gRNAs
The following PFKM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PFKM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OGa28303 | |
| Clone ID Related Accession (Same CDS sequence) | NM_204223.2 | |
| Accession Version | NM_204223.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-01-11 | |
| Organism | Gallus gallus(chicken) | |
| Product | ATP-dependent 6-phosphofructokinase, muscle type | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CO503521.1, AB061205.1, CO503451.1, CO504745.1 and BU309531.1. On Nov 18, 2014 this sequence version replaced NM_204223.1. ##Evidence-Data-START## Transcript exon combination :: AB061205.1 [ECO:0000332] ##Evidence-Data-END## | |
1 | ATGGCGCAGC CCCCGAAGCA TTGCGGGAGC GGGGAGCACA TGGGTGCTGG AAAAGCCATC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_989554.3 |
| CDS | 96..2444 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_204223.2 |
1 | ATGGCGCAGC CCCCGAAGCA TTGCGGGAGC GGGGAGCACA TGGGTGCTGG AAAAGCCATC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa50160 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015273040.1 , XM_015273040.2 | |
| Accession Version | XM_015273040.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-01-03 | |
| Organism | Gallus gallus(chicken) | |
| Product | ATP-dependent 6-phosphofructokinase, muscle type isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_464144.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCGCAGC CCCCGAAGCA TTGCGGGAGC GGGGAGCACA TGGGTGCTGG AAAAGCCATC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015128526.1 |
| CDS | 82..2430 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015273040.1 |
1 | ATGGCGCAGC CCCCGAAGCA TTGCGGGAGC GGGGAGCACA TGGGTGCTGG AAAAGCCATC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa50160 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015273040.1 , XM_015273040.2 | |
| Accession Version | XM_015273040.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-16 | |
| Organism | Gallus gallus(chicken) | |
| Product | ATP-dependent 6-phosphofructokinase, muscle type isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008465.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015273040.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCGCAGC CCCCGAAGCA TTGCGGGAGC GGGGAGCACA TGGGTGCTGG AAAAGCCATC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015128526.1 |
| CDS | 28..2376 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015273040.2 |
1 | ATGGCGCAGC CCCCGAAGCA TTGCGGGAGC GGGGAGCACA TGGGTGCTGG AAAAGCCATC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Two types of phosphofructokinase-1 differentially regulate the glycolytic pathway in insulin-stimulated chicken skeletal muscle. |
A comprehensive collection of chicken cDNAs. |
