ROCK1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	ROCK1
Entrez Gene ID	373970
Full Name	Rho-associated, coiled-coil containing protein kinase 1
General protein information	Preferred Names Rho-associated, coiled-coil containing protein kinase 1 Names rho-associated protein kinase 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001199448.1	NP_001186377.1	rho-associated protein kinase 1
XM_015277931.2	XP_015133417.1	rho-associated protein kinase 1 isoform X1
XM_015277931.1	XP_015133417.1	rho-associated protein kinase 1 isoform X1
XM_015277932.1	XP_015133418.1	rho-associated protein kinase 1 isoform X2
XM_015277932.2	XP_015133418.1	rho-associated protein kinase 1 isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	rock1	NP_001243563.1
Canis lupus familiaris (dog)	ROCK1	XP_005623079.1
Rattus norvegicus (Norway rat)	Rock1	NP_112360.1
Xenopus tropicalis (tropical clawed frog)	rock1	XP_002934180.2
Homo sapiens (human)	ROCK1	NP_005397.1
Pan troglodytes (chimpanzee)	ROCK1	XP_001151982.1
	ROCK1	XP_001091266.1
Bos taurus (cattle)	ROCK1	XP_002697835.2
Mus musculus (house mouse)	Rock1	NP_033097.1
Gallus gallus (chicken)	ROCK1	NP_001186377.1

Gene Ontology
Bibliography

Related articles in PubMed

RHOA-ROCK signalling is necessary for lateralization and differentiation of the developing sinoatrial node.
Vicente-Steijn R, Kelder TP, Tertoolen LG, Wisse LJ, Pijnappels DA, Poelmann RE, Schalij MJ, deRuiter MC, Gittenberger-de Groot AC, Jongbloed MRM
Cardiovascular research113(10)1186-1197(2017 Aug)

Metastasis of aggressive amoeboid sarcoma cells is dependent on Rho/ROCK/MLC signaling.
Kosla J, Pa?kov? D, Plach? J, Tolde O, Bicanov? K, Dvo??k M, R?sel D, Br?bek J
Cell communication and signaling : CCS1151(2013 Jul)

Downregulation of ROCK-I and ROCK-II gene expression in the cadmium-induced ventral body wall defect chick model.
Doi T, Puri P, Bannigan J, Thompson J
Pediatric surgery international24(12)1297-301(2008 Dec)

ROCK1 expression is regulated by TGFbeta3 and ALK2 during valvuloseptal endocardial cushion formation.
Sakabe M, Sakata H, Matsui H, Ikeda K, Yamagishi T, Nakajima Y
Anatomical record (Hoboken, N.J. : 2007)291(7)845-57(2008 Jul)

Rho kinase inhibitor Y27632 affects initial heart myofibrillogenesis in cultured chick blastoderm.
Sakata H, Sakabe M, Matsui H, Kawada N, Nakatani K, Ikeda K, Yamagishi T, Nakajima Y
Developmental dynamics : an official publication of the American Association of Anatomists236(2)461-72(2007 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Metastasis of aggressive amoeboid sarcoma cells is dependent on Rho/ROCK/MLC signaling.
Title: Metastasis of aggressive amoeboid sarcoma cells is dependent on Rho/ROCK/MLC signaling.

the downregulation of ROCK-I and ROCK-II gene expressions during this narrow window of embryogenesis may cause ventral body wall defect by disrupting Wnt non-canonical pathway.
Title: Downregulation of ROCK-I and ROCK-II gene expression in the cadmium-induced ventral body wall defect chick model.

the expression of ROCK1 is up-regulated at the onset of endocardial epithelial-mesenchymal transition and signaling mediated by TGFbeta3/ALK2 together with BMP is involved in the expression of ROCK1
Title: ROCK1 expression is regulated by TGFbeta3 and ALK2 during valvuloseptal endocardial cushion formation.

ROCK1 plays a role in the formation of initial heart myofibrillogenesis by means of actin-myosin assembly, and focal adhesion/costamere and cell-cell adhesion.
Title: Rho kinase inhibitor Y27632 affects initial heart myofibrillogenesis in cultured chick blastoderm.

Rho kinase phosphorylates CRMP-2, and inactivates the ability of CRMP-2 to promote microtubule assembly and Numb-mediated endocytosis, during growth cone collapse
Title: Phosphorylation by Rho kinase regulates CRMP-2 activity in growth cones.

The following ROCK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ROCK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa32475	XM_015277931.2 Latest version!	Gallus gallus Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa32476	XM_015277932.2 Latest version!	Gallus gallus Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa00285	NM_001199448.1 Latest version!	Gallus gallus Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OGa32475	XM_015277931.1	Gallus gallus Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa32476	XM_015277932.1	Gallus gallus Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ROCK1 ( XM_015277931.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133417.1 Gallus gallus Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa32475
Clone ID Related Accession (Same CDS sequence)	XM_015277931.2 , XM_015277931.1
Accession Version	XM_015277931.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	rho-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277931.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACGG GAGAAAGTTT TGAGTCTCGG TTCGAAAAAA TCGACGGGAC TTTAAAGGAT 
CCCAAATCCG AAGTGAACGT GGACTGTTTG CTGGATGGGT TAGATGCTTT GGTATATGAC 
TTGGATTTTC CTGCCTTACG AAAAAACAAA AACATTGATA ACTTCTTAAA CAGGTATAAA 
GACACAGTAA ATAAAATGAG AGATCTGCGG ATGAAAGCTG AAGATTATGA AGTGGTGAAG 
GTGATTGGCA GAGGGGCATT TGGGGAGGTT CAGTTGGTAA GGCATAAATC ATCAAGAAGA 
GTGTATGCTA TGAAACTTCT GAGCAAATTT GAGATGATAA AAAGATCAGA TTCTGCGTTC 
TTCTGGGAAG AAAGGGATAT TATGGCTTTT GCTAATAGTC CCTGGGTTGT TCAGTTGTTT 
TACGCATTCC AAGATGATCG TTACCTTTAC ATGGTCATGG AATACATGCC TGGTGGAGAC 
CTTGTGAATT TAATGAGTAA CTATGATGTT CCTGAGAAAT GGGCAAGATT TTATACTGCT 
GAAGTTGTTC TTGCATTAGA TGCAATTCAC TCAATGGGCT TTATACACAG AGATGTGAAG 
CCTGACAATA TGCTGCTAGA TAAAGCTGGT CATTTAAAAC TAGCAGATTT TGGTACTTGT 
ATGAAAATGA ACAAGGAAGG TATGGTACGA TGTGATACAG CTGTAGGGAC ACCAGACTAT 
ATCTCTCCTG AAGTATTGAA ATCCCAGGGT GGTGATGGTT ACTATGGACG AGAATGTGAC 
TGGTGGTCAG TTGGAGTTTT TTTGTATGAG ATGCTTGTAG GTGACACGCC TTTTTATGCA 
GATTCTTTGG TTGGAACATA CAGTAAGATT ATGAACCACA AGAACTCCCT TACCTTCCCT 
GATGACAACG AGATCTCTAA AGAGGCAAAA AACCTTATTT GTGCCTTTTT AACTGATAGG 
GAAGTGAGAT TAGGACGAAA TGGCGTAGAA GAAATTAAAC GGCATCTTTT CTTCAAAGAT 
GATCAGTGGG CTTGGGAGAC TCTCAGAGAC ACTGTAGCAC CAGTTGTGCC CGACTTAAGT 
AGTGACATAG ACACTAGTAA TTTTGATGAT ATCGATGAAG ACAAAGGAGA GGAAGAAACA 
TTTCCCATCC CAAAAGCGTT TGTGGGCAAC CAGCTTCCTT TTGTTGGATT TACATATTAC 
AGCAACCGTC GGTATTTATC TGTCTCTGCA GAAAATTCCA ATGATAACAG AATGGGCTCC 
AGTATGGATA AAAGTGTGAT GGAAAATATG CAGAAGATGA TCTATGATTT GGAAGAACAG 
CTACATAATG AAATGCAGCT GAAAGATGAA ATGGAGCAAA AGTGCAGGTC TTCAAACATA 
ACGCTAGACA AGATAATGAA AGAACTGGAT GAAGAGGGAA ATCAAAGAAA GAACCTAGAA 
TTAACAGTGT CCCAGATTGA GAAGGAAAAG ATGGCACTGC AGCATAAGAT AAACGACTAC 
CAAAGGAAAA TTGAACAAGA GAGTGAAAAG AGACGGAATG TGGAAAATGA AGTGTCTACG 
CTAAAAGATC AAATGGAAGA CTTGAAAAAA ATCAGCCAAC ATTCACAAAT TACAAATGAG 
AAGATAACGC AGTTACAAAA ACAACTAGAA GAAGCCAATG ATTTGCTACG GACAGAATCA 
GAAACAGCAG TAAGACTGAG GAAGGGTAAC ACAGAAATGA GCAAGTCATT AAGTCAAGTA 
GAATCACTGA ATAGAGAACT GCAGGAAAGG TGTAGGGTTC TAGAAAGCGC AAAACTGCAG 
GTGGAAAAGG ATTATTATCA GCTGCAAGCA GCTTTGGAAT CAGAACGAAG GGACAGAAGT 
CATGGATCTG AAATGATTGG AGAACTACAA GTTCGGATTA CAACTTTACA AGAAGAATTA 
AAAAACCTTA AAAATAATCT TGAAAGAGTG GAAGCAGAAA GGAAACAAGC ACAGGATAGG 
CTTAATCATT CAGAAAAGGA AAAGAACAAT TTGGAGATAG ATTTGAACTA CAAACTCAAG 
TCTTTACAAG ACCGTTTAGA ACAGGAAGTA AATGAACACA AAGTGACTAA AGCTCGATTA 
ACTGACAAAC ATCAGTCAAT AGAGGAGGCA AAATCAGTTG CAATGTGCGA AATGGAAAAA 
AAAGTAAAGG AAGAAAGGGC AGCAAGAGAA AAGGCAGAGA ATCGAATAGT TCAGGCTGAA 
AAACAGTGTT CCATGTTAGA CTTTGACCTG AAGCAGTCTC AGCAGAAAGT GGAACACCTT 
CTTCAGCAAA AGGAAAGACT GGAGGATGAA GTAAAGAATC TGTCACTTCA GCTAGAGCAA 
GAGACAAATA AGCGAATAAT GGCACAGAAT GAATTAAAGG CACAAGCTTT TGAAGCAGAT 
AACTTGAAGG GTTCTGAGAA GCAACTGAAA CAAGAAATCA ATACGTTATT GGAAGCAAAA 
AGATTACTGG AAGTTGAGTT GGCTCAACTT GCTAAGCAGT ACAGAGGAAA TGAAGGTCAA 
ATGCGTGAGC TTCAGGATCA GCTTGAAGCG GAGCAGTATT TTTCGACACT TTACAAAACT 
CAGGTTAAGG AACTGAAGGA AGAAATTGAT GAAAAGAATA AAGAAACTCA AAGAAAAATG 
CAGGAATTGC AAAATGAGAA AGAAACACTC ACTACGCAGC TGGATTTAGC TGAAACCAAA 
GCTGAGTCGG AGCAGTTGGC ACGGGCACTT CTTGAAGAGC AGTATTTTGA GTTGAGTCAG 
GAGAGCAAAA AAGCAGCATC ACGACATAGG CAAGAAATGA CAGATAAGGA CAGCATCATA 
AGAAGGCTGG AAGAAACTAA CAGCACATTA ACCAAAGATG TTGATTTAGT AACAAAAGAA 
AATTCAGAGA TGAGTGAAAA AATGAAGAAA CAGGAGGAAG AATATAAGCT GAAAAAAGAG 
GAAGAAATAA ACAATATTAG AATGCAATAT GAGAAGAGTA TCAACAATGA GAGAACTCTG 
AAGACACAGG CTGTCAACAA ACTGGCTGAG ATAATGAATA GAAAAGATTT TAAAATAGAT 
AGAAAGAAAG CAAGCATGCA GGATTTGAGG AAGAAAGAGA AGGAAAACCG AAAGCTACAA 
TTGGAGCTTA ATCAAGAAAA AGAGAAATTC AATCAGATGG TAGTGAAATA CCAGAAGGAG 
CTTAATGAGA TGCAAGCGCA ACTAGCAGAA GAATCAACAT ACAGGAATGA GCTACAGATG 
CAGCTGGATA GTAAAGAGAG TGACATAGAA CAGCTACGAA GGAAAATTTT GGATCTCCAG 
CAAGGAATGG ATTCCACCAG TGTTTCGAGT CTACAGCCAG ATGAAATTGA TGGAAATCTT 
TCAGAATCAA GGCTGGAAGG TTGGCTTTCA ATACCAAACA AGGGAAACAT AAAGCGACAC 
GGCTGGAAGA AACAGTACGT TGTGGTGAGC AGTAAAAAGA TACTGTTCTA TAATGATGAA 
AAGGACAAGG ACCAATCTAA TCCATCCATG GTACTGGACA TAGATAAGCT GTTCCACGTC 
CGACCTGTAA CTCAAGGAGA TGTATATAGA GCAGAAACTG AAGAAATTCC TAAAATATTC 
CAGATTCTGT ATGCTAATGA GGGGGAATGC AGGAAGGATG TGGAGGTAGA ATCAGTCCAG 
CCTGCAGAGA AGACAAACTT TCTAAATCAT AAAGGCCATG AATTTATTCC AACAATATAC 
CACTTTCCAG CCAACTGTGA GGCCTGTGCC AAACCTCTTT GGCATGTCTT TAAACCCCCA 
GCTGCCCTGG AATGTCGAAG ATGCCATGTG AAGTGCCATA GAGACCACTT GGATAAAAAG 
GAGGAGTTAA TTGCACCGTG CAAAGTGAGT TACGACGTAA CTTCAGCGAG AGATATGTTG 
TTGCTGGCAT CCTGTCAAGA TGAACAGAAG AAATGGGTGA CTCATTTAGT AAAGAAGATC 
CCGAAGAACC CACCGTCTAC TTTTGTTCGT GCTTCTCCCA GAACTATGTC TACAAGATCC 
TCAGCAAACC AGTCATTCCG AAAAGCTGTT AAAAATACTT CTGGAAAAAC GAGGCACCAA 
GTATGGAGGA AGAGGAATAT TGAGCATAAG CAATTTAGAG AGGCACAGCT GTTGGCCAGC 
AAGAGTGCTA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133417.1
CDS	88..4242
Translation	MSTGESFESRFEKIDGTLKDPKSEVNVDCLLDGLDALVYDLDFPALRKNKNIDNFLNRYKDTVNKMRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSSRRVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKAGHLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNEISKEAKNLICAFLTDREVRLGRNGVEEIKRHLFFKDDQWAWETLRDTVAPVVPDLSSDIDTSNFDDIDEDKGEEETFPIPKAFVGNQLPFVGFTYYSNRRYLSVSAENSNDNRMGSSMDKSVMENMQKMIYDLEEQLHNEMQLKDEMEQKCRSSNITLDKIMKELDEEGNQRKNLELTVSQIEKEKMALQHKINDYQRKIEQESEKRRNVENEVSTLKDQMEDLKKISQHSQITNEKITQLQKQLEEANDLLRTESETAVRLRKGNTEMSKSLSQVESLNRELQERCRVLESAKLQVEKDYYQLQAALESERRDRSHGSEMIGELQVRITTLQEELKNLKNNLERVEAERKQAQDRLNHSEKEKNNLEIDLNYKLKSLQDRLEQEVNEHKVTKARLTDKHQSIEEAKSVAMCEMEKKVKEERAAREKAENRIVQAEKQCSMLDFDLKQSQQKVEHLLQQKERLEDEVKNLSLQLEQETNKRIMAQNELKAQAFEADNLKGSEKQLKQEINTLLEAKRLLEVELAQLAKQYRGNEGQMRELQDQLEAEQYFSTLYKTQVKELKEEIDEKNKETQRKMQELQNEKETLTTQLDLAETKAESEQLARALLEEQYFELSQESKKAASRHRQEMTDKDSIIRRLEETNSTLTKDVDLVTKENSEMSEKMKKQEEEYKLKKEEEINNIRMQYEKSINNERTLKTQAVNKLAEIMNRKDFKIDRKKASMQDLRKKEKENRKLQLELNQEKEKFNQMVVKYQKELNEMQAQLAEESTYRNELQMQLDSKESDIEQLRRKILDLQQGMDSTSVSSLQPDEIDGNLSESRLEGWLSIPNKGNIKRHGWKKQYVVVSSKKILFYNDEKDKDQSNPSMVLDIDKLFHVRPVTQGDVYRAETEEIPKIFQILYANEGECRKDVEVESVQPAEKTNFLNHKGHEFIPTIYHFPANCEACAKPLWHVFKPPAALECRRCHVKCHRDHLDKKEELIAPCKVSYDVTSARDMLLLASCQDEQKKWVTHLVKKIPKNPPSTFVRASPRTMSTRSSANQSFRKAVKNTSGKTRHQVWRKRNIEHKQFREAQLLASKSAS

Target ORF information:

RefSeq Version	XM_015277931.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277931.2

ORF Insert Sequence:

ATGTCGACGG GAGAAAGTTT TGAGTCTCGG TTCGAAAAAA TCGACGGGAC TTTAAAGGAT 
CCCAAATCCG AAGTGAACGT GGACTGTTTG CTGGATGGGT TAGATGCTTT GGTATATGAC 
TTGGATTTTC CTGCCTTACG AAAAAACAAA AACATTGATA ACTTCTTAAA CAGGTATAAA 
GACACAGTAA ATAAAATGAG AGATCTGCGG ATGAAAGCTG AAGATTATGA AGTGGTGAAG 
GTGATTGGCA GAGGGGCATT TGGGGAGGTT CAGTTGGTAA GGCATAAATC ATCAAGAAGA 
GTGTATGCTA TGAAACTTCT GAGCAAATTT GAGATGATAA AAAGATCAGA TTCTGCGTTC 
TTCTGGGAAG AAAGGGATAT TATGGCTTTT GCTAATAGTC CCTGGGTTGT TCAGTTGTTT 
TACGCATTCC AAGATGATCG TTACCTTTAC ATGGTCATGG AATACATGCC TGGTGGAGAC 
CTTGTGAATT TAATGAGTAA CTATGATGTT CCTGAGAAAT GGGCAAGATT TTATACTGCT 
GAAGTTGTTC TTGCATTAGA TGCAATTCAC TCAATGGGCT TTATACACAG AGATGTGAAG 
CCTGACAATA TGCTGCTAGA TAAAGCTGGT CATTTAAAAC TAGCAGATTT TGGTACTTGT 
ATGAAAATGA ACAAGGAAGG TATGGTACGA TGTGATACAG CTGTAGGGAC ACCAGACTAT 
ATCTCTCCTG AAGTATTGAA ATCCCAGGGT GGTGATGGTT ACTATGGACG AGAATGTGAC 
TGGTGGTCAG TTGGAGTTTT TTTGTATGAG ATGCTTGTAG GTGACACGCC TTTTTATGCA 
GATTCTTTGG TTGGAACATA CAGTAAGATT ATGAACCACA AGAACTCCCT TACCTTCCCT 
GATGACAACG AGATCTCTAA AGAGGCAAAA AACCTTATTT GTGCCTTTTT AACTGATAGG 
GAAGTGAGAT TAGGACGAAA TGGCGTAGAA GAAATTAAAC GGCATCTTTT CTTCAAAGAT 
GATCAGTGGG CTTGGGAGAC TCTCAGAGAC ACTGTAGCAC CAGTTGTGCC CGACTTAAGT 
AGTGACATAG ACACTAGTAA TTTTGATGAT ATCGATGAAG ACAAAGGAGA GGAAGAAACA 
TTTCCCATCC CAAAAGCGTT TGTGGGCAAC CAGCTTCCTT TTGTTGGATT TACATATTAC 
AGCAACCGTC GGTATTTATC TGTCTCTGCA GAAAATTCCA ATGATAACAG AATGGGCTCC 
AGTATGGATA AAAGTGTGAT GGAAAATATG CAGAAGATGA TCTATGATTT GGAAGAACAG 
CTACATAATG AAATGCAGCT GAAAGATGAA ATGGAGCAAA AGTGCAGGTC TTCAAACATA 
ACGCTAGACA AGATAATGAA AGAACTGGAT GAAGAGGGAA ATCAAAGAAA GAACCTAGAA 
TTAACAGTGT CCCAGATTGA GAAGGAAAAG ATGGCACTGC AGCATAAGAT AAACGACTAC 
CAAAGGAAAA TTGAACAAGA GAGTGAAAAG AGACGGAATG TGGAAAATGA AGTGTCTACG 
CTAAAAGATC AAATGGAAGA CTTGAAAAAA ATCAGCCAAC ATTCACAAAT TACAAATGAG 
AAGATAACGC AGTTACAAAA ACAACTAGAA GAAGCCAATG ATTTGCTACG GACAGAATCA 
GAAACAGCAG TAAGACTGAG GAAGGGTAAC ACAGAAATGA GCAAGTCATT AAGTCAAGTA 
GAATCACTGA ATAGAGAACT GCAGGAAAGG TGTAGGGTTC TAGAAAGCGC AAAACTGCAG 
GTGGAAAAGG ATTATTATCA GCTGCAAGCA GCTTTGGAAT CAGAACGAAG GGACAGAAGT 
CATGGATCTG AAATGATTGG AGAACTACAA GTTCGGATTA CAACTTTACA AGAAGAATTA 
AAAAACCTTA AAAATAATCT TGAAAGAGTG GAAGCAGAAA GGAAACAAGC ACAGGATAGG 
CTTAATCATT CAGAAAAGGA AAAGAACAAT TTGGAGATAG ATTTGAACTA CAAACTCAAG 
TCTTTACAAG ACCGTTTAGA ACAGGAAGTA AATGAACACA AAGTGACTAA AGCTCGATTA 
ACTGACAAAC ATCAGTCAAT AGAGGAGGCA AAATCAGTTG CAATGTGCGA AATGGAAAAA 
AAAGTAAAGG AAGAAAGGGC AGCAAGAGAA AAGGCAGAGA ATCGAATAGT TCAGGCTGAA 
AAACAGTGTT CCATGTTAGA CTTTGACCTG AAGCAGTCTC AGCAGAAAGT GGAACACCTT 
CTTCAGCAAA AGGAAAGACT GGAGGATGAA GTAAAGAATC TGTCACTTCA GCTAGAGCAA 
GAGACAAATA AGCGAATAAT GGCACAGAAT GAATTAAAGG CACAAGCTTT TGAAGCAGAT 
AACTTGAAGG GTTCTGAGAA GCAACTGAAA CAAGAAATCA ATACGTTATT GGAAGCAAAA 
AGATTACTGG AAGTTGAGTT GGCTCAACTT GCTAAGCAGT ACAGAGGAAA TGAAGGTCAA 
ATGCGTGAGC TTCAGGATCA GCTTGAAGCG GAGCAGTATT TTTCGACACT TTACAAAACT 
CAGGTTAAGG AACTGAAGGA AGAAATTGAT GAAAAGAATA AAGAAACTCA AAGAAAAATG 
CAGGAATTGC AAAATGAGAA AGAAACACTC ACTACGCAGC TGGATTTAGC TGAAACCAAA 
GCTGAGTCGG AGCAGTTGGC ACGGGCACTT CTTGAAGAGC AGTATTTTGA GTTGAGTCAG 
GAGAGCAAAA AAGCAGCATC ACGACATAGG CAAGAAATGA CAGATAAGGA CAGCATCATA 
AGAAGGCTGG AAGAAACTAA CAGCACATTA ACCAAAGATG TTGATTTAGT AACAAAAGAA 
AATTCAGAGA TGAGTGAAAA AATGAAGAAA CAGGAGGAAG AATATAAGCT GAAAAAAGAG 
GAAGAAATAA ACAATATTAG AATGCAATAT GAGAAGAGTA TCAACAATGA GAGAACTCTG 
AAGACACAGG CTGTCAACAA ACTGGCTGAG ATAATGAATA GAAAAGATTT TAAAATAGAT 
AGAAAGAAAG CAAGCATGCA GGATTTGAGG AAGAAAGAGA AGGAAAACCG AAAGCTACAA 
TTGGAGCTTA ATCAAGAAAA AGAGAAATTC AATCAGATGG TAGTGAAATA CCAGAAGGAG 
CTTAATGAGA TGCAAGCGCA ACTAGCAGAA GAATCAACAT ACAGGAATGA GCTACAGATG 
CAGCTGGATA GTAAAGAGAG TGACATAGAA CAGCTACGAA GGAAAATTTT GGATCTCCAG 
CAAGGAATGG ATTCCACCAG TGTTTCGAGT CTACAGCCAG ATGAAATTGA TGGAAATCTT 
TCAGAATCAA GGCTGGAAGG TTGGCTTTCA ATACCAAACA AGGGAAACAT AAAGCGACAC 
GGCTGGAAGA AACAGTACGT TGTGGTGAGC AGTAAAAAGA TACTGTTCTA TAATGATGAA 
AAGGACAAGG ACCAATCTAA TCCATCCATG GTACTGGACA TAGATAAGCT GTTCCACGTC 
CGACCTGTAA CTCAAGGAGA TGTATATAGA GCAGAAACTG AAGAAATTCC TAAAATATTC 
CAGATTCTGT ATGCTAATGA GGGGGAATGC AGGAAGGATG TGGAGGTAGA ATCAGTCCAG 
CCTGCAGAGA AGACAAACTT TCTAAATCAT AAAGGCCATG AATTTATTCC AACAATATAC 
CACTTTCCAG CCAACTGTGA GGCCTGTGCC AAACCTCTTT GGCATGTCTT TAAACCCCCA 
GCTGCCCTGG AATGTCGAAG ATGCCATGTG AAGTGCCATA GAGACCACTT GGATAAAAAG 
GAGGAGTTAA TTGCACCGTG CAAAGTGAGT TACGACGTAA CTTCAGCGAG AGATATGTTG 
TTGCTGGCAT CCTGTCAAGA TGAACAGAAG AAATGGGTGA CTCATTTAGT AAAGAAGATC 
CCGAAGAACC CACCGTCTAC TTTTGTTCGT GCTTCTCCCA GAACTATGTC TACAAGATCC 
TCAGCAAACC AGTCATTCCG AAAAGCTGTT AAAAATACTT CTGGAAAAAC GAGGCACCAA 
GTATGGAGGA AGAGGAATAT TGAGCATAAG CAATTTAGAG AGGCACAGCT GTTGGCCAGC 
AAGAGTGCTA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK1 ( XM_015277931.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133417.1 Gallus gallus Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa32475
Clone ID Related Accession (Same CDS sequence)	XM_015277931.2 , XM_015277931.1
Accession Version	XM_015277931.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	rho-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACGG GAGAAAGTTT TGAGTCTCGG TTCGAAAAAA TCGACGGGAC TTTAAAGGAT 
CCCAAATCCG AAGTGAACGT GGACTGTTTG CTGGATGGGT TAGATGCTTT GGTATATGAC 
TTGGATTTTC CTGCCTTACG AAAAAACAAA AACATTGATA ACTTCTTAAA CAGGTATAAA 
GACACAGTAA ATAAAATGAG AGATCTGCGG ATGAAAGCTG AAGATTATGA AGTGGTGAAG 
GTGATTGGCA GAGGGGCATT TGGGGAGGTT CAGTTGGTAA GGCATAAATC ATCAAGAAGA 
GTGTATGCTA TGAAACTTCT GAGCAAATTT GAGATGATAA AAAGATCAGA TTCTGCGTTC 
TTCTGGGAAG AAAGGGATAT TATGGCTTTT GCTAATAGTC CCTGGGTTGT TCAGTTGTTT 
TACGCATTCC AAGATGATCG TTACCTTTAC ATGGTCATGG AATACATGCC TGGTGGAGAC 
CTTGTGAATT TAATGAGTAA CTATGATGTT CCTGAGAAAT GGGCAAGATT TTATACTGCT 
GAAGTTGTTC TTGCATTAGA TGCAATTCAC TCAATGGGCT TTATACACAG AGATGTGAAG 
CCTGACAATA TGCTGCTAGA TAAAGCTGGT CATTTAAAAC TAGCAGATTT TGGTACTTGT 
ATGAAAATGA ACAAGGAAGG TATGGTACGA TGTGATACAG CTGTAGGGAC ACCAGACTAT 
ATCTCTCCTG AAGTATTGAA ATCCCAGGGT GGTGATGGTT ACTATGGACG AGAATGTGAC 
TGGTGGTCAG TTGGAGTTTT TTTGTATGAG ATGCTTGTAG GTGACACGCC TTTTTATGCA 
GATTCTTTGG TTGGAACATA CAGTAAGATT ATGAACCACA AGAACTCCCT TACCTTCCCT 
GATGACAACG AGATCTCTAA AGAGGCAAAA AACCTTATTT GTGCCTTTTT AACTGATAGG 
GAAGTGAGAT TAGGACGAAA TGGCGTAGAA GAAATTAAAC GGCATCTTTT CTTCAAAGAT 
GATCAGTGGG CTTGGGAGAC TCTCAGAGAC ACTGTAGCAC CAGTTGTGCC CGACTTAAGT 
AGTGACATAG ACACTAGTAA TTTTGATGAT ATCGATGAAG ACAAAGGAGA GGAAGAAACA 
TTTCCCATCC CAAAAGCGTT TGTGGGCAAC CAGCTTCCTT TTGTTGGATT TACATATTAC 
AGCAACCGTC GGTATTTATC TGTCTCTGCA GAAAATTCCA ATGATAACAG AATGGGCTCC 
AGTATGGATA AAAGTGTGAT GGAAAATATG CAGAAGATGA TCTATGATTT GGAAGAACAG 
CTACATAATG AAATGCAGCT GAAAGATGAA ATGGAGCAAA AGTGCAGGTC TTCAAACATA 
ACGCTAGACA AGATAATGAA AGAACTGGAT GAAGAGGGAA ATCAAAGAAA GAACCTAGAA 
TTAACAGTGT CCCAGATTGA GAAGGAAAAG ATGGCACTGC AGCATAAGAT AAACGACTAC 
CAAAGGAAAA TTGAACAAGA GAGTGAAAAG AGACGGAATG TGGAAAATGA AGTGTCTACG 
CTAAAAGATC AAATGGAAGA CTTGAAAAAA ATCAGCCAAC ATTCACAAAT TACAAATGAG 
AAGATAACGC AGTTACAAAA ACAACTAGAA GAAGCCAATG ATTTGCTACG GACAGAATCA 
GAAACAGCAG TAAGACTGAG GAAGGGTAAC ACAGAAATGA GCAAGTCATT AAGTCAAGTA 
GAATCACTGA ATAGAGAACT GCAGGAAAGG TGTAGGGTTC TAGAAAGCGC AAAACTGCAG 
GTGGAAAAGG ATTATTATCA GCTGCAAGCA GCTTTGGAAT CAGAACGAAG GGACAGAAGT 
CATGGATCTG AAATGATTGG AGAACTACAA GTTCGGATTA CAACTTTACA AGAAGAATTA 
AAAAACCTTA AAAATAATCT TGAAAGAGTG GAAGCAGAAA GGAAACAAGC ACAGGATAGG 
CTTAATCATT CAGAAAAGGA AAAGAACAAT TTGGAGATAG ATTTGAACTA CAAACTCAAG 
TCTTTACAAG ACCGTTTAGA ACAGGAAGTA AATGAACACA AAGTGACTAA AGCTCGATTA 
ACTGACAAAC ATCAGTCAAT AGAGGAGGCA AAATCAGTTG CAATGTGCGA AATGGAAAAA 
AAAGTAAAGG AAGAAAGGGC AGCAAGAGAA AAGGCAGAGA ATCGAATAGT TCAGGCTGAA 
AAACAGTGTT CCATGTTAGA CTTTGACCTG AAGCAGTCTC AGCAGAAAGT GGAACACCTT 
CTTCAGCAAA AGGAAAGACT GGAGGATGAA GTAAAGAATC TGTCACTTCA GCTAGAGCAA 
GAGACAAATA AGCGAATAAT GGCACAGAAT GAATTAAAGG CACAAGCTTT TGAAGCAGAT 
AACTTGAAGG GTTCTGAGAA GCAACTGAAA CAAGAAATCA ATACGTTATT GGAAGCAAAA 
AGATTACTGG AAGTTGAGTT GGCTCAACTT GCTAAGCAGT ACAGAGGAAA TGAAGGTCAA 
ATGCGTGAGC TTCAGGATCA GCTTGAAGCG GAGCAGTATT TTTCGACACT TTACAAAACT 
CAGGTTAAGG AACTGAAGGA AGAAATTGAT GAAAAGAATA AAGAAACTCA AAGAAAAATG 
CAGGAATTGC AAAATGAGAA AGAAACACTC ACTACGCAGC TGGATTTAGC TGAAACCAAA 
GCTGAGTCGG AGCAGTTGGC ACGGGCACTT CTTGAAGAGC AGTATTTTGA GTTGAGTCAG 
GAGAGCAAAA AAGCAGCATC ACGACATAGG CAAGAAATGA CAGATAAGGA CAGCATCATA 
AGAAGGCTGG AAGAAACTAA CAGCACATTA ACCAAAGATG TTGATTTAGT AACAAAAGAA 
AATTCAGAGA TGAGTGAAAA AATGAAGAAA CAGGAGGAAG AATATAAGCT GAAAAAAGAG 
GAAGAAATAA ACAATATTAG AATGCAATAT GAGAAGAGTA TCAACAATGA GAGAACTCTG 
AAGACACAGG CTGTCAACAA ACTGGCTGAG ATAATGAATA GAAAAGATTT TAAAATAGAT 
AGAAAGAAAG CAAGCATGCA GGATTTGAGG AAGAAAGAGA AGGAAAACCG AAAGCTACAA 
TTGGAGCTTA ATCAAGAAAA AGAGAAATTC AATCAGATGG TAGTGAAATA CCAGAAGGAG 
CTTAATGAGA TGCAAGCGCA ACTAGCAGAA GAATCAACAT ACAGGAATGA GCTACAGATG 
CAGCTGGATA GTAAAGAGAG TGACATAGAA CAGCTACGAA GGAAAATTTT GGATCTCCAG 
CAAGGAATGG ATTCCACCAG TGTTTCGAGT CTACAGCCAG ATGAAATTGA TGGAAATCTT 
TCAGAATCAA GGCTGGAAGG TTGGCTTTCA ATACCAAACA AGGGAAACAT AAAGCGACAC 
GGCTGGAAGA AACAGTACGT TGTGGTGAGC AGTAAAAAGA TACTGTTCTA TAATGATGAA 
AAGGACAAGG ACCAATCTAA TCCATCCATG GTACTGGACA TAGATAAGCT GTTCCACGTC 
CGACCTGTAA CTCAAGGAGA TGTATATAGA GCAGAAACTG AAGAAATTCC TAAAATATTC 
CAGATTCTGT ATGCTAATGA GGGGGAATGC AGGAAGGATG TGGAGGTAGA ATCAGTCCAG 
CCTGCAGAGA AGACAAACTT TCTAAATCAT AAAGGCCATG AATTTATTCC AACAATATAC 
CACTTTCCAG CCAACTGTGA GGCCTGTGCC AAACCTCTTT GGCATGTCTT TAAACCCCCA 
GCTGCCCTGG AATGTCGAAG ATGCCATGTG AAGTGCCATA GAGACCACTT GGATAAAAAG 
GAGGAGTTAA TTGCACCGTG CAAAGTGAGT TACGACGTAA CTTCAGCGAG AGATATGTTG 
TTGCTGGCAT CCTGTCAAGA TGAACAGAAG AAATGGGTGA CTCATTTAGT AAAGAAGATC 
CCGAAGAACC CACCGTCTAC TTTTGTTCGT GCTTCTCCCA GAACTATGTC TACAAGATCC 
TCAGCAAACC AGTCATTCCG AAAAGCTGTT AAAAATACTT CTGGAAAAAC GAGGCACCAA 
GTATGGAGGA AGAGGAATAT TGAGCATAAG CAATTTAGAG AGGCACAGCT GTTGGCCAGC 
AAGAGTGCTA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133417.1
CDS	831..4985
Translation	MSTGESFESRFEKIDGTLKDPKSEVNVDCLLDGLDALVYDLDFPALRKNKNIDNFLNRYKDTVNKMRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSSRRVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKAGHLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNEISKEAKNLICAFLTDREVRLGRNGVEEIKRHLFFKDDQWAWETLRDTVAPVVPDLSSDIDTSNFDDIDEDKGEEETFPIPKAFVGNQLPFVGFTYYSNRRYLSVSAENSNDNRMGSSMDKSVMENMQKMIYDLEEQLHNEMQLKDEMEQKCRSSNITLDKIMKELDEEGNQRKNLELTVSQIEKEKMALQHKINDYQRKIEQESEKRRNVENEVSTLKDQMEDLKKISQHSQITNEKITQLQKQLEEANDLLRTESETAVRLRKGNTEMSKSLSQVESLNRELQERCRVLESAKLQVEKDYYQLQAALESERRDRSHGSEMIGELQVRITTLQEELKNLKNNLERVEAERKQAQDRLNHSEKEKNNLEIDLNYKLKSLQDRLEQEVNEHKVTKARLTDKHQSIEEAKSVAMCEMEKKVKEERAAREKAENRIVQAEKQCSMLDFDLKQSQQKVEHLLQQKERLEDEVKNLSLQLEQETNKRIMAQNELKAQAFEADNLKGSEKQLKQEINTLLEAKRLLEVELAQLAKQYRGNEGQMRELQDQLEAEQYFSTLYKTQVKELKEEIDEKNKETQRKMQELQNEKETLTTQLDLAETKAESEQLARALLEEQYFELSQESKKAASRHRQEMTDKDSIIRRLEETNSTLTKDVDLVTKENSEMSEKMKKQEEEYKLKKEEEINNIRMQYEKSINNERTLKTQAVNKLAEIMNRKDFKIDRKKASMQDLRKKEKENRKLQLELNQEKEKFNQMVVKYQKELNEMQAQLAEESTYRNELQMQLDSKESDIEQLRRKILDLQQGMDSTSVSSLQPDEIDGNLSESRLEGWLSIPNKGNIKRHGWKKQYVVVSSKKILFYNDEKDKDQSNPSMVLDIDKLFHVRPVTQGDVYRAETEEIPKIFQILYANEGECRKDVEVESVQPAEKTNFLNHKGHEFIPTIYHFPANCEACAKPLWHVFKPPAALECRRCHVKCHRDHLDKKEELIAPCKVSYDVTSARDMLLLASCQDEQKKWVTHLVKKIPKNPPSTFVRASPRTMSTRSSANQSFRKAVKNTSGKTRHQVWRKRNIEHKQFREAQLLASKSAS

Target ORF information:

RefSeq Version	XM_015277931.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277931.1

ORF Insert Sequence:

ATGTCGACGG GAGAAAGTTT TGAGTCTCGG TTCGAAAAAA TCGACGGGAC TTTAAAGGAT 
CCCAAATCCG AAGTGAACGT GGACTGTTTG CTGGATGGGT TAGATGCTTT GGTATATGAC 
TTGGATTTTC CTGCCTTACG AAAAAACAAA AACATTGATA ACTTCTTAAA CAGGTATAAA 
GACACAGTAA ATAAAATGAG AGATCTGCGG ATGAAAGCTG AAGATTATGA AGTGGTGAAG 
GTGATTGGCA GAGGGGCATT TGGGGAGGTT CAGTTGGTAA GGCATAAATC ATCAAGAAGA 
GTGTATGCTA TGAAACTTCT GAGCAAATTT GAGATGATAA AAAGATCAGA TTCTGCGTTC 
TTCTGGGAAG AAAGGGATAT TATGGCTTTT GCTAATAGTC CCTGGGTTGT TCAGTTGTTT 
TACGCATTCC AAGATGATCG TTACCTTTAC ATGGTCATGG AATACATGCC TGGTGGAGAC 
CTTGTGAATT TAATGAGTAA CTATGATGTT CCTGAGAAAT GGGCAAGATT TTATACTGCT 
GAAGTTGTTC TTGCATTAGA TGCAATTCAC TCAATGGGCT TTATACACAG AGATGTGAAG 
CCTGACAATA TGCTGCTAGA TAAAGCTGGT CATTTAAAAC TAGCAGATTT TGGTACTTGT 
ATGAAAATGA ACAAGGAAGG TATGGTACGA TGTGATACAG CTGTAGGGAC ACCAGACTAT 
ATCTCTCCTG AAGTATTGAA ATCCCAGGGT GGTGATGGTT ACTATGGACG AGAATGTGAC 
TGGTGGTCAG TTGGAGTTTT TTTGTATGAG ATGCTTGTAG GTGACACGCC TTTTTATGCA 
GATTCTTTGG TTGGAACATA CAGTAAGATT ATGAACCACA AGAACTCCCT TACCTTCCCT 
GATGACAACG AGATCTCTAA AGAGGCAAAA AACCTTATTT GTGCCTTTTT AACTGATAGG 
GAAGTGAGAT TAGGACGAAA TGGCGTAGAA GAAATTAAAC GGCATCTTTT CTTCAAAGAT 
GATCAGTGGG CTTGGGAGAC TCTCAGAGAC ACTGTAGCAC CAGTTGTGCC CGACTTAAGT 
AGTGACATAG ACACTAGTAA TTTTGATGAT ATCGATGAAG ACAAAGGAGA GGAAGAAACA 
TTTCCCATCC CAAAAGCGTT TGTGGGCAAC CAGCTTCCTT TTGTTGGATT TACATATTAC 
AGCAACCGTC GGTATTTATC TGTCTCTGCA GAAAATTCCA ATGATAACAG AATGGGCTCC 
AGTATGGATA AAAGTGTGAT GGAAAATATG CAGAAGATGA TCTATGATTT GGAAGAACAG 
CTACATAATG AAATGCAGCT GAAAGATGAA ATGGAGCAAA AGTGCAGGTC TTCAAACATA 
ACGCTAGACA AGATAATGAA AGAACTGGAT GAAGAGGGAA ATCAAAGAAA GAACCTAGAA 
TTAACAGTGT CCCAGATTGA GAAGGAAAAG ATGGCACTGC AGCATAAGAT AAACGACTAC 
CAAAGGAAAA TTGAACAAGA GAGTGAAAAG AGACGGAATG TGGAAAATGA AGTGTCTACG 
CTAAAAGATC AAATGGAAGA CTTGAAAAAA ATCAGCCAAC ATTCACAAAT TACAAATGAG 
AAGATAACGC AGTTACAAAA ACAACTAGAA GAAGCCAATG ATTTGCTACG GACAGAATCA 
GAAACAGCAG TAAGACTGAG GAAGGGTAAC ACAGAAATGA GCAAGTCATT AAGTCAAGTA 
GAATCACTGA ATAGAGAACT GCAGGAAAGG TGTAGGGTTC TAGAAAGCGC AAAACTGCAG 
GTGGAAAAGG ATTATTATCA GCTGCAAGCA GCTTTGGAAT CAGAACGAAG GGACAGAAGT 
CATGGATCTG AAATGATTGG AGAACTACAA GTTCGGATTA CAACTTTACA AGAAGAATTA 
AAAAACCTTA AAAATAATCT TGAAAGAGTG GAAGCAGAAA GGAAACAAGC ACAGGATAGG 
CTTAATCATT CAGAAAAGGA AAAGAACAAT TTGGAGATAG ATTTGAACTA CAAACTCAAG 
TCTTTACAAG ACCGTTTAGA ACAGGAAGTA AATGAACACA AAGTGACTAA AGCTCGATTA 
ACTGACAAAC ATCAGTCAAT AGAGGAGGCA AAATCAGTTG CAATGTGCGA AATGGAAAAA 
AAAGTAAAGG AAGAAAGGGC AGCAAGAGAA AAGGCAGAGA ATCGAATAGT TCAGGCTGAA 
AAACAGTGTT CCATGTTAGA CTTTGACCTG AAGCAGTCTC AGCAGAAAGT GGAACACCTT 
CTTCAGCAAA AGGAAAGACT GGAGGATGAA GTAAAGAATC TGTCACTTCA GCTAGAGCAA 
GAGACAAATA AGCGAATAAT GGCACAGAAT GAATTAAAGG CACAAGCTTT TGAAGCAGAT 
AACTTGAAGG GTTCTGAGAA GCAACTGAAA CAAGAAATCA ATACGTTATT GGAAGCAAAA 
AGATTACTGG AAGTTGAGTT GGCTCAACTT GCTAAGCAGT ACAGAGGAAA TGAAGGTCAA 
ATGCGTGAGC TTCAGGATCA GCTTGAAGCG GAGCAGTATT TTTCGACACT TTACAAAACT 
CAGGTTAAGG AACTGAAGGA AGAAATTGAT GAAAAGAATA AAGAAACTCA AAGAAAAATG 
CAGGAATTGC AAAATGAGAA AGAAACACTC ACTACGCAGC TGGATTTAGC TGAAACCAAA 
GCTGAGTCGG AGCAGTTGGC ACGGGCACTT CTTGAAGAGC AGTATTTTGA GTTGAGTCAG 
GAGAGCAAAA AAGCAGCATC ACGACATAGG CAAGAAATGA CAGATAAGGA CAGCATCATA 
AGAAGGCTGG AAGAAACTAA CAGCACATTA ACCAAAGATG TTGATTTAGT AACAAAAGAA 
AATTCAGAGA TGAGTGAAAA AATGAAGAAA CAGGAGGAAG AATATAAGCT GAAAAAAGAG 
GAAGAAATAA ACAATATTAG AATGCAATAT GAGAAGAGTA TCAACAATGA GAGAACTCTG 
AAGACACAGG CTGTCAACAA ACTGGCTGAG ATAATGAATA GAAAAGATTT TAAAATAGAT 
AGAAAGAAAG CAAGCATGCA GGATTTGAGG AAGAAAGAGA AGGAAAACCG AAAGCTACAA 
TTGGAGCTTA ATCAAGAAAA AGAGAAATTC AATCAGATGG TAGTGAAATA CCAGAAGGAG 
CTTAATGAGA TGCAAGCGCA ACTAGCAGAA GAATCAACAT ACAGGAATGA GCTACAGATG 
CAGCTGGATA GTAAAGAGAG TGACATAGAA CAGCTACGAA GGAAAATTTT GGATCTCCAG 
CAAGGAATGG ATTCCACCAG TGTTTCGAGT CTACAGCCAG ATGAAATTGA TGGAAATCTT 
TCAGAATCAA GGCTGGAAGG TTGGCTTTCA ATACCAAACA AGGGAAACAT AAAGCGACAC 
GGCTGGAAGA AACAGTACGT TGTGGTGAGC AGTAAAAAGA TACTGTTCTA TAATGATGAA 
AAGGACAAGG ACCAATCTAA TCCATCCATG GTACTGGACA TAGATAAGCT GTTCCACGTC 
CGACCTGTAA CTCAAGGAGA TGTATATAGA GCAGAAACTG AAGAAATTCC TAAAATATTC 
CAGATTCTGT ATGCTAATGA GGGGGAATGC AGGAAGGATG TGGAGGTAGA ATCAGTCCAG 
CCTGCAGAGA AGACAAACTT TCTAAATCAT AAAGGCCATG AATTTATTCC AACAATATAC 
CACTTTCCAG CCAACTGTGA GGCCTGTGCC AAACCTCTTT GGCATGTCTT TAAACCCCCA 
GCTGCCCTGG AATGTCGAAG ATGCCATGTG AAGTGCCATA GAGACCACTT GGATAAAAAG 
GAGGAGTTAA TTGCACCGTG CAAAGTGAGT TACGACGTAA CTTCAGCGAG AGATATGTTG 
TTGCTGGCAT CCTGTCAAGA TGAACAGAAG AAATGGGTGA CTCATTTAGT AAAGAAGATC 
CCGAAGAACC CACCGTCTAC TTTTGTTCGT GCTTCTCCCA GAACTATGTC TACAAGATCC 
TCAGCAAACC AGTCATTCCG AAAAGCTGTT AAAAATACTT CTGGAAAAAC GAGGCACCAA 
GTATGGAGGA AGAGGAATAT TGAGCATAAG CAATTTAGAG AGGCACAGCT GTTGGCCAGC 
AAGAGTGCTA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK1 ( XM_015277932.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133418.1 Gallus gallus Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), transcript variant X2, mRNA.

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CloneID	OGa32476
Clone ID Related Accession (Same CDS sequence)	XM_015277932.1 , XM_015277932.2
Accession Version	XM_015277932.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	rho-associated protein kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACGG GAGAAAGTTT TGAGTCTCGG TTCGAAAAAA TCGACGGGAC TTTAAAGGAT 
CCCAAATCCG AAGTGAACGT GGACTGTTTG CTGGATGGGT TAGATGCTTT GGTATATGAC 
TTGGATTTTC CTGCCTTACG AAAAAACAAA AACATTGATA ACTTCTTAAA CAGGTATAAA 
GACACAGTAA ATAAAATGAG AGATCTGCGG ATGAAAGCTG AAGATTATGA AGTGGTGAAG 
GTGATTGGCA GAGGGGCATT TGGGGAGGTT CAGTTGGTAA GGCATAAATC ATCAAGAAGA 
GTGTATGCTA TGAAACTTCT GAGCAAATTT GAGATGATAA AAAGATCAGA TTCTGCGTTC 
TTCTGGGAAG AAAGGGATAT TATGGCTTTT GCTAATAGTC CCTGGGTTGT TCAGTTGTTT 
TACGCATTCC AAGATGATCG TTACCTTTAC ATGGTCATGG AATACATGCC TGGTGGAGAC 
CTTGTGAATT TAATGAGTAA CTATGATGTT CCTGAGAAAT GGGCAAGATT TTATACTGCT 
GAAGTTGTTC TTGCATTAGA TGCAATTCAC TCAATGGGCT TTATACACAG AGATGTGAAG 
CCTGACAATA TGCTGCTAGA TAAAGCTGGT CATTTAAAAC TAGCAGATTT TGGTACTTGT 
ATGAAAATGA ACAAGGAAGG TATGGTACGA TGTGATACAG CTGTAGGGAC ACCAGACTAT 
ATCTCTCCTG AAGTATTGAA ATCCCAGGGT GGTGATGGTT ACTATGGACG AGAATGTGAC 
TGGTGGTCAG TTGGAGTTTT TTTGTATGAG ATGCTTGTAG GTGACACGCC TTTTTATGCA 
GATTCTTTGG TTGGAACATA CAGTAAGATT ATGAACCACA AGAACTCCCT TACCTTCCCT 
GATGACAACG AGATCTCTAA AGAGGCAAAA AACCTTATTT GTGCCTTTTT AACTGATAGG 
GAAGTGAGAT TAGGACGAAA TGGCGTAGAA GAAATTAAAC GGCATCTTTT CTTCAAAGAT 
GATCAGTGGG CTTGGGAGAC TCTCAGAGAC ACTGTAGCAC CAGTTGTGCC CGACTTAAGT 
AGTGACATAG ACACTAGTAA TTTTGATGAT ATCGATGAAG ACAAAGGAGA GGAAGAAACA 
TTTCCCATCC CAAAAGCGTT TGTGGGCAAC CAGCTTCCTT TTGTTGGATT TACATATTAC 
AGCAACCGTC GGTATTTATC TGTCTCTGCA GAAAATTCCA ATGATAACAG AATGGGCTCC 
AGTATGGATA AAAGTGTGAT GGAAAATATG CAGAAGATGA TCTATGATTT GGAAGAACAG 
CTACATAATG AAATGCAGCT GAAAGATGAA ATGGAGCAAA AGTGCAGGTC TTCAAACATA 
ACGCTAGACA AGATAATGAA AGAACTGGAT GAAGAGGGAA ATCAAAGAAA GAACCTAGAA 
TTAACAGTGT CCCAGATTGA GAAGGAAAAG ATGGCACTGC AGCATAAGAT AAACGACTAC 
CAAAGGAAAA TTGAACAAGA GAGTGAAAAG AGACGGAATG TGGAAAATGA AGTGTCTACG 
CTAAAAGATC AAATGGAAGA CTTGAAAAAA ATCAGCCAAC ATTCACAAAT TACAAATGAG 
AAGATAACGC AGTTACAAAA ACAACTAGAA GAAGCCAATG ATTTGCTACG GACAGAATCA 
GAAACAGCAG TAAGACTGAG GAAGGGTAAC ACAGAAATGA GCAAGTCATT AAGTCAAGTA 
GAATCACTGA ATAGAGAACT GCAGGAAAGG TGTAGGGTTC TAGAAAGCGC AAAACTGCAG 
GTGGAAAAGG ATTATTATCA GCTGCAAGCA GCTTTGGAAT CAGAACGAAG GGACAGAAGT 
CATGGATCTG AAATGATTGG AGAACTACAA GTTCGGATTA CAACTTTACA AGAAGAATTA 
AAAAACCTTA AAAATAATCT TGAAAGAGTG GAAGCAGAAA GGAAACAAGC ACAGGATAGG 
CTTAATCATT CAGAAAAGGA AAAGAACAAT TTGGAGATAG ATTTGAACTA CAAACTCAAG 
TCTTTACAAG ACCGTTTAGA ACAGGAAGTA AATGAACACA AAGTGACTAA AGCTCGATTA 
ACTGACAAAC ATCAGTCAAT AGAGGAGGCA AAATCAGTTG CAATGTGCGA AATGGAAAAA 
AAAGTAAAGG AAGAAAGGGC AGCAAGAGAA AAGGCAGAGA ATCGAATAGT TCAGGCTGAA 
AAACAGTGTT CCATGTTAGA CTTTGACCTG AAGCAGTCTC AGCAGAAAGT GGAACACCTT 
CTTCAGCAAA AGGAAAGACT GGAGGATGAA GTAAAGAATC TGTCACTTCA GCTAGAGCAA 
GAGACAAATA AGCGAATAAT GGCACAGAAT GAATTAAAGG CACAAGCTTT TGAAGCAGAT 
AACTTGAAGG GTTCTGAGAA GCAACTGAAA CAAGAAATCA ATACGTTATT GGAAGCAAAA 
AGATTACTGG AAGTTGAGTT GGCTCAACTT GCTAAGCAGT ACAGAGGAAA TGAAGGTCAA 
ATGCGTGAGC TTCAGGATCA GCTTGAAGCG GAGCAGTATT TTTCGACACT TTACAAAACT 
CAGGTTAAGG AACTGAAGGA AGAAATTGAT GAAAAGAATA AAGAAACTCA AAGAAAAATG 
CAGGAATTGC AAAATGAGAA AGAAACACTC ACTACGCAGC TGGATTTAGC TGAAACCAAA 
GCTGAGTCGG AGCAGTTGGC ACGGGCACTT CTTGAAGAGC AGTATTTTGA GTTGAGTCAG 
GAGAGCAAAA AAGCAGCATC ACGACATAGG CAAGAAATGA CAGATAAGGA CAGCATCATA 
AGAAGGCTGG AAGAAACTAA CAGCACATTA ACCAAAGATG TTGATTTAGT AACAAAAGAA 
AATTCAGAGA TGAGTGAAAA AATGAAGAAA CAGGAGGAAG AATATAAGCT GAAAAAAGAG 
GAAGAAATAA ACAATATTAG AATGCAATAT GAGAAGAGTA TCAACAATGA GAGAACTCTG 
AAGACACAGG CTGTCAACAA ACTGGCTGAG ATAATGAATA GAAAAGATTT TAAAATAGAT 
AGAAAGAAAG CAAGCATGCA GGATTTGAGG AAGAAAGAGA AGGAAAACCG AAAGCTACAA 
TTGGAGCTTA ATCAAGAAAA AGAGAAATTC AATCAGATGG TAGTGAAATA CCAGAAGGAG 
CTTAATGAGA TGCAAGCGCA ACTAGCAGAA GAATCAACAT ACAGGAATGA GCTACAGATG 
CAGCTGGATA GTAAAGAGAG TGACATAGAA CAGCTACGAA GGAAAATTTT GGATCTCCAG 
CAAGGAATGG ATTCCACCAG TGTTTCGAGT CTACAGCCAG ATGAAATTGA TGGAAATCTT 
TCAGTTGTGC TTCTGATGTT AGTATTTCAT ACAGTTGATT CTTCCTGCAT TCCTTATTTA 
TTTATCTCCT ATGCTTATCT TCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133418.1
CDS	831..4277
Translation	MSTGESFESRFEKIDGTLKDPKSEVNVDCLLDGLDALVYDLDFPALRKNKNIDNFLNRYKDTVNKMRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSSRRVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKAGHLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNEISKEAKNLICAFLTDREVRLGRNGVEEIKRHLFFKDDQWAWETLRDTVAPVVPDLSSDIDTSNFDDIDEDKGEEETFPIPKAFVGNQLPFVGFTYYSNRRYLSVSAENSNDNRMGSSMDKSVMENMQKMIYDLEEQLHNEMQLKDEMEQKCRSSNITLDKIMKELDEEGNQRKNLELTVSQIEKEKMALQHKINDYQRKIEQESEKRRNVENEVSTLKDQMEDLKKISQHSQITNEKITQLQKQLEEANDLLRTESETAVRLRKGNTEMSKSLSQVESLNRELQERCRVLESAKLQVEKDYYQLQAALESERRDRSHGSEMIGELQVRITTLQEELKNLKNNLERVEAERKQAQDRLNHSEKEKNNLEIDLNYKLKSLQDRLEQEVNEHKVTKARLTDKHQSIEEAKSVAMCEMEKKVKEERAAREKAENRIVQAEKQCSMLDFDLKQSQQKVEHLLQQKERLEDEVKNLSLQLEQETNKRIMAQNELKAQAFEADNLKGSEKQLKQEINTLLEAKRLLEVELAQLAKQYRGNEGQMRELQDQLEAEQYFSTLYKTQVKELKEEIDEKNKETQRKMQELQNEKETLTTQLDLAETKAESEQLARALLEEQYFELSQESKKAASRHRQEMTDKDSIIRRLEETNSTLTKDVDLVTKENSEMSEKMKKQEEEYKLKKEEEINNIRMQYEKSINNERTLKTQAVNKLAEIMNRKDFKIDRKKASMQDLRKKEKENRKLQLELNQEKEKFNQMVVKYQKELNEMQAQLAEESTYRNELQMQLDSKESDIEQLRRKILDLQQGMDSTSVSSLQPDEIDGNLSVVLLMLVFHTVDSSCIPYLFISYAYLL

Target ORF information:

RefSeq Version	XM_015277932.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277932.1

ORF Insert Sequence:

ATGTCGACGG GAGAAAGTTT TGAGTCTCGG TTCGAAAAAA TCGACGGGAC TTTAAAGGAT 
CCCAAATCCG AAGTGAACGT GGACTGTTTG CTGGATGGGT TAGATGCTTT GGTATATGAC 
TTGGATTTTC CTGCCTTACG AAAAAACAAA AACATTGATA ACTTCTTAAA CAGGTATAAA 
GACACAGTAA ATAAAATGAG AGATCTGCGG ATGAAAGCTG AAGATTATGA AGTGGTGAAG 
GTGATTGGCA GAGGGGCATT TGGGGAGGTT CAGTTGGTAA GGCATAAATC ATCAAGAAGA 
GTGTATGCTA TGAAACTTCT GAGCAAATTT GAGATGATAA AAAGATCAGA TTCTGCGTTC 
TTCTGGGAAG AAAGGGATAT TATGGCTTTT GCTAATAGTC CCTGGGTTGT TCAGTTGTTT 
TACGCATTCC AAGATGATCG TTACCTTTAC ATGGTCATGG AATACATGCC TGGTGGAGAC 
CTTGTGAATT TAATGAGTAA CTATGATGTT CCTGAGAAAT GGGCAAGATT TTATACTGCT 
GAAGTTGTTC TTGCATTAGA TGCAATTCAC TCAATGGGCT TTATACACAG AGATGTGAAG 
CCTGACAATA TGCTGCTAGA TAAAGCTGGT CATTTAAAAC TAGCAGATTT TGGTACTTGT 
ATGAAAATGA ACAAGGAAGG TATGGTACGA TGTGATACAG CTGTAGGGAC ACCAGACTAT 
ATCTCTCCTG AAGTATTGAA ATCCCAGGGT GGTGATGGTT ACTATGGACG AGAATGTGAC 
TGGTGGTCAG TTGGAGTTTT TTTGTATGAG ATGCTTGTAG GTGACACGCC TTTTTATGCA 
GATTCTTTGG TTGGAACATA CAGTAAGATT ATGAACCACA AGAACTCCCT TACCTTCCCT 
GATGACAACG AGATCTCTAA AGAGGCAAAA AACCTTATTT GTGCCTTTTT AACTGATAGG 
GAAGTGAGAT TAGGACGAAA TGGCGTAGAA GAAATTAAAC GGCATCTTTT CTTCAAAGAT 
GATCAGTGGG CTTGGGAGAC TCTCAGAGAC ACTGTAGCAC CAGTTGTGCC CGACTTAAGT 
AGTGACATAG ACACTAGTAA TTTTGATGAT ATCGATGAAG ACAAAGGAGA GGAAGAAACA 
TTTCCCATCC CAAAAGCGTT TGTGGGCAAC CAGCTTCCTT TTGTTGGATT TACATATTAC 
AGCAACCGTC GGTATTTATC TGTCTCTGCA GAAAATTCCA ATGATAACAG AATGGGCTCC 
AGTATGGATA AAAGTGTGAT GGAAAATATG CAGAAGATGA TCTATGATTT GGAAGAACAG 
CTACATAATG AAATGCAGCT GAAAGATGAA ATGGAGCAAA AGTGCAGGTC TTCAAACATA 
ACGCTAGACA AGATAATGAA AGAACTGGAT GAAGAGGGAA ATCAAAGAAA GAACCTAGAA 
TTAACAGTGT CCCAGATTGA GAAGGAAAAG ATGGCACTGC AGCATAAGAT AAACGACTAC 
CAAAGGAAAA TTGAACAAGA GAGTGAAAAG AGACGGAATG TGGAAAATGA AGTGTCTACG 
CTAAAAGATC AAATGGAAGA CTTGAAAAAA ATCAGCCAAC ATTCACAAAT TACAAATGAG 
AAGATAACGC AGTTACAAAA ACAACTAGAA GAAGCCAATG ATTTGCTACG GACAGAATCA 
GAAACAGCAG TAAGACTGAG GAAGGGTAAC ACAGAAATGA GCAAGTCATT AAGTCAAGTA 
GAATCACTGA ATAGAGAACT GCAGGAAAGG TGTAGGGTTC TAGAAAGCGC AAAACTGCAG 
GTGGAAAAGG ATTATTATCA GCTGCAAGCA GCTTTGGAAT CAGAACGAAG GGACAGAAGT 
CATGGATCTG AAATGATTGG AGAACTACAA GTTCGGATTA CAACTTTACA AGAAGAATTA 
AAAAACCTTA AAAATAATCT TGAAAGAGTG GAAGCAGAAA GGAAACAAGC ACAGGATAGG 
CTTAATCATT CAGAAAAGGA AAAGAACAAT TTGGAGATAG ATTTGAACTA CAAACTCAAG 
TCTTTACAAG ACCGTTTAGA ACAGGAAGTA AATGAACACA AAGTGACTAA AGCTCGATTA 
ACTGACAAAC ATCAGTCAAT AGAGGAGGCA AAATCAGTTG CAATGTGCGA AATGGAAAAA 
AAAGTAAAGG AAGAAAGGGC AGCAAGAGAA AAGGCAGAGA ATCGAATAGT TCAGGCTGAA 
AAACAGTGTT CCATGTTAGA CTTTGACCTG AAGCAGTCTC AGCAGAAAGT GGAACACCTT 
CTTCAGCAAA AGGAAAGACT GGAGGATGAA GTAAAGAATC TGTCACTTCA GCTAGAGCAA 
GAGACAAATA AGCGAATAAT GGCACAGAAT GAATTAAAGG CACAAGCTTT TGAAGCAGAT 
AACTTGAAGG GTTCTGAGAA GCAACTGAAA CAAGAAATCA ATACGTTATT GGAAGCAAAA 
AGATTACTGG AAGTTGAGTT GGCTCAACTT GCTAAGCAGT ACAGAGGAAA TGAAGGTCAA 
ATGCGTGAGC TTCAGGATCA GCTTGAAGCG GAGCAGTATT TTTCGACACT TTACAAAACT 
CAGGTTAAGG AACTGAAGGA AGAAATTGAT GAAAAGAATA AAGAAACTCA AAGAAAAATG 
CAGGAATTGC AAAATGAGAA AGAAACACTC ACTACGCAGC TGGATTTAGC TGAAACCAAA 
GCTGAGTCGG AGCAGTTGGC ACGGGCACTT CTTGAAGAGC AGTATTTTGA GTTGAGTCAG 
GAGAGCAAAA AAGCAGCATC ACGACATAGG CAAGAAATGA CAGATAAGGA CAGCATCATA 
AGAAGGCTGG AAGAAACTAA CAGCACATTA ACCAAAGATG TTGATTTAGT AACAAAAGAA 
AATTCAGAGA TGAGTGAAAA AATGAAGAAA CAGGAGGAAG AATATAAGCT GAAAAAAGAG 
GAAGAAATAA ACAATATTAG AATGCAATAT GAGAAGAGTA TCAACAATGA GAGAACTCTG 
AAGACACAGG CTGTCAACAA ACTGGCTGAG ATAATGAATA GAAAAGATTT TAAAATAGAT 
AGAAAGAAAG CAAGCATGCA GGATTTGAGG AAGAAAGAGA AGGAAAACCG AAAGCTACAA 
TTGGAGCTTA ATCAAGAAAA AGAGAAATTC AATCAGATGG TAGTGAAATA CCAGAAGGAG 
CTTAATGAGA TGCAAGCGCA ACTAGCAGAA GAATCAACAT ACAGGAATGA GCTACAGATG 
CAGCTGGATA GTAAAGAGAG TGACATAGAA CAGCTACGAA GGAAAATTTT GGATCTCCAG 
CAAGGAATGG ATTCCACCAG TGTTTCGAGT CTACAGCCAG ATGAAATTGA TGGAAATCTT 
TCAGTTGTGC TTCTGATGTT AGTATTTCAT ACAGTTGATT CTTCCTGCAT TCCTTATTTA 
TTTATCTCCT ATGCTTATCT TCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK1 ( XM_015277932.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133418.1 Gallus gallus Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), transcript variant X2, mRNA.

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CloneID	OGa32476
Clone ID Related Accession (Same CDS sequence)	XM_015277932.1 , XM_015277932.2
Accession Version	XM_015277932.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	rho-associated protein kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277932.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACGG GAGAAAGTTT TGAGTCTCGG TTCGAAAAAA TCGACGGGAC TTTAAAGGAT 
CCCAAATCCG AAGTGAACGT GGACTGTTTG CTGGATGGGT TAGATGCTTT GGTATATGAC 
TTGGATTTTC CTGCCTTACG AAAAAACAAA AACATTGATA ACTTCTTAAA CAGGTATAAA 
GACACAGTAA ATAAAATGAG AGATCTGCGG ATGAAAGCTG AAGATTATGA AGTGGTGAAG 
GTGATTGGCA GAGGGGCATT TGGGGAGGTT CAGTTGGTAA GGCATAAATC ATCAAGAAGA 
GTGTATGCTA TGAAACTTCT GAGCAAATTT GAGATGATAA AAAGATCAGA TTCTGCGTTC 
TTCTGGGAAG AAAGGGATAT TATGGCTTTT GCTAATAGTC CCTGGGTTGT TCAGTTGTTT 
TACGCATTCC AAGATGATCG TTACCTTTAC ATGGTCATGG AATACATGCC TGGTGGAGAC 
CTTGTGAATT TAATGAGTAA CTATGATGTT CCTGAGAAAT GGGCAAGATT TTATACTGCT 
GAAGTTGTTC TTGCATTAGA TGCAATTCAC TCAATGGGCT TTATACACAG AGATGTGAAG 
CCTGACAATA TGCTGCTAGA TAAAGCTGGT CATTTAAAAC TAGCAGATTT TGGTACTTGT 
ATGAAAATGA ACAAGGAAGG TATGGTACGA TGTGATACAG CTGTAGGGAC ACCAGACTAT 
ATCTCTCCTG AAGTATTGAA ATCCCAGGGT GGTGATGGTT ACTATGGACG AGAATGTGAC 
TGGTGGTCAG TTGGAGTTTT TTTGTATGAG ATGCTTGTAG GTGACACGCC TTTTTATGCA 
GATTCTTTGG TTGGAACATA CAGTAAGATT ATGAACCACA AGAACTCCCT TACCTTCCCT 
GATGACAACG AGATCTCTAA AGAGGCAAAA AACCTTATTT GTGCCTTTTT AACTGATAGG 
GAAGTGAGAT TAGGACGAAA TGGCGTAGAA GAAATTAAAC GGCATCTTTT CTTCAAAGAT 
GATCAGTGGG CTTGGGAGAC TCTCAGAGAC ACTGTAGCAC CAGTTGTGCC CGACTTAAGT 
AGTGACATAG ACACTAGTAA TTTTGATGAT ATCGATGAAG ACAAAGGAGA GGAAGAAACA 
TTTCCCATCC CAAAAGCGTT TGTGGGCAAC CAGCTTCCTT TTGTTGGATT TACATATTAC 
AGCAACCGTC GGTATTTATC TGTCTCTGCA GAAAATTCCA ATGATAACAG AATGGGCTCC 
AGTATGGATA AAAGTGTGAT GGAAAATATG CAGAAGATGA TCTATGATTT GGAAGAACAG 
CTACATAATG AAATGCAGCT GAAAGATGAA ATGGAGCAAA AGTGCAGGTC TTCAAACATA 
ACGCTAGACA AGATAATGAA AGAACTGGAT GAAGAGGGAA ATCAAAGAAA GAACCTAGAA 
TTAACAGTGT CCCAGATTGA GAAGGAAAAG ATGGCACTGC AGCATAAGAT AAACGACTAC 
CAAAGGAAAA TTGAACAAGA GAGTGAAAAG AGACGGAATG TGGAAAATGA AGTGTCTACG 
CTAAAAGATC AAATGGAAGA CTTGAAAAAA ATCAGCCAAC ATTCACAAAT TACAAATGAG 
AAGATAACGC AGTTACAAAA ACAACTAGAA GAAGCCAATG ATTTGCTACG GACAGAATCA 
GAAACAGCAG TAAGACTGAG GAAGGGTAAC ACAGAAATGA GCAAGTCATT AAGTCAAGTA 
GAATCACTGA ATAGAGAACT GCAGGAAAGG TGTAGGGTTC TAGAAAGCGC AAAACTGCAG 
GTGGAAAAGG ATTATTATCA GCTGCAAGCA GCTTTGGAAT CAGAACGAAG GGACAGAAGT 
CATGGATCTG AAATGATTGG AGAACTACAA GTTCGGATTA CAACTTTACA AGAAGAATTA 
AAAAACCTTA AAAATAATCT TGAAAGAGTG GAAGCAGAAA GGAAACAAGC ACAGGATAGG 
CTTAATCATT CAGAAAAGGA AAAGAACAAT TTGGAGATAG ATTTGAACTA CAAACTCAAG 
TCTTTACAAG ACCGTTTAGA ACAGGAAGTA AATGAACACA AAGTGACTAA AGCTCGATTA 
ACTGACAAAC ATCAGTCAAT AGAGGAGGCA AAATCAGTTG CAATGTGCGA AATGGAAAAA 
AAAGTAAAGG AAGAAAGGGC AGCAAGAGAA AAGGCAGAGA ATCGAATAGT TCAGGCTGAA 
AAACAGTGTT CCATGTTAGA CTTTGACCTG AAGCAGTCTC AGCAGAAAGT GGAACACCTT 
CTTCAGCAAA AGGAAAGACT GGAGGATGAA GTAAAGAATC TGTCACTTCA GCTAGAGCAA 
GAGACAAATA AGCGAATAAT GGCACAGAAT GAATTAAAGG CACAAGCTTT TGAAGCAGAT 
AACTTGAAGG GTTCTGAGAA GCAACTGAAA CAAGAAATCA ATACGTTATT GGAAGCAAAA 
AGATTACTGG AAGTTGAGTT GGCTCAACTT GCTAAGCAGT ACAGAGGAAA TGAAGGTCAA 
ATGCGTGAGC TTCAGGATCA GCTTGAAGCG GAGCAGTATT TTTCGACACT TTACAAAACT 
CAGGTTAAGG AACTGAAGGA AGAAATTGAT GAAAAGAATA AAGAAACTCA AAGAAAAATG 
CAGGAATTGC AAAATGAGAA AGAAACACTC ACTACGCAGC TGGATTTAGC TGAAACCAAA 
GCTGAGTCGG AGCAGTTGGC ACGGGCACTT CTTGAAGAGC AGTATTTTGA GTTGAGTCAG 
GAGAGCAAAA AAGCAGCATC ACGACATAGG CAAGAAATGA CAGATAAGGA CAGCATCATA 
AGAAGGCTGG AAGAAACTAA CAGCACATTA ACCAAAGATG TTGATTTAGT AACAAAAGAA 
AATTCAGAGA TGAGTGAAAA AATGAAGAAA CAGGAGGAAG AATATAAGCT GAAAAAAGAG 
GAAGAAATAA ACAATATTAG AATGCAATAT GAGAAGAGTA TCAACAATGA GAGAACTCTG 
AAGACACAGG CTGTCAACAA ACTGGCTGAG ATAATGAATA GAAAAGATTT TAAAATAGAT 
AGAAAGAAAG CAAGCATGCA GGATTTGAGG AAGAAAGAGA AGGAAAACCG AAAGCTACAA 
TTGGAGCTTA ATCAAGAAAA AGAGAAATTC AATCAGATGG TAGTGAAATA CCAGAAGGAG 
CTTAATGAGA TGCAAGCGCA ACTAGCAGAA GAATCAACAT ACAGGAATGA GCTACAGATG 
CAGCTGGATA GTAAAGAGAG TGACATAGAA CAGCTACGAA GGAAAATTTT GGATCTCCAG 
CAAGGAATGG ATTCCACCAG TGTTTCGAGT CTACAGCCAG ATGAAATTGA TGGAAATCTT 
TCAGTTGTGC TTCTGATGTT AGTATTTCAT ACAGTTGATT CTTCCTGCAT TCCTTATTTA 
TTTATCTCCT ATGCTTATCT TCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133418.1
CDS	88..3534
Translation	MSTGESFESRFEKIDGTLKDPKSEVNVDCLLDGLDALVYDLDFPALRKNKNIDNFLNRYKDTVNKMRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSSRRVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKAGHLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNEISKEAKNLICAFLTDREVRLGRNGVEEIKRHLFFKDDQWAWETLRDTVAPVVPDLSSDIDTSNFDDIDEDKGEEETFPIPKAFVGNQLPFVGFTYYSNRRYLSVSAENSNDNRMGSSMDKSVMENMQKMIYDLEEQLHNEMQLKDEMEQKCRSSNITLDKIMKELDEEGNQRKNLELTVSQIEKEKMALQHKINDYQRKIEQESEKRRNVENEVSTLKDQMEDLKKISQHSQITNEKITQLQKQLEEANDLLRTESETAVRLRKGNTEMSKSLSQVESLNRELQERCRVLESAKLQVEKDYYQLQAALESERRDRSHGSEMIGELQVRITTLQEELKNLKNNLERVEAERKQAQDRLNHSEKEKNNLEIDLNYKLKSLQDRLEQEVNEHKVTKARLTDKHQSIEEAKSVAMCEMEKKVKEERAAREKAENRIVQAEKQCSMLDFDLKQSQQKVEHLLQQKERLEDEVKNLSLQLEQETNKRIMAQNELKAQAFEADNLKGSEKQLKQEINTLLEAKRLLEVELAQLAKQYRGNEGQMRELQDQLEAEQYFSTLYKTQVKELKEEIDEKNKETQRKMQELQNEKETLTTQLDLAETKAESEQLARALLEEQYFELSQESKKAASRHRQEMTDKDSIIRRLEETNSTLTKDVDLVTKENSEMSEKMKKQEEEYKLKKEEEINNIRMQYEKSINNERTLKTQAVNKLAEIMNRKDFKIDRKKASMQDLRKKEKENRKLQLELNQEKEKFNQMVVKYQKELNEMQAQLAEESTYRNELQMQLDSKESDIEQLRRKILDLQQGMDSTSVSSLQPDEIDGNLSVVLLMLVFHTVDSSCIPYLFISYAYLL

Target ORF information:

RefSeq Version	XM_015277932.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277932.2

ORF Insert Sequence:

ATGTCGACGG GAGAAAGTTT TGAGTCTCGG TTCGAAAAAA TCGACGGGAC TTTAAAGGAT 
CCCAAATCCG AAGTGAACGT GGACTGTTTG CTGGATGGGT TAGATGCTTT GGTATATGAC 
TTGGATTTTC CTGCCTTACG AAAAAACAAA AACATTGATA ACTTCTTAAA CAGGTATAAA 
GACACAGTAA ATAAAATGAG AGATCTGCGG ATGAAAGCTG AAGATTATGA AGTGGTGAAG 
GTGATTGGCA GAGGGGCATT TGGGGAGGTT CAGTTGGTAA GGCATAAATC ATCAAGAAGA 
GTGTATGCTA TGAAACTTCT GAGCAAATTT GAGATGATAA AAAGATCAGA TTCTGCGTTC 
TTCTGGGAAG AAAGGGATAT TATGGCTTTT GCTAATAGTC CCTGGGTTGT TCAGTTGTTT 
TACGCATTCC AAGATGATCG TTACCTTTAC ATGGTCATGG AATACATGCC TGGTGGAGAC 
CTTGTGAATT TAATGAGTAA CTATGATGTT CCTGAGAAAT GGGCAAGATT TTATACTGCT 
GAAGTTGTTC TTGCATTAGA TGCAATTCAC TCAATGGGCT TTATACACAG AGATGTGAAG 
CCTGACAATA TGCTGCTAGA TAAAGCTGGT CATTTAAAAC TAGCAGATTT TGGTACTTGT 
ATGAAAATGA ACAAGGAAGG TATGGTACGA TGTGATACAG CTGTAGGGAC ACCAGACTAT 
ATCTCTCCTG AAGTATTGAA ATCCCAGGGT GGTGATGGTT ACTATGGACG AGAATGTGAC 
TGGTGGTCAG TTGGAGTTTT TTTGTATGAG ATGCTTGTAG GTGACACGCC TTTTTATGCA 
GATTCTTTGG TTGGAACATA CAGTAAGATT ATGAACCACA AGAACTCCCT TACCTTCCCT 
GATGACAACG AGATCTCTAA AGAGGCAAAA AACCTTATTT GTGCCTTTTT AACTGATAGG 
GAAGTGAGAT TAGGACGAAA TGGCGTAGAA GAAATTAAAC GGCATCTTTT CTTCAAAGAT 
GATCAGTGGG CTTGGGAGAC TCTCAGAGAC ACTGTAGCAC CAGTTGTGCC CGACTTAAGT 
AGTGACATAG ACACTAGTAA TTTTGATGAT ATCGATGAAG ACAAAGGAGA GGAAGAAACA 
TTTCCCATCC CAAAAGCGTT TGTGGGCAAC CAGCTTCCTT TTGTTGGATT TACATATTAC 
AGCAACCGTC GGTATTTATC TGTCTCTGCA GAAAATTCCA ATGATAACAG AATGGGCTCC 
AGTATGGATA AAAGTGTGAT GGAAAATATG CAGAAGATGA TCTATGATTT GGAAGAACAG 
CTACATAATG AAATGCAGCT GAAAGATGAA ATGGAGCAAA AGTGCAGGTC TTCAAACATA 
ACGCTAGACA AGATAATGAA AGAACTGGAT GAAGAGGGAA ATCAAAGAAA GAACCTAGAA 
TTAACAGTGT CCCAGATTGA GAAGGAAAAG ATGGCACTGC AGCATAAGAT AAACGACTAC 
CAAAGGAAAA TTGAACAAGA GAGTGAAAAG AGACGGAATG TGGAAAATGA AGTGTCTACG 
CTAAAAGATC AAATGGAAGA CTTGAAAAAA ATCAGCCAAC ATTCACAAAT TACAAATGAG 
AAGATAACGC AGTTACAAAA ACAACTAGAA GAAGCCAATG ATTTGCTACG GACAGAATCA 
GAAACAGCAG TAAGACTGAG GAAGGGTAAC ACAGAAATGA GCAAGTCATT AAGTCAAGTA 
GAATCACTGA ATAGAGAACT GCAGGAAAGG TGTAGGGTTC TAGAAAGCGC AAAACTGCAG 
GTGGAAAAGG ATTATTATCA GCTGCAAGCA GCTTTGGAAT CAGAACGAAG GGACAGAAGT 
CATGGATCTG AAATGATTGG AGAACTACAA GTTCGGATTA CAACTTTACA AGAAGAATTA 
AAAAACCTTA AAAATAATCT TGAAAGAGTG GAAGCAGAAA GGAAACAAGC ACAGGATAGG 
CTTAATCATT CAGAAAAGGA AAAGAACAAT TTGGAGATAG ATTTGAACTA CAAACTCAAG 
TCTTTACAAG ACCGTTTAGA ACAGGAAGTA AATGAACACA AAGTGACTAA AGCTCGATTA 
ACTGACAAAC ATCAGTCAAT AGAGGAGGCA AAATCAGTTG CAATGTGCGA AATGGAAAAA 
AAAGTAAAGG AAGAAAGGGC AGCAAGAGAA AAGGCAGAGA ATCGAATAGT TCAGGCTGAA 
AAACAGTGTT CCATGTTAGA CTTTGACCTG AAGCAGTCTC AGCAGAAAGT GGAACACCTT 
CTTCAGCAAA AGGAAAGACT GGAGGATGAA GTAAAGAATC TGTCACTTCA GCTAGAGCAA 
GAGACAAATA AGCGAATAAT GGCACAGAAT GAATTAAAGG CACAAGCTTT TGAAGCAGAT 
AACTTGAAGG GTTCTGAGAA GCAACTGAAA CAAGAAATCA ATACGTTATT GGAAGCAAAA 
AGATTACTGG AAGTTGAGTT GGCTCAACTT GCTAAGCAGT ACAGAGGAAA TGAAGGTCAA 
ATGCGTGAGC TTCAGGATCA GCTTGAAGCG GAGCAGTATT TTTCGACACT TTACAAAACT 
CAGGTTAAGG AACTGAAGGA AGAAATTGAT GAAAAGAATA AAGAAACTCA AAGAAAAATG 
CAGGAATTGC AAAATGAGAA AGAAACACTC ACTACGCAGC TGGATTTAGC TGAAACCAAA 
GCTGAGTCGG AGCAGTTGGC ACGGGCACTT CTTGAAGAGC AGTATTTTGA GTTGAGTCAG 
GAGAGCAAAA AAGCAGCATC ACGACATAGG CAAGAAATGA CAGATAAGGA CAGCATCATA 
AGAAGGCTGG AAGAAACTAA CAGCACATTA ACCAAAGATG TTGATTTAGT AACAAAAGAA 
AATTCAGAGA TGAGTGAAAA AATGAAGAAA CAGGAGGAAG AATATAAGCT GAAAAAAGAG 
GAAGAAATAA ACAATATTAG AATGCAATAT GAGAAGAGTA TCAACAATGA GAGAACTCTG 
AAGACACAGG CTGTCAACAA ACTGGCTGAG ATAATGAATA GAAAAGATTT TAAAATAGAT 
AGAAAGAAAG CAAGCATGCA GGATTTGAGG AAGAAAGAGA AGGAAAACCG AAAGCTACAA 
TTGGAGCTTA ATCAAGAAAA AGAGAAATTC AATCAGATGG TAGTGAAATA CCAGAAGGAG 
CTTAATGAGA TGCAAGCGCA ACTAGCAGAA GAATCAACAT ACAGGAATGA GCTACAGATG 
CAGCTGGATA GTAAAGAGAG TGACATAGAA CAGCTACGAA GGAAAATTTT GGATCTCCAG 
CAAGGAATGG ATTCCACCAG TGTTTCGAGT CTACAGCCAG ATGAAATTGA TGGAAATCTT 
TCAGTTGTGC TTCTGATGTT AGTATTTCAT ACAGTTGATT CTTCCTGCAT TCCTTATTTA 
TTTATCTCCT ATGCTTATCT TCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK1 ( NM_001199448.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001186377.1 Gallus gallus Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), mRNA.

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CloneID	OGa00285
Clone ID Related Accession (Same CDS sequence)	NM_001199448.1
Accession Version	NM_001199448.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-25
Organism	Gallus gallus(chicken)
Product	rho-associated protein kinase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AADN05000027.1. On Dec 7, 2010 this sequence version replaced XM_419151.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

ATGTCGACGG GAGAAAGTTT TGAGTCTCGG TTCGAAAAAA TCGACGGGAC TTTAAAGGAT 
CCCAAATCCG AAGTGAACGT GGACTGTTTG CTGGATGGGT TAGATGCTTT GGTATATGAC 
TTGGATTTTC CTGCCTTACG AAAAAACAAA AACATTGATA ACTTCTTAAA CAGGTATAAA 
GACACAGTAA ATAAAATGAG AGATCTGCGG ATGAAAGCTG AAGATTATGA AGTGGTGAAG 
GTGATTGGCA GAGGGGCATT TGGGGAGGTT CAGTTGGTAA GGCATAAATC ATCAAGAAGA 
GTGTATGCTA TGAAACTTCT GAGCAAATTT GAGATGATAA AAAGATCAGA TTCTGCGTTC 
TTCTGGGAAG AAAGGGATAT TATGGCTTTT GCTAATAGTC CCTGGGTTGT TCAGTTGTTT 
TACGCATTCC AAGATGATCG TTACCTTTAC ATGGTCATGG AATACATGCC TGGTGGAGAC 
CTTGTGAATT TAATGAGTAA CTATGATGTT CCTGAGAAAT GGGCAAGATT TTATACTGCT 
GAAGTTGTTC TTGCATTAGA TGCAATTCAC TCAATGGGCT TTATACACAG AGATGTGAAG 
CCTGACAATA TGCTGCTAGA TAAAGCTGGT CATTTAAAAC TAGCAGATTT TGGTACTTGT 
ATGAAAATGA ACAAGGAAGG TATGGTACGA TGTGATACAG CTGTAGGGAC ACCAGACTAT 
ATCTCTCCTG AAGTATTGAA ATCCCAGGGT GGTGATGGTT ACTATGGACG AGAATGTGAC 
TGGTGGTCAG TTGGAGTTTT TTTGTATGAG ATGCTTGTAG GTGACACGCC TTTTTATGCA 
GATTCTTTGG TTGGAACATA CAGTAAGATT ATGAACCACA AGAACTCCCT TACCTTCCCT 
GATGACAACG AGATCTCTAA AGAGGCAAAA AACCTTATTT GTGCCTTTTT AACTGATAGG 
GAAGTGAGAT TAGGACGAAA TGGCGTAGAA GAAATTAAAC GGCATCTTTT CTTCAAAGAT 
GATCAGTGGG CTTGGGAGAC TCTCAGAGAC ACTGTAGCAC CAGTTGTGCC CGACTTAAGT 
AGTGACATAG ACACTAGTAA TTTTGATGAT ATCGATGAAG ACAAAGGAGA GGAAGAAACA 
TTTCCCATCC CAAAAGCGTT TGTGGGCAAC CAGCTTCCTT TTGTTGGATT TACATATTAC 
AGCAACCGTC GGTATTTATC TGTCTCTGCA GAAAATTCCA ATGATAACAG AATGGGCTCC 
AGTATGGATA AAAGTGTGAT GGAAAATATG CAGAAGATGA TCTATGATTT GGAAGAACAG 
CTACATAATG AAATGCAGCT GAAAGATGAA ATGGAGCAAA AGTGCAGGTC TTCAAACATA 
ACGCTAGACA AGATAATGAA AGAACTGGAT GAAGAGGGAA ATCAAAGAAA GAACCTAGAA 
TTAACAGTGT CCCAGATTGA GAAGGAAAAG ATGGCACTGC AGCATAAGAT AAACGACTAC 
CAAAGGAAAA TTGAACAAGA GAGTGAAAAG AGACGGAATG TGGAAAATGA AGTGTCTACG 
CTAAAAGATC AAATGGAAGA CTTGAAAAAA ATCAGCCAAC ATTCACAAAT TACAAATGAG 
AAGATAACGC AGTTACAAAA ACAACTAGAA GAAGCCAATG ATTTGCTACG GACAGAATCA 
GAAACAGCAG TAAGACTGAG GAAGGGTAAC ACAGAAATGA GCAAGTCATT AAGTCAAGTA 
GAATCACTGA ATAGAGAACT GCAGGAAAGG TGTAGGGTTC TAGAAAGCGC AAAACTGCAG 
GTGGAAAAGG ATTATTATCA GCTGCAAGCA GCTTTGGAAT CAGAACGAAG GGACAGAAGT 
CATGGATCTG AAATGATTGG AGAACTACAA GTTCGGATTA CAACTTTACA AGAAGAATTA 
AAAAACCTTA AAAATAATCT TGAAAGAGTG GAAGCAGAAA GGAAACAAGC ACAGGATAGG 
CTTAATCATT CAGAAAAGGA AAAGAACAAT TTGGAGATAG ATTTGAACTA CAAACTCAAG 
TCTTTACAAG ACCGTTTAGA ACAGGAAGTA AATGAACACA AAGTGACTAA AGCTCGATTA 
ACTGACAAAC ATCAGTCAAT AGAGGAGGCA AAATCAGTTG CAATGTGCGA AATGGAAAAA 
AAAGTAAAGG AAGAAAGGGC AGCAAGAGAA AAGGCAGAGA ATCGAATAGT TCAGGCTGAA 
AAACAGTGTT CCATGTTAGA CTTTGACCTG AAGCAGTCTC AGCAGAAAGT GGAACACCTT 
CTTCAGCAAA AGGAAAGACT GGAGGATGAA GTAAAGAATC TGTCACTTCA GCTAGAGCAA 
GAGACAAATA AGCGAATAAT GGCACAGAAT GAATTAAAGG CACAAGCTTT TGAAGCAGAT 
AACTTGAAGG GTTCTGAGAA GCAACTGAAA CAAGAAATCA ATACGTTATT GGAAGCAAAA 
AGATTACTGG AAGTTGAGTT GGCTCAACTT GCTAAGCAGT ACAGAGGAAA TGAAGGTCAA 
ATGCGTGAGC TTCAGGATCA GCTTGAAGCG GAGCAGTATT TTTCGACACT TTACAAAACT 
CAGGTTAAGG AACTGAAGGA AGAAATTGAT GAAAAGAATA AAGAAACTCA AAGAAAAATG 
CAGGAATTGC AAAATGAGAA AGAAACACTC ACTACGCAGC TGGATTTAGC TGAAACCAAA 
GCTGAGTCGG AGCAGTTGGC ACGGGCACTT CTTGAAGAGC AGTATTTTGA GTTGAGTCAG 
GAGAGCAAAA AAGCAGCATC ACGACATAGG CAAGAAATGA CAGATAAGGA CAGCATCATA 
AGAAGGCTGG AAGAAACTAA CAGCACATTA ACCAAAGATG TTGATTTAGT AACAAAAGAA 
AATTCAGAGA TGAGTGAAAA AATGAAGAAA CAGGAGGAAG AATATAAGCT GAAAAAAGAG 
GAAGAAATAA ACAATATTAG AATGCAATAT GAGAAGAGTA TCAACAATGA GAGAACTCTG 
AAGACACAGG CTGTCAACAA ACTGGCTGAG ATAATGAATA GAAAAGATTT TAAAATAGAT 
AGAAAGAAAG CAAGCATGCA GGATTTGAGG AAGAAAGAGA AGGAAAACCG AAAGCTACAA 
TTGGAGCTTA ATCAAGAAAA AGAGAAATTC AATCAGATGG TAGTGAAATA CCAGAAGGAG 
CTTAATGAGA TGCAAGCGCA ACTAGCAGAA GAATCAACAT ACAGGAATGA GCTACAGATG 
CAGCTGGATA GTAAAGAGAG TGACATAGAA CAGCTACGAA GGAAAATTTT GGATCTCCAG 
CAAGGAATGG ATTCCACCAG TGTTTCGAGT CTACAGCCAG ATGAAATTGA TGGAAATCTT 
TCAGAATCAA GGCTGGAAGG TTGGCTTTCA ATACCAAACA AGGGAAACAT AAAGCGACAC 
GGCTGGAAGA AACAGTACGT TGTGGTGAGC AGTAAAAAGA TACTGTTCTA TAATGATGAA 
AAGGACAAGG ACCAATCTAA TCCATCCATG GTACTGGACA TAGATAAGCT GTTCCACGTC 
CGACCTGTAA CTCAAGGAGA TGTATATAGA GCAGAAACTG AAGAAATTCC TAAAATATTC 
CAGATTCTGT ATGCTAATGA GGGGGAATGC AGGAAGGATG TGGAGGTAGA ATCAGTCCAG 
CCTGCAGAGA AGACAAACTT TCTAAATCAT AAAGGCCATG AATTTATTCC AACAATATAC 
CACTTTCCAG CCAACTGTGA GGCCTGTGCC AAACCTCTTT GGCATGTCTT TAAACCCCCA 
GCTGCCCTGG AATGTCGAAG ATGCCATGTG AAGTGCCATA GAGACCACTT GGATAAAAAG 
GAGGAGTTAA TTGCACCGTG CAAAGTGAGT TACGACGTAA CTTCAGCGAG AGATATGTTG 
TTGCTGGCAT CCTGTCAAGA TGAACAGAAG AAATGGGTGA CTCATTTAGT AAAGAAGATC 
CCGAAGAACC CACCGTCTAC TTTTGTTCGT GCTTCTCCCA GAACTATGTC TACAAGATCC 
TCAGCAAACC AGTCATTCCG AAAAGCTGTT AAAAATACTT CTGGAAAAAC GAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186377.1
CDS	1..4077
Translation	MSTGESFESRFEKIDGTLKDPKSEVNVDCLLDGLDALVYDLDFPALRKNKNIDNFLNRYKDTVNKMRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSSRRVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKAGHLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNEISKEAKNLICAFLTDREVRLGRNGVEEIKRHLFFKDDQWAWETLRDTVAPVVPDLSSDIDTSNFDDIDEDKGEEETFPIPKAFVGNQLPFVGFTYYSNRRYLSVSAENSNDNRMGSSMDKSVMENMQKMIYDLEEQLHNEMQLKDEMEQKCRSSNITLDKIMKELDEEGNQRKNLELTVSQIEKEKMALQHKINDYQRKIEQESEKRRNVENEVSTLKDQMEDLKKISQHSQITNEKITQLQKQLEEANDLLRTESETAVRLRKGNTEMSKSLSQVESLNRELQERCRVLESAKLQVEKDYYQLQAALESERRDRSHGSEMIGELQVRITTLQEELKNLKNNLERVEAERKQAQDRLNHSEKEKNNLEIDLNYKLKSLQDRLEQEVNEHKVTKARLTDKHQSIEEAKSVAMCEMEKKVKEERAAREKAENRIVQAEKQCSMLDFDLKQSQQKVEHLLQQKERLEDEVKNLSLQLEQETNKRIMAQNELKAQAFEADNLKGSEKQLKQEINTLLEAKRLLEVELAQLAKQYRGNEGQMRELQDQLEAEQYFSTLYKTQVKELKEEIDEKNKETQRKMQELQNEKETLTTQLDLAETKAESEQLARALLEEQYFELSQESKKAASRHRQEMTDKDSIIRRLEETNSTLTKDVDLVTKENSEMSEKMKKQEEEYKLKKEEEINNIRMQYEKSINNERTLKTQAVNKLAEIMNRKDFKIDRKKASMQDLRKKEKENRKLQLELNQEKEKFNQMVVKYQKELNEMQAQLAEESTYRNELQMQLDSKESDIEQLRRKILDLQQGMDSTSVSSLQPDEIDGNLSESRLEGWLSIPNKGNIKRHGWKKQYVVVSSKKILFYNDEKDKDQSNPSMVLDIDKLFHVRPVTQGDVYRAETEEIPKIFQILYANEGECRKDVEVESVQPAEKTNFLNHKGHEFIPTIYHFPANCEACAKPLWHVFKPPAALECRRCHVKCHRDHLDKKEELIAPCKVSYDVTSARDMLLLASCQDEQKKWVTHLVKKIPKNPPSTFVRASPRTMSTRSSANQSFRKAVKNTSGKTS

Target ORF information:

RefSeq Version	NM_001199448.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199448.1

ORF Insert Sequence:

ATGTCGACGG GAGAAAGTTT TGAGTCTCGG TTCGAAAAAA TCGACGGGAC TTTAAAGGAT 
CCCAAATCCG AAGTGAACGT GGACTGTTTG CTGGATGGGT TAGATGCTTT GGTATATGAC 
TTGGATTTTC CTGCCTTACG AAAAAACAAA AACATTGATA ACTTCTTAAA CAGGTATAAA 
GACACAGTAA ATAAAATGAG AGATCTGCGG ATGAAAGCTG AAGATTATGA AGTGGTGAAG 
GTGATTGGCA GAGGGGCATT TGGGGAGGTT CAGTTGGTAA GGCATAAATC ATCAAGAAGA 
GTGTATGCTA TGAAACTTCT GAGCAAATTT GAGATGATAA AAAGATCAGA TTCTGCGTTC 
TTCTGGGAAG AAAGGGATAT TATGGCTTTT GCTAATAGTC CCTGGGTTGT TCAGTTGTTT 
TACGCATTCC AAGATGATCG TTACCTTTAC ATGGTCATGG AATACATGCC TGGTGGAGAC 
CTTGTGAATT TAATGAGTAA CTATGATGTT CCTGAGAAAT GGGCAAGATT TTATACTGCT 
GAAGTTGTTC TTGCATTAGA TGCAATTCAC TCAATGGGCT TTATACACAG AGATGTGAAG 
CCTGACAATA TGCTGCTAGA TAAAGCTGGT CATTTAAAAC TAGCAGATTT TGGTACTTGT 
ATGAAAATGA ACAAGGAAGG TATGGTACGA TGTGATACAG CTGTAGGGAC ACCAGACTAT 
ATCTCTCCTG AAGTATTGAA ATCCCAGGGT GGTGATGGTT ACTATGGACG AGAATGTGAC 
TGGTGGTCAG TTGGAGTTTT TTTGTATGAG ATGCTTGTAG GTGACACGCC TTTTTATGCA 
GATTCTTTGG TTGGAACATA CAGTAAGATT ATGAACCACA AGAACTCCCT TACCTTCCCT 
GATGACAACG AGATCTCTAA AGAGGCAAAA AACCTTATTT GTGCCTTTTT AACTGATAGG 
GAAGTGAGAT TAGGACGAAA TGGCGTAGAA GAAATTAAAC GGCATCTTTT CTTCAAAGAT 
GATCAGTGGG CTTGGGAGAC TCTCAGAGAC ACTGTAGCAC CAGTTGTGCC CGACTTAAGT 
AGTGACATAG ACACTAGTAA TTTTGATGAT ATCGATGAAG ACAAAGGAGA GGAAGAAACA 
TTTCCCATCC CAAAAGCGTT TGTGGGCAAC CAGCTTCCTT TTGTTGGATT TACATATTAC 
AGCAACCGTC GGTATTTATC TGTCTCTGCA GAAAATTCCA ATGATAACAG AATGGGCTCC 
AGTATGGATA AAAGTGTGAT GGAAAATATG CAGAAGATGA TCTATGATTT GGAAGAACAG 
CTACATAATG AAATGCAGCT GAAAGATGAA ATGGAGCAAA AGTGCAGGTC TTCAAACATA 
ACGCTAGACA AGATAATGAA AGAACTGGAT GAAGAGGGAA ATCAAAGAAA GAACCTAGAA 
TTAACAGTGT CCCAGATTGA GAAGGAAAAG ATGGCACTGC AGCATAAGAT AAACGACTAC 
CAAAGGAAAA TTGAACAAGA GAGTGAAAAG AGACGGAATG TGGAAAATGA AGTGTCTACG 
CTAAAAGATC AAATGGAAGA CTTGAAAAAA ATCAGCCAAC ATTCACAAAT TACAAATGAG 
AAGATAACGC AGTTACAAAA ACAACTAGAA GAAGCCAATG ATTTGCTACG GACAGAATCA 
GAAACAGCAG TAAGACTGAG GAAGGGTAAC ACAGAAATGA GCAAGTCATT AAGTCAAGTA 
GAATCACTGA ATAGAGAACT GCAGGAAAGG TGTAGGGTTC TAGAAAGCGC AAAACTGCAG 
GTGGAAAAGG ATTATTATCA GCTGCAAGCA GCTTTGGAAT CAGAACGAAG GGACAGAAGT 
CATGGATCTG AAATGATTGG AGAACTACAA GTTCGGATTA CAACTTTACA AGAAGAATTA 
AAAAACCTTA AAAATAATCT TGAAAGAGTG GAAGCAGAAA GGAAACAAGC ACAGGATAGG 
CTTAATCATT CAGAAAAGGA AAAGAACAAT TTGGAGATAG ATTTGAACTA CAAACTCAAG 
TCTTTACAAG ACCGTTTAGA ACAGGAAGTA AATGAACACA AAGTGACTAA AGCTCGATTA 
ACTGACAAAC ATCAGTCAAT AGAGGAGGCA AAATCAGTTG CAATGTGCGA AATGGAAAAA 
AAAGTAAAGG AAGAAAGGGC AGCAAGAGAA AAGGCAGAGA ATCGAATAGT TCAGGCTGAA 
AAACAGTGTT CCATGTTAGA CTTTGACCTG AAGCAGTCTC AGCAGAAAGT GGAACACCTT 
CTTCAGCAAA AGGAAAGACT GGAGGATGAA GTAAAGAATC TGTCACTTCA GCTAGAGCAA 
GAGACAAATA AGCGAATAAT GGCACAGAAT GAATTAAAGG CACAAGCTTT TGAAGCAGAT 
AACTTGAAGG GTTCTGAGAA GCAACTGAAA CAAGAAATCA ATACGTTATT GGAAGCAAAA 
AGATTACTGG AAGTTGAGTT GGCTCAACTT GCTAAGCAGT ACAGAGGAAA TGAAGGTCAA 
ATGCGTGAGC TTCAGGATCA GCTTGAAGCG GAGCAGTATT TTTCGACACT TTACAAAACT 
CAGGTTAAGG AACTGAAGGA AGAAATTGAT GAAAAGAATA AAGAAACTCA AAGAAAAATG 
CAGGAATTGC AAAATGAGAA AGAAACACTC ACTACGCAGC TGGATTTAGC TGAAACCAAA 
GCTGAGTCGG AGCAGTTGGC ACGGGCACTT CTTGAAGAGC AGTATTTTGA GTTGAGTCAG 
GAGAGCAAAA AAGCAGCATC ACGACATAGG CAAGAAATGA CAGATAAGGA CAGCATCATA 
AGAAGGCTGG AAGAAACTAA CAGCACATTA ACCAAAGATG TTGATTTAGT AACAAAAGAA 
AATTCAGAGA TGAGTGAAAA AATGAAGAAA CAGGAGGAAG AATATAAGCT GAAAAAAGAG 
GAAGAAATAA ACAATATTAG AATGCAATAT GAGAAGAGTA TCAACAATGA GAGAACTCTG 
AAGACACAGG CTGTCAACAA ACTGGCTGAG ATAATGAATA GAAAAGATTT TAAAATAGAT 
AGAAAGAAAG CAAGCATGCA GGATTTGAGG AAGAAAGAGA AGGAAAACCG AAAGCTACAA 
TTGGAGCTTA ATCAAGAAAA AGAGAAATTC AATCAGATGG TAGTGAAATA CCAGAAGGAG 
CTTAATGAGA TGCAAGCGCA ACTAGCAGAA GAATCAACAT ACAGGAATGA GCTACAGATG 
CAGCTGGATA GTAAAGAGAG TGACATAGAA CAGCTACGAA GGAAAATTTT GGATCTCCAG 
CAAGGAATGG ATTCCACCAG TGTTTCGAGT CTACAGCCAG ATGAAATTGA TGGAAATCTT 
TCAGAATCAA GGCTGGAAGG TTGGCTTTCA ATACCAAACA AGGGAAACAT AAAGCGACAC 
GGCTGGAAGA AACAGTACGT TGTGGTGAGC AGTAAAAAGA TACTGTTCTA TAATGATGAA 
AAGGACAAGG ACCAATCTAA TCCATCCATG GTACTGGACA TAGATAAGCT GTTCCACGTC 
CGACCTGTAA CTCAAGGAGA TGTATATAGA GCAGAAACTG AAGAAATTCC TAAAATATTC 
CAGATTCTGT ATGCTAATGA GGGGGAATGC AGGAAGGATG TGGAGGTAGA ATCAGTCCAG 
CCTGCAGAGA AGACAAACTT TCTAAATCAT AAAGGCCATG AATTTATTCC AACAATATAC 
CACTTTCCAG CCAACTGTGA GGCCTGTGCC AAACCTCTTT GGCATGTCTT TAAACCCCCA 
GCTGCCCTGG AATGTCGAAG ATGCCATGTG AAGTGCCATA GAGACCACTT GGATAAAAAG 
GAGGAGTTAA TTGCACCGTG CAAAGTGAGT TACGACGTAA CTTCAGCGAG AGATATGTTG 
TTGCTGGCAT CCTGTCAAGA TGAACAGAAG AAATGGGTGA CTCATTTAGT AAAGAAGATC 
CCGAAGAACC CACCGTCTAC TTTTGTTCGT GCTTCTCCCA GAACTATGTC TACAAGATCC 
TCAGCAAACC AGTCATTCCG AAAAGCTGTT AAAAATACTT CTGGAAAAAC GAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RHOA-ROCK signalling is necessary for lateralization and differentiation of the developing sinoatrial node.
Cardiovascular research113(10)1186-1197(2017 Aug)
Vicente-Steijn R,Kelder TP,Tertoolen LG,Wisse LJ,Pijnappels DA,Poelmann RE,Schalij MJ,deRuiter MC,Gittenberger-de Groot AC,Jongbloed MRM

Metastasis of aggressive amoeboid sarcoma cells is dependent on Rho/ROCK/MLC signaling.
Cell communication and signaling : CCS1151(2013 Jul)
Kosla J,Pa?kov? D,Plach? J,Tolde O,Bicanov? K,Dvo??k M,R?sel D,Br?bek J

Downregulation of ROCK-I and ROCK-II gene expression in the cadmium-induced ventral body wall defect chick model.
Pediatric surgery international24(12)1297-301(2008 Dec)
Doi T,Puri P,Bannigan J,Thompson J

ROCK1 expression is regulated by TGFbeta3 and ALK2 during valvuloseptal endocardial cushion formation.
Anatomical record (Hoboken, N.J. : 2007)291(7)845-57(2008 Jul)
Sakabe M,Sakata H,Matsui H,Ikeda K,Yamagishi T,Nakajima Y

Rho kinase inhibitor Y27632 affects initial heart myofibrillogenesis in cultured chick blastoderm.
Developmental dynamics : an official publication of the American Association of Anatomists236(2)461-72(2007 Feb)
Sakata H,Sakabe M,Matsui H,Kawada N,Nakatani K,Ikeda K,Yamagishi T,Nakajima Y

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ROCK1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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