NLRC5 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	NLRC5
Entrez Gene ID	100857413
Full Name	NLR family CARD domain containing 5
General protein information	Preferred Names NLR family CARD domain containing 5 Names protein NLRC5
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004944054.2	XP_004944111.1	protein NLRC5 isoform X1
XM_015292328.1	XP_015147814.1	protein NLRC5 isoform X1
NM_001318435.1	NP_001305364.1	protein NLRC5
XM_015292327.1	XP_015147813.1	protein NLRC5 isoform X1
XM_004944056.2	XP_004944113.1	protein NLRC5 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	NLRC5	NP_115582.4
Gallus gallus (chicken)	NLRC5	XP_004944113.1
Bos taurus (cattle)	NLRC5	XP_002694836.1
Danio rerio (zebrafish)	si:ch211-122c9.5	XP_003200494.2
Xenopus tropicalis (tropical clawed frog)	LOC101730474	XP_004914291.1
Pan troglodytes (chimpanzee)	NLRC5	XP_003952898.1
	NLRC5	XP_001095341.2
Mus musculus (house mouse)	Nlrc5	NP_001028379.2
Canis lupus familiaris (dog)	NLRC5	XP_544394.4

Gene Ontology
Bibliography

Related articles in PubMed

Expression patterns of NLRC5 and key genes in the STAT1 pathway following infection with Salmonella pullorum.
Qiu L, Ma T, Chang G, Liu X, Guo X, Xu L, Zhang Y, Zhao W, Xu Q, Chen G
Gene59723-29(2017 Jan)

Characterization of the NLRC5 promoter in chicken: SNPs, regulatory elements and CpG islands.
Guo XM, Liu XP, Chang GB, Xu L, Bi YL, Wang HZ, Zhang Y, Zhu PF, Wu Y, Chen GH
Animal genetics47(5)579-87(2016 Oct)

A mutation in the NLRC5 promoter limits NF-?B signaling after Salmonella Enteritidis infection in the spleen of young chickens.
Chang G, Liu X, Ma T, Xu L, Wang H, Li Z, Guo X, Xu Q, Chen G
Gene568(2)117-23(2015 Sep)

NLRC5 knockdown in chicken macrophages alters response to LPS and poly (I:C) stimulation.
Lian L, Ciraci C, Chang G, Hu J, Lamont SJ
BMC veterinary research823(2012 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Expression pattern of NLRC5 following infection with Salmonella pullorum.STAT1 plays crucial role in NLRC5 promoter activity.
Title: Expression patterns of NLRC5 and key genes in the STAT1 pathway following infection with Salmonella pullorum.

study of two potentially regulatory regions of the NLRC5 promoter-namely -4372 to -3756 and -2925 to -2265-that were essential in controlling NLRC5 gene expression; the cis-element STAT1, especially one in the second region, has a critical effect on NLRC5 transcription
Title: Characterization of the NLRC5 promoter in chicken: SNPs, regulatory elements and CpG islands.

NLRC5 wild genotype birds presented with better resistance to Salmonella Enteritidis through the actions of the NLRC5 and subsequent inhibition of the NF-kappaB pathway in chickens.
Title: A mutation in the NLRC5 promoter limits NF-?B signaling after Salmonella Enteritidis infection in the spleen of young chickens.

The knockdown of NLRC5 expression negatively mediates gene expression of interferon alpha and interferon beta in the chicken HD11 cell line.
Title: NLRC5 knockdown in chicken macrophages alters response to LPS and poly (I:C) stimulation.

The following NLRC5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NLRC5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa62918	NM_001318435.1 Latest version!	Gallus gallus NLR family CARD domain containing 5 (NLRC5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa13511	XM_015292327.1 Latest version!	Gallus gallus NLR family, CARD domain containing 5 (NLRC5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa13511	XM_004944054.2 Latest version!	Gallus gallus NLR family, CARD domain containing 5 (NLRC5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa13511	XM_015292328.1 Latest version!	Gallus gallus NLR family, CARD domain containing 5 (NLRC5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa13512	XM_004944056.2 Latest version!	Gallus gallus NLR family, CARD domain containing 5 (NLRC5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NLRC5 ( NM_001318435.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001305364.1 Gallus gallus NLR family CARD domain containing 5 (NLRC5), mRNA.

Price & Availability↑

CloneID	OGa62918
Clone ID Related Accession (Same CDS sequence)	NM_001318435.1
Accession Version	NM_001318435.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-20
Organism	Gallus gallus(chicken)
Product	protein NLRC5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from JQ044414.1. On Jan 6, 2016 this sequence version replaced XM_004944055.2. ##Evidence-Data-START## Transcript exon combination :: JQ044414.1 [ECO:0000332] ##Evidence-Data-END##

ATGCAGCCCA CACTCCAGAA CGATGACTTC AACCTGGAAG GAGCTGTAGC AAGCATCCGC 
CCCCAGCTAG TGGAGTTCCT CAGCCATCGT CTCGACTGGC TGCTCTCTGC TTCCCAGCAC 
TTCCTCCCTA CGGCTGTCCT CAGCGGCTTG GCAGGCGTCA CTGACCACAG AGAGAAAGTC 
TCTGTGCTCC TCGACCTGCT GGAGAAGGCT GGCCATGCCA CCTGGAAACA GTTTGCACAG 
TGCCTCTGCA TGGAGTGTGA CCTGCCCCTG GAAATGGAGA TACTGCTCAT GAGTTCTGCA 
GGAGAAGGTA ACTTGACTCA GAAGCAAGAA GCTCAGACAG ACGCAAGTGA CCAGTCCTCT 
GTGCCAAGAG GGTTGACGCG CCGCCGCCGT AGCAGCAGCC CCATCAGTGA TAAGGGTGCT 
AAACAGAAAC GCCTGGACTC AGCTGAAAAG TATCAACGTC TTCTCGTTGA CTCCATCTGC 
AAGAGATATG GAAGCAGGAG AGCAGCAGGA GCAGCGGCAC AAGAGCCAAC GCAGCCTCTG 
GCTTTCAGCC AAGCCTTTGT CAACCTCGTT CTTCGGCAGA GCAAGGCCTC CCGACTGAAA 
GAGAGAACAG ACAAACCCCG GGAGGACGTG CCCAGTGCCC CCGAGCCAGA AGAATGTGTG 
GATACAGCCG TGAGAGTGTC AGATCTCTTT GGCAGCGTGG TCCGATCGGG TACAACGAAG 
GTGATCTTTT TGTTTGGTAA ACCAGGTACA GGCAAGACCA TGCTGATGCA CAGGATTTGT 
CAGAAGTGGG CGGAAGGTGT CTTACATCAG TTTCTCTTCA CTTTCCTGTT TGAGTTCCGG 
CAGTTGAATT TACTAAAAAG AAAACTGACT CTGAAGGAGC TTTTGTTTGA CCTGTTTCTT 
CAGCCAGAAG ACAGCCCCGA TGCAGTGTTC CAGCACCTCC TGGAAAACGC CCAACGTACA 
CTGATCATCT TTGATGGGCT GGATGAGTTT GTGGGTAACA TGGACGTGTC ATCAGCTTCT 
AAAGGAAGCC TGAACCGTAG CTCCAGTATG TCCATATCTG AACTCTTTGC AGAGCTTTGT 
CATGGGAACC TCCTACCTGG CTGCACGGTG TTGGTCACCA GCCGCCCTAA GAGAGTCCCT 
GACTTCTTGA TAAACACAGT AGATTTGCTG GCAGAAGTTT GGGGGTTTGA TCACGAGAAG 
GTTGAGGAGT ATGTCAGCCA CTATTTTCGT CATCATTCAT TCAAAGAACA AGCCATCGCT 
CACCTGAAGA ACAACACCAA GCTCCTCAGC ATGTGCCAGA TCCCTGCCCT GTGTTACGTT 
GTCTGCATCT GTTTGGAGTA TTTGCTCCTT AAGCATCAGA CGAGCGTGGA GCTTCCCCAG 
ACCATGACAC AGTTTTATAT CAAAATGCTT CTCATCTTCA TAAACAAACA GCAGGGGGAG 
CATGCAGGTG ATGAGGAGGC TCAGCTGAAC AGTAACAAGA AGGCCATTTT GGGTCTGTGT 
GATCTTGCAC TGAAAGGCCT GGAAGCTAAG AAGCTCGTGT TTTACGTTGG AGATATTCCA 
GAGCACGTGA AAGAGTTTGC TTCCTTGCAC GGTCTGCTGA CTGTCTTTGA GGTGAAGACA 
AACAGCGCTC ACCCCGAGAC TGGCTATGCC TTTGTGCACT TGAGCCTGCA GGAGTTTTTT 
GCAGCCCTGT GTCTCATGAT AAGCAAGAGT GTGGACAAGA GCCACCTGAA GAGGAAGTTA 
TCTCTGAAGT CAAAGTGGAC GTTAAGGAAT GAAGCAAAGA CTGGGTTCAT GGAGAGCTTT 
CACATCTTCC TCTCAGGCTT GTCATCCAAG GAGTGTAGGA CGTTTATCAT GTTGCTAGCT 
GAACAAAGTG AAGCTTGGGT GCAGGATAAG CAAGATGCAA TCCTTCAGTC CCTCAAGAAA 
CTGGCAGCCA CTCAGCTGAC CGGGCCCAAA GTCATTGAGC TCTGCCATTG CACGTTTGAA 
ACGCAAGGCC TCGAAGTAGC CCAGCACATT GGGAGCCTGC TGAATTTCAA GTACGAGTTT 
AAAAACTTCA GACTGACACC TCTGGACATG TCGGCTTTGG TTTTTGTCAT CAACTCGGGC 
CAGGATGTGA CTCACTTGGA TTTTGCAGGG TGTCCCTTAG ACACGGACTG TTTGGAGATG 
CTGGCCAGCT GTAGGAATGT GGAGCATCTG AGCTTTCGGA GTAGGAGATT TGGTGATGAC 
TTTGCTGCTG CGCTTTCAAA GGGCTTAGGA GAAATGGGGA GCCTGAAGAA ACTAGAGGTG 
ACAGGTGGAA GCATCACAGC TGCGGGGCTG ACTGACATGG TCCAAGCTTT GTCACACTGC 
CTGCAGCTCG AGGAAATAAA CTTGCAGGAC AATCGGATAC AGAATCCAGA CGTAAAGACA 
GTGATGGAGC TGTTTTCCAG AATGGAAAAG CTGAAGAAAA TAGATCTGAG CAAGAACAGT 
CTTTCCTTGA ATGCTGTTCT CCTTTTGGCA AAGGAGTTCA TCGCGTGTCA GAATGCTGCT 
GAGCTACACG TCAGGAAGGA CACTGTGATA ATATCTTTCT CGGGCCCATC TGGGAAAGTT 
CCAAGGTCTT TGGATTTGAA GCGGGAACAA AACAAGGAAT GTGTGACTCC AACTAGACAT 
CTGAGGTTGT GCTTGCAGGC TCGCTGCTTG TCTTCACAAC ATGCAAAGGA GATTGTGTCC 
ATTCTGCAGA GCTGCCCACA CCTCTCTGAA GTAGATTTAT CAGATAACAA GCTTGGGGAT 
GAAGGCTGCA GTTTCCTGCT GGAAAACCTC TCCTGGATTT CAATTTCAAA GCAGCTGAAT 
CTGAGTCACA ACCTGCTGTC TGTTACTGGC ATTTACAGTC TGTTGAAGGC AGTGAATACC 
TGTCAGAGGG TGGTGGAGGT GGAAGTCAGC CTTTATCATA ACACTGCAGT CCTGAGGTTT 
ACAGAAGACG GAGAATTTGC TTCCCCTCCT GCTAGTAGGG AAGAGCCTTT GTACCCTACT 
GATGACCAGT GGGATGACAA AGAAAACCAG ACACCTGTTC CCTCCAAAAA AATAAGATTG 
ACATACTGTC GTTTTCAAGC CAGTGACCTG GAGAAGCTGT GTGCAGTCCT GAAGGAATGT 
GGCAGCATCT CTGAGCTGGA CCTCTCAAAT AATTACCTGG GAGATGAAGG ACTTGCACAG 
CTGTTGCAGT TCCTCCCGAA TCTGAAAACG TTGAGGTCAC TCAAGCTAAA TAACAATCAC 
CTCTCCCTGA GCTCTGTGTT CTGCTTAGCT CAGTCCTTGT GTACACTGGA ACACTTGGAA 
ACAATGGACC TCAGCTTAGG ACGCATGCAG GTGGTTCATC TAACCTTTGG GGACAGAATC 
AGGCTCAGAA GGACCAGCAG ATGGAGAAGA AGTTTCCTGG TACATCCGAA GCACGTAACA 
AATGGACAGT GCTTTCGTCT CAGGAACTGC ACGGTGGGTC CTGAGGACGT GACCAGGCTC 
TGCCAGATGC TGACGCAGTG TACCCAGCTG ACGGAAATCG ATCTGTGTGG AAATGCACTG 
AATGACCAAA GCATTGAGAG ATTACTGAGC TTCCTGCCAC ACTTCTGCCA GCTGACACTA 
CTGAGTATTA GGAACAACAC CTTTTCCCCA TGTTGTGCTG TATTATTTGC CAACTCGATC 
AATCTCTGTG AGCGCATCAG AAGGGTGGAA GTCCGGTCAA GTCAGAATGC TTTTTTACAC 
TTAAGAACAA GCACGCAGAG CCAGAAGACA TCCTGCAGGC TGACTGACTG TGCCATCGGC 
CAAAGGCAGA TAGAGAAGCT CTGCAGAGTC CTGGAGCAGC ATGGGTGTCT GGCAGAACTA 
GATCTCTCCA GGAATCAGCT GGGAGATGAA GTCTTGAGGT TCCTCTTGGA CCACTTGCAT 
CGAGTGCCTG TTACCTGTTC TCTCAATCTC AGCCATAATA GGATTTCTCA GCGTGGAGTT 
CTGCATCTCA TAAATACTTT TACCACTTCT GGAAATACCA CCGAAGTTCA GGTCAGTCTG 
TGCTCCAAGG CAACTTTAAT CATCAAGATG ACAAGCAGAG ATGACCCAAG AAAGATTTTG 
AGGCTCACAG AGTGCAGCTT TCAGCCAGAA CACTTGGAAA AGCTGTGTTT GGTTCTGGAG 
AACTGCACCA ATCTGACAGA GTGCATATCC TCCAACAACA ACGTGACAGT CGATGCTGCA 
GGGGGGCTTT TACGTTCACT GAGTAAAACT CCAGGTCCTC TAAAAATCAG CATAGAAGAG 
CCCTGGGTAT GTAAGTTGAG TGTCACGAGC CTTCTTGAGC TGGCTGTCCA GGCCCATGGA 
AACATCACTG CGATCATGAT ATGCAAAGAG AAGAACCTCT TCCAACTAGG TGTAAGGCTT 
CCACACTGCC TGGAGAAGGT GGGATCAGTC GTTAGCAGAT TGAATTTGAA CGAGCCAGAA 
ATCAAACAGG CTTGCTTCTA CCAAAGGGTT CATGACAAAT GCACCCAGCT TCAGGAACTG 
AGATGGTCTC ATGTTGAACT CCACGATGAC ACAGAAATGC TCGTCAGTAT TTTGCTACCT 
CTTCCAGATC TGAAGAAGTT TGAGCTGACC TCTTGCAGCT TTACACCAAC TGGAATTGAT 
TGCTTGATCA CAGGCTTGCA GAGGTGCCAG GCCATTGAAG AGCTCAACCT GGGCCACATG 
AAGCTCGGCG ATGCTGCCAT TCCTAAGCTA GTGTTTGGAC TCTGTGAAAT GCCGTCTCTG 
AAAAGGCTGA TCTTGAACCA TAACAGTATT GGTGACGATG GCTGCTCCAG ACTCGCAGAA 
GCCCTGAGCA GCATGCACTG CATGGAAGAA ATCAATTTAG GCCACAACAA GATTGGAGAT 
CTAGGCCTGA TAAATATAGC TGCTGTCCTG CTGGAAATGC AAAACTTGAA GAGAATCGAC 
CTCTCAGGGA ACTGTCCCAG TCCTGCTGGA GGAGAAAAGC TGATGGAAGC TCTTGCCAAT 
TGCAAGCACC TCGAGGAGCT AATACTGTCA AGGAATGATT TTGGAGATGG GACAGCAGTG 
AAACTTGCCC TCTGTCTGCC TCACATGAAC AGGCTGAAGA TCCTGCACTT GCAGCACAAT 
AACATCGGGC CAGCAGGAGG AACGGAGCTG GCCAGAGCCC TGATGGCATG TGAACTGTTA 
GAAGAGATAA GTTTATCTGA AAACAACCTT GGGGAAGGAG GTATCCGTGC CCTGTCCGAG 
GGGCTGCCGC GTTTTGAGCA CCTCCGGAAG ATTGAGTTGA GACTCTGTGG AATCACTGAT 
GATGCTTCAA AATTACTCTC TCGTGGCTTC CAGCAATGCC CTGCCGTGGA GGAGATCATA 
TTGTCTTGGA ACGCCCTTGG TGATGGAGGA GCCCAAGTGT TGGCCAGCAT CCTCCCAGGG 
ATGGAAACGC TGAAGATGTT AGACCTGGAG AAAAATCTCA TTGGTGCGTG TGGTGCTACC 
AGGTTGGCAG AGGAACTTGT CAGGTGCCCA GAAATTCAGT TCATAAGGCT GTGGGACAAT 
CCGGTGCCCA AAGGCCTCGC TGAAAGTTTG ACAAGCCAAG ACCCAAGACT GTGCTTCTCC 
TTCTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305364.1
CDS	309..5897
Translation	MQPTLQNDDFNLEGAVASIRPQLVEFLSHRLDWLLSASQHFLPTAVLSGLAGVTDHREKVSVLLDLLEKAGHATWKQFAQCLCMECDLPLEMEILLMSSAGEGNLTQKQEAQTDASDQSSVPRGLTRRRRSSSPISDKGAKQKRLDSAEKYQRLLVDSICKRYGSRRAAGAAAQEPTQPLAFSQAFVNLVLRQSKASRLKERTDKPREDVPSAPEPEECVDTAVRVSDLFGSVVRSGTTKVIFLFGKPGTGKTMLMHRICQKWAEGVLHQFLFTFLFEFRQLNLLKRKLTLKELLFDLFLQPEDSPDAVFQHLLENAQRTLIIFDGLDEFVGNMDVSSASKGSLNRSSSMSISELFAELCHGNLLPGCTVLVTSRPKRVPDFLINTVDLLAEVWGFDHEKVEEYVSHYFRHHSFKEQAIAHLKNNTKLLSMCQIPALCYVVCICLEYLLLKHQTSVELPQTMTQFYIKMLLIFINKQQGEHAGDEEAQLNSNKKAILGLCDLALKGLEAKKLVFYVGDIPEHVKEFASLHGLLTVFEVKTNSAHPETGYAFVHLSLQEFFAALCLMISKSVDKSHLKRKLSLKSKWTLRNEAKTGFMESFHIFLSGLSSKECRTFIMLLAEQSEAWVQDKQDAILQSLKKLAATQLTGPKVIELCHCTFETQGLEVAQHIGSLLNFKYEFKNFRLTPLDMSALVFVINSGQDVTHLDFAGCPLDTDCLEMLASCRNVEHLSFRSRRFGDDFAAALSKGLGEMGSLKKLEVTGGSITAAGLTDMVQALSHCLQLEEINLQDNRIQNPDVKTVMELFSRMEKLKKIDLSKNSLSLNAVLLLAKEFIACQNAAELHVRKDTVIISFSGPSGKVPRSLDLKREQNKECVTPTRHLRLCLQARCLSSQHAKEIVSILQSCPHLSEVDLSDNKLGDEGCSFLLENLSWISISKQLNLSHNLLSVTGIYSLLKAVNTCQRVVEVEVSLYHNTAVLRFTEDGEFASPPASREEPLYPTDDQWDDKENQTPVPSKKIRLTYCRFQASDLEKLCAVLKECGSISELDLSNNYLGDEGLAQLLQFLPNLKTLRSLKLNNNHLSLSSVFCLAQSLCTLEHLETMDLSLGRMQVVHLTFGDRIRLRRTSRWRRSFLVHPKHVTNGQCFRLRNCTVGPEDVTRLCQMLTQCTQLTEIDLCGNALNDQSIERLLSFLPHFCQLTLLSIRNNTFSPCCAVLFANSINLCERIRRVEVRSSQNAFLHLRTSTQSQKTSCRLTDCAIGQRQIEKLCRVLEQHGCLAELDLSRNQLGDEVLRFLLDHLHRVPVTCSLNLSHNRISQRGVLHLINTFTTSGNTTEVQVSLCSKATLIIKMTSRDDPRKILRLTECSFQPEHLEKLCLVLENCTNLTECISSNNNVTVDAAGGLLRSLSKTPGPLKISIEEPWVCKLSVTSLLELAVQAHGNITAIMICKEKNLFQLGVRLPHCLEKVGSVVSRLNLNEPEIKQACFYQRVHDKCTQLQELRWSHVELHDDTEMLVSILLPLPDLKKFELTSCSFTPTGIDCLITGLQRCQAIEELNLGHMKLGDAAIPKLVFGLCEMPSLKRLILNHNSIGDDGCSRLAEALSSMHCMEEINLGHNKIGDLGLINIAAVLLEMQNLKRIDLSGNCPSPAGGEKLMEALANCKHLEELILSRNDFGDGTAVKLALCLPHMNRLKILHLQHNNIGPAGGTELARALMACELLEEISLSENNLGEGGIRALSEGLPRFEHLRKIELRLCGITDDASKLLSRGFQQCPAVEEIILSWNALGDGGAQVLASILPGMETLKMLDLEKNLIGACGATRLAEELVRCPEIQFIRLWDNPVPKGLAESLTSQDPRLCFSFC

Target ORF information:

RefSeq Version	NM_001318435.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NLR family CARD domain containing 5 (NLRC5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318435.1

ORF Insert Sequence:

ATGCAGCCCA CACTCCAGAA CGATGACTTC AACCTGGAAG GAGCTGTAGC AAGCATCCGC 
CCCCAGCTAG TGGAGTTCCT CAGCCATCGT CTCGACTGGC TGCTCTCTGC TTCCCAGCAC 
TTCCTCCCTA CGGCTGTCCT CAGCGGCTTG GCAGGCGTCA CTGACCACAG AGAGAAAGTC 
TCTGTGCTCC TCGACCTGCT GGAGAAGGCT GGCCATGCCA CCTGGAAACA GTTTGCACAG 
TGCCTCTGCA TGGAGTGTGA CCTGCCCCTG GAAATGGAGA TACTGCTCAT GAGTTCTGCA 
GGAGAAGGTA ACTTGACTCA GAAGCAAGAA GCTCAGACAG ACGCAAGTGA CCAGTCCTCT 
GTGCCAAGAG GGTTGACGCG CCGCCGCCGT AGCAGCAGCC CCATCAGTGA TAAGGGTGCT 
AAACAGAAAC GCCTGGACTC AGCTGAAAAG TATCAACGTC TTCTCGTTGA CTCCATCTGC 
AAGAGATATG GAAGCAGGAG AGCAGCAGGA GCAGCGGCAC AAGAGCCAAC GCAGCCTCTG 
GCTTTCAGCC AAGCCTTTGT CAACCTCGTT CTTCGGCAGA GCAAGGCCTC CCGACTGAAA 
GAGAGAACAG ACAAACCCCG GGAGGACGTG CCCAGTGCCC CCGAGCCAGA AGAATGTGTG 
GATACAGCCG TGAGAGTGTC AGATCTCTTT GGCAGCGTGG TCCGATCGGG TACAACGAAG 
GTGATCTTTT TGTTTGGTAA ACCAGGTACA GGCAAGACCA TGCTGATGCA CAGGATTTGT 
CAGAAGTGGG CGGAAGGTGT CTTACATCAG TTTCTCTTCA CTTTCCTGTT TGAGTTCCGG 
CAGTTGAATT TACTAAAAAG AAAACTGACT CTGAAGGAGC TTTTGTTTGA CCTGTTTCTT 
CAGCCAGAAG ACAGCCCCGA TGCAGTGTTC CAGCACCTCC TGGAAAACGC CCAACGTACA 
CTGATCATCT TTGATGGGCT GGATGAGTTT GTGGGTAACA TGGACGTGTC ATCAGCTTCT 
AAAGGAAGCC TGAACCGTAG CTCCAGTATG TCCATATCTG AACTCTTTGC AGAGCTTTGT 
CATGGGAACC TCCTACCTGG CTGCACGGTG TTGGTCACCA GCCGCCCTAA GAGAGTCCCT 
GACTTCTTGA TAAACACAGT AGATTTGCTG GCAGAAGTTT GGGGGTTTGA TCACGAGAAG 
GTTGAGGAGT ATGTCAGCCA CTATTTTCGT CATCATTCAT TCAAAGAACA AGCCATCGCT 
CACCTGAAGA ACAACACCAA GCTCCTCAGC ATGTGCCAGA TCCCTGCCCT GTGTTACGTT 
GTCTGCATCT GTTTGGAGTA TTTGCTCCTT AAGCATCAGA CGAGCGTGGA GCTTCCCCAG 
ACCATGACAC AGTTTTATAT CAAAATGCTT CTCATCTTCA TAAACAAACA GCAGGGGGAG 
CATGCAGGTG ATGAGGAGGC TCAGCTGAAC AGTAACAAGA AGGCCATTTT GGGTCTGTGT 
GATCTTGCAC TGAAAGGCCT GGAAGCTAAG AAGCTCGTGT TTTACGTTGG AGATATTCCA 
GAGCACGTGA AAGAGTTTGC TTCCTTGCAC GGTCTGCTGA CTGTCTTTGA GGTGAAGACA 
AACAGCGCTC ACCCCGAGAC TGGCTATGCC TTTGTGCACT TGAGCCTGCA GGAGTTTTTT 
GCAGCCCTGT GTCTCATGAT AAGCAAGAGT GTGGACAAGA GCCACCTGAA GAGGAAGTTA 
TCTCTGAAGT CAAAGTGGAC GTTAAGGAAT GAAGCAAAGA CTGGGTTCAT GGAGAGCTTT 
CACATCTTCC TCTCAGGCTT GTCATCCAAG GAGTGTAGGA CGTTTATCAT GTTGCTAGCT 
GAACAAAGTG AAGCTTGGGT GCAGGATAAG CAAGATGCAA TCCTTCAGTC CCTCAAGAAA 
CTGGCAGCCA CTCAGCTGAC CGGGCCCAAA GTCATTGAGC TCTGCCATTG CACGTTTGAA 
ACGCAAGGCC TCGAAGTAGC CCAGCACATT GGGAGCCTGC TGAATTTCAA GTACGAGTTT 
AAAAACTTCA GACTGACACC TCTGGACATG TCGGCTTTGG TTTTTGTCAT CAACTCGGGC 
CAGGATGTGA CTCACTTGGA TTTTGCAGGG TGTCCCTTAG ACACGGACTG TTTGGAGATG 
CTGGCCAGCT GTAGGAATGT GGAGCATCTG AGCTTTCGGA GTAGGAGATT TGGTGATGAC 
TTTGCTGCTG CGCTTTCAAA GGGCTTAGGA GAAATGGGGA GCCTGAAGAA ACTAGAGGTG 
ACAGGTGGAA GCATCACAGC TGCGGGGCTG ACTGACATGG TCCAAGCTTT GTCACACTGC 
CTGCAGCTCG AGGAAATAAA CTTGCAGGAC AATCGGATAC AGAATCCAGA CGTAAAGACA 
GTGATGGAGC TGTTTTCCAG AATGGAAAAG CTGAAGAAAA TAGATCTGAG CAAGAACAGT 
CTTTCCTTGA ATGCTGTTCT CCTTTTGGCA AAGGAGTTCA TCGCGTGTCA GAATGCTGCT 
GAGCTACACG TCAGGAAGGA CACTGTGATA ATATCTTTCT CGGGCCCATC TGGGAAAGTT 
CCAAGGTCTT TGGATTTGAA GCGGGAACAA AACAAGGAAT GTGTGACTCC AACTAGACAT 
CTGAGGTTGT GCTTGCAGGC TCGCTGCTTG TCTTCACAAC ATGCAAAGGA GATTGTGTCC 
ATTCTGCAGA GCTGCCCACA CCTCTCTGAA GTAGATTTAT CAGATAACAA GCTTGGGGAT 
GAAGGCTGCA GTTTCCTGCT GGAAAACCTC TCCTGGATTT CAATTTCAAA GCAGCTGAAT 
CTGAGTCACA ACCTGCTGTC TGTTACTGGC ATTTACAGTC TGTTGAAGGC AGTGAATACC 
TGTCAGAGGG TGGTGGAGGT GGAAGTCAGC CTTTATCATA ACACTGCAGT CCTGAGGTTT 
ACAGAAGACG GAGAATTTGC TTCCCCTCCT GCTAGTAGGG AAGAGCCTTT GTACCCTACT 
GATGACCAGT GGGATGACAA AGAAAACCAG ACACCTGTTC CCTCCAAAAA AATAAGATTG 
ACATACTGTC GTTTTCAAGC CAGTGACCTG GAGAAGCTGT GTGCAGTCCT GAAGGAATGT 
GGCAGCATCT CTGAGCTGGA CCTCTCAAAT AATTACCTGG GAGATGAAGG ACTTGCACAG 
CTGTTGCAGT TCCTCCCGAA TCTGAAAACG TTGAGGTCAC TCAAGCTAAA TAACAATCAC 
CTCTCCCTGA GCTCTGTGTT CTGCTTAGCT CAGTCCTTGT GTACACTGGA ACACTTGGAA 
ACAATGGACC TCAGCTTAGG ACGCATGCAG GTGGTTCATC TAACCTTTGG GGACAGAATC 
AGGCTCAGAA GGACCAGCAG ATGGAGAAGA AGTTTCCTGG TACATCCGAA GCACGTAACA 
AATGGACAGT GCTTTCGTCT CAGGAACTGC ACGGTGGGTC CTGAGGACGT GACCAGGCTC 
TGCCAGATGC TGACGCAGTG TACCCAGCTG ACGGAAATCG ATCTGTGTGG AAATGCACTG 
AATGACCAAA GCATTGAGAG ATTACTGAGC TTCCTGCCAC ACTTCTGCCA GCTGACACTA 
CTGAGTATTA GGAACAACAC CTTTTCCCCA TGTTGTGCTG TATTATTTGC CAACTCGATC 
AATCTCTGTG AGCGCATCAG AAGGGTGGAA GTCCGGTCAA GTCAGAATGC TTTTTTACAC 
TTAAGAACAA GCACGCAGAG CCAGAAGACA TCCTGCAGGC TGACTGACTG TGCCATCGGC 
CAAAGGCAGA TAGAGAAGCT CTGCAGAGTC CTGGAGCAGC ATGGGTGTCT GGCAGAACTA 
GATCTCTCCA GGAATCAGCT GGGAGATGAA GTCTTGAGGT TCCTCTTGGA CCACTTGCAT 
CGAGTGCCTG TTACCTGTTC TCTCAATCTC AGCCATAATA GGATTTCTCA GCGTGGAGTT 
CTGCATCTCA TAAATACTTT TACCACTTCT GGAAATACCA CCGAAGTTCA GGTCAGTCTG 
TGCTCCAAGG CAACTTTAAT CATCAAGATG ACAAGCAGAG ATGACCCAAG AAAGATTTTG 
AGGCTCACAG AGTGCAGCTT TCAGCCAGAA CACTTGGAAA AGCTGTGTTT GGTTCTGGAG 
AACTGCACCA ATCTGACAGA GTGCATATCC TCCAACAACA ACGTGACAGT CGATGCTGCA 
GGGGGGCTTT TACGTTCACT GAGTAAAACT CCAGGTCCTC TAAAAATCAG CATAGAAGAG 
CCCTGGGTAT GTAAGTTGAG TGTCACGAGC CTTCTTGAGC TGGCTGTCCA GGCCCATGGA 
AACATCACTG CGATCATGAT ATGCAAAGAG AAGAACCTCT TCCAACTAGG TGTAAGGCTT 
CCACACTGCC TGGAGAAGGT GGGATCAGTC GTTAGCAGAT TGAATTTGAA CGAGCCAGAA 
ATCAAACAGG CTTGCTTCTA CCAAAGGGTT CATGACAAAT GCACCCAGCT TCAGGAACTG 
AGATGGTCTC ATGTTGAACT CCACGATGAC ACAGAAATGC TCGTCAGTAT TTTGCTACCT 
CTTCCAGATC TGAAGAAGTT TGAGCTGACC TCTTGCAGCT TTACACCAAC TGGAATTGAT 
TGCTTGATCA CAGGCTTGCA GAGGTGCCAG GCCATTGAAG AGCTCAACCT GGGCCACATG 
AAGCTCGGCG ATGCTGCCAT TCCTAAGCTA GTGTTTGGAC TCTGTGAAAT GCCGTCTCTG 
AAAAGGCTGA TCTTGAACCA TAACAGTATT GGTGACGATG GCTGCTCCAG ACTCGCAGAA 
GCCCTGAGCA GCATGCACTG CATGGAAGAA ATCAATTTAG GCCACAACAA GATTGGAGAT 
CTAGGCCTGA TAAATATAGC TGCTGTCCTG CTGGAAATGC AAAACTTGAA GAGAATCGAC 
CTCTCAGGGA ACTGTCCCAG TCCTGCTGGA GGAGAAAAGC TGATGGAAGC TCTTGCCAAT 
TGCAAGCACC TCGAGGAGCT AATACTGTCA AGGAATGATT TTGGAGATGG GACAGCAGTG 
AAACTTGCCC TCTGTCTGCC TCACATGAAC AGGCTGAAGA TCCTGCACTT GCAGCACAAT 
AACATCGGGC CAGCAGGAGG AACGGAGCTG GCCAGAGCCC TGATGGCATG TGAACTGTTA 
GAAGAGATAA GTTTATCTGA AAACAACCTT GGGGAAGGAG GTATCCGTGC CCTGTCCGAG 
GGGCTGCCGC GTTTTGAGCA CCTCCGGAAG ATTGAGTTGA GACTCTGTGG AATCACTGAT 
GATGCTTCAA AATTACTCTC TCGTGGCTTC CAGCAATGCC CTGCCGTGGA GGAGATCATA 
TTGTCTTGGA ACGCCCTTGG TGATGGAGGA GCCCAAGTGT TGGCCAGCAT CCTCCCAGGG 
ATGGAAACGC TGAAGATGTT AGACCTGGAG AAAAATCTCA TTGGTGCGTG TGGTGCTACC 
AGGTTGGCAG AGGAACTTGT CAGGTGCCCA GAAATTCAGT TCATAAGGCT GTGGGACAAT 
CCGGTGCCCA AAGGCCTCGC TGAAAGTTTG ACAAGCCAAG ACCCAAGACT GTGCTTCTCC 
TTCTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRC5 ( XM_004944054.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004944111.1 Gallus gallus NLR family, CARD domain containing 5 (NLRC5), transcript variant X2, mRNA.

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CloneID	OGa13511
Clone ID Related Accession (Same CDS sequence)	XM_004944054.2 , XM_015292328.1 , XM_015292327.1
Accession Version	XM_004944054.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	protein NLRC5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455926.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004944054.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCA CACTCCAGAA CGATGACTTC AACCTGGAAG GAGCTGTAGC AAGCATCCGC 
CCCCAGCTAG TGGAGTTCCT CAGCCATCGT CTCGACTGGC TGCTCTCTGC TTCCCAGCAC 
TTCCTCCCTA CGGCTGTCCT CAGCGGCTTG GCAGGCGTCA CTGACCACAG AGAGAAAGTC 
TCTGTGCTCC TCGACCTGCT GGAGAAGGCT GGCCATGCCA CCTGGAAACA GTTTGCACAG 
TGCCTCTGCA TGGAGTGTGA CCTGCCCCTG GAAATGGAGA TACTGCTCAT GAGTTCTGCA 
GGAGAAGGTA ACTTGACTCA GAAGCAAGAA GCTCAGACAG ACGCAAGTGA CCAGTCCTCT 
GTGCCAAGAG GGTTGACGCG CCGCCGCCGT AGCAGCAGCC CCATCAGTGA TAAGGGTGCT 
AAACAGAAAC GCCTGGACTC AGCTGAAAAG TATCAACGTC TTCTCGTTGA CTCCATCTGC 
AAGAGATATG GAAGCAGGAG AGCAGCAGGA GCAGCGGCAC AAGAGCCAAC GCAGCCTCTG 
GCTTTCAGCC AAGCCTTTGT CAACCTCGTT CTTCGGCAGA GCAAGGCCTC CCGACTGAAA 
GAGAGAACAG ACAAACCCCG GGAGGACGTG CCCAGTGCCC CCGAGCCAGA AGAATGTGTG 
GATACAGCCG TGAGAGTGTC AGATCTCTTT GGCAGCGTGG TCCGATCGGG TACAACGAAG 
GTGATCTTTT TGTTTGGTAA ACCAGGTACA GGCAAGACCA TGCTGATGCA CAGGATTTGT 
CAGAAGTGGG CGGAAGGTGT CTTACATCAG TTTCTCTTCA CTTTCCTGTT TGAGTTCCGG 
CAGTTGAATT TACTAAAAAG AAAACTGACT CTGAAGGAGC TTTTGTTTGA CCTGTTTCTT 
CAGCCAGAAG ACAGCCCCGA TGCAGTGTTC CAGCACCTCC TGGAAAACGC CCAACGTACA 
CTGATCATCT TTGATGGGCT GGATGAGTTT GTGGGTAACA TGGACGTGTC ATCAGCTTCT 
AAAGGAAGCC TGAACCGTAG CTCCAGTATG TCCATATCTG AACTCTTTGC AGAGCTTTGT 
CATGGGAACC TCCTACCTGG CTGCACGGTG TTGGTCACCA GCCGCCCTAA GAGAGTCCCT 
GACTTCTTGA TAAACACAGT AGATTTGCTG GCAGAAGTTT GGGGGTTTGA TCACGAGAAG 
GTTGAGGAGT ATGTCAGCCA CTATTTTCGT CATCATTCAT TCAAAGAACA AGCCATCGCT 
CACCTGAAGA ACAACACCAA GCTCCTCAGC ATGTGCCAGA TCCCTGCCCT GTGTTACGTT 
GTCTGCATCT GTTTGGAGTA TTTGCTCCTT AAGCATCAGA CGAGCGTGGA GCTTCCCCAG 
ACCATGACAC AGTTTTATAT CAAAATGCTT CTCATCTTCA TAAACAAACA GCAGGGGGAG 
CATGCAGGTG ATGAGGAGGC TCAGCTGAAC AGTAACAAGA AGGCCATTTT GGGTCTGTGT 
GATCTTGCAC TGAAAGGCCT GGAAGCTAAG AAGCTCGTGT TTTACGTTGG AGATATTCCA 
GAGCACGTGA AAGAGTTTGC TTCCTTGCAC GGTCTGCTGA CTGTCTTTGA GGTGAAGACA 
AACAGCGCTC ACCCCGAGAC TGGCTATGCC TTTGTGCACT TGAGCCTGCA GGAGTTTTTT 
GCAGCCCTGT GTCTCATGAT AAGCAAGAGT GTGGACAAGA GCCACCTGAA GAGGAAGTTA 
TCTCTGAAGT CAAAGTGGAC GTTAAGGAAT GAAGCAAAGA CTGGGTTCAT GGAGAGCTTT 
CACATCTTCC TCTCAGGCTT GTCATCCAAG GAGTGTAGGA CGTTTATCAT GTTGCTAGCT 
GAACAAAGTG AAGCTTGGGT GCAGGATAAG CAAGATGCAA TCCTTCAGTC CCTCAAGAAA 
CTGGCAGCCA CTCAGCTGAC CGGGCCCAAA GTCATTGAGC TCTGCCATTG CACGTTTGAA 
ACGCAAGGCC TCGAAGTAGC CCAGCACATT GGGAGCCTGC TGAATTTCAA GTACGAGTTT 
AAAAACTTCA GACTGACACC TCTGGACATG TCGGCTTTGG TTTTTGTCAT CAACTCGGGC 
CAGGATGTGA CTCACTTGGA TTTTGCAGGG TGTCCCTTAG ACACGGACTG TTTGGAGATG 
CTGGCCAGCT GTAGGAATGT GGAGCATCTG AGCTTTCGGA GTAGGAGATT TGGTGATGAC 
TTTGCTGCTG CGCTTTCAAA GGGCTTAGGA GAAATGGGGA GCCTGAAGAA ACTAGAGGTG 
ACAGGTGGAA GCATCACAGC TGCGGGGCTG ACTGACATGG TCCAAGCTTT GTCACACTGC 
CTGCAGCTCG AGGAAATAAA CTTGCAGGAC AATCGGATAC AGAATCCAGA CGTAAAGACA 
GTGATGGAGC TGTTTTCCAG AATGGAAAAG CTGAAGAAAA TAGATCTGAG CAAGAACAGT 
CTTTCCTTGA ATGCTGTTCT CCTTTTGGCA AAGGAGTTCA TCGCGTGTCA GAATGCTGCT 
GAGCTACACG TCAGGAAGGA CACTGTGATA ATATCTTTCT CGGGCCCATC TGGGAAAGTT 
CCAAGGTCTT TGGATTTGAA GCGGGAACAA AACAAGGAAT GTGTGACTCC AACTAGACAT 
CTGAGGTTGT GCTTGCAGGC TCGCTGCTTG TCTTCACAAC ATGCAAAGGA GATTGTGTCC 
ATTCTGCAGA GCTGCCCACA CCTCTCTGAA GTAGATTTAT CAGATAACAA GCTTGGGGAT 
GAAGGCTGCA GTTTCCTGCT GGAAAACCTC TCCTGGATTT CAATTTCAAA GCAGCTGAAT 
CTGAGTCACA ACCTGCTGTC TGTTACTGGC ATTTACAGTC TGTTGAAGGC AGTGAATACC 
TGTCAGAGGG TGGTGGAGGT GGAAGTCAGC CTTTATCATA ACACTGCAGT CCTGAGGTTT 
ACAGAAGATG GAGAATTTGC TTCCCCTCCT GCTAGTAGGG AAGAGCCTTT GTACCCTACT 
GATGACCAGT GGGATGACAA AGAAAACCAG ACACCTGTTC CCTCCAAAAA AATAAGATTG 
ACATACTGTC GTTTTCAAGC CAGTGACCTG GAGAAGCTGT GTGCAGTCCT GAAGGAATGT 
GGCAGCATCT CTGAGCTGGA CCTCTCAAAT AATTACCTGG GAGATGAAGG ACTTGCACAG 
CTGTTGCAGT TCCTCCCGAA TCTGAAAACG TTGAGGTCAC TCAAGCTAAA TAACAATCAC 
CTCTCCCTGA GCTCTGTGTT CTGCTTAGCT CAGTCCTTGT GTACACTGGA ACACTTGGAA 
ACAATGGACC TCAGCTTAGG ACGCATGCAG GTGGTTCATC TAACCTTTGG GGACAGAATC 
AGGCTCAGAA GGACCAGCAG ATGGAGAAGT TGTTTCCTGG TGCATCCGAA GCACGTAACA 
AATGGACAGT GCTTTCGTCT CAGGAACTGC ACGGTGGGTC CTGAGGACGT GACCAGGCTC 
TGCCAGATGC TGATGCAGTG TACCCAGCTG ACGGAAATCG ATCTGTGTGG AAATGCACTG 
AATGACCAAA GCATTGAGAG ATTACTGAGC TTCCTGCCAC ACTTCTGCCA GCTGACACTA 
CTGAGTATTA GGAACAACAC CTTTTCCCCA TGTTGTGCTG TATTATTTGC CAACTCGATC 
AATCTCTGTG AGCGCATCAG AAGGGTGGAA GTCCGGTCAA GTCAGAATGC TTTTTTACAC 
TTAAGAACAA GCACGCAGGG CCAGAAGACA TCCTGCAGGC TGACTGACTG TGCCATCGGC 
CAAAGGCAGA TAGAGAAGCT CTGCAGAGTC CTGGAGCAGC ATGGGTGTCT GGCAGAACTA 
GATCTCTCCA GGAATCAGCT GGGAGATGAA GTCTTGAGGT TCCTCTTGGA CCACTTGCAT 
CGAGTGCCTG TTACCTGTTC TCTCAATCTC AGCCATAATA GGATTTCTCA GCGTGGAGTT 
CTGCATCTCA TAAATACTTT TACCACTTCT GGAAATACCA CCGAAGTCCA GGTCAGTCTG 
TGCTCCAAGG CAACTTTAAT CATCAAGATG ACAAGCAGAG ATGACCCAAG AAAGATTTTG 
AGGCTCACAG AGTGCAGCTT TCAGCCAGAA CACTTGGAAA AGCTATGTTT GGTTCTGGAG 
AACTGCACCA ATCTGACAGA GTGCATATCC TCCAACAACA ACGTGACAGT CGATGCTGCA 
GGGGGGCTTT TACGTTCACT GAGTAAAACT CCAGGTCCTC TAAAAATCAG CATAGAAGAG 
CCCTGGGTAT GTAAGTTGAG TGTCACGAGC CTTCTTGAGC TGGCTGTCCA GGCCCATGGA 
AACATCACTG CGATCATGAT ATGCAAAGAG AAGAACCTCT TCCAACTAGG TGTAAGGCTT 
CCACACTGCC TGGAGAAGGT GGGATCAGTC GTTAGCAGAT TGAATTTGAA CGAGCCAGAA 
ATCAAACAGG CTTGCTTCTA CCAAAGGGTT CATGACAAAT GCACCCAGCT TCAGGAACTG 
AGATGGTCTC ACGTTGAACT CCACGATGAC ACAGAAATGC TCGTCAGTAT TTTGCTACCT 
CTTCCAGATC TGAAGAAGTT TGAGCTGACC TCTTGCAGCT TTACACCAAC TGGAATTGAT 
TGCTTGATCA CAGGCTTGCA GAGGTGCCAG GCCATTGAAG AGCTCAACCT GGGCCACATG 
AAGCTCGGCG ATGCTGCCAT TCCTAAGCTA GTGTTTGGAC TCTGTGAAAT GCCGTCTCTG 
AAAAGGCTGA TCTTGAACCA TAACAGTATT GGTGACGATG GCTGCTCCAG ACTCGCAGAA 
GCCCTGAGCA GCATGCACTG CATGGAAGAA ATCAATTTAG GCCACAACAA GATTGGAGAT 
CTAGGCCTGA TAAATATAGC TGCTGTCCTG CTGGAAATGC AAAACTTGAA GAGAATCGAC 
CTCTCAGGGA ACTGTCCCAG TCCTGCTGGA GGAGAAAAGC TGATGGAAGC TCTTGCCAAT 
TGCAAGCACC TCGAGGAGCT AATACTGTCA AGGAATGATT TTGGAGATGG GACAGCAGTG 
AAACTTGCCC TCTGTCTGCC TCACATGAAC AGGCTGAAGA TCCTGCACTT GCAGCACAAT 
AACATCGGGC CAGCAGGAGG AACGGAGCTG GCCAGAGCCC TGATGGCATG TGAACTGTTA 
GAAGAGATAA GTTTATCTGA AAACAACCTT GGGGAAGGAG GTATCCGTGC CCTGTCCGAG 
GGGCTGCCGC GTTTTGAGCA CCTCCGGAAG ATTGAGTTGA GACTCTGTGG AATCACTGAT 
GATGCTTCAA AATTACTCTC TCGTGGCTTC CAGCAATGCC CTGCTGTGGA GGAGATCATA 
TTGTCTTGGA ACGCCCTTGG TGATGGAGGA GCCCAAGTGT TGGCCAGCAT CCTCCCAGGG 
ATGGAAACGC TGAAGATGTT AGACCTGGAG AAAAATCTCA TTGGTGCGTG TGGTGCTACC 
AGGTTGGCAG AGGAACTTGT CAGGTGCCCA GAAATTCAGT TCATAAGGCT GTGGGACAAT 
CCGGTGCCCA AAGGCCTCGC TGAAAGTTTG ACAAGCCAAG ACCCAAGACT GTGCTTCTCC 
TTCTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004944111.1
CDS	281..5869
Translation	MQPTLQNDDFNLEGAVASIRPQLVEFLSHRLDWLLSASQHFLPTAVLSGLAGVTDHREKVSVLLDLLEKAGHATWKQFAQCLCMECDLPLEMEILLMSSAGEGNLTQKQEAQTDASDQSSVPRGLTRRRRSSSPISDKGAKQKRLDSAEKYQRLLVDSICKRYGSRRAAGAAAQEPTQPLAFSQAFVNLVLRQSKASRLKERTDKPREDVPSAPEPEECVDTAVRVSDLFGSVVRSGTTKVIFLFGKPGTGKTMLMHRICQKWAEGVLHQFLFTFLFEFRQLNLLKRKLTLKELLFDLFLQPEDSPDAVFQHLLENAQRTLIIFDGLDEFVGNMDVSSASKGSLNRSSSMSISELFAELCHGNLLPGCTVLVTSRPKRVPDFLINTVDLLAEVWGFDHEKVEEYVSHYFRHHSFKEQAIAHLKNNTKLLSMCQIPALCYVVCICLEYLLLKHQTSVELPQTMTQFYIKMLLIFINKQQGEHAGDEEAQLNSNKKAILGLCDLALKGLEAKKLVFYVGDIPEHVKEFASLHGLLTVFEVKTNSAHPETGYAFVHLSLQEFFAALCLMISKSVDKSHLKRKLSLKSKWTLRNEAKTGFMESFHIFLSGLSSKECRTFIMLLAEQSEAWVQDKQDAILQSLKKLAATQLTGPKVIELCHCTFETQGLEVAQHIGSLLNFKYEFKNFRLTPLDMSALVFVINSGQDVTHLDFAGCPLDTDCLEMLASCRNVEHLSFRSRRFGDDFAAALSKGLGEMGSLKKLEVTGGSITAAGLTDMVQALSHCLQLEEINLQDNRIQNPDVKTVMELFSRMEKLKKIDLSKNSLSLNAVLLLAKEFIACQNAAELHVRKDTVIISFSGPSGKVPRSLDLKREQNKECVTPTRHLRLCLQARCLSSQHAKEIVSILQSCPHLSEVDLSDNKLGDEGCSFLLENLSWISISKQLNLSHNLLSVTGIYSLLKAVNTCQRVVEVEVSLYHNTAVLRFTEDGEFASPPASREEPLYPTDDQWDDKENQTPVPSKKIRLTYCRFQASDLEKLCAVLKECGSISELDLSNNYLGDEGLAQLLQFLPNLKTLRSLKLNNNHLSLSSVFCLAQSLCTLEHLETMDLSLGRMQVVHLTFGDRIRLRRTSRWRSCFLVHPKHVTNGQCFRLRNCTVGPEDVTRLCQMLMQCTQLTEIDLCGNALNDQSIERLLSFLPHFCQLTLLSIRNNTFSPCCAVLFANSINLCERIRRVEVRSSQNAFLHLRTSTQGQKTSCRLTDCAIGQRQIEKLCRVLEQHGCLAELDLSRNQLGDEVLRFLLDHLHRVPVTCSLNLSHNRISQRGVLHLINTFTTSGNTTEVQVSLCSKATLIIKMTSRDDPRKILRLTECSFQPEHLEKLCLVLENCTNLTECISSNNNVTVDAAGGLLRSLSKTPGPLKISIEEPWVCKLSVTSLLELAVQAHGNITAIMICKEKNLFQLGVRLPHCLEKVGSVVSRLNLNEPEIKQACFYQRVHDKCTQLQELRWSHVELHDDTEMLVSILLPLPDLKKFELTSCSFTPTGIDCLITGLQRCQAIEELNLGHMKLGDAAIPKLVFGLCEMPSLKRLILNHNSIGDDGCSRLAEALSSMHCMEEINLGHNKIGDLGLINIAAVLLEMQNLKRIDLSGNCPSPAGGEKLMEALANCKHLEELILSRNDFGDGTAVKLALCLPHMNRLKILHLQHNNIGPAGGTELARALMACELLEEISLSENNLGEGGIRALSEGLPRFEHLRKIELRLCGITDDASKLLSRGFQQCPAVEEIILSWNALGDGGAQVLASILPGMETLKMLDLEKNLIGACGATRLAEELVRCPEIQFIRLWDNPVPKGLAESLTSQDPRLCFSFC

Target ORF information:

RefSeq Version	XM_004944054.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NLR family, CARD domain containing 5 (NLRC5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004944054.2

ORF Insert Sequence:

ATGCAGCCCA CACTCCAGAA CGATGACTTC AACCTGGAAG GAGCTGTAGC AAGCATCCGC 
CCCCAGCTAG TGGAGTTCCT CAGCCATCGT CTCGACTGGC TGCTCTCTGC TTCCCAGCAC 
TTCCTCCCTA CGGCTGTCCT CAGCGGCTTG GCAGGCGTCA CTGACCACAG AGAGAAAGTC 
TCTGTGCTCC TCGACCTGCT GGAGAAGGCT GGCCATGCCA CCTGGAAACA GTTTGCACAG 
TGCCTCTGCA TGGAGTGTGA CCTGCCCCTG GAAATGGAGA TACTGCTCAT GAGTTCTGCA 
GGAGAAGGTA ACTTGACTCA GAAGCAAGAA GCTCAGACAG ACGCAAGTGA CCAGTCCTCT 
GTGCCAAGAG GGTTGACGCG CCGCCGCCGT AGCAGCAGCC CCATCAGTGA TAAGGGTGCT 
AAACAGAAAC GCCTGGACTC AGCTGAAAAG TATCAACGTC TTCTCGTTGA CTCCATCTGC 
AAGAGATATG GAAGCAGGAG AGCAGCAGGA GCAGCGGCAC AAGAGCCAAC GCAGCCTCTG 
GCTTTCAGCC AAGCCTTTGT CAACCTCGTT CTTCGGCAGA GCAAGGCCTC CCGACTGAAA 
GAGAGAACAG ACAAACCCCG GGAGGACGTG CCCAGTGCCC CCGAGCCAGA AGAATGTGTG 
GATACAGCCG TGAGAGTGTC AGATCTCTTT GGCAGCGTGG TCCGATCGGG TACAACGAAG 
GTGATCTTTT TGTTTGGTAA ACCAGGTACA GGCAAGACCA TGCTGATGCA CAGGATTTGT 
CAGAAGTGGG CGGAAGGTGT CTTACATCAG TTTCTCTTCA CTTTCCTGTT TGAGTTCCGG 
CAGTTGAATT TACTAAAAAG AAAACTGACT CTGAAGGAGC TTTTGTTTGA CCTGTTTCTT 
CAGCCAGAAG ACAGCCCCGA TGCAGTGTTC CAGCACCTCC TGGAAAACGC CCAACGTACA 
CTGATCATCT TTGATGGGCT GGATGAGTTT GTGGGTAACA TGGACGTGTC ATCAGCTTCT 
AAAGGAAGCC TGAACCGTAG CTCCAGTATG TCCATATCTG AACTCTTTGC AGAGCTTTGT 
CATGGGAACC TCCTACCTGG CTGCACGGTG TTGGTCACCA GCCGCCCTAA GAGAGTCCCT 
GACTTCTTGA TAAACACAGT AGATTTGCTG GCAGAAGTTT GGGGGTTTGA TCACGAGAAG 
GTTGAGGAGT ATGTCAGCCA CTATTTTCGT CATCATTCAT TCAAAGAACA AGCCATCGCT 
CACCTGAAGA ACAACACCAA GCTCCTCAGC ATGTGCCAGA TCCCTGCCCT GTGTTACGTT 
GTCTGCATCT GTTTGGAGTA TTTGCTCCTT AAGCATCAGA CGAGCGTGGA GCTTCCCCAG 
ACCATGACAC AGTTTTATAT CAAAATGCTT CTCATCTTCA TAAACAAACA GCAGGGGGAG 
CATGCAGGTG ATGAGGAGGC TCAGCTGAAC AGTAACAAGA AGGCCATTTT GGGTCTGTGT 
GATCTTGCAC TGAAAGGCCT GGAAGCTAAG AAGCTCGTGT TTTACGTTGG AGATATTCCA 
GAGCACGTGA AAGAGTTTGC TTCCTTGCAC GGTCTGCTGA CTGTCTTTGA GGTGAAGACA 
AACAGCGCTC ACCCCGAGAC TGGCTATGCC TTTGTGCACT TGAGCCTGCA GGAGTTTTTT 
GCAGCCCTGT GTCTCATGAT AAGCAAGAGT GTGGACAAGA GCCACCTGAA GAGGAAGTTA 
TCTCTGAAGT CAAAGTGGAC GTTAAGGAAT GAAGCAAAGA CTGGGTTCAT GGAGAGCTTT 
CACATCTTCC TCTCAGGCTT GTCATCCAAG GAGTGTAGGA CGTTTATCAT GTTGCTAGCT 
GAACAAAGTG AAGCTTGGGT GCAGGATAAG CAAGATGCAA TCCTTCAGTC CCTCAAGAAA 
CTGGCAGCCA CTCAGCTGAC CGGGCCCAAA GTCATTGAGC TCTGCCATTG CACGTTTGAA 
ACGCAAGGCC TCGAAGTAGC CCAGCACATT GGGAGCCTGC TGAATTTCAA GTACGAGTTT 
AAAAACTTCA GACTGACACC TCTGGACATG TCGGCTTTGG TTTTTGTCAT CAACTCGGGC 
CAGGATGTGA CTCACTTGGA TTTTGCAGGG TGTCCCTTAG ACACGGACTG TTTGGAGATG 
CTGGCCAGCT GTAGGAATGT GGAGCATCTG AGCTTTCGGA GTAGGAGATT TGGTGATGAC 
TTTGCTGCTG CGCTTTCAAA GGGCTTAGGA GAAATGGGGA GCCTGAAGAA ACTAGAGGTG 
ACAGGTGGAA GCATCACAGC TGCGGGGCTG ACTGACATGG TCCAAGCTTT GTCACACTGC 
CTGCAGCTCG AGGAAATAAA CTTGCAGGAC AATCGGATAC AGAATCCAGA CGTAAAGACA 
GTGATGGAGC TGTTTTCCAG AATGGAAAAG CTGAAGAAAA TAGATCTGAG CAAGAACAGT 
CTTTCCTTGA ATGCTGTTCT CCTTTTGGCA AAGGAGTTCA TCGCGTGTCA GAATGCTGCT 
GAGCTACACG TCAGGAAGGA CACTGTGATA ATATCTTTCT CGGGCCCATC TGGGAAAGTT 
CCAAGGTCTT TGGATTTGAA GCGGGAACAA AACAAGGAAT GTGTGACTCC AACTAGACAT 
CTGAGGTTGT GCTTGCAGGC TCGCTGCTTG TCTTCACAAC ATGCAAAGGA GATTGTGTCC 
ATTCTGCAGA GCTGCCCACA CCTCTCTGAA GTAGATTTAT CAGATAACAA GCTTGGGGAT 
GAAGGCTGCA GTTTCCTGCT GGAAAACCTC TCCTGGATTT CAATTTCAAA GCAGCTGAAT 
CTGAGTCACA ACCTGCTGTC TGTTACTGGC ATTTACAGTC TGTTGAAGGC AGTGAATACC 
TGTCAGAGGG TGGTGGAGGT GGAAGTCAGC CTTTATCATA ACACTGCAGT CCTGAGGTTT 
ACAGAAGATG GAGAATTTGC TTCCCCTCCT GCTAGTAGGG AAGAGCCTTT GTACCCTACT 
GATGACCAGT GGGATGACAA AGAAAACCAG ACACCTGTTC CCTCCAAAAA AATAAGATTG 
ACATACTGTC GTTTTCAAGC CAGTGACCTG GAGAAGCTGT GTGCAGTCCT GAAGGAATGT 
GGCAGCATCT CTGAGCTGGA CCTCTCAAAT AATTACCTGG GAGATGAAGG ACTTGCACAG 
CTGTTGCAGT TCCTCCCGAA TCTGAAAACG TTGAGGTCAC TCAAGCTAAA TAACAATCAC 
CTCTCCCTGA GCTCTGTGTT CTGCTTAGCT CAGTCCTTGT GTACACTGGA ACACTTGGAA 
ACAATGGACC TCAGCTTAGG ACGCATGCAG GTGGTTCATC TAACCTTTGG GGACAGAATC 
AGGCTCAGAA GGACCAGCAG ATGGAGAAGT TGTTTCCTGG TGCATCCGAA GCACGTAACA 
AATGGACAGT GCTTTCGTCT CAGGAACTGC ACGGTGGGTC CTGAGGACGT GACCAGGCTC 
TGCCAGATGC TGATGCAGTG TACCCAGCTG ACGGAAATCG ATCTGTGTGG AAATGCACTG 
AATGACCAAA GCATTGAGAG ATTACTGAGC TTCCTGCCAC ACTTCTGCCA GCTGACACTA 
CTGAGTATTA GGAACAACAC CTTTTCCCCA TGTTGTGCTG TATTATTTGC CAACTCGATC 
AATCTCTGTG AGCGCATCAG AAGGGTGGAA GTCCGGTCAA GTCAGAATGC TTTTTTACAC 
TTAAGAACAA GCACGCAGGG CCAGAAGACA TCCTGCAGGC TGACTGACTG TGCCATCGGC 
CAAAGGCAGA TAGAGAAGCT CTGCAGAGTC CTGGAGCAGC ATGGGTGTCT GGCAGAACTA 
GATCTCTCCA GGAATCAGCT GGGAGATGAA GTCTTGAGGT TCCTCTTGGA CCACTTGCAT 
CGAGTGCCTG TTACCTGTTC TCTCAATCTC AGCCATAATA GGATTTCTCA GCGTGGAGTT 
CTGCATCTCA TAAATACTTT TACCACTTCT GGAAATACCA CCGAAGTCCA GGTCAGTCTG 
TGCTCCAAGG CAACTTTAAT CATCAAGATG ACAAGCAGAG ATGACCCAAG AAAGATTTTG 
AGGCTCACAG AGTGCAGCTT TCAGCCAGAA CACTTGGAAA AGCTATGTTT GGTTCTGGAG 
AACTGCACCA ATCTGACAGA GTGCATATCC TCCAACAACA ACGTGACAGT CGATGCTGCA 
GGGGGGCTTT TACGTTCACT GAGTAAAACT CCAGGTCCTC TAAAAATCAG CATAGAAGAG 
CCCTGGGTAT GTAAGTTGAG TGTCACGAGC CTTCTTGAGC TGGCTGTCCA GGCCCATGGA 
AACATCACTG CGATCATGAT ATGCAAAGAG AAGAACCTCT TCCAACTAGG TGTAAGGCTT 
CCACACTGCC TGGAGAAGGT GGGATCAGTC GTTAGCAGAT TGAATTTGAA CGAGCCAGAA 
ATCAAACAGG CTTGCTTCTA CCAAAGGGTT CATGACAAAT GCACCCAGCT TCAGGAACTG 
AGATGGTCTC ACGTTGAACT CCACGATGAC ACAGAAATGC TCGTCAGTAT TTTGCTACCT 
CTTCCAGATC TGAAGAAGTT TGAGCTGACC TCTTGCAGCT TTACACCAAC TGGAATTGAT 
TGCTTGATCA CAGGCTTGCA GAGGTGCCAG GCCATTGAAG AGCTCAACCT GGGCCACATG 
AAGCTCGGCG ATGCTGCCAT TCCTAAGCTA GTGTTTGGAC TCTGTGAAAT GCCGTCTCTG 
AAAAGGCTGA TCTTGAACCA TAACAGTATT GGTGACGATG GCTGCTCCAG ACTCGCAGAA 
GCCCTGAGCA GCATGCACTG CATGGAAGAA ATCAATTTAG GCCACAACAA GATTGGAGAT 
CTAGGCCTGA TAAATATAGC TGCTGTCCTG CTGGAAATGC AAAACTTGAA GAGAATCGAC 
CTCTCAGGGA ACTGTCCCAG TCCTGCTGGA GGAGAAAAGC TGATGGAAGC TCTTGCCAAT 
TGCAAGCACC TCGAGGAGCT AATACTGTCA AGGAATGATT TTGGAGATGG GACAGCAGTG 
AAACTTGCCC TCTGTCTGCC TCACATGAAC AGGCTGAAGA TCCTGCACTT GCAGCACAAT 
AACATCGGGC CAGCAGGAGG AACGGAGCTG GCCAGAGCCC TGATGGCATG TGAACTGTTA 
GAAGAGATAA GTTTATCTGA AAACAACCTT GGGGAAGGAG GTATCCGTGC CCTGTCCGAG 
GGGCTGCCGC GTTTTGAGCA CCTCCGGAAG ATTGAGTTGA GACTCTGTGG AATCACTGAT 
GATGCTTCAA AATTACTCTC TCGTGGCTTC CAGCAATGCC CTGCTGTGGA GGAGATCATA 
TTGTCTTGGA ACGCCCTTGG TGATGGAGGA GCCCAAGTGT TGGCCAGCAT CCTCCCAGGG 
ATGGAAACGC TGAAGATGTT AGACCTGGAG AAAAATCTCA TTGGTGCGTG TGGTGCTACC 
AGGTTGGCAG AGGAACTTGT CAGGTGCCCA GAAATTCAGT TCATAAGGCT GTGGGACAAT 
CCGGTGCCCA AAGGCCTCGC TGAAAGTTTG ACAAGCCAAG ACCCAAGACT GTGCTTCTCC 
TTCTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRC5 ( XM_015292328.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147814.1 Gallus gallus NLR family, CARD domain containing 5 (NLRC5), transcript variant X4, mRNA.

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CloneID	OGa13511
Clone ID Related Accession (Same CDS sequence)	XM_004944054.2 , XM_015292328.1 , XM_015292327.1
Accession Version	XM_015292328.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	protein NLRC5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455926.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCA CACTCCAGAA CGATGACTTC AACCTGGAAG GAGCTGTAGC AAGCATCCGC 
CCCCAGCTAG TGGAGTTCCT CAGCCATCGT CTCGACTGGC TGCTCTCTGC TTCCCAGCAC 
TTCCTCCCTA CGGCTGTCCT CAGCGGCTTG GCAGGCGTCA CTGACCACAG AGAGAAAGTC 
TCTGTGCTCC TCGACCTGCT GGAGAAGGCT GGCCATGCCA CCTGGAAACA GTTTGCACAG 
TGCCTCTGCA TGGAGTGTGA CCTGCCCCTG GAAATGGAGA TACTGCTCAT GAGTTCTGCA 
GGAGAAGGTA ACTTGACTCA GAAGCAAGAA GCTCAGACAG ACGCAAGTGA CCAGTCCTCT 
GTGCCAAGAG GGTTGACGCG CCGCCGCCGT AGCAGCAGCC CCATCAGTGA TAAGGGTGCT 
AAACAGAAAC GCCTGGACTC AGCTGAAAAG TATCAACGTC TTCTCGTTGA CTCCATCTGC 
AAGAGATATG GAAGCAGGAG AGCAGCAGGA GCAGCGGCAC AAGAGCCAAC GCAGCCTCTG 
GCTTTCAGCC AAGCCTTTGT CAACCTCGTT CTTCGGCAGA GCAAGGCCTC CCGACTGAAA 
GAGAGAACAG ACAAACCCCG GGAGGACGTG CCCAGTGCCC CCGAGCCAGA AGAATGTGTG 
GATACAGCCG TGAGAGTGTC AGATCTCTTT GGCAGCGTGG TCCGATCGGG TACAACGAAG 
GTGATCTTTT TGTTTGGTAA ACCAGGTACA GGCAAGACCA TGCTGATGCA CAGGATTTGT 
CAGAAGTGGG CGGAAGGTGT CTTACATCAG TTTCTCTTCA CTTTCCTGTT TGAGTTCCGG 
CAGTTGAATT TACTAAAAAG AAAACTGACT CTGAAGGAGC TTTTGTTTGA CCTGTTTCTT 
CAGCCAGAAG ACAGCCCCGA TGCAGTGTTC CAGCACCTCC TGGAAAACGC CCAACGTACA 
CTGATCATCT TTGATGGGCT GGATGAGTTT GTGGGTAACA TGGACGTGTC ATCAGCTTCT 
AAAGGAAGCC TGAACCGTAG CTCCAGTATG TCCATATCTG AACTCTTTGC AGAGCTTTGT 
CATGGGAACC TCCTACCTGG CTGCACGGTG TTGGTCACCA GCCGCCCTAA GAGAGTCCCT 
GACTTCTTGA TAAACACAGT AGATTTGCTG GCAGAAGTTT GGGGGTTTGA TCACGAGAAG 
GTTGAGGAGT ATGTCAGCCA CTATTTTCGT CATCATTCAT TCAAAGAACA AGCCATCGCT 
CACCTGAAGA ACAACACCAA GCTCCTCAGC ATGTGCCAGA TCCCTGCCCT GTGTTACGTT 
GTCTGCATCT GTTTGGAGTA TTTGCTCCTT AAGCATCAGA CGAGCGTGGA GCTTCCCCAG 
ACCATGACAC AGTTTTATAT CAAAATGCTT CTCATCTTCA TAAACAAACA GCAGGGGGAG 
CATGCAGGTG ATGAGGAGGC TCAGCTGAAC AGTAACAAGA AGGCCATTTT GGGTCTGTGT 
GATCTTGCAC TGAAAGGCCT GGAAGCTAAG AAGCTCGTGT TTTACGTTGG AGATATTCCA 
GAGCACGTGA AAGAGTTTGC TTCCTTGCAC GGTCTGCTGA CTGTCTTTGA GGTGAAGACA 
AACAGCGCTC ACCCCGAGAC TGGCTATGCC TTTGTGCACT TGAGCCTGCA GGAGTTTTTT 
GCAGCCCTGT GTCTCATGAT AAGCAAGAGT GTGGACAAGA GCCACCTGAA GAGGAAGTTA 
TCTCTGAAGT CAAAGTGGAC GTTAAGGAAT GAAGCAAAGA CTGGGTTCAT GGAGAGCTTT 
CACATCTTCC TCTCAGGCTT GTCATCCAAG GAGTGTAGGA CGTTTATCAT GTTGCTAGCT 
GAACAAAGTG AAGCTTGGGT GCAGGATAAG CAAGATGCAA TCCTTCAGTC CCTCAAGAAA 
CTGGCAGCCA CTCAGCTGAC CGGGCCCAAA GTCATTGAGC TCTGCCATTG CACGTTTGAA 
ACGCAAGGCC TCGAAGTAGC CCAGCACATT GGGAGCCTGC TGAATTTCAA GTACGAGTTT 
AAAAACTTCA GACTGACACC TCTGGACATG TCGGCTTTGG TTTTTGTCAT CAACTCGGGC 
CAGGATGTGA CTCACTTGGA TTTTGCAGGG TGTCCCTTAG ACACGGACTG TTTGGAGATG 
CTGGCCAGCT GTAGGAATGT GGAGCATCTG AGCTTTCGGA GTAGGAGATT TGGTGATGAC 
TTTGCTGCTG CGCTTTCAAA GGGCTTAGGA GAAATGGGGA GCCTGAAGAA ACTAGAGGTG 
ACAGGTGGAA GCATCACAGC TGCGGGGCTG ACTGACATGG TCCAAGCTTT GTCACACTGC 
CTGCAGCTCG AGGAAATAAA CTTGCAGGAC AATCGGATAC AGAATCCAGA CGTAAAGACA 
GTGATGGAGC TGTTTTCCAG AATGGAAAAG CTGAAGAAAA TAGATCTGAG CAAGAACAGT 
CTTTCCTTGA ATGCTGTTCT CCTTTTGGCA AAGGAGTTCA TCGCGTGTCA GAATGCTGCT 
GAGCTACACG TCAGGAAGGA CACTGTGATA ATATCTTTCT CGGGCCCATC TGGGAAAGTT 
CCAAGGTCTT TGGATTTGAA GCGGGAACAA AACAAGGAAT GTGTGACTCC AACTAGACAT 
CTGAGGTTGT GCTTGCAGGC TCGCTGCTTG TCTTCACAAC ATGCAAAGGA GATTGTGTCC 
ATTCTGCAGA GCTGCCCACA CCTCTCTGAA GTAGATTTAT CAGATAACAA GCTTGGGGAT 
GAAGGCTGCA GTTTCCTGCT GGAAAACCTC TCCTGGATTT CAATTTCAAA GCAGCTGAAT 
CTGAGTCACA ACCTGCTGTC TGTTACTGGC ATTTACAGTC TGTTGAAGGC AGTGAATACC 
TGTCAGAGGG TGGTGGAGGT GGAAGTCAGC CTTTATCATA ACACTGCAGT CCTGAGGTTT 
ACAGAAGATG GAGAATTTGC TTCCCCTCCT GCTAGTAGGG AAGAGCCTTT GTACCCTACT 
GATGACCAGT GGGATGACAA AGAAAACCAG ACACCTGTTC CCTCCAAAAA AATAAGATTG 
ACATACTGTC GTTTTCAAGC CAGTGACCTG GAGAAGCTGT GTGCAGTCCT GAAGGAATGT 
GGCAGCATCT CTGAGCTGGA CCTCTCAAAT AATTACCTGG GAGATGAAGG ACTTGCACAG 
CTGTTGCAGT TCCTCCCGAA TCTGAAAACG TTGAGGTCAC TCAAGCTAAA TAACAATCAC 
CTCTCCCTGA GCTCTGTGTT CTGCTTAGCT CAGTCCTTGT GTACACTGGA ACACTTGGAA 
ACAATGGACC TCAGCTTAGG ACGCATGCAG GTGGTTCATC TAACCTTTGG GGACAGAATC 
AGGCTCAGAA GGACCAGCAG ATGGAGAAGT TGTTTCCTGG TGCATCCGAA GCACGTAACA 
AATGGACAGT GCTTTCGTCT CAGGAACTGC ACGGTGGGTC CTGAGGACGT GACCAGGCTC 
TGCCAGATGC TGATGCAGTG TACCCAGCTG ACGGAAATCG ATCTGTGTGG AAATGCACTG 
AATGACCAAA GCATTGAGAG ATTACTGAGC TTCCTGCCAC ACTTCTGCCA GCTGACACTA 
CTGAGTATTA GGAACAACAC CTTTTCCCCA TGTTGTGCTG TATTATTTGC CAACTCGATC 
AATCTCTGTG AGCGCATCAG AAGGGTGGAA GTCCGGTCAA GTCAGAATGC TTTTTTACAC 
TTAAGAACAA GCACGCAGGG CCAGAAGACA TCCTGCAGGC TGACTGACTG TGCCATCGGC 
CAAAGGCAGA TAGAGAAGCT CTGCAGAGTC CTGGAGCAGC ATGGGTGTCT GGCAGAACTA 
GATCTCTCCA GGAATCAGCT GGGAGATGAA GTCTTGAGGT TCCTCTTGGA CCACTTGCAT 
CGAGTGCCTG TTACCTGTTC TCTCAATCTC AGCCATAATA GGATTTCTCA GCGTGGAGTT 
CTGCATCTCA TAAATACTTT TACCACTTCT GGAAATACCA CCGAAGTCCA GGTCAGTCTG 
TGCTCCAAGG CAACTTTAAT CATCAAGATG ACAAGCAGAG ATGACCCAAG AAAGATTTTG 
AGGCTCACAG AGTGCAGCTT TCAGCCAGAA CACTTGGAAA AGCTATGTTT GGTTCTGGAG 
AACTGCACCA ATCTGACAGA GTGCATATCC TCCAACAACA ACGTGACAGT CGATGCTGCA 
GGGGGGCTTT TACGTTCACT GAGTAAAACT CCAGGTCCTC TAAAAATCAG CATAGAAGAG 
CCCTGGGTAT GTAAGTTGAG TGTCACGAGC CTTCTTGAGC TGGCTGTCCA GGCCCATGGA 
AACATCACTG CGATCATGAT ATGCAAAGAG AAGAACCTCT TCCAACTAGG TGTAAGGCTT 
CCACACTGCC TGGAGAAGGT GGGATCAGTC GTTAGCAGAT TGAATTTGAA CGAGCCAGAA 
ATCAAACAGG CTTGCTTCTA CCAAAGGGTT CATGACAAAT GCACCCAGCT TCAGGAACTG 
AGATGGTCTC ACGTTGAACT CCACGATGAC ACAGAAATGC TCGTCAGTAT TTTGCTACCT 
CTTCCAGATC TGAAGAAGTT TGAGCTGACC TCTTGCAGCT TTACACCAAC TGGAATTGAT 
TGCTTGATCA CAGGCTTGCA GAGGTGCCAG GCCATTGAAG AGCTCAACCT GGGCCACATG 
AAGCTCGGCG ATGCTGCCAT TCCTAAGCTA GTGTTTGGAC TCTGTGAAAT GCCGTCTCTG 
AAAAGGCTGA TCTTGAACCA TAACAGTATT GGTGACGATG GCTGCTCCAG ACTCGCAGAA 
GCCCTGAGCA GCATGCACTG CATGGAAGAA ATCAATTTAG GCCACAACAA GATTGGAGAT 
CTAGGCCTGA TAAATATAGC TGCTGTCCTG CTGGAAATGC AAAACTTGAA GAGAATCGAC 
CTCTCAGGGA ACTGTCCCAG TCCTGCTGGA GGAGAAAAGC TGATGGAAGC TCTTGCCAAT 
TGCAAGCACC TCGAGGAGCT AATACTGTCA AGGAATGATT TTGGAGATGG GACAGCAGTG 
AAACTTGCCC TCTGTCTGCC TCACATGAAC AGGCTGAAGA TCCTGCACTT GCAGCACAAT 
AACATCGGGC CAGCAGGAGG AACGGAGCTG GCCAGAGCCC TGATGGCATG TGAACTGTTA 
GAAGAGATAA GTTTATCTGA AAACAACCTT GGGGAAGGAG GTATCCGTGC CCTGTCCGAG 
GGGCTGCCGC GTTTTGAGCA CCTCCGGAAG ATTGAGTTGA GACTCTGTGG AATCACTGAT 
GATGCTTCAA AATTACTCTC TCGTGGCTTC CAGCAATGCC CTGCTGTGGA GGAGATCATA 
TTGTCTTGGA ACGCCCTTGG TGATGGAGGA GCCCAAGTGT TGGCCAGCAT CCTCCCAGGG 
ATGGAAACGC TGAAGATGTT AGACCTGGAG AAAAATCTCA TTGGTGCGTG TGGTGCTACC 
AGGTTGGCAG AGGAACTTGT CAGGTGCCCA GAAATTCAGT TCATAAGGCT GTGGGACAAT 
CCGGTGCCCA AAGGCCTCGC TGAAAGTTTG ACAAGCCAAG ACCCAAGACT GTGCTTCTCC 
TTCTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147814.1
CDS	282..5870
Translation	MQPTLQNDDFNLEGAVASIRPQLVEFLSHRLDWLLSASQHFLPTAVLSGLAGVTDHREKVSVLLDLLEKAGHATWKQFAQCLCMECDLPLEMEILLMSSAGEGNLTQKQEAQTDASDQSSVPRGLTRRRRSSSPISDKGAKQKRLDSAEKYQRLLVDSICKRYGSRRAAGAAAQEPTQPLAFSQAFVNLVLRQSKASRLKERTDKPREDVPSAPEPEECVDTAVRVSDLFGSVVRSGTTKVIFLFGKPGTGKTMLMHRICQKWAEGVLHQFLFTFLFEFRQLNLLKRKLTLKELLFDLFLQPEDSPDAVFQHLLENAQRTLIIFDGLDEFVGNMDVSSASKGSLNRSSSMSISELFAELCHGNLLPGCTVLVTSRPKRVPDFLINTVDLLAEVWGFDHEKVEEYVSHYFRHHSFKEQAIAHLKNNTKLLSMCQIPALCYVVCICLEYLLLKHQTSVELPQTMTQFYIKMLLIFINKQQGEHAGDEEAQLNSNKKAILGLCDLALKGLEAKKLVFYVGDIPEHVKEFASLHGLLTVFEVKTNSAHPETGYAFVHLSLQEFFAALCLMISKSVDKSHLKRKLSLKSKWTLRNEAKTGFMESFHIFLSGLSSKECRTFIMLLAEQSEAWVQDKQDAILQSLKKLAATQLTGPKVIELCHCTFETQGLEVAQHIGSLLNFKYEFKNFRLTPLDMSALVFVINSGQDVTHLDFAGCPLDTDCLEMLASCRNVEHLSFRSRRFGDDFAAALSKGLGEMGSLKKLEVTGGSITAAGLTDMVQALSHCLQLEEINLQDNRIQNPDVKTVMELFSRMEKLKKIDLSKNSLSLNAVLLLAKEFIACQNAAELHVRKDTVIISFSGPSGKVPRSLDLKREQNKECVTPTRHLRLCLQARCLSSQHAKEIVSILQSCPHLSEVDLSDNKLGDEGCSFLLENLSWISISKQLNLSHNLLSVTGIYSLLKAVNTCQRVVEVEVSLYHNTAVLRFTEDGEFASPPASREEPLYPTDDQWDDKENQTPVPSKKIRLTYCRFQASDLEKLCAVLKECGSISELDLSNNYLGDEGLAQLLQFLPNLKTLRSLKLNNNHLSLSSVFCLAQSLCTLEHLETMDLSLGRMQVVHLTFGDRIRLRRTSRWRSCFLVHPKHVTNGQCFRLRNCTVGPEDVTRLCQMLMQCTQLTEIDLCGNALNDQSIERLLSFLPHFCQLTLLSIRNNTFSPCCAVLFANSINLCERIRRVEVRSSQNAFLHLRTSTQGQKTSCRLTDCAIGQRQIEKLCRVLEQHGCLAELDLSRNQLGDEVLRFLLDHLHRVPVTCSLNLSHNRISQRGVLHLINTFTTSGNTTEVQVSLCSKATLIIKMTSRDDPRKILRLTECSFQPEHLEKLCLVLENCTNLTECISSNNNVTVDAAGGLLRSLSKTPGPLKISIEEPWVCKLSVTSLLELAVQAHGNITAIMICKEKNLFQLGVRLPHCLEKVGSVVSRLNLNEPEIKQACFYQRVHDKCTQLQELRWSHVELHDDTEMLVSILLPLPDLKKFELTSCSFTPTGIDCLITGLQRCQAIEELNLGHMKLGDAAIPKLVFGLCEMPSLKRLILNHNSIGDDGCSRLAEALSSMHCMEEINLGHNKIGDLGLINIAAVLLEMQNLKRIDLSGNCPSPAGGEKLMEALANCKHLEELILSRNDFGDGTAVKLALCLPHMNRLKILHLQHNNIGPAGGTELARALMACELLEEISLSENNLGEGGIRALSEGLPRFEHLRKIELRLCGITDDASKLLSRGFQQCPAVEEIILSWNALGDGGAQVLASILPGMETLKMLDLEKNLIGACGATRLAEELVRCPEIQFIRLWDNPVPKGLAESLTSQDPRLCFSFC

Target ORF information:

RefSeq Version	XM_015292328.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NLR family, CARD domain containing 5 (NLRC5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292328.1

ORF Insert Sequence:

ATGCAGCCCA CACTCCAGAA CGATGACTTC AACCTGGAAG GAGCTGTAGC AAGCATCCGC 
CCCCAGCTAG TGGAGTTCCT CAGCCATCGT CTCGACTGGC TGCTCTCTGC TTCCCAGCAC 
TTCCTCCCTA CGGCTGTCCT CAGCGGCTTG GCAGGCGTCA CTGACCACAG AGAGAAAGTC 
TCTGTGCTCC TCGACCTGCT GGAGAAGGCT GGCCATGCCA CCTGGAAACA GTTTGCACAG 
TGCCTCTGCA TGGAGTGTGA CCTGCCCCTG GAAATGGAGA TACTGCTCAT GAGTTCTGCA 
GGAGAAGGTA ACTTGACTCA GAAGCAAGAA GCTCAGACAG ACGCAAGTGA CCAGTCCTCT 
GTGCCAAGAG GGTTGACGCG CCGCCGCCGT AGCAGCAGCC CCATCAGTGA TAAGGGTGCT 
AAACAGAAAC GCCTGGACTC AGCTGAAAAG TATCAACGTC TTCTCGTTGA CTCCATCTGC 
AAGAGATATG GAAGCAGGAG AGCAGCAGGA GCAGCGGCAC AAGAGCCAAC GCAGCCTCTG 
GCTTTCAGCC AAGCCTTTGT CAACCTCGTT CTTCGGCAGA GCAAGGCCTC CCGACTGAAA 
GAGAGAACAG ACAAACCCCG GGAGGACGTG CCCAGTGCCC CCGAGCCAGA AGAATGTGTG 
GATACAGCCG TGAGAGTGTC AGATCTCTTT GGCAGCGTGG TCCGATCGGG TACAACGAAG 
GTGATCTTTT TGTTTGGTAA ACCAGGTACA GGCAAGACCA TGCTGATGCA CAGGATTTGT 
CAGAAGTGGG CGGAAGGTGT CTTACATCAG TTTCTCTTCA CTTTCCTGTT TGAGTTCCGG 
CAGTTGAATT TACTAAAAAG AAAACTGACT CTGAAGGAGC TTTTGTTTGA CCTGTTTCTT 
CAGCCAGAAG ACAGCCCCGA TGCAGTGTTC CAGCACCTCC TGGAAAACGC CCAACGTACA 
CTGATCATCT TTGATGGGCT GGATGAGTTT GTGGGTAACA TGGACGTGTC ATCAGCTTCT 
AAAGGAAGCC TGAACCGTAG CTCCAGTATG TCCATATCTG AACTCTTTGC AGAGCTTTGT 
CATGGGAACC TCCTACCTGG CTGCACGGTG TTGGTCACCA GCCGCCCTAA GAGAGTCCCT 
GACTTCTTGA TAAACACAGT AGATTTGCTG GCAGAAGTTT GGGGGTTTGA TCACGAGAAG 
GTTGAGGAGT ATGTCAGCCA CTATTTTCGT CATCATTCAT TCAAAGAACA AGCCATCGCT 
CACCTGAAGA ACAACACCAA GCTCCTCAGC ATGTGCCAGA TCCCTGCCCT GTGTTACGTT 
GTCTGCATCT GTTTGGAGTA TTTGCTCCTT AAGCATCAGA CGAGCGTGGA GCTTCCCCAG 
ACCATGACAC AGTTTTATAT CAAAATGCTT CTCATCTTCA TAAACAAACA GCAGGGGGAG 
CATGCAGGTG ATGAGGAGGC TCAGCTGAAC AGTAACAAGA AGGCCATTTT GGGTCTGTGT 
GATCTTGCAC TGAAAGGCCT GGAAGCTAAG AAGCTCGTGT TTTACGTTGG AGATATTCCA 
GAGCACGTGA AAGAGTTTGC TTCCTTGCAC GGTCTGCTGA CTGTCTTTGA GGTGAAGACA 
AACAGCGCTC ACCCCGAGAC TGGCTATGCC TTTGTGCACT TGAGCCTGCA GGAGTTTTTT 
GCAGCCCTGT GTCTCATGAT AAGCAAGAGT GTGGACAAGA GCCACCTGAA GAGGAAGTTA 
TCTCTGAAGT CAAAGTGGAC GTTAAGGAAT GAAGCAAAGA CTGGGTTCAT GGAGAGCTTT 
CACATCTTCC TCTCAGGCTT GTCATCCAAG GAGTGTAGGA CGTTTATCAT GTTGCTAGCT 
GAACAAAGTG AAGCTTGGGT GCAGGATAAG CAAGATGCAA TCCTTCAGTC CCTCAAGAAA 
CTGGCAGCCA CTCAGCTGAC CGGGCCCAAA GTCATTGAGC TCTGCCATTG CACGTTTGAA 
ACGCAAGGCC TCGAAGTAGC CCAGCACATT GGGAGCCTGC TGAATTTCAA GTACGAGTTT 
AAAAACTTCA GACTGACACC TCTGGACATG TCGGCTTTGG TTTTTGTCAT CAACTCGGGC 
CAGGATGTGA CTCACTTGGA TTTTGCAGGG TGTCCCTTAG ACACGGACTG TTTGGAGATG 
CTGGCCAGCT GTAGGAATGT GGAGCATCTG AGCTTTCGGA GTAGGAGATT TGGTGATGAC 
TTTGCTGCTG CGCTTTCAAA GGGCTTAGGA GAAATGGGGA GCCTGAAGAA ACTAGAGGTG 
ACAGGTGGAA GCATCACAGC TGCGGGGCTG ACTGACATGG TCCAAGCTTT GTCACACTGC 
CTGCAGCTCG AGGAAATAAA CTTGCAGGAC AATCGGATAC AGAATCCAGA CGTAAAGACA 
GTGATGGAGC TGTTTTCCAG AATGGAAAAG CTGAAGAAAA TAGATCTGAG CAAGAACAGT 
CTTTCCTTGA ATGCTGTTCT CCTTTTGGCA AAGGAGTTCA TCGCGTGTCA GAATGCTGCT 
GAGCTACACG TCAGGAAGGA CACTGTGATA ATATCTTTCT CGGGCCCATC TGGGAAAGTT 
CCAAGGTCTT TGGATTTGAA GCGGGAACAA AACAAGGAAT GTGTGACTCC AACTAGACAT 
CTGAGGTTGT GCTTGCAGGC TCGCTGCTTG TCTTCACAAC ATGCAAAGGA GATTGTGTCC 
ATTCTGCAGA GCTGCCCACA CCTCTCTGAA GTAGATTTAT CAGATAACAA GCTTGGGGAT 
GAAGGCTGCA GTTTCCTGCT GGAAAACCTC TCCTGGATTT CAATTTCAAA GCAGCTGAAT 
CTGAGTCACA ACCTGCTGTC TGTTACTGGC ATTTACAGTC TGTTGAAGGC AGTGAATACC 
TGTCAGAGGG TGGTGGAGGT GGAAGTCAGC CTTTATCATA ACACTGCAGT CCTGAGGTTT 
ACAGAAGATG GAGAATTTGC TTCCCCTCCT GCTAGTAGGG AAGAGCCTTT GTACCCTACT 
GATGACCAGT GGGATGACAA AGAAAACCAG ACACCTGTTC CCTCCAAAAA AATAAGATTG 
ACATACTGTC GTTTTCAAGC CAGTGACCTG GAGAAGCTGT GTGCAGTCCT GAAGGAATGT 
GGCAGCATCT CTGAGCTGGA CCTCTCAAAT AATTACCTGG GAGATGAAGG ACTTGCACAG 
CTGTTGCAGT TCCTCCCGAA TCTGAAAACG TTGAGGTCAC TCAAGCTAAA TAACAATCAC 
CTCTCCCTGA GCTCTGTGTT CTGCTTAGCT CAGTCCTTGT GTACACTGGA ACACTTGGAA 
ACAATGGACC TCAGCTTAGG ACGCATGCAG GTGGTTCATC TAACCTTTGG GGACAGAATC 
AGGCTCAGAA GGACCAGCAG ATGGAGAAGT TGTTTCCTGG TGCATCCGAA GCACGTAACA 
AATGGACAGT GCTTTCGTCT CAGGAACTGC ACGGTGGGTC CTGAGGACGT GACCAGGCTC 
TGCCAGATGC TGATGCAGTG TACCCAGCTG ACGGAAATCG ATCTGTGTGG AAATGCACTG 
AATGACCAAA GCATTGAGAG ATTACTGAGC TTCCTGCCAC ACTTCTGCCA GCTGACACTA 
CTGAGTATTA GGAACAACAC CTTTTCCCCA TGTTGTGCTG TATTATTTGC CAACTCGATC 
AATCTCTGTG AGCGCATCAG AAGGGTGGAA GTCCGGTCAA GTCAGAATGC TTTTTTACAC 
TTAAGAACAA GCACGCAGGG CCAGAAGACA TCCTGCAGGC TGACTGACTG TGCCATCGGC 
CAAAGGCAGA TAGAGAAGCT CTGCAGAGTC CTGGAGCAGC ATGGGTGTCT GGCAGAACTA 
GATCTCTCCA GGAATCAGCT GGGAGATGAA GTCTTGAGGT TCCTCTTGGA CCACTTGCAT 
CGAGTGCCTG TTACCTGTTC TCTCAATCTC AGCCATAATA GGATTTCTCA GCGTGGAGTT 
CTGCATCTCA TAAATACTTT TACCACTTCT GGAAATACCA CCGAAGTCCA GGTCAGTCTG 
TGCTCCAAGG CAACTTTAAT CATCAAGATG ACAAGCAGAG ATGACCCAAG AAAGATTTTG 
AGGCTCACAG AGTGCAGCTT TCAGCCAGAA CACTTGGAAA AGCTATGTTT GGTTCTGGAG 
AACTGCACCA ATCTGACAGA GTGCATATCC TCCAACAACA ACGTGACAGT CGATGCTGCA 
GGGGGGCTTT TACGTTCACT GAGTAAAACT CCAGGTCCTC TAAAAATCAG CATAGAAGAG 
CCCTGGGTAT GTAAGTTGAG TGTCACGAGC CTTCTTGAGC TGGCTGTCCA GGCCCATGGA 
AACATCACTG CGATCATGAT ATGCAAAGAG AAGAACCTCT TCCAACTAGG TGTAAGGCTT 
CCACACTGCC TGGAGAAGGT GGGATCAGTC GTTAGCAGAT TGAATTTGAA CGAGCCAGAA 
ATCAAACAGG CTTGCTTCTA CCAAAGGGTT CATGACAAAT GCACCCAGCT TCAGGAACTG 
AGATGGTCTC ACGTTGAACT CCACGATGAC ACAGAAATGC TCGTCAGTAT TTTGCTACCT 
CTTCCAGATC TGAAGAAGTT TGAGCTGACC TCTTGCAGCT TTACACCAAC TGGAATTGAT 
TGCTTGATCA CAGGCTTGCA GAGGTGCCAG GCCATTGAAG AGCTCAACCT GGGCCACATG 
AAGCTCGGCG ATGCTGCCAT TCCTAAGCTA GTGTTTGGAC TCTGTGAAAT GCCGTCTCTG 
AAAAGGCTGA TCTTGAACCA TAACAGTATT GGTGACGATG GCTGCTCCAG ACTCGCAGAA 
GCCCTGAGCA GCATGCACTG CATGGAAGAA ATCAATTTAG GCCACAACAA GATTGGAGAT 
CTAGGCCTGA TAAATATAGC TGCTGTCCTG CTGGAAATGC AAAACTTGAA GAGAATCGAC 
CTCTCAGGGA ACTGTCCCAG TCCTGCTGGA GGAGAAAAGC TGATGGAAGC TCTTGCCAAT 
TGCAAGCACC TCGAGGAGCT AATACTGTCA AGGAATGATT TTGGAGATGG GACAGCAGTG 
AAACTTGCCC TCTGTCTGCC TCACATGAAC AGGCTGAAGA TCCTGCACTT GCAGCACAAT 
AACATCGGGC CAGCAGGAGG AACGGAGCTG GCCAGAGCCC TGATGGCATG TGAACTGTTA 
GAAGAGATAA GTTTATCTGA AAACAACCTT GGGGAAGGAG GTATCCGTGC CCTGTCCGAG 
GGGCTGCCGC GTTTTGAGCA CCTCCGGAAG ATTGAGTTGA GACTCTGTGG AATCACTGAT 
GATGCTTCAA AATTACTCTC TCGTGGCTTC CAGCAATGCC CTGCTGTGGA GGAGATCATA 
TTGTCTTGGA ACGCCCTTGG TGATGGAGGA GCCCAAGTGT TGGCCAGCAT CCTCCCAGGG 
ATGGAAACGC TGAAGATGTT AGACCTGGAG AAAAATCTCA TTGGTGCGTG TGGTGCTACC 
AGGTTGGCAG AGGAACTTGT CAGGTGCCCA GAAATTCAGT TCATAAGGCT GTGGGACAAT 
CCGGTGCCCA AAGGCCTCGC TGAAAGTTTG ACAAGCCAAG ACCCAAGACT GTGCTTCTCC 
TTCTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRC5 ( XM_015292327.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147813.1 Gallus gallus NLR family, CARD domain containing 5 (NLRC5), transcript variant X3, mRNA.

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CloneID	OGa13511
Clone ID Related Accession (Same CDS sequence)	XM_004944054.2 , XM_015292328.1 , XM_015292327.1
Accession Version	XM_015292327.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	protein NLRC5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455926.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCA CACTCCAGAA CGATGACTTC AACCTGGAAG GAGCTGTAGC AAGCATCCGC 
CCCCAGCTAG TGGAGTTCCT CAGCCATCGT CTCGACTGGC TGCTCTCTGC TTCCCAGCAC 
TTCCTCCCTA CGGCTGTCCT CAGCGGCTTG GCAGGCGTCA CTGACCACAG AGAGAAAGTC 
TCTGTGCTCC TCGACCTGCT GGAGAAGGCT GGCCATGCCA CCTGGAAACA GTTTGCACAG 
TGCCTCTGCA TGGAGTGTGA CCTGCCCCTG GAAATGGAGA TACTGCTCAT GAGTTCTGCA 
GGAGAAGGTA ACTTGACTCA GAAGCAAGAA GCTCAGACAG ACGCAAGTGA CCAGTCCTCT 
GTGCCAAGAG GGTTGACGCG CCGCCGCCGT AGCAGCAGCC CCATCAGTGA TAAGGGTGCT 
AAACAGAAAC GCCTGGACTC AGCTGAAAAG TATCAACGTC TTCTCGTTGA CTCCATCTGC 
AAGAGATATG GAAGCAGGAG AGCAGCAGGA GCAGCGGCAC AAGAGCCAAC GCAGCCTCTG 
GCTTTCAGCC AAGCCTTTGT CAACCTCGTT CTTCGGCAGA GCAAGGCCTC CCGACTGAAA 
GAGAGAACAG ACAAACCCCG GGAGGACGTG CCCAGTGCCC CCGAGCCAGA AGAATGTGTG 
GATACAGCCG TGAGAGTGTC AGATCTCTTT GGCAGCGTGG TCCGATCGGG TACAACGAAG 
GTGATCTTTT TGTTTGGTAA ACCAGGTACA GGCAAGACCA TGCTGATGCA CAGGATTTGT 
CAGAAGTGGG CGGAAGGTGT CTTACATCAG TTTCTCTTCA CTTTCCTGTT TGAGTTCCGG 
CAGTTGAATT TACTAAAAAG AAAACTGACT CTGAAGGAGC TTTTGTTTGA CCTGTTTCTT 
CAGCCAGAAG ACAGCCCCGA TGCAGTGTTC CAGCACCTCC TGGAAAACGC CCAACGTACA 
CTGATCATCT TTGATGGGCT GGATGAGTTT GTGGGTAACA TGGACGTGTC ATCAGCTTCT 
AAAGGAAGCC TGAACCGTAG CTCCAGTATG TCCATATCTG AACTCTTTGC AGAGCTTTGT 
CATGGGAACC TCCTACCTGG CTGCACGGTG TTGGTCACCA GCCGCCCTAA GAGAGTCCCT 
GACTTCTTGA TAAACACAGT AGATTTGCTG GCAGAAGTTT GGGGGTTTGA TCACGAGAAG 
GTTGAGGAGT ATGTCAGCCA CTATTTTCGT CATCATTCAT TCAAAGAACA AGCCATCGCT 
CACCTGAAGA ACAACACCAA GCTCCTCAGC ATGTGCCAGA TCCCTGCCCT GTGTTACGTT 
GTCTGCATCT GTTTGGAGTA TTTGCTCCTT AAGCATCAGA CGAGCGTGGA GCTTCCCCAG 
ACCATGACAC AGTTTTATAT CAAAATGCTT CTCATCTTCA TAAACAAACA GCAGGGGGAG 
CATGCAGGTG ATGAGGAGGC TCAGCTGAAC AGTAACAAGA AGGCCATTTT GGGTCTGTGT 
GATCTTGCAC TGAAAGGCCT GGAAGCTAAG AAGCTCGTGT TTTACGTTGG AGATATTCCA 
GAGCACGTGA AAGAGTTTGC TTCCTTGCAC GGTCTGCTGA CTGTCTTTGA GGTGAAGACA 
AACAGCGCTC ACCCCGAGAC TGGCTATGCC TTTGTGCACT TGAGCCTGCA GGAGTTTTTT 
GCAGCCCTGT GTCTCATGAT AAGCAAGAGT GTGGACAAGA GCCACCTGAA GAGGAAGTTA 
TCTCTGAAGT CAAAGTGGAC GTTAAGGAAT GAAGCAAAGA CTGGGTTCAT GGAGAGCTTT 
CACATCTTCC TCTCAGGCTT GTCATCCAAG GAGTGTAGGA CGTTTATCAT GTTGCTAGCT 
GAACAAAGTG AAGCTTGGGT GCAGGATAAG CAAGATGCAA TCCTTCAGTC CCTCAAGAAA 
CTGGCAGCCA CTCAGCTGAC CGGGCCCAAA GTCATTGAGC TCTGCCATTG CACGTTTGAA 
ACGCAAGGCC TCGAAGTAGC CCAGCACATT GGGAGCCTGC TGAATTTCAA GTACGAGTTT 
AAAAACTTCA GACTGACACC TCTGGACATG TCGGCTTTGG TTTTTGTCAT CAACTCGGGC 
CAGGATGTGA CTCACTTGGA TTTTGCAGGG TGTCCCTTAG ACACGGACTG TTTGGAGATG 
CTGGCCAGCT GTAGGAATGT GGAGCATCTG AGCTTTCGGA GTAGGAGATT TGGTGATGAC 
TTTGCTGCTG CGCTTTCAAA GGGCTTAGGA GAAATGGGGA GCCTGAAGAA ACTAGAGGTG 
ACAGGTGGAA GCATCACAGC TGCGGGGCTG ACTGACATGG TCCAAGCTTT GTCACACTGC 
CTGCAGCTCG AGGAAATAAA CTTGCAGGAC AATCGGATAC AGAATCCAGA CGTAAAGACA 
GTGATGGAGC TGTTTTCCAG AATGGAAAAG CTGAAGAAAA TAGATCTGAG CAAGAACAGT 
CTTTCCTTGA ATGCTGTTCT CCTTTTGGCA AAGGAGTTCA TCGCGTGTCA GAATGCTGCT 
GAGCTACACG TCAGGAAGGA CACTGTGATA ATATCTTTCT CGGGCCCATC TGGGAAAGTT 
CCAAGGTCTT TGGATTTGAA GCGGGAACAA AACAAGGAAT GTGTGACTCC AACTAGACAT 
CTGAGGTTGT GCTTGCAGGC TCGCTGCTTG TCTTCACAAC ATGCAAAGGA GATTGTGTCC 
ATTCTGCAGA GCTGCCCACA CCTCTCTGAA GTAGATTTAT CAGATAACAA GCTTGGGGAT 
GAAGGCTGCA GTTTCCTGCT GGAAAACCTC TCCTGGATTT CAATTTCAAA GCAGCTGAAT 
CTGAGTCACA ACCTGCTGTC TGTTACTGGC ATTTACAGTC TGTTGAAGGC AGTGAATACC 
TGTCAGAGGG TGGTGGAGGT GGAAGTCAGC CTTTATCATA ACACTGCAGT CCTGAGGTTT 
ACAGAAGATG GAGAATTTGC TTCCCCTCCT GCTAGTAGGG AAGAGCCTTT GTACCCTACT 
GATGACCAGT GGGATGACAA AGAAAACCAG ACACCTGTTC CCTCCAAAAA AATAAGATTG 
ACATACTGTC GTTTTCAAGC CAGTGACCTG GAGAAGCTGT GTGCAGTCCT GAAGGAATGT 
GGCAGCATCT CTGAGCTGGA CCTCTCAAAT AATTACCTGG GAGATGAAGG ACTTGCACAG 
CTGTTGCAGT TCCTCCCGAA TCTGAAAACG TTGAGGTCAC TCAAGCTAAA TAACAATCAC 
CTCTCCCTGA GCTCTGTGTT CTGCTTAGCT CAGTCCTTGT GTACACTGGA ACACTTGGAA 
ACAATGGACC TCAGCTTAGG ACGCATGCAG GTGGTTCATC TAACCTTTGG GGACAGAATC 
AGGCTCAGAA GGACCAGCAG ATGGAGAAGT TGTTTCCTGG TGCATCCGAA GCACGTAACA 
AATGGACAGT GCTTTCGTCT CAGGAACTGC ACGGTGGGTC CTGAGGACGT GACCAGGCTC 
TGCCAGATGC TGATGCAGTG TACCCAGCTG ACGGAAATCG ATCTGTGTGG AAATGCACTG 
AATGACCAAA GCATTGAGAG ATTACTGAGC TTCCTGCCAC ACTTCTGCCA GCTGACACTA 
CTGAGTATTA GGAACAACAC CTTTTCCCCA TGTTGTGCTG TATTATTTGC CAACTCGATC 
AATCTCTGTG AGCGCATCAG AAGGGTGGAA GTCCGGTCAA GTCAGAATGC TTTTTTACAC 
TTAAGAACAA GCACGCAGGG CCAGAAGACA TCCTGCAGGC TGACTGACTG TGCCATCGGC 
CAAAGGCAGA TAGAGAAGCT CTGCAGAGTC CTGGAGCAGC ATGGGTGTCT GGCAGAACTA 
GATCTCTCCA GGAATCAGCT GGGAGATGAA GTCTTGAGGT TCCTCTTGGA CCACTTGCAT 
CGAGTGCCTG TTACCTGTTC TCTCAATCTC AGCCATAATA GGATTTCTCA GCGTGGAGTT 
CTGCATCTCA TAAATACTTT TACCACTTCT GGAAATACCA CCGAAGTCCA GGTCAGTCTG 
TGCTCCAAGG CAACTTTAAT CATCAAGATG ACAAGCAGAG ATGACCCAAG AAAGATTTTG 
AGGCTCACAG AGTGCAGCTT TCAGCCAGAA CACTTGGAAA AGCTATGTTT GGTTCTGGAG 
AACTGCACCA ATCTGACAGA GTGCATATCC TCCAACAACA ACGTGACAGT CGATGCTGCA 
GGGGGGCTTT TACGTTCACT GAGTAAAACT CCAGGTCCTC TAAAAATCAG CATAGAAGAG 
CCCTGGGTAT GTAAGTTGAG TGTCACGAGC CTTCTTGAGC TGGCTGTCCA GGCCCATGGA 
AACATCACTG CGATCATGAT ATGCAAAGAG AAGAACCTCT TCCAACTAGG TGTAAGGCTT 
CCACACTGCC TGGAGAAGGT GGGATCAGTC GTTAGCAGAT TGAATTTGAA CGAGCCAGAA 
ATCAAACAGG CTTGCTTCTA CCAAAGGGTT CATGACAAAT GCACCCAGCT TCAGGAACTG 
AGATGGTCTC ACGTTGAACT CCACGATGAC ACAGAAATGC TCGTCAGTAT TTTGCTACCT 
CTTCCAGATC TGAAGAAGTT TGAGCTGACC TCTTGCAGCT TTACACCAAC TGGAATTGAT 
TGCTTGATCA CAGGCTTGCA GAGGTGCCAG GCCATTGAAG AGCTCAACCT GGGCCACATG 
AAGCTCGGCG ATGCTGCCAT TCCTAAGCTA GTGTTTGGAC TCTGTGAAAT GCCGTCTCTG 
AAAAGGCTGA TCTTGAACCA TAACAGTATT GGTGACGATG GCTGCTCCAG ACTCGCAGAA 
GCCCTGAGCA GCATGCACTG CATGGAAGAA ATCAATTTAG GCCACAACAA GATTGGAGAT 
CTAGGCCTGA TAAATATAGC TGCTGTCCTG CTGGAAATGC AAAACTTGAA GAGAATCGAC 
CTCTCAGGGA ACTGTCCCAG TCCTGCTGGA GGAGAAAAGC TGATGGAAGC TCTTGCCAAT 
TGCAAGCACC TCGAGGAGCT AATACTGTCA AGGAATGATT TTGGAGATGG GACAGCAGTG 
AAACTTGCCC TCTGTCTGCC TCACATGAAC AGGCTGAAGA TCCTGCACTT GCAGCACAAT 
AACATCGGGC CAGCAGGAGG AACGGAGCTG GCCAGAGCCC TGATGGCATG TGAACTGTTA 
GAAGAGATAA GTTTATCTGA AAACAACCTT GGGGAAGGAG GTATCCGTGC CCTGTCCGAG 
GGGCTGCCGC GTTTTGAGCA CCTCCGGAAG ATTGAGTTGA GACTCTGTGG AATCACTGAT 
GATGCTTCAA AATTACTCTC TCGTGGCTTC CAGCAATGCC CTGCTGTGGA GGAGATCATA 
TTGTCTTGGA ACGCCCTTGG TGATGGAGGA GCCCAAGTGT TGGCCAGCAT CCTCCCAGGG 
ATGGAAACGC TGAAGATGTT AGACCTGGAG AAAAATCTCA TTGGTGCGTG TGGTGCTACC 
AGGTTGGCAG AGGAACTTGT CAGGTGCCCA GAAATTCAGT TCATAAGGCT GTGGGACAAT 
CCGGTGCCCA AAGGCCTCGC TGAAAGTTTG ACAAGCCAAG ACCCAAGACT GTGCTTCTCC 
TTCTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147813.1
CDS	250..5838
Translation	MQPTLQNDDFNLEGAVASIRPQLVEFLSHRLDWLLSASQHFLPTAVLSGLAGVTDHREKVSVLLDLLEKAGHATWKQFAQCLCMECDLPLEMEILLMSSAGEGNLTQKQEAQTDASDQSSVPRGLTRRRRSSSPISDKGAKQKRLDSAEKYQRLLVDSICKRYGSRRAAGAAAQEPTQPLAFSQAFVNLVLRQSKASRLKERTDKPREDVPSAPEPEECVDTAVRVSDLFGSVVRSGTTKVIFLFGKPGTGKTMLMHRICQKWAEGVLHQFLFTFLFEFRQLNLLKRKLTLKELLFDLFLQPEDSPDAVFQHLLENAQRTLIIFDGLDEFVGNMDVSSASKGSLNRSSSMSISELFAELCHGNLLPGCTVLVTSRPKRVPDFLINTVDLLAEVWGFDHEKVEEYVSHYFRHHSFKEQAIAHLKNNTKLLSMCQIPALCYVVCICLEYLLLKHQTSVELPQTMTQFYIKMLLIFINKQQGEHAGDEEAQLNSNKKAILGLCDLALKGLEAKKLVFYVGDIPEHVKEFASLHGLLTVFEVKTNSAHPETGYAFVHLSLQEFFAALCLMISKSVDKSHLKRKLSLKSKWTLRNEAKTGFMESFHIFLSGLSSKECRTFIMLLAEQSEAWVQDKQDAILQSLKKLAATQLTGPKVIELCHCTFETQGLEVAQHIGSLLNFKYEFKNFRLTPLDMSALVFVINSGQDVTHLDFAGCPLDTDCLEMLASCRNVEHLSFRSRRFGDDFAAALSKGLGEMGSLKKLEVTGGSITAAGLTDMVQALSHCLQLEEINLQDNRIQNPDVKTVMELFSRMEKLKKIDLSKNSLSLNAVLLLAKEFIACQNAAELHVRKDTVIISFSGPSGKVPRSLDLKREQNKECVTPTRHLRLCLQARCLSSQHAKEIVSILQSCPHLSEVDLSDNKLGDEGCSFLLENLSWISISKQLNLSHNLLSVTGIYSLLKAVNTCQRVVEVEVSLYHNTAVLRFTEDGEFASPPASREEPLYPTDDQWDDKENQTPVPSKKIRLTYCRFQASDLEKLCAVLKECGSISELDLSNNYLGDEGLAQLLQFLPNLKTLRSLKLNNNHLSLSSVFCLAQSLCTLEHLETMDLSLGRMQVVHLTFGDRIRLRRTSRWRSCFLVHPKHVTNGQCFRLRNCTVGPEDVTRLCQMLMQCTQLTEIDLCGNALNDQSIERLLSFLPHFCQLTLLSIRNNTFSPCCAVLFANSINLCERIRRVEVRSSQNAFLHLRTSTQGQKTSCRLTDCAIGQRQIEKLCRVLEQHGCLAELDLSRNQLGDEVLRFLLDHLHRVPVTCSLNLSHNRISQRGVLHLINTFTTSGNTTEVQVSLCSKATLIIKMTSRDDPRKILRLTECSFQPEHLEKLCLVLENCTNLTECISSNNNVTVDAAGGLLRSLSKTPGPLKISIEEPWVCKLSVTSLLELAVQAHGNITAIMICKEKNLFQLGVRLPHCLEKVGSVVSRLNLNEPEIKQACFYQRVHDKCTQLQELRWSHVELHDDTEMLVSILLPLPDLKKFELTSCSFTPTGIDCLITGLQRCQAIEELNLGHMKLGDAAIPKLVFGLCEMPSLKRLILNHNSIGDDGCSRLAEALSSMHCMEEINLGHNKIGDLGLINIAAVLLEMQNLKRIDLSGNCPSPAGGEKLMEALANCKHLEELILSRNDFGDGTAVKLALCLPHMNRLKILHLQHNNIGPAGGTELARALMACELLEEISLSENNLGEGGIRALSEGLPRFEHLRKIELRLCGITDDASKLLSRGFQQCPAVEEIILSWNALGDGGAQVLASILPGMETLKMLDLEKNLIGACGATRLAEELVRCPEIQFIRLWDNPVPKGLAESLTSQDPRLCFSFC

Target ORF information:

RefSeq Version	XM_015292327.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NLR family, CARD domain containing 5 (NLRC5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292327.1

ORF Insert Sequence:

ATGCAGCCCA CACTCCAGAA CGATGACTTC AACCTGGAAG GAGCTGTAGC AAGCATCCGC 
CCCCAGCTAG TGGAGTTCCT CAGCCATCGT CTCGACTGGC TGCTCTCTGC TTCCCAGCAC 
TTCCTCCCTA CGGCTGTCCT CAGCGGCTTG GCAGGCGTCA CTGACCACAG AGAGAAAGTC 
TCTGTGCTCC TCGACCTGCT GGAGAAGGCT GGCCATGCCA CCTGGAAACA GTTTGCACAG 
TGCCTCTGCA TGGAGTGTGA CCTGCCCCTG GAAATGGAGA TACTGCTCAT GAGTTCTGCA 
GGAGAAGGTA ACTTGACTCA GAAGCAAGAA GCTCAGACAG ACGCAAGTGA CCAGTCCTCT 
GTGCCAAGAG GGTTGACGCG CCGCCGCCGT AGCAGCAGCC CCATCAGTGA TAAGGGTGCT 
AAACAGAAAC GCCTGGACTC AGCTGAAAAG TATCAACGTC TTCTCGTTGA CTCCATCTGC 
AAGAGATATG GAAGCAGGAG AGCAGCAGGA GCAGCGGCAC AAGAGCCAAC GCAGCCTCTG 
GCTTTCAGCC AAGCCTTTGT CAACCTCGTT CTTCGGCAGA GCAAGGCCTC CCGACTGAAA 
GAGAGAACAG ACAAACCCCG GGAGGACGTG CCCAGTGCCC CCGAGCCAGA AGAATGTGTG 
GATACAGCCG TGAGAGTGTC AGATCTCTTT GGCAGCGTGG TCCGATCGGG TACAACGAAG 
GTGATCTTTT TGTTTGGTAA ACCAGGTACA GGCAAGACCA TGCTGATGCA CAGGATTTGT 
CAGAAGTGGG CGGAAGGTGT CTTACATCAG TTTCTCTTCA CTTTCCTGTT TGAGTTCCGG 
CAGTTGAATT TACTAAAAAG AAAACTGACT CTGAAGGAGC TTTTGTTTGA CCTGTTTCTT 
CAGCCAGAAG ACAGCCCCGA TGCAGTGTTC CAGCACCTCC TGGAAAACGC CCAACGTACA 
CTGATCATCT TTGATGGGCT GGATGAGTTT GTGGGTAACA TGGACGTGTC ATCAGCTTCT 
AAAGGAAGCC TGAACCGTAG CTCCAGTATG TCCATATCTG AACTCTTTGC AGAGCTTTGT 
CATGGGAACC TCCTACCTGG CTGCACGGTG TTGGTCACCA GCCGCCCTAA GAGAGTCCCT 
GACTTCTTGA TAAACACAGT AGATTTGCTG GCAGAAGTTT GGGGGTTTGA TCACGAGAAG 
GTTGAGGAGT ATGTCAGCCA CTATTTTCGT CATCATTCAT TCAAAGAACA AGCCATCGCT 
CACCTGAAGA ACAACACCAA GCTCCTCAGC ATGTGCCAGA TCCCTGCCCT GTGTTACGTT 
GTCTGCATCT GTTTGGAGTA TTTGCTCCTT AAGCATCAGA CGAGCGTGGA GCTTCCCCAG 
ACCATGACAC AGTTTTATAT CAAAATGCTT CTCATCTTCA TAAACAAACA GCAGGGGGAG 
CATGCAGGTG ATGAGGAGGC TCAGCTGAAC AGTAACAAGA AGGCCATTTT GGGTCTGTGT 
GATCTTGCAC TGAAAGGCCT GGAAGCTAAG AAGCTCGTGT TTTACGTTGG AGATATTCCA 
GAGCACGTGA AAGAGTTTGC TTCCTTGCAC GGTCTGCTGA CTGTCTTTGA GGTGAAGACA 
AACAGCGCTC ACCCCGAGAC TGGCTATGCC TTTGTGCACT TGAGCCTGCA GGAGTTTTTT 
GCAGCCCTGT GTCTCATGAT AAGCAAGAGT GTGGACAAGA GCCACCTGAA GAGGAAGTTA 
TCTCTGAAGT CAAAGTGGAC GTTAAGGAAT GAAGCAAAGA CTGGGTTCAT GGAGAGCTTT 
CACATCTTCC TCTCAGGCTT GTCATCCAAG GAGTGTAGGA CGTTTATCAT GTTGCTAGCT 
GAACAAAGTG AAGCTTGGGT GCAGGATAAG CAAGATGCAA TCCTTCAGTC CCTCAAGAAA 
CTGGCAGCCA CTCAGCTGAC CGGGCCCAAA GTCATTGAGC TCTGCCATTG CACGTTTGAA 
ACGCAAGGCC TCGAAGTAGC CCAGCACATT GGGAGCCTGC TGAATTTCAA GTACGAGTTT 
AAAAACTTCA GACTGACACC TCTGGACATG TCGGCTTTGG TTTTTGTCAT CAACTCGGGC 
CAGGATGTGA CTCACTTGGA TTTTGCAGGG TGTCCCTTAG ACACGGACTG TTTGGAGATG 
CTGGCCAGCT GTAGGAATGT GGAGCATCTG AGCTTTCGGA GTAGGAGATT TGGTGATGAC 
TTTGCTGCTG CGCTTTCAAA GGGCTTAGGA GAAATGGGGA GCCTGAAGAA ACTAGAGGTG 
ACAGGTGGAA GCATCACAGC TGCGGGGCTG ACTGACATGG TCCAAGCTTT GTCACACTGC 
CTGCAGCTCG AGGAAATAAA CTTGCAGGAC AATCGGATAC AGAATCCAGA CGTAAAGACA 
GTGATGGAGC TGTTTTCCAG AATGGAAAAG CTGAAGAAAA TAGATCTGAG CAAGAACAGT 
CTTTCCTTGA ATGCTGTTCT CCTTTTGGCA AAGGAGTTCA TCGCGTGTCA GAATGCTGCT 
GAGCTACACG TCAGGAAGGA CACTGTGATA ATATCTTTCT CGGGCCCATC TGGGAAAGTT 
CCAAGGTCTT TGGATTTGAA GCGGGAACAA AACAAGGAAT GTGTGACTCC AACTAGACAT 
CTGAGGTTGT GCTTGCAGGC TCGCTGCTTG TCTTCACAAC ATGCAAAGGA GATTGTGTCC 
ATTCTGCAGA GCTGCCCACA CCTCTCTGAA GTAGATTTAT CAGATAACAA GCTTGGGGAT 
GAAGGCTGCA GTTTCCTGCT GGAAAACCTC TCCTGGATTT CAATTTCAAA GCAGCTGAAT 
CTGAGTCACA ACCTGCTGTC TGTTACTGGC ATTTACAGTC TGTTGAAGGC AGTGAATACC 
TGTCAGAGGG TGGTGGAGGT GGAAGTCAGC CTTTATCATA ACACTGCAGT CCTGAGGTTT 
ACAGAAGATG GAGAATTTGC TTCCCCTCCT GCTAGTAGGG AAGAGCCTTT GTACCCTACT 
GATGACCAGT GGGATGACAA AGAAAACCAG ACACCTGTTC CCTCCAAAAA AATAAGATTG 
ACATACTGTC GTTTTCAAGC CAGTGACCTG GAGAAGCTGT GTGCAGTCCT GAAGGAATGT 
GGCAGCATCT CTGAGCTGGA CCTCTCAAAT AATTACCTGG GAGATGAAGG ACTTGCACAG 
CTGTTGCAGT TCCTCCCGAA TCTGAAAACG TTGAGGTCAC TCAAGCTAAA TAACAATCAC 
CTCTCCCTGA GCTCTGTGTT CTGCTTAGCT CAGTCCTTGT GTACACTGGA ACACTTGGAA 
ACAATGGACC TCAGCTTAGG ACGCATGCAG GTGGTTCATC TAACCTTTGG GGACAGAATC 
AGGCTCAGAA GGACCAGCAG ATGGAGAAGT TGTTTCCTGG TGCATCCGAA GCACGTAACA 
AATGGACAGT GCTTTCGTCT CAGGAACTGC ACGGTGGGTC CTGAGGACGT GACCAGGCTC 
TGCCAGATGC TGATGCAGTG TACCCAGCTG ACGGAAATCG ATCTGTGTGG AAATGCACTG 
AATGACCAAA GCATTGAGAG ATTACTGAGC TTCCTGCCAC ACTTCTGCCA GCTGACACTA 
CTGAGTATTA GGAACAACAC CTTTTCCCCA TGTTGTGCTG TATTATTTGC CAACTCGATC 
AATCTCTGTG AGCGCATCAG AAGGGTGGAA GTCCGGTCAA GTCAGAATGC TTTTTTACAC 
TTAAGAACAA GCACGCAGGG CCAGAAGACA TCCTGCAGGC TGACTGACTG TGCCATCGGC 
CAAAGGCAGA TAGAGAAGCT CTGCAGAGTC CTGGAGCAGC ATGGGTGTCT GGCAGAACTA 
GATCTCTCCA GGAATCAGCT GGGAGATGAA GTCTTGAGGT TCCTCTTGGA CCACTTGCAT 
CGAGTGCCTG TTACCTGTTC TCTCAATCTC AGCCATAATA GGATTTCTCA GCGTGGAGTT 
CTGCATCTCA TAAATACTTT TACCACTTCT GGAAATACCA CCGAAGTCCA GGTCAGTCTG 
TGCTCCAAGG CAACTTTAAT CATCAAGATG ACAAGCAGAG ATGACCCAAG AAAGATTTTG 
AGGCTCACAG AGTGCAGCTT TCAGCCAGAA CACTTGGAAA AGCTATGTTT GGTTCTGGAG 
AACTGCACCA ATCTGACAGA GTGCATATCC TCCAACAACA ACGTGACAGT CGATGCTGCA 
GGGGGGCTTT TACGTTCACT GAGTAAAACT CCAGGTCCTC TAAAAATCAG CATAGAAGAG 
CCCTGGGTAT GTAAGTTGAG TGTCACGAGC CTTCTTGAGC TGGCTGTCCA GGCCCATGGA 
AACATCACTG CGATCATGAT ATGCAAAGAG AAGAACCTCT TCCAACTAGG TGTAAGGCTT 
CCACACTGCC TGGAGAAGGT GGGATCAGTC GTTAGCAGAT TGAATTTGAA CGAGCCAGAA 
ATCAAACAGG CTTGCTTCTA CCAAAGGGTT CATGACAAAT GCACCCAGCT TCAGGAACTG 
AGATGGTCTC ACGTTGAACT CCACGATGAC ACAGAAATGC TCGTCAGTAT TTTGCTACCT 
CTTCCAGATC TGAAGAAGTT TGAGCTGACC TCTTGCAGCT TTACACCAAC TGGAATTGAT 
TGCTTGATCA CAGGCTTGCA GAGGTGCCAG GCCATTGAAG AGCTCAACCT GGGCCACATG 
AAGCTCGGCG ATGCTGCCAT TCCTAAGCTA GTGTTTGGAC TCTGTGAAAT GCCGTCTCTG 
AAAAGGCTGA TCTTGAACCA TAACAGTATT GGTGACGATG GCTGCTCCAG ACTCGCAGAA 
GCCCTGAGCA GCATGCACTG CATGGAAGAA ATCAATTTAG GCCACAACAA GATTGGAGAT 
CTAGGCCTGA TAAATATAGC TGCTGTCCTG CTGGAAATGC AAAACTTGAA GAGAATCGAC 
CTCTCAGGGA ACTGTCCCAG TCCTGCTGGA GGAGAAAAGC TGATGGAAGC TCTTGCCAAT 
TGCAAGCACC TCGAGGAGCT AATACTGTCA AGGAATGATT TTGGAGATGG GACAGCAGTG 
AAACTTGCCC TCTGTCTGCC TCACATGAAC AGGCTGAAGA TCCTGCACTT GCAGCACAAT 
AACATCGGGC CAGCAGGAGG AACGGAGCTG GCCAGAGCCC TGATGGCATG TGAACTGTTA 
GAAGAGATAA GTTTATCTGA AAACAACCTT GGGGAAGGAG GTATCCGTGC CCTGTCCGAG 
GGGCTGCCGC GTTTTGAGCA CCTCCGGAAG ATTGAGTTGA GACTCTGTGG AATCACTGAT 
GATGCTTCAA AATTACTCTC TCGTGGCTTC CAGCAATGCC CTGCTGTGGA GGAGATCATA 
TTGTCTTGGA ACGCCCTTGG TGATGGAGGA GCCCAAGTGT TGGCCAGCAT CCTCCCAGGG 
ATGGAAACGC TGAAGATGTT AGACCTGGAG AAAAATCTCA TTGGTGCGTG TGGTGCTACC 
AGGTTGGCAG AGGAACTTGT CAGGTGCCCA GAAATTCAGT TCATAAGGCT GTGGGACAAT 
CCGGTGCCCA AAGGCCTCGC TGAAAGTTTG ACAAGCCAAG ACCCAAGACT GTGCTTCTCC 
TTCTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRC5 ( XM_004944056.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004944113.1 Gallus gallus NLR family, CARD domain containing 5 (NLRC5), transcript variant X5, mRNA.

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CloneID	OGa13512
Clone ID Related Accession (Same CDS sequence)	XM_004944056.2
Accession Version	XM_004944056.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	protein NLRC5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455926.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004944056.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCA CACTCCAGAA CGATGACTTC AACCTGGAAG GAGCTGTAGC AAGCATCCGC 
CCCCAGCTAG TGGAGTTCCT CAGCCATCGT CTCGACTGGC TGCTCTCTGC TTCCCAGCAC 
TTCCTCCCTA CGGCTGTCCT CAGCGGCTTG GCAGGCGTCA CTGACCACAG AGAGAAAGTC 
TCTGTGCTCC TCGACCTGCT GGAGAAGGCT GGCCATGCCA CCTGGAAACA GTTTGCACAG 
TGCCTCTGCA TGGAGTGTGA CCTGCCCCTG GAAATGGAGA TACTGCTCAT GAGTTCTGCA 
GGAGAAGGTA ACTTGACTCA GAAGCAAGAA GCTCAGACAG ACGCAAGTGA CCAGTCCTCT 
GTGCCAAGAG GGTTGACGCG CCGCCGCCGT AGCAGCAGCC CCATCAGTGA TAAGGGTGCT 
AAACAGAAAC GCCTGGACTC AGCTGAAAAG TATCAACGTC TTCTCGTTGA CTCCATCTGC 
AAGAGATATG GAAGCAGGAG AGCAGCAGGA GCAGCGGCAC AAGAGCCAAC GCAGCCTCTG 
GCTTTCAGCC AAGCCTTTGT CAACCTCGTT CTTCGGCAGA GCAAGGCCTC CCGACTGAAA 
GAGAGAACAG ACAAACCCCG GGAGGACGTG CCCAGTGCCC CCGAGCCAGA AGAATGTGTG 
GATACAGCCG TGAGAGTGTC AGATCTCTTT GGCAGCGTGG TCCGATCGGG TACAACGAAG 
GTGATCTTTT TGTTTGGTAA ACCAGGTACA GGCAAGACCA TGCTGATGCA CAGGATTTGT 
CAGAAGTGGG CGGAAGGTGT CTTACATCAG TTTCTCTTCA CTTTCCTGTT TGAGTTCCGG 
CAGTTGAATT TACTAAAAAG AAAACTGACT CTGAAGGAGC TTTTGTTTGA CCTGTTTCTT 
CAGCCAGAAG ACAGCCCCGA TGCAGTGTTC CAGCACCTCC TGGAAAACGC CCAACGTACA 
CTGATCATCT TTGATGGGCT GGATGAGTTT GTGGGTAACA TGGACGTGTC ATCAGCTTCT 
AAAGGAAGCC TGAACCGTAG CTCCAGTATG TCCATATCTG AACTCTTTGC AGAGCTTTGT 
CATGGGAACC TCCTACCTGG CTGCACGGTG TTGGTCACCA GCCGCCCTAA GAGAGTCCCT 
GACTTCTTGA TAAACACAGT AGATTTGCTG GCAGAAGTTT GGGGGTTTGA TCACGAGAAG 
GTTGAGGAGT ATGTCAGCCA CTATTTTCGT CATCATTCAT TCAAAGAACA AGCCATCGCT 
CACCTGAAGA ACAACACCAA GCTCCTCAGC ATGTGCCAGA TCCCTGCCCT GTGTTACGTT 
GTCTGCATCT GTTTGGAGTA TTTGCTCCTT AAGCATCAGA CGAGCGTGGA GCTTCCCCAG 
ACCATGACAC AGTTTTATAT CAAAATGCTT CTCATCTTCA TAAACAAACA GCAGGGGGAG 
CATGCAGGTG ATGAGGAGGC TCAGCTGAAC AGTAACAAGA AGGCCATTTT GGGTCTGTGT 
GATCTTGCAC TGAAAGGCCT GGAAGCTAAG AAGCTCGTGT TTTACGTTGG AGATATTCCA 
GAGCACGTGA AAGAGTTTGC TTCCTTGCAC GGTCTGCTGA CTGTCTTTGA GGTGAAGACA 
AACAGCGCTC ACCCCGAGAC TGGCTATGCC TTTGTGCACT TGAGCCTGCA GGAGTTTTTT 
GCAGCCCTGT GTCTCATGAT AAGCAAGAGT GTGGACAAGA GCCACCTGAA GAGGAAGTTA 
TCTCTGAAGT CAAAGTGGAC GTTAAGGAAT GAAGCAAAGA CTGGGTTCAT GGAGAGCTTT 
CACATCTTCC TCTCAGGCTT GTCATCCAAG GAGTGTAGGA CGTTTATCAT GTTGCTAGCT 
GAACAAAGTG AAGCTTGGGT GCAGGATAAG CAAGATGCAA TCCTTCAGTC CCTCAAGAAA 
CTGGCAGCCA CTCAGCTGAC CGGGCCCAAA GTCATTGAGC TCTGCCATTG CACGTTTGAA 
ACGCAAGGCC TCGAAGTAGC CCAGCACATT GGGAGCCTGC TGAATTTCAA GTACGAGTTT 
AAAAACTTCA GACTGACACC TCTGGACATG TCGGCTTTGG TTTTTGTCAT CAACTCGGGC 
CAGGATGTGA CTCACTTGGA TTTTGCAGGG TGTCCCTTAG ACACGGACTG TTTGGAGATG 
CTGGCCAGCT GTAGGAATGT GGAGCATCTG AGCTTTCGGA GTAGGAGATT TGGTGATGAC 
TTTGCTGCTG CGCTTTCAAA GGGCTTAGGA GAAATGGGGA GCCTGAAGAA ACTAGAGGTG 
ACAGGTGGAA GCATCACAGC TGCGGGGCTG ACTGACATGG TCCAAGCTTT GTCACACTGC 
CTGCAGCTCG AGGAAATAAA CTTGCAGGAC AATCGGATAC AGAATCCAGA CGTAAAGACA 
GTGATGGAGC TGTTTTCCAG AATGGAAAAG CTGAAGAAAA TAGATCTGAG CAAGAACAGT 
CTTTCCTTGA ATGCTGTTCT CCTTTTGGCA AAGGAGTTCA TCGCGTGTCA GAATGCTGCT 
GAGCTACACG TCAGGAAGGA CACTGTGATA ATATCTTTCT CGGGCCCATC TGGGAAAGTT 
CCAAGGTCTT TGGATTTGAA GCGGGAACAA AACAAGGAAT GTGTGACTCC AACTAGACAT 
CTGAGGTTGT GCTTGCAGGC TCGCTGCTTG TCTTCACAAC ATGCAAAGGA GATTGTGTCC 
ATTCTGCAGA GCTGCCCACA CCTCTCTGAA GTAGATTTAT CAGATAACAA GCTTGGGGAT 
GAAGGCTGCA GTTTCCTGCT GGAAAACCTC TCCTGGATTT CAATTTCAAA GCAGCTGAAT 
CTGAGTCACA ACCTGCTGTC TGTTACTGGC ATTTACAGTC TGTTGAAGGC AGTGAATACC 
TGTCAGAGGG TGGTGGAGGT GGAAGTCAGC CTTTATCATA ACACTGCAGT CCTGAGGTTT 
ACAGAAGATG GAGAATTTGC TTCCCCTCCT GCTAGGGAAG AGCCTTTGTA CCCTACTGAT 
GACCAGTGGG ATGACAAAGA AAACCAGACA CCTGTTCCCT CCAAAAAAAT AAGATTGACA 
TACTGTCGTT TTCAAGCCAG TGACCTGGAG AAGCTGTGTG CAGTCCTGAA GGAATGTGGC 
AGCATCTCTG AGCTGGACCT CTCAAATAAT TACCTGGGAG ATGAAGGACT TGCACAGCTG 
TTGCAGTTCC TCCCGAATCT GAAAACGTTG AGGTCACTCA AGCTAAATAA CAATCACCTC 
TCCCTGAGCT CTGTGTTCTG CTTAGCTCAG TCCTTGTGTA CACTGGAACA CTTGGAAACA 
ATGGACCTCA GCTTAGGACG CATGCAGGTG GTTCATCTAA CCTTTGGGGA CAGAATCAGG 
CTCAGAAGGA CCAGCAGATG GAGAAGTTGT TTCCTGGTGC ATCCGAAGCA CGTAACAAAT 
GGACAGTGCT TTCGTCTCAG GAACTGCACG GTGGGTCCTG AGGACGTGAC CAGGCTCTGC 
CAGATGCTGA TGCAGTGTAC CCAGCTGACG GAAATCGATC TGTGTGGAAA TGCACTGAAT 
GACCAAAGCA TTGAGAGATT ACTGAGCTTC CTGCCACACT TCTGCCAGCT GACACTACTG 
AGTATTAGGA ACAACACCTT TTCCCCATGT TGTGCTGTAT TATTTGCCAA CTCGATCAAT 
CTCTGTGAGC GCATCAGAAG GGTGGAAGTC CGGTCAAGTC AGAATGCTTT TTTACACTTA 
AGAACAAGCA CGCAGGGCCA GAAGACATCC TGCAGGCTGA CTGACTGTGC CATCGGCCAA 
AGGCAGATAG AGAAGCTCTG CAGAGTCCTG GAGCAGCATG GGTGTCTGGC AGAACTAGAT 
CTCTCCAGGA ATCAGCTGGG AGATGAAGTC TTGAGGTTCC TCTTGGACCA CTTGCATCGA 
GTGCCTGTTA CCTGTTCTCT CAATCTCAGC CATAATAGGA TTTCTCAGCG TGGAGTTCTG 
CATCTCATAA ATACTTTTAC CACTTCTGGA AATACCACCG AAGTCCAGGT CAGTCTGTGC 
TCCAAGGCAA CTTTAATCAT CAAGATGACA AGCAGAGATG ACCCAAGAAA GATTTTGAGG 
CTCACAGAGT GCAGCTTTCA GCCAGAACAC TTGGAAAAGC TATGTTTGGT TCTGGAGAAC 
TGCACCAATC TGACAGAGTG CATATCCTCC AACAACAACG TGACAGTCGA TGCTGCAGGG 
GGGCTTTTAC GTTCACTGAG TAAAACTCCA GGTCCTCTAA AAATCAGCAT AGAAGAGCCC 
TGGGTATGTA AGTTGAGTGT CACGAGCCTT CTTGAGCTGG CTGTCCAGGC CCATGGAAAC 
ATCACTGCGA TCATGATATG CAAAGAGAAG AACCTCTTCC AACTAGGTGT AAGGCTTCCA 
CACTGCCTGG AGAAGGTGGG ATCAGTCGTT AGCAGATTGA ATTTGAACGA GCCAGAAATC 
AAACAGGCTT GCTTCTACCA AAGGGTTCAT GACAAATGCA CCCAGCTTCA GGAACTGAGA 
TGGTCTCACG TTGAACTCCA CGATGACACA GAAATGCTCG TCAGTATTTT GCTACCTCTT 
CCAGATCTGA AGAAGTTTGA GCTGACCTCT TGCAGCTTTA CACCAACTGG AATTGATTGC 
TTGATCACAG GCTTGCAGAG GTGCCAGGCC ATTGAAGAGC TCAACCTGGG CCACATGAAG 
CTCGGCGATG CTGCCATTCC TAAGCTAGTG TTTGGACTCT GTGAAATGCC GTCTCTGAAA 
AGGCTGATCT TGAACCATAA CAGTATTGGT GACGATGGCT GCTCCAGACT CGCAGAAGCC 
CTGAGCAGCA TGCACTGCAT GGAAGAAATC AATTTAGGCC ACAACAAGAT TGGAGATCTA 
GGCCTGATAA ATATAGCTGC TGTCCTGCTG GAAATGCAAA ACTTGAAGAG AATCGACCTC 
TCAGGGAACT GTCCCAGTCC TGCTGGAGGA GAAAAGCTGA TGGAAGCTCT TGCCAATTGC 
AAGCACCTCG AGGAGCTAAT ACTGTCAAGG AATGATTTTG GAGATGGGAC AGCAGTGAAA 
CTTGCCCTCT GTCTGCCTCA CATGAACAGG CTGAAGATCC TGCACTTGCA GCACAATAAC 
ATCGGGCCAG CAGGAGGAAC GGAGCTGGCC AGAGCCCTGA TGGCATGTGA ACTGTTAGAA 
GAGATAAGTT TATCTGAAAA CAACCTTGGG GAAGGAGGTA TCCGTGCCCT GTCCGAGGGG 
CTGCCGCGTT TTGAGCACCT CCGGAAGATT GAGTTGAGAC TCTGTGGAAT CACTGATGAT 
GCTTCAAAAT TACTCTCTCG TGGCTTCCAG CAATGCCCTG CTGTGGAGGA GATCATATTG 
TCTTGGAACG CCCTTGGTGA TGGAGGAGCC CAAGTGTTGG CCAGCATCCT CCCAGGGATG 
GAAACGCTGA AGATGTTAGA CCTGGAGAAA AATCTCATTG GTGCGTGTGG TGCTACCAGG 
TTGGCAGAGG AACTTGTCAG GTGCCCAGAA ATTCAGTTCA TAAGGCTGTG GGACAATCCG 
GTGCCCAAAG GCCTCGCTGA AAGTTTGACA AGCCAAGACC CAAGACTGTG CTTCTCCTTC 
TGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004944113.1
CDS	281..5866
Translation	MQPTLQNDDFNLEGAVASIRPQLVEFLSHRLDWLLSASQHFLPTAVLSGLAGVTDHREKVSVLLDLLEKAGHATWKQFAQCLCMECDLPLEMEILLMSSAGEGNLTQKQEAQTDASDQSSVPRGLTRRRRSSSPISDKGAKQKRLDSAEKYQRLLVDSICKRYGSRRAAGAAAQEPTQPLAFSQAFVNLVLRQSKASRLKERTDKPREDVPSAPEPEECVDTAVRVSDLFGSVVRSGTTKVIFLFGKPGTGKTMLMHRICQKWAEGVLHQFLFTFLFEFRQLNLLKRKLTLKELLFDLFLQPEDSPDAVFQHLLENAQRTLIIFDGLDEFVGNMDVSSASKGSLNRSSSMSISELFAELCHGNLLPGCTVLVTSRPKRVPDFLINTVDLLAEVWGFDHEKVEEYVSHYFRHHSFKEQAIAHLKNNTKLLSMCQIPALCYVVCICLEYLLLKHQTSVELPQTMTQFYIKMLLIFINKQQGEHAGDEEAQLNSNKKAILGLCDLALKGLEAKKLVFYVGDIPEHVKEFASLHGLLTVFEVKTNSAHPETGYAFVHLSLQEFFAALCLMISKSVDKSHLKRKLSLKSKWTLRNEAKTGFMESFHIFLSGLSSKECRTFIMLLAEQSEAWVQDKQDAILQSLKKLAATQLTGPKVIELCHCTFETQGLEVAQHIGSLLNFKYEFKNFRLTPLDMSALVFVINSGQDVTHLDFAGCPLDTDCLEMLASCRNVEHLSFRSRRFGDDFAAALSKGLGEMGSLKKLEVTGGSITAAGLTDMVQALSHCLQLEEINLQDNRIQNPDVKTVMELFSRMEKLKKIDLSKNSLSLNAVLLLAKEFIACQNAAELHVRKDTVIISFSGPSGKVPRSLDLKREQNKECVTPTRHLRLCLQARCLSSQHAKEIVSILQSCPHLSEVDLSDNKLGDEGCSFLLENLSWISISKQLNLSHNLLSVTGIYSLLKAVNTCQRVVEVEVSLYHNTAVLRFTEDGEFASPPAREEPLYPTDDQWDDKENQTPVPSKKIRLTYCRFQASDLEKLCAVLKECGSISELDLSNNYLGDEGLAQLLQFLPNLKTLRSLKLNNNHLSLSSVFCLAQSLCTLEHLETMDLSLGRMQVVHLTFGDRIRLRRTSRWRSCFLVHPKHVTNGQCFRLRNCTVGPEDVTRLCQMLMQCTQLTEIDLCGNALNDQSIERLLSFLPHFCQLTLLSIRNNTFSPCCAVLFANSINLCERIRRVEVRSSQNAFLHLRTSTQGQKTSCRLTDCAIGQRQIEKLCRVLEQHGCLAELDLSRNQLGDEVLRFLLDHLHRVPVTCSLNLSHNRISQRGVLHLINTFTTSGNTTEVQVSLCSKATLIIKMTSRDDPRKILRLTECSFQPEHLEKLCLVLENCTNLTECISSNNNVTVDAAGGLLRSLSKTPGPLKISIEEPWVCKLSVTSLLELAVQAHGNITAIMICKEKNLFQLGVRLPHCLEKVGSVVSRLNLNEPEIKQACFYQRVHDKCTQLQELRWSHVELHDDTEMLVSILLPLPDLKKFELTSCSFTPTGIDCLITGLQRCQAIEELNLGHMKLGDAAIPKLVFGLCEMPSLKRLILNHNSIGDDGCSRLAEALSSMHCMEEINLGHNKIGDLGLINIAAVLLEMQNLKRIDLSGNCPSPAGGEKLMEALANCKHLEELILSRNDFGDGTAVKLALCLPHMNRLKILHLQHNNIGPAGGTELARALMACELLEEISLSENNLGEGGIRALSEGLPRFEHLRKIELRLCGITDDASKLLSRGFQQCPAVEEIILSWNALGDGGAQVLASILPGMETLKMLDLEKNLIGACGATRLAEELVRCPEIQFIRLWDNPVPKGLAESLTSQDPRLCFSFC

Target ORF information:

RefSeq Version	XM_004944056.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NLR family, CARD domain containing 5 (NLRC5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004944056.2

ORF Insert Sequence:

ATGCAGCCCA CACTCCAGAA CGATGACTTC AACCTGGAAG GAGCTGTAGC AAGCATCCGC 
CCCCAGCTAG TGGAGTTCCT CAGCCATCGT CTCGACTGGC TGCTCTCTGC TTCCCAGCAC 
TTCCTCCCTA CGGCTGTCCT CAGCGGCTTG GCAGGCGTCA CTGACCACAG AGAGAAAGTC 
TCTGTGCTCC TCGACCTGCT GGAGAAGGCT GGCCATGCCA CCTGGAAACA GTTTGCACAG 
TGCCTCTGCA TGGAGTGTGA CCTGCCCCTG GAAATGGAGA TACTGCTCAT GAGTTCTGCA 
GGAGAAGGTA ACTTGACTCA GAAGCAAGAA GCTCAGACAG ACGCAAGTGA CCAGTCCTCT 
GTGCCAAGAG GGTTGACGCG CCGCCGCCGT AGCAGCAGCC CCATCAGTGA TAAGGGTGCT 
AAACAGAAAC GCCTGGACTC AGCTGAAAAG TATCAACGTC TTCTCGTTGA CTCCATCTGC 
AAGAGATATG GAAGCAGGAG AGCAGCAGGA GCAGCGGCAC AAGAGCCAAC GCAGCCTCTG 
GCTTTCAGCC AAGCCTTTGT CAACCTCGTT CTTCGGCAGA GCAAGGCCTC CCGACTGAAA 
GAGAGAACAG ACAAACCCCG GGAGGACGTG CCCAGTGCCC CCGAGCCAGA AGAATGTGTG 
GATACAGCCG TGAGAGTGTC AGATCTCTTT GGCAGCGTGG TCCGATCGGG TACAACGAAG 
GTGATCTTTT TGTTTGGTAA ACCAGGTACA GGCAAGACCA TGCTGATGCA CAGGATTTGT 
CAGAAGTGGG CGGAAGGTGT CTTACATCAG TTTCTCTTCA CTTTCCTGTT TGAGTTCCGG 
CAGTTGAATT TACTAAAAAG AAAACTGACT CTGAAGGAGC TTTTGTTTGA CCTGTTTCTT 
CAGCCAGAAG ACAGCCCCGA TGCAGTGTTC CAGCACCTCC TGGAAAACGC CCAACGTACA 
CTGATCATCT TTGATGGGCT GGATGAGTTT GTGGGTAACA TGGACGTGTC ATCAGCTTCT 
AAAGGAAGCC TGAACCGTAG CTCCAGTATG TCCATATCTG AACTCTTTGC AGAGCTTTGT 
CATGGGAACC TCCTACCTGG CTGCACGGTG TTGGTCACCA GCCGCCCTAA GAGAGTCCCT 
GACTTCTTGA TAAACACAGT AGATTTGCTG GCAGAAGTTT GGGGGTTTGA TCACGAGAAG 
GTTGAGGAGT ATGTCAGCCA CTATTTTCGT CATCATTCAT TCAAAGAACA AGCCATCGCT 
CACCTGAAGA ACAACACCAA GCTCCTCAGC ATGTGCCAGA TCCCTGCCCT GTGTTACGTT 
GTCTGCATCT GTTTGGAGTA TTTGCTCCTT AAGCATCAGA CGAGCGTGGA GCTTCCCCAG 
ACCATGACAC AGTTTTATAT CAAAATGCTT CTCATCTTCA TAAACAAACA GCAGGGGGAG 
CATGCAGGTG ATGAGGAGGC TCAGCTGAAC AGTAACAAGA AGGCCATTTT GGGTCTGTGT 
GATCTTGCAC TGAAAGGCCT GGAAGCTAAG AAGCTCGTGT TTTACGTTGG AGATATTCCA 
GAGCACGTGA AAGAGTTTGC TTCCTTGCAC GGTCTGCTGA CTGTCTTTGA GGTGAAGACA 
AACAGCGCTC ACCCCGAGAC TGGCTATGCC TTTGTGCACT TGAGCCTGCA GGAGTTTTTT 
GCAGCCCTGT GTCTCATGAT AAGCAAGAGT GTGGACAAGA GCCACCTGAA GAGGAAGTTA 
TCTCTGAAGT CAAAGTGGAC GTTAAGGAAT GAAGCAAAGA CTGGGTTCAT GGAGAGCTTT 
CACATCTTCC TCTCAGGCTT GTCATCCAAG GAGTGTAGGA CGTTTATCAT GTTGCTAGCT 
GAACAAAGTG AAGCTTGGGT GCAGGATAAG CAAGATGCAA TCCTTCAGTC CCTCAAGAAA 
CTGGCAGCCA CTCAGCTGAC CGGGCCCAAA GTCATTGAGC TCTGCCATTG CACGTTTGAA 
ACGCAAGGCC TCGAAGTAGC CCAGCACATT GGGAGCCTGC TGAATTTCAA GTACGAGTTT 
AAAAACTTCA GACTGACACC TCTGGACATG TCGGCTTTGG TTTTTGTCAT CAACTCGGGC 
CAGGATGTGA CTCACTTGGA TTTTGCAGGG TGTCCCTTAG ACACGGACTG TTTGGAGATG 
CTGGCCAGCT GTAGGAATGT GGAGCATCTG AGCTTTCGGA GTAGGAGATT TGGTGATGAC 
TTTGCTGCTG CGCTTTCAAA GGGCTTAGGA GAAATGGGGA GCCTGAAGAA ACTAGAGGTG 
ACAGGTGGAA GCATCACAGC TGCGGGGCTG ACTGACATGG TCCAAGCTTT GTCACACTGC 
CTGCAGCTCG AGGAAATAAA CTTGCAGGAC AATCGGATAC AGAATCCAGA CGTAAAGACA 
GTGATGGAGC TGTTTTCCAG AATGGAAAAG CTGAAGAAAA TAGATCTGAG CAAGAACAGT 
CTTTCCTTGA ATGCTGTTCT CCTTTTGGCA AAGGAGTTCA TCGCGTGTCA GAATGCTGCT 
GAGCTACACG TCAGGAAGGA CACTGTGATA ATATCTTTCT CGGGCCCATC TGGGAAAGTT 
CCAAGGTCTT TGGATTTGAA GCGGGAACAA AACAAGGAAT GTGTGACTCC AACTAGACAT 
CTGAGGTTGT GCTTGCAGGC TCGCTGCTTG TCTTCACAAC ATGCAAAGGA GATTGTGTCC 
ATTCTGCAGA GCTGCCCACA CCTCTCTGAA GTAGATTTAT CAGATAACAA GCTTGGGGAT 
GAAGGCTGCA GTTTCCTGCT GGAAAACCTC TCCTGGATTT CAATTTCAAA GCAGCTGAAT 
CTGAGTCACA ACCTGCTGTC TGTTACTGGC ATTTACAGTC TGTTGAAGGC AGTGAATACC 
TGTCAGAGGG TGGTGGAGGT GGAAGTCAGC CTTTATCATA ACACTGCAGT CCTGAGGTTT 
ACAGAAGATG GAGAATTTGC TTCCCCTCCT GCTAGGGAAG AGCCTTTGTA CCCTACTGAT 
GACCAGTGGG ATGACAAAGA AAACCAGACA CCTGTTCCCT CCAAAAAAAT AAGATTGACA 
TACTGTCGTT TTCAAGCCAG TGACCTGGAG AAGCTGTGTG CAGTCCTGAA GGAATGTGGC 
AGCATCTCTG AGCTGGACCT CTCAAATAAT TACCTGGGAG ATGAAGGACT TGCACAGCTG 
TTGCAGTTCC TCCCGAATCT GAAAACGTTG AGGTCACTCA AGCTAAATAA CAATCACCTC 
TCCCTGAGCT CTGTGTTCTG CTTAGCTCAG TCCTTGTGTA CACTGGAACA CTTGGAAACA 
ATGGACCTCA GCTTAGGACG CATGCAGGTG GTTCATCTAA CCTTTGGGGA CAGAATCAGG 
CTCAGAAGGA CCAGCAGATG GAGAAGTTGT TTCCTGGTGC ATCCGAAGCA CGTAACAAAT 
GGACAGTGCT TTCGTCTCAG GAACTGCACG GTGGGTCCTG AGGACGTGAC CAGGCTCTGC 
CAGATGCTGA TGCAGTGTAC CCAGCTGACG GAAATCGATC TGTGTGGAAA TGCACTGAAT 
GACCAAAGCA TTGAGAGATT ACTGAGCTTC CTGCCACACT TCTGCCAGCT GACACTACTG 
AGTATTAGGA ACAACACCTT TTCCCCATGT TGTGCTGTAT TATTTGCCAA CTCGATCAAT 
CTCTGTGAGC GCATCAGAAG GGTGGAAGTC CGGTCAAGTC AGAATGCTTT TTTACACTTA 
AGAACAAGCA CGCAGGGCCA GAAGACATCC TGCAGGCTGA CTGACTGTGC CATCGGCCAA 
AGGCAGATAG AGAAGCTCTG CAGAGTCCTG GAGCAGCATG GGTGTCTGGC AGAACTAGAT 
CTCTCCAGGA ATCAGCTGGG AGATGAAGTC TTGAGGTTCC TCTTGGACCA CTTGCATCGA 
GTGCCTGTTA CCTGTTCTCT CAATCTCAGC CATAATAGGA TTTCTCAGCG TGGAGTTCTG 
CATCTCATAA ATACTTTTAC CACTTCTGGA AATACCACCG AAGTCCAGGT CAGTCTGTGC 
TCCAAGGCAA CTTTAATCAT CAAGATGACA AGCAGAGATG ACCCAAGAAA GATTTTGAGG 
CTCACAGAGT GCAGCTTTCA GCCAGAACAC TTGGAAAAGC TATGTTTGGT TCTGGAGAAC 
TGCACCAATC TGACAGAGTG CATATCCTCC AACAACAACG TGACAGTCGA TGCTGCAGGG 
GGGCTTTTAC GTTCACTGAG TAAAACTCCA GGTCCTCTAA AAATCAGCAT AGAAGAGCCC 
TGGGTATGTA AGTTGAGTGT CACGAGCCTT CTTGAGCTGG CTGTCCAGGC CCATGGAAAC 
ATCACTGCGA TCATGATATG CAAAGAGAAG AACCTCTTCC AACTAGGTGT AAGGCTTCCA 
CACTGCCTGG AGAAGGTGGG ATCAGTCGTT AGCAGATTGA ATTTGAACGA GCCAGAAATC 
AAACAGGCTT GCTTCTACCA AAGGGTTCAT GACAAATGCA CCCAGCTTCA GGAACTGAGA 
TGGTCTCACG TTGAACTCCA CGATGACACA GAAATGCTCG TCAGTATTTT GCTACCTCTT 
CCAGATCTGA AGAAGTTTGA GCTGACCTCT TGCAGCTTTA CACCAACTGG AATTGATTGC 
TTGATCACAG GCTTGCAGAG GTGCCAGGCC ATTGAAGAGC TCAACCTGGG CCACATGAAG 
CTCGGCGATG CTGCCATTCC TAAGCTAGTG TTTGGACTCT GTGAAATGCC GTCTCTGAAA 
AGGCTGATCT TGAACCATAA CAGTATTGGT GACGATGGCT GCTCCAGACT CGCAGAAGCC 
CTGAGCAGCA TGCACTGCAT GGAAGAAATC AATTTAGGCC ACAACAAGAT TGGAGATCTA 
GGCCTGATAA ATATAGCTGC TGTCCTGCTG GAAATGCAAA ACTTGAAGAG AATCGACCTC 
TCAGGGAACT GTCCCAGTCC TGCTGGAGGA GAAAAGCTGA TGGAAGCTCT TGCCAATTGC 
AAGCACCTCG AGGAGCTAAT ACTGTCAAGG AATGATTTTG GAGATGGGAC AGCAGTGAAA 
CTTGCCCTCT GTCTGCCTCA CATGAACAGG CTGAAGATCC TGCACTTGCA GCACAATAAC 
ATCGGGCCAG CAGGAGGAAC GGAGCTGGCC AGAGCCCTGA TGGCATGTGA ACTGTTAGAA 
GAGATAAGTT TATCTGAAAA CAACCTTGGG GAAGGAGGTA TCCGTGCCCT GTCCGAGGGG 
CTGCCGCGTT TTGAGCACCT CCGGAAGATT GAGTTGAGAC TCTGTGGAAT CACTGATGAT 
GCTTCAAAAT TACTCTCTCG TGGCTTCCAG CAATGCCCTG CTGTGGAGGA GATCATATTG 
TCTTGGAACG CCCTTGGTGA TGGAGGAGCC CAAGTGTTGG CCAGCATCCT CCCAGGGATG 
GAAACGCTGA AGATGTTAGA CCTGGAGAAA AATCTCATTG GTGCGTGTGG TGCTACCAGG 
TTGGCAGAGG AACTTGTCAG GTGCCCAGAA ATTCAGTTCA TAAGGCTGTG GGACAATCCG 
GTGCCCAAAG GCCTCGCTGA AAGTTTGACA AGCCAAGACC CAAGACTGTG CTTCTCCTTC 
TGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression patterns of NLRC5 and key genes in the STAT1 pathway following infection with Salmonella pullorum.
Gene59723-29(2017 Jan)
Qiu L,Ma T,Chang G,Liu X,Guo X,Xu L,Zhang Y,Zhao W,Xu Q,Chen G

Characterization of the NLRC5 promoter in chicken: SNPs, regulatory elements and CpG islands.
Animal genetics47(5)579-87(2016 Oct)
Guo XM,Liu XP,Chang GB,Xu L,Bi YL,Wang HZ,Zhang Y,Zhu PF,Wu Y,Chen GH

A mutation in the NLRC5 promoter limits NF-?B signaling after Salmonella Enteritidis infection in the spleen of young chickens.
Gene568(2)117-23(2015 Sep)
Chang G,Liu X,Ma T,Xu L,Wang H,Li Z,Guo X,Xu Q,Chen G

NLRC5 knockdown in chicken macrophages alters response to LPS and poly (I:C) stimulation.
BMC veterinary research823(2012 Mar)
Lian L,Ciraci C,Chang G,Hu J,Lamont SJ

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NLRC5 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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