ACTN1 cDNA ORF clone, Gallus gallus(chicken)

The following ACTN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACTN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa27125 NM_204127.1
Latest version!
Gallus gallus actinin, alpha 1 (ACTN1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OGa150725 XM_025150528.1
Latest version!
Gallus gallus actinin, alpha 1 (ACTN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OGa150726 XM_025150529.1
Latest version!
Gallus gallus actinin, alpha 1 (ACTN1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OGa36736 XM_015287257.2
Latest version!
Gallus gallus actinin, alpha 1 (ACTN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OGa36737 XM_015287258.2
Latest version!
Gallus gallus actinin, alpha 1 (ACTN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OGa36738 XM_015287259.2
Latest version!
Gallus gallus actinin, alpha 1 (ACTN1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OGa36739 XM_015287260.1
Latest version!
Gallus gallus actinin, alpha 1 (ACTN1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OGa36740 XM_015287261.1
Latest version!
Gallus gallus actinin, alpha 1 (ACTN1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
View Old Accession Versions >>
OGa36738 XM_015287259.1 Gallus gallus actinin, alpha 1 (ACTN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OGa36736 XM_015287257.1 Gallus gallus actinin, alpha 1 (ACTN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OGa36737 XM_015287258.1 Gallus gallus actinin, alpha 1 (ACTN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa27125
    Clone ID Related Accession (Same CDS sequence) NM_204127.1
    Accession Version NM_204127.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2667bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-23
    Organism Gallus gallus(chicken)
    Product alpha-actinin-1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J03486.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J03486.1 [ECO:0000332] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGATCATC ACTACGACCC GCAGCAGACC AACGATTACA TGCAGCCGGA GGAGGACTGG 
    GACCGGGACC TGCTCCTCGA CCCGGCTTGG GAGAAGCAGC AGCGGAAGAC ATTTACAGCA
    TGGTGTAATT CTCACCTCCG CAAGGCTGGG ACACAGATTG AGAACATAGA GGAAGATTTC
    AGGGATGGTC TTAAACTCAT GTTACTTCTG GAAGTCATTT CAGGTGAACG TTTGGCTAAG
    CCCGAAAGAG GCAAAATGCG AGTGCACAAA ATATCCAACG TGAACAAGGC CTTGGATTTC
    ATTGCCAGCA AAGGAGTCAA ACTAGTATCA ATTGGAGCAG AAGAAATTGT GGATGGCAAC
    GTTAAAATGA CTCTTGGAAT GATCTGGACC ATCATCCTTC GTTTTGCCAT TCAGGATATC
    TCAGTTGAAG AGACATCTGC TAAAGAAGGA CTTTTGTTAT GGTATCAGAG GAAGACAGCT
    CCCTACAAAA ATGTAAACAT CCAGAACTTC CATATCAGTT GGAAAGATGG CCTTGGTTTC
    TGCGCTTTAA TTCACAGACA TCGTCCAGAG CTCATTGATT ACGGGAAGCT ACGAAAGGAT
    GATCCTCTCA CTAATCTAAA CACAGCCTTT GATGTAGCTG AAAAATATCT GGATATCCCC
    AAGATGCTGG ATGCAGAAGA CATTGTTGGA ACTGCCCGTC CTGACGAGAA AGCCATCATG
    ACCTATGTTT CTAGCTTCTA CCACGCCTTC TCAGGAGCCC AGAAGGCGGA GACAGCAGCA
    AATCGTATTT GCAAGGTTCT GGCAGTCAAC CAAGAAAATG AACAGCTCAT GGAAGACTAT
    GAAAAACTGG CCAGTGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC
    CGGGCACCAG AGAACACCAT GCAAGCCATG CAGCAGAAAC TGGAGGACTT CCGGGACTAC
    CGCCGCCTGC ATAAGCCCCC CAAAGTCCAA GAGAAGTGCC AACTTGAGAT CAATTTCAAC
    ACCTTGCAGA CCAAGCTACG GCTCAGCAAC AGGCCAGCCT TCATGCCTTC AGAAGGGAAA
    ATGGTTTCAG ATATAAACAA TGCCTGGGGT GGCCTAGAGC AGGCTGAGAA GGGCTATGAG
    GAGTGGTTGT TGAATGAGAT CCGGAGGCTA GAGAGGCTGG ATCACCTGGC AGAGAAGTTC
    CGGCAGAAAG CATCTATTCA TGAGTCCTGG ACAGATGGTA AGGAAGCGAT GCTGCAGCAG
    AAGGATTACG AAACTGCTAC CCTCTCGGAG ATAAAGGCCC TGCTGAAGAA ACACGAGGCC
    TTCGAGAGCG ACCTGGCTGC ACACCAGGAC CGCGTGGAAC AGATTGCTGC TATTGCACAA
    GAGCTGAATG AGCTGGACTA TTATGACTCT CCGAGTGTCA ATGCCAGGTG CCAGAAGATC
    TGTGATCAGT GGGATAATCT GGGTGCCCTA ACCCAGAAAC GTAGAGAAGC TTTAGAGAGG
    ACAGAGAAGC TACTGGAAAC AATTGACCAG TTGTACCTGG AATATGCCAA ACGAGCTGCC
    CCCTTCAATA ACTGGATGGA AGGGGCCATG GAGGACCTGC AGGACACGTT CATTGTTCAC
    ACCATTGAGG AGATCCAGGG ATTGACCACA GCCCACGAAC AGTTCAAAGC CACTTTGCCA
    GATGCCGACA AGGAGCGACA GGCCATCCTG GGAATCCACA ATGAAGTCTC AAAGATTGTC
    CAGACGTACC ACGTCAACAT GGCAGGAACG AACCCTTACA CTACAATCAC CCCACAGGAG
    ATCAATGGCA AATGGGAACA CGTGCGGCAG CTGGTTCCTA GAAGGGATCA AGCCCTAATG
    GAAGAACATG CTCGCCAGCA ACAAAATGAG CGACTTCGTA AGCAGTTTGG TGCACAAGCC
    AATGTCATTG GTCCCTGGAT TCAGACCAAG ATGGAGGAGA TTGGCCGCAT TTCAATAGAG
    ATGCACGGGA CTCTGGAGGA CCAGCTCAAC CACTTGCGGC AGTATGAGAA AAGCATTGTG
    AATTACAAAC CAAAGATCGA CCAGTTGGAA GGAGACCACC AACAGATCCA GGAGGCGCTT
    ATCTTTGACA ACAAGCACAC CAACTACACC ATGGAGCATA TTCGTGTGGG CTGGGAGCAG
    CTATTAACAA CAATTGCTAG AACCATTAAT GAAGTGGAAA ACCAGATTCT GACCCGTGAT
    GCCAAAGGAA TAAGCCAGGA ACAGATGAAT GAGTTCCGAG CCTCTTTCAA CCATTTTGAC
    AGGAAGAAGA CTGGTATGAT GGATTGTGAA GATTTCCGAG CCTGCCTTAT CTCCATGGGT
    TACAATATGG GAGAGGCAGA GTTTGCCCGC ATCATGAGCA TTGTCGATCC TAATCGCATG
    GGTGTAGTGA CGTTCCAGGC CTTCATTGAC TTCATGTCCC GGGAAACAGC AGATACAGAT
    ACTGCTGACC AGGTTATGGC TTCTTTCAAG ATCCTGGCTG GAGATAAGAA CTACATCACT
    GTGGATGAGT TACGGCGGGA GCTGCCTCCT GATCAGGCTG AGTACTGCAT TGCTAGAATG
    GCACCCTACA ATGGCCGTGA TGCTGTACCC GGTGCCCTGG ACTACATGTC GTTCTCCACG
    GCGCTCTATG GCGAAAGTGA CCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_989458.1
    CDS115..2781
    Translation

    Target ORF information:

    RefSeq Version NM_204127.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus actinin, alpha 1 (ACTN1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_204127.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGATCATC ACTACGACCC GCAGCAGACC AACGATTACA TGCAGCCGGA GGAGGACTGG 
    GACCGGGACC TGCTCCTCGA CCCGGCTTGG GAGAAGCAGC AGCGGAAGAC ATTTACAGCA
    TGGTGTAATT CTCACCTCCG CAAGGCTGGG ACACAGATTG AGAACATAGA GGAAGATTTC
    AGGGATGGTC TTAAACTCAT GTTACTTCTG GAAGTCATTT CAGGTGAACG TTTGGCTAAG
    CCCGAAAGAG GCAAAATGCG AGTGCACAAA ATATCCAACG TGAACAAGGC CTTGGATTTC
    ATTGCCAGCA AAGGAGTCAA ACTAGTATCA ATTGGAGCAG AAGAAATTGT GGATGGCAAC
    GTTAAAATGA CTCTTGGAAT GATCTGGACC ATCATCCTTC GTTTTGCCAT TCAGGATATC
    TCAGTTGAAG AGACATCTGC TAAAGAAGGA CTTTTGTTAT GGTATCAGAG GAAGACAGCT
    CCCTACAAAA ATGTAAACAT CCAGAACTTC CATATCAGTT GGAAAGATGG CCTTGGTTTC
    TGCGCTTTAA TTCACAGACA TCGTCCAGAG CTCATTGATT ACGGGAAGCT ACGAAAGGAT
    GATCCTCTCA CTAATCTAAA CACAGCCTTT GATGTAGCTG AAAAATATCT GGATATCCCC
    AAGATGCTGG ATGCAGAAGA CATTGTTGGA ACTGCCCGTC CTGACGAGAA AGCCATCATG
    ACCTATGTTT CTAGCTTCTA CCACGCCTTC TCAGGAGCCC AGAAGGCGGA GACAGCAGCA
    AATCGTATTT GCAAGGTTCT GGCAGTCAAC CAAGAAAATG AACAGCTCAT GGAAGACTAT
    GAAAAACTGG CCAGTGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC
    CGGGCACCAG AGAACACCAT GCAAGCCATG CAGCAGAAAC TGGAGGACTT CCGGGACTAC
    CGCCGCCTGC ATAAGCCCCC CAAAGTCCAA GAGAAGTGCC AACTTGAGAT CAATTTCAAC
    ACCTTGCAGA CCAAGCTACG GCTCAGCAAC AGGCCAGCCT TCATGCCTTC AGAAGGGAAA
    ATGGTTTCAG ATATAAACAA TGCCTGGGGT GGCCTAGAGC AGGCTGAGAA GGGCTATGAG
    GAGTGGTTGT TGAATGAGAT CCGGAGGCTA GAGAGGCTGG ATCACCTGGC AGAGAAGTTC
    CGGCAGAAAG CATCTATTCA TGAGTCCTGG ACAGATGGTA AGGAAGCGAT GCTGCAGCAG
    AAGGATTACG AAACTGCTAC CCTCTCGGAG ATAAAGGCCC TGCTGAAGAA ACACGAGGCC
    TTCGAGAGCG ACCTGGCTGC ACACCAGGAC CGCGTGGAAC AGATTGCTGC TATTGCACAA
    GAGCTGAATG AGCTGGACTA TTATGACTCT CCGAGTGTCA ATGCCAGGTG CCAGAAGATC
    TGTGATCAGT GGGATAATCT GGGTGCCCTA ACCCAGAAAC GTAGAGAAGC TTTAGAGAGG
    ACAGAGAAGC TACTGGAAAC AATTGACCAG TTGTACCTGG AATATGCCAA ACGAGCTGCC
    CCCTTCAATA ACTGGATGGA AGGGGCCATG GAGGACCTGC AGGACACGTT CATTGTTCAC
    ACCATTGAGG AGATCCAGGG ATTGACCACA GCCCACGAAC AGTTCAAAGC CACTTTGCCA
    GATGCCGACA AGGAGCGACA GGCCATCCTG GGAATCCACA ATGAAGTCTC AAAGATTGTC
    CAGACGTACC ACGTCAACAT GGCAGGAACG AACCCTTACA CTACAATCAC CCCACAGGAG
    ATCAATGGCA AATGGGAACA CGTGCGGCAG CTGGTTCCTA GAAGGGATCA AGCCCTAATG
    GAAGAACATG CTCGCCAGCA ACAAAATGAG CGACTTCGTA AGCAGTTTGG TGCACAAGCC
    AATGTCATTG GTCCCTGGAT TCAGACCAAG ATGGAGGAGA TTGGCCGCAT TTCAATAGAG
    ATGCACGGGA CTCTGGAGGA CCAGCTCAAC CACTTGCGGC AGTATGAGAA AAGCATTGTG
    AATTACAAAC CAAAGATCGA CCAGTTGGAA GGAGACCACC AACAGATCCA GGAGGCGCTT
    ATCTTTGACA ACAAGCACAC CAACTACACC ATGGAGCATA TTCGTGTGGG CTGGGAGCAG
    CTATTAACAA CAATTGCTAG AACCATTAAT GAAGTGGAAA ACCAGATTCT GACCCGTGAT
    GCCAAAGGAA TAAGCCAGGA ACAGATGAAT GAGTTCCGAG CCTCTTTCAA CCATTTTGAC
    AGGAAGAAGA CTGGTATGAT GGATTGTGAA GATTTCCGAG CCTGCCTTAT CTCCATGGGT
    TACAATATGG GAGAGGCAGA GTTTGCCCGC ATCATGAGCA TTGTCGATCC TAATCGCATG
    GGTGTAGTGA CGTTCCAGGC CTTCATTGAC TTCATGTCCC GGGAAACAGC AGATACAGAT
    ACTGCTGACC AGGTTATGGC TTCTTTCAAG ATCCTGGCTG GAGATAAGAA CTACATCACT
    GTGGATGAGT TACGGCGGGA GCTGCCTCCT GATCAGGCTG AGTACTGCAT TGCTAGAATG
    GCACCCTACA ATGGCCGTGA TGCTGTACCC GGTGCCCTGG ACTACATGTC GTTCTCCACG
    GCGCTCTATG GCGAAAGTGA CCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa150725
    Clone ID Related Accession (Same CDS sequence) XM_025150528.1
    Accession Version XM_025150528.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2748bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product alpha-actinin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGATCATC ACTACGACCC GCAGCAGACC AACGATTACA TGCAGCCGGA GGAGGACTGG 
    GACCGGGACC TGCTCCTCGA CCCGGCTTGG GAGAAGCAGC AGCGGAAGAC ATTTACAGCA
    TGGTGTAATT CTCACCTCCG CAAGGCTGGG ACACAGATTG AGAACATAGA GGAAGATTTC
    AGGGATGGTC TTAAACTCAT GTTACTTCTG GAAGTCATTT CAGGTGAACG TTTGGCTAAG
    CCCGAAAGAG GCAAAATGCG AGTGCACAAA ATATCCAACG TGAACAAGGC CTTGGATTTC
    ATTGCCAGCA AAGGAGTCAA ACTAGTATCA ATTGGAGCAG AAGAAATTGT GGATGGCAAC
    GTTAAAATGA CTCTTGGAAT GATCTGGACC ATCATCCTTC GTTTTGCCAT TCAGGATATC
    TCAGTTGAAG AGACATCTGC TAAAGAAGGA CTTTTGTTAT GGTGTCAGAG GAAGACAGCT
    CCCTACAAAA ATGTAAACAT CCAGAACTTC CATATCAGTT GGAAAGATGG CCTTGGTTTC
    TGCGCTTTAA TTCACAGACA TCGTCCAGAG CTCATTGATT ACGGGAAGCT ACGAAAGGAT
    GATCCTCTCA CTAATCTAAA CACAGCCTTT GATGTAGCTG AAAAATATCT GGATATCCCC
    AAGATGCTGG ATGCAGAAGA TATTATAGGC ACGCTAAGGC CGGATGAGAA GGCCATAATG
    ACCTATGTTT CATGTTACTA CCACGCCTTC TCAGGGGCAC AGAAGGCGGA GACAGCAGCA
    AATCGTATTT GCAAGGTTCT GGCAGTCAAC CAAGAGAATG AACAGCTCAT GGAAGACTAT
    GAAAAACTGG CCAGTGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC
    CGGGCACCAG AGAACACCAT GCAAGCCATG CAGCAGAAAC TGGAGGACTT CCGGGACTAC
    CGCCGCCTGC ATAAGCCCCC CAAAGTCCAA GAGAAGTGCC AACTTGAGAT CAATTTCAAC
    ACCTTGCAGA CCAAGCTACG GCTCAGCAAC AGGCCGGCCT TCATGCCTTC AGAAGGGAAA
    ATGGTTTCAG ATATAAACAA TGCCTGGGGT GGCCTAGAGC AGGCTGAGAA GGGCTATGAG
    GAGTGGTTGT TGAATGAGAT CCGGAGGCTA GAGAGGCTGG ATCACCTGGC AGAGAAGTTC
    CGGCAGAAAG CATCTATTCA CGAGTCCTGG ACAGATGGTA AGGAAGCGAT GCTGCAGCAG
    AAGGATTATG AAACTGCTAC CCTCTCGGAG ATAAAGGCCC TGCTGAAGAA ACACGAGGCC
    TTTGAGAGCG ACCTGGCTGC ACACCAGGAC CGCGTGGAAC AGATTGCTGC TATTGCACAA
    GAGCTGAATG AGCTGGACTA TTATGACTCT CCGAGTGTCA ATGCCAGGTG CCAGAAGATC
    TGTGATCAGT GGGATAATCT GGGTGCCCTA ACCCAGAAAC GTAGAGAAGC TTTAGAGAGG
    ACAGAGAAGC TACTGGAAAC AATTGACCAG TTGTACCTGG AATATGCCAA ACGAGCTGCC
    CCCTTCAATA ACTGGATGGA AGGGGCCATG GAGGACCTGC AGGACACGTT CATTGTTCAC
    ACCATTGAGG AGATCCAGGG ATTGACCACA GCCCACGAAC AGTTCAAAGC CACTTTGCCA
    GATGCCGACA AGGAGCGACA GGCCATCCTG GGAATCCACA ATGAAGTCTC AAAGATTGTC
    CAGACGTACC ACGTCAACAT GGCAGGAACG AACCCTTACA CTACAATCAC CCCACAGGAG
    ATCAATGGCA AATGGGAACA CGTGCGGCAG CTGGTTCCTA GAAGGGATCA AGCCCTAATG
    GAAGAACATG CTCGCCAGCA GCAAAATGAG CGACTTCGTA AGCAGTTTGG TGCACAAGCC
    AATGTCATTG GTCCCTGGAT TCAGACCAAG ATGGAGGAGA TTGGCCGCAT TTCAATAGAG
    ATGCACGGGA CTCTGGAGGA CCAGCTCAAC CACTTGCGGC AGTATGAGAA AAGCATTGTG
    AATTACAAAC CAAAGATCGA CCAGTTGGAA GGAGACCACC AACAGATCCA GGAGGCGCTT
    ATCTTTGACA ACAAGCACAC CAACTACACC ATGGAGCATA TTCGTGTGGG CTGGGAGCAG
    CTATTAACAA CAATTGCTAG AACCATTAAT GAAGTGGAAA ACCAGATTCT GACCCGTGAT
    GCCAAAGGAA TAAGCCAGGA ACAGATGAAT GAGTTCCGAG CCTCTTTCAA CCATTTTGAC
    AGGGACCACT CCGGCACACT TGGCCCTGAG GAATTCAAAG CCTGTCTCAT CAGCTTGGGT
    TACGATATTG GAAACGATGC ACAGAAGAAG ACTGGTATGA TGGATTGTGA AGATTTCCGA
    GCCTGCCTTA TCTCCATGGG TTACAATATG GGAGAGGCAG AGTTTGCCCG CATCATGAGC
    ATTGTCGATC CTAATCGCAT GGGTGTAGTG ACGTTCCAGG CCTTCATTGA CTTCATGTCC
    CGGGAAACAG CAGATACAGA TACTGCTGAC CAGGTTATGG CTTCTTTCAA GATCCTGGCT
    GGAGATAAGA ACTACATCAC TGTGGATGAG TTACGGCGGG AGCTGCCTCC TGATCAGGCT
    GAGTACTGCA TTGCTAGAAT GGCACCCTAC AATGGCCGTG ATGCTGTACC CGGTGCCCTG
    GACTACATGT CGTTCTCCAC GGCGCTCTAT GGCGAAAGTG ACCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025006296.1
    CDS69..2816
    Translation

    Target ORF information:

    RefSeq Version XM_025150528.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus actinin, alpha 1 (ACTN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025150528.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGATCATC ACTACGACCC GCAGCAGACC AACGATTACA TGCAGCCGGA GGAGGACTGG 
    GACCGGGACC TGCTCCTCGA CCCGGCTTGG GAGAAGCAGC AGCGGAAGAC ATTTACAGCA
    TGGTGTAATT CTCACCTCCG CAAGGCTGGG ACACAGATTG AGAACATAGA GGAAGATTTC
    AGGGATGGTC TTAAACTCAT GTTACTTCTG GAAGTCATTT CAGGTGAACG TTTGGCTAAG
    CCCGAAAGAG GCAAAATGCG AGTGCACAAA ATATCCAACG TGAACAAGGC CTTGGATTTC
    ATTGCCAGCA AAGGAGTCAA ACTAGTATCA ATTGGAGCAG AAGAAATTGT GGATGGCAAC
    GTTAAAATGA CTCTTGGAAT GATCTGGACC ATCATCCTTC GTTTTGCCAT TCAGGATATC
    TCAGTTGAAG AGACATCTGC TAAAGAAGGA CTTTTGTTAT GGTGTCAGAG GAAGACAGCT
    CCCTACAAAA ATGTAAACAT CCAGAACTTC CATATCAGTT GGAAAGATGG CCTTGGTTTC
    TGCGCTTTAA TTCACAGACA TCGTCCAGAG CTCATTGATT ACGGGAAGCT ACGAAAGGAT
    GATCCTCTCA CTAATCTAAA CACAGCCTTT GATGTAGCTG AAAAATATCT GGATATCCCC
    AAGATGCTGG ATGCAGAAGA TATTATAGGC ACGCTAAGGC CGGATGAGAA GGCCATAATG
    ACCTATGTTT CATGTTACTA CCACGCCTTC TCAGGGGCAC AGAAGGCGGA GACAGCAGCA
    AATCGTATTT GCAAGGTTCT GGCAGTCAAC CAAGAGAATG AACAGCTCAT GGAAGACTAT
    GAAAAACTGG CCAGTGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC
    CGGGCACCAG AGAACACCAT GCAAGCCATG CAGCAGAAAC TGGAGGACTT CCGGGACTAC
    CGCCGCCTGC ATAAGCCCCC CAAAGTCCAA GAGAAGTGCC AACTTGAGAT CAATTTCAAC
    ACCTTGCAGA CCAAGCTACG GCTCAGCAAC AGGCCGGCCT TCATGCCTTC AGAAGGGAAA
    ATGGTTTCAG ATATAAACAA TGCCTGGGGT GGCCTAGAGC AGGCTGAGAA GGGCTATGAG
    GAGTGGTTGT TGAATGAGAT CCGGAGGCTA GAGAGGCTGG ATCACCTGGC AGAGAAGTTC
    CGGCAGAAAG CATCTATTCA CGAGTCCTGG ACAGATGGTA AGGAAGCGAT GCTGCAGCAG
    AAGGATTATG AAACTGCTAC CCTCTCGGAG ATAAAGGCCC TGCTGAAGAA ACACGAGGCC
    TTTGAGAGCG ACCTGGCTGC ACACCAGGAC CGCGTGGAAC AGATTGCTGC TATTGCACAA
    GAGCTGAATG AGCTGGACTA TTATGACTCT CCGAGTGTCA ATGCCAGGTG CCAGAAGATC
    TGTGATCAGT GGGATAATCT GGGTGCCCTA ACCCAGAAAC GTAGAGAAGC TTTAGAGAGG
    ACAGAGAAGC TACTGGAAAC AATTGACCAG TTGTACCTGG AATATGCCAA ACGAGCTGCC
    CCCTTCAATA ACTGGATGGA AGGGGCCATG GAGGACCTGC AGGACACGTT CATTGTTCAC
    ACCATTGAGG AGATCCAGGG ATTGACCACA GCCCACGAAC AGTTCAAAGC CACTTTGCCA
    GATGCCGACA AGGAGCGACA GGCCATCCTG GGAATCCACA ATGAAGTCTC AAAGATTGTC
    CAGACGTACC ACGTCAACAT GGCAGGAACG AACCCTTACA CTACAATCAC CCCACAGGAG
    ATCAATGGCA AATGGGAACA CGTGCGGCAG CTGGTTCCTA GAAGGGATCA AGCCCTAATG
    GAAGAACATG CTCGCCAGCA GCAAAATGAG CGACTTCGTA AGCAGTTTGG TGCACAAGCC
    AATGTCATTG GTCCCTGGAT TCAGACCAAG ATGGAGGAGA TTGGCCGCAT TTCAATAGAG
    ATGCACGGGA CTCTGGAGGA CCAGCTCAAC CACTTGCGGC AGTATGAGAA AAGCATTGTG
    AATTACAAAC CAAAGATCGA CCAGTTGGAA GGAGACCACC AACAGATCCA GGAGGCGCTT
    ATCTTTGACA ACAAGCACAC CAACTACACC ATGGAGCATA TTCGTGTGGG CTGGGAGCAG
    CTATTAACAA CAATTGCTAG AACCATTAAT GAAGTGGAAA ACCAGATTCT GACCCGTGAT
    GCCAAAGGAA TAAGCCAGGA ACAGATGAAT GAGTTCCGAG CCTCTTTCAA CCATTTTGAC
    AGGGACCACT CCGGCACACT TGGCCCTGAG GAATTCAAAG CCTGTCTCAT CAGCTTGGGT
    TACGATATTG GAAACGATGC ACAGAAGAAG ACTGGTATGA TGGATTGTGA AGATTTCCGA
    GCCTGCCTTA TCTCCATGGG TTACAATATG GGAGAGGCAG AGTTTGCCCG CATCATGAGC
    ATTGTCGATC CTAATCGCAT GGGTGTAGTG ACGTTCCAGG CCTTCATTGA CTTCATGTCC
    CGGGAAACAG CAGATACAGA TACTGCTGAC CAGGTTATGG CTTCTTTCAA GATCCTGGCT
    GGAGATAAGA ACTACATCAC TGTGGATGAG TTACGGCGGG AGCTGCCTCC TGATCAGGCT
    GAGTACTGCA TTGCTAGAAT GGCACCCTAC AATGGCCGTG ATGCTGTACC CGGTGCCCTG
    GACTACATGT CGTTCTCCAC GGCGCTCTAT GGCGAAAGTG ACCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa150726
    Clone ID Related Accession (Same CDS sequence) XM_025150529.1
    Accession Version XM_025150529.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product alpha-actinin-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGATCATC ACTACGACCC GCAGCAGACC AACGATTACA TGCAGCCGGA GGAGGACTGG 
    GACCGGGACC TGCTCCTCGA CCCGGCTTGG GAGAAGCAGC AGCGGAAGAC ATTTACAGCA
    TGGTGTAATT CTCACCTCCG CAAGGCTGGG ACACAGATTG AGAACATAGA GGAAGATTTC
    AGGGATGGTC TTAAACTCAT GTTACTTCTG GAAGTCATTT CAGGTGAACG TTTGGCTAAG
    CCCGAAAGAG GCAAAATGCG AGTGCACAAA ATATCCAACG TGAACAAGGC CTTGGATTTC
    ATTGCCAGCA AAGGAGTCAA ACTAGTATCA ATTGGAGCAG AAGAAATTGT GGATGGCAAC
    GTTAAAATGA CTCTTGGAAT GATCTGGACC ATCATCCTTC GTTTTGCCAT TCAGGATATC
    TCAGTTGAAG AGACATCTGC TAAAGAAGGA CTTTTGTTAT GGTGTCAGAG GAAGACAGCT
    CCCTACAAAA ATGTAAACAT CCAGAACTTC CATATCAGTT GGAAAGATGG CCTTGGTTTC
    TGCGCTTTAA TTCACAGACA TCGTCCAGAG CTCATTGATT ACGGGAAGCT ACGAAAGGAT
    GATCCTCTCA CTAATCTAAA CACAGCCTTT GATGTAGCTG AAAAATATCT GGATATCCCC
    AAGATGCTGG ATGCAGAAGA TATTATAGGC ACGCTAAGGC CGGATGAGAA GGCCATAATG
    ACCTATGTTT CATGTTACTA CCACGCCTTC TCAGGGGCAC AGAAGGCGGA GACAGCAGCA
    AATCGTATTT GCAAGGTTCT GGCAGTCAAC CAAGAGAATG AACAGCTCAT GGAAGACTAT
    GAAAAACTGG CCAGTGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC
    CGGGCACCAG AGAACACCAT GCAAGCCATG CAGCAGAAAC TGGAGGACTT CCGGGACTAC
    CGCCGCCTGC ATAAGCCCCC CAAAGTCCAA GAGAAGTGCC AACTTGAGAT CAATTTCAAC
    ACCTTGCAGA CCAAGCTACG GCTCAGCAAC AGGCCGGCCT TCATGCCTTC AGAAGGGAAA
    ATGGTTTCAG ATATAAACAA TGCCTGGGGT GGCCTAGAGC AGGCTGAGAA GGGCTATGAG
    GAGTGGTTGT TGAATGAGAT CCGGAGGCTA GAGAGGCTGG ATCACCTGGC AGAGAAGTTC
    CGGCAGAAAG CATCTATTCA CGAGTCCTGG ACAGATGGTA AGGAAGCGAT GCTGCAGCAG
    AAGGATTATG AAACTGCTAC CCTCTCGGAG ATAAAGGCCC TGCTGAAGAA ACACGAGGCC
    TTTGAGAGCG ACCTGGCTGC ACACCAGGAC CGCGTGGAAC AGATTGCTGC TATTGCACAA
    GAGCTGAATG AGCTGGACTA TTATGACTCT CCGAGTGTCA ATGCCAGGTG CCAGAAGATC
    TGTGATCAGT GGGATAATCT GGGTGCCCTA ACCCAGAAAC GTAGAGAAGC TTTAGAGAGG
    ACAGAGAAGC TACTGGAAAC AATTGACCAG TTGTACCTGG AATATGCCAA ACGAGCTGCC
    CCCTTCAATA ACTGGATGGA AGGGGCCATG GAGGACCTGC AGGACACGTT CATTGTTCAC
    ACCATTGAGG AGATCCAGGG ATTGACCACA GCCCACGAAC AGTTCAAAGC CACTTTGCCA
    GATGCCGACA AGGAGCGACA GGCCATCCTG GGAATCCACA ATGAAGTCTC AAAGATTGTC
    CAGACGTACC ACGTCAACAT GGCAGGAACG AACCCTTACA CTACAATCAC CCCACAGGAG
    ATCAATGGCA AATGGGAACA CGTGCGGCAG CTGGTTCCTA GAAGGGATCA AGCCCTAATG
    GAAGAACATG CTCGCCAGCA GCAAAATGAG CGACTTCGTA AGCAGTTTGG TGCACAAGCC
    AATGTCATTG GTCCCTGGAT TCAGACCAAG ATGGAGGAGA TTGGCCGCAT TTCAATAGAG
    ATGCACGGGA CTCTGGAGGA CCAGCTCAAC CACTTGCGGC AGTATGAGAA AAGCATTGTG
    AATTACAAAC CAAAGATCGA CCAGTTGGAA GGAGACCACC AACAGATCCA GGAGGCGCTT
    ATCTTTGACA ACAAGCACAC CAACTACACC ATGGAGCATA TTCGTGTGGG CTGGGAGCAG
    CTATTAACAA CAATTGCTAG AACCATTAAT GAAGTGGAAA ACCAGATTCT GACCCGTGAT
    GCCAAAGGAA TAAGCCAGGA ACAGATGAAT GAGTTCCGAG CCTCTTTCAA CCATTTTGAC
    AGGGACCACT CCGGCACACT TGGCCCTGAG GAATTCAAAG CCTGTCTCAT CAGCTTGGGT
    TACGATATTG GAAACGATGC ACAGGGAGAG GCAGAGTTTG CCCGCATCAT GAGCATTGTC
    GATCCTAATC GCATGGGTGT AGTGACGTTC CAGGCCTTCA TTGACTTCAT GTCCCGGGAA
    ACAGCAGATA CAGATACTGC TGACCAGGTT ATGGCTTCTT TCAAGATCCT GGCTGGAGAT
    AAGAACTACA TCACTGTGGA TGAGTTACGG CGGGAGCTGC CTCCTGATCA GGCTGAGTAC
    TGCATTGCTA GAATGGCACC CTACAATGGC CGTGATGCTG TACCCGGTGC CCTGGACTAC
    ATGTCGTTCT CCACGGCGCT CTATGGCGAA AGTGACCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025006297.1
    CDS69..2750
    Translation

    Target ORF information:

    RefSeq Version XM_025150529.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus actinin, alpha 1 (ACTN1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025150529.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGATCATC ACTACGACCC GCAGCAGACC AACGATTACA TGCAGCCGGA GGAGGACTGG 
    GACCGGGACC TGCTCCTCGA CCCGGCTTGG GAGAAGCAGC AGCGGAAGAC ATTTACAGCA
    TGGTGTAATT CTCACCTCCG CAAGGCTGGG ACACAGATTG AGAACATAGA GGAAGATTTC
    AGGGATGGTC TTAAACTCAT GTTACTTCTG GAAGTCATTT CAGGTGAACG TTTGGCTAAG
    CCCGAAAGAG GCAAAATGCG AGTGCACAAA ATATCCAACG TGAACAAGGC CTTGGATTTC
    ATTGCCAGCA AAGGAGTCAA ACTAGTATCA ATTGGAGCAG AAGAAATTGT GGATGGCAAC
    GTTAAAATGA CTCTTGGAAT GATCTGGACC ATCATCCTTC GTTTTGCCAT TCAGGATATC
    TCAGTTGAAG AGACATCTGC TAAAGAAGGA CTTTTGTTAT GGTGTCAGAG GAAGACAGCT
    CCCTACAAAA ATGTAAACAT CCAGAACTTC CATATCAGTT GGAAAGATGG CCTTGGTTTC
    TGCGCTTTAA TTCACAGACA TCGTCCAGAG CTCATTGATT ACGGGAAGCT ACGAAAGGAT
    GATCCTCTCA CTAATCTAAA CACAGCCTTT GATGTAGCTG AAAAATATCT GGATATCCCC
    AAGATGCTGG ATGCAGAAGA TATTATAGGC ACGCTAAGGC CGGATGAGAA GGCCATAATG
    ACCTATGTTT CATGTTACTA CCACGCCTTC TCAGGGGCAC AGAAGGCGGA GACAGCAGCA
    AATCGTATTT GCAAGGTTCT GGCAGTCAAC CAAGAGAATG AACAGCTCAT GGAAGACTAT
    GAAAAACTGG CCAGTGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC
    CGGGCACCAG AGAACACCAT GCAAGCCATG CAGCAGAAAC TGGAGGACTT CCGGGACTAC
    CGCCGCCTGC ATAAGCCCCC CAAAGTCCAA GAGAAGTGCC AACTTGAGAT CAATTTCAAC
    ACCTTGCAGA CCAAGCTACG GCTCAGCAAC AGGCCGGCCT TCATGCCTTC AGAAGGGAAA
    ATGGTTTCAG ATATAAACAA TGCCTGGGGT GGCCTAGAGC AGGCTGAGAA GGGCTATGAG
    GAGTGGTTGT TGAATGAGAT CCGGAGGCTA GAGAGGCTGG ATCACCTGGC AGAGAAGTTC
    CGGCAGAAAG CATCTATTCA CGAGTCCTGG ACAGATGGTA AGGAAGCGAT GCTGCAGCAG
    AAGGATTATG AAACTGCTAC CCTCTCGGAG ATAAAGGCCC TGCTGAAGAA ACACGAGGCC
    TTTGAGAGCG ACCTGGCTGC ACACCAGGAC CGCGTGGAAC AGATTGCTGC TATTGCACAA
    GAGCTGAATG AGCTGGACTA TTATGACTCT CCGAGTGTCA ATGCCAGGTG CCAGAAGATC
    TGTGATCAGT GGGATAATCT GGGTGCCCTA ACCCAGAAAC GTAGAGAAGC TTTAGAGAGG
    ACAGAGAAGC TACTGGAAAC AATTGACCAG TTGTACCTGG AATATGCCAA ACGAGCTGCC
    CCCTTCAATA ACTGGATGGA AGGGGCCATG GAGGACCTGC AGGACACGTT CATTGTTCAC
    ACCATTGAGG AGATCCAGGG ATTGACCACA GCCCACGAAC AGTTCAAAGC CACTTTGCCA
    GATGCCGACA AGGAGCGACA GGCCATCCTG GGAATCCACA ATGAAGTCTC AAAGATTGTC
    CAGACGTACC ACGTCAACAT GGCAGGAACG AACCCTTACA CTACAATCAC CCCACAGGAG
    ATCAATGGCA AATGGGAACA CGTGCGGCAG CTGGTTCCTA GAAGGGATCA AGCCCTAATG
    GAAGAACATG CTCGCCAGCA GCAAAATGAG CGACTTCGTA AGCAGTTTGG TGCACAAGCC
    AATGTCATTG GTCCCTGGAT TCAGACCAAG ATGGAGGAGA TTGGCCGCAT TTCAATAGAG
    ATGCACGGGA CTCTGGAGGA CCAGCTCAAC CACTTGCGGC AGTATGAGAA AAGCATTGTG
    AATTACAAAC CAAAGATCGA CCAGTTGGAA GGAGACCACC AACAGATCCA GGAGGCGCTT
    ATCTTTGACA ACAAGCACAC CAACTACACC ATGGAGCATA TTCGTGTGGG CTGGGAGCAG
    CTATTAACAA CAATTGCTAG AACCATTAAT GAAGTGGAAA ACCAGATTCT GACCCGTGAT
    GCCAAAGGAA TAAGCCAGGA ACAGATGAAT GAGTTCCGAG CCTCTTTCAA CCATTTTGAC
    AGGGACCACT CCGGCACACT TGGCCCTGAG GAATTCAAAG CCTGTCTCAT CAGCTTGGGT
    TACGATATTG GAAACGATGC ACAGGGAGAG GCAGAGTTTG CCCGCATCAT GAGCATTGTC
    GATCCTAATC GCATGGGTGT AGTGACGTTC CAGGCCTTCA TTGACTTCAT GTCCCGGGAA
    ACAGCAGATA CAGATACTGC TGACCAGGTT ATGGCTTCTT TCAAGATCCT GGCTGGAGAT
    AAGAACTACA TCACTGTGGA TGAGTTACGG CGGGAGCTGC CTCCTGATCA GGCTGAGTAC
    TGCATTGCTA GAATGGCACC CTACAATGGC CGTGATGCTG TACCCGGTGC CCTGGACTAC
    ATGTCGTTCT CCACGGCGCT CTATGGCGAA AGTGACCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa36736
    Clone ID Related Accession (Same CDS sequence) XM_015287257.1 , XM_015287257.2
    Accession Version XM_015287257.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2748bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product alpha-actinin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGATCATC ACTACGACCC GCAGCAGACC AACGATTACA TGCAGCCGGA GGAGGACTGG 
    GACCGGGACC TGCTCCTCGA CCCGGCTTGG GAGAAGCAGC AGCGGAAGAC ATTTACAGCA
    TGGTGTAATT CTCACCTCCG CAAGGCTGGG ACACAGATTG AGAACATAGA GGAAGATTTC
    AGGGATGGTC TTAAACTCAT GTTACTTCTG GAAGTCATTT CAGGTGAACG TTTGGCTAAG
    CCCGAAAGAG GCAAAATGCG AGTGCACAAA ATATCCAACG TGAACAAGGC CTTGGATTTC
    ATTGCCAGCA AAGGAGTCAA ACTAGTATCA ATTGGAGCAG AAGAAATTGT GGATGGCAAC
    GTTAAAATGA CTCTTGGAAT GATCTGGACC ATCATCCTTC GTTTTGCCAT TCAGGATATC
    TCAGTTGAAG AGACATCTGC TAAAGAAGGA CTTTTGTTAT GGTGTCAGAG GAAGACAGCT
    CCCTACAAAA ATGTAAACAT CCAGAACTTC CATATCAGTT GGAAAGATGG CCTTGGTTTC
    TGCGCTTTAA TTCACAGACA TCGTCCAGAG CTCATTGATT ACGGGAAGCT ACGAAAGGAT
    GATCCTCTCA CTAATCTAAA CACAGCCTTT GATGTAGCTG AAAAATATCT GGATATCCCC
    AAGATGCTGG ATGCAGAAGA CATTGTTGGA ACTGCCCGTC CTGACGAGAA AGCCATCATG
    ACCTATGTTT CTAGCTTCTA CCACGCCTTC TCAGGAGCCC AGAAGGCGGA GACAGCAGCA
    AATCGTATTT GCAAGGTTCT GGCAGTCAAC CAAGAGAATG AACAGCTCAT GGAAGACTAT
    GAAAAACTGG CCAGTGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC
    CGGGCACCAG AGAACACCAT GCAAGCCATG CAGCAGAAAC TGGAGGACTT CCGGGACTAC
    CGCCGCCTGC ATAAGCCCCC CAAAGTCCAA GAGAAGTGCC AACTTGAGAT CAATTTCAAC
    ACCTTGCAGA CCAAGCTACG GCTCAGCAAC AGGCCGGCCT TCATGCCTTC AGAAGGGAAA
    ATGGTTTCAG ATATAAACAA TGCCTGGGGT GGCCTAGAGC AGGCTGAGAA GGGCTATGAG
    GAGTGGTTGT TGAATGAGAT CCGGAGGCTA GAGAGGCTGG ATCACCTGGC AGAGAAGTTC
    CGGCAGAAAG CATCTATTCA CGAGTCCTGG ACAGATGGTA AGGAAGCGAT GCTGCAGCAG
    AAGGATTATG AAACTGCTAC CCTCTCGGAG ATAAAGGCCC TGCTGAAGAA ACACGAGGCC
    TTTGAGAGCG ACCTGGCTGC ACACCAGGAC CGCGTGGAAC AGATTGCTGC TATTGCACAA
    GAGCTGAATG AGCTGGACTA TTATGACTCT CCGAGTGTCA ATGCCAGGTG CCAGAAGATC
    TGTGATCAGT GGGATAATCT GGGTGCCCTA ACCCAGAAAC GTAGAGAAGC TTTAGAGAGG
    ACAGAGAAGC TACTGGAAAC AATTGACCAG TTGTACCTGG AATATGCCAA ACGAGCTGCC
    CCCTTCAATA ACTGGATGGA AGGGGCCATG GAGGACCTGC AGGACACGTT CATTGTTCAC
    ACCATTGAGG AGATCCAGGG ATTGACCACA GCCCACGAAC AGTTCAAAGC CACTTTGCCA
    GATGCCGACA AGGAGCGACA GGCCATCCTG GGAATCCACA ATGAAGTCTC AAAGATTGTC
    CAGACGTACC ACGTCAACAT GGCAGGAACG AACCCTTACA CTACAATCAC CCCACAGGAG
    ATCAATGGCA AATGGGAACA CGTGCGGCAG CTGGTTCCTA GAAGGGATCA AGCCCTAATG
    GAAGAACATG CTCGCCAGCA GCAAAATGAG CGACTTCGTA AGCAGTTTGG TGCACAAGCC
    AATGTCATTG GTCCCTGGAT TCAGACCAAG ATGGAGGAGA TTGGCCGCAT TTCAATAGAG
    ATGCACGGGA CTCTGGAGGA CCAGCTCAAC CACTTGCGGC AGTATGAGAA AAGCATTGTG
    AATTACAAAC CAAAGATCGA CCAGTTGGAA GGAGACCACC AACAGATCCA GGAGGCGCTT
    ATCTTTGACA ACAAGCACAC CAACTACACC ATGGAGCATA TTCGTGTGGG CTGGGAGCAG
    CTATTAACAA CAATTGCTAG AACCATTAAT GAAGTGGAAA ACCAGATTCT GACCCGTGAT
    GCCAAAGGAA TAAGCCAGGA ACAGATGAAT GAGTTCCGAG CCTCTTTCAA CCATTTTGAC
    AGGGACCACT CCGGCACACT TGGCCCTGAG GAATTCAAAG CCTGTCTCAT CAGCTTGGGT
    TACGATATTG GAAACGATGC ACAGAAGAAG ACTGGTATGA TGGATTGTGA AGATTTCCGA
    GCCTGCCTTA TCTCCATGGG TTACAATATG GGAGAGGCAG AGTTTGCCCG CATCATGAGC
    ATTGTCGATC CTAATCGCAT GGGTGTAGTG ACGTTCCAGG CCTTCATTGA CTTCATGTCC
    CGGGAAACAG CAGATACAGA TACTGCTGAC CAGGTTATGG CTTCTTTCAA GATCCTGGCT
    GGAGATAAGA ACTACATCAC TGTGGATGAG TTACGGCGGG AGCTGCCTCC TGATCAGGCT
    GAGTACTGCA TTGCTAGAAT GGCACCCTAC AATGGCCGTG ATGCTGTACC CGGTGCCCTG
    GACTACATGT CGTTCTCCAC GGCGCTCTAT GGCGAAAGTG ACCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015142743.1
    CDS1..2748
    Translation

    Target ORF information:

    RefSeq Version XM_015287257.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus actinin, alpha 1 (ACTN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287257.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGATCATC ACTACGACCC GCAGCAGACC AACGATTACA TGCAGCCGGA GGAGGACTGG 
    GACCGGGACC TGCTCCTCGA CCCGGCTTGG GAGAAGCAGC AGCGGAAGAC ATTTACAGCA
    TGGTGTAATT CTCACCTCCG CAAGGCTGGG ACACAGATTG AGAACATAGA GGAAGATTTC
    AGGGATGGTC TTAAACTCAT GTTACTTCTG GAAGTCATTT CAGGTGAACG TTTGGCTAAG
    CCCGAAAGAG GCAAAATGCG AGTGCACAAA ATATCCAACG TGAACAAGGC CTTGGATTTC
    ATTGCCAGCA AAGGAGTCAA ACTAGTATCA ATTGGAGCAG AAGAAATTGT GGATGGCAAC
    GTTAAAATGA CTCTTGGAAT GATCTGGACC ATCATCCTTC GTTTTGCCAT TCAGGATATC
    TCAGTTGAAG AGACATCTGC TAAAGAAGGA CTTTTGTTAT GGTGTCAGAG GAAGACAGCT
    CCCTACAAAA ATGTAAACAT CCAGAACTTC CATATCAGTT GGAAAGATGG CCTTGGTTTC
    TGCGCTTTAA TTCACAGACA TCGTCCAGAG CTCATTGATT ACGGGAAGCT ACGAAAGGAT
    GATCCTCTCA CTAATCTAAA CACAGCCTTT GATGTAGCTG AAAAATATCT GGATATCCCC
    AAGATGCTGG ATGCAGAAGA CATTGTTGGA ACTGCCCGTC CTGACGAGAA AGCCATCATG
    ACCTATGTTT CTAGCTTCTA CCACGCCTTC TCAGGAGCCC AGAAGGCGGA GACAGCAGCA
    AATCGTATTT GCAAGGTTCT GGCAGTCAAC CAAGAGAATG AACAGCTCAT GGAAGACTAT
    GAAAAACTGG CCAGTGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC
    CGGGCACCAG AGAACACCAT GCAAGCCATG CAGCAGAAAC TGGAGGACTT CCGGGACTAC
    CGCCGCCTGC ATAAGCCCCC CAAAGTCCAA GAGAAGTGCC AACTTGAGAT CAATTTCAAC
    ACCTTGCAGA CCAAGCTACG GCTCAGCAAC AGGCCGGCCT TCATGCCTTC AGAAGGGAAA
    ATGGTTTCAG ATATAAACAA TGCCTGGGGT GGCCTAGAGC AGGCTGAGAA GGGCTATGAG
    GAGTGGTTGT TGAATGAGAT CCGGAGGCTA GAGAGGCTGG ATCACCTGGC AGAGAAGTTC
    CGGCAGAAAG CATCTATTCA CGAGTCCTGG ACAGATGGTA AGGAAGCGAT GCTGCAGCAG
    AAGGATTATG AAACTGCTAC CCTCTCGGAG ATAAAGGCCC TGCTGAAGAA ACACGAGGCC
    TTTGAGAGCG ACCTGGCTGC ACACCAGGAC CGCGTGGAAC AGATTGCTGC TATTGCACAA
    GAGCTGAATG AGCTGGACTA TTATGACTCT CCGAGTGTCA ATGCCAGGTG CCAGAAGATC
    TGTGATCAGT GGGATAATCT GGGTGCCCTA ACCCAGAAAC GTAGAGAAGC TTTAGAGAGG
    ACAGAGAAGC TACTGGAAAC AATTGACCAG TTGTACCTGG AATATGCCAA ACGAGCTGCC
    CCCTTCAATA ACTGGATGGA AGGGGCCATG GAGGACCTGC AGGACACGTT CATTGTTCAC
    ACCATTGAGG AGATCCAGGG ATTGACCACA GCCCACGAAC AGTTCAAAGC CACTTTGCCA
    GATGCCGACA AGGAGCGACA GGCCATCCTG GGAATCCACA ATGAAGTCTC AAAGATTGTC
    CAGACGTACC ACGTCAACAT GGCAGGAACG AACCCTTACA CTACAATCAC CCCACAGGAG
    ATCAATGGCA AATGGGAACA CGTGCGGCAG CTGGTTCCTA GAAGGGATCA AGCCCTAATG
    GAAGAACATG CTCGCCAGCA GCAAAATGAG CGACTTCGTA AGCAGTTTGG TGCACAAGCC
    AATGTCATTG GTCCCTGGAT TCAGACCAAG ATGGAGGAGA TTGGCCGCAT TTCAATAGAG
    ATGCACGGGA CTCTGGAGGA CCAGCTCAAC CACTTGCGGC AGTATGAGAA AAGCATTGTG
    AATTACAAAC CAAAGATCGA CCAGTTGGAA GGAGACCACC AACAGATCCA GGAGGCGCTT
    ATCTTTGACA ACAAGCACAC CAACTACACC ATGGAGCATA TTCGTGTGGG CTGGGAGCAG
    CTATTAACAA CAATTGCTAG AACCATTAAT GAAGTGGAAA ACCAGATTCT GACCCGTGAT
    GCCAAAGGAA TAAGCCAGGA ACAGATGAAT GAGTTCCGAG CCTCTTTCAA CCATTTTGAC
    AGGGACCACT CCGGCACACT TGGCCCTGAG GAATTCAAAG CCTGTCTCAT CAGCTTGGGT
    TACGATATTG GAAACGATGC ACAGAAGAAG ACTGGTATGA TGGATTGTGA AGATTTCCGA
    GCCTGCCTTA TCTCCATGGG TTACAATATG GGAGAGGCAG AGTTTGCCCG CATCATGAGC
    ATTGTCGATC CTAATCGCAT GGGTGTAGTG ACGTTCCAGG CCTTCATTGA CTTCATGTCC
    CGGGAAACAG CAGATACAGA TACTGCTGAC CAGGTTATGG CTTCTTTCAA GATCCTGGCT
    GGAGATAAGA ACTACATCAC TGTGGATGAG TTACGGCGGG AGCTGCCTCC TGATCAGGCT
    GAGTACTGCA TTGCTAGAAT GGCACCCTAC AATGGCCGTG ATGCTGTACC CGGTGCCCTG
    GACTACATGT CGTTCTCCAC GGCGCTCTAT GGCGAAAGTG ACCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa36736
    Clone ID Related Accession (Same CDS sequence) XM_015287257.1 , XM_015287257.2
    Accession Version XM_015287257.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2748bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product alpha-actinin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287257.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGATCATC ACTACGACCC GCAGCAGACC AACGATTACA TGCAGCCGGA GGAGGACTGG 
    GACCGGGACC TGCTCCTCGA CCCGGCTTGG GAGAAGCAGC AGCGGAAGAC ATTTACAGCA
    TGGTGTAATT CTCACCTCCG CAAGGCTGGG ACACAGATTG AGAACATAGA GGAAGATTTC
    AGGGATGGTC TTAAACTCAT GTTACTTCTG GAAGTCATTT CAGGTGAACG TTTGGCTAAG
    CCCGAAAGAG GCAAAATGCG AGTGCACAAA ATATCCAACG TGAACAAGGC CTTGGATTTC
    ATTGCCAGCA AAGGAGTCAA ACTAGTATCA ATTGGAGCAG AAGAAATTGT GGATGGCAAC
    GTTAAAATGA CTCTTGGAAT GATCTGGACC ATCATCCTTC GTTTTGCCAT TCAGGATATC
    TCAGTTGAAG AGACATCTGC TAAAGAAGGA CTTTTGTTAT GGTGTCAGAG GAAGACAGCT
    CCCTACAAAA ATGTAAACAT CCAGAACTTC CATATCAGTT GGAAAGATGG CCTTGGTTTC
    TGCGCTTTAA TTCACAGACA TCGTCCAGAG CTCATTGATT ACGGGAAGCT ACGAAAGGAT
    GATCCTCTCA CTAATCTAAA CACAGCCTTT GATGTAGCTG AAAAATATCT GGATATCCCC
    AAGATGCTGG ATGCAGAAGA CATTGTTGGA ACTGCCCGTC CTGACGAGAA AGCCATCATG
    ACCTATGTTT CTAGCTTCTA CCACGCCTTC TCAGGAGCCC AGAAGGCGGA GACAGCAGCA
    AATCGTATTT GCAAGGTTCT GGCAGTCAAC CAAGAGAATG AACAGCTCAT GGAAGACTAT
    GAAAAACTGG CCAGTGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC
    CGGGCACCAG AGAACACCAT GCAAGCCATG CAGCAGAAAC TGGAGGACTT CCGGGACTAC
    CGCCGCCTGC ATAAGCCCCC CAAAGTCCAA GAGAAGTGCC AACTTGAGAT CAATTTCAAC
    ACCTTGCAGA CCAAGCTACG GCTCAGCAAC AGGCCGGCCT TCATGCCTTC AGAAGGGAAA
    ATGGTTTCAG ATATAAACAA TGCCTGGGGT GGCCTAGAGC AGGCTGAGAA GGGCTATGAG
    GAGTGGTTGT TGAATGAGAT CCGGAGGCTA GAGAGGCTGG ATCACCTGGC AGAGAAGTTC
    CGGCAGAAAG CATCTATTCA CGAGTCCTGG ACAGATGGTA AGGAAGCGAT GCTGCAGCAG
    AAGGATTATG AAACTGCTAC CCTCTCGGAG ATAAAGGCCC TGCTGAAGAA ACACGAGGCC
    TTTGAGAGCG ACCTGGCTGC ACACCAGGAC CGCGTGGAAC AGATTGCTGC TATTGCACAA
    GAGCTGAATG AGCTGGACTA TTATGACTCT CCGAGTGTCA ATGCCAGGTG CCAGAAGATC
    TGTGATCAGT GGGATAATCT GGGTGCCCTA ACCCAGAAAC GTAGAGAAGC TTTAGAGAGG
    ACAGAGAAGC TACTGGAAAC AATTGACCAG TTGTACCTGG AATATGCCAA ACGAGCTGCC
    CCCTTCAATA ACTGGATGGA AGGGGCCATG GAGGACCTGC AGGACACGTT CATTGTTCAC
    ACCATTGAGG AGATCCAGGG ATTGACCACA GCCCACGAAC AGTTCAAAGC CACTTTGCCA
    GATGCCGACA AGGAGCGACA GGCCATCCTG GGAATCCACA ATGAAGTCTC AAAGATTGTC
    CAGACGTACC ACGTCAACAT GGCAGGAACG AACCCTTACA CTACAATCAC CCCACAGGAG
    ATCAATGGCA AATGGGAACA CGTGCGGCAG CTGGTTCCTA GAAGGGATCA AGCCCTAATG
    GAAGAACATG CTCGCCAGCA GCAAAATGAG CGACTTCGTA AGCAGTTTGG TGCACAAGCC
    AATGTCATTG GTCCCTGGAT TCAGACCAAG ATGGAGGAGA TTGGCCGCAT TTCAATAGAG
    ATGCACGGGA CTCTGGAGGA CCAGCTCAAC CACTTGCGGC AGTATGAGAA AAGCATTGTG
    AATTACAAAC CAAAGATCGA CCAGTTGGAA GGAGACCACC AACAGATCCA GGAGGCGCTT
    ATCTTTGACA ACAAGCACAC CAACTACACC ATGGAGCATA TTCGTGTGGG CTGGGAGCAG
    CTATTAACAA CAATTGCTAG AACCATTAAT GAAGTGGAAA ACCAGATTCT GACCCGTGAT
    GCCAAAGGAA TAAGCCAGGA ACAGATGAAT GAGTTCCGAG CCTCTTTCAA CCATTTTGAC
    AGGGACCACT CCGGCACACT TGGCCCTGAG GAATTCAAAG CCTGTCTCAT CAGCTTGGGT
    TACGATATTG GAAACGATGC ACAGAAGAAG ACTGGTATGA TGGATTGTGA AGATTTCCGA
    GCCTGCCTTA TCTCCATGGG TTACAATATG GGAGAGGCAG AGTTTGCCCG CATCATGAGC
    ATTGTCGATC CTAATCGCAT GGGTGTAGTG ACGTTCCAGG CCTTCATTGA CTTCATGTCC
    CGGGAAACAG CAGATACAGA TACTGCTGAC CAGGTTATGG CTTCTTTCAA GATCCTGGCT
    GGAGATAAGA ACTACATCAC TGTGGATGAG TTACGGCGGG AGCTGCCTCC TGATCAGGCT
    GAGTACTGCA TTGCTAGAAT GGCACCCTAC AATGGCCGTG ATGCTGTACC CGGTGCCCTG
    GACTACATGT CGTTCTCCAC GGCGCTCTAT GGCGAAAGTG ACCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015142743.1
    CDS69..2816
    Translation

    Target ORF information:

    RefSeq Version XM_015287257.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus actinin, alpha 1 (ACTN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287257.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGATCATC ACTACGACCC GCAGCAGACC AACGATTACA TGCAGCCGGA GGAGGACTGG 
    GACCGGGACC TGCTCCTCGA CCCGGCTTGG GAGAAGCAGC AGCGGAAGAC ATTTACAGCA
    TGGTGTAATT CTCACCTCCG CAAGGCTGGG ACACAGATTG AGAACATAGA GGAAGATTTC
    AGGGATGGTC TTAAACTCAT GTTACTTCTG GAAGTCATTT CAGGTGAACG TTTGGCTAAG
    CCCGAAAGAG GCAAAATGCG AGTGCACAAA ATATCCAACG TGAACAAGGC CTTGGATTTC
    ATTGCCAGCA AAGGAGTCAA ACTAGTATCA ATTGGAGCAG AAGAAATTGT GGATGGCAAC
    GTTAAAATGA CTCTTGGAAT GATCTGGACC ATCATCCTTC GTTTTGCCAT TCAGGATATC
    TCAGTTGAAG AGACATCTGC TAAAGAAGGA CTTTTGTTAT GGTGTCAGAG GAAGACAGCT
    CCCTACAAAA ATGTAAACAT CCAGAACTTC CATATCAGTT GGAAAGATGG CCTTGGTTTC
    TGCGCTTTAA TTCACAGACA TCGTCCAGAG CTCATTGATT ACGGGAAGCT ACGAAAGGAT
    GATCCTCTCA CTAATCTAAA CACAGCCTTT GATGTAGCTG AAAAATATCT GGATATCCCC
    AAGATGCTGG ATGCAGAAGA CATTGTTGGA ACTGCCCGTC CTGACGAGAA AGCCATCATG
    ACCTATGTTT CTAGCTTCTA CCACGCCTTC TCAGGAGCCC AGAAGGCGGA GACAGCAGCA
    AATCGTATTT GCAAGGTTCT GGCAGTCAAC CAAGAGAATG AACAGCTCAT GGAAGACTAT
    GAAAAACTGG CCAGTGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC
    CGGGCACCAG AGAACACCAT GCAAGCCATG CAGCAGAAAC TGGAGGACTT CCGGGACTAC
    CGCCGCCTGC ATAAGCCCCC CAAAGTCCAA GAGAAGTGCC AACTTGAGAT CAATTTCAAC
    ACCTTGCAGA CCAAGCTACG GCTCAGCAAC AGGCCGGCCT TCATGCCTTC AGAAGGGAAA
    ATGGTTTCAG ATATAAACAA TGCCTGGGGT GGCCTAGAGC AGGCTGAGAA GGGCTATGAG
    GAGTGGTTGT TGAATGAGAT CCGGAGGCTA GAGAGGCTGG ATCACCTGGC AGAGAAGTTC
    CGGCAGAAAG CATCTATTCA CGAGTCCTGG ACAGATGGTA AGGAAGCGAT GCTGCAGCAG
    AAGGATTATG AAACTGCTAC CCTCTCGGAG ATAAAGGCCC TGCTGAAGAA ACACGAGGCC
    TTTGAGAGCG ACCTGGCTGC ACACCAGGAC CGCGTGGAAC AGATTGCTGC TATTGCACAA
    GAGCTGAATG AGCTGGACTA TTATGACTCT CCGAGTGTCA ATGCCAGGTG CCAGAAGATC
    TGTGATCAGT GGGATAATCT GGGTGCCCTA ACCCAGAAAC GTAGAGAAGC TTTAGAGAGG
    ACAGAGAAGC TACTGGAAAC AATTGACCAG TTGTACCTGG AATATGCCAA ACGAGCTGCC
    CCCTTCAATA ACTGGATGGA AGGGGCCATG GAGGACCTGC AGGACACGTT CATTGTTCAC
    ACCATTGAGG AGATCCAGGG ATTGACCACA GCCCACGAAC AGTTCAAAGC CACTTTGCCA
    GATGCCGACA AGGAGCGACA GGCCATCCTG GGAATCCACA ATGAAGTCTC AAAGATTGTC
    CAGACGTACC ACGTCAACAT GGCAGGAACG AACCCTTACA CTACAATCAC CCCACAGGAG
    ATCAATGGCA AATGGGAACA CGTGCGGCAG CTGGTTCCTA GAAGGGATCA AGCCCTAATG
    GAAGAACATG CTCGCCAGCA GCAAAATGAG CGACTTCGTA AGCAGTTTGG TGCACAAGCC
    AATGTCATTG GTCCCTGGAT TCAGACCAAG ATGGAGGAGA TTGGCCGCAT TTCAATAGAG
    ATGCACGGGA CTCTGGAGGA CCAGCTCAAC CACTTGCGGC AGTATGAGAA AAGCATTGTG
    AATTACAAAC CAAAGATCGA CCAGTTGGAA GGAGACCACC AACAGATCCA GGAGGCGCTT
    ATCTTTGACA ACAAGCACAC CAACTACACC ATGGAGCATA TTCGTGTGGG CTGGGAGCAG
    CTATTAACAA CAATTGCTAG AACCATTAAT GAAGTGGAAA ACCAGATTCT GACCCGTGAT
    GCCAAAGGAA TAAGCCAGGA ACAGATGAAT GAGTTCCGAG CCTCTTTCAA CCATTTTGAC
    AGGGACCACT CCGGCACACT TGGCCCTGAG GAATTCAAAG CCTGTCTCAT CAGCTTGGGT
    TACGATATTG GAAACGATGC ACAGAAGAAG ACTGGTATGA TGGATTGTGA AGATTTCCGA
    GCCTGCCTTA TCTCCATGGG TTACAATATG GGAGAGGCAG AGTTTGCCCG CATCATGAGC
    ATTGTCGATC CTAATCGCAT GGGTGTAGTG ACGTTCCAGG CCTTCATTGA CTTCATGTCC
    CGGGAAACAG CAGATACAGA TACTGCTGAC CAGGTTATGG CTTCTTTCAA GATCCTGGCT
    GGAGATAAGA ACTACATCAC TGTGGATGAG TTACGGCGGG AGCTGCCTCC TGATCAGGCT
    GAGTACTGCA TTGCTAGAAT GGCACCCTAC AATGGCCGTG ATGCTGTACC CGGTGCCCTG
    GACTACATGT CGTTCTCCAC GGCGCTCTAT GGCGAAAGTG ACCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa36737
    Clone ID Related Accession (Same CDS sequence) XM_015287258.1 , XM_015287258.2
    Accession Version XM_015287258.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product alpha-actinin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGATCATC ACTACGACCC GCAGCAGACC AACGATTACA TGCAGCCGGA GGAGGACTGG 
    GACCGGGACC TGCTCCTCGA CCCGGCTTGG GAGAAGCAGC AGCGGAAGAC ATTTACAGCA
    TGGTGTAATT CTCACCTCCG CAAGGCTGGG ACACAGATTG AGAACATAGA GGAAGATTTC
    AGGGATGGTC TTAAACTCAT GTTACTTCTG GAAGTCATTT CAGGTGAACG TTTGGCTAAG
    CCCGAAAGAG GCAAAATGCG AGTGCACAAA ATATCCAACG TGAACAAGGC CTTGGATTTC
    ATTGCCAGCA AAGGAGTCAA ACTAGTATCA ATTGGAGCAG AAGAAATTGT GGATGGCAAC
    GTTAAAATGA CTCTTGGAAT GATCTGGACC ATCATCCTTC GTTTTGCCAT TCAGGATATC
    TCAGTTGAAG AGACATCTGC TAAAGAAGGA CTTTTGTTAT GGTGTCAGAG GAAGACAGCT
    CCCTACAAAA ATGTAAACAT CCAGAACTTC CATATCAGTT GGAAAGATGG CCTTGGTTTC
    TGCGCTTTAA TTCACAGACA TCGTCCAGAG CTCATTGATT ACGGGAAGCT ACGAAAGGAT
    GATCCTCTCA CTAATCTAAA CACAGCCTTT GATGTAGCTG AAAAATATCT GGATATCCCC
    AAGATGCTGG ATGCAGAAGA CATTGTTGGA ACTGCCCGTC CTGACGAGAA AGCCATCATG
    ACCTATGTTT CTAGCTTCTA CCACGCCTTC TCAGGAGCCC AGAAGGCGGA GACAGCAGCA
    AATCGTATTT GCAAGGTTCT GGCAGTCAAC CAAGAGAATG AACAGCTCAT GGAAGACTAT
    GAAAAACTGG CCAGTGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC
    CGGGCACCAG AGAACACCAT GCAAGCCATG CAGCAGAAAC TGGAGGACTT CCGGGACTAC
    CGCCGCCTGC ATAAGCCCCC CAAAGTCCAA GAGAAGTGCC AACTTGAGAT CAATTTCAAC
    ACCTTGCAGA CCAAGCTACG GCTCAGCAAC AGGCCGGCCT TCATGCCTTC AGAAGGGAAA
    ATGGTTTCAG ATATAAACAA TGCCTGGGGT GGCCTAGAGC AGGCTGAGAA GGGCTATGAG
    GAGTGGTTGT TGAATGAGAT CCGGAGGCTA GAGAGGCTGG ATCACCTGGC AGAGAAGTTC
    CGGCAGAAAG CATCTATTCA CGAGTCCTGG ACAGATGGTA AGGAAGCGAT GCTGCAGCAG
    AAGGATTATG AAACTGCTAC CCTCTCGGAG ATAAAGGCCC TGCTGAAGAA ACACGAGGCC
    TTTGAGAGCG ACCTGGCTGC ACACCAGGAC CGCGTGGAAC AGATTGCTGC TATTGCACAA
    GAGCTGAATG AGCTGGACTA TTATGACTCT CCGAGTGTCA ATGCCAGGTG CCAGAAGATC
    TGTGATCAGT GGGATAATCT GGGTGCCCTA ACCCAGAAAC GTAGAGAAGC TTTAGAGAGG
    ACAGAGAAGC TACTGGAAAC AATTGACCAG TTGTACCTGG AATATGCCAA ACGAGCTGCC
    CCCTTCAATA ACTGGATGGA AGGGGCCATG GAGGACCTGC AGGACACGTT CATTGTTCAC
    ACCATTGAGG AGATCCAGGG ATTGACCACA GCCCACGAAC AGTTCAAAGC CACTTTGCCA
    GATGCCGACA AGGAGCGACA GGCCATCCTG GGAATCCACA ATGAAGTCTC AAAGATTGTC
    CAGACGTACC ACGTCAACAT GGCAGGAACG AACCCTTACA CTACAATCAC CCCACAGGAG
    ATCAATGGCA AATGGGAACA CGTGCGGCAG CTGGTTCCTA GAAGGGATCA AGCCCTAATG
    GAAGAACATG CTCGCCAGCA GCAAAATGAG CGACTTCGTA AGCAGTTTGG TGCACAAGCC
    AATGTCATTG GTCCCTGGAT TCAGACCAAG ATGGAGGAGA TTGGCCGCAT TTCAATAGAG
    ATGCACGGGA CTCTGGAGGA CCAGCTCAAC CACTTGCGGC AGTATGAGAA AAGCATTGTG
    AATTACAAAC CAAAGATCGA CCAGTTGGAA GGAGACCACC AACAGATCCA GGAGGCGCTT
    ATCTTTGACA ACAAGCACAC CAACTACACC ATGGAGCATA TTCGTGTGGG CTGGGAGCAG
    CTATTAACAA CAATTGCTAG AACCATTAAT GAAGTGGAAA ACCAGATTCT GACCCGTGAT
    GCCAAAGGAA TAAGCCAGGA ACAGATGAAT GAGTTCCGAG CCTCTTTCAA CCATTTTGAC
    AGGGACCACT CCGGCACACT TGGCCCTGAG GAATTCAAAG CCTGTCTCAT CAGCTTGGGT
    TACGATATTG GAAACGATGC ACAGGGAGAG GCAGAGTTTG CCCGCATCAT GAGCATTGTC
    GATCCTAATC GCATGGGTGT AGTGACGTTC CAGGCCTTCA TTGACTTCAT GTCCCGGGAA
    ACAGCAGATA CAGATACTGC TGACCAGGTT ATGGCTTCTT TCAAGATCCT GGCTGGAGAT
    AAGAACTACA TCACTGTGGA TGAGTTACGG CGGGAGCTGC CTCCTGATCA GGCTGAGTAC
    TGCATTGCTA GAATGGCACC CTACAATGGC CGTGATGCTG TACCCGGTGC CCTGGACTAC
    ATGTCGTTCT CCACGGCGCT CTATGGCGAA AGTGACCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015142744.1
    CDS4..2685
    Translation

    Target ORF information:

    RefSeq Version XM_015287258.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus actinin, alpha 1 (ACTN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287258.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGATCATC ACTACGACCC GCAGCAGACC AACGATTACA TGCAGCCGGA GGAGGACTGG 
    GACCGGGACC TGCTCCTCGA CCCGGCTTGG GAGAAGCAGC AGCGGAAGAC ATTTACAGCA
    TGGTGTAATT CTCACCTCCG CAAGGCTGGG ACACAGATTG AGAACATAGA GGAAGATTTC
    AGGGATGGTC TTAAACTCAT GTTACTTCTG GAAGTCATTT CAGGTGAACG TTTGGCTAAG
    CCCGAAAGAG GCAAAATGCG AGTGCACAAA ATATCCAACG TGAACAAGGC CTTGGATTTC
    ATTGCCAGCA AAGGAGTCAA ACTAGTATCA ATTGGAGCAG AAGAAATTGT GGATGGCAAC
    GTTAAAATGA CTCTTGGAAT GATCTGGACC ATCATCCTTC GTTTTGCCAT TCAGGATATC
    TCAGTTGAAG AGACATCTGC TAAAGAAGGA CTTTTGTTAT GGTGTCAGAG GAAGACAGCT
    CCCTACAAAA ATGTAAACAT CCAGAACTTC CATATCAGTT GGAAAGATGG CCTTGGTTTC
    TGCGCTTTAA TTCACAGACA TCGTCCAGAG CTCATTGATT ACGGGAAGCT ACGAAAGGAT
    GATCCTCTCA CTAATCTAAA CACAGCCTTT GATGTAGCTG AAAAATATCT GGATATCCCC
    AAGATGCTGG ATGCAGAAGA CATTGTTGGA ACTGCCCGTC CTGACGAGAA AGCCATCATG
    ACCTATGTTT CTAGCTTCTA CCACGCCTTC TCAGGAGCCC AGAAGGCGGA GACAGCAGCA
    AATCGTATTT GCAAGGTTCT GGCAGTCAAC CAAGAGAATG AACAGCTCAT GGAAGACTAT
    GAAAAACTGG CCAGTGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC
    CGGGCACCAG AGAACACCAT GCAAGCCATG CAGCAGAAAC TGGAGGACTT CCGGGACTAC
    CGCCGCCTGC ATAAGCCCCC CAAAGTCCAA GAGAAGTGCC AACTTGAGAT CAATTTCAAC
    ACCTTGCAGA CCAAGCTACG GCTCAGCAAC AGGCCGGCCT TCATGCCTTC AGAAGGGAAA
    ATGGTTTCAG ATATAAACAA TGCCTGGGGT GGCCTAGAGC AGGCTGAGAA GGGCTATGAG
    GAGTGGTTGT TGAATGAGAT CCGGAGGCTA GAGAGGCTGG ATCACCTGGC AGAGAAGTTC
    CGGCAGAAAG CATCTATTCA CGAGTCCTGG ACAGATGGTA AGGAAGCGAT GCTGCAGCAG
    AAGGATTATG AAACTGCTAC CCTCTCGGAG ATAAAGGCCC TGCTGAAGAA ACACGAGGCC
    TTTGAGAGCG ACCTGGCTGC ACACCAGGAC CGCGTGGAAC AGATTGCTGC TATTGCACAA
    GAGCTGAATG AGCTGGACTA TTATGACTCT CCGAGTGTCA ATGCCAGGTG CCAGAAGATC
    TGTGATCAGT GGGATAATCT GGGTGCCCTA ACCCAGAAAC GTAGAGAAGC TTTAGAGAGG
    ACAGAGAAGC TACTGGAAAC AATTGACCAG TTGTACCTGG AATATGCCAA ACGAGCTGCC
    CCCTTCAATA ACTGGATGGA AGGGGCCATG GAGGACCTGC AGGACACGTT CATTGTTCAC
    ACCATTGAGG AGATCCAGGG ATTGACCACA GCCCACGAAC AGTTCAAAGC CACTTTGCCA
    GATGCCGACA AGGAGCGACA GGCCATCCTG GGAATCCACA ATGAAGTCTC AAAGATTGTC
    CAGACGTACC ACGTCAACAT GGCAGGAACG AACCCTTACA CTACAATCAC CCCACAGGAG
    ATCAATGGCA AATGGGAACA CGTGCGGCAG CTGGTTCCTA GAAGGGATCA AGCCCTAATG
    GAAGAACATG CTCGCCAGCA GCAAAATGAG CGACTTCGTA AGCAGTTTGG TGCACAAGCC
    AATGTCATTG GTCCCTGGAT TCAGACCAAG ATGGAGGAGA TTGGCCGCAT TTCAATAGAG
    ATGCACGGGA CTCTGGAGGA CCAGCTCAAC CACTTGCGGC AGTATGAGAA AAGCATTGTG
    AATTACAAAC CAAAGATCGA CCAGTTGGAA GGAGACCACC AACAGATCCA GGAGGCGCTT
    ATCTTTGACA ACAAGCACAC CAACTACACC ATGGAGCATA TTCGTGTGGG CTGGGAGCAG
    CTATTAACAA CAATTGCTAG AACCATTAAT GAAGTGGAAA ACCAGATTCT GACCCGTGAT
    GCCAAAGGAA TAAGCCAGGA ACAGATGAAT GAGTTCCGAG CCTCTTTCAA CCATTTTGAC
    AGGGACCACT CCGGCACACT TGGCCCTGAG GAATTCAAAG CCTGTCTCAT CAGCTTGGGT
    TACGATATTG GAAACGATGC ACAGGGAGAG GCAGAGTTTG CCCGCATCAT GAGCATTGTC
    GATCCTAATC GCATGGGTGT AGTGACGTTC CAGGCCTTCA TTGACTTCAT GTCCCGGGAA
    ACAGCAGATA CAGATACTGC TGACCAGGTT ATGGCTTCTT TCAAGATCCT GGCTGGAGAT
    AAGAACTACA TCACTGTGGA TGAGTTACGG CGGGAGCTGC CTCCTGATCA GGCTGAGTAC
    TGCATTGCTA GAATGGCACC CTACAATGGC CGTGATGCTG TACCCGGTGC CCTGGACTAC
    ATGTCGTTCT CCACGGCGCT CTATGGCGAA AGTGACCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa36737
    Clone ID Related Accession (Same CDS sequence) XM_015287258.1 , XM_015287258.2
    Accession Version XM_015287258.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product alpha-actinin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287258.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGATCATC ACTACGACCC GCAGCAGACC AACGATTACA TGCAGCCGGA GGAGGACTGG 
    GACCGGGACC TGCTCCTCGA CCCGGCTTGG GAGAAGCAGC AGCGGAAGAC ATTTACAGCA
    TGGTGTAATT CTCACCTCCG CAAGGCTGGG ACACAGATTG AGAACATAGA GGAAGATTTC
    AGGGATGGTC TTAAACTCAT GTTACTTCTG GAAGTCATTT CAGGTGAACG TTTGGCTAAG
    CCCGAAAGAG GCAAAATGCG AGTGCACAAA ATATCCAACG TGAACAAGGC CTTGGATTTC
    ATTGCCAGCA AAGGAGTCAA ACTAGTATCA ATTGGAGCAG AAGAAATTGT GGATGGCAAC
    GTTAAAATGA CTCTTGGAAT GATCTGGACC ATCATCCTTC GTTTTGCCAT TCAGGATATC
    TCAGTTGAAG AGACATCTGC TAAAGAAGGA CTTTTGTTAT GGTGTCAGAG GAAGACAGCT
    CCCTACAAAA ATGTAAACAT CCAGAACTTC CATATCAGTT GGAAAGATGG CCTTGGTTTC
    TGCGCTTTAA TTCACAGACA TCGTCCAGAG CTCATTGATT ACGGGAAGCT ACGAAAGGAT
    GATCCTCTCA CTAATCTAAA CACAGCCTTT GATGTAGCTG AAAAATATCT GGATATCCCC
    AAGATGCTGG ATGCAGAAGA CATTGTTGGA ACTGCCCGTC CTGACGAGAA AGCCATCATG
    ACCTATGTTT CTAGCTTCTA CCACGCCTTC TCAGGAGCCC AGAAGGCGGA GACAGCAGCA
    AATCGTATTT GCAAGGTTCT GGCAGTCAAC CAAGAGAATG AACAGCTCAT GGAAGACTAT
    GAAAAACTGG CCAGTGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC
    CGGGCACCAG AGAACACCAT GCAAGCCATG CAGCAGAAAC TGGAGGACTT CCGGGACTAC
    CGCCGCCTGC ATAAGCCCCC CAAAGTCCAA GAGAAGTGCC AACTTGAGAT CAATTTCAAC
    ACCTTGCAGA CCAAGCTACG GCTCAGCAAC AGGCCGGCCT TCATGCCTTC AGAAGGGAAA
    ATGGTTTCAG ATATAAACAA TGCCTGGGGT GGCCTAGAGC AGGCTGAGAA GGGCTATGAG
    GAGTGGTTGT TGAATGAGAT CCGGAGGCTA GAGAGGCTGG ATCACCTGGC AGAGAAGTTC
    CGGCAGAAAG CATCTATTCA CGAGTCCTGG ACAGATGGTA AGGAAGCGAT GCTGCAGCAG
    AAGGATTATG AAACTGCTAC CCTCTCGGAG ATAAAGGCCC TGCTGAAGAA ACACGAGGCC
    TTTGAGAGCG ACCTGGCTGC ACACCAGGAC CGCGTGGAAC AGATTGCTGC TATTGCACAA
    GAGCTGAATG AGCTGGACTA TTATGACTCT CCGAGTGTCA ATGCCAGGTG CCAGAAGATC
    TGTGATCAGT GGGATAATCT GGGTGCCCTA ACCCAGAAAC GTAGAGAAGC TTTAGAGAGG
    ACAGAGAAGC TACTGGAAAC AATTGACCAG TTGTACCTGG AATATGCCAA ACGAGCTGCC
    CCCTTCAATA ACTGGATGGA AGGGGCCATG GAGGACCTGC AGGACACGTT CATTGTTCAC
    ACCATTGAGG AGATCCAGGG ATTGACCACA GCCCACGAAC AGTTCAAAGC CACTTTGCCA
    GATGCCGACA AGGAGCGACA GGCCATCCTG GGAATCCACA ATGAAGTCTC AAAGATTGTC
    CAGACGTACC ACGTCAACAT GGCAGGAACG AACCCTTACA CTACAATCAC CCCACAGGAG
    ATCAATGGCA AATGGGAACA CGTGCGGCAG CTGGTTCCTA GAAGGGATCA AGCCCTAATG
    GAAGAACATG CTCGCCAGCA GCAAAATGAG CGACTTCGTA AGCAGTTTGG TGCACAAGCC
    AATGTCATTG GTCCCTGGAT TCAGACCAAG ATGGAGGAGA TTGGCCGCAT TTCAATAGAG
    ATGCACGGGA CTCTGGAGGA CCAGCTCAAC CACTTGCGGC AGTATGAGAA AAGCATTGTG
    AATTACAAAC CAAAGATCGA CCAGTTGGAA GGAGACCACC AACAGATCCA GGAGGCGCTT
    ATCTTTGACA ACAAGCACAC CAACTACACC ATGGAGCATA TTCGTGTGGG CTGGGAGCAG
    CTATTAACAA CAATTGCTAG AACCATTAAT GAAGTGGAAA ACCAGATTCT GACCCGTGAT
    GCCAAAGGAA TAAGCCAGGA ACAGATGAAT GAGTTCCGAG CCTCTTTCAA CCATTTTGAC
    AGGGACCACT CCGGCACACT TGGCCCTGAG GAATTCAAAG CCTGTCTCAT CAGCTTGGGT
    TACGATATTG GAAACGATGC ACAGGGAGAG GCAGAGTTTG CCCGCATCAT GAGCATTGTC
    GATCCTAATC GCATGGGTGT AGTGACGTTC CAGGCCTTCA TTGACTTCAT GTCCCGGGAA
    ACAGCAGATA CAGATACTGC TGACCAGGTT ATGGCTTCTT TCAAGATCCT GGCTGGAGAT
    AAGAACTACA TCACTGTGGA TGAGTTACGG CGGGAGCTGC CTCCTGATCA GGCTGAGTAC
    TGCATTGCTA GAATGGCACC CTACAATGGC CGTGATGCTG TACCCGGTGC CCTGGACTAC
    ATGTCGTTCT CCACGGCGCT CTATGGCGAA AGTGACCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015142744.1
    CDS98..2779
    Translation

    Target ORF information:

    RefSeq Version XM_015287258.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus actinin, alpha 1 (ACTN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287258.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGATCATC ACTACGACCC GCAGCAGACC AACGATTACA TGCAGCCGGA GGAGGACTGG 
    GACCGGGACC TGCTCCTCGA CCCGGCTTGG GAGAAGCAGC AGCGGAAGAC ATTTACAGCA
    TGGTGTAATT CTCACCTCCG CAAGGCTGGG ACACAGATTG AGAACATAGA GGAAGATTTC
    AGGGATGGTC TTAAACTCAT GTTACTTCTG GAAGTCATTT CAGGTGAACG TTTGGCTAAG
    CCCGAAAGAG GCAAAATGCG AGTGCACAAA ATATCCAACG TGAACAAGGC CTTGGATTTC
    ATTGCCAGCA AAGGAGTCAA ACTAGTATCA ATTGGAGCAG AAGAAATTGT GGATGGCAAC
    GTTAAAATGA CTCTTGGAAT GATCTGGACC ATCATCCTTC GTTTTGCCAT TCAGGATATC
    TCAGTTGAAG AGACATCTGC TAAAGAAGGA CTTTTGTTAT GGTGTCAGAG GAAGACAGCT
    CCCTACAAAA ATGTAAACAT CCAGAACTTC CATATCAGTT GGAAAGATGG CCTTGGTTTC
    TGCGCTTTAA TTCACAGACA TCGTCCAGAG CTCATTGATT ACGGGAAGCT ACGAAAGGAT
    GATCCTCTCA CTAATCTAAA CACAGCCTTT GATGTAGCTG AAAAATATCT GGATATCCCC
    AAGATGCTGG ATGCAGAAGA CATTGTTGGA ACTGCCCGTC CTGACGAGAA AGCCATCATG
    ACCTATGTTT CTAGCTTCTA CCACGCCTTC TCAGGAGCCC AGAAGGCGGA GACAGCAGCA
    AATCGTATTT GCAAGGTTCT GGCAGTCAAC CAAGAGAATG AACAGCTCAT GGAAGACTAT
    GAAAAACTGG CCAGTGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC
    CGGGCACCAG AGAACACCAT GCAAGCCATG CAGCAGAAAC TGGAGGACTT CCGGGACTAC
    CGCCGCCTGC ATAAGCCCCC CAAAGTCCAA GAGAAGTGCC AACTTGAGAT CAATTTCAAC
    ACCTTGCAGA CCAAGCTACG GCTCAGCAAC AGGCCGGCCT TCATGCCTTC AGAAGGGAAA
    ATGGTTTCAG ATATAAACAA TGCCTGGGGT GGCCTAGAGC AGGCTGAGAA GGGCTATGAG
    GAGTGGTTGT TGAATGAGAT CCGGAGGCTA GAGAGGCTGG ATCACCTGGC AGAGAAGTTC
    CGGCAGAAAG CATCTATTCA CGAGTCCTGG ACAGATGGTA AGGAAGCGAT GCTGCAGCAG
    AAGGATTATG AAACTGCTAC CCTCTCGGAG ATAAAGGCCC TGCTGAAGAA ACACGAGGCC
    TTTGAGAGCG ACCTGGCTGC ACACCAGGAC CGCGTGGAAC AGATTGCTGC TATTGCACAA
    GAGCTGAATG AGCTGGACTA TTATGACTCT CCGAGTGTCA ATGCCAGGTG CCAGAAGATC
    TGTGATCAGT GGGATAATCT GGGTGCCCTA ACCCAGAAAC GTAGAGAAGC TTTAGAGAGG
    ACAGAGAAGC TACTGGAAAC AATTGACCAG TTGTACCTGG AATATGCCAA ACGAGCTGCC
    CCCTTCAATA ACTGGATGGA AGGGGCCATG GAGGACCTGC AGGACACGTT CATTGTTCAC
    ACCATTGAGG AGATCCAGGG ATTGACCACA GCCCACGAAC AGTTCAAAGC CACTTTGCCA
    GATGCCGACA AGGAGCGACA GGCCATCCTG GGAATCCACA ATGAAGTCTC AAAGATTGTC
    CAGACGTACC ACGTCAACAT GGCAGGAACG AACCCTTACA CTACAATCAC CCCACAGGAG
    ATCAATGGCA AATGGGAACA CGTGCGGCAG CTGGTTCCTA GAAGGGATCA AGCCCTAATG
    GAAGAACATG CTCGCCAGCA GCAAAATGAG CGACTTCGTA AGCAGTTTGG TGCACAAGCC
    AATGTCATTG GTCCCTGGAT TCAGACCAAG ATGGAGGAGA TTGGCCGCAT TTCAATAGAG
    ATGCACGGGA CTCTGGAGGA CCAGCTCAAC CACTTGCGGC AGTATGAGAA AAGCATTGTG
    AATTACAAAC CAAAGATCGA CCAGTTGGAA GGAGACCACC AACAGATCCA GGAGGCGCTT
    ATCTTTGACA ACAAGCACAC CAACTACACC ATGGAGCATA TTCGTGTGGG CTGGGAGCAG
    CTATTAACAA CAATTGCTAG AACCATTAAT GAAGTGGAAA ACCAGATTCT GACCCGTGAT
    GCCAAAGGAA TAAGCCAGGA ACAGATGAAT GAGTTCCGAG CCTCTTTCAA CCATTTTGAC
    AGGGACCACT CCGGCACACT TGGCCCTGAG GAATTCAAAG CCTGTCTCAT CAGCTTGGGT
    TACGATATTG GAAACGATGC ACAGGGAGAG GCAGAGTTTG CCCGCATCAT GAGCATTGTC
    GATCCTAATC GCATGGGTGT AGTGACGTTC CAGGCCTTCA TTGACTTCAT GTCCCGGGAA
    ACAGCAGATA CAGATACTGC TGACCAGGTT ATGGCTTCTT TCAAGATCCT GGCTGGAGAT
    AAGAACTACA TCACTGTGGA TGAGTTACGG CGGGAGCTGC CTCCTGATCA GGCTGAGTAC
    TGCATTGCTA GAATGGCACC CTACAATGGC CGTGATGCTG TACCCGGTGC CCTGGACTAC
    ATGTCGTTCT CCACGGCGCT CTATGGCGAA AGTGACCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa36738
    Clone ID Related Accession (Same CDS sequence) XM_015287259.2 , XM_015287259.1
    Accession Version XM_015287259.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2667bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product alpha-actinin-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287259.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGATCATC ACTACGACCC GCAGCAGACC AACGATTACA TGCAGCCGGA GGAGGACTGG 
    GACCGGGACC TGCTCCTCGA CCCGGCTTGG GAGAAGCAGC AGCGGAAGAC ATTTACAGCA
    TGGTGTAATT CTCACCTCCG CAAGGCTGGG ACACAGATTG AGAACATAGA GGAAGATTTC
    AGGGATGGTC TTAAACTCAT GTTACTTCTG GAAGTCATTT CAGGTGAACG TTTGGCTAAG
    CCCGAAAGAG GCAAAATGCG AGTGCACAAA ATATCCAACG TGAACAAGGC CTTGGATTTC
    ATTGCCAGCA AAGGAGTCAA ACTAGTATCA ATTGGAGCAG AAGAAATTGT GGATGGCAAC
    GTTAAAATGA CTCTTGGAAT GATCTGGACC ATCATCCTTC GTTTTGCCAT TCAGGATATC
    TCAGTTGAAG AGACATCTGC TAAAGAAGGA CTTTTGTTAT GGTGTCAGAG GAAGACAGCT
    CCCTACAAAA ATGTAAACAT CCAGAACTTC CATATCAGTT GGAAAGATGG CCTTGGTTTC
    TGCGCTTTAA TTCACAGACA TCGTCCAGAG CTCATTGATT ACGGGAAGCT ACGAAAGGAT
    GATCCTCTCA CTAATCTAAA CACAGCCTTT GATGTAGCTG AAAAATATCT GGATATCCCC
    AAGATGCTGG ATGCAGAAGA TATTATAGGC ACGCTAAGGC CGGATGAGAA GGCCATAATG
    ACCTATGTTT CATGTTACTA CCACGCCTTC TCAGGGGCAC AGAAGGCGGA GACAGCAGCA
    AATCGTATTT GCAAGGTTCT GGCAGTCAAC CAAGAGAATG AACAGCTCAT GGAAGACTAT
    GAAAAACTGG CCAGTGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC
    CGGGCACCAG AGAACACCAT GCAAGCCATG CAGCAGAAAC TGGAGGACTT CCGGGACTAC
    CGCCGCCTGC ATAAGCCCCC CAAAGTCCAA GAGAAGTGCC AACTTGAGAT CAATTTCAAC
    ACCTTGCAGA CCAAGCTACG GCTCAGCAAC AGGCCGGCCT TCATGCCTTC AGAAGGGAAA
    ATGGTTTCAG ATATAAACAA TGCCTGGGGT GGCCTAGAGC AGGCTGAGAA GGGCTATGAG
    GAGTGGTTGT TGAATGAGAT CCGGAGGCTA GAGAGGCTGG ATCACCTGGC AGAGAAGTTC
    CGGCAGAAAG CATCTATTCA CGAGTCCTGG ACAGATGGTA AGGAAGCGAT GCTGCAGCAG
    AAGGATTATG AAACTGCTAC CCTCTCGGAG ATAAAGGCCC TGCTGAAGAA ACACGAGGCC
    TTTGAGAGCG ACCTGGCTGC ACACCAGGAC CGCGTGGAAC AGATTGCTGC TATTGCACAA
    GAGCTGAATG AGCTGGACTA TTATGACTCT CCGAGTGTCA ATGCCAGGTG CCAGAAGATC
    TGTGATCAGT GGGATAATCT GGGTGCCCTA ACCCAGAAAC GTAGAGAAGC TTTAGAGAGG
    ACAGAGAAGC TACTGGAAAC AATTGACCAG TTGTACCTGG AATATGCCAA ACGAGCTGCC
    CCCTTCAATA ACTGGATGGA AGGGGCCATG GAGGACCTGC AGGACACGTT CATTGTTCAC
    ACCATTGAGG AGATCCAGGG ATTGACCACA GCCCACGAAC AGTTCAAAGC CACTTTGCCA
    GATGCCGACA AGGAGCGACA GGCCATCCTG GGAATCCACA ATGAAGTCTC AAAGATTGTC
    CAGACGTACC ACGTCAACAT GGCAGGAACG AACCCTTACA CTACAATCAC CCCACAGGAG
    ATCAATGGCA AATGGGAACA CGTGCGGCAG CTGGTTCCTA GAAGGGATCA AGCCCTAATG
    GAAGAACATG CTCGCCAGCA GCAAAATGAG CGACTTCGTA AGCAGTTTGG TGCACAAGCC
    AATGTCATTG GTCCCTGGAT TCAGACCAAG ATGGAGGAGA TTGGCCGCAT TTCAATAGAG
    ATGCACGGGA CTCTGGAGGA CCAGCTCAAC CACTTGCGGC AGTATGAGAA AAGCATTGTG
    AATTACAAAC CAAAGATCGA CCAGTTGGAA GGAGACCACC AACAGATCCA GGAGGCGCTT
    ATCTTTGACA ACAAGCACAC CAACTACACC ATGGAGCATA TTCGTGTGGG CTGGGAGCAG
    CTATTAACAA CAATTGCTAG AACCATTAAT GAAGTGGAAA ACCAGATTCT GACCCGTGAT
    GCCAAAGGAA TAAGCCAGGA ACAGATGAAT GAGTTCCGAG CCTCTTTCAA CCATTTTGAC
    AGGAAGAAGA CTGGTATGAT GGATTGTGAA GATTTCCGAG CCTGCCTTAT CTCCATGGGT
    TACAATATGG GAGAGGCAGA GTTTGCCCGC ATCATGAGCA TTGTCGATCC TAATCGCATG
    GGTGTAGTGA CGTTCCAGGC CTTCATTGAC TTCATGTCCC GGGAAACAGC AGATACAGAT
    ACTGCTGACC AGGTTATGGC TTCTTTCAAG ATCCTGGCTG GAGATAAGAA CTACATCACT
    GTGGATGAGT TACGGCGGGA GCTGCCTCCT GATCAGGCTG AGTACTGCAT TGCTAGAATG
    GCACCCTACA ATGGCCGTGA TGCTGTACCC GGTGCCCTGG ACTACATGTC GTTCTCCACG
    GCGCTCTATG GCGAAAGTGA CCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015142745.1
    CDS69..2735
    Translation

    Target ORF information:

    RefSeq Version XM_015287259.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus actinin, alpha 1 (ACTN1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287259.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGATCATC ACTACGACCC GCAGCAGACC AACGATTACA TGCAGCCGGA GGAGGACTGG 
    GACCGGGACC TGCTCCTCGA CCCGGCTTGG GAGAAGCAGC AGCGGAAGAC ATTTACAGCA
    TGGTGTAATT CTCACCTCCG CAAGGCTGGG ACACAGATTG AGAACATAGA GGAAGATTTC
    AGGGATGGTC TTAAACTCAT GTTACTTCTG GAAGTCATTT CAGGTGAACG TTTGGCTAAG
    CCCGAAAGAG GCAAAATGCG AGTGCACAAA ATATCCAACG TGAACAAGGC CTTGGATTTC
    ATTGCCAGCA AAGGAGTCAA ACTAGTATCA ATTGGAGCAG AAGAAATTGT GGATGGCAAC
    GTTAAAATGA CTCTTGGAAT GATCTGGACC ATCATCCTTC GTTTTGCCAT TCAGGATATC
    TCAGTTGAAG AGACATCTGC TAAAGAAGGA CTTTTGTTAT GGTGTCAGAG GAAGACAGCT
    CCCTACAAAA ATGTAAACAT CCAGAACTTC CATATCAGTT GGAAAGATGG CCTTGGTTTC
    TGCGCTTTAA TTCACAGACA TCGTCCAGAG CTCATTGATT ACGGGAAGCT ACGAAAGGAT
    GATCCTCTCA CTAATCTAAA CACAGCCTTT GATGTAGCTG AAAAATATCT GGATATCCCC
    AAGATGCTGG ATGCAGAAGA TATTATAGGC ACGCTAAGGC CGGATGAGAA GGCCATAATG
    ACCTATGTTT CATGTTACTA CCACGCCTTC TCAGGGGCAC AGAAGGCGGA GACAGCAGCA
    AATCGTATTT GCAAGGTTCT GGCAGTCAAC CAAGAGAATG AACAGCTCAT GGAAGACTAT
    GAAAAACTGG CCAGTGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC
    CGGGCACCAG AGAACACCAT GCAAGCCATG CAGCAGAAAC TGGAGGACTT CCGGGACTAC
    CGCCGCCTGC ATAAGCCCCC CAAAGTCCAA GAGAAGTGCC AACTTGAGAT CAATTTCAAC
    ACCTTGCAGA CCAAGCTACG GCTCAGCAAC AGGCCGGCCT TCATGCCTTC AGAAGGGAAA
    ATGGTTTCAG ATATAAACAA TGCCTGGGGT GGCCTAGAGC AGGCTGAGAA GGGCTATGAG
    GAGTGGTTGT TGAATGAGAT CCGGAGGCTA GAGAGGCTGG ATCACCTGGC AGAGAAGTTC
    CGGCAGAAAG CATCTATTCA CGAGTCCTGG ACAGATGGTA AGGAAGCGAT GCTGCAGCAG
    AAGGATTATG AAACTGCTAC CCTCTCGGAG ATAAAGGCCC TGCTGAAGAA ACACGAGGCC
    TTTGAGAGCG ACCTGGCTGC ACACCAGGAC CGCGTGGAAC AGATTGCTGC TATTGCACAA
    GAGCTGAATG AGCTGGACTA TTATGACTCT CCGAGTGTCA ATGCCAGGTG CCAGAAGATC
    TGTGATCAGT GGGATAATCT GGGTGCCCTA ACCCAGAAAC GTAGAGAAGC TTTAGAGAGG
    ACAGAGAAGC TACTGGAAAC AATTGACCAG TTGTACCTGG AATATGCCAA ACGAGCTGCC
    CCCTTCAATA ACTGGATGGA AGGGGCCATG GAGGACCTGC AGGACACGTT CATTGTTCAC
    ACCATTGAGG AGATCCAGGG ATTGACCACA GCCCACGAAC AGTTCAAAGC CACTTTGCCA
    GATGCCGACA AGGAGCGACA GGCCATCCTG GGAATCCACA ATGAAGTCTC AAAGATTGTC
    CAGACGTACC ACGTCAACAT GGCAGGAACG AACCCTTACA CTACAATCAC CCCACAGGAG
    ATCAATGGCA AATGGGAACA CGTGCGGCAG CTGGTTCCTA GAAGGGATCA AGCCCTAATG
    GAAGAACATG CTCGCCAGCA GCAAAATGAG CGACTTCGTA AGCAGTTTGG TGCACAAGCC
    AATGTCATTG GTCCCTGGAT TCAGACCAAG ATGGAGGAGA TTGGCCGCAT TTCAATAGAG
    ATGCACGGGA CTCTGGAGGA CCAGCTCAAC CACTTGCGGC AGTATGAGAA AAGCATTGTG
    AATTACAAAC CAAAGATCGA CCAGTTGGAA GGAGACCACC AACAGATCCA GGAGGCGCTT
    ATCTTTGACA ACAAGCACAC CAACTACACC ATGGAGCATA TTCGTGTGGG CTGGGAGCAG
    CTATTAACAA CAATTGCTAG AACCATTAAT GAAGTGGAAA ACCAGATTCT GACCCGTGAT
    GCCAAAGGAA TAAGCCAGGA ACAGATGAAT GAGTTCCGAG CCTCTTTCAA CCATTTTGAC
    AGGAAGAAGA CTGGTATGAT GGATTGTGAA GATTTCCGAG CCTGCCTTAT CTCCATGGGT
    TACAATATGG GAGAGGCAGA GTTTGCCCGC ATCATGAGCA TTGTCGATCC TAATCGCATG
    GGTGTAGTGA CGTTCCAGGC CTTCATTGAC TTCATGTCCC GGGAAACAGC AGATACAGAT
    ACTGCTGACC AGGTTATGGC TTCTTTCAAG ATCCTGGCTG GAGATAAGAA CTACATCACT
    GTGGATGAGT TACGGCGGGA GCTGCCTCCT GATCAGGCTG AGTACTGCAT TGCTAGAATG
    GCACCCTACA ATGGCCGTGA TGCTGTACCC GGTGCCCTGG ACTACATGTC GTTCTCCACG
    GCGCTCTATG GCGAAAGTGA CCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa36738
    Clone ID Related Accession (Same CDS sequence) XM_015287259.2 , XM_015287259.1
    Accession Version XM_015287259.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2667bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product alpha-actinin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGATCATC ACTACGACCC GCAGCAGACC AACGATTACA TGCAGCCGGA GGAGGACTGG 
    GACCGGGACC TGCTCCTCGA CCCGGCTTGG GAGAAGCAGC AGCGGAAGAC ATTTACAGCA
    TGGTGTAATT CTCACCTCCG CAAGGCTGGG ACACAGATTG AGAACATAGA GGAAGATTTC
    AGGGATGGTC TTAAACTCAT GTTACTTCTG GAAGTCATTT CAGGTGAACG TTTGGCTAAG
    CCCGAAAGAG GCAAAATGCG AGTGCACAAA ATATCCAACG TGAACAAGGC CTTGGATTTC
    ATTGCCAGCA AAGGAGTCAA ACTAGTATCA ATTGGAGCAG AAGAAATTGT GGATGGCAAC
    GTTAAAATGA CTCTTGGAAT GATCTGGACC ATCATCCTTC GTTTTGCCAT TCAGGATATC
    TCAGTTGAAG AGACATCTGC TAAAGAAGGA CTTTTGTTAT GGTGTCAGAG GAAGACAGCT
    CCCTACAAAA ATGTAAACAT CCAGAACTTC CATATCAGTT GGAAAGATGG CCTTGGTTTC
    TGCGCTTTAA TTCACAGACA TCGTCCAGAG CTCATTGATT ACGGGAAGCT ACGAAAGGAT
    GATCCTCTCA CTAATCTAAA CACAGCCTTT GATGTAGCTG AAAAATATCT GGATATCCCC
    AAGATGCTGG ATGCAGAAGA TATTATAGGC ACGCTAAGGC CGGATGAGAA GGCCATAATG
    ACCTATGTTT CATGTTACTA CCACGCCTTC TCAGGGGCAC AGAAGGCGGA GACAGCAGCA
    AATCGTATTT GCAAGGTTCT GGCAGTCAAC CAAGAGAATG AACAGCTCAT GGAAGACTAT
    GAAAAACTGG CCAGTGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC
    CGGGCACCAG AGAACACCAT GCAAGCCATG CAGCAGAAAC TGGAGGACTT CCGGGACTAC
    CGCCGCCTGC ATAAGCCCCC CAAAGTCCAA GAGAAGTGCC AACTTGAGAT CAATTTCAAC
    ACCTTGCAGA CCAAGCTACG GCTCAGCAAC AGGCCGGCCT TCATGCCTTC AGAAGGGAAA
    ATGGTTTCAG ATATAAACAA TGCCTGGGGT GGCCTAGAGC AGGCTGAGAA GGGCTATGAG
    GAGTGGTTGT TGAATGAGAT CCGGAGGCTA GAGAGGCTGG ATCACCTGGC AGAGAAGTTC
    CGGCAGAAAG CATCTATTCA CGAGTCCTGG ACAGATGGTA AGGAAGCGAT GCTGCAGCAG
    AAGGATTATG AAACTGCTAC CCTCTCGGAG ATAAAGGCCC TGCTGAAGAA ACACGAGGCC
    TTTGAGAGCG ACCTGGCTGC ACACCAGGAC CGCGTGGAAC AGATTGCTGC TATTGCACAA
    GAGCTGAATG AGCTGGACTA TTATGACTCT CCGAGTGTCA ATGCCAGGTG CCAGAAGATC
    TGTGATCAGT GGGATAATCT GGGTGCCCTA ACCCAGAAAC GTAGAGAAGC TTTAGAGAGG
    ACAGAGAAGC TACTGGAAAC AATTGACCAG TTGTACCTGG AATATGCCAA ACGAGCTGCC
    CCCTTCAATA ACTGGATGGA AGGGGCCATG GAGGACCTGC AGGACACGTT CATTGTTCAC
    ACCATTGAGG AGATCCAGGG ATTGACCACA GCCCACGAAC AGTTCAAAGC CACTTTGCCA
    GATGCCGACA AGGAGCGACA GGCCATCCTG GGAATCCACA ATGAAGTCTC AAAGATTGTC
    CAGACGTACC ACGTCAACAT GGCAGGAACG AACCCTTACA CTACAATCAC CCCACAGGAG
    ATCAATGGCA AATGGGAACA CGTGCGGCAG CTGGTTCCTA GAAGGGATCA AGCCCTAATG
    GAAGAACATG CTCGCCAGCA GCAAAATGAG CGACTTCGTA AGCAGTTTGG TGCACAAGCC
    AATGTCATTG GTCCCTGGAT TCAGACCAAG ATGGAGGAGA TTGGCCGCAT TTCAATAGAG
    ATGCACGGGA CTCTGGAGGA CCAGCTCAAC CACTTGCGGC AGTATGAGAA AAGCATTGTG
    AATTACAAAC CAAAGATCGA CCAGTTGGAA GGAGACCACC AACAGATCCA GGAGGCGCTT
    ATCTTTGACA ACAAGCACAC CAACTACACC ATGGAGCATA TTCGTGTGGG CTGGGAGCAG
    CTATTAACAA CAATTGCTAG AACCATTAAT GAAGTGGAAA ACCAGATTCT GACCCGTGAT
    GCCAAAGGAA TAAGCCAGGA ACAGATGAAT GAGTTCCGAG CCTCTTTCAA CCATTTTGAC
    AGGAAGAAGA CTGGTATGAT GGATTGTGAA GATTTCCGAG CCTGCCTTAT CTCCATGGGT
    TACAATATGG GAGAGGCAGA GTTTGCCCGC ATCATGAGCA TTGTCGATCC TAATCGCATG
    GGTGTAGTGA CGTTCCAGGC CTTCATTGAC TTCATGTCCC GGGAAACAGC AGATACAGAT
    ACTGCTGACC AGGTTATGGC TTCTTTCAAG ATCCTGGCTG GAGATAAGAA CTACATCACT
    GTGGATGAGT TACGGCGGGA GCTGCCTCCT GATCAGGCTG AGTACTGCAT TGCTAGAATG
    GCACCCTACA ATGGCCGTGA TGCTGTACCC GGTGCCCTGG ACTACATGTC GTTCTCCACG
    GCGCTCTATG GCGAAAGTGA CCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015142745.1
    CDS184..2850
    Translation

    Target ORF information:

    RefSeq Version XM_015287259.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus actinin, alpha 1 (ACTN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287259.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGATCATC ACTACGACCC GCAGCAGACC AACGATTACA TGCAGCCGGA GGAGGACTGG 
    GACCGGGACC TGCTCCTCGA CCCGGCTTGG GAGAAGCAGC AGCGGAAGAC ATTTACAGCA
    TGGTGTAATT CTCACCTCCG CAAGGCTGGG ACACAGATTG AGAACATAGA GGAAGATTTC
    AGGGATGGTC TTAAACTCAT GTTACTTCTG GAAGTCATTT CAGGTGAACG TTTGGCTAAG
    CCCGAAAGAG GCAAAATGCG AGTGCACAAA ATATCCAACG TGAACAAGGC CTTGGATTTC
    ATTGCCAGCA AAGGAGTCAA ACTAGTATCA ATTGGAGCAG AAGAAATTGT GGATGGCAAC
    GTTAAAATGA CTCTTGGAAT GATCTGGACC ATCATCCTTC GTTTTGCCAT TCAGGATATC
    TCAGTTGAAG AGACATCTGC TAAAGAAGGA CTTTTGTTAT GGTGTCAGAG GAAGACAGCT
    CCCTACAAAA ATGTAAACAT CCAGAACTTC CATATCAGTT GGAAAGATGG CCTTGGTTTC
    TGCGCTTTAA TTCACAGACA TCGTCCAGAG CTCATTGATT ACGGGAAGCT ACGAAAGGAT
    GATCCTCTCA CTAATCTAAA CACAGCCTTT GATGTAGCTG AAAAATATCT GGATATCCCC
    AAGATGCTGG ATGCAGAAGA TATTATAGGC ACGCTAAGGC CGGATGAGAA GGCCATAATG
    ACCTATGTTT CATGTTACTA CCACGCCTTC TCAGGGGCAC AGAAGGCGGA GACAGCAGCA
    AATCGTATTT GCAAGGTTCT GGCAGTCAAC CAAGAGAATG AACAGCTCAT GGAAGACTAT
    GAAAAACTGG CCAGTGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC
    CGGGCACCAG AGAACACCAT GCAAGCCATG CAGCAGAAAC TGGAGGACTT CCGGGACTAC
    CGCCGCCTGC ATAAGCCCCC CAAAGTCCAA GAGAAGTGCC AACTTGAGAT CAATTTCAAC
    ACCTTGCAGA CCAAGCTACG GCTCAGCAAC AGGCCGGCCT TCATGCCTTC AGAAGGGAAA
    ATGGTTTCAG ATATAAACAA TGCCTGGGGT GGCCTAGAGC AGGCTGAGAA GGGCTATGAG
    GAGTGGTTGT TGAATGAGAT CCGGAGGCTA GAGAGGCTGG ATCACCTGGC AGAGAAGTTC
    CGGCAGAAAG CATCTATTCA CGAGTCCTGG ACAGATGGTA AGGAAGCGAT GCTGCAGCAG
    AAGGATTATG AAACTGCTAC CCTCTCGGAG ATAAAGGCCC TGCTGAAGAA ACACGAGGCC
    TTTGAGAGCG ACCTGGCTGC ACACCAGGAC CGCGTGGAAC AGATTGCTGC TATTGCACAA
    GAGCTGAATG AGCTGGACTA TTATGACTCT CCGAGTGTCA ATGCCAGGTG CCAGAAGATC
    TGTGATCAGT GGGATAATCT GGGTGCCCTA ACCCAGAAAC GTAGAGAAGC TTTAGAGAGG
    ACAGAGAAGC TACTGGAAAC AATTGACCAG TTGTACCTGG AATATGCCAA ACGAGCTGCC
    CCCTTCAATA ACTGGATGGA AGGGGCCATG GAGGACCTGC AGGACACGTT CATTGTTCAC
    ACCATTGAGG AGATCCAGGG ATTGACCACA GCCCACGAAC AGTTCAAAGC CACTTTGCCA
    GATGCCGACA AGGAGCGACA GGCCATCCTG GGAATCCACA ATGAAGTCTC AAAGATTGTC
    CAGACGTACC ACGTCAACAT GGCAGGAACG AACCCTTACA CTACAATCAC CCCACAGGAG
    ATCAATGGCA AATGGGAACA CGTGCGGCAG CTGGTTCCTA GAAGGGATCA AGCCCTAATG
    GAAGAACATG CTCGCCAGCA GCAAAATGAG CGACTTCGTA AGCAGTTTGG TGCACAAGCC
    AATGTCATTG GTCCCTGGAT TCAGACCAAG ATGGAGGAGA TTGGCCGCAT TTCAATAGAG
    ATGCACGGGA CTCTGGAGGA CCAGCTCAAC CACTTGCGGC AGTATGAGAA AAGCATTGTG
    AATTACAAAC CAAAGATCGA CCAGTTGGAA GGAGACCACC AACAGATCCA GGAGGCGCTT
    ATCTTTGACA ACAAGCACAC CAACTACACC ATGGAGCATA TTCGTGTGGG CTGGGAGCAG
    CTATTAACAA CAATTGCTAG AACCATTAAT GAAGTGGAAA ACCAGATTCT GACCCGTGAT
    GCCAAAGGAA TAAGCCAGGA ACAGATGAAT GAGTTCCGAG CCTCTTTCAA CCATTTTGAC
    AGGAAGAAGA CTGGTATGAT GGATTGTGAA GATTTCCGAG CCTGCCTTAT CTCCATGGGT
    TACAATATGG GAGAGGCAGA GTTTGCCCGC ATCATGAGCA TTGTCGATCC TAATCGCATG
    GGTGTAGTGA CGTTCCAGGC CTTCATTGAC TTCATGTCCC GGGAAACAGC AGATACAGAT
    ACTGCTGACC AGGTTATGGC TTCTTTCAAG ATCCTGGCTG GAGATAAGAA CTACATCACT
    GTGGATGAGT TACGGCGGGA GCTGCCTCCT GATCAGGCTG AGTACTGCAT TGCTAGAATG
    GCACCCTACA ATGGCCGTGA TGCTGTACCC GGTGCCCTGG ACTACATGTC GTTCTCCACG
    GCGCTCTATG GCGAAAGTGA CCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa36739
    Clone ID Related Accession (Same CDS sequence) XM_015287260.1
    Accession Version XM_015287260.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2289bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product alpha-actinin-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGTGTCAG AGGAAGACAG CTCCCTACAA AATGTAAACA TCCAGAACTT CCATATCAGT 
    TGGAAAGATG GCCTTGGTTT CTGCGCTTTA ATTCACAGAC ATCGTCCAGA GCTCATTGAT
    TACGGGAAGC TACGAAAGGA TGATCCTCTC ACTAATCTAA ACACAGCCTT TGATGTAGCT
    GAAAAATATC TGGATATCCC CAAGATGCTG GATGCAGAAG ACATTGTTGG AACTGCCCGT
    CCTGACGAGA AAGCCATCAT GACCTATGTT TCTAGCTTCT ACCACGCCTT CTCAGGAGCC
    CAGAAGGCGG AGACAGCAGC AAATCGTATT TGCAAGGTTC TGGCAGTCAA CCAAGAGAAT
    GAACAGCTCA TGGAAGACTA TGAAAAACTG GCCAGTGATC TGCTGGAGTG GATCCGTCGC
    ACCATCCCCT GGCTGGAGAA CCGGGCACCA GAGAACACCA TGCAAGCCAT GCAGCAGAAA
    CTGGAGGACT TCCGGGACTA CCGCCGCCTG CATAAGCCCC CCAAAGTCCA AGAGAAGTGC
    CAACTTGAGA TCAATTTCAA CACCTTGCAG ACCAAGCTAC GGCTCAGCAA CAGGCCGGCC
    TTCATGCCTT CAGAAGGGAA AATGGTTTCA GATATAAACA ATGCCTGGGG TGGCCTAGAG
    CAGGCTGAGA AGGGCTATGA GGAGTGGTTG TTGAATGAGA TCCGGAGGCT AGAGAGGCTG
    GATCACCTGG CAGAGAAGTT CCGGCAGAAA GCATCTATTC ACGAGTCCTG GACAGATGGT
    AAGGAAGCGA TGCTGCAGCA GAAGGATTAT GAAACTGCTA CCCTCTCGGA GATAAAGGCC
    CTGCTGAAGA AACACGAGGC CTTTGAGAGC GACCTGGCTG CACACCAGGA CCGCGTGGAA
    CAGATTGCTG CTATTGCACA AGAGCTGAAT GAGCTGGACT ATTATGACTC TCCGAGTGTC
    AATGCCAGGT GCCAGAAGAT CTGTGATCAG TGGGATAATC TGGGTGCCCT AACCCAGAAA
    CGTAGAGAAG CTTTAGAGAG GACAGAGAAG CTACTGGAAA CAATTGACCA GTTGTACCTG
    GAATATGCCA AACGAGCTGC CCCCTTCAAT AACTGGATGG AAGGGGCCAT GGAGGACCTG
    CAGGACACGT TCATTGTTCA CACCATTGAG GAGATCCAGG GATTGACCAC AGCCCACGAA
    CAGTTCAAAG CCACTTTGCC AGATGCCGAC AAGGAGCGAC AGGCCATCCT GGGAATCCAC
    AATGAAGTCT CAAAGATTGT CCAGACGTAC CACGTCAACA TGGCAGGAAC GAACCCTTAC
    ACTACAATCA CCCCACAGGA GATCAATGGC AAATGGGAAC ACGTGCGGCA GCTGGTTCCT
    AGAAGGGATC AAGCCCTAAT GGAAGAACAT GCTCGCCAGC AGCAAAATGA GCGACTTCGT
    AAGCAGTTTG GTGCACAAGC CAATGTCATT GGTCCCTGGA TTCAGACCAA GATGGAGGAG
    ATTGGCCGCA TTTCAATAGA GATGCACGGG ACTCTGGAGG ACCAGCTCAA CCACTTGCGG
    CAGTATGAGA AAAGCATTGT GAATTACAAA CCAAAGATCG ACCAGTTGGA AGGAGACCAC
    CAACAGATCC AGGAGGCGCT TATCTTTGAC AACAAGCACA CCAACTACAC CATGGAGCAT
    ATTCGTGTGG GCTGGGAGCA GCTATTAACA ACAATTGCTA GAACCATTAA TGAAGTGGAA
    AACCAGATTC TGACCCGTGA TGCCAAAGGA ATAAGCCAGG AACAGATGAA TGAGTTCCGA
    GCCTCTTTCA ACCATTTTGA CAGGGACCAC TCCGGCACAC TTGGCCCTGA GGAATTCAAA
    GCCTGTCTCA TCAGCTTGGG TTACGATATT GGAAACGATG CACAGAAGAA GACTGGTATG
    ATGGATTGTG AAGATTTCCG AGCCTGCCTT ATCTCCATGG GTTACAATAT GGGAGAGGCA
    GAGTTTGCCC GCATCATGAG CATTGTCGAT CCTAATCGCA TGGGTGTAGT GACGTTCCAG
    GCCTTCATTG ACTTCATGTC CCGGGAAACA GCAGATACAG ATACTGCTGA CCAGGTTATG
    GCTTCTTTCA AGATCCTGGC TGGAGATAAG AACTACATCA CTGTGGATGA GTTACGGCGG
    GAGCTGCCTC CTGATCAGGC TGAGTACTGC ATTGCTAGAA TGGCACCCTA CAATGGCCGT
    GATGCTGTAC CCGGTGCCCT GGACTACATG TCGTTCTCCA CGGCGCTCTA TGGCGAAAGT
    GACCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015142746.1
    CDS26..2314
    Translation

    Target ORF information:

    RefSeq Version XM_015287260.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus actinin, alpha 1 (ACTN1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287260.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGTGTCAG AGGAAGACAG CTCCCTACAA AATGTAAACA TCCAGAACTT CCATATCAGT 
    TGGAAAGATG GCCTTGGTTT CTGCGCTTTA ATTCACAGAC ATCGTCCAGA GCTCATTGAT
    TACGGGAAGC TACGAAAGGA TGATCCTCTC ACTAATCTAA ACACAGCCTT TGATGTAGCT
    GAAAAATATC TGGATATCCC CAAGATGCTG GATGCAGAAG ACATTGTTGG AACTGCCCGT
    CCTGACGAGA AAGCCATCAT GACCTATGTT TCTAGCTTCT ACCACGCCTT CTCAGGAGCC
    CAGAAGGCGG AGACAGCAGC AAATCGTATT TGCAAGGTTC TGGCAGTCAA CCAAGAGAAT
    GAACAGCTCA TGGAAGACTA TGAAAAACTG GCCAGTGATC TGCTGGAGTG GATCCGTCGC
    ACCATCCCCT GGCTGGAGAA CCGGGCACCA GAGAACACCA TGCAAGCCAT GCAGCAGAAA
    CTGGAGGACT TCCGGGACTA CCGCCGCCTG CATAAGCCCC CCAAAGTCCA AGAGAAGTGC
    CAACTTGAGA TCAATTTCAA CACCTTGCAG ACCAAGCTAC GGCTCAGCAA CAGGCCGGCC
    TTCATGCCTT CAGAAGGGAA AATGGTTTCA GATATAAACA ATGCCTGGGG TGGCCTAGAG
    CAGGCTGAGA AGGGCTATGA GGAGTGGTTG TTGAATGAGA TCCGGAGGCT AGAGAGGCTG
    GATCACCTGG CAGAGAAGTT CCGGCAGAAA GCATCTATTC ACGAGTCCTG GACAGATGGT
    AAGGAAGCGA TGCTGCAGCA GAAGGATTAT GAAACTGCTA CCCTCTCGGA GATAAAGGCC
    CTGCTGAAGA AACACGAGGC CTTTGAGAGC GACCTGGCTG CACACCAGGA CCGCGTGGAA
    CAGATTGCTG CTATTGCACA AGAGCTGAAT GAGCTGGACT ATTATGACTC TCCGAGTGTC
    AATGCCAGGT GCCAGAAGAT CTGTGATCAG TGGGATAATC TGGGTGCCCT AACCCAGAAA
    CGTAGAGAAG CTTTAGAGAG GACAGAGAAG CTACTGGAAA CAATTGACCA GTTGTACCTG
    GAATATGCCA AACGAGCTGC CCCCTTCAAT AACTGGATGG AAGGGGCCAT GGAGGACCTG
    CAGGACACGT TCATTGTTCA CACCATTGAG GAGATCCAGG GATTGACCAC AGCCCACGAA
    CAGTTCAAAG CCACTTTGCC AGATGCCGAC AAGGAGCGAC AGGCCATCCT GGGAATCCAC
    AATGAAGTCT CAAAGATTGT CCAGACGTAC CACGTCAACA TGGCAGGAAC GAACCCTTAC
    ACTACAATCA CCCCACAGGA GATCAATGGC AAATGGGAAC ACGTGCGGCA GCTGGTTCCT
    AGAAGGGATC AAGCCCTAAT GGAAGAACAT GCTCGCCAGC AGCAAAATGA GCGACTTCGT
    AAGCAGTTTG GTGCACAAGC CAATGTCATT GGTCCCTGGA TTCAGACCAA GATGGAGGAG
    ATTGGCCGCA TTTCAATAGA GATGCACGGG ACTCTGGAGG ACCAGCTCAA CCACTTGCGG
    CAGTATGAGA AAAGCATTGT GAATTACAAA CCAAAGATCG ACCAGTTGGA AGGAGACCAC
    CAACAGATCC AGGAGGCGCT TATCTTTGAC AACAAGCACA CCAACTACAC CATGGAGCAT
    ATTCGTGTGG GCTGGGAGCA GCTATTAACA ACAATTGCTA GAACCATTAA TGAAGTGGAA
    AACCAGATTC TGACCCGTGA TGCCAAAGGA ATAAGCCAGG AACAGATGAA TGAGTTCCGA
    GCCTCTTTCA ACCATTTTGA CAGGGACCAC TCCGGCACAC TTGGCCCTGA GGAATTCAAA
    GCCTGTCTCA TCAGCTTGGG TTACGATATT GGAAACGATG CACAGAAGAA GACTGGTATG
    ATGGATTGTG AAGATTTCCG AGCCTGCCTT ATCTCCATGG GTTACAATAT GGGAGAGGCA
    GAGTTTGCCC GCATCATGAG CATTGTCGAT CCTAATCGCA TGGGTGTAGT GACGTTCCAG
    GCCTTCATTG ACTTCATGTC CCGGGAAACA GCAGATACAG ATACTGCTGA CCAGGTTATG
    GCTTCTTTCA AGATCCTGGC TGGAGATAAG AACTACATCA CTGTGGATGA GTTACGGCGG
    GAGCTGCCTC CTGATCAGGC TGAGTACTGC ATTGCTAGAA TGGCACCCTA CAATGGCCGT
    GATGCTGTAC CCGGTGCCCT GGACTACATG TCGTTCTCCA CGGCGCTCTA TGGCGAAAGT
    GACCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa36740
    Clone ID Related Accession (Same CDS sequence) XM_015287261.1
    Accession Version XM_015287261.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2289bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product alpha-actinin-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGTGTCAG AGGAAGACAG CTCCCTACAA AATGTAAACA TCCAGAACTT CCATATCAGT 
    TGGAAAGATG GCCTTGGTTT CTGCGCTTTA ATTCACAGAC ATCGTCCAGA GCTCATTGAT
    TACGGGAAGC TACGAAAGGA TGATCCTCTC ACTAATCTAA ACACAGCCTT TGATGTAGCT
    GAAAAATATC TGGATATCCC CAAGATGCTG GATGCAGAAG ATATTATAGG CACGCTAAGG
    CCGGATGAGA AGGCCATAAT GACCTATGTT TCATGTTACT ACCACGCCTT CTCAGGGGCA
    CAGAAGGCGG AGACAGCAGC AAATCGTATT TGCAAGGTTC TGGCAGTCAA CCAAGAGAAT
    GAACAGCTCA TGGAAGACTA TGAAAAACTG GCCAGTGATC TGCTGGAGTG GATCCGTCGC
    ACCATCCCCT GGCTGGAGAA CCGGGCACCA GAGAACACCA TGCAAGCCAT GCAGCAGAAA
    CTGGAGGACT TCCGGGACTA CCGCCGCCTG CATAAGCCCC CCAAAGTCCA AGAGAAGTGC
    CAACTTGAGA TCAATTTCAA CACCTTGCAG ACCAAGCTAC GGCTCAGCAA CAGGCCGGCC
    TTCATGCCTT CAGAAGGGAA AATGGTTTCA GATATAAACA ATGCCTGGGG TGGCCTAGAG
    CAGGCTGAGA AGGGCTATGA GGAGTGGTTG TTGAATGAGA TCCGGAGGCT AGAGAGGCTG
    GATCACCTGG CAGAGAAGTT CCGGCAGAAA GCATCTATTC ACGAGTCCTG GACAGATGGT
    AAGGAAGCGA TGCTGCAGCA GAAGGATTAT GAAACTGCTA CCCTCTCGGA GATAAAGGCC
    CTGCTGAAGA AACACGAGGC CTTTGAGAGC GACCTGGCTG CACACCAGGA CCGCGTGGAA
    CAGATTGCTG CTATTGCACA AGAGCTGAAT GAGCTGGACT ATTATGACTC TCCGAGTGTC
    AATGCCAGGT GCCAGAAGAT CTGTGATCAG TGGGATAATC TGGGTGCCCT AACCCAGAAA
    CGTAGAGAAG CTTTAGAGAG GACAGAGAAG CTACTGGAAA CAATTGACCA GTTGTACCTG
    GAATATGCCA AACGAGCTGC CCCCTTCAAT AACTGGATGG AAGGGGCCAT GGAGGACCTG
    CAGGACACGT TCATTGTTCA CACCATTGAG GAGATCCAGG GATTGACCAC AGCCCACGAA
    CAGTTCAAAG CCACTTTGCC AGATGCCGAC AAGGAGCGAC AGGCCATCCT GGGAATCCAC
    AATGAAGTCT CAAAGATTGT CCAGACGTAC CACGTCAACA TGGCAGGAAC GAACCCTTAC
    ACTACAATCA CCCCACAGGA GATCAATGGC AAATGGGAAC ACGTGCGGCA GCTGGTTCCT
    AGAAGGGATC AAGCCCTAAT GGAAGAACAT GCTCGCCAGC AGCAAAATGA GCGACTTCGT
    AAGCAGTTTG GTGCACAAGC CAATGTCATT GGTCCCTGGA TTCAGACCAA GATGGAGGAG
    ATTGGCCGCA TTTCAATAGA GATGCACGGG ACTCTGGAGG ACCAGCTCAA CCACTTGCGG
    CAGTATGAGA AAAGCATTGT GAATTACAAA CCAAAGATCG ACCAGTTGGA AGGAGACCAC
    CAACAGATCC AGGAGGCGCT TATCTTTGAC AACAAGCACA CCAACTACAC CATGGAGCAT
    ATTCGTGTGG GCTGGGAGCA GCTATTAACA ACAATTGCTA GAACCATTAA TGAAGTGGAA
    AACCAGATTC TGACCCGTGA TGCCAAAGGA ATAAGCCAGG AACAGATGAA TGAGTTCCGA
    GCCTCTTTCA ACCATTTTGA CAGGGACCAC TCCGGCACAC TTGGCCCTGA GGAATTCAAA
    GCCTGTCTCA TCAGCTTGGG TTACGATATT GGAAACGATG CACAGAAGAA GACTGGTATG
    ATGGATTGTG AAGATTTCCG AGCCTGCCTT ATCTCCATGG GTTACAATAT GGGAGAGGCA
    GAGTTTGCCC GCATCATGAG CATTGTCGAT CCTAATCGCA TGGGTGTAGT GACGTTCCAG
    GCCTTCATTG ACTTCATGTC CCGGGAAACA GCAGATACAG ATACTGCTGA CCAGGTTATG
    GCTTCTTTCA AGATCCTGGC TGGAGATAAG AACTACATCA CTGTGGATGA GTTACGGCGG
    GAGCTGCCTC CTGATCAGGC TGAGTACTGC ATTGCTAGAA TGGCACCCTA CAATGGCCGT
    GATGCTGTAC CCGGTGCCCT GGACTACATG TCGTTCTCCA CGGCGCTCTA TGGCGAAAGT
    GACCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015142747.1
    CDS27..2315
    Translation

    Target ORF information:

    RefSeq Version XM_015287261.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus actinin, alpha 1 (ACTN1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287261.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGTGTCAG AGGAAGACAG CTCCCTACAA AATGTAAACA TCCAGAACTT CCATATCAGT 
    TGGAAAGATG GCCTTGGTTT CTGCGCTTTA ATTCACAGAC ATCGTCCAGA GCTCATTGAT
    TACGGGAAGC TACGAAAGGA TGATCCTCTC ACTAATCTAA ACACAGCCTT TGATGTAGCT
    GAAAAATATC TGGATATCCC CAAGATGCTG GATGCAGAAG ATATTATAGG CACGCTAAGG
    CCGGATGAGA AGGCCATAAT GACCTATGTT TCATGTTACT ACCACGCCTT CTCAGGGGCA
    CAGAAGGCGG AGACAGCAGC AAATCGTATT TGCAAGGTTC TGGCAGTCAA CCAAGAGAAT
    GAACAGCTCA TGGAAGACTA TGAAAAACTG GCCAGTGATC TGCTGGAGTG GATCCGTCGC
    ACCATCCCCT GGCTGGAGAA CCGGGCACCA GAGAACACCA TGCAAGCCAT GCAGCAGAAA
    CTGGAGGACT TCCGGGACTA CCGCCGCCTG CATAAGCCCC CCAAAGTCCA AGAGAAGTGC
    CAACTTGAGA TCAATTTCAA CACCTTGCAG ACCAAGCTAC GGCTCAGCAA CAGGCCGGCC
    TTCATGCCTT CAGAAGGGAA AATGGTTTCA GATATAAACA ATGCCTGGGG TGGCCTAGAG
    CAGGCTGAGA AGGGCTATGA GGAGTGGTTG TTGAATGAGA TCCGGAGGCT AGAGAGGCTG
    GATCACCTGG CAGAGAAGTT CCGGCAGAAA GCATCTATTC ACGAGTCCTG GACAGATGGT
    AAGGAAGCGA TGCTGCAGCA GAAGGATTAT GAAACTGCTA CCCTCTCGGA GATAAAGGCC
    CTGCTGAAGA AACACGAGGC CTTTGAGAGC GACCTGGCTG CACACCAGGA CCGCGTGGAA
    CAGATTGCTG CTATTGCACA AGAGCTGAAT GAGCTGGACT ATTATGACTC TCCGAGTGTC
    AATGCCAGGT GCCAGAAGAT CTGTGATCAG TGGGATAATC TGGGTGCCCT AACCCAGAAA
    CGTAGAGAAG CTTTAGAGAG GACAGAGAAG CTACTGGAAA CAATTGACCA GTTGTACCTG
    GAATATGCCA AACGAGCTGC CCCCTTCAAT AACTGGATGG AAGGGGCCAT GGAGGACCTG
    CAGGACACGT TCATTGTTCA CACCATTGAG GAGATCCAGG GATTGACCAC AGCCCACGAA
    CAGTTCAAAG CCACTTTGCC AGATGCCGAC AAGGAGCGAC AGGCCATCCT GGGAATCCAC
    AATGAAGTCT CAAAGATTGT CCAGACGTAC CACGTCAACA TGGCAGGAAC GAACCCTTAC
    ACTACAATCA CCCCACAGGA GATCAATGGC AAATGGGAAC ACGTGCGGCA GCTGGTTCCT
    AGAAGGGATC AAGCCCTAAT GGAAGAACAT GCTCGCCAGC AGCAAAATGA GCGACTTCGT
    AAGCAGTTTG GTGCACAAGC CAATGTCATT GGTCCCTGGA TTCAGACCAA GATGGAGGAG
    ATTGGCCGCA TTTCAATAGA GATGCACGGG ACTCTGGAGG ACCAGCTCAA CCACTTGCGG
    CAGTATGAGA AAAGCATTGT GAATTACAAA CCAAAGATCG ACCAGTTGGA AGGAGACCAC
    CAACAGATCC AGGAGGCGCT TATCTTTGAC AACAAGCACA CCAACTACAC CATGGAGCAT
    ATTCGTGTGG GCTGGGAGCA GCTATTAACA ACAATTGCTA GAACCATTAA TGAAGTGGAA
    AACCAGATTC TGACCCGTGA TGCCAAAGGA ATAAGCCAGG AACAGATGAA TGAGTTCCGA
    GCCTCTTTCA ACCATTTTGA CAGGGACCAC TCCGGCACAC TTGGCCCTGA GGAATTCAAA
    GCCTGTCTCA TCAGCTTGGG TTACGATATT GGAAACGATG CACAGAAGAA GACTGGTATG
    ATGGATTGTG AAGATTTCCG AGCCTGCCTT ATCTCCATGG GTTACAATAT GGGAGAGGCA
    GAGTTTGCCC GCATCATGAG CATTGTCGAT CCTAATCGCA TGGGTGTAGT GACGTTCCAG
    GCCTTCATTG ACTTCATGTC CCGGGAAACA GCAGATACAG ATACTGCTGA CCAGGTTATG
    GCTTCTTTCA AGATCCTGGC TGGAGATAAG AACTACATCA CTGTGGATGA GTTACGGCGG
    GAGCTGCCTC CTGATCAGGC TGAGTACTGC ATTGCTAGAA TGGCACCCTA CAATGGCCGT
    GATGCTGTAC CCGGTGCCCT GGACTACATG TCGTTCTCCA CGGCGCTCTA TGGCGAAAGT
    GACCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.