAGCCCA TGAGCACCCA CATGGCCAAT CAGATCAATC AATACAAATC GACAAGCAGC
    TTGATACCAC CAATCAGAGA AGTTGAAGAT GAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37449
    Clone ID Related Accession (Same CDS sequence) XM_015288176.1
    Accession Version XM_015288176.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X32
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGTAAAT GATTCAAATG TTCAGTTCTT GGACCAAGAT
    GATGATGATG ATCCAGATAC AGAACTGTAC CTCACGCAGC CCTTTGCATG TGGAACCGCA
    TTTGCTGTCA GCGTGCTGGA CTCTCTCATG AGCGCAACAT ACTTCAATGA CAATATCCTC
    ACACTGATAC GGACATTGGT AACAGGAGGA GCCACACCAG AGCTGGAAGC ACTGATTGCC
    GAGGAAAATG CTTTGAGAGG AGGTTACAGC ACGCCCCAGA CACTTGCAAA CAGGGACAGG
    TGTCGAGTTG CTCAGTTGGC TTTGTATGAT GGGCCATTTG CAGACCTAGG GGATGGAGGC
    TGCTATGGAG ATCTATTTTG TAAAGCACTG AAAACATACA ATATGCTTTG CTTTGGAATT
    TATCGATTAA GGGACGCTCA TCTAAGTACT CCCAGCCAAT GCACTAAACG TTATGTTATC
    ACAAACCCAC CATATGAGTT TGAGCTTGTG CCGACAGACT TGATCTTCTG TTTGATGCAA
    TTCGACCACA ATGCTGGCCA GTCCCGGGCC AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT
    TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC TCCATCCCAT CAACAGCAAA CCGACCGAAC
    CGCACCAAGA CCAGGGATTC CCGGGAAAAA CAGAATGCAA CAAGGATGAA TAGAATGGGC
    CAAGAAAAGA AATGGTTTAC AGATGAACCG GATAATGCCT ATCCCAGAAA CATTCAAATC
    AAGCCCATGA GCACCCACAT GGCCAATCAG ATCAATCAAT ACAAATCGAC AAGCAGCTTG
    ATACCACCAA TCAGAGAAGT TGAAGATGAA TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143662.1
    CDS116..3751
    Translation

    Target ORF information:

    RefSeq Version XM_015288176.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X32, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288176.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGTAAAT GATTCAAATG TTCAGTTCTT GGACCAAGAT
    GATGATGATG ATCCAGATAC AGAACTGTAC CTCACGCAGC CCTTTGCATG TGGAACCGCA
    TTTGCTGTCA GCGTGCTGGA CTCTCTCATG AGCGCAACAT ACTTCAATGA CAATATCCTC
    ACACTGATAC GGACATTGGT AACAGGAGGA GCCACACCAG AGCTGGAAGC ACTGATTGCC
    GAGGAAAATG CTTTGAGAGG AGGTTACAGC ACGCCCCAGA CACTTGCAAA CAGGGACAGG
    TGTCGAGTTG CTCAGTTGGC TTTGTATGAT GGGCCATTTG CAGACCTAGG GGATGGAGGC
    TGCTATGGAG ATCTATTTTG TAAAGCACTG AAAACATACA ATATGCTTTG CTTTGGAATT
    TATCGATTAA GGGACGCTCA TCTAAGTACT CCCAGCCAAT GCACTAAACG TTATGTTATC
    ACAAACCCAC CATATGAGTT TGAGCTTGTG CCGACAGACT TGATCTTCTG TTTGATGCAA
    TTCGACCACA ATGCTGGCCA GTCCCGGGCC AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT
    TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC TCCATCCCAT CAACAGCAAA CCGACCGAAC
    CGCACCAAGA CCAGGGATTC CCGGGAAAAA CAGAATGCAA CAAGGATGAA TAGAATGGGC
    CAAGAAAAGA AATGGTTTAC AGATGAACCG GATAATGCCT ATCCCAGAAA CATTCAAATC
    AAGCCCATGA GCACCCACAT GGCCAATCAG ATCAATCAAT ACAAATCGAC AAGCAGCTTG
    ATACCACCAA TCAGAGAAGT TGAAGATGAA TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37450
    Clone ID Related Accession (Same CDS sequence) XM_015288177.2 , XM_015288177.1
    Accession Version XM_015288177.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X33
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288177.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCAAT GTTTGCTCGC
    TACGTGCCAG AAATTGCTGC TCTCATCCTT AATCGGAAGA AATACGGCGG GACTTTTAAC
    TCAACCCGAG GCAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGTAAAT GATTCAAATG TTCAGTTCTT GGACCAAGAT
    GATGATGATG ATCCAGATAC AGAACTGTAC CTCACGCAGC CCTTTGCATG TGGAACCGCA
    TTTGCTGTCA GCGTGCTGGA CTCTCTCATG AGCGCAACAT ACTTCAATGA CAATATCCTC
    ACACTGATAC GGACATTGGT AACAGGAGGA GCCACACCAG AGCTGGAAGC ACTGATTGCC
    GAGGAAAATG CTTTGAGAGG AGGTTACAGC ACGCCCCAGA CACTTGCAAA CAGGGACAGG
    TGTCGAGTTG CTCAGTTGGC TTTGTATGAT GGGCCATTTG CAGACCTAGG GGATGGAGGC
    TGCTATGGAG ATCTATTTTG TAAAGCACTG AAAACATACA ATATGCTTTG CTTTGGAATT
    TATCGATTAA GGGACGCTCA TCTAAGTACT CCCAGCCAAT GCACTAAACG TTATGTTATC
    ACAAACCCAC CATATGAGTT TGAGCTTGTG CCGACAGACT TGATCTTCTG TTTGATGCAA
    TTCGACCACA ATGCTGGCCA GTCCCGGGCC AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT
    TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC TCCATCCCAT CAACAGCAAA CCGACCGAAC
    CGCACCAAGA CCAGGGATTC CCGGGAAAAA CAGAATGCAA CAAGGATGAA TAGAATGGGC
    CAAGAAAAGA AATGGTTTAC AGATGAACCG GATAATGCCT ATCCCAGAAA CATTCAAATC
    AAGCCCATGA GCACCCACAT GGCCAATCAG ATCAATCAAT ACAAATCGAC AAGCAGCTTG
    ATACCACCAA TCAGAGAAGT TGAAGATGAA TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143663.1
    CDS368..4003
    Translation

    Target ORF information:

    RefSeq Version XM_015288177.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X33, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288177.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCAAT GTTTGCTCGC
    TACGTGCCAG AAATTGCTGC TCTCATCCTT AATCGGAAGA AATACGGCGG GACTTTTAAC
    TCAACCCGAG GCAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGTAAAT GATTCAAATG TTCAGTTCTT GGACCAAGAT
    GATGATGATG ATCCAGATAC AGAACTGTAC CTCACGCAGC CCTTTGCATG TGGAACCGCA
    TTTGCTGTCA GCGTGCTGGA CTCTCTCATG AGCGCAACAT ACTTCAATGA CAATATCCTC
    ACACTGATAC GGACATTGGT AACAGGAGGA GCCACACCAG AGCTGGAAGC ACTGATTGCC
    GAGGAAAATG CTTTGAGAGG AGGTTACAGC ACGCCCCAGA CACTTGCAAA CAGGGACAGG
    TGTCGAGTTG CTCAGTTGGC TTTGTATGAT GGGCCATTTG CAGACCTAGG GGATGGAGGC
    TGCTATGGAG ATCTATTTTG TAAAGCACTG AAAACATACA ATATGCTTTG CTTTGGAATT
    TATCGATTAA GGGACGCTCA TCTAAGTACT CCCAGCCAAT GCACTAAACG TTATGTTATC
    ACAAACCCAC CATATGAGTT TGAGCTTGTG CCGACAGACT TGATCTTCTG TTTGATGCAA
    TTCGACCACA ATGCTGGCCA GTCCCGGGCC AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT
    TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC TCCATCCCAT CAACAGCAAA CCGACCGAAC
    CGCACCAAGA CCAGGGATTC CCGGGAAAAA CAGAATGCAA CAAGGATGAA TAGAATGGGC
    CAAGAAAAGA AATGGTTTAC AGATGAACCG GATAATGCCT ATCCCAGAAA CATTCAAATC
    AAGCCCATGA GCACCCACAT GGCCAATCAG ATCAATCAAT ACAAATCGAC AAGCAGCTTG
    ATACCACCAA TCAGAGAAGT TGAAGATGAA TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37450
    Clone ID Related Accession (Same CDS sequence) XM_015288177.2 , XM_015288177.1
    Accession Version XM_015288177.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X31
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCAAT GTTTGCTCGC
    TACGTGCCAG AAATTGCTGC TCTCATCCTT AATCGGAAGA AATACGGCGG GACTTTTAAC
    TCAACCCGAG GCAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGTAAAT GATTCAAATG TTCAGTTCTT GGACCAAGAT
    GATGATGATG ATCCAGATAC AGAACTGTAC CTCACGCAGC CCTTTGCATG TGGAACCGCA
    TTTGCTGTCA GCGTGCTGGA CTCTCTCATG AGCGCAACAT ACTTCAATGA CAATATCCTC
    ACACTGATAC GGACATTGGT AACAGGAGGA GCCACACCAG AGCTGGAAGC ACTGATTGCC
    GAGGAAAATG CTTTGAGAGG AGGTTACAGC ACGCCCCAGA CACTTGCAAA CAGGGACAGG
    TGTCGAGTTG CTCAGTTGGC TTTGTATGAT GGGCCATTTG CAGACCTAGG GGATGGAGGC
    TGCTATGGAG ATCTATTTTG TAAAGCACTG AAAACATACA ATATGCTTTG CTTTGGAATT
    TATCGATTAA GGGACGCTCA TCTAAGTACT CCCAGCCAAT GCACTAAACG TTATGTTATC
    ACAAACCCAC CATATGAGTT TGAGCTTGTG CCGACAGACT TGATCTTCTG TTTGATGCAA
    TTCGACCACA ATGCTGGCCA GTCCCGGGCC AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT
    TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC TCCATCCCAT CAACAGCAAA CCGACCGAAC
    CGCACCAAGA CCAGGGATTC CCGGGAAAAA CAGAATGCAA CAAGGATGAA TAGAATGGGC
    CAAGAAAAGA AATGGTTTAC AGATGAACCG GATAATGCCT ATCCCAGAAA CATTCAAATC
    AAGCCCATGA GCACCCACAT GGCCAATCAG ATCAATCAAT ACAAATCGAC AAGCAGCTTG
    ATACCACCAA TCAGAGAAGT TGAAGATGAA TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143663.1
    CDS373..4008
    Translation

    Target ORF information:

    RefSeq Version XM_015288177.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X31, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288177.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCAAT GTTTGCTCGC
    TACGTGCCAG AAATTGCTGC TCTCATCCTT AATCGGAAGA AATACGGCGG GACTTTTAAC
    TCAACCCGAG GCAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGTAAAT GATTCAAATG TTCAGTTCTT GGACCAAGAT
    GATGATGATG ATCCAGATAC AGAACTGTAC CTCACGCAGC CCTTTGCATG TGGAACCGCA
    TTTGCTGTCA GCGTGCTGGA CTCTCTCATG AGCGCAACAT ACTTCAATGA CAATATCCTC
    ACACTGATAC GGACATTGGT AACAGGAGGA GCCACACCAG AGCTGGAAGC ACTGATTGCC
    GAGGAAAATG CTTTGAGAGG AGGTTACAGC ACGCCCCAGA CACTTGCAAA CAGGGACAGG
    TGTCGAGTTG CTCAGTTGGC TTTGTATGAT GGGCCATTTG CAGACCTAGG GGATGGAGGC
    TGCTATGGAG ATCTATTTTG TAAAGCACTG AAAACATACA ATATGCTTTG CTTTGGAATT
    TATCGATTAA GGGACGCTCA TCTAAGTACT CCCAGCCAAT GCACTAAACG TTATGTTATC
    ACAAACCCAC CATATGAGTT TGAGCTTGTG CCGACAGACT TGATCTTCTG TTTGATGCAA
    TTCGACCACA ATGCTGGCCA GTCCCGGGCC AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT
    TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC TCCATCCCAT CAACAGCAAA CCGACCGAAC
    CGCACCAAGA CCAGGGATTC CCGGGAAAAA CAGAATGCAA CAAGGATGAA TAGAATGGGC
    CAAGAAAAGA AATGGTTTAC AGATGAACCG GATAATGCCT ATCCCAGAAA CATTCAAATC
    AAGCCCATGA GCACCCACAT GGCCAATCAG ATCAATCAAT ACAAATCGAC AAGCAGCTTG
    ATACCACCAA TCAGAGAAGT TGAAGATGAA TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37451
    Clone ID Related Accession (Same CDS sequence) XM_015288178.1 , XM_015288178.2
    Accession Version XM_015288178.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3630bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X32
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGTAAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT
    GATCCAGATA CAGAACTGTA CCTCACGCAG CCCTTTGCAT GTGGAACCGC ATTTGCTGTC
    AGCGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA
    CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC CGAGGAAAAT
    GCTTTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT
    GCTCAGTTGG CTTTGTATGA TGGGCCATTT GCAGACCTAG GGGATGGAGG CTGCTATGGA
    GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA
    AGGGACGCTC ATCTAAGTAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA
    CCATATGAGT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTCGACCAC
    AATGCTGGCC AGTCCCGGGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC
    AAGAAGAGCT CATCAGTTCA CTCCATCCCA TCAACAGCAA ACCGACCGAA CCGCACCAAG
    ACCAGGGATT CCCGGGAAAA ACAGAATGCA ACAAGGATGA ATAGAATGGG CCAAGCAGAA
    AAGAAATGGT TTACAGATGA ACCGGATAAT GCCTATCCCA GAAACATTCA AATCAAGCCC
    ATGAGCACCC ACATGGCCAA TCAGATCAAT CAATACAAAT CGACAAGCAG CTTGATACCA
    CCAATCAGAG AAGTTGAAGA TGAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143664.1
    CDS373..4002
    Translation

    Target ORF information:

    RefSeq Version XM_015288178.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X32, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288178.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGTAAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT
    GATCCAGATA CAGAACTGTA CCTCACGCAG CCCTTTGCAT GTGGAACCGC ATTTGCTGTC
    AGCGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA
    CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC CGAGGAAAAT
    GCTTTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT
    GCTCAGTTGG CTTTGTATGA TGGGCCATTT GCAGACCTAG GGGATGGAGG CTGCTATGGA
    GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA
    AGGGACGCTC ATCTAAGTAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA
    CCATATGAGT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTCGACCAC
    AATGCTGGCC AGTCCCGGGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC
    AAGAAGAGCT CATCAGTTCA CTCCATCCCA TCAACAGCAA ACCGACCGAA CCGCACCAAG
    ACCAGGGATT CCCGGGAAAA ACAGAATGCA ACAAGGATGA ATAGAATGGG CCAAGCAGAA
    AAGAAATGGT TTACAGATGA ACCGGATAAT GCCTATCCCA GAAACATTCA AATCAAGCCC
    ATGAGCACCC ACATGGCCAA TCAGATCAAT CAATACAAAT CGACAAGCAG CTTGATACCA
    CCAATCAGAG AAGTTGAAGA TGAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37451
    Clone ID Related Accession (Same CDS sequence) XM_015288178.1 , XM_015288178.2
    Accession Version XM_015288178.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3630bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X34
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288178.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGTAAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT
    GATCCAGATA CAGAACTGTA CCTCACGCAG CCCTTTGCAT GTGGAACCGC ATTTGCTGTC
    AGCGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA
    CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC CGAGGAAAAT
    GCTTTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT
    GCTCAGTTGG CTTTGTATGA TGGGCCATTT GCAGACCTAG GGGATGGAGG CTGCTATGGA
    GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA
    AGGGACGCTC ATCTAAGTAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA
    CCATATGAGT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTCGACCAC
    AATGCTGGCC AGTCCCGGGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC
    AAGAAGAGCT CATCAGTTCA CTCCATCCCA TCAACAGCAA ACCGACCGAA CCGCACCAAG
    ACCAGGGATT CCCGGGAAAA ACAGAATGCA ACAAGGATGA ATAGAATGGG CCAAGCAGAA
    AAGAAATGGT TTACAGATGA ACCGGATAAT GCCTATCCCA GAAACATTCA AATCAAGCCC
    ATGAGCACCC ACATGGCCAA TCAGATCAAT CAATACAAAT CGACAAGCAG CTTGATACCA
    CCAATCAGAG AAGTTGAAGA TGAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143664.1
    CDS367..3996
    Translation

    Target ORF information:

    RefSeq Version XM_015288178.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X34, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288178.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGTAAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT
    GATCCAGATA CAGAACTGTA CCTCACGCAG CCCTTTGCAT GTGGAACCGC ATTTGCTGTC
    AGCGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA
    CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC CGAGGAAAAT
    GCTTTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT
    GCTCAGTTGG CTTTGTATGA TGGGCCATTT GCAGACCTAG GGGATGGAGG CTGCTATGGA
    GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA
    AGGGACGCTC ATCTAAGTAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA
    CCATATGAGT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTCGACCAC
    AATGCTGGCC AGTCCCGGGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC
    AAGAAGAGCT CATCAGTTCA CTCCATCCCA TCAACAGCAA ACCGACCGAA CCGCACCAAG
    ACCAGGGATT CCCGGGAAAA ACAGAATGCA ACAAGGATGA ATAGAATGGG CCAAGCAGAA
    AAGAAATGGT TTACAGATGA ACCGGATAAT GCCTATCCCA GAAACATTCA AATCAAGCCC
    ATGAGCACCC ACATGGCCAA TCAGATCAAT CAATACAAAT CGACAAGCAG CTTGATACCA
    CCAATCAGAG AAGTTGAAGA TGAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37452
    Clone ID Related Accession (Same CDS sequence) XM_015288179.1
    Accession Version XM_015288179.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3627bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X35
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGTAAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT
    GATCCAGATA CAGAACTGTA CCTCACGCAG CCCTTTGCAT GTGGAACCGC ATTTGCTGTC
    AGCGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA
    CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC CGAGGAAAAT
    GCTTTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT
    GCTCAGTTGG CTTTGTATGA TGGGCCATTT GCAGACCTAG GGGATGGAGG CTGCTATGGA
    GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA
    AGGGACGCTC ATCTAAGTAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA
    CCATATGAGT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTCGACCAC
    AATGCTGGCC AGTCCCGGGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC
    AAGAAGAGCT CATCAGTTCA CTCCATCCCA TCAACAGCAA ACCGACCGAA CCGCACCAAG
    ACCAGGGATT CCCGGGAAAA ACAGAATGCA ACAAGGATGA ATAGAATGGG CCAAGAAAAG
    AAATGGTTTA CAGATGAACC GGATAATGCC TATCCCAGAA ACATTCAAAT CAAGCCCATG
    AGCACCCACA TGGCCAATCA GATCAATCAA TACAAATCGA CAAGCAGCTT GATACCACCA
    ATCAGAGAAG TTGAAGATGA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143665.1
    CDS116..3742
    Translation

    Target ORF information:

    RefSeq Version XM_015288179.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X35, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288179.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGTAAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT
    GATCCAGATA CAGAACTGTA CCTCACGCAG CCCTTTGCAT GTGGAACCGC ATTTGCTGTC
    AGCGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA
    CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC CGAGGAAAAT
    GCTTTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT
    GCTCAGTTGG CTTTGTATGA TGGGCCATTT GCAGACCTAG GGGATGGAGG CTGCTATGGA
    GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA
    AGGGACGCTC ATCTAAGTAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA
    CCATATGAGT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTCGACCAC
    AATGCTGGCC AGTCCCGGGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC
    AAGAAGAGCT CATCAGTTCA CTCCATCCCA TCAACAGCAA ACCGACCGAA CCGCACCAAG
    ACCAGGGATT CCCGGGAAAA ACAGAATGCA ACAAGGATGA ATAGAATGGG CCAAGAAAAG
    AAATGGTTTA CAGATGAACC GGATAATGCC TATCCCAGAA ACATTCAAAT CAAGCCCATG
    AGCACCCACA TGGCCAATCA GATCAATCAA TACAAATCGA CAAGCAGCTT GATACCACCA
    ATCAGAGAAG TTGAAGATGA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37453
    Clone ID Related Accession (Same CDS sequence) XM_015288180.2 , XM_015288180.1
    Accession Version XM_015288180.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3627bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X36
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288180.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCAAT GTTTGCTCGC
    TACGTGCCAG AAATTGCTGC TCTCATCCTT AATCGGAAGA AATACGGCGG GACTTTTAAC
    TCAACCCGAG GCAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGTAAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT
    GATCCAGATA CAGAACTGTA CCTCACGCAG CCCTTTGCAT GTGGAACCGC ATTTGCTGTC
    AGCGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA
    CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC CGAGGAAAAT
    GCTTTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT
    GCTCAGTTGG CTTTGTATGA TGGGCCATTT GCAGACCTAG GGGATGGAGG CTGCTATGGA
    GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA
    AGGGACGCTC ATCTAAGTAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA
    CCATATGAGT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTCGACCAC
    AATGCTGGCC AGTCCCGGGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC
    AAGAAGAGCT CATCAGTTCA CTCCATCCCA TCAACAGCAA ACCGACCGAA CCGCACCAAG
    ACCAGGGATT CCCGGGAAAA ACAGAATGCA ACAAGGATGA ATAGAATGGG CCAAGAAAAG
    AAATGGTTTA CAGATGAACC GGATAATGCC TATCCCAGAA ACATTCAAAT CAAGCCCATG
    AGCACCCACA TGGCCAATCA GATCAATCAA TACAAATCGA CAAGCAGCTT GATACCACCA
    ATCAGAGAAG TTGAAGATGA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143666.1
    CDS368..3994
    Translation

    Target ORF information:

    RefSeq Version XM_015288180.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X36, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288180.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCAAT GTTTGCTCGC
    TACGTGCCAG AAATTGCTGC TCTCATCCTT AATCGGAAGA AATACGGCGG GACTTTTAAC
    TCAACCCGAG GCAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGTAAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT
    GATCCAGATA CAGAACTGTA CCTCACGCAG CCCTTTGCAT GTGGAACCGC ATTTGCTGTC
    AGCGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA
    CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC CGAGGAAAAT
    GCTTTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT
    GCTCAGTTGG CTTTGTATGA TGGGCCATTT GCAGACCTAG GGGATGGAGG CTGCTATGGA
    GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA
    AGGGACGCTC ATCTAAGTAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA
    CCATATGAGT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTCGACCAC
    AATGCTGGCC AGTCCCGGGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC
    AAGAAGAGCT CATCAGTTCA CTCCATCCCA TCAACAGCAA ACCGACCGAA CCGCACCAAG
    ACCAGGGATT CCCGGGAAAA ACAGAATGCA ACAAGGATGA ATAGAATGGG CCAAGAAAAG
    AAATGGTTTA CAGATGAACC GGATAATGCC TATCCCAGAA ACATTCAAAT CAAGCCCATG
    AGCACCCACA TGGCCAATCA GATCAATCAA TACAAATCGA CAAGCAGCTT GATACCACCA
    ATCAGAGAAG TTGAAGATGA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37453
    Clone ID Related Accession (Same CDS sequence) XM_015288180.2 , XM_015288180.1
    Accession Version XM_015288180.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3627bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X34
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCAAT GTTTGCTCGC
    TACGTGCCAG AAATTGCTGC TCTCATCCTT AATCGGAAGA AATACGGCGG GACTTTTAAC
    TCAACCCGAG GCAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGTAAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT
    GATCCAGATA CAGAACTGTA CCTCACGCAG CCCTTTGCAT GTGGAACCGC ATTTGCTGTC
    AGCGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA
    CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC CGAGGAAAAT
    GCTTTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT
    GCTCAGTTGG CTTTGTATGA TGGGCCATTT GCAGACCTAG GGGATGGAGG CTGCTATGGA
    GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA
    AGGGACGCTC ATCTAAGTAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA
    CCATATGAGT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTCGACCAC
    AATGCTGGCC AGTCCCGGGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC
    AAGAAGAGCT CATCAGTTCA CTCCATCCCA TCAACAGCAA ACCGACCGAA CCGCACCAAG
    ACCAGGGATT CCCGGGAAAA ACAGAATGCA ACAAGGATGA ATAGAATGGG CCAAGAAAAG
    AAATGGTTTA CAGATGAACC GGATAATGCC TATCCCAGAA ACATTCAAAT CAAGCCCATG
    AGCACCCACA TGGCCAATCA GATCAATCAA TACAAATCGA CAAGCAGCTT GATACCACCA
    ATCAGAGAAG TTGAAGATGA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143666.1
    CDS373..3999
    Translation

    Target ORF information:

    RefSeq Version XM_015288180.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X34, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288180.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCAAT GTTTGCTCGC
    TACGTGCCAG AAATTGCTGC TCTCATCCTT AATCGGAAGA AATACGGCGG GACTTTTAAC
    TCAACCCGAG GCAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGTAAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT
    GATCCAGATA CAGAACTGTA CCTCACGCAG CCCTTTGCAT GTGGAACCGC ATTTGCTGTC
    AGCGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA
    CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC CGAGGAAAAT
    GCTTTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT
    GCTCAGTTGG CTTTGTATGA TGGGCCATTT GCAGACCTAG GGGATGGAGG CTGCTATGGA
    GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA
    AGGGACGCTC ATCTAAGTAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA
    CCATATGAGT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTCGACCAC
    AATGCTGGCC AGTCCCGGGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC
    AAGAAGAGCT CATCAGTTCA CTCCATCCCA TCAACAGCAA ACCGACCGAA CCGCACCAAG
    ACCAGGGATT CCCGGGAAAA ACAGAATGCA ACAAGGATGA ATAGAATGGG CCAAGAAAAG
    AAATGGTTTA CAGATGAACC GGATAATGCC TATCCCAGAA ACATTCAAAT CAAGCCCATG
    AGCACCCACA TGGCCAATCA GATCAATCAA TACAAATCGA CAAGCAGCTT GATACCACCA
    ATCAGAGAAG TTGAAGATGA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37454
    Clone ID Related Accession (Same CDS sequence) XM_015288181.2 , XM_015288181.1
    Accession Version XM_015288181.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X39
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288181.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTT
    GAAGATGAGC AGCCGTCAAC ACTATCACCC AAAAAAAAGC AACGAAATGG AGGCATGCGA
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAT
    GAACAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACGGG AATGTTTCAT
    TGGTGTCCAG CCAAGGATAT TGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACCCT GCATAACTTC
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGCTTTA CACCCCCTGG TATGGATAGG
    TCATCTCCAG ACAACAGTCC TGTCCACGGC CTGTTACGTC AACCCTCCAT TACAACAGGA
    GCCAACATCC CTATTATAAC AGAGCTAGCT AAGCCTGGCA AACTTCTGCC TTTGGTTTCA
    ATCAGTCAGG AAAAAAACAG TGGAACCCAC ATTCTAATGA TAACTGAACT GGTAAATGAT
    TCAAATGTTC AGTTCTTGGA CCAAGATGAT GATGATGATC CAGATACAGA ACTGTACCTC
    ACGCAGCCCT TTGCATGTGG AACCGCATTT GCTGTCAGCG TGCTGGACTC TCTCATGAGC
    GCAACATACT TCAATGACAA TATCCTCACA CTGATACGGA CATTGGTAAC AGGAGGAGCC
    ACACCAGAGC TGGAAGCACT GATTGCCGAG GAAAATGCTT TGAGAGGAGG TTACAGCACG
    CCCCAGACAC TTGCAAACAG GGACAGGTGT CGAGTTGCTC AGTTGGCTTT GTATGATGGG
    CCATTTGCAG ACCTAGGGGA TGGAGGCTGC TATGGAGATC TATTTTGTAA AGCACTGAAA
    ACATACAATA TGCTTTGCTT TGGAATTTAT CGATTAAGGG ACGCTCATCT AAGTACTCCC
    AGCCAATGCA CTAAACGTTA TGTTATCACA AACCCACCAT ATGAGTTTGA GCTTGTGCCG
    ACAGACTTGA TCTTCTGTTT GATGCAATTC GACCACAATG CTGGCCAGTC CCGGGCCAGT
    CTTTCTCATT CCTCCCATTC CTCCTATTCT TCCAGCAAGA AGAGCTCATC AGTTCACTCC
    ATCCCATCAA CAGCAAACCG ACCGAACCGC ACCAAGACCA GGGATTCCCG GGAAAAACAG
    AACAGAAAAG AAATGGTTTA CAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143667.1
    CDS368..3934
    Translation

    Target ORF information:

    RefSeq Version XM_015288181.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X39, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288181.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTT
    GAAGATGAGC AGCCGTCAAC ACTATCACCC AAAAAAAAGC AACGAAATGG AGGCATGCGA
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAT
    GAACAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACGGG AATGTTTCAT
    TGGTGTCCAG CCAAGGATAT TGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACCCT GCATAACTTC
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGCTTTA CACCCCCTGG TATGGATAGG
    TCATCTCCAG ACAACAGTCC TGTCCACGGC CTGTTACGTC AACCCTCCAT TACAACAGGA
    GCCAACATCC CTATTATAAC AGAGCTAGCT AAGCCTGGCA AACTTCTGCC TTTGGTTTCA
    ATCAGTCAGG AAAAAAACAG TGGAACCCAC ATTCTAATGA TAACTGAACT GGTAAATGAT
    TCAAATGTTC AGTTCTTGGA CCAAGATGAT GATGATGATC CAGATACAGA ACTGTACCTC
    ACGCAGCCCT TTGCATGTGG AACCGCATTT GCTGTCAGCG TGCTGGACTC TCTCATGAGC
    GCAACATACT TCAATGACAA TATCCTCACA CTGATACGGA CATTGGTAAC AGGAGGAGCC
    ACACCAGAGC TGGAAGCACT GATTGCCGAG GAAAATGCTT TGAGAGGAGG TTACAGCACG
    CCCCAGACAC TTGCAAACAG GGACAGGTGT CGAGTTGCTC AGTTGGCTTT GTATGATGGG
    CCATTTGCAG ACCTAGGGGA TGGAGGCTGC TATGGAGATC TATTTTGTAA AGCACTGAAA
    ACATACAATA TGCTTTGCTT TGGAATTTAT CGATTAAGGG ACGCTCATCT AAGTACTCCC
    AGCCAATGCA CTAAACGTTA TGTTATCACA AACCCACCAT ATGAGTTTGA GCTTGTGCCG
    ACAGACTTGA TCTTCTGTTT GATGCAATTC GACCACAATG CTGGCCAGTC CCGGGCCAGT
    CTTTCTCATT CCTCCCATTC CTCCTATTCT TCCAGCAAGA AGAGCTCATC AGTTCACTCC
    ATCCCATCAA CAGCAAACCG ACCGAACCGC ACCAAGACCA GGGATTCCCG GGAAAAACAG
    AACAGAAAAG AAATGGTTTA CAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37454
    Clone ID Related Accession (Same CDS sequence) XM_015288181.2 , XM_015288181.1
    Accession Version XM_015288181.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X35
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTT
    GAAGATGAGC AGCCGTCAAC ACTATCACCC AAAAAAAAGC AACGAAATGG AGGCATGCGA
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAT
    GAACAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACGGG AATGTTTCAT
    TGGTGTCCAG CCAAGGATAT TGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACCCT GCATAACTTC
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGCTTTA CACCCCCTGG TATGGATAGG
    TCATCTCCAG ACAACAGTCC TGTCCACGGC CTGTTACGTC AACCCTCCAT TACAACAGGA
    GCCAACATCC CTATTATAAC AGAGCTAGCT AAGCCTGGCA AACTTCTGCC TTTGGTTTCA
    ATCAGTCAGG AAAAAAACAG TGGAACCCAC ATTCTAATGA TAACTGAACT GGTAAATGAT
    TCAAATGTTC AGTTCTTGGA CCAAGATGAT GATGATGATC CAGATACAGA ACTGTACCTC
    ACGCAGCCCT TTGCATGTGG AACCGCATTT GCTGTCAGCG TGCTGGACTC TCTCATGAGC
    GCAACATACT TCAATGACAA TATCCTCACA CTGATACGGA CATTGGTAAC AGGAGGAGCC
    ACACCAGAGC TGGAAGCACT GATTGCCGAG GAAAATGCTT TGAGAGGAGG TTACAGCACG
    CCCCAGACAC TTGCAAACAG GGACAGGTGT CGAGTTGCTC AGTTGGCTTT GTATGATGGG
    CCATTTGCAG ACCTAGGGGA TGGAGGCTGC TATGGAGATC TATTTTGTAA AGCACTGAAA
    ACATACAATA TGCTTTGCTT TGGAATTTAT CGATTAAGGG ACGCTCATCT AAGTACTCCC
    AGCCAATGCA CTAAACGTTA TGTTATCACA AACCCACCAT ATGAGTTTGA GCTTGTGCCG
    ACAGACTTGA TCTTCTGTTT GATGCAATTC GACCACAATG CTGGCCAGTC CCGGGCCAGT
    CTTTCTCATT CCTCCCATTC CTCCTATTCT TCCAGCAAGA AGAGCTCATC AGTTCACTCC
    ATCCCATCAA CAGCAAACCG ACCGAACCGC ACCAAGACCA GGGATTCCCG GGAAAAACAG
    AACAGAAAAG AAATGGTTTA CAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143667.1
    CDS373..3939
    Translation

    Target ORF information:

    RefSeq Version XM_015288181.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X35, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288181.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTT
    GAAGATGAGC AGCCGTCAAC ACTATCACCC AAAAAAAAGC AACGAAATGG AGGCATGCGA
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAT
    GAACAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACGGG AATGTTTCAT
    TGGTGTCCAG CCAAGGATAT TGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACCCT GCATAACTTC
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGCTTTA CACCCCCTGG TATGGATAGG
    TCATCTCCAG ACAACAGTCC TGTCCACGGC CTGTTACGTC AACCCTCCAT TACAACAGGA
    GCCAACATCC CTATTATAAC AGAGCTAGCT AAGCCTGGCA AACTTCTGCC TTTGGTTTCA
    ATCAGTCAGG AAAAAAACAG TGGAACCCAC ATTCTAATGA TAACTGAACT GGTAAATGAT
    TCAAATGTTC AGTTCTTGGA CCAAGATGAT GATGATGATC CAGATACAGA ACTGTACCTC
    ACGCAGCCCT TTGCATGTGG AACCGCATTT GCTGTCAGCG TGCTGGACTC TCTCATGAGC
    GCAACATACT TCAATGACAA TATCCTCACA CTGATACGGA CATTGGTAAC AGGAGGAGCC
    ACACCAGAGC TGGAAGCACT GATTGCCGAG GAAAATGCTT TGAGAGGAGG TTACAGCACG
    CCCCAGACAC TTGCAAACAG GGACAGGTGT CGAGTTGCTC AGTTGGCTTT GTATGATGGG
    CCATTTGCAG ACCTAGGGGA TGGAGGCTGC TATGGAGATC TATTTTGTAA AGCACTGAAA
    ACATACAATA TGCTTTGCTT TGGAATTTAT CGATTAAGGG ACGCTCATCT AAGTACTCCC
    AGCCAATGCA CTAAACGTTA TGTTATCACA AACCCACCAT ATGAGTTTGA GCTTGTGCCG
    ACAGACTTGA TCTTCTGTTT GATGCAATTC GACCACAATG CTGGCCAGTC CCGGGCCAGT
    CTTTCTCATT CCTCCCATTC CTCCTATTCT TCCAGCAAGA AGAGCTCATC AGTTCACTCC
    ATCCCATCAA CAGCAAACCG ACCGAACCGC ACCAAGACCA GGGATTCCCG GGAAAAACAG
    AACAGAAAAG AAATGGTTTA CAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37455
    Clone ID Related Accession (Same CDS sequence) XM_015288182.1 , XM_015288182.2
    Accession Version XM_015288182.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3564bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X36
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGCTAAG CCTGGCAAAC TTCTGCCTTT GGTTTCAATC
    AGTCAGGAAA AAAACAGTGG AACCCACATT CTAATGATAA CTGAACTGGT AAATGATTCA
    AATGTTCAGT TCTTGGACCA AGATGATGAT GATGATCCAG ATACAGAACT GTACCTCACG
    CAGCCCTTTG CATGTGGAAC CGCATTTGCT GTCAGCGTGC TGGACTCTCT CATGAGCGCA
    ACATACTTCA ATGACAATAT CCTCACACTG ATACGGACAT TGGTAACAGG AGGAGCCACA
    CCAGAGCTGG AAGCACTGAT TGCCGAGGAA AATGCTTTGA GAGGAGGTTA CAGCACGCCC
    CAGACACTTG CAAACAGGGA CAGGTGTCGA GTTGCTCAGT TGGCTTTGTA TGATGGGCCA
    TTTGCAGACC TAGGGGATGG AGGCTGCTAT GGAGATCTAT TTTGTAAAGC ACTGAAAACA
    TACAATATGC TTTGCTTTGG AATTTATCGA TTAAGGGACG CTCATCTAAG TACTCCCAGC
    CAATGCACTA AACGTTATGT TATCACAAAC CCACCATATG AGTTTGAGCT TGTGCCGACA
    GACTTGATCT TCTGTTTGAT GCAATTCGAC CACAATGCTG GCCAGTCCCG GGCCAGTCTT
    TCTCATTCCT CCCATTCCTC CTATTCTTCC AGCAAGAAGA GCTCATCAGT TCACTCCATC
    CCATCAACAG CAAACCGACC GAACCGCACC AAGACCAGGG ATTCCCGGGA AAAACAGAAG
    TATGTGCAGG AAGATAGACT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143668.1
    CDS373..3936
    Translation

    Target ORF information:

    RefSeq Version XM_015288182.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X36, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288182.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGCTAAG CCTGGCAAAC TTCTGCCTTT GGTTTCAATC
    AGTCAGGAAA AAAACAGTGG AACCCACATT CTAATGATAA CTGAACTGGT AAATGATTCA
    AATGTTCAGT TCTTGGACCA AGATGATGAT GATGATCCAG ATACAGAACT GTACCTCACG
    CAGCCCTTTG CATGTGGAAC CGCATTTGCT GTCAGCGTGC TGGACTCTCT CATGAGCGCA
    ACATACTTCA ATGACAATAT CCTCACACTG ATACGGACAT TGGTAACAGG AGGAGCCACA
    CCAGAGCTGG AAGCACTGAT TGCCGAGGAA AATGCTTTGA GAGGAGGTTA CAGCACGCCC
    CAGACACTTG CAAACAGGGA CAGGTGTCGA GTTGCTCAGT TGGCTTTGTA TGATGGGCCA
    TTTGCAGACC TAGGGGATGG AGGCTGCTAT GGAGATCTAT TTTGTAAAGC ACTGAAAACA
    TACAATATGC TTTGCTTTGG AATTTATCGA TTAAGGGACG CTCATCTAAG TACTCCCAGC
    CAATGCACTA AACGTTATGT TATCACAAAC CCACCATATG AGTTTGAGCT TGTGCCGACA
    GACTTGATCT TCTGTTTGAT GCAATTCGAC CACAATGCTG GCCAGTCCCG GGCCAGTCTT
    TCTCATTCCT CCCATTCCTC CTATTCTTCC AGCAAGAAGA GCTCATCAGT TCACTCCATC
    CCATCAACAG CAAACCGACC GAACCGCACC AAGACCAGGG ATTCCCGGGA AAAACAGAAG
    TATGTGCAGG AAGATAGACT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37455
    Clone ID Related Accession (Same CDS sequence) XM_015288182.1 , XM_015288182.2
    Accession Version XM_015288182.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3564bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X40
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288182.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGCTAAG CCTGGCAAAC TTCTGCCTTT GGTTTCAATC
    AGTCAGGAAA AAAACAGTGG AACCCACATT CTAATGATAA CTGAACTGGT AAATGATTCA
    AATGTTCAGT TCTTGGACCA AGATGATGAT GATGATCCAG ATACAGAACT GTACCTCACG
    CAGCCCTTTG CATGTGGAAC CGCATTTGCT GTCAGCGTGC TGGACTCTCT CATGAGCGCA
    ACATACTTCA ATGACAATAT CCTCACACTG ATACGGACAT TGGTAACAGG AGGAGCCACA
    CCAGAGCTGG AAGCACTGAT TGCCGAGGAA AATGCTTTGA GAGGAGGTTA CAGCACGCCC
    CAGACACTTG CAAACAGGGA CAGGTGTCGA GTTGCTCAGT TGGCTTTGTA TGATGGGCCA
    TTTGCAGACC TAGGGGATGG AGGCTGCTAT GGAGATCTAT TTTGTAAAGC ACTGAAAACA
    TACAATATGC TTTGCTTTGG AATTTATCGA TTAAGGGACG CTCATCTAAG TACTCCCAGC
    CAATGCACTA AACGTTATGT TATCACAAAC CCACCATATG AGTTTGAGCT TGTGCCGACA
    GACTTGATCT TCTGTTTGAT GCAATTCGAC CACAATGCTG GCCAGTCCCG GGCCAGTCTT
    TCTCATTCCT CCCATTCCTC CTATTCTTCC AGCAAGAAGA GCTCATCAGT TCACTCCATC
    CCATCAACAG CAAACCGACC GAACCGCACC AAGACCAGGG ATTCCCGGGA AAAACAGAAG
    TATGTGCAGG AAGATAGACT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143668.1
    CDS367..3930
    Translation

    Target ORF information:

    RefSeq Version XM_015288182.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X40, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288182.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGCTAAG CCTGGCAAAC TTCTGCCTTT GGTTTCAATC
    AGTCAGGAAA AAAACAGTGG AACCCACATT CTAATGATAA CTGAACTGGT AAATGATTCA
    AATGTTCAGT TCTTGGACCA AGATGATGAT GATGATCCAG ATACAGAACT GTACCTCACG
    CAGCCCTTTG CATGTGGAAC CGCATTTGCT GTCAGCGTGC TGGACTCTCT CATGAGCGCA
    ACATACTTCA ATGACAATAT CCTCACACTG ATACGGACAT TGGTAACAGG AGGAGCCACA
    CCAGAGCTGG AAGCACTGAT TGCCGAGGAA AATGCTTTGA GAGGAGGTTA CAGCACGCCC
    CAGACACTTG CAAACAGGGA CAGGTGTCGA GTTGCTCAGT TGGCTTTGTA TGATGGGCCA
    TTTGCAGACC TAGGGGATGG AGGCTGCTAT GGAGATCTAT TTTGTAAAGC ACTGAAAACA
    TACAATATGC TTTGCTTTGG AATTTATCGA TTAAGGGACG CTCATCTAAG TACTCCCAGC
    CAATGCACTA AACGTTATGT TATCACAAAC CCACCATATG AGTTTGAGCT TGTGCCGACA
    GACTTGATCT TCTGTTTGAT GCAATTCGAC CACAATGCTG GCCAGTCCCG GGCCAGTCTT
    TCTCATTCCT CCCATTCCTC CTATTCTTCC AGCAAGAAGA GCTCATCAGT TCACTCCATC
    CCATCAACAG CAAACCGACC GAACCGCACC AAGACCAGGG ATTCCCGGGA AAAACAGAAG
    TATGTGCAGG AAGATAGACT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37456
    Clone ID Related Accession (Same CDS sequence) XM_015288183.1 , XM_015288183.2
    Accession Version XM_015288183.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X37
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGCTAAG CCTGGCAAAC TTCTGCCTTT GGTTTCAATC
    AGTCAGGAAA AAAACAGTGG AACCCACATT CTAATGATAA CTGAACTGGT AAATGATTCA
    AATGTTCAGT TCTTGGACCA AGATGATGAT GATGATCCAG ATACAGAACT GTACCTCACG
    CAGCCCTTTG CATGTGGAAC CGCATTTGCT GTCAGCGTGC TGGACTCTCT CATGAGCGCA
    ACATACTTCA ATGACAATAT CCTCACACTG ATACGGACAT TGGTAACAGG AGGAGCCACA
    CCAGAGCTGG AAGCACTGAT TGCCGAGGAA AATGCTTTGA GAGGAGGTTA CAGCACGCCC
    CAGACACTTG CAAACAGGGA CAGGTGTCGA GTTGCTCAGT TGGCTTTGTA TGATGGGCCA
    TTTGCAGACC TAGGGGATGG AGGCTGCTAT GGAGATCTAT TTTGTAAAGC ACTGAAAACA
    TACAATATGC TTTGCTTTGG AATTTATCGA TTAAGGGACG CTCATCTAAG TACTCCCAGC
    CAATGCACTA AACGTTATGT TATCACAAAC CCACCATATG AGTTTGAGCT TGTGCCGACA
    GACTTGATCT TCTGTTTGAT GCAATTCGAC CACAATGCTG GCCAGTCCCG GGCCAGTCTT
    TCTCATTCCT CCCATTCCTC CTATTCTTCC AGCAAGAAGA GCTCATCAGT TCACTCCATC
    CCATCAACAG CAAACCGACC GAACCGCACC AAGACCAGGG ATTCCCGGGA AAAACAGAAA
    AAAGAAATGG TTTACAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143669.1
    CDS373..3933
    Translation

    Target ORF information:

    RefSeq Version XM_015288183.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X37, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288183.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGCTAAG CCTGGCAAAC TTCTGCCTTT GGTTTCAATC
    AGTCAGGAAA AAAACAGTGG AACCCACATT CTAATGATAA CTGAACTGGT AAATGATTCA
    AATGTTCAGT TCTTGGACCA AGATGATGAT GATGATCCAG ATACAGAACT GTACCTCACG
    CAGCCCTTTG CATGTGGAAC CGCATTTGCT GTCAGCGTGC TGGACTCTCT CATGAGCGCA
    ACATACTTCA ATGACAATAT CCTCACACTG ATACGGACAT TGGTAACAGG AGGAGCCACA
    CCAGAGCTGG AAGCACTGAT TGCCGAGGAA AATGCTTTGA GAGGAGGTTA CAGCACGCCC
    CAGACACTTG CAAACAGGGA CAGGTGTCGA GTTGCTCAGT TGGCTTTGTA TGATGGGCCA
    TTTGCAGACC TAGGGGATGG AGGCTGCTAT GGAGATCTAT TTTGTAAAGC ACTGAAAACA
    TACAATATGC TTTGCTTTGG AATTTATCGA TTAAGGGACG CTCATCTAAG TACTCCCAGC
    CAATGCACTA AACGTTATGT TATCACAAAC CCACCATATG AGTTTGAGCT TGTGCCGACA
    GACTTGATCT TCTGTTTGAT GCAATTCGAC CACAATGCTG GCCAGTCCCG GGCCAGTCTT
    TCTCATTCCT CCCATTCCTC CTATTCTTCC AGCAAGAAGA GCTCATCAGT TCACTCCATC
    CCATCAACAG CAAACCGACC GAACCGCACC AAGACCAGGG ATTCCCGGGA AAAACAGAAA
    AAAGAAATGG TTTACAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37456
    Clone ID Related Accession (Same CDS sequence) XM_015288183.1 , XM_015288183.2
    Accession Version XM_015288183.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X42
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288183.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGCTAAG CCTGGCAAAC TTCTGCCTTT GGTTTCAATC
    AGTCAGGAAA AAAACAGTGG AACCCACATT CTAATGATAA CTGAACTGGT AAATGATTCA
    AATGTTCAGT TCTTGGACCA AGATGATGAT GATGATCCAG ATACAGAACT GTACCTCACG
    CAGCCCTTTG CATGTGGAAC CGCATTTGCT GTCAGCGTGC TGGACTCTCT CATGAGCGCA
    ACATACTTCA ATGACAATAT CCTCACACTG ATACGGACAT TGGTAACAGG AGGAGCCACA
    CCAGAGCTGG AAGCACTGAT TGCCGAGGAA AATGCTTTGA GAGGAGGTTA CAGCACGCCC
    CAGACACTTG CAAACAGGGA CAGGTGTCGA GTTGCTCAGT TGGCTTTGTA TGATGGGCCA
    TTTGCAGACC TAGGGGATGG AGGCTGCTAT GGAGATCTAT TTTGTAAAGC ACTGAAAACA
    TACAATATGC TTTGCTTTGG AATTTATCGA TTAAGGGACG CTCATCTAAG TACTCCCAGC
    CAATGCACTA AACGTTATGT TATCACAAAC CCACCATATG AGTTTGAGCT TGTGCCGACA
    GACTTGATCT TCTGTTTGAT GCAATTCGAC CACAATGCTG GCCAGTCCCG GGCCAGTCTT
    TCTCATTCCT CCCATTCCTC CTATTCTTCC AGCAAGAAGA GCTCATCAGT TCACTCCATC
    CCATCAACAG CAAACCGACC GAACCGCACC AAGACCAGGG ATTCCCGGGA AAAACAGAAA
    AAAGAAATGG TTTACAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143669.1
    CDS368..3928
    Translation

    Target ORF information:

    RefSeq Version XM_015288183.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X42, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288183.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTGAT CTATTTTGAA
    GATGAGCAGC CGTCAACACT ATCACCCAAA AAAAAGCAAC GAAATGGAGG CATGCGAAAT
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAATGAA
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACGGGAAT GTTTCATTGG
    TGTCCAGCCA AGGATATTGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACCCTGCA TAACTTCCCC
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGCTTTACAC CCCCTGGTAT GGATAGGTCA
    TCTCCAGACA ACAGTCCTGT CCACGGCCTG TTACGTCAAC CCTCCATTAC AACAGGAGCC
    AACATCCCTA TTATAACAGA GCTAGCTAAG CCTGGCAAAC TTCTGCCTTT GGTTTCAATC
    AGTCAGGAAA AAAACAGTGG AACCCACATT CTAATGATAA CTGAACTGGT AAATGATTCA
    AATGTTCAGT TCTTGGACCA AGATGATGAT GATGATCCAG ATACAGAACT GTACCTCACG
    CAGCCCTTTG CATGTGGAAC CGCATTTGCT GTCAGCGTGC TGGACTCTCT CATGAGCGCA
    ACATACTTCA ATGACAATAT CCTCACACTG ATACGGACAT TGGTAACAGG AGGAGCCACA
    CCAGAGCTGG AAGCACTGAT TGCCGAGGAA AATGCTTTGA GAGGAGGTTA CAGCACGCCC
    CAGACACTTG CAAACAGGGA CAGGTGTCGA GTTGCTCAGT TGGCTTTGTA TGATGGGCCA
    TTTGCAGACC TAGGGGATGG AGGCTGCTAT GGAGATCTAT TTTGTAAAGC ACTGAAAACA
    TACAATATGC TTTGCTTTGG AATTTATCGA TTAAGGGACG CTCATCTAAG TACTCCCAGC
    CAATGCACTA AACGTTATGT TATCACAAAC CCACCATATG AGTTTGAGCT TGTGCCGACA
    GACTTGATCT TCTGTTTGAT GCAATTCGAC CACAATGCTG GCCAGTCCCG GGCCAGTCTT
    TCTCATTCCT CCCATTCCTC CTATTCTTCC AGCAAGAAGA GCTCATCAGT TCACTCCATC
    CCATCAACAG CAAACCGACC GAACCGCACC AAGACCAGGG ATTCCCGGGA AAAACAGAAA
    AAAGAAATGG TTTACAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37457
    Clone ID Related Accession (Same CDS sequence) XM_015288184.2 , XM_015288184.1
    Accession Version XM_015288184.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X43
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288184.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTT
    GAAGATGAGC AGCCGTCAAC ACTATCACCC AAAAAAAAGC AACGAAATGG AGGCATGCGA
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAT
    GAACAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACGGG AATGTTTCAT
    TGGTGTCCAG CCAAGGATAT TGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACCCT GCATAACTTC
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGCTTTA CACCCCCTGG TATGGATAGG
    TCATCTCCAG ACAACAGTCC TGTCCACGGC CTGTTACGTC AACCCTCCAT TACAACAGGA
    GCCAACATCC CTATTATAAC AGAGCTAGTA AATGATTCAA ATGTTCAGTT CTTGGACCAA
    GATGATGATG ATGATCCAGA TACAGAACTG TACCTCACGC AGCCCTTTGC ATGTGGAACC
    GCATTTGCTG TCAGCGTGCT GGACTCTCTC ATGAGCGCAA CATACTTCAA TGACAATATC
    CTCACACTGA TACGGACATT GGTAACAGGA GGAGCCACAC CAGAGCTGGA AGCACTGATT
    GCCGAGGAAA ATGCTTTGAG AGGAGGTTAC AGCACGCCCC AGACACTTGC AAACAGGGAC
    AGGTGTCGAG TTGCTCAGTT GGCTTTGTAT GATGGGCCAT TTGCAGACCT AGGGGATGGA
    GGCTGCTATG GAGATCTATT TTGTAAAGCA CTGAAAACAT ACAATATGCT TTGCTTTGGA
    ATTTATCGAT TAAGGGACGC TCATCTAAGT ACTCCCAGCC AATGCACTAA ACGTTATGTT
    ATCACAAACC CACCATATGA GTTTGAGCTT GTGCCGACAG ACTTGATCTT CTGTTTGATG
    CAATTCGACC ACAATGCTGG CCAGTCCCGG GCCAGTCTTT CTCATTCCTC CCATTCCTCC
    TATTCTTCCA GCAAGAAGAG CTCATCAGTT CACTCCATCC CATCAACAGC AAACCGACCG
    AACCGCACCA AGACCAGGGA TTCCCGGGAA AAACAGAACA GAAAAGAAAT GGTTTACAGA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143670.1
    CDS367..3849
    Translation

    Target ORF information:

    RefSeq Version XM_015288184.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X43, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288184.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTT
    GAAGATGAGC AGCCGTCAAC ACTATCACCC AAAAAAAAGC AACGAAATGG AGGCATGCGA
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAT
    GAACAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACGGG AATGTTTCAT
    TGGTGTCCAG CCAAGGATAT TGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACCCT GCATAACTTC
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGCTTTA CACCCCCTGG TATGGATAGG
    TCATCTCCAG ACAACAGTCC TGTCCACGGC CTGTTACGTC AACCCTCCAT TACAACAGGA
    GCCAACATCC CTATTATAAC AGAGCTAGTA AATGATTCAA ATGTTCAGTT CTTGGACCAA
    GATGATGATG ATGATCCAGA TACAGAACTG TACCTCACGC AGCCCTTTGC ATGTGGAACC
    GCATTTGCTG TCAGCGTGCT GGACTCTCTC ATGAGCGCAA CATACTTCAA TGACAATATC
    CTCACACTGA TACGGACATT GGTAACAGGA GGAGCCACAC CAGAGCTGGA AGCACTGATT
    GCCGAGGAAA ATGCTTTGAG AGGAGGTTAC AGCACGCCCC AGACACTTGC AAACAGGGAC
    AGGTGTCGAG TTGCTCAGTT GGCTTTGTAT GATGGGCCAT TTGCAGACCT AGGGGATGGA
    GGCTGCTATG GAGATCTATT TTGTAAAGCA CTGAAAACAT ACAATATGCT TTGCTTTGGA
    ATTTATCGAT TAAGGGACGC TCATCTAAGT ACTCCCAGCC AATGCACTAA ACGTTATGTT
    ATCACAAACC CACCATATGA GTTTGAGCTT GTGCCGACAG ACTTGATCTT CTGTTTGATG
    CAATTCGACC ACAATGCTGG CCAGTCCCGG GCCAGTCTTT CTCATTCCTC CCATTCCTCC
    TATTCTTCCA GCAAGAAGAG CTCATCAGTT CACTCCATCC CATCAACAGC AAACCGACCG
    AACCGCACCA AGACCAGGGA TTCCCGGGAA AAACAGAACA GAAAAGAAAT GGTTTACAGA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37457
    Clone ID Related Accession (Same CDS sequence) XM_015288184.2 , XM_015288184.1
    Accession Version XM_015288184.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X38
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTT
    GAAGATGAGC AGCCGTCAAC ACTATCACCC AAAAAAAAGC AACGAAATGG AGGCATGCGA
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAT
    GAACAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACGGG AATGTTTCAT
    TGGTGTCCAG CCAAGGATAT TGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACCCT GCATAACTTC
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGCTTTA CACCCCCTGG TATGGATAGG
    TCATCTCCAG ACAACAGTCC TGTCCACGGC CTGTTACGTC AACCCTCCAT TACAACAGGA
    GCCAACATCC CTATTATAAC AGAGCTAGTA AATGATTCAA ATGTTCAGTT CTTGGACCAA
    GATGATGATG ATGATCCAGA TACAGAACTG TACCTCACGC AGCCCTTTGC ATGTGGAACC
    GCATTTGCTG TCAGCGTGCT GGACTCTCTC ATGAGCGCAA CATACTTCAA TGACAATATC
    CTCACACTGA TACGGACATT GGTAACAGGA GGAGCCACAC CAGAGCTGGA AGCACTGATT
    GCCGAGGAAA ATGCTTTGAG AGGAGGTTAC AGCACGCCCC AGACACTTGC AAACAGGGAC
    AGGTGTCGAG TTGCTCAGTT GGCTTTGTAT GATGGGCCAT TTGCAGACCT AGGGGATGGA
    GGCTGCTATG GAGATCTATT TTGTAAAGCA CTGAAAACAT ACAATATGCT TTGCTTTGGA
    ATTTATCGAT TAAGGGACGC TCATCTAAGT ACTCCCAGCC AATGCACTAA ACGTTATGTT
    ATCACAAACC CACCATATGA GTTTGAGCTT GTGCCGACAG ACTTGATCTT CTGTTTGATG
    CAATTCGACC ACAATGCTGG CCAGTCCCGG GCCAGTCTTT CTCATTCCTC CCATTCCTCC
    TATTCTTCCA GCAAGAAGAG CTCATCAGTT CACTCCATCC CATCAACAGC AAACCGACCG
    AACCGCACCA AGACCAGGGA TTCCCGGGAA AAACAGAACA GAAAAGAAAT GGTTTACAGA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143670.1
    CDS372..3854
    Translation

    Target ORF information:

    RefSeq Version XM_015288184.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X38, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288184.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTT
    GAAGATGAGC AGCCGTCAAC ACTATCACCC AAAAAAAAGC AACGAAATGG AGGCATGCGA
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAT
    GAACAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACGGG AATGTTTCAT
    TGGTGTCCAG CCAAGGATAT TGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACCCT GCATAACTTC
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGCTTTA CACCCCCTGG TATGGATAGG
    TCATCTCCAG ACAACAGTCC TGTCCACGGC CTGTTACGTC AACCCTCCAT TACAACAGGA
    GCCAACATCC CTATTATAAC AGAGCTAGTA AATGATTCAA ATGTTCAGTT CTTGGACCAA
    GATGATGATG ATGATCCAGA TACAGAACTG TACCTCACGC AGCCCTTTGC ATGTGGAACC
    GCATTTGCTG TCAGCGTGCT GGACTCTCTC ATGAGCGCAA CATACTTCAA TGACAATATC
    CTCACACTGA TACGGACATT GGTAACAGGA GGAGCCACAC CAGAGCTGGA AGCACTGATT
    GCCGAGGAAA ATGCTTTGAG AGGAGGTTAC AGCACGCCCC AGACACTTGC AAACAGGGAC
    AGGTGTCGAG TTGCTCAGTT GGCTTTGTAT GATGGGCCAT TTGCAGACCT AGGGGATGGA
    GGCTGCTATG GAGATCTATT TTGTAAAGCA CTGAAAACAT ACAATATGCT TTGCTTTGGA
    ATTTATCGAT TAAGGGACGC TCATCTAAGT ACTCCCAGCC AATGCACTAA ACGTTATGTT
    ATCACAAACC CACCATATGA GTTTGAGCTT GTGCCGACAG ACTTGATCTT CTGTTTGATG
    CAATTCGACC ACAATGCTGG CCAGTCCCGG GCCAGTCTTT CTCATTCCTC CCATTCCTCC
    TATTCTTCCA GCAAGAAGAG CTCATCAGTT CACTCCATCC CATCAACAGC AAACCGACCG
    AACCGCACCA AGACCAGGGA TTCCCGGGAA AAACAGAACA GAAAAGAAAT GGTTTACAGA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37458
    Clone ID Related Accession (Same CDS sequence) XM_015288185.1
    Accession Version XM_015288185.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3480bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X45
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTT
    GAAGATGAGC AGCCGTCAAC ACTATCACCC AAAAAAAAGC AACGAAATGG AGGCATGCGA
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAT
    GAACAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACGGG AATGTTTCAT
    TGGTGTCCAG CCAAGGATAT TGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACCCT GCATAACTTC
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGCTTTA CACCCCCTGG TATGGATAGG
    TCATCTCCAG ACAACAGTCC TGTCCACGGC CTGTTACGTC AACCCTCCAT TACAACAGGA
    GCCAACATCC CTATTATAAC AGAGCTAGTA AATGATTCAA ATGTTCAGTT CTTGGACCAA
    GATGATGATG ATGATCCAGA TACAGAACTG TACCTCACGC AGCCCTTTGC ATGTGGAACC
    GCATTTGCTG TCAGCGTGCT GGACTCTCTC ATGAGCGCAA CATACTTCAA TGACAATATC
    CTCACACTGA TACGGACATT GGTAACAGGA GGAGCCACAC CAGAGCTGGA AGCACTGATT
    GCCGAGGAAA ATGCTTTGAG AGGAGGTTAC AGCACGCCCC AGACACTTGC AAACAGGGAC
    AGGTGTCGAG TTGCTCAGTT GGCTTTGTAT GATGGGCCAT TTGCAGACCT AGGGGATGGA
    GGCTGCTATG GAGATCTATT TTGTAAAGCA CTGAAAACAT ACAATATGCT TTGCTTTGGA
    ATTTATCGAT TAAGGGACGC TCATCTAAGT ACTCCCAGCC AATGCACTAA ACGTTATGTT
    ATCACAAACC CACCATATGA GTTTGAGCTT GTGCCGACAG ACTTGATCTT CTGTTTGATG
    CAATTCGACC ACAATGCTGG CCAGTCCCGG GCCAGTCTTT CTCATTCCTC CCATTCCTCC
    TATTCTTCCA GCAAGAAGAG CTCATCAGTT CACTCCATCC CATCAACAGC AAACCGACCG
    AACCGCACCA AGACCAGGGA TTCCCGGGAA AAACAGAAAA AAGAAATGGT TTACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143671.1
    CDS116..3595
    Translation

    Target ORF information:

    RefSeq Version XM_015288185.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X45, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288185.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGAAGCCGAA AGCGTATATT GATCAATCCT GGAAACCATG TGAAGATTCA AGAAGGTACC
    TTAGGATTCT TCATTGCAAG TGATGCCAAA GAAGTAAAAA GGGCATTTTT TTACTGCAAG
    GCCTGCCATG ATGACATCAC AGATCCCAAA AGGATAAAAA AGTGTGGCTG CAAACGGCTT
    GAAGATGAGC AGCCGTCAAC ACTATCACCC AAAAAAAAGC AACGAAATGG AGGCATGCGA
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAT
    GAACAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACGGG AATGTTTCAT
    TGGTGTCCAG CCAAGGATAT TGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACCCT GCATAACTTC
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGCTTTA CACCCCCTGG TATGGATAGG
    TCATCTCCAG ACAACAGTCC TGTCCACGGC CTGTTACGTC AACCCTCCAT TACAACAGGA
    GCCAACATCC CTATTATAAC AGAGCTAGTA AATGATTCAA ATGTTCAGTT CTTGGACCAA
    GATGATGATG ATGATCCAGA TACAGAACTG TACCTCACGC AGCCCTTTGC ATGTGGAACC
    GCATTTGCTG TCAGCGTGCT GGACTCTCTC ATGAGCGCAA CATACTTCAA TGACAATATC
    CTCACACTGA TACGGACATT GGTAACAGGA GGAGCCACAC CAGAGCTGGA AGCACTGATT
    GCCGAGGAAA ATGCTTTGAG AGGAGGTTAC AGCACGCCCC AGACACTTGC AAACAGGGAC
    AGGTGTCGAG TTGCTCAGTT GGCTTTGTAT GATGGGCCAT TTGCAGACCT AGGGGATGGA
    GGCTGCTATG GAGATCTATT TTGTAAAGCA CTGAAAACAT ACAATATGCT TTGCTTTGGA
    ATTTATCGAT TAAGGGACGC TCATCTAAGT ACTCCCAGCC AATGCACTAA ACGTTATGTT
    ATCACAAACC CACCATATGA GTTTGAGCTT GTGCCGACAG ACTTGATCTT CTGTTTGATG
    CAATTCGACC ACAATGCTGG CCAGTCCCGG GCCAGTCTTT CTCATTCCTC CCATTCCTCC
    TATTCTTCCA GCAAGAAGAG CTCATCAGTT CACTCCATCC CATCAACAGC AAACCGACCG
    AACCGCACCA AGACCAGGGA TTCCCGGGAA AAACAGAAAA AAGAAATGGT TTACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37459
    Clone ID Related Accession (Same CDS sequence) XM_015288186.2 , XM_015288186.1
    Accession Version XM_015288186.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X47
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288186.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGTAAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT
    GATCCAGATA CAGAACTGTA CCTCACGCAG CCCTTTGCAT GTGGAACCGC ATTTGCTGTC
    AGCGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA
    CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC CGAGGAAAAT
    GCTTTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT
    GCTCAGTTGG CTTTGTATGA TGGGCCATTT GCAGACCTAG GGGATGGAGG CTGCTATGGA
    GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA
    AGGGACGCTC ATCTAAGTAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA
    CCATATGAGT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTCGACCAC
    AATGCTGGCC AGTCCCGGGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC
    AAGAAGAGCT CATCAGTTCA CTCCATCCCA TCAACAGCAA ACCGACCGAA CCGCACCAAG
    ACCAGGGATT CCCGGGAAAA ACAGAAGTAT GTGCAGGAAG ATAGACTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143672.1
    CDS116..3586
    Translation

    Target ORF information:

    RefSeq Version XM_015288186.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X47, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288186.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGTAAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT
    GATCCAGATA CAGAACTGTA CCTCACGCAG CCCTTTGCAT GTGGAACCGC ATTTGCTGTC
    AGCGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA
    CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC CGAGGAAAAT
    GCTTTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT
    GCTCAGTTGG CTTTGTATGA TGGGCCATTT GCAGACCTAG GGGATGGAGG CTGCTATGGA
    GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA
    AGGGACGCTC ATCTAAGTAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA
    CCATATGAGT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTCGACCAC
    AATGCTGGCC AGTCCCGGGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC
    AAGAAGAGCT CATCAGTTCA CTCCATCCCA TCAACAGCAA ACCGACCGAA CCGCACCAAG
    ACCAGGGATT CCCGGGAAAA ACAGAAGTAT GTGCAGGAAG ATAGACTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37459
    Clone ID Related Accession (Same CDS sequence) XM_015288186.2 , XM_015288186.1
    Accession Version XM_015288186.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X40
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGTAAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT
    GATCCAGATA CAGAACTGTA CCTCACGCAG CCCTTTGCAT GTGGAACCGC ATTTGCTGTC
    AGCGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA
    CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC CGAGGAAAAT
    GCTTTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT
    GCTCAGTTGG CTTTGTATGA TGGGCCATTT GCAGACCTAG GGGATGGAGG CTGCTATGGA
    GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA
    AGGGACGCTC ATCTAAGTAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA
    CCATATGAGT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTCGACCAC
    AATGCTGGCC AGTCCCGGGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC
    AAGAAGAGCT CATCAGTTCA CTCCATCCCA TCAACAGCAA ACCGACCGAA CCGCACCAAG
    ACCAGGGATT CCCGGGAAAA ACAGAAGTAT GTGCAGGAAG ATAGACTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143672.1
    CDS116..3586
    Translation

    Target ORF information:

    RefSeq Version XM_015288186.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X40, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288186.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGTAAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT
    GATCCAGATA CAGAACTGTA CCTCACGCAG CCCTTTGCAT GTGGAACCGC ATTTGCTGTC
    AGCGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA
    CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC CGAGGAAAAT
    GCTTTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT
    GCTCAGTTGG CTTTGTATGA TGGGCCATTT GCAGACCTAG GGGATGGAGG CTGCTATGGA
    GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA
    AGGGACGCTC ATCTAAGTAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA
    CCATATGAGT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTCGACCAC
    AATGCTGGCC AGTCCCGGGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC
    AAGAAGAGCT CATCAGTTCA CTCCATCCCA TCAACAGCAA ACCGACCGAA CCGCACCAAG
    ACCAGGGATT CCCGGGAAAA ACAGAAGTAT GTGCAGGAAG ATAGACTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37460
    Clone ID Related Accession (Same CDS sequence) XM_015288187.1 , XM_015288187.2
    Accession Version XM_015288187.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X41
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGTAAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT
    GATCCAGATA CAGAACTGTA CCTCACGCAG CCCTTTGCAT GTGGAACCGC ATTTGCTGTC
    AGCGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA
    CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC CGAGGAAAAT
    GCTTTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT
    GCTCAGTTGG CTTTGTATGA TGGGCCATTT GCAGACCTAG GGGATGGAGG CTGCTATGGA
    GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA
    AGGGACGCTC ATCTAAGTAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA
    CCATATGAGT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTCGACCAC
    AATGCTGGCC AGTCCCGGGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC
    AAGAAGAGCT CATCAGTTCA CTCCATCCCA TCAACAGCAA ACCGACCGAA CCGCACCAAG
    ACCAGGGATT CCCGGGAAAA ACAGAACAGA AAAGAAATGG TTTACAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143673.1
    CDS372..3842
    Translation

    Target ORF information:

    RefSeq Version XM_015288187.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X41, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288187.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGTAAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT
    GATCCAGATA CAGAACTGTA CCTCACGCAG CCCTTTGCAT GTGGAACCGC ATTTGCTGTC
    AGCGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA
    CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC CGAGGAAAAT
    GCTTTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT
    GCTCAGTTGG CTTTGTATGA TGGGCCATTT GCAGACCTAG GGGATGGAGG CTGCTATGGA
    GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA
    AGGGACGCTC ATCTAAGTAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA
    CCATATGAGT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTCGACCAC
    AATGCTGGCC AGTCCCGGGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC
    AAGAAGAGCT CATCAGTTCA CTCCATCCCA TCAACAGCAA ACCGACCGAA CCGCACCAAG
    ACCAGGGATT CCCGGGAAAA ACAGAACAGA AAAGAAATGG TTTACAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37460
    Clone ID Related Accession (Same CDS sequence) XM_015288187.1 , XM_015288187.2
    Accession Version XM_015288187.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X48
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288187.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGTAAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT
    GATCCAGATA CAGAACTGTA CCTCACGCAG CCCTTTGCAT GTGGAACCGC ATTTGCTGTC
    AGCGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA
    CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC CGAGGAAAAT
    GCTTTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT
    GCTCAGTTGG CTTTGTATGA TGGGCCATTT GCAGACCTAG GGGATGGAGG CTGCTATGGA
    GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA
    AGGGACGCTC ATCTAAGTAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA
    CCATATGAGT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTCGACCAC
    AATGCTGGCC AGTCCCGGGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC
    AAGAAGAGCT CATCAGTTCA CTCCATCCCA TCAACAGCAA ACCGACCGAA CCGCACCAAG
    ACCAGGGATT CCCGGGAAAA ACAGAACAGA AAAGAAATGG TTTACAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143673.1
    CDS367..3837
    Translation

    Target ORF information:

    RefSeq Version XM_015288187.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X48, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288187.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGTAAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT
    GATCCAGATA CAGAACTGTA CCTCACGCAG CCCTTTGCAT GTGGAACCGC ATTTGCTGTC
    AGCGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA
    CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC CGAGGAAAAT
    GCTTTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT
    GCTCAGTTGG CTTTGTATGA TGGGCCATTT GCAGACCTAG GGGATGGAGG CTGCTATGGA
    GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA
    AGGGACGCTC ATCTAAGTAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA
    CCATATGAGT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTCGACCAC
    AATGCTGGCC AGTCCCGGGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC
    AAGAAGAGCT CATCAGTTCA CTCCATCCCA TCAACAGCAA ACCGACCGAA CCGCACCAAG
    ACCAGGGATT CCCGGGAAAA ACAGAACAGA AAAGAAATGG TTTACAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37461
    Clone ID Related Accession (Same CDS sequence) XM_015288189.1 , XM_015288189.2
    Accession Version XM_015288189.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3468bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X42
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGTAAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT
    GATCCAGATA CAGAACTGTA CCTCACGCAG CCCTTTGCAT GTGGAACCGC ATTTGCTGTC
    AGCGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA
    CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC CGAGGAAAAT
    GCTTTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT
    GCTCAGTTGG CTTTGTATGA TGGGCCATTT GCAGACCTAG GGGATGGAGG CTGCTATGGA
    GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA
    AGGGACGCTC ATCTAAGTAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA
    CCATATGAGT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTCGACCAC
    AATGCTGGCC AGTCCCGGGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC
    AAGAAGAGCT CATCAGTTCA CTCCATCCCA TCAACAGCAA ACCGACCGAA CCGCACCAAG
    ACCAGGGATT CCCGGGAAAA ACAGAAAAAA GAAATGGTTT ACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143675.1
    CDS372..3839
    Translation

    Target ORF information:

    RefSeq Version XM_015288189.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X42, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288189.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGTAAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT
    GATCCAGATA CAGAACTGTA CCTCACGCAG CCCTTTGCAT GTGGAACCGC ATTTGCTGTC
    AGCGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA
    CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC CGAGGAAAAT
    GCTTTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT
    GCTCAGTTGG CTTTGTATGA TGGGCCATTT GCAGACCTAG GGGATGGAGG CTGCTATGGA
    GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA
    AGGGACGCTC ATCTAAGTAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA
    CCATATGAGT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTCGACCAC
    AATGCTGGCC AGTCCCGGGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC
    AAGAAGAGCT CATCAGTTCA CTCCATCCCA TCAACAGCAA ACCGACCGAA CCGCACCAAG
    ACCAGGGATT CCCGGGAAAA ACAGAAAAAA GAAATGGTTT ACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37461
    Clone ID Related Accession (Same CDS sequence) XM_015288189.1 , XM_015288189.2
    Accession Version XM_015288189.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3468bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X49
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288189.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGTAAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT
    GATCCAGATA CAGAACTGTA CCTCACGCAG CCCTTTGCAT GTGGAACCGC ATTTGCTGTC
    AGCGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA
    CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC CGAGGAAAAT
    GCTTTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT
    GCTCAGTTGG CTTTGTATGA TGGGCCATTT GCAGACCTAG GGGATGGAGG CTGCTATGGA
    GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA
    AGGGACGCTC ATCTAAGTAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA
    CCATATGAGT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTCGACCAC
    AATGCTGGCC AGTCCCGGGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC
    AAGAAGAGCT CATCAGTTCA CTCCATCCCA TCAACAGCAA ACCGACCGAA CCGCACCAAG
    ACCAGGGATT CCCGGGAAAA ACAGAAAAAA GAAATGGTTT ACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143675.1
    CDS107..3574
    Translation

    Target ORF information:

    RefSeq Version XM_015288189.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X49, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288189.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCCAT GTTTGCCAGC
    TACGTCCCTG AAATCATAGA GTTAATAGGA AACCGCAAGA AATATGGTGG TTCCTATAGT
    GCGGTTAGTG GAAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGTAAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT
    GATCCAGATA CAGAACTGTA CCTCACGCAG CCCTTTGCAT GTGGAACCGC ATTTGCTGTC
    AGCGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA
    CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC CGAGGAAAAT
    GCTTTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT
    GCTCAGTTGG CTTTGTATGA TGGGCCATTT GCAGACCTAG GGGATGGAGG CTGCTATGGA
    GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA
    AGGGACGCTC ATCTAAGTAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA
    CCATATGAGT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTCGACCAC
    AATGCTGGCC AGTCCCGGGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC
    AAGAAGAGCT CATCAGTTCA CTCCATCCCA TCAACAGCAA ACCGACCGAA CCGCACCAAG
    ACCAGGGATT CCCGGGAAAA ACAGAAAAAA GAAATGGTTT ACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37462
    Clone ID Related Accession (Same CDS sequence) XM_015288190.2 , XM_015288190.1
    Accession Version XM_015288190.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3468bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X50
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288190.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCAAT GTTTGCTCGC
    TACGTGCCAG AAATTGCTGC TCTCATCCTT AATCGGAAGA AATACGGCGG GACTTTTAAC
    TCAACCCGAG GCAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGTAAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT
    GATCCAGATA CAGAACTGTA CCTCACGCAG CCCTTTGCAT GTGGAACCGC ATTTGCTGTC
    AGCGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA
    CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC CGAGGAAAAT
    GCTTTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT
    GCTCAGTTGG CTTTGTATGA TGGGCCATTT GCAGACCTAG GGGATGGAGG CTGCTATGGA
    GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA
    AGGGACGCTC ATCTAAGTAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA
    CCATATGAGT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTCGACCAC
    AATGCTGGCC AGTCCCGGGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC
    AAGAAGAGCT CATCAGTTCA CTCCATCCCA TCAACAGCAA ACCGACCGAA CCGCACCAAG
    ACCAGGGATT CCCGGGAAAA ACAGAAAAAA GAAATGGTTT ACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143676.1
    CDS367..3834
    Translation

    Target ORF information:

    RefSeq Version XM_015288190.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X50, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288190.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCAAT GTTTGCTCGC
    TACGTGCCAG AAATTGCTGC TCTCATCCTT AATCGGAAGA AATACGGCGG GACTTTTAAC
    TCAACCCGAG GCAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGTAAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT
    GATCCAGATA CAGAACTGTA CCTCACGCAG CCCTTTGCAT GTGGAACCGC ATTTGCTGTC
    AGCGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA
    CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC CGAGGAAAAT
    GCTTTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT
    GCTCAGTTGG CTTTGTATGA TGGGCCATTT GCAGACCTAG GGGATGGAGG CTGCTATGGA
    GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA
    AGGGACGCTC ATCTAAGTAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA
    CCATATGAGT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTCGACCAC
    AATGCTGGCC AGTCCCGGGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC
    AAGAAGAGCT CATCAGTTCA CTCCATCCCA TCAACAGCAA ACCGACCGAA CCGCACCAAG
    ACCAGGGATT CCCGGGAAAA ACAGAAAAAA GAAATGGTTT ACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa37462
    Clone ID Related Accession (Same CDS sequence) XM_015288190.2 , XM_015288190.1
    Accession Version XM_015288190.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3468bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1 isoform X43
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCAAT GTTTGCTCGC
    TACGTGCCAG AAATTGCTGC TCTCATCCTT AATCGGAAGA AATACGGCGG GACTTTTAAC
    TCAACCCGAG GCAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGTAAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT
    GATCCAGATA CAGAACTGTA CCTCACGCAG CCCTTTGCAT GTGGAACCGC ATTTGCTGTC
    AGCGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA
    CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC CGAGGAAAAT
    GCTTTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT
    GCTCAGTTGG CTTTGTATGA TGGGCCATTT GCAGACCTAG GGGATGGAGG CTGCTATGGA
    GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA
    AGGGACGCTC ATCTAAGTAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA
    CCATATGAGT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTCGACCAC
    AATGCTGGCC AGTCCCGGGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC
    AAGAAGAGCT CATCAGTTCA CTCCATCCCA TCAACAGCAA ACCGACCGAA CCGCACCAAG
    ACCAGGGATT CCCGGGAAAA ACAGAAAAAA GAAATGGTTT ACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143676.1
    CDS373..3840
    Translation

    Target ORF information:

    RefSeq Version XM_015288190.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant X43, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015288190.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CATTAGAATG 
    AGTAACAATA TCAACGCCAA CAATCTCAAC ACAGACTCGT CCTCCTCCCC CGTCAATGTG
    CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG TGCCCTGCGA TAGCCGCGGA
    CAGCGCATGT GGTGGGCTTT CCTCGCCTCA TCCATGGTCA CTTTCTTTGG GGGACTGTTT
    ATCATTCTGC TGTGGAGGAC CCTCAAGTAC CTGTGGACTG TCTGCTGCCA CTGCGGGGTC
    AAAAACAAGG AGGCCCAGAA GATTAATGGT GGTGGAGATA CGCAGGCAGA TGGGGCCTGC
    AAACCAACAG ATGAAAAAGA GGAGAACGTG GCAGCAGAAG TGGGATGGAT GACCTCCGTG
    AAAGACTGGG CAGGAGTCAT GATATCTGCC CAGACACTAA CTGGCAGAGT ACTTGTTGTC
    TTAGTCTTCG CTCTTAGTAT TGGTGCTCTT GTAATATACT TCATAGATTC ATCAAACCCA
    ATAGAATCCT GCCAGAATTT CTACAAAGAT TTCACATTAC AGATTGACAT GGCTTTCAAT
    GTGTTCTTCC TACTCTACTT CGGCTTGCGT TTCATAGCAG CCAATGACAA GCTATGGTTT
    TGGCTTGAAG TCAACTCAGT AGTAGATTTC TTCACGGTCC CTCCAGTGTT TGTTTCAGTA
    TATTTAAACA GAAGTTGGCT TGGTTTAAGA TTTTTAAGAG CCCTTAGACT GATACAGTTT
    TCAGAAATAT TGCAGTTTCT GAATATCCTT AAAACAAGTA ATTCCATCAA GCTAGTGAAT
    CTGTGCTCTA TATTTATCAG CACATGGCTG ACAGCAGCTG GGTTCATACA TTTGGTGGAG
    AACTCAGGGG ATCCTTGGGA AAATTTCCAA AATAATCAAC AGTTAACATA TTGGGAATGT
    GTTTATTTAC TGATGGTTAC AATGTCCACT GTTGGTTATG GAGATGTTTA TGCAAAAACA
    ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC ATCCTTGGGG GATTGGCAAT GTTTGCTCGC
    TACGTGCCAG AAATTGCTGC TCTCATCCTT AATCGGAAGA AATACGGCGG GACTTTTAAC
    TCAACCCGAG GCAGAAAGCA CATAGTTGTC TGTGGTCACA TAACACTTGA AAGCGTGTCC
    AACTTCCTGA AGGACTTCCT GCACAAGGAT AGGGATGATG TTAACGTAGA AATCGTCTTT
    CTGCATAATA TCTCCCCTAA CCTTGAGCTG GAAGCTCTTT TTAAACGACA CTTCACTCAG
    GTGGAATTTT ATCAGGGTTC AGTCCTTAAT CCCCATGACC TGGCAAGAGT AAAGATAGAG
    TCAGCAGATG CGTGCCTAAT TCTTGCCAAT AAATACTGCG CTGACCCAGA TGCTGAGGAT
    GCCTCCAATA TTATGAGAGT TATTTCAATA AAGAATTATC ATCCGAAGAT AAGAATTATC
    ACTCAGATGT TGCAGTATCA CAATAAGGCT CATCTGTTAA ATATTCCAAG CTGGAATTGG
    AAGGAAGGGG ATGATGCGAT CTGTCTTGCT GAGCTCAAGC TGGGTTTCAT AGCTCAGAGT
    TGCCTGGCAC CAGGCCTTTC AACAATGTTA GCCAACCTCT TTTCCATGAG ATCATTCATA
    AAGATTGAGG AGGATACATG GCAGAAATAC TATCTGGAAG GAGTTGCAAA TGAAATGTAT
    ACAGAATATC TGTCTAGTGC CTTTGTGGGT TTGTCCTTCC CTGCAGTTTG TGAACTGGTG
    TTTGCGAAAC TAAAGCTCTT AATGATAGCC ATAGAATACA AGTCGGAAAA AAGAGAAAGC
    AGTATATTGA TCAATCCTGG AAACCATGTG AAGATTCAAG AAGGTACCTT AGGATTCTTC
    ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG GCATTTTTTT ACTGCAAGGC CTGCCATGAT
    GACATCACAG ATCCCAAAAG GATAAAAAAG TGTGGCTGCA AACGGCTTGA AGATGAGCAG
    CCGTCAACAC TATCACCCAA AAAAAAGCAA CGAAATGGAG GCATGCGAAA TTCACCCAAC
    TCCTCACCCA AACTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA ACAGATTGAC
    AATATGGATG CCAATGTGAA AAAATATGAC TCTACGGGAA TGTTTCATTG GTGTCCAGCC
    AAGGATATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG
    CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA
    GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG
    GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACCCTGC ATAACTTCCC CAAGGTTTCA
    ATCTTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC
    AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGTA TGGATAGGTC ATCTCCAGAC
    AACAGTCCTG TCCACGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT
    ATTATAACAG AGCTAGTAAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT
    GATCCAGATA CAGAACTGTA CCTCACGCAG CCCTTTGCAT GTGGAACCGC ATTTGCTGTC
    AGCGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA
    CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC CGAGGAAAAT
    GCTTTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT
    GCTCAGTTGG CTTTGTATGA TGGGCCATTT GCAGACCTAG GGGATGGAGG CTGCTATGGA
    GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA
    AGGGACGCTC ATCTAAGTAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA
    CCATATGAGT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTCGACCAC
    AATGCTGGCC AGTCCCGGGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC
    AAGAAGAGCT CATCAGTTCA CTCCATCCCA TCAACAGCAA ACCGACCGAA CCGCACCAAG
    ACCAGGGATT CCCGGGAAAA ACAGAAAAAA GAAATGGTTT ACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa27550
    Clone ID Related Accession (Same CDS sequence) NM_204224.1
    Accession Version NM_204224.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3414bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Gallus gallus(chicken)
    Product calcium-activated potassium channel subunit alpha-1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U23821.1. On Jan 18, 2005 this sequence version replaced XM_429228.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U23821.1, AF087663.1 [ECO:0000332] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCTATGG TCACTTTCTT TGGGGGACTG
    TTTATCATTC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG
    GTCAAAAACA AGGAGGCCCA GAAGATTAAT GGTGGTGGAG ATACCAGGGC AGATGGGGCC
    TGCAAACCAA CAGATGAAAA AGAGGAGAAC GTGGCAGCAG AAGTGGGATG GATGACCTCC
    GTGAAAGACT GGGCAGGAGT CATGATATCT GCCCAGACAC TAACTGGCAG AGTACTTGTT
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACAT TACAGATTGA CATGGCTTTC
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG
    TTTTGGCTTG AAGTCAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTTTCA
    GTATATTTAA ACAGAAGTTG GCTTGGTTTA AGATTTTTAA GAGCCCTTAG ACTGATACAG
    TTTTCAGAAA TATTGCAGTT TCTGAATATC CTTAAAACAA GTAATTCCAT CAAGCTAGTG
    AATCTGTGCT CTATATTTAT CAGCACATGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG
    GAGAACTCAG GGGATCCTTG GGAAAATTTC CAAAATAATC AACAGTTAAC ATATTGGGAA
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGTT ATGGAGATGT TTATGCAAAA
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGCGTG
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTTAACAT AGAAATCGTC
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCAAG AGTAAAGATA
    GAGTCAGCAG ATGCGTGCCT AATTCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAG
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGT TAAATATTCC AAGCTGGAAT
    TGGAAGGAAG GGGATGATGC GATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCTCAG
    AGTTCGCTGG CACCAGGCCT TTCAACAATG TTAGCCAACC TCTTTTCCAT GAGATCATTC
    ATAAAGATTG AGGAGGATAC ATGGCAGAAA TACTATCTGG AAGGAGTTGC AAATGAAATG
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA
    AGCAGTATAT TGATCAATCC TGGAAACCAT GTGAAGATTC AAGAAGGTAC CTTAGGATTC
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGCCAT
    GATGACATCA CAGATCCCAA AAGGATAAAA AAGTGTGGCT GCAAACGGCT TGAAGATGAG
    CAGCCGTCAA CACTATCACC CAAAAAAAAG CAACGAAATG GAGGCATGCG AAATTCACCC
    AACTCCTCAC CCAAACTGAT GAGGCATGAC CCCTTGTTAA TTCCTGGCAA TGAACAGATT
    GACAATATGG ATGCCAATGT GAAAAAATAT GACTCTACGG GAATGTTTCA TTGGTGTCCA
    GCCAAGGATA TTGAAAAGGT CATTTTGACT CGAAGTGAAG CAGCGATGAC AGTTTTAAGT
    GGGCATGTAG TCGTTTGCAT ATTTGGGGAT GTTAAATCTG CTTTGATTGG ATTAAGAAAT
    TTAGTGATGC CATTACGAGC CAGCAACTTT CACTACCATG AACTCAAGCA CATTGTGTTT
    GTGGGTTCAC TTGAATACCT GAGAAGGGAG TGGGAGACCC TGCATAACTT CCCCAAGGTT
    TCAATCTTGC CTGGTACACC ATTAAGTCGG GCTGATTTAA GGGCTGTCAA CATCAACCTC
    TGCGACATGT GCGTTATCCT GTCAGCCAAT CAGAATAATA TTGATGATGC TTCGCTGCAG
    GACAAGGAAT GCATCTTGGC GTCACTCAAC ATCAAATCTA TGCAGTTTGA TGACAGCATT
    GGGGTCTTGC AGGCTAATTC CCAAGGCTTT ACACCCCCTG GTATGGATAG GTCATCTCCA
    GACAACAGTC CTGTCCACGG CCTGTTACGT CAACCCTCCA TTACAACAGG AGCCAACATC
    CCTATTATAA CAGAGCTAGT AAATGATTCA AATGTTCAGT TCTTGGACCA AGATGATGAT
    GATGATCCAG ATACAGAACT GTACCTCACG CAGCCCTTTG CATGTGGAAC CGCATTTGCT
    GTCAGCGTGC TGGACTCTCT CATGAGCGCA ACATACTTCA ATGACAATAT CCTCACACTG
    ATACGGACAT TGGTAACAGG AGGAGCCACA CCAGAGCTGG AAGCACTGAT TGCCGAGGAA
    AATGCTTTGA GAGGAGGTTA CAGCACGCCC CAGACACTTG CAAACAGGGA CAGGTGTCGA
    GTTGCTCAGT TGGCTTTGTA TGATGGGCCA TTTGCAGACC TAGGGGATGG AGGCTGCTAT
    GGAGATCTAT TTTGTAAAGC ACTGAAAACA TACAATATGC TTTGCTTTGG AATTTATCGA
    TTAAGGGACG CTCATCTAAG TACTCCCAGC CAATGCACTA AACGTTATGT TATCACAAAC
    CCACCATATG AGTTTGAGCT TGTGCCGACA GACTTGATCT TCTGTTTGAT GCAATTCGAC
    CACAATGCTG GCCAGTCCCG GGCCAGTCTT TCTCATTCCT CCCATTCCTC CTATTCTTCC
    AGCAAGAAGA GCTCATCAGT TCACTCCATC CCATCAACAG CAAACCGACC GAACCGCACC
    AAGACCAGGG ATTCCCGGGA AAAACAGAAG TATGTGCAGG AAGATAGACT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_989555.1
    CDS88..3501
    Translation

    Target ORF information:

    RefSeq Version NM_204224.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_204224.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCTATGG TCACTTTCTT TGGGGGACTG
    TTTATCATTC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG
    GTCAAAAACA AGGAGGCCCA GAAGATTAAT GGTGGTGGAG ATACCAGGGC AGATGGGGCC
    TGCAAACCAA CAGATGAAAA AGAGGAGAAC GTGGCAGCAG AAGTGGGATG GATGACCTCC
    GTGAAAGACT GGGCAGGAGT CATGATATCT GCCCAGACAC TAACTGGCAG AGTACTTGTT
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACAT TACAGATTGA CATGGCTTTC
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG
    TTTTGGCTTG AAGTCAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTTTCA
    GTATATTTAA ACAGAAGTTG GCTTGGTTTA AGATTTTTAA GAGCCCTTAG ACTGATACAG
    TTTTCAGAAA TATTGCAGTT TCTGAATATC CTTAAAACAA GTAATTCCAT CAAGCTAGTG
    AATCTGTGCT CTATATTTAT CAGCACATGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG
    GAGAACTCAG GGGATCCTTG GGAAAATTTC CAAAATAATC AACAGTTAAC ATATTGGGAA
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGTT ATGGAGATGT TTATGCAAAA
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGCGTG
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTTAACAT AGAAATCGTC
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCAAG AGTAAAGATA
    GAGTCAGCAG ATGCGTGCCT AATTCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAG
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGT TAAATATTCC AAGCTGGAAT
    TGGAAGGAAG GGGATGATGC GATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCTCAG
    AGTTCGCTGG CACCAGGCCT TTCAACAATG TTAGCCAACC TCTTTTCCAT GAGATCATTC
    ATAAAGATTG AGGAGGATAC ATGGCAGAAA TACTATCTGG AAGGAGTTGC AAATGAAATG
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA
    AGCAGTATAT TGATCAATCC TGGAAACCAT GTGAAGATTC AAGAAGGTAC CTTAGGATTC
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGCCAT
    GATGACATCA CAGATCCCAA AAGGATAAAA AAGTGTGGCT GCAAACGGCT TGAAGATGAG
    CAGCCGTCAA CACTATCACC CAAAAAAAAG CAACGAAATG GAGGCATGCG AAATTCACCC
    AACTCCTCAC CCAAACTGAT GAGGCATGAC CCCTTGTTAA TTCCTGGCAA TGAACAGATT
    GACAATATGG ATGCCAATGT GAAAAAATAT GACTCTACGG GAATGTTTCA TTGGTGTCCA
    GCCAAGGATA TTGAAAAGGT CATTTTGACT CGAAGTGAAG CAGCGATGAC AGTTTTAAGT
    GGGCATGTAG TCGTTTGCAT ATTTGGGGAT GTTAAATCTG CTTTGATTGG ATTAAGAAAT
    TTAGTGATGC CATTACGAGC CAGCAACTTT CACTACCATG AACTCAAGCA CATTGTGTTT
    GTGGGTTCAC TTGAATACCT GAGAAGGGAG TGGGAGACCC TGCATAACTT CCCCAAGGTT
    TCAATCTTGC CTGGTACACC ATTAAGTCGG GCTGATTTAA GGGCTGTCAA CATCAACCTC
    TGCGACATGT GCGTTATCCT GTCAGCCAAT CAGAATAATA TTGATGATGC TTCGCTGCAG
    GACAAGGAAT GCATCTTGGC GTCACTCAAC ATCAAATCTA TGCAGTTTGA TGACAGCATT
    GGGGTCTTGC AGGCTAATTC CCAAGGCTTT ACACCCCCTG GTATGGATAG GTCATCTCCA
    GACAACAGTC CTGTCCACGG CCTGTTACGT CAACCCTCCA TTACAACAGG AGCCAACATC
    CCTATTATAA CAGAGCTAGT AAATGATTCA AATGTTCAGT TCTTGGACCA AGATGATGAT
    GATGATCCAG ATACAGAACT GTACCTCACG CAGCCCTTTG CATGTGGAAC CGCATTTGCT
    GTCAGCGTGC TGGACTCTCT CATGAGCGCA ACATACTTCA ATGACAATAT CCTCACACTG
    ATACGGACAT TGGTAACAGG AGGAGCCACA CCAGAGCTGG AAGCACTGAT TGCCGAGGAA
    AATGCTTTGA GAGGAGGTTA CAGCACGCCC CAGACACTTG CAAACAGGGA CAGGTGTCGA
    GTTGCTCAGT TGGCTTTGTA TGATGGGCCA TTTGCAGACC TAGGGGATGG AGGCTGCTAT
    GGAGATCTAT TTTGTAAAGC ACTGAAAACA TACAATATGC TTTGCTTTGG AATTTATCGA
    TTAAGGGACG CTCATCTAAG TACTCCCAGC CAATGCACTA AACGTTATGT TATCACAAAC
    CCACCATATG AGTTTGAGCT TGTGCCGACA GACTTGATCT TCTGTTTGAT GCAATTCGAC
    CACAATGCTG GCCAGTCCCG GGCCAGTCTT TCTCATTCCT CCCATTCCTC CTATTCTTCC
    AGCAAGAAGA GCTCATCAGT TCACTCCATC CCATCAACAG CAAACCGACC GAACCGCACC
    AAGACCAGGG ATTCCCGGGA AAAACAGAAG TATGTGCAGG AAGATAGACT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.