IRS2 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	IRS2
Entrez Gene ID	101749060
Full Name	insulin receptor substrate 2
General protein information	Preferred Names insulin receptor substrate 2 Names insulin receptor substrate 2
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015277882.2	XP_015133368.1	insulin receptor substrate 2 isoform X1
XM_015277883.2	XP_015133369.1	insulin receptor substrate 2 isoform X2
XM_015277883.1	XP_015133369.1	insulin receptor substrate 2 isoform X2
XM_015277882.1	XP_015133368.1	insulin receptor substrate 2 isoform X1
XM_015277884.1	XP_015133370.1	insulin receptor substrate 2 isoform X3
XM_015277886.1	XP_015133372.1	insulin receptor substrate 2 isoform X4
XM_015277886.2	XP_015133372.1	insulin receptor substrate 2 isoform X4
XM_015277884.2	XP_015133370.1	insulin receptor substrate 2 isoform X3

Pathways from BioSystems
Gene Ontology

The following IRS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IRS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa30699	XM_015277882.2 Latest version!	Gallus gallus insulin receptor substrate 2 (IRS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OGa30700	XM_015277883.2 Latest version!	Gallus gallus insulin receptor substrate 2 (IRS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OGa30701	XM_015277884.2 Latest version!	Gallus gallus insulin receptor substrate 2 (IRS2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OGa30702	XM_015277886.2 Latest version!	Gallus gallus insulin receptor substrate 2 (IRS2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
View Old Accession Versions >>
OGa30700	XM_015277883.1	Gallus gallus uncharacterized LOC101749060 (LOC101749060), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OGa30699	XM_015277882.1	Gallus gallus uncharacterized LOC101749060 (LOC101749060), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OGa30702	XM_015277886.1	Gallus gallus uncharacterized LOC101749060 (LOC101749060), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OGa30701	XM_015277884.1	Gallus gallus uncharacterized LOC101749060 (LOC101749060), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IRS2 ( XM_015277882.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133368.1 Gallus gallus insulin receptor substrate 2 (IRS2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa30699
Clone ID Related Accession (Same CDS sequence)	XM_015277882.2 , XM_015277882.1
Accession Version	XM_015277882.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	insulin receptor substrate 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277882.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCC CCGCCGCGCC GGGGCTGCCG CCCGGCCCCA ACCTGAACAA CAACAACAAC 
AACCGCGGCG TGAGGAAGTG CGGCTACCTG CGCAAGCAGA AGCACGGCCA CAAGCGCTTC 
TTCGTGCTGC GCGGCCCGGG CGGCGGCGGG GAGGAGGCGG GGGGCGCCCG GCTGGAGTAC 
TACGAGAGCG AGAAGAAATG GAGGAACAAG TCCGGGGCGC CCAAGCGGGT GATCGCGCTG 
GACTCGTGCC TCAACATCAA CAAGCGGGCG GACGCCAAGC ACAAGTACCT CATCGCCCTC 
TATACCAAGG ACGAGTACTT TGCCGTGGCG GCCGAGAACG AGCAGGAGCA GGAGGGCTGG 
TACCGCGCCC TCACCGACCT GCTGAGCGAG GGCAAGGCGG CCGCCGGCTC CCCGCACCGC 
CCCCTGCCCT CGCCCTTCGC CGCGCTGGCC GCCGGCGAGG AGCCCGACTA CGGGCTGATG 
ACGCCGGCCG CCGCCGCCTA CCGGGAGGTC TGGCAGGTGA CGCTGAAGCC CAAAGGCTTG 
GGGCAGAGCA AGAACCTCAC CGGCGTGCAC CGCCTCTGCC TCTCGGCCCG CACCATCGGC 
TTCGTGCGCC TCAACTGCGA GCTGCCCTCG GTCACGCTGC AGCTGATGAA CATCCGCCGC 
TGCGGCCACT CGGACAGCTT CTTCTTCATC GAGGTGGGCC GTTCGGCCGC CACCGGGCCC 
GGCGAGCTGT GGATGCAGGC GGACGACTCG GTGGTGGCCC AGAACATCCA CGAGACCATC 
CTGGAGGCTA TGAAGGCTCT AAAGGAGCTG TCCGAGTTCC GGCCCCGCAG CAAGAGCCAG 
TCGTCGTCCT CCTCTTCCTC CGGGGGGGCG GCCGGAGGCG GCGGCCCCTC GGCCACGCAC 
CCCATCACCG TGCCGGGCCG CCGGCACCAC CACCTGGTCA ACCTGCCGCC CAGCCAGACC 
GGCCTGCTCC GCCGATCCCG CACCGACAGC CTGGCCGCCG GCAGCAGGGC CGCGCCGTGC 
CGGGTGCGGA CGGCCAGCGA GGGCGACGGC TGCCGGGTGG GCTCGGCCGC CGGCAGCCCC 
ATGAGCCCCG GCCCCGTGCG GACCCCGCTC AGCCGCTCGC ACACGCTGAG CGGCGGCGGA 
GGGCGGCCGG CGGGCAAGCT GCTGCCCGTG CTGGCCGGGG GCGGGCTGCA AAGCAGCCGC 
TCCATGTCCA TGCCCGCCTC CCACTCGCCC CCCTCCGCCA CTAGCCCCGT CAGTCTCTCC 
TCCAGCAGCG GCCTGGGCTC CGAGCCGGCC CACCCGCATC ACCCGCAGCG CCCGTCCAGC 
GGCAGCGCCT CGGTGTCCGG CTCGCCCAGC GACGCGGGCT TCATGTCCTT CGACGAGTAC 
GGCTCCAGCC CGGGCGGCGA CCCGCGGCCC TTCTCCTCCT CCTCCACCGC CAGCAACCGC 
AGCAACACAC CCGAATCGGT GGCCGAGACC CCCCCGGTGC GGGACTCCGG CGGCGGCACC 
GATCTGTATG GCTACATGGC GATGGAGCGG CCCCCGAGCG GCCGTCTCTG CTACCGGCCT 
TGCCCCGAGG CTGACCGGGG CCACCGAAAG CGGACCTACT CGCTGACCAC CCCGTGCCGG 
CAGCGGCCCG CCCCGCCGCA GGTCTCCTCC GCCTCCCTGG ACGAGTACAC GCTAATGAGG 
GCCACCTTCG CCGGCAGCGC CGGCCGCCTC TTCCCCTCCT GCCCGGCAGG GGCTTCCCCT 
AAAGTCACCT ACACGCCGTA CCCCGAGGAC TACGGCGACA TCGAGATCGG CTCGCACCGC 
AGCTCCGGCA GCAGCAGCAC CAACCTGGGG CCGCCGGCAG GGGGGGGAGG CGGAGGCAGG 
GGCGGAGATG ACGATGGCTA CATGCCTATG ACCCCCGGCG TGGCCGCGGC CCTGGGTCGG 
GGGGGGCGGG GTGGCGATGA CTACATGCCC ATGAGCCCCA CCAGCGTGTC GGCCCCCAAA 
CAGATCCTGC AGCCCCGAGC GGGGGTGGGA GGCGGCTCCC CCGGCAACGG GAGCAGCTAT 
AAGACCAGCT CGCCGGGGGA AAGCTCCCCC GACGACAGCG GCTACATGAG GATGTGGTGC 
GGCTCTAAGC TGTCCGTGGA GAGTTCGGAC GGGAGGCTGA GCAACGGCGA CTACATCAAC 
ATGTCCCCTC GAGACCCCCA GCACGGGTCC CAGGCTCCCT CCCTCACCCC CCCGGACTTT 
TTCTTCGCCC CCGCAGGGCA CGGGGCTGGC GAGCCCCTCA AACCCGGCTG CTACTCTTAC 
AGCTCCCTGC CCCGCTCCTA CAAGAGCCAG GGCCCAGCTA AGGACAGCGA CCAGTACGTG 
TTCATGAACT CTCCGGGCAG GATGATCCCG GAGGAGGCGA TGTGCGGAGC GGTTCTGTCC 
CCAGCCGGCA CCTTCGCCCC CTCCAGCCAC ACGGTGCCTT CGCCCCTGCG GCACAGCCGG 
ACCGAGGGCT TCCTCAGCCA GCGGTGCCAG CGGGCCATCC GGCCCAGCCG CCTCTCTCTG 
GAGACCTTGC GGACGATGCT GCCAAGCATG AACGAGCACC CCTTGCCTCC CGAGCCCAAG 
AGCCCCGGGG AATACATCAA CATTGACTTC GGGGACACCG CTGTCTATTC TCCCCCCTCG 
CTGCCCACCG ACAGCCCAGC CTCCTCCCTG GGCTCAGGCA CAGGGCAGAG GCGCTCCCCT 
CTCTCCGACT ACATGAACAT CGACTTCAGC TCGCAGTCAC CCTCCCAGTC AGGCACAGTC 
TCGGTGGGCT CTTTGGAAGC GCTCTCGCCT GGCTCTTCCT CCAGCACCAG TCAGCCCGAT 
GGGCGCTACA TGAAGGCGGC TGGGGGGGTG GCCTGTCTGT CCAGCCCATC TGACAGCGGG 
GATTACACTG AGATGACCTT TGGCATGGCC ACTACCCCAC CCCAACCCAT CGTTCAGAAG 
CCAGAAAGTG CCCGGGTCAC CAGCCCCACG GCCGGGGTGA AGAGGCTCAC CCTCTCCGGG 
GTGGAGGCCT TCATTCTTTC CAGCCCACCC CCAGACCCCA ACCGCGGGGC CAAGGTCATT 
CGGGCTGACC CCCAGGGGCG CAGGAGGCAC AGCTCGGAAA CTTTCTCCTC CACCACCACT 
GTGACCCCCG TGTCCCCCTC CTTTGCACAC AACCCCAAAA GGCACAACTC AGCCTCGGTG 
GAGAACGTGT CCCTCAGGAA AAGCGAAGGC CTGGAGGAGG AGCAGGGCAG CAGCCCCATG 
TGCCGGGAGA CCTCGGCTGG CTTCCAGAAT GGCCTCAACT ACATCGCCGT CGACTTGGTG 
GACGGCAGCC TGGCAGGCTG TGACAAAGCC AGGTCAAAAG CCAGGCATGT CCTGAACGGA 
GGTGTCAACG GAGTGGAGAT GAGCGCCTAT GCCAGCATAG ACTTTCTGTC TCACAACCTG 
AAAGAAGCAA GCGCTGTGAA AGGGCTTCAT GCGGAAGACC AAGTCCAGGA GAAAACCTAT 
GCTTGGAAAA AGGCCTTGGC TGGCTTTAAC ATGGTCCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133368.1
CDS	189..3710
Translation	MASPAAPGLPPGPNLNNNNNNRGVRKCGYLRKQKHGHKRFFVLRGPGGGGEEAGGARLEYYESEKKWRNKSGAPKRVIALDSCLNINKRADAKHKYLIALYTKDEYFAVAAENEQEQEGWYRALTDLLSEGKAAAGSPHRPLPSPFAALAAGEEPDYGLMTPAAAAYREVWQVTLKPKGLGQSKNLTGVHRLCLSARTIGFVRLNCELPSVTLQLMNIRRCGHSDSFFFIEVGRSAATGPGELWMQADDSVVAQNIHETILEAMKALKELSEFRPRSKSQSSSSSSSGGAAGGGGPSATHPITVPGRRHHHLVNLPPSQTGLLRRSRTDSLAAGSRAAPCRVRTASEGDGCRVGSAAGSPMSPGPVRTPLSRSHTLSGGGGRPAGKLLPVLAGGGLQSSRSMSMPASHSPPSATSPVSLSSSSGLGSEPAHPHHPQRPSSGSASVSGSPSDAGFMSFDEYGSSPGGDPRPFSSSSTASNRSNTPESVAETPPVRDSGGGTDLYGYMAMERPPSGRLCYRPCPEADRGHRKRTYSLTTPCRQRPAPPQVSSASLDEYTLMRATFAGSAGRLFPSCPAGASPKVTYTPYPEDYGDIEIGSHRSSGSSSTNLGPPAGGGGGGRGGDDDGYMPMTPGVAAALGRGGRGGDDYMPMSPTSVSAPKQILQPRAGVGGGSPGNGSSYKTSSPGESSPDDSGYMRMWCGSKLSVESSDGRLSNGDYINMSPRDPQHGSQAPSLTPPDFFFAPAGHGAGEPLKPGCYSYSSLPRSYKSQGPAKDSDQYVFMNSPGRMIPEEAMCGAVLSPAGTFAPSSHTVPSPLRHSRTEGFLSQRCQRAIRPSRLSLETLRTMLPSMNEHPLPPEPKSPGEYINIDFGDTAVYSPPSLPTDSPASSLGSGTGQRRSPLSDYMNIDFSSQSPSQSGTVSVGSLEALSPGSSSSTSQPDGRYMKAAGGVACLSSPSDSGDYTEMTFGMATTPPQPIVQKPESARVTSPTAGVKRLTLSGVEAFILSSPPPDPNRGAKVIRADPQGRRRHSSETFSSTTTVTPVSPSFAHNPKRHNSASVENVSLRKSEGLEEEQGSSPMCRETSAGFQNGLNYIAVDLVDGSLAGCDKARSKARHVLNGGVNGVEMSAYASIDFLSHNLKEASAVKGLHAEDQVQEKTYAWKKALAGFNMVH

Target ORF information:

RefSeq Version	XM_015277882.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus insulin receptor substrate 2 (IRS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277882.2

ORF Insert Sequence:

ATGGCGAGCC CCGCCGCGCC GGGGCTGCCG CCCGGCCCCA ACCTGAACAA CAACAACAAC 
AACCGCGGCG TGAGGAAGTG CGGCTACCTG CGCAAGCAGA AGCACGGCCA CAAGCGCTTC 
TTCGTGCTGC GCGGCCCGGG CGGCGGCGGG GAGGAGGCGG GGGGCGCCCG GCTGGAGTAC 
TACGAGAGCG AGAAGAAATG GAGGAACAAG TCCGGGGCGC CCAAGCGGGT GATCGCGCTG 
GACTCGTGCC TCAACATCAA CAAGCGGGCG GACGCCAAGC ACAAGTACCT CATCGCCCTC 
TATACCAAGG ACGAGTACTT TGCCGTGGCG GCCGAGAACG AGCAGGAGCA GGAGGGCTGG 
TACCGCGCCC TCACCGACCT GCTGAGCGAG GGCAAGGCGG CCGCCGGCTC CCCGCACCGC 
CCCCTGCCCT CGCCCTTCGC CGCGCTGGCC GCCGGCGAGG AGCCCGACTA CGGGCTGATG 
ACGCCGGCCG CCGCCGCCTA CCGGGAGGTC TGGCAGGTGA CGCTGAAGCC CAAAGGCTTG 
GGGCAGAGCA AGAACCTCAC CGGCGTGCAC CGCCTCTGCC TCTCGGCCCG CACCATCGGC 
TTCGTGCGCC TCAACTGCGA GCTGCCCTCG GTCACGCTGC AGCTGATGAA CATCCGCCGC 
TGCGGCCACT CGGACAGCTT CTTCTTCATC GAGGTGGGCC GTTCGGCCGC CACCGGGCCC 
GGCGAGCTGT GGATGCAGGC GGACGACTCG GTGGTGGCCC AGAACATCCA CGAGACCATC 
CTGGAGGCTA TGAAGGCTCT AAAGGAGCTG TCCGAGTTCC GGCCCCGCAG CAAGAGCCAG 
TCGTCGTCCT CCTCTTCCTC CGGGGGGGCG GCCGGAGGCG GCGGCCCCTC GGCCACGCAC 
CCCATCACCG TGCCGGGCCG CCGGCACCAC CACCTGGTCA ACCTGCCGCC CAGCCAGACC 
GGCCTGCTCC GCCGATCCCG CACCGACAGC CTGGCCGCCG GCAGCAGGGC CGCGCCGTGC 
CGGGTGCGGA CGGCCAGCGA GGGCGACGGC TGCCGGGTGG GCTCGGCCGC CGGCAGCCCC 
ATGAGCCCCG GCCCCGTGCG GACCCCGCTC AGCCGCTCGC ACACGCTGAG CGGCGGCGGA 
GGGCGGCCGG CGGGCAAGCT GCTGCCCGTG CTGGCCGGGG GCGGGCTGCA AAGCAGCCGC 
TCCATGTCCA TGCCCGCCTC CCACTCGCCC CCCTCCGCCA CTAGCCCCGT CAGTCTCTCC 
TCCAGCAGCG GCCTGGGCTC CGAGCCGGCC CACCCGCATC ACCCGCAGCG CCCGTCCAGC 
GGCAGCGCCT CGGTGTCCGG CTCGCCCAGC GACGCGGGCT TCATGTCCTT CGACGAGTAC 
GGCTCCAGCC CGGGCGGCGA CCCGCGGCCC TTCTCCTCCT CCTCCACCGC CAGCAACCGC 
AGCAACACAC CCGAATCGGT GGCCGAGACC CCCCCGGTGC GGGACTCCGG CGGCGGCACC 
GATCTGTATG GCTACATGGC GATGGAGCGG CCCCCGAGCG GCCGTCTCTG CTACCGGCCT 
TGCCCCGAGG CTGACCGGGG CCACCGAAAG CGGACCTACT CGCTGACCAC CCCGTGCCGG 
CAGCGGCCCG CCCCGCCGCA GGTCTCCTCC GCCTCCCTGG ACGAGTACAC GCTAATGAGG 
GCCACCTTCG CCGGCAGCGC CGGCCGCCTC TTCCCCTCCT GCCCGGCAGG GGCTTCCCCT 
AAAGTCACCT ACACGCCGTA CCCCGAGGAC TACGGCGACA TCGAGATCGG CTCGCACCGC 
AGCTCCGGCA GCAGCAGCAC CAACCTGGGG CCGCCGGCAG GGGGGGGAGG CGGAGGCAGG 
GGCGGAGATG ACGATGGCTA CATGCCTATG ACCCCCGGCG TGGCCGCGGC CCTGGGTCGG 
GGGGGGCGGG GTGGCGATGA CTACATGCCC ATGAGCCCCA CCAGCGTGTC GGCCCCCAAA 
CAGATCCTGC AGCCCCGAGC GGGGGTGGGA GGCGGCTCCC CCGGCAACGG GAGCAGCTAT 
AAGACCAGCT CGCCGGGGGA AAGCTCCCCC GACGACAGCG GCTACATGAG GATGTGGTGC 
GGCTCTAAGC TGTCCGTGGA GAGTTCGGAC GGGAGGCTGA GCAACGGCGA CTACATCAAC 
ATGTCCCCTC GAGACCCCCA GCACGGGTCC CAGGCTCCCT CCCTCACCCC CCCGGACTTT 
TTCTTCGCCC CCGCAGGGCA CGGGGCTGGC GAGCCCCTCA AACCCGGCTG CTACTCTTAC 
AGCTCCCTGC CCCGCTCCTA CAAGAGCCAG GGCCCAGCTA AGGACAGCGA CCAGTACGTG 
TTCATGAACT CTCCGGGCAG GATGATCCCG GAGGAGGCGA TGTGCGGAGC GGTTCTGTCC 
CCAGCCGGCA CCTTCGCCCC CTCCAGCCAC ACGGTGCCTT CGCCCCTGCG GCACAGCCGG 
ACCGAGGGCT TCCTCAGCCA GCGGTGCCAG CGGGCCATCC GGCCCAGCCG CCTCTCTCTG 
GAGACCTTGC GGACGATGCT GCCAAGCATG AACGAGCACC CCTTGCCTCC CGAGCCCAAG 
AGCCCCGGGG AATACATCAA CATTGACTTC GGGGACACCG CTGTCTATTC TCCCCCCTCG 
CTGCCCACCG ACAGCCCAGC CTCCTCCCTG GGCTCAGGCA CAGGGCAGAG GCGCTCCCCT 
CTCTCCGACT ACATGAACAT CGACTTCAGC TCGCAGTCAC CCTCCCAGTC AGGCACAGTC 
TCGGTGGGCT CTTTGGAAGC GCTCTCGCCT GGCTCTTCCT CCAGCACCAG TCAGCCCGAT 
GGGCGCTACA TGAAGGCGGC TGGGGGGGTG GCCTGTCTGT CCAGCCCATC TGACAGCGGG 
GATTACACTG AGATGACCTT TGGCATGGCC ACTACCCCAC CCCAACCCAT CGTTCAGAAG 
CCAGAAAGTG CCCGGGTCAC CAGCCCCACG GCCGGGGTGA AGAGGCTCAC CCTCTCCGGG 
GTGGAGGCCT TCATTCTTTC CAGCCCACCC CCAGACCCCA ACCGCGGGGC CAAGGTCATT 
CGGGCTGACC CCCAGGGGCG CAGGAGGCAC AGCTCGGAAA CTTTCTCCTC CACCACCACT 
GTGACCCCCG TGTCCCCCTC CTTTGCACAC AACCCCAAAA GGCACAACTC AGCCTCGGTG 
GAGAACGTGT CCCTCAGGAA AAGCGAAGGC CTGGAGGAGG AGCAGGGCAG CAGCCCCATG 
TGCCGGGAGA CCTCGGCTGG CTTCCAGAAT GGCCTCAACT ACATCGCCGT CGACTTGGTG 
GACGGCAGCC TGGCAGGCTG TGACAAAGCC AGGTCAAAAG CCAGGCATGT CCTGAACGGA 
GGTGTCAACG GAGTGGAGAT GAGCGCCTAT GCCAGCATAG ACTTTCTGTC TCACAACCTG 
AAAGAAGCAA GCGCTGTGAA AGGGCTTCAT GCGGAAGACC AAGTCCAGGA GAAAACCTAT 
GCTTGGAAAA AGGCCTTGGC TGGCTTTAAC ATGGTCCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IRS2 ( XM_015277882.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133368.1 Gallus gallus uncharacterized LOC101749060 (LOC101749060), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa30699
Clone ID Related Accession (Same CDS sequence)	XM_015277882.2 , XM_015277882.1
Accession Version	XM_015277882.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	insulin receptor substrate 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763585.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCC CCGCCGCGCC GGGGCTGCCG CCCGGCCCCA ACCTGAACAA CAACAACAAC 
AACCGCGGCG TGAGGAAGTG CGGCTACCTG CGCAAGCAGA AGCACGGCCA CAAGCGCTTC 
TTCGTGCTGC GCGGCCCGGG CGGCGGCGGG GAGGAGGCGG GGGGCGCCCG GCTGGAGTAC 
TACGAGAGCG AGAAGAAATG GAGGAACAAG TCCGGGGCGC CCAAGCGGGT GATCGCGCTG 
GACTCGTGCC TCAACATCAA CAAGCGGGCG GACGCCAAGC ACAAGTACCT CATCGCCCTC 
TATACCAAGG ACGAGTACTT TGCCGTGGCG GCCGAGAACG AGCAGGAGCA GGAGGGCTGG 
TACCGCGCCC TCACCGACCT GCTGAGCGAG GGCAAGGCGG CCGCCGGCTC CCCGCACCGC 
CCCCTGCCCT CGCCCTTCGC CGCGCTGGCC GCCGGCGAGG AGCCCGACTA CGGGCTGATG 
ACGCCGGCCG CCGCCGCCTA CCGGGAGGTC TGGCAGGTGA CGCTGAAGCC CAAAGGCTTG 
GGGCAGAGCA AGAACCTCAC CGGCGTGCAC CGCCTCTGCC TCTCGGCCCG CACCATCGGC 
TTCGTGCGCC TCAACTGCGA GCTGCCCTCG GTCACGCTGC AGCTGATGAA CATCCGCCGC 
TGCGGCCACT CGGACAGCTT CTTCTTCATC GAGGTGGGCC GTTCGGCCGC CACCGGGCCC 
GGCGAGCTGT GGATGCAGGC GGACGACTCG GTGGTGGCCC AGAACATCCA CGAGACCATC 
CTGGAGGCTA TGAAGGCTCT AAAGGAGCTG TCCGAGTTCC GGCCCCGCAG CAAGAGCCAG 
TCGTCGTCCT CCTCTTCCTC CGGGGGGGCG GCCGGAGGCG GCGGCCCCTC GGCCACGCAC 
CCCATCACCG TGCCGGGCCG CCGGCACCAC CACCTGGTCA ACCTGCCGCC CAGCCAGACC 
GGCCTGCTCC GCCGATCCCG CACCGACAGC CTGGCCGCCG GCAGCAGGGC CGCGCCGTGC 
CGGGTGCGGA CGGCCAGCGA GGGCGACGGC TGCCGGGTGG GCTCGGCCGC CGGCAGCCCC 
ATGAGCCCCG GCCCCGTGCG GACCCCGCTC AGCCGCTCGC ACACGCTGAG CGGCGGCGGA 
GGGCGGCCGG CGGGCAAGCT GCTGCCCGTG CTGGCCGGGG GCGGGCTGCA AAGCAGCCGC 
TCCATGTCCA TGCCCGCCTC CCACTCGCCC CCCTCCGCCA CTAGCCCCGT CAGTCTCTCC 
TCCAGCAGCG GCCTGGGCTC CGAGCCGGCC CACCCGCATC ACCCGCAGCG CCCGTCCAGC 
GGCAGCGCCT CGGTGTCCGG CTCGCCCAGC GACGCGGGCT TCATGTCCTT CGACGAGTAC 
GGCTCCAGCC CGGGCGGCGA CCCGCGGCCC TTCTCCTCCT CCTCCACCGC CAGCAACCGC 
AGCAACACAC CCGAATCGGT GGCCGAGACC CCCCCGGTGC GGGACTCCGG CGGCGGCACC 
GATCTGTATG GCTACATGGC GATGGAGCGG CCCCCGAGCG GCCGTCTCTG CTACCGGCCT 
TGCCCCGAGG CTGACCGGGG CCACCGAAAG CGGACCTACT CGCTGACCAC CCCGTGCCGG 
CAGCGGCCCG CCCCGCCGCA GGTCTCCTCC GCCTCCCTGG ACGAGTACAC GCTAATGAGG 
GCCACCTTCG CCGGCAGCGC CGGCCGCCTC TTCCCCTCCT GCCCGGCAGG GGCTTCCCCT 
AAAGTCACCT ACACGCCGTA CCCCGAGGAC TACGGCGACA TCGAGATCGG CTCGCACCGC 
AGCTCCGGCA GCAGCAGCAC CAACCTGGGG CCGCCGGCAG GGGGGGGAGG CGGAGGCAGG 
GGCGGAGATG ACGATGGCTA CATGCCTATG ACCCCCGGCG TGGCCGCGGC CCTGGGTCGG 
GGGGGGCGGG GTGGCGATGA CTACATGCCC ATGAGCCCCA CCAGCGTGTC GGCCCCCAAA 
CAGATCCTGC AGCCCCGAGC GGGGGTGGGA GGCGGCTCCC CCGGCAACGG GAGCAGCTAT 
AAGACCAGCT CGCCGGGGGA AAGCTCCCCC GACGACAGCG GCTACATGAG GATGTGGTGC 
GGCTCTAAGC TGTCCGTGGA GAGTTCGGAC GGGAGGCTGA GCAACGGCGA CTACATCAAC 
ATGTCCCCTC GAGACCCCCA GCACGGGTCC CAGGCTCCCT CCCTCACCCC CCCGGACTTT 
TTCTTCGCCC CCGCAGGGCA CGGGGCTGGC GAGCCCCTCA AACCCGGCTG CTACTCTTAC 
AGCTCCCTGC CCCGCTCCTA CAAGAGCCAG GGCCCAGCTA AGGACAGCGA CCAGTACGTG 
TTCATGAACT CTCCGGGCAG GATGATCCCG GAGGAGGCGA TGTGCGGAGC GGTTCTGTCC 
CCAGCCGGCA CCTTCGCCCC CTCCAGCCAC ACGGTGCCTT CGCCCCTGCG GCACAGCCGG 
ACCGAGGGCT TCCTCAGCCA GCGGTGCCAG CGGGCCATCC GGCCCAGCCG CCTCTCTCTG 
GAGACCTTGC GGACGATGCT GCCAAGCATG AACGAGCACC CCTTGCCTCC CGAGCCCAAG 
AGCCCCGGGG AATACATCAA CATTGACTTC GGGGACACCG CTGTCTATTC TCCCCCCTCG 
CTGCCCACCG ACAGCCCAGC CTCCTCCCTG GGCTCAGGCA CAGGGCAGAG GCGCTCCCCT 
CTCTCCGACT ACATGAACAT CGACTTCAGC TCGCAGTCAC CCTCCCAGTC AGGCACAGTC 
TCGGTGGGCT CTTTGGAAGC GCTCTCGCCT GGCTCTTCCT CCAGCACCAG TCAGCCCGAT 
GGGCGCTACA TGAAGGCGGC TGGGGGGGTG GCCTGTCTGT CCAGCCCATC TGACAGCGGG 
GATTACACTG AGATGACCTT TGGCATGGCC ACTACCCCAC CCCAACCCAT CGTTCAGAAG 
CCAGAAAGTG CCCGGGTCAC CAGCCCCACG GCCGGGGTGA AGAGGCTCAC CCTCTCCGGG 
GTGGAGGCCT TCATTCTTTC CAGCCCACCC CCAGACCCCA ACCGCGGGGC CAAGGTCATT 
CGGGCTGACC CCCAGGGGCG CAGGAGGCAC AGCTCGGAAA CTTTCTCCTC CACCACCACT 
GTGACCCCCG TGTCCCCCTC CTTTGCACAC AACCCCAAAA GGCACAACTC AGCCTCGGTG 
GAGAACGTGT CCCTCAGGAA AAGCGAAGGC CTGGAGGAGG AGCAGGGCAG CAGCCCCATG 
TGCCGGGAGA CCTCGGCTGG CTTCCAGAAT GGCCTCAACT ACATCGCCGT CGACTTGGTG 
GACGGCAGCC TGGCAGGCTG TGACAAAGCC AGGTCAAAAG CCAGGCATGT CCTGAACGGA 
GGTGTCAACG GAGTGGAGAT GAGCGCCTAT GCCAGCATAG ACTTTCTGTC TCACAACCTG 
AAAGAAGCAA GCGCTGTGAA AGGGCTTCAT GCGGAAGACC AAGTCCAGGA GAAAACCTAT 
GCTTGGAAAA AGGCCTTGGC TGGCTTTAAC ATGGTCCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133368.1
CDS	126..3647
Translation	MASPAAPGLPPGPNLNNNNNNRGVRKCGYLRKQKHGHKRFFVLRGPGGGGEEAGGARLEYYESEKKWRNKSGAPKRVIALDSCLNINKRADAKHKYLIALYTKDEYFAVAAENEQEQEGWYRALTDLLSEGKAAAGSPHRPLPSPFAALAAGEEPDYGLMTPAAAAYREVWQVTLKPKGLGQSKNLTGVHRLCLSARTIGFVRLNCELPSVTLQLMNIRRCGHSDSFFFIEVGRSAATGPGELWMQADDSVVAQNIHETILEAMKALKELSEFRPRSKSQSSSSSSSGGAAGGGGPSATHPITVPGRRHHHLVNLPPSQTGLLRRSRTDSLAAGSRAAPCRVRTASEGDGCRVGSAAGSPMSPGPVRTPLSRSHTLSGGGGRPAGKLLPVLAGGGLQSSRSMSMPASHSPPSATSPVSLSSSSGLGSEPAHPHHPQRPSSGSASVSGSPSDAGFMSFDEYGSSPGGDPRPFSSSSTASNRSNTPESVAETPPVRDSGGGTDLYGYMAMERPPSGRLCYRPCPEADRGHRKRTYSLTTPCRQRPAPPQVSSASLDEYTLMRATFAGSAGRLFPSCPAGASPKVTYTPYPEDYGDIEIGSHRSSGSSSTNLGPPAGGGGGGRGGDDDGYMPMTPGVAAALGRGGRGGDDYMPMSPTSVSAPKQILQPRAGVGGGSPGNGSSYKTSSPGESSPDDSGYMRMWCGSKLSVESSDGRLSNGDYINMSPRDPQHGSQAPSLTPPDFFFAPAGHGAGEPLKPGCYSYSSLPRSYKSQGPAKDSDQYVFMNSPGRMIPEEAMCGAVLSPAGTFAPSSHTVPSPLRHSRTEGFLSQRCQRAIRPSRLSLETLRTMLPSMNEHPLPPEPKSPGEYINIDFGDTAVYSPPSLPTDSPASSLGSGTGQRRSPLSDYMNIDFSSQSPSQSGTVSVGSLEALSPGSSSSTSQPDGRYMKAAGGVACLSSPSDSGDYTEMTFGMATTPPQPIVQKPESARVTSPTAGVKRLTLSGVEAFILSSPPPDPNRGAKVIRADPQGRRRHSSETFSSTTTVTPVSPSFAHNPKRHNSASVENVSLRKSEGLEEEQGSSPMCRETSAGFQNGLNYIAVDLVDGSLAGCDKARSKARHVLNGGVNGVEMSAYASIDFLSHNLKEASAVKGLHAEDQVQEKTYAWKKALAGFNMVH

Target ORF information:

RefSeq Version	XM_015277882.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus uncharacterized LOC101749060 (LOC101749060), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277882.1

ORF Insert Sequence:

ATGGCGAGCC CCGCCGCGCC GGGGCTGCCG CCCGGCCCCA ACCTGAACAA CAACAACAAC 
AACCGCGGCG TGAGGAAGTG CGGCTACCTG CGCAAGCAGA AGCACGGCCA CAAGCGCTTC 
TTCGTGCTGC GCGGCCCGGG CGGCGGCGGG GAGGAGGCGG GGGGCGCCCG GCTGGAGTAC 
TACGAGAGCG AGAAGAAATG GAGGAACAAG TCCGGGGCGC CCAAGCGGGT GATCGCGCTG 
GACTCGTGCC TCAACATCAA CAAGCGGGCG GACGCCAAGC ACAAGTACCT CATCGCCCTC 
TATACCAAGG ACGAGTACTT TGCCGTGGCG GCCGAGAACG AGCAGGAGCA GGAGGGCTGG 
TACCGCGCCC TCACCGACCT GCTGAGCGAG GGCAAGGCGG CCGCCGGCTC CCCGCACCGC 
CCCCTGCCCT CGCCCTTCGC CGCGCTGGCC GCCGGCGAGG AGCCCGACTA CGGGCTGATG 
ACGCCGGCCG CCGCCGCCTA CCGGGAGGTC TGGCAGGTGA CGCTGAAGCC CAAAGGCTTG 
GGGCAGAGCA AGAACCTCAC CGGCGTGCAC CGCCTCTGCC TCTCGGCCCG CACCATCGGC 
TTCGTGCGCC TCAACTGCGA GCTGCCCTCG GTCACGCTGC AGCTGATGAA CATCCGCCGC 
TGCGGCCACT CGGACAGCTT CTTCTTCATC GAGGTGGGCC GTTCGGCCGC CACCGGGCCC 
GGCGAGCTGT GGATGCAGGC GGACGACTCG GTGGTGGCCC AGAACATCCA CGAGACCATC 
CTGGAGGCTA TGAAGGCTCT AAAGGAGCTG TCCGAGTTCC GGCCCCGCAG CAAGAGCCAG 
TCGTCGTCCT CCTCTTCCTC CGGGGGGGCG GCCGGAGGCG GCGGCCCCTC GGCCACGCAC 
CCCATCACCG TGCCGGGCCG CCGGCACCAC CACCTGGTCA ACCTGCCGCC CAGCCAGACC 
GGCCTGCTCC GCCGATCCCG CACCGACAGC CTGGCCGCCG GCAGCAGGGC CGCGCCGTGC 
CGGGTGCGGA CGGCCAGCGA GGGCGACGGC TGCCGGGTGG GCTCGGCCGC CGGCAGCCCC 
ATGAGCCCCG GCCCCGTGCG GACCCCGCTC AGCCGCTCGC ACACGCTGAG CGGCGGCGGA 
GGGCGGCCGG CGGGCAAGCT GCTGCCCGTG CTGGCCGGGG GCGGGCTGCA AAGCAGCCGC 
TCCATGTCCA TGCCCGCCTC CCACTCGCCC CCCTCCGCCA CTAGCCCCGT CAGTCTCTCC 
TCCAGCAGCG GCCTGGGCTC CGAGCCGGCC CACCCGCATC ACCCGCAGCG CCCGTCCAGC 
GGCAGCGCCT CGGTGTCCGG CTCGCCCAGC GACGCGGGCT TCATGTCCTT CGACGAGTAC 
GGCTCCAGCC CGGGCGGCGA CCCGCGGCCC TTCTCCTCCT CCTCCACCGC CAGCAACCGC 
AGCAACACAC CCGAATCGGT GGCCGAGACC CCCCCGGTGC GGGACTCCGG CGGCGGCACC 
GATCTGTATG GCTACATGGC GATGGAGCGG CCCCCGAGCG GCCGTCTCTG CTACCGGCCT 
TGCCCCGAGG CTGACCGGGG CCACCGAAAG CGGACCTACT CGCTGACCAC CCCGTGCCGG 
CAGCGGCCCG CCCCGCCGCA GGTCTCCTCC GCCTCCCTGG ACGAGTACAC GCTAATGAGG 
GCCACCTTCG CCGGCAGCGC CGGCCGCCTC TTCCCCTCCT GCCCGGCAGG GGCTTCCCCT 
AAAGTCACCT ACACGCCGTA CCCCGAGGAC TACGGCGACA TCGAGATCGG CTCGCACCGC 
AGCTCCGGCA GCAGCAGCAC CAACCTGGGG CCGCCGGCAG GGGGGGGAGG CGGAGGCAGG 
GGCGGAGATG ACGATGGCTA CATGCCTATG ACCCCCGGCG TGGCCGCGGC CCTGGGTCGG 
GGGGGGCGGG GTGGCGATGA CTACATGCCC ATGAGCCCCA CCAGCGTGTC GGCCCCCAAA 
CAGATCCTGC AGCCCCGAGC GGGGGTGGGA GGCGGCTCCC CCGGCAACGG GAGCAGCTAT 
AAGACCAGCT CGCCGGGGGA AAGCTCCCCC GACGACAGCG GCTACATGAG GATGTGGTGC 
GGCTCTAAGC TGTCCGTGGA GAGTTCGGAC GGGAGGCTGA GCAACGGCGA CTACATCAAC 
ATGTCCCCTC GAGACCCCCA GCACGGGTCC CAGGCTCCCT CCCTCACCCC CCCGGACTTT 
TTCTTCGCCC CCGCAGGGCA CGGGGCTGGC GAGCCCCTCA AACCCGGCTG CTACTCTTAC 
AGCTCCCTGC CCCGCTCCTA CAAGAGCCAG GGCCCAGCTA AGGACAGCGA CCAGTACGTG 
TTCATGAACT CTCCGGGCAG GATGATCCCG GAGGAGGCGA TGTGCGGAGC GGTTCTGTCC 
CCAGCCGGCA CCTTCGCCCC CTCCAGCCAC ACGGTGCCTT CGCCCCTGCG GCACAGCCGG 
ACCGAGGGCT TCCTCAGCCA GCGGTGCCAG CGGGCCATCC GGCCCAGCCG CCTCTCTCTG 
GAGACCTTGC GGACGATGCT GCCAAGCATG AACGAGCACC CCTTGCCTCC CGAGCCCAAG 
AGCCCCGGGG AATACATCAA CATTGACTTC GGGGACACCG CTGTCTATTC TCCCCCCTCG 
CTGCCCACCG ACAGCCCAGC CTCCTCCCTG GGCTCAGGCA CAGGGCAGAG GCGCTCCCCT 
CTCTCCGACT ACATGAACAT CGACTTCAGC TCGCAGTCAC CCTCCCAGTC AGGCACAGTC 
TCGGTGGGCT CTTTGGAAGC GCTCTCGCCT GGCTCTTCCT CCAGCACCAG TCAGCCCGAT 
GGGCGCTACA TGAAGGCGGC TGGGGGGGTG GCCTGTCTGT CCAGCCCATC TGACAGCGGG 
GATTACACTG AGATGACCTT TGGCATGGCC ACTACCCCAC CCCAACCCAT CGTTCAGAAG 
CCAGAAAGTG CCCGGGTCAC CAGCCCCACG GCCGGGGTGA AGAGGCTCAC CCTCTCCGGG 
GTGGAGGCCT TCATTCTTTC CAGCCCACCC CCAGACCCCA ACCGCGGGGC CAAGGTCATT 
CGGGCTGACC CCCAGGGGCG CAGGAGGCAC AGCTCGGAAA CTTTCTCCTC CACCACCACT 
GTGACCCCCG TGTCCCCCTC CTTTGCACAC AACCCCAAAA GGCACAACTC AGCCTCGGTG 
GAGAACGTGT CCCTCAGGAA AAGCGAAGGC CTGGAGGAGG AGCAGGGCAG CAGCCCCATG 
TGCCGGGAGA CCTCGGCTGG CTTCCAGAAT GGCCTCAACT ACATCGCCGT CGACTTGGTG 
GACGGCAGCC TGGCAGGCTG TGACAAAGCC AGGTCAAAAG CCAGGCATGT CCTGAACGGA 
GGTGTCAACG GAGTGGAGAT GAGCGCCTAT GCCAGCATAG ACTTTCTGTC TCACAACCTG 
AAAGAAGCAA GCGCTGTGAA AGGGCTTCAT GCGGAAGACC AAGTCCAGGA GAAAACCTAT 
GCTTGGAAAA AGGCCTTGGC TGGCTTTAAC ATGGTCCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IRS2 ( XM_015277883.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133369.1 Gallus gallus insulin receptor substrate 2 (IRS2), transcript variant X2, mRNA.

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CloneID	OGa30700
Clone ID Related Accession (Same CDS sequence)	XM_015277883.2 , XM_015277883.1
Accession Version	XM_015277883.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	insulin receptor substrate 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277883.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCC CCGCCGCGCC GGGGCTGCCG CCCGGCCCCA ACCTGAACAA CAACAACAAC 
AACCGCGGCG TGAGGAAGTG CGGCTACCTG CGCAAGCAGA AGCACGGCCA CAAGCGCTTC 
TTCGTGCTGC GCGGCCCGGG CGGCGGCGGG GAGGAGGCGG GGGGCGCCCG GCTGGAGTAC 
TACGAGAGCG AGAAGAAATG GAGGAACAAG TCCGGGGCGC CCAAGCGGGT GATCGCGCTG 
GACTCGTGCC TCAACATCAA CAAGCGGGCG GACGCCAAGC ACAAGTACCT CATCGCCCTC 
TATACCAAGG ACGAGTACTT TGCCGTGGCG GCCGAGAACG AGCAGGAGCA GGAGGGCTGG 
TACCGCGCCC TCACCGACCT GCTGAGCGAG GGCAAGGCGG CCGCCGGCTC CCCGCACCGC 
CCCCTGCCCT CGCCCTTCGC CGCGCTGGCC GCCGGCGAGG AGCCCGACTA CGGGCTGATG 
ACGCCGGCCG CCGCCGCCTA CCGGGAGGTC TGGCAGGTGA CGCTGAAGCC CAAAGGCTTG 
GGGCAGAGCA AGAACCTCAC CGGCGTGCAC CGCCTCTGCC TCTCGGCCCG CACCATCGGC 
TTCGTGCGCC TCAACTGCGA GCTGCCCTCG GTCACGCTGC AGCTGATGAA CATCCGCCGC 
TGCGGCCACT CGGACAGCTT CTTCTTCATC GAGGTGGGCC GTTCGGCCGC CACCGGGCCC 
GGCGAGCTGT GGATGCAGGC GGACGACTCG GTGGTGGCCC AGAACATCCA CGAGACCATC 
CTGGAGGCTA TGAAGGCTCT AAAGGAGCTG TCCGAGTTCC GGCCCCGCAG CAAGAGCCAG 
TCGTCGTCCT CCTCTTCCTC CGGGGGGGCG GCCGGAGGCG GCGGCCCCTC GGCCACGCAC 
CCCATCACCG TGCCGGGCCG CCGGCACCAC CACCTGGTCA ACCTGCCGCC CAGCCAGACC 
GGCCTGCTCC GCCGATCCCG CACCGACAGC CTGGCCGCCG GCAGCAGGGC CGCGCCGTGC 
CGGGTGCGGA CGGCCAGCGA GGGCGACGGC TGCCGGGTGG GCTCGGCCGC CGGCAGCCCC 
ATGAGCCCCG GCCCCGTGCG GACCCCGCTC AGCCGCTCGC ACACGCTGAG CGGCGGCGGA 
GGGCGGCCGG CGGGCAAGCT GCTGCCCGTG CTGGCCGGGG GCGGGCTGCA AAGCAGCCGC 
TCCATGTCCA TGCCCGCCTC CCACTCGCCC CCCTCCGCCA CTAGCCCCGT CAGTCTCTCC 
TCCAGCAGCG GCCTGGGCTC CGAGCCGGCC CACCCGCATC ACCCGCAGCG CCCGTCCAGC 
GGCAGCGCCT CGGTGTCCGG CTCGCCCAGC GACGCGGGCT TCATGTCCTT CGACGAGTAC 
GGCTCCAGCC CGGGCGGCGA CCCGCGGCCC TTCTCCTCCT CCTCCACCGC CAGCAACCGC 
AGCAACACAC CCGAATCGGT GGCCGAGACC CCCCCGGTGC GGGACTCCGG CGGCGGCACC 
GATCTGTATG GCTACATGGC GATGGAGCGG CCCCCGAGCG GCCGTCTCTG CTACCGGCCT 
TGCCCCGAGG CTGACCGGGG CCACCGAAAG CGGACCTACT CGCTGACCAC CCCGTGCCGG 
CAGCGGCCCG CCCCGCCGCA GGTCTCCTCC GCCTCCCTGG ACGAGTACAC GCTAATGAGG 
GCCACCTTCG CCGGCAGCGC CGGCCGCCTC TTCCCCTCCT GCCCGGCAGG GGCTTCCCCT 
AAAGTCACCT ACACGCCGTA CCCCGAGGAC TACGGCGACA TCGAGATCGG CTCGCACCGC 
AGCTCCGGCA GCAGCAGCAC CAACCTGGGG CCGCCGGCAG GGGGGGGAGG CGGAGGCAGG 
GGCGGAGATG ACGATGGCTA CATGCCTATG ACCCCCGGCG TGGCCGCGGC CCTGGGTCGG 
GGGGGGCGGG GTGGCGATGA CTACATGCCC ATGAGCCCCA CCAGCGTGTC GGCCCCCAAA 
CAGATCCTGC AGCCCCGAGC GGGGGTGGGA GGCGGCTCCC CCGGCAACGG GAGCAGCTAT 
AAGACCAGCT CGCCGGGGGA AAGCTCCCCC GACGACAGCG GCTACATGAG GATGTGGTGC 
GGCTCTAAGC TGTCCGTGGA GAGTTCGGAC GGGAGGCTGA GCAACGGCGA CTACATCAAC 
ATGTCCCCTC GAGACCCCCA GCACGGGTCC CAGGCTCCCT CCCTCACCCC CCCGGACTTT 
TTCTTCGCCC CCGCAGGGCA CGGGGCTGGC GAGCCCCTCA AACCCGGCTG CTACTCTTAC 
AGCTCCCTGC CCCGCTCCTA CAAGAGCCAG GGCCCAGCTA AGGACAGCGA CCAGTACGTG 
TTCATGAACT CTCCGGGCAG GATGATCCCG GAGGAGGCGA TGTGCGGAGC GGTTCTGTCC 
CCAGCCGGCA CCTTCGCCCC CTCCAGCCAC ACGGTGCCTT CGCCCCTGCG GCACAGCCGG 
ACCGAGGGCT TCCTCAGCCA GCGGTGCCAG CGGGCCATCC GGCCCAGCCG CCTCTCTCTG 
GAGACCTTGC GGACGATGCT GCCAAGCATG AACGAGCACC CCTTGCCTCC CGAGCCCAAG 
AGCCCCGGGG AATACATCAA CATTGACTTC GGGGACACCG CTGTCTATTC TCCCCCCTCG 
CTGCCCACCG ACAGCCCAGC CTCCTCCCTG GGCTCAGGCA CAGGGCAGAG GCGCTCCCCT 
CTCTCCGACT ACATGAACAT CGACTTCAGC TCGCAGTCAC CCTCCCAGTC AGGCACAGTC 
TCGGTGGGCT CTTTGGAAGC GCTCTCGCCT GGCTCTTCCT CCAGCACCAG TCAGCCCGAT 
GGGCGCTACA TGAAGGCGGC TGGGGGGGTG GCCTGTCTGT CCAGCCCATC TGACAGCGGG 
GATTACACTG AGATGACCTT TGGCATGGCC ACTACCCCAC CCCAACCCAT CGTTCAGAAG 
CCAGAAAGTG CCCGGGTCAC CAGCCCCACG GCCGGGGTGA AGAGGCTCAC CCTCTCCGGG 
GTGGAGGCCT TCATTCTTTC CAGCCCACCC CCAGACCCCA ACCGCGGGGC CAAGGTCATT 
CGGGCTGACC CCCAGGGGCG CAGGAGGCAC AGCTCGGAAA CTTTCTCCTC CACCACCACT 
GTGACCCCCG TGTCCCCCTC CTTTGCACAC AACCCCAAAA GGCACAACTC AGCCTCGGTG 
GAGAACGTGT CCCTCAGGAA AAGCGAAGGC CTGGAGGAGG AGCAGGGCAG CAGCCCCATG 
TGCCGGGAGA CCTCGGCTGG CTTCCAGAAT GGCCTCAACT ACATCGCCGT CGACTTGGTG 
GACGGCAGCC TGGCAGGCTG TGACAAAGCC AGGTCAAAAG CCAGGCATGT CCTGAACGGA 
GGTGTCAACG GAGTGGAGAT GAGCGCCTAT GCCAGCATAG ACTTTCTGTC TCACAACCTG 
AAAGAAGCAA GCGCTGTGAA AGGAAGTACA GGAAGATGGA AAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133369.1
CDS	187..3654
Translation	MASPAAPGLPPGPNLNNNNNNRGVRKCGYLRKQKHGHKRFFVLRGPGGGGEEAGGARLEYYESEKKWRNKSGAPKRVIALDSCLNINKRADAKHKYLIALYTKDEYFAVAAENEQEQEGWYRALTDLLSEGKAAAGSPHRPLPSPFAALAAGEEPDYGLMTPAAAAYREVWQVTLKPKGLGQSKNLTGVHRLCLSARTIGFVRLNCELPSVTLQLMNIRRCGHSDSFFFIEVGRSAATGPGELWMQADDSVVAQNIHETILEAMKALKELSEFRPRSKSQSSSSSSSGGAAGGGGPSATHPITVPGRRHHHLVNLPPSQTGLLRRSRTDSLAAGSRAAPCRVRTASEGDGCRVGSAAGSPMSPGPVRTPLSRSHTLSGGGGRPAGKLLPVLAGGGLQSSRSMSMPASHSPPSATSPVSLSSSSGLGSEPAHPHHPQRPSSGSASVSGSPSDAGFMSFDEYGSSPGGDPRPFSSSSTASNRSNTPESVAETPPVRDSGGGTDLYGYMAMERPPSGRLCYRPCPEADRGHRKRTYSLTTPCRQRPAPPQVSSASLDEYTLMRATFAGSAGRLFPSCPAGASPKVTYTPYPEDYGDIEIGSHRSSGSSSTNLGPPAGGGGGGRGGDDDGYMPMTPGVAAALGRGGRGGDDYMPMSPTSVSAPKQILQPRAGVGGGSPGNGSSYKTSSPGESSPDDSGYMRMWCGSKLSVESSDGRLSNGDYINMSPRDPQHGSQAPSLTPPDFFFAPAGHGAGEPLKPGCYSYSSLPRSYKSQGPAKDSDQYVFMNSPGRMIPEEAMCGAVLSPAGTFAPSSHTVPSPLRHSRTEGFLSQRCQRAIRPSRLSLETLRTMLPSMNEHPLPPEPKSPGEYINIDFGDTAVYSPPSLPTDSPASSLGSGTGQRRSPLSDYMNIDFSSQSPSQSGTVSVGSLEALSPGSSSSTSQPDGRYMKAAGGVACLSSPSDSGDYTEMTFGMATTPPQPIVQKPESARVTSPTAGVKRLTLSGVEAFILSSPPPDPNRGAKVIRADPQGRRRHSSETFSSTTTVTPVSPSFAHNPKRHNSASVENVSLRKSEGLEEEQGSSPMCRETSAGFQNGLNYIAVDLVDGSLAGCDKARSKARHVLNGGVNGVEMSAYASIDFLSHNLKEASAVKGSTGRWKR

Target ORF information:

RefSeq Version	XM_015277883.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus insulin receptor substrate 2 (IRS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277883.2

ORF Insert Sequence:

ATGGCGAGCC CCGCCGCGCC GGGGCTGCCG CCCGGCCCCA ACCTGAACAA CAACAACAAC 
AACCGCGGCG TGAGGAAGTG CGGCTACCTG CGCAAGCAGA AGCACGGCCA CAAGCGCTTC 
TTCGTGCTGC GCGGCCCGGG CGGCGGCGGG GAGGAGGCGG GGGGCGCCCG GCTGGAGTAC 
TACGAGAGCG AGAAGAAATG GAGGAACAAG TCCGGGGCGC CCAAGCGGGT GATCGCGCTG 
GACTCGTGCC TCAACATCAA CAAGCGGGCG GACGCCAAGC ACAAGTACCT CATCGCCCTC 
TATACCAAGG ACGAGTACTT TGCCGTGGCG GCCGAGAACG AGCAGGAGCA GGAGGGCTGG 
TACCGCGCCC TCACCGACCT GCTGAGCGAG GGCAAGGCGG CCGCCGGCTC CCCGCACCGC 
CCCCTGCCCT CGCCCTTCGC CGCGCTGGCC GCCGGCGAGG AGCCCGACTA CGGGCTGATG 
ACGCCGGCCG CCGCCGCCTA CCGGGAGGTC TGGCAGGTGA CGCTGAAGCC CAAAGGCTTG 
GGGCAGAGCA AGAACCTCAC CGGCGTGCAC CGCCTCTGCC TCTCGGCCCG CACCATCGGC 
TTCGTGCGCC TCAACTGCGA GCTGCCCTCG GTCACGCTGC AGCTGATGAA CATCCGCCGC 
TGCGGCCACT CGGACAGCTT CTTCTTCATC GAGGTGGGCC GTTCGGCCGC CACCGGGCCC 
GGCGAGCTGT GGATGCAGGC GGACGACTCG GTGGTGGCCC AGAACATCCA CGAGACCATC 
CTGGAGGCTA TGAAGGCTCT AAAGGAGCTG TCCGAGTTCC GGCCCCGCAG CAAGAGCCAG 
TCGTCGTCCT CCTCTTCCTC CGGGGGGGCG GCCGGAGGCG GCGGCCCCTC GGCCACGCAC 
CCCATCACCG TGCCGGGCCG CCGGCACCAC CACCTGGTCA ACCTGCCGCC CAGCCAGACC 
GGCCTGCTCC GCCGATCCCG CACCGACAGC CTGGCCGCCG GCAGCAGGGC CGCGCCGTGC 
CGGGTGCGGA CGGCCAGCGA GGGCGACGGC TGCCGGGTGG GCTCGGCCGC CGGCAGCCCC 
ATGAGCCCCG GCCCCGTGCG GACCCCGCTC AGCCGCTCGC ACACGCTGAG CGGCGGCGGA 
GGGCGGCCGG CGGGCAAGCT GCTGCCCGTG CTGGCCGGGG GCGGGCTGCA AAGCAGCCGC 
TCCATGTCCA TGCCCGCCTC CCACTCGCCC CCCTCCGCCA CTAGCCCCGT CAGTCTCTCC 
TCCAGCAGCG GCCTGGGCTC CGAGCCGGCC CACCCGCATC ACCCGCAGCG CCCGTCCAGC 
GGCAGCGCCT CGGTGTCCGG CTCGCCCAGC GACGCGGGCT TCATGTCCTT CGACGAGTAC 
GGCTCCAGCC CGGGCGGCGA CCCGCGGCCC TTCTCCTCCT CCTCCACCGC CAGCAACCGC 
AGCAACACAC CCGAATCGGT GGCCGAGACC CCCCCGGTGC GGGACTCCGG CGGCGGCACC 
GATCTGTATG GCTACATGGC GATGGAGCGG CCCCCGAGCG GCCGTCTCTG CTACCGGCCT 
TGCCCCGAGG CTGACCGGGG CCACCGAAAG CGGACCTACT CGCTGACCAC CCCGTGCCGG 
CAGCGGCCCG CCCCGCCGCA GGTCTCCTCC GCCTCCCTGG ACGAGTACAC GCTAATGAGG 
GCCACCTTCG CCGGCAGCGC CGGCCGCCTC TTCCCCTCCT GCCCGGCAGG GGCTTCCCCT 
AAAGTCACCT ACACGCCGTA CCCCGAGGAC TACGGCGACA TCGAGATCGG CTCGCACCGC 
AGCTCCGGCA GCAGCAGCAC CAACCTGGGG CCGCCGGCAG GGGGGGGAGG CGGAGGCAGG 
GGCGGAGATG ACGATGGCTA CATGCCTATG ACCCCCGGCG TGGCCGCGGC CCTGGGTCGG 
GGGGGGCGGG GTGGCGATGA CTACATGCCC ATGAGCCCCA CCAGCGTGTC GGCCCCCAAA 
CAGATCCTGC AGCCCCGAGC GGGGGTGGGA GGCGGCTCCC CCGGCAACGG GAGCAGCTAT 
AAGACCAGCT CGCCGGGGGA AAGCTCCCCC GACGACAGCG GCTACATGAG GATGTGGTGC 
GGCTCTAAGC TGTCCGTGGA GAGTTCGGAC GGGAGGCTGA GCAACGGCGA CTACATCAAC 
ATGTCCCCTC GAGACCCCCA GCACGGGTCC CAGGCTCCCT CCCTCACCCC CCCGGACTTT 
TTCTTCGCCC CCGCAGGGCA CGGGGCTGGC GAGCCCCTCA AACCCGGCTG CTACTCTTAC 
AGCTCCCTGC CCCGCTCCTA CAAGAGCCAG GGCCCAGCTA AGGACAGCGA CCAGTACGTG 
TTCATGAACT CTCCGGGCAG GATGATCCCG GAGGAGGCGA TGTGCGGAGC GGTTCTGTCC 
CCAGCCGGCA CCTTCGCCCC CTCCAGCCAC ACGGTGCCTT CGCCCCTGCG GCACAGCCGG 
ACCGAGGGCT TCCTCAGCCA GCGGTGCCAG CGGGCCATCC GGCCCAGCCG CCTCTCTCTG 
GAGACCTTGC GGACGATGCT GCCAAGCATG AACGAGCACC CCTTGCCTCC CGAGCCCAAG 
AGCCCCGGGG AATACATCAA CATTGACTTC GGGGACACCG CTGTCTATTC TCCCCCCTCG 
CTGCCCACCG ACAGCCCAGC CTCCTCCCTG GGCTCAGGCA CAGGGCAGAG GCGCTCCCCT 
CTCTCCGACT ACATGAACAT CGACTTCAGC TCGCAGTCAC CCTCCCAGTC AGGCACAGTC 
TCGGTGGGCT CTTTGGAAGC GCTCTCGCCT GGCTCTTCCT CCAGCACCAG TCAGCCCGAT 
GGGCGCTACA TGAAGGCGGC TGGGGGGGTG GCCTGTCTGT CCAGCCCATC TGACAGCGGG 
GATTACACTG AGATGACCTT TGGCATGGCC ACTACCCCAC CCCAACCCAT CGTTCAGAAG 
CCAGAAAGTG CCCGGGTCAC CAGCCCCACG GCCGGGGTGA AGAGGCTCAC CCTCTCCGGG 
GTGGAGGCCT TCATTCTTTC CAGCCCACCC CCAGACCCCA ACCGCGGGGC CAAGGTCATT 
CGGGCTGACC CCCAGGGGCG CAGGAGGCAC AGCTCGGAAA CTTTCTCCTC CACCACCACT 
GTGACCCCCG TGTCCCCCTC CTTTGCACAC AACCCCAAAA GGCACAACTC AGCCTCGGTG 
GAGAACGTGT CCCTCAGGAA AAGCGAAGGC CTGGAGGAGG AGCAGGGCAG CAGCCCCATG 
TGCCGGGAGA CCTCGGCTGG CTTCCAGAAT GGCCTCAACT ACATCGCCGT CGACTTGGTG 
GACGGCAGCC TGGCAGGCTG TGACAAAGCC AGGTCAAAAG CCAGGCATGT CCTGAACGGA 
GGTGTCAACG GAGTGGAGAT GAGCGCCTAT GCCAGCATAG ACTTTCTGTC TCACAACCTG 
AAAGAAGCAA GCGCTGTGAA AGGAAGTACA GGAAGATGGA AAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IRS2 ( XM_015277883.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133369.1 Gallus gallus uncharacterized LOC101749060 (LOC101749060), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa30700
Clone ID Related Accession (Same CDS sequence)	XM_015277883.2 , XM_015277883.1
Accession Version	XM_015277883.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	insulin receptor substrate 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763585.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCC CCGCCGCGCC GGGGCTGCCG CCCGGCCCCA ACCTGAACAA CAACAACAAC 
AACCGCGGCG TGAGGAAGTG CGGCTACCTG CGCAAGCAGA AGCACGGCCA CAAGCGCTTC 
TTCGTGCTGC GCGGCCCGGG CGGCGGCGGG GAGGAGGCGG GGGGCGCCCG GCTGGAGTAC 
TACGAGAGCG AGAAGAAATG GAGGAACAAG TCCGGGGCGC CCAAGCGGGT GATCGCGCTG 
GACTCGTGCC TCAACATCAA CAAGCGGGCG GACGCCAAGC ACAAGTACCT CATCGCCCTC 
TATACCAAGG ACGAGTACTT TGCCGTGGCG GCCGAGAACG AGCAGGAGCA GGAGGGCTGG 
TACCGCGCCC TCACCGACCT GCTGAGCGAG GGCAAGGCGG CCGCCGGCTC CCCGCACCGC 
CCCCTGCCCT CGCCCTTCGC CGCGCTGGCC GCCGGCGAGG AGCCCGACTA CGGGCTGATG 
ACGCCGGCCG CCGCCGCCTA CCGGGAGGTC TGGCAGGTGA CGCTGAAGCC CAAAGGCTTG 
GGGCAGAGCA AGAACCTCAC CGGCGTGCAC CGCCTCTGCC TCTCGGCCCG CACCATCGGC 
TTCGTGCGCC TCAACTGCGA GCTGCCCTCG GTCACGCTGC AGCTGATGAA CATCCGCCGC 
TGCGGCCACT CGGACAGCTT CTTCTTCATC GAGGTGGGCC GTTCGGCCGC CACCGGGCCC 
GGCGAGCTGT GGATGCAGGC GGACGACTCG GTGGTGGCCC AGAACATCCA CGAGACCATC 
CTGGAGGCTA TGAAGGCTCT AAAGGAGCTG TCCGAGTTCC GGCCCCGCAG CAAGAGCCAG 
TCGTCGTCCT CCTCTTCCTC CGGGGGGGCG GCCGGAGGCG GCGGCCCCTC GGCCACGCAC 
CCCATCACCG TGCCGGGCCG CCGGCACCAC CACCTGGTCA ACCTGCCGCC CAGCCAGACC 
GGCCTGCTCC GCCGATCCCG CACCGACAGC CTGGCCGCCG GCAGCAGGGC CGCGCCGTGC 
CGGGTGCGGA CGGCCAGCGA GGGCGACGGC TGCCGGGTGG GCTCGGCCGC CGGCAGCCCC 
ATGAGCCCCG GCCCCGTGCG GACCCCGCTC AGCCGCTCGC ACACGCTGAG CGGCGGCGGA 
GGGCGGCCGG CGGGCAAGCT GCTGCCCGTG CTGGCCGGGG GCGGGCTGCA AAGCAGCCGC 
TCCATGTCCA TGCCCGCCTC CCACTCGCCC CCCTCCGCCA CTAGCCCCGT CAGTCTCTCC 
TCCAGCAGCG GCCTGGGCTC CGAGCCGGCC CACCCGCATC ACCCGCAGCG CCCGTCCAGC 
GGCAGCGCCT CGGTGTCCGG CTCGCCCAGC GACGCGGGCT TCATGTCCTT CGACGAGTAC 
GGCTCCAGCC CGGGCGGCGA CCCGCGGCCC TTCTCCTCCT CCTCCACCGC CAGCAACCGC 
AGCAACACAC CCGAATCGGT GGCCGAGACC CCCCCGGTGC GGGACTCCGG CGGCGGCACC 
GATCTGTATG GCTACATGGC GATGGAGCGG CCCCCGAGCG GCCGTCTCTG CTACCGGCCT 
TGCCCCGAGG CTGACCGGGG CCACCGAAAG CGGACCTACT CGCTGACCAC CCCGTGCCGG 
CAGCGGCCCG CCCCGCCGCA GGTCTCCTCC GCCTCCCTGG ACGAGTACAC GCTAATGAGG 
GCCACCTTCG CCGGCAGCGC CGGCCGCCTC TTCCCCTCCT GCCCGGCAGG GGCTTCCCCT 
AAAGTCACCT ACACGCCGTA CCCCGAGGAC TACGGCGACA TCGAGATCGG CTCGCACCGC 
AGCTCCGGCA GCAGCAGCAC CAACCTGGGG CCGCCGGCAG GGGGGGGAGG CGGAGGCAGG 
GGCGGAGATG ACGATGGCTA CATGCCTATG ACCCCCGGCG TGGCCGCGGC CCTGGGTCGG 
GGGGGGCGGG GTGGCGATGA CTACATGCCC ATGAGCCCCA CCAGCGTGTC GGCCCCCAAA 
CAGATCCTGC AGCCCCGAGC GGGGGTGGGA GGCGGCTCCC CCGGCAACGG GAGCAGCTAT 
AAGACCAGCT CGCCGGGGGA AAGCTCCCCC GACGACAGCG GCTACATGAG GATGTGGTGC 
GGCTCTAAGC TGTCCGTGGA GAGTTCGGAC GGGAGGCTGA GCAACGGCGA CTACATCAAC 
ATGTCCCCTC GAGACCCCCA GCACGGGTCC CAGGCTCCCT CCCTCACCCC CCCGGACTTT 
TTCTTCGCCC CCGCAGGGCA CGGGGCTGGC GAGCCCCTCA AACCCGGCTG CTACTCTTAC 
AGCTCCCTGC CCCGCTCCTA CAAGAGCCAG GGCCCAGCTA AGGACAGCGA CCAGTACGTG 
TTCATGAACT CTCCGGGCAG GATGATCCCG GAGGAGGCGA TGTGCGGAGC GGTTCTGTCC 
CCAGCCGGCA CCTTCGCCCC CTCCAGCCAC ACGGTGCCTT CGCCCCTGCG GCACAGCCGG 
ACCGAGGGCT TCCTCAGCCA GCGGTGCCAG CGGGCCATCC GGCCCAGCCG CCTCTCTCTG 
GAGACCTTGC GGACGATGCT GCCAAGCATG AACGAGCACC CCTTGCCTCC CGAGCCCAAG 
AGCCCCGGGG AATACATCAA CATTGACTTC GGGGACACCG CTGTCTATTC TCCCCCCTCG 
CTGCCCACCG ACAGCCCAGC CTCCTCCCTG GGCTCAGGCA CAGGGCAGAG GCGCTCCCCT 
CTCTCCGACT ACATGAACAT CGACTTCAGC TCGCAGTCAC CCTCCCAGTC AGGCACAGTC 
TCGGTGGGCT CTTTGGAAGC GCTCTCGCCT GGCTCTTCCT CCAGCACCAG TCAGCCCGAT 
GGGCGCTACA TGAAGGCGGC TGGGGGGGTG GCCTGTCTGT CCAGCCCATC TGACAGCGGG 
GATTACACTG AGATGACCTT TGGCATGGCC ACTACCCCAC CCCAACCCAT CGTTCAGAAG 
CCAGAAAGTG CCCGGGTCAC CAGCCCCACG GCCGGGGTGA AGAGGCTCAC CCTCTCCGGG 
GTGGAGGCCT TCATTCTTTC CAGCCCACCC CCAGACCCCA ACCGCGGGGC CAAGGTCATT 
CGGGCTGACC CCCAGGGGCG CAGGAGGCAC AGCTCGGAAA CTTTCTCCTC CACCACCACT 
GTGACCCCCG TGTCCCCCTC CTTTGCACAC AACCCCAAAA GGCACAACTC AGCCTCGGTG 
GAGAACGTGT CCCTCAGGAA AAGCGAAGGC CTGGAGGAGG AGCAGGGCAG CAGCCCCATG 
TGCCGGGAGA CCTCGGCTGG CTTCCAGAAT GGCCTCAACT ACATCGCCGT CGACTTGGTG 
GACGGCAGCC TGGCAGGCTG TGACAAAGCC AGGTCAAAAG CCAGGCATGT CCTGAACGGA 
GGTGTCAACG GAGTGGAGAT GAGCGCCTAT GCCAGCATAG ACTTTCTGTC TCACAACCTG 
AAAGAAGCAA GCGCTGTGAA AGGAAGTACA GGAAGATGGA AAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133369.1
CDS	124..3591
Translation	MASPAAPGLPPGPNLNNNNNNRGVRKCGYLRKQKHGHKRFFVLRGPGGGGEEAGGARLEYYESEKKWRNKSGAPKRVIALDSCLNINKRADAKHKYLIALYTKDEYFAVAAENEQEQEGWYRALTDLLSEGKAAAGSPHRPLPSPFAALAAGEEPDYGLMTPAAAAYREVWQVTLKPKGLGQSKNLTGVHRLCLSARTIGFVRLNCELPSVTLQLMNIRRCGHSDSFFFIEVGRSAATGPGELWMQADDSVVAQNIHETILEAMKALKELSEFRPRSKSQSSSSSSSGGAAGGGGPSATHPITVPGRRHHHLVNLPPSQTGLLRRSRTDSLAAGSRAAPCRVRTASEGDGCRVGSAAGSPMSPGPVRTPLSRSHTLSGGGGRPAGKLLPVLAGGGLQSSRSMSMPASHSPPSATSPVSLSSSSGLGSEPAHPHHPQRPSSGSASVSGSPSDAGFMSFDEYGSSPGGDPRPFSSSSTASNRSNTPESVAETPPVRDSGGGTDLYGYMAMERPPSGRLCYRPCPEADRGHRKRTYSLTTPCRQRPAPPQVSSASLDEYTLMRATFAGSAGRLFPSCPAGASPKVTYTPYPEDYGDIEIGSHRSSGSSSTNLGPPAGGGGGGRGGDDDGYMPMTPGVAAALGRGGRGGDDYMPMSPTSVSAPKQILQPRAGVGGGSPGNGSSYKTSSPGESSPDDSGYMRMWCGSKLSVESSDGRLSNGDYINMSPRDPQHGSQAPSLTPPDFFFAPAGHGAGEPLKPGCYSYSSLPRSYKSQGPAKDSDQYVFMNSPGRMIPEEAMCGAVLSPAGTFAPSSHTVPSPLRHSRTEGFLSQRCQRAIRPSRLSLETLRTMLPSMNEHPLPPEPKSPGEYINIDFGDTAVYSPPSLPTDSPASSLGSGTGQRRSPLSDYMNIDFSSQSPSQSGTVSVGSLEALSPGSSSSTSQPDGRYMKAAGGVACLSSPSDSGDYTEMTFGMATTPPQPIVQKPESARVTSPTAGVKRLTLSGVEAFILSSPPPDPNRGAKVIRADPQGRRRHSSETFSSTTTVTPVSPSFAHNPKRHNSASVENVSLRKSEGLEEEQGSSPMCRETSAGFQNGLNYIAVDLVDGSLAGCDKARSKARHVLNGGVNGVEMSAYASIDFLSHNLKEASAVKGSTGRWKR

Target ORF information:

RefSeq Version	XM_015277883.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus uncharacterized LOC101749060 (LOC101749060), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277883.1

ORF Insert Sequence:

ATGGCGAGCC CCGCCGCGCC GGGGCTGCCG CCCGGCCCCA ACCTGAACAA CAACAACAAC 
AACCGCGGCG TGAGGAAGTG CGGCTACCTG CGCAAGCAGA AGCACGGCCA CAAGCGCTTC 
TTCGTGCTGC GCGGCCCGGG CGGCGGCGGG GAGGAGGCGG GGGGCGCCCG GCTGGAGTAC 
TACGAGAGCG AGAAGAAATG GAGGAACAAG TCCGGGGCGC CCAAGCGGGT GATCGCGCTG 
GACTCGTGCC TCAACATCAA CAAGCGGGCG GACGCCAAGC ACAAGTACCT CATCGCCCTC 
TATACCAAGG ACGAGTACTT TGCCGTGGCG GCCGAGAACG AGCAGGAGCA GGAGGGCTGG 
TACCGCGCCC TCACCGACCT GCTGAGCGAG GGCAAGGCGG CCGCCGGCTC CCCGCACCGC 
CCCCTGCCCT CGCCCTTCGC CGCGCTGGCC GCCGGCGAGG AGCCCGACTA CGGGCTGATG 
ACGCCGGCCG CCGCCGCCTA CCGGGAGGTC TGGCAGGTGA CGCTGAAGCC CAAAGGCTTG 
GGGCAGAGCA AGAACCTCAC CGGCGTGCAC CGCCTCTGCC TCTCGGCCCG CACCATCGGC 
TTCGTGCGCC TCAACTGCGA GCTGCCCTCG GTCACGCTGC AGCTGATGAA CATCCGCCGC 
TGCGGCCACT CGGACAGCTT CTTCTTCATC GAGGTGGGCC GTTCGGCCGC CACCGGGCCC 
GGCGAGCTGT GGATGCAGGC GGACGACTCG GTGGTGGCCC AGAACATCCA CGAGACCATC 
CTGGAGGCTA TGAAGGCTCT AAAGGAGCTG TCCGAGTTCC GGCCCCGCAG CAAGAGCCAG 
TCGTCGTCCT CCTCTTCCTC CGGGGGGGCG GCCGGAGGCG GCGGCCCCTC GGCCACGCAC 
CCCATCACCG TGCCGGGCCG CCGGCACCAC CACCTGGTCA ACCTGCCGCC CAGCCAGACC 
GGCCTGCTCC GCCGATCCCG CACCGACAGC CTGGCCGCCG GCAGCAGGGC CGCGCCGTGC 
CGGGTGCGGA CGGCCAGCGA GGGCGACGGC TGCCGGGTGG GCTCGGCCGC CGGCAGCCCC 
ATGAGCCCCG GCCCCGTGCG GACCCCGCTC AGCCGCTCGC ACACGCTGAG CGGCGGCGGA 
GGGCGGCCGG CGGGCAAGCT GCTGCCCGTG CTGGCCGGGG GCGGGCTGCA AAGCAGCCGC 
TCCATGTCCA TGCCCGCCTC CCACTCGCCC CCCTCCGCCA CTAGCCCCGT CAGTCTCTCC 
TCCAGCAGCG GCCTGGGCTC CGAGCCGGCC CACCCGCATC ACCCGCAGCG CCCGTCCAGC 
GGCAGCGCCT CGGTGTCCGG CTCGCCCAGC GACGCGGGCT TCATGTCCTT CGACGAGTAC 
GGCTCCAGCC CGGGCGGCGA CCCGCGGCCC TTCTCCTCCT CCTCCACCGC CAGCAACCGC 
AGCAACACAC CCGAATCGGT GGCCGAGACC CCCCCGGTGC GGGACTCCGG CGGCGGCACC 
GATCTGTATG GCTACATGGC GATGGAGCGG CCCCCGAGCG GCCGTCTCTG CTACCGGCCT 
TGCCCCGAGG CTGACCGGGG CCACCGAAAG CGGACCTACT CGCTGACCAC CCCGTGCCGG 
CAGCGGCCCG CCCCGCCGCA GGTCTCCTCC GCCTCCCTGG ACGAGTACAC GCTAATGAGG 
GCCACCTTCG CCGGCAGCGC CGGCCGCCTC TTCCCCTCCT GCCCGGCAGG GGCTTCCCCT 
AAAGTCACCT ACACGCCGTA CCCCGAGGAC TACGGCGACA TCGAGATCGG CTCGCACCGC 
AGCTCCGGCA GCAGCAGCAC CAACCTGGGG CCGCCGGCAG GGGGGGGAGG CGGAGGCAGG 
GGCGGAGATG ACGATGGCTA CATGCCTATG ACCCCCGGCG TGGCCGCGGC CCTGGGTCGG 
GGGGGGCGGG GTGGCGATGA CTACATGCCC ATGAGCCCCA CCAGCGTGTC GGCCCCCAAA 
CAGATCCTGC AGCCCCGAGC GGGGGTGGGA GGCGGCTCCC CCGGCAACGG GAGCAGCTAT 
AAGACCAGCT CGCCGGGGGA AAGCTCCCCC GACGACAGCG GCTACATGAG GATGTGGTGC 
GGCTCTAAGC TGTCCGTGGA GAGTTCGGAC GGGAGGCTGA GCAACGGCGA CTACATCAAC 
ATGTCCCCTC GAGACCCCCA GCACGGGTCC CAGGCTCCCT CCCTCACCCC CCCGGACTTT 
TTCTTCGCCC CCGCAGGGCA CGGGGCTGGC GAGCCCCTCA AACCCGGCTG CTACTCTTAC 
AGCTCCCTGC CCCGCTCCTA CAAGAGCCAG GGCCCAGCTA AGGACAGCGA CCAGTACGTG 
TTCATGAACT CTCCGGGCAG GATGATCCCG GAGGAGGCGA TGTGCGGAGC GGTTCTGTCC 
CCAGCCGGCA CCTTCGCCCC CTCCAGCCAC ACGGTGCCTT CGCCCCTGCG GCACAGCCGG 
ACCGAGGGCT TCCTCAGCCA GCGGTGCCAG CGGGCCATCC GGCCCAGCCG CCTCTCTCTG 
GAGACCTTGC GGACGATGCT GCCAAGCATG AACGAGCACC CCTTGCCTCC CGAGCCCAAG 
AGCCCCGGGG AATACATCAA CATTGACTTC GGGGACACCG CTGTCTATTC TCCCCCCTCG 
CTGCCCACCG ACAGCCCAGC CTCCTCCCTG GGCTCAGGCA CAGGGCAGAG GCGCTCCCCT 
CTCTCCGACT ACATGAACAT CGACTTCAGC TCGCAGTCAC CCTCCCAGTC AGGCACAGTC 
TCGGTGGGCT CTTTGGAAGC GCTCTCGCCT GGCTCTTCCT CCAGCACCAG TCAGCCCGAT 
GGGCGCTACA TGAAGGCGGC TGGGGGGGTG GCCTGTCTGT CCAGCCCATC TGACAGCGGG 
GATTACACTG AGATGACCTT TGGCATGGCC ACTACCCCAC CCCAACCCAT CGTTCAGAAG 
CCAGAAAGTG CCCGGGTCAC CAGCCCCACG GCCGGGGTGA AGAGGCTCAC CCTCTCCGGG 
GTGGAGGCCT TCATTCTTTC CAGCCCACCC CCAGACCCCA ACCGCGGGGC CAAGGTCATT 
CGGGCTGACC CCCAGGGGCG CAGGAGGCAC AGCTCGGAAA CTTTCTCCTC CACCACCACT 
GTGACCCCCG TGTCCCCCTC CTTTGCACAC AACCCCAAAA GGCACAACTC AGCCTCGGTG 
GAGAACGTGT CCCTCAGGAA AAGCGAAGGC CTGGAGGAGG AGCAGGGCAG CAGCCCCATG 
TGCCGGGAGA CCTCGGCTGG CTTCCAGAAT GGCCTCAACT ACATCGCCGT CGACTTGGTG 
GACGGCAGCC TGGCAGGCTG TGACAAAGCC AGGTCAAAAG CCAGGCATGT CCTGAACGGA 
GGTGTCAACG GAGTGGAGAT GAGCGCCTAT GCCAGCATAG ACTTTCTGTC TCACAACCTG 
AAAGAAGCAA GCGCTGTGAA AGGAAGTACA GGAAGATGGA AAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IRS2 ( XM_015277884.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133370.1 Gallus gallus uncharacterized LOC101749060 (LOC101749060), transcript variant X3, mRNA.

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CloneID	OGa30701
Clone ID Related Accession (Same CDS sequence)	XM_015277884.1 , XM_015277884.2
Accession Version	XM_015277884.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	insulin receptor substrate 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763585.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCC CCGCCGCGCC GGGGCTGCCG CCCGGCCCCA ACCTGAACAA CAACAACAAC 
AACCGCGGCG TGAGGAAGTG CGGCTACCTG CGCAAGCAGA AGCACGGCCA CAAGCGCTTC 
TTCGTGCTGC GCGGCCCGGG CGGCGGCGGG GAGGAGGCGG GGGGCGCCCG GCTGGAGTAC 
TACGAGAGCG AGAAGAAATG GAGGAACAAG TCCGGGGCGC CCAAGCGGGT GATCGCGCTG 
GACTCGTGCC TCAACATCAA CAAGCGGGCG GACGCCAAGC ACAAGTACCT CATCGCCCTC 
TATACCAAGG ACGAGTACTT TGCCGTGGCG GCCGAGAACG AGCAGGAGCA GGAGGGCTGG 
TACCGCGCCC TCACCGACCT GCTGAGCGAG GGCAAGGCGG CCGCCGGCTC CCCGCACCGC 
CCCCTGCCCT CGCCCTTCGC CGCGCTGGCC GCCGGCGAGG AGCCCGACTA CGGGCTGATG 
ACGCCGGCCG CCGCCGCCTA CCGGGAGGTC TGGCAGGTGA CGCTGAAGCC CAAAGGCTTG 
GGGCAGAGCA AGAACCTCAC CGGCGTGCAC CGCCTCTGCC TCTCGGCCCG CACCATCGGC 
TTCGTGCGCC TCAACTGCGA GCTGCCCTCG GTCACGCTGC AGCTGATGAA CATCCGCCGC 
TGCGGCCACT CGGACAGCTT CTTCTTCATC GAGGTGGGCC GTTCGGCCGC CACCGGGCCC 
GGCGAGCTGT GGATGCAGGC GGACGACTCG GTGGTGGCCC AGAACATCCA CGAGACCATC 
CTGGAGGCTA TGAAGGCTCT AAAGGAGCTG TCCGAGTTCC GGCCCCGCAG CAAGAGCCAG 
TCGTCGTCCT CCTCTTCCTC CGGGGGGGCG GCCGGAGGCG GCGGCCCCTC GGCCACGCAC 
CCCATCACCG TGCCGGGCCG CCGGCACCAC CACCTGGTCA ACCTGCCGCC CAGCCAGACC 
GGCCTGCTCC GCCGATCCCG CACCGACAGC CTGGCCGCCG GCAGCAGGGC CGCGCCGTGC 
CGGGTGCGGA CGGCCAGCGA GGGCGACGGC TGCCGGGTGG GCTCGGCCGC CGGCAGCCCC 
ATGAGCCCCG GCCCCGTGCG GACCCCGCTC AGCCGCTCGC ACACGCTGAG CGGCGGCGGA 
GGGCGGCCGG CGGGCAAGCT GCTGCCCGTG CTGGCCGGGG GCGGGCTGCA AAGCAGCCGC 
TCCATGTCCA TGCCCGCCTC CCACTCGCCC CCCTCCGCCA CTAGCCCCGT CAGTCTCTCC 
TCCAGCAGCG GCCTGGGCTC CGAGCCGGCC CACCCGCATC ACCCGCAGCG CCCGTCCAGC 
GGCAGCGCCT CGGTGTCCGG CTCGCCCAGC GACGCGGGCT TCATGTCCTT CGACGAGTAC 
GGCTCCAGCC CGGGCGGCGA CCCGCGGCCC TTCTCCTCCT CCTCCACCGC CAGCAACCGC 
AGCAACACAC CCGAATCGGT GGCCGAGACC CCCCCGGTGC GGGACTCCGG CGGCGGCACC 
GATCTGTATG GCTACATGGC GATGGAGCGG CCCCCGAGCG GCCGTCTCTG CTACCGGCCT 
TGCCCCGAGG CTGACCGGGG CCACCGAAAG CGGACCTACT CGCTGACCAC CCCGTGCCGG 
CAGCGGCCCG CCCCGCCGCA GGTCTCCTCC GCCTCCCTGG ACGAGTACAC GCTAATGAGG 
GCCACCTTCG CCGGCAGCGC CGGCCGCCTC TTCCCCTCCT GCCCGGCAGG GGCTTCCCCT 
AAAGTCACCT ACACGCCGTA CCCCGAGGAC TACGGCGACA TCGAGATCGG CTCGCACCGC 
AGCTCCGGCA GCAGCAGCAC CAACCTGGGG CCGCCGGCAG GGGGGGGAGG CGGAGGCAGG 
GGCGGAGATG ACGATGGCTA CATGCCTATG ACCCCCGGCG TGGCCGCGGC CCTGGGTCGG 
GGGGGGCGGG GTGGCGATGA CTACATGCCC ATGAGCCCCA CCAGCGTGTC GGCCCCCAAA 
CAGATCCTGC AGCCCCGAGC GGGGGTGGGA GGCGGCTCCC CCGGCAACGG GAGCAGCTAT 
AAGACCAGCT CGCCGGGGGA AAGCTCCCCC GACGACAGCG GCTACATGAG GATGTGGTGC 
GGCTCTAAGC TGTCCGTGGA GAGTTCGGAC GGGAGGCTGA GCAACGGCGA CTACATCAAC 
ATGTCCCCTC GAGACCCCCA GCACGGGTCC CAGGCTCCCT CCCTCACCCC CCCGGACTTT 
TTCTTCGCCC CCGCAGGGCA CGGGGCTGGC GAGCCCCTCA AACCCGGCTG CTACTCTTAC 
AGCTCCCTGC CCCGCTCCTA CAAGAGCCAG GGCCCAGCTA AGGACAGCGA CCAGTACGTG 
TTCATGAACT CTCCGGGCAG GATGATCCCG GAGGAGGCGA TGTGCGGAGC GGTTCTGTCC 
CCAGCCGGCA CCTTCGCCCC CTCCAGCCAC ACGGTGCCTT CGCCCCTGCG GCACAGCCGG 
ACCGAGGGCT TCCTCAGCCA GCGGTGCCAG CGGGCCATCC GGCCCAGCCG CCTCTCTCTG 
GAGACCTTGC GGACGATGCT GCCAAGCATG AACGAGCACC CCTTGCCTCC CGAGCCCAAG 
AGCCCCGGGG AATACATCAA CATTGACTTC GGGGACACCG CTGTCTATTC TCCCCCCTCG 
CTGCCCACCG ACAGCCCAGC CTCCTCCCTG GGCTCAGGCA CAGGGCAGAG GCGCTCCCCT 
CTCTCCGACT ACATGAACAT CGACTTCAGC TCGCAGTCAC CCTCCCAGTC AGGCACAGTC 
TCGGTGGGCT CTTTGGAAGC GCTCTCGCCT GGCTCTTCCT CCAGCACCAG TCAGCCCGAT 
GGGCGCTACA TGAAGGCGGC TGGGGGGGTG GCCTGTCTGT CCAGCCCATC TGACAGCGGG 
GATTACACTG AGATGACCTT TGGCATGGCC ACTACCCCAC CCCAACCCAT CGTTCAGAAG 
CCAGAAAGTG CCCGGGTCAC CAGCCCCACG GCCGGGGTGA AGAGGCTCAC CCTCTCCGGG 
GTGGAGGCCT TCATTCTTTC CAGCCCACCC CCAGACCCCA ACCGCGGGGC CAAGGTCATT 
CGGGCTGACC CCCAGGGGCG CAGGAGGCAC AGCTCGGAAA CTTTCTCCTC CACCACCACT 
GTGACCCCCG TGTCCCCCTC CTTTGCACAC AACCCCAAAA GGCACAACTC AGCCTCGGTG 
GAGAACGTGT CCCTCAGGAA AAGCGAAGGC CTGGAGGAGG AGCAGGGCAG CAGCCCCATG 
TGCCGGGAGA CCTCGGCTGG CTTCCAGAAT GGCCTCAACT ACATCGCCGT CGACTTGGTG 
GACGGCAGCC TGGCAGGCTG TGACAAAGCC AGGTCAAAAG CCAGGCATGT CCTGAACGGA 
GGTGTCAACG GAGTGGAGAT GAGCGCCTAT GCCAGCATAG ACTTTCTGTC TCACAACCTG 
AAAGAAGCAA GCGCTGTGAA AGCCAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133370.1
CDS	125..3574
Translation	MASPAAPGLPPGPNLNNNNNNRGVRKCGYLRKQKHGHKRFFVLRGPGGGGEEAGGARLEYYESEKKWRNKSGAPKRVIALDSCLNINKRADAKHKYLIALYTKDEYFAVAAENEQEQEGWYRALTDLLSEGKAAAGSPHRPLPSPFAALAAGEEPDYGLMTPAAAAYREVWQVTLKPKGLGQSKNLTGVHRLCLSARTIGFVRLNCELPSVTLQLMNIRRCGHSDSFFFIEVGRSAATGPGELWMQADDSVVAQNIHETILEAMKALKELSEFRPRSKSQSSSSSSSGGAAGGGGPSATHPITVPGRRHHHLVNLPPSQTGLLRRSRTDSLAAGSRAAPCRVRTASEGDGCRVGSAAGSPMSPGPVRTPLSRSHTLSGGGGRPAGKLLPVLAGGGLQSSRSMSMPASHSPPSATSPVSLSSSSGLGSEPAHPHHPQRPSSGSASVSGSPSDAGFMSFDEYGSSPGGDPRPFSSSSTASNRSNTPESVAETPPVRDSGGGTDLYGYMAMERPPSGRLCYRPCPEADRGHRKRTYSLTTPCRQRPAPPQVSSASLDEYTLMRATFAGSAGRLFPSCPAGASPKVTYTPYPEDYGDIEIGSHRSSGSSSTNLGPPAGGGGGGRGGDDDGYMPMTPGVAAALGRGGRGGDDYMPMSPTSVSAPKQILQPRAGVGGGSPGNGSSYKTSSPGESSPDDSGYMRMWCGSKLSVESSDGRLSNGDYINMSPRDPQHGSQAPSLTPPDFFFAPAGHGAGEPLKPGCYSYSSLPRSYKSQGPAKDSDQYVFMNSPGRMIPEEAMCGAVLSPAGTFAPSSHTVPSPLRHSRTEGFLSQRCQRAIRPSRLSLETLRTMLPSMNEHPLPPEPKSPGEYINIDFGDTAVYSPPSLPTDSPASSLGSGTGQRRSPLSDYMNIDFSSQSPSQSGTVSVGSLEALSPGSSSSTSQPDGRYMKAAGGVACLSSPSDSGDYTEMTFGMATTPPQPIVQKPESARVTSPTAGVKRLTLSGVEAFILSSPPPDPNRGAKVIRADPQGRRRHSSETFSSTTTVTPVSPSFAHNPKRHNSASVENVSLRKSEGLEEEQGSSPMCRETSAGFQNGLNYIAVDLVDGSLAGCDKARSKARHVLNGGVNGVEMSAYASIDFLSHNLKEASAVKAS

Target ORF information:

RefSeq Version	XM_015277884.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus uncharacterized LOC101749060 (LOC101749060), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277884.1

ORF Insert Sequence:

ATGGCGAGCC CCGCCGCGCC GGGGCTGCCG CCCGGCCCCA ACCTGAACAA CAACAACAAC 
AACCGCGGCG TGAGGAAGTG CGGCTACCTG CGCAAGCAGA AGCACGGCCA CAAGCGCTTC 
TTCGTGCTGC GCGGCCCGGG CGGCGGCGGG GAGGAGGCGG GGGGCGCCCG GCTGGAGTAC 
TACGAGAGCG AGAAGAAATG GAGGAACAAG TCCGGGGCGC CCAAGCGGGT GATCGCGCTG 
GACTCGTGCC TCAACATCAA CAAGCGGGCG GACGCCAAGC ACAAGTACCT CATCGCCCTC 
TATACCAAGG ACGAGTACTT TGCCGTGGCG GCCGAGAACG AGCAGGAGCA GGAGGGCTGG 
TACCGCGCCC TCACCGACCT GCTGAGCGAG GGCAAGGCGG CCGCCGGCTC CCCGCACCGC 
CCCCTGCCCT CGCCCTTCGC CGCGCTGGCC GCCGGCGAGG AGCCCGACTA CGGGCTGATG 
ACGCCGGCCG CCGCCGCCTA CCGGGAGGTC TGGCAGGTGA CGCTGAAGCC CAAAGGCTTG 
GGGCAGAGCA AGAACCTCAC CGGCGTGCAC CGCCTCTGCC TCTCGGCCCG CACCATCGGC 
TTCGTGCGCC TCAACTGCGA GCTGCCCTCG GTCACGCTGC AGCTGATGAA CATCCGCCGC 
TGCGGCCACT CGGACAGCTT CTTCTTCATC GAGGTGGGCC GTTCGGCCGC CACCGGGCCC 
GGCGAGCTGT GGATGCAGGC GGACGACTCG GTGGTGGCCC AGAACATCCA CGAGACCATC 
CTGGAGGCTA TGAAGGCTCT AAAGGAGCTG TCCGAGTTCC GGCCCCGCAG CAAGAGCCAG 
TCGTCGTCCT CCTCTTCCTC CGGGGGGGCG GCCGGAGGCG GCGGCCCCTC GGCCACGCAC 
CCCATCACCG TGCCGGGCCG CCGGCACCAC CACCTGGTCA ACCTGCCGCC CAGCCAGACC 
GGCCTGCTCC GCCGATCCCG CACCGACAGC CTGGCCGCCG GCAGCAGGGC CGCGCCGTGC 
CGGGTGCGGA CGGCCAGCGA GGGCGACGGC TGCCGGGTGG GCTCGGCCGC CGGCAGCCCC 
ATGAGCCCCG GCCCCGTGCG GACCCCGCTC AGCCGCTCGC ACACGCTGAG CGGCGGCGGA 
GGGCGGCCGG CGGGCAAGCT GCTGCCCGTG CTGGCCGGGG GCGGGCTGCA AAGCAGCCGC 
TCCATGTCCA TGCCCGCCTC CCACTCGCCC CCCTCCGCCA CTAGCCCCGT CAGTCTCTCC 
TCCAGCAGCG GCCTGGGCTC CGAGCCGGCC CACCCGCATC ACCCGCAGCG CCCGTCCAGC 
GGCAGCGCCT CGGTGTCCGG CTCGCCCAGC GACGCGGGCT TCATGTCCTT CGACGAGTAC 
GGCTCCAGCC CGGGCGGCGA CCCGCGGCCC TTCTCCTCCT CCTCCACCGC CAGCAACCGC 
AGCAACACAC CCGAATCGGT GGCCGAGACC CCCCCGGTGC GGGACTCCGG CGGCGGCACC 
GATCTGTATG GCTACATGGC GATGGAGCGG CCCCCGAGCG GCCGTCTCTG CTACCGGCCT 
TGCCCCGAGG CTGACCGGGG CCACCGAAAG CGGACCTACT CGCTGACCAC CCCGTGCCGG 
CAGCGGCCCG CCCCGCCGCA GGTCTCCTCC GCCTCCCTGG ACGAGTACAC GCTAATGAGG 
GCCACCTTCG CCGGCAGCGC CGGCCGCCTC TTCCCCTCCT GCCCGGCAGG GGCTTCCCCT 
AAAGTCACCT ACACGCCGTA CCCCGAGGAC TACGGCGACA TCGAGATCGG CTCGCACCGC 
AGCTCCGGCA GCAGCAGCAC CAACCTGGGG CCGCCGGCAG GGGGGGGAGG CGGAGGCAGG 
GGCGGAGATG ACGATGGCTA CATGCCTATG ACCCCCGGCG TGGCCGCGGC CCTGGGTCGG 
GGGGGGCGGG GTGGCGATGA CTACATGCCC ATGAGCCCCA CCAGCGTGTC GGCCCCCAAA 
CAGATCCTGC AGCCCCGAGC GGGGGTGGGA GGCGGCTCCC CCGGCAACGG GAGCAGCTAT 
AAGACCAGCT CGCCGGGGGA AAGCTCCCCC GACGACAGCG GCTACATGAG GATGTGGTGC 
GGCTCTAAGC TGTCCGTGGA GAGTTCGGAC GGGAGGCTGA GCAACGGCGA CTACATCAAC 
ATGTCCCCTC GAGACCCCCA GCACGGGTCC CAGGCTCCCT CCCTCACCCC CCCGGACTTT 
TTCTTCGCCC CCGCAGGGCA CGGGGCTGGC GAGCCCCTCA AACCCGGCTG CTACTCTTAC 
AGCTCCCTGC CCCGCTCCTA CAAGAGCCAG GGCCCAGCTA AGGACAGCGA CCAGTACGTG 
TTCATGAACT CTCCGGGCAG GATGATCCCG GAGGAGGCGA TGTGCGGAGC GGTTCTGTCC 
CCAGCCGGCA CCTTCGCCCC CTCCAGCCAC ACGGTGCCTT CGCCCCTGCG GCACAGCCGG 
ACCGAGGGCT TCCTCAGCCA GCGGTGCCAG CGGGCCATCC GGCCCAGCCG CCTCTCTCTG 
GAGACCTTGC GGACGATGCT GCCAAGCATG AACGAGCACC CCTTGCCTCC CGAGCCCAAG 
AGCCCCGGGG AATACATCAA CATTGACTTC GGGGACACCG CTGTCTATTC TCCCCCCTCG 
CTGCCCACCG ACAGCCCAGC CTCCTCCCTG GGCTCAGGCA CAGGGCAGAG GCGCTCCCCT 
CTCTCCGACT ACATGAACAT CGACTTCAGC TCGCAGTCAC CCTCCCAGTC AGGCACAGTC 
TCGGTGGGCT CTTTGGAAGC GCTCTCGCCT GGCTCTTCCT CCAGCACCAG TCAGCCCGAT 
GGGCGCTACA TGAAGGCGGC TGGGGGGGTG GCCTGTCTGT CCAGCCCATC TGACAGCGGG 
GATTACACTG AGATGACCTT TGGCATGGCC ACTACCCCAC CCCAACCCAT CGTTCAGAAG 
CCAGAAAGTG CCCGGGTCAC CAGCCCCACG GCCGGGGTGA AGAGGCTCAC CCTCTCCGGG 
GTGGAGGCCT TCATTCTTTC CAGCCCACCC CCAGACCCCA ACCGCGGGGC CAAGGTCATT 
CGGGCTGACC CCCAGGGGCG CAGGAGGCAC AGCTCGGAAA CTTTCTCCTC CACCACCACT 
GTGACCCCCG TGTCCCCCTC CTTTGCACAC AACCCCAAAA GGCACAACTC AGCCTCGGTG 
GAGAACGTGT CCCTCAGGAA AAGCGAAGGC CTGGAGGAGG AGCAGGGCAG CAGCCCCATG 
TGCCGGGAGA CCTCGGCTGG CTTCCAGAAT GGCCTCAACT ACATCGCCGT CGACTTGGTG 
GACGGCAGCC TGGCAGGCTG TGACAAAGCC AGGTCAAAAG CCAGGCATGT CCTGAACGGA 
GGTGTCAACG GAGTGGAGAT GAGCGCCTAT GCCAGCATAG ACTTTCTGTC TCACAACCTG 
AAAGAAGCAA GCGCTGTGAA AGCCAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IRS2 ( XM_015277884.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133370.1 Gallus gallus insulin receptor substrate 2 (IRS2), transcript variant X3, mRNA.

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CloneID	OGa30701
Clone ID Related Accession (Same CDS sequence)	XM_015277884.1 , XM_015277884.2
Accession Version	XM_015277884.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	insulin receptor substrate 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277884.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCC CCGCCGCGCC GGGGCTGCCG CCCGGCCCCA ACCTGAACAA CAACAACAAC 
AACCGCGGCG TGAGGAAGTG CGGCTACCTG CGCAAGCAGA AGCACGGCCA CAAGCGCTTC 
TTCGTGCTGC GCGGCCCGGG CGGCGGCGGG GAGGAGGCGG GGGGCGCCCG GCTGGAGTAC 
TACGAGAGCG AGAAGAAATG GAGGAACAAG TCCGGGGCGC CCAAGCGGGT GATCGCGCTG 
GACTCGTGCC TCAACATCAA CAAGCGGGCG GACGCCAAGC ACAAGTACCT CATCGCCCTC 
TATACCAAGG ACGAGTACTT TGCCGTGGCG GCCGAGAACG AGCAGGAGCA GGAGGGCTGG 
TACCGCGCCC TCACCGACCT GCTGAGCGAG GGCAAGGCGG CCGCCGGCTC CCCGCACCGC 
CCCCTGCCCT CGCCCTTCGC CGCGCTGGCC GCCGGCGAGG AGCCCGACTA CGGGCTGATG 
ACGCCGGCCG CCGCCGCCTA CCGGGAGGTC TGGCAGGTGA CGCTGAAGCC CAAAGGCTTG 
GGGCAGAGCA AGAACCTCAC CGGCGTGCAC CGCCTCTGCC TCTCGGCCCG CACCATCGGC 
TTCGTGCGCC TCAACTGCGA GCTGCCCTCG GTCACGCTGC AGCTGATGAA CATCCGCCGC 
TGCGGCCACT CGGACAGCTT CTTCTTCATC GAGGTGGGCC GTTCGGCCGC CACCGGGCCC 
GGCGAGCTGT GGATGCAGGC GGACGACTCG GTGGTGGCCC AGAACATCCA CGAGACCATC 
CTGGAGGCTA TGAAGGCTCT AAAGGAGCTG TCCGAGTTCC GGCCCCGCAG CAAGAGCCAG 
TCGTCGTCCT CCTCTTCCTC CGGGGGGGCG GCCGGAGGCG GCGGCCCCTC GGCCACGCAC 
CCCATCACCG TGCCGGGCCG CCGGCACCAC CACCTGGTCA ACCTGCCGCC CAGCCAGACC 
GGCCTGCTCC GCCGATCCCG CACCGACAGC CTGGCCGCCG GCAGCAGGGC CGCGCCGTGC 
CGGGTGCGGA CGGCCAGCGA GGGCGACGGC TGCCGGGTGG GCTCGGCCGC CGGCAGCCCC 
ATGAGCCCCG GCCCCGTGCG GACCCCGCTC AGCCGCTCGC ACACGCTGAG CGGCGGCGGA 
GGGCGGCCGG CGGGCAAGCT GCTGCCCGTG CTGGCCGGGG GCGGGCTGCA AAGCAGCCGC 
TCCATGTCCA TGCCCGCCTC CCACTCGCCC CCCTCCGCCA CTAGCCCCGT CAGTCTCTCC 
TCCAGCAGCG GCCTGGGCTC CGAGCCGGCC CACCCGCATC ACCCGCAGCG CCCGTCCAGC 
GGCAGCGCCT CGGTGTCCGG CTCGCCCAGC GACGCGGGCT TCATGTCCTT CGACGAGTAC 
GGCTCCAGCC CGGGCGGCGA CCCGCGGCCC TTCTCCTCCT CCTCCACCGC CAGCAACCGC 
AGCAACACAC CCGAATCGGT GGCCGAGACC CCCCCGGTGC GGGACTCCGG CGGCGGCACC 
GATCTGTATG GCTACATGGC GATGGAGCGG CCCCCGAGCG GCCGTCTCTG CTACCGGCCT 
TGCCCCGAGG CTGACCGGGG CCACCGAAAG CGGACCTACT CGCTGACCAC CCCGTGCCGG 
CAGCGGCCCG CCCCGCCGCA GGTCTCCTCC GCCTCCCTGG ACGAGTACAC GCTAATGAGG 
GCCACCTTCG CCGGCAGCGC CGGCCGCCTC TTCCCCTCCT GCCCGGCAGG GGCTTCCCCT 
AAAGTCACCT ACACGCCGTA CCCCGAGGAC TACGGCGACA TCGAGATCGG CTCGCACCGC 
AGCTCCGGCA GCAGCAGCAC CAACCTGGGG CCGCCGGCAG GGGGGGGAGG CGGAGGCAGG 
GGCGGAGATG ACGATGGCTA CATGCCTATG ACCCCCGGCG TGGCCGCGGC CCTGGGTCGG 
GGGGGGCGGG GTGGCGATGA CTACATGCCC ATGAGCCCCA CCAGCGTGTC GGCCCCCAAA 
CAGATCCTGC AGCCCCGAGC GGGGGTGGGA GGCGGCTCCC CCGGCAACGG GAGCAGCTAT 
AAGACCAGCT CGCCGGGGGA AAGCTCCCCC GACGACAGCG GCTACATGAG GATGTGGTGC 
GGCTCTAAGC TGTCCGTGGA GAGTTCGGAC GGGAGGCTGA GCAACGGCGA CTACATCAAC 
ATGTCCCCTC GAGACCCCCA GCACGGGTCC CAGGCTCCCT CCCTCACCCC CCCGGACTTT 
TTCTTCGCCC CCGCAGGGCA CGGGGCTGGC GAGCCCCTCA AACCCGGCTG CTACTCTTAC 
AGCTCCCTGC CCCGCTCCTA CAAGAGCCAG GGCCCAGCTA AGGACAGCGA CCAGTACGTG 
TTCATGAACT CTCCGGGCAG GATGATCCCG GAGGAGGCGA TGTGCGGAGC GGTTCTGTCC 
CCAGCCGGCA CCTTCGCCCC CTCCAGCCAC ACGGTGCCTT CGCCCCTGCG GCACAGCCGG 
ACCGAGGGCT TCCTCAGCCA GCGGTGCCAG CGGGCCATCC GGCCCAGCCG CCTCTCTCTG 
GAGACCTTGC GGACGATGCT GCCAAGCATG AACGAGCACC CCTTGCCTCC CGAGCCCAAG 
AGCCCCGGGG AATACATCAA CATTGACTTC GGGGACACCG CTGTCTATTC TCCCCCCTCG 
CTGCCCACCG ACAGCCCAGC CTCCTCCCTG GGCTCAGGCA CAGGGCAGAG GCGCTCCCCT 
CTCTCCGACT ACATGAACAT CGACTTCAGC TCGCAGTCAC CCTCCCAGTC AGGCACAGTC 
TCGGTGGGCT CTTTGGAAGC GCTCTCGCCT GGCTCTTCCT CCAGCACCAG TCAGCCCGAT 
GGGCGCTACA TGAAGGCGGC TGGGGGGGTG GCCTGTCTGT CCAGCCCATC TGACAGCGGG 
GATTACACTG AGATGACCTT TGGCATGGCC ACTACCCCAC CCCAACCCAT CGTTCAGAAG 
CCAGAAAGTG CCCGGGTCAC CAGCCCCACG GCCGGGGTGA AGAGGCTCAC CCTCTCCGGG 
GTGGAGGCCT TCATTCTTTC CAGCCCACCC CCAGACCCCA ACCGCGGGGC CAAGGTCATT 
CGGGCTGACC CCCAGGGGCG CAGGAGGCAC AGCTCGGAAA CTTTCTCCTC CACCACCACT 
GTGACCCCCG TGTCCCCCTC CTTTGCACAC AACCCCAAAA GGCACAACTC AGCCTCGGTG 
GAGAACGTGT CCCTCAGGAA AAGCGAAGGC CTGGAGGAGG AGCAGGGCAG CAGCCCCATG 
TGCCGGGAGA CCTCGGCTGG CTTCCAGAAT GGCCTCAACT ACATCGCCGT CGACTTGGTG 
GACGGCAGCC TGGCAGGCTG TGACAAAGCC AGGTCAAAAG CCAGGCATGT CCTGAACGGA 
GGTGTCAACG GAGTGGAGAT GAGCGCCTAT GCCAGCATAG ACTTTCTGTC TCACAACCTG 
AAAGAAGCAA GCGCTGTGAA AGCCAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133370.1
CDS	188..3637
Translation	MASPAAPGLPPGPNLNNNNNNRGVRKCGYLRKQKHGHKRFFVLRGPGGGGEEAGGARLEYYESEKKWRNKSGAPKRVIALDSCLNINKRADAKHKYLIALYTKDEYFAVAAENEQEQEGWYRALTDLLSEGKAAAGSPHRPLPSPFAALAAGEEPDYGLMTPAAAAYREVWQVTLKPKGLGQSKNLTGVHRLCLSARTIGFVRLNCELPSVTLQLMNIRRCGHSDSFFFIEVGRSAATGPGELWMQADDSVVAQNIHETILEAMKALKELSEFRPRSKSQSSSSSSSGGAAGGGGPSATHPITVPGRRHHHLVNLPPSQTGLLRRSRTDSLAAGSRAAPCRVRTASEGDGCRVGSAAGSPMSPGPVRTPLSRSHTLSGGGGRPAGKLLPVLAGGGLQSSRSMSMPASHSPPSATSPVSLSSSSGLGSEPAHPHHPQRPSSGSASVSGSPSDAGFMSFDEYGSSPGGDPRPFSSSSTASNRSNTPESVAETPPVRDSGGGTDLYGYMAMERPPSGRLCYRPCPEADRGHRKRTYSLTTPCRQRPAPPQVSSASLDEYTLMRATFAGSAGRLFPSCPAGASPKVTYTPYPEDYGDIEIGSHRSSGSSSTNLGPPAGGGGGGRGGDDDGYMPMTPGVAAALGRGGRGGDDYMPMSPTSVSAPKQILQPRAGVGGGSPGNGSSYKTSSPGESSPDDSGYMRMWCGSKLSVESSDGRLSNGDYINMSPRDPQHGSQAPSLTPPDFFFAPAGHGAGEPLKPGCYSYSSLPRSYKSQGPAKDSDQYVFMNSPGRMIPEEAMCGAVLSPAGTFAPSSHTVPSPLRHSRTEGFLSQRCQRAIRPSRLSLETLRTMLPSMNEHPLPPEPKSPGEYINIDFGDTAVYSPPSLPTDSPASSLGSGTGQRRSPLSDYMNIDFSSQSPSQSGTVSVGSLEALSPGSSSSTSQPDGRYMKAAGGVACLSSPSDSGDYTEMTFGMATTPPQPIVQKPESARVTSPTAGVKRLTLSGVEAFILSSPPPDPNRGAKVIRADPQGRRRHSSETFSSTTTVTPVSPSFAHNPKRHNSASVENVSLRKSEGLEEEQGSSPMCRETSAGFQNGLNYIAVDLVDGSLAGCDKARSKARHVLNGGVNGVEMSAYASIDFLSHNLKEASAVKAS

Target ORF information:

RefSeq Version	XM_015277884.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus insulin receptor substrate 2 (IRS2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277884.2

ORF Insert Sequence:

ATGGCGAGCC CCGCCGCGCC GGGGCTGCCG CCCGGCCCCA ACCTGAACAA CAACAACAAC 
AACCGCGGCG TGAGGAAGTG CGGCTACCTG CGCAAGCAGA AGCACGGCCA CAAGCGCTTC 
TTCGTGCTGC GCGGCCCGGG CGGCGGCGGG GAGGAGGCGG GGGGCGCCCG GCTGGAGTAC 
TACGAGAGCG AGAAGAAATG GAGGAACAAG TCCGGGGCGC CCAAGCGGGT GATCGCGCTG 
GACTCGTGCC TCAACATCAA CAAGCGGGCG GACGCCAAGC ACAAGTACCT CATCGCCCTC 
TATACCAAGG ACGAGTACTT TGCCGTGGCG GCCGAGAACG AGCAGGAGCA GGAGGGCTGG 
TACCGCGCCC TCACCGACCT GCTGAGCGAG GGCAAGGCGG CCGCCGGCTC CCCGCACCGC 
CCCCTGCCCT CGCCCTTCGC CGCGCTGGCC GCCGGCGAGG AGCCCGACTA CGGGCTGATG 
ACGCCGGCCG CCGCCGCCTA CCGGGAGGTC TGGCAGGTGA CGCTGAAGCC CAAAGGCTTG 
GGGCAGAGCA AGAACCTCAC CGGCGTGCAC CGCCTCTGCC TCTCGGCCCG CACCATCGGC 
TTCGTGCGCC TCAACTGCGA GCTGCCCTCG GTCACGCTGC AGCTGATGAA CATCCGCCGC 
TGCGGCCACT CGGACAGCTT CTTCTTCATC GAGGTGGGCC GTTCGGCCGC CACCGGGCCC 
GGCGAGCTGT GGATGCAGGC GGACGACTCG GTGGTGGCCC AGAACATCCA CGAGACCATC 
CTGGAGGCTA TGAAGGCTCT AAAGGAGCTG TCCGAGTTCC GGCCCCGCAG CAAGAGCCAG 
TCGTCGTCCT CCTCTTCCTC CGGGGGGGCG GCCGGAGGCG GCGGCCCCTC GGCCACGCAC 
CCCATCACCG TGCCGGGCCG CCGGCACCAC CACCTGGTCA ACCTGCCGCC CAGCCAGACC 
GGCCTGCTCC GCCGATCCCG CACCGACAGC CTGGCCGCCG GCAGCAGGGC CGCGCCGTGC 
CGGGTGCGGA CGGCCAGCGA GGGCGACGGC TGCCGGGTGG GCTCGGCCGC CGGCAGCCCC 
ATGAGCCCCG GCCCCGTGCG GACCCCGCTC AGCCGCTCGC ACACGCTGAG CGGCGGCGGA 
GGGCGGCCGG CGGGCAAGCT GCTGCCCGTG CTGGCCGGGG GCGGGCTGCA AAGCAGCCGC 
TCCATGTCCA TGCCCGCCTC CCACTCGCCC CCCTCCGCCA CTAGCCCCGT CAGTCTCTCC 
TCCAGCAGCG GCCTGGGCTC CGAGCCGGCC CACCCGCATC ACCCGCAGCG CCCGTCCAGC 
GGCAGCGCCT CGGTGTCCGG CTCGCCCAGC GACGCGGGCT TCATGTCCTT CGACGAGTAC 
GGCTCCAGCC CGGGCGGCGA CCCGCGGCCC TTCTCCTCCT CCTCCACCGC CAGCAACCGC 
AGCAACACAC CCGAATCGGT GGCCGAGACC CCCCCGGTGC GGGACTCCGG CGGCGGCACC 
GATCTGTATG GCTACATGGC GATGGAGCGG CCCCCGAGCG GCCGTCTCTG CTACCGGCCT 
TGCCCCGAGG CTGACCGGGG CCACCGAAAG CGGACCTACT CGCTGACCAC CCCGTGCCGG 
CAGCGGCCCG CCCCGCCGCA GGTCTCCTCC GCCTCCCTGG ACGAGTACAC GCTAATGAGG 
GCCACCTTCG CCGGCAGCGC CGGCCGCCTC TTCCCCTCCT GCCCGGCAGG GGCTTCCCCT 
AAAGTCACCT ACACGCCGTA CCCCGAGGAC TACGGCGACA TCGAGATCGG CTCGCACCGC 
AGCTCCGGCA GCAGCAGCAC CAACCTGGGG CCGCCGGCAG GGGGGGGAGG CGGAGGCAGG 
GGCGGAGATG ACGATGGCTA CATGCCTATG ACCCCCGGCG TGGCCGCGGC CCTGGGTCGG 
GGGGGGCGGG GTGGCGATGA CTACATGCCC ATGAGCCCCA CCAGCGTGTC GGCCCCCAAA 
CAGATCCTGC AGCCCCGAGC GGGGGTGGGA GGCGGCTCCC CCGGCAACGG GAGCAGCTAT 
AAGACCAGCT CGCCGGGGGA AAGCTCCCCC GACGACAGCG GCTACATGAG GATGTGGTGC 
GGCTCTAAGC TGTCCGTGGA GAGTTCGGAC GGGAGGCTGA GCAACGGCGA CTACATCAAC 
ATGTCCCCTC GAGACCCCCA GCACGGGTCC CAGGCTCCCT CCCTCACCCC CCCGGACTTT 
TTCTTCGCCC CCGCAGGGCA CGGGGCTGGC GAGCCCCTCA AACCCGGCTG CTACTCTTAC 
AGCTCCCTGC CCCGCTCCTA CAAGAGCCAG GGCCCAGCTA AGGACAGCGA CCAGTACGTG 
TTCATGAACT CTCCGGGCAG GATGATCCCG GAGGAGGCGA TGTGCGGAGC GGTTCTGTCC 
CCAGCCGGCA CCTTCGCCCC CTCCAGCCAC ACGGTGCCTT CGCCCCTGCG GCACAGCCGG 
ACCGAGGGCT TCCTCAGCCA GCGGTGCCAG CGGGCCATCC GGCCCAGCCG CCTCTCTCTG 
GAGACCTTGC GGACGATGCT GCCAAGCATG AACGAGCACC CCTTGCCTCC CGAGCCCAAG 
AGCCCCGGGG AATACATCAA CATTGACTTC GGGGACACCG CTGTCTATTC TCCCCCCTCG 
CTGCCCACCG ACAGCCCAGC CTCCTCCCTG GGCTCAGGCA CAGGGCAGAG GCGCTCCCCT 
CTCTCCGACT ACATGAACAT CGACTTCAGC TCGCAGTCAC CCTCCCAGTC AGGCACAGTC 
TCGGTGGGCT CTTTGGAAGC GCTCTCGCCT GGCTCTTCCT CCAGCACCAG TCAGCCCGAT 
GGGCGCTACA TGAAGGCGGC TGGGGGGGTG GCCTGTCTGT CCAGCCCATC TGACAGCGGG 
GATTACACTG AGATGACCTT TGGCATGGCC ACTACCCCAC CCCAACCCAT CGTTCAGAAG 
CCAGAAAGTG CCCGGGTCAC CAGCCCCACG GCCGGGGTGA AGAGGCTCAC CCTCTCCGGG 
GTGGAGGCCT TCATTCTTTC CAGCCCACCC CCAGACCCCA ACCGCGGGGC CAAGGTCATT 
CGGGCTGACC CCCAGGGGCG CAGGAGGCAC AGCTCGGAAA CTTTCTCCTC CACCACCACT 
GTGACCCCCG TGTCCCCCTC CTTTGCACAC AACCCCAAAA GGCACAACTC AGCCTCGGTG 
GAGAACGTGT CCCTCAGGAA AAGCGAAGGC CTGGAGGAGG AGCAGGGCAG CAGCCCCATG 
TGCCGGGAGA CCTCGGCTGG CTTCCAGAAT GGCCTCAACT ACATCGCCGT CGACTTGGTG 
GACGGCAGCC TGGCAGGCTG TGACAAAGCC AGGTCAAAAG CCAGGCATGT CCTGAACGGA 
GGTGTCAACG GAGTGGAGAT GAGCGCCTAT GCCAGCATAG ACTTTCTGTC TCACAACCTG 
AAAGAAGCAA GCGCTGTGAA AGCCAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IRS2 ( XM_015277886.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133372.1 Gallus gallus uncharacterized LOC101749060 (LOC101749060), transcript variant X4, mRNA.

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CloneID	OGa30702
Clone ID Related Accession (Same CDS sequence)	XM_015277886.1 , XM_015277886.2
Accession Version	XM_015277886.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	insulin receptor substrate 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763585.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCC CCGCCGCGCC GGGGCTGCCG CCCGGCCCCA ACCTGAACAA CAACAACAAC 
AACCGCGGCG TGAGGAAGTG CGGCTACCTG CGCAAGCAGA AGCACGGCCA CAAGCGCTTC 
TTCGTGCTGC GCGGCCCGGG CGGCGGCGGG GAGGAGGCGG GGGGCGCCCG GCTGGAGTAC 
TACGAGAGCG AGAAGAAATG GAGGAACAAG TCCGGGGCGC CCAAGCGGGT GATCGCGCTG 
GACTCGTGCC TCAACATCAA CAAGCGGGCG GACGCCAAGC ACAAGTACCT CATCGCCCTC 
TATACCAAGG ACGAGTACTT TGCCGTGGCG GCCGAGAACG AGCAGGAGCA GGAGGGCTGG 
TACCGCGCCC TCACCGACCT GCTGAGCGAG GGCAAGGCGG CCGCCGGCTC CCCGCACCGC 
CCCCTGCCCT CGCCCTTCGC CGCGCTGGCC GCCGGCGAGG AGCCCGACTA CGGGCTGATG 
ACGCCGGCCG CCGCCGCCTA CCGGGAGGTC TGGCAGGTGA CGCTGAAGCC CAAAGGCTTG 
GGGCAGAGCA AGAACCTCAC CGGCGTGCAC CGCCTCTGCC TCTCGGCCCG CACCATCGGC 
TTCGTGCGCC TCAACTGCGA GCTGCCCTCG GTCACGCTGC AGCTGATGAA CATCCGCCGC 
TGCGGCCACT CGGACAGCTT CTTCTTCATC GAGGTGGGCC GTTCGGCCGC CACCGGGCCC 
GGCGAGCTGT GGATGCAGGC GGACGACTCG GTGGTGGCCC AGAACATCCA CGAGACCATC 
CTGGAGGCTA TGAAGGCTCT AAAGGAGCTG TCCGAGTTCC GGCCCCGCAG CAAGAGCCAG 
TCGTCGTCCT CCTCTTCCTC CGGGGGGGCG GCCGGAGGCG GCGGCCCCTC GGCCACGCAC 
CCCATCACCG TGCCGGGCCG CCGGCACCAC CACCTGGTCA ACCTGCCGCC CAGCCAGACC 
GGCCTGCTCC GCCGATCCCG CACCGACAGC CTGGCCGCCG GCAGCAGGGC CGCGCCGTGC 
CGGGTGCGGA CGGCCAGCGA GGGCGACGGC TGCCGGGTGG GCTCGGCCGC CGGCAGCCCC 
ATGAGCCCCG GCCCCGTGCG GACCCCGCTC AGCCGCTCGC ACACGCTGAG CGGCGGCGGA 
GGGCGGCCGG CGGGCAAGCT GCTGCCCGTG CTGGCCGGGG GCGGGCTGCA AAGCAGCCGC 
TCCATGTCCA TGCCCGCCTC CCACTCGCCC CCCTCCGCCA CTAGCCCCGT CAGTCTCTCC 
TCCAGCAGCG GCCTGGGCTC CGAGCCGGCC CACCCGCATC ACCCGCAGCG CCCGTCCAGC 
GGCAGCGCCT CGGTGTCCGG CTCGCCCAGC GACGCGGGCT TCATGTCCTT CGACGAGTAC 
GGCTCCAGCC CGGGCGGCGA CCCGCGGCCC TTCTCCTCCT CCTCCACCGC CAGCAACCGC 
AGCAACACAC CCGAATCGGT GGCCGAGACC CCCCCGGTGC GGGACTCCGG CGGCGGCACC 
GATCTGTATG GCTACATGGC GATGGAGCGG CCCCCGAGCG GCCGTCTCTG CTACCGGCCT 
TGCCCCGAGG CTGACCGGGG CCACCGAAAG CGGACCTACT CGCTGACCAC CCCGTGCCGG 
CAGCGGCCCG CCCCGCCGCA GGTCTCCTCC GCCTCCCTGG ACGAGTACAC GCTAATGAGG 
GCCACCTTCG CCGGCAGCGC CGGCCGCCTC TTCCCCTCCT GCCCGGCAGG GGCTTCCCCT 
AAAGTCACCT ACACGCCGTA CCCCGAGGAC TACGGCGACA TCGAGATCGG CTCGCACCGC 
AGCTCCGGCA GCAGCAGCAC CAACCTGGGG CCGCCGGCAG GGGGGGGAGG CGGAGGCAGG 
GGCGGAGATG ACGATGGCTA CATGCCTATG ACCCCCGGCG TGGCCGCGGC CCTGGGTCGG 
GGGGGGCGGG GTGGCGATGA CTACATGCCC ATGAGCCCCA CCAGCGTGTC GGCCCCCAAA 
CAGATCCTGC AGCCCCGAGC GGGGGTGGGA GGCGGCTCCC CCGGCAACGG GAGCAGCTAT 
AAGACCAGCT CGCCGGGGGA AAGCTCCCCC GACGACAGCG GCTACATGAG GATGTGGTGC 
GGCTCTAAGC TGTCCGTGGA GAGTTCGGAC GGGAGGCTGA GCAACGGCGA CTACATCAAC 
ATGTCCCCTC GAGACCCCCA GCACGGGTCC CAGGCTCCCT CCCTCACCCC CCCGGACTTT 
TTCTTCGCCC CCGCAGGGCA CGGGGCTGGC GAGCCCCTCA AACCCGGCTG CTACTCTTAC 
AGCTCCCTGC CCCGCTCCTA CAAGAGCCAG GGCCCAGCTA AGGACAGCGA CCAGTACGTG 
TTCATGAACT CTCCGGGCAG GATGATCCCG GAGGAGGCGA TGTGCGGAGC GGTTCTGTCC 
CCAGCCGGCA CCTTCGCCCC CTCCAGCCAC ACGGTGCCTT CGCCCCTGCG GCACAGCCGG 
ACCGAGGGCT TCCTCAGCCA GCGGTGCCAG CGGGCCATCC GGCCCAGCCG CCTCTCTCTG 
GAGACCTTGC GGACGATGCT GCCAAGCATG AACGAGCACC CCTTGCCTCC CGAGCCCAAG 
AGCCCCGGGG AATACATCAA CATTGACTTC GGGGACACCG CTGTCTATTC TCCCCCCTCG 
CTGCCCACCG ACAGCCCAGC CTCCTCCCTG GGCTCAGGCA CAGGGCAGAG GCGCTCCCCT 
CTCTCCGACT ACATGAACAT CGACTTCAGC TCGCAGTCAC CCTCCCAGTC AGGCACAGTC 
TCGGTGGGCT CTTTGGAAGC GCTCTCGCCT GGCTCTTCCT CCAGCACCAG TCAGCCCGAT 
GGGCGCTACA TGAAGGCGGC TGGGGGGGTG GCCTGTCTGT CCAGCCCATC TGACAGCGGG 
GATTACACTG AGATGACCTT TGGCATGGCC ACTACCCCAC CCCAACCCAT CGTTCAGAAG 
CCAGAAAGTG CCCGGGTCAC CAGCCCCACG GCCGGGGTGA AGAGGCTCAC CCTCTCCGGG 
GTGGAGGCCT TCATTCTTTC CAGCCCACCC CCAGACCCCA ACCGCGGGGC CAAGGTCATT 
CGGGCTGACC CCCAGGGGCG CAGGAGGCAC AGCTCGGAAA CTTTCTCCTC CACCACCACT 
GTGACCCCCG TGTCCCCCTC CTTTGCACAC AACCCCAAAA GGCACAACTC AGCCTCGGTG 
GAGAACGTGT CCCTCAGGAA AAGCGAAGGC CTGGAGGAGG AGCAGGGCAG CAGCCCCATG 
TGCCGGGAGA CCTCGGCTGG CTTCCAGAAT GGCCTCAACT ACATCGCCGT CGACTTGGTG 
GACGGCAGCC TGGCAGGCTG TGACAAAGCC AGGTCAAAAG CCAGGCATGT CCTGAACGGA 
GGTGTCAACG GAGTGGAGAT GAGCGCCTAT GCCAGCATAG ACTTTCTGTC TCACAACCTG 
AAAGAAGCAA GCGCTGTGAA AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133372.1
CDS	124..3570
Translation	MASPAAPGLPPGPNLNNNNNNRGVRKCGYLRKQKHGHKRFFVLRGPGGGGEEAGGARLEYYESEKKWRNKSGAPKRVIALDSCLNINKRADAKHKYLIALYTKDEYFAVAAENEQEQEGWYRALTDLLSEGKAAAGSPHRPLPSPFAALAAGEEPDYGLMTPAAAAYREVWQVTLKPKGLGQSKNLTGVHRLCLSARTIGFVRLNCELPSVTLQLMNIRRCGHSDSFFFIEVGRSAATGPGELWMQADDSVVAQNIHETILEAMKALKELSEFRPRSKSQSSSSSSSGGAAGGGGPSATHPITVPGRRHHHLVNLPPSQTGLLRRSRTDSLAAGSRAAPCRVRTASEGDGCRVGSAAGSPMSPGPVRTPLSRSHTLSGGGGRPAGKLLPVLAGGGLQSSRSMSMPASHSPPSATSPVSLSSSSGLGSEPAHPHHPQRPSSGSASVSGSPSDAGFMSFDEYGSSPGGDPRPFSSSSTASNRSNTPESVAETPPVRDSGGGTDLYGYMAMERPPSGRLCYRPCPEADRGHRKRTYSLTTPCRQRPAPPQVSSASLDEYTLMRATFAGSAGRLFPSCPAGASPKVTYTPYPEDYGDIEIGSHRSSGSSSTNLGPPAGGGGGGRGGDDDGYMPMTPGVAAALGRGGRGGDDYMPMSPTSVSAPKQILQPRAGVGGGSPGNGSSYKTSSPGESSPDDSGYMRMWCGSKLSVESSDGRLSNGDYINMSPRDPQHGSQAPSLTPPDFFFAPAGHGAGEPLKPGCYSYSSLPRSYKSQGPAKDSDQYVFMNSPGRMIPEEAMCGAVLSPAGTFAPSSHTVPSPLRHSRTEGFLSQRCQRAIRPSRLSLETLRTMLPSMNEHPLPPEPKSPGEYINIDFGDTAVYSPPSLPTDSPASSLGSGTGQRRSPLSDYMNIDFSSQSPSQSGTVSVGSLEALSPGSSSSTSQPDGRYMKAAGGVACLSSPSDSGDYTEMTFGMATTPPQPIVQKPESARVTSPTAGVKRLTLSGVEAFILSSPPPDPNRGAKVIRADPQGRRRHSSETFSSTTTVTPVSPSFAHNPKRHNSASVENVSLRKSEGLEEEQGSSPMCRETSAGFQNGLNYIAVDLVDGSLAGCDKARSKARHVLNGGVNGVEMSAYASIDFLSHNLKEASAVKE

Target ORF information:

RefSeq Version	XM_015277886.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus uncharacterized LOC101749060 (LOC101749060), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277886.1

ORF Insert Sequence:

ATGGCGAGCC CCGCCGCGCC GGGGCTGCCG CCCGGCCCCA ACCTGAACAA CAACAACAAC 
AACCGCGGCG TGAGGAAGTG CGGCTACCTG CGCAAGCAGA AGCACGGCCA CAAGCGCTTC 
TTCGTGCTGC GCGGCCCGGG CGGCGGCGGG GAGGAGGCGG GGGGCGCCCG GCTGGAGTAC 
TACGAGAGCG AGAAGAAATG GAGGAACAAG TCCGGGGCGC CCAAGCGGGT GATCGCGCTG 
GACTCGTGCC TCAACATCAA CAAGCGGGCG GACGCCAAGC ACAAGTACCT CATCGCCCTC 
TATACCAAGG ACGAGTACTT TGCCGTGGCG GCCGAGAACG AGCAGGAGCA GGAGGGCTGG 
TACCGCGCCC TCACCGACCT GCTGAGCGAG GGCAAGGCGG CCGCCGGCTC CCCGCACCGC 
CCCCTGCCCT CGCCCTTCGC CGCGCTGGCC GCCGGCGAGG AGCCCGACTA CGGGCTGATG 
ACGCCGGCCG CCGCCGCCTA CCGGGAGGTC TGGCAGGTGA CGCTGAAGCC CAAAGGCTTG 
GGGCAGAGCA AGAACCTCAC CGGCGTGCAC CGCCTCTGCC TCTCGGCCCG CACCATCGGC 
TTCGTGCGCC TCAACTGCGA GCTGCCCTCG GTCACGCTGC AGCTGATGAA CATCCGCCGC 
TGCGGCCACT CGGACAGCTT CTTCTTCATC GAGGTGGGCC GTTCGGCCGC CACCGGGCCC 
GGCGAGCTGT GGATGCAGGC GGACGACTCG GTGGTGGCCC AGAACATCCA CGAGACCATC 
CTGGAGGCTA TGAAGGCTCT AAAGGAGCTG TCCGAGTTCC GGCCCCGCAG CAAGAGCCAG 
TCGTCGTCCT CCTCTTCCTC CGGGGGGGCG GCCGGAGGCG GCGGCCCCTC GGCCACGCAC 
CCCATCACCG TGCCGGGCCG CCGGCACCAC CACCTGGTCA ACCTGCCGCC CAGCCAGACC 
GGCCTGCTCC GCCGATCCCG CACCGACAGC CTGGCCGCCG GCAGCAGGGC CGCGCCGTGC 
CGGGTGCGGA CGGCCAGCGA GGGCGACGGC TGCCGGGTGG GCTCGGCCGC CGGCAGCCCC 
ATGAGCCCCG GCCCCGTGCG GACCCCGCTC AGCCGCTCGC ACACGCTGAG CGGCGGCGGA 
GGGCGGCCGG CGGGCAAGCT GCTGCCCGTG CTGGCCGGGG GCGGGCTGCA AAGCAGCCGC 
TCCATGTCCA TGCCCGCCTC CCACTCGCCC CCCTCCGCCA CTAGCCCCGT CAGTCTCTCC 
TCCAGCAGCG GCCTGGGCTC CGAGCCGGCC CACCCGCATC ACCCGCAGCG CCCGTCCAGC 
GGCAGCGCCT CGGTGTCCGG CTCGCCCAGC GACGCGGGCT TCATGTCCTT CGACGAGTAC 
GGCTCCAGCC CGGGCGGCGA CCCGCGGCCC TTCTCCTCCT CCTCCACCGC CAGCAACCGC 
AGCAACACAC CCGAATCGGT GGCCGAGACC CCCCCGGTGC GGGACTCCGG CGGCGGCACC 
GATCTGTATG GCTACATGGC GATGGAGCGG CCCCCGAGCG GCCGTCTCTG CTACCGGCCT 
TGCCCCGAGG CTGACCGGGG CCACCGAAAG CGGACCTACT CGCTGACCAC CCCGTGCCGG 
CAGCGGCCCG CCCCGCCGCA GGTCTCCTCC GCCTCCCTGG ACGAGTACAC GCTAATGAGG 
GCCACCTTCG CCGGCAGCGC CGGCCGCCTC TTCCCCTCCT GCCCGGCAGG GGCTTCCCCT 
AAAGTCACCT ACACGCCGTA CCCCGAGGAC TACGGCGACA TCGAGATCGG CTCGCACCGC 
AGCTCCGGCA GCAGCAGCAC CAACCTGGGG CCGCCGGCAG GGGGGGGAGG CGGAGGCAGG 
GGCGGAGATG ACGATGGCTA CATGCCTATG ACCCCCGGCG TGGCCGCGGC CCTGGGTCGG 
GGGGGGCGGG GTGGCGATGA CTACATGCCC ATGAGCCCCA CCAGCGTGTC GGCCCCCAAA 
CAGATCCTGC AGCCCCGAGC GGGGGTGGGA GGCGGCTCCC CCGGCAACGG GAGCAGCTAT 
AAGACCAGCT CGCCGGGGGA AAGCTCCCCC GACGACAGCG GCTACATGAG GATGTGGTGC 
GGCTCTAAGC TGTCCGTGGA GAGTTCGGAC GGGAGGCTGA GCAACGGCGA CTACATCAAC 
ATGTCCCCTC GAGACCCCCA GCACGGGTCC CAGGCTCCCT CCCTCACCCC CCCGGACTTT 
TTCTTCGCCC CCGCAGGGCA CGGGGCTGGC GAGCCCCTCA AACCCGGCTG CTACTCTTAC 
AGCTCCCTGC CCCGCTCCTA CAAGAGCCAG GGCCCAGCTA AGGACAGCGA CCAGTACGTG 
TTCATGAACT CTCCGGGCAG GATGATCCCG GAGGAGGCGA TGTGCGGAGC GGTTCTGTCC 
CCAGCCGGCA CCTTCGCCCC CTCCAGCCAC ACGGTGCCTT CGCCCCTGCG GCACAGCCGG 
ACCGAGGGCT TCCTCAGCCA GCGGTGCCAG CGGGCCATCC GGCCCAGCCG CCTCTCTCTG 
GAGACCTTGC GGACGATGCT GCCAAGCATG AACGAGCACC CCTTGCCTCC CGAGCCCAAG 
AGCCCCGGGG AATACATCAA CATTGACTTC GGGGACACCG CTGTCTATTC TCCCCCCTCG 
CTGCCCACCG ACAGCCCAGC CTCCTCCCTG GGCTCAGGCA CAGGGCAGAG GCGCTCCCCT 
CTCTCCGACT ACATGAACAT CGACTTCAGC TCGCAGTCAC CCTCCCAGTC AGGCACAGTC 
TCGGTGGGCT CTTTGGAAGC GCTCTCGCCT GGCTCTTCCT CCAGCACCAG TCAGCCCGAT 
GGGCGCTACA TGAAGGCGGC TGGGGGGGTG GCCTGTCTGT CCAGCCCATC TGACAGCGGG 
GATTACACTG AGATGACCTT TGGCATGGCC ACTACCCCAC CCCAACCCAT CGTTCAGAAG 
CCAGAAAGTG CCCGGGTCAC CAGCCCCACG GCCGGGGTGA AGAGGCTCAC CCTCTCCGGG 
GTGGAGGCCT TCATTCTTTC CAGCCCACCC CCAGACCCCA ACCGCGGGGC CAAGGTCATT 
CGGGCTGACC CCCAGGGGCG CAGGAGGCAC AGCTCGGAAA CTTTCTCCTC CACCACCACT 
GTGACCCCCG TGTCCCCCTC CTTTGCACAC AACCCCAAAA GGCACAACTC AGCCTCGGTG 
GAGAACGTGT CCCTCAGGAA AAGCGAAGGC CTGGAGGAGG AGCAGGGCAG CAGCCCCATG 
TGCCGGGAGA CCTCGGCTGG CTTCCAGAAT GGCCTCAACT ACATCGCCGT CGACTTGGTG 
GACGGCAGCC TGGCAGGCTG TGACAAAGCC AGGTCAAAAG CCAGGCATGT CCTGAACGGA 
GGTGTCAACG GAGTGGAGAT GAGCGCCTAT GCCAGCATAG ACTTTCTGTC TCACAACCTG 
AAAGAAGCAA GCGCTGTGAA AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IRS2 ( XM_015277886.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133372.1 Gallus gallus insulin receptor substrate 2 (IRS2), transcript variant X4, mRNA.

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CloneID	OGa30702
Clone ID Related Accession (Same CDS sequence)	XM_015277886.1 , XM_015277886.2
Accession Version	XM_015277886.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	insulin receptor substrate 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277886.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCC CCGCCGCGCC GGGGCTGCCG CCCGGCCCCA ACCTGAACAA CAACAACAAC 
AACCGCGGCG TGAGGAAGTG CGGCTACCTG CGCAAGCAGA AGCACGGCCA CAAGCGCTTC 
TTCGTGCTGC GCGGCCCGGG CGGCGGCGGG GAGGAGGCGG GGGGCGCCCG GCTGGAGTAC 
TACGAGAGCG AGAAGAAATG GAGGAACAAG TCCGGGGCGC CCAAGCGGGT GATCGCGCTG 
GACTCGTGCC TCAACATCAA CAAGCGGGCG GACGCCAAGC ACAAGTACCT CATCGCCCTC 
TATACCAAGG ACGAGTACTT TGCCGTGGCG GCCGAGAACG AGCAGGAGCA GGAGGGCTGG 
TACCGCGCCC TCACCGACCT GCTGAGCGAG GGCAAGGCGG CCGCCGGCTC CCCGCACCGC 
CCCCTGCCCT CGCCCTTCGC CGCGCTGGCC GCCGGCGAGG AGCCCGACTA CGGGCTGATG 
ACGCCGGCCG CCGCCGCCTA CCGGGAGGTC TGGCAGGTGA CGCTGAAGCC CAAAGGCTTG 
GGGCAGAGCA AGAACCTCAC CGGCGTGCAC CGCCTCTGCC TCTCGGCCCG CACCATCGGC 
TTCGTGCGCC TCAACTGCGA GCTGCCCTCG GTCACGCTGC AGCTGATGAA CATCCGCCGC 
TGCGGCCACT CGGACAGCTT CTTCTTCATC GAGGTGGGCC GTTCGGCCGC CACCGGGCCC 
GGCGAGCTGT GGATGCAGGC GGACGACTCG GTGGTGGCCC AGAACATCCA CGAGACCATC 
CTGGAGGCTA TGAAGGCTCT AAAGGAGCTG TCCGAGTTCC GGCCCCGCAG CAAGAGCCAG 
TCGTCGTCCT CCTCTTCCTC CGGGGGGGCG GCCGGAGGCG GCGGCCCCTC GGCCACGCAC 
CCCATCACCG TGCCGGGCCG CCGGCACCAC CACCTGGTCA ACCTGCCGCC CAGCCAGACC 
GGCCTGCTCC GCCGATCCCG CACCGACAGC CTGGCCGCCG GCAGCAGGGC CGCGCCGTGC 
CGGGTGCGGA CGGCCAGCGA GGGCGACGGC TGCCGGGTGG GCTCGGCCGC CGGCAGCCCC 
ATGAGCCCCG GCCCCGTGCG GACCCCGCTC AGCCGCTCGC ACACGCTGAG CGGCGGCGGA 
GGGCGGCCGG CGGGCAAGCT GCTGCCCGTG CTGGCCGGGG GCGGGCTGCA AAGCAGCCGC 
TCCATGTCCA TGCCCGCCTC CCACTCGCCC CCCTCCGCCA CTAGCCCCGT CAGTCTCTCC 
TCCAGCAGCG GCCTGGGCTC CGAGCCGGCC CACCCGCATC ACCCGCAGCG CCCGTCCAGC 
GGCAGCGCCT CGGTGTCCGG CTCGCCCAGC GACGCGGGCT TCATGTCCTT CGACGAGTAC 
GGCTCCAGCC CGGGCGGCGA CCCGCGGCCC TTCTCCTCCT CCTCCACCGC CAGCAACCGC 
AGCAACACAC CCGAATCGGT GGCCGAGACC CCCCCGGTGC GGGACTCCGG CGGCGGCACC 
GATCTGTATG GCTACATGGC GATGGAGCGG CCCCCGAGCG GCCGTCTCTG CTACCGGCCT 
TGCCCCGAGG CTGACCGGGG CCACCGAAAG CGGACCTACT CGCTGACCAC CCCGTGCCGG 
CAGCGGCCCG CCCCGCCGCA GGTCTCCTCC GCCTCCCTGG ACGAGTACAC GCTAATGAGG 
GCCACCTTCG CCGGCAGCGC CGGCCGCCTC TTCCCCTCCT GCCCGGCAGG GGCTTCCCCT 
AAAGTCACCT ACACGCCGTA CCCCGAGGAC TACGGCGACA TCGAGATCGG CTCGCACCGC 
AGCTCCGGCA GCAGCAGCAC CAACCTGGGG CCGCCGGCAG GGGGGGGAGG CGGAGGCAGG 
GGCGGAGATG ACGATGGCTA CATGCCTATG ACCCCCGGCG TGGCCGCGGC CCTGGGTCGG 
GGGGGGCGGG GTGGCGATGA CTACATGCCC ATGAGCCCCA CCAGCGTGTC GGCCCCCAAA 
CAGATCCTGC AGCCCCGAGC GGGGGTGGGA GGCGGCTCCC CCGGCAACGG GAGCAGCTAT 
AAGACCAGCT CGCCGGGGGA AAGCTCCCCC GACGACAGCG GCTACATGAG GATGTGGTGC 
GGCTCTAAGC TGTCCGTGGA GAGTTCGGAC GGGAGGCTGA GCAACGGCGA CTACATCAAC 
ATGTCCCCTC GAGACCCCCA GCACGGGTCC CAGGCTCCCT CCCTCACCCC CCCGGACTTT 
TTCTTCGCCC CCGCAGGGCA CGGGGCTGGC GAGCCCCTCA AACCCGGCTG CTACTCTTAC 
AGCTCCCTGC CCCGCTCCTA CAAGAGCCAG GGCCCAGCTA AGGACAGCGA CCAGTACGTG 
TTCATGAACT CTCCGGGCAG GATGATCCCG GAGGAGGCGA TGTGCGGAGC GGTTCTGTCC 
CCAGCCGGCA CCTTCGCCCC CTCCAGCCAC ACGGTGCCTT CGCCCCTGCG GCACAGCCGG 
ACCGAGGGCT TCCTCAGCCA GCGGTGCCAG CGGGCCATCC GGCCCAGCCG CCTCTCTCTG 
GAGACCTTGC GGACGATGCT GCCAAGCATG AACGAGCACC CCTTGCCTCC CGAGCCCAAG 
AGCCCCGGGG AATACATCAA CATTGACTTC GGGGACACCG CTGTCTATTC TCCCCCCTCG 
CTGCCCACCG ACAGCCCAGC CTCCTCCCTG GGCTCAGGCA CAGGGCAGAG GCGCTCCCCT 
CTCTCCGACT ACATGAACAT CGACTTCAGC TCGCAGTCAC CCTCCCAGTC AGGCACAGTC 
TCGGTGGGCT CTTTGGAAGC GCTCTCGCCT GGCTCTTCCT CCAGCACCAG TCAGCCCGAT 
GGGCGCTACA TGAAGGCGGC TGGGGGGGTG GCCTGTCTGT CCAGCCCATC TGACAGCGGG 
GATTACACTG AGATGACCTT TGGCATGGCC ACTACCCCAC CCCAACCCAT CGTTCAGAAG 
CCAGAAAGTG CCCGGGTCAC CAGCCCCACG GCCGGGGTGA AGAGGCTCAC CCTCTCCGGG 
GTGGAGGCCT TCATTCTTTC CAGCCCACCC CCAGACCCCA ACCGCGGGGC CAAGGTCATT 
CGGGCTGACC CCCAGGGGCG CAGGAGGCAC AGCTCGGAAA CTTTCTCCTC CACCACCACT 
GTGACCCCCG TGTCCCCCTC CTTTGCACAC AACCCCAAAA GGCACAACTC AGCCTCGGTG 
GAGAACGTGT CCCTCAGGAA AAGCGAAGGC CTGGAGGAGG AGCAGGGCAG CAGCCCCATG 
TGCCGGGAGA CCTCGGCTGG CTTCCAGAAT GGCCTCAACT ACATCGCCGT CGACTTGGTG 
GACGGCAGCC TGGCAGGCTG TGACAAAGCC AGGTCAAAAG CCAGGCATGT CCTGAACGGA 
GGTGTCAACG GAGTGGAGAT GAGCGCCTAT GCCAGCATAG ACTTTCTGTC TCACAACCTG 
AAAGAAGCAA GCGCTGTGAA AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133372.1
CDS	187..3633
Translation	MASPAAPGLPPGPNLNNNNNNRGVRKCGYLRKQKHGHKRFFVLRGPGGGGEEAGGARLEYYESEKKWRNKSGAPKRVIALDSCLNINKRADAKHKYLIALYTKDEYFAVAAENEQEQEGWYRALTDLLSEGKAAAGSPHRPLPSPFAALAAGEEPDYGLMTPAAAAYREVWQVTLKPKGLGQSKNLTGVHRLCLSARTIGFVRLNCELPSVTLQLMNIRRCGHSDSFFFIEVGRSAATGPGELWMQADDSVVAQNIHETILEAMKALKELSEFRPRSKSQSSSSSSSGGAAGGGGPSATHPITVPGRRHHHLVNLPPSQTGLLRRSRTDSLAAGSRAAPCRVRTASEGDGCRVGSAAGSPMSPGPVRTPLSRSHTLSGGGGRPAGKLLPVLAGGGLQSSRSMSMPASHSPPSATSPVSLSSSSGLGSEPAHPHHPQRPSSGSASVSGSPSDAGFMSFDEYGSSPGGDPRPFSSSSTASNRSNTPESVAETPPVRDSGGGTDLYGYMAMERPPSGRLCYRPCPEADRGHRKRTYSLTTPCRQRPAPPQVSSASLDEYTLMRATFAGSAGRLFPSCPAGASPKVTYTPYPEDYGDIEIGSHRSSGSSSTNLGPPAGGGGGGRGGDDDGYMPMTPGVAAALGRGGRGGDDYMPMSPTSVSAPKQILQPRAGVGGGSPGNGSSYKTSSPGESSPDDSGYMRMWCGSKLSVESSDGRLSNGDYINMSPRDPQHGSQAPSLTPPDFFFAPAGHGAGEPLKPGCYSYSSLPRSYKSQGPAKDSDQYVFMNSPGRMIPEEAMCGAVLSPAGTFAPSSHTVPSPLRHSRTEGFLSQRCQRAIRPSRLSLETLRTMLPSMNEHPLPPEPKSPGEYINIDFGDTAVYSPPSLPTDSPASSLGSGTGQRRSPLSDYMNIDFSSQSPSQSGTVSVGSLEALSPGSSSSTSQPDGRYMKAAGGVACLSSPSDSGDYTEMTFGMATTPPQPIVQKPESARVTSPTAGVKRLTLSGVEAFILSSPPPDPNRGAKVIRADPQGRRRHSSETFSSTTTVTPVSPSFAHNPKRHNSASVENVSLRKSEGLEEEQGSSPMCRETSAGFQNGLNYIAVDLVDGSLAGCDKARSKARHVLNGGVNGVEMSAYASIDFLSHNLKEASAVKE

Target ORF information:

RefSeq Version	XM_015277886.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus insulin receptor substrate 2 (IRS2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277886.2

ORF Insert Sequence:

ATGGCGAGCC CCGCCGCGCC GGGGCTGCCG CCCGGCCCCA ACCTGAACAA CAACAACAAC 
AACCGCGGCG TGAGGAAGTG CGGCTACCTG CGCAAGCAGA AGCACGGCCA CAAGCGCTTC 
TTCGTGCTGC GCGGCCCGGG CGGCGGCGGG GAGGAGGCGG GGGGCGCCCG GCTGGAGTAC 
TACGAGAGCG AGAAGAAATG GAGGAACAAG TCCGGGGCGC CCAAGCGGGT GATCGCGCTG 
GACTCGTGCC TCAACATCAA CAAGCGGGCG GACGCCAAGC ACAAGTACCT CATCGCCCTC 
TATACCAAGG ACGAGTACTT TGCCGTGGCG GCCGAGAACG AGCAGGAGCA GGAGGGCTGG 
TACCGCGCCC TCACCGACCT GCTGAGCGAG GGCAAGGCGG CCGCCGGCTC CCCGCACCGC 
CCCCTGCCCT CGCCCTTCGC CGCGCTGGCC GCCGGCGAGG AGCCCGACTA CGGGCTGATG 
ACGCCGGCCG CCGCCGCCTA CCGGGAGGTC TGGCAGGTGA CGCTGAAGCC CAAAGGCTTG 
GGGCAGAGCA AGAACCTCAC CGGCGTGCAC CGCCTCTGCC TCTCGGCCCG CACCATCGGC 
TTCGTGCGCC TCAACTGCGA GCTGCCCTCG GTCACGCTGC AGCTGATGAA CATCCGCCGC 
TGCGGCCACT CGGACAGCTT CTTCTTCATC GAGGTGGGCC GTTCGGCCGC CACCGGGCCC 
GGCGAGCTGT GGATGCAGGC GGACGACTCG GTGGTGGCCC AGAACATCCA CGAGACCATC 
CTGGAGGCTA TGAAGGCTCT AAAGGAGCTG TCCGAGTTCC GGCCCCGCAG CAAGAGCCAG 
TCGTCGTCCT CCTCTTCCTC CGGGGGGGCG GCCGGAGGCG GCGGCCCCTC GGCCACGCAC 
CCCATCACCG TGCCGGGCCG CCGGCACCAC CACCTGGTCA ACCTGCCGCC CAGCCAGACC 
GGCCTGCTCC GCCGATCCCG CACCGACAGC CTGGCCGCCG GCAGCAGGGC CGCGCCGTGC 
CGGGTGCGGA CGGCCAGCGA GGGCGACGGC TGCCGGGTGG GCTCGGCCGC CGGCAGCCCC 
ATGAGCCCCG GCCCCGTGCG GACCCCGCTC AGCCGCTCGC ACACGCTGAG CGGCGGCGGA 
GGGCGGCCGG CGGGCAAGCT GCTGCCCGTG CTGGCCGGGG GCGGGCTGCA AAGCAGCCGC 
TCCATGTCCA TGCCCGCCTC CCACTCGCCC CCCTCCGCCA CTAGCCCCGT CAGTCTCTCC 
TCCAGCAGCG GCCTGGGCTC CGAGCCGGCC CACCCGCATC ACCCGCAGCG CCCGTCCAGC 
GGCAGCGCCT CGGTGTCCGG CTCGCCCAGC GACGCGGGCT TCATGTCCTT CGACGAGTAC 
GGCTCCAGCC CGGGCGGCGA CCCGCGGCCC TTCTCCTCCT CCTCCACCGC CAGCAACCGC 
AGCAACACAC CCGAATCGGT GGCCGAGACC CCCCCGGTGC GGGACTCCGG CGGCGGCACC 
GATCTGTATG GCTACATGGC GATGGAGCGG CCCCCGAGCG GCCGTCTCTG CTACCGGCCT 
TGCCCCGAGG CTGACCGGGG CCACCGAAAG CGGACCTACT CGCTGACCAC CCCGTGCCGG 
CAGCGGCCCG CCCCGCCGCA GGTCTCCTCC GCCTCCCTGG ACGAGTACAC GCTAATGAGG 
GCCACCTTCG CCGGCAGCGC CGGCCGCCTC TTCCCCTCCT GCCCGGCAGG GGCTTCCCCT 
AAAGTCACCT ACACGCCGTA CCCCGAGGAC TACGGCGACA TCGAGATCGG CTCGCACCGC 
AGCTCCGGCA GCAGCAGCAC CAACCTGGGG CCGCCGGCAG GGGGGGGAGG CGGAGGCAGG 
GGCGGAGATG ACGATGGCTA CATGCCTATG ACCCCCGGCG TGGCCGCGGC CCTGGGTCGG 
GGGGGGCGGG GTGGCGATGA CTACATGCCC ATGAGCCCCA CCAGCGTGTC GGCCCCCAAA 
CAGATCCTGC AGCCCCGAGC GGGGGTGGGA GGCGGCTCCC CCGGCAACGG GAGCAGCTAT 
AAGACCAGCT CGCCGGGGGA AAGCTCCCCC GACGACAGCG GCTACATGAG GATGTGGTGC 
GGCTCTAAGC TGTCCGTGGA GAGTTCGGAC GGGAGGCTGA GCAACGGCGA CTACATCAAC 
ATGTCCCCTC GAGACCCCCA GCACGGGTCC CAGGCTCCCT CCCTCACCCC CCCGGACTTT 
TTCTTCGCCC CCGCAGGGCA CGGGGCTGGC GAGCCCCTCA AACCCGGCTG CTACTCTTAC 
AGCTCCCTGC CCCGCTCCTA CAAGAGCCAG GGCCCAGCTA AGGACAGCGA CCAGTACGTG 
TTCATGAACT CTCCGGGCAG GATGATCCCG GAGGAGGCGA TGTGCGGAGC GGTTCTGTCC 
CCAGCCGGCA CCTTCGCCCC CTCCAGCCAC ACGGTGCCTT CGCCCCTGCG GCACAGCCGG 
ACCGAGGGCT TCCTCAGCCA GCGGTGCCAG CGGGCCATCC GGCCCAGCCG CCTCTCTCTG 
GAGACCTTGC GGACGATGCT GCCAAGCATG AACGAGCACC CCTTGCCTCC CGAGCCCAAG 
AGCCCCGGGG AATACATCAA CATTGACTTC GGGGACACCG CTGTCTATTC TCCCCCCTCG 
CTGCCCACCG ACAGCCCAGC CTCCTCCCTG GGCTCAGGCA CAGGGCAGAG GCGCTCCCCT 
CTCTCCGACT ACATGAACAT CGACTTCAGC TCGCAGTCAC CCTCCCAGTC AGGCACAGTC 
TCGGTGGGCT CTTTGGAAGC GCTCTCGCCT GGCTCTTCCT CCAGCACCAG TCAGCCCGAT 
GGGCGCTACA TGAAGGCGGC TGGGGGGGTG GCCTGTCTGT CCAGCCCATC TGACAGCGGG 
GATTACACTG AGATGACCTT TGGCATGGCC ACTACCCCAC CCCAACCCAT CGTTCAGAAG 
CCAGAAAGTG CCCGGGTCAC CAGCCCCACG GCCGGGGTGA AGAGGCTCAC CCTCTCCGGG 
GTGGAGGCCT TCATTCTTTC CAGCCCACCC CCAGACCCCA ACCGCGGGGC CAAGGTCATT 
CGGGCTGACC CCCAGGGGCG CAGGAGGCAC AGCTCGGAAA CTTTCTCCTC CACCACCACT 
GTGACCCCCG TGTCCCCCTC CTTTGCACAC AACCCCAAAA GGCACAACTC AGCCTCGGTG 
GAGAACGTGT CCCTCAGGAA AAGCGAAGGC CTGGAGGAGG AGCAGGGCAG CAGCCCCATG 
TGCCGGGAGA CCTCGGCTGG CTTCCAGAAT GGCCTCAACT ACATCGCCGT CGACTTGGTG 
GACGGCAGCC TGGCAGGCTG TGACAAAGCC AGGTCAAAAG CCAGGCATGT CCTGAACGGA 
GGTGTCAACG GAGTGGAGAT GAGCGCCTAT GCCAGCATAG ACTTTCTGTC TCACAACCTG 
AAAGAAGCAA GCGCTGTGAA AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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IRS2 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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